LOCUS       DANLZC010000001        66480 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.001, whole genome shotgun sequence.
ACCESSION   DANLZC010000001 DANLZC010000000
VERSION     DANLZC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 66480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66480
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.001"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(63..938)
                     /locus_tag="PXE44_000001"
     CDS             complement(63..938)
                     /locus_tag="PXE44_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5587560.1"
                     /translation="MWIWFTLLAAVAQAGRNAFQKQLSVNVPVLGVTLARFFYAVPLA
                     SGYLYFLYQYDDNLNIPHFPPLFFVYAIGAGLAQILATSLMVQLFHLKNYAIGVGLAK
                     SEAVWAALLGVLFFGVTISGIGWLGVVLGGVAIFLMTGSGNLRQLSWKTLFLGIASGL
                     CFALTSLWVREASLQLNSHYLLSAAWVLFSVISFEAVILVIYLALLQKETLIKFFRFP
                     KLGMLTSLFSFLGSFGWFNAMSLNHVAFVKTLGQVEIFFILGISYFVFKERLKIQDFI
                     GLILVVIAAILVVLG"
     gene            1046..1882
                     /locus_tag="PXE44_000002"
     CDS             1046..1882
                     /locus_tag="PXE44_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL5587561.1"
                     /translation="MLPHIYIGTGGYADTDLIGTLYPFGTAKEDFLFVYSQHYDTLEI
                     NSTFHAPIGQKAFEGMVQKAEGRLNFSVKLHQDFSHQRTATLDQAQLFLNALAPFTAE
                     GLLANLFVQFPTQFERMPLNRRYLAELCQWFADYPLAIEFRHPSWHIPAVFDTFAKSS
                     NLIWCNVDYPQNIGLPAFHFFANQRTAYLRLHGRNPNWWKGQSAAERHDYRYSDEELK
                     GLAELLFSRRTEFDRLYLYFENTTKSHAFYNIQTLKGFLAEKGFQIKPVPENLLGQQS
                     LF"
     gene            complement(1957..4809)
                     /locus_tag="PXE44_000003"
     CDS             complement(1957..4809)
                     /locus_tag="PXE44_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5587562.1"
                     /translation="MVQAVSFSHFFAIHYFYFFLHKTKGQCTAKPLFSILLFHLFPKN
                     SAKFLLFFYFLVLMVLYSNEVANGKLLITGGTNLNGTLSAEGTSEIILSGRPTPHAYD
                     KVANKEVLIEGDWINRSFNATIFAAKDNGKLEISRNVANINGNFNLTDNATAQIGFTQ
                     GTSQACIRSDRTGVATCNINATLSETDLNSWERTKVAGNVSLADNSTFSLGSKADLTG
                     SITAQESTKVQLNDGSIANLTGLSTTGIFNGANGSTVNLSGVWTTLTNTVVDVLNVAN
                     GSQIHLNTPTATKQNTLTANTLTGNGIFKFVADLTKTASDIVTIKDTASGQFSIEIST
                     NGSAHSAQKIKLFETTNEEDFSLNLANEVRSGEYRYHLLKEGNVYYLNPEKEEEPKIT
                     QKGPSETADPLPEYTGDIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNG
                     NAHLIASELTAQTNAVLGVTTVLDHVIHSEGTQKQNTLTANTLTGNGIFKFVADLTKT
                     ASDIVTIKDTASGQFSIEISTNGSAHSAQKIKLFETTNEEDFSLNLANEVHSGEYRYH
                     LLKEGNVYYLNPEKEEEPKITQKGPSETADPLPEYTGDIEYSEKGPGETADPLPEYTG
                     DIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNGNAHLIASELTAQTNAV
                     LGVTTALDHVIHSEGTQNWKVWSKTDYQTTEHRSAGYGFNQDANVIHLGLEKALDNQV
                     TLGMILSQSKARNNFDHYYSGKGRLTMLSMYAKKTWQNGVFVAIDTGFGKASNRLTYQ
                     ANTVKLDRSVFVTGLSIGKAWESANVNIIPSFSARYHHLSSAGNQLVDAKIETNAVDL
                     LALQAGLSINKTLELNGLQIKPEIGSYFVDASHGKLRTRFNNLQIEQQMGRYFKQEAG
                     ISANYRNINAGIQAGFLTGNTLNKQRYISFKVTYEW"
     gene            complement(4857..5534)
                     /locus_tag="PXE44_000004"
     CDS             complement(4857..5534)
                     /locus_tag="PXE44_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5587563.1"
                     /translation="MNSIIRKEWFTASEVAGMGNLPEQPSNVTRRATKNNWLKRQIEG
                     KRGVAFEYHYSSLPISVQQELGFAQTAVKEEPRTLVAAQTADEMERVPFYNVQASAGF
                     GAFNSEVYAPDDYIGLSKRWLDVRGFYLSYLAFITASGDSMYPTISDGDMLLVNRATT
                     QPKDGKIYVFRQGEQLWVKRVQGIMGGIRLISDNKELYSPIDVIFDETLDFEVIGQVV
                     YIGHEMI"
     gene            5748..5966
                     /locus_tag="PXE44_000005"
     CDS             5748..5966
                     /locus_tag="PXE44_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5587564.1"
                     /translation="MARNKRVRDMSNFEIRGELMKIGKSLSQLGIENGLAKTTVRNAL
                     DKPYPKGEQIIAKAIGKTPQEIWPSRYE"
     gene            5976..7946
                     /locus_tag="PXE44_000006"
     CDS             5976..7946
                     /locus_tag="PXE44_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HDL5587565.1"
                     /translation="MKEWFLAKEIAGLSGVPELPNSVSRLATKEGWQKRQIQGVRGVT
                     YEYHLTSLPIETQQQLRLNAALAVIPQAAELQPKRDDPALIARLNNATDKGRDKAKGK
                     AEACMQLQAFLDQGFSYTQAEAGAATAKNVSQGSLKNWYYKVKGHPVHLWQAILISES
                     GRSKKPQLKAKITEEAWDCFLADYLRPEKPDLRASYRRTQAIAKQYGWQMASLQTFQR
                     RVLAEVPYEVILLKREGANAVAKLVPALQRTVKDILAGEWINGDGYQHNVFVKWHTGE
                     IVRPKTWFWQDVRTRKILGYRTSISENTDSIRHALMDVIFNVGIPKTLTLDNTRAAAN
                     KAMTGGIENRYRFKHTELDPKGIMPILGIDVHFTSILYGEGHGQAKPIERAFGRGGIG
                     EKIDKRPELSGFYTGRNAQETPDNYNGGKDGVDYNTFLKAIAAGIEEYNSQTERHTEM
                     CRGELSFNQVWERDYHPSNVRQASPEQLRLLFLQAETVSIKRNGSFTLKAAGKLYGLT
                     NVYWAESLIGITDKKVVARFDPDDLHGNVYVYNLEGQFLAEAVCREAKGFGDTSASRE
                     QGRLYQKVVKSAKAQAEALELLEAHELASLAPQVDVPEPIEKKVKEVLVKEDFIVDFN
                     TVRKATVVEETEEISIFDEVLLKQEKQLRIVE"
     gene            complement(8126..8614)
                     /locus_tag="PXE44_000007"
     CDS             complement(8126..8614)
                     /locus_tag="PXE44_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587566.1"
                     /translation="MLFRSLTDDEIHQIALDSARATFSGFKLKRANAIVMKNCITLFK
                     LSTINFCLAEYHSSFHHLTSIDDLDGIMPTVKKVAQMLSLQESKALIWLLYLKWLFVM
                     RQRSLQVLLENSADLIQGEVIAQPLPTFVKAVLAFQLETYHGLPEWIPEFDSAEFWQS
                     LS"
     gene            8646..9587
                     /locus_tag="PXE44_000008"
     CDS             8646..9587
                     /locus_tag="PXE44_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5587567.1"
                     /translation="MTLIDQIKQHIAESGSSQGKVAKEAGINAGALSAYLNENYKGNN
                     EELEAKLIAYLERIEAKKREFVEAPSFIETKTANQIFGSLRFAQNTGVLAIIHGASGV
                     GKTQAAREYRKRYANVWLVTASPSRSSLSEVLYEIALELGMNDAPRRKGTLARLIVRK
                     MKGTAGLLIIDEADHLPYEALEELRLLQEEVVNESDIGAGVGLVLIGNDKVYTRMKGG
                     INPAHEYGRLWSRSAKRTSIQKTKKEDTQAVAKAWGLSENQEALKVMQAITETGGGLR
                     ILTHTLRLAAMVAKDRMIPLTADLIQLARKDLLSTGD"
     gene            9590..9904
                     /locus_tag="PXE44_000009"
     CDS             9590..9904
                     /locus_tag="PXE44_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587568.1"
                     /translation="MRTPKPKHSFNRTNQAACRFLKQTQKAIIRLNSLGFTVLNIDFT
                     RIKPRIEVEIGNNKHIAQGLIYEGKAYRYSFGKSEDLGRWEGYYTMLEGIRVWWRQAA
                     IH"
     gene            9916..10152
                     /locus_tag="PXE44_000010"
     CDS             9916..10152
                     /locus_tag="PXE44_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587569.1"
                     /translation="MKTLALLTALLGLVGCDLVQSKQAKNYIGNNLTEICIDNVVYLI
                     YSGDKKGGITPKVNKDFYPYTCTNKEERQNGKTR"
     gene            10136..10429
                     /locus_tag="PXE44_000011"
     CDS             10136..10429
                     /locus_tag="PXE44_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587570.1"
                     /translation="MAKPDETPITKAECQSQLAELGVQYKKLPMAITRHICNATTNIH
                     GKVIKVSVVERVGYGVQITAQGNEKSCLVTYEAMLGMAEAMGLFDEVKEQNND"
     gene            10551..10769
                     /locus_tag="PXE44_000012"
     CDS             10551..10769
                     /locus_tag="PXE44_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL5587571.1"
                     /translation="MNDELAAISASYAQHNRYQHYAEKAAEAERKGNYKEAQDHWEVA
                     KLSAKKTANRDWAEQRAEFCKRMHNKPF"
     gene            10779..10961
                     /locus_tag="PXE44_000013"
     CDS             10779..10961
                     /locus_tag="PXE44_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587572.1"
                     /translation="MTETVKAQLNSQLNEAIIQLIQAQKYLNQDDAIRSGVYIGTVQD
                     LLPKVHLKLLTANRKH"
     gene            10978..11366
                     /locus_tag="PXE44_000014"
                     /pseudo
     CDS             10978..11366
                     /locus_tag="PXE44_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249673.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11415..11702
                     /locus_tag="PXE44_000015"
     CDS             11415..11702
                     /locus_tag="PXE44_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587573.1"
                     /translation="MNKNEFFKKYHLEGSLAILDLDANDDQSQFCYQALLEYRELPLD
                     TFLAEYSDDEDMTESEFIELMLDELEWIIRRAKNAKRFVKKRQKTMREEIM"
     gene            11821..12366
                     /locus_tag="PXE44_000016"
     CDS             11821..12366
                     /locus_tag="PXE44_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HDL5587574.1"
                     /translation="MTDKAKLIQLIHIAKSQLAMDDLSYREMLKRLTNKTSSTKCTVV
                     ELHKVLHELQTKGAKVKYFAKRSKKPTAYSPATGETAVKSQIAHKIRAVWITMGKHGF
                     LNDPSEKALNAYMRKVINNKTRPTILLNVSALEAGDASYMLEILKKWHKRVMIKALSK
                     SCTVDPKISYDELVEWYNEVM"
     gene            12353..12886
                     /locus_tag="PXE44_000017"
     CDS             12353..12886
                     /locus_tag="PXE44_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587575.1"
                     /translation="MKLCRCPICHSDIHLEALVEDEAGRELLGKISQLTHGVAQPMVA
                     YLGLFKPQKSNLNNSRALKILNDVLALYPCSLLLAQALSETVVSIRKKRQQAMENGQK
                     IEPLPNHNYLKSVYETQKVHFAVVRTGKTERETMKAELEKTRNAIAYVQRFVDLGREE
                     VVKNSPEYQIWLAHKPK"
     gene            13051..13410
                     /locus_tag="PXE44_000018"
     CDS             13051..13410
                     /locus_tag="PXE44_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5587576.1"
                     /translation="MELVDIFEEKAPEVLTDLAKHIEVALEEKCQFSPEKAKQIGIDI
                     AQQMAQNWGGEVIYIPRNLLMALNERDMKIYEEFNGNNHRELARKYNVSMQWVYKIVK
                     KVHKDEVARRQMSMFEM"
     gene            13546..14055
                     /locus_tag="PXE44_000019"
     CDS             13546..14055
                     /locus_tag="PXE44_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL5587577.1"
                     /translation="MSQNLTFRQIFDRLIGHEGGYVNHPQDPGGETHWGITKRTAVAN
                     GYMYAMKNMTREQAYQIYEKAFWQRYRCAELKPAVAYQFFDAVVNHGFGNASRMLQRA
                     VGVADDGIIGNITLQAINAKSESDLLMLFNAERLKFYTKLRNFPTFGKGWVNRVAGNL
                     EYAAQDNEV"
     gene            14058..14327
                     /locus_tag="PXE44_000020"
     CDS             14058..14327
                     /locus_tag="PXE44_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL5587578.1"
                     /translation="MAKLFKELLSTNGTLSTTSTVQFFGTLCVFGLMVYAVITQQPYA
                     ESLLNNVLIYLFGATTAKGVVTSYQAKIKGGIHKQQSTFKEEEGC"
     gene            14321..14668
                     /locus_tag="PXE44_000021"
     CDS             14321..14668
                     /locus_tag="PXE44_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL5587579.1"
                     /translation="MLMQQILGVIGVIAAILSYALFKSWQLKRERKKNAILQAEKQQQ
                     AVEIEQKKAEVKYVYISKKNNEKVTRSSASDIDDKLQQHGYFRDNNRLHGVQSDLSEP
                     CGYAGNETSDSCS"
     gene            14695..14847
                     /locus_tag="PXE44_000022"
     CDS             14695..14847
                     /locus_tag="PXE44_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587580.1"
                     /translation="MKYIKKLRKRWQIWRFLKNHPEVAQRRDLLAIAIKQGVENPVGR
                     ARRREP"
     gene            14847..15182
                     /locus_tag="PXE44_000023"
     CDS             14847..15182
                     /locus_tag="PXE44_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 domain-containing protein"
                     /protein_id="HDL5587581.1"
                     /translation="MDLFTVFWESIRANFGFISALLTIVVTAFWLKLDSKYAKKNDLG
                     ELLEIAKSHDNRLTTLETKVENLPSAVDVEKLKTLVTDVKGDTKATSKQVDSISHQLG
                     LLLEAKLKE"
     gene            15184..15480
                     /locus_tag="PXE44_000024"
     CDS             15184..15480
                     /locus_tag="PXE44_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587582.1"
                     /translation="MSLRDLLTQDQRLVILRSLAEAGYDANESIISDCLDLYGHDISR
                     DLVRTHLSWLEEQGLLTLERLRDGYMVASITQRGLDVAQGRVVVSGVKRPRPKI"
     gene            15503..16075
                     /locus_tag="PXE44_000025"
     CDS             15503..16075
                     /locus_tag="PXE44_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HDL5587583.1"
                     /translation="MSEKNTRGRASKVDLLPPNIKTQLAMMLRDKQYSQSQILEEIND
                     LIRDCGLPDEMLLSKTGLNRYASRMERLGAKIRNAREVAEIWTKQFGEMPQTDIGKTL
                     MEMVKQIAFETSLKLGEQEGGIEPKQLALLSSAIQRLEQAESLSFKREQAIRKEVAQQ
                     AAETAEKVVVQAGLSAETVRTIKEQILGIA"
     gene            16075..17631
                     /locus_tag="PXE44_000026"
     CDS             16075..17631
                     /locus_tag="PXE44_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDL5587584.1"
                     /translation="MALLNNRPLNELAPECRSFLESIHAFNPNELLLGYQKRWIADDS
                     QLKIAEKTRRCGLTWAEAADNALIASTRKSDGGSDVFYIGSNKEMAREYIDAVAMWAK
                     AFNRVAGEIQEEIFEDEDKDILTYVIYFSSGFKVKALSSNPKNLRGMQGVVVIDEAAF
                     HEYLAEVLKAALALTMWGAKVRIISTHNGSENLFNELITDSRAGRKRYSVHTITIEDA
                     CRDGLYQRICQVVTKKTWSPEAEKEWIENLLNDTASEEDALEEYFCVPKFGSGLWLSR
                     ALIERQMSEATPVIRFTAKDGFSLVAEPTRYAEIAEWCELELEPILATLNPDLLHFMG
                     EDFARSGDMTSLVVLAQQQNLVRKVAFIVELGNMPYKQQEQIVMFMLKRLPHFAGGAF
                     DARGNGGYLAEAARDEFSTLIDCVQLSEKWYREHTAPFKAALEDGELEAIPKDADILA
                     DLRSFQVVKGVPRIPDKRTKSADGKSKRHGDTAISLLLAHYASRQLVQMPVEPVSRRP
                     RHSQQLTEGY"
     gene            17750..19204
                     /locus_tag="PXE44_000027"
     CDS             17750..19204
                     /locus_tag="PXE44_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HDL5587585.1"
                     /translation="MAKKNNLISEIATRARSIDYWAFGYYLPNPDPILKRMGKDISVY
                     RELLSDGQVRSGVRRRKAAIKGLEWRITTTNNEKVDEQIYQIFNRLPMNNLITEMLNA
                     ALYGYHISEVIWAKEGELFVPTEIIGKKPEWFMFDDENLLRFRTKENWADGELLPDDK
                     FLLTTQEATQDNPYGLGDLSLCFWAATFKKGGFKYWLEFTEKYGSPWLIGKHPRQAHN
                     TDKERLADALEGMISSAIAVIPEDSSVEIKDAAGKSASTDAFEKFLNFCKAEINIALL
                     GQNQTTEQESNRASAQVGLEVMEDIRSDDQAMIEATFNTLLQWIVKYNFNVEQLPKFE
                     FYEQEAINTDQVERDSKLHAMGVRFSKAYFEREYGFEAGDIEVQAVEKAEKSANTTEF
                     SEGQRRTQVPMPVPILEQLEHEGEAMVDDWLQGVRDKLAQADSLEDFQSQLDSLIPEL
                     SFAEYGELLALGSTVAELAGRQSVNDEIKAKRNA"
     gene            19197..20474
                     /locus_tag="PXE44_000028"
     CDS             19197..20474
                     /locus_tag="PXE44_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL5587586.1"
                     /translation="MRKFTFKQQAQYFEKKLNLRTNSYLDILGEEHDYFFVVAGANRN
                     EVLSEFRKAVDEAIHNGETLENFRKRFDEVVAKTGWEYNGGRNWRTRIIYDTNVYGAY
                     NRGRLQQHLELAEDMPYWEYQHNDNAHPRPQHVAWDGLVLRYDDPFWKYHYPIKAYGC
                     HCTVQALDDFDLKHDGKTVSPPPEIEFEQKTVGVRTGNSRVVTVPKGVDVGFTPHNFD
                     NLKAGRTANVDKLLMQKMVTADPIFASKAVSDLLAKEPVAKLHNQAFKNWVNDVANAK
                     GSALSQVENMQLLGVLPVAVIERLQQINLAPQSAIIAITKGDMVHALRELKQNTGIAV
                     PLEFWEKLPAKLQYPNAILLEKQQAETTLLFIYDVAQTKIAVKVNYDATLQDRHGAKG
                     RQKVLLNRAVTGSLFQDTTGLKSSKYEVLLGEM"
     gene            complement(20676..21020)
                     /locus_tag="PXE44_000029"
     CDS             complement(20676..21020)
                     /locus_tag="PXE44_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587587.1"
                     /translation="MMVNAEQAIELVYDLLLSRQWLVTKAEKLPLDPLSEKEAVMFLY
                     TLDQQTEASWLQLTPEQRATANGLIMDFIAKCLTSTKQWLVSDNIVPELQAIEIIKHE
                     IFLSHNSLVMPN"
     gene            21084..21548
                     /locus_tag="PXE44_000030"
     CDS             21084..21548
                     /locus_tag="PXE44_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL5587588.1"
                     /translation="MIKITLNNTQAVKSLQQIANQLEQPRRLYGILGETLKKIHTARF
                     KAEQDPDGNNWKPLASSTLALKRKRGKSTKILRQDGYLADKTAYNVTSDNVEFGSQEV
                     YARLHQFGGKAGRGKKVTVPARPWLGVGDKDEQLLLRKAEWHLGQIIGRMGK"
     gene            21783..22898
                     /locus_tag="PXE44_000031"
     CDS             21783..22898
                     /locus_tag="PXE44_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL5587589.1"
                     /translation="MTQIEIFKAGKRYDANGTLIDITPEMLQQTVAAYNPEFHEAPLV
                     IGHPKSNNPAWGWVKALSLEGDVLKAEVDQLDAEFSEMVASGKFKKVSAAFYLPDSPN
                     NPHKGVLSLRHVGFLGAMPPAVKGLKQVEFAEDDDFVVFSEWGVASLFSRFRDWLVSE
                     FGLEKADKALPPHEIDWLKEEAMRSQISKQVHAEQATPEPIFNEPAQPEGEPEMSAEE
                     KEELERLKAENEQLKAEKAQAEAEKMEAELEAEKAGNAEFCEGLVKQGKLAPVAKAAF
                     VQALNNLSVLKAGREPEFNEGEDVVSQFKSAMSQSPQIIQFGEYATADKANPQEAEAV
                     EYAEGTDPVSIEADQKVRAYMKAHNVGYAAAFNAIYR"
     gene            22929..23855
                     /locus_tag="PXE44_000032"
     CDS             22929..23855
                     /locus_tag="PXE44_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDL5587590.1"
                     /translation="MTTHNLQKLRVQDPVLTELAQGYHNNELVAETLMPTVEIEKEAG
                     KISTFGRLAFRLPSTVRNLRGSSNRLDPEDIGAIDVALEEHDVEYAIDYREENEAIFS
                     LRQFALNTTQDVIALGREKAVATLALDESRYETDNKVTLSGTSQFSHKDADVFKVFDT
                     AIRAVKRAIGRKPNVCVISGDVWAALKEHPAVVEKLKYSQVAIVTPEVFAKLIGIETV
                     KIGEAVYEESGQLKDIWSKDIVVAYVAPRSTGGKGTVYEPSYGYTVRRQKGLFVDTYK
                     ESGGKIEVVRTTDIHKPHLVGAAAGYLIKAAV"
     gene            23924..24205
                     /locus_tag="PXE44_000033"
     CDS             23924..24205
                     /locus_tag="PXE44_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587591.1"
                     /translation="MAKFIVLHTPILHDGKRYEVGAEIELTAEQAENNAINLKAVEAV
                     PKPPKNASKPEDEPKPEGEPKPEDAVTEDDVKLAEAKIAEAEQPKGNKK"
     gene            24205..24639
                     /locus_tag="PXE44_000034"
     CDS             24205..24639
                     /locus_tag="PXE44_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL5587592.1"
                     /translation="MYVQIDELLTAFSRKIVVQLSNDEPTATEPDLKMLETAVKVANE
                     RIDAALRSRYTLPLADVPTLINQHALTLARYWLYARRPETKMPDTVKETYQQAVKELE
                     QIATGKLHLGIAESSQILEDDVLADNSEYEVRANARIDTSRY"
     gene            24645..25142
                     /locus_tag="PXE44_000035"
     CDS             24645..25142
                     /locus_tag="PXE44_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HDL5587593.1"
                     /translation="MSATQPILEDICQHLRQALPEWEVELFPNDPSTYHLSHINGAVL
                     ISYLASKFEKPRTTQAVLQVRHVQIALTVLTRDLHNDVGALDLLDKLRLLVVGFKPQN
                     CGECWLIDEFFNGSSEETGLWQYQLILQTETQQVQHQAVENLPKFVTAHLRRQDQPGS
                     PGLKT"
     gene            25227..26612
                     /locus_tag="PXE44_000036"
     CDS             25227..26612
                     /locus_tag="PXE44_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDL5587594.1"
                     /translation="MTFHHGSETERVNGGSVPVSVVDSAIIGIVGTAPIGAVNELTVC
                     LTKKDFAKFGTILNQGFTLPDAFDILARYAAGKVYVVNVLDPSRHRTTVTDEVLTLDR
                     NTLTAQTAKTGIIAVTRVKDGANVLNADRYSVDLQTGVVTFVSAPSAPKISYIYADPS
                     KVTEEDIKGGVDSLTGKRKGFELLRDGFNLYGADAKILICPEFDKTASCAAALATLAD
                     QLHAKAYIQLPKGTSLSKAIQGRGPAGTINASASNENVRHFFPYALGSSNTLESLATH
                     AAGLRMLIDVEKGYWFSTSNKELKGVIGLEIPLTARVDDKQSETNLLNAVGITTIFNS
                     FGTGFRLWGNRSSNFPTVTHISNFEVASRTGDIIDESIRQAELQFIDLPIDDALLDSF
                     LETIDTFLRTQKSLVGYSVSLDYEYDLVDAFSRGQVPIVYEYTPKIPGERISNKSVMT
                     RKYLANLVSQR"
     gene            26623..27138
                     /locus_tag="PXE44_000037"
     CDS             26623..27138
                     /locus_tag="PXE44_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL5587595.1"
                     /translation="MSIAINQIVNGNVYINGNSQMGRVNEVKIPDIEFEFIEHKGLGM
                     HGVIKLPAGTNAIEAEITWDSFYPEVRAVMLNPYKYTQLQIRSNLQVFNSQGLAAEEP
                     MVTIMNVSAGKIGGTGHKNKENAEFADTLNVHSIKQTVAGRELLFIDVLANIYRVNGV
                     DVLQKFRTNIG"
     gene            27234..27548
                     /locus_tag="PXE44_000038"
     CDS             27234..27548
                     /locus_tag="PXE44_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL5587596.1"
                     /translation="MANAAQQVLTNLRVQTTYKLQYPIALPDGRVIETLDVRRPKGKD
                     FRLLDEKGFDPEKDGVKILRFYIQQLTTLVPEDIDELDAADIKGLNNLLEDMLIEGKS
                     DK"
     gene            27545..27697
                     /locus_tag="PXE44_000039"
     CDS             27545..27697
                     /locus_tag="PXE44_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL5587597.1"
                     /translation="MSELDTVYADLAWWFKFSPSELLELDLVDIPKWVEQMNRQIKAG
                     YGQILR"
     gene            complement(27676..27978)
                     /locus_tag="PXE44_000040"
     CDS             complement(27676..27978)
                     /locus_tag="PXE44_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587598.1"
                     /translation="MLTKLNNRITEWAGFEACVTRTECTAKWLLAVALVGWSIFYWLD
                     AIQSIQSLSEGVSFVLIYGLILTILAWVGVYLAWGVAILLTLATEPFIWLYRKICP"
     gene            28026..30683
                     /locus_tag="PXE44_000041"
     CDS             28026..30683
                     /locus_tag="PXE44_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5587599.1"
                     /translation="MANNLAIGMVIGASLSSGFHGAFSGAKKKLEQLEQAVAKNQQSH
                     SKLGSELTALRSKQATLYAEMSRASSKGGAGLALMQQEYKKIGKEIANISREQRKWST
                     ELEKSIAKQTKLRNAMERHTQAKENRDELKGKIIGTVASAAMGVGVMKTYMEQEEAAN
                     NLKISMLKADGSFGKFEEIGKIADTLGTDLPGTKKDFYKLAMALKKQGVSDDVLTGGA
                     LKTSAELNVLLDMDQESGGEFLAKFMESHRLNENELPQAADYLQRAMFAGGLSKEQMY
                     ESMKYYAPKLNSMKLTGAENTEKVLAIEAMAGQQGLEGSTFGTGLNMMLSRMNKGPEM
                     LKAAAKGMKAEAREMMEAVGVEFNFWDKNGSFKGIDGMLAEMTKFEKIRKKFGDKGVG
                     LVAEELFGIEGGRLADILAMKGQKGLDEMLSKMREQASLQDRINLKTNTLGAALESLG
                     GAWETFVGDIGSVFAEDIKGFSKGLQSAVEWLVPFVSKHKEAIKFVGGLVAGLVILRG
                     VLQAGSFVIQTVTMAMNALKIAAMTNPIVLVIGAIAAGAYLIYSHWDSIAPWFANLWQ
                     NVTAYFNQFWTWVKGVWSGVTDWVSSVWNGVATFFGQLWTTITSLFSVAWTNIQTIWG
                     GATAWFSGLWDGIKSAAFGMFDSISQKVSSVVASIKSFLGFGDDVDKMGAKIGNVATQ
                     VISKEHSGQLSKTADMAANTGFSSGGFTGQGGKYTPAGIVHKGEYVMTKEATSRLGVG
                     MLDSLNYGGAMGFTQPVLQPILGKQKGFFGSLWDDVKFGANFVGNLLGLNQGSLKTPD
                     FNPNATNASGQNSSIFSNYEPLNRNAVIQQESSQAPQIVVNFNPTINVNGNATQEAQS
                     QIMQQLQMSAYEFEKLLNRVLDQRSRRAY"
     gene            30693..31616
                     /locus_tag="PXE44_000042"
     CDS             30693..31616
                     /locus_tag="PXE44_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5587600.1"
                     /translation="MYFMLGNIAFEPVNLTEFSEQHTAEFAEHAVLKGKPRLQAMGEK
                     LNELNFSLRLHHTIGGVESRYQALLEAKAKQAALALIWGSRYKGDYVIVDISSTTLFT
                     DGRGNALAREMQISLKEFVGDNDGGVLGEALNFGGGSLLGSILPSGAVTTLSQIKTAV
                     SRGVELYNSGKRLVDEVQNTVAIIRQLKNDPATALAYLPAALANLDGALGHFGNLTGS
                     SATLGGLQEKLPAAVVFSEEIGEIYQSLQTMRSSLGNASANSWDNWFTPAESALTEVT
                     DSFDHLAKPVAKMTAWIVLRTDEEAENDTVA"
     gene            31600..31827
                     /locus_tag="PXE44_000043"
     CDS             31600..31827
                     /locus_tag="PXE44_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL5587601.1"
                     /translation="MTQLLKHIVKQGERWDNLSYQYYGNAFEYGRIIDANPHIGFCEV
                     LPIGVTIYIPVLNVKPTNNENMPPWLRGRDA"
     gene            31820..32884
                     /locus_tag="PXE44_000044"
     CDS             31820..32884
                     /locus_tag="PXE44_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587602.1"
                     /translation="MRNVPKPDFSLFYEKTNITAEIEPHLVQLTYIDHLEGQSDELTV
                     EFEDIQGKWIRQWFPTQGDKLKAAIGYQGEFLTDIGEFEIDEVEYSYKPSTINLKALS
                     TGISKANRTLKPKAYENTTLAQVVAKVADNLKLKVVGKIKAIPIKRITQYQERDVEFL
                     ARLAREYHHSFKIVGNQLVFTDKSELGQSEPVLMLEERDTISLRLRDRIKDTAKAVEI
                     SGYDTSGKKVVKKRKKAKALRPNIKQAKSASEDTLKIITRGESQEQIDARGEAALATQ
                     NEDQAAGDIELIGNPKLVAGATILLRNFGVFSGKYLIKSSRHTISRSGGYTTHIEVRM
                     LEFIADDLITLGMEAGNANA"
     gene            32871..33407
                     /locus_tag="PXE44_000045"
     CDS             32871..33407
                     /locus_tag="PXE44_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL5587603.1"
                     /translation="MQTHNFGATYQEGIVSAVDPKTHKVRCKIPALEDLETAWLSFLT
                     PNAGGNQFYCLPDVGELVAMLLDAQGEGGCVFGAIYNEQDPTPVADSDMWVKKFSNGT
                     EISHNRKTGDVVVKTNGHLTATAQGGATINANTVVNGTLHATGKITSDEEVSAPVVKQ
                     GTVELGTHKHGGSPEPNK"
     gene            33462..33824
                     /locus_tag="PXE44_000046"
     CDS             33462..33824
                     /locus_tag="PXE44_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL5587604.1"
                     /translation="MNRQTSIQLTHWQLAPSTDTVEVVQGIDDIHLCIVNILSTQKGT
                     DILRPEFGSDHFRYIDYPEDVAIPNFVREITTALQKWEKRIEVEEVLVDGSAPHFIFT
                     VMWHLTDDVYREIYRTTV"
     gene            33834..34937
                     /locus_tag="PXE44_000047"
     CDS             33834..34937
                     /locus_tag="PXE44_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL5587605.1"
                     /translation="MTEAIKIIDDDVKTVLAEAIADYEKRTGKILQPAHIERSIIQTY
                     AYREQLLRKGINHAFLQTFPQFATGIALDLCGETFGCYRLRDKPARTLLRFSVSGEHT
                     AIVIPKGTQVAINDELYFSTLNDDVITPLITYVEIDAECNQVGVIGNGWESGRVSKLK
                     TALATNYKVTVENIDITSGGLMQEEDEPYRKRILAAPEAFSNCGSIAAYDYHVRSVSQ
                     DIADVNISTPRGGLVRIAVLTQKGLPDTRLLDDIKKHVSAERLRPLCDMVEVIKPTER
                     RYQITARLILLDGYREDLVKAKAREALQLYLSDKTKKLGLDIVPSAIISALRVEGVYD
                     VNLIQPVKTIINETEWANCTAISVEVEEERRDG"
     gene            34930..35496
                     /locus_tag="PXE44_000048"
     CDS             34930..35496
                     /locus_tag="PXE44_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5587606.1"
                     /translation="MASLHYADIIVRDPKYKALADLSKRLNLLDTSQIMTTLVDLLGD
                     ECIPLLAEKWSVTGYDGLLVADSENSKRALIKRAIELHQLKGTPWSIREVLRRLGFGE
                     VEIDEGLKNRLYESTYVSGIPENERWAHYAIRLSNPITNEQARNIRKVLRNFAPARCS
                     LAVLDYKADPIRYNNKATYNGTYNHGSS"
     gene            35506..37785
                     /locus_tag="PXE44_000049"
     CDS             35506..37785
                     /locus_tag="PXE44_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDL5587607.1"
                     /translation="MAGVQETAQWENHVYRIEENDPVHGGENGVTNKPIKHLANRTLY
                     LRRLLTEAGQRINPKKITATSKNSNDLTGHTHEIDHASLTQKGIVQLNASIQSTSTTE
                     SATPSAVKAAYDKAATAEQKATLANEKVEAIKVGGRNLLIQSNTPHNGNAYSVRYALA
                     EAPKVGEEIVVTLWGELGSQRTGIGVYNSQGATELAKLVKIRDGVYQGKGIWRKPMSE
                     GRERTPNDTHLNVYFYPKTATDNNRIERIQLERGSIGTDWTPNEADLKAEISAIRTKL
                     IPVRGGNVATTLNAAKRSDWFREMQIPYQSGWGYYTQAFHNSGTQHNYSNLPLTGRYY
                     FIAELYHNSGYSHLRITYPSLKRTFESAINLNNEDLILDWREVVFLKDGLYQGTFKTT
                     GTLESAYRLRAARDDERYQPYLQMVDMGYDLANPPVGKNKIIGSVDYYVKNGTSESSK
                     ATMMAAAMPDKNVFWEVALWNAAQQRNVLFKGYSKTNNLSIGKTTDNERDRVQVNGTI
                     QATAPADNANNDQVPTTAWVRKMAKILADGKVSKAGDTMTGHLFIKNGEYSAVHTYNT
                     SGWFAKWEAAPLSDSHFAAIVYANDRETVINRVLIPKKNGTVALINDIADTVRAAVSR
                     SDKSRSRTFPLQTGKKYTTTGSVTIYPDGRIVQIFHLKNIKAAWFDYEASAVGDRHRV
                     VNIPLWTAMPNKIFDVDVKTVRPSNNASTYYIEAAEWLSAWQIYGNDTNKSSVNINLS
                     RFRGGKDEDMDLYVTVEGY"
     gene            37786..38388
                     /locus_tag="PXE44_000050"
     CDS             37786..38388
                     /locus_tag="PXE44_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587608.1"
                     /translation="MAYFVNVIDEQGNYELIDDEFIGLYPDLDFNTLPKLTDKQYQTY
                     LAKANGKERFVNGEFVYEQIKVDMQAVISAEKSAKLAEINQKAQAFINDLAKYNETPK
                     FERDTWLEQAKEAKAWVADPTVQTPTLELIAQMRGIPLDTLRQKAYEKAMAYQTVAAI
                     VAGQRQGYEDRLEQAETLEQIQAIEPMYQLPQGANNEQTD"
     gene            38372..38647
                     /locus_tag="PXE44_000051"
     CDS             38372..38647
                     /locus_tag="PXE44_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587609.1"
                     /translation="MSKLTKLKSWSYHVLIAIDQLFNALTGGAADETFSSRCYRGAVL
                     AENPKKRWRFWHSFLNCLFLDPKHCYAAYKAEVHRKQYPKAFRLGGI"
     gene            38647..38934
                     /locus_tag="PXE44_000052"
     CDS             38647..38934
                     /locus_tag="PXE44_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587610.1"
                     /translation="MKKQNLKLVRGDDVSFRFSIKDSTGQPLALKDLWFDLHLVSYQG
                     EVLIALSTDDGSIETAEAGQMVLHFSHQQTARAEWTSANYDLQLTHDYTRV"
     gene            38944..39108
                     /locus_tag="PXE44_000053"
     CDS             38944..39108
                     /locus_tag="PXE44_000053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587611.1"
                     /translation="MTSIIELDLTDDLIIEVQLDSTIITGDSFLANLPDLLQIYNQAK
                     SDSSKELNND"
     gene            39101..39805
                     /locus_tag="PXE44_000054"
     CDS             39101..39805
                     /locus_tag="PXE44_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587612.1"
                     /translation="MTKHKNHENHPIAITFYQLGKDVGDINRRLAVIEQREQSAQPSQ
                     PQAEAVTMPQQDAVAINNINAVLEAVEKYKTFEKYNTQWQERFEKFLSRNPNDYELSE
                     EQRQKLYHTMCQHLWQVNFTEINSKQYELPIPQQFQGKFGYIVKTSGGSTEISFELLS
                     ETHSTYLYPDSYIPEGGGDVMLKPAYIYLVIPDLVAMREPEVVGVSPLKLNILQKHLL
                     TPQKLQEQKPLNTKAN"
     gene            39974..40132
                     /locus_tag="PXE44_000055"
     CDS             39974..40132
                     /locus_tag="PXE44_000055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587613.1"
                     /translation="MNKSLVALAVAAMASKAQTKQQASKQASKQASKQASKQASKQAS
                     KQASKQAL"
     gene            40134..40973
                     /locus_tag="PXE44_000056"
     CDS             40134..40973
                     /locus_tag="PXE44_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587614.1"
                     /translation="MEKVFKQAPLPFSGQKRMFLSHFKQVLNANIEADGKDWTIIDVF
                     GGSGLLAHTAKREKPLARVIYNDFDNYAERLNHIKETNQLRQEIYQIVDEIIPKNKRI
                     SNEIKAKIINKINDFEGFKDLKCLSSWLLFSGEQVATLDELFKHDFWHCVRQSDYPEA
                     TGYLDDIEVVSESFHQLLPRFHDKEKVLLILDPPYLCTRQESYKQENYFDLIDFLRLI
                     DLTKPPYIFFSSTKSEFIRFIHYMVQNRKENWQAFEGAERIVVNASASYNGKYEDNMV
                     YKF"
     gene            complement(41010..43448)
                     /locus_tag="PXE44_000057"
     CDS             complement(41010..43448)
                     /locus_tag="PXE44_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family igA-specific metalloendopeptidase"
                     /protein_id="HDL5587615.1"
                     /translation="MKHTFKLSLLATSIAISLSPTLLASIVRGDVDYQYFRDLAENKG
                     KFFVGATNIPVIDKTGKNIGTFLQVTPTKQVEVESNISPKPNVSLSECFDNESLPGCY
                     EFLYGGGDEDSNDLPVFETVTGETMSIPMIDFSSITRQTGIATLVNPQYVISVNHNQG
                     YRDIQFGDSSSEKADDHHYNYSVVRRNNYMPDPNKERTDLGKGIEKRERNDEGEKLIL
                     DGSGNPTPRWDYHAPRLSKLVTEVAPANEIERTTENVQDFYSVFSDPFIFPMFIRAGS
                     GRQAVVNNERDRLNNGRIEVGNGPFLTGGSVLPVTNGDPSVPGSDFNSRILVAKTENN
                     TINDVFKDHGYGPLTTLGLPGDSGSALFGYDVRTKKWVVLGVYSDYFSENNTLGGDVY
                     KSYWNYHHPHYVRALEKENNAGAINANGARLTWTPSGNTSSIVGGNAPLTVNLADTSL
                     PTLQGHNGNTGDNPWNPQLQHGKTFHILGENNTLSLTENINQGAGAIHFHGNTTVEGM
                     KAGITWLGAGVDIDKDKNVIWKISNPAGDRLSKIGQGTLTINGKGENKGSVSVGDGTV
                     ILNQQADENNKKSAFSELGIVSGRPTVILNSSDQMDPNNIYFGYRGGRLDVNGNDLTF
                     RRIQNSDEGAMVVNHNTAQTANITLTGIVSPTIDQVKVQAITSPDQVSRHLDKDIFTY
                     QRFPHLPTNFIRLKKPLSVAEITAIFNARKVFIDLDFATGLIRENEYFEVLKVTDESA
                     AKQQILAEKIKAAEGTVQGFNGYFGETDPSKTNGRLNITMNSNCSEPTKLRNATTKTT
                     KENRSFTFLILMVF"
     gene            complement(43768..45534)
                     /gene="aspS"
                     /locus_tag="PXE44_000058"
     CDS             complement(43768..45534)
                     /gene="aspS"
                     /locus_tag="PXE44_000058"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL5587616.1"
                     /translation="MMRSHYCGALNRSHLGQTVTLSGWVHRVRNLGRFIFMQIRDREG
                     IVQVFFDEKDEALFKQASALRSEACVQIQGEVIARDESQINKEMATGEIEVLVKNLVV
                     YNNAEVLPLDFNQNNTEEQRLKYRYLDLRRPEMAEKLKTRAKITSFVRRFMDDNGFLD
                     IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDVETSFMTAEEVRALMEAMIHGLWKETLGVDLGKFPMMTWNEA
                     MTRFGSDKPDLRNPLEMVDVADILKSVDFKVFSGPANDPKGRVVALRVPNGAALTRKN
                     IDDYTQFVGIYGAKGLAWLKVNDMNTGLEGIQSPVAKFLNEEVLKALVKRLNLQKDDI
                     VFFGADNWHVATNAIGALRLKVGNDLGLTDTTAWKPLWVIDFPMFERDDEGNLSAMHH
                     PFTSPKELSPEELVQDPENAVANAYDMVINGYEVGGGSVRIFSQQMQQTVFSILGINE
                     EEQKEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTDNIRDVIAFPKTTAAACLMT
                     EAPSYANPKALEELAIRTIPQE"
     gene            complement(45689..46600)
                     /locus_tag="PXE44_000059"
     CDS             complement(45689..46600)
                     /locus_tag="PXE44_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HDL5587617.1"
                     /translation="MLYGLAIVLIPLFLGYLITLKNHRHMAIVNRVVSLCLYLILLVM
                     GISLGQIDNILTELPQIGGTAFSLVVILLLSNIIGLVIYDKLQPMKRDKVNQDQLPSR
                     LHLLIDSFKLIGITLLGGIIGYFTKGVVDFPLHASTYVLEVMIFGVGIQLRNSGIPLR
                     EVFFNKRGIYTSIVMVASSLIGGVISAFTLDLSLAKGLTFASAFGWYSLSSVLVNDAW
                     GPIYGSIAFFNDISREILCLFLVPLFMRQFPSTAVGIGGATSLDCMLPIIQKSGGIQI
                     VPLAISFGFIVNLAAPLLLAVFIGLEG"
     gene            46723..47805
                     /gene="dinB"
                     /locus_tag="PXE44_000060"
     CDS             46723..47805
                     /gene="dinB"
                     /locus_tag="PXE44_000060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL5587618.1"
                     /translation="MSIQAKHKRKIIHIDMDCFYAAVEMRENPALVGKPVAVGGNANQ
                     RGVLTTCNYEARKFGLHSAMPTATALKKCPNLILLPVNMALYKSVSAQIHEIFHRYTD
                     VIEALSLDEAYLDVTECERYFGSATWIAQAIRNDIFNELHLTASAGVAPLKFLAKITS
                     DQNKPNGQCVITPDDVQNFVKNLPLAKIPGVGKVTNEKLKQLGFETCGDIQQAEKAVI
                     YRAFGKFGQRLWDFSHGIDERAVEPYRERKSLAVENTLLHDISTLEQAEKIVADLFDK
                     LVFRLQRNWGDTPLTEFKKLGIKLKFDDFSQTTLERTTDGFAYERFGELLQQIFTRRK
                     ERKVRLIGLSVHFPEKKVNKQLNLWE"
     gene            47893..48762
                     /gene="htpX"
                     /locus_tag="PXE44_000061"
     CDS             47893..48762
                     /gene="htpX"
                     /locus_tag="PXE44_000061"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDL5587619.1"
                     /translation="MAKRVILFLLTNLAITFVLGIVLNLIFKVTGIQGQSTAGILVLS
                     LVFGFSGSLISLFMSKSMALRSVGAEVITQPRNEAEQWLFNTVQRQAQQANIPMPDIA
                     IYHSADVNAFATGATKSNSLVAVSTGLLNSMTKDEAEAVVAHEVAHIANGDMVTMTLL
                     QGVLNTFVIFLARIISTMAASSRDENGNSSTNTLVFWVVDIVLQMVFGVLATMIAMWF
                     SRQREFRADAGSAQLVGKEKMIAALQRLQRVHEPQEMEGSLAAFMINGSRGKELFMSH
                     PPLEKRIEALRNL"
     gene            complement(48816..49310)
                     /locus_tag="PXE44_000062"
     CDS             complement(48816..49310)
                     /locus_tag="PXE44_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5587620.1"
                     /translation="MTEYLTSFFSLFFSEQNQLFTMFLSGFLSATLLPGNSEIIFTTL
                     ASQHLITYGSLSSFSLFNLLLVATLGNSLGSITTYAIGLLLPRPQNTTNRYSQWALAK
                     SEKYGVLVLLFSWLPIVGDIFCGIAGWLRFSLWQSILFIVIGKLLRYLFLLATLYSVL
                     PHIL"
     gene            49511..50335
                     /gene="focA"
                     /locus_tag="PXE44_000063"
     CDS             49511..50335
                     /gene="focA"
                     /locus_tag="PXE44_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDL5587621.1"
                     /translation="MFSPAEMAKIAEDGAVYKATKNQLYSFLSAITAGGFIAIAFVFY
                     TTTQVGASQMPWGMAKLIGGLVFSLGVIMCVVFGAELFTSSTLTIVARASQRINWLQM
                     FKNWVVVYFGNFVGAISIVLLIWFSGQIMAAKGQWGLVILNTAQHKIHHQWFEAFCLG
                     IFCNIMVCIAVWMSYAGKSLTDKAFIMMLPIAMFVASGFEHCVANMFMIPMGIAISHF
                     ASPEFWTAINVDPTQFADLDVSHFVFKNLIPATLGNIVGGVFFIGLMQWFLYIRKH"
     gene            50425..52749
                     /gene="pflB"
                     /locus_tag="PXE44_000064"
     CDS             50425..52749
                     /gene="pflB"
                     /locus_tag="PXE44_000064"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL5587622.1"
                     /translation="MTQLTDAQQKAWEGFAAGEWQTEVNVRDFIQKNYTPYEGDESFL
                     AEVTPATSKLWADVMEMIKVENKTHEPYDIDCDTPSTITSHAPGYIDKSLEKIVGLQT
                     DAPLKRAIMPFGGINMVKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVITGLPDAYGRGRIIGDYRRMALYGADFLMKDKQRQFASLQARLESGEDIQATI
                     QLREEIAEQHRALGKMKEMAASYGFDISGPATNAQEAVQWTYFAYLAAVKSQNGAAMS
                     FGRVSSFLDIYIERDLKAGKITEQEAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMW
                     ATETLAGMGLDGRTLVTKNSFRILHTLYTMGPSPEPNLTILWSEQLPEAFKRYCAKVS
                     IDTSSVQYENDDLMRPDFQNDDYAIACCVSPMIVGKMMQFFGARANLAKTLLYAINGG
                     VDEKSGDQVGPKTEAITSEYLDYDDVMTRLDSFMDWLATQYVTALNIIHFMHDKYAYE
                     AALMALHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPIRTDIEIKNKAGEVVGVAK
                     NVATDFEIEGEYPQFGNNDNRVDDIAVDLVERFMKKIQKLKTYRNATPTQSVLTITSN
                     VVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTF
                     SIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNVLNRDTLLDAVEHPEKY
                     PQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM"
     gene            52984..54009
                     /locus_tag="PXE44_000065"
     CDS             52984..54009
                     /locus_tag="PXE44_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5587623.1"
                     /translation="MSKQDLIRSSVAEYLTFITATGESQVDAVYADENVWLSQKMMAV
                     LYDVDVRTISYHLKKIFSDNELAENSVIQFFWITAQDGKNYHTKHYNLSAIIAVGYKV
                     NSERAVQFRKWATEIVHSFAIKGFAMDDERLKNDGTILGKKYFEEQLARIREIRLSER
                     KFYQKITDIYATAVDYDRTATATKRFFATVQNKLHWAIHGHTAAELIMARANAEKPNM
                     GLTNWKDAPQGKIYPFDVVVAKNYLSDNELAQLQRLVSAYLDMAEDMAERQIPMTMAD
                     WETRLNRFLAATDREILQDAGKVTAEIAKSFALSEFEKYRVKQDLTYESDFDLLVKEV
                     AAKYHAG"
     gene            54258..54998
                     /gene="pflA"
                     /locus_tag="PXE44_000066"
     CDS             54258..54998
                     /gene="pflA"
                     /locus_tag="PXE44_000066"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDL5587624.1"
                     /translation="MSVVGRIHSFESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWD
                     LDGGKEITVEELMKEVTTYKHFMKATGGGVTASGGEAVLQMEFVRDWFRACKAEGINT
                     CLDTNGFVRHYSPVVDEMLEVTDLVLLDLKQLNDEVHQDLVGVSNKRTLDFARYLHKL
                     NKPTWVRYVVVPGYTDDDDSAHRLGQFIQGMDNIEKVELLPYHRLGAHKWETLGYKYE
                     LEGVMSPPKEDLERIQKIIESYGHTVKF"
     gene            55129..57195
                     /gene="prlC"
                     /locus_tag="PXE44_000067"
     CDS             55129..57195
                     /gene="prlC"
                     /locus_tag="PXE44_000067"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDL5587625.1"
                     /translation="MSNPLLNYTGLPAFSQIKPEHIKPAVEAVIKECRETVEKVAQIE
                     NPTWENFYMPQAMAEDKFSRAWSPVGHLNAVKNSPELREAYQACLPLLSEYSTWAGQH
                     QGLYQGYVKLKNSPEFETYSVAQKKAIENSLQDFELSGISLPAEKQKRYGEISARLSE
                     LNSQFSNNVLDATMGWDIVITDEAQLKGLPESALEAAKLSAESKGKKGYRFTLEIPSY
                     LPVMTYCENKALREEMYRAYVTRASDQGPNAGKWDNSAVIDEILKLRHEKAQLLGFKT
                     YSDYSLATKMAENPQQVLDFLNDLAARSKAQGKNELCDLKKFAKAHFGIEHLDLWDIP
                     FYSEKQKQALYSVNDEELRPYFPENRVLSGLFEVIKRIFGMRVEEQKDVDVWHKDVRF
                     FNIFDKNDRLRGSFYLDLYARENKRGGAWMDDCINQKRLADGTLQKPVAYLTCNFNKP
                     IGDKPALFTHDEVTTLFHEFGHGIHHMLTEIDVGDVAGINGVPWDAVELPSQFLENWC
                     WEEEALAFISGHYETGEPLPKAKLEQLLAAKNFQAAMFVLRQLEFGLFDFRLHTEYDP
                     SKQGQVLALLKEVKDQVAVAKGVEWARTPHSFSHIFAGGYAAGYYSYLWAEVLSADAY
                     SRFEEEGIFNPETGQSFLDNILTRGGSEEPMVLFERFRGRKPTLDALLRHKGIVGNAE
                     PVDCTQ"
     gene            57317..58474
                     /locus_tag="PXE44_000068"
     CDS             57317..58474
                     /locus_tag="PXE44_000068"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL5587626.1"
                     /translation="MSLDLADIRTQITNLDRNLLKLLAERHRLAFDVVRSKEITQKPL
                     RDLEREKILLQELVNFAEAENYQLDPQYVTQIFQRIIEDSVLTQQNYLQNKLNAQKEE
                     QVSIAFLGMRGSYSNMASRQFAKKYQGTLAELSCHSFNEVFEKVSLGEADFGVLPLEN
                     TTSGSINEVYDLLQHTDLTLVGELAYPIKHCVLANGTVDLAEVDTIYTHPQPAQQCSQ
                     FLAGLDKVHIKYCESSSHAMQMVARLNKPNIVALGNEDGGKLYGLTNIKTDIANQAHN
                     ITRFIVVAKEAVQVSPQVQTKTLLLMTTTQQAGALVDALMVFKNHHIRMMKLESRPIY
                     GKPWEEMFYVELEANIHSENTQKALAELEQVTSYLKVLGCYPSEIIEPVKL"
     gene            complement(58533..58748)
                     /locus_tag="PXE44_000069"
     CDS             complement(58533..58748)
                     /locus_tag="PXE44_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDL5587627.1"
                     /translation="MFPEFRDLITQLKQNNSHFARLFDKHNELDQRIKNMEANIELAT
                     HEEVEALKKEKLHLKDQLYAILKQESA"
     gene            58938..59753
                     /locus_tag="PXE44_000070"
     CDS             58938..59753
                     /locus_tag="PXE44_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL5587628.1"
                     /translation="MSYKVVFSLLFSLFLSTANANWTKINDVNYTWGPFNIYHITLFT
                     ESGTYVEGTRPLMLTLQYKKPWEGRDFAVSLARTWNNLGIKLPEKELDEVITHLRKTF
                     PDIKPEDTLHYIALEDRGYFILNDKVVSDVFNKAFNDAIVAIWLDPKMDISHQLLDPS
                     YKPRAEAESIKPQVEERVAENLQEPIVNTEVSKELKADSQAVEQAPNKIDIPVADKTQ
                     ASVVNEATQPVENKDKKEPEKLEEKPKEMKIENPEVFISPIYDDILMLTKYCV"
     gene            complement(59815..60459)
                     /gene="adk"
                     /locus_tag="PXE44_000071"
     CDS             complement(59815..60459)
                     /gene="adk"
                     /locus_tag="PXE44_000071"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL5587629.1"
                     /translation="MKVILLGAPGAGKGTQAQFIMNKFGIPQISTGDMFRAAIKESTE
                     LGKQAKALMDEGKLVPDELTVALVKDRIAQPDCANGFLLDGFPRTIPQADALKESGVK
                     IDLVLEFDVADEVIVERMSGRRVHQPSGRTYHVVYNPPKVEGKDDVTGEDLIIRQDDK
                     PETVLERLAIYHKQTKPLIAYYTAEAEAGNTRYERLDGTKPVEEVSAELAKILS"
     gene            complement(60571..62274)
                     /locus_tag="PXE44_000072"
     CDS             complement(60571..62274)
                     /locus_tag="PXE44_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL5587630.1"
                     /translation="MLYDRVEVVRGATGLTTGAGDPSASVNLVRKRANSISRYTILEA
                     GIGRYADFNTMIDHSQALNNSGSVRGRFIAEHKNGNTFIKNEKERYTTLYGNIEADLT
                     DTTKLGLGVSYQKEKRDAALWGGLPAFYSDGGETNWSRSKNASPEWAYWDNESVNYFA
                     DITQKLGEKWELALKGNYRVGKHNSELFYFSGSSLNRQDGLGWSPWPGKFKNDNTQSN
                     VQLDLTGSFSAWGLEHDAAIGIQYNRAHRNSYAADNSTFDGAFDFNNWNGIYARPNWG
                     SMSEKYYQNLKEFGVYVSTRLHLTDRLSMLLGSRISSYRTEGTFYGSTQNFHANDVWT
                     PYAGLTFDINPQNTVYVSYTDIFKPQSQVDINNKLLSPVTGETYEAGWKGSYFNDNLQ
                     TQISVFETRQDNLAQSAGINPNTLLAYYTASKGAKVRGFEVEASGNVTEKLKLSAGYT
                     QWKGDDASGNPINTTQPRKQFKLFATYDMNRLVQGLTVGAGVNWQSRIYTKHATYGEY
                     GQKAYTLVNLMARYQFNDNFSAQLNIDNLFDKKYRNALSFSQYSYGEPFYTRLNLRYE
                     F"
     gene            complement(62309..62707)
                     /locus_tag="PXE44_000073"
     CDS             complement(62309..62707)
                     /locus_tag="PXE44_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5587631.1"
                     /translation="MNTLSKAIKLALYSSGIGFISTAYAETVTHLDTVEVSAETPNAS
                     SEETGRYAQKKAKGSNGMALSAKDTPQSVTAVTHQQIRDQNLNTIAKALEATHGVSVS
                     LLDRGRYSFSARGFGIDKVKVDGMDLKVSK"
     gene            complement(62735..63871)
                     /locus_tag="PXE44_000074"
     CDS             complement(62735..63871)
                     /locus_tag="PXE44_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5587632.1"
                     /translation="MIAFLAGGFLLFFTNNLIGIVSRIYDTLLPVGAFTATLGAPLLI
                     FLVLRQRKAQFEASNTAYFPVKRLNSWKLPILLLCAGVILFCLLQIIAPTMNGWAISI
                     DENLMSSYRLPRSLSAIATGAMLAVSGSLLQILTRNPMASPEVLGVSSGATIAAVLSF
                     LLFPTLSSISFLLFGVLGSLTVLALIMWLSRKLQPSALMITGVAIGALVSGLMAIIQL
                     SGSPRLTAVISWLSGSTYYANPNTVWILLSVAILLISATLLFIKPLRLISLGETVSYH
                     LGLEVKKVQMIVLLLIALMSAASTLAVGPMSFIGLMTPHLAKNLGAITPEKQIPISAL
                     LGAMLMLIADWLGRYLIFPYEIGAGIIASILGGIYFLLLLKQHK"
     gene            complement(63868..64683)
                     /locus_tag="PXE44_000075"
     CDS             complement(63868..64683)
                     /locus_tag="PXE44_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL5587633.1"
                     /translation="MKSSFLLIGCILLSLVTAVIRLNMVWEWEWQLLLINVDLLPINQ
                     AAVKAALLPEMTTSFLAGGLLAIASSGLQHIVRNNLASDSTLAVSSGAQLALMIAVIF
                     FPAAELFSSFWIALLGSLAAIVLVLFIAKASKLNPLTLILGGLIVNILFGAIASLLTL
                     FYFDFLFGVMVWGSGSLLQDGWATSITLAITVVVAFFIFVLLSRPLTILSLDDEQARR
                     LGAPVNLLRYLVIFVCAAITALVVSKIGVIGFIGFAVQVWLILPKYDIYYFAS"
     gene            complement(64683..65564)
                     /locus_tag="PXE44_000076"
     CDS             complement(64683..65564)
                     /locus_tag="PXE44_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5587634.1"
                     /translation="MMFRQYKRFIFLFILQISTAFAQPNIFTPDWSIASALTEMGNPP
                     IAMGDKRIYPLWSHRPIQPNSVLDIGARYQPNRELLAQLPVELVLSNFFYAHLASVYP
                     KEIPQIDVLFDGGNTEPQQHWHTYQKATRMIGTEIKNPNAAEAYLTKTEQTLAQWGKE
                     ILNSNPDIDYYSVVQFGSPRELRIYASNSMFRVAFDLMGLKQADLGVGDRWGNKLIPV
                     SDLAKLPERTCLIVISPFSKMTQADLENSYIWQRLGFGKNRCMKVLPPVWLFGGADSI
                     SHFAEFLHNAMTQGEKE"
     gene            complement(65561..66229)
                     /locus_tag="PXE44_000077"
     CDS             complement(65561..66229)
                     /locus_tag="PXE44_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5587635.1"
                     /translation="MAHNGSGKSTLMRLLSGDITPTSGSILVDGLPLHQLSTKEAAKH
                     IAYLPQRLPEAADFLVQELVALGRFPYQKWLQKPTEFDVQIVEQAMESTSVTQFAKQP
                     VNSLSGGEKQRVWLAMCLAQQTPYLLLDEPLAALDIVYQVEVMQLIRKLVNEQGLTVI
                     IIIHDINLASQFCDEIIALKQGELCIQGSVDEIMQEQKLLEIFGLKLHLFNHPAGLHK
                     VAVL"
BASE COUNT        20463 a        13233 c        14802 g        17982 t
ORIGIN      
        1 attggttttg cgtgggctta cgcccaccct acaaagctag ataaaaaacg taaaaatccg
       61 atctagccca gcaccaccaa gattgcggca atcactacta aaatcagccc tataaaatct
      121 tgaattttaa gccgttcttt aaacacaaaa taggaaatac cgagaataaa gaaaatttcc
      181 acttgtccaa gcgtttttac aaaggcaacg tggttcaggc tcattgcatt gaaccaaccg
      241 aatgagccga gaaatgaaaa caggctggtc aacataccta attttggaaa acgaaagaat
      301 ttaatcagtg tctctttttg gagaagagct aggtatataa ccaatatcac tgcctcaaag
      361 ctaatcacgc taaaaagtac ccacgccgct gaaagcaaat agtggctgtt gagttgcagg
      421 cttgcctctc gcacccaaag tgatgtgagg gcgaaacata aaccgctggc tatgccgaga
      481 aagagtgttt tccacgaaag ttggcgtaga ttaccgctgc cggtcattaa gaaaatcgcc
      541 actccaccta gtaccacgcc aagccagcca atgccactga tggttacacc aaaaaataac
      601 acgcccagta aagccgccca tactgcctcg cttttcgcca gcccgacacc tattgcataa
      661 ttttttaagt gaaaaagttg caccattagc gaagttgcca gaatttgagc caacccagcc
      721 ccgatagcat atacaaaaaa cagcggtgga aaatggggaa tgttgaggtt atcgtcatat
      781 tggtataaaa aatagagata accgcttgcc aacggcacag catagaaaaa gcgggcgagc
      841 gttaccccca atacaggcac attgacgctt aattgctttt gaaaggcatt gcgaccggct
      901 tgagccacgg ctgccagcag agtaaaccaa atccacataa tgatgatgaa ataagaaaag
      961 atagccaaat cttactcttt tattctcagt ttgcaagcgg ttaaaaaacc attaaaattt
     1021 gcaaaaaatt gaaagaataa acacaatgct ccctcatatt tatattggca ccggcggcta
     1081 tgccgacaca gatttaatcg gcacgcttta cccattcggc actgcaaaag aggattttct
     1141 ctttgtctat agccaacatt acgacacgct ggaaattaac agcacattcc acgcccccat
     1201 cgggcagaaa gcctttgaag gaatggtgca gaaagcggaa ggtcggctga atttttcggt
     1261 gaaactgcat caagatttca gccatcaacg cacggcgact ttagatcaag cacaattatt
     1321 tcttaatgcg ttagctccgt tcaccgctga agggttattg gcaaatttat ttgttcagtt
     1381 cccaactcaa tttgaacgta tgccacttaa ccgccgttac cttgccgagt tgtgccaatg
     1441 gtttgcggat tatccgttag cgattgaatt tcgccaccca agctggcata ttccggcggt
     1501 gtttgatacc tttgcgaaaa gctctaatct gatttggtgt aatgtggatt acccacaaaa
     1561 tatcggctta ccggcatttc acttttttgc caaccaacgt actgcttatt tacgcttaca
     1621 tggtcgtaac ccgaattggt ggaaagggca gagtgcggca gaacgccacg attaccgcta
     1681 ttcagacgag gaattaaaag gcttggcaga attgcttttt agccgcagaa cggaatttga
     1741 caggctctat ctctactttg aaaacaccac taaaagccac gccttttata atattcaaac
     1801 cctaaaaggc tttttggcgg aaaaaggatt tcaaatcaaa cctgtgccgg agaatttgct
     1861 tgggcagcag agtttgtttt gatgggtaat ttacattgtt gaggagtaaa taaagggcca
     1921 tgccatttag taatggatag cccttaatct caatatttac cattcatatg taactttgaa
     1981 cgagatataa cgctgtttat ttagcgtatt ccccgttaag aaacctgcct gaattcctgc
     2041 gtttatattg cgataattag cggatatacc tgcttcttgt ttaaaataac gccccatttg
     2101 ctgctctatt tgtaaattat tgaaacgtgt tcttaatttc ccatggcttg catcaacaaa
     2161 atagctacct atttccggtt ttatttgcag gccatttagt tcaagcgttt tattaattga
     2221 cagaccagct tgaagagcga gaagatcaac agcgtttgtt tcgatttttg catctacaag
     2281 ttgattacct gctgaagata aatgatgata acgggcgcta aatgatggga ttatattcac
     2341 gtttgcagat tcccatgctt taccgatact aagccctgtt acaaatactg aacgatctaa
     2401 ttttactgta tttgcttgat aagttaagcg attgcttgct ttcccaaaac cggtatctat
     2461 cgcaacaaaa acaccatttt gccatgtttt cttcgcatac attgatagca ttgttaagcg
     2521 acctttaccg ctataataat ggtcaaaatt atttctcgct tttgattgag ataatatcat
     2581 accaagtgtg acctgattat ctaaggcttt ctctaatcct agatggataa cgttcgcatc
     2641 ttggttaaat ccatatccgg cagagcgatg ctctgtcgtt tgataatctg ttttagacca
     2701 tactttccaa ttttgggtcc cttccgaatg aattacatga tctaatgctg tagttacacc
     2761 taatacagca ttagtttgcg cagttaactc ggatgcgatc aaatgtgcat ttccattgta
     2821 tttaggtaat ggctcagcgg tttcacccga tcctttttct gaatgctcaa tgtctcctgt
     2881 gtattcaggt aacggatcag cagtttcacc cggccctttt tccgaatgct caatgtctcc
     2941 tgtgtattca ggtaacggat cagcagtttc acccggccct ttttcagaat actcaatatc
     3001 cccggtgtat tcaggtaatg ggtcagcggt ttcacttggc cctttttgag taattttagg
     3061 ttcttcctct ttctctggat tgaggtaata cacattccct tctttgagta aatggtagcg
     3121 atattcgcct gagtgcactt catttgctaa attaagagaa aagtcttctt catttgttgt
     3181 ttcaaataat ttaatctttt gggctgaatg ggcgctacca tttgtgctaa tttctatcga
     3241 aaattgaccg cttgcggtgt ctttaattgt gactatatcg ctcgctgttt tcgtcagatc
     3301 agcgacaaat ttaaatatgc cgttccctgt gagggtatta gcggtaagcg tattctgttt
     3361 ttgggtccct tccgaatgaa ttacatgatc taatactgta gttacaccta atacagcatt
     3421 agtttgcgca gttaactcgg atgcgatcaa atgtgcattt ccattgtatt taggtaatgg
     3481 ctcagcggtt tcacccgatc ctttttctga atgctcaatg tcgcctgtgt attcaggtaa
     3541 cggatcagca gtttcacccg gccctttttc agaatgctca atatccccgg tgtattcagg
     3601 taatgggtca gcggtttcac ttggcccttt ttgagtaatt ttaggttctt cctctttctc
     3661 tggattgagg taatacacat tcccttcttt gagtaaatgg tagcgatatt cgcctgagcg
     3721 cacttcattt gctaaattaa gagaaaagtc ttcttcattt gttgtttcaa ataatttaat
     3781 cttttgggct gaatgggcgc taccatttgt gctaatttct atcgaaaatt gaccgcttgc
     3841 ggtgtcttta attgtgacta tatcgctcgc tgttttcgtc agatcagcga caaatttaaa
     3901 gatgccgttc cctgtgaggg tattagcggt aagcgtattc tgttttgttg cagttggagt
     3961 gtttaaatgg atttggctac catttgcgac attcaagaca tctacaacag tgttggttaa
     4021 ggttgtccat accccagaaa gattaacggt tgaaccattg gcgccattaa atattcctgt
     4081 tgtactcaag ccggttaaat tagcaatact tccatcatta agttgaacct tagtggactc
     4141 ctgagctgta attgagccag ttaagtctgc tttactgcct aacgaaaatg ttgaattgtc
     4201 agctaaagag acattgccag ccactttggt tctctcccag ctgtttaaat cagtctctga
     4261 taaagtagcg ttaatattac aggttgctac gcctgtgcgg tcagagcgaa tacatgcttg
     4321 tgaggtacct tgggtaaaac caatttgagc ggtagcatta tctgttaaat taaaattacc
     4381 gttaatattg gcaacattac ggctgatttc aagcttgcca ttgtctttag ctgcaaagat
     4441 tgttgcgttg aacgatcggt tgatccagtc gccttcaatc aggacttctt tattcgctac
     4501 tttatcatag gcatgaggtg ttggacggcc cgataaaatg atttcgcttg taccttctgc
     4561 agaaagcgta ccgttgaggt tcgttccccc agtgataagc aatttgccgt ttgctacttc
     4621 attactgtat agaaccatta aaactaagaa ataaaagaat aataggaatt tggcggaatt
     4681 tttcgggaat aagtgaaaca gaagtattga aaacaggggc tttgcggtgc attgcccctt
     4741 tgttttatgt agaaaaaagt aaaaataatg aattgcgaaa aaatggctaa aactaaccgc
     4801 ttgtaccatt aaaacaacga aaaagtaaaa atgatttaag tagctttaaa tggcgtttaa
     4861 atcatctcgt gtccaatgta cacaacctgc ccgataacct caaaatctaa cgtttcatca
     4921 aaaatcacat caatagggct gtatagctct ttattatcgc tgattaagcg aataccgccc
     4981 ataatgcctt gcacacgttt aacccaaagc tgctcgcctt ggcggaacac ataaatttta
     5041 ccgtctttag gctgggtggt ggctcggttt accaatagca tatcgccatc actgatggtt
     5101 gggtacattg aatcacctga agcggtaata aatgcaagat agctcaaata gaagccacgc
     5161 acatctagcc atcgtttact tagcccaata tagtcatcag gggcatacac ctcgctattg
     5221 aaagcaccaa agcctgctga agcctgtaca ttataaaacg gcacacgttc catttcatct
     5281 gcagtttggg ctgccactaa tgttctaggc tcttctttta ctgccgtttg agcaaagcct
     5341 aactcttgct gcacgcttat tgggagagag ctgtagtggt attcaaaagc aacccccctt
     5401 tttccctcta tttgtctttt gagccaatta tttttggtag ctcttcttgt tacatttgat
     5461 ggttgttcag gtaaatttcc cattccagct acttcagatg ccgtaaacca ttcctttcta
     5521 attatagaat tcataaaacc ccttttgaat tcaagtggaa ttcaaaaaaa tttacaaaca
     5581 aattctcttg aaaatcaatt agttaattaa ataaaaccga aaaacactaa attcttttga
     5641 attctcttat tgaattcatt tggaattcgc tatattattc gttctgtagt taaatgattt
     5701 acatcattta agtagtttct aaaactaact ttcaaggatc tcacaaaatg gcaagaaata
     5761 aaagagttcg agatatgagt aatttcgaaa ttcgtggtga gctgatgaaa atcggtaagt
     5821 ccttatctca gttaggtatt gagaatggat tagcaaaaac cacagttcgt aatgcgttag
     5881 ataagccata tcccaaaggg gagcaaatta ttgcaaaagc gattggtaaa acaccgcagg
     5941 aaatttggcc ctctcgttac gaataaggag cttttatgaa ggaatggttt ttagccaaag
     6001 aaatcgcagg attatctgga gttccagaat taccgaatag cgtttcaaga ttggcaacca
     6061 aagaaggatg gcaaaaacga caaattcaag gtgtaagagg tgttacttac gaataccacc
     6121 tcaccagcct acccattgaa acccaacaac aacttagact aaacgccgcc cttgcggtaa
     6181 taccgcaagc ggcagaacta caacccaaac gagacgaccc cgcacttatc gcaaggctca
     6241 ataatgccac cgataaaggc agggataaag caaaaggcaa ggcagaagcc tgtatgcagt
     6301 tgcaagcctt tctcgaccaa gggtttagtt atacgcaggc agaagcggga gcggcaaccg
     6361 ctaaaaatgt gtcgcaaggt tctttaaaaa attggtacta caaagtgaaa ggtcaccccg
     6421 tccatttatg gcaggcgatc ttaatttccg aaagcggtag gagtaaaaag ccgcagctga
     6481 aagccaagat tactgaagaa gcgtgggact gctttttggc ggattacctc cgccctgaaa
     6541 agccggattt acgggcaagt taccgccgaa cccaagccat cgccaagcaa tacggctggc
     6601 aaatggcaag tttacagact ttccaacgcc gagtgttggc ggaagtgcct tacgaggtga
     6661 ttttgctcaa acgggaagga gcaaatgcgg ttgccaagtt agtgccggca ctacaacgca
     6721 cggtgaaaga tattctcgca ggagaatgga ttaacggcga tggctatcaa cacaatgtgt
     6781 ttgtgaaatg gcacaccggc gagattgtcc gccctaaaac gtggttttgg caagatgttc
     6841 gcacccgcaa gattttaggt tatcgaacat cgatttcgga gaatactgac agcatacgcc
     6901 acgcattaat ggatgtgatt tttaacgtag gcataccgaa aacgctcacg ctggataaca
     6961 cccgagcagc agcgaataag gcaatgaccg gtggtattga aaaccgctac cgctttaaac
     7021 ataccgagtt agacccgaaa ggcattatgc cgattttagg gattgatgtg cattttacct
     7081 caattcttta tggtgaagga cacgggcaag ccaagccgat tgaacgtgct ttcgggcgag
     7141 gcggaattgg cgagaaaata gataaacgcc ctgaattaag tggtttctat acgggcagaa
     7201 atgcacaaga aacccctgat aactataacg gcggtaaaga cggtgtggat tacaacacat
     7261 tcttgaaagc catagcagca gggatagaag agtacaactc tcaaaccgaa cgccataccg
     7321 aaatgtgccg aggagaattg agttttaacc aagtttggga gcgggattac cacccaagca
     7381 acgtaagaca agccagccct gaacagttgc ggttactgtt cttacaggca gaaacggtca
     7441 gcattaaacg caacggcagt tttacgctga aagcagccgg caaactttac ggcttaacta
     7501 acgtttactg ggcagaaagc ctgataggga taacggataa gaaagtagtg gcacggttcg
     7561 accccgatga cttacacggc aacgtgtatg tgtacaacct agaggggcaa ttcttagccg
     7621 aagcggtttg ccgagaggcg aaaggatttg gtgataccag tgcaagccgt gagcaaggtc
     7681 gcttatatca gaaagtagtg aaaagtgcca aagctcaagc cgaagccctt gaattactgg
     7741 aagctcacga actggcaagc ctcgccccac aagtggacgt gcctgagccg atagagaaga
     7801 aggtaaaaga ggtgctggtg aaagaagatt ttatcgtgga tttcaacact gtgcgtaaag
     7861 caacggtggt ggaagaaacc gaagagatca gtatttttga tgaagtgtta ctgaaacaag
     7921 agaaacaact tcgcatagtg gaataactag cggtcagttt ttgcaaaaaa tttgcaaatt
     7981 tttctaaaaa aacgaccgct tgaaatgaga tttaaacagg gtttaaacgc ctttaaggag
     8041 taaaaaatga cattaacagg gcaagcttac cgagtgattt tcaaggaact caaagagcaa
     8101 caagagagtg aatttgaaca ggctttcaag aaagggattg ccaaaattca gcagaatcaa
     8161 actcaggaat ccattctggt aaaccgtgat atgtttcaag ctgaaaagct aacactgctt
     8221 tcacaaaagt tggaagcggt tgagcaatta cttcgccttg aattaaatca gccgaatttt
     8281 caagcaaaac ttgaagacta cgctgacgca ttacgaacaa ccattttaag taaagtagcc
     8341 aaataagagc tttagactct tgaaggctta acatctgagc cacttttttt actgtcggca
     8401 ttataccgtc aagatcatca atagaagtta aatgatgaaa agaggaatgg tattcagcta
     8461 aacaaaaatt gattgtacta agtttaaaca aagtaatgca atttttcatc acgatggcat
     8521 ttgctcgttt taacttaaaa ccagagaagg ttgcacgagc agaatcaagg gctatttgat
     8581 gaatttcatc atctgttaaa gaacgaaaga gcataaaaac ctccaaacaa aaacaggaca
     8641 ccattatgac attaatcgac caaattaaac aacacatcgc cgaaagcggc agttctcagg
     8701 ggaaagttgc caaagaagcc gggattaatg ccggggcgtt atcggcttac ctgaatgaaa
     8761 attacaaagg caataatgaa gaacttgaag caaagctgat tgcttattta gagcgtattg
     8821 aggcgaagaa aagagagttt gtcgaagctc cgagttttat tgaaaccaaa accgcaaacc
     8881 aaattttcgg ttcgttacga tttgctcaaa atactggcgt gcttgccatt attcacggtg
     8941 cgagtggcgt gggtaaaaca caagcggcta gagagtatcg taaacgctat gcgaatgtat
     9001 ggttggttac cgcaagccct tctcgttcct cattaagtga ggtgttatat gaaattgctc
     9061 ttgaacttgg aatgaacgat gcaccacgcc gcaaagggac attggctcgt ttaattgttc
     9121 gcaaaatgaa aggcacagca ggtttattaa ttattgatga agcagaccat ttaccttacg
     9181 aagccttaga agaattaagg ctgttacaag aagaagtagt gaatgaaagt gacatcggag
     9241 ctggtgttgg cttagtgctg attggcaatg acaaagtcta cacccgaatg aaaggcggaa
     9301 ttaaccctgc tcacgaatat gggcgacttt ggtcacgcag tgccaaacgc accagtattc
     9361 agaaaaccaa aaaagaagat acccaagcgg tagccaaagc gtgggggttg agtgaaaacc
     9421 aagaagcact caaggtaatg caagccatta ctgaaactgg tggcggatta cggattctga
     9481 cacacacctt acgattagca gcgatggtag caaaagaccg aatgattccc ttaactgccg
     9541 atttaattca gctcgcacgt aaagatttac tctcaacagg agattaacaa tgagaacccc
     9601 aaaacccaaa cacagtttta accgcaccaa ccaagcagcg tgccgttttt taaaacaaac
     9661 ccaaaaggca attatccgct taaacagtct tggctttacg gtgctgaata ttgattttac
     9721 ccgtattaag ccccgcattg aggtggaaat cggcaataac aagcacattg ctcaaggctt
     9781 gatttatgag ggtaaagcct accgctacag cttcggtaaa agcgaagatt taggcagatg
     9841 ggaaggctac tacaccatgc tggaaggtat cagagtgtgg tggcgacaag cagcaattca
     9901 ctaggagaaa aacagatgaa aaccttagca ctattaaccg cccttttagg gcttgtaggg
     9961 tgtgatttag tccaaagcaa acaagctaaa aattacatcg gcaacaattt aactgaaatc
    10021 tgtatagata acgtggtgta cctgatttat tccggtgata aaaaaggcgg aattaccccg
    10081 aaagtaaata aagattttta cccttatacc tgtacaaaca aagaggaaag acaaaatggc
    10141 aaaacccgat gaaacaccca ttacaaaagc agaatgtcaa tctcagcttg cagagttggg
    10201 ggtgcaatat aaaaaactgc caatggcaat tacaagacat atttgtaatg ccacaaccaa
    10261 tatacacggc aaagttataa aggtcagtgt ggtagagaga gttggatatg gcgtccagat
    10321 taccgcacaa ggtaacgaga aatcttgctt agtaacctat gaagcaatgt taggtatggc
    10381 agaagcaatg ggattatttg atgaagttaa ggagcaaaat aatgactaaa aaaacaacca
    10441 gagtaaaaag tgcaacccaa agtgcgattt atcaaagccg agatgaagtg caagtggcta
    10501 taaaaatcat tggtgataaa cagcgtgaat tgcaacgttt agctacggca atgaatgatg
    10561 aattagctgc tattagtgcc agttatgctc aacataaccg ctaccaacat tatgccgaga
    10621 aagcggctga agcagaacgc aaaggcaact ataaggaagc tcaagaccat tgggaagttg
    10681 caaaattatc agcaaaaaag accgctaacc gagactgggc ggaacaacga gcggaatttt
    10741 gtaaacgtat gcacaataaa ccgttttagg gggaagtgat gacagaaacc gtaaaagcac
    10801 aacttaacag ccaattaaat gaggcaatta tccagttgat acaagctcaa aagtatttaa
    10861 accaagatga tgccattcgc agtggagtgt atatcggtac ggtgcaggat ttattgccaa
    10921 aggtgcattt aaagttatta acggcaaatc gtaaacattg atagagatga ggtaaaaatg
    10981 gctattacag aagaacaatg gaaagaaatt aagcaaaagc tagatagtat catagggaga
    11041 gttaaattta gatacaaaga gcatttactc acggtagatg ttgtacaaat taagcgatct
    11101 ttaaaattgg ctgtgtatgt tgatggggaa attgatggag cttggacaaa agaaggacac
    11161 gaaattcgcc cttatttaga agaagtttgg tatcgcaaag aacgcccttt ttttaatgct
    11221 aaggagaaga aagagtatag aggtttaatg agcaaaaaaa actgaatgaa aaaatagtaa
    11281 cctactcacc tatgtttcca tcaccaactg cactgactcg tcaatataag aaactagatg
    11341 gcttagagtt gattgaagtg atttaaaacc catttacagc ccatttaacc ccgagttatt
    11401 taaaggacaa caaaatgaat aaaaacgaat tttttaagaa ataccaccta gagggttcac
    11461 tagctatttt ggatttggat gcaaatgacg atcaaagcca attctgttac caagcattgt
    11521 tggagtatag agaactcccg ttagacacat ttcttgcaga gtactcagat gatgaggata
    11581 tgacagagtc agagtttatt gagttaatgc ttgatgaatt agagtggatt atccgcagag
    11641 ctaagaatgc taaacggttt gttaaaaaac gacaaaaaac gatgagggag gaaattatgt
    11701 aacagctcaa cccaaccaac gccacatcat ccgtctagct gagaaacagg tgtggcgttt
    11761 gttttaagtg ggcaatcaaa agcgataacg tttttaattg tttatttatc ggagtaacaa
    11821 atgacagaca aagccaagct aatccagctt atccatattg ccaagtcgca actagcaatg
    11881 gacgatttaa gctaccgtga aatgctgaaa cggctcacca ataaaaccag ctcgaccaaa
    11941 tgcacggtgg tcgagttgca caaagtctta cacgaactgc aaaccaaagg agcaaaagta
    12001 aaatattttg ccaaacgcag caaaaaaccg accgcttaca gcccggcaac aggggaaaca
    12061 gcggtaaaaa gccaaattgc tcataaaatc cgagcggtat ggataacaat gggcaaacac
    12121 ggatttttaa acgacccaag cgaaaaagca ttaaatgctt atatgcgtaa ggttatcaat
    12181 aataaaacta gaccaacaat attattaaat gtctctgcat tagaggctgg agatgcaagt
    12241 tatatgcttg aaatcctcaa aaaatggcat aaacgtgtga tgattaaagc cctaagcaaa
    12301 agttgcacag ttgatccaaa gataagctac gatgaattag tggagtggta caatgaagtt
    12361 atgtagatgc ccgatttgcc acagtgatat acacctagaa gctctcgtag aagatgaagc
    12421 cgggcgtgaa ctactcggca aaatcagcca attaacacac ggtgttgctc aaccgatggt
    12481 tgcttattta ggcttattta aaccgcaaaa aagtaacctt aataactcaa gagccttgaa
    12541 aatactcaat gatgtattag cactctaccc ttgctcattg ctactggctc aagccctatc
    12601 tgaaacggta gtaagcatac gcaaaaaacg tcagcaagca atggaaaacg ggcagaaaat
    12661 tgaaccgcta ccgaaccata actatttaaa atcggtgtat gaaacgcaaa aagtccattt
    12721 tgctgtggtg agaaccggca aaaccgaacg cgaaacaatg aaagccgaac tggaaaaaac
    12781 acgcaatgcg attgcctatg tgcaacggtt tgttgattta ggacgtgaag aggttgttaa
    12841 aaatagccct gagtatcaga tttggttagc tcataaaccc aaataacaca agccaccaaa
    12901 aggtggcttt ttaacttttt gaaagtgcga taaaacctga tttaaacagg ctttaaacgg
    12961 gttttaagat tgagtttaaa aaaatatttc acaaaataag aatggttttt tatacaataa
    13021 ccgaaacggt ttttggatag gaaatagaca atggaattag tggatatttt tgaagaaaaa
    13081 gccccggaag tgttaaccga tttagccaaa catattgaag tcgcattaga agaaaaatgc
    13141 caatttagcc ccgaaaaggc aaagcaaatc ggtattgata ttgcccaaca aatggctcaa
    13201 aactggggtg gcgaggtaat ttatattcct cgcaatttgt tgatggctct taatgagcga
    13261 gatatgaaaa tttacgaaga gtttaacggt aacaatcacc gtgaattggc tcgtaagtac
    13321 aatgtatcaa tgcagtgggt atataaaatt gtgaagaaag tccataagga cgaagttgcc
    13381 cgcaggcaga tgagtatgtt tgagatgtaa atcatttttt cccagccctg atcattttca
    13441 gggctttttg tgtattaaaa gaacgcagtc tttaaacccg tttaaaatca atttttcagc
    13501 aaattcttta aactcctttt aaagcaattt taataggagt gtgctatgtc tcaaaatctt
    13561 acctttcgcc aaatttttga ccgtttaatc ggacacgagg gcggctatgt caatcaccca
    13621 caagacccgg gcggagaaac ccactgggga attaccaaac gtaccgcggt tgctaatggt
    13681 tatatgtatg caatgaagaa tatgacccgt gagcaagcct accaaatcta cgaaaaagcc
    13741 ttctggcaac gttatcgttg tgcagagctt aagccagcag ttgcttatca atttttcgac
    13801 gcagtggtta atcacggttt tggtaatgct agtcgtatgt tacagcgtgc agtaggcgta
    13861 gcggatgatg gcattattgg caatattacc ctgcaggcaa ttaatgcaaa atctgaaagc
    13921 gatctcttaa tgctatttaa tgccgaacgt ctgaagtttt acacaaaatt acgcaacttc
    13981 cccacttttg gtaagggttg ggtcaatcgt gtggcaggca atttagaata cgcagcacaa
    14041 gataatgagg tttaaaaatg gcaaaactat ttaaagaact actctcaacc aatggcacat
    14101 tatccaccac tagcaccgtg cagttttttg gcacattgtg tgtatttggt ttaatggttt
    14161 atgcagtaat tacccaacaa ccttatgccg aaagcctatt aaataatgtg ttgatttatc
    14221 tttttggggc aactaccgct aagggtgtag taaccagcta tcaagccaaa attaaagggg
    14281 gtatccataa acaacaaagt acatttaaag aggaggaggg atgttaatgc aacaaatttt
    14341 aggcgtgatt ggcgtcatcg cagcaatatt gtcttatgct ttgtttaaaa gctggcaact
    14401 aaaacgtgag cgtaaaaaga atgccatatt acaagctgaa aaacagcaac aagcggttga
    14461 aattgagcaa aaaaaagcag aggtaaaata tgtgtatata tcaaagaaaa acaatgaaaa
    14521 agttactcgc agctctgcta gcgatattga tgacaagttg cagcaacacg gttacttccg
    14581 tgacaacaac aggttgcacg gcgttcagtc tgatttatcc gaaccgtgcg gatacgctgg
    14641 aaacgaaacg tcagattctt gctcataatc tgacttttga ggaaatttgc caaaatgaaa
    14701 tacattaaaa aattacgtaa acgctggcaa atttggcgtt ttttaaaaaa tcatcctgag
    14761 gttgcacaac gccgagattt attagcgatt gccattaaac agggcgtaga aaaccctgta
    14821 gggcgtgcaa gacggaggga gccataatgg atttatttac agtcttttgg gagtctatcc
    14881 gtgctaactt tggttttatt tctgcgttac tcaccattgt tgttacggca ttttggctca
    14941 agctagatag caaatacgcc aagaaaaatg atcttggtga attgctggaa attgccaaaa
    15001 gccacgataa ccgcttaacc accttagaaa ccaaggtaga aaacctgcca agtgcggtgg
    15061 acgtggaaaa gctgaaaacc ttagtcactg atgtaaaagg cgataccaaa gccacctcaa
    15121 agcaggtgga tagtatcagc caccaactcg gcttgttatt agaagcaaaa ttaaaggaat
    15181 gatatgtcgt taagagattt actcactcaa gaccaacggc tggtgatttt acgctcatta
    15241 gcggaagccg gctatgatgc caatgagtca attattagtg attgcctaga tttatacggt
    15301 cacgatatta gccgtgattt agttcgcacc catttaagtt ggcttgaaga gcaaggctta
    15361 cttaccttag aacgtttgcg agatggctat atggtggcaa gcattaccca gcgtggctta
    15421 gatgttgctc aagggcgtgt tgtggtaagt ggcgttaaac gccctcgccc taaaatttaa
    15481 acggtattta aggagcgttt aaatgagcga gaaaaatact cgtggtcgag catcaaaagt
    15541 cgatttactg ccgccgaata ttaaaaccca actggcgatg atgttgcgtg ataagcagta
    15601 ctctcagtca caaattttag aagaaattaa tgacttaatt cgggattgtg gcttgcctga
    15661 tgaaatgctg ttaagcaaaa ccggattaaa ccgctacgca agccgtatgg aacgcttagg
    15721 ggcaaaaatc cgtaatgccc gggaagttgc cgaaatttgg actaagcaat tcggcgaaat
    15781 gccacaaacc gatattggca aaaccctaat ggaaatggtc aagcaaattg cctttgaaac
    15841 ctcattgaaa ttaggcgaac aagaaggcgg tattgagcca aaacagcttg ctttgctctc
    15901 gtctgctatt caacgtttag aacaagccga aagtttgagt tttaaacggg aacaggcaat
    15961 ccgtaaagag gtcgcacaac aagccgctga aaccgctgaa aaagtggtgg tgcaagcggg
    16021 tttatccgct gaaaccgtcc gtacgattaa agagcaaatt ttaggaattg cataatggca
    16081 ctactcaata acagaccgct gaatgaatta gcccctgaat gccgttcgtt tttagagagc
    16141 attcacgctt ttaatcctaa cgaactgtta ttgggctatc aaaagcggtg gattgcggac
    16201 gacagccaac tcaaaattgc cgaaaaaacc cgccgttgcg gtttaacgtg ggcagaagca
    16261 gcagataacg ccttaatcgc aagcacccgc aaatcagacg gtggctctga tgtattttac
    16321 attggctcaa ataaagagat ggcacgtgaa tatatcgatg ctgtcgcaat gtgggcaaaa
    16381 gcctttaatc gtgtggctgg agaaatccaa gaagagatct ttgaagacga agataaagac
    16441 atcttaacct atgtaatcta cttctcatcg ggttttaagg tcaaggcatt atccagtaat
    16501 ccgaaaaacc tgcgtggtat gcagggcgtg gtggtgattg atgaggcggc attccacgaa
    16561 tacttagcgg aagtcttgaa ggcggcgtta gctctcacaa tgtggggggc gaaagtccgt
    16621 attatttcga cccacaacgg ctcggaaaat ctgtttaatg aactgattac cgatagccgt
    16681 gccggccgca aacgctactc tgtgcatacg attacgattg aagatgcctg ccgtgatggg
    16741 ctatatcaac gtatctgcca agtagtgacg aaaaaaacgt ggtcaccgga agcagaaaaa
    16801 gagtggattg aaaacctact caacgacacc gccagcgaag aagacgcatt ggaagaatac
    16861 ttctgcgtac cgaagttcgg ctcaggctta tggctctctc gtgctttgat tgaacggcag
    16921 atgagcgaag ccacgcctgt tatccgcttt acggcaaaag atggtttcag ccttgtggct
    16981 gaaccgacac gctatgcgga gattgccgaa tggtgtgaat tagaacttga gccgatattg
    17041 gcaaccctta accctgattt gttgcatttt atgggcgaag actttgcacg cagcggcgat
    17101 atgacctctc tggtggtgct tgctcagcaa caaaaccttg ttcgtaaagt cgcctttatt
    17161 gttgagcttg gcaatatgcc ctacaagcaa caagagcaaa tcgtgatgtt tatgcttaaa
    17221 cgcttaccgc attttgccgg tggggctttt gacgcccgag gcaatggtgg ctatttagcc
    17281 gaagctgcac gagatgaatt tagcaccttg attgactgcg tgcagttatc ggaaaaatgg
    17341 tatcgggaac acaccgcccc ctttaaagcg gcattggaag acggcgagct ggaagctatt
    17401 ccaaaagatg cggatatttt ggctgatttg cgttcatttc aggtggtgaa aggcgtacca
    17461 cgcataccgg ataaacgcac taaaagtgca gatgggaaaa gcaaacgaca cggtgatacc
    17521 gcaatttctt tgctgttggc tcattatgcc agccgacaat tagtgcaaat gccagttgag
    17581 ccggtatcac gccgtccacg ccatagccaa caattaacag aaggatatta acaaatgaga
    17641 tatgtaccac tttgtttaat gatgtgtgcc tcattctacc tatttattaa caacattgac
    17701 ggctggggtt ggggaattct tttatctttt attttagcga gtgcagctta tggcaaagaa
    17761 gaataattta attagtgaaa ttgccacccg tgccagaagt attgattact gggcatttgg
    17821 ctattatctg ccgaaccctg acccgatttt aaaacgaatg gggaaagata tttccgttta
    17881 ccgtgagttg ttatcagacg ggcaagttcg ctcaggcgta cgccgtcgaa aagcggcgat
    17941 taaaggctta gagtggcgaa ttaccaccac taacaatgag aaagtggacg agcagattta
    18001 tcaaatattt aaccgcttgc cgatgaataa cctgattacc gaaatgctca atgcagcact
    18061 ctatggctac catatcagcg aggttatttg ggcaaaagag ggtgagttat ttgtgccaac
    18121 agaaatcatt ggtaaaaagc ctgaatggtt tatgtttgac gacgaaaacc tactgcgttt
    18181 ccgtaccaaa gaaaactggg cggacggcga attattacca gatgataagt tcttgcttac
    18241 tacgcaagaa gccacccaag ataatcccta cggtttaggc gatttatccc tctgcttttg
    18301 ggcggcgacc tttaaaaaag gcggtttcaa atactggttg gaatttaccg aaaaatacgg
    18361 ctcaccgtgg ttgattggca aacaccctcg ccaagctcac aatacagata aagagcgact
    18421 agctgatgcc ctagagggaa tgattagctc agcgattgcg gttatccctg aagatagttc
    18481 ggtcgaaatc aaagatgcag cgggcaaatc tgccagcacc gatgcctttg aaaagttcct
    18541 caatttttgc aaggcagaga tcaatatcgc cctacttggg caaaaccaaa ccaccgagca
    18601 agaaagcaac cgtgccagtg cacaagtagg cttggaagtt atggaagata ttcgctcgga
    18661 cgaccaagcg atgattgaag ccaccttcaa caccttgttg cagtggattg tgaaatataa
    18721 cttcaatgtg gaacaactgc cgaagtttga attttacgag caagaagcca tcaacaccga
    18781 ccaagttgaa cgagacagta aactccacgc aatgggcgtg cgtttttcca aagcctactt
    18841 tgagcgtgaa tatggctttg aggcgggtga tattgaggtg caagcggtcg aaaaagcgga
    18901 aaaatctgca aataccacag aatttagcga aggacaacgc cgaacccaag tgccaatgcc
    18961 tgtgccaatt ttggaacagc tagaacacga gggagaagca atggtggacg attggctaca
    19021 aggggtgaga gataaactcg ctcaagcaga tagtctggag gatttccaaa gtcagctaga
    19081 tagccttatc cctgaattaa gctttgccga atatggcgag ctgttggcgt taggctcaac
    19141 ggtggcagag cttgcaggca gacaatcggt aaacgatgaa attaaggcaa aacgcaatgc
    19201 gtaaatttac ctttaaacag caagcccaat attttgagaa aaaactcaat ctccgcacga
    19261 atagctacct tgatattctg ggcgaagagc acgactattt tttcgtggtg gctggggcga
    19321 atcgtaatga agtgttgagt gagtttcgca aggcggtgga tgaggctatt cacaacggcg
    19381 aaaccttaga gaactttcgc aagcggtttg atgaggttgt ggcaaaaaca ggctgggagt
    19441 acaacggcgg acgaaattgg cgaacacgga ttatttacga taccaatgtt tacggggcgt
    19501 ataaccgtgg acgtttgcaa caacatttag agcttgcgga ggatatgcct tattgggaat
    19561 atcaacacaa cgacaacgcc caccctcgcc cacagcacgt ggcgtgggac ggcttggtgc
    19621 tgcgttatga cgatccgttt tggaaatatc actatccaat aaaagcctac ggttgccact
    19681 gcaccgtgca agcgttagat gattttgacc tcaagcacga tggtaaaacc gtcagcccac
    19741 cacctgaaat tgagtttgaa caaaaaacgg taggcgtgcg gacaggcaat tcacgagtcg
    19801 taactgtgcc gaaaggggtt gatgtgggtt ttacaccgca taattttgat aatctcaaag
    19861 ccgggagaac agcaaatgta gataagttgc taatgcaaaa aatggttacc gccgatccta
    19921 tatttgcctc caaagcagtc agtgatttat tggcaaaaga gccggttgca aagttacata
    19981 accaagcctt taaaaattgg gtaaatgatg ttgccaatgc taaaggaagt gcattatccc
    20041 aagtagagaa tatgcagttg ttaggggtac tacctgttgc ggtgattgaa cggttacagc
    20101 agatcaattt agctccgcaa tctgcaatta ttgcaataac aaaaggcgat atggtacacg
    20161 ctctacgaga gctaaaacaa aataccggta ttgccgtacc gttggaattt tgggaaaagt
    20221 tgcctgcaaa gttacaatac ccaaatgcca tcttgttgga aaaacaacaa gcagaaacta
    20281 cattgctctt tatctatgac gtagctcaaa ccaaaattgc cgtaaaagtc aattatgatg
    20341 caactttaca agatagacac ggagcaaaag gacggcagaa agtcttactg aatcgagcgg
    20401 ttacaggtag tttattccaa gatacaactg gattgaaaag ttcaaagtat gaagtgctat
    20461 tgggagaaat gtagcggcga aggactcgaa ccttataatg taggttaaac ctacaacctt
    20521 taccaattag gaaactaccg ctacattgtg taaattcact atacccctaa attatttttt
    20581 aatcaatagg agaaacaaaa tggcagaaaa acaactttcc tttaaagtag ccgaaatagt
    20641 gccaatgtca gctattaaca ctatgcggca cttaattagt ttggcattac aagcgaatta
    20701 tgagagagaa aaatctcgtg cttgataatt tcaatggctt gtagttcggg aactatatta
    20761 tctgaaacaa gccattgttt tgttgatgtc agacatttag cgatgaaatc cataattaaa
    20821 ccattagcag tagctcgttg ttccggagta agttgtaacc acgacgcctc tgtttgttgg
    20881 tctaatgtat aaagaaacat aacggcttct ttttctgata atgggtcaag tggtaatttt
    20941 tcagcttttg tgactaacca ttgacgggaa agcaataaat cataaaccag ttctattgct
    21001 tgttctgcat ttaccatcat attctaattc cttaatatat cagggcaaca ttacccaatc
    21061 tatattttac tgagatttgc ttaatgatca aaatcaccct caacaacacc caagcggtca
    21121 aatcgttaca acaaattgca aatcagctgg aacaacctcg ccgactgtat ggcattttag
    21181 gggaaaccct aaagaaaatt cacactgcac gctttaaagc ggaacaagac cccgatggca
    21241 acaattggaa accgttagcc agtagcacgc ttgctttaaa acgtaaacga ggtaagtcca
    21301 ctaaaatctt acggcaagac ggctaccttg ccgataaaac ggcttacaat gttacaagtg
    21361 ataatgttga gtttggctcg caggaagtgt atgcacggtt gcatcaattc ggcggtaaag
    21421 ccggacgagg caaaaaagtg actgtaccgg ctcgcccgtg gttaggcgta ggcgataaag
    21481 atgagcagtt acttttacgc aaagctgagt ggcatttagg gcaaattatc ggtcgtatgg
    21541 gaaaataacc gcttaaaatc aaatttaacg cataaaaacg cctctgtgcg tttttatttt
    21601 ttattcgcct gattattcga ctcaaaaaat ttaaacgctg ttaaacgcat ttaaacggta
    21661 tttaaacggt attgccgatt gtgataaatc ctctttatta aaacaccctc gaaaaatctt
    21721 taaaccagtt taaaagctaa tttctctcca aatccttatt attgccccat cacggaggca
    21781 atatgaccca aattgaaatt tttaaagccg gtaagcgtta tgacgccaac ggcacgttaa
    21841 ttgacattac ccctgaaatg ttgcaacaga ccgttgctgc ctataacccc gaatttcacg
    21901 aagctccgct ggtgatcgga cacccgaaat ccaacaaccc tgcgtggggg tgggtaaaag
    21961 ccctttccct tgagggcgat gtactaaaag cagaagtaga tcagctagac gctgagtttt
    22021 cagaaatggt ggcaagcggc aaattcaaaa aagtctcggc tgctttttac ttacctgata
    22081 gcccgaataa cccacataaa ggcgtgttat cgctacgcca cgtggggttt ttaggggcaa
    22141 tgccaccggc ggttaaaggc ttaaagcagg tggaatttgc agaagatgat gattttgtcg
    22201 tcttttctga atggggggtt gcaagtctat ttagtcgttt tcgtgattgg ttggtgagtg
    22261 agttcgggct tgaaaaagcc gataaagctc ttcccccaca cgaaattgat tggctgaaag
    22321 aagaggcaat gcgaagccaa attagcaaac aggtacacgc cgaacaggca acacctgaac
    22381 cgatttttaa tgaacccgca caaccagaag gagaacctga aatgagtgca gaagagaagg
    22441 aagagcttga acgcttaaaa gcggaaaacg agcagttgaa agccgaaaaa gcccaagccg
    22501 aagcagagaa aatggaagca gagctggaag ctgaaaaagc tggtaatgca gagttttgtg
    22561 aaggtttagt caagcaaggc aaacttgccc cggtggcaaa agcggcgttt gtgcaggctt
    22621 taaataacct ttcagtatta aaagccggtc gtgagccgga atttaatgag ggcgaagatg
    22681 tggtttcaca gtttaagtct gcaatgtcgc aatcaccaca gattatccaa tttggagaat
    22741 atgccacagc agataaagcc aacccacaag aagcggaagc agtggaatat gctgaaggca
    22801 cagatccagt ttcgattgaa gccgaccaaa aagtgcgagc ttatatgaaa gcccacaatg
    22861 taggctacgc tgcggcattt aatgcgattt atcgctaatg tttaacccac aactgaaaag
    22921 gaatactaat gacaactcat aacttacaaa aactgcgtgt acaagacccc gttttaaccg
    22981 agctggcaca aggctatcac aataacgagc tggttgccga gaccttaatg ccgactgtcg
    23041 aaatcgaaaa ggaagcaggc aaaatctcaa ctttcgggcg tttagctttc cgattgcctt
    23101 ctaccgtgcg taacttgcgt ggttcatcta accgtttaga tccagaagat attggggcga
    23161 ttgatgtggc gttagaagaa cacgatgtgg aatatgccat tgattatcgt gaagagaacg
    23221 aagccatttt ttctttacgt caatttgcct taaataccac ccaagatgtg attgcgttag
    23281 gtcgtgaaaa agcggttgca accctcgcct tagatgaaag ccgttatgaa acagacaata
    23341 aagtcacgct gtccggcacc agtcaattca gccataaaga tgctgatgtg tttaaggtat
    23401 ttgataccgc tattcgtgcc gtgaagcggg ctatcggtcg taaaccgaat gtgtgtgtga
    23461 tttccggtga tgtgtgggca gcgttaaaag aacatccggc agtggttgaa aagctcaaat
    23521 acagtcaagt agcgattgtt acccctgagg tgtttgccaa actgatcggc attgagaccg
    23581 taaaaatcgg tgaagcggtg tatgaagaaa gcggtcagct caaagatatt tggagcaaag
    23641 atattgtggt ggcgtatgtt gctcctcgtt cgaccggagg caaaggcacg gtttacgagc
    23701 cgtcttatgg ttacacggtt cgccgtcaaa aaggcttatt tgttgatact tataaagaga
    23761 gtggcggcaa aattgaagtg gtacgcacga ccgatattca caaacctcat ttagtgggtg
    23821 cggcagcagg ctatttaatt aaagctgccg tataagccac ataccatagg gtgcgtttac
    23881 taacgcacca ttatctcaaa cacattacat agaggaaaaa acgatggcta aatttattgt
    23941 tttacatacc ccaattttac acgatggcaa acgctatgag gtgggggcag aaattgaact
    24001 caccgctgaa caagctgaga acaatgcgat caacttaaaa gcggtagaag ctgttccaaa
    24061 accacctaaa aatgcctcaa aacctgaaga tgagccaaaa cccgaaggtg aacctaagcc
    24121 tgaagatgca gtgacggaag atgatgtgaa gctggctgaa gcaaaaattg cagaagcaga
    24181 gcaaccaaaa gggaataaaa aataatgtat gtgcagattg atgagctact cacggcgttt
    24241 agtcgcaaga ttgtggtgca gctcagtaat gatgaaccga cagcgaccga gcctgattta
    24301 aagatgcttg aaacggcagt taaagtggca aatgagcgaa ttgatgcagc gttacgctct
    24361 cgctatacct tgccgctggc tgatgtgcct acgcttatta atcagcacgc attaaccctt
    24421 gcccgttatt ggctttatgc ccgccgtcct gaaaccaaaa tgccggacac cgtgaaggaa
    24481 acctaccaac aagcggtgaa agagttggaa cagattgcaa caggcaaact gcatttaggc
    24541 attgctgaaa gcagtcaaat tttagaagac gatgttttag ccgataacag tgaatatgaa
    24601 gttcgggcga acgctcgcat tgataccagt cgttattaaa gattatgtca gccacacaac
    24661 cgattttaga agatatttgc caacacttgc ggcaagcatt gcccgagtgg gaggtggaac
    24721 tcttcccgaa tgacccaagc acctatcatc tctcgcacat taacggtgcg gtgttgatta
    24781 gctatttggc aagcaaattt gagaaacctc gcactacaca agcggtgctg caggttcgcc
    24841 acgttcagat tgctttaacg gtgctgaccc gtgatttgca taatgatgtc ggggcgttgg
    24901 atttgctgga taaattgcgg ttattggttg tggggtttaa gcctcaaaac tgcggtgagt
    24961 gttggttgat tgatgaattt tttaatggct caagcgaaga aaccgggctt tggcaatatc
    25021 agttgatttt gcaaaccgaa actcagcagg tgcaacacca agcggtcgaa aatctgccaa
    25081 aatttgtaac cgctcatctt cgccgtcaag accagcctgg ttcgcccgga cttaaaactt
    25141 aaatcgtagg gtgcgtttgc taacgcacca tcaacatcac ctgattttac ggtgcgttgg
    25201 caaacgcacc ctacaaggag aatattatga cgtttcacca cggttctgaa accgaacgag
    25261 taaacggtgg ctctgtgccg gtatcggtgg ttgatagtgc cattatcggc attgtcggta
    25321 ctgccccaat cggcgcagtt aatgaattga ccgtctgttt aaccaaaaaa gattttgcca
    25381 aattcggcac gattttaaac caaggcttca ccttacctga tgcctttgat attttagctc
    25441 gttacgcagc gggtaaagtg tatgtggtga atgtgcttga cccttcccgc caccgtacta
    25501 ccgtgaccga tgaagtcctc acactggatc gcaatacgct gaccgctcaa acagcgaaaa
    25561 ccggcattat tgcggtgacg agagtgaaag acggtgcaaa cgtgctgaat gctgatcggt
    25621 attcggtcga tctgcaaacc ggcgtggtga cctttgtctc tgcaccgtct gccccgaaaa
    25681 ttagctatat ctatgctgac ccgagcaaag tgaccgaaga ggacattaaa ggcggggtgg
    25741 atagcttaac cggtaaacgc aaaggttttg aactattgcg tgatggtttt aacctttacg
    25801 gtgcggatgc caaaatctta atctgtccgg aatttgataa aaccgcaagc tgtgccgctg
    25861 cccttgccac ccttgccgac caactgcacg ctaaggcgta tattcaactg ccgaaaggga
    25921 cgagcttatc aaaagctatt caaggacgtg gcccggcagg gacgattaac gcctcggcaa
    25981 gcaatgagaa cgtacgccat ttcttcccgt atgcgttggg gtcaagcaat actcttgaaa
    26041 gccttgcgac ccacgccgcc ggtttgcgta tgttgattga tgtggagaag ggctactggt
    26101 tcagcacttc caataaagag ctgaaagggg tgatcgggtt ggaaattccg ctgacggctc
    26161 gggtggacga taagcaaagc gaaactaatc tgctcaatgc ggtagggatt accaccattt
    26221 tcaatagttt tggcacaggc ttccgcttat ggggcaaccg ttcgtctaac ttcccgactg
    26281 tgacccatat cagcaacttt gaagtcgcct ctcgcacagg cgatattatt gatgaaagca
    26341 ttcgccaagc cgaattgcag tttattgatt taccgattga tgatgcgttg ctggatagct
    26401 tccttgaaac catcgacact ttcctgcgta cgcagaaatc gttggtaggt tattcggtga
    26461 gcttagacta tgaatatgac ttggttgatg cattcagccg tgggcaagtc ccgattgtgt
    26521 atgaatacac tcctaaaatt ccgggtgaac gtatcagcaa taaatctgtg atgacccgta
    26581 aatacttggc aaacttggtg tcgcaacgat aaggggaaat aaatgagtat tgcaattaac
    26641 caaattgtaa acggaaatgt ttacattaac ggcaacagcc aaatggggcg agtgaatgag
    26701 gtcaaaatcc ccgatattga gtttgaattt atcgaacaca aaggccttgg aatgcacggt
    26761 gtgattaaat tacctgccgg cacgaatgcg attgaagcag aaatcacttg ggacagtttt
    26821 tatccggaag tgcgtgcggt aatgcttaat ccgtataaat acactcagtt gcaaatccgc
    26881 tctaacctgc aagtgtttaa ctcgcagggc ttggcagccg aagagccgat ggtcaccatt
    26941 atgaatgtgt ctgccggtaa aatcggcggc acagggcata aaaacaaaga aaatgccgaa
    27001 tttgccgaca cgttaaatgt acattcaatt aaacagaccg ttgccggtcg tgagctgtta
    27061 tttattgatg tgctggcgaa tatctaccgt gtgaacgggg tggatgtgtt gcaaaaattc
    27121 cgcaccaata tcggttagta aaatctttaa agccgtttaa aatcaattta aacaccgttt
    27181 aagtaaactc ctttgtgaag ttaaacaaca accactcaca aaggagtttt tttatggcta
    27241 acgcagctca acaagtatta accaatcttc gtgtacaaac cacttataaa ttgcaatacc
    27301 ctatcgcttt accagatggt agagttattg aaaccttaga tgtacgtcgt ccaaaaggta
    27361 aagattttcg tttgcttgat gaaaaagggt ttgaccctga aaaagatggc gtaaaaattc
    27421 tacgcttcta tattcaacag ctcaccacac ttgtgccgga agatattgat gaactcgatg
    27481 ctgccgatat taagggctta aataacctat tggaagatat gcttattgag ggaaagtccg
    27541 acaaataagt gaactagata ccgtttatgc tgatctcgct tggtggttta agttttcacc
    27601 aagcgagctt ttagaattgg atttagtgga tattccgaaa tgggtagaac agatgaacag
    27661 gcaaataaaa gccggttatg ggcagatttt acggtagagc caaataaagg gttcggtggc
    27721 taaagtaagt aaaatggcga cgccccaagc tagatacaca ccaacccacg ccaagattgt
    27781 caaaatcaat ccataaatga gtacaaaact aacgccctcg cttaggcttt gtatagattg
    27841 aatcgcatct aaccagtaga agatactcca accgactaat gctaccgcta acaaccattt
    27901 agcggtgcat tctgttcttg ttacacaagc ctcgaaacca gcccactcag taattcgatt
    27961 atttaattta gtcagcatac accacctcct taaactttcc ctattttacg ctaaggattt
    28021 aaccaatggc aaataattta gctatcggaa tggtaattgg tgcaagccta tcatcaggtt
    28081 ttcacggtgc tttttcagga gcaaagaaaa agctagaaca attagaacag gctgtcgcta
    28141 aaaatcagca aagccattct aaattaggtt ctgagcttac tgcattgcgt agtaaacaag
    28201 ctactctcta tgcagaaatg agccgagcca gtagtaaagg tggtgctggg cttgctctta
    28261 tgcaacagga atataaaaaa attggcaaag aaattgccaa tattagccga gagcaacgca
    28321 aatggagtac agaattagaa aaatctattg ctaagcaaac taaattacgc aatgcaatgg
    28381 aacgtcatac acaagctaaa gaaaaccgag acgagcttaa agggaaaata attggaacgg
    28441 tagcttctgc ggcaatgggt gtcggcgtga tgaaaaccta tatggagcaa gaagaagctg
    28501 cgaataatct taaaatttca atgctgaaag cagatggttc tttcgggaaa tttgaagaga
    28561 ttggcaagat tgctgacaca ttagggacag atttaccggg aacaaaaaaa gatttctaca
    28621 agctcgcaat ggcattaaag aaacaaggtg tttctgatga tgtgctgacc ggaggggcat
    28681 taaaaacttc tgccgaactg aatgtcttgc ttgatatgga tcaggaaagt ggtggagagt
    28741 ttttagcgaa gtttatggaa tctcaccggc tcaatgaaaa tgagttacca caagcagccg
    28801 attatctgca acgagcaatg tttgccggcg gtttaagtaa agagcagatg tatgaatcga
    28861 tgaaatacta tgctccaaaa ctgaactcaa tgaaacttac aggggctgag aataccgaaa
    28921 aagtgctggc gattgaagca atggcaggtc agcaaggctt agagggttca acctttggta
    28981 cgggcttaaa tatgatgtta agccgtatga ataaaggacc ggaaatgctc aaggcagcag
    29041 ccaaaggaat gaaagccgaa gctcgagaga tgatggaggc tgtcggagta gaatttaatt
    29101 tctgggataa aaacggctca ttcaaaggca ttgatggaat gctggctgaa atgacgaaat
    29161 ttgaaaaaat ccgcaaaaaa ttcggtgaca aaggtgtagg tttagttgca gaagagctgt
    29221 ttggcattga aggtgggcgt cttgctgata ttttggctat gaaaggtcaa aaaggcttag
    29281 atgaaatgct gagtaaaatg cgtgaacaag ccagccttca agatcgcatt aaccttaaaa
    29341 caaatacatt aggtgctgcc ctagaatctt taggaggagc gtgggaaacc tttgtggggg
    29401 atattggctc tgtctttgcc gaagatataa aagggttttc aaaaggcttg caatctgcgg
    29461 tggaatggct tgtgccattt gtatcaaaac ataaagaggc gattaaattt gtaggtggtt
    29521 tggttgctgg acttgttatt ttacgaggcg ttttacaagc tggttctttt gttattcaaa
    29581 cggtaactat ggcaatgaac gccctcaaaa ttgcagcaat gaccaatcct attgtcttag
    29641 tcattggagc aatcgcagcg ggtgcttact tgatttatag ccattgggat agcattgccc
    29701 cttggtttgc aaatctttgg caaaatgtga ccgcttactt caatcaattt tggacttggg
    29761 taaaaggtgt ttggtcgggc gtgacggact gggtgtcttc tgtgtggaat ggtgtagcca
    29821 cttttttcgg gcagttatgg actacgatta ctagcttatt tagtgtagct tggacaaata
    29881 tccaaacaat atggggagga gcaacagctt ggttctctgg tttatgggat gggattaagt
    29941 ctgcggcttt tggtatgttc gacagtatta gccaaaaagt cagctctgtt gtagcaagca
    30001 ttaagagctt tttaggcttt ggcgatgatg tggataaaat gggggcaaaa attggcaatg
    30061 tagctaccca agtcatcagc aaagaacact caggacaact cagcaaaacc gcagatatgg
    30121 cagccaatac agggttctct agcggtggtt tcaccgggca aggcggtaaa tatacccctg
    30181 ccggtattgt gcataagggc gaatatgtga tgacgaaaga agcgacctct cgtttgggtg
    30241 ttggaatgct ggatagctta aactatggcg gtgctatggg tttcactcaa cctgttttgc
    30301 aaccgatttt aggtaagcaa aaaggctttt ttggctcact ttgggacgat gtgaaattcg
    30361 gggcaaactt tgtcggcaat ctgcttggct taaatcaagg cagcctgaaa acgcctgatt
    30421 ttaacccaaa tgcgaccaat gcaagcggtc aaaattcgtc gatttttagc aattatgaac
    30481 cactcaaccg aaatgcggta atacagcagg aaagctcgca agcaccgcag atagtcgtga
    30541 actttaaccc gacaatcaat gtaaacggta atgctacgca agaggctcaa tcgcaaatta
    30601 tgcagcagtt gcaaatgagt gcttatgagt ttgagaaatt gctcaatcgt gtgctagacc
    30661 aacgaagccg tagagcctat taggagtgat ttatgtattt tatgttaggc aatattgcct
    30721 ttgagccggt gaatttaact gaatttagcg agcaacatac cgccgaattt gccgaacacg
    30781 ctgtgctaaa aggcaaacct cgtttacaag cgatgggcga aaagctaaat gaacttaatt
    30841 tttctcttcg gttgcaccat accataggcg gtgtagagag ccgttatcaa gccttgcttg
    30901 aagccaaagc gaagcaagcg gcattggcgt taatttgggg cagtcgttat aagggagatt
    30961 atgtaattgt cgatatttca tcgaccacat tgttcaccga tggcagaggc aatgccctcg
    31021 ctcgtgaaat gcaaatcagc ctgaaagagt ttgtgggcga taatgacggt ggtgtgttgg
    31081 gtgaggcgtt aaattttggt ggtggctcac tgcttggctc tattttgcca agcggtgcgg
    31141 taactaccct atctcaaatc aaaacagcag tcagccgagg tgtggagctt tataacagcg
    31201 gcaaacgctt agttgacgaa gtgcaaaata ccgtggcgat tattcggcag ttgaaaaatg
    31261 acccggcaac tgcattggca tatttacccg ctgcattagc gaatttagac ggggcattgg
    31321 ggcattttgg taatttgacc ggctcatcgg cgaccttggg cggtttgcag gagaaactac
    31381 cggcagcagt ggtctttagc gaggaaatcg gcgagattta tcagagtttg caaacaatgc
    31441 gaagcagttt gggtaatgcc tcggcaaata gctgggataa ttggtttaca ccggcagaga
    31501 gtgcattgac agaggtcaca gacagctttg accatctagc aaaaccggtg gcaaaaatga
    31561 ccgcttggat tgtgctgcgt accgatgagg aggcagaaaa tgacacagtt gcttaaacat
    31621 atcgtcaaac agggtgagcg ttgggataac ttgagctatc aatactacgg caatgcgttt
    31681 gaatatggtc gaattattga tgccaatccg catatcggtt tttgcgaggt gttgccgata
    31741 ggggtaacaa tttatatccc tgtacttaac gttaagccaa cgaataatga aaatatgcca
    31801 ccgtggctaa gggggcgtga tgcgtaatgt accgaagccc gatttttcac tgttttatga
    31861 aaaaaccaat atcactgccg agattgagcc acatttggtg caactgacct atatcgacca
    31921 tttggaaggt caatcggacg aactcactgt ggaatttgaa gacattcaag gcaaatggat
    31981 acgccaatgg ttcccgactc agggggataa gctcaaagcg gcgattggct atcagggcga
    32041 atttctgacg gacatcggtg agtttgaaat tgatgaagtg gaatatagtt ataagccttc
    32101 cactattaac ttaaaagctc taagcaccgg tatcagcaaa gccaaccgta ctttaaaacc
    32161 gaaagcctat gagaatacga ctcttgctca ggtggtggca aaggtagcag ataacctgaa
    32221 attaaaggta gtcggtaaga ttaaggctat cccgattaaa cggatcaccc aatatcaaga
    32281 gcgggacgtg gagtttttgg cacgccttgc ccgagagtat catcacagct ttaaaatcgt
    32341 gggcaatcag ctggtcttta ccgataaaag cgaactgggg caaagtgagc cggtgttaat
    32401 gttggaagaa cgggatacta tttccctacg tttgcgagat cggattaaag acactgcaaa
    32461 ggcagtggaa atcagcggct atgatacaag cggtaaaaaa gtggtgaaaa aacgcaaaaa
    32521 ggcaaaagcc ttacgcccga atataaaaca agctaaatca gccagtgaag atacgctgaa
    32581 aatcatcaca cgaggcgaaa gtcaagaaca gattgatgca cggggtgagg ctgcgttggc
    32641 tacgcaaaat gaagatcagg cggctggtga tattgagctt atcggcaatc ctaaattggt
    32701 tgccggagca acgattttat tgcgtaactt tggcgtgttt agtggcaagt atctgattaa
    32761 atcatcacgc catacgatta gccgtagcgg tggttatact actcatattg aggtcagaat
    32821 gttggagttt attgccgatg atttaatcac attaggtatg gaggctggca atgcaaacgc
    32881 ataattttgg ggcgacttat caagaaggga ttgtatccgc tgttgatccg aaaacgcaca
    32941 aagtgcggtg taaaatccct gcgctggaag atttagaaac tgcttggctg tcgtttttga
    33001 ccccgaacgc cggtggcaat cagttttact gtttgcccga tgtgggcgag ctggtggcaa
    33061 tgctacttga tgcacaaggc gaaggtggct gtgtgtttgg ggcaatttat aacgagcaag
    33121 acccgacacc ggttgccgat agcgatatgt gggttaagaa attcagcaat ggcacggaga
    33181 tttcgcataa tcgcaaaacc ggcgatgtgg tagtgaagac taacgggcat ttaaccgcaa
    33241 cggctcaagg tggggctacg attaatgcaa acaccgtagt caatggcaca ttgcacgcta
    33301 ccggtaaaat cacctctgac gaagaagtct ctgctcctgt tgtgaaacaa ggcacggtag
    33361 agcttggcac acataaacac ggcggtagcc ctgaacctaa taagtaattc tttaaaccag
    33421 tttaaaatcc cttttaaaga tagccctgta tcatcagggc tatgaacaga caaacttcca
    33481 ttcaattaac ccactggcaa cttgcaccct caaccgatac agttgaggtg gtgcaaggta
    33541 ttgatgatat tcatctttgt attgtcaata tcctttcaac ccaaaaaggc acggatattt
    33601 tacgccctga atttggctct gaccactttc gctacattga ctaccctgaa gatgtggcta
    33661 tcccgaattt tgtacgggaa attacaaccg ccttgcaaaa atgggaaaaa cgcattgagg
    33721 ttgaagaagt attggtggac ggttcagctc cgcattttat ttttactgtg atgtggcact
    33781 taaccgatga tgtttatcgt gaaatttacc gaacaacggt ataaggatag gcaatgacag
    33841 aagcgattaa aatcattgat gacgatgtaa aaaccgtact tgccgaagcg attgccgatt
    33901 atgaaaaacg caccggtaaa atcttacaac ctgcacatat tgagcgttcc attattcaaa
    33961 cctacgctta ccgtgagcag ttattacgca aaggcattaa tcacgccttt ttacaaacct
    34021 ttccgcagtt tgcgacgggg attgcgttgg atttgtgcgg ggaaactttt ggttgttatc
    34081 ggcttagaga taaacctgcc cgtactttac tacgcttttc agtaagtggt gaacatacag
    34141 ctatagttat ccctaaagga acgcaggtgg caattaatga cgagctatac ttttcaacgc
    34201 tcaatgacga tgttattact ccattaatta cctatgtgga aattgatgca gagtgtaatc
    34261 aggttggtgt tatcggtaat ggttgggaat caggaagagt cagtaaacta aaaacagcac
    34321 tggcaactaa ttataaagta acagttgaaa acattgatat tacgagcggt gggttgatgc
    34381 aggaagaaga tgaaccttac cgtaagcgaa ttttagccgc accggaagcc tttagtaact
    34441 gtggctctat cgctgcatat gattaccacg tccgctctgt atcccaagat attgcagatg
    34501 tgaatatctc tacgcctagg ggtggcttgg taagaattgc ggtattgacc caaaaagggt
    34561 taccggatac ccgattactt gatgatatta aaaaacacgt tagtgccgaa cggctacgcc
    34621 cactttgcga tatggtcgag gttattaagc ccacagaacg ccgttatcaa attacggcac
    34681 gtttaatatt gttagatggc taccgtgagg atttggtaaa agcaaaagcc cgagaggcat
    34741 tacaactcta tttgtcagac aagactaaaa aactcggttt agacattgtg ccgtcggcca
    34801 tcatttcagc tttaagagtt gagggtgttt acgatgtgaa tttaattcag ccagtcaaaa
    34861 ctattatcaa cgaaacagaa tgggcgaact gcacagcaat cagcgtagaa gtcgaagagg
    34921 agcgtagaga tggctagctt gcactatgcg gacattattg tgcgagaccc taaatataaa
    34981 gcattagcgg atttaagcaa gcggttaaat ttgctcgata cttcgcaaat tatgactacg
    35041 ctggtggatt tattgggcga cgaatgtatc ccattgctgg cagaaaaatg gtcggtaacc
    35101 ggctatgacg gcttattggt tgcagatagc gaaaactcaa agcgagcctt aattaaaaga
    35161 gcaatcgaac tacatcaact aaaaggcacg ccttggtcaa ttcgtgaagt attacgcaga
    35221 ctaggtttcg gagaggttga gattgacgag ggactgaaaa atcggctgta tgaaagcact
    35281 tatgtgagcg gaatccccga aaacgaacgc tgggcgcact atgcgattag gttgagtaac
    35341 ccgattacca atgagcaagc tcgcaatatc cgtaaggtct tacgcaattt tgctccggct
    35401 cgttgctctc ttgcagtttt ggattataaa gcagatccaa ttcgatataa caataaagca
    35461 acctacaacg gcacatataa ccacggttca agttaaggat aaattatggc aggtgtacaa
    35521 gaaaccgcac agtgggaaaa ccacgtttac agaattgaag aaaatgaccc agtccacgga
    35581 ggagaaaacg gtgtaaccaa taagccaatt aagcacctag caaaccgcac gctctatctg
    35641 cgtagattgc tcaccgaggc aggacaacgc attaacccga aaaaaatcac agctaccagc
    35701 aaaaatagta atgatttaac tgggcatact cacgagattg accacgcaag cctaacgcaa
    35761 aaaggcattg tgcagttaaa tgcgagtatc caatcaacta gcacaactga gtctgcaacc
    35821 ccttcagcag ttaaagcagc ctatgataaa gcagcaacag cagaacaaaa agcgacattg
    35881 gcaaatgaga aagtcgaggc gattaaagta ggcggacgaa acctcctcat tcaatccaac
    35941 actccacaca acgggaatgc gtactcagtc agatacgcat tggctgaggc tccgaaagta
    36001 ggcgaagaga ttgtagtcac tctatggggt gaactcggct ctcaacgtac tggaattggc
    36061 gtttacaact ctcaaggtgc tacggagttg gcaaaactgg tcaaaattcg tgatggagtt
    36121 tatcaaggta aaggcatttg gcgtaagccg atgagtgagg gacgagagcg aacgcctaac
    36181 gacacgcacc tgaatgtgta tttttacccg aaaaccgcaa cagataataa ccgtattgag
    36241 cgaatccagc ttgagcgagg ctcaatcggt acagattgga cgccaaacga ggcagactta
    36301 aaggcagaga ttagtgcaat ccgcactaaa ctaatccctg tacggggtgg caatgttgct
    36361 acaaccttaa atgcggctaa gcggtcggat tggtttagag aaatgcaaat cccttatcaa
    36421 agtggttggg ggtattacac ccaagcattt cataactcag ggacgcaaca caactacagc
    36481 aatttaccgc tgacaggtcg ttattatttt atcgccgaac tctatcataa ctctggttat
    36541 tctcacttgc gtatcaccta tccaagttta aaacgcacct ttgagagtgc tatcaacctt
    36601 aacaatgagg atttgattct agattggcgt gaggtggtgt ttttaaaaga tggattgtat
    36661 caaggcactt ttaaaacaac aggcacacta gagtctgctt atcgtctaag agcggctcgt
    36721 gatgatgaac gttatcagcc atacctgcaa atggtcgata tgggctatga ccttgccaat
    36781 cccccggtag gaaaaaacaa aattattggc agtgttgatt attacgttaa aaacggcaca
    36841 agcgagtctt ccaaagcgac gatgatggca gcggcaatgc ctgacaagaa cgtattttgg
    36901 gaagtagcat tgtggaatgc agcacagcaa cggaatgtat tgtttaaggg gtatagcaag
    36961 actaacaatt tgagtattgg taaaaccacc gacaatgagc gagaccgagt acaagtcaat
    37021 ggcacaatcc aagccaccgc accggctgat aacgccaata acgaccaagt gccgactacg
    37081 gcttgggtgc gtaagatggc aaagatttta gctgatggca aggtgagtaa agccggtgac
    37141 acaatgactg ggcatttatt catcaagaat ggggaatact ctgcggtaca tacctataac
    37201 acaagtggtt ggttcgcaaa atgggaggcg gccccattat ctgattcgca ctttgctgcc
    37261 attgtatatg ccaacgacag agagacagtt atcaatcggg tgttaattcc caagaagaat
    37321 ggcacagtcg ctttaattaa tgatattgcc gataccgtta gagccgctgt gagtcgtagt
    37381 gataaatccc gtagtagaac ttttcctctg caaacgggga aaaaatatac aaccacaggg
    37441 agcgttacga tttatccaga tggccgaatt gtgcagattt tccacttaaa aaatattaaa
    37501 gcggcgtggt ttgattatga ggcaagtgcg gtaggagata ggcatagagt tgtcaatatc
    37561 cctctttgga ctgcgatgcc aaataaaatt tttgatgtcg atgttaaaac tgttcgcccc
    37621 tcaaataatg cctcaactta ctacatagaa gcggctgaat ggctgagtgc gtggcagatt
    37681 tacggtaatg acacgaacaa atcaagtgtc aatattaatc tatctcgatt cagaggtgga
    37741 aaggatgaag atatggactt gtatgtaaca gttgagggat attaaatggc atattttgtg
    37801 aatgtgattg atgagcaagg caattatgag ctaattgatg atgagtttat tgggctgtat
    37861 ccggatttgg attttaacac attacccaaa ctcacggata agcaatatca gacctacctt
    37921 gccaaagcaa acggcaaaga gcgatttgta aatggcgaat ttgtgtatga gcaaattaag
    37981 gtggatatgc aagcggtcat ttccgctgaa aaatctgcaa agttagcaga gattaaccaa
    38041 aaagcacaag cctttatcaa tgatttagcc aaatacaacg aaacgcctaa gtttgagcgt
    38101 gatacttggc ttgagcaagc gaaagaggca aaagcgtggg tggctgaccc aaccgtacaa
    38161 acgccaaccc ttgagttaat tgcgcaaatg cgaggcatac cgctcgacac gctacgccaa
    38221 aaagcctacg aaaaagcgat ggcgtatcaa acggtggctg ctattgtagc agggcaacgg
    38281 caaggatacg aagaccgcct agaacaagcg gaaaccttag agcaaataca ggcgattgag
    38341 ccgatgtatc aattaccgca aggagcaaac aatgagcaaa ctgactaaat taaaatcgtg
    38401 gagctaccac gtcttaattg caatagatca actctttaac gctctcaccg gtggagcagc
    38461 ggacgaaaca ttttctagcc gttgctaccg tggtgcagtg cttgcggaaa atccgaaaaa
    38521 acgttggcgt ttttggcact cttttttgaa ttgcttattt ttagacccaa agcactgcta
    38581 cgccgcttat aaagcggagg tacaccgcaa gcagtatccg aaggcattta gacttggggg
    38641 gatttaatga aaaagcaaaa cttaaaatta gtgcggggcg atgatgtgag ttttagattc
    38701 agcattaaag acagcaccgg acaacctctt gcgttaaagg atttgtggtt tgatttgcac
    38761 ttagtcagct atcaaggtga ggtgctgatt gctctctcta ctgatgatgg cagtattgag
    38821 acagcagagg ctggtcagat ggtgttacat tttagtcacc agcaaactgc tcgagctgaa
    38881 tggacaagtg ccaattatga cttgcaactc acgcacgact atacaagagt ctaggaggtg
    38941 gagatgacct ctatcattga actagacctg actgatgacc tcatcattga ggtgcagtta
    39001 gatagcacaa tcattaccgg cgatagcttt ttagccaacc tgccggattt actccaaatt
    39061 tacaaccaag caaagtcaga tagcagtaag gagcttaaca atgactaaac acaaaaacca
    39121 cgaaaaccac ccgattgcta taacattcta ccaacttggc aaagatgtcg gcgacattaa
    39181 ccgccggcta gcggttattg agcaacgtga gcaatcagca cagccaagcc aaccacaagc
    39241 tgaggcggtt acaatgccac agcaagatgc tgttgccatt aataacatca atgccgtttt
    39301 ggaggcggtc gaaaaataca aaacttttga aaaatacaac actcagtggc aagagcgttt
    39361 tgaaaaattc ctgtctcgta acccgaatga ttacgagtta agtgaagagc aacgccaaaa
    39421 actttaccac acgatgtgcc agcatttgtg gcaggtgaac tttactgaaa tcaatagtaa
    39481 acagtatgaa ttaccgatcc cgcaacaatt ccagggtaag tttggctata ttgtcaaaac
    39541 ctcgggcggt agcacagaaa ttagttttga gttgctatct gaaactcaca gcacttatct
    39601 ttatccggat agctatattc ccgagggcgg tggcgatgta atgctcaagc cggcttatat
    39661 ttatttagtg attccggact tagtggcaat gcgtgagccg gaggtggtgg gggtttcacc
    39721 gctgaaactg aacattctgc agaaacacct gctgacacct cagaaactac aggagcagaa
    39781 gcccttgaac acgaaggcaa actgattgat ggcgtatggc attttgagcg agagccggta
    39841 aatccaccga gagatcagaa ttattatgtg gcaggtaacc tgttctacaa cggcataccg
    39901 gattgataaa ttgatgtttc agtaaaccta attagggggg aatccccctt ttttattatt
    39961 ataaggaaag aaaatgaata aatctttagt agcgttagct gttgcagcga tggcatcgaa
    40021 agcacaaact aaacagcaag caagcaagca agcaagcaag caagcaagca agcaagcaag
    40081 caagcaagca agcaagcaag caagcaagca agcaagcaag caagcattgt gagatagaga
    40141 aagtatttaa acaagctcct ttaccctttt cagggcaaaa acggatgttt ttatcccatt
    40201 tcaaacaggt gttaaatgca aatattgaag cagacgggaa agattggact attatagatg
    40261 tttttggtgg gagtgggttg cttgcacata ccgcaaagcg tgagaaaccg ttagcaagag
    40321 tgatttataa tgattttgat aattacgccg aacggctaaa ccatatcaaa gagacaaatc
    40381 aacttcgcca agaaatctac caaattgttg atgaaattat accaaaaaat aagagaatta
    40441 gcaatgaaat taaagctaaa atcatcaata aaatcaatga ttttgagggg tttaaagacc
    40501 tcaaatgctt aagttcttgg ttattgttta gcggtgagca agttgcaact ttagatgagt
    40561 tatttaagca cgatttttgg cactgtgtgc ggcaaagtga ctatcctgaa gcaacgggtt
    40621 atcttgatga tattgaggtg gttagtgagt cctttcatca attattgccg agatttcacg
    40681 acaaagagaa agtattattg attcttgacc caccttatct ttgcacaaga caggaaagct
    40741 ataagcagga aaattacttt gatttaattg atttcttacg gttgatagac cttactaaac
    40801 caccttatat cttctttagc tcaaccaaaa gcgagtttat tcgctttatt cactatatgg
    40861 ttcaaaacag aaaagaaaac tggcaagcgt ttgagggagc ggaaagaata gtggttaatg
    40921 cctcggcaag ttataacggc aaatatgagg ataatatggt gtataagttc taaaaattta
    40981 aacgcccttt gatagtgatt taaagggcgt taaaaaacca ttaaaattaa gaaagtaaag
    41041 ctcctgtttt ctttcgtagt tttcgtggtt gcgtttcgca gttttgttgg ctcgctacaa
    41101 ttactgttca ttgtaatgtt taatcgacca ttggttttgc taggatcagt ttcaccaaaa
    41161 taaccgttaa aaccttggac ggtgccttct gctgctttga ttttttcagc aagtatttgt
    41221 tgctttgcag cactttcatc tgttaccttg agtacttcaa agtattcgtt ttcacgaatg
    41281 agaccagtag caaagtctaa atcgatgaaa actttacgcg cattaaaaat agctgtgatc
    41341 tctgcaacgc ttaaaggttt tttcaaacgg ataaaattag ttggcaagtg tggaaaacgt
    41401 tgatatgtga aaatatcttt gtccaaatga cgagaaacct gatctggact agtaattgct
    41461 tgaactttca cttggtcaat tgttggacta actatgccgg ttagtgtaat gtttgctgtt
    41521 tgggctgtat tatgattgac aaccatcgct ccttcatcgc tattctgaat acggcggaac
    41581 gtgagatcat tgccgttgac atctaagcgc ccaccacgat aaccaaaata gatattgtta
    41641 ggatccattt gatctgatga attgaggatt acggtaggtc tgccactcac aatacctagc
    41701 tctgaaaatg cagatttttt gttattttca tccgcttgtt gatttaatat aacagtgccg
    41761 tcaccaacgc taacggagcc tttgttttct cctttgccgt tgatagttag tgtcccttgt
    41821 ccgatttttg ataatctgtc accagcaggg ttactgattt tccagatcac atttttatct
    41881 ttatcaatat caacaccggc tcctaaccaa gtaatgcctg ctttcatacc ttccacagtg
    41941 gtattaccat gaaaatggat tgctcctgca ccttggttga tattctctgt gagacttagc
    42001 gtattgtttt cacccaaaat atggaatgtt ttaccgtgtt ggagttgagg attccaaggg
    42061 ttatcgcctg tattaccatt atgaccttga agtgttggta atgaagtatc tgctaaatta
    42121 actgtaagag gagcattccc tcctacgatt gagctggtat tgccagaagg tgtccaagtg
    42181 agtctagcac catttgcatt aatagcgcct gcattatttt ctttttctaa agcacgtaca
    42241 taatgtgggt ggtgatagtt ccagtaagat ttataaacat ctcccccaag cgtattattc
    42301 tctgagaaat agtcactata tacacctaac acaacccatt ttttggtacg aacatcataa
    42361 ccaaaaagag cggagccact atcgccaggt aaaccgagag ttgttaaggg accgtagccg
    42421 tggtctttaa atacatcgtt tatggtatta ttttcagttt ttgctactaa aatacggctg
    42481 ttaaaatcgc tgcctggaac gcttggatcg ccattagtaa caggcagaac gctacctcct
    42541 gtcaaaaaag gaccgttacc gacttcaata cgcccgttgt ttaagcgatc tctttcgttg
    42601 ttaaccactg cttgtctacc agaaccggca cgaataaaca tggggaaaat aaaagggtcg
    42661 ctaaatacac tataaaaatc ttgtacattt tcggtagtgc gttcaatctc attagctgga
    42721 gcgacttcag ttactaattt cgataaccta ggtgcgtggt agtcccagcg gggcgttggg
    42781 ttacctgaac catctaaaat cagtttttct ccttcgtcat tacgttcacg tttttctatg
    42841 cctttcccga gatctgttct ttctttattt ggatcaggca tatagttatt acggcgcacc
    42901 acactataat tgtaatgatg gtcgtcggct ttttcgctag atgaatcacc aaattgaatg
    42961 tcccgatagc cttggttgtg gtttacacta ataacgtatt gtggattaac taatgttgca
    43021 atccctgttt gacgagtaat ggaagagaag tcaatcattg gaatagacat agtctcacct
    43081 gttactgttt caaaaacagg tagatcatta gagtcttcat cacctccacc atagagaaat
    43141 tcataacaac cgggtaaact ttcattatca aagcattcag ataggcttac attcggctta
    43201 ggtgaaatat ttgattccac ttcaacctgc ttagttggtg taacctgtaa aaaagtaccg
    43261 atgtttttgc ccgttttatc aataactgga atattggttg ccccaacaaa aaatttgcct
    43321 ttattttctg ctaaatcacg aaaatattga taatcgacat caccacgtac aatagatgcc
    43381 agtaatgttg gtgaaagaga aatagcaatt gatgttgcta gaagagagag cttgaaagta
    43441 tgcttcatta ttatttgaaa cctgtataaa tatgtaagta gatcagattt gagcgctatt
    43501 aaaaagatta tttaaatcga ttaaatattc atttaaatac cacacaatta ttcagcgcga
    43561 ttatcttgtt cggcataaat ctgctcaata cccaaataag gtaataatta aaataatagg
    43621 ggatatttag tagtaacata gagaagagaa aagagtcttt attatgttat ttagagaata
    43681 attttgcgat tattactttt gcaaagtttt tcactttttc gaccgcttgt taaataaaaa
    43741 gggcgaaaaa tgtttcgccc ctaccgatta ctcttgtgga attgtacgga ttgctaactc
    43801 ttccaacgct ttcggattcg catagcttgg tgcttctgtc attaaacacg ctgcagcggt
    43861 ggttttcggg aaagcgatga catcacgaat gttatcagtt cctgtaatca acatcgttaa
    43921 gcggtctaaa ccgaaggcta agcctgcgtg tggcggagta ccgaatttta acgcatctaa
    43981 taagaaaccg aatttctctt tttgctcttc ttcgttaatg cctaaaatgc tgaacacggt
    44041 ttgttgcatc tgctggctga aaatacgtac cgagccaccg cccacttcgt agccgttaat
    44101 taccatatcg taagcgtttg ccaccgcatt ttccggatct tgcactaatt cttccggcga
    44161 taattctttc ggtgaagtga acgggtggtg cattgccgat aagttgcctt catcatcacg
    44221 ttcaaacatt gggaaatcaa tcacccataa cggtttccac gctgtggtat cggttaagcc
    44281 taaatcgttc cccactttta agcgtaacgc accgatggcg ttagtcgcaa cgtgccagtt
    44341 atccgcaccg aagaatacaa tgtcgtcttt ttgcaagttt aagcgtttaa ctaaggcttt
    44401 tagcacttct tcatttaaga attttgcaac cgggctttga atgccttcta aacctgtgtt
    44461 catatcattc acttttaacc aagccaagcc ttttgcccca taaatgccga caaattgggt
    44521 gtagtcatca atatttttac gggttagagc tgcaccgttt ggcacacgaa gtgccactac
    44581 acgccctttc ggatcattcg ccggaccgct aaatactttg aaatcaaccg attttaaaat
    44641 atctgccaca tcaaccattt ccagtgggtt acgcaaatcc ggtttgtctg aaccgaaacg
    44701 ggtcattgct tcattccacg tcatcattgg gaatttaccg agatcaacgc ctaaggtctc
    44761 tttccataag ccgtggatca tcgcttccat taatgcacgc acttcttcgg cagtcataaa
    44821 gctggtttcc acgtctattt gggtaaattc cggctggcgg tcggcacgca aatcttcatc
    44881 acggaaacat ttcacgattt ggtaataacg gtcgaagccc gacatcatta gcaactgttt
    44941 aaatagctga ggtgattgtg gcagagcata gaatttgcct ttatgcacac ggcttggcac
    45001 gaggtaatct ctcgcacctt caggagtagc tttggtcagc atcggggttt caatgtctaa
    45061 gaaaccgtta tcgtccataa aacggcggac aaaactggtg attttagcac gggttttgag
    45121 cttttctgcc atttcagggc ggcgtaaatc caaatagcgg tattttaaac gctgttcttc
    45181 ggtattgttt tgattgaagt ctaacggcaa tacttcagcg ttgttataca ccactaaatt
    45241 ttttactaac acttcaattt caccggttgc catctcttta ttgatttgcg attcatcacg
    45301 ggcaatcact tcaccttgaa tttgcacgca cgcctcagaa cgtaaggccg atgcttgctt
    45361 gaaaagtgct tcatctttct catcgaaaaa cacctgcaca atgccttcac gatcacgaat
    45421 ttgcataaaa ataaaacgac caaggttacg aacacgatga acccaaccgc ttaaggtaac
    45481 tgtttgtcca aggtgtgagc ggtttaatgc accgcagtag tgagaacgca tcataaaatt
    45541 atccttttga aaataaatac cccaagtttc ggggaaaact taaggattat agcggaaaat
    45601 tcagcgaaga tggataaata aaagcaaaca agcggtgatt tttattccat tttttgcaaa
    45661 aagtgggata aaaatcaccg cttgtgattt atccttccag cccaataaaa acggctaata
    45721 acaatggagc ggctaaatta acaataaaac cgaagctgat tgccagcggc acaatttgaa
    45781 taccgcccga tttttgaata atagggagca tacaatctaa tgaagtcgct ccgccaatgc
    45841 cgactgcggt ggacggaaat tgacgcataa acagtggtac taaaaacagg cataaaattt
    45901 cacgcgagat gtcgttaaaa aaggcaatac tgccgtaaat cgggccccaa gcatcgttta
    45961 ccaatacgct tgaaagggaa taccaaccga atgcagaggc aaaggttaag cctttagcaa
    46021 gggataaatc taaggtgaat gccgaaatca cgccaccaat cagggaactt gccaccatta
    46081 caatcgaggt ataaatcccc cgcttgttaa agaacacttc acgcagggga ataccgctgt
    46141 ttcgcagttg aatccccacc ccgaaaatca tcacttctaa cacataggtg ctggcgtgca
    46201 gagggaaatc caccacgcct ttggtgaaat agccgatgat cccgcctaat aaggtaatgc
    46261 cgattaattt aaaggaatca atcaacaaat gcaggcggga gggaagttga tcttgattga
    46321 ctttgtctct tttcatcggt tgtaatttgt cgtaaatcac caagccgata atgtttgaaa
    46381 ggagtaaaat caccactaaa ctaaacgccg taccgccaat ctgtggcagt tcggtgagaa
    46441 tgttatcaat ttgtccgaga gaaatcccca tcactaataa aatcaagtaa aggcagaggc
    46501 tgaccactct gtttactatt gccatatggc ggtggttttt gagggttatt aagtaaccga
    46561 gaaaaagtgg aatcagcaca atggctaaac cgtagagcat tttttatcct ttttatcgct
    46621 atttttaaac ttgaaattct aacaataagg gcataaaata aaactgtttt tatatacagt
    46681 tatttgtgtt gcaagcggtc gtttttgggg aaaatattgc aaatgtcgat tcaagctaaa
    46741 cataagcgaa aaattatcca tattgatatg gattgttttt atgcagcggt cgaaatgcgg
    46801 gaaaacccgg cgttggtcgg taagccggtg gcggtgggcg gcaatgctaa tcaacgaggt
    46861 gtgctgacaa cctgtaatta cgaggctcga aaattcggct tacacagtgc aatgccaacg
    46921 gcaactgcgt tgaagaaatg cccgaattta attttattgc cggtgaatat ggcgttgtat
    46981 aaatcggttt ccgctcaaat tcacgaaatt tttcatcgct atacggatgt gattgaagcc
    47041 ttgtcgcttg atgaagccta tttagatgta accgaatgcg aacgctattt tggctccgcc
    47101 acttggattg ctcaagcaat ccgcaatgat attttcaatg aattgcattt aactgcctcg
    47161 gcaggagttg ctccgcttaa atttttagca aaaattacct ccgatcaaaa caaacctaac
    47221 ggacaatgtg tgattacgcc tgatgatgtg caaaattttg tgaaaaatct accgcttgca
    47281 aaaattccgg gtgtggggaa agtaaccaat gaaaaattaa aacagcttgg ctttgaaacc
    47341 tgtggtgata ttcagcaagc agaaaaagcg gttatttacc gagcgtttgg caaattcggg
    47401 caacggcttt gggattttag ccacggtatt gatgaacgag cggtagagcc ttatcgtgaa
    47461 agaaaatcgc tggcggtgga aaatacgctg ttgcacgata tttcaaccct tgagcaagcg
    47521 gaaaagattg tggcagattt attcgacaaa ctggtttttc ggttgcaacg aaactggggc
    47581 gatacgccat taactgagtt taaaaagtta ggcattaaac tcaagtttga tgattttagc
    47641 caaaccacgc ttgaacgaac caccgatggc tttgcgtatg agcgatttgg cgagttgcta
    47701 cagcaaattt ttacccgaag aaaggaacga aaggtgcgat taatcggtct aagtgttcac
    47761 tttcccgaga aaaaagtgaa taaacagtta aatttgtggg aataattgta aataatgact
    47821 tgaaattaag gctaaagtca ttatcttatt cgtgcaatca ttgcatctat atttttggag
    47881 attgaaaaca ttatggcaaa acgtgtcatt ctatttttat taactaactt agcgattacc
    47941 tttgttttag ggatcgtatt aaaccttatt tttaaagtga ccggcattca agggcaaagc
    48001 actgccggta ttttggtgct ttcgttagtc ttcggtttca gtgggtcttt aatttcgcta
    48061 tttatgtcga aatcaatggc attgcgttcg gttggggcag aggtgattac acagcctcgt
    48121 aacgaagcgg agcaatggtt attcaacact gtacaacgtc aggctcagca agcgaatatc
    48181 ccaatgccgg acattgcgat ttaccattcg gctgatgtaa atgcattcgc tacaggagca
    48241 acgaaaagta attctttagt tgcagtcagc accggcttgc tgaattcaat gactaaagac
    48301 gaagctgaag cggtagtagc acacgaagtg gcgcacattg cgaatggcga tatggttaca
    48361 atgactttat tacaaggcgt gttaaatact ttcgtcattt tcttagctcg tattatttcg
    48421 acaatggcgg caagttcacg tgacgaaaat ggcaattcaa gcaccaatac gctggtgttc
    48481 tgggtagtag atattgtgtt acagatggtg tttggtgtgt tagcaacaat gattgcaatg
    48541 tggttctctc gccaacgtga atttcgtgca gatgcaggct ctgctcaatt agtcggtaaa
    48601 gagaaaatga ttgcggcatt acaacgcttg caacgtgttc acgaaccgca agaaatggaa
    48661 ggctctcttg ctgcctttat gattaacggt agccgtggta aagagttgtt tatgagccac
    48721 cctccgttag aaaaacgtat tgaagcgtta agaaacttat aaagcacaag cggttgtttt
    48781 tgtaaaattt tttgcaaaac agccgctttt gttttctata aaatatgcgg taacaccgaa
    48841 tagagagttg ccagcaaaaa cagataacgt aacaatttcc caatcacaat aaagagaatg
    48901 ctttgccaaa ggctaaaccg tagccagccg gcaatgccac agaaaatatc gcctacaatc
    48961 ggtaaccaac taaaaagcaa gactaacacg ccatattttt cacttttggc taatgcccat
    49021 tgtgaataac gattggtagt attctgtggg cgagggagta atagccctat cgcataagtg
    49081 gttatgctac ctaaactatt acctaaggtt gcaaccaaga gtaaattgaa cagtgaaaat
    49141 gaggataaag agccataagt gatgagatgt tggcttgcta aggtagtgaa gataatttca
    49201 gaatttccgg gtaagagggt agcgcttaga aatccactca gaaacatagt aaagagttga
    49261 ttttgttcgg aaaagaataa actgaaaaag gaagtgagat attctgtcat aaaattgtag
    49321 ttggtttgtt attaaaatgt agttataaat tttcttgaat gttgtgtttt ttatggattt
    49381 tgttctatat caaaaaaaga catcgaaata cctctgattg tgtaatattt ttatttgaag
    49441 attatctcaa gaggtctata atttctacat aatatatcgg tttttcaatt ctcattgtta
    49501 aggttgtctg atgttttctc ctgctgaaat ggcaaaaatc gcagaagatg gtgcggttta
    49561 taaagcaaca aaaaatcaat tatattcctt tctctctgcc attactgcgg gcggttttat
    49621 tgcaatagca tttgtatttt ataccacaac ccaagtgggt gcgagccaaa tgccttgggg
    49681 tatggctaaa ctgattggtg gtttggtatt ttcactcggc gtgattatgt gtgtggtgtt
    49741 tggggcagaa ctttttacct catcaacact tacgattgta gccagagcca gccaaagaat
    49801 taactggtta cagatgttta aaaactgggt agtggtgtat ttcggtaact ttgttggggc
    49861 gatttccatt gtattgttga tttggtttag tggtcagatt atggcagcca aagggcaatg
    49921 gggtttggtt attttaaata ccgcacagca caaaattcac caccaatggt ttgaggcttt
    49981 ctgtttaggc attttctgta acattatggt gtgcattgcg gtgtggatgt cttatgcagg
    50041 taaaagccta accgataaag cctttattat gatgttaccg attgcaatgt ttgtggcttc
    50101 cggctttgaa cactgtgtgg caaatatgtt tatgattccg atggggattg cgatttcgca
    50161 ctttgccagc cctgaatttt ggacagcaat taatgttgac ccaacgcaat ttgccgattt
    50221 agatgtatct cattttgtgt ttaagaactt aattccggca acgctgggta atattgtcgg
    50281 tggcgtgttt ttcattggtt taatgcagtg gttcttatac attagaaagc attaagattt
    50341 cagattcaag cggtcaaatt ttgcaaattt tttgcaaaaa atcaccgctt tcccgtttta
    50401 acttactaac acagaggact taaaatgact caactaacag acgctcaaca aaaagcgtgg
    50461 gaaggttttg ccgcaggcga gtggcaaact gaagtaaatg tacgtgattt tatccaaaaa
    50521 aactacacac cttatgaggg tgatgagtct ttcctagctg aagtaacacc tgcaaccagc
    50581 aaattgtggg cagatgtgat ggaaatgatc aaagtagaaa acaaaacaca cgaaccgtat
    50641 gatattgact gcgacacgcc atcaaccatt acctcacacg caccgggtta tattgataaa
    50701 tcgttagaaa aaatcgttgg tctgcaaaca gatgctccat taaaacgtgc gattatgccg
    50761 tttggtggta tcaatatggt gaaaggttcg tgcaaagttt atcgccgtga gcttaaacct
    50821 gaagtagaac aaatctttac cgaataccgt aaaacccata accaaggggt attcgatgtt
    50881 tacactccgg atattttacg ttgccgtaaa tcaggtgtaa ttaccggttt gccggatgct
    50941 tatggtcgtg gtcgtattat cggtgactac cgccgtatgg cactttatgg tgcagacttc
    51001 ttaatgaaag acaaacaacg ccagtttgcc tcattacaag ctcgtttaga atccggcgaa
    51061 gatattcaag ccacgattca actgcgtgaa gaaattgccg agcaacaccg tgctttaggc
    51121 aaaatgaaag agatggctgc aagctacggt tttgatattt caggtcctgc aaccaatgca
    51181 caagaagcgg tgcaatggac atatttcgct taccttgcag cggttaaatc gcaaaacggt
    51241 gcggcgatgt ctttcggtcg tgtgtcttca ttcttagata tttatatcga acgtgactta
    51301 aaagccggca aaattaccga gcaagaagcc caagagttaa tcgaccattt agtaatgaaa
    51361 ttgcgtatgg tgcgtttctt gcgtactccg gaatacgatc aattattctc aggcgaccca
    51421 atgtgggcaa ccgaaacctt agccggtatg ggtttagacg gtcgtacctt agtaaccaaa
    51481 aacagcttcc gtattttaca caccctttac acaatggggc cgtcaccgga gccaaactta
    51541 actattcttt ggtctgaaca attaccggaa gcatttaaac gctactgtgc gaaagtgtct
    51601 attgatactt catcagttca atatgaaaac gatgacttaa tgcgtccgga tttccaaaac
    51661 gatgactatg cgattgcgtg ctgcgtatcg ccaatgatcg tgggtaaaat gatgcagttc
    51721 ttcggcgctc gtgcaaactt agcgaaaacc ttgttatacg caatcaatgg cggtgttgat
    51781 gagaaatcag gcgaccaagt agggcctaaa actgaagcaa ttacaagtga atacttagat
    51841 tacgatgatg taatgacccg cttagacagc tttatggatt ggcttgcaac ccaatatgtg
    51901 acagcactga acattatcca ctttatgcac gataaatatg catacgaagc ggcattaatg
    51961 gctcttcacg accgtgatgt gttccgcaca atggcgtgtg gtatcgcagg gctttcagta
    52021 gcggcagact cactttcagc cattaaatat gcgaaagtaa aaccaatccg taccgatatt
    52081 gaaatcaaaa acaaagctgg cgaagtggtt ggtgtagcga aaaacgtagc aaccgacttt
    52141 gaaattgagg gtgaatatcc gcaattcggt aacaacgata accgtgtgga cgacattgca
    52201 gttgatttag ttgaacgttt tatgaagaaa attcaaaaac tcaaaacata ccgcaatgca
    52261 acgccgactc agtctgtgct tactatcact tctaacgtgg tatatggtaa gaaaaccggt
    52321 aatacaccgg acggtcgtcg ctcaggtgct ccattcggac cgggtgcgaa cccaatgcac
    52381 ggtcgtgacc agaaaggtgc ggtagcttca ttaacttctg tggcgaaatt accgtttgcc
    52441 tatgcgaaag atggtatttc ttatactttc tctatcgtac caaatgcgtt aggtaaagat
    52501 tacgaagccc aaaaacgtaa ccttgcaggc ttaatggacg gctacttcca ccacgaagca
    52561 actgtagaag gcggtcagca cttaaatgtg aacgtgttaa accgtgatac attattagat
    52621 gcggtagaac accctgaaaa atatccgcaa ttaacgattc gtgtttcagg ctatgcggta
    52681 cgtttcaact ctttaaccaa agagcaacaa atggacgtaa ttacccgcac cttcactgaa
    52741 agtatgtaat tacaatttgc aaaactttta gccacaaaac gcattgcgtt ttgaacgttg
    52801 gctttgccga cggctccgta ggagcgaaca aaattggaaa caattttgtg agtaataatc
    52861 aaccgcttgt agcatttatg ccgcaagcgg tttttttgtg atctcgctcg aaaaattggc
    52921 tattcattta cacagctttc gtaatattgt ttataattac agtgttctat atgggagaaa
    52981 gagatgtcta agcaagattt aattcgttcc tctgtggcgg aatacctcac ttttattaca
    53041 gctaccggcg aaagccaagt tgatgccgtt tacgccgatg aaaatgtatg gcttagccaa
    53101 aaaatgatgg cggtgttgta tgatgttgat gtacgaacca ttagctatca tctcaaaaaa
    53161 atctttagtg acaatgagtt agctgaaaat tcagttatcc aatttttttg gataactgcc
    53221 caagacggca aaaactacca caccaagcat tataatctgt cggcaattat tgcggtgggt
    53281 tataaagtca attccgagcg agcggtgcag tttcgcaaat gggcaaccga aatcgtgcat
    53341 agctttgcga ttaaaggctt tgcgatggac gatgaacggc tgaaaaatga cggcacgatt
    53401 ttgggcaaaa aatactttga agaacagctt gctcgtattc gggaaattcg gttatccgaa
    53461 cgcaagttct accaaaaaat caccgacatt tacgctaccg ccgtggacta cgaccgcacc
    53521 gcaaccgcca ccaaacgctt ttttgccacc gtgcagaaca agctccattg ggcaattcac
    53581 gggcataccg ccgccgagct gattatggca cgagccaacg ctgaaaagcc gaatatgggg
    53641 ctaacgaatt ggaaagacgc tccgcaaggc aaaatttacc cctttgatgt ggtggttgcc
    53701 aaaaattatt tatcggataa tgaattagca caactacaac ggctggtctc tgcctatttg
    53761 gatatggcgg aagatatggc agaacgccaa attccgatga caatggcaga ctgggaaacc
    53821 cgccttaacc gctttcttgc cgccaccgac cgagaaatct tacaagatgc cggcaaagtt
    53881 accgctgaaa ttgctaaatc tttcgccctc agcgaatttg aaaaataccg agtcaagcag
    53941 gatctcacct acgaaagcga tttcgactta ctggtaaaag aggtcgccgc caaataccac
    54001 gccggctagg tttgcaaagt tttcagaaaa accgactgct tgtaagcggt caaaatagct
    54061 ttaaaaaatg caaaaattta aacatatttg gttgatatgt tgatggagtc aatagattaa
    54121 taaaagttta ttcctgaaat aggcaaaaaa tagtatgact ttctcctttc ttaaaatata
    54181 ccggtgtgat ttatttctgc gaacaacata aaaatttgct acaatttctc aaaatttttt
    54241 gagggttgag gtgacaaatg tctgttgttg gacgtatcca ttcttttgaa tcctgcggta
    54301 cggtagatgg accggggatt cgttttattc tgttcttgca aggctgtttg atgcggtgta
    54361 aatattgcca taatcgtgat acttgggatc ttgatggtgg taaggaaatt acggttgaag
    54421 agctgatgaa agaagtcacg acttataagc attttatgaa agcaaccggc ggtggtgtaa
    54481 cagcttcggg tggggaagcg gtgttgcaaa tggagtttgt gcgggattgg tttcgtgcct
    54541 gtaaggctga aggcattaat acctgcttag ataccaatgg ttttgttcgc cattatagcc
    54601 cggtggtgga tgaaatgctt gaagtgaccg atttggtctt gctggattta aaacagctca
    54661 atgatgaagt tcaccaagat ttagtcgggg tgtcgaataa gcgtaccctt gatttcgccc
    54721 gttatctgca caaattaaat aaaccgactt gggttcgcta tgtggtcgtg ccgggttata
    54781 ctgatgacga tgattctgcc catcgtttag ggcagtttat tcaaggaatg gataacattg
    54841 agaaagtgga acttctgcct tatcatcgtt taggggctca caaatgggaa accttaggct
    54901 ataaatatga gctggaaggg gtaatgtcac cgcctaaaga ggatttagag cgtatccaaa
    54961 aaatcattga aagttacggg catacagtca aattctaaag acaagcggtc atattttgtg
    55021 atttttttgc aaaaaattat tgaaatatga ccgcttgttt tcttttctct ctcactttgt
    55081 gtattatctt gatacgaatt ttcattttga ccataaaagg aaaaaataat gtctaaccct
    55141 cttttaaatt acacaggctt gcctgcgttc tcacaaatca aacctgaaca tatcaaaccg
    55201 gcggtagaag cggtgatcaa agaatgccgt gaaactgttg aaaaagtcgc acaaattgaa
    55261 aacccgacgt gggaaaattt ctatatgcca caagcaatgg cagaggataa attctcccgt
    55321 gcgtggtcac cggtagggca tttgaatgcg gtgaaaaatt ccccagagtt gcgtgaggct
    55381 taccaagcgt gcttgccgtt attatcggaa tacagcactt gggcggggca acatcaaggg
    55441 ttatatcaag gctatgtgaa gttaaaaaac agccctgagt ttgaaactta ttcggttgca
    55501 cagaaaaagg cgattgaaaa cagcttacaa gattttgaat tatcaggcat ttcactgccg
    55561 gcagagaaac aaaaacgcta cggggaaatt tctgcacgct tgtcggaact caactcgcaa
    55621 tttagcaaca acgtacttga tgcaacaatg ggttgggata ttgtaattac cgatgaagca
    55681 cagcttaaag gcttgccgga atcggcatta gaagcggcaa aattatccgc agaaagcaaa
    55741 ggtaagaaag gctatcgctt cactcttgaa atcccaagct atttgccggt gatgacgtat
    55801 tgtgaaaaca aagcactgcg tgaggaaatg taccgtgctt atgtgactcg tgcttctgat
    55861 caaggcccga atgcaggcaa atgggataac agtgcggtga ttgatgagat cttaaaactg
    55921 cgtcacgaaa aagcccaact tctcggcttt aagacttatt ctgattattc attagccacc
    55981 aaaatggcag aaaatccaca acaggtatta gatttcttga acgatttagc cgctcgctcg
    56041 aaagctcaag gcaaaaatga attatgcgat ttgaaaaaat ttgctaaagc ccatttcggc
    56101 attgaacatt tggatttatg ggatattccg ttctacagcg aaaaacaaaa acaggcattg
    56161 tattcggtca atgatgaaga acttcgcccg tatttcccgg aaaatcgcgt attatcaggc
    56221 ttatttgaag taattaaacg cattttcggt atgcgtgtgg aagaacaaaa agatgtggat
    56281 gtatggcaca aagatgtacg ttttttcaat atttttgata aaaatgaccg cttgcgtggc
    56341 tcgttctact tagatttata cgctcgtgaa aacaaacgtg gtggggcgtg gatggatgat
    56401 tgcatcaacc aaaaacgctt ggctgacggc acattacaaa aaccggtggc atatttaacc
    56461 tgtaacttca ataaaccgat tggcgataaa ccggcattgt ttacccacga tgaagtgaca
    56521 actttgttcc acgagtttgg acacggcatt caccatatgc taactgaaat tgacgtgggc
    56581 gatgtcgccg gcattaacgg tgtgccgtgg gacgcagtgg agctgccaag ccaattctta
    56641 gagaactggt gctgggaaga agaggcatta gcctttattt cagggcatta tgaaaccggc
    56701 gagccgttac caaaagcgaa attagagcaa ttattggcgg cgaaaaactt ccaagcggca
    56761 atgtttgtgc ttcgccaatt agaatttggc ttgtttgatt tccgcctaca caccgaatat
    56821 gacccgagca aacaaggaca agtgttagcc ttacttaaag aggtgaaaga ccaagtcgct
    56881 gttgccaaag gcgtagagtg ggcgagaacg ccacacagtt tctcgcatat ttttgcaggc
    56941 ggctatgcgg cgggctacta cagttattta tgggcggaag tgctttcagc agatgcctat
    57001 tcacgctttg aggaagaggg gattttcaac cctgaaacgg ggcagtcttt cttagataat
    57061 atcttaaccc gaggcggttc agaagagcca atggtgctgt tcgaacgctt ccgtgggcgt
    57121 aagccaaccc ttgatgcctt gctcagacac aaaggcattg taggtaatgc cgagcctgtt
    57181 gattgcactc aataaagtaa acacgtttta caagcggtta gatttgcaaa tttttttgta
    57241 aatttgaccg cttgtctttt ctttcttttg caactcgtta taattttcag catttagcct
    57301 gacagagagt gaaataatgt ctttagattt agccgatatt cgaacccaaa taaccaactt
    57361 agaccgcaat ttattaaagc tactggcaga acgccaccgc ttagcctttg atgtggtgcg
    57421 gagcaaagaa atcacacaaa aacccttgag agatttagaa cgggaaaaga ttttgcttca
    57481 agaactggta aattttgccg aagcggagaa ttaccagctt gatccgcaat atgtgaccca
    57541 aattttccaa cgtattattg aagattcagt gctgactcag caaaattatc tgcaaaataa
    57601 attgaatgca caaaaagaag agcaggtttc gattgcgttt ttgggaatgc gaggctctta
    57661 ttctaatatg gcatctcgac agtttgccaa aaaatatcaa ggcacattgg cggaattaag
    57721 ctgccattct tttaatgaag tatttgagaa agtcagcctt ggtgaagccg attttggcgt
    57781 actgccgctt gaaaacacga cttcaggttc gatcaatgaa gtgtatgatt tattgcagca
    57841 taccgattta accttagtgg gtgaattggc gtacccaatc aaacattgtg tattggcaaa
    57901 cggcacggtt gatttggcgg aggtggatac catttacacc caccctcagc ctgcccagca
    57961 atgtagccaa tttttagccg ggctagataa agtacatatc aaatattgcg aaagcagctc
    58021 gcacgcaatg caaatggtgg ctcgcttgaa taagccgaat attgttgcct tgggcaatga
    58081 agatggcgga aaattgtatg gcttgacgaa tatcaaaacc gatattgcta atcaagctca
    58141 caatatcacc cgttttattg ttgttgccaa agaggctgtg caggtttcgc cacaggtaca
    58201 aaccaaaacg ttactgttaa tgaccaccac gcaacaggcg ggtgcattgg tcgatgcgtt
    58261 aatggtattc aaaaatcatc atattcgtat gatgaaactg gaatcccgcc cgatttacgg
    58321 caaaccttgg gaagagatgt tttatgtgga gctggaggca aatattcatt ccgagaacac
    58381 ccaaaaagca ttggcggaat tggagcaagt taccagctac ctcaaagtgt tgggttgcta
    58441 cccaagcgaa attattgagc cggtaaaact gtaatattgt ctaatatgac cgaaattaaa
    58501 gctcagaata ttttctgagc ttttacttta ggttaagccg attcttgttt tagaattgca
    58561 taaagttggt cttttaaatg caatttttct ttttttagtg cctcaacttc ttcatgtgtt
    58621 gccagttcaa tatttgcctc catattctta atacgctggt ctagctcatt atgtttatca
    58681 aataagcgag caaaatgtga attgttttgc tttaattggg ttatgagatc acgaaattct
    58741 ggaaacatat ttaactccta atagtggttg caaattttac ttgattaaat actagcattt
    58801 ttttgttgat ttactgttaa atctttctat ttttttgaac ttgatcacaa aatttatatc
    58861 gaaaattata atgtgtgtga gccatattta aggctataat aatgagtagg aattttagat
    58921 ttaaggacac aaaaataatg agctataaag tggtcttttc gctgttgttt tctctatttt
    58981 tatcaacagc aaatgcaaat tggacaaaaa ttaatgatgt gaattatacg tgggggccat
    59041 ttaatattta ccatattacg ctttttactg agagcggtac ttatgtagaa ggtacacgtc
    59101 ctttaatgct tactctacaa tataaaaaac cgtgggaggg gcgtgatttt gcggtttctt
    59161 tagcaagaac ttggaataat ttaggcatta agctacctga aaaagagtta gatgaggtta
    59221 ttacccacct gagaaaaact ttcccagata ttaagcctga agatacattg cactatattg
    59281 cattagaaga tagaggttat tttattttaa atgataaagt agtctctgat gtgtttaata
    59341 aagctttcaa tgatgctatt gtagcaattt ggcttgatcc gaaaatggat attagccatc
    59401 aattacttga tccatcttat aaaccgagag cagaggcaga aagtattaag ccacaagtag
    59461 aggaaagagt agcggaaaac ttacaagaac ctatcgtaaa tactgaagtt tctaaagagc
    59521 tgaaagcgga ttctcaagca gtagagcaag caccaaacaa aatagatata cctgtagcag
    59581 ataaaactca agctagtgtg gtaaatgaag cgactcaacc ggtagagaat aaagataaga
    59641 aagagccaga aaagctagaa gaaaaaccaa aggagatgaa gattgaaaat ccggaagtgt
    59701 ttatttcacc gatttatgat gatatcttaa tgctcaccaa atattgtgtt taagcaaaga
    59761 acaagcggta atattctcga aaaatattac cgcttgttca ttttaataat ccatttaaga
    59821 taaaattttc gctaactcag cacttacttc ttccactggt tttgttccat ctaaacgctc
    59881 ataacgagtg ttgccggctt ctgcttctgc ggtgtagtag gcgattaacg gtttggtttg
    59941 tttgtggtaa atcgctaaac gctctaatac cgtttcaggt ttgtcatctt ggcggataat
    60001 taaatcctca ccagtcacat catctttgcc ttccactttc ggtggattgt acaccacgtg
    60061 gtaagtacgc cctgaaggtt ggtgaacacg acgaccactc atacgctcaa caatgacttc
    60121 atcagctaca tcaaactcta acactaaatc aatttttaca cctgactctt ttaacgcatc
    60181 agcttgcgga atggtgcgtg ggaaaccgtc taataagaag ccattagcac aatccggttg
    60241 agcaatacga tcttttacta aggcgaccgt taattcatcc ggcactaact tgccttcgtc
    60301 catcaatgct tttgcttgtt taccaagctc agtgctttct ttaatcgctg cacggaacat
    60361 atcgccggtt gaaatttgcg gaataccgaa tttgttcata ataaactgag cttgcgtacc
    60421 tttgcccgca cccggcgcac ctaataaaat cactttcatt tgaaaacctc aattaaacta
    60481 aaaataataa aatcacttag gcagtaacct taccgattac tgcctaaaat tgcaaatttt
    60541 tttaattttt tgaccgcttg ttatacggaa ttagaattca tagcgtaaat ttaagcgtgt
    60601 atagaatggc tcaccgtagc tatattggct aaagctgagg gcgttacgat attttttatc
    60661 gaataagtta tcaatgttaa gctgtgctga gaagttgtca ttaaattgat aacgagccat
    60721 caaattcact aaagtgtaag ctttttgacc atattcgcca taagtagcgt gtttggtata
    60781 aattctgctt tgccagttta cacctgctcc taccgttaaa ccttgcacta aacggttcat
    60841 atcgtaagtt gcaaagagtt taaattgttt gcgtggttga gttgtgttaa ttgggttacc
    60901 tgaagcatca tcgcctttcc actgagtata tcctgcactg agttttaatt tttcagttac
    60961 atttccagat gcttctactt caaagcctcg aactttcgca cctttagagg ctgtgtagta
    61021 ggctaataat gtattagggt taataccggc agattgagct agattatctt gtcttgtttc
    61081 aaatacgcta atttgagttt gcaagttgtc attgaaataa ctccctttcc aacctgcttc
    61141 gtaggtttcg cccgtaacag gagagagtag tttgttattg atgtccactt gactttgtgg
    61201 tttgaaaata tcggtataac ttacataaac ggtattttgt ggattaatat caaaggttaa
    61261 tcctgcataa ggtgtccaaa catcgttagc gtggaagttt tgtgttgaac catagaaagt
    61321 gccttctgtg cggtacgatg aaatacgaga acctagaagc atagaaagtc tatctgtaag
    61381 atgtaaacgg gtagaaacat acaccccgaa ctcttttaag ttttggtagt atttttcaga
    61441 catacttccc cagtttggtc tagcataaat gccattccaa ttattaaagt cgaaagctcc
    61501 gtcaaaggtg ctattatcgg cagcgtagga attacgatgt gctcgattgt attgaatacc
    61561 gattgccgca tcgtgttcta atccccaagc agagaatgaa ccggtaagat caagctgcac
    61621 attagattgt gtattatcat ttttaaattt acccggccaa ggcgaccaac ctaatccatc
    61681 ttggcgatta agtgatgagc cgctgaaata gaaaagttct gaattatgtt tgcctacacg
    61741 gtaattgcct tttaatgcaa gttcccattt ttcgcctagt ttttgtgtaa tatcggcaaa
    61801 atagtttacg gattcattat cccagtaagc ccattccgga ctggcatttt tgcttctgct
    61861 ccagttagtt tcaccaccgt cgctatagaa tgcgggtaaa ccaccccaaa gtgctgcatc
    61921 acgtttttct ttttgatagc ttacacctaa cccaagtttg gtagtgtcag ttaagtctgc
    61981 ttcaatattg ccataaagcg tggtgtaacg ttctttttca tttttaatga acgtattacc
    62041 gtttttatgc tctgcaataa agcggcctct tactgatcct gaattattca atgcttggct
    62101 atggtcaatc attgtattga aatcagcata acgcccgatt ccagcttcta aaatagtata
    62161 acgggaaatg ctattggctc gtttacgaac aagattaaca cttgcagaag gatcgcctgc
    62221 cccagtagtt aagcctgtag caccacgcac aacttctact cggtcgtata acacgctgtt
    62281 gcctgtattt tcgcccgttg tccattggtt atttacttac tttgagatcc ataccatcta
    62341 ccttgacttt atcaatccca aaaccacgag cactgaaaga atagcgtcct ctgtcaagta
    62401 aagaaacaga aacaccgtga gttgcttcaa gtgctttggc aatcgtatta agattttgat
    62461 ctctgatttg ttgatgcgtt acagccgtta cgctttgtgg cgtgtctttc gcactcaatg
    62521 ccattccatt agagcctttg gcttttttct gtgcataacg tccagtttct tccgatgagg
    62581 catttggcgt ttcggcactt acttctacgg tatcaagatg agtaactgtt tcagcgtaag
    62641 ctgttgagat aaaccctata ccagaactgt ataaggcaag ttttatcgct ttagatagcg
    62701 tgttcataat ttctcctaag taatgaataa aaaattattt atgttgtttg agtaacagta
    62761 agaaatagat acccccgaga atagaggcaa taatgcctgc cccaatctca taagggaaaa
    62821 ttagataacg cccgagccaa tcggcaatga gcattagcat tgcccctaat aatgccgata
    62881 tagggatctg tttctccggt gtaattgcac ctaaattttt agctaaatga ggggtcatta
    62941 gcccaatgaa gctcattggc cctaccgcaa gtgtagaggc ggcactcatt agggcaatta
    63001 aaagtaatac aatcatttgt acttttttaa cctctaaccc taaatggtaa gaaacggtct
    63061 cgccaagtga gatcaaacgt aaaggtttga taaacagtaa agtggcactg ataagtagga
    63121 ttgcaacaga taataaaatc caaactgtat tcggattagc gtaataggtt gagccagaca
    63181 gccaactaat aactgccgtt aaacgagggc tgccgcttag ttgaataata gccattaagc
    63241 cactgactaa agctccaata gcaacacctg taatcattaa cgctgatggt tgtaatttac
    63301 gagagagcca cataataaga gcgagaacag tcaaactccc taatacacca aataacaaga
    63361 agctaatgct agataatgtt gggaaaagta agaatgacag tactgcagca atagttgccc
    63421 ctgaactcac acctaatact tcagggcttg ccattgggtt tctcgttaag atttgtaata
    63481 ggctaccact taccgcaagc attgcccctg ttgctatcgc acttagacta cgaggcaagc
    63541 ggtaggaact catcaggttt tcatcaatgc taatagccca gccattcatt gttggagcaa
    63601 tgatttgtaa tagacaaaat aggataactc cagcgcataa caacaatatt ggcagtttcc
    63661 acgaattgag gcgtttaacg gggaagtatg ctgtattgct tgcttcaaat tgtgctttac
    63721 gttggcgtaa taccaagaaa attaataatg gtgcgcctag tgttgcggta aatgcaccaa
    63781 caggtagtag agtgtcgtaa attctgctaa caatgccgat gagattattg gtaaaaaaga
    63841 gtaaaaatcc gcccgctaga aaagcaatca agaggcgaag taataaatgt cgtattttgg
    63901 caaaatgagc caaacttgca ctgcaaatcc aataaagcca atcacaccta ttttgcttac
    63961 aaccaatgct gtaatggcgg cacaaacaaa aataactaag tagcgtagta aattaacagg
    64021 cgcgcctaag cggcgagctt gttcatcatc taaacttaaa attgtgagtg gacgagagag
    64081 taagacaaag ataaaaaatg caactactac cgttatcgct aaggtaatag aagttgccca
    64141 accatcttgt aaaagtgagc cacttcccca aaccatcacg ccaaataaaa aatcaaagta
    64201 gaaaagggtc agtaaagagg caatagctcc aaacaaaata ttaacaatca atccccctaa
    64261 aataagggta aggggattga gcttagaggc tttagcaata aataaaacca gtacgatagc
    64321 agctaaagag ccaagtaacg caatccagaa actagaaaaa agctcggcgg ctgggaaaaa
    64381 gataaccgca atcatcaatg caagttgagc cccgctactg acggcaagtg tgctatctga
    64441 cgctaaatta tttctgacaa tatgttgtaa gccactgctg gctatagcca ataaaccgcc
    64501 tgctaaaaat gacgttgtca tttcaggtaa aagagctgct tttaccgcag cttgattaat
    64561 tggcaataaa tcaacattta tgagtagtaa ctgccattcc cactcccaga ccatatttaa
    64621 acggataaca gctgttacta aggataaaag aatacagcca atcaataaga atgatgattt
    64681 cattactctt tttctccttg tgtcattgca ttgtgtaaaa actcagcaaa gtgcgagatg
    64741 gaatcagccc caccaaagag ccaaacaggc ggtaatactt tcatacaacg gtttttacca
    64801 aatcctaaac gttgccaaat gtagctattt tctaaatctg cttgcgtcat tttagaaaaa
    64861 ggtgagatga caattaaaca ggtgcgttcg ggtaatttag ctaaatcact cacaggaata
    64921 agtttatttc cccagcgatc tcccacacct aaatcagctt gttttaagcc catcaaatca
    64981 aaggctacac ggaacattga attagacgca tatatgcgta actctctcgg agagccaaat
    65041 tgaacaacag agtagtaatc aatatctgga ttactgttaa ggatttcttt cccccattga
    65101 gccaacgttt gttcagtttt cgttaaatat gcttctgccg cattagggtt tttaatctct
    65161 gttccaatca tacgagtggc tttttggtaa gtatgccaat gttgctgagg ttcagtattg
    65221 ccaccatcaa atagcacgtc tatttgtgga atttctttcg ggtaaacaga agccaagtga
    65281 gcgtagaaaa agtttgatag caccaattca acaggaagct gtgctaataa ttcgcgatta
    65341 ggctgataac gcgctccaat atctaacacg ctattaggct gaatagggcg gtgcgaccat
    65401 agcggataaa ttcgcttatc ccccatcgca atgggcggat ttcccatttc agtaagggca
    65461 gaggcaatag accaatctgg tgtaaaaata ttaggttgag caaatgcggt agaaatttgc
    65521 aaaataaaga gaaaaatgaa ccgcttgtat tgcctaaaca tcataatacc gccactttat
    65581 gtaagcctgc agggtggtta aacaagtgca gttttagccc gaagatctca agtaatttct
    65641 gttcttgcat aatttcatca acagaacctt gaatacagag ttcgccttgc tttaacgcaa
    65701 taatttcatc acaaaattgg gaggcaagat taatatcgtg aataatgata atgacagtta
    65761 gaccttgttc attcactagc ttgcgaataa gctgcattac ttcaacttga taaacaatat
    65821 caagggcagc caatggctca tctagcagta aatagggtgt ttgttgggct aagcacatag
    65881 caagccaaac tcgttgtttt tctccgcctg atagactatt tacaggctgt ttagcaaact
    65941 gagtaacgga ggtgctttcc attgcttgct caacaatttg aacatcaaat tctgttggtt
    66001 tttgtagcca tttttgatat ggaaatcgcc ctaaagcaac cagttcctgc actaagaaat
    66061 ccgccgcttc aggtaaacgt tgtggtaaat aggcaatatg ttttgccgct tctttggtag
    66121 aaagctgatg aagtggtaat ccatcgacta aaattgaacc gctggtaggc gttatatctc
    66181 cgcttagtaa acgcattaac gttgatttac ccgagccgtt atgcgccata taaacgcccc
    66241 ttgcttaaat tgagggaaat attgcttagc agtttacggt tttgttcaac agagtagcta
    66301 atggaattta tttgaatcat agtgttgtgt tttaagagca taaaaaaggt gggattaccc
    66361 caccaaatca atcaaaatga aaatgagtag atgaaaatct attatgaaaa cggtgctaaa
    66421 tatattttta ttataaatga aagtcaatat catttagttt taggtgaggg taaattttag
//
LOCUS       DANLZC010000002        49788 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.002, whole genome shotgun sequence.
ACCESSION   DANLZC010000002 DANLZC010000000
VERSION     DANLZC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 49788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49788
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.002"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            118..621
                     /locus_tag="PXE44_000078"
     CDS             118..621
                     /locus_tag="PXE44_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HDL5587636.1"
                     /translation="MNQSYFNLLGNITWLWMNSSLHKEWSCELLARNVIPAIENEQYM
                     LLIDNGIPIAYCSWADLNLETEVKYIKDINSLTPEEWQSGDRRWIIDWVAPFGHSQLL
                     YKKMCQKYPDMIVRSIRFYPKQKELGKIAYFKGGKLDKKTAKKRFDTYQEELATALKN
                     EFNFIKK"
     gene            637..3498
                     /locus_tag="PXE44_000079"
     CDS             637..3498
                     /locus_tag="PXE44_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX family hemolysin"
                     /protein_id="HDL5587637.1"
                     /translation="MGTRLTTLSNGLKNTLTATKSGLHKAGQSLTQAGSSLKTGAKKI
                     ILYIPQNYQYDTEQGNGLQDLVKAAEELGIEVQREERNNIATAQTSLGTIQTAIGLTE
                     RGIVLSAPQIDKLLQKTKAGQALGSAESIVQNANKAKTVLSGIQSILGSVLAGMDLDE
                     ALQNNSNQHALAKAGLELTNSLIENIANSVKTLDEFGEQISQFGSKLQNIKGLGTLGD
                     KLKNIGGLDKAGLGLDVISGLLSGATAALVLADKNASTAKKVGAGFELANQVVGNITK
                     AVSSYILAQRVAAGLSSTGPVAALIASTVSLAISPLAFAGIADKFNHAKSLESYAERF
                     KKLGYDGDNLLAEYQRGTGTIDASVTAINTALAAIAGGVSAAAAGSVIASPIALLVSG
                     ITGVISTILQYSKQAMFEHVANKIHNKIVEWEKNNHGKNYFENGYDARYLANLQDNMK
                     FLLNLNKELQAERVIAITQQQWDNNIGDLAGISRLGEKVLSGKAYVDAFEEGKHIKAD
                     KLVQLDSANGIIDVSNSGKAKTQHILFRTPLLTPGTEHRERVQTGKYEYITKLNINRV
                     DSWKITDGAASSTFDLTNVVQRIGIELDNAGNVTKTKETKIIAKLGEGDDNVFVGSGT
                     TEIDGGEGYDRVHYSRGNYGALTIDATKETEQGSYTVNRFVETGKALHEVTSTHTALV
                     GNREEKIEYRHSNNQHHAGYYTKDTLKAVEEIIGTSHNDIFKGSKFNDAFNGGDGVDT
                     IDGNDGNDRLFGGKGDDILDGGNGDDFIDGGKGNDLLHGGKGDDIFVHRKGDGNDIIT
                     DSDGNDKLSFSDSNLKDLTFEKVKHNLVITNSKKEKVTIQNWFREADFAKEVPNYKAT
                     KDEKIEEIIGQNGERITSKQVDDLIAKGNGKITQDELSKVVDNYELLKHSKNVTNSLD
                     KLISSVSAFTSSNDSRNVLVAPTSMLDQSLSSLQFARAA"
     gene            3573..5699
                     /locus_tag="PXE44_000080"
     CDS             3573..5699
                     /locus_tag="PXE44_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDL5587638.1"
                     /translation="MEANHQRNDLGLVALTMLAQYHNISLNPEEIKHKFDLDGKGLSL
                     TAWLLAAKSLALKAKHIKKEISRLHLVNLPALVWQDNGKHFLLVKVDTDNNRYLTYNL
                     EQDAPQILSQDEFEACYQGQLILVTSRASVVGQLAKFDFTWFIPAVIKYRKIFLETLI
                     VSIFLQIFALITPLFFQVVMDKVLVHRGFSTLNIITVALAIVIIFEIVLSGLRTYVFS
                     HSTSRIDVELGAKLFRHLLSLPISYFENRRVGDTVARVRELDQIRNFLTGQALTSVLD
                     LLFSFIFFAVMWYYSPKLTLVILGSLPCYILWSIFISPILRRRLDEKFARSADNQAFL
                     VESVTAINMIKAMAVAPQMTDTWDKQLASYVSSSFRVTVLATIGQQGVQLIQKTVMVI
                     NLWLGAHLVISGDLSIGQLIAFNMLSGQVIAPVIRLAQLWQDFQQVGISVTRLGDVLN
                     SPTEQYQGKLSLPEIKGDISFKNIRFRYKPDAPTILNNVNLEIRQGEVIGIVGRSGSG
                     KSTLTKLLQRFYIPENGQVLIDGHDLALADPNWLRRQIGVVLQDNVLLNRSIRENIAL
                     SDPGMPMERVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNN
                     PKILIFDEATSALDYESEHIIMQNMQKICQGRTVILIAHRLSTVKNADRIIVMEKGEI
                     VEQGKHHELLQNSNGLYSYLHQLQLN"
     gene            5711..7147
                     /locus_tag="PXE44_000081"
     CDS             5711..7147
                     /locus_tag="PXE44_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_140441352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDL5587639.1"
                     /translation="MKIWLSGIYEFFLRYKNIWAEVWKIRKELDHPNRKKDESEFLPA
                     HLELIETPVSKKPRLIAYLIMLFLVVAIVLASVSKVEIVATAPGKLTFSGRSKEIKPI
                     ENAIVQEIFVKDGQFVEKGQLLVSLTALGSDADIKKTMASLSLAKLENYRYQTLLTAI
                     EKESLPVIDLSRTEFKDSSEEDRLRIKHLIEEQYTTWQKQKTQKTLAYKRKEAEKQTI
                     FAYVRKYEGATRIEQEKLKDFKALYKQKSLSKHELLAQENKLIEAQNELAVYRSKLNE
                     LENDLLNVKEELELITQFFKSDVLEKLKQHIENERQLRLELEKNNQRRQASMIRAPVS
                     GTVQQLKIHTIGGVVTTAETLMIIVPEDDVLEATALVPNKDIGFVAAGQEVIIKVETF
                     PYTRYGYLTGRIKHISPDAIEQPNVGLVFNATIAIDRKNLTSPDGRKIDLSSGMTITA
                     EIKTGERSVMSYLLSPLEESVTESLRER"
     gene            7388..8386
                     /locus_tag="PXE44_000082"
                     /pseudo
     CDS             7388..8386
                     /locus_tag="PXE44_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            8403..9146
                     /locus_tag="PXE44_000083"
     CDS             8403..9146
                     /locus_tag="PXE44_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5587640.1"
                     /translation="MKQKYIYGLAGLLLLLLGWQLGTALLSQRLPMASMLAPAKTWES
                     FTWLISSGEIFPHLWASLQRIAVGLLAALVIGVPLGLLLGFSKHLEMLCTPSFQLLRM
                     ISPLSWMPIIVMLLGVGDLPIYFLLAFATVWSLIISTTSGVKSIDPQWLQLGRVMSAT
                     RKELLCKIVVPAITGHVLTGLRVAVGIAWVVLVPCEMLGVNQGLGYFILDTRDRLAYS
                     ELMAVIIIIGLIGWALDSLIRLALAKGGR"
     gene            9301..10359
                     /locus_tag="PXE44_000084"
     CDS             9301..10359
                     /locus_tag="PXE44_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDL5587641.1"
                     /translation="MSVLSSSFIQWLQQNAENLDQSNERADLLIPQIAALGLYKIGVP
                     ETLGGSGGTLVDAIKAVTELGNHSLAAAFVSWGHRTFIEHIIASKNPIPRQSYLADLL
                     NGNLTAGTGLSNAMKFLSGIEELNVNIREENGKRYLNGRLPWVTNLRSYRFVASFVAG
                     FQDERKPLVIAIPSTAKGLTRTKELEFIALQGTNTTSLVFENVELQDEWILADDAPEF
                     LAATRPAFLGLQFGLPFGLVERSLAEIEKSLPFRAILASEWQAQVDALNDIKTRLFNG
                     LNEEGYFVRNPKELFQARIDIVNVVAQTILLELQASGGRGYLKNDTSGFSRRWKEAAF
                     LPVVTPSAVQLRMVLANS"
     gene            complement(10480..11022)
                     /locus_tag="PXE44_000085"
     CDS             complement(10480..11022)
                     /locus_tag="PXE44_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL5587642.1"
                     /translation="MSQFQIHTIESAPETAQEALKAVQQANGFIPNLIGVLANAPTAL
                     ETYRTVGGINSRNSLTAEEREVVQITAAVVNGCGFCVAGHTKIALKALKMPEEVVNAL
                     RATARIDDNKLDTLARFTLAVMLQKAKLTEAQLNEFFAAGYNQQNAIDVILGVSLATL
                     CNYVNNIAEIPINPELQPFA"
     gene            complement(11174..12685)
                     /gene="glpK"
                     /locus_tag="PXE44_000086"
     CDS             complement(11174..12685)
                     /gene="glpK"
                     /locus_tag="PXE44_000086"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL5587643.1"
                     /translation="MEKQYIIALDQGTTSSRAVLLDKNANVVEVAQREFTQIYPQAGW
                     VEHNPMEIWSSQSSTLNEVVAKAGIKPDSIAAIGITNQRETTIVWEKETGKPVYNAIV
                     WQCRRTSDITDKLKADGHEEYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAERGEL
                     LFGTVDTWLVWKLTQGRVHVTDYTNASRTMLFNIHTKQWDDKMLELLNIPRSMLPEVK
                     NSSEVYGQTNIGGQGGVRIPVAGIAGDQQAALYGHLCVKEGQAKNTYGTGCFMLMHTG
                     NTAIQSENGLLTTIACNAKGEPEYALEGSVFIAGASIQWLRDELKIVHDSFDSEYFAT
                     KEDDSNGVYVVPAFTGLGAPYWDPYARGSIFGLTRGANRNHIVRATLESIAYQTRDVL
                     EAMQSDSKAKLQALRVDGGATANNFLMQFQADILDTKVERPKVKEVTALGAAYLAGIA
                     VGFWKDLEELKDKAEIERTFTPDGDQEKRAKRYKGWKKAVRRSLEWAKEDAEE"
     gene            complement(12689..13474)
                     /locus_tag="PXE44_000087"
     CDS             complement(12689..13474)
                     /locus_tag="PXE44_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL5587644.1"
                     /translation="MSKSLRNACIGEFIGTGFILFFGAGCVAAAQVAGANFGLWEISI
                     VWGLGVSMAIYISAGISGAHLNPAVTIALAAFYGFEKHKILPYVIAQVAGAFCSVALI
                     YFMYSDLFTAAEAAQGITRGETVGFAGVFSTYPNPNITLVTAFIVEFVITVVLMSTIL
                     AIGDDKNGLPNKALAALLIGLLIAVIGGATGPLTGFAMNPARDFGPKLFAYLAGWGEI
                     ALTGGKEIPYFIIPIVAPICGALFGAWGYKNLIHNNLPANTQE"
     gene            13687..14046
                     /locus_tag="PXE44_000088"
     CDS             13687..14046
                     /locus_tag="PXE44_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDL5587645.1"
                     /translation="MISIGAVLGAMCRWQLAVWLNPILSQFAFGTLAANWLGCLLIGI
                     AMGFNLGESQRLLFISGFLGSFTTFSSFSLELTEKLLAEKWGQFVTVLSLHLIGGLLL
                     TLIGVMLARAVTSGRFC"
     gene            complement(14094..15416)
                     /locus_tag="PXE44_000089"
     CDS             complement(14094..15416)
                     /locus_tag="PXE44_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5587646.1"
                     /translation="MSAMFIIQFAIVLLCILVGARVGGIGLGVFGGLGLAILSFGFGL
                     KPAGLPIDVMFMIMAVVSAAAAMQAAGGLDYMIKIATRILRKNPKYITFIAPLVTWTF
                     TVLAGTGHVAYSVLPVIAEVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAF
                     LEPQGVTLGNVLSVTIPATLLGLGLACVFVNKMGKELKDDPNYQRLLQDPEYVKENHI
                     TTNPTELPLKPTAKLSVGLFLFGALLVVLMGAIPALRPVFDGKPMGMAHTIEIVMLSI
                     GALIIFFCKPDGNEITKGSVFHAGMRAVIAIFGIAWLGDTLMQGHMDEVKSMVSGLVE
                     TAPWAFAFALFVLSVLVNSQGATVATLFPVAIAIGIPAPVLIGVFVAVNGYFFIPNYG
                     PIIASIDFDTTGTTRIGKYIFNHSFMLPGLLSMIFSIGFGLLLSNILL"
     gene            15565..16389
                     /gene="dapF"
                     /locus_tag="PXE44_000090"
     CDS             15565..16389
                     /gene="dapF"
                     /locus_tag="PXE44_000090"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL5587647.1"
                     /translation="MQFSKMHGLGNDFMVVDGVTQNVYLTEEMIRTLSDRHRGIGFDQ
                     LLLVEPPYDPELDFHYRIFNADGSEVSQCGNGARCFARFVTLKGLTNKKDIHVSTAKG
                     KMVLSLQEDGKVRVNMGEPIWEPSQVPFTANKFEKNYILRTELQTVLCGVVSMGNPHC
                     VLQVDDVKTAPVNELGPLLESHERFPERANISFMQVVNRNHVKLRVYERGAGETQACG
                     SGACGTVAVGIMQGLLDNKVQVDLPGGTLIIEWQGVGHPLYMTGDATHIYDGFIKL"
     gene            complement(16560..17102)
                     /locus_tag="PXE44_000091"
     CDS             complement(16560..17102)
                     /locus_tag="PXE44_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDL5587648.1"
                     /translation="MAQVYIVHGYTANADKHWFPWLEQELAKCGVSCERLNMPDSAKP
                     SLDGWLNHLAQNVELTDQTVFVGHSLGCIAILNFLAKNYAKIKGAVFVSGFYQPVENL
                     PELSAFTNYYAILPSVSSFPSYVVSALDDKVVNHQYSDALAQHLHADYIRLNQGGHFL
                     DREGVTELPVVLELVKKLLE"
     gene            17226..17549
                     /locus_tag="PXE44_000092"
     CDS             17226..17549
                     /locus_tag="PXE44_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HDL5587649.1"
                     /translation="MNVWILLAISICLEIAATNLLKLSNGFTKAIPTIGSLALYGLSF
                     YFLSIIFRTLPVGIVYAIWSGVGIVLTALVAYFAFGQKIDLAGLIGISLIIAGVLVIN
                     LFSKV"
     gene            complement(17612..19057)
                     /gene="xylB"
                     /locus_tag="PXE44_000093"
     CDS             complement(17612..19057)
                     /gene="xylB"
                     /locus_tag="PXE44_000093"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDL5587650.1"
                     /translation="MYIGIDLGTSGVKVVLLDENQHIIAITQKSLPIFRPHPLWSEQN
                     PQDWWNATNEAMLELSATQDLQAVKAIGLTGQMHGATLLDNADNVLSPAILWNDGRSF
                     MECEELEQLVPNSREITGNLMMPGFTAPKLRWVDKHQPDIAEKVSKVLLPKDYLRLLM
                     SGEYASDMSDASGTMWLDVGKRNWNKSLLNACGLDIENMPKLFEGNQITGYLRPTLAE
                     QWKMKSVPIVAGGGDNAAGAIGIGLYQTGQAMLSLGTSGVYFVVSDKFQANPQKAVHS
                     FCHALPDRWHLMSVILSAASAVDWAVKSLGLKDVSTLFQQVEASQTASDAIFLPYLSG
                     ERTPHNDPYAKGVFWGLSHNDNQITMAKAVIEGVSFALAEGIEVLHETGVVADNIALI
                     GGGAKSVYWRQLLADISGKTFEYRTSGDVGPALGAAKLAQIALNPNQDIATFCQPLPL
                     EQVYQPTPARYAEYQQKRQKFTELYRRLKGL"
     gene            complement(19210..20529)
                     /gene="xylA"
                     /locus_tag="PXE44_000094"
     CDS             complement(19210..20529)
                     /gene="xylA"
                     /locus_tag="PXE44_000094"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDL5587651.1"
                     /translation="MSNYFDKISKVQYEGANSTNPFAFKHYNPDEVVLGKTMAEHLRL
                     AVCYWHTFCWTGNDMFGVGSLDRSWQKTGDLLQGAKQKAEIAFEFFQKLGIPYYCFHD
                     VDVAPEGNNFKEYQHNFNTIVDILEQKQAETGVKLLWGTANCFTNPRYMSGASTNPNP
                     EVFAWAAAQVFTAMNATKRLGGENYVLWGGREGYETLLNTDLKREREQIGRFMQLVVE
                     HKHKIGFNGTLLIEPKPQEPTKHQYDYDVATVYGFLKQFGLEKEIKVNIEANHATLAG
                     HTFQHEIATATALDIFGSIDANRGDPQLGWDTDQFPNSVEENTLVMYEILKAGGFTTG
                     GFNFDAKIRRQSTDPYDLFHAHIGAIDVLALSLKRAAKMLEDQALQKVVDNRYAGWNQ
                     DLGLQILNGKASLEDLAKIVETQGLDPKPVSGQQEYLENLVNSYIYR"
     gene            20771..21772
                     /gene="xylF"
                     /locus_tag="PXE44_000095"
     CDS             20771..21772
                     /gene="xylF"
                     /locus_tag="PXE44_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5587652.1"
                     /translation="MKLKSKLLAVATATLMVLSNASVAKDLKIGMSIDDLRLERWQKD
                     RDIFVNKAESLGAKVFVQSANGDATAQISQIENMLNKDIDVLVIIPFNGEVLSNVIAE
                     AKKEGVKVLAYDRLINNADVDFYVSFDNEKVGELQAKSIIGQKPEGNYFLMGGSPVDN
                     NAKLFRKGQMKVLQPLIDSGKIKVVGDQWVDSWLAEKALQIMENALTANKNNIDAVVA
                     SNDATAGGAIQALSAQGLSSKVAISGQDADLAAIKRIIAGTQTMTVYKPITNLADKAA
                     EIAVALGKDEKVEMNAQLNNGMKEVPAYLLEPVVVTKENIDDTVIKDGFHTKDAVYK"
     gene            21839..23353
                     /gene="xylG"
                     /locus_tag="PXE44_000096"
     CDS             21839..23353
                     /gene="xylG"
                     /locus_tag="PXE44_000096"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDL5587653.1"
                     /translation="MAKLLEMKNITKKFGEVVALNNISISLETGEILSLCGENGSGKS
                     TLMKVLCGIYPAGDYEGEIYFSGEKLIARNIKDTEEKGISIIHQELTLVKNMSILENM
                     FLGNEITKSGITDDNEMYLRCKTLLEQVQLDIDPNTKVGELGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEKETEILLSLIKDLQAHNIACVYISHKLNEVKAISDKICVIRDG
                     EHIGTRSAEGMSEDDIITMMVGREITSLYPHEPHNIGEEVLRVENLTAWHPTNTHIKR
                     VDNANFVLRQGEILGIAGLVGSGRTEMAQCIFGSYQGKYQGDIFLHKQPIKIKNCAQA
                     IENHIVMVPEDRKKHGIIPIMGVGKNITLSSLPQFCFGKKVINEPLEETIINQSIAKL
                     KVKTSSPELAIGRLSGGNQQKAILAKCLLLNPKILILDEPTRGIDVGAKYEIYKLINQ
                     LAKEGMAIIVISSELPEVLGISDRVLVMHQGKIKADLINQDLTQEKVMEAALKE"
     gene            23357..24484
                     /locus_tag="PXE44_000097"
     CDS             23357..24484
                     /locus_tag="PXE44_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL5587654.1"
                     /translation="MNKLKSINLQVYIMLIAIVVIMSFFSIATDGAYLSARNISNLLR
                     QTSITGILAIGMVFVIISAEIDLSVGSLMGLLGGFAAISNVWWGWPLSLTIIVTLILG
                     VMLGAWSGWWVAYQKVPSFIVTLAGMLAFRGVLVGLTNGTTVSPISNEMTTIGQGYLP
                     DMVGMILGTIGIFCFIVWGSYQRKARQKLNLTVPSMSKDTLKYGLVAIIVLGAIYLLN
                     DYRGVPFPVLVLVILAIAGTFLARKTAFGRHIYAIGGNIDAARLSGISVEKVKLAIFA
                     LNGLLVAIAGLILSSRLGAGAPSAGQNAELDAIAACVIGGASLAGGIGSIYGVVIGAF
                     IIALLDNGMSMLDVPTFWQYIVKGGILLLAVWVDTISKKKA"
     gene            complement(24500..25030)
                     /locus_tag="PXE44_000098"
     CDS             complement(24500..25030)
                     /locus_tag="PXE44_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5587655.1"
                     /translation="MSENSLKTHYSAKELAELSLACLPKTKQGVLYQAKKQGWTTHKK
                     VGVGGEILEFALSNLPQEIQTEIRSRFMSAVVEAKPKKLPAVRAEVDVGNLTTKQREI
                     ADARMALVAYVLELEGSMSRIKAITYLCNLAKQGEMPPHLVELVAVANAKKTAKRTIV
                     PIFSPIMPDFPLDSRD"
     gene            complement(25046..25309)
                     /locus_tag="PXE44_000099"
     CDS             complement(25046..25309)
                     /locus_tag="PXE44_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5587656.1"
                     /translation="MSTLEDIKKTAKDWHRADIIAALHKAGWSLRQLSLKHGYSQSST
                     LKNAVDRPWLKGERIIAEAIGISPEEIWPERYAQRNHKKFADR"
     gene            25493..25975
                     /locus_tag="PXE44_000100"
     CDS             25493..25975
                     /locus_tag="PXE44_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5587657.1"
                     /translation="MKIECKKVIGFGNRIEQSRKNAKLSRSEVCDALGGIAISTLQAW
                     EADTREPPISKLMALSQILNVEPCYLLTGERHSQSLNVTREPQPVYATKPQTEEGEVV
                     VIKAKLQEAIETLEEVLEITQREMKPAGKAQMVWAFYELLTQEKSEKEKMVGLLKLVA
                     "
     gene            25987..26238
                     /locus_tag="PXE44_000101"
     CDS             25987..26238
                     /locus_tag="PXE44_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587658.1"
                     /translation="MDKQAILKLFRDNSTAGNSQPSQEPIQVFNNSNVIIQNGDNATV
                     IAGCTVDNNTNLTCADKQRVHDLIHSLEQRNRASRDEGA"
     gene            complement(26528..28393)
                     /locus_tag="PXE44_000102"
     CDS             complement(26528..28393)
                     /locus_tag="PXE44_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDL5587659.1"
                     /translation="MQSLLSFVPDFLKQELFWVMALLVFAIWQFIQNKLRMDVVALIV
                     MLFFSLTNILSVKEVLAGLSDPNVVLIALLFIVGEGLVRTGVANQVSDWLMRVSGASE
                     TKVLVLLMLCIAGLGSFMSSTGIVAIFIPVVLAICSGMGISPRRLMMPLSVAGLISGM
                     MTLIATPPNLVAHSELVKSGFEGFNFFSFTPIGLIILVMGIGYMLIARRWLDNGEQSN
                     THSQAQKFSMSQLIEEYQLKERAKMVLVEADSDCVGKSIGQLNLRIEQGLNIIAIQRT
                     KRFRKITVSASVNEIFQPKDILLLDTALDDETYQQRCEQLKLRPIELKGEYFSSQYRS
                     VGMAEISVMPEAECIGKTIRELKFRSRYHLSIVGLKRGDEIIQEELLDTPLKFGDILL
                     VMGVWQQIHRMDDDHRDFFLLRDPAESKNAPPALSQAPHALFSVLTMVLLMITGIVPN
                     VMAALIACLMLGKFRCVDMKSAYDSIHMPSIVLIVGMMPFSIALQKTGGVDLTVRLLL
                     DAMQGLDVHFVLIVLFVVTAIISAFISNTATAILVMPIAIAIANQLGYSPAPFVMTVA
                     VSASAAFMTPVSSPVNTMVLAPAGYKFIDFVKVGVPFTVLVMLVSVFVIPMLFPL"
     gene            complement(28533..29048)
                     /locus_tag="PXE44_000103"
     CDS             complement(28533..29048)
                     /locus_tag="PXE44_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HDL5587660.1"
                     /translation="MMDYIDKLSEETTLRGFLSIANQRFAKAVEQLIERVFRKTDFAL
                     KSVVDSLFEHQGPLADIEVRLKVLLGLGVISPKVFEDISLFLEVKQHLNEEEDEFAFS
                     HPAVVKFAKDLHHIDFLPINELLNMKASAANKDSMLFQMQQMRLEKMVRSSLILAVTE
                     IDEQLNVESPL"
     gene            complement(29060..30199)
                     /locus_tag="PXE44_000104"
     CDS             complement(29060..30199)
                     /locus_tag="PXE44_000104"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL5587661.1"
                     /translation="MNALHFGAGNIGRGFIGKLLADARIFVTFADINQTQIDQINQNK
                     QYGVKIVGDDSRVEIVKNIAAINSKDENAVIEQVKNTDLITTAVGPNVLGFIAPLFAK
                     ALVARVESGNTQPLNIIACENMVRGTTFFKGKIFEHLTAEQQAEIEKVVGFVDSAVDR
                     IVPPAEPNPADPLEVTVEEFSEWIVDQTQFKGDIPNIKGMELTDNLMAFVERKLFTLN
                     TGHLICAYLGKQAGVKWIKEAIAIEEIKTQVKATMEESGAVLIKRYGFDPQAHSAYIE
                     KILKRFANPYLNDDVNRVGREPIRKLSENDRLIKPLRGTLEYGLPYQNLVKGVVMALQ
                     YRNEEDPQAVELAEFIANHGVAAAVEKYTGLTDQAVIAQVVELYQ"
     gene            complement(30303..32162)
                     /locus_tag="PXE44_000105"
     CDS             complement(30303..32162)
                     /locus_tag="PXE44_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL5587662.1"
                     /translation="MSNLKVKVQGFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPN
                     ETFAKLVGPMITYLLPLLIGYSGGKLVGGERGAVVGAITTMGIIVGSEIPMFLGAMIV
                     GPLGGWAIKTFDKAIDGKVKSGFEMLVNNFSVGIIGMILAMLSFAFIGGIVTQISAAL
                     GAGVGALVEAGLLPVTSIIVEPAKILFLNNAINHGVFTPLGTAQAQEVGKSILFLIEA
                     NPGPGFGILLAYMFFGRGTAKQTAGSASIIHFLGGIHEIYFPYVLMSPRLILAVIAGG
                     ATGVLTNSLLGGGLIAPASPGSIFAILAMTAPGAHFAVVASVILSCAVTFAIASILLK
                     TQKGEASLEDAQAQTQAMKAESKGVVNGNINKIYVACDAGMGSSAMGASMLRKKVQAA
                     GLNIEVQNLAINDLPQDAQLVITHKDLTARATQRVATAQHLSLTNFLDSAFYDSLVAK
                     LANHQPQQAVQNTQNFANPVEEPQAEGLKLQAAQVFLGLQANSKEEAIRFAGEQLVKA
                     GFVQPSYVDAMFAREQMVSTYLGEGLAVPHGTSDAKDSVLKTGIVVCQYPNGVRFTDE
                     EDGVAKLVVGIAAKGNDHIQILSAITNALDDEQAMKTLTSTNDVEKVLALLSK"
     gene            32464..33014
                     /locus_tag="PXE44_000106"
                     /pseudo
     CDS             32464..33014
                     /locus_tag="PXE44_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005752768.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(33058..33993)
                     /locus_tag="PXE44_000107"
     CDS             complement(33058..33993)
                     /locus_tag="PXE44_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDL5587663.1"
                     /translation="MNHLDSAKETLNLYAQQIITLNHRLSEEFNEVVNMILACQGRVV
                     VGGIGKSGLVGKKMVATFASTGTPSFFLHPTEAFHGDLGMLKPIDMVILISNSGETDD
                     VNKLIPSLKNFGNKIIAMTGNPYSTLGRNADVILNIGVEREACLNNLAPTSSTLVTMA
                     LGDALAIALMKARDFRPEDFARYHPGGSLGRKLLNRVRDVMVRKVPTASLDTTFTECL
                     SVMNEGRMGVAVIMQDDKLEGIITDGDIRRTLAKFGAESLNKTADEIMTRNPKTINDT
                     EFLAKAEDLMKELKIHSLIAVNDEGKVTGLMEFSS"
     gene            complement(34004..34801)
                     /locus_tag="PXE44_000108"
     CDS             complement(34004..34801)
                     /locus_tag="PXE44_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor"
                     /protein_id="HDL5587664.1"
                     /translation="MKPFERRQQILAYLAQHGRTEVDVLADYFKLTGATIRKDLTSLE
                     EDKKVLRTYGSVVLIDEEILEREEKINKEDKDDKPLAQKTGINLQQKQAIGQKAAELI
                     NEGNSIIFDAGSTVLQMIPHLKAFDNLSLMTNSLTIFNEILKLNKSYNLLISGGTFRE
                     KSASFHGYFAESAFIGSNFDILFIGTDGLDLDVGLTTFNEVYKVSSLMCDAANKIVLL
                     ADSSKFGRKSPNIVCGLEKIHTIITDEGLSQEMREQIKAKGIELLVV"
     gene            complement(34844..35491)
                     /gene="hxpB"
                     /locus_tag="PXE44_000109"
     CDS             complement(34844..35491)
                     /gene="hxpB"
                     /locus_tag="PXE44_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDL5587665.1"
                     /translation="MQINSVIFDMDGVMIDSEPQWAEAQIAVLADLGINITEQDCEQL
                     TRGKRIDELSDTWIKKFQLSVSNQALSEAILAHGCAAIEQHGKALTGLYELLDFLKSN
                     QIKLAVATSSPPVVIKAVFDRLKLWDYFPIRCSAMDEEYGKPHPAVYLSAVKKLGIEK
                     QECIVIEDSVTGLIAAKAANLTTFLVNTDYQNEKFSIADKRMASLLDVIEKLKQH"
     gene            complement(35554..35934)
                     /gene="gutM"
                     /locus_tag="PXE44_000110"
     CDS             complement(35554..35934)
                     /gene="gutM"
                     /locus_tag="PXE44_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDL5587666.1"
                     /translation="MDSITLLIILAMIMWLLQAVLGWLQIKAFNQAFMEISKKGKVTV
                     GRNSGRFNPKSVIVLAFDDEKKVVDSLCMKGFSVFARPQKLASVIGLSLDQIVPEKIF
                     PSDKRSQFALKVAISSGYSCSDQK"
     gene            complement(36103..36891)
                     /gene="srlD"
                     /locus_tag="PXE44_000111"
     CDS             complement(36103..36891)
                     /gene="srlD"
                     /locus_tag="PXE44_000111"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDL5587667.1"
                     /translation="MNNEKKVAIVVGGGQTLGAFLSEGLADAGYRVVVADLNGENAQK
                     VAADINAKQGEGNAIGVQVDAADEPSVEALAKATDTVFGRADVLVYSAGTAKASPIWD
                     FNLNDFDLSVKVNLTGYFLTAKHFSRLMIRDGIKGRIIQINSKSGKVGSKYNSGYSAA
                     KFGGVGLTQSLALDLADKGITVHSLMLGNLLKSPMFQSLIPQYAAKLGIPESEVEQVY
                     IDKVPLKRGCDYQDVLNVLKFYAGEEAAYCTGQSINITGGQVMF"
     gene            complement(36939..37337)
                     /gene="srlB"
                     /locus_tag="PXE44_000112"
                     /gene_synonym="gutB"
     CDS             complement(36939..37337)
                     /gene="srlB"
                     /locus_tag="PXE44_000112"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL5587668.1"
                     /translation="MHRLSKRENIYMTIIYQTTFTKIGNFAQESLTDDMLITFKQGAP
                     ADLQDYCFIHNPSELSSPLEVGDIAEFDGVAYPITAVGSVASENLSALGHITFRFDGA
                     NDAEFPGSVHVIGTPPQGLTENSTLIIKRD"
     gene            complement(37382..38383)
                     /locus_tag="PXE44_000113"
     CDS             complement(37382..38383)
                     /locus_tag="PXE44_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL5587669.1"
                     /translation="MSNKAIYIEKGNGGWGGPLTLPVVEGKKVLYMTGGTRPAIVNKL
                     VELTGWEAVDGFKEGEPPAEEIGIAIIDCGGVLRCGLYPKRRIPTINIHSTGKSGPLA
                     EFIVEDIYVSAVKENNIRLIDADGSVPVATEQAKPTSSTTATAKEYDSSKKITEQSDG
                     LLAKIGMGMGSVAALFFQAGRDTIDTVLKTILPFMAFVSALIGIIIASGLGDVIAHGL
                     TPLANSPIGLVTLALICSFPLLSPFLGPGAVIAQVIGVLVGTQIGLGNIPPHLALPAL
                     FAINAQAACDFIPVGLSMAEAKQDTVRVGVPSVLVSRFLTGAPTVLIAWAVSAFIYQ"
     gene            complement(38414..38968)
                     /gene="srlA"
                     /locus_tag="PXE44_000114"
     CDS             complement(38414..38968)
                     /gene="srlA"
                     /locus_tag="PXE44_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725059.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL5587670.1"
                     /translation="MIEAITHGAEWFIGLFQKGGETLVGMINGILPLLISLLVVMNAL
                     IKFVGQDRIERLAQRSAGNPISRYLILPVIGTFVFCNPMTLSLGRFLPERYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLGLPLGPLAISYLLVGMFTNFFRGWI
                     TDFTTAFFEKRMGITLDREVHLSH"
     gene            complement(39206..39514)
                     /locus_tag="PXE44_000115"
     CDS             complement(39206..39514)
                     /locus_tag="PXE44_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL5587671.1"
                     /translation="MKKYIATMLLAFGLVHSSYAQEKTAQIETKQAEKTVVLHIPEMY
                     CQLCVYLVNKELRAVEGVISTKASMKERKATVVVKESVDNQALIQAVGNLKYTAKVIE
                     "
     gene            complement(39516..39884)
                     /locus_tag="PXE44_000116"
     CDS             complement(39516..39884)
                     /locus_tag="PXE44_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587672.1"
                     /translation="MNSSLKSYNHKFITMCVTAIVSAVTSTFCCIGPLVYLVFGISSP
                     WLMELNQLEFLQLPMLLISLAAFGYGFWLLNFSNKIICTKYFSRRTLVILYWVVSVVV
                     MFFLTYPYVLPYILEMWSGE"
     gene            complement(39859..40965)
                     /locus_tag="PXE44_000117"
     CDS             complement(39859..40965)
                     /locus_tag="PXE44_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HDL5587673.1"
                     /translation="MNKLIASLVASSCATAFAAPVQQNNFHTTTHTYEFTQSYDLVVP
                     QGSKGTTNLWIPLPSDTDYQTVKSIEFEGSYKNAYITENNAYGAKTLFATWDTDAPKR
                     DLKVKMVIQTQDREPMAQGALANYQMPEKITYSVDVLEYLKPTAHIKTDGIVKEYADK
                     IVGNEQNPLKKAELIHKWIVDNMERDNSVLGCGDGDVEKILTTKVLKGKCTDINSVFV
                     ALARASNIPAREVFGIRLGQAVKMGQYSKTAFGSSKDGVANESGGQHCRAEFYLAGFG
                     WVPVDSADVAKMRLAEKKDVDDADVQAVSKYLFGNWEMNWVGFNHGRDFDLYPQPELT
                     PLNNFGYPYAEVGGDPLNSFEAKEFGYEFISKEL"
     gene            complement(41205..42248)
                     /locus_tag="PXE44_000118"
     CDS             complement(41205..42248)
                     /locus_tag="PXE44_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL5587674.1"
                     /translation="MQPINIAIAGAGFSSRVFHIPFLKQDARFNIVKVYERTSNNAAE
                     WLPNAETVRDFNALLTPEVDLVLITTPNQTHYAMVKESLLAGKHVLVEKPLVATVAEA
                     EELAELAKRQGKVLYVYQNRRWDSHIATAKSLMEQNLLGELVDCEIRFDRYAKEKNVK
                     VWKESGELGTGLVYDLGVHLIDQALYLFGKPEAVYADVRYQHEGALVDDNFDIQLYYA
                     NGFKVVLKASRYAREPSPTFVLHGKLGSYLKQTPDNQEDLLKNGVQPIGKDWNIEEKE
                     NWGILHTEVEGNVVRQPYRNAEVSYQNLFDDLYQAIAHNAEPIIKLEDVIFVLKVIES
                     VFESAKLGQKIYL"
     gene            complement(42352..42636)
                     /locus_tag="PXE44_000119"
     CDS             complement(42352..42636)
                     /locus_tag="PXE44_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587675.1"
                     /translation="MRYWLISLSILLSGCVVPVTPTGQAGIPMPIIIGVSDPLDSKEV
                     HICTLKPFTQEYRSEHSSRGRAKLNVQKQCRANHSEMFCEEKDIVCKTYD"
     gene            complement(42666..42851)
                     /locus_tag="PXE44_000120"
     CDS             complement(42666..42851)
                     /locus_tag="PXE44_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL5587676.1"
                     /translation="MKCRQATELISQSCDEKLRLQQHMRLQLHLLVCPKCRNFNKNTK
                     KLGKILRAYCNDKDAPF"
     gene            complement(42848..43414)
                     /locus_tag="PXE44_000121"
     CDS             complement(42848..43414)
                     /locus_tag="PXE44_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL5587677.1"
                     /translation="MKSLSSTQIEEIRKQMVKFATLQLADKDLAEDVVQDALTNAYKH
                     SASFRREAALKTWIFAILKNKILDLLRTRKRHIPVSSLCEEEESPSAFFDRHDHWLEH
                     QDVTEWRGVAQSTYQKEFWAIFDICLNKLPAQQARVFMMREHLEMSSAEICQECEISL
                     SNLNVLLHRARLRLQECLSRNWFREESK"
     gene            43523..44476
                     /gene="msrP"
                     /locus_tag="PXE44_000122"
     CDS             43523..44476
                     /gene="msrP"
                     /locus_tag="PXE44_000122"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDL5587678.1"
                     /translation="MKKLTIQDVTPETIFYQRRKIITALGLGIAASALPKVSMAETQR
                     HLQSLQFKPDPNKGLELTPENKVIGYNNFYEFGVDKSAPAKNAANFKTDPWTLEIGGE
                     VENPFSLNHQQLLNTFPLEERIYRFRCVEAWSMVVPWIGFELARLIEMAKPTSKAKYV
                     VFHTLYDPAQMPGQKNRLLGGGIDYPYVEALRLDEALNSLTLLSVGLYGKTLPPQNGA
                     PIRLVVPWKYGFKSIKSIVKITFAEKRPLTTWESLAPNEYGFYANVNPNVDHPRWSQA
                     SERVIGAGGLLSVKRQPTLMFNGYENEVANLYRGLDLKVNY"
     gene            44476..45060
                     /locus_tag="PXE44_000123"
     CDS             44476..45060
                     /locus_tag="PXE44_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
                     /protein_id="HDL5587679.1"
                     /translation="MLTWLRILIHLGCLLPLVWVAQLLYTGNETILGADPIKELEHFL
                     GYGAIVIFCIMFLLGIALQYLKKNQYQILRRPLGLWAFVWAALHISSYLLLELSLDVK
                     LFFSELAGRPYLILGAAAFFILSIMAITSLPMLKRKLGTRWGTIHQWAYPALVLAMVH
                     YYWSVKSVTFDPIIIGVLVLFIVAHKIQAKFAKK"
     gene            45157..45807
                     /locus_tag="PXE44_000124"
     CDS             45157..45807
                     /locus_tag="PXE44_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha2 family transposase"
                     /protein_id="HDL5587680.1"
                     /translation="MRKSRLSQYKQHKLIELFVAGVTARTAAELVNVNKTTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEADESYFGGARKGKRGCGAAGKTAVFGLLKRDDKVYTVV
                     VPNTQSATLLPIIREKVKPDSLVYADFYRSYDVLDVSEFNHFRINHSTHFAENHNHIN
                     GIENFWSQAKRHLRKFNGIPKAHFELYLKECEWRFNHSDLKSQIFLLKQLVKGGLG"
     gene            complement(46006..46245)
                     /locus_tag="PXE44_000125"
                     /pseudo
     CDS             complement(46006..46245)
                     /locus_tag="PXE44_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
     gene            complement(46500..46994)
                     /locus_tag="PXE44_000126"
     CDS             complement(46500..46994)
                     /locus_tag="PXE44_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5587681.1"
                     /translation="MSRKYKFHEKSGAYFVSFATVYWVGVFVREIYFQVIVDALDYCR
                     KHKGMILYAYCIMPTHIHLLFQAENQNPSDLIRDFKTFTAKILIQRIKDNLQENRREW
                     LLWMFSRAAERRCNVKQYQFWQHHNQPIEIYSQKFFDEKLNYIHQNPVVSGFVSEPWE
                     WKYN"
     gene            complement(47598..48437)
                     /locus_tag="PXE44_000127"
     CDS             complement(47598..48437)
                     /locus_tag="PXE44_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5587682.1"
                     /translation="MGEFNCPDVNFPHTATGKHKRINYSATLSAYIHDLFTPFISYSQ
                     THRAPNIKEIFFSNLGDYGVNTALQPEQAKTTQFGINGFKERVFSEEDKLGFKVLFYN
                     THLKNYIYNVQHTAVGSAPSLFIFHKNYEKPVNIKGIELEFSYDIGSFYGNLAYARQN
                     TNQPVSFTDASSRVDAASHYGFKTQGFGASKISALPRDYGSLELGTRWFDQRLQIGGV
                     AKYYGKSKRIAIDEFNYTYYPGTHIVKETHRLEETVNKQPIIVDFYVSYEPIKSLILK
                     LEL"
     gene            complement(49104..49481)
                     /locus_tag="PXE44_000128"
     CDS             complement(49104..49481)
                     /locus_tag="PXE44_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5587683.1"
                     /translation="MDKSQGVVSVNIRGGSGFGRANNAVGPNYMGMLAGKYQFNDSTW
                     IGALFGHSWRKISQNYKIGGGRKVTDSSIDLSKIDDLDKESSTTSPFNAAHLKQKPMS
                     RMAKLEYGDKYQTITLLPKSGSG"
BASE COUNT        14846 a        10348 c        10026 g        14568 t
ORIGIN      
        1 caatttaagc tacaaaaaac aacaaaaaac aacaaaaaac acgacaataa gatcgagtaa
       61 tgattatatt atgttataat ttttgaccta atttagaata attatcgagt gcaaattatg
      121 aatcaatctt attttaactt actaggaaac attacttggc tatggatgaa ctcctccctc
      181 cacaaagaat ggagctgtga actactagca cgcaatgtga ttcctgcaat tgaaaatgaa
      241 caatatatgc tacttataga taacggtatt ccgatcgctt attgtagttg ggcagattta
      301 aaccttgaga ctgaggtgaa atatattaag gatattaatt cgttaacacc agaagaatgg
      361 cagtctggtg acagacgctg gattattgat tgggtagcac cattcggaca ttctcaatta
      421 ctttataaaa aaatgtgtca gaaataccct gatatgatcg tcagatctat acgcttttat
      481 ccaaagcaga aagaattagg caaaattgcc tactttaaag gaggtaaatt agataaaaaa
      541 acagcaaaaa aacgttttga tacatatcaa gaagagctgg caacagcact taaaaatgaa
      601 tttaatttta ttaaaaaata gaaggagaca tcccttatgg gaactagact tacaacccta
      661 tcaaatgggc taaaaaacac tttaacggca accaaaagtg gcttacataa agccggtcaa
      721 tcattaaccc aagccggcag ttctttaaaa actggggcaa aaaaaattat cctctatatt
      781 ccccaaaatt accaatatga tactgaacaa ggtaatggtt tacaggattt agtcaaagcg
      841 gccgaagagt tggggattga ggtacaaaga gaagaacgca ataatattgc aacagctcaa
      901 accagtttag gcacgattca aaccgctatt ggcttaactg agcgtggcat tgtgttatcc
      961 gctccacaaa ttgataaatt gctacagaaa actaaagcag gccaagcatt aggttctgcc
     1021 gaaagcattg tacaaaatgc aaataaagcc aaaactgtat tatctggcat tcaatctatt
     1081 ttaggctcag tattggctgg aatggattta gatgaggcct tacagaataa cagcaaccaa
     1141 catgctcttg ctaaagctgg cttggagcta acaaattcat taattgaaaa tattgctaat
     1201 tcagtaaaaa cacttgacga atttggtgag caaattagtc aatttggttc aaaactacaa
     1261 aatatcaaag gcttagggac tttaggagac aaactcaaaa atatcggtgg acttgataaa
     1321 gctggccttg gtttagatgt tatctcaggg ctattatcgg gcgcaacagc tgcacttgta
     1381 cttgcagata aaaatgcttc aacagctaaa aaagtgggtg cgggttttga attggcaaac
     1441 caagttgttg gtaatattac caaagccgtt tcttcttaca ttttagccca acgtgttgca
     1501 gcaggtttat cttcaactgg gcctgtggct gctttaattg cttctactgt ttctcttgcg
     1561 attagcccat tagcatttgc cggtattgcc gataaattta atcatgcaaa aagtttagag
     1621 agttatgccg aacgctttaa aaaattaggc tatgacggag ataatttatt agcagaatat
     1681 cagcggggaa cagggactat tgatgcatcg gttactgcaa ttaataccgc attggccgct
     1741 attgctggtg gtgtgtctgc tgctgcagcc ggctcggtta ttgcttcacc gattgcctta
     1801 ttagtatctg ggattaccgg tgtaatttct acgattctgc aatattctaa acaagcaatg
     1861 tttgagcacg ttgcaaataa aattcataac aaaattgtag aatgggaaaa aaataatcac
     1921 ggtaagaact actttgaaaa tggttacgat gcccgttatc ttgcgaattt acaagataat
     1981 atgaaattct tactgaactt aaacaaagag ttacaggcag aacgtgtcat cgctattact
     2041 cagcagcaat gggataacaa cattggtgat ttagctggta ttagccgttt aggtgaaaaa
     2101 gtccttagtg gtaaagccta tgtggatgcg tttgaagaag gcaaacacat taaagccgat
     2161 aaattagtac agttggattc ggcaaacggt attattgatg tgagtaattc gggtaaagcg
     2221 aaaactcagc atatcttatt cagaacgcca ttattgacgc cgggaacaga gcatcgtgaa
     2281 cgcgtacaaa caggtaaata tgaatatatt accaagctca atattaaccg tgtagatagc
     2341 tggaaaatta cagatggtgc agcaagttct acctttgatt taactaacgt tgttcagcgt
     2401 attggtattg aattagacaa tgctggaaat gtaactaaaa ccaaagaaac aaaaattatt
     2461 gccaaacttg gtgaaggtga tgacaacgta tttgttggtt ctggtacgac ggaaattgat
     2521 ggcggtgaag gttacgaccg agttcactat agccgtggaa actatggtgc tttaactatt
     2581 gatgcaacca aagagaccga gcaaggtagt tataccgtaa atcgtttcgt agaaaccggt
     2641 aaagcactac acgaagtgac ttcaacccat accgcattag tgggcaaccg tgaagaaaaa
     2701 atagaatatc gtcatagcaa taaccagcac catgccggtt attacaccaa agataccttg
     2761 aaagctgttg aagaaattat cggtacatca cataacgata tctttaaagg tagtaagttc
     2821 aatgatgcct ttaacggtgg tgatggtgtc gatactattg acggtaacga cggcaatgac
     2881 cgcttatttg gtggtaaagg cgatgatatt ctcgatggtg gaaatggtga tgattttatc
     2941 gatggcggta aaggcaacga cctattacac ggtggcaagg gcgatgatat tttcgttcac
     3001 cgtaaaggcg atggtaatga tattattacc gattctgacg gcaatgataa attatcattc
     3061 tctgattcga acttaaaaga tttaacattt gaaaaagtta aacataatct tgtcatcacg
     3121 aatagcaaaa aagagaaagt gaccattcaa aactggttcc gagaggctga ttttgctaaa
     3181 gaagtgccta attataaagc aactaaagat gagaaaatcg aagaaatcat cggtcaaaat
     3241 ggcgagcgga tcacctcaaa gcaagttgat gatcttatcg caaaaggtaa cggcaaaatt
     3301 acccaagatg agctatcaaa agttgttgat aactatgaat tgctcaaaca tagcaaaaat
     3361 gtgacaaaca gcttagataa gttaatctca tctgtaagtg catttacctc gtctaatgat
     3421 tcgagaaatg tattagtggc tccaacttca atgttggatc aaagtttatc ttctcttcaa
     3481 tttgctagag cagcttaatt tttaatgatt ggcaactcta tattgtttca cacattatag
     3541 agttgccgtt ttattttata aaaggagaca atatggaagc taaccatcaa aggaatgatc
     3601 ttggtttagt tgccctcact atgttggcac aataccataa tatttcgctt aatccggaag
     3661 aaataaaaca taaatttgat cttgacggaa aagggctttc tttaactgct tggcttttag
     3721 ctgcaaaatc gttagcgttg aaagcgaaac acattaaaaa agagatttcc cgcttacact
     3781 tggtgaattt accggcatta gtttggcaag ataacggtaa acatttttta ttggtaaaag
     3841 tggataccga taataaccgc tatttaactt acaatttgga acaagatgct ccacaaattc
     3901 tgtcacaaga cgaatttgaa gcctgctatc aagggcagtt aattttggtc acgtccagag
     3961 cttccgtagt aggtcaatta gcaaagttcg atttcacctg gtttattccg gcggtgatca
     4021 aataccgaaa aatctttcta gaaaccttga ttgtttcgat ctttttgcaa atttttgccc
     4081 taattacacc gctattcttc caagttgtta tggataaagt actggtgcat cgaggttttt
     4141 caaccttgaa tatcattacg gttgccttag ctattgtgat catctttgaa attgtactaa
     4201 gtggtttgag aacctatgtt ttttctcata gcactagccg tattgatgtt gaattaggcg
     4261 ctaaattatt tcgacattta ttatcactac ccatttctta ttttgaaaac agacgagttg
     4321 gagatacagt cgctagggtt agagaattag atcaaattcg taatttcctt accggacaag
     4381 cattaacctc ggtgttagat ctcttattct cttttatctt ttttgccgta atgtggtatt
     4441 acagcccaaa attaaccttg gtaattcttg gttcattgcc ctgctatatt ttatggtcaa
     4501 tttttattag tccgatttta agacggcgtt tagatgagaa atttgcccga agtgctgata
     4561 accaagcatt cttagttgag tcggtaacag ccatcaatat gattaaagcg atggcggttg
     4621 ctccacaaat gacggataca tgggataaac agctggcaag ctatgtttca tcaagtttcc
     4681 gtgtcaccgt attagcaacc attgggcaac aaggtgtaca acttattcaa aaaaccgtta
     4741 tggtgattaa cctttggtta ggggcacact tagttatttc aggcgatctg agtattgggc
     4801 aattaattgc ctttaatatg ctatcagggc aagtgattgc accggtgatt cggctggctc
     4861 agctctggca agatttccaa caagttggga tttccgtcac tcgcttaggt gatgttttaa
     4921 actctccaac cgaacaatat caaggcaaat tatcactacc agaaataaaa ggcgatatct
     4981 catttaaaaa tatccgcttt agatataaac cagatgcacc aactatttta aataatgtga
     5041 atttagaaat taggcaagga gaagtgattg ggattgttgg acgttccggt tcaggcaaaa
     5101 gtactctgac taaattactg caacgttttt atattcctga aaatgggcag gttttgattg
     5161 atggacatga tctagcctta gctgatccaa actggctacg ccgtcaaata ggtgtagtgc
     5221 tgcaagataa tgtgttatta aaccgcagta tccgagaaaa tattgcgcta tcagatccag
     5281 gaatgccaat ggagcgagta atttatgcag caaaattagc aggggctcac gattttattt
     5341 cagaattgcg tgaaggttat aacaccattg tgggtgaaca aggagcgggg ctttcaggcg
     5401 ggcaacgcca acggattgcg attgctcgag ctttggtaaa caacccgaaa atcctgattt
     5461 ttgatgaggc aaccagtgcc ctcgattacg aatctgagca tattattatg caaaatatgc
     5521 aaaaaatatg ccaaggcaga accgtgattt tgattgcaca tcgtttatcg accgtcaaaa
     5581 atgcggatcg aattattgtg atggaaaagg gggaaattgt tgagcaaggc aagcaccacg
     5641 aattactgca aaacagtaac ggactttatt cctacttaca ccaattacaa cttaattaag
     5701 aaggaaaaca atgaaaatat ggcttagtgg tatttatgaa tttttcctac gctataaaaa
     5761 catttgggca gaagtatgga aaattcgtaa agaattagac cacccaaaca gaaaaaaaga
     5821 cgaaagtgaa tttttaccgg cacatttaga actgattgaa accccggttt ctaaaaaacc
     5881 acgtctaatt gcttatttga ttatgctatt tttagttgtg gcaattgtgc ttgccagtgt
     5941 aagcaaagtt gaaattgtgg cgactgctcc cggtaaatta acttttagtg gcagaagtaa
     6001 agaaattaaa ccgattgaaa acgccattgt acaagaaatt ttcgttaaag atgggcagtt
     6061 tgtggaaaaa gggcaattat tagtcagctt aactgcattg ggttctgatg cagatatcaa
     6121 aaagaccatg gcttcacttt ctttagctaa actggagaac tatcgctacc aaactttgct
     6181 tactgccatt gaaaaagagt ccttgccggt gattgattta tctagaaccg aatttaaaga
     6241 ttcatcggaa gaagatcgac tacgtattaa acacttaatt gaggagcaat acaccacttg
     6301 gcaaaaacaa aaaacacaga aaactttagc gtataagcgt aaagaggctg aaaaacaaac
     6361 aatatttgcc tatgtccgta aatatgaagg tgcaacacgt attgaacaag aaaaattaaa
     6421 agactttaag gcactttata aacagaagtc tttatctaag cacgaacttc ttgcgcaaga
     6481 aaataaatta attgaggctc agaatgagct agctgtttat cgctcaaaat taaatgaatt
     6541 agaaaatgat ctactcaatg taaaagaaga acttgaattg atcacgcaat tctttaaaag
     6601 cgatgtgttg gaaaaattaa agcaacatat tgaaaatgaa cgccaacttc ggctcgagtt
     6661 agaaaaaaat aatcaacgca gacaggcctc gatgatcaga gcaccggttt ccggtacggt
     6721 tcagcaactg aaaattcaca ctataggtgg tgttgttacg actgctgaaa ccttgatgat
     6781 cattgtgccg gaagacgatg tgttagaggc caccgctctg gttccaaaca aagatatcgg
     6841 ctttgttgca gcagggcagg aggtgattat taaagtggaa actttccctt atacacgcta
     6901 tggttatcta actggtcgaa ttaaacatat tagcccggat gcgattgaac aacctaatgt
     6961 aggcttagtt tttaatgcaa ctatagctat agataggaag aatctaacat cgcctgatgg
     7021 gcgaaaaatt gatttgagtt caggtatgac aataactgct gaaatcaaaa ccggtgaacg
     7081 gagtgtaatg agttatttac tcagcccatt agaagaatct gtcacagaaa gtttaaggga
     7141 acgctaatcg aaccaaaaca aagccataaa agccattttg agcttttatg gctttatttt
     7201 ttagtccaca agcggtcaaa aaagcccaat tttttacact tttataacaa attgttctaa
     7261 ctaaaaatta ctaattcttt tcttttataa gacgaatcct ctattttcat ttcattaaca
     7321 ttgaactaga aaaggggatt tatgagccta agcattacga atctttctct tggctaccgc
     7381 aaaaatcagc aaaggcttat ttgaaaagca cggtgtgcag gtggaaaaac cggtgatgtt
     7441 tcgcagctgg gctcagttgg tggaagcctt tttaagtggc aatgtgaacg tggtgcatct
     7501 gctttcgcct atgagtttgt gggcgaaata tggagcaaat gctccggtga aagcggtaat
     7561 gtggaatcac ttggcaggtt cggctttaac ggttcgccct gaaatcaaca gtattgccga
     7621 actctccggc aaaacggtag caattccgtt ttggtattca attcataata tcgttttgca
     7681 acgattatta cgagaaaatg gcttgaaaat caccgaaaaa gagccgaaaa gcggtgaagt
     7741 gcgattaagt gtaatggctc cgtcagatat ggtggcagcg ttggcaagtg aggcaatttc
     7801 cggctttatt gtggcagagc cgttcaatgc gttagcggaa gccaaaggtg tggggaaggt
     7861 gctgcgtttt agtggcgatg tgtggaaaga tcacgcctgc tgtttaaccc taatgcacga
     7921 atacgatatt caacaccgcc cggaatgggt gcaaaaagtg gtgaatgtct taaccgaagc
     7981 ccaagtgtcc acgcttgcca accgtgcaga aaccgctcaa ttattggctc ggggcagcgg
     8041 ctacacgccg cacgatcaaa aagtgctgga aaaggtactg gctccaaccc aagctcagtg
     8101 ggataaatat atcgaaacag gggcaattca aaaccctcag tggcaccaac agcgaatcgg
     8161 tttccaaccg tatccgttcg attcctatat ggaagagctg gtcaaaatgc tcaaacagac
     8221 ccacattgcc ggcaataatg cgtttttaca acagcttgac ccggtagagg cagcgaaagc
     8281 gattaacgag ccgacctttg tgcgaaaagc gattgagcaa ggcggttggg ttgaaacctt
     8341 taatttacaa accggttgga caagaaccga gcaaattagt gtataagcga taggaacgaa
     8401 cgatgaaaca gaaatatatt tacggcttag ccggtttatt actgctactt ttaggttggc
     8461 agcttggcac agccttgtta agccaacggt tgccgatggc gtcaatgtta gctccggcaa
     8521 aaacctggga aagttttact tggctgattt cctccggcga aatttttccg cacttatggg
     8581 cgagtttgca acgcattgct gtcgggctac tcgctgcgtt agtgattggt gtaccgctgg
     8641 gcttattgct cggcttctcc aagcaccttg aaatgctttg cacgccgtca ttccaactac
     8701 tacgtatgat ttcgccactt tcgtggatgc cgattatcgt gatgttactt ggtgtaggcg
     8761 atttgccgat ttatttttta ctggcgtttg ccaccgtttg gtcgttgatt atttccacca
     8821 cctcaggggt aaaaagcatt gatccacaat ggttacaact gggtagagta atgtcggcta
     8881 cccgcaaaga actgctgtgc aaaattgttg taccggctat taccggacac gttctcaccg
     8941 gtttacgtgt ggcggttggg attgcgtggg tggtgctggt accgtgcgaa atgctaggcg
     9001 tgaatcaagg actcggctat tttattttag acacccgaga ccgattagcc tattccgaac
     9061 tgatggcggt aattataatt atcggcttaa tcggctgggc gttggatagc ttaatccgct
     9121 tggctttagc gaaaggcggc agataaattg acaagcggtc gaaaaattgc atttttttgc
     9181 aaaaaatcga ccgcttgttt attcccaaaa agcatattac cttaatttat tttatttcta
     9241 gggcataaaa aagcgattta agatagcgac aaagtaaact aattatacga ggtaaacaca
     9301 atgtcggttc tttcatcttc ttttattcaa tggcttcagc agaacgccga aaatttagat
     9361 caatctaacg aacgagcgga tttactcatt ccacaaattg ccgcactagg gctatataaa
     9421 atcggcgtgc cggaaacgct tggcggcagc ggtggcactt tggttgatgc gatcaaagcc
     9481 gttaccgaac tgggtaatca ctcgctggct gcggcatttg tttcgtgggg acatcgtacc
     9541 tttatcgagc atattatcgc ctcaaaaaat cctattcccc gccaaagcta tttggcggat
     9601 ctgctaaatg gcaatttaac cgcaggaaca ggtttatcca atgcgatgaa atttctctcc
     9661 ggcattgaag agttgaatgt gaatattcgt gaagaaaacg gtaaacgcta tttaaatggc
     9721 cgcctgcctt gggtaaccaa tttacgttca taccgctttg ttgctagttt cgttgccggt
     9781 tttcaagacg aacgcaaacc gctagtgatt gcaattccct ccaccgccaa aggcttaacc
     9841 cgcaccaaag aattggaatt tatcgcattg caaggcacga atactacctc tctcgtattt
     9901 gagaatgtgg aattacaaga tgaatggatt ttggctgatg acgcgcctga gtttttagca
     9961 gccactcgcc ctgcgttctt aggtttacaa tttggtttgc cctttggctt ggttgaacgt
    10021 agtttggcgg aaatcgaaaa atcgctccct ttccgtgcaa ttctcgcctc tgagtggcaa
    10081 gctcaagttg atgccctcaa cgatattaaa actcgtctgt ttaatggctt aaatgaagaa
    10141 ggttattttg tgcgtaatcc gaaagagctt ttccaagcga gaattgacat tgtcaatgtc
    10201 gtcgcccaaa ctattctgct agaactgcaa gcgagtggcg gacgtggcta tctgaaaaat
    10261 gatacttcag gcttcagccg ccgttggaaa gaagcggcat ttctgcccgt tgttacgcca
    10321 agtgcggtac agctgagaat ggtgctggcg aatagttaag cggataagag acaaattgtt
    10381 gcctctttta ttttgcaaaa aatagtctaa atataaccgc ttgcaataaa aataaaaaag
    10441 gatgcatttt ttatgcttcc tcaattttac ggctatctat tatgcaaaag gctgcaattc
    10501 agggttaatt ggaatttcgg caatgttgtt aacgtagtta cataatgttg ccaagctcac
    10561 gcctaaaatc acatcaatcg cattttgttg gttgtaacct gcggcaaaaa attcgtttaa
    10621 ttgggcttcg gttaatttgg ctttttgtaa cattactgca agggtgaagc gagccagagt
    10681 gtctaatttg ttatcgtcta tgcgagccgt ggcacgtaaa gcatttacca cttcttcagg
    10741 catttttaat gcttttaagg cgattttagt atgacctgcc acgcagaaac cacagccgtt
    10801 gaccactgct gcggtgattt gtaccacttc acgttcttca gcggttaagc tgttacgact
    10861 attaatacca cccacagtgc ggtaagtttc cagtgctgtt ggtgcgttgg ctaatacgcc
    10921 gatcagattt gggataaaac cgtttgcttg ttgcactgct tttagggctt cttgtgccgt
    10981 ttcaggagcg gattcaatag tgtgaatttg aaattgagac ataaatgttc cttatcaggt
    11041 tgtttattgg tgtggctcat cataaagaaa aggctaattt agaacaaatc aaaatcacta
    11101 tttaatataa ctaaaagatc ttaaagggag tatagttgcc tatactccct ttgtttatgc
    11161 acaggttgcg tttttactct tctgcgtctt cttttgccca ttctaatgag cggcgaacgg
    11221 cttttttcca acctttatag cgtttcgccc gtttttcttg gtcgccgtcc ggtgtgaagg
    11281 tgcgttcaat ttccgctttg tctttcagtt cttccaaatc tttccagaag ccgacagcaa
    11341 tacccgcaag ataagctgcc cctaaagcag tgacttcttt cactttcgga cgctcaactt
    11401 tggtgtctaa aatatcggct tggaactgca ttaagaagtt gtttgcggtt gcaccaccgt
    11461 ccacacgtaa cgcttgtagt ttcgctttgg agtcagactg cattgcttct aacacatctc
    11521 gggtttggta agcgatagac tctagggtcg cacgcacaat gtggttacgg tttgccccac
    11581 gggttaaacc gaaaattgag cctcgtgcat acggatccca atatggagca cctaagccgg
    11641 taaaggcagg tacaacataa acaccgttag agtcatcttc tttagtggca aaatattcgc
    11701 tgtcaaagct atcgtgaaca attttcagct catcacgcag ccattgaata gacgcaccgg
    11761 caataaatac tgaaccttcc aacgcatatt ccggctcgcc ttttgcgtta cacgcaatgg
    11821 tcgttaataa accgttttca gattgaatcg cagtgttacc ggtgtgcatc aacataaaac
    11881 agcctgtacc ataggtattc ttcgcttgtc cttctttcac gcataaatga ccgtagagag
    11941 cggcttgttg gtcacctgca atacccgcga ccggaatacg cacaccgcct tgaccgccga
    12001 tattagtttg cccgtaaact tctgatgaat ttttcacttc aggtaacatt gagcgtggaa
    12061 tattgagcaa ttccagcatt ttatcgtccc attgtttggt gtgaatgtta aacaacatag
    12121 tacgagaagc gttggtgtaa tcggtaacgt gtacccgccc ttgggttaat ttccacacca
    12181 gccaagtatc gaccgtaccg aacagcaatt cgccacgttc tgctttttct cttgcccctt
    12241 ctacgttatc taaaatccac ttcactttag tgccggagaa gtacggatcg actaccaagc
    12301 ctgtcgtttt acgaatatat tcctcgtgtc cgtctgcttt taatttatcg gtaatatccg
    12361 atgtgcgacg acattgccac acaatcgcat tataaacagg tttaccggtc tctttttccc
    12421 acacaatcgt ggtttcacgt tggtttgtga tcccgattgc ggcaatgcta tccggcttaa
    12481 taccggcttt tgccaccact tcatttaagg tggaactttg gcttgaccaa atttccatcg
    12541 ggttatgttc tacccagcct gcttgcggat aaatctgggt aaattcacgc tgagccactt
    12601 caaccacatt ggcattttta tctaataaaa ccgcacgaga gctggttgta ccttggtcga
    12661 gagcgatgat atattgtttt tccatagatt actcctgagt atttgctgga agattgttat
    12721 gaatgaggtt tttataaccc caagcaccga ataatgcacc gcagattggg gcgacaatcg
    12781 gaatgatgaa atacggaatt tcttttccgc ctgttaaagc aatttcaccc caaccggcaa
    12841 gataagcaaa gagtttcgga ccaaaatcac gagccgggtt catcgcaaag ccggttaacg
    12901 ggccggttgc tccaccaatt accgcaatca ataaaccgat gagtaatgcg gctaaggctt
    12961 tattcggcaa gccgttttta tcatcgccaa tcgctaaaat agtggacatc aacaccacag
    13021 taatcacaaa ttcaacgatg aaggcggtaa ccaatgtaat attagggttt ggataggtgg
    13081 agaacacacc ggcaaagcca acagtttcgc cccgagtaat gccttgagcg gcttcggcgg
    13141 cagtaaagag atcgctatac ataaaataaa tcagtgcgac agagcaaaac gctccggcaa
    13201 cttgggcgat gacatagggc aaaattttat gcttttcaaa gccgtagaat gcggcaagtg
    13261 ctatagtgac cgcagggtta aggtgagcac cggaaatacc ggcagaaatg tagattgcca
    13321 tggaaacacc aagcccccaa acgatggaaa tttcccataa accgaaattc gcaccggcta
    13381 cctgagcagc agcaacacaa ccagcaccaa agaaaagaat aaaacctgta ccgataaatt
    13441 cgccaataca ggcattacgt aaggatttgc tcataaagtt tctccttaag atgaaaatga
    13501 ttggaaagtg atgaaaaaat gtttgaatat gaacatattt cggagtgata ttgccaaagc
    13561 ggcatttttt aagcaaatag gaagtttcaa atttgtgaaa tccatcacat tatttttcta
    13621 aatcactcat ttttaggtat gatgtgagaa aacgttagat gaggaaaaat gatgtcttca
    13681 atacttatga tttcaatcgg ggcggtactt ggagctatgt gccgctggca attagccgtt
    13741 tggctaaacc cgatactcag ccaattcgcc tttggtactt tagcagcaaa ttggcttggt
    13801 tgtttgttaa ttggtattgc aatggggttt aatttaggcg agagccagcg gttgctgttc
    13861 atcagcggtt ttttaggcag ttttaccacc ttttccagtt tctctttaga actaacggaa
    13921 aaactattag cagaaaaatg ggggcagttt gtgacagtgc tgagtttaca tttaatcggt
    13981 gggcttttgc ttactttgat aggtgtaatg cttgcaagag ccgttacaag cggtcgtttt
    14041 tgctgaaaat tttgcaaata aaaaacctcc caaacgggag gttttgcgag cgattaaagt
    14101 aggatattgg aaagtaataa accaaatccg atactgaaaa tcatactaag taagcccggt
    14161 aacataaagc tatggttgaa aatatattta ccgatacggg ttgtgccggt ggtgtcgaag
    14221 tcaatcgagg caataatcgg gccgtagttc ggaatgaaga agtagccgtt taccgccaca
    14281 aataccccga ttagcaccgg tgcaggaatg ccgatagcaa tcgctacagg gaataaggtt
    14341 gcaaccgttg ccccttggct gtttaccaat acggaaagta cgaataatgc gaaagcaaat
    14401 gcccaaggtg cggtttcaac taagcctgaa accattgatt tcacttcgtc catatgacct
    14461 tgcattaagg tatcgcctaa ccaagcaata ccgaaaatcg caatcactgc acgcataccg
    14521 gcgtggaaga cagaaccttt ggtgatttca ttaccgtccg gtttgcagaa gaagataatc
    14581 aacgcaccaa tacttaacat cacaatttca atcgtgtgag ccatccccat cggcttacca
    14641 tcaaacacag ggcgaagagc tggaattgcg cccattaata ccactaataa tgcaccgaat
    14701 aggaataagc ctactgataa tttcgcagtc ggttttaacg gtaattcggt tggattggta
    14761 gtaatgtgat tttctttcac atattcaggg tcttgcaata agcgttgata atttgggtcg
    14821 tcttttaatt ctttgcccat tttatttaca aacacacaag ctaaacctaa gcccaataag
    14881 gtcgcaggaa tagtcacact taacacgtta cccaatgtaa caccttgagg ttcaaggaag
    14941 gcaaccacag caactactgc cgcagcaatc gggctggcga caatcgcaaa ttgtgaggcg
    15001 attactgcca ttgaaagtgg acgctcaggg cggataccgt tgtggcggct tacctctgca
    15061 attacaggta atacagaata agcaacgtga cctgtaccgg caagcacggt gaatgtccaa
    15121 gttacaagcg gtgcgataaa ggtaatgtat ttcgggtttt tacgcaaaat gcgagtggca
    15181 attttgatca tatagtccaa accaccggct gcttgcatgg ctgctgcagc cgacacaacc
    15241 gccataatca taaacattac atcaatcggt aaaccggcag gttttagccc aaacccaaat
    15301 gaaagaatgg ctaagcccaa gccaccgaac acccctaatc cgataccgcc tacacgggcg
    15361 ccaaccaaaa tgcatagtaa cactatggca aattggataa taaacatcgc agacataatt
    15421 ttttgctcct aaataagtaa atgaaactct gtgtttgaaa aaatctgcat ttttcatcaa
    15481 attaatcggc acgtactata tcaataaata aaactaatct ctataatcta aatcaataaa
    15541 atttctaatt aattaaggaa taatatgcaa ttttcaaaaa tgcacggctt aggcaatgat
    15601 tttatggtgg ttgatggtgt aacccaaaac gtttacctta ccgaagagat gatccgtacg
    15661 ctttctgatc gccatcgtgg tatcggtttc gaccaacttt tattggttga accgccttac
    15721 gaccctgaat tagattttca ctatcgcatt tttaatgcag acggcagtga agtgtcgcaa
    15781 tgtggcaatg gggcgagatg ttttgcccgt tttgttacgc tcaaaggctt aaccaacaaa
    15841 aaagacattc acgttagcac cgcaaaaggc aaaatggtgc taagtttgca agaagacggc
    15901 aaagtgcgtg tgaatatggg cgaaccgatt tgggaacctt cgcaagtgcc ttttacagca
    15961 aataaatttg agaaaaacta tattttacgc accgagctac aaaccgtgtt atgtggcgtg
    16021 gtttcaatgg ggaatccgca ctgcgtactg caagttgatg atgtgaaaac cgccccggta
    16081 aatgaactcg ggccgttact agaaagccac gagcgttttc ctgaacgggc gaatatcagc
    16141 tttatgcaag tagtgaatcg caatcacgtt aaattacggg tgtatgagcg tggagcaggc
    16201 gaaacccaag cctgtggtag cggtgcgtgc ggcacagtgg cggtcggcat tatgcaaggc
    16261 ttgttagata acaaagtgca ggtggattta cccggtggaa cgctgattat tgaatggcaa
    16321 ggagtagggc atcctctgta tatgaccggc gatgccaccc acatttatga tggatttatt
    16381 aaactgtaag ttttcccata gggaggcaag cggtttattt ttaccgtttt tttgcaaaat
    16441 agggttggtt tatacaaaag taccagcgtg gacgctggca ctatcagact gatgtataac
    16501 ttttgtttaa attcaactta tagatggtgc aagcgtcctc gcttgtacca aaacaggttt
    16561 cactcgagta acttcttcac caactccaac accacaggta attcggtaac accttctcta
    16621 tccaaaaaat gcccgccttg atttaaccga atataatccg cgtgcaaatg ttgggctaac
    16681 gcatcactgt attggtgatt cacgacctta tcatctaacg cacttaccac atagcttgga
    16741 aagctcgata cgctcggcaa aatcgcataa tagttggtaa aggcagacag ttcgggtaag
    16801 ttttctaccg gttgatagaa ccccgacaca aacaccgccc ctttaatctt ggcgtaattt
    16861 ttggctaaaa aattcaaaat ggcaatgcaa cctaaactat gcccgacaaa aaccgtttga
    16921 tcggtcagct ccacattttg tgcaagatga tttaaccaac catctaatga cggctttgcc
    16981 gaatccggca tattcaaccg ctcacaactg acaccacact tcgctaattc ttgctccagc
    17041 caaggaaacc aatgcttatc ggcattcgcc gtataaccgt gtacgatata aacctgtgcc
    17101 atttcacacc tcctgattca ccaaagattg taccatagct taatcaggaa aaaacaagcg
    17161 gtgatttttt taaatttttt tgcaactcag gtaaaatagc acgcatttac gcaaagaggt
    17221 catcaatgaa cgtttggatt ttacttgcga tttccatctg cctcgaaatc gctgccacca
    17281 atttactcaa attaagcaac ggcttcacca aagctatacc gactattggc tcgctcgcct
    17341 tatacggttt atccttttac tttctctcta ttattttccg caccctaccg gtcggaatcg
    17401 tgtatgctat ttggtccggc gtagggattg tccttaccgc ccttgttgct tattttgctt
    17461 tcggacagaa aattgacttg gcaggcttaa tcggcatcag cctcattatt gccggtgtat
    17521 tggtgattaa tttgttttct aaagtgtgat acaagcggtt gattttacaa aaaaatctgc
    17581 aaaatcaacc gcttgtaata gttggcaaga attataaccc ctttaaccgc cggtaaagct
    17641 cagtaaattt ttggcgtttt tgttgatatt ctgcatagcg ggcaggggtt ggttggtaaa
    17701 cctgttccag gggtaacggc tgacagaacg tagcaatatc ctgatttggg tttagcgcaa
    17761 tttgagcgag ttttgccgcc cctaatgccg gaccgacatc accactggtg cggtattcaa
    17821 aggttttacc gctaatatcg gcaagtagtt gtcgccagta cacacttttt gctccaccgc
    17881 caattaatgc aatattgtca gcaactacac cagtttcgtg gagtacctca atcccttcag
    17941 ccaaagcgaa acttacccct tcaatcacgg cttttgccat cgttatttgg ttgtcattat
    18001 ggcttaatcc ccaaaatacg cctttcgcat aagggtcatt atgcggtgta cgttcacccg
    18061 ataaataagg taagaaaata gcatcagatg ccgtttggct tgcttcaacc tgttggaata
    18121 aagtcgatac atcttttagt cccaatgatt ttactgccca atctaccgca gaagcggcac
    18181 ttaatattac tgacatcaga tgccaacgat caggtaaagc gtggcaaaaa ctatgcactg
    18241 ctttttgcgg attagcctga aatttatcgc tcactacaaa ataaacccct gaagtcccca
    18301 atgagagcat agcttgcccg gtttggtata agccaatgcc gattgcaccg gcagcattat
    18361 cgccaccgcc tgccacaatc ggcacagatt tcattttcca ttgctcggca agggttggac
    18421 gtaaataacc tgtgatttga ttgccttcaa acagtttcgg catattttca atgtctaatc
    18481 cgcaagcatt tagtagcgat ttgttccaat tccgtttacc cacatcaagc cacattgtgc
    18541 cggaggcatc agacatatca gaggcatatt cgccgctcat caacaagcgg agataatctt
    18601 tcggtagcaa gactttgctg actttttcgg caatatcggg ctggtgttta tcaacccaac
    18661 gcaatttcgg agcggtaaag cccggcatca tcaggttgcc tgtaatttca cggctatttg
    18721 gcacaagctg ttctaactct tcgcattcca taaaactacg tccgtcattc cataaaatcg
    18781 caggggaaag tacattatct gcgttatcta gtaaagtcgc tccgtgcatt tgcccggtta
    18841 aaccaatcgc ttttactgct tgtaaatctt gagtagcgga aagttctaac atcgcttcat
    18901 tggtggcatt ccaccaatct tgcggatttt gttcagacca caatgggtgt ggacgaaaaa
    18961 taggaagtga tttttgtgtg atggcaataa tgtgttgatt ttcatcgagt aacacgactt
    19021 tcacacccga tgtgccgaga tcgattccaa tgtacataaa tattcctttg ttattgaata
    19081 caagcggtca ttttattcgg aacatttgca attttaagtt ttggagatct atcacccctc
    19141 tttagcaaag agggggatca gatcgagctt aactttgcaa aaaattgata aaaaacaacc
    19201 gcttgtagat taacggtaaa tatagctatt taccaaattt tccaaatatt cttgctgacc
    19261 ggaaacaggt tttggatcta acccttgggt ttccactatt tttgccaaat cttcaaggct
    19321 tgctttaccg tttagaattt gtaagcccaa atcttggttc cagccagcgt agcggttatc
    19381 tactactttc tgtaatgctt gatcttccag catttttgcc gcacgtttta gcgaaagggc
    19441 taacacatca attgccccga tatgtgcgtg gaataaatca tacggatcgg tactttggcg
    19501 gcggattttc gcatcaaagt tgaaaccgcc cgttgtgaaa ccacctgctt ttaagatttc
    19561 atacatcaca agggtgtttt cttccacgct gtttgggaat tggtcggtat cccaacctaa
    19621 ttgcggatca cctcggttgg catcaatcga gccaaaaata tctaatgcgg tggcagtcgc
    19681 aatttcgtgt tggaaagtat gccctgcaag agtggcgtgg tttgcttcaa tgttgacttt
    19741 gatctctttt tctagaccaa actgttttaa gaaaccgtaa acggtggcaa catcgtagtc
    19801 atattgatgt ttggttggtt cttgtggttt tggctcaatt aataaagtgc cgttaaaacc
    19861 gattttgtgt ttatgttcca ccactaactg cataaagcga ccgatttgtt cacgctcacg
    19921 ttttaagtca gtgttgagta gggtttcata accttcacgt ccgccccata atacataatt
    19981 ttcgccgcct aagcgtttgg ttgcgttcat tgcggtaaag acttgtgctg ccgcccaagc
    20041 aaagacttcc ggattcggat tggttgaagc acccgacata tagcgaggat tggtaaagca
    20101 gttcgccgta ccccataata atttcacgcc tgtttcagct tgtttttgct ctaaaatatc
    20161 cacaatagtg ttgaaattgt gctgatattc tttgaaattg ttgccttccg gtgcaacatc
    20221 cacatcgtgg aagcagtagt aaggaatgcc taatttttgg aaaaattcaa aggcaatttc
    20281 cgctttttgc ttcgcacctt gcagtaaatc ccccgttttt tgccagcttc tgtcaagcga
    20341 acccacgcca aacatatcgt tacccgtcca gcagaaggtg tgccaatagc aaaccgctaa
    20401 ccgcagatgt tctgccatcg ttttgcctaa aaccacttca tccgggttgt aatgtttgaa
    20461 tgcaaacggg ttggtggaat tagccccttc atattgtact tttgagattt tgtcgaaata
    20521 gttagacata ataatgctcc tatgaattgg aaaaataatc tttcggtaac atcatcggaa
    20581 aattatgcaa aaccttcaat tacgatattt cgtttttgga tttcgattat tggttaatgt
    20641 ggcgtagatc acaaaagtga aaaatcataa tgatattaat aaatatgata attgaacttc
    20701 atttgaaata agcccagact gcttacgggc gaaaaagatg ctcacatttt cataaccaat
    20761 gaggtgaact atgaaactta agtctaaatt attagccgtt gctacggcga ctttaatggt
    20821 actcagcaat gcttctgttg ctaaagatct aaaaatcggt atgtcaattg atgatttacg
    20881 cttagagcgt tggcaaaaag accgagatat ttttgtgaat aaagcggaaa gcttaggagc
    20941 aaaagtcttc gttcaatcgg caaatggcga tgcaaccgca caaatctcac aaattgagaa
    21001 tatgttgaat aaagatattg atgtattggt gattattccg tttaacggcg aagtgttatc
    21061 caatgtgatt gccgaggcga agaaagaagg tgttaaggta cttgcttacg accgcttaat
    21121 caataatgcc gatgtggatt tttatgtttc atttgataat gaaaaagtag gtgaattgca
    21181 ggctaaaagc attattgggc aaaagccgga ggggaattat ttcctaatgg gtggttcacc
    21241 tgtagataat aacgcgaagc tgttccgtaa aggtcaaatg aaggtgctac aaccgttaat
    21301 tgacagtgga aaaattaaag tggtaggcga tcaatgggta gattcttggc ttgctgaaaa
    21361 agcattacag attatggaaa atgcactaac tgctaataaa aataatattg atgcagttgt
    21421 agcttcaaac gatgcaaccg caggcggtgc aattcaagcg ttaagtgccc aagggttatc
    21481 cagcaaggtt gcaatttccg gacaagatgc agatttagcc gcaattaaac gtattattgc
    21541 cggtacgcaa acgatgacag tgtataaacc aattaccaat ttagccgaca aagctgccga
    21601 aattgctgtt gcattaggta aagatgagaa agtagaaatg aatgctcaat taaataacgg
    21661 tatgaaagaa gtaccggcat atttattaga gccggttgtt gtaactaaag aaaatattga
    21721 tgacacagta attaaagacg gcttccatac caaagatgca gtatataaat aaatcacttt
    21781 aatttttcta gatggcgagg cttcctcgcc atttttgaaa ataaggggct aactaagtat
    21841 ggcgaaatta ttagaaatga agaacatcac taaaaaattc ggtgaggttg tggcattaaa
    21901 taatatttca atttcccttg aaaccggcga aatactttct ttatgtggtg aaaatggttc
    21961 aggcaaatca actctgatga aagtattatg tggaatttat cctgccggtg attatgaagg
    22021 ggaaatctat ttttccggcg aaaaattgat tgccagaaat atcaaagata ctgaagaaaa
    22081 aggcatttcg attattcacc aagaattgac cttagtaaaa aatatgtcga ttttggagaa
    22141 tatgttttta gggaatgaaa ttaccaaatc agggattaca gatgacaatg aaatgtatct
    22201 gcgttgtaaa accttacttg aacaagtgca gttggatatt gacccaaaca ccaaagttgg
    22261 agagctaggt ttagggcaac aacagttggt tgaaattgcc aaagcattga ataaacaggt
    22321 acggttgttg attttagacg agcctaccgc ctcgttaacc gaaaaagaaa ccgagattct
    22381 gctgagttta attaaagatt tacaagctca caatattgct tgtgtttata tttcacacaa
    22441 attgaatgaa gtgaaagcca tttccgacaa aatctgtgtc attcgagacg gcgaacatat
    22501 cggtactcgc agtgcagagg gaatgagtga agacgatatt attacgatga tggttggacg
    22561 tgagattacc tcgctctacc cacacgagcc acacaatatt ggtgaagaag tccttcgagt
    22621 tgaaaattta accgcttggc acccaaccaa tacacatatc aaacgggttg ataatgctaa
    22681 ttttgtacta cgccaaggtg aaattttagg tattgctggt ttggtcggtt cagggcgaac
    22741 cgagatggca caatgtattt ttggctctta ccaaggcaaa tatcaaggtg atatttttct
    22801 gcataagcaa ccgattaaaa tcaaaaattg tgcacaagcg attgaaaacc atattgtgat
    22861 ggtgccggaa gatcgcaaaa aacacggcat cattccaatt atgggggtgg gtaaaaacat
    22921 aactctctct tcattaccgc aattttgttt tggcaaaaaa gtaataaatg aaccgctgga
    22981 agaaaccatt atcaaccaat ctattgcaaa actgaaagtg aaaacttcct cgcctgaatt
    23041 agcaattgga cggttaagtg gcggtaatca gcaaaaagca attttggcaa aatgcctatt
    23101 gcttaaccct aaaatcctga ttttagatga gccgacccgt ggtatcgacg tgggagcaaa
    23161 atatgaaatt tataagctca tcaaccaatt agctaaagaa ggtatggcaa ttatcgtcat
    23221 ttcctccgaa ttacctgaag ttttaggcat tagtgatcgt gtattggtta tgcaccaagg
    23281 caaaattaaa gcagatttga ttaaccaaga ccttacccaa gaaaaagtaa tggaagcggc
    23341 actcaaggag taacctatga ataagttaaa atcgattaat ttacaagttt atattatgct
    23401 gattgcaatt gttgtgatta tgagcttttt ctctattgca actgacggtg cttatttaag
    23461 tgccagaaat atctccaacc tgttacgcca aacctccatt accggtattc ttgccatcgg
    23521 tatggtattt gtgattattt ccgcagaaat tgatttatcg gttggctcgc taatgggctt
    23581 actcggtgga tttgccgcca tcagtaatgt atggtggggc tggccgctct cgttaactat
    23641 tatagttact ttaattttag gcgtcatgct gggtgcttgg agcggttggt gggttgctta
    23701 tcaaaaagtg ccgtccttta ttgtaactct tgccggtatg ctggcgtttc gtggtgtatt
    23761 ggtcggctta accaacggga caaccgtttc accgattagc aatgaaatga caaccattgg
    23821 tcaaggctat ttgcctgata tggtgggtat gattttaggt actatcggga tattttgctt
    23881 tattgtttgg ggtagctacc aacgcaaagc cagacaaaaa ctcaatctta ctgtgccttc
    23941 tatgtcgaaa gacacgctga aatacggact agttgcaatt atcgtattag gtgcgatcta
    24001 cctactcaat gattaccgtg gtgtgccatt cccggtattg gtgttggtga ttctagccat
    24061 tgccggcaca ttcttggcca gaaaaaccgc cttcgggcgt cacatttatg cgattggtgg
    24121 caacattgat gccgctcgcc tctcggggat ttcagttgaa aaagtcaaac ttgcgatttt
    24181 tgccttaaac ggcttattag ttgccattgc aggcttgatt ttaagctcac gtcttggagc
    24241 cggcgcacct tccgccgggc aaaatgccga attagatgcg attgccgcct gcgtgattgg
    24301 tggagccagt ttagccggcg gtatcggtag catttatggc gtagttattg gagcatttat
    24361 tattgctttg ctggataacg gtatgagtat gcttgatgtg ccaaccttct ggcaatatat
    24421 cgttaaaggt ggaattctac tgcttgcagt ttgggtagat accatcagca agaaaaaagc
    24481 ctaattggat aacaagcggt caatctcgac tatccaacgg gaaatccggc ataatcgggc
    24541 taaaaatggg aactattgtg cgttttgccg tctttttagc atttgctacg gcaaccagct
    24601 ccaccaagtg cggtggcatt tcgccttgtt tggcaaggtt gcaaaggtag gttatcgctt
    24661 taatccggct cattgagcct tccagttcca acacatacgc caccagtgcc attcgtgcat
    24721 cagcaatttc tcgctgtttg gtggtcaggt ttcccacatc cacctccgca cgcactgccg
    24781 gtaacttttt cggcttggct tccaccaccg ccgacataaa gcgggagcgg atttcggttt
    24841 ggatttcttg agggaggttt gagagtgcaa attctaaaat ttctccccca actccaacct
    24901 ttttatgagt agtccatcct tgtttctttg cctgatacaa aactccttgc tttgttttag
    24961 gtaaacaggc taaacttaac tcagccaatt cttttgccga atagtgtgtt tttagactgt
    25021 tttcactcat aaaaacctct ttaagctatc tgtcggcaaa ttttttatgg tttcgttgag
    25081 cataacgttc aggccaaatt tcttcgggtg atataccgat tgcttcggca ataatacgct
    25141 caccctttaa ccacggacga tctacagcgt ttttcaaggt gctggattga ctgtagccat
    25201 gcttcaatga gagttgtcta agcgaccaac ccgctttatg taaagcagcg atgatgtcgg
    25261 ccctatgcca gtctttcgct gtttttttaa tatcttccaa tgtactcatt agataactcc
    25321 tttcgcttaa cgatttagat aatataccta tttttactaa aaataaagtg taaaaaggtt
    25381 gctttatagt tgatttatga gcaaataaac ttagtttgct gtaagttatt gatttattta
    25441 taggttttta gagtgtaaaa ggctaaaaat ctcaacttat ttttttacag ttatgaaaat
    25501 agagtgtaaa aaggttattg gtttcggtaa ccgaatagaa cagagcagaa aaaatgcaaa
    25561 attaagtaga tctgaggttt gtgatgccct aggaggtatt gcaatatcta cattacaagc
    25621 gtgggaggct gatacaagag aacctcctat atcaaaactt atggctttgt cacaaatact
    25681 taacgttgag ccttgctacc tattaacagg tgagcgacac tcgcaaagcc ttaatgtcac
    25741 aagagagcca cagcctgtgt atgccacaaa accgcagaca gaagaagggg aagtagttgt
    25801 tatcaaagct aagttacaag aggcaataga aacgttggaa gaggtgctgg aaattaccca
    25861 acgggaaatg aagcctgccg gaaaagccca aatggtgtgg gctttctacg agcttctcac
    25921 gcaagaaaaa agcgaaaaag aaaaaatggt gggattactc aaactggtgg cataacaggg
    25981 gacaatatgg acaaacaagc cattctcaaa ttattcagag acaatagcac tgccggcaac
    26041 agccagccaa gccaagagcc tatacaggtg tttaataaca gcaacgtcat cattcaaaac
    26101 ggcgataacg ccacggtgat tgccggctgc acggtggata ataacaccaa cctaacctgt
    26161 gccgataaac aacgtgtaca cgacctcatt cacagcttag agcaacgcaa ccgagccagc
    26221 cgagatgagg gcgcttaaaa ttgttttaaa atccgtttaa acctgctcag acctatgcaa
    26281 aaaataacgt aaaaatagcc catttttttc tatttttgct cattttcgtt ttttgcataa
    26341 ctcaccccaa acaaaaacgg cgaagccttg tatttcctag cctcgccgtt atttttccgc
    26401 ccaaattttt ttctttctct ctatgcaaaa aagatcagta ccccacacta tgttgccaga
    26461 catactcaac aattttaccg tcaacgtcag attaccaaaa atgaccgctt gtaatttaga
    26521 ggagtgttta gagcggaaat aacatcggaa tcacaaacac cgacaccagc atcaccagta
    26581 cagtaaacgg cacaccaact ttaacgaaat caataaattt atagcctgcc ggtgctaata
    26641 ccatcgtatt taccggtgaa gaaaccggcg tcataaaagc ggcggaggct gaaacagcga
    26701 ccgtcatcac aaaaggtgcc ggtgagtagc caagttggtt agcaatcgca atggctatcg
    26761 gcataaccaa aattgcagtc gctgtgttgg aaataaaagc actgataatc gctgttacta
    26821 caaataatac tattagaaca aaatgtacat ctaagccttg cattgcatca agtagaagcc
    26881 taacggttaa atccacacca ccggtttttt gtaaggcaat agaaaacggc atcatcccca
    26941 caatcaacac aatgcttggc atatgaatcg aatcataagc acttttcata tccacacagc
    27001 ggaacttgcc taacattaag caggcaatca acgctgccat cacgtttggc acaatgccgg
    27061 taatcatcag caacaccata gtcagcactg agaacagagc gtgaggggct tggcttaacg
    27121 ccggaggggc gtttttactt tctgccggat ctctaagcaa gaagaaatct cgatgatcgt
    27181 cgtccatacg gtgaatttgt tgccatacgc ccatcaccaa taaaatatcg ccgaatttga
    27241 gtggcgtatc gagcagttcc tcttgtatga tttcatcacc acgttttaag ccgacaatgc
    27301 ttaaatggta tcttgagcgg aatttgagtt cccgaatagt tttaccgata cattctgctt
    27361 caggcataac cgaaatttcc gccataccca ccgagcgata ttgtgaggaa aagtattcgc
    27421 cttttagctc aatcgggcga agtttgagct gttcacagcg ttgttggtaa gtttcatcat
    27481 cgagggcagt atcaagcagt aaaatatctt tcggctggaa aatttcgttt accgaggcac
    27541 tgacggtaat ttttctaaac cgcttagtgc gttgaattgc aatgatgttt agcccttgct
    27601 caatccgtaa attcagttgc ccgatagatt tgccgacaca gtctgaatct gcttccacca
    27661 gcaccatttt ggctcgctct ttaagttgat actcttcaat taactgcgac attgaaaatt
    27721 tttgagcctg actgtgagta ttgctttgct caccattatc caaccaacgg cgggcaatca
    27781 gcatatagcc gatgcccatc actaaaataa ttaatccaat cggagtaaaa ctgaagaaat
    27841 tgaagccttc aaaacctgat tttactaatt cggaatgagc aacaaggttg ggtggtgtgg
    27901 caattaaggt catcataccg ctgataagtc cggcaacact taacggcatc attaaccggc
    27961 gaggagaaat tcccattccc gagcaaatgg caagcaccac cggaatgaaa atcgccacaa
    28021 tgccggtgga actcataaaa gatccaagcc cggcaataca gagcattagc agcaccagca
    28081 ctttggtttc gctcgctccc gacacccgca tcaaccaatc gctgacttgg ttcgccacgc
    28141 cggttcgcac caaaccttct ccgacaataa agagcaacgc aatcagcacc acgtttgggt
    28201 cacttaagcc tgccaacact tctttgactg ataaaatatt agtcaggcta aaaaacagca
    28261 ttacaattaa ggcaaccacg tccatacgca acttgttttg gataaattgc caaatcgcaa
    28321 ataccaataa cgccattacc caaaacagtt cttgcttaag aaaatccggc acaaaagaga
    28381 gcaacgattg cataaccata tcctccaaaa tagttaggga tagattacac cgctttaagc
    28441 aaagaaaaaa gccgataaga tcaaaaagaa aggaaaaagc aatcacgtac ggcgtgaatg
    28501 ctcagtacaa gcggttagtt ttgacgaaat ttttacaatg ggctttccac attcagttgt
    28561 tcgtcaattt cagttaccgc caaaatcagg cttgaacgta ccattttttc caaccgcatt
    28621 tgctgcattt gaaagagcat cgaatcttta ttcgctgcac tcgctttcat attcagcaat
    28681 tcattgatag gcaaaaaatc aatatggtgc aaatctttgg caaatttcac taccgccggg
    28741 tgagaaaatg cgaactcgtc ttcttcctca ttcaaatgct gtttgacttc aaggaaaagc
    28801 gagatatctt caaacacttt aggtgagatc acccccaacc caagcaacac ttttaaacgt
    28861 acttcaatgt cagccagcgg tccttgatgt tcaaacaagg aatccaccac ggattttaac
    28921 gcaaagtccg ttttgcgaaa cactcgttca atcaactgtt caaccgcttt ggcaaagcgt
    28981 tgattggcaa tgctaaggaa accacgcagg gtggtttcct cactgagttt gtcgatgtaa
    29041 tccatcatag tgtcaagaat tattgatata attccaccac ttgagcaatg accgcttggt
    29101 cggttaaacc ggtgtatttt tccaccgctg ccgccacacc gtgatttgca ataaattcgg
    29161 caagttcgac cgcttgtggg tcttcctcat tgcgatattg caatgccatc accacgcctt
    29221 tgaccaagtt ttgatacggt aagccatatt ccaacgtgcc acgcaacggt ttgatcaagc
    29281 ggtcgttttc gctcagtttg cggattggct cacgccctac acggtttacg tcatcgttta
    29341 agtacgggtt ggcaaaacgt ttgaggattt tttcgatata cgctgaatgg gcttgtgggt
    29401 cgaagccgta acgtttaatc agcacagcac cgctctcttc cattgtggct ttcacctgag
    29461 ttttgatttc ctcaatcgca atcgcctctt taatccattt cacgcccgct tgtttgccca
    29521 agtaggcaca gattaaatgc ccggtgttca gggtgaacag tttgcgttcc acaaacgcca
    29581 tcaggttatc ggttaattcc attcctttga tgtttggaat gtcacctttg aactgggttt
    29641 gatccacaat ccactcgctg aactcttcca ccgtgacttc caacggatca gcagggttcg
    29701 gctcggcagg tggtacaatt ctgtccaccg cactatcaac aaaaccaacc actttttcaa
    29761 tttctgcttg ctgctcggca gttaaatgct caaaaatttt acctttaaag aaagttgtgc
    29821 cacgcaccat attttcacag gcgataatgt tcagcggttg ggtgttgcca ctttccacac
    29881 gagccactaa cgctttggcg aacagtgggg caataaagcc aagcacattc gggccgactg
    29941 cggtggtaat taaatcggtg tttttcactt gctcaatcac ggcattttcg tctttcgagt
    30001 taattgccgc aatgtttttg acaatttcca cacggctgtc atcgcccacg attttcacgc
    30061 cgtattgctt gttttggttg atttggtcga tttgagtttg gttaatatcg gcaaaagtca
    30121 cgaaaatgcg agcatcagca agcaatttgc cgataaagcc acgcccgata ttaccggcac
    30181 caaaatggag tgcgttcatt gatgattgtt cctctataaa acgtaggggc gggtcctgtg
    30241 cccgccctaa attttgtagg ataatgggcg ggcacaggac ccgcccctac acaacagggt
    30301 gattatttag ataataatgc taacactttt tccacgtcat ttgttgaggt cagtgttttc
    30361 attgcttgtt catcgtccag tgcgttggta attgcactta aaatttggat atggtcgttg
    30421 ccttttgctg caataccgac caccagtttc gccacgccgt cttcttcatc ggtgaagcga
    30481 acgccgttcg ggtattggca gaccacgata ccggttttca gtacgctgtc tttggcatca
    30541 gatgtaccgt gtggcacagc taagccttcg cctaagtaag ttgaaaccat ttgctcacgg
    30601 gcgaacattg catctacata gcttggttga acaaagcccg ctttaactaa ctgctcaccg
    30661 gcaaagcgga tcgcttcttc tttactgtta gcttgtaagc ctaaaaagac ttgggctgct
    30721 tgcaatttta acccttcggc ttgaggttct tccaccgggt ttgcaaaatt ttgtgtattt
    30781 tgcaccgctt gttgtggctg gtggttagcc agttttgcca ctaagctgtc atagaacgca
    30841 ctgtctaaga agttagtgag cgataagtgt tgagccgttg ccacacgttg tgttgcacga
    30901 gcggttaagt ctttgtgggt aatcaccagt tgtgcgtctt gcggtaagtc gttgatcgct
    30961 aagttttgta cttcaatgtt taaacctgcc gcttgcactt ttttgcgtaa catactcgca
    31021 cccatcgcac ttgaacccat accggcatca cacgctacat agattttgtt gatgttacca
    31081 tttactacgc ctttgctttc tgctttcatc gcttgagttt gtgcttgtgc atcttccaat
    31141 gaggcttcac ctttttgggt tttgagtaag attgacgcaa tcgcaaaagt cacggcacaa
    31201 gaaagaatca cggaggcaac aactgcaaag tgagcacccg gtgcggtcat tgctaaaatc
    31261 gcaaaaatag aacccgggga agccggtgcg attaaaccgc cacctaataa tgagttggtt
    31321 aaaacgccgg ttgcaccgcc tgcaatcact gcaagaatta agcgtgggct cattaacaca
    31381 tacgggaaat aaatttcgtg aataccgcct aagaagtgaa tgattgacgc actaccagcg
    31441 gtttgttttg ctgtgccacg accaaagaac atataggcaa gcaagatacc aaagcccgga
    31501 cccgggttcg cttcaatcaa gaacagaata gatttgccca cttcttgtgc ttgtgctgta
    31561 cccaatggcg tgaacacacc gtgattgatc gcattgttta agaataggat tttcgccggt
    31621 tcaacgatga tagaggttac tggcagaaga ccagcttcaa ccaacgcccc cacacctgca
    31681 cctaatgccg cagagatttg ggtgacgata cctccaatga aagcaaatga aagcattgcc
    31741 aaaatcatac cgatgatccc gaccgagaag ttgtttacca acatctcaaa accgcttttt
    31801 actttgccgt caatcgcttt atcgaaggtt ttgatcgccc agccgcctaa tggacctaca
    31861 atcatcgcac ctaagaacat cgggatttca gaacccacga taatacccat tgtagtaatc
    31921 gcacctacaa ccgcaccacg ctcaccaccg actaatttac caccgctgta accgattaac
    31981 agtggcagca agtaagtaat catcggaccg accagtttag caaaagtctc gttcggcaac
    32041 caaccggttg gaataaacag ggcggtgata aagccccacg caataaaggc accgatattc
    32101 ggcatcacca tattcgataa gaaacgccca aaaccttgga ctttcacttt taaattagac
    32161 atagaaacct caaaaaagga gagaaaatta gggttgaaaa aatcgtgctt aaattagcac
    32221 cactttcaaa agttacaaaa atgtttctct cagaaatgtg aagtagatca cattaaattt
    32281 ttttgggcag atttgaaaat gtgatctatc tcacattttt ggagtaaaga tggcgtaagc
    32341 gtcctcgctt gtgccaagca taattctatt tttgcgatct acatcgaaaa aataaaatga
    32401 attgctaacc ctaactgttc acctatatta ctatcagcat aaactgcact ttagaggttc
    32461 ataatgagta gaaaatataa gttttatgag aagagcggag cctattttgt tagttttgca
    32521 acagtttatt ggattgatct atttattcga gaagattatt ttcaaaccat tatccaatca
    32581 ttgaattatt gtcgaaaaca aaaagggatg attttatatg gctactgcat tatgccaagc
    32641 catattcatt tattatttca agcaaaagac caaaacccgt cagatttaat aagagatttt
    32701 aaaacattta catctaaatg ttgattcagc aaattaaaac aaatccccaa gaaagtagaa
    32761 aagaatggtt attatggatg tttactcggg cagctaaaca acgaaataat gtcaagcaat
    32821 accaactttg gcaacatcat aaccaaccga taacaatcta ttctcagaaa ttttttgatg
    32881 aaaagctaaa ccatattcac aacaacccta tcgtaagcgg ttttgtatgc gaggcatttg
    32941 aatggaaata tagcagtgca agaaattatg ctaacaactt acctgtttta ttagatattg
    33001 atatatgtca gtgaacggca caagcgtaaa cgcttgcgcc atcgttacat ctataaatta
    33061 actagagaac tccatcaatc ccgttacttt accctcatca ttcactgcaa ttaatgagtg
    33121 aattttcagc tctttcatta aatcttcggc tttggctaaa aattcggtat cgttaatggt
    33181 ttttggatta cgggtcataa tttcgtcagc ggttttattt aggctctccg ccccgaattt
    33241 cgctaatgtg cggcggatat caccatcggt aataatgcct tccaatttat cgtcttgcat
    33301 aattaccgcc acccccatac gcccttcgtt catcacagat aaacattcgg taaaggtggt
    33361 gtctaagctg gcggttggca ctttacgcac catcacatct cgcactctgt tcaataattt
    33421 acgaccaaga ctgccacccg ggtggtagcg ggcaaaatct tcagggcgga aatcacgggc
    33481 tttcattagc gcaatggcta acgcatcgcc taaagccatc gttaccaaag tagagctggt
    33541 tggagctaga ttattcaggc aggcttcacg ctctacacca atatttaaaa tcacatcggc
    33601 atttcgccca agtgtggagt aaggattacc tgtcatcgca ataattttat tgccgaaatt
    33661 tttcaggctt ggaatcagtt tattcacatc atcagtttca ccactgttag aaatcagaat
    33721 taccatatca atcggtttca gcattcccaa atcgccgtga aaagcctctg tcgggtgcaa
    33781 gaaaaagctc ggtgtaccgg tggaggcaaa agttgccacc atttttttgc caaccagccc
    33841 cgattttcca ataccgccaa caaccactcg cccttgacaa gcgagtatca tatttaccac
    33901 ctcattaaat tcttcagaaa gtcgatggtt tagcgtgata atctgctgag catagaggtt
    33961 taacgtttct tttgcactgt ctaaatgatt catttttacg cccttataca actaacagct
    34021 caatcccttt cgctttaatc tgttctctca tttcttgaga taatccttca tcggtaatga
    34081 tagtatgaat tttttctaac ccacacacaa tattcggact tttacgtcca aacttactgg
    34141 agtcggcaag taagacgatt ttatttgccg catcacacat tagcgaactg actttatata
    34201 cttcattgaa agtcgttaat ccaacgtcta aatctagtcc gtctgtgcca ataaagagta
    34261 tgtcaaaatt tgaaccaata aaagcggatt cagcaaaata accgtgaaat gaagctgatt
    34321 tctcacgaaa tgtaccaccg gaaatcagta agttatagga tttattgagt ttcaaaatct
    34381 cattgaaaat agttaaacta tttgtcatta aagagaggtt atcaaatgct tttaaatggg
    34441 gaatcatttg taacactgtg ctgcctgcat caaaaataat agagtttccc tcattaatta
    34501 actctgcagc tttttgcccg atagcttgct tttgttgaag attaatgccg gttttctgtg
    34561 caagcggttt atcgtctttg tcctctttat tgattttctc ttcccgttca agaatttctt
    34621 catcaattaa gactacactg ccataagtac gcaagacttt cttatcttct tcaagtgaag
    34681 ttaaatcttt acgaatggtt gcaccagtta gcttaaaata gtctgccagc acgtccactt
    34741 cggttctacc gtgctgagct aaataggcta aaatttgctg tcttctttca aaaggtttca
    34801 ttgttcctcc aatttagcaa agattggagc gagctaatct gtgctaatgc tgttttaatt
    34861 tttcaatgac atctagtagc gatgccattc ttttatcggc aattgaaaat ttttcatttt
    34921 gataatcggt attcaccaaa aatgtagtta aattagcggc tttggcggca attaagccgg
    34981 ttacactgtc ctcaataaca atacactctt gtttttcaat gcctaatttt ttcactgcac
    35041 tcaaatagac cgcagggtgt ggcttgccat attcttcgtc cattgcactg catcgaatcg
    35101 ggaaataatc ccacagtttt aagcgatcaa acaccgcttt aatcaccacc ggcggcgatg
    35161 aggttgcaac ggctaacttg atctgattag attttaagaa atccaataat tcatataaac
    35221 ccgttaatgc tttgccgtgt tgttcaatcg cagcacaacc gtgtgccaaa atggcttcac
    35281 ttaatgcttg gttggaaaca gataactgaa attttttaat ccaagtgtcg ctcaactcat
    35341 caatccgctt acctctggtg agttgttcgc aatcttgctc ggtgatatta atacctaagt
    35401 cggctaatac tgcaatttga gcctctgccc attggggttc tgaatcaatc attaccccgt
    35461 ccatatcaaa gatgacacta ttgatctgca tatttattct ccattatctc atttttaact
    35521 aaaaaaatat tcaaaatgaa ataaaaaatc gcattatttt tgatctgagc aactatatcc
    35581 ggaagatatc gccactttta aggcaaattg tgaacgttta tcgcttggaa agattttttc
    35641 cggcacaatt tgatcaagag acaaaccaat cacagacgct aatttctgcg gtctggcaaa
    35701 gacggaaaat cctttcatac aaaggctatc taccactttt ttctcatcat caaacgccaa
    35761 cacaataacc gatttaggat taaaacgacc actattacgc ccaaccgtta ctttgccttt
    35821 cttactgatt tccataaagg cttggttaaa ggctttaatt tgaagccaac ccaaaacggc
    35881 ttgtaacagc cacattatca ttgccaatat aatcagaagt gtaattgaat ccatcattgc
    35941 tccttaataa aaagccacag agcctaaacg tacctgcgat ttcaaaacaa tacgctttcc
    36001 cctgtggcat attaaccggt gtggcgaatt aacaagcggt ttattttagt cattttttgc
    36061 aaattgttgt aaaaaaatcg acaaatttga ccgcttgttt gcttagaaca ttacctgacc
    36121 accggtaata ttaatagact gtcctgtgca ataagctgcc tcttcgccgg catagaattt
    36181 taagacattc aacacatctt gataatcaca gccacgtttt aaaggcactt tatcaatata
    36241 aacttgctct acttcgcttt ccggaatgcc gagttttgcc gcatattgcg gaattaacga
    36301 ttggaacatc ggcgatttca ataaattgcc taacattaat gaatgcacgg taatgccttt
    36361 atctgccaag tctaatgcta acgattgtgt taaaccaaca ccaccaaatt tcgctgcact
    36421 ataaccggaa ttatacttac tccctacttt acccgattta gaattaattt gaatgatacg
    36481 ccctttaata ccgtcacgaa tcattaagcg ggagaagtgt tttgcggtta agaaataacc
    36541 ggttaaattc acttttaccg acaaatcaaa atcgtttaag ttgaaatccc aaataggcga
    36601 tgctttcgct gtgcctgcac tgtaaaccaa tacatcggca cgcccgaata cggtatctgt
    36661 tgctttagct agtgcttcaa cgcttggttc atcagccgca tcgacttgca ccccaatcgc
    36721 atttccttcg ccttgtttgg cattaatgtc ggctgccact ttttgtgcat tttcgccgtt
    36781 taagtcagca accaccacac gataaccggc atctgccaag ccttcagata aaaaagctcc
    36841 aagtgtttga ccgccgccaa caacgatagc aacttttttt tcattgttca taatgtattc
    36901 ctctaaattt tcaatttgaa attattttta atccgtcatt aatcacgctt gataatcaag
    36961 gttgagttct ctgttaagcc ttgcggcggt gttccgataa cgtgaacgct tcccggaaat
    37021 tccgcatcat ttgccccgtc aaaacggaag gtaatgtgtc cgagggcgga taagttttca
    37081 gatgcaacgc taccaactgc cgtaatcgga taagctacgc catcaaattc agcgatatcg
    37141 cctacttcta aagggctact taattcagat ggattatgaa tgaagcagta atcttgtaaa
    37201 tctgccggtg cgccttgttt aaaagtgatt aacatatcgt ctgttaatga ttcctgagca
    37261 aaattcccga ttttggtaaa ggtcgtttga taaataatcg tcatataaat gttctctcgt
    37321 tttgacaagc ggtgcatttt gcaaaaaatt tttcaaattc caccgcttgt atcgtggtta
    37381 attattggta gatgaatgcg gaaactgccc acgcaatcag cacggtcggt gcaccggtta
    37441 aaaaacggct gactaatact gacggtacac ccacccgtac ggtatcttgt tttgcttccg
    37501 ccattgaaag acccacagga ataaaatcac aagcggcttg agcattaata gcaaagagtg
    37561 ccggcagtgc taaatgtggc ggaatattgc ctaaaccgat ttgcgtaccg actaacacgc
    37621 cgataacttg tgcaatcacg gctcccggcc ctaagaatgg ggaaaggaga gggaaggaac
    37681 aaatcaacgc cagtgtaact aaaccgattg ggctgtttgc cagaggggtc aagccgtggg
    37741 caatcacatc acctaaacct gacgcaatga tgatcccgat taacgcagaa acaaatgcca
    37801 taaacggtaa aatggtttta agcaccgtat caatagtgtc tcgtccggct tggaagaata
    37861 acgccgccac cgatcccatt cccataccga tttttgctaa taaaccatca ctttgttcgg
    37921 tgattttttt gctggagtca tactcttttg ccgttgctgt cgttgatgaa gttggttttg
    37981 cttgctctgt tgccacaggc acacttccat cggcatcaat taagcgaata ttgttttctt
    38041 tcaccgctga aacataaata tcttccacaa taaattctgc taaagggccg gatttaccgg
    38101 tggagtgaat gttaatggtt ggaatacggc gttttgggta aagcccacag cgtaataccc
    38161 caccgcaatc aataatcgca atcccgattt cttctgccgg cggttcgcct tctttaaaac
    38221 catcaaccgc ttcccagccg gttaattcca ccaatttatt aacaatcgca gggcgagtgc
    38281 cgccggtcat ataaagcact tttttgcctt ccacaacagg taatgttaat ggaccacccc
    38341 aaccaccgtt gcctttttca atgtaaatcg ctttgttgct cataatctat tcctctgtat
    38401 ttcagttact tggttagtgg gataaatgta cttctctatc taaggtgatc cccattcgtt
    38461 tttcaaagaa tgcggtggtg aagtctgtta tccagccacg gaagaaattg gtaaacatac
    38521 cgactaataa ataactgatg gctaaaggcc caagtggtaa gcctaaggtg gttaaaccgc
    38581 tggcaatacc taaataaacg aataattcac ccgggttgat gtgagggaat aagccgttca
    38641 tagagtggca actataagag gctgcggcgt agtagcttgg tttgtaacgc tccggtaaga
    38701 aacgccctaa gcttaatgtc atcgggttac agaaaacgaa agtgccgatc accggtaaga
    38761 tcaaataacg tgaaataggg tttccggcac tacgttgagc aagtcgttca atccgatctt
    38821 gccctacaaa tttaatcagg gcattcatta caaccaataa tgaaattaag agtggcaaaa
    38881 taccgtttat cataccgact aaggtttcac cgcctttttg gaacaggccg ataaaccatt
    38941 ctgctccgtg tgtaattgct tcaatcatac cgatctccgt ttttcaaaat aaaatatttt
    39001 tctttcatct ctgttatgag atgtgcaaat agtaaattat ttttatttta aaaactgtga
    39061 cagcgatcac aaaatgagaa acaactttca aaataaatta ttttttcatt ttaaatcaaa
    39121 acaaaagtga agtttgaata ttagatgggg gaaaaaagga aggctaaatt aaaaaagctc
    39181 aaacaataat gtttgagctt tggttttatt caatgacttt tgcagtatat ttgaggtttc
    39241 cgaccgcttg tatcagggct tggttatcca cactttcttt taccaccaca gtcgcttttc
    39301 gctctttcat tgaggctttg gtggaaatca caccttctac tgctcgtagt tctttattga
    39361 ctaaataaac gcagagctgg cagtacattt ccggaatatg gaggacgacg gttttctctg
    39421 cttgtttcgt ttcaatttga gctgtttttt cttgtgcgta gcttgaatga accaaaccaa
    39481 aagccaacag catcgttgcg atatattttt tcattttatt ctccgctcca catttccaaa
    39541 atgtaaggca atacataagg gtaagttaaa aagaacatca ccaccacaga aactacccaa
    39601 tacaaaatga ccagggttct gcgggagaag tatttggtgc agatgatttt gttggaaaaa
    39661 ttcagcaacc agaagccata gccaaacgca gcgagcgaaa tcagcaacat cggcagttgt
    39721 agaaactcca gttggtttag ctccatcaac caaggggaag agatgccaaa cactaaataa
    39781 accagtggcc caatacaaca gaatgtggag gtcaccgccg acactatggc ggtcacgcac
    39841 attgtgataa atttatggtt ataactcttt agagatgaat tcatagccaa attctttcgc
    39901 ctcaaacgag tttaacgggt caccacctac ctcagcataa gggtagccaa agttattcag
    39961 tggtgtaagt tccggttgtg gatataaatc gaaatcacgt ccgtgattga agcctaccca
    40021 gttcatttcc cagttgccga ataagtattt cgagaccgct tgaacatcag catcatccac
    40081 gtcttttttc tcggctaaac gcattttagc gacatcggca gaatctacag gcacccagcc
    40141 gaagcctgct aaatagaact ctgcacggca gtgctgcccg ccgctttcat tcgccacgcc
    40201 atcttttgag ctaccaaatg cggttttgga atattgcccc atttttaccg cttgccctaa
    40261 acgaatacca aacacttcac gagcaggaat gttagaggca cgagcaagtg ctacaaacac
    40321 cgagttgatg tcggtacatt taccttttag cactttggtg gtgagaattt tctccacatc
    40381 gccatcgcca cagcctagca ccgagttatc acgttccata ttatcaacaa tccacttgtg
    40441 gataagttcc gcttttttca acggattttg ctcgttaccc acaattttat cggcatattc
    40501 tttcacaata ccgtctgttt taatgtgagc cgttggtttt aaatactcca atacatcaac
    40561 agaataagtg attttctccg gcatttggta atttgccaac gccccttgag ccataggctc
    40621 acgatcttgg gtttggatca ccattttcac ttttagatca cgctttggtg catcagtatc
    40681 ccaagtggca aacaaggttt ttgcaccata agcgttattt tctgtgatat aggcgttttt
    40741 gtaacttccc tcaaactcta tcgattttac cgtttgataa tcggtatcag acggcaacgg
    40801 aatccacaaa ttagttgttc ctttcgagcc ttgtggcaca actaaatcat aagattgcgt
    40861 aaattcgtaa gtgtgggtag tggtgtggaa attgttctgt tgaacaggag cagcaaaagc
    40921 cgtcgcacag cttgaagcca ccaaagaggc aataagttta ttcattgaaa tgatccttat
    40981 tagagtgaaa aaacagttaa aacttaacat attaagtatt ctaactacgg attgaggttg
    41041 catattagcc aatcgagatg ggaaaagcaa tgggggagat gattttttag atagtgcaag
    41101 cgtccacgct tgtactagaa acaaagctaa aataatcaat aggcacaagc gtggacgctt
    41161 gcgccatcag agagaataac aagcggtcaa aaatcctcaa aattttacaa atagattttt
    41221 tgccctaatt ttgcactttc aaatacgctt tcgatcactt ttagcacaaa aatgacgtct
    41281 tcgagtttga taattggctc agcattgtga gcgatggctt ggtacaaatc atcaaacaag
    41341 ttttgataac tgacttcggc atttcgatag ggctggcgaa ccacattacc ttccacttca
    41401 gtgtgtaaaa ttccccagtt ctccttttct tcgatattcc aatctttgcc aataggttgt
    41461 acgccatttt tcagcaaatc ttcttgatta tcgggagttt gtttcagata agagcctaat
    41521 tttccgtgta atacaaatgt cgggcttggt tcacgagcat atcggctggc tttcagcacc
    41581 actttaaaac cgttagcata atagagctga atatcaaagt tatcatcaac caatgcccct
    41641 tcgtgctgat aacgcacatc agcatacacc gcctccggct tcccgaaaag atacaaagct
    41701 tggtcaatta aatgtacgcc taaatcatac actaaacctg taccaagctc gcctgattct
    41761 ttccatactt taacattttt ctctttcgca taacggtcaa aacgaatttc gcaatctacc
    41821 aactctccca gtaaattttg ctccattagc gattttgccg tcgcaatatg gctgtcccaa
    41881 cggcggtttt ggtacacata aagcaccttt ccttgccgct ttgcaagttc cgccaactct
    41941 tccgcctcag ccactgttgc cactaacggt ttttcaacta aaacgtgttt gcctgcaagc
    42001 aatgactctt ttaccattgc ataatgggtt tgattcggtg tggtaatcag cactaaatcc
    42061 acttctggcg ttaaaagagc gttgaaatcc cgcactgttt ctgcattcgg caaccattct
    42121 gccgcattgt tagaggttcg ttcatatacc tttacaatat taaaacgagc atcttgtttc
    42181 aaaaacggga tatgaaaaac tcggctggaa aagccggcac ctgcgatggc aatattgatg
    42241 ggttgcatag acaatcctca taaaattttg atgttatctg cttaaataaa caagcggtca
    42301 aattttagga aaattttgca aaaaacagat aaaatacgac cgcttgttta actaatcata
    42361 ggttttgcaa acaatatctt tttcttcaca aaacatttca ctgtggtttg cacggcattg
    42421 cttttgtaca tttaatttgg ctctgcctcg gctactatgt tcactacgat attcctgagt
    42481 aaatggcttt agagtacaaa tgtgaacctc tttggaatca agtggatctg agacaccaat
    42541 tataatcggc atagggatac cggcttgccc cgtaggagta accggcacga cacagccact
    42601 taatagtatg cttaaactta ttaaccaata acgcatattt ttctcctttt aaaatgtata
    42661 ataaatcaga acggagcatc tttatcgtta cagtaagctc ttaatatctt gcctaatttc
    42721 ttggtatttt tattaaaatt tcggcattta gggcatacca aaagatgcaa ctgcaaacgc
    42781 atatgttgtt gcaaacgaag tttctcatca caagactgtg aaataagttc tgttgcttgc
    42841 ctgcatttca ttttgattcc tctctaaacc aattgcgaga taaacactct tgtaaacgta
    42901 aacgagcacg atgaagtaat acatttaagt tagagagaga aatttcacat tcttggcaaa
    42961 tttccgcact actcatctcc aaatgttcac gcatcataaa tactcgtgct tgctgggcgg
    43021 gtaatttatt taaacaaata tcaaaaattg cccaaaattc tttttggtaa gttgattgag
    43081 ctacccccct ccattcagtt acatcttgat gttccaacca atgatcgtgg cgatcgaaaa
    43141 aggcacttgg tgattcttct tcctcacata gagaactgac tggaatatgt cgttttcgtg
    43201 ttctgagtaa atctaaaatt ttgttcttta aaatagcaaa aatccaagtt ttaagtgcag
    43261 cttctcgccg aaaagaggct gaatgcttat aggcattggt taaggcatcc tgtacaacat
    43321 cctcagctaa atctttatcg gctagttgta gggtggcaaa tttaaccatt tgctttctaa
    43381 tctcttctat ctgtgtagaa gatagactct tcatatatct cctttaggga atacccaata
    43441 aaaaattaca cttattttgt aagaattcta gatcagctga gtccaaaata ataacaacaa
    43501 ttcataaaaa aaggatagtt cgatgaaaaa attaacaatt caagatgtta cacccgaaac
    43561 tattttctac caacgccgta aaattattac cgcgttaggt ttaggtattg cagcttctgc
    43621 attgcctaaa gtaagtatgg cagaaacaca aagacacttg caatcactac agtttaaacc
    43681 ggatccaaat aaagggcttg aactcactcc ggaaaacaaa gtaataggct ataacaattt
    43741 ctatgagttt ggcgtggata aaagtgcacc agcaaaaaat gcggcaaact tcaaaaccga
    43801 tccttggaca ttggaaattg gcggagaagt tgaaaatccg tttagcttaa atcatcaaca
    43861 attgcttaat acgtttccac tagaagaacg catttaccgt tttcgctgtg ttgaagcgtg
    43921 gtcaatggtt gtgccgtgga tcggctttga gttagctcgc ttaattgaga tggcaaaacc
    43981 aaccagtaaa gccaaatatg tggtctttca cactttatat gatcctgcac aaatgccggg
    44041 gcaaaaaaat cgcttgctag gcggtgggat tgattatcct tatgtagaag ctttacgctt
    44101 agatgaagcc ttaaattcat taacactact atccgtcggt ttatacggta aaaccctgcc
    44161 accgcaaaac ggtgctccta ttcgcttagt cgtgccttgg aaatatggct ttaaaagtat
    44221 taagtcgatt gtgaaaatca cctttgctga aaaacgccca ctcactactt gggaaagtct
    44281 tgctccaaat gaatatggct tttatgctaa tgtgaatcca aatgttgatc atccacgttg
    44341 gtcgcaagca tctgaacgag taattggtgc aggcgggtta ttatccgtca aacgccaacc
    44401 aacactaatg tttaatggct acgaaaatga agtggcaaat ttataccgtg gcttagattt
    44461 aaaggtgaac tactaatgct aacctggtta cgtatactca ttcatcttgg ctgtttatta
    44521 ccgctagttt gggtagcaca gctattatat acgggtaatg aaacaatatt aggtgccgat
    44581 ccgattaaag agctggagca ttttctcggt tatggtgcaa tcgtcatttt ttgtataatg
    44641 tttctacttg gtattgccct acaataccta aagaaaaacc agtatcaaat tttacgccgt
    44701 ccattgggct tatgggcgtt tgtatgggca gctctacata tttcaagcta tctattgtta
    44761 gaattaagcc ttgatgtaaa attatttttt agcgagcttg ccggtcgccc atatttgatt
    44821 ttaggagcag ctgctttttt cattctaagc ataatggcaa taacttcgct gcctatgcta
    44881 aaacgaaaat taggaacacg ctgggggacg attcaccagt gggcttatcc tgcattagta
    44941 ttggcgatgg tacattatta ttggtcggta aagagcgtaa cattcgatcc aattatcatc
    45001 ggagtcttag tgctgtttat tgttgctcat aaaatacagg ctaaatttgc aaaaaaatag
    45061 caaaatcaca ccgcttgcag cagtcaaact acatacaaaa aataaggggc tgtcctagat
    45121 aacgactaaa acatccattt aggttaagat aacccaatga gaaaaagtcg tctaagtcag
    45181 tacaaacaac ataaactcat tgaactgttt gtagcaggtg tcactgctcg aacagcggct
    45241 gagttggtga atgtaaacaa aaccaccgct gcctattact ttcaccgttt gcgattgctc
    45301 atctatcaac atagccctca tctggaaatg tttgagggtg aaattgaagc ggacgaaagt
    45361 tatttcggtg gtgctcgcaa aggtaaacga gggtgtggtg ccgcaggcaa aactgccgta
    45421 tttgggctgt taaaacgtga tgacaaggtt tataccgttg tcgtaccaaa tacgcaatct
    45481 gccacacttt tgccgattat tcgggagaaa gtgaagccag acagccttgt ttacgccgat
    45541 ttttatcgaa gctatgatgt gcttgatgtg agtgaattta accattttcg tatcaatcac
    45601 agcacacact tcgctgaaaa tcacaatcac attaacggaa ttgagaattt ttggagtcaa
    45661 gcaaaacgtc atttacgcaa gtttaatggt attccgaaag cccattttga gctatatttg
    45721 aaagaatgcg aatggcgttt taatcacagt gacttaaaat ctcaaatatt ccttttaaaa
    45781 caattggtta aaggggggtt aggttagtta tctaggacag ccccaaaaat aatggcataa
    45841 gtgactcact tatgccattt tttatatgct ttaacgttta ttaaaaatct taaaaatatt
    45901 gttggcttcc tctaaataat gcaagatttt ccgcactcat attgccgtta ccaacacctg
    45961 ctacgacaat accttgatag cctacatcta acaaggcttt agtgggccaa ttttcacaat
    46021 ctgctcacct ttgatattcg ccaatgtttt catttctggc actgcttcaa ttaaggtttc
    46081 gatattcaat tgccctgctt gataagcact actatttgca ttctgctgac taccagcaat
    46141 cgttccgcct gtcgctaaaa tagtgatatt aggtaattct gccgcattag ctgttacaaa
    46201 taagccacct aacattgctg ctaatgctaa ttttttgaat ttcatataag gctccttaat
    46261 taaaaaaact aaagacagat aataccgata aatcaatgta aattatttga tctgatacat
    46321 aaaagcggga attaattact gggtagaaat aggttatatc gctattttca tttgggttat
    46381 ctactcatag aataccttta actaaaacat aataagcaga atttacgcaa aattctccga
    46441 cagacaagta aaaattcaca atttttcgac agagatcgca aaaatacaag cagtttattt
    46501 caattatatt tccattccca aggctcagac acaaaaccac ttaccactgg attttgatga
    46561 atataattta acttttcatc aaagaatttc tgcgaataga tttcaatagg ttggttatga
    46621 tgttgccaga attgatattg ttttacatta caacgtcgtt cagccgcccg agaaaacatc
    46681 cacagcaacc attcccgacg gttttcttgc aaattatctt taattctctg aataagtatt
    46741 ttagcggtga atgttttgaa atctcgtatt aaatcagaag gattttgatt ttctgcttga
    46801 aataataaat ggatatgagt tggcataata caataggcat acaaaatcat tcccttatgt
    46861 tttctgcagt aatctaaggc atcaacaatt acctgaaaat aaatctctct cacaaacaca
    46921 ccaacccaat aaacagttgc aaaactgaca aaataagcac cgctcttttc gtggaattta
    46981 tattttcggc tcatagcctt tctcaactta aaaaggaata agaaaaatac cctattcaaa
    47041 attaaattcc acaagccaat ataacttttt cgatctagat cgcaaaaatc attaaaactt
    47101 gcaaagtgtt gataaaattt aaccgcttgt gataagccaa agaaatactg aaatgatagc
    47161 aatggatgga taaataaaat gatgacgcaa gcgtccacgc ttgtgccaaa aacaaaagcc
    47221 caaataaacc aaattttact tggctgattt aggcttttat cttatgactg aaatactttc
    47281 agaacaggaa ttaactggca caagcgtgga cgcttgcacc atctgggtta aatccttata
    47341 ttataaggtt ttaacctact gtgcagactc aattttagtc actatgccaa ataattagtt
    47401 aaaggaaatt taagctagct tatctaggac aaccccattt agaatttata acttgtgcta
    47461 aacactatag ttctccctct tgcataatta ttgagcgtat aaataggttc atcactgata
    47521 tcagaattgt aaaccctctg acttgctgca tcattattag catcaagagg atcaatgtac
    47581 tttttgtcaa agacatttta tagttctaac tttaatataa gcgacttgat tggttcgtag
    47641 ctcacgtaaa aatcaacaat aataggttgt ttatttacgg tttcttcaag ccgatgagtt
    47701 tctttcacaa tatgtgttcc aggataataa gtatagttaa actcatctat cgcaatacgt
    47761 ttactttttc cgtaatattt tgccactccg ccaatttgta gtctttgatc aaaccatctt
    47821 gtccctaact ccaatgagcc atagtctctt ggtaacgctg aaattttgct ggcaccaaaa
    47881 ccttgagttt taaaaccata atgagaagcg gcatctaccc ttgaacttgc atctgtaaag
    47941 gatacgggtt gattcgtatt ttgacgagca taagctaagt tgccatagaa agagcctata
    48001 tcataactaa attctaattc tatccctttg atatttactg gtttttcata gtttttgtgg
    48061 aaaatgaata aactaggagc actaccaaca gcggtatgtt gtacattata aatatagttt
    48121 tttaagtgag tgttataaaa tagcacttta aatcctaatt tatcttcttc actaaatacc
    48181 cgttctttga atccattaat accaaactgt gttgttttcg cttgctctgg ttgtagtgcg
    48241 gtatttacac catagtcacc taaattagag aagaatattt ctttaatatt aggggctcga
    48301 tgtgtttgag aataactaat aaagggcgtg aataaatcgt gaatataagc agacaaggtt
    48361 gctgaataat taattcgttt atgttttcct gttgctgtat gaggaaaatt gacatcagga
    48421 cagttaaact ctcccaattc aggatcatta ggaaaatcct catctggaat atatgtacaa
    48481 tgtttttttg caagttgtct aattgagatt gtaatgacct taaatgctca aactgcctat
    48541 ttctacttgc ctgtctaata tctgactcca attctctaat cgcaaaagga aaatatttaa
    48601 aacgctgacc aataaaccga gcggtattaa tatttgcgtt tatatccaat ccccatatac
    48661 catagttaaa gtgattgtcg agataaaaag tatcgaactt ctgttcgcca tcaggctgga
    48721 aagttgaagt ataaagcgat ttacgaacgg ctgtcattcc tacagaatcg ttgttttcgc
    48781 tggtttcgcc attctctaag ttatagttta attccgcagg atgtctattt ttactatagc
    48841 ggttctttaa acgattaaaa cctaatattg cttcccattc tccatcttta aacggagaca
    48901 ctttaaaggt gttactcaaa tttaaggttg tcgctttgtt gttaattgcc aaaccttgaa
    48961 ttaaatattt attattaatt cagctccctt tgttgtagtt ttgtttgcct tgattttccg
    49021 cataggccac ttgaacatct aaccaaggtg actcgggcaa gtgaaaacga taatttaact
    49081 ggttgttttg gttttttaaa tgtctacccg ctacctgact ttggtaatag tgtgatggtt
    49141 tggtatttat cgccatattc caatttagcc atacgactca ttggtttttg ttttaaatga
    49201 gctgcattga acggtgaggt ggtagaactt tctttatcta gatcatcaat tttactaagg
    49261 tcaatcgagc tatccgtaac ttttcgtccg cctcctattt tataattttg agagattttg
    49321 cgccaactat gcccaaataa tgctcctatc caagtagagt cattgaattg gtatttgcca
    49381 gcgagcattc ccatataatt tgggcctact gcattattag ctcgcccaaa tccagaacct
    49441 cctcgaatat taacagaaac cacaccttga gatttatcca tttgtgtaaa tgcgccaggc
    49501 atagaacgaa caatagtctc taagctttgg tttgattcca agattttatc tcgggtgctt
    49561 attgcccctg ttttaatgaa aacttcatct gtttttttat tgcttgggct ttaacctcaa
    49621 ccgtatctaa catataacct tcctctgcga ataccctagg taaggggcag ataagtgttg
    49681 tataagtcag gtgaaataca tattttttga tcttcatttc tattgtcttt cctataaaaa
    49741 atgtaattaa atttaaatcg caacgctaaa gacactgaag aaagaagt
//
LOCUS       DANLZC010000003        49302 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.003, whole genome shotgun sequence.
ACCESSION   DANLZC010000003 DANLZC010000000
VERSION     DANLZC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 49302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49302
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.003"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..220)
                     /locus_tag="PXE44_000129"
     CDS             complement(<1..220)
                     /locus_tag="PXE44_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5587684.1"
                     /translation="MPKSWELSLTARSNVIEILQSVEDYTGHWLSAVSLWEQLQNKLD
                     LIAFMPTIGRRREDGTLETFCRNYRIVYE"
     gene            complement(213..404)
                     /locus_tag="PXE44_000130"
     CDS             complement(213..404)
                     /locus_tag="PXE44_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587685.1"
                     /translation="MLFKEQGYDEFLAEQIKAGQEAIARGETFTKEQINNELNHLFAD
                     MQKRQQLRENENLFEVNYA"
     gene            complement(694..1284)
                     /gene="yraP"
                     /locus_tag="PXE44_000131"
     CDS             complement(694..1284)
                     /gene="yraP"
                     /locus_tag="PXE44_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDL5587686.1"
                     /translation="MNKLANKLLKGSLLVLGFISLQGCITTAVVTSAAVATKVATDPR
                     TTGTQVDDEILEEKVAYNINKDEQIKQEARINVVAYNGKVLLIGQAPSMDVVENAKNL
                     AAGAEGVTEIYNEIRQGEKIGFGQITQDSWITTQVKSKLLVNSEVKATEVKVVTENGE
                     VFLMGKVSQNQADAAAEAARNVGGVTKVVKVFSYAQ"
     gene            complement(1294..1878)
                     /locus_tag="PXE44_000132"
     CDS             complement(1294..1878)
                     /locus_tag="PXE44_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL5587687.1"
                     /translation="MLNKIQDRFTESIQTQISASELLPAKLSEAANRIVACLLRGDKV
                     IVCGHGRSYGNAELLVSHLLHRYELARPSFAAVQLQLSGVLAGVVAQDNELEAVYKKQ
                     LQAVAKEGDLFIVFSPVGNEECILQAIHTAKNQHLDLDIIALTGSNNDHTLGLLDESD
                     IEISAPSTNQLRVIESHHFYVNLLCELIDHLLFS"
     gene            complement(1884..2255)
                     /locus_tag="PXE44_000133"
     CDS             complement(1884..2255)
                     /locus_tag="PXE44_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDL5587688.1"
                     /translation="MELFNKLINKREQGSFYEQKARVFLEQQGLRFVAANQAFKGGEL
                     DPIMQDGQTVVFVEVRQRKSNRFGSAVESIDYRKQQKWQNAANMWLLKQNQSLETANC
                     RFDVVAFEGNQTPVWIPNFLG"
     gene            complement(2230..3951)
                     /locus_tag="PXE44_000134"
     CDS             complement(2230..3951)
                     /locus_tag="PXE44_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HDL5587689.1"
                     /translation="MATILNHTMKKALVPTAIALFISACTSVNPVTESIKNEAYSSSE
                     FYINKADQTKEAEDKISYQLLAVRKLIDENKEYEAQNTFSEILTAEMNEVQKLEYALV
                     SAQLAALQGKNEQATSQLKAIQDPLLSSAQRLRYIQTQARIAANQKDVIGIVRARSQL
                     NNFYKMNRERQENNDIIWQTLRDANRGMLEKTVPEAGEMELAGWLALINIYNQNVTTP
                     AQMPQAINNWKAQYPSHSAVAVMPTELQGVSNFQQTQLNSVALLLPLSGDAKILGDII
                     KRGFNDAKADDSTAVQTFDTDSSDVNSLISQAKQQGANVIVGPLLKSRVDEMLLSPEI
                     QNVNILALNATENVRNIAQVCYYGLSPESEAQSGAEKIYQDGNSVAIVAAPQDDYGNR
                     SAEAFAKRWRQLTNSDADVRYYNQPLDAVAAIQNAGVSKAALYILGNAEQVLEIKQGI
                     DSSTLKDRLAIYTSSRSNSPNNGIDFYTSMEGVKFSEVPLLADQSSSEYKKAENLANS
                     DFSMMRLYAMGADAWSLATKFNEFRHIPGYKISGLTGELKAGQHCNIERSLSWMQYRN
                     GAIQQAY"
     gene            4025..4897
                     /gene="rsmI"
                     /locus_tag="PXE44_000135"
     CDS             4025..4897
                     /gene="rsmI"
                     /locus_tag="PXE44_000135"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL5587690.1"
                     /translation="MKNRQNSSGINPNYGILYIVATPIGNLGDITQRALDTLSSVDLV
                     AAEDTRHSGLLLSHYGIKKPFFALHDHNEQQKAVVLVEKLAKGENIALISDAGTPLIS
                     DPGFHLVRHCRQAGVKVVPIPGACAAVTALCASGIASDRFCFEGFLPAKTKSRCDKLT
                     ELENEPRTLIFYESTHRILDTLADMQTVFGEDRYVVMAREITKTWETIYGDQLGNLIE
                     WLNEDSNRIKGEIVLVVEGKPQSSEEAFSSQAIKLLTLLCQELPLKKAAAIVAETFGY
                     KKNALYQYGLEHFE"
     gene            complement(5142..6308)
                     /locus_tag="PXE44_000136"
     CDS             complement(5142..6308)
                     /locus_tag="PXE44_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5587691.1"
                     /translation="MTNLLLAIIYLSFISLGLPDAILGSAWPSMHQQLAVPLSYAGVV
                     SMIMSIGTITSSLMSDKLTYHLGTGKVMVISVALTALALWGFSISDAFWILCLWAIPY
                     GLGAGAVDAALNNYVAVHYASRHMSWLHCMWGVGASIGPHILGYALLQGRGWEAGYFT
                     ISMLQIGLTLMLFCSLPFWKLRKQITAEINHSAKPMSLKETLKIPGVKYGILAFFGYC
                     GVEQTAGLWASSYFVMVKDVGLEQAAAFGSIFFIGITVGRGVSGFLTLKFSNRQMIKL
                     GSALILVGIAILCLPLGKYVTLASLIIIGLGCAPIFPCLIHSTPAFFGEDKSQSVIGV
                     QMASAYIGVLLLPPIFGFIANAISIQLLPLYLLLMFAIMAYAYRNLVRETSPSP"
     gene            complement(6460..7974)
                     /gene="putP"
                     /locus_tag="PXE44_000137"
     CDS             complement(6460..7974)
                     /gene="putP"
                     /locus_tag="PXE44_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDL5587692.1"
                     /translation="MFGLDPTTIMFVVYILGMIAIGFIAYRVTNNLSDYILGGRRLGS
                     FVTALSAGASDMSGWLLMGLPGAVYAGGLIEGWIAIGLTIGAYLNWKLVAGRLRSHTE
                     HSGDALTLPEYFHNRFGDKTRLIKILSAVIFLLFFAIYCASGVVAGARVFENLFGLPY
                     HQAIWYGAVATILYTFIGGFLAVSWTDTIQASLMVFALILTPFFVMYNLGGFDEMQAL
                     IDRAGEMAQKDYNDIFTGTSVLGIISLMAWGLGYFGQPHIVVRFMAAESVKSLENARR
                     ISMTWMIICLLGAIGIGYFGMAYFFGNPEQVGNISKNPEQIFIELAKLLFNPWVAGIL
                     LSAILAAVMSTLSAQLLICSSAITEDLYKGILRPQASDKELVWLSRLMVLAVAALAIY
                     IAQDPDSKVFGLVRNAWAGFGSAFGPVVLLSLFWKRMNGFGAIAGMLTGALIVHFWGD
                     ITLKLALPEIYSMIPGFIAATLMIIVVSLITPMPSKAVLDTFDRANKAYLAEVK"
     gene            complement(8068..8856)
                     /locus_tag="PXE44_000138"
     CDS             complement(8068..8856)
                     /locus_tag="PXE44_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL5587693.1"
                     /translation="MNAKKEKESSGNQSLIRGLTLLELLAKFPNGCPLAHLAELSGLN
                     KSTVHRLLQGLQQEGYVRPAPTAGSYRLTTKCLAIGQRALSSINILHIAAPHLEALNL
                     TLGETVNFAMRENDHAILINKLEATTGMMRTRAYIGQQMQLYCSGMGKLFLAYGSADY
                     IPQYWQTKSDIIQKLTVNTITTIDGMQTELETIRRQGFAMDNEENELGVSCIACPIFD
                     HQQKVHYAISVSLSTARLNQLGRENLLPAIQETAEKISAELGGR"
     gene            complement(8853..9431)
                     /locus_tag="PXE44_000139"
     CDS             complement(8853..9431)
                     /locus_tag="PXE44_000139"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL5587694.1"
                     /translation="MNQSSLTDLRSQLRQSMRLKRQSLTAGQQTQAAESIIPQALSLI
                     ESYQASHIAFYLPFNGEISPIPLMEQLLQQGKKLYLPLLHPFTSGQLLFVNYNCKTIL
                     KFNRLGIQEPILDVRNILPLQELEMIFTPLVACDKAGNRLGMGGGFYDRTLAQAPHLI
                     SVGLAHECQQVEQLPIESWDMPLDHIILGAAK"
     gene            complement(9433..9624)
                     /gene="ssrS"
                     /locus_tag="PXE44_000140"
     ncRNA           complement(9433..9624)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PXE44_000140"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(9729..10019)
                     /locus_tag="PXE44_000141"
     CDS             complement(9729..10019)
                     /locus_tag="PXE44_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL5587695.1"
                     /translation="MSANNIELQIFGQVLRLQCPVEQQEHLLASAQRLEERVVVLKEQ
                     SGIIQLEKVLAIVALNLNYELEQAQRQNAENKNVLVACIEQLDNSLGKFQSN"
     gene            10098..10655
                     /locus_tag="PXE44_000142"
     CDS             10098..10655
                     /locus_tag="PXE44_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HDL5587696.1"
                     /translation="MAITDSQQFKQLVSQLQIDDTPAEFHGFLCGLIAGGIQDESWKT
                     LTYQFTNDGHAFSIEPLNTLTDFYQQLSESFSEANTLFSLWLPQNEEDGFALADGISE
                     WTNSFLLGLGVAQPKLQQETDEVGEAIDDLDEIAKLGYSDDDNNEELLEAGEEVLEYL
                     RVLALFLHSHFALGVPKTEEKPPLH"
     gene            10753..11817
                     /gene="hemE"
                     /locus_tag="PXE44_000143"
     CDS             10753..11817
                     /gene="hemE"
                     /locus_tag="PXE44_000143"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDL5587697.1"
                     /translation="MSELKNDRYLKALLREPVDMTPVWMMRQAGRYLPEYKATRAEAG
                     DFMSLCRNADLACEVTLQPLRRYELDAAILFSDILTVPDAMGLGLSFGAGEGPKFARP
                     VASLNDVNQLPIPDPEDELQYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTKIKKMLYAEPHILHKLLDKLADSVILYLNAQIKAGAQAVMVFDTWGGVLAHED
                     YKAFSLHYMHKIVDGLIRENEGRKVPVTLFTKGGGLWLEAIAETGCDAVGLDWTVDIA
                     DARRRIGDKVALQGNMDPSVLYASPERIEQEVKNILAGFGNGSGHVFNLGHGIHLDVP
                     VESPKVFVDAIHNYSKQYHK"
     gene            11964..12554
                     /locus_tag="PXE44_000144"
     CDS             11964..12554
                     /locus_tag="PXE44_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF416 family protein"
                     /protein_id="HDL5587698.1"
                     /translation="MRNPIHKRMERFEPWQNTTFMACLCERMYPNYQLFCEVTEQPEK
                     AKVFQNILNLVWEYLTIKGAKINFDNQLEKLEEIIPDVNEYAFFGVLPAQEACEALSE
                     LLHSIIAGSTLEQAIRISQISLGTVASYLEMQQDKELNEQELKNSEEIQEELDVQWQI
                     YRLLNECEKRDLDLIFELKDEIRSSGISNIGLNINQ"
     gene            12690..12962
                     /locus_tag="PXE44_000145"
     CDS             12690..12962
                     /locus_tag="PXE44_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL5587699.1"
                     /translation="MNKTELIDAIAAGANLTKKDAKAALEATLDAISASLKAGDAVQL
                     IGFGTFKVNERKARTGRNPKTGEEIKIAAAKVPAFVSGKALKDLVK"
     gene            complement(13051..13611)
                     /locus_tag="PXE44_000146"
     CDS             complement(13051..13611)
                     /locus_tag="PXE44_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587700.1"
                     /translation="MKKSFLSLLVLGTTMALAACDQAKEAAQSTTDKAVEVKEAVAEK
                     AAEVKSATEAKVEELKAAANDAAQATADKTEEAKKAVAEKATELKTTTEAKVEELKAA
                     ANDAAQATTDKTEEAKKAVAEKAAELKATTEAKVEELKAAASDATQATADKAEEAKKA
                     VAEKAAEVKVETETKVEEMKKAIESK"
     gene            complement(13757..16699)
                     /gene="rne"
                     /locus_tag="PXE44_000147"
     CDS             complement(13757..16699)
                     /gene="rne"
                     /locus_tag="PXE44_000147"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDL5587701.1"
                     /translation="MKRMLINATQKEELRVALVDGQRLFDLDIESPGHEQKKSNIYKG
                     KITRVEPSLEAAFVDYGAERHGFLPLKEISREYFPSDYVFNGRPNIKDIIQEGQEVIV
                     QVSKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERLELKEALDCLDV
                     PEDVGLIVRTAGVGKSPEELQWDLKVLLHHWEAIKKAAESRPAPFLIHQESDVIVRAI
                     RDYLRRDIGEILIDNKKVFEKAKNHIRLVRPDFINRVKLYEGEVPLFSHYQIESQIES
                     AFQREVRLPSGGSIVIDVTEALTAIDINSSRSTRGGDIEETALNTNLEAADEIARQLR
                     LRDLGGLIVIDFIDMTPVRHQREVENRIREATRQDRARIQFARISRFGLLEMSRQRLS
                     PSLSEASSHICPRCQGTGKVRDNESIALSILRLLEEEAIKENTAQVHTIVPVQIASYL
                     LNEKRKAISSIEKRHDVQIVVVPSESMETPHFSVYRLRENEITPTLSYDLTKHYEARH
                     EEEHPHHHHQEDVLVTRNEPVITVESVLQSSELNLQPSPTPTEPPKPSLLSRLIATIK
                     GWFSEEKVEEKPKPKARNQRERRQRRERPNRNAKAENQERKTPQAQTTPAEEKPMRSE
                     KKARRAVVEEVSGVTEQESRSNSREVQTERRQRRDLRKKVRIETQEVEVENLTPAVEE
                     TVAKQVENVQKNQQIQPLATQEVPVETAPEVETTNEGRENNRQRRLPRHLRVGNQRRR
                     ENQRNQVISPMPLTSAVASPELASGKVWVQVTSTVKVEEKASFLSVDQMLEQQENQLV
                     ETEKKSEEKKLHTVSPLGGFVAQATNRDLVKEKQEQLSKPKEEKVASEDVKPVEVKEH
                     IAPFGSVVAQHSERDLVKEKNERVSKFQSQATVAPAIVEEVTNPIEVPKSTYESDYRF
                     NGRLGTFSRVEHTQAAMTIASAPSDKLEVVEISQWQNSKYYFYGKGFGGYTSAVSQVQ
                     SAPRSTNG"
     gene            complement(17156..18001)
                     /locus_tag="PXE44_000148"
     CDS             complement(17156..18001)
                     /locus_tag="PXE44_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL5587702.1"
                     /translation="MMSQEQFEEINQERRQALNHGSRIAMAASVLGALGIASTANAKQ
                     ASAEKSPYAEPEQYGLKRHGMTIDPKRVALVVVDPQIDFLGPKGVMWGVLGDSIKENN
                     TVANIESLFKAAKAVDMPTFVSPHYYYPTDHKWEFGSPGEHFMHDSGMFARKGQYTME
                     GFENSGADFMPEYKPYIFDGKTVITSPHKIFGPETNDLVLQLRKRKIDQVILAGMAAN
                     LCIDSHLRELIEQGFEVTVVKDATAGPRIPEGDGYLAALTNYRIIANDLWTTEEAVKK
                     MTAKA"
     gene            18128..18952
                     /locus_tag="PXE44_000149"
     CDS             18128..18952
                     /locus_tag="PXE44_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5587703.1"
                     /translation="MDSLTHLFTQFQFRTDLFHLGQLCQIGSFNEENKGYLHFVRQGR
                     FILNQAAERPITIERPAIIFSPTNILHSLHPLDDSGLDIFCINFDFGKGIRNPLTHTL
                     HNIVILELAQYPELGVLADQIFSEVNQKGCGYQAMIHHLCAYFTLKVARQCLEKGYIQ
                     TGLLKGLADKQLGKVLLAIHNAPERDWNVEELANVALMSRSRFAAYFKQTMGVSPMDY
                     LTNWRLAVAQHLLQKGIPVALVAEQVGYSHNAALSRVFMRELGLSPTEWLAQNKES"
     gene            complement(19037..20500)
                     /gene="ubiD"
                     /locus_tag="PXE44_000150"
     CDS             complement(19037..20500)
                     /gene="ubiD"
                     /locus_tag="PXE44_000150"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDL5587704.1"
                     /translation="MKYKNLREFLDFLESQGELVRIQQEIDPYLEMTEIADRTLRKGG
                     PALLFENPKGYQMSVLCNLFGTPKRVALGMGQEDTKALRELGKLLAFLKEPEPPKGFK
                     DLIGQLPQWKQILNMPPKVLSKADCQQVVISGDEVDLYKLPIMHCWPGDVAPLVTWGL
                     TITQGPYKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWKAENPGKPFLVSVAI
                     GADPATILAAVTPVPDTLSEYAFAGLLRGHKTEVVKSISNELEVPASSEIVLEGYIDL
                     EETALEGPYGDHTGYYNEQEYFPVFTVTHITMRKDAIYHSTYTGRPPDEPAVLGEALN
                     EVFIPILQKQFPEIVDFYLPPEGCSYRLAVVTMKKQYAGHAKRVMMGVWSFLRQFMYT
                     KFVIVCDDDVNARDWKDVIWAITTRCDPARDTTLIEHTPIDYLDFASPIAGLGSKMGI
                     DATNKWAGETTREWGTPIEKDDNVVKRIDEIWDSLGI"
     gene            20637..21611
                     /gene="znuA"
                     /locus_tag="PXE44_000151"
     CDS             20637..21611
                     /gene="znuA"
                     /locus_tag="PXE44_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDL5587705.1"
                     /translation="MFKRTLIGLAVLGASVAQADVLTSIKPLGFIASAITEGVTETKV
                     LLPVTASPHDYSLKPSDVTQLQSAQLVVWVGDGLEGFLEKSIEKLPREKVLTLEDVDA
                     IDDIVDKASKKEDKDDDHDHKHEHKHEHKHEHKHEGHNHEGHNHDHHGHSHDEDWHIW
                     LSSEASELAAEQIAARLSEQMPEHKAKIAENLAKFKANLTAKKAEIAKQLEPVKAKGY
                     YTFHDAYGYFEREYGLNSLGSFTINPTVAPGAKTLTAIKKNIAKNKAQCLFAEPQFTP
                     KVIETLAKETKVNVGQLDPLGAKVQLSATAYPEFLQVLADEFSQCLTK"
     gene            complement(21663..21983)
                     /locus_tag="PXE44_000152"
     CDS             complement(21663..21983)
                     /locus_tag="PXE44_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HDL5587706.1"
                     /translation="MIYPTCPECKSENTYHDSIQFVCPDCAYEWTGEETDQDDDVLIV
                     KDSNGNLLSDGDDVLLIKDLKLKGSSEVLKKGTKFKGIRLAQGDHNVDCGKIMLKSEF
                     LKKA"
     gene            22124..24487
                     /gene="rnr"
                     /locus_tag="PXE44_000153"
     CDS             22124..24487
                     /gene="rnr"
                     /locus_tag="PXE44_000153"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL5587707.1"
                     /translation="MIVDPNYQQELEKYENPVPSREFILNTIREHNAPMSRDELLSAF
                     HIDDEERMEGIRRRLRAMENDGELVFTKGKRYALPEKMDLIKGTVIGHRDGYGFLQVE
                     GHEKGATKSEDWFIPNAQMVKVMHGDFVLAQPNGTDRRGRKEVRIVRVLEARKKQIVG
                     RFFLESGIGFVVPDDSRVNQDILIPDEHRMGARMGQVVVVELQERKASFSRPVGIITE
                     ILGDNLAPGMEIEIALRNHDIPHIWPEGVEKQIRQFSSEEVPEEAKQGRVDLRDLPLI
                     TIDGEDARDFDDAVFAKKEGNGWRLWVAIADVSYYVRPKTALDLEALNRGNSVYFPNR
                     VVPMLPEVLSNGLCSLNPQVDRLCLVAEMSVSSKGELQDYQFYEAVMNSHARLTYTKV
                     WKMLEGDEALRERYAPLVPHIEELYAMFQTLMKARHKRGAIEFETIENQFIFNPQGRI
                     ERIEPLIRNDAHKLIEECMILANIAAARFVEKANEPALYRIHDKPSEEKLLSFKSFVR
                     ECGLTWDVGLDPTPKDYGVLLEQIAERADKELIQTMLLRSLKQAVYAADNIGHFGLAL
                     TEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRYYTDGGGYHYKLDDMDEFGDKCSA
                     TERRADEATREVADWLKCEFMQDHLGEEFDGVISSVTGFGLFVKLNELLIDGLVHIST
                     LDNDYYHYDADRQRLIGGSGIIYRLGDAVRVKVINVNLDEKKIDFELLTGNKKTGKTA
                     KKKAKATEVPVFKEPKTVKKAKKVAEKKPAKSAKKRTKTTASKSRKK"
     gene            24491..24757
                     /locus_tag="PXE44_000154"
     CDS             24491..24757
                     /locus_tag="PXE44_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587708.1"
                     /translation="MDYKKQILFSLLFALVIAIISYFLFTYIPFDCSDLSKLNDSQRL
                     ECRTAEFLGVYYWLPHTTTLFFGSVLLYWAVYGMISLVQAVLKR"
     gene            24980..25438
                     /gene="mraZ"
                     /locus_tag="PXE44_000155"
     CDS             24980..25438
                     /gene="mraZ"
                     /locus_tag="PXE44_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL5587709.1"
                     /translation="MFRGASSISIDSKGRIAIPTRYRAELLEKHHGILVCTVDIRQPC
                     LLLYPLHEWEMVEQKLLALSNFDPVQRRIQRVMQGFATECEMDSAGRILLSPTLRQHA
                     QLEQQIMLVGQLNKFEIWQDKQWQAQIAEDLAFGSSAEILDCEALKNLSL"
     gene            25454..26392
                     /gene="rsmH"
                     /locus_tag="PXE44_000156"
     CDS             25454..26392
                     /gene="rsmH"
                     /locus_tag="PXE44_000156"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL5587710.1"
                     /translation="MSENPKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSK
                     LGENGRLIATDRDPRAIAEAQTISDPRFQIEHTAFSAIPELCERMGLVGKIDGILLDL
                     GVSSPQLDEAERGFSFMRDGPLDMRMDTTKGLSAAEWLAQVSAEDLAWVLKEFGEERF
                     AKRIAQAVVSYNKSANEKISRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINGEL
                     DELEKALNSALTVLAPEGRLSIISFHSLEDRMVKQFVRKHSKGMDVPRGLPILEAELT
                     KNIPLKTIGKAIMPSEAEIEANARSRSAVLRIAEKR"
     gene            26411..26722
                     /gene="ftsL"
                     /locus_tag="PXE44_000157"
     CDS             26411..26722
                     /gene="ftsL"
                     /locus_tag="PXE44_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL5587711.1"
                     /translation="MSNERYPLHQIIIDDIFSHNKVALLLLIGVVISAVATIWITHQT
                     RLSVSEQGQLIQANQKLESQYTNLQLEENSRSSRARVDAAAKSFGLQPIKKEQEIILV
                     E"
     gene            26726..28786
                     /locus_tag="PXE44_000158"
     CDS             26726..28786
                     /locus_tag="PXE44_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HDL5587712.1"
                     /translation="MVKSVKLKRKQEAPVAKAEPQQAEGKKTPSFLPTRFRVVVFFML
                     LMVGGLLAQTAYIQLFDSERLIKEANNRSLRTKELQFTRGRILDRNGRFLSISVPMYS
                     LTLDPREYFDTKLRRSNESWRALAIEMETSKGKIEANVNKFIEKKNFSKEKVDFDPRS
                     ILNTKSEGYWTQLAQVTGLDYNSLIEKVRNNPSSVFLRLDNENLVRERQKFQALSKEI
                     GRSYNDIMDELYSKNRQRFLYLSRHQSEAISEYAKELDIDALVIKVESRRFYPLAEES
                     SQLIGFTDKDDMHGSEGLERSFDSLLIGKNGKQIIRKDAKGNIVENIRSEKQYDPQDV
                     MLSIDEELQSMVYGEIKKAVQENNAESGTAVLVDVQTGEILAMANAPSFNPNKRDSFK
                     PELMRNRAITDTFEPGSTVKPFTVLTALQNGVTYRDEVIGTRPFVVNGHTIKDVAPRD
                     SLTLTGILQKSSNIGVSKLALRMPANALVDTYTKVGFGKDTGLGLGEQRGTNGDRKRW
                     ADIERATLSYGYGLNVTPLQLARAYATLGSFGLYRPLSITKVDPPVIGERVLPEKTTR
                     DVVHMMESVAAKGEGGHRAAVDGYRVAIKTGTARKLEKGKYVEKYIAYTAGLAPASDP
                     RFALVVLINEPKAGNYYGGFVSAPLFSRIMGYTLKQRNIKPDNLTENTQSAVREIKLE
                     RPIN"
     gene            28795..30285
                     /gene="murE"
                     /locus_tag="PXE44_000159"
     CDS             28795..30285
                     /gene="murE"
                     /locus_tag="PXE44_000159"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDL5587713.1"
                     /translation="MKRLLPFLTELDAWVEELTELKQMTLDSRQVQAGDLFVALKGHQ
                     VDGRKFIPNAIEQGAAIILAEADEDQPEIELDPKFAKFNLDRTACCKVVSVPRLPKLL
                     SEIAGALYHNPSHKLTLAGITGTNGKTTTAQLLAQWHNLLGGKSAVMGTIGNGLYGQV
                     QEAVNTTGSAIEIQHNLANFVELGADFCAMEVSSHGLAQFRAEALDFDLAIFTNLSRD
                     HLDYHNTMEEYAQAKFRLFNELSTKAQVINADDEIGREWLTQLPNAVAVSTDPKFAGN
                     HQFVKATAVKFTLQGASIAFESSWGNGELHSRLIGAFNVNNLLTAFAGLLALGFDIQE
                     LVKTAPNLVGVAGRMECITAPNKPMVIVDYAHTPDALEKALQAARLHCEGELWCIFGC
                     GGDRDAGKRPLMATIAEKLTDKVIATDDNPRTEDNQKIMADIVKGFSKPQQIIHNREE
                     AIKTAIEQAKATDVILIAGKGHEDYQIIGTEKLHFSDQETARKYLF"
     gene            30373..31767
                     /gene="murF"
                     /locus_tag="PXE44_000160"
     CDS             30373..31767
                     /gene="murF"
                     /locus_tag="PXE44_000160"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL5587714.1"
                     /translation="MIKLTTQQIAEILNAKLIGNGETVVETTSTDTRQAVENGLFFAL
                     KGENFDAHHYLQNAVAQGCVAVVVERECEIDVPQLVVADTRLALGELAKWLKAKLNPK
                     TVAMTGSSGKTTVKEMTAKILQKMTACEQEVLYTFGNLNNDLGVPMTLLRLTEQHKYA
                     VIELGANHIGEIAYTTAIAQPDACLVNNVAAAHLEGFGSLAGVAQAKGEIYRGLKENG
                     KAIVNLAFYYPQWQKEIGSREMQSFSYIGSDSDSYADYWAEDVELHLSGSRFTLHTPQ
                     GEIAIDLPYLGKHNVSNALAATSLAMSVGATLEAVKAGLEQRSMVKGRLYPVQINDHC
                     LFIDDTYNANVDSMKSAISVLENYPAYRIFAVGDMAELGEESSKCHQEVADFAKNAAL
                     DLIVSFGKESAVISQQAEHHFTDKQQMVDFLLPIILQKIEQKQPLVLLAKGSRSQKME
                     TVISELCKPFNLNF"
     gene            31788..32870
                     /gene="mraY"
                     /locus_tag="PXE44_000161"
     CDS             31788..32870
                     /gene="mraY"
                     /locus_tag="PXE44_000161"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL5587715.1"
                     /translation="MLVWLAELLVQYNTAFNVVSYITFRAIMALLTAMGIGLWIGPKV
                     IRRLQILKFGQEVRNDGPESHFKKRGTPTMGGVMILAAIGVSTLLWADLRNSYVWFTL
                     FVLFGYGAVGFVDDYRKISRKNTDGLIARWKYFWLSAIALVAVFGMYAVGKDTAATQL
                     VVPFFKDVMPQLGLFYIVLSYFVIVGTSNAVNLTDGLDGLAIVPTIMVASAFALIAWA
                     TGNFNFAQYLHIPFIPNAGELVILCTAIVGAGLGFLWYNTYPAQVFMGDVGSLSLGGA
                     LGVIAVLVRQELLLVVMGGVFVVEALSVILQVGSYKLRQKRIFRMAPIHHHFELKGWS
                     EPRVIVRFWIITLMLVLVGLVTLKLR"
     gene            32934..34235
                     /gene="murD"
                     /locus_tag="PXE44_000162"
     CDS             32934..34235
                     /gene="murD"
                     /locus_tag="PXE44_000162"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL5587716.1"
                     /translation="MQKQYENKTVTIIGLGTTGLSCVDFFKKKNANIQVIDTRANPAG
                     ADKLDVTIPLHTGGLNSEWLLKSDLIVISPGLAVATPEIQQAIQAGVEIVGDVELFCR
                     EAKAPIIAITGSNGKSTVTTLTTEMAKQAGIKVGMGGNIGVPVLSLLEGDYDLFVLEL
                     SSFQLETTYSLKAKAATVLNISEDHMDRYDSIADYRQAKLRIYDNAENIIVNGEDAQT
                     YPIQAVKKVIRFAEQNAEYSLRNQMLYSGDDAIIQTKQMLISGRHNEMNALAAMALAE
                     AANVPREGIVKALQIYGGLPHRFQAVATNDGVRWVNDSKATNVGSTVAALNGLNIAGT
                     LYLLLGGDGKAADFSELKPLINKPNIVCYCFGQDGAALAELSEQSVLVETMQQAIEAI
                     RPQLKKGDMVLLSPACASLDQFKNFEQRGEVFMALAQGQHA"
     gene            34235..35413
                     /gene="ftsW"
                     /locus_tag="PXE44_000163"
     CDS             34235..35413
                     /gene="ftsW"
                     /locus_tag="PXE44_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HDL5587717.1"
                     /translation="MLERIKSEWDKWVRLTPSNSLYDRTLIWLFLGLLIIGFVMVTSA
                     SLPVSTRLNNDPFYFAIRDGLYIIASIIFCYVFVQIPIEKWEKHNLALFFISIGFLIA
                     VLIFGRSINGAVRWIPLGILNFQPAELAKLAVICYFASFYVRKYDEIRKEKASFWRPA
                     VILFLFGFLLILQPDLGSTFVLFVLTFSMLFIVGAKIMQFMFLGVVGTVLFAVLILTS
                     EYRLKRVTSFMDPFADAYGDGFQLSNSQMAFGQGEFWGQGLGNSVQKLEYLPEAHTDF
                     VMAVIGEEFGFFGIACIVLLLILLTVRALKISKESLVLEERFKGYMAFGIAIWVFLQG
                     FVNLGVASGLLPTKGLTFPLVSYGGSSLVIMSIAIAVLLRIDHENRAERIGHAKIKTI
                     "
     gene            35532..36587
                     /gene="murG"
                     /locus_tag="PXE44_000164"
     CDS             35532..36587
                     /gene="murG"
                     /locus_tag="PXE44_000164"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL5587718.1"
                     /translation="MAKKLLVMAGGTGGHVFPAIAVARELQKQGWQIRWLGTKDRMEA
                     TLVPKHGIEIDFIEISGLRGKGVAALLKAPFAIFKAVMQARKIIKNYQPDAVLGMGGY
                     VSGPGGIAAKLCGVPVILHEQNAVAGLTNVWLSKIARKVLQAFPTAFKEAEVVGNPVR
                     EDLSALPLPNERFAKRGYPINILVMGGSQGARVINQTVPEVAKVLGNNVFISHQVGKG
                     NLAGIEEVYQQTGNGIASEFIDDMKAAYEWADLVICRSGALTVCEIAAVGLPAVFVPF
                     QHKDRQQFLNANYLAADGAAVIIEQSDFTPKALQQALEPLIANRQLLLDMSMRARAKS
                     TPMAAKRVAEVIIQEAN"
     gene            36663..38090
                     /gene="murC"
                     /locus_tag="PXE44_000165"
     CDS             36663..38090
                     /gene="murC"
                     /locus_tag="PXE44_000165"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL5587719.1"
                     /translation="MKNFQDKVKKLVPEMRRVSQIHFIGIGGAGMSGIAEVLLNEGYQ
                     ISGSDIADRAVTQRLSAAGAKVFIGHQAENIAGASVVVVSTAIDETNPEIIAAREARI
                     PVIRRAEMLAEIMRFRHGIAIAGTHGKTTTTAMISMIYTEAKLDPTFVNGGLVKSTGK
                     NAHLGASRYLIAEADESDASFLHLQPMVSVVTNIEPDHMDTYGGDFEKMKETYVRFLR
                     NLPFYGLAVMCADDETVMEIAPRVGRQVITYGFSENADYRIEDYQQTGFQEHYTVVCP
                     SGERIDVLLNVPGMHNALNATAALAVAKEEGIGNEAILAALADFQGAGRRFDQLGSFI
                     RPNGKIMLVDDYGHHPTEVDVTIQAARSGWEGKRVVMIFQPHRYSRTRDLFDEFVQVL
                     SKVDALILLDVYAAGEAPIVGADSKALARSIRNLGKVDPILVSDTDQLGEVLDQIIQD
                     GDLILAQGAGSVSRISRGLAESWKN"
     gene            38180..39091
                     /locus_tag="PXE44_000166"
     CDS             38180..39091
                     /locus_tag="PXE44_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL5587720.1"
                     /translation="MSIKNEKIAVLFGGVSQEREVSLNSGAAVTAALKSLGYNVEGID
                     TKEFPIEKLKEKGIQRVFNILHGGIGENGVLQGALEQMGIPYTGCGVMASAVTLDKFR
                     TKLLWNAVGLPTADMVVVQRGQAVDFDQIIAKLGLPVFVKSSCEGSSVGVFKVKTKEE
                     LAPAIHEALKFDSIVLVEEFLAGNEYSVPVLDGQVLPAVQVIPDGEFYDYHAKYVSDN
                     TQYVVPALNDERQAEVAKLVKAAYDVVGCRGWSRIDVMEDGEGKFRLVEVNTNPGMTS
                     HSIFPKSAATVGISFEKLVERVLELSA"
     gene            39091..39882
                     /locus_tag="PXE44_000167"
     CDS             39091..39882
                     /locus_tag="PXE44_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL5587721.1"
                     /translation="MGIFRKKNSTNVRLNVLNPKTPVSPRKWLVFIKPLIVLLCTLFV
                     FGVYSNWQNILESWDKTPIRAYALTHKTQFTTNADIRETLSKEPALKGYFGQNIQEVK
                     DKLLSIPWVKDVAVRKLYPDRLSITLLEHKPVAIWNDTNFVSEQGTVFSLPKDRIDKN
                     GLPLLYGPDTEGKSVLEAWNKIRADLNARNLDLTSVSVDNRGSWTIRLSNQVELRLGR
                     GEWTSKIDRFVTIFPEIHIPEGNRLAYVDLRYPHGAAVGFSPIKN"
     gene            39905..41176
                     /gene="ftsA"
                     /locus_tag="PXE44_000168"
     CDS             39905..41176
                     /gene="ftsA"
                     /locus_tag="PXE44_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL5587722.1"
                     /translation="MTKAVESKIIVGLDIGTSKVVAVVGEVLPDGVINVMGAGVCPSK
                     GVDRGGVIDLDAAVNSIQRAIEQAESIADCQIMGVTLAISGQHIVGLNEAGSSTLSGG
                     IVTQDNIESAVDMAKAIKLQDGLETLHMIPQEYHVDRLPATKNPIGLSGMRLQVQAHL
                     IACHQAWLKNLKNAVEHAKLKVDQVVFSGLASSYSVLTEDEKELGVCLIDIGGGTMDV
                     LVYTDGALRYSKVIPFAGNNITDYLARVFTTSRPEAESLKVGYGSAISPPTHNSDKKI
                     EVAGLGGRMARTFTRAQVATVTSQCYNDLLKVVEEELTQLRHELFQKGIKQELIAGVV
                     ITGGGSQIEDIVECAKSIFGSQVRVGYPLNITGLTDYVNKPQYATALGLLQYSHYNYT
                     EQGTSTDSNEDIFAPIGRGTKKIIDFFKSKF"
     gene            41209..42456
                     /gene="ftsZ"
                     /locus_tag="PXE44_000169"
     CDS             41209..42456
                     /gene="ftsZ"
                     /locus_tag="PXE44_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL5587723.1"
                     /translation="MFEPIIEQTQDAIIKVVGVGGGGGNAVDRMSRSADDIKGVEFFD
                     VNTDAQVLRKRTTRQTIQIGASTTKGLGAGADPMVGKQAAEEDREAIANALKGANMTF
                     IAVGMGGGTGTGAAPVVAQIAKEQGSLTVGVVTKPFRFEGPRRMRFADQGIKELSQYV
                     DSLIIIPNDKLRGLGKQTTAVDAFAAANDVLSNCVLGITNMITSSGGSTGADINVDFA
                     DVRTVMSGKGHAMIGTGFAEGEVGEGRAEKAMNDAISSPLLENVDISGASGMLINISA
                     GTDFLLEEVYAMMDLIYGFATEDAAIVFGCNYYPEMDGKVSVTLVATGIGQPEEALHM
                     PHKAQPVYAQQGNHVQATQPTQPNHAVQQPNFTQPSSYGQPNQFSGQPQPLQQPVAPK
                     PQSVDTAQIFNPNSIPGFMRNSQ"
     gene            42488..43408
                     /gene="lpxC"
                     /locus_tag="PXE44_000170"
     CDS             42488..43408
                     /gene="lpxC"
                     /locus_tag="PXE44_000170"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDL5587724.1"
                     /translation="MTKQRTLKQAAKVTGIGLHSGKKVTLTLRPAPANTGVIYARTDL
                     EPVVYFPASADSIRDTQLCTCMVNDDGVRISTVEHLNAAMSALGLDNVIVEVDAPEIP
                     IMDGSSSPFIYLLLDAGIEEQDAPKKFIRIKETVRVEEGDKWAELKPYNKGFKLNFTI
                     DFEHPLIPKNVGSYSIDFSAENFIQQLSRARTFTFMKDVEYLQSIGLALGGSLDNAIV
                     LDDYRILNEDGLRFKDELVRHKMLDSIGDLYMCGYNILGEFSAYKSGHGVNNKLLRAV
                     LENKNAWEFVTFEDKAEVPQGYQVQQEIFI"
     gene            43581..45263
                     /gene="recN"
                     /locus_tag="PXE44_000171"
     CDS             43581..45263
                     /gene="recN"
                     /locus_tag="PXE44_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL5587725.1"
                     /translation="MLTQLTINNFAIVRHLILELNEGMSVITGETGAGKSIAIDALGL
                     CLGYRSESGMIRNGSDKADISATFTMQPHSPAYLWLQEHELLDEDNPQECILRRMINI
                     DGRSKAFVNNRSLPVSQLRELGQYLIHLNGQHAPQLLLKNEYQLEIVDHYATLQPLLM
                     KMAKQYGNWKELNKQVKNFQKQCQENEARKQLLEYQVEELDEFAIKANEFEEMEEKYN
                     ILSSSEELTELSDEITRLLSDDEINANSLIYKALRNAENLAEVDSQYQGILDMLNEAL
                     IQIQEASSEVAQLASKIEQDPELLQELDNRIAQTISLARKHHIQPNELWQYHATLKAE
                     LQELLDFEDNEEQLIQNEKQAYQECITLAEEIYQKRLDATHKLSEQVTKQIKHLAMEN
                     GEFFIHIEHNLEKMSANGADYVEFNLRSNLGQQPQPLAKIASGGELSRISLAVQVLTA
                     NKLSTPTIIFDEVDVGISGSAATTVGKLLRKLGEKCQVLCVTHLPQVASYGNQQFNVQ
                     KYVENNQTETQMTLLTEAERIHALARLLGGSEITETVLANAKEMLNLAKNNE"
     gene            complement(45310..45385)
                     /locus_tag="PXE44_000172"
     tRNA            complement(45310..45385)
                     /locus_tag="PXE44_000172"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45350..45352),aa:Ala,seq:ggc)
     gene            complement(45465..46904)
                     /gene="gltX"
                     /locus_tag="PXE44_000173"
     CDS             complement(45465..46904)
                     /gene="gltX"
                     /locus_tag="PXE44_000173"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL5587726.1"
                     /translation="MKIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKH
                     NDGEFVLRIEDTDLERSTPEATAAILEGMEWLDLAWEHGPYFQTKRFDRYNQVIDQML
                     EAGTAYRCYCSKERLDEVRAEQEANKEKAKYDQHCLHNPQNDPSQPHVVRFKNPTEGS
                     VVFDDAVRGRIEIANSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNT
                     PRQINILKALGAPIPTYAHVSMINGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLV
                     RLGWGHGDQEIFSRDEMIELFDIHSVSKSASAFNTEKLQWLNQHYIRSLAPSYVAKHL
                     EWHMQTQGIDFTSGPKLEEIIPVLGERCKTLKELAESSRYFYQEFKAYDEKAAAKNFK
                     AEAVAPLAKLLEKLTACNDWTVENIHEAMNQTAAELEIGMGKVGMPFRLAVTGSGQSP
                     SMDITAKLVGKERTLARLQKAIEFIQANS"
     gene            47079..47154
                     /locus_tag="PXE44_000174"
     tRNA            47079..47154
                     /locus_tag="PXE44_000174"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47112..47114,aa:Val,seq:tac)
     gene            47203..47278
                     /locus_tag="PXE44_000175"
     tRNA            47203..47278
                     /locus_tag="PXE44_000175"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47236..47238,aa:Val,seq:tac)
     gene            47331..47406
                     /locus_tag="PXE44_000176"
     tRNA            47331..47406
                     /locus_tag="PXE44_000176"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47364..47366,aa:Val,seq:tac)
     gene            47455..47530
                     /locus_tag="PXE44_000177"
     tRNA            47455..47530
                     /locus_tag="PXE44_000177"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47488..47490,aa:Val,seq:tac)
     gene            complement(47684..49177)
                     /gene="mltF"
                     /locus_tag="PXE44_000178"
     CDS             complement(47684..49177)
                     /gene="mltF"
                     /locus_tag="PXE44_000178"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDL5587727.1"
                     /translation="MKGLIARLFIAVALLLWAWDMIFPWQKIIHSEANHYTQIQQSKT
                     LKVGMINHPLSYFVGPEGKSGIEYDLATAFAQYLKVNLDIKTYDSSEKLFEALRNNDV
                     DIAAAGLLYHSELNDEFQIGPSYYSASWQLVYKKGSARPYRLSDLRENVIIPAGSAVI
                     PILNQLKVDNPELKWQISDKFTQEELLLKVAQGEISHTIAMSVDISAAQHINPNLAVG
                     FDLTDENPVLWYLANSSYSELQSVLLDFMSYSMETGLISRIEEKYFNHLAKFDYVDIQ
                     SYLNAIKTILPKYEPLFRAHKGELEWQMLAAIAYQESHWDPNATSPTGVRGIMMLTKD
                     TADRMKISDRTNPAQSIKAGSEYLHMLMRQMPETIPQADRIWYSLAAYNMGLGHLLDL
                     RRLTKQLGGDPDNWLDVKKNLPLLAEKRYYANLKYGYARGYEALQYVENIRRYYGSII
                     NYQRVEEQKMQEQENLPDGQIKQENSSYEKIEEKEIPTDKEITPASN"
BASE COUNT        14461 a        10011 c        10627 g        14203 t
ORIGIN      
        1 tctcatagac aatacgataa ttgcggcaga atgtttctaa tgtgccatct tctctacgtc
       61 gccctattgt aggcataaat gcaatgagat cgagtttatt ttgtaattgt tcccaaagtg
      121 aaactgcgct caaccaatgc cctgtataat cttctaccga ctgtaaaatc tcaatgacat
      181 tagaccttgc ggtaagtgac agctcccaag atttaggcat aattaacctc aaacaaattt
      241 tcattttctc gtaattgctg acgtttctgc atatccgcaa ataaatgatt taattcgtta
      301 ttgatttgtt ctttggtaaa ggtttctcct cgagctattg cttcttgacc agctttaatt
      361 tgctctgcaa gaaactcatc atatccttgt tctttgaata gcatattctg ctcctttgat
      421 ttgtgtcttg attccactat gttcaagaca tagtttaagt ttaaatctac gctcaaagca
      481 atgaagagtc aagggaatcc ttatgttgat agggtgtgca tatttttcga gtcaaatgaa
      541 ttaaaaatca tcattacgat cacccactca tagattttat cttcctatct aaggtcatct
      601 tcttatatca aacaagcggt ctaaatttgc aaaaaaatga caaatttaga ccgcttgtga
      661 gttacaaaaa tataaactaa ttgagcttat caattactgc gcataactaa atactttcac
      721 cactttggtt actccaccta cattgcgagc ggcttcggcg gcggcatcag cttggttttg
      781 gctgactttg cccattaaaa acacttcgcc gttttcggtg acgactttca cttcggtggc
      841 ttttacctcg ctatttacta acagtttgga tttcacttgg gtggtaatcc agctgtcttg
      901 tgtaatctgt ccaaagccga ttttttcgcc ttggcggatt tcgttgtaaa tttccgtcac
      961 gccctccgcc ccagcagcga ggtttttcgc attttccact acgtccatac ttggtgcttg
     1021 cccgattaat aacactttgc cgttgtaagc caccacgtta atgcgggctt cttgtttgat
     1081 ttgctcgtct ttattaatgt tgtaagcgac tttttcttct aaaatttcat catcaacttg
     1141 cgtgccggtg gtgcgtgggt ctgtcgccac tttagttgca accgccgcag aagtcaccac
     1201 cgccgtggtg atacaacctt gtaaagagat aaaacctaat actaataggc tgccctttaa
     1261 tagtttattt gctaatttgt tcataatctc tccttacgaa aatagtaaat ggtcgataag
     1321 ttcacataat aaattcacat aaaaatgatg gctttcaatc accctcagtt gattggttga
     1381 aggggctgaa atttcaatat cactttcatc taataagccg agtgtgtggt cgttattact
     1441 gcctgttaaa gcaataatat ctaaatctaa atgttgattt ttcgccgtat gaatagcttg
     1501 caatatacat tcttcattac ccacaggcga aaaaacaatg aagagatcac cctcttttgc
     1561 aaccgcctgc aactgttttt tataaacagc ctctaactcg ttatcttgtg ccacaactcc
     1621 tgccaataca ccgcttaatt gcagttgtac ggcagcaaaa ctcggtcttg ccagttcata
     1681 acggtgaagt aagtggctga ccaataattc ggcattaccg taagagcgac cgtgtccgca
     1741 gacgataact ttatcgccac gcaacaaaca ggcaacaatg cggtttgccg cttcacttaa
     1801 tttagccggc aatagctcag aggcggaaat ttgggtttga atactttcgg taaatctatc
     1861 ttggatttta tttaacattc tttctaacct aaaaagttgg gaatccacac aggcgtttgg
     1921 tttccttcaa aagcaaccac atcaaaacgg caatttgcag tttctaaact ttggttttgt
     1981 ttaagcaacc acatattagc ggcattttgc catttttgtt gcttgcggta atcaatactc
     2041 tcaacggctg aaccgaagcg gttgctttta cgctggcgaa cttctacaaa cacaacggtt
     2101 tgtccgtctt gcataatcgg atccagttcg ccacctttaa atgcttggtt agcggcaaca
     2161 aaacgaagcc cttgctgttc taaaaacact cgggcttttt gttcataaaa cgagccttgt
     2221 tcccgtttat taataagctt gttgaatagc tccattgcga tattgcatcc aagataaact
     2281 acgttcaata ttacaatgtt gtccggcttt taactcaccg gttaaacctg aaattttgta
     2341 gcccggaata tggcggaatt cattgaattt ggttgccaat gaccacgcat cagcccccat
     2401 tgcgtataaa cgcatcattg agaaatcgct attggctaaa ttctcggctt ttttatattc
     2461 actagaactt tgatcagcta acagtggcac ttcactaaat tttacccctt ccattgaagt
     2521 gtaaaaatca atgccgttat ttggcgaatt actgcgagat gaggtataga tcgctaaacg
     2581 atcttttaag gttgagctat caataccttg tttaatttct aatacttgct cggcattccc
     2641 caaaatatat aaagctgctt tgctcacacc tgcattttga atagcggcaa cggcatcaag
     2701 cggttggttg tagtaacgca catcagcatc gctattggta agctgacgcc agcgtttggc
     2761 aaaggcttct gccgagcggt tgccgtagtc atcttgaggg gccgcaacga tagcgacact
     2821 attaccgtct tgataaattt tttccgcccc tgattgtgcc tcagattcag gagataaacc
     2881 gtagtaacac acctgagcaa tattacgcac attttcagtg gcattcagtg ccaaaatatt
     2941 cacgttttgg atttctgggc taagtagcat ttcatcaaca cgagatttga gcaatggccc
     3001 gacaatcaca tttgcccctt gctgttttgc ctgtgaaatt aaggaattaa catcagatga
     3061 atcggtatca aaagtttgca ctgcggttga gtcatcagcc ttagcatcat taaaaccacg
     3121 cttgataata tcacccaaaa tttttgcatc accacttaga ggcaatagca atgcgacact
     3181 gtttaactgg gtttgttgga aattactcac cccttgtaat tccgttggca ttacggcaac
     3241 cgcactatgg ctcgggtatt gggctttcca attattaatt gcttgtggca tttgtgccgg
     3301 tgtcgtcaca ttttgattat agatattaat taatgccaac caaccagcaa gctccatctc
     3361 acccgcttca ggcacagttt tttccagcat tcctcgattt gcatcacgca aggtttgcca
     3421 aataatatcg ttattttctt ggcgttcacg gttcatttta tagaaattgt taagttgcga
     3481 tcttgcacgc acaatgccaa tcacatcttt ctgattggca gcaatacggg cttgggtttg
     3541 aatataacgc aaacgctgtg ccgaacttaa tagcggatct tgaatggctt ttaattggct
     3601 tgttgcttgc tcgtttttcc cttgtaaggc ggcaagctgg gcggaaacca aggcatactc
     3661 taacttttgc acctcgttca tctctgcggt cagaatttca ctaaaggtat tttgagcctc
     3721 atattcctta ttttcatcaa ttaatttgcg tacagctaaa agttgatacg aaattttatc
     3781 ttccgcttct ttggtttgat ctgctttatt aatatagaat tccgagcttg aataggcttc
     3841 atttttaatg gattcggtga caggatttac cgatgtacag gcagaaatga ataatgcaat
     3901 cgccgttggc accaatgcct ttttcattgt atggtttaaa atagttgcca ttgtcagatc
     3961 ctcaatagaa tataatgtgc taaatttaat gtgaaatctt acttatcaaa cgagctaaaa
     4021 gcaaatgaaa aacagacaaa attcatctgg aatcaaccca aattacggaa ttttatatat
     4081 tgttgccacg cctatcggca atttaggcga tattacccaa cgggcgttag acaccctttc
     4141 atctgtcgat ttagtcgccg ccgaagacac tcgccacagc ggtttattgc tgagccacta
     4201 cggcattaaa aaaccgtttt tcgccttgca cgaccataat gaacagcaga aagcggtcgt
     4261 tttagtcgaa aaattagcaa aaggggaaaa cattgcgtta atttccgatg ccggaacgcc
     4321 actgattagc gacccgggct ttcatcttgt ccgtcattgc cgccaagccg gggtaaaagt
     4381 tgtgccgatc cccggggctt gtgccgctgt tactgcctta tgtgcttcag gcatagcttc
     4441 agaccgtttt tgttttgaag ggttcttacc ggcaaaaaca aaatcccgtt gcgataaact
     4501 taccgagctt gaaaacgagc ctcgcacact gattttttat gaatccaccc accgtatttt
     4561 agacactctt gccgatatgc agactgtttt tggcgaagat cgctatgtgg taatggcgcg
     4621 tgaaatcacg aaaacgtggg aaaccattta tggagatcaa ctgggtaact taattgaatg
     4681 gctaaatgag gatagcaacc gaattaaagg cgaaattgtt ctggtggttg aaggtaaacc
     4741 gcaaagtagt gaggaagcct tttccagcca agcgatcaaa ttactgacat tactctgcca
     4801 agaacttccg cttaaaaaag ccgctgccat tgtggcagaa accttcggct ataagaaaaa
     4861 tgccctgtat caatatggat tggagcattt tgagtaacag acaagcggtc agattttgtg
     4921 agttttttgc agattggcac tcccctctct ctgaaaaatg tttctgaacg aaacatttcc
     4981 cggtctcaat actcgctacg ctcgctcctt cggagccgtt ggcaaagcca acgttcaaat
     5041 gtaaacattt gtcccataag gggagagagc taaattattc gcttgtcgac ccctctccac
     5101 tcgtgggaga gggacagatt cttcttcgtt cagaagaaaa atcagggaga gggggaagtt
     5161 tctcgcacta aattccgata agcatatgcc ataatcgcaa acatcaacag caagtaaagc
     5221 ggtaataatt gaatgctgat agcattagca ataaagccga aaatcggtgg caataacagg
     5281 acgccgatat aggcacttgc catttgcacg ccgatcaccg attgagactt gtcctcgccg
     5341 aaaaacgcag gggtcgaatg aattaagcac gggaaaatcg gggcacaacc tagcccgata
     5401 ataattaaac ttgccagcgt aacatatttg cctaacggca agcacagtat cgcaatgccg
     5461 actaagatca aggctgaacc aagtttaatc atttgtctgt tgctaaattt aagcgtcaag
     5521 aagccactta cgcctctgcc aacggtaatg ccgataaaga aaatactgcc gaacgcagcc
     5581 gcttgttcta agccgacatc tttaaccatt acaaaatagc tactcgccca cagtccggcg
     5641 gtttgttcta cgccacaata gccaaaaaaa gctaaaatgc catatttcac gcccggaatt
     5701 tttagcgttt cttttaagct catcggtttt gccgaatggt tgatttcagc ggtaatctgc
     5761 ttgcgtaatt tccaaaaagg cagtgagcaa aacagcatta gcgttagccc gatttgtaac
     5821 attgaaatgg tgaaatagcc tgcctcccag cctcgccctt gcaataaggc gtagcctaaa
     5881 atgtgcggcc cgatagaagc tccaacgccc cacatacaat gcagccagct catatggcgg
     5941 cttgcgtagt gaactgctac atagttattc aacgccgcat caaccgctcc agcccctaag
     6001 ccgtaaggaa ttgcccataa acataagatc cagaaagcat cactgattga aaagccccac
     6061 aatgctaaag ccgttaaggc aacgctgatg accattactt tgcctgtgcc gagatggtag
     6121 gtcagtttgt ccgacattaa gctagaggta atagtgccaa tcgacataat catcgagacc
     6181 acaccggcat aggaaagcgg aacagcaagc tgttggtgca ttgacggcca cgccgagcct
     6241 aaaattgcat caggcaaccc aaggctgata aaagagagat aaataatagc gagtaggagg
     6301 ttagtcatag cttacaatcg gtcacaaaaa gcatcgcttt ttgaacgttg gctctgccaa
     6361 cgggagcgaa gctgtgaaca atccttacaa ggattgtgaa taatttttga taaatttttt
     6421 gtaaaaaaat ggcttaagga gaccttaagc caaattttat tatttcactt ctgccaaata
     6481 ggccttatta gcacggtcaa aggtgtctaa tacggctttg cttggcattg gcgtgataag
     6541 tgataccaca atgatcatta gggtagcagc aataaagccc gggatcatcg agtaaatttc
     6601 cggtaaggcg agcttaagtg taatatcgcc ccaaaaatgc acgattaacg caccggtcag
     6661 cattccggca atcgccccaa aaccgttcat acgtttccag aatagtgaaa gcaataccac
     6721 aggcccaaat gccgagccga aacctgccca cgcattacgc actaagccaa atactttgct
     6781 gtccgggtct tgggcaatat aaatagctaa tgctgccacc gcaagcacca tcaagcggct
     6841 taaccaaacc agttctttat cggaggcttg agggcgtaaa atgcctttgt ataaatcttc
     6901 ggtaatcgca cttgaacaaa tcagtaattg agcggaaagg gtactcatta ccgcagcaag
     6961 aatggcagat aataaaatgc cggctaccca agggttaaat aacaatttag ctaattcaat
     7021 gaaaatttgt tccggatttt tgctgatatt gccgacttgc tccgggttgc caaagaaata
     7081 tgccatacca aaataaccaa tgcctattgc ccccagtaag cagataatca tccaagtcat
     7141 actgatacgg cgtgcatttt caagagactt cacactttct gccgccataa aacgcaccac
     7201 aatatgcggt tgtccgaaat aacccagccc ccacgccatt aagctgataa tgcctaatac
     7261 gctggtgccg gtgaaaatgt cgttgtagtc tttttgtgcc atctcgcctg cacggtcgat
     7321 taaggcttgc atttcatcaa aaccacctaa gttatacatc acaaaaaacg gggtgagaat
     7381 cagggcaaaa accattaatg aagcttgaat agtatccgtc caacttactg ctaagaaacc
     7441 accaataaag gtgtaaagaa tggtagcaac agccccatac caaattgctt ggtggtaagg
     7501 taagccaaat aagttttcaa ataccctcgc accggccaca acgcctgatg cacagtaaat
     7561 tgcaaaaaag agtaaaaaaa tgaccgctga taaaatctta attaaacggg ttttatcgcc
     7621 aaaacggtta tgaaaatatt cgggtaaggt taaggcatcg ccactgtgtt ctgtatgcga
     7681 gcgtaatctg cctgcgacta atttccagtt tagataagca ccgatagtta aaccgattgc
     7741 gatccagcct tcgatcaaac cgcctgcata aacggctccg ggtaatccca tcagcaacca
     7801 gccggacata tctgatgcac cggcagaaag tgcggtcaca aagctaccta aacggcgacc
     7861 gcctaaaata tagtctgaaa ggttattggt aacacgatag gcaataaagc cgatggcaat
     7921 cattcctaga atatagacaa caaacataat tgttgttgga tcaagaccaa acattattaa
     7981 tgaatcctca agatttctta ttgtataaag attaaagtga gtaaagggta aaagattacc
     8041 tgaaataatc aaaatggcaa cacaaaatta ccgtccgccc agctcagctg aaattttttc
     8101 tgctgtttct tgaatagccg gtagtagatt ttcccgcccc agctgattta aacgagcagt
     8161 tgaaagcgaa acagaaatcg cataatggac tttttgttgg tgatcaaaaa tcgggcaagc
     8221 aatacaagaa acccctaatt cattttcttc gttatccatt gcaaaacctt gacggcgaat
     8281 ggtttccagc tcggtttgca ttccgtcaat cgtggtgatg gtattgaccg tcagtttttg
     8341 aatgatgtcc gatttagtct gccaatattg tggaatgtaa tctgccgaac cataagcaag
     8401 aaacagtttt cccattcccg agcaatagag ttgcatttgt tgcccgatat aggctcgggt
     8461 acgcatcatt ccggtggtag cttctaattt attgattaaa atagcgtgat cgttttcccg
     8521 catcgcaaaa tttaccgtct cgcctaaggt taaatttagg gcttctaaat gtggggcggc
     8581 aatatgcaaa atgttaatcg aacttaaggc tcgctgcccg attgccaaac atttggtggt
     8641 taaacgataa ctgcccgcag tcggtgcagg gcggacgtag ccctcttgtt gtaagccttg
     8701 tagcagacgg tgaaccgtgc ttttgttcag ccccgaaagc tcggctaaat gggcaagcgg
     8761 acagccgttt gggaatttcg ctaacaattc caatagtgtt aagccacgaa ttaaactttg
     8821 atttccactc gattcctttt ctttctttgc attcatttgg ctgctcccaa aataatatga
     8881 tctaacggca tatcccaact ctcaatcggt aattgttcga cttgctgaca ttcgtgtgcc
     8941 aaaccgacac taatcagatg aggtgcttga gcaagggttc tatcataaaa gccacctccc
     9001 attcccagcc gatttccggc tttatcacaa gcaacaagtg gggtaaaaat catctctaat
     9061 tcttgcaacg gaaggatatt tctaacatct aatatcggct cttgaatccc caagcggtta
     9121 aattttagaa tagttttgca attgtaattt acaaacaata attgaccgct tgtaaaaggg
     9181 tgcaacagcg gcagatacag ctttttgccc tgttgaagta attgttccat taaaggaata
     9241 ggagaaattt caccgttaaa aggcaaataa aaggcaatat gtgaggcttg gtaagattca
     9301 atcaatgaaa gagcttgagg aataatggat tcggctgctt gagtttgctg accggcagtt
     9361 aaactttgcc gtttaagccg cattgattgc cgtaattggc tacgaagatc ggttaaagat
     9421 gattgattca tataaagacc cgagatgccg ctaccagtaa tggtccttga accagtcggt
     9481 tcaagggagt caaacttaac tgcttttagg cttcttactc ctaattaaat tagggtaagc
     9541 ttgctcacca gcgttagatg tccaactcaa agtttcaaat tatcggctca gggacgtaac
     9601 tcgtaacgca caccccaggc aagacttata gtaacggaat ttgatgattt ttcctagtat
     9661 ttaatcccac aaaatgcaaa ttctcaatct ttttggctct attcctaatc aattcgatta
     9721 aaaattacct aattggattg aaatttgcca agcgaattat ctaactgctc aatacaagcc
     9781 accaacacat ttttattttc tgcattttgg cgttgggctt gctccagttc ataatttaaa
     9841 ttcagtgcca caatcgctaa cactttttca agctgaataa tgccggactg ctctttcaat
     9901 accaccacac gctcttccaa acgctgtgct gaagccagta aatgctcttg ctgttcaaca
     9961 gggcattgca agcgtaaaac ctgcccaaaa atctgtagtt cgatattatt tgccgacata
    10021 aatacgaaat cctcacttaa tcgcaaaatt tttgctatat tatgccattc tttagcaaaa
    10081 tcacaaataa tcccattatg gctatcacag attcccaaca atttaaacaa ctggtatcac
    10141 aattacaaat tgatgataca ccggcagagt ttcacggttt tttatgcggt ttaattgccg
    10201 gcggtattca agatgagtct tggaaaaccc tcacctacca atttaccaat gacggacacg
    10261 cattttccat cgagccgtta aacactttaa ccgattttta tcagcaatta agtgaaagct
    10321 tttcagaagc caacacgctg tttagccttt ggttgccaca aaacgaagaa gatggttttg
    10381 ccttagctga tggtatctcc gaatggacaa acagtttctt acttggactt ggcgttgctc
    10441 aacctaaatt acaacaagaa accgatgagg ttggcgaggc aatcgatgat ttagatgaaa
    10501 tcgccaaact aggctacagt gacgatgata ataacgagga actactggag gctggcgaag
    10561 aagtgttgga atatctgcga gttttagcct tattcttgca cagccatttt gctttaggcg
    10621 tgccgaaaac cgaagaaaag ccaccattac actaatgaat aaatacaagc ggttcaattt
    10681 catctctatt ttgcaaatat attatgcggg gcgaaaaata tttcgcccct accaaaatca
    10741 aaggaaaaac caatgtcaga attaaaaaac gatcgctatt taaaagcgtt attgcgtgaa
    10801 cctgtggata tgaccccggt ctggatgatg cgtcaagccg ggcgttattt gccggaatat
    10861 aaagccaccc gagcagaagc cggcgatttt atgtcactct gccgtaacgc tgatttagcg
    10921 tgtgaagtca ccttacaacc gctacgccgt tatgaactcg atgcagctat cctattttcc
    10981 gatattttaa ctgtgcctga tgcaatggga ttaggtttaa gttttggtgc aggtgaaggc
    11041 cctaaatttg cccgcccggt ggcaagcctc aatgatgtca atcagctacc gattcctgat
    11101 ccggaagacg agttgcaata tgtaatgaat gcggtgcgta ccattcgccg agaattgaaa
    11161 ggcgaagtgc cactaattgg tttttctggc agcccttgga cactcgccac ctatatggtg
    11221 gaaggtggtt caagcaaagc atttaccaaa atcaaaaaaa tgctttacgc tgagcctcac
    11281 attctacaca aattgctgga caagttagct gacagtgtca tcttatactt aaatgcccaa
    11341 atcaaagccg gtgctcaagc ggtgatggta ttcgacactt ggggcggtgt attggctcac
    11401 gaggattaca aagccttctc attacactat atgcacaaaa ttgtggacgg cttaattcga
    11461 gaaaatgaag gacgtaaagt gcctgttacc ctattcacca aaggcggagg cttatggtta
    11521 gaagcgattg cagaaacggg ctgcgatgcc gttgggctag attggacagt cgatattgcc
    11581 gatgctcgcc gccgtattgg cgacaaagtt gcgttgcaag gcaatatgga tccaagcgtg
    11641 ctgtatgcaa gccctgaacg aatcgaacaa gaagtaaaaa acattcttgc cggcttcggc
    11701 aacggcagcg gacacgtctt taatcttgga cacggcattc atttagatgt gccggtggaa
    11761 agcccgaaag tgtttgttga tgcgattcac aactactcga agcagtatca taaataatgg
    11821 ataaaatgcc ctctctcctt gtgggagagg gacagatttt tctttgttca gaagaaaaat
    11881 cagggagagg ggcaagcggt catttttata cagaattttg caaaatcgtt cccttctctc
    11941 tgaaaaataa agaaggaaat acaatgagaa acccgattca taaacgcatg gaacgtttcg
    12001 agccttggca aaacaccaca tttatggcgt gcctatgcga aagaatgtac cccaactatc
    12061 agcttttttg tgaagtaacc gaacagcctg aaaaagctaa ggtattccaa aatattttaa
    12121 atttagtctg ggaatattta accataaaag gggcaaaaat taatttcgat aaccaactgg
    12181 aaaaattaga agaaatcatt cctgatgtga acgaatatgc gttttttggt gtcttacctg
    12241 cacaggaagc ttgcgaagcc ctttctgaac ttcttcacag tattatcgct ggttcaaccc
    12301 ttgaacaagc aatccgcatc agccaaattt cattaggcac agttgccagc tatttggaaa
    12361 tgcaacaaga taaagaactg aacgagcaag agctaaagaa ttctgaagaa attcaagaag
    12421 aactggacgt acagtggcaa atataccgct tgctaaacga atgtgaaaaa cgtgatcttg
    12481 atctcatttt tgaacttaaa gatgaaatca gaagcagcgg gattagtaat atcgggttaa
    12541 atattaacca atagcattga tatttgcaaa atcttgctta ataatacttt gagaaatctt
    12601 aactttgaat taagctatcg caaggtttca ataacttctc agacgtttta cgtcatttat
    12661 taaaaacaca tatcttagag gataaaacta tgaacaaaac tgagttaatc gatgctatcg
    12721 ccgctggtgc aaacttaacc aagaaagatg ctaaagcagc attagaagca actcttgatg
    12781 caatctctgc aagcttaaaa gcaggcgacg ctgttcaatt aatcggcttc ggtactttta
    12841 aagtaaatga gcgtaaagca cgtactggtc gtaacccaaa aactggtgaa gagatcaaaa
    12901 ttgcagcagc taaagtacct gcattcgttt caggtaaagc attaaaagat ttagtaaaat
    12961 aattctttaa agatataaaa ataaagcctg gattataatc caggctttat ttttagcgtg
    13021 tttcgatagc tacagagaaa ctaaaaagca ctatttgctt tcaatcgctt ttttcatttc
    13081 ttcaactttt gtttctgtct caactttcac ttctgctgcc ttctcagcta ctgccttttt
    13141 agcttcttct gctttatctg ctgttgcttg agtcgcatca cttgctgctg cttttagttc
    13201 ttccacttta gcttcagttg tggcttttaa ctcagcagct ttctctgcta ctgccttttt
    13261 tgcctcttct gttttatctg ttgttgcttg agctgcatca tttgctgctg cttttagttc
    13321 ttccacttta gcttcagttg tggtttttaa ctcagtggct ttctctgcca ctgccttttt
    13381 tgcctcttct gttttatctg ctgttgcttg agctgcatca tttgctgccg ctttcaactc
    13441 ttcaaccttc gcttcagtag ccgatttaac ttctgcagct ttttcagcta cagcttcttt
    13501 tacttcaacc gctttgtctg tagttgattg cgcggcttct tttgcctgat cacaagcagc
    13561 aagtgccata gttgtaccta acactaataa tgataaaaat gattttttca taccgagttc
    13621 cttaaataaa ctaaaaataa cgcactttgt aataaagtgc gtttacttag gcagaaaaat
    13681 tgatagaaag ttcatacttt gataaaaaaa tgcctattag ttataactaa taggcattta
    13741 aaccaattaa ttgtacttaa ccattggttg aacgaggcgc tgactgcact tggctcactg
    13801 cgctggtata tccgccaaac cctttgccat agaaatagta tttagagttt tgccattggc
    13861 taatttcgac tacttcaagc ttgtcacttg gtgcagaagc aatggtcata gcggcttggg
    13921 tatgctcaac acgagagaat gtacctaatc tgccgttaaa tcgataatcg ctttcgtaag
    13981 ttgattttgg cacttcaatc gggttagtaa cctcttccac aatcgctggt gcaaccgttg
    14041 cttgcgattg gaatttactg actcgctcat ttttctcttt aactaaatca cgttcagaat
    14101 gctgagcaac aacactacca aatggagcaa tatgctcttt tacctctact ggttttacgt
    14161 cttcagaagc aactttttct tctttcggtt tggaaagctg ttcttgcttc tccttaacca
    14221 aatcacggtt agttgcttgt gctacaaagc cacctagcgg actaactgta tgaagcttct
    14281 tctcttctga ttttttctct gtctcaacaa gctgattttc ttgctgttca agcatttgat
    14341 caacagataa gaatgatgct ttttcctcta ctttgacagt agaggtaacc tgaacccaaa
    14401 ctttaccgct cgcaagctca ggcgaagcaa cagctgaagt tagaggcata ggagaaataa
    14461 cctgattgcg ttgattttca cgacgacgct gattgcccac acgcaaatgg cgaggtaaac
    14521 ggcgttggcg gttattttca cgtccttcgt tcgttgtttc tacctcagga gctgtttcaa
    14581 caggtacttc ttgggttgca agcggttgaa tttgttgatt tttttgcaca ttttcaactt
    14641 gttttgccac cgtttcctca acagcaggtg ttagattttc cacttctact tcttgtgttt
    14701 cgatacgcac ttttttacgt agatcacggc gttggcgacg ttcagtttga acctcacggg
    14761 agttgctacg actctcttgc tcggttacac cggaaacttc ttccactacc gctcggcgtg
    14821 cttttttctc gctacgcatc ggtttttctt ccgctggagt agtttgagct tgcggagttt
    14881 tacgctcttg attttccgct ttagcattac ggttcgggcg ttcacggcgt tggcgacgct
    14941 cacgctgatt gcgtgctttc ggcttcggtt tttcttccac tttttcttca ctaaaccagc
    15001 ctttaatggt cgcaattaag cggcttaaca gcgatggttt tggtggttcg gttggtgttg
    15061 gcgaaggttg gagattcagc tccgagcttt gtaacactga ctctaccgta atcaccggct
    15121 cgttacgggt aaccagcaca tcttcttgat ggtgatgatg tgggtgttct tcttcatgac
    15181 gagcttcgta atgcttggtt aaatcgtagc ttaacgttgg agtaatttca ttttcacgca
    15241 ggcgatatac gctgaaatgc ggcgtttcca tactttcaga cggtactaca acaatttgta
    15301 catcgtggcg tttttcaatg ctggaaatcg ctttacgttt ttcgttgagt aagtagctgg
    15361 cgatttgcac cggcacaatg gtgtgaacct gtgcagtgtt ttctttgatt gcctcttctt
    15421 ctaataaacg taagattgac agtgcgattg attcattatc acgtacttta ccagttccct
    15481 gacagcgagg gcagatatgc gatgaagcct cacttaatga tgggcttaaa cgctgacggc
    15541 tcatttctaa caaaccgaaa cgggaaatac gggcaaattg gattctcgca cggtcttggc
    15601 gtgtcgcctc acgaatacgg ttttctactt cacgttggtg gcgaaccggt gtcatatcga
    15661 taaagtcgat cacaatcaaa ccacctaagt cacgcaaacg taactgacgg gcaatttcat
    15721 ctgccgcttc taagttggtg ttaagagcgg tttcttcaat atcgccgcca cgggttgaac
    15781 gtgaggagtt aatatcaatt gctgttaagg cttctgttac atcaataacg atagagccac
    15841 cggaaggtaa acgcacttca cgttggaatg ccgattcgat ttgcgattca atttgatagt
    15901 ggctgaatag tggtacttcg ccttcgtata atttcacacg gttgatgaaa tccgggcgaa
    15961 caaggcgaat gtggtttttg gctttctcga acactttttt gttgtcgatt aagatttcgc
    16021 caatatcacg gcgtagatag tcacgaattg cacgcacaat cacgtcactt tcttggtgaa
    16081 ttaagaacgg agctgggcga gattccgccg cttttttaat cgcttcccag tggtgtaaca
    16141 agacttttaa gtcccattgt aactcttccg gcgatttgcc gacaccggcg gtacgaacga
    16201 ttaagcccac gtcttccggt acatctaagc agtctaaagc ctctttgagt tctaaacgct
    16261 catcaccttc aatacggcgg gaaataccac cggcacgagg gttgttcggc attaagacta
    16321 agtaactgcc cgcaagcgaa ataaaggtgg ttaaggctgc tcctttgtta ccacgctctt
    16381 ctttgctgac ttgaacaatc acttcttgcc cttcttgaat aatatctttg atattcgggc
    16441 gaccgttaaa cacatagtct gacgggaagt attctcggga aatttctttt aaaggtagga
    16501 agccgtggcg ttctgcaccg taatctacga atgcagcttc aaggcttggt tcgacacggg
    16561 taattttacc cttgtagata ttggattttt tctgttcgtg acccgggctt tcaatgtcca
    16621 agtcaaataa gcgttgccca tcaacgaggg caacacgcaa ctcttctttt tgagttgcat
    16681 tgattaacat tcttttcatt gtgttttctc attattttgt ttttgtctta cacggtcatt
    16741 ttgacccatt aataagggaa tggatttaat gtctatctca cgactgtctt cattcccgat
    16801 agtgcattga tggttgtctt acgtttactt tgtcttgtct taaagaaact gcaaaatcaa
    16861 tcacttaagg cttgtttatc tctattacaa gcggttattt ttgctttatt ttttgcaatt
    16921 ttctattgct ttttcaagcc aaaaataacg agtcattatc gcattttaac cctgaaaaag
    16981 gcaaggtaat tgttgtatgg tagctgttat ttgttgtgga gatgaatata atgaaaatga
    17041 atggggtgat aaggaaaaat aatgagattt agtgtgagtg ctggagataa gcgtgaaatc
    17101 caagcggtta aatttgcaaa aaaagagcta aatttaaccg cttgtaaagc gaatattatg
    17161 cttttgccgt catttttttc actgcttctt cagtggtcca gaggtcattt gcgataatac
    17221 ggtagttggt taatgctgca aggtagccgt caccttcagg aatacgaggc cctgcggttg
    17281 catctttcac cactgttact tcaaaacctt gttcgattaa ttcacgcaag tgtgaatcaa
    17341 tacataagtt ggcagccata ccggctaaaa ttacttggtc gattttacgt ttgcgtaatt
    17401 gcaacactaa gtcattggtt tccggtccaa agattttgtg tggagaggta atcactgttt
    17461 tgccatcaaa aatgtatggt ttgtactcag gcataaagtc tgcacctgaa ttttcaaagc
    17521 cttccatggt gtattgacct tttctcgcaa acattccgct gtcgtgcata aagtgttcac
    17581 ccggtgaacc aaattcccat ttgtgatcag tcgggtaata gtagtgtggc gaaacaaagg
    17641 ttggcatatc gactgctttt gctgctttaa ataagctttc aatgttagct acagtgttgt
    17701 tttctttaat tgagtcacct aacacgcccc acatcacgcc tttcgggcct aggaagtcaa
    17761 tttgtggatc aactaccact aatgccacac gttttggatc aatggtcatt ccatggcgtt
    17821 ttaaaccgta ttgttccggc tcggcataag gtgatttttc tgcactggct tgttttgcat
    17881 ttgctgttga tgcaatacct aatgcaccta atacactggc tgccattgca atacgagagc
    17941 cgtggttaag ggcttgacgg cgttcttggt tgatttcttc aaattgttcc tgtgacatca
    18001 ttttatcctc attaatagat atacaaattg taatgataac ttgcgttacc gaacgggttg
    18061 ctattatagt gatcgcattt ctcctgtttt aaccgttcgt ctcaattctt tacccaagga
    18121 gcttaatgtg gatagtttga ctcatctttt tacccaattt cagtttcgca cggatttatt
    18181 tcatctaggt cagttatgcc aaatcgggag ttttaatgaa gagaataaag gctatttaca
    18241 tttcgttcgt cagggacgtt ttattttaaa tcaggctgcc gaacgcccga ttacgattga
    18301 acgcccagcg attatttttt caccaaccaa tattttgcat agtttgcacc cgttagatga
    18361 tagtgggtta gatatatttt gtattaattt tgattttggc aaagggattc gcaacccatt
    18421 aactcatacc ttgcacaata ttgtgatctt agagttggca cagtatccgg aattgggggt
    18481 attagccgat caaattttta gtgaagtgaa tcagaaaggg tgtggttatc aggcaatgat
    18541 tcatcattta tgtgcctatt ttaccttgaa agttgcccgc caatgtttgg aaaaagggta
    18601 tattcaaaca ggtttgttaa aaggcttggc agataagcaa ctggggaaag tgctgttagc
    18661 tattcacaat gcaccagagc gagattggaa tgtagaggag ttggcaaatg tggcattaat
    18721 gtctcgctca cgctttgctg cttattttaa acaaacgatg ggggtttcac cgatggatta
    18781 tctcactaac tggcgactag cggttgccca gcatttatta caaaaaggga taccagtagc
    18841 attggtggca gagcaagtcg gttatagcca taatgctgct ctcagccgtg tctttatgcg
    18901 ggaattaggg ctttcgccta ccgagtggct ggctcaaaat aaagagtcgt aacctgatga
    18961 gaaacaaata gccgaatggt aaaaatgtca aaaaatttaa ccgcttgtta tgttgcacaa
    19021 gcggttaaat gttgccttaa atgccaagag aatcccaaat ctcatcaatg cgtttcacca
    19081 cattgtcatc tttttcgatg ggtgttcccc attctcgggt ggtttcaccc gcccatttat
    19141 tggtggcatc aatccccatt tttgagccta accctgcaat tggcgaggca aaatcgagat
    19201 aatctatcgg cgtgtgttca atcagtgtcg tatctcgagc cgggtcacaa cgggtggtga
    19261 tcgcccaaat tacatctttc caatctcgag cgttcacatc gtcatcgcac acaatcacaa
    19321 acttggtgta cataaattga cgcaggaacg accaaactcc catcatcacc cgtttagcgt
    19381 gtccggcata ttgttttttc atcgtcacta ccgctaaacg gtaagaacag ccttcaggtg
    19441 gcaagtagaa atcgacaatt tccggaaact gtttttgcaa aatcggaata aagacttcat
    19501 tcaatgcttc acctaatact gccggttcat caggtggtct gccagtgtag gtagaatgat
    19561 aaatcgcatc ttttcgcatc gtaatatggg taacggtaaa taccggaaaa tactcttgct
    19621 cgttgtagta gccggtgtgg tcgccataag ggccttccaa tgcggtttct tccagatcga
    19681 tatagccctc cagcacaatt tcgctacttg ccggcacttc taactcattg gaaatagatt
    19741 tgaccacttc ggttttatgc cctcgcaaca gccctgcgaa cgcatattcc gaaagcgtat
    19801 ccggcacagg ggtgactgcc gctaaaatag tggctggatc tgcaccaatc gcaaccgaca
    19861 ccagaaacgg tttacccggg ttttcggctt tccactcttg gaagtctaac gcaccgccac
    19921 ggtgggaaag ccaacgcata atcagtttgt ttttgccgat aagttgctga cgataaatgc
    19981 ctaaattctg gcgttttttg taaggccctt gcgtaatcgt cagcccccaa gtgaccagtg
    20041 gagcgacatc gcccggccaa cagtgcataa tcggtaattt gtagagatca acctcatcac
    20101 ccgaaatcac cacttgctga caatcggctt tgctcagtac cttaggcggc atatttaaaa
    20161 tttgcttcca ttgtggcagt tgcccgatca aatctttaaa ccctttcggt ggttcaggct
    20221 cttttaaaaa ggcaagtaac ttgcccagct cacgcagagc tttggtatct tcttgcccca
    20281 ttcccagagc tacccgtttg ggcgtgccga ataaattaca caacaccgac atttgatagc
    20341 ctttcgggtt ttcaaacagt aatgccggcc cgcctttgcg taaggtgcgg tcggcgattt
    20401 ctgtcatttc taaataagga tcgatttctt gctgaatacg gactaattca ccctgagact
    20461 caaggaaatc gagaaattca cggagatttt tatacttcat agcggtcata tttttcgatt
    20521 tttttgcaag tatatagaaa aacgtaaatg aaaaataccc ataaaaaatt tatagaaatt
    20581 ttgttttatt ttgttagaat agctcggttc aaattaaatg ccataagggg aattttatgt
    20641 ttaaaagaac attaatcggt ctagccgtgt taggtgcaag tgttgctcaa gcggatgtat
    20701 taaccagtat taagccttta ggctttattg ccagtgccat taccgaaggg gtaactgaaa
    20761 ctaaagtatt attaccggta actgcctctc cacacgatta cagcttaaaa ccttcagatg
    20821 tgactcaatt acaatctgct caattagtgg tttgggtggg tgacggcttg gaaggctttt
    20881 tagaaaaaag tattgaaaaa ctgccgagag aaaaagtatt aacccttgaa gatgttgatg
    20941 cgattgatga tattgtcgat aaagcaagca aaaaagaaga taaagacgat gatcacgatc
    21001 ataaacatga acataagcac gaacacaagc acgagcataa acacgaaggg cataatcacg
    21061 aaggacataa ccacgatcat cacggacata gccacgatga agattggcat atttggctct
    21121 caagcgaggc aagtgagctg gcagcagagc aaattgcggc acgtttaagc gaacaaatgc
    21181 ctgaacacaa agcgaaaatt gcggaaaatc ttgcaaaatt taaagcaaat ctaaccgcta
    21241 aaaaagccga aattgcgaaa caattagagc cggtaaaagc caaaggttac tacacattcc
    21301 acgatgctta cggctatttt gaaagagaat atggtttaaa ttcattgggt tcatttacca
    21361 ttaacccaac tgttgccccg ggtgcaaaaa cacttacagc aatcaagaaa aacattgcga
    21421 aaaacaaagc acaatgttta tttgccgagc cgcaatttac gccgaaagta attgaaacct
    21481 tagcaaaaga aactaaagta aatgttggcc aattagaccc actcggtgca aaagttcagt
    21541 taagtgcgac tgcttaccct gaatttttac aagttttggc tgacgaattt agccaatgtt
    21601 taacaaaata agttaaaaat cccccattta aatataaatg ggggattttg cgattacatc
    21661 acttaagctt tttttaagaa ttctgatttc aacataattt taccgcaatc gacattatgg
    21721 tcaccttgag ctaaacggat tcctttgaat tttgtacctt ttttcagtac ttcagacgag
    21781 ccttttaatt ttaaatcttt gattaataat acatcgtccc cgtcagataa taaattgcca
    21841 ttactgtctt ttacaattag cacatcatca tcttgatccg tttcttcgcc cgtccattca
    21901 tacgcacaat ccgggcaaac aaattgaatt gagtcgtggt aagtattttc gcttttacat
    21961 tcagggcagg ttgggtaaat cattttaatt cctcaaatta ataaagaacc agattatata
    22021 ccgaaatcca gcaaaacgct aagcggtcat attttgccga ttttttacca atcgagctaa
    22081 aagtcagtta gaatagcctt aattacacaa aaaggaggat actatgatag tcgatccgaa
    22141 ttatcagcaa gaattagaaa aatacgaaaa tccggtacca agccgagagt ttattttaaa
    22201 tacaattcgt gaacataacg ccccaatgag ccgtgatgaa ttattgtcgg cttttcatat
    22261 tgacgatgaa gaacgaatgg aaggcattcg ccgccgtctg cgtgcaatgg aaaatgacgg
    22321 tgaattggtg ttcaccaaag gcaaacgcta tgcgttgccg gaaaaaatgg atttaatcaa
    22381 aggtacagtt atcggccatc gtgacggtta cggttttttg caagtggaag gacacgaaaa
    22441 aggggcgact aaaagcgaag attggtttat tcccaacgcc caaatggtaa aggtaatgca
    22501 cggtgacttt gtgctggcac aaccaaacgg cacagaccgc agaggtcgga aagaagtccg
    22561 cattgtgcga gtgttggagg cccgtaagaa acagattgtc gggcgttttt tccttgaaag
    22621 cggtatcggt tttgttgtgc ctgatgacag ccgcgttaac caagatattc tcattccgga
    22681 tgaacaccgt atgggggcga gaatggggca agttgtggtg gtggaattac aggaaagaaa
    22741 agcgagtttt agccgcccgg tgggaatcat tactgaaatt ctaggcgata atttagcgcc
    22801 gggaatggaa attgaaattg ccctacgcaa tcacgatatt ccgcacattt ggccggaagg
    22861 ggtagaaaaa caaatccgcc aatttagtag cgaagaagtg ccggaagaag ccaagcaagg
    22921 gcgggtggat ttgcgagatc tgccactgat taccattgac ggcgaagatg cccgagattt
    22981 cgatgatgct gtatttgcca aaaaagaggg caacggctgg cggttatggg tggcgattgc
    23041 cgatgtaagt tattatgttc gcccaaaaac cgcattagat ttggaagccc taaatcgtgg
    23101 taactcggtc tatttcccga atcgggttgt gccaatgttg ccggaagtgc tttccaatgg
    23161 cttgtgttca ctcaatccac aggttgacag gctctgcttg gtggcggaaa tgtcggtttc
    23221 cagcaaaggg gaattacaag attatcaatt ctacgaagcg gtaatgaact ctcacgcccg
    23281 tttaacttac actaaagtgt ggaaaatgtt ggaaggtgat gaagccttgc gtgaacgcta
    23341 tgctccatta gtgccgcata ttgaagaact ctatgcaatg ttccaaacct taatgaaagc
    23401 tcgccataaa cgaggggcga ttgaatttga aaccattgaa aatcaattta ttttcaatcc
    23461 gcaagggcga attgagcgaa ttgagccgtt aatccgcaac gatgctcata aattgattga
    23521 agaatgtatg attttagcca atattgctgc cgctcgtttt gtggaaaaag cgaacgaacc
    23581 ggcgttatat cggattcacg ataagccgag tgaagaaaaa ttgctcagtt ttaaatcctt
    23641 tgtgcgtgag tgtggcttaa cctgggacgt aggcttagat ccaaccccaa aagattacgg
    23701 tgtactactg gagcaaattg ccgaacgtgc cgataaagag ttgattcaaa cgatgttgct
    23761 ccgctcgctg aaacaagcag tgtatgcggc ggataatatc gggcatttcg gcttggcatt
    23821 aaccgaatat gctcatttta cctcgccgat tcgccgttac ccggatttac tgttacaccg
    23881 tgcaattaaa tatctgatcg aaaaaggcaa aggcaacact cgctactata ccgatggcgg
    23941 tggttatcac tacaagttag atgatatgga cgaattcggc gataaatgtt ctgccaccga
    24001 acgccgagct gatgaggcaa cccgagaagt ggcagattgg ctgaaatgcg aatttatgca
    24061 agatcatctt ggcgaagagt ttgatggcgt gatttcgagt gttaccggct ttggcttatt
    24121 tgtaaaactc aatgagcttt taattgacgg attggttcat atttcaacgc tggataatga
    24181 ttattaccac tatgatgcgg accgacaacg tttaatcggt ggcagtggga tcatctatcg
    24241 tttaggcgat gcagttagag taaaagtaat caatgtgaat ttagacgaga aaaaaattga
    24301 ttttgagtta ctcaccggta ataagaaaac cggcaaaacc gccaagaaaa aagcaaaagc
    24361 gacagaggtt cctgttttca aagagcctaa aacggtaaaa aaagccaaaa aagtcgctga
    24421 gaaaaaaccg gcaaaatctg caaaaaaacg gacaaaaacg accgctagta aatcaaggaa
    24481 aaaatagcaa atggattaca aaaaacaaat tttattcagc cttttatttg ctttagtaat
    24541 tgcaataata agctattttt tgtttaccta tatcccgttt gattgtagcg atctgagcaa
    24601 actcaacgat tcacaacgtt tagagtgccg aactgccgaa tttctgggcg tttattactg
    24661 gctgccgcac actaccacgc tgtttttcgg ctcggtgtta ttatattggg cggtttatgg
    24721 catgatttca ttagtgcaag cagtgctcaa acgctaacaa gcagttttgt ttttgcaaaa
    24781 agttgcaaat ctcatctgaa taatccttcc aagatcaaag gtctatcaaa aaagatagac
    24841 ctttattgtg taaataaact aaaactcctt gatagatgta tcacatatct ataaaattcc
    24901 agcaattaag tgggaaaaag tggtaaattg tggaactttt ccttaaattt aacctcattt
    24961 aaaaaggtaa atcagtaaaa tgtttcgtgg tgcaagttca atcagcattg atagcaaagg
    25021 gcggatcgct attccgaccc gttatcgtgc cgagctattg gaaaaacatc acgggatctt
    25081 agtttgcact gtggatatcc gccagccttg tttgttgctt tacccacttc acgaatggga
    25141 aatggttgaa cagaaattgt tggcactatc taattttgac cctgttcaac gccgtattca
    25201 gcgagtaatg caaggctttg ctaccgaatg tgaaatggat tcggcagggc gaatactgct
    25261 tagccctaca cttcgccaac acgcacaatt agagcaacaa attatgctgg ttggacaatt
    25321 aaacaaattt gaaatttggc aagataaaca atggcaagca caaattgccg aagatcttgc
    25381 ttttggcagt tcggcagaaa ttttagactg cgaagcattg aaaaacctgt ctctctagta
    25441 attggaaatt gagatgagcg agaacccaaa acatattaca gtattattgc acgaagcggt
    25501 tgacggcttg gcaattaagc ctaacggtat ctatattgac ggtactttcg ggcgtggcgg
    25561 gcattctcgc ttgattttat ccaaactggg cgaaaatggg cgattaatcg caactgatcg
    25621 tgacccacga gcgatagccg aagctcaaac gattagtgac cctcgttttc aaattgaaca
    25681 tacggcattt tctgccattc ccgaactgtg tgaacgaatg gggttggtgg gcaaaattga
    25741 cggtatttta ttggatttag gtgtgtcttc cccgcagctt gacgaagccg aacgaggctt
    25801 tagttttatg cgtgacgggc cgcttgatat gcgaatggat accaccaaag gcttatcggc
    25861 agccgagtgg cttgcccaag tttcggcaga agatttagct tgggtattga aagaatttgg
    25921 cgaagagcgt tttgccaaac ggattgcaca agcggtcgtt tcttacaata aatctgcaaa
    25981 tgaaaaaatc agccgcactt tgcagcttgc gcaaattatt gcggatgcgg tgccgtttaa
    26041 agataagcac aaacaccctg caacccgttc gttccaagca attcgcattt ttatcaatgg
    26101 tgaattagac gagctggaaa aagctttaaa ttccgcatta acggtgttag cccctgaagg
    26161 gcgtttgtcg attatcagct ttcattctct agaagaccga atggtgaaac agtttgtgcg
    26221 taagcatagt aaaggaatgg acgtaccccg tggtttaccg attttagaag cagagctaac
    26281 caaaaatatt ccgctgaaaa ccattggcaa agcgattatg ccgagcgaag ccgaaattga
    26341 agcaaatgcc cgctcacgca gtgcggtatt gcggattgcc gaaaaacgct aacagtaaat
    26401 agggaagaat atgtcgaacg aacgttatcc gttacatcaa atcatcattg atgatatctt
    26461 tagccataac aaagtggctc ttttattgtt gattggtgta gtgatttcag cagtggctac
    26521 gatttggata acccaccaga ctcgtttatc ggtgagcgaa caagggcaac tgattcaagc
    26581 aaaccaaaag ttggaaagcc aatatactaa tctgcaatta gaagaaaaca gcagaagcag
    26641 tcgggcaaga gttgatgccg ccgctaaatc gttcggatta cagccgatta aaaaagagca
    26701 agaaattatt ttagttgaat aaaaaatggt taaatcagtt aaattaaaac gtaagcaaga
    26761 agcccctgtc gcaaaagcag agcctcaaca agcagaaggt aaaaaaacac cgagtttttt
    26821 acctactcgc tttagagtgg ttgtgttttt tatgctatta atggttggtg gcttactagc
    26881 acaaaccgcc tatattcagc tttttgactc tgaaagatta attaaagagg ctaataaccg
    26941 ctcacttcgt actaaagaac tccaatttac ccgtggacgt attttagatc gtaacgggcg
    27001 ttttctctct attagtgtgc caatgtactc attaacactt gacccgagag aatactttga
    27061 tactaagctg cgtcgttcta acgaaagctg gcgtgcctta gcgattgaaa tggaaacatc
    27121 aaaaggtaaa attgaggcaa acgttaataa atttattgaa aagaaaaatt tttcaaaaga
    27181 aaaagtagat tttgatccac gttctatttt aaatactaaa agtgaaggtt attggactca
    27241 gcttgcccaa gttacaggtt tggattacaa cagtttaata gaaaaagtac ggaataaccc
    27301 tagctcggtt tttttacgtt tagataacga aaatttagta agagaacgcc agaaattcca
    27361 agcgttaagc aaagaaatcg gccgttccta taatgacata atggacgagc tttatagcaa
    27421 aaaccgccag cgattccttt acttatcccg tcatcaatct gaagcgatca gcgaatatgc
    27481 aaaagaacta gatattgatg cattagtgat taaagtcgaa tcacgccgtt tttacccgct
    27541 ggcagaagaa tcttcacagt tgatcggttt tactgataaa gatgatatgc acggttcgga
    27601 agggctggag cgtagttttg attccttact tatcggcaaa aacggtaaac aaattatccg
    27661 aaaagatgcc aaaggcaata ttgtggaaaa tatccgttcg gagaaacagt acgatccgca
    27721 agatgttatg ttaagcattg atgaagaatt gcagtctatg gtgtacggtg aaatcaaaaa
    27781 agcggtacag gaaaataatg cggaatcagg cactgcggtt ttggttgatg tgcaaaccgg
    27841 cgaaattttg gcaatggcaa atgccccttc attcaaccca aacaaacggg atagttttaa
    27901 acctgaatta atgcgtaacc gtgctattac cgacactttt gaaccgggtt caacggtgaa
    27961 accttttacc gtattaaccg cattacaaaa tggggtaact tatcgtgatg aagttattgg
    28021 cactcgccca tttgtcgtca atggacatac aattaaagac gttgctccaa gggacagcct
    28081 tacgcttacc ggcatcttgc aaaaatccag taacatcggg gtcagtaaat tagccctcag
    28141 aatgccggca aatgcgttag tggatactta cacgaaagtc ggtttcggta aagacaccgg
    28201 tttaggcttg ggtgaacaac gaggtacgaa cggtgatcgc aaacgttggg cggatattga
    28261 acgtgctacc ctttcctatg gatacggttt aaatgtgacc ccattacaac ttgcccgtgc
    28321 ttatgcgact ttaggaagtt tcgggctgta tcgcccactt tcgattacca aggtcgatcc
    28381 gccggtgatt ggcgaacggg tgctaccgga aaaaaccacc cgagatgtcg tgcatatgat
    28441 ggaaagcgtt gcggctaaag gcgaaggcgg acaccgtgca gcggtggacg gctaccgtgt
    28501 agccatcaaa accggtacag cacgcaaatt agaaaaaggt aaatatgttg aaaaatatat
    28561 tgcttatacc gcagggcttg ccccggcaag cgatcctcgc tttgcattag tggtattaat
    28621 caacgaacct aaagcaggca actattatgg tggctttgtt tctgcaccac tgttctcacg
    28681 cattatgggt tacaccttaa aacaacgtaa tattaagccg gataacttaa cggaaaatac
    28741 ccaaagtgcc gtgcgtgaaa ttaaattaga acgccctatc aattaaggtt agctatgaaa
    28801 cgtttactcc cttttctgac agaacttgat gcttgggtgg aagaactcac cgagctaaaa
    28861 caaatgacac ttgatagtcg ccaagttcaa gccggtgatc tctttgttgc tttaaaagga
    28921 catcaagtcg atggcagaaa atttattccg aatgcgatag aacaaggagc agcaattatt
    28981 ttggctgagg ctgatgaaga tcaaccggaa attgagcttg atccgaaatt tgcaaaattt
    29041 aacctagatc gcaccgcttg ttgcaaggtt gtctctgttc cacgtctgcc aaaattgctc
    29101 tctgaaattg caggagctct ttaccataac ccgtcacaca agctgacttt agccggcatt
    29161 accggcacga acggtaaaac gactactgct caattattag cacaatggca taatttattg
    29221 ggtgggaaat cggcagtaat gggtactatc ggcaacggtt tgtacggaca agtgcaagaa
    29281 gcggtaaata ccaccggctc tgccattgaa attcagcata atcttgcgaa ctttgttgaa
    29341 cttggagcgg atttttgtgc gatggaagtc agctctcacg gtttggctca atttagagcc
    29401 gaagccttgg attttgattt agcgatcttt acgaatttaa gccgagatca ccttgattac
    29461 cacaacacga tggaagagta tgcccaagcc aaattccgtc tcttcaacga attaagcacc
    29521 aaagctcaag taattaacgc cgatgatgaa atcgggcgtg aatggctgac acaattacca
    29581 aatgcagttg cggtaagcac cgatccgaag tttgcaggca accaccaatt tgtcaaagcc
    29641 actgcggtaa aatttaccct acaaggggca agtattgcgt ttgaatcaag ttggggcaat
    29701 ggcgagttac atagccgttt aatcggggct ttcaatgtaa ataatctgct taccgccttt
    29761 gccggacttt tagctcttgg ctttgatatt caagaactgg ttaaaaccgc cccgaattta
    29821 gttggcgtag caggtcgaat ggaatgcatt accgctccaa ataagccaat ggtaattgtg
    29881 gactatgctc acacccctga tgctcttgaa aaagccctac aagcggctcg tttacattgc
    29941 gaaggcgaac tttggtgcat tttcggctgt ggtggagacc gtgacgccgg caaacgccca
    30001 ttaatggcga cgattgccga gaaattaacc gataaagtga ttgctacaga cgacaaccct
    30061 cgcaccgaag ataatcaaaa gattatggca gatattgtga aaggttttag taaaccccaa
    30121 caaattatcc acaatcgtga agaggcaatt aaaaccgcca ttgagcaagc taaagccaca
    30181 gatgtgattc taatcgcagg caaaggtcac gaagattatc agattattgg cacagagaag
    30241 ttacattttt cggatcagga aacggcaagg aaatatctgt tttaatgtca ctttctttgc
    30301 acaagcaaag aaaagtgaca gtgacaaata caaaatgctc gtaattaatt ttataaagaa
    30361 aaagataaat caatgataaa actaaccacc caacaaatcg ctgaaatcct aaatgccaaa
    30421 ttgatcggca atggcgaaac tgtggtagaa accaccagca ccgacacccg acaagcggtt
    30481 gaaaacggct tattttttgc attaaagggc gagaactttg atgctcatca ctatcttcaa
    30541 aatgcagtcg cacaaggttg tgtagcggtt gtggttgagc gtgagtgtga aattgatgta
    30601 ccacaacttg tggtggcaga tacccgtttg gcattggggg aattggcgaa atggttaaaa
    30661 gctaagctga acccgaaaac ggtagcgatg acaggttctt ccggcaaaac aaccgtcaaa
    30721 gagatgacgg caaaaattct gcaaaaaatg accgcttgcg aacaggaagt gctttatact
    30781 tttggtaact taaacaatga cttaggtgta ccgatgacct tgcttcgctt aaccgaacag
    30841 cataaatatg cggtcattga gctgggggca aatcatatcg gtgaaatcgc ttataccacc
    30901 gctatcgctc agcctgatgc ctgcttggtg aataatgtgg ctgcggcgca tttagagggg
    30961 tttggttcat tagcaggcgt ggcacaagcc aagggcgaaa tttatcgtgg cttgaaagaa
    31021 aatggcaaag cgattgtcaa tttagctttt tattatccgc aatggcaaaa agagattggc
    31081 tcacgggaaa tgcaatcttt ctcttatatc gggtcagatt cggattctta cgcagattac
    31141 tgggcggaag atgtagaact gcatttaagc ggttcacgct ttacgctaca taccccacag
    31201 ggcgaaattg ctattgatct gccttatttg ggcaaacata acgtgagcaa tgccttagcg
    31261 gcaacttcgc tggcgatgtc tgttggggca acgctagaag cagttaaggc agggcttgag
    31321 cagcgttcaa tggtaaaagg gcgtttgtat ccggtgcaaa ttaacgacca ctgtttgttt
    31381 attgatgaca cttacaacgc caatgtggat tcaatgaaat cggcgatttc tgttttggaa
    31441 aattatccgg cttatcgtat ttttgcagtg ggtgatatgg cggaattggg tgaagaaagt
    31501 agcaaatgcc accaagaagt agcggatttt gccaaaaatg cagcattaga tttaatcgtg
    31561 agctttggaa aagaaagtgc ggtaatcagc caacaggcag aacatcattt taccgataaa
    31621 cagcaaatgg ttgatttcct actgccgatt attttgcaaa aaatcgaaca aaaacaaccg
    31681 cttgtattat tggcaaaagg ctctcgtagc cagaaaatgg aaaccgtgat ttccgaattg
    31741 tgtaaaccgt ttaacctaaa tttctaatta tttttaagga ctaagcaatg ttagtttggc
    31801 ttgcagaact attggttcaa tataacacag cgtttaacgt tgtctcttat attactttcc
    31861 gtgccattat ggcgttatta actgcaatgg gaatcgggct ttggatcggg ccgaaagtaa
    31921 ttcgccgttt gcagatctta aaatttggtc aggaagtgcg taatgacgga ccggaaagcc
    31981 atttcaaaaa acgtggtacg ccaacaatgg gcggagtgat gattctcgca gcgattgggg
    32041 tgagtacatt gctttgggca gatctgcgta attcctatgt ttggttcacg ctgtttgtgc
    32101 tatttggcta cggtgcagtg ggctttgttg atgattatcg taagatctct cgtaaaaata
    32161 ccgatggttt gattgcccgt tggaaatatt tttggttatc tgctatcgca ttagtggcgg
    32221 tgtttggtat gtatgcggtg ggtaaagata ccgctgcaac acaattagtc gtgccgtttt
    32281 ttaaagatgt gatgccgcaa ctcggtttat tttatattgt gttatcctat ttcgtgattg
    32341 tcggcaccag taatgcggta aacctaacgg acggcttaga cgggcttgcg attgtgccaa
    32401 ctattatggt cgcatctgcc tttgcgttaa ttgcgtgggc aaccggtaac tttaattttg
    32461 cacaatattt acatattcct tttattccaa atgccggtga attggttatt ttatgtactg
    32521 cgattgtggg agcaggttta gggttcttat ggtataacac ctaccctgca caggtgttta
    32581 tgggcgatgt tggctcactt tcgctcggcg gtgcattagg cgtgattgca gtattagttc
    32641 gccaagaatt attattggtg gtaatgggcg gtgtatttgt ggttgaagcc ctctcggtga
    32701 ttttacaagt aggttcatac aaattacgcc aaaaacgtat tttccgaatg gctccaattc
    32761 accaccattt tgaactcaaa ggctggtctg aaccacgggt tatcgtgcgt ttctggatta
    32821 tcacgctaat gctggtttta gtcggcttag taacattgaa attacgttaa ttgattgaat
    32881 aaggtgcatt gagataatgt accttatgtt atttttaaat tacagaataa attatgcaaa
    32941 aacaatacga aaacaaaacc gttactatta tcggcttagg tacaacaggg ttatcttgtg
    33001 tggatttctt taagaaaaaa aatgccaata tccaagtgat tgacacccga gcaaacccag
    33061 ccggagcgga taaattggac gtaactattc cgttgcatac cggtgggtta aacagtgaat
    33121 ggttgctgaa atctgatctt attgtaatta gcccaggttt agccgttgcc acacctgaaa
    33181 ttcagcaagc gattcaagct ggtgttgaaa tagtcggtga tgttgaactg ttttgtcggg
    33241 aagcgaaagc tccgattatt gcgatcaccg gttcaaacgg caaaagtacc gtaacgactt
    33301 taactaccga aatggcgaag caagcgggga ttaaagtggg aatgggtggc aacatcggtg
    33361 tgccggtatt aagtttgctg gagggcgatt atgacctttt tgtgctggag ctatccagct
    33421 tccagcttga gacaacttat tcactgaaag caaaagctgc cactgtttta aatattagcg
    33481 aagatcatat ggatcgctac gattcgattg ccgactatcg ccaagcgaaa ttgcggattt
    33541 acgataatgc ggaaaatatc atcgtaaacg gtgaagatgc ccaaacttac ccgatacaag
    33601 cggtcaaaaa agtgattcgt tttgcagaac aaaatgcgga atatagccta cgcaatcaga
    33661 tgctttattc aggcgatgat gcaattatcc aaactaaaca gatgctgatt agcggccgtc
    33721 ataatgagat gaatgcgtta gcggcgatgg cattagcaga agcggcgaat gttcctcgtg
    33781 aaggcattgt gaaagctttg caaatttatg gtggtttgcc acaccgtttc caagcggtgg
    33841 caaccaatga tggcgtgcgt tgggtcaatg attccaaagc gaccaatgtg ggtagcacgg
    33901 tagcggcatt aaacggcttg aatattgccg gtacacttta tttactttta ggtggtgacg
    33961 gcaaggctgc ggatttttcc gaactcaaac cattaattaa taagccaaat attgtctgct
    34021 attgtttcgg gcaagatgga gcggcattgg cagaattaag cgaacaaagt gtgttggttg
    34081 aaacgatgca gcaagcaatt gaagcaatcc gcccacaact gaaaaaaggc gatatggtac
    34141 tgctttctcc ggcttgtgcc agtttagatc agtttaaaaa ctttgagcaa cgtggcgagg
    34201 tatttatggc attagcacaa ggacagcacg cataatgtta gaaagaatta agtcggaatg
    34261 ggataaatgg gtcagattaa ccccttctaa ttcactttat gacagaactc taatttggct
    34321 atttttaggc ctgctaatta tcggttttgt gatggtaaca tctgcttcac tcccagttag
    34381 cacaaggttg aataacgatc cattttattt tgcgattcga gacgggctgt atattattgc
    34441 ttcaattatc ttttgttatg tttttgtaca aatcccaatt gaaaaatggg aaaagcataa
    34501 cttggcactg ttttttattt caatcggttt tttaatcgcc gtactcattt tcggccgctc
    34561 aattaatggt gcagtgcgtt ggattccgtt gggtattctc aactttcagc cggcagaatt
    34621 agccaaatta gcggtcattt gctattttgc cagtttctat gtacgtaaat atgatgaaat
    34681 tcgtaaagaa aaagccagtt tctggcgacc ggcagtgatt ttattcttat tcggcttctt
    34741 gctgattttg cagccggatt taggtagtac ctttgtatta tttgttctca ctttctcaat
    34801 gcttttcatt gtgggggcaa aaattatgca atttatgttt ttaggcgtgg tgggaacggt
    34861 gttatttgcg gtgttgattt taacctcgga ataccgttta aaacgtgtta cctcctttat
    34921 ggatccgttt gctgatgctt atggcgacgg tttccagctc tcgaactcac aaatggcatt
    34981 tggacaaggt gaattttggg gacaaggttt aggtaattcg gtacaaaagc tagaatattt
    35041 gccagaggca catactgact ttgtaatggc ggttattggt gaagaatttg gtttctttgg
    35101 tattgcttgc atcgtacttt tacttattct attaacggtg agagccttaa aaatcagtaa
    35161 agaatctttg gtgctggaag agcgttttaa aggctatatg gcatttggga ttgcgatttg
    35221 ggttttccta caaggcttcg taaacttagg ggtggcatca ggtttgctgc caacgaaggg
    35281 cttaaccttc ccattagtga gttatggtgg ttcgagtttg gtaattatgt cgattgcgat
    35341 tgcagttttg ctacgaattg accacgaaaa ccgtgccgag cgaatcgggc acgcaaaaat
    35401 taaaaccatc tagccgagcg gtctgcaacg ttacaatttg caaaatttta caaaaatgag
    35461 accgcttgta ttagctggaa agcataaaaa ttcagtaaaa tagcacgttt tagaataaaa
    35521 aagagttgaa aatggcgaag aaattattag taatggcagg tggaacaggg gggcacgttt
    35581 tccctgcaat tgccgtagct cgagagctac aaaaacaagg ctggcaaatc cgctggctgg
    35641 ggacgaaaga ccgaatggaa gccactcttg tgccgaaaca cggtattgag attgatttta
    35701 ttgaaatctc aggcttgcgg ggtaaaggcg ttgcagcatt gttgaaagca ccgtttgcta
    35761 tttttaaagc ggtgatgcaa gcccgcaaaa tcattaaaaa ttatcagcct gatgcggtgt
    35821 tgggaatggg cggttatgtg tccggtccgg gcggtattgc ggctaaatta tgtggcgtgc
    35881 cggtgatttt acacgagcaa aacgcagttg ccggcctaac caatgtgtgg ctatccaaaa
    35941 ttgcccgcaa ggtgttacag gctttcccta ctgcttttaa agaggccgaa gtagtaggta
    36001 atcctgtacg tgaagattta tctgctcttc cgctgccgaa tgaacgtttc gccaaacgag
    36061 gctacccgat taacatttta gtaatgggtg gcagccaagg ggcaagagtg atcaaccaaa
    36121 ccgtgccgga agtggcaaaa gtgttgggta ataatgtgtt tatcagccac caagtcggta
    36181 aaggtaattt agccggtatc gaagaagtct atcaacaaac cggcaatggg attgcttccg
    36241 agtttattga tgatatgaaa gccgcgtatg agtgggcaga tttggtgatt tgtcgttccg
    36301 gtgcgttaac ggtgtgtgaa attgccgccg taggtctgcc cgctgtgttt gtgccgttcc
    36361 aacataaaga caggcaacaa tttttaaacg ctaactattt ggcggcagat ggagcagcag
    36421 tgattatcga acagagcgat ttcactccaa aggcattaca gcaagcctta gaacccttaa
    36481 ttgccaatcg ccaactctta ttggatatgt caatgcgagc cagagcaaaa tccaccccaa
    36541 tggcagcaaa acgtgtagcg gaagtgatta ttcaagaagc gaattaactt actacaagcg
    36601 gtcgtttttg gtaaaaattt tgcaaaatgt aaccgcttgc gaatagatta aaaggacaat
    36661 aaatgaaaaa tttccaagat aaagtcaaaa aattagtgcc tgaaatgcgt cgtgtcagcc
    36721 aaattcattt tatcgggatt ggcggagccg gtatgagcgg cattgcggaa gtgttattaa
    36781 acgagggata ccaaatttca ggttcggata ttgcagacag agcggttaca caacgtctat
    36841 ccgcagccgg agcgaaagtc tttatcggac accaagcaga aaatattgct ggggcgagtg
    36901 tggtggtggt ttctactgcg attgatgaaa ccaaccctga aattattgcc gctcgagaag
    36961 ctcgaattcc ggtgattcgc cgagcagaaa tgcttgccga aattatgcgt tttcgccacg
    37021 gcattgcgat tgcaggcaca cacggtaaaa ccactacaac cgcaatgatt tcaatgatct
    37081 acaccgaagc aaaattagat ccgactttcg tgaatggcgg tttggtaaaa tctaccggca
    37141 aaaatgctca tttgggggcg agccgttatt taattgccga agccgatgag agtgatgctt
    37201 ctttcttgca cttgcagcca atggtgtcgg tagtaaccaa cattgagcct gaccatatgg
    37261 acacctatgg tggcgatttt gagaaaatga aagagactta cgtacgattt ttgcgtaact
    37321 tacctttcta cggcttagcg gtaatgtgtg ccgatgatga aacggtaatg gaaattgctc
    37381 ctcgtgtcgg cagacaagtg attacctatg gctttagcga aaatgcggat taccgaattg
    37441 aagattacca gcaaaccggc ttccaagagc attacaccgt agtttgccca agcggtgaac
    37501 gaattgatgt gctgttgaat gtgccgggta tgcacaatgc cttaaatgca accgctgcgt
    37561 tagcggtggc aaaagaagaa gggattggca atgaggcgat tttagctgct ttagctgatt
    37621 tccaaggggc aggacgccgc tttgaccaat taggttcatt tattcgcccg aacggcaaaa
    37681 ttatgttagt tgatgactac ggccaccacc caactgaagt tgatgtaacc attcaagccg
    37741 ctcgttcagg ctgggaaggt aaacgtgttg tgatgatttt ccaaccgcac cgctattctc
    37801 gtacccgtga tttattcgat gaatttgtac aagtactgtc taaagtcgat gccttgattt
    37861 tattggatgt gtatgctgcc ggcgaagctc cgattgtggg ggcagacagt aaagccttag
    37921 cccgttcaat ccgtaattta ggtaaggtcg atccgatttt ggtttcagat accgatcaac
    37981 ttggcgaggt attagatcaa attattcaag acggtgattt aattttggca caaggtgcgg
    38041 gcagtgtaag ccgcatttct cgtggcttgg ctgagagctg gaaaaattaa atactctctc
    38101 cccttgcggg agagagaccg aaaaatactt cgttcagaag tatttttcag agagagggta
    38161 tatatttaaa ggatttcaaa tgagtattaa aaacgaaaaa attgccgtct tattcggtgg
    38221 cgtttcgcaa gagcgtgaag tttcactgaa ttctggtgca gcggtaacag ctgcattaaa
    38281 aagtttaggc tataacgtag aaggcattga tactaaagaa ttcccgattg aaaaattaaa
    38341 agaaaaaggg attcagcgtg tatttaatat tttacacggc ggtattggcg aaaatggcgt
    38401 actgcaaggt gcgttagagc aaatgggtat tccttacacc ggttgtggcg taatggcatc
    38461 agcagtgacg ctggataaat tccggactaa attattatgg aatgcagtag gtttaccaac
    38521 agcggatatg gtggtggttc agcgtggaca agcggtcgat tttgaccaaa ttattgcaaa
    38581 attaggctta ccggtatttg tgaagtcttc gtgtgaaggc tcaagtgtgg gtgtattcaa
    38641 agtaaaaacc aaagaagaat tggcaccggc aattcacgag gcattaaagt ttgattccat
    38701 tgtgttggtg gaagaatttt tagccggtaa tgaatattct gtaccggtat tagacggaca
    38761 agttctaccg gcggtacaag taattccgga cggcgaattt tatgactacc acgcaaaata
    38821 tgtgtcggat aacacccaat atgtagtacc ggcattaaat gacgaacgcc aagcagaggt
    38881 cgccaaatta gtgaaagcgg cttacgatgt ggttggctgt cgaggttgga gccgtattga
    38941 tgtgatggaa gatggcgaag gaaaattccg cttggttgaa gtaaatacca acccgggaat
    39001 gaccagtcac agtattttcc caaaatcagc agcaacagtt ggtatttcct ttgaaaaatt
    39061 agttgaacgc gtattggagt taagtgctta atgggcatct tccgcaagaa gaacagcaca
    39121 aatgtgcgtt tgaatgttct taatccaaaa acacctgttt ctccacgtaa atggctagta
    39181 ttcattaagc cattaattgt gttattatgt acactatttg tattcggtgt gtatagtaat
    39241 tggcaaaata tactcgaaag ttgggataaa acaccgattc gcgcttatgc attaacacat
    39301 aaaacacaat ttacaacaaa tgcagatatt cgtgaaactt tgtctaaaga acctgctcta
    39361 aaaggatatt tcggacaaaa tattcaagag gtcaaagata agcttttatc tataccttgg
    39421 gttaaagatg tggctgttcg taaactttat cccgaccgat taagtattac tttgctagaa
    39481 cataaacccg ttgcaatttg gaatgatact aactttgtct cagaacaagg aaccgtcttt
    39541 agtttaccga aagacagaat cgataaaaat ggacttccat tactctatgg tccagatacg
    39601 gaaggaaaaa gtgtattaga ggcatggaat aaaatccgag ctgatctaaa tgcccgcaac
    39661 ttggatttaa cctccgtttc tgtagataat cgaggttctt ggacaattag gctttctaat
    39721 caagtagaat taagattagg aagaggggaa tggacttcta aaattgatcg ttttgtaaca
    39781 atctttcctg aaattcatat acctgaaggt aatagattag cttatgttga tttacgttat
    39841 ccacatggcg cggcggttgg atttagtcct ataaaaaatt aaactcagtg aacacaaaaa
    39901 cattatgaca aaagcagtag aatcaaaaat tatcgttggt cttgacattg ggacatcaaa
    39961 ggttgttgcc gttgttggtg aagtattacc tgatggtgta attaatgtta tgggagctgg
    40021 cgtttgccct tctaaaggag tcgatcgagg tggcgttatt gatcttgacg ctgccgttaa
    40081 ttctattcaa agagccattg agcaggcgga atctattgcc gattgtcaaa ttatgggtgt
    40141 aacactggca atctcaggtc agcatattgt tggtttaaat gaagcaggat catcaacatt
    40201 atctggaggt attgttaccc aagataatat tgaaagtgct gtggatatgg caaaggcaat
    40261 taaattacaa gatggcttag aaacattaca catgattcca caagaatatc acgtggatag
    40321 attgcctgcg actaaaaatc caattggctt atcgggtatg cgtttacaag ttcaagcaca
    40381 cttaattgct tgccatcaag cttggttaaa aaacttaaaa aatgcggttg aacatgctaa
    40441 attaaaagtc gatcaagttg tattttcagg tcttgcctct agctattctg ttttaactga
    40501 agatgaaaaa gagctgggtg tttgcttaat tgatattggt ggcggtacga tggatgtact
    40561 agtttatacc gatggagcat taagatacag taaagtcatt ccatttgcag gtaataacat
    40621 tactgattat ttggctcgtg tatttacgac atctcgccct gaagctgaaa gccttaaagt
    40681 aggttatggt agcgcaattt cgcctccaac ccataattca gataaaaaaa ttgaggtggc
    40741 aggactaggt ggacggatgg caagaacctt tacccgagct caggtggcaa cggttacctc
    40801 acaatgttat aacgatttgc taaaagttgt tgaagaggaa ttaacccaat tacgccatga
    40861 acttttccaa aaaggaataa aacaagagtt aattgccggt gtagtcatta ctggtggtgg
    40921 ctcacaaatt gaagatattg tagaatgtgc taaatcaatt tttggttcac aagttcgtgt
    40981 tggatatcca ctcaatatta ctggcttaac cgattatgtg aataaaccac aatatgcaac
    41041 agcattaggg cttttacaat atagccacta taattatact gaacaaggta caagtacaga
    41101 ttctaatgaa gatatctttg ctcctattgg tcgtggaacc aagaaaatca ttgacttctt
    41161 caaatcaaaa ttttaatagt gttaaaataa acacgcaggg agataaaaat gtttgaacct
    41221 attattgaac aaacgcaaga tgctatcatt aaggtagtgg gtgttggtgg cggtggcggt
    41281 aatgccgtag acagaatgtc tcgctctgcc gatgatatta aaggtgttga attctttgat
    41341 gtaaatactg atgctcaagt tttaagaaaa agaaccacac gccaaactat ccaaattgga
    41401 gcaagtacaa ctaaaggttt aggtgcaggt gctgatccaa tggtaggaaa acaagctgcc
    41461 gaagaagatc gtgaggctat cgctaatgca ttaaaaggtg caaatatgac ctttattgct
    41521 gttggtatgg gtggaggtac cggtaccgga gcagcccctg ttgttgctca aattgccaaa
    41581 gagcaaggct cactaactgt aggcgttgta acaaaacctt tccgcttcga agggcctcgc
    41641 cgtatgcgct ttgcagacca aggcattaaa gagctttctc aatatgttga ttctcttatt
    41701 ataattccta atgataaatt acgtggttta ggtaagcaaa caacagcagt agatgccttt
    41761 gccgcagcaa atgatgtttt aagtaactgt gtgcttggta ttaccaatat gattaccagt
    41821 tcgggcggtt ctaccggtgc agatattaac gtggacttcg cggatgttcg tacagtaatg
    41881 tctggtaagg gacacgctat gattggtaca ggttttgctg aaggtgaagt tggtgaagga
    41941 cgagcggaaa aagcaatgaa tgatgccatt tctagtccat tacttgaaaa tgtggatatt
    42001 tcaggtgcaa gtggtatgct catcaacatt agtgctggga cagatttctt attagaagaa
    42061 gtttatgcta tgatggactt aatttatggc tttgcaacag aagatgccgc aattgtattt
    42121 ggttgtaact attaccctga aatggatgga aaagtgagtg tcactcttgt cgcaacagga
    42181 ataggccaac ctgaggaagc tctacatatg ccacataagg ctcagccagt atatgcacag
    42241 caaggaaatc atgttcaagc tacacagcct actcagccaa accatgcggt acaacaacca
    42301 aactttactc aaccgtcaag ttatggtcag ccaaatcaat ttagtggtca accacaacca
    42361 ctacaacaac ctgtggctcc aaaaccacaa tcggttgata cagcacaaat ttttaaccca
    42421 aattcaattc cgggttttat gagaaatagt caataacttc cactcatttc ggaagtaagg
    42481 acaaaatatg actaaacaaa gaacattaaa acaagcagca aaagtaacag gtattggctt
    42541 acatagcggc aaaaaagtaa ccttaacgtt acgccctgcc ccagctaata caggagttat
    42601 ttatgcccgt accgatttag agcctgtcgt ttatttccct gcaagcgcag actctattcg
    42661 tgatacacaa ttatgcacat gtatggtaaa tgatgatggt gttcgtattt caacagtaga
    42721 acatttaaac gcagcaatgt cagcattagg cttagataat gttattgttg aagtagatgc
    42781 acctgaaatt ccgattatgg acggtagttc aagcccattt atctatcttt tattagatgc
    42841 cggcatcgaa gaacaagatg caccgaagaa atttattcgc attaaagaaa cagtacgcgt
    42901 tgaagaaggc gataaatggg ccgaattaaa accatacaat aaaggtttta aattgaactt
    42961 cactattgac tttgagcacc cattaatccc caaaaatgta gggagctatt ccattgattt
    43021 ttctgctgag aactttattc agcaactaag tcgagcgaga acctttacat ttatgaagga
    43081 tgtagaatac ttgcaatcta ttggtcttgc attaggtgga agccttgata atgctattgt
    43141 attagatgat taccgaattc taaatgaaga cggtttacga tttaaagatg aactggttcg
    43201 ccataaaatg ctggattcga taggtgactt atatatgtgt ggttataata tcctaggtga
    43261 atttagtgca tataaatcag gtcatggcgt taataataaa ttattaagag ctgtattgga
    43321 aaataaaaac gcttgggagt ttgttacctt tgaagataaa gcagaagtcc cacaaggtta
    43381 ccaagtacaa caagaaattt ttatttagtc taaatggctt aaaagacgaa cagaaatgtt
    43441 cgtcttttat tttgcaaatt ttttctaata tttaaccgct tgcaacccgc ctcattagcg
    43501 attttttcgc ccttatctat ttactgttta aaaaaacagt tgtactatat acccatacag
    43561 ttaatcttgt gtggtgtatt atgcttactc aacttactat taataatttt gcgattgttc
    43621 gccatcttat tcttgaatta aatgaaggta tgtctgttat taccggtgaa accggtgccg
    43681 ggaaatcaat cgctattgat gccttagggc tatgcttggg ttatcgttct gaaagtggga
    43741 tgattagaaa cggttcggac aaagcagata taagtgcaac ctttactatg cagccacata
    43801 gccctgctta tttatggcta caagagcatg aactgcttga tgaagataat cctcaagagt
    43861 gtatcttacg ccgtatgatt aatattgatg gacgctcaaa agcctttgta aataaccgat
    43921 cattacctgt ttcacaactt cgagagttgg ggcaatatct tattcattta aatgggcaac
    43981 acgcaccaca attactccta aaaaacgaat atcaacttga aatagtcgat cattatgcca
    44041 cgttacagcc tctattgatg aagatggcaa agcaatatgg gaattggaaa gaactgaata
    44101 agcaggtaaa aaatttccaa aaacagtgcc aagaaaatga agctcgcaaa cagctattag
    44161 aatatcaagt tgaggaatta gatgaatttg caattaaagc aaacgaattt gaagaaatgg
    44221 aagagaaata caatatcttg tcttcttccg aggagcttac tgagctttca gatgaaatca
    44281 cacgcttatt aagtgatgat gaaatcaatg caaacagtct gatttataaa gcgttacgca
    44341 atgcagaaaa cttggctgag gttgatagcc aatatcaggg cattttagat atgcttaatg
    44401 aggccctgat tcagattcaa gaagcaagct ccgaagtggc tcaattagcc agtaaaattg
    44461 aacaagatcc ggaattacta caggagctgg ataatagaat tgcacaaact atatcgttag
    44521 cacgcaaaca ccatattcaa ccgaatgaat tatggcaata tcacgcgaca ttaaaagcag
    44581 aactacagga attactggat tttgaagata atgaagaaca actcattcaa aatgaaaaac
    44641 aagcttacca agagtgtata acattagcag aagaaattta ccaaaaacgt ctagatgcca
    44701 cacataagct ctctgaacaa gtaacaaaac aaattaaaca cttagcaatg gaaaacggtg
    44761 agttctttat tcatatagaa cataatcttg aaaaaatgtc tgcaaatggt gctgactatg
    44821 tcgagtttaa tttacgcagc aatttaggac aacaacccca accactggca aaaattgctt
    44881 ctggtggtga gctatctcgc atttctctgg cagtacaagt gcttaccgca aataaacttt
    44941 ctacgccaac tattattttt gatgaagttg atgttgggat tagtggctct gctgccacta
    45001 cagtcggaaa gctactgcgt aagctaggcg aaaaatgcca agtattatgt gtaacccatt
    45061 tacctcaagt ggcaagttac ggcaaccagc agtttaatgt acaaaaatat gtagaaaata
    45121 atcaaactga aacgcaaatg acattactca cagaagcaga gcgtattcac gccttggctc
    45181 gactattagg cggaagcgaa atcaccgaaa cggttttagc taatgcaaaa gaaatgctaa
    45241 atttagcgaa aaataacgaa tagccaaaaa gaaaaatcgc atcaatgaat attgatgcga
    45301 ttatgaattt ggtggaggta aacgggatcg aaccgatgac ctcttgcatg ccatgcaagc
    45361 gctctcccaa ctgagctata cccccttaat acaagcggaa taatatgctt tccgcttgaa
    45421 gatgtcaatg gctttaatca agcaaggtgt tgattgatta aaaactagct attcgcctga
    45481 ataaactcaa tcgctttttg taagcgagct aaggttcttt ctttaccgac taatttggcg
    45541 gtgatatcca ttgacggcga ttgcccactt cctgttaccg ctaaacggaa cggcataccg
    45601 actttgccca taccaatttc cagctcggca gcggtttggt tcattgcctc gtggatattt
    45661 tccaccgtcc agtcattgca agcggtcaat ttttccaata attttgcaag tggagcaacg
    45721 gcttcggctt tgaagttttt agcagcagct ttttcgtcgt aagccttaaa ttcttgatag
    45781 aaatagcggc tgctttcggc aagctctttt aaggttttgc aacgttcgcc cagtacaggg
    45841 ataatttctt ccaattttgg accgcttgtg aaatcaatgc cttgagtttg catatgccat
    45901 tcaagatgtt ttgccacata acttggggca agactgcgga tatagtgttg gttcaaccat
    45961 tgtaattttt cagtgttaaa tgcacttgcc gatttactga ctgaatgaat gtcgaacagc
    46021 tcgatcattt cgtcacgaga gaaaatctct tggtcgccgt gtccccagcc taaacgcaca
    46081 aggtagttaa ttaaggcttc cggtagatag ccgtcatcac gatattgcat tacgctcacc
    46141 gcaccgtggc gtttggagag tttttgcccg tcatcgccgt tgatcattga aacgtgggcg
    46201 taggttggga ttggtgctcc taatgctttc aaaatgttga tttggcgtgg tgtattgttg
    46261 atatggtctt caccccgcac aacgtgagta atgcccatat cccagtcgtc caccaccaca
    46321 cagaaattgt aggtcggcga gccgtcagta cggcgaataa tcaagtcatc taattcgcta
    46381 ttggcgattt caattcgccc acgcaccgca tcgtcaaata ctaccgagcc ttcggtcggg
    46441 tttttgaaac gcacaacatg tggttggctc gggtcgtttt gcggattgtg taagcagtgc
    46501 tgatcgtatt tcgctttctc tttatttgct tcttgctctg cacgtacttc gtctaaacgt
    46561 tctttcgagc agtagcaacg gtatgccgtg ccggcttcta acatttggtc gatcacttgg
    46621 ttatagcgat cgaaacgctt ggtttgaaag tatgggccgt gttcccacgc caaatccagc
    46681 cattccatac cctctaaaat agcggcggtt gcctcagggg ttgagcgttc aagatcggta
    46741 tcttcaatac gtaacacaaa ttcaccatca ttatgcttag catataacca agagtagagc
    46801 gcggtcctcg ccccacctac gtgcaagtag ccagttgggc taggtgcaaa acgggtacgg
    46861 acttttacat tcggatctaa ggggaaaagg gtttcaattt tcattttata ttgcctaatt
    46921 gatgattctt aagaaaaact tgtctatttt actacgcttg agggtgaatc atagtaaaaa
    46981 attaaatttg cggggtaaaa aatcagcaaa cggggtaaaa agaacaaaaa gggtttgacg
    47041 aaacagcaaa aatctctata attcacttcc acaacagcgg gcgattagct cagttgggag
    47101 agcacctccc ttacaaggag ggggtcactg gttcgagacc ggtatcgccc accacttcaa
    47161 cttaagtgtt tcgttgttgt atcacaatct aattttgaaa acgggcgatt agctcagttg
    47221 ggagagcacc tcccttacaa ggagggggtc actggttcga gaccggtatc gcccaccact
    47281 tcaacttaag tgtttcgttt tcaaattccc tttgatttta agtctcaagt gggcgattag
    47341 ctcagttggg agagcacctc ccttacaagg agggggtcac tggttcgaga ccggtatcgc
    47401 ccaccactta agactaaaat cataaacttc aaatggcgat ttttcgtatc tgaagggcga
    47461 ttagctcagt tgggagagca cctcccttac aaggaggggg tcactggttc gagaccggta
    47521 tcgcccacca tctatttagt atcaaagcct aaaacttata tcataatcta ataacccctt
    47581 taaagtcata actaaaaagg gaaaataaat tcagatggcc tgttgcatct gcataaatgc
    47641 ctttctacgt gcagaaatta atctttttct atctttaatt tgcttaattt gacgcaggcg
    47701 taatttcctt gtcagtaggg atttcttttt cttcaatttt ctcataagaa gaattctcct
    47761 gttttatttg accatctggt aaattttctt gttcttgcat cttttgctct tcaactcgtt
    47821 gataattaat aatactacca taataacgcc gtatattttc aacatactgt aatgcttcat
    47881 atcctcgagc atagccgtat tttagattag cataataacg tttttccgca agcaatggca
    47941 gattcttttt aacatcaagc caattatccg gatcaccacc aagttgtttt gttaatcgac
    48001 gaagatccaa caagtgcccc aatcccatat tatacgctgc taggctatac caaattctat
    48061 ctgcttgagg aatagtttcc ggcatttgtc tcattagcat atgtaaatat tcagatcccg
    48121 ctttgatact ttgtgcaggg ttagtcctat cagaaatttt cattctatct gccgtatctt
    48181 ttgtcagcat cataattccc ctcactccgg ttggagaagt tgcattagga tcccaatgag
    48241 actcctgata ggcgatagcc gccagcattt gccattctaa ttcaccttta tgtgcccgaa
    48301 aaaggggttc atatttaggt aaaatagttt taattgcatt caaatagctc tgaatatcaa
    48361 cataatcaaa cttagctaaa tgattaaaat atttctcttc tattcttgat atcagcccag
    48421 tttccataga ataagacata aaatccagca gcacagattg cagttcacta taagaactat
    48481 ttgctaaata ccataacact gggttttcat cagttaaatc aaaccctacg gctaaattag
    48541 gattaatatg ctgtgcagca gaaatatcaa ccgacattgc aattgtatgt gaaatttcac
    48601 cttgtgcgac ttttaacaat agctcttctt gtgtaaattt atccgagatc tgccatttta
    48661 gttccggatt atccacctta agctgattta atattggaat taccgcagaa ccggctggaa
    48721 taatcacatt ttcccgcaaa tcggaaagtc gatatggtct agcacttccc tttttataaa
    48781 caagctgcca agaagccgaa taataggaag gacctatctg aaattcatcg tttaattcag
    48841 aatggtaaag cagcccagcg gcagcaatat caacatcatt atttcttaag gcctcaaaca
    48901 atttctcgct actatcataa gttttaatat caagattgac ctttaaatat tgagcaaagg
    48961 ctgttgctaa atcatattca atgccacttt ttccttcagg accaacaaag taagaaagtg
    49021 gatggttgat cattccaacc tttaacgttt tgctttgctg aatttgtgta taatgatttg
    49081 cttctgaatg aatgattttt tgccaaggaa aaatcatatc ccaagcccac agtaacaatg
    49141 ctacggcaat aaaaaggcga gcaattaatc ctttcaattt aaaatcctca ttatttcaag
    49201 ctaagcatta agcctatctg aaattacaaa ttaacggaat aatactttat agtttaatta
    49261 tttattaaaa aaataaaatg ggaatataat ggatataata ta
//
LOCUS       DANLZC010000004        43142 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.004, whole genome shotgun sequence.
ACCESSION   DANLZC010000004 DANLZC010000000
VERSION     DANLZC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 43142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43142
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.004"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            2..1597
                     /gene="pckA"
                     /locus_tag="PXE44_000179"
     CDS             2..1597
                     /gene="pckA"
                     /locus_tag="PXE44_000179"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL5587728.1"
                     /translation="MEQELADLGITNVKEIVRNPSYEQLFEEEMKSDLKGYEKGVLTN
                     LGAVAVDTGIFTGRSPKDKYIVLDETTKDTVWWTSDAAKNDNKPMTQSTWQSLKTLVT
                     DELSNKRLFIIDAFCGASPEHRINVRIITQVAWQAHFVKNMFIRPTEEELKDFKPDFV
                     VMNGSQVTNPNWKEQGLNSENFVAFNITEGVQIIGGTWYGGEMKKGMFSMMNYFLPLK
                     GVASMHCSANVGEEGDVAVFFGLSGTGKTTLSTDPKRKLIGDDEHGWDENGVFNYEGG
                     CYAKTINLSIENEPDIYRAIRRDALLENVVVREDGTIDFDDKSKTENTRVSYPIYHID
                     NIAVPSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEP
                     TPTFSACFGAAFLSLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDTRGII
                     DAILDGSIEKAEMSTLPIFNLAIPTALPGVETHILDPRNTYEDKAQWQAKAEDLAGRF
                     VKNFEKYATNEEGKALIAAGPKL"
     gene            1690..2691
                     /gene="bioB"
                     /locus_tag="PXE44_000180"
     CDS             1690..2691
                     /gene="bioB"
                     /locus_tag="PXE44_000180"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDL5587729.1"
                     /translation="MTTFQLRLKAKDELTPHPTAQYWRKCQVEGLFDLPFMDLVFRAA
                     QVHRENFDANAIQLSTLLSIKTGGCPEDCEYCPQSARYDTGIEKQTMLDVETIVEKAK
                     IAKERGASRFCMGAAWRGPRPKDIKVVSEIINAVKSLGLETCGTFGMLEEGMAEELKQ
                     AGLDYYNHNLDTDPERYNKIIHTRNHEDRMDTLGKVRNAGLKVCCGGIVGMNETRPER
                     AGLIASLANLDPQPESVPINQLIKVEGTPLTNAEDLDWTEFVRTIAVARITMPQSFVR
                     LSAGRTTMPEAMQALCFMAGANSIFYGDKLLTTENPEEDHDRQLMDKLDLYPLQYIA"
     gene            2851..2948
                     /gene="ffs"
                     /locus_tag="PXE44_000181"
     ncRNA           2851..2948
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PXE44_000181"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2983..3690
                     /gene="gloB"
                     /locus_tag="PXE44_000182"
     CDS             2983..3690
                     /gene="gloB"
                     /locus_tag="PXE44_000182"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDL5587730.1"
                     /translation="MLQITPIPALKDNYIWAIRNGQDVIIIDPSEHQPALNFIAKNAL
                     NLTAILITHNHHDHTDGVSGIVEQYPNIPVYGPQEVAEFANVIVSPEQHISLFGYDVR
                     IIESGGHTAGHISYLFGYDYLFCGDALFSGGCGRVFTGDYQAQFDALQRFKNLPNSVK
                     AYPAHEYTKSNLKFAEAVLPPSCVLSEYQEQVDLLRSQNKPTLPTTIGLEMQVNPFMQ
                     AVDLAEFIALRQQKDNF"
     gene            3849..4562
                     /gene="uppS"
                     /locus_tag="PXE44_000183"
     CDS             3849..4562
                     /gene="uppS"
                     /locus_tag="PXE44_000183"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDL5587731.1"
                     /translation="MNFDPQNMPKHVAIIMDGNGRWAKQKGKLRIFGHQNGVKAVRSA
                     VSFAAKHHIQVLTLYAFSSENWSRPETEVSALMSLFMKALDSEVKQLHKNNIRLKIIG
                     DKSAFSENLQKRIAESEKLTENNTGLILNIAANYGGYWDIVQAAQQLAVHVKSEQLAV
                     EQITPALFQQHLVTENQPQVDLLIRTSGEQRISNFLLWQIAYAELFFSPVLWPDFNDE
                     AFSDAIYAYQQRERRFGGC"
     gene            4586..5455
                     /locus_tag="PXE44_000184"
     CDS             4586..5455
                     /locus_tag="PXE44_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL5587732.1"
                     /translation="MLKERVLSAIIMIIAVLAAIFWLSPLPLTLVLSAIVVAAMWEWA
                     QFAGFKHPVPRAIVAMVTICLLLFAIVANTDYIRATRFITDETTPILFIGCIWWLIAL
                     VLVATYPNSAKLWEKSVAAKFLFGLATLVPFLIGTLALRFHNYAFDPYQGTYLFLYVC
                     LLVWGADSGAYFAGRAFGKRKLAPKVSPGKSWEGVIGGVLTSSLIAFTFLQLTPANVF
                     GKALATTPFILVSVGMVAISVLGDLAESMFKRQAGIKDSSQLIPGHGGILDRIDSLTA
                     AVPFFATFFFFVL"
     gene            5459..6769
                     /gene="rseP"
                     /locus_tag="PXE44_000185"
     CDS             5459..6769
                     /gene="rseP"
                     /locus_tag="PXE44_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL5587733.1"
                     /translation="MTSIIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVL
                     FKKKDKQGTEFVFSLIPLGGYVQMWNDETDINAPAQQALNTKSVLQRAFIIIAGPAAN
                     FIFAILAYWVVFIAGIPTVKPVIGEILPNTIASQARIPTELEITKIGHQSIQDWESAS
                     LALVGFVGNKNVPLEGNINGNSTQKYELDLSNWSIDSAKENPLTALGIRPKSAKVTAE
                     IKQVVENSPASQVGIQAGDRILQINQQPFNWFNLVELVQAGKPIELKIEQRGQIKDLV
                     VQPEKKDERYIIGVIPSYEPLADKYRTELKYDILTAFYKSIEKVWSLTQTILQFIGNL
                     ISGDLSIKNLGGPISMAKGAGATAEIGLVYYLSFMALISVNLGVMNLFPLLPLDGGQL
                     VLLAVEAIRGKALSEKIQLKFQQIGFAFVLSLMLFAFANDIIHF"
     gene            6884..9265
                     /gene="bamA"
                     /locus_tag="PXE44_000186"
     CDS             6884..9265
                     /gene="bamA"
                     /locus_tag="PXE44_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDL5587734.1"
                     /translation="MKKLLLSSLLIANGVVAAPFVVKDIRVEGVQPTTGESIISSIPV
                     RVGQTATDTDVSNVVRHLFSQQRFADVRATREGNTLVIKVAEKPIIGKVEIEGNQAIP
                     KDALEQNLKANLINQGEIFDAAKLEAFKEGLIEHYHSTGRYEAKIDTSVSNNTEGGVN
                     IKLAINEGEVAKAKTIKFEGNNAFSDKKLLENLDIQPDVSWWNIFESSKFEQTAYQKD
                     LETLRDFYLNRGYAKLAIQDTDVQFNDNKTEVALTYKLDEGSQYHVSEMRIIGNTANM
                     DSQLNALLKEFKPGQLFRKSDLTQIEEEIKSILGDAGYGSAKVDIYPQFNEENKTVKI
                     SFVVDAGRRIYVRKIRFEGNGVTADSTLRREMRQQEGAWLSTNKASLGKARLERTGFY
                     ESVDMSMPNVEGTADQVDVVYKIKERNTGSINFGIGYGTKNGISYQAGIKQDNFLGMG
                     STISLNGTRNNYGTSVNLGYTEPYFTKDGVSLGGNIFYEDYDNSDNDSMASYKRQTYG
                     INGTLGFPVNENNSYYLGLGYTHDKIKNAAREYTREKYVKSMNFEFDPAADYYKKIKA
                     DDFDFSLGWNYNSLNRGYFPTEGTTANIGGKITIPGSDNKYYRVSADFRNYYPLNREH
                     KWVISTKASIAYTNGFGGKEVPFYQLYTAGGIGTLRGFSYGAVGPQAIYYNTKSGKFD
                     SMNNDIIGGNALAAASLELITPTPFVSEKYQHNVRTSLFVDAASAWNTKWKKDEYTNL
                     PDYGDFKRFRASAGIAFQWMSPIGPLSFSYAKPIRKYDNDDIEQFQFNIGSSF"
     gene            9345..10136
                     /locus_tag="PXE44_000187"
     CDS             9345..10136
                     /locus_tag="PXE44_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HDL5587735.1"
                     /translation="MKKLFKMTGLAVALASTTGIANAADTIGFVDPSYVLQNHPVLLD
                     ASAKFDKFMKESQEKFAADEKKLADENKTLTAERNKINEDAQKLQNEQKNVEASIKKK
                     VAALEKDAPRLRSKEIQARQNAIQKEANAFQAKVTAIQKREADFAKKAEAFQKRADEF
                     QKRIEQANKENGGINPQEAQKQAVDEVNATIKEIAKSKGYTLVLQPQVALYAEDEGKD
                     ITEAVLDAIVKKHPEIKIEKPATEAQPATEKETKAEETKPEEAKK"
     gene            10136..11161
                     /gene="lpxD"
                     /locus_tag="PXE44_000188"
     CDS             10136..11161
                     /gene="lpxD"
                     /locus_tag="PXE44_000188"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDL5587736.1"
                     /translation="MANFRLIDLAEHIGGTLRGNADLAIQSIAALDKADSSQITFISN
                     AKYRENLTACNAGAIIVSPADVEFCRADQNLIIVANPYVAYAQLAQYMDSTPKAASEI
                     HPSAVISPEAKLGNNVSVGANAVIESGVELGDDAVIGAGCFVGKNSKIGARTQLWANV
                     SVYHNVQIGADCLIQSSTVIGSDGFGYANDKGQWIKIPQTGGVIIGNRVEIGACTCID
                     RGALDPTVIEDNVIIDNLCQIAHNVHIGFGTAVAGGVIMAGSLKVGRFCQIGGASVIN
                     GHMEICDGAIITGMGMVMRPITEKGIYSSGIPLQTNKEWRKTAALVMNIDEMNKRLKS
                     LEKRFND"
     gene            11190..11648
                     /gene="fabZ"
                     /locus_tag="PXE44_000189"
     CDS             11190..11648
                     /gene="fabZ"
                     /locus_tag="PXE44_000189"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL5587737.1"
                     /translation="MTTENREPNIIEVTEIMDMLPHRYPFLLVDRVTDYEEGKWLKAI
                     KNVTFNEPCFTGHFPGAPIFPGVLILEAMAQATGVLAVATYGKLSEDELYYFAAIDNA
                     RFKRPVVPGDQLQLEVEFLKEVRGITKFTGKAYVDGKLVCEADLMCARRK"
     gene            11682..12476
                     /gene="lpxA"
                     /locus_tag="PXE44_000190"
     CDS             11682..12476
                     /gene="lpxA"
                     /locus_tag="PXE44_000190"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDL5587738.1"
                     /translation="MRLIDSTAKISPLAIIEEGAEIGAHVEIGPFSVIGKDVKIGART
                     KIHSNVVINGMTEIGEDNHIFQFASIGEINQDLKYQGEPTKVVIGNRNRIRESVTIHR
                     GTVQGGGVTKIGDDNLFMINTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSA
                     IHQFVVIGSHVMLGGGSMVSQDVPPYVMAQGNHARPFGINFEGLKRRGFDKPTMHAIR
                     KVYKLIYSSGKTLEECLVEIEQIAATEPAIAIFKQFFKRSTRGIIR"
     gene            complement(12514..14514)
                     /gene="mnmC"
                     /locus_tag="PXE44_000191"
     CDS             complement(12514..14514)
                     /gene="mnmC"
                     /locus_tag="PXE44_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDL5587739.1"
                     /translation="MGKLTFATLTFNDADTPVSEQFDDIYFSTQDGLAESYYVFQEGN
                     QLWEKWQSHPKEAFVIAETGFGTGLNFLAVADKFQQFRAAFPQSKLKRLYFISFEKFP
                     LTASQLADIHQRYPQFATLSENLTACWQPRQIGCQRYHFEQVYLDLWFGEIAENLPQL
                     GDLYSGTIDAWFLDGFSPDKNPEMWNEQLYLQMFRLSANGGSFATFTASSMVRRGLEA
                     AGFAVKKRKGFGKKREMLWGEKPLDSQVSEPNYPYFYAKNETADDIAIIGGGVASFFV
                     ALSLLEKGKQVTLYCKDETLAINASGNLQGAIYPQLSDDDERNVQFYIHSFDYALQRL
                     QQLKSNIEFEHNLSGVVLYAYDEKTAKKLEKMVEQTADDTLLKSCSQAELSEKLGLSV
                     PNSGAFIPQAGWLSPVQLVRNGFTWLEQKGLKIVLNHKVEHLTFSNGMWQWQTRGETV
                     RHQAVVLANGHTLNEFSQANGIPLYPVRGQVSQIPTTSELQKLKCVLCYDGYLTPMSA
                     YGTHCIGASHIRDNAETEFSLSEHWENVAKLQQNVTVCEWTQGIDLSENKAKVGIRAA
                     FRDRVPMVGAVHHFEQQKSQYANLYNQLRRKQAVENAEIFTNLYMVNGLSSRGLTTAP
                     LLGELLASLIVNEPVPISEDIWHNLSPNRAWIRKLLKGSTVA"
     gene            complement(14739..16175)
                     /gene="trpCF"
                     /locus_tag="PXE44_000192"
     CDS             complement(14739..16175)
                     /gene="trpCF"
                     /locus_tag="PXE44_000192"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDL5587740.1"
                     /translation="MQNIVQDNAQPNQPTILQKIVQDKAIWVAQKEQAFPLSEFQHKI
                     VKSDHDFYAALAKGTHQEPAYILECKKASPSKGLIRAEFDLDSIAQVYKNYAAAISVL
                     TDEQYFQGDFRYIDQVKRQTTQPILCKDFMISAYQVYLARFYNADAILLMLSVVNDET
                     YRELATLAHELGMGVLTETSTEAEFERALALGAKVIGVNNRDLHTLTIDMNRIVRLVE
                     KYQAQISADVCLISESGINDHQQVKNIKPYVHAFLIGSSLMGSADLNNAVRAVIFGEN
                     KVCGLTRVQDVKAVYEQGALYGGLIFAEGSPRQLSLRQAQELVVNAPLRYVGVFQNQA
                     VEFVEKMAKQLELFAVQLHGSEDEQYIAELAEKLGSKTQIWKALSVDVEVENLEFNDN
                     PQIARYVLDSKVGNQQGGTGKTFNWALIPETLKQKALLAGGIGTENIEQALAQGCVGL
                     DLNSGVESAKGVKDLQKLTACFGKIIQA"
     gene            16263..16574
                     /locus_tag="PXE44_000193"
     CDS             16263..16574
                     /locus_tag="PXE44_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDL5587741.1"
                     /translation="MKSQVIQHLSYLEIALRVNQLWEATPPSPEALANEQPFCVETLT
                     PTQWLQWIFIPRMRAILDANADLPRNFAITPYLEEALKNEGYLQAICEPLLGLEKLLK
                     D"
     gene            16728..17456
                     /gene="truC"
                     /locus_tag="PXE44_000194"
     CDS             16728..17456
                     /gene="truC"
                     /locus_tag="PXE44_000194"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDL5587742.1"
                     /translation="MTLDILYQDNSLIAINKPAGVLVHRSWLDKHETVFAMQTLRDQI
                     GQHVFPIHRLDRPTSGVLLFALNSETARIMSEQFEQHLVQKTYLAVVRGYLQGSAQID
                     YPLKVILDKIADKFSQEKEAQAAITDYKGLATVEMPYPVGKHQTSRYSLVELSPKTGR
                     KHQLRRHLKHLFHPIMGDSKYGDLHQNRALVEKSGVSRLMLHAHKLQFQHPKNLQKIE
                     IIASLDEQWQRLFAFFDWNFTQYY"
     gene            17474..17638
                     /locus_tag="PXE44_000195"
     CDS             17474..17638
                     /locus_tag="PXE44_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDL5587743.1"
                     /translation="MDNSLLSLTHEQQQAAVEEIQKLMAQGISAGEAIQIIANQLRDK
                     YQILEKSNNE"
     gene            17631..18107
                     /locus_tag="PXE44_000196"
     CDS             17631..18107
                     /locus_tag="PXE44_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587744.1"
                     /translation="MNKLWTVLALTITSHYSFAAAEKIVTKDLPAEGKPAVVLQVFDE
                     SEKIAKPIKGNKLFISKKQTRLCWHSINLPVQNKVTIAEAFYAPAAMKLASPGSKINS
                     SADKKNHTIITEINLANNQVLNRCWGFDKTDPVGKYKMEIQINDHIFKGLEFEIVK"
     gene            complement(18144..18995)
                     /locus_tag="PXE44_000197"
     CDS             complement(18144..18995)
                     /locus_tag="PXE44_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL5587745.1"
                     /translation="MENQKQAEIELKIMLEQQNVALVENYLNQTSPFPVLAHQTELLG
                     NTYYDTPEQFFATQKMGLRVRSVNQQYEITLKTQGEIVGGLHIRPEYNLALPNSQPDF
                     QTLVEKYQLPFENSEQIAESLQAIFSTDFTRKTWLVQVGMSDIEVALDQGIIKNHFGE
                     EPICELEFEIKQGNLGDLFTLVEAMPKADGMWLSNLSKAQRGYLLGQAVKWEQKIATA
                     MDGENSEKLTQLLADFICVGNESLEVLVRFNQCTQQQFSNWQQARAFVKSKDYLLHNL
                     TNLKTMI"
     gene            19056..20465
                     /locus_tag="PXE44_000198"
     CDS             19056..20465
                     /locus_tag="PXE44_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDL5587746.1"
                     /translation="MFSKIHHKLNNFVQRGLDNHLRLAVTGLSRSGKTAFITSLVDQL
                     LHINKGGNSHLNLFAPARHGQILSVKRVEQGDLTIPRFEYDKNRQSLENQPPQWPEST
                     TGISEIRLAIRYQRQHSLLRHIKETGTLYLDIFDYPGEWLLDLPLLSLSYKEWSQTQQ
                     TIHKDERAELATPWLQAVKKLDLFAKTDENLLAEFSQQYTDYLLACKAQGMQYIQPGR
                     FVLPTENAKGAPVFQFFPLLDLSEAEWEKLEQSDRNSIFHTLKKRYEHYQKKIVKPFY
                     EDYFSQFDRQVILADCLTPLNYGYNEFMEMKLGLQQLFKHFHYGNRSLFHRLFSSNID
                     KLLFIATKADHITSDQVPNLESLMRQLVQEGGRHAEFDGIETGYLAISSIQATDAVKV
                     TQQDKIIKAIRGIRSSDRKQVTLYPGSVPNRIPDKNYWQFNRFEFDQFEPKQLDYSQA
                     IPHFRMDSVLQFLLADMFE"
     gene            20511..20861
                     /locus_tag="PXE44_000199"
     CDS             20511..20861
                     /locus_tag="PXE44_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5587747.1"
                     /translation="MNTVISQRKPIHWVGSTLDDIREFPDNIKRELGFDLDLVQQGLL
                     PRDFKAMANLGSGVMEIRVKDPSGAFRLVYLAKFKDAIYCLHAFQKKTQKTAPKDIAV
                     IKARYAAVKELENE"
     gene            20854..21177
                     /locus_tag="PXE44_000200"
     CDS             20854..21177
                     /locus_tag="PXE44_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5587748.1"
                     /translation="MNNIDTKIRHTTEAQGNVFEDLGFPIDEAQKLKSASQQLIETKL
                     MLMNEMSNWIDKNNLKQSEAASILGVSRPRISDMVNGKLEKFTLDTLVTFVAKTGKTI
                     QLQLS"
     gene            21285..21764
                     /gene="smpB"
                     /locus_tag="PXE44_000201"
     CDS             21285..21764
                     /gene="smpB"
                     /locus_tag="PXE44_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL5587749.1"
                     /translation="MAKKPKVASNTIALNKRARHEYFIEDEIEAGLELQGWEVKALRA
                     GKGNISDSYVIFRHGEAFLFGAMITPLNMASTHIVSDPTRTRKLLLNKRELDSLFGKV
                     NREGYTIVATSMYWKNAWVKVKIGLAKGKKLHDKREDIKDREWQVAKQRIMKNANRG"
     gene            21967..23274
                     /gene="serS"
                     /locus_tag="PXE44_000202"
     CDS             21967..23274
                     /gene="serS"
                     /locus_tag="PXE44_000202"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL5587750.1"
                     /translation="MIDQNLLRTNLEDVATALKIKRNFILDVERVKTLEEQRKTLQVK
                     TETLQAERNTRSKNIGAAKARGEDISALLAEVDSMGNELDTAKVELDKVQAEIRELLL
                     SVPNLPADEVPLGKDDSENLEVLRWGEPRQFDFEVQDHVTLGENLNGLDFAAGVKLTA
                     SRFVVMKGKLARLHRALSQFMLDLHTEQHGYVETNVPFLVNHDTLYGTGQLPKFGEDL
                     FHTQPLTGQDPNEVQRPFGLIPTAEVPVTNLVRDEIIDEDSLPLRYTAHTPCFRAEAG
                     SYGRDTRGLIRMHQFEKVEMVQIVAPEKSMEALEELTGHAEKVLQLLGLPYRKVLLCT
                     GDMGFGAAKTYDLEVWLPAQNTYREISSCSNMWDFQARRMSARCKAKGDKKTRLVHTL
                     NGSGLAVGRTLVAVLENYQNADGSITVPEVLRPYMGGLEVITA"
     gene            complement(23333..23659)
                     /locus_tag="PXE44_000203"
     CDS             complement(23333..23659)
                     /locus_tag="PXE44_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587751.1"
                     /translation="MVSVDFKNNLLEFIFYGLFSEHTQTNIINGLQFYPKAGVAFRKD
                     LRFRKRAVYQREIMVFYQLDKIDEPKKVTIIAFRLGRMKNPIRLDTVQNRRVVTEIFM
                     NYPEKD"
     gene            complement(23652..23864)
                     /locus_tag="PXE44_000204"
     CDS             complement(23652..23864)
                     /locus_tag="PXE44_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587752.1"
                     /translation="MSIVIVDTDFVVHKEDEEWTTEEIKMIEEHLLEEQSAQYQVKRE
                     VPKQFYADIAERLSRGDLTAWEQLDG"
     gene            24051..26636
                     /gene="leuS"
                     /locus_tag="PXE44_000205"
     CDS             24051..26636
                     /gene="leuS"
                     /locus_tag="PXE44_000205"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL5587753.1"
                     /translation="MQQQYNPSEIEPKVQRYWAENKVFKAVKDVTKEKYYCLSMLPYP
                     SGKLHMGHVRNYTIGDVVSRYQRMIGKNVLQPMGWDAFGLPAEGAAVKNNTAPAKWTY
                     ENIEYMKGQLKMLGFSYDWDREVTTCRPEYYKWEQWFFTELYKKGLVYKKTSTVNWCP
                     NDETVLANEQVHEGCCWRCDTPVEQREIPQWFIKITDYAEELLNQLDNLPQWPDQVKT
                     MQRNWIGRSEGVEITFKIAGSNVELPVYTTRPDTFFGVSYVAIAAAHPLAELAAQNNP
                     ALAEFIREAKNTKVAEAELATMEKKGMATGLFAIHPLTGKEVPVWVANFVLMHYGTGA
                     VMAVPAHDERDFEFAQKYDLQINQVIQPLDGSEWDFSKAAYTEHGKLINSAEFDGLEF
                     DVAFTAIADKLEAMGVGKRQVNFRLRDWGVSRQRYWGAPIPMMTTEEGEVVTVPLQDL
                     PVILPEDVVMNGVQSPIKADPEWAKTTYNGKPALRETDTFDTFMESSWYYARYTCPQY
                     HEGMLDSDEANYWLPVDQYIGGIEHATMHLLYFRFFHKLLRDAGILNSDEPATKLLCQ
                     GMVLADAFYYTSPTNERIWVSPTQVTLERDEKGRIIKATDPQGRELVHSGMTKMSKSK
                     NNGIDPQEMVEKYGADTVRLFMMFASPAEMTLEWQESGVEGAKRFLGRVWNLVYSYAQ
                     NPATTALDVNALDKDQKELRRDVHKTIAKVSDDIGRRQTFNTAIAAVMELMNKLTKAP
                     LETEQDKAVMAEALSAVVRMLYPITPHICFELWQVLGNEQTIDFAPWVQADESAMVED
                     EKLVVVQVNGKVRGKITVSATATEDEVKAIAKADPNVAKFLDGQQIVKEIYVPFKMLS
                     FAVKA"
     gene            26836..27324
                     /locus_tag="PXE44_000206"
     CDS             26836..27324
                     /locus_tag="PXE44_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587754.1"
                     /translation="MIKKLSLIAVFALTACGWHFKNNEVLPESFRTLTFESADQHSEM
                     SRILRNQLQLSDVKLVPSTANVAKLRLVSTSTDSKVVSVFKQAREAEKILTLNVDAIA
                     DIPNKGQYPLEVSVHRTFFDDSRAALAKSAEKEMIMKDMYEQASRQLIIKMAGLKKEL
                     SK"
     gene            27367..28392
                     /gene="holA"
                     /locus_tag="PXE44_000207"
     CDS             27367..28392
                     /gene="holA"
                     /locus_tag="PXE44_000207"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL5587755.1"
                     /translation="MQKIFPETLQFSLNKGLQPFYLLTGNDLLLVNETKDAIIHTARL
                     NGFDEKKEVEIKNDTNWEELFEAAQSMGLFFARQIMVLNFPDSPTATQFKKLAELCAF
                     IHSDLLFILHLPKFSKTMEKQAWFTQIELNLVQINCQTPELSKLPQWVSHRAKAMNLQ
                     IENEANQLLCYSYEGNLLALKQALQLLQLNATDGKITLTKAQEVVEHSAQFTPFQWID
                     ALFEGKIQRAQRILYHLQNEEVQAVVLLRIVQKELMILLEITRNPTAVVSSEQSLFIG
                     NLRTEFDRLKIWQNRRPFYNQITQRLTYQKLYALIQQLAELERKVKQEFSDDIWQELE
                     RFSAQFT"
     gene            complement(28393..28578)
                     /gene="yacG"
                     /locus_tag="PXE44_000208"
     CDS             complement(28393..28578)
                     /gene="yacG"
                     /locus_tag="PXE44_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDL5587756.1"
                     /translation="MPETIVNCPTCEKEVVWSKESKYRPFCSERCQIIDLGDWAAEKH
                     SIASEEAELFSEDLEKY"
     gene            complement(28560..29195)
                     /gene="coaE"
                     /locus_tag="PXE44_000209"
     CDS             complement(28560..29195)
                     /gene="coaE"
                     /locus_tag="PXE44_000209"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL5587757.1"
                     /translation="MTYVVGLTGGIGSGKTTVSDLFSALGVEVIDADVVARQVVEPGT
                     PLLAKIVEHFGEEILTAEKALERTKLRQIVFNNEEEKTWLNNLLHPAIREEMVKKLQE
                     STACYVIWVVPLLIENKLTEYCDRILVVDVSPEIQLERATKRDNSKIETIKNIMAAQV
                     SREERLSYADDVIENNLPFEQGLLLIKEQVQALHYKYLALAKKEKECQKLL"
     gene            complement(29231..29887)
                     /locus_tag="PXE44_000210"
     CDS             complement(29231..29887)
                     /locus_tag="PXE44_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL5587758.1"
                     /translation="MLILFSLFIAVWLKAEIPRFAIRVNRQIYQETTSLVNIGLTEQQ
                     FLAQSVLQPKQPRSANLFFLLFPLIMLCSESPIIGTIFILLCFLSLLDMCYYLTDIRY
                     LVLIFLLVLWESLADFQHQTFTFTLAFCLLIGLVSQLMFKKEAFGSGDMLLLVALSPL
                     FRLEKMLLLILTASLLGIGYYLAYWLMKKQKLAKLAFIPFISTAMLLVLMLESRGINY
                     "
     gene            complement(29890..31086)
                     /locus_tag="PXE44_000211"
     CDS             complement(29890..31086)
                     /locus_tag="PXE44_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDL5587759.1"
                     /translation="MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIR
                     ISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQALSMLLQNCRNIRLYLWLNELIQ
                     HIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
                     KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGT
                     VLLGTLLLAVIFYLFFAKKSRLVAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLN
                     AHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGINPNVFGAEMAQMLEV
                     GEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFD
                     MGALVE"
     gene            complement(31083..31535)
                     /locus_tag="PXE44_000212"
     CDS             complement(31083..31535)
                     /locus_tag="PXE44_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HDL5587760.1"
                     /translation="MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAIPLQETEE
                     QLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSDELKFLLNALSPEQSSRQSEIYEEN
                     TAYSLAHSEQVDRNDPIIQILENLFKFCLQHNASDIHFEPQKEKLLRQ"
     gene            complement(31556..32035)
                     /gene="ppdD"
                     /locus_tag="PXE44_000213"
     CDS             complement(31556..32035)
                     /gene="ppdD"
                     /locus_tag="PXE44_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDL5587761.1"
                     /translation="MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNS
                     YTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIA
                     VTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG"
     gene            32216..33595
                     /gene="radA"
                     /locus_tag="PXE44_000214"
     CDS             32216..33595
                     /gene="radA"
                     /locus_tag="PXE44_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL5587762.1"
                     /translation="MAKAPKTAYVCNDCGAEYSRWMGQCKECKAWNTISEVRLISSKE
                     AAKSDRFSGYAGETSGKVQTLSDISLQEVPRFSSGFNELDRVLGGGVVPGSAILIGGH
                     PGAGKSTLLLQVMCGLAQHLPTLYVTGEESLQQVAMRANHLGLPTANLKMLSETSVEH
                     ICNIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVG
                     HVTKDGTLAGPKVLEHAIDASLLLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGL
                     KEVKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHSMLANPRRVAVGLDH
                     NRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRPLPQD
                     LVIFGEVGLAGEIRPVTSGQERISEAAKHGFKRAIIPHGNVPKKAIKDMQVFTVKKLS
                     DALDILYNL"
     gene            complement(33650..33880)
                     /locus_tag="PXE44_000215"
     CDS             complement(33650..33880)
                     /locus_tag="PXE44_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDL5587763.1"
                     /translation="MDYVEILGYVAMILVACSFLLKDVIKLRVVNSLGCACFVVYGLL
                     IGSIPVTGLNALVVCINLYYISKGNRPTAAHS"
     gene            complement(34000..34494)
                     /gene="folA"
                     /locus_tag="PXE44_000216"
     CDS             complement(34000..34494)
                     /gene="folA"
                     /locus_tag="PXE44_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDL5587764.1"
                     /translation="MKISIIVARTINKVIGKDNQMPWHLPVDLAWFRQNTLGKPVIMG
                     RKTYESIGRLLPKRPNIILSRSGFTVEGAYAVQSLEQAVELAKNFANTAEIMIIGGGE
                     LFKQALPLAERLYLTEIQADIEGDTFFEFDEQNWALVEEKWSEIDENNPFRCRFMCLE
                     RKSV"
     gene            34570..34725
                     /locus_tag="PXE44_000217"
     CDS             34570..34725
                     /locus_tag="PXE44_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl kinase"
                     /protein_id="HDL5587765.1"
                     /translation="MATLFLVTSEQIMSKTIVIKFGTSTLTHGTKSLSRPYMLELVKL
                     NYTNSIV"
     gene            34722..35690
                     /gene="proB"
                     /locus_tag="PXE44_000218"
     CDS             34722..35690
                     /gene="proB"
                     /locus_tag="PXE44_000218"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL5587766.1"
                     /translation="MIVVTSGAAAAGRDYLGHPELPKTLASKQMLAAVGQSQLIRVWE
                     NLFDIYNIHIGQMLLTRADLDDRERFLNARDTLDALLAQKIIPVINENDAVATEEFKV
                     GDNDNLSALVAILAQADQLYLLTDQEGLFSADPRSNPDAKLLSVVEKITPEIRQIAGG
                     SSTGLGTGGMSTKITAADIATRSGVETIIASGSRPNVLVDLANGQNIGSKFLVQSDRL
                     EGRKRWLFAAPQVGEIVVDFGAEQALLQHKSLLPVGVSAIKGEFTRGEVIKIFSQDGK
                     ALALGIARYSSEALLRIKGKKSKEIESLLGYEFGSVAVHSDEMVVY"
     gene            35690..36454
                     /locus_tag="PXE44_000219"
     CDS             35690..36454
                     /locus_tag="PXE44_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDL5587767.1"
                     /translation="MELGIDVLAILFAVATLAGFIDAIAGGGGLLTIPALMAAGLPPA
                     MALGTNKLQACGGSLSSSLYFIRKKAVNLKQIWLLILCTFLGSAAGTIFVQMINVDFL
                     KTLLPFLILIIGIYFLVSPKIGDEDRKQRISFTLFAFTAAVALGFYDGMFGPATGSFM
                     TLAFILLLGFNLSKAVAHAKVINFTSNFASLIFFTLGGAVMWKIGLIMMVGQFIGGTL
                     GAKMVMTKGKKLIRPMLVTMSFMMVGKMLYEQGIFN"
     gene            36457..37089
                     /gene="nth"
                     /locus_tag="PXE44_000220"
     CDS             36457..37089
                     /gene="nth"
                     /locus_tag="PXE44_000220"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL5587768.1"
                     /translation="MNKAKRIEILTRLRNENPKPTTELNYSNPFELLIAVILSAQATD
                     KGVNKATDKLFPVANTPQAILDLGVDGLKEYIKTIGLFNSKAENIIKTCRDLIEKHNG
                     EVPEDRDALEALAGVGRKTANVVMNTAFGHPTIAVDTHIFRVSNRTNFAPGKNVVQVE
                     EKLLKVVPDEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEFKEKTE"
     gene            37286..38536
                     /locus_tag="PXE44_000221"
     CDS             37286..38536
                     /locus_tag="PXE44_000221"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HDL5587769.1"
                     /translation="MDIYLVGGAVRDQLLGLPVKDRDFLVVGATAEQLLAQGFQQVGA
                     DFPVFLHPETKEEYALARQERKNGNGYNGFICDFSPNVTLEQDLIRRDLTINAIAQDE
                     NSTLFDPYGGVQDLENRLLRHVSPAFSEDPLRVLRVARFAARFHSFGFTIAPETLKLM
                     REMVQSGELKHLTAERVWLETQKAFETDNPHVYFDILRLIGALKVLFPELDRLFGVPQ
                     PPQHHPEIDSGKHTLMVLQQAKRLAKKAENPTALLFAALCHDLGKGLTPADILPHHYG
                     HEVKGIQPTRELANRLKIPTEIKDFALLVTEYHTHCHKMAELRPETVIKLFNSLDVWR
                     KPNRFFDFLFACEADARGRLGFEQRNYPQPELAKKYYQAAIEIDVQQVIQDGFEKQAI
                     REELNKRRVYAVRSIKLDSDLINH"
     gene            complement(38637..39572)
                     /locus_tag="PXE44_000222"
     CDS             complement(38637..39572)
                     /locus_tag="PXE44_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL5587770.1"
                     /translation="MSESKPFPITTGFFAMPLGLGALGLAWLNATPITPYATTIGNII
                     AGTSVILWVALLGCYVYRVFFCFEQIKAEFMHPVQCCLFSLVTMTTMISGDVLYVWDF
                     YLGELLIFLGVIVHLIFLVFIIGGLWKGTQFLPETLHPVHYLPPIAGSFTMASSLSLI
                     GYSEIASLFLGIGIISWLVYEPTMLQRIRLSPLFPIARPSLGIVLAPAFVGASAYFSM
                     GGELDLLAKMLWGYGFLQFCFILRNLKWIAENGFSMGFWAFSFGLAAMARGAIEFYQF
                     EPLSFIGLAGFTIANFGIGLLLIFTLKLWLQQSRA"
     gene            complement(39701..39895)
                     /gene="iscX"
                     /locus_tag="PXE44_000223"
     CDS             complement(39701..39895)
                     /gene="iscX"
                     /locus_tag="PXE44_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDL5587771.1"
                     /translation="MKWTDTRMIAENLYDMNPDLDPTTVRFTDMHKWICEMEDFDDDP
                     EASNEHILEAILTIWLEEYE"
     gene            complement(39895..40236)
                     /gene="fdx"
                     /locus_tag="PXE44_000224"
     CDS             complement(39895..40236)
                     /gene="fdx"
                     /locus_tag="PXE44_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDL5587772.1"
                     /translation="MPKIVFLPHEEFCPEGMVVEAKAGDNLLEVAHEAGVEIHHACDC
                     SCACTTCHVVIREGFDSLNDTSDQEEDMLDKAWGLEIDSRLSCQCIVGEEDLVVEIPK
                     YNLNHANEAAH"
     gene            complement(40255..40620)
                     /locus_tag="PXE44_000225"
     CDS             complement(40255..40620)
                     /locus_tag="PXE44_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 51 family protein"
                     /protein_id="HDL5587773.1"
                     /translation="MSNYVKTEISEQFRTVAVEFDLEDNRIFELGERINTEYDVAYMN
                     GYNWAAFLRAYLSIYRPSLLELLEEDPDVGCYYVTYPLSEKSKEKARELKKIIVYLVE
                     NEEEILNFIGEHLDNIEWD"
     gene            complement(40630..42483)
                     /gene="hscA"
                     /locus_tag="PXE44_000226"
     CDS             complement(40630..42483)
                     /gene="hscA"
                     /locus_tag="PXE44_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDL5587774.1"
                     /translation="MALLQISEPGQTAAPHQHKLAVGIDLGTTNSLVATVRSGQTQVL
                     LDEQERALVPSVVHYAETEKTVGIEAFAKAALDPKNTVISAKRLIGRSLADVQQRYQN
                     LPYQFVASENGLPLIQTAQGNKSPIEVSADILSHLAQFAEKRLAGELSGVVITVPAYF
                     DDAQRQSTKDAARLAGLNVLRLLNEPTAAAIAYGLESGQEGVIAVYDLGGGTFDLSIL
                     RLRKGVFEVLATAGDTALGGDDFDHLLAEWIAEQANYKPQTASEQRELLTLATQTKVA
                     LSQAVEFEVKFANWTGVVSRSQFNELIQPLVKRSLMTCRRALKDAGVEAEEVREVVMV
                     GGSTRVPFVREQVGEFFGKTPLTSIDPDKVVAIGAAVQADILVGNKPDSDMLLLDVVP
                     LSLGIETMGGLVEKIIPRNTTIPVARAQEFTTGKDGQTAMTIHVLQGERELVEDCRSL
                     GKITLRGIPAMAAGVAQVRVTYQVDADGLLNVTAMEKSTKVQASIQIKPSYGLTDEEV
                     TQMIKSSMANAKEDMEARQLVEQRVEADRVIDSVVTALKEDGSAVLSVEEFQAIEAQL
                     ARLIELKEGTDRFAIQQGIKEVDLATQEFAARRMNLSIQKALAGKKMDDLA"
     gene            complement(42799..43140)
                     /locus_tag="PXE44_000227"
     CDS             complement(42799..43140)
                     /locus_tag="PXE44_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HDL5587775.1"
                     /translation="MKAILCQRAKQLRTNMTEAEKVLWQALRAKRFLAIKFKRQQVIG
                     NYIVDFVCFSAKLVIELDGSQHLDQQNYDNLRTHFLNGQGFRVLRFWNNQIHSDLEAV
                     LDTIFYEITRG"
BASE COUNT        13205 a         9057 c         8566 g        12314 t
ORIGIN      
        1 tattgaacaa gaacttgcag acttaggcat tacaaatgta aaagaaatcg tgcgtaaccc
       61 tagctatgag caactgtttg aagaagagat gaaatcggat cttaaaggtt atgaaaaagg
      121 cgtcctaaca aatttaggcg cagtagcggt agataccggc attttcacgg ggcgttcgcc
      181 gaaagataaa tatatcgtat tagatgaaac gacaaaagat accgtctggt ggacatctga
      241 tgcagctaaa aatgataata aaccgatgac tcaatctact tggcaaagtt taaaaacctt
      301 agtgaccgat gagttatcta ataagcgttt atttattatt gatgctttct gtggtgcaag
      361 ccctgagcat cgcatcaatg tgcgtattat tacccaagtt gcgtggcaag ctcactttgt
      421 gaaaaatatg tttattcgtc caacagaaga agaattaaaa gatttcaaac cggattttgt
      481 ggtaatgaac ggttcccaag taactaaccc gaactggaaa gagcaaggct taaattcaga
      541 aaactttgtg gcatttaata tcacagaagg tgttcagatc attggcggta cttggtacgg
      601 tggtgagatg aaaaaaggta tgttctctat gatgaactac ttcttaccat taaaaggtgt
      661 cgcttcaatg cactgttcag ccaatgtggg cgaagaaggt gatgtggcgg ttttctttgg
      721 tttatcagga accggtaaaa cgacactatc aaccgatccg aaacgcaaat tgattggtga
      781 cgatgaacac ggttgggacg aaaacggcgt atttaactat gaaggtggtt gctatgcgaa
      841 aaccatcaac ctttctattg aaaacgagcc ggatatttat cgtgcaatcc gccgtgatgc
      901 gttattagaa aacgtggttg tgcgtgaaga tggcactatt gattttgatg ataaatcaaa
      961 aacagaaaat acccgtgtga gctatccgat ttatcatatt gataatattg ccgtgccgtc
     1021 aaaagcagga cacgctaaaa aagtgatttt cttaacggca gatgcattcg gcgtattgcc
     1081 accggtgtct aaattaacgc ctgaacaaac taaatactac ttcttatcag gctttaccgc
     1141 aaaattagca ggtacagagc gtggtattac tgagccaacc ccaactttct ctgcttgttt
     1201 cggtgcggcg ttcttatcac tccacccgac acaatatgct gaagtgttag tgaaacgtat
     1261 ggaagcagcc ggtgcggaag cctatttagt gaataccggt tggaacggca caggtaaacg
     1321 tatttcgatt aaagatacac ggggtattat tgatgccatc ttagacggct caattgagaa
     1381 agcggaaatg agtacattac caatcttcaa tttagcaatt ccaactgcgt taccgggtgt
     1441 agaaacccac attttagacc ctcgtaacac ttatgaagac aaagcacaat ggcaagctaa
     1501 agcagaagat ttagctggca gatttgtgaa aaactttgaa aaatatgcga ccaacgagga
     1561 aggcaaagca ttaattgctg ctggtccgaa gttataagtt gtaattataa gttataatcg
     1621 ctaaaaaatt agtttaggac taaaccttga atgaggttta gtccttttgt ataaaggaga
     1681 agtacaataa tgacaacatt ccaattaagg ctgaaagcca aagatgagct taccccacac
     1741 ccaacagcac aatattggcg aaaatgccaa gtggaaggac tatttgatct gccgtttatg
     1801 gatttagtgt ttagagcggc acaagttcac cgtgaaaatt ttgatgctaa tgcgatccag
     1861 ctttcaacgt tactctcaat taaaacagga ggctgtccgg aagattgcga atattgcccc
     1921 caatctgccc gctatgatac gggaatagaa aagcaaacaa tgcttgatgt agagacgatt
     1981 gtagaaaaag ctaaaattgc caaagagcgt ggagcaagcc gcttttgtat gggggctgcg
     2041 tggcgaggac ctaggcctaa agatattaaa gtagtctcag aaatcatcaa tgcggtgaaa
     2101 tcattggggc tagaaacctg tggtactttc ggtatgcttg aagagggaat ggcagaagaa
     2161 ttaaagcaag cagggctgga ttactacaac cataatttag ataccgaccc tgaacgctat
     2221 aacaaaatta tccatacccg aaatcacgaa gatcgaatgg acactttagg taaagttcgc
     2281 aatgccggct tgaaagtttg ctgtggcggt attgtgggaa tgaacgaaac ccgccccgag
     2341 cgagcaggcc taattgcaag cctcgctaac ctcgatcctc aacctgaaag cgtaccgatt
     2401 aatcagttga ttaaagtaga aggtacaccg cttaccaatg ccgaagattt agactggaca
     2461 gaatttgttc gtaccattgc ggtagcaaga attactatgc ctcaaagctt tgtccgactt
     2521 tctgctggca gaactactat gccggaggca atgcaagccc tctgttttat ggcaggagca
     2581 aattctattt tctatggcga taaattactt actacagaaa atccggaaga agatcatgat
     2641 cgccaattaa tggataaatt agatttatat ccgctacaat atatagcctg agtgccaatg
     2701 attttcgcct gaatgaaaat taaagaatca atcgttattt tacgcaaatt tctaacataa
     2761 aataaccgct tgcaaaattc gttcttgtct tatttttaaa tttatctata atgtgaggcc
     2821 ttacgcaagc ttgcgtaatt cgatggggcg ttattggttc ctcgcaatgg tgttcagctt
     2881 acttgatcag gttcggaaga aagcagtctg ggttgaaatt tccgtgtgcc gagggaggct
     2941 ggtaatgccc cacccttttt tcagttctat caggagaacg ttatgctcca gatcaccccc
     3001 attccggcat taaaagataa ttacatttgg gcaatccgta acgggcaaga tgtgattatt
     3061 attgatccat cagaacatca gccagcatta aactttattg caaaaaatgc gttaaattta
     3121 accgctatct tgattaccca taatcaccac gaccatactg atggggtgag tggtattgtt
     3181 gagcaatatc cgaatatccc tgtttatggt ccgcaagaag tggctgaatt tgccaatgtg
     3241 attgtctcgc ctgaacaaca tatcagcctg tttggttatg atgtaaggat tattgaaagt
     3301 ggtgggcata ccgcagggca tatcagttat ttatttggct atgattatct gttttgtggc
     3361 gatgcgttat tctccggcgg ttgcgggcgg gttttcaccg gtgattacca agcccaattt
     3421 gatgcgttac aacgctttaa aaatctgcct aactcggtta aagcctaccc ggctcacgaa
     3481 tacaccaaga gcaatttgaa atttgcagaa gcggttctac caccaagctg tgtattatcg
     3541 gaatatcaag aacaggttga tcttttacgt tcgcaaaaca aaccgacttt gccaaccact
     3601 attggcttgg aaatgcaggt gaacccgttt atgcaagcgg tcgatttagc cgaatttatt
     3661 gcacttcgtc agcaaaaaga caatttttaa cctcatattt ctaattaagc attaaagcct
     3721 ctcactttaa tgctttttgg ttaaataaaa cctttgcaaa catcgcttgt caattccact
     3781 ctggcttatc ctatggtata gttacgacta tttttagctt aatttattat tttagtgagt
     3841 aaaattctgt gaacttcgat cctcaaaata tgcctaagca cgttgccatt attatggatg
     3901 gcaatggcag atgggctaag cagaaaggaa aattacgcat ttttggtcat cagaatggtg
     3961 taaaggcagt tcgttctgca gttagttttg cagcaaagca tcatatccaa gttttaaccc
     4021 tctacgcatt tagcagtgag aattggagca gaccagaaac tgaagtttca gctttaatgt
     4081 ctctgtttat gaaagcttta gattctgaag ttaaacaatt acataaaaat aatattcgct
     4141 taaagattat aggcgataaa tccgccttta gcgaaaattt acaaaaacgc attgccgaat
     4201 cggaaaaatt aaccgaaaac aatactgggc taatcttaaa cattgctgcc aactatggtg
     4261 gttactggga tattgttcaa gctgctcaac aactagcagt gcacgttaaa tctgaacaac
     4321 ttgctgtgga gcagattaca ccagcattgt ttcaacaaca tttagtgaca gaaaaccagc
     4381 cgcaagtgga tttattaatt cgaaccagtg gtgagcagcg gatcagcaat tttctattat
     4441 ggcaaattgc ttatgccgag ctgtttttta gcccggtttt atggcctgat tttaatgatg
     4501 aggcttttag tgatgcaatt tatgcttatc agcaacgtga acgccgattc ggtggttgtt
     4561 aatataaaaa gaataaggaa atacaatgct taaagaacgt gtactttcag cgattattat
     4621 gattattgcc gtgctggctg ccatattttg gctctcacca cttcctttaa ctttagtgtt
     4681 atcggcaatt gttgtggctg caatgtggga atgggctcaa tttgccggct ttaaacaccc
     4741 tgtgccacgt gcaattgtgg caatggtgac aatctgctta ttactttttg cgatcgttgc
     4801 gaatactgat tatatccgag ccacccgttt tattactgat gagactaccc ctatcctctt
     4861 tattggctgt atttggtggt taattgccct tgtcctagtg gcaacttacc ccaactcggc
     4921 aaaactgtgg gaaaaatctg ttgcagctaa attcctattt ggacttgcta ccttagtgcc
     4981 gtttttaatt ggcacgctcg cattgcgttt ccataattat gcatttgatc cataccaagg
     5041 aacttattta ttcctttacg tctgtttatt agtttgggga gctgattcag gtgcttattt
     5101 tgctggcaga gcctttggca aaagaaaact tgccccaaaa gtatctccgg gtaaatcgtg
     5161 ggaaggcgta attggtggcg tactcacctc tagtttgatt gcctttacct ttttacaact
     5221 tacaccggca aatgtattcg gcaaagcatt agccactacc ccatttattt tagtttcggt
     5281 cggtatggtt gctatttcag tcttaggcga tttggcagaa agtatgttta aacgccaagc
     5341 aggcattaaa gacagtagcc agttaattcc tgggcatgga ggtattttag atcgtattga
     5401 tagtttaacc gctgccgttc cgttttttgc tacattcttc ttttttgtac tatagaatat
     5461 gacgtccatc attgcatttt ttattttaat ttgcgtactg gtttttgtgc atgaatacgg
     5521 gcacttttgg gctgctcgca aatgcggggt aaaggtgatc cgtttttcga tcggatttgg
     5581 taaagtctta tttaagaaaa aagataaaca aggaaccgaa tttgtttttt cacttattcc
     5641 tctgggcggc tatgtacaaa tgtggaatga tgaaacggat atcaacgctc cagctcaaca
     5701 agctcttaac acaaaatctg tattgcaacg tgcatttatt attatagccg gtcctgctgc
     5761 aaattttatt tttgcgattt tggcatactg ggtcgttttt attgccggta ttccaactgt
     5821 taaacctgtt attggggaaa ttttaccaaa cactatcgcc tcccaagcaa gaattcctac
     5881 cgaattggaa attacaaaaa tcggtcatca atctatccaa gattgggaaa gtgcatcttt
     5941 agcactagtt ggttttgtgg gcaataaaaa tgtaccgtta gagggaaata tcaacggcaa
     6001 ctcaacacaa aaatatgaac tcgatttatc taactggagt attgactcag ccaaagaaaa
     6061 cccattaacc gcattaggta ttcgccccaa aagtgcaaaa gtcaccgctg aaattaaaca
     6121 agttgtcgaa aattctcctg caagccaagt gggtattcaa gcaggtgatc gtattcttca
     6181 aattaaccag cagccattta attggttcaa tttagttgaa ctggtacaag caggtaaacc
     6241 tattgaactt aaaattgaac aacgaggtca gattaaagac cttgtcgtcc aaccagaaaa
     6301 aaaagacgaa cgctatatta tcggggtaat accaagttat gagcctcttg cagataaata
     6361 tcgtaccgaa ttaaaatatg atattcttac cgcattttat aaaagtatcg aaaaagtgtg
     6421 gtctcttacc caaaccatac tccaatttat cggtaattta ataagcggtg atttgtccat
     6481 taaaaattta ggtgggccaa tttcaatggc aaaaggagcc ggtgcaacag ctgaaatcgg
     6541 cttagtttat taccttagtt ttatggctct aatcagtgtc aatttggggg taatgaactt
     6601 atttccactt ttacccttag atggtgggca acttgtttta ttggctgttg aagcaattcg
     6661 tggaaaagca ttatcagaaa aaatacaact caaattccaa caaattggat ttgcattcgt
     6721 gctgagttta atgttattcg cctttgccaa cgatataatt cacttttaac aagcggttaa
     6781 aaaaggtgac ttttttgcaa aaagcaccct gacatcaaaa tccataatat ggatttaaac
     6841 tatattctta ataattttgc aaaacgatca taggataatt tcaatgaaaa aattattact
     6901 ttcctctctt ttaattgcaa acggtgtagt tgctgcacct ttcgtagtaa aagacattcg
     6961 tgttgaaggt gtgcaaccta caacaggcga atctatcatt tcatctattc cggttagagt
     7021 aggtcaaaca gcaacggata ctgacgtatc taatgtcgtc cgccatctat tcagccaaca
     7081 acgttttgca gatgttcgtg caacacgaga aggcaatacg cttgtcatca aagtcgctga
     7141 aaaaccgatt attggcaagg tagagatcga aggtaaccaa gcgattccta aagatgcctt
     7201 agagcaaaat ttaaaggcaa acttaattaa ccaaggtgaa atttttgatg ctgccaagct
     7261 tgaagctttt aaagaaggac taatcgaaca ttatcactct accggccgtt atgaagccaa
     7321 aattgacaca tctgtttcga ataacaccga aggtggcgtt aatattaagt tagcgattaa
     7381 tgaaggtgaa gttgctaaag ccaaaacaat taagtttgaa ggtaataatg ccttctctga
     7441 taaaaaatta ttagaaaact tagatattca acctgatgtt tcttggtgga atatttttga
     7501 aagtagcaaa ttcgaacaaa ccgcctatca aaaagactta gaaacattaa gagattttta
     7561 tttaaatcgt ggctatgcaa aacttgctat ccaagatacc gatgttcaat tcaatgataa
     7621 taaaacggaa gttgcattga cctataaatt agatgaaggg tcacagtacc atgtcagtga
     7681 aatgcgcatt atcggtaata cagccaatat ggatagccag ctcaacgccc tactaaaaga
     7741 attcaagcct ggtcaattat tccgtaaatc agaccttaca caaattgaag aagagattaa
     7801 atctatttta ggggacgcag gttatggttc agctaaagta gatatttatc ctcaatttaa
     7861 tgaagaaaat aaaaccgtaa aaatcagttt tgttgtggat gccggtcgtc gtatttatgt
     7921 tcgtaaaatc cgttttgaag gtaatggtgt cactgcagat tcaacattac gtcgtgaaat
     7981 gcgccaacaa gaaggtgctt ggctttccac aaacaaagct tctctaggta aagctcgttt
     8041 agaaagaacc ggtttctatg aaagtgttga tatgtcaatg ccaaatgtag aaggtacagc
     8101 cgatcaagtt gatgtagtgt ataaaattaa agaacgcaat accggtagta ttaactttgg
     8161 tatcgggtac ggcacgaaaa acggtatcag ctatcaagca ggaattaaac aagataattt
     8221 cttaggtatg gggtcaacta tcagtctaaa tggtacccgt aataactacg gtaccagcgt
     8281 taacctaggc tataccgaac cttactttac taaagatggt gtaagtttag gcggtaatat
     8341 tttctatgaa gattatgata actcagacaa tgattcaatg gcttcatata aacgccaaac
     8401 ctatggtatt aacggcacat taggcttccc ggtaaatgaa aataactcat actacttagg
     8461 tttaggctat acgcacgata aaattaaaaa tgcagcacgt gagtacactc gtgagaaata
     8521 tgtaaaatca atgaactttg agttcgatcc agctgccgat tattacaaaa aaatcaaagc
     8581 tgatgatttt gatttttctc tcggttggaa ctacaacagc ttgaatagag gctatttccc
     8641 gactgaagga actaccgcaa acattggcgg taaaatcaca attccaggct ctgataataa
     8701 atattaccga gtcagtgcag actttagaaa ttattacccg ttaaaccgtg agcacaaatg
     8761 ggtaatttca acgaaagcta gcattgctta tactaatgga tttggtggta aagaagttcc
     8821 gttctatcag ctttatactg ccggaggtat tggtacacta agaggtttct cttacggtgc
     8881 agtagggcct caagcgattt actataatac aaaatccggc aaatttgata gcatgaataa
     8941 tgatattatc ggtggtaatg cgttagctgc ggcaagcttg gaattaatca caccaacgcc
     9001 atttgtgagc gaaaaatatc agcacaatgt aagaacctct ctctttgttg atgctgcaag
     9061 tgcatggaat acaaaatgga agaaagatga atatacgaac ttgcctgact atggtgattt
     9121 caaacgtttc cgtgcttccg ccggtattgc attccaatgg atgtctccaa tcgggccatt
     9181 atcattctct tatgcgaaac caattcgtaa atatgataat gatgatattg aacaattcca
     9241 gttcaacatt ggtagttcat tctaattaac cacaagcggt taataaatgt aaaaaatttg
     9301 caacttagcc gcttatttca aaactgttca tacaaggaaa acaaatgaaa aaattattta
     9361 aaatgactgg cttagccgta gctttagcct ctacaaccgg aatcgcgaat gctgcagata
     9421 ctattggttt cgtagatcca agctatgtat tacaaaacca ccctgtatta ttagatgctt
     9481 cggcaaaatt tgataaattc atgaaagaaa gccaagaaaa atttgctgct gatgaaaaaa
     9541 aattagcaga tgaaaataaa accttaacgg ctgagcgcaa taaaattaat gaagatgcgc
     9601 aaaaattaca aaatgaacaa aaaaatgtag aagcttctat caagaaaaaa gtggcagcat
     9661 tagagaaaga tgcgcctcgc ttacgttcaa aagagattca agcacgtcaa aatgcgattc
     9721 aaaaagaagc aaacgcattc caagctaaag taacagcaat tcaaaaacgt gaagctgatt
     9781 ttgcaaaaaa agcagaagcc ttccagaaac gtgcagatga attccaaaaa cgtattgaac
     9841 aagcgaataa agaaaatggt ggcattaacc cacaagaagc gcaaaaacaa gcggttgatg
     9901 aagtaaatgc aaccattaaa gagatcgcaa aatcgaaagg ttacacttta gtattacaac
     9961 cacaagtagc actttatgct gaagatgaag gtaaagatat tactgaagca gtacttgatg
    10021 ctattgttaa aaaacatccg gaaattaaaa tagaaaaacc tgcaactgaa gctcaaccag
    10081 ctacagaaaa agaaaccaaa gctgaagaaa ctaaaccaga agaagcgaaa aaataatggc
    10141 aaatttccgt ttaattgatt tagcggagca tatcggcggt actcttaggg gtaacgccga
    10201 tttggctatt caaagtattg ctgctttaga taaagccgac agctcgcaaa ttacctttat
    10261 ctcaaatgca aaatatcggg aaaatttgac cgcttgcaat gctggagcta ttatcgtcag
    10321 cccggcagat gtagaatttt gccgagcaga tcaaaatctt atcatcgttg ccaatccgta
    10381 tgttgcttat gctcaactgg ctcaatatat ggattctact ccgaaagcag cgagcgaaat
    10441 ccacccaagt gcggtgattt cacctgaagc taaactgggt aataacgtat cggtaggggc
    10501 aaatgccgtg attgaaagtg gcgtagaatt gggcgatgat gccgtgatcg gtgcaggctg
    10561 ttttgtggga aaaaacagta aaatcggggc aagaacccaa ctttgggcaa atgtttcggt
    10621 gtaccacaat gtacaaatcg gagctgattg cttgattcaa tcctcaacgg ttatcgggag
    10681 tgatgggttt ggctatgcga atgacaaagg gcaatggatt aaaatcccac aaaccggtgg
    10741 tgtgattatc ggtaatcgtg ttgaaattgg tgcttgcact tgtattgacc gaggtgcatt
    10801 agatccaacc gtaatcgaag ataatgtgat tattgataac ctttgccaaa tcgcccataa
    10861 tgtacacatt ggttttggca ctgccgttgc cggcggcgtc attatggcag gcagtctaaa
    10921 agttggtcgc ttttgccaaa ttggtggagc aagtgttatc aacggtcata tggaaatctg
    10981 tgatggtgcc ataattaccg gaatgggtat ggttatgcgc ccaattacag aaaaaggaat
    11041 ttactcatca ggtattccat tacaaaccaa taaagaatgg cgaaaaaccg cagcacttgt
    11101 aatgaatatt gatgaaatga acaaacgctt aaaatcgtta gaaaaacgtt ttaacgatta
    11161 attttattag aggtcttaaa gtgacagaaa tgacaactga aaatcgtgag ccaaatatta
    11221 ttgaagtaac cgaaattatg gatatgttac cgcatcgcta ccctttcctg ttagttgatc
    11281 gcgtaaccga ttatgaagaa gggaaatggt taaaagccat taaaaatgta accttcaatg
    11341 aaccttgttt taccgggcat tttcctggcg caccaatctt cccaggagta ttgattctgg
    11401 aagcaatggc tcaagcaact ggcgtattag ccgttgccac ttatggtaag ttaagtgaag
    11461 acgaactcta ctattttgcc gcaattgaca atgctcgctt taagcgaccg gttgtgccgg
    11521 gcgatcaact tcaacttgag gttgaatttc ttaaagaagt acgtggtatt actaaattta
    11581 ccggtaaagc atacgttgat ggcaaattag tctgtgaagc agatttaatg tgcgctcgcc
    11641 gtaaataatt cgatataagc ccaaactaga gggaacaaac tatgcgtcta attgattcaa
    11701 cagcaaaaat tagcccgctt gccatcattg aagaaggtgc tgaaattggc gctcacgttg
    11761 aaattggtcc attttcagtc atcggtaaag atgtcaaaat cggggctaga acgaaaattc
    11821 actcaaatgt tgtcattaac ggcatgactg aaattggtga agataaccat attttccaat
    11881 ttgccagcat tggtgaaatc aaccaagatc taaaatacca aggtgaacca accaaagtgg
    11941 tgatcggtaa tcgtaaccgt attcgtgaaa gtgttactat tcaccgaggt acggtacaag
    12001 gcggtggcgt gacgaaaatc ggtgatgata acttatttat gatcaacacc cacatcgcac
    12061 acgactgctc tatcggtaac cgttgtatta tcgccaataa tggtacgcta gcaggtcacg
    12121 ttaccctaga tgatttcgtg attgtgggag gaatgtccgc cattcaccaa ttcgttgtga
    12181 ttggttcaca cgttatgctt ggaggcggtt caatggtcag ccaagacgta ccgccttatg
    12241 tcatggcaca aggtaaccac gcacgcccat ttggtattaa ctttgaaggg ttaaaacgtc
    12301 gtggctttga taaaccaact atgcacgcta ttcgcaaagt ctataaactg atttactcca
    12361 gcggaaaaac attggaagag tgcctagtag aaattgagca aattgcagca acagaacctg
    12421 cgattgctat ttttaaacaa ttcttcaaac gttctacccg tggtattatt cgttaataaa
    12481 cgaaaccccg aaatcaattc ggggtttgtt ttatcaagcg accgtcgaac ctttcaacaa
    12541 ttttcgaatc caagctcgat ttgggctaag gttatgccaa atatcctcac tgattggcac
    12601 aggctcattc acaatcaggc ttgctaacaa ttcccctaga agtggtgcag tcgtcaaacc
    12661 tcgagaagac aggccgttta ccatatacag attggtaaaa atttcagcat tttctaccgc
    12721 ttgtttccgt ctcaactggt tgtataaatt ggcatattgg cttttttgct gctcaaaatg
    12781 atgcactgcc ccaaccatcg gtacacgatc acgaaacgcc gcacgaatac cgactttggc
    12841 tttattttca gataaatcaa ttccttgcgt ccactcacaa acggttacat tttgctgaag
    12901 ttttgcaaca ttctcccaat gctccgataa actaaactcg gtttctgcat tatcccgaat
    12961 atggcttgct ccaatgcaat gcgtgccata ggctgacatt ggcgttaaat aaccatcata
    13021 acaaagcaca cacttcagtt tttgtaattc gcttgtagtc ggaatttggc tcacttgccc
    13081 tcgtaccgga tacaacggaa tgccgttcgc ttggctaaat tcattcaacg tatgtccatt
    13141 tgccagcaca accgcttgat gtcgaaccgt ttctcccctc gtctgccatt gccacattcc
    13201 attagaaaag gttaaatgct caactttgtg attcagtaca atcttcaacc ctttctgctc
    13261 cagccaagta aagccatttc taacaagttg taccggtgaa agccaaccag cttgcggaat
    13321 aaatgccccg ctgtttggca cgcttaaacc taatttttca cttaattctg cctgtgaaca
    13381 agattttaat aaggtgtcat cagccgtctg ctccaccatt ttttccagtt ttttagccgt
    13441 tttctcgtca taggcgtaaa gcactacccc gcttaaatta tgctcaaact caatgttgga
    13501 tttcagttgt tgcaagcgtt gcaatgcata atcaaaacta tggatataaa actgtacatt
    13561 tcgctcgtca tcatcgctta actgtgggta aatcgcccct tgtaaatttc ctgaagcatt
    13621 tattgccaag gtttcatctt tgcaatacag cgtgacttgc ttgccttttt ctaacagcga
    13681 taacgcaacg aagaaactcg ctactccgcc accaataatt gcaatgtcat cagcggtttc
    13741 atttttggca taaaaatagg ggtaattagg ctcggaaact tggctatcta acggtttctc
    13801 gccccaaagc atttctcgtt ttttgccaaa tcctttgcgt tttttcactg caaaacctgc
    13861 ggcttccagc cctcgtctga ccatacttga agcggtaaaa gtagcaaaac tgccgccatt
    13921 ggcacttagt cgaaacattt gcagatataa ctgttcgttc cacatttctg ggtttttatc
    13981 aggcgaaaag ccatctaaaa accaagcgtc aatcgtgccg ctataaagat cgcctaattg
    14041 gggtaagttt tcagcaatct caccgaacca taaatctaaa taaacctgct caaaatgata
    14101 acgttggcaa cctatttggc gaggctgcca acaagcggtt aaattttcag ataatgttgc
    14161 aaattgcgga tagcgttggt gaatatcagc taattggctg gccgtcagag gaaatttctc
    14221 aaatgagata aaatagagtc gttttagttt actttgcgga aatgctgccc gaaactgttg
    14281 gaatttgtca gcaacggcta aaaaattcaa gcccgtgcca aagccggttt ccgcaataac
    14341 aaaagcctct ttcgggtgag attgccactt ttcccacagt tggttacctt cttgaaacac
    14401 atagtaactt tccgccaaac catcttgggt agaaaaataa atatcatcaa attgctccga
    14461 caccggtgta tctgcatcat taaatgtaag tgtcgcaaaa gtcagtttac ccatcatatc
    14521 cccttaaaaa caaaacacgg aaaaccccaa gattttccgt gtttcaacca gttcaatagt
    14581 accaatgtta gaaaatgttg ctggcaaaaa taacgatcat accaagtgcg cctacaaccg
    14641 ttagcattga ataaccaaaa gtacccattt tacaatcctc attgttaatt atgattaaaa
    14701 cggctttatt ctagcataat ttccatcgct ttgaatgcct atgcttgaat aattttgcca
    14761 aaacaagcgg tcaatttttg taaatctttt acgcctttgg cactttccac gccagaattt
    14821 aaatctaacc cgacacagcc ttgagcaagg gcttgctcaa tattctcagt gccaatacca
    14881 cctgccaata acgctttttg ttttaaggtt tctggaatca acgcccaatt aaaggttttg
    14941 cctgtgccgc cttgctgatt gcctactttg ctgtctaata cataacgagc aatctgtgga
    15001 ttatcgttaa attctagatt ttcaacctca acatcaaccg ataaggcttt ccagatttga
    15061 gttttactac ctaatttctc tgccagttct gcaatgtatt gctcatcttc cgagccgtgc
    15121 aattgaactg caaataactc aagctgtttt gccatttttt ccacaaattc gaccgcttga
    15181 ttttggaaca cccccacata acgcaacggt gcattcacca ccaattcttg ggcttggcga
    15241 agtgaaagct ggcgaggcga gccttcggca aaaattaaac cgccatataa tgccccttgc
    15301 tcataaaccg ctttcacatc ttgcacacgg gttaagccac acactttgtt ttcaccaaaa
    15361 atcaccgccc gcaccgcatt attgagatcg gcacttccca ttaaactgct gccgattaaa
    15421 aaagcatgaa catacggctt aatgtttttc acttgctggt gatcgttaat acccgattct
    15481 gaaatcaaac agacatcagc cgaaatttgg gcttggtatt tttccaccaa acgcacaatg
    15541 cggttcatat caatcgtgag cgtgtgcaaa tcacggttat taacgccaat taccttcgcc
    15601 cctaaggcta aggcacgttc aaattccgcc tccgtgctgg tttcagtcaa aacgcccatt
    15661 cctaactcgt gagccagtgt ggcaagttca cggtaggttt catcatttac caccgataac
    15721 atcaataaaa tcgcatcagc attataaaaa cgggcaaggt aaacctggta tgccgaaatc
    15781 ataaaatctt tacacaaaat cggctgtgtg gtttggcgtt tgacttgatc gatataacgg
    15841 aaatcgcctt gaaaatattg ctcatcggtt aaaaccgaaa tcgccgcagc gtaattttta
    15901 tacacttggg caatactgtc taaatcgaac tcagcacgaa ttaacccttt tgacggtgaa
    15961 gcttttttac attctaaaat ataagctggt tcttggtgtg tgcctttggc aagagccgca
    16021 tagaaatcat ggtcgctttt tacaattttg tgctgaaatt cacttaacgg aaaggcttgc
    16081 tctttttgtg ccacccaaat ggctttatct tgtacgatct tttgtaaaat cgtcggttgg
    16141 tttggttgag cgttatcttg aacgatgttt tgcatataaa ttcctagagc gaaatcggtt
    16201 tgaatggtaa aatctgccct tatttttacc cgaaaaagtc tcatttcaca agcaaaacta
    16261 agatgaaatc acaagttata cagcatttat cttatttaga aatcgccctg agagttaatc
    16321 aactttggga ggcgacacca ccaagccctg aagccttagc caatgagcaa cctttctgcg
    16381 tggaaacctt aaccccaacc caatggctac aatggatttt tattcccaga atgcgggcca
    16441 ttttagatgc aaatgccgat ttacctcgca attttgcaat caccccttat ttagaagagg
    16501 cattgaaaaa cgaaggttat ttacaagcga tttgtgaacc gctattaggt ttggaaaagt
    16561 tgttgaaaga ttaacttctc tctcagttct tttattaatc tgacaaattc gttgatagtg
    16621 ccagcgtcca cgctggtact aattttaacg gttaattaat caaaatttgg cacaagcgag
    16681 gacgcttgcg ccatcataga gatttgataa attatactta atgtattatg accttagata
    16741 ttttatacca agacaactcg cttatcgcca tcaacaaacc tgccggagtg ttggtgcatc
    16801 gttcttggtt agataaacac gaaacggtgt ttgcaatgca aaccctgagg gatcaaatcg
    16861 ggcaacacgt ttttccgatt caccgccttg accgcccaac ctcaggtgta ttgctgtttg
    16921 ctctcaacag cgaaaccgcc cgaataatga gcgagcagtt tgaacaacac ctagtacaaa
    16981 aaacttattt ggcggtggtg cgtggctatt tacaaggttc ggcacaaatt gattatccgc
    17041 tcaaagtgat tttagataaa attgcggata aattttctca ggaaaaagag gctcaagcgg
    17101 cgattactga ttataaaggc ttagcaacgg ttgaaatgcc ttaccctgtc ggcaagcacc
    17161 aaacctctcg ttatagtttg gttgaactta gccctaaaac cgggcggaaa catcagcttc
    17221 gccgccacct gaaacacctg ttccacccga ttatgggaga cagcaaatat ggcgacttac
    17281 accaaaaccg tgctttagtt gaaaaatcag gcgtaagtcg tttaatgtta cacgcccata
    17341 aattgcaatt tcagcacccg aaaaatttgc aaaaaataga gataattgca tcgcttgatg
    17401 agcaatggca acgcctcttt gcattttttg actggaactt tactcaatat tattagacaa
    17461 aaaagaggat tgaatggata attcactttt atcattgaca cacgagcaac aacaagccgc
    17521 agtagaagaa attcaaaaac tgatggcaca aggcatcagt gccggagagg caattcaaat
    17581 tattgctaat caattacgtg acaaatatca aatattggag aaatcaaaca atgaataaat
    17641 tatggacagt gcttgcccta acaataactt cccactatag ctttgctgcc gctgaaaaaa
    17701 tagtcactaa agatttaccg gcagaaggaa aacctgcagt cgtgctacag gtttttgatg
    17761 aaagtgaaaa aattgcaaaa cctattaagg gaaataagct ctttattagc aaaaaacaaa
    17821 caaggctttg ctggcacagt attaatcttc ctgtacaaaa taaagttact attgcagaag
    17881 ctttctatgc ccctgctgca atgaaattag cttctcccgg ctctaaaatt aattcttctg
    17941 cagacaagaa aaatcatact atcattactg aaataaacct tgcaaataat caagtattaa
    18001 atcgttgctg gggttttgat aaaactgacc ctgtcggtaa atacaaaatg gaaattcaaa
    18061 ttaacgatca cattttcaaa ggtttagaat ttgaaattgt aaaataacct aaaagccaca
    18121 gagtaacacc ctgtggcttt ttgctaaatc attgttttta aattggttaa attgtgcagc
    18181 aaataatctt tactcttcac aaaggctctc gcctgttgcc agttgctaaa ttgttgctga
    18241 gtgcattgat taaaacgtac cagcacttcc aaactttcat tgccgacaca aataaaatca
    18301 gctaataatt gggttaattt ttcactgttt tcaccatcca ttgcagttgc aattttctgc
    18361 tcccatttaa ccgcttgtcc gagcaaataa ccccgctggg ctttgcttaa attactcaac
    18421 cacatcccat ccgcttttgg cattgcctca accaaagtaa ataagtcgcc tagatttcct
    18481 tgcttgattt caaactccaa ttcacaaatc ggctcttcac caaaatgatt tttaatgatg
    18541 ccctgatcta acgctacctc aatgtcagac atccccactt gcaccaacca agttttacga
    18601 gtgaaatcgg tgctgaatat cgcttgcaag ctttctgcaa tctgctctga attttcaaac
    18661 ggcaactggt atttttccac taatgtttga aaatccggct ggctattggg taaagctaaa
    18721 ttatattccg gacgaatatg caaaccgcct acaatttccc cttgtgtttt taacgtgatt
    18781 tcatattgtt gattcacaga acgcacccgc agccccattt tttgcgtggc aaaaaattgc
    18841 tcgggcgtat cataataggt gttgcctaac aattccgttt gatgagcaag cacagggaat
    18901 ggcgaggttt gattcaagta gttttccact aacgccacat tctgctgttc caacattatt
    18961 ttcagttcga tttctgcttg tttttggttt tccatttcac acgcccctgc aacgttgtat
    19021 aatcaagcgg ttattttatc aataaatttt gcaaaatgtt ttcaaaaatt caccataaac
    19081 tgaataactt tgtccagcgt ggactcgaca accatttacg cctcgccgtt accggcttaa
    19141 gccgtagtgg caaaacggca tttattacca gcttggttga tcagcttttg catatcaaca
    19201 aaggcggtaa ttcgcattta aacctgtttg cgcctgcacg ccacgggcaa attctatcgg
    19261 taaaacgcgt tgagcaaggg gatttaacta ttccccgctt tgaatatgat aaaaaccgcc
    19321 aaagccttga aaatcaacca cctcaatggc ctgaatccac cactggcatt agcgaaattc
    19381 gccttgcgat tcgctaccag cggcaacatt ctctgctacg tcatattaaa gaaaccggca
    19441 cgctttattt ggatattttc gattacccgg gcgaatggct gttagattta cccctgctct
    19501 ccctttccta taaagaatgg tcgcaaacgc aacaaaccat acataaagat gagcgtgcag
    19561 agcttgccac accttggtta caagcggtta aaaaactcga tctttttgca aaaaccgatg
    19621 aaaatttact ggcagaattc agccaacaat acaccgatta tttgctcgcc tgcaaagctc
    19681 aaggaatgca atatattcag ccggggcgat ttgtgttgcc aactgaaaat gccaaaggtg
    19741 caccggtatt ccaatttttt ccgttattag atttaagcga agctgagtgg gaaaagttgg
    19801 aacaaagcga cagaaacagt attttccata ccctcaaaaa acgctatgag cattaccaga
    19861 aaaaaatcgt caaaccgttt tatgaagatt acttctctca atttgaccgc caagtgattt
    19921 tggcagactg cctcacaccg ttaaattatg gctataacga atttatggaa atgaaattag
    19981 ggttacagca actgtttaaa catttccatt atggcaatcg ttcgctattt caccgtctgt
    20041 tttcgtccaa cattgataaa ttgctattta tcgcaacaaa ggcagatcac attactagcg
    20101 accaagtgcc gaatttggaa agcctaatgc gacaattagt gcaagaaggg ggaagacacg
    20161 cagaatttga tggtatcgaa accggctatt tagccatttc ctcgattcaa gcaaccgatg
    20221 cggtgaaagt gactcagcaa gataaaatca ttaaagcgat aagagggatc cgttccagcg
    20281 atagaaaaca agtgacactc taccctggca gcgtgccaaa tcgcataccc gataaaaatt
    20341 actggcaatt taaccgtttt gaattcgacc aattcgagcc caagcaactt gattatagcc
    20401 aagcgattcc acacttcaga atggattcgg tgctacaatt tttattggcg gatatgtttg
    20461 agtaaaatct tgttgcatat ttccattaaa ggtataaaat tttatacatt atgaatactg
    20521 taatttccca acgaaaacca attcactggg ttggatcaac cttagatgat attcgtgaat
    20581 ttccggataa cattaaaaga gaactcggtt ttgatcttga tttagttcag caaggcttat
    20641 taccgaggga ttttaaagcg atggcaaatt tgggttcagg cgtaatggaa attcgagtaa
    20701 aagatccctc aggggctttt cgcttagtgt atttagctaa atttaaagat gcaatttact
    20761 gtttacacgc tttccaaaag aaaacgcaaa aaacagcccc taaagacatt gctgtcatta
    20821 aagcacgcta tgctgccgta aaggagttag aaaatgaata atattgatac taaaattcgc
    20881 catactacag aagctcaggg taatgttttt gaagatcttg gtttccctat tgatgaagct
    20941 caaaaattga aatctgccag tcagcaactg attgaaacta aactgatgct aatgaatgaa
    21001 atgagcaact ggattgataa aaataatctc aaacaatcag aagcggcttc tattctaggt
    21061 gttagccgcc ctcgaatttc tgatatggtc aatggcaagt tagaaaagtt taccctagat
    21121 accttagtca cttttgttgc aaaaaccggc aaaacaatcc aattgcaatt atcctgatat
    21181 ttgcaaaatt ttactcaaat tcaaccgctt gttgctttta tcattcacta acaaggttat
    21241 ttcaagtaaa attgtgacta attttttatt aataaacaca aattatggca aaaaaaccta
    21301 aagttgcatc aaatacgatt gcgttaaata aacgtgccag acacgaatat ttcattgaag
    21361 atgaaattga agccggctta gagctacagg gctgggaagt caaagcattg cgtgccggaa
    21421 aaggcaatat cagcgatagc tatgtcatct ttcgccacgg cgaagccttc ttattcgggg
    21481 caatgattac gcctttgaat atggcatcaa cccatattgt cagcgacccg acccgcaccc
    21541 gcaaacttct actgaataaa cgtgagttag actcactgtt cggtaaagtt aatcgtgaag
    21601 gttataccat tgttgccacc tcaatgtatt ggaaaaatgc gtgggtgaaa gtaaaaatcg
    21661 gcttagccaa agggaaaaaa ctgcacgata aacgtgaaga tattaaagac cgtgaatggc
    21721 aagtggctaa acagcgtatt atgaaaaacg ccaatcgtgg ctaaatgaat aattaccctc
    21781 tccccttgtg ggagagggac agaaaaattg cgttcagcaa tttttcaggg aaagggggat
    21841 ttgtaaaagt tcaaggaaaa acaacgcttg ccctctctct gaaaaatatt tctgaacgaa
    21901 atatttttcg gtctctctcc cataaaggga gagagtgaaa cacaaattta aactaaggaa
    21961 catacaatga tcgaccaaaa cctacttcgt accaatttag aagacgttgc tacagctctt
    22021 aaaatcaaac gtaattttat tttagatgta gagcgagtaa aaacccttga agaacagcgt
    22081 aaaacgctac aagttaaaac cgaaacctta caagcagaac gcaacactcg ctccaaaaac
    22141 atcggtgcgg caaaagctcg tggcgaagat atttcagctt tattagccga agtagattca
    22201 atgggcaacg aattagacac ggcaaaagta gaattggata aagtacaggc agaaatccgt
    22261 gagttgttgc tttctgtgcc aaacttaccg gcagatgaag taccactcgg taaagacgat
    22321 agcgaaaact tagaagtatt acgttggggc gaaccacgtc aattcgactt tgaagttcaa
    22381 gatcacgtta cccttggcga aaacttaaac ggtttagatt ttgctgccgg tgtaaaatta
    22441 accgccagcc gttttgtggt aatgaaaggt aaacttgccc gcttacaccg tgcattatct
    22501 caatttatgt tggatttaca cactgaacaa cacggctatg tggaaaccaa tgtgccattt
    22561 ttagtcaatc acgacactct atacggcaca ggtcagctac caaaatttgg cgaagattta
    22621 ttccatactc aaccattaac agggcaagat ccaaacgagg tgcaacgccc attcggtttg
    22681 attccaaccg ctgaagtgcc agtaacgaac ttagtgcgag atgaaatcat tgatgaagac
    22741 agcttaccac tgcgttatac cgcccacacc ccttgtttcc gtgcagaagc cggttcttat
    22801 gggcgtgata ctcgtggttt aattcgtatg caccaatttg aaaaagtgga aatggtgcaa
    22861 attgtcgccc ctgaaaaatc aatggaagcc ttagaagaat taaccggtca cgcagaaaaa
    22921 gtcttacagc ttttaggctt gccataccgc aaagtgctac tctgcactgg tgatatgggc
    22981 tttggtgcag caaaaaccta cgacttagaa gtttggctac cggcacaaaa cacttaccgt
    23041 gagatttctt cttgctcaaa tatgtgggat ttccaagccc gccgaatgtc tgcacgttgt
    23101 aaagctaaag gcgacaagaa aactcgcttg gttcacacct taaacggctc aggtttagcg
    23161 gttggtcgca cactggtcgc tgtgttagaa aactaccaaa atgccgatgg ctctattact
    23221 gtgcctgagg tgttaagacc ttatatgggt ggtttggaag taattaccgc ttaagattaa
    23281 taaatgaaac caatctcaga aatggggttg gtttttttaa taaatcttat ccctaatctt
    23341 tttcaggata attcataaaa atttcagtaa ctactcggcg gttttgcact gtatctaaac
    23401 gaataggatt tttcattctc cctaaacgaa atgcaataat ggtgactttt ttcggttcat
    23461 ctattttatc taattgatag aataccatta tttcccgttg ataaaccgct cgctttctga
    23521 aacgtaaatc cttgcgaaat gcgacacctg cttttggata aaactgtaaa ccattgatga
    23581 tattcgtttg agtatgctcc gaaaataatc catagaaaat aaactctaat agattatttt
    23641 taaaatcaac actaaccatc taactgctcc caagcagtta aatctcctcg tgataaacgt
    23701 tcagcaatat cagcataaaa ttgtttcggt acttcccttt ttacttggta ttgagctgat
    23761 tgttcttcta ataaatgttc ttctatcatc ttgatttctt ctgttgtcca ctcttcatct
    23821 tctttatgta caacaaaatc ggtatctaca atcacaatag acatacctag ctccttcaag
    23881 tactggttat atattctgta ttttgcctgt tctaaattca gctgtctagc ttttctgtta
    23941 ctcacaattt gcaaattttt catcaaattc caccgcttgc cattcaaaaa aaggcgtttt
    24001 tgcggtatcc tatcggcaat tttttcagtt aaatttaaag gttaaatttt atgcaacagc
    24061 agtataaccc aagtgagatt gaacctaagg ttcagcgtta ttgggcagaa aataaagttt
    24121 ttaaagcagt taaagacgtt accaaagaga aatactattg cctttcaatg ttgccttatc
    24181 cgtcaggtaa gctacatatg gggcacgttc gtaactacac cattggtgat gtagtgtctc
    24241 gttatcagcg tatgatcggt aaaaatgtat tacagccgat gggttgggac gctttcggtt
    24301 taccggcgga aggtgctgcg gtgaaaaata ataccgcacc ggcaaaatgg acttacgaaa
    24361 acatcgaata tatgaaaggt cagcttaaaa tgttgggctt ctcttatgac tgggatcgtg
    24421 aagtcaccac ttgccgtccg gaatactaca aatgggagca atggttcttc acagaacttt
    24481 ataaaaaagg cttagtgtat aaaaaaacct caaccgtaaa ctggtgtccg aatgatgaaa
    24541 ctgtgcttgc caacgagcaa gttcacgaag gttgttgctg gcgttgtgac accccagtgg
    24601 aacagcgtga aattccacaa tggtttatca aaatcactga ctatgctgaa gaattactca
    24661 atcagttaga taatctccca cagtggccgg atcaagtaaa aaccatgcaa cgcaactgga
    24721 tcggtcgttc cgaaggggta gaaatcacct ttaaaattgc cggttcaaac gtagaattac
    24781 cggtttacac tacccgtccg gataccttct tcggcgtaag ctatgtggcg attgctgcgg
    24841 ctcacccatt agcagaatta gctgcacaaa ataacccggc attggctgaa tttatccgtg
    24901 aagcgaaaaa caccaaagtg gcagaagcag aattagccac catggagaaa aaaggaatgg
    24961 caaccggctt gttcgccatt cacccgttaa caggaaaaga agtaccggtt tgggttgcta
    25021 actttgtgtt aatgcactac ggtacaggtg cggtaatggc ggttccggct cacgatgagc
    25081 gtgactttga atttgcacaa aaatacgatt tgcaaattaa tcaggtaatt caaccgcttg
    25141 atggctcaga gtgggatttc agcaaagccg cttacaccga acacggtaaa ttaatcaact
    25201 ctgccgagtt tgacggcttg gaatttgatg tggctttcac tgcgattgcc gataaattag
    25261 aggcaatggg tgtaggtaaa cgccaagtga atttccgtct gcgtgactgg ggtgtttctc
    25321 gtcaacgtta ttggggtgca ccaatcccaa tgatgaccac agaagagggc gaagtggtta
    25381 ccgttccatt acaagattta ccggtgattc tccctgaaga tgtggtaatg aacggcgtgc
    25441 aaagcccaat caaagccgac ccagagtggg cgaaaaccac ttacaacggc aaaccggcat
    25501 tgcgtgaaac cgataccttc gataccttta tggagtcctc ttggtattat gcacgttata
    25561 cttgcccaca atatcacgaa ggaatgttag attcagacga ggcaaactac tggctaccgg
    25621 tcgatcaata tatcggcggt attgaacacg ccacaatgca cttgctctac ttccgtttct
    25681 tccataaatt gttgcgtgat gcgggtattt taaattcgga cgaaccggcg actaaactgt
    25741 tatgccaagg tatggtgctt gccgatgcgt tctactacac cagcccaact aacgagcgta
    25801 tttgggtcag cccgacacaa gtcaccttag aacgtgatga gaaaggtcgt atcatcaaag
    25861 caaccgatcc acaagggcgt gagctggttc acagcggtat gactaaaatg tcgaaatcaa
    25921 agaacaacgg tatcgatccg caagaaatgg tggaaaaata tggtgctgac acagttcgct
    25981 tgtttatgat gtttgcctcc cctgcggaaa tgacattaga atggcaagaa tccggcgttg
    26041 aaggggcaaa acgtttctta ggtcgtgttt ggaatttagt ttatagctat gcccaaaatc
    26101 cggcaacgac tgcattagat gtgaatgcct tagacaagga tcaaaaagaa cttcgccgtg
    26161 atgtacacaa aacgattgct aaggtgagtg atgatattgg tcgtcgtcaa accttcaata
    26221 cagcgattgc tgcggtgatg gaattgatga acaagctcac caaagcccca cttgaaaccg
    26281 aacaagataa agccgtaatg gcagaagctt taagtgcggt ggtgcgtatg ctttacccaa
    26341 tcacgccaca tatctgcttt gagttatggc aagtgttagg taatgaacaa accatcgatt
    26401 ttgccccttg ggtacaagcc gatgaatccg caatggtgga agatgaaaaa ttagtggtag
    26461 tacaagtcaa cggaaaagtg cgtggcaaaa tcactgtttc agctactgct acggaagatg
    26521 aagttaaagc tatcgctaaa gccgatccaa acgttgctaa attcttagat ggtcagcaga
    26581 ttgtaaaaga aatctacgtt ccatttaaaa tgttgagctt tgcggttaag gcgtaacaaa
    26641 cacactctcc cttatatggg agagagaccg ataaatattt cgtgcagaaa tatttatcag
    26701 agagagggta caagcggtta ttttcttgga aaattttgca aaaaatagcc ccctctccct
    26761 gctttttctt ctaaacgaag aaaaacctgt ccctctccca caaggggaga ggggaataaa
    26821 caaggaaatc tcattatgat taaaaaactc tctctcatcg ctgtttttgc tcttaccgcc
    26881 tgcggttggc actttaaaaa caatgaggta ttacctgaat cgttcagaac attaaccttt
    26941 gaaagtgcag atcaacacag cgaaatgtct cgaattttac gcaatcagtt acaactaagc
    27001 gatgtaaaat tagtgcctag cacggctaat gtggcaaaat tacgtttggt ttcaacctca
    27061 acagacagca aagtggtttc tgtgtttaaa caagctcgtg aagcagaaaa aatcttaacg
    27121 ctaaacgttg atgcgattgc cgatattcca aataaagggc aatacccttt agaagtttcg
    27181 gttcatcgta ctttctttga tgactctcgt gcagctttgg caaagtctgc tgaaaaagag
    27241 atgattatga aagatatgta tgaacaagcc tctcgccaat taattatcaa aatggcgggc
    27301 ttaaaaaaag aactctcaaa ataatcattg taggggcgaa atatgtttcg cccattgttt
    27361 atctgtatgc aaaaaatctt tccggaaaca cttcaatttt cattaaacaa aggactacag
    27421 cccttttatc tattaacagg gaatgatctg ctattggtga atgaaaccaa agatgccatt
    27481 attcacaccg cccgtctcaa cggttttgat gaaaaaaaag aagttgaaat caaaaacgat
    27541 actaactggg aagaactttt cgaagcagct caatcaatgg gattgttttt tgcacgccaa
    27601 attatggtgc tgaattttcc ggattccccc acagcgacac agtttaaaaa actggctgag
    27661 ctttgtgcct ttattcatag tgatttgctc tttatcctgc acttacctaa attttccaaa
    27721 acaatggaaa aacaggcttg gttcacgcaa attgagctaa atctcgtgca aattaactgc
    27781 caaacaccgg aacttagcaa acttccacaa tgggtcagtc atcgagcaaa agcgatgaat
    27841 ttgcaaattg aaaatgaagc aaaccaactc ctttgttata gctacgaagg caatctactg
    27901 gcgcttaaac aggcattaca actgctacaa ctcaatgcaa ctgatggaaa aatcacgctg
    27961 acaaaagccc aagaagtggt tgaacactcc gcccaattta ctccatttca atggattgat
    28021 gcattatttg aaggaaaaat ccaaagagct caacgcattt tgtaccatct gcaaaatgaa
    28081 gaagtacaag cggtcgtttt attaagaatt gtgcaaaaag agttgatgat tttactcgaa
    28141 atcactcgca acccaaccgc agtcgtttcc agcgaacaat cactttttat cggtaattta
    28201 cgaacagaat ttgaccgctt gaaaatttgg caaaaccgca gaccttttta caaccagatc
    28261 actcaacgac tgacttacca aaaactttac gccctaatcc aacaactggc tgaattagag
    28321 cgtaaggtga aacaagaatt tagcgatgat atttggcaag aattagagcg tttttccgct
    28381 caatttacct aactaatact tttctaaatc ttcgctaaac aactccgctt cttcactggc
    28441 gatagagtgc ttttctgccg cccaatcgcc taaatcaata atttgacaac gctcgctaca
    28501 aaacgggcgg tatttacttt ctttactcca caccacttct ttttcacaag tcgggcagtt
    28561 tacaatagtt tctggcattc tttttccttt tttgctaatg ctaagtattt ataatgtaag
    28621 gcttgaacct gctctttaat taaaagcaag ccctgttcaa atggcaagtt attttcaatc
    28681 acatcatcag cataacttaa tctttcttca cggctgactt gggctgccat aatattttta
    28741 atggtttcaa ttttactgtt atcacgtttg gttgctcttt ccagttggat ttcaggagaa
    28801 acatcaacaa ccaatatacg atcacaatat tcggtgagct tattttcaat taaaagcggc
    28861 acgacccaaa tcacataaca agcggtcgat tcttgcaatt tttttaccat ttcttctcga
    28921 atggcggggt gaagtaaatt attgagccac gttttttcct cttcattgtt aaacacaatt
    28981 tggcgaagtt tagtgcgttc taatgctttt tcagcggtta agatctcttc accaaaatgc
    29041 tctacaatct tggctaaaag cggtgtaccc ggttcaacaa cttggcgagc caccacatca
    29101 gcatcaatta cctctacccc aagagctgaa aataaatcag acactgtggt tttgccactg
    29161 cctattccac cggttaagcc tacaacgtaa gtcataaaaa ttgattcctc aaaataaaaa
    29221 caaaccaatt ctagtaatta atccctctgc tttcaagcat tagcaccagc aacatcgctg
    29281 tgctaataaa cggaataaac gccaattttg ctaatttctg ctttttcatt agccaataag
    29341 ccaagtaata accaatccct agcaggctgg cggttaatat caataagagc attttttcga
    29401 gccgaaagag tggggataac gcaaccaata acaacatatc gcccgagcca aatgcctcct
    29461 ttttaaacat aagctgtgaa accaagccaa tcaacaggca aaaagcaagg gtaaaggtaa
    29521 aagtttggtg ttggaaatcg gctaaactct cccataacac caacaaaaat ataagcacga
    29581 ggtagcgaat atccgttagg taatagcaca tatccagcag tgataaaaaa cagagcagaa
    29641 taaaaatcgt gccgataata gggctttccg agcagagcat aatcaatgga aaaaggagaa
    29701 aaaatagatt tgccgatcgg ggttgtttcg gttgtagcac cgattgagcc aaaaactgtt
    29761 gctcggttaa gccaatattc actaatgaag ttgtttcttg gtaaatttgg cgattgactc
    29821 gaatggcaaa tcgagggatc tccgctttga gccaaaccgc aatgaagagg gaaaacagta
    29881 ttaacatcgc tactccacca aagcccccat atcaaaaatc ggtaaataca gcccaacaat
    29941 aatagtgccg acaatcacgc ccattatcaa cattagcatc ggttctaata gctgagacaa
    30001 aatatcaatt tggtaatcta atttttgttg gtacatctca ctaatatgct cacacatttt
    30061 cgccacattg ccacttttct ccccaacctc aagcatttgt gccatctcag caccaaacac
    30121 attcgggttg atcccttgtg aaaatttgta gccttgttgt aacaaattaa gcatagtgcc
    30181 aatttcttgc tgtaaaatcg ggtcatcatt tttctcggat aaaaacgacc tcaaggcaac
    30241 atcaagcgga atatgagcat tcagcattaa ggcaatattt tggctgaaaa agataatgcg
    30301 agattgctca ataattcggc taaacagtgg cattttagcc catagagcca ttttccactt
    30361 tgcgactaac ctgctctttt ttgcaaaaaa caggtaaaaa atgaccgcta gtagtaacgt
    30421 gccaagtaat actgtgcctt gctcaatcaa gcattcggaa agggtaaata aaatatcggt
    30481 aatgaggggt aaagttttgt cttttgagcc ataaagttgg gcaaactgcg gtacaatcaa
    30541 aaccaatagc ccgatagata aagcgagcga aatcagcaac acaatcaccg ggtaaaacag
    30601 gattttcttc acttttttcg ccaatttttc caatttcgct cggctttcag ctaaattagt
    30661 cagcataatg cccagttttc cgctttgttc gcccattttg attagctgaa tttcctgatt
    30721 ggtcacaaat tcacccaatt tctccaacga ttgtgaaaaa gcatagccac tttcaatatg
    30781 ctgaatcagc tcattaagcc atagataaag gcggatattt cggcagtttt gcaacaacat
    30841 agataatgcc tgttttaacg gcatggcaga attaaccaac aatgcaagct gtgatagcaa
    30901 gctattcacc tcttcttttt tcgggtttgc tgaaagcaca aaatttcggc taatccgaat
    30961 atgctgataa ccttgtgcca gcaaaaggtt ttccaacatg tctcgattcg ttgccaaccg
    31021 cttgccccgc tgtttttgcc caaatcggct ctgggcttgc caatgaaatt catagatttt
    31081 cgtcattgcc taagcaattt ctctttttgt ggttcaaaat gaatatctga ggcgttgtgt
    31141 tgcaaacaga acttaaataa attctctaaa atttggataa tcggatcgtt tctatccact
    31201 tgttccgaat gagccaatga atacgccgta ttctcctcat aaatctcact ctgccgacta
    31261 ctctgctcag gcgaaagggc attgagtaaa aacttcaatt catcggagga aatcaaaaca
    31321 ggctcaatct gtttgtggta gataaacgca aaaatctcac aggcattgag attttgcata
    31381 tcatcaatcc ctagccaaag ctgttcttct gtttcttgca gcggaatggc taaatatcgc
    31441 aataaaatct gcttttcatt gcaatttttc tgccactgct ctgctgaaat ctcgatgaac
    31501 ctttggctgt ttaaatccgt taccgaatac tccattttga tttcctcacc tcaaattagc
    31561 cgtttggtgt aacagttgtg ctacagaaat tagctggaaa taggctcgta tcattgccac
    31621 tacaacttgc cgtccaacta atcgtattat ttgagaacga cggtgttaag gtatagccat
    31681 aaccattcag agagcctttg ccggttaccg taatcacgcc ttttgttacc gcaattgagt
    31741 ttaggtattt agtggtatta ctactggttt tattcgcctg aatcccattc gtgccaccgg
    31801 agcagttatc caacccgcca ctgttgtaaa tacagacttc aacgtctgat ttatacgaag
    31861 cagatgcttg taataactcc gataacgccg ccttttgggt gtatgagtta taggaaggga
    31921 tcgcaatagt ggcaagaatg gcaataatcg caatcacgat catcaactca atgagggtaa
    31981 atgcccgatg cagttggcga atgccaagcg gtctaaaatg tacaattttt tgcatataaa
    32041 ttccttataa attgcaccaa caaattgtgg cgaatcaagt gtgcaaattt tttgcctgct
    32101 ttgcgaccgg caaattttaa tttttcgacg cagatcgaaa aaataaaatt tttatctgtt
    32161 tatccataca gttttttgtt agaatttgcc aaatttactc aattaggaat caagaatggc
    32221 aaaagcacct aaaaccgctt atgtttgtaa cgattgcggg gctgaatatt cccgttggat
    32281 ggggcaatgt aaggaatgta aggcttggaa taccatcagt gaagtgcggt tgatttccag
    32341 caaagaagcg gcgaaatctg accgctttag cggctatgcc ggtgaaacgt caggcaaagt
    32401 acaaacgctg tctgacatca gcttgcagga agtgcctcgc tttagcagtg gctttaacga
    32461 gctggatcgt gtactcggtg gcggcgtagt gccggggtca gcaattctga tcggcggtca
    32521 cccgggggcg gggaaaagta cgttattact gcaagtaatg tgcggtttag cccaacatct
    32581 gccaacgctg tatgtcaccg gtgaagagtc gttacaacaa gttgcaatgc gggcaaatca
    32641 cttaggctta ccaacggcaa atctgaaaat gttgtctgaa acctctgtcg agcatatttg
    32701 caatattgca gatcaagaaa agcccaaaat tatggtgatt gactcgattc aggtgatgca
    32761 cttgtcggat attcagtcct cacccggcag tgtttcacaa gtgcgtgaat gtgcatcttt
    32821 ccttacccgc tacgccaaaa cccgccaagt ggcgattatt atggttgggc acgtcactaa
    32881 agatggcacg cttgccggcc cgaaagtgtt ggaacacgcc atcgatgctt ccttgctgtt
    32941 agaaggcgaa tccgattccc gtttccgtac tttgcgtagc cagaaaaatc gttttggtgc
    33001 cgtcaatgaa ttgggcgtat ttgcgatgac cgaacaaggg ctaaaagagg ttaaaaaccc
    33061 atctgcgatt tttttaagcc gtagcgaaga gcaaacgccg ggcagttcgg tgatggtgtt
    33121 atgggaagga acacgcccat tgttggttga aattcaagcc ttagtcgatc actcaatgct
    33181 ggcaaaccct cgccgtgtgg cggttggctt agatcataac cgtttatcgc tattacttgc
    33241 ggtgctacac cgacacggtg gcttgcagat gtcggatcaa gatgtgtttg taaatgtggt
    33301 cggtggggtg aaagtcacgg aaaccagtgc cgatttagcc ttaattttgg cgttaatttc
    33361 cagcttccgt aaccgtccat tgccgcaaga tttggtgatt tttggggaag tcggcttagc
    33421 aggggaaatc cgcccggtta ccagtgggca agagcggatc agcgaagccg caaaacacgg
    33481 ctttaaacga gcgataattc ctcacggcaa tgtcccgaaa aaggcaatta aagatatgca
    33541 ggtgttcacc gtgaaaaaac tcagtgatgc gttagatatt ctgtataatc tctaaaatca
    33601 agaaccgctt gtaagtctgt cttacaagcg gtctgtttta ggtaaaaatt tacgaatgtg
    33661 cggcagttgg tctattgcct ttggaaatat agtataaatt aatacacacc accaacgcat
    33721 ttaagccggt gaccggaatc gagccaatca gcaaaccata aacaacgaaa caggcacaac
    33781 ccagcgaatt caccacccgc agtttaatca catcttttaa taaaaaagaa caggcaacta
    33841 aaatcatcgc cacatagccg agtatttcaa catagtccat ttagaacctc ctcaaataaa
    33901 ataaactgcc gatattttaa aggctgtttt tttatttgta tctagctcta aaaatagcaa
    33961 aggtttgcgt tcacattttt acttttaacc gcttagttat taaacagatt ttcgttctaa
    34021 acacataaaa cggcaacgga aagggttatt ttcatcaatt tctgaccact tttcttctac
    34081 taacgcccaa ttttgttcat caaattcaaa gaacgtatcc ccttcaatgt ctgcttgaat
    34141 ctcagttaaa taaagtcttt ctgctagtgg taaggcttgc ttaaacagtt cccccccacc
    34201 aataatcatt atttcagctg tatttgcaaa atttttcgct aattcaaccg cttgttcgag
    34261 cgattgtacc gcataagccc cttccaccgt aaaacctgaa cgggaaagaa taatattagg
    34321 gcgttttggc aataacctgc cgatagattc ataggttttc cgccccataa tcaccggttt
    34381 gccgagcgtg ttttggcgaa accaggctaa atctaccggc aaatgccacg gcatttggtt
    34441 atctttgcca atcaccttgt tgattgttcg ggcaacaata atgctgattt tcattttttc
    34501 tcctgttatg caaattggtt tgattttata tcaaattaag cgtgatttta caccctaaaa
    34561 gtgatacttt tggcaacatt atttttagtt acaagtgaac agattatgtc aaaaactatc
    34621 gtgataaaat ttggcaccag cactctcaca cacggcacta aaagtttaag ccgtccttat
    34681 atgttagagc tggtaaagct caattacacc aacagcatcg tgtgattgtg gtgacatccg
    34741 gagctgccgc tgccggtcgg gactatttag gacaccctga actccctaaa accttagcct
    34801 ctaagcaaat gttagcagcg gtcgggcaaa gtcagctcat tcgagtgtgg gaaaatttat
    34861 tcgatattta taatatccat atcgggcaga tgctcttaac ccgagccgat ctggacgatc
    34921 gtgaacgttt cctcaatgcc cgtgataccc ttgatgccct gcttgcacaa aaaatcattc
    34981 cggtgattaa tgaaaacgat gccgttgcta ccgaagaatt taaagtaggc gataacgata
    35041 atttatcggc tttagttgcg attttggctc aagcagatca gctctattta ttaaccgacc
    35101 aagaaggctt attcagtgcc gatcctcgct caaaccctga tgctaaattg ctctcggtgg
    35161 tggaaaaaat cacgcctgaa atccgccaaa ttgccggtgg tagttcaacc ggtttaggca
    35221 cgggcggaat gagtaccaaa attactgctg ctgatattgc tacccgctcc ggtgtggaaa
    35281 ccatcatcgc ctccggttct cgcccaaatg tgttagttga tttagcaaac gggcaaaaca
    35341 tcggcagtaa atttttagtc caatctgacc gcttggaagg cagaaaacgc tggttatttg
    35401 ctgctccgca agtgggtgaa attgtggtag atttcggggc ggaacaagcc ttgcttcaac
    35461 ataaatcatt actgccggtg ggagtttctg ccatcaaagg cgaatttact cgaggtgaag
    35521 taatcaaaat tttcagccaa gacggcaaag ccttagcact cggcattgcc cgttatagca
    35581 gtgaggcatt gctgcgaatt aaaggtaaga aatcaaaaga aattgaatcg ctccttggtt
    35641 atgaatttgg ttctgttgca gtgcatagtg acgaaatggt ggtgtattaa tggaattggg
    35701 aattgatgtt ttagcaatct tgtttgccgt tgcgaccctt gccggcttta tcgatgccat
    35761 cgctggcggc ggtggcttac tcactattcc tgcattaatg gcagcagggc ttcctccggc
    35821 aatggcactt ggcacgaata aattacaggc gtgtggcgga tcactctctt cctcactcta
    35881 ttttattcgc aaaaaagctg ttaatttaaa acaaatttgg ttgctaattc tatgcacttt
    35941 tttaggctct gccgccggta ctatttttgt gcaaatgatc aatgtcgatt tcttaaaaac
    36001 attgttgcct ttcttaattc tgattatcgg catctatttt ttggtcagcc ctaaaattgg
    36061 cgatgaagat cgtaaacaac gcattagctt taccctattt gcctttaccg ccgctgtcgc
    36121 tctcggtttt tatgatggaa tgttcggacc ggcaaccggc tcttttatga ctctggcatt
    36181 tattttgctg ctcggcttca acctctctaa agccgtagca cacgccaaag tgattaattt
    36241 tacctccaac tttgcctcat taattttctt tacgcttggt ggtgcggtaa tgtggaaaat
    36301 cggcttaatt atgatggtcg ggcaatttat tggcggcaca ctaggtgcga aaatggtgat
    36361 gacaaaaggc aaaaaattaa tccgtccgat gctagtgact atgtctttta tgatggtcgg
    36421 caaaatgctc tacgagcaag gaatttttaa ttaacaatga ataaagcaaa acgtattgag
    36481 attttaaccc gtttacgcaa tgaaaatcca aaaccgacaa ctgaactcaa ttacagtaat
    36541 ccgtttgaat tattaattgc cgtgattcta tccgcccaag cgaccgataa aggcgtaaat
    36601 aaagccaccg ataagctctt ccctgtcgcc aacacgccac aagcgatttt agatttaggc
    36661 gtagatgggc taaaagagta catcaaaact atcggcttat ttaacagcaa ggcagaaaat
    36721 attatcaaga cctgccgaga tctgatcgaa aaacataacg gcgaagtgcc tgaagacaga
    36781 gatgccctcg aagcccttgc cggtgttggc agaaaaaccg ccaatgtggt gatgaatacc
    36841 gcattcggac accctacgat tgcagtcgat acccatattt tcagggtgtc gaaccgcacg
    36901 aacttcgctc ccggcaaaaa tgtagttcag gtagaagaaa aactgctcaa agttgtgccg
    36961 gacgaattta aagtcgatgt tcaccattgg ttgattttac acggacgcta cacttgcatt
    37021 gcccgtaagc ctcgttgtgg ttcttgtatt attgaagatt tatgtgagtt taaagagaag
    37081 acggaataac attatctggt ggtatttaat gaataactcc cctctttaga aaagtagggg
    37141 ctgggggaga tttgatacta tttcaacatt tcgtatgagc gtatagaaat tctgccaaat
    37201 cttccctatc ccctctttgc taaagagggg aactagatcg agcgtaaaat ataacaagcg
    37261 gtcatttttt tgagattttt tgcaaatgga tatttattta gtcggtggag cagtgcggga
    37321 tcaacttctt gggctgccag tgaaagatag agatttttta gtggttggcg caactgccga
    37381 acaactttta gcccaaggat ttcaacaagt tggggcagat tttccggtgt ttttacaccc
    37441 tgaaaccaaa gaagaatatg cccttgctcg ccaagaacgc aagaacggca atggttacaa
    37501 cggctttatt tgcgatttct ccccaaacgt cacgcttgag caagatttga tccgccgaga
    37561 tttaaccatt aacgccatcg cccaagatga aaacagcaca ctttttgatc cttatggcgg
    37621 tgtgcaagat ttagaaaatc ggctactccg ccacgtttct cctgcattca gcgaagatcc
    37681 gctacgggtg ctacgggttg cccgctttgc cgcccgtttt cattcatttg gttttaccat
    37741 tgctcctgaa accctgaaat taatgcgaga gatggtacaa agcggtgaat tgaaacattt
    37801 aaccgctgaa cgtgtgtggt tggaaacaca gaaagccttt gaaaccgata atccgcacgt
    37861 ttattttgac attttacgct taattggggc attaaaagtg ctattccctg aactggatcg
    37921 tctgtttggc gtaccacaac cacctcaaca ccaccctgaa attgactccg gcaaacacac
    37981 tctaatggtg ctacaacagg caaaaagact tgcaaaaaaa gcggaaaatc cgaccgcttt
    38041 actctttgca gccctttgcc acgacttagg caaaggctta acgcctgctg atattttgcc
    38101 tcatcactac ggacacgaag tcaaaggcat tcaaccaacc cgagagttag ccaatcggtt
    38161 aaaaatccca actgagatta aagattttgc ccttttagtg accgaatacc acacgcattg
    38221 ccataaaatg gcggaacttc gccctgaaac ggtaataaaa ctgtttaaca gcctagatgt
    38281 gtggcgtaaa ccaaaccgct tttttgattt cctgttcgcc tgtgaagcgg atgctagagg
    38341 gcgattaggt tttgaacagc ggaattaccc ccaacctgaa ttggcgaaaa aatattacca
    38401 agccgcaatc gaaattgatg tgcagcaagt tattcaagac ggttttgaaa aacaagcaat
    38461 tcgggaagag ctgaataaac gcagagtgta tgcggtaaga agcatcaagc tagatagcga
    38521 cctaataaac cattaggtta tccgccactt tctcaaaatc aattcccaaa gttcaagttg
    38581 gttaaatgta tggattcttt aaaaggtggc gttattttag tgaatttata atgttcctat
    38641 gctcttgatt gttgtagcca caatttcagg gtaaagatca gcaataaccc aatgccgaaa
    38701 ttggcaatag tgaagccggc taacccaata aaagataaag gttcaaattg ataaaattca
    38761 atcgcacctc ttgccattgc agctaaacca aatgaaaatg cccagaaacc catcgagaag
    38821 ccattttcag ctatccattt gaggtttcgt aaaataaaac agaattgtaa gaatccatat
    38881 ccccataaca tttttgccaa caagtctaat tctccaccca tagaaaaata ggcagaagcg
    38941 cccacaaatg ccggagcaag aacaattcct aaagatggtc tagcaatcgg aaaaagcgga
    39001 ctaagccgaa ttctttgtaa catagtgggt tcatatacta accaggaaat tatgccaatg
    39061 cctaaaaata aagaggcaat ttcactgtag ccaattaagc ttaatgaact tgccatagtg
    39121 aagctccctg caattggcgg taggtaatgt accgggtgta gcgtttccgg caaaaattgt
    39181 gtgcctttcc ataatccacc aataataaaa accaagaaaa ttaaatgcac aatgacgcct
    39241 aaaaaaatca atagttcgcc taaataaaaa tcccatacat agagcacatc accgcttatc
    39301 atggttgtca ttgtcactaa ggaaaataaa caacattgca ctgggtgcat aaattctgct
    39361 ttaatctgct caaaacaaaa aaacacacga tacacataac agcctaataa ggctacccaa
    39421 agtatgactg acgttccagc aatgatatta ccaattgttg tagcataagg agtgataggc
    39481 gttgcattta accaggcaag gcctaacgcc cccagcccta atggcattgc aaaaaagcct
    39541 gtagttattg gaaaaggttt agattcactc ataaatttat cctcatattg aaataatgca
    39601 taactgtatt cggctttata acagtcatat gtaaaaaatt ttatttttct aaccgcttgt
    39661 aataagaaaa acctaaagca cttgctttag gttcctacta ctattcatac tcttctagcc
    39721 agatagttaa aatcgcttca agaatatgtt catttgatgc ttccggatca tcatcgaaat
    39781 cttccatttc acaaatccat ttatgcatat ctgtaaaacg aaccgtggtc gggtctaaat
    39841 ccgggttcat atcatacaga ttttcagcga tcattcgggt atctgtccat ttcattagtg
    39901 cgctgcctca tttgcgtgat ttaaattata ttttggaatt tcaacaacca aatcttcttc
    39961 accaacaata cattggcaac ttaaacggct gtctatttcc aatccccaag ctttatccag
    40021 catatcttct tcttgatctg aagtatcatt taacgaatca aaaccctcac gaattaccac
    40081 gtggcaagtg gtacaagcac aagaacagtc gcaagcgtga tgaatttcaa cgcctgcttc
    40141 gtgtgctact tctaataaat tatctccggc tttagcttca acaaccatac cttccgggca
    40201 aaattcttcg tgtggaagaa aaacaatttt tggcatttta aagtttcctt attactaatc
    40261 ccattcaata ttatccagat gttcgccgat gaagtttaaa atctcttcct cattttcaac
    40321 cagataaaca ataatcttct ttaattccct tgctttctct ttacttttct cggaaagcgg
    40381 ataggtaaca taataacatc ctacgtccgg atcttcttct aataactcta ataggcttgg
    40441 acgataaata ctaagataag cccgtaaaaa tgcagcccag ttataaccgt tcatataggc
    40501 aacatcatat tcagtattaa tccgctcgcc caactcaaaa atgcggttat cctctaaatc
    40561 gaattcaacc gcaacggttc tgaattgttc tgaaatctcg gtttttacat agttagacat
    40621 actgcctcct tatgctaaat cgtccatttt tttaccggct aatgcttttt gaattgataa
    40681 attcatacgc cgtgctgcaa attcttgcgt agctaaatcc acctctttga taccttgctg
    40741 aattgcaaag cgatcagtac cttcttttaa ttcgattaaa cgggctaatt gtgcttcaat
    40801 cgcttggaac tcctccacac tcaacactgc agagccatct tctttcaatg ccgttaccac
    40861 gctgtcaatc actcggtcgg cttctactcg ctgttcaact aactggcggg cttccatatc
    40921 ttctttggca tttgccattg aggatttaat catctgcgta acttcttcat cggttaagcc
    40981 ataagacggt ttgatttgaa ttgaagcctg cactttggtc gatttttcca ttgcagtcac
    41041 gtttaataaa ccgtctgcat caacttggta agttacccga acctgtgcaa cccctgctgc
    41101 cattgccgga atgccacgca aagtgatttt accgagagag cgacaatctt cgactaattc
    41161 acgctcacct tgtaaaacgt ggattgtcat cgccgtttga ccgtctttac cggtagtaaa
    41221 ctcttgagct cgagctactg ggatagtagt gttgcgtgga ataattttct ccaccaaacc
    41281 acccatcgtt tcaataccaa gcgaaagtgg cacaacatca agcaacaaca tatcggaatc
    41341 cggtttgttt ccaactaaaa tatccgcctg cactgccgca ccaatggcaa caactttatc
    41401 cgggtcgata gaggttaatg gcgttttacc gaaaaactcg ccgacttgtt cacgcacaaa
    41461 cggtacacgg gtcgaaccgc ctaccattac cacttcacga acctcttctg cttcaactcc
    41521 ggcatctttc aaggctcggc gacaagtcat cagcgagcgt tttacaagcg gttgaattag
    41581 ctcattaaat tgcgaacggc ttactacgcc tgtccaattt gcaaatttca cttcaaattc
    41641 aaccgcttgc gaaagtgcga ctttggtttg agttgccaaa gttaataatt cacgctgctc
    41701 gcttgccgtt tgaggtttgt aatttgcctg ttcagcaatc cattccgcta ataggtggtc
    41761 gaaatcatcg ccaccgagtg cggtatcacc tgcggttgct aatacttcaa acacaccttt
    41821 gcgtaaacgc aaaatagata aatcgaaagt accgccacct aaatcgtaaa cggcaatcac
    41881 accttcttgt ccgctttcta aaccataagc aatcgctgct gccgttggtt cattgagcaa
    41941 acgtaataca tttaaacccg caagacgtgc ggcatctttg gtactttggc gttgtgcgtc
    42001 atcaaaataa gccggtacgg taattaccac gcctgaaagc tcaccggcta aacgcttttc
    42061 ggcaaattgt gccaagtggc ttaaaatatc cgccgaaact tcaatcgggc ttttattacc
    42121 ttgcgctgtt tggattaaag gtaaaccgtt ttcgctcgcg acaaattgat aaggtaaatt
    42181 ttgataacgt tgctgaacat ctgccagaga acgcccaatg agacgtttag ctgaaatgac
    42241 ggtatttttc gggtcaagtg cagctttggc aaaggcttca atcccaactg ttttttctgt
    42301 ttccgcataa tgcactactg acggcactaa tgctctttct tgttcatcaa gcaatacctg
    42361 agtctgcccg ctacgcacag ttgccactaa tgaattggtt gtacctagat caattcctac
    42421 cgctaattta tgttgatgtg gtgcagcggt ttgaccgggt tcagaaattt gtaataatgc
    42481 catttattta tctctttatc tttctaactt agttctctat tgtattaaat ggagtgaaat
    42541 atattgttta agagaagctg gagattcccc tctccctgat ttttcttctg aacgaagaaa
    42601 aatctatccc tctcccacaa ggggagaggg ttattctttc aaaaattcaa aacaactaaa
    42661 aaattagcat tctccttttg taggagaaaa tgttgttctt tcaaaattct aaaataaccg
    42721 caaaattaac tctcgcccct tgtgggagag agacagataa atcattcgtt cagaatgatt
    42781 tatcagagag agggggaatt accctctcgt aatttcataa aaaattgtat ctaaaacagc
    42841 ctctagatcg ctatgaattt gattattcca aaatcttaaa accctgaacc cctgaccgtt
    42901 taaaaaatga gttcgtagat tatcatagtt ttgttgatct aaatgttgtg aaccatctaa
    42961 ttcaataact aatttcgctg aaaagcaaac aaaatcgaca atatagttac ctataacttg
    43021 ttgccgttta aattttatcg ctaaaaatcg ttttgctctt aatgcttgcc aaagaacctt
    43081 ctctgcttct gtcatattag tccgaagctg tttagctctt tggcataaaa ttgctttcat
    43141 tt
//
LOCUS       DANLZC010000005        37662 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.005, whole genome shotgun sequence.
ACCESSION   DANLZC010000005 DANLZC010000000
VERSION     DANLZC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 37662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37662
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.005"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            314..1672
                     /locus_tag="PXE44_000228"
     CDS             314..1672
                     /locus_tag="PXE44_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HDL5587776.1"
                     /translation="MITIKKGLDLPIAGKPEQVIHNGNTVTEVAMLGEEYVGMRPSMK
                     VREGDVVKKGQVLFEDKKNPGVVFTAPASGTVTAINRGEKRVLQSVVIKVEGDEQITF
                     ARYEASQLASLTSEQVKQNLVESGLWTAFRTRPFSKVPALDAEPSSIFVNAMDTNPLA
                     ANPEVIIAEHQQAFFDGLTVLSRLFESKKVIHLCGSADSNILNAAQSRQINNVETNRF
                     KGPHPAGLSGTHIHFIDPVGATKQVWYLNYQDVIAIGKLFTTGELFTDRVVALAGPQV
                     KNPRLVRTRLGANLSQLTANELNAGENRVISGSVLSGAIATGVHDYLGRYALQVSALL
                     EGREKAFFGMIAPYSDKYSITRTTLGHFAKKLFNFTTAVHGSERAMVPTGAYERVMPL
                     DIIPTLLLRDLAAGDTDSAQALGCLELDEEDLALCTFVCSGKNNWGPLLRQALDKIEK
                     DG"
     gene            1675..2910
                     /locus_tag="PXE44_000229"
     CDS             1675..2910
                     /locus_tag="PXE44_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HDL5587777.1"
                     /translation="MGLKNLFEKMEPAFHKGGKYEKWYTLFEAAYTIFYTPGTVTRKD
                     AHVRDAIDSKRMMLIVWLALFPAMFYGMYNVGAQGILAALKMGTLTDLVANNWHYSLA
                     DSLGSVADAGWGTKMLLGATYFLPIYLTVFAVGGFWEVVFAMVRKHEINEGFFVTSIL
                     LALIVPPTLPLWQAALATTFGVVVAKEVFGGVGKNFMNPALAGRAFLFFAYPGQISGD
                     AVWVAADGFSGATALSQWAVGGQGALQHVATGETITWMDAFLGNIPGSIGETSTLMLI
                     IGAAIIVFARIASWRIIAGVMIGMAATATLFNVIGSDTNPLFSMPWHWHLVLGGFAIG
                     MFFMATDPVSAAFTNKGKWAYGILIGFMCVLIRVVNPAYPEGMMLAILFANLFAPIFD
                     YLVVQGNIKRRLARVANNG"
     gene            2903..3676
                     /locus_tag="PXE44_000230"
     CDS             2903..3676
                     /locus_tag="PXE44_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDL5587778.1"
                     /translation="MAKFNKDSVGGTLTVVVLLSLICSLIVAGAAVMLKPTQDIQKQL
                     DKQKNILQAAGLLQKDTNVQETYTKFIEPKIVDLATGDYVEGVTGFDARAAAKDPAQN
                     VVIAPEDDKANIKTRAKYAEVYLVKDESGKTTQVVLPMHGNGLWSIMYGFVAVQPDAN
                     TVNGITYYEQGETAGLGGEIANPNWQKNFVGKKLYNEQNQVALRVGKGASADKDHGVD
                     GLSGATLTSNGVDGSFKYWFGQNGFGPYLAKFKAAAGAN"
     gene            3676..4302
                     /locus_tag="PXE44_000231"
     CDS             3676..4302
                     /locus_tag="PXE44_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005717817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HDL5587779.1"
                     /translation="MADNNNLKKLLLSPIVDNNPIALQILGICSALAVTTQLQTAVVM
                     AIAVSLVTAFSSMFISMIRNYIPNSIRIIVQMAIIASLVILVDQILRAYAYDLSKQLS
                     VFVGLIITNCIVMGRAEAFAMKSGPVESFVDGIGNGLGYGAMLIIVAFLRELIGSGKI
                     FGITILETVQNGGWYQANGLFLLAPSAFFIIGFVIWAIRTWKPEQVEK"
     gene            4304..4900
                     /gene="nqrE"
                     /locus_tag="PXE44_000232"
     CDS             4304..4900
                     /gene="nqrE"
                     /locus_tag="PXE44_000232"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005529392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HDL5587780.1"
                     /translation="MEHLLSIFVKSIFIENMALSFFLGMCTFLAVSKKVSTAFGLGIA
                     VIVVLGIAVPANQLVYTHVLKEGALVEGVDLSFLNFITFIGVIAALVQILEMILDKFF
                     PALYSALGIFLPLITVNCAIFGGVSFMVQREYNFVESVVYGVGAGTGWMLAIVALAGL
                     TEKMKYSDVPAGLRGLGITFITVGLMALGFMSFSGIQL"
     gene            4911..6140
                     /gene="nqrF"
                     /locus_tag="PXE44_000233"
     CDS             4911..6140
                     /gene="nqrF"
                     /locus_tag="PXE44_000233"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HDL5587781.1"
                     /translation="MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDP
                     EKGITLPAGGKLLGALASKGIFVSSACGGGGSCGQCKVQVKSGGGEILPTELSHISKK
                     EAKEGWRLACQVNVKSSMDVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEE
                     VPFRAGGYIQIEAEPHTVHYKDFDIPKEYHEDWDKFDLWRYTSKVDEHIIRAYSMASY
                     PEEKGIIMLNVRIATPPPRNPDVPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAE
                     MVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSFWYGARSEREMFYVEDFDGLQAENDNF
                     KWYVALSDPLPGDREDYFRGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIGML
                     KSLGVEDENILLDDFGG"
     gene            6252..6686
                     /locus_tag="PXE44_000234"
     CDS             6252..6686
                     /locus_tag="PXE44_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL5587782.1"
                     /translation="MEQLDITVLENAFNSLEKTIVSLENQAWFNRQEAIIQDTLIAGA
                     IQKFEFVYELSIKMLKRQLKNIESNDTDIDSTEFRDILRMAAQYGLIESPEVWLDYRK
                     MRNITSHTYDQNKAQQVYNGISNFLESTYFLVKQLQKRNSYD"
     gene            6679..6972
                     /locus_tag="PXE44_000235"
     CDS             6679..6972
                     /locus_tag="PXE44_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL5587783.1"
                     /translation="MIDLTESQRQQVLDIFKKYLPDTPVWVFGSRIKGTAKAYSDLDL
                     ALITKQPLTIRQQTELELAFTDSDLPFTVDLIDWASCNESFKQIILQRYEVLI"
     gene            7021..8055
                     /locus_tag="PXE44_000236"
     CDS             7021..8055
                     /locus_tag="PXE44_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL5587784.1"
                     /translation="MKKFFLLLIAFFTLSACEKAPEQITLSGKTMGTTYTVKYIDNGE
                     VENLISPEKMKNELDGLLIEVNNQMSTYQQDSEISRFNQAKQANQAVEISQDFAKVVQ
                     EAIRLNKVTEGALDVTVGPLVNLWGFGPDKRLNKVPSDEQIAERSAYVGIDKILLEMS
                     SKPKLTKSAANLYLDLSSIAKGFGVDKLAEHLETIGVSNYLVEIGGELRGKGKNLQGL
                     DWRIAIEKPVMTQGQAAQITVPLHNLAMATSGNYRNYFEDEKGNRLSHIIDPKQLRPV
                     NHNLASITVFAPTTMTADGLSTGLFVLGAEKALAVAEREKLAIFLIVKNGDKFETQMS
                     SEFSKLIQSQ"
     gene            8068..8313
                     /gene="nqrM"
                     /locus_tag="PXE44_000237"
     CDS             8068..8313
                     /gene="nqrM"
                     /locus_tag="PXE44_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HDL5587785.1"
                     /translation="METVLLTFGFFVAVIFAMSIGFIVKGKTIKGSCGGITALGMKKM
                     CDCEEPCDNLKSKIADGTADPEEVARFNKEPQFYEVK"
     gene            8315..8731
                     /locus_tag="PXE44_000238"
     CDS             8315..8731
                     /locus_tag="PXE44_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL5587786.1"
                     /translation="MNRDIRWKQRFDNYKKSVSYLQAEAEKYADTDIDVIKKGIIQSF
                     EITHELAWKLMQDILKWEGEVDIYGSKTASRLAFNRGLITQGEVWLEMIKSRNITVHT
                     DDETILTEEFNKIIKAYLPLFIEFEQRVETLCQTLD"
     gene            8713..9024
                     /locus_tag="PXE44_000239"
     CDS             8713..9024
                     /locus_tag="PXE44_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL5587787.1"
                     /translation="MPNFGLSERATKTITDILAKYPEVEQAIIYGSRAKGNYREGSDV
                     DLTFKGKDLTYDIVKRIWGDLDDSDSPYLFDLSIYHQLTNANFIEHIDRVGKVFYQRE
                     L"
     gene            9135..10298
                     /gene="mnmA"
                     /locus_tag="PXE44_000240"
     CDS             9135..10298
                     /gene="mnmA"
                     /locus_tag="PXE44_000240"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL5587788.1"
                     /translation="MTTQLTSKTYDTHFPKLTPEQLAENSKKKVIIGMSGGVDSSVSA
                     FILQQQGYQVEGLFMKNWEEDDDTDYCTAAAELADAQAVADKLGMKLHKINFAAEYWD
                     NVFEYFLAEYKAGRTPNPDILCNKEIKFKAFLEYAAEDLGADYIATGHYVRRTSDDQD
                     AKLLRGLDENKDQSYFLYALNKKQIGQSLFPVGEIEKPIVRAIAEDLGLATAKKKDST
                     GICFIGERKFKDFLARFLPAQPGEIRTVEGKLVGRHDGLMYHTLGQRKGLGIGGVKGL
                     SEDPFYVVEKDLINNVLIVAQGHDNSALLSSGLIAKQLTWVDMQPLRENLRCTVKTRY
                     RQADIACEIQVIDDETIRVMFDEPQIAVTPGQSAVFYLGEVCLGGGIIESQLK"
     gene            10415..11794
                     /gene="argH"
                     /locus_tag="PXE44_000241"
     CDS             10415..11794
                     /gene="argH"
                     /locus_tag="PXE44_000241"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDL5587789.1"
                     /translation="MALWGGRFTQQADAKFKYFNDSLRFDYRLAIQDIEGSIGWAKAI
                     TSVGILTVAELEQLIAALKEVRAEVESNLTIILKDDAEDIHSWVESKLIEKVGDLGKK
                     LHTGRSRNDQVAVDIKMWCKVQAVALQERIRALQERLVSVAEENQESVMPGYTHLQRA
                     QPVTFAHWCMAYYEMLERDFSRLSDANKRMSTCPLGSGALAGTAYGIDRDLLARDLGF
                     EEATRNSLDSVSDRDHILELLSTASISMMHLSRFAEDLIIFNSGESGFLEMSDRVTSG
                     SSLMPQKKNPDACELIRGKSGRVFGALNGLLTTLKGLPLAYNKDMQEDKEGIFDALET
                     WQACLEIAELVLVDIQVNTERTREAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VYAISKKEPLEALSINEFQQFHHSIGDDVYPILSLESCLEKRCAKGGVNPQRVAEAIA
                     VAKANLASK"
     gene            11962..12960
                     /locus_tag="PXE44_000242"
     CDS             11962..12960
                     /locus_tag="PXE44_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HDL5587790.1"
                     /translation="MTESVNSTQAGTALSLGQQLKAAREALNLSITDVAQKTNLKKSH
                     IESLENDIFILQNVAPTFVRGYVRNYVKFLRLPESLISSVNYGEVTIPKEVTKVSSVK
                     PSSNSQGKTLKYLTVLVLLAALGMTLLWWWQGYQKEQENREQFVSSAPVAEVANTATN
                     NDASAQTSVPVQQPIQVSEQPAQVAETVVAAEPALTEKVEPTQAASSPVTQEDVKVVE
                     EVKQADATTAQVEAVNVLQQSQSEQAPTEQPVAVTNDELRIEITGSQSWITVRGAKNK
                     RLAEKLYNNGETLSFNENEQYRLTIGAPANVKVYYKGQEVPLKIDGRVARIHLPLQ"
     gene            13061..14173
                     /gene="ispG"
                     /locus_tag="PXE44_000243"
                     /gene_synonym="gcpE"
     CDS             13061..14173
                     /gene="ispG"
                     /locus_tag="PXE44_000243"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDL5587791.1"
                     /translation="MIHQSPIKRRVSKKIYVGNVPVGGDAPITVQSMTNTRTTDVEAT
                     VAQIKSLERVGADIVRVSVPTMDAAEAFKAIKQQVSVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRTVVDCARDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLES
                     ALRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARAGSV
                     KSAVGLGLLLAEGIGDTLRVSLAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EIDVIATVNELEQRLEDIITPMDVSIIGCVVNGPGEALVSDLGVTGSNKMSGFYLDGV
                     RQKERFDNAKLIDQLEAKIRARVAQQHNRIDIEQIG"
     gene            14179..15450
                     /gene="hisS"
                     /locus_tag="PXE44_000244"
     CDS             14179..15450
                     /gene="hisS"
                     /locus_tag="PXE44_000244"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL5587792.1"
                     /translation="MAKTIQAIRGMNDCSPSESPLWQWVEQKVRNVLASYGYSEVRMP
                     IVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRPEGTAGCVRAAIERGWIYNNEQ
                     RLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIANPEIDAELIILTARLWKELGIDQHV
                     SLQLNSIGSLEARANYRSALVKFLENHTALMNEEEKERLVKNPLRILDTKNEALQNVL
                     NDAPKLLDYLDDDSREHFTQLCALLDAMGIRYEVNPKLVRGLDYYNKTVFEWVTSALG
                     SQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLERLVLLVQEVNKEIDLPKTVDVYIVF
                     SGEGATLNAFQLAEQIRSELPHVGVMTHCSGGKFQKQFKRADKVEAQIALVIGESEVA
                     EKQVVIKDLRSGAEQITIAQSELIAELSKRF"
     gene            15460..16080
                     /locus_tag="PXE44_000245"
     CDS             15460..16080
                     /locus_tag="PXE44_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL5587793.1"
                     /translation="MSNEYLNKTEEQQFEDAKNWFKENGTPILVAIILACAATAGWNF
                     WKNHQLETAQKTSASYQSVMESYLQNPSKNQPLVEKFIADNKDSSYAAFAQFEVAKQA
                     VEKGNFSAAKTALQSVLTYSDSTLQSIARFRLAQIDFQLKSYDEALASLAQIQDKAWE
                     LRKQILTGDILAAKGDIPAAKSAYEQAKTTANPQEQTLIDVRLNNL"
     gene            complement(16143..17246)
                     /gene="dnaN"
                     /locus_tag="PXE44_000246"
     CDS             complement(16143..17246)
                     /gene="dnaN"
                     /locus_tag="PXE44_000246"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL5587794.1"
                     /translation="MQITITREQLLKPLQQVCGVLSSRPTLPVINNLLLEIEGNRLSL
                     TGTDLEVELTTQAQLLQAVDFAGKFTIPAKKFLDISKSLPDDSVISVQFEEDKAIIKA
                     GRSKFSLATLPASDYPNLMDWKPEVDFSIDQSTLARLIDATQFSMANQDARYFLNGMK
                     FETEGNLLRTVATDGHRLAVCTMVLNQELLTHSVIVPRKAVLELSRLVASNDETVRLE
                     IGSSNIRITMNGIVFTSKLIDGRFPDYRRVLPRNADRILEAETETLKRALMRAAILSN
                     EKFRGVRLTLSNNLLKITANNPEQEEAEEIIDVAYDSPEMEVGFNVSYVLDVLNTLKC
                     QRVRFSLVDSSSSCLIEDCDNSSAEYVIMPMRL"
     gene            complement(17265..18614)
                     /gene="dnaA"
                     /locus_tag="PXE44_000247"
     CDS             complement(17265..18614)
                     /gene="dnaA"
                     /locus_tag="PXE44_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL5587795.1"
                     /translation="MEQIVSSLWSDCLNHLQTKVSPTDYSTWLRPLQASSENGELTLY
                     AQNQFVENWVKDKFLSEITELARFLAKNDNLVVTLKVGIKPTEQPKVSPNLKNESSEF
                     SNTGFKTGLNPNQTFDNFVQGRSNQLAKLVAQQVVDNLGESHCNPLSFYAGTGLGKTH
                     LLHAIGNELVKRTPGTRVLYIHLERFYRDIIKAINSSNNDAEKIKKFYRSLDVLMIDD
                     IQFLANKPKVQEEFLHLLNALFEQRKQIILTSDVLPKNIDKIDTAIKSRLSGGISATI
                     EPPELETRVAILRQKALERGADLQEDAAYLMGQKLRTNVRELEGALNRVIAWQKLTLR
                     PITVDAVRETLQDLFASFDYLITIENIQRIVAEYYGIKVADIKSKSRARSIARPRQMA
                     MALAKELTNHSLPEIGREFGGRDHTTVMHACKTINELRDTESGIQEDYIHLTRKLSS"
     gene            complement(18801..20648)
                     /locus_tag="PXE44_000248"
     CDS             complement(18801..20648)
                     /locus_tag="PXE44_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5587796.1"
                     /translation="MFEKLFKWFEARVETYPNEMPKTPKSGLIPFIYDATKGMRFYIL
                     LLTVLVAAVGIIEAVLFRFMGDLVDWVNKYSPTELWAEKGWTIIGMFFIALLAVVFVY
                     LASSIRFQSLQGVFPMRLRWNFHRLMLGQSLGFYQDEFAGRVSAKVMQTALSVRDVIM
                     TCADMLVYVLVYFTTSSVILLQLDGWLFVPFILWVISLAATIIYFVPKLAQAAQEQSD
                     ARSLMTGRITDAYANIATVKLFSHGNRESAYAKESMEGFMVTVHKQMRLVTVIETVTN
                     LTSMALIISTAGIGLWLWGSSVVTAGAIATSTALALRIKGLSQWIMWEFARLFENLGT
                     VQDGMITLSKPHNVVDKIDAKPLNVTKGEIKFENVDFAYDPKKPLLKDFDLTIKPGEK
                     VGLVGRSGAGKSTLTNLLLRFYDIQDGSITIDGQNIRDITQESLRSQIGLVTQDTSLL
                     HRSVRENLMYGRPNATEEEMIKAVEQAAASEFIPNLQDAKGRTGFDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEVAIQENLTQLMEGKTVVAIAHRLST
                     IMAMDRLIVLDKGEIVEQGTHEELLAQNGVYARLWAHQSGGFLPENTDI"
     gene            complement(20717..21226)
                     /gene="luxS"
                     /locus_tag="PXE44_000249"
     CDS             complement(20717..21226)
                     /gene="luxS"
                     /locus_tag="PXE44_000249"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL5587797.1"
                     /translation="MPLLDSFKVDHTKMNAPAVRVAKTMTTPKGDTITVFDLRFCRPN
                     IDILPVRGIHTMEHLFAGFMRDHLNSEQVEIIDISPMGCRTGFYMSLIGSPSAEEVVK
                     AWTASMEDALHKIPDVSAIPELNEYQCGSYKEHSLKEAHQIARNVLAAGIGINRNEDL
                     ALDEKLLNP"
     gene            complement(21244..23910)
                     /locus_tag="PXE44_000250"
     CDS             complement(21244..23910)
                     /locus_tag="PXE44_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HDL5587798.1"
                     /translation="MTMEENTHTVVDAQIRQPRKISPFWLLPIVAFIIGALLFFQILK
                     EQGETITIRFSKGDGITAGKTAIRYQGLQIGQVKRVYFVDNLKEVEVQAEINPEAKSI
                     LRKQTKFWLVQPSASLAGVSGLDAIVSGNYITLLPGEGDFVDEFIAEEDPPAITVSDG
                     DLLIRLISDDIGSITVGASVYFRKVPVGNIADYRFTKDQKKIEIDVVINQKYAHLVKK
                     ESRFWNISGISADVNLQGIKINMDSLASVVQGAVAFDSPDNMEIAEQGQKFELYPDLK
                     SAKRGIEINVKLPLTHNLKINETGVFHQNVQIGMLSQLKLPQVKDNGVPQQESGQHKQ
                     IDGVLLIDPSYENLLRSQTKILLKEPKFSLNTEQLTKVGELLRGVYFEVHPGEGEPQS
                     EFVVQNEADYLLSLPNLLSFSLIAPQAYGVDTNQGIYYSDVQIGEIVKRSLELDKVRF
                     DVIIYPPYRNLIGSNSKFVAISNIDMSVGLDGLRVHAGSPSEWLKGGVRLINGKNDGS
                     PKKQYALYKDIESAESGMATLDKKATTTLSADSLSGISEGSLVLYRDFQVGEVLKITP
                     KQQKFEVELFIQPNYRHLLTEKSRFWIEPATSVDVSLKGVNITTAPLMRTLKGAISFD
                     DGGSKGNKTLYASKEKATSGNTYLTLIAKDASKLSEGMDIKYMGLTVGQIEKLELQNA
                     KKQIKATAYIQRKYYPLIAKAGSKFNVISPEISTSGFKNLDAALQNYITVDVGSGQAQ
                     NQFVLADTDTVKTTYANGFPIIVETTNANGIQPEAPVLYRGMQVGIVSRLGLSELGDR
                     VLIYVNIQDKYKHLVRTNTQFWQASGYTMDVSLQGVSMNSGTMSQLLNGGIEFSTPYT
                     KVVKPQAQPNRRFFLQRKLPDEAPAWDQGIAE"
     gene            complement(23993..24433)
                     /gene="fur"
                     /locus_tag="PXE44_000251"
     CDS             complement(23993..24433)
                     /gene="fur"
                     /locus_tag="PXE44_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDL5587799.1"
                     /translation="MSEANIKRLKEVGLKVTEPRLTILALMQDIRDEMQHFAAEDIYK
                     ILLEKGSDIGLATVYRVLNQFEEAGILTRHNFDANKAVFELNMNHEHDHIICMDCGKV
                     FEFKDPDMERRQREISEKHGMELTNHSLYLYGKCSNLTNCDEKK"
     gene            complement(24469..24993)
                     /gene="fldA"
                     /locus_tag="PXE44_000252"
     CDS             complement(24469..24993)
                     /gene="fldA"
                     /locus_tag="PXE44_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDL5587800.1"
                     /translation="MAVVGLFYGSDTGNTENISKMIQKKLGAELIDIRDIAKSTKEDI
                     EAYDFLMFGIPTWYYGESQADWDDFMPTLKEIDFNGKVVAIFGCGDQEDYAEYFCDAM
                     GTVRDVVEPNGGVIVGHWPTAGYTFEVSQALVDDDTFVGLCIDEDRQPELTTERVNKW
                     CEQLKEEMCLDQLA"
     gene            complement(24999..25274)
                     /gene="ybfE"
                     /locus_tag="PXE44_000253"
     CDS             complement(24999..25274)
                     /gene="ybfE"
                     /locus_tag="PXE44_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDL5587801.1"
                     /translation="MAKQDSDCITLDLFATVPKVGRPRTNPLDREQQLRINKRNQLKR
                     DKSSGLKRVELKLHSNLVKLLEEQASEQGVSRGQLIETILNNYLTNR"
     gene            25515..26501
                     /locus_tag="PXE44_000254"
     CDS             25515..26501
                     /locus_tag="PXE44_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL5587802.1"
                     /translation="MSNIHLAIAADFTLAEKLLEALANQDLGIENISAVELEPFGEEQ
                     NLRFGAKAVAQYALDEVNWAEFSHVLFAGKMAQAEVLAEAVKAGCVILDLYGITALIG
                     DVPVVVPSVNDENVANLRERNIVALANPQISQLALALKPLLNEPLKQVWVTSLLPSAY
                     FGDEKVKELAGQTARLLNGIPFDENAERIAFDVVPANVQGEEKSLPFSKAFELQIAKV
                     LPNLTACTTFHSVQVPVFYGVSQMVSVQSEYQLDANALSEAWKAENWLNFNENSVITA
                     VKNGENEEQNALEISQLLAKSEQEVQFWTVADEQRFSLAFLAVKLLKLVLAY"
     gene            26598..27893
                     /locus_tag="PXE44_000255"
     CDS             26598..27893
                     /locus_tag="PXE44_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL5587803.1"
                     /translation="MLEKLFQLKAKGSNVKTEIIAGITTFFTMVYIVFVNPSILGVAG
                     MDTQVVFVTTCLIAAFGTIAMGLFSNLPIALAPAMGLNAFFAFVVVQKLGYSWQVGMG
                     AIFLGSVGLFLLTVLQVRYWFMSAIPLGLRVGIGAGIGLFIALIGFKNMGLVVPNPAT
                     LVALGDLHDPKVLMGILGFFIIVVLAAKGIHSGVLISIVIVTALALAFDPAVSFNGIM
                     SMPPSLGAVVGQVDIAGAFDVALLGMIFSFMLVNLFDSSGTLIAVTTKAGFADEQGRF
                     PKMKQALLVDSTAAMAGSYMGTSAISTYIESGSGVSVGGRTGLTAVVVGILFLLTIFF
                     SPLAGLVPAYATAGALVFVGILMASSLIEVKWDDLTEATPAFITTAMMPFTYSITEGI
                     AFGFISYCVMKAFTGRWKEINLSVLVVSLLFIAKFVWVG"
     gene            28013..28381
                     /locus_tag="PXE44_000256"
     CDS             28013..28381
                     /locus_tag="PXE44_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/TusD sulfur relay family protein"
                     /protein_id="HDL5587804.1"
                     /translation="MSNSVQKLLFIINSAPYGNETFFSALRLALQIQEQHKANTEIKL
                     FLMSDAVTAGLAKQNPAEGYHIQQMLEILTAQGANVKLCKTCTNARGITDLPLADGVE
                     IGTLIELADWTIQADKVLNF"
     gene            28381..29574
                     /locus_tag="PXE44_000257"
     CDS             28381..29574
                     /locus_tag="PXE44_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL5587805.1"
                     /translation="MQNIDVVVIGAGAAGLFCAAQLGQAGKKVVVLDSGKKVGRKILM
                     SGGGFCNFTNLDVSAGNYLSQNRHFVKSALSRYTQWDFISLVSHYGISYHEKELGQLF
                     CDESSQQIVDLLQAECNKGKVEILLRQAVISVEKVANFFEISTACGKFQSEHLVIATG
                     GLSMPALGASPFGYQLAEQFGIPVVPPRASLVPFTWKESDKYFAELSGIALPITVSAN
                     GKSFSNQMLFTHRGLSGPAILQISNYWELGETVEIDLLPSQSITDILSELRQSSPKLQ
                     LKTVLSRYLPKKLVEVWLEQKLLTDKVIADLSKKDLTLLEQFIHHWHFLPNGTEGYRT
                     AEVTKGGVDTDYISSKTMEAKNTAGLYFIGEVLDVTGWLGGYNFQWAWSSAFACAEGI
                     IEKSK"
     gene            complement(29645..29863)
                     /gene="infA"
                     /locus_tag="PXE44_000258"
     CDS             complement(29645..29863)
                     /gene="infA"
                     /locus_tag="PXE44_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL5587806.1"
                     /translation="MAKEDCIEMQGTILETLPNTMFRVELENGHIVTAHISGKMRKNY
                     IRILTGDKVTVEMTPYDLSKARIIFRAR"
     gene            complement(29973..30632)
                     /locus_tag="PXE44_000259"
     CDS             complement(29973..30632)
                     /locus_tag="PXE44_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDL5587807.1"
                     /translation="MYISREKITKTLLLAGITLLCCGIVAVILMGVNQFKLGSQLASI
                     NQVSNLSHLLVRQQANIFSFLLVKNAKTDELVDALDSFAKEDFIIDANIYSPNGVLLA
                     QSQNAKDLKVLLDSEQSAKTQQIVEPIFSQQALVGFLRVTFDAEYGQTTKARVEQQFH
                     QLYGEIIILLLTGILLASSINYYFRRKIHHVQISPKPLMSQVKTQTQRFHSRRRWIKR
                     K"
     gene            30718..31581
                     /gene="serB"
                     /locus_tag="PXE44_000260"
     CDS             30718..31581
                     /gene="serB"
                     /locus_tag="PXE44_000260"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HDL5587808.1"
                     /translation="MTNTIFIYSKTLNEREIEQFSQQTAAILLKQAVYLDYKIAFFST
                     NLTACELRETAQKINADISDLTIIPTLNQGGLLLMDMDSTAIKIECIDDIAKLAGTGE
                     VVSAITASAMRGELDFEQSLRKRVGTLENASESILQKVRENLPLMDGFEQMVAILKEH
                     GWKLAIASGGFDYFADYLKQKYGLNFAVSNQLEIIDGKLTGKVLGKVVDAQHKADTLQ
                     NLATTFNIPPNQWVAVGDGANDLPMLKTASLGVALHAKPKVQAQADFVVNFGDLTALC
                     LLLNANHLFQH"
     gene            31597..32088
                     /locus_tag="PXE44_000261"
     CDS             31597..32088
                     /locus_tag="PXE44_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL5587809.1"
                     /translation="MPSFDIASEITMHEVRNAVENANRVLSTRYDFRGVEAVIELNEK
                     NESIKLTTESDFQLEQLIEILIGSCVKRGIEHSSLDIPTESEHHGKLYSKEIKLKQGI
                     ESDMAKKITKLIKDSKIKVQAQIQGEQVRVTGKSRDDLQAAIQLVKGADLGQPFQFNN
                     FRD"
     gene            32173..32349
                     /locus_tag="PXE44_000262"
     CDS             32173..32349
                     /locus_tag="PXE44_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5363 domain-containing protein"
                     /protein_id="HDL5587810.1"
                     /translation="MMSEQTEKKGWFKKALEKYDAFCDELGVNQGACRGCVPVVKFDP
                     EPEKEQPEKSENKT"
     gene            complement(32397..32693)
                     /locus_tag="PXE44_000263"
     CDS             complement(32397..32693)
                     /locus_tag="PXE44_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587811.1"
                     /translation="MKKHHIIILTLASASVVACSSNTPVQVVNGDKNIDYSQPKTIYI
                     SETNTQPPALTKQEKIKPSNATALCFNGSYSTDIQNPCKKDGGVKQYFAHHHAN"
     gene            complement(32770..33738)
                     /gene="mdh"
                     /locus_tag="PXE44_000264"
     CDS             complement(32770..33738)
                     /gene="mdh"
                     /locus_tag="PXE44_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDL5587812.1"
                     /translation="MCQSKKVALLGAAGGIGQSLALLLKLNLPAKSELSLYDISPVTP
                     GIAVDLSHIPTDVKVTGFAGEDPTEALKDADVVVISAGVARKPGMTRADLFNTNATIV
                     HNLVEKAAKVCPKACIAIITNPVNTIIPIAAEVLKKAGVYDKNKLFGVTTLDVIRANT
                     FVAEAKDVNVKYVRVPVIGGHSGTTILPLLSQATVNGLKLEFTQEQIEQLTHRIQNAG
                     TEVVEAKAGGGSATLSMAQAGAEFALGLVRALIGEDVIRYAYVDNANGETSPAFFAYP
                     IRLGTNGVEKVLPIGNLSEFEKDQLEQLIPVLNDEIQLGQTFNKNA"
     gene            33934..34395
                     /gene="argR"
                     /locus_tag="PXE44_000265"
     CDS             33934..34395
                     /gene="argR"
                     /locus_tag="PXE44_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDL5587813.1"
                     /translation="MDKIDSLSEAFKSLLKEEKFGSQSEIVAALQEMGFDNINQSKVS
                     RMLSKFGAVRTRNPKMEMVYQLPVELSVPTTSSPLKNLVVDIDHNDALIVVKTSPGAA
                     QLIARLLDSMGKAEGILGTIAGDDTIFITPVRNVQIEKLVKNVTQLFETSL"
     gene            34445..35341
                     /locus_tag="PXE44_000266"
     CDS             34445..35341
                     /locus_tag="PXE44_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDL5587814.1"
                     /translation="MHIFITGGTGFIGRELIQQLKSENHHFTLLTRDTEAKSDDLQIN
                     YCYSLDELVDLNDFDAVINLAGEPIFDKRWTDEQKQKLIESRVNLTNKLSQLMNASSN
                     PPHTFLSGSAIGIYGDLPPSANFYTESTACYQDCFTNRLCQQWEAAALKAASDKTRVC
                     LIRTGTVFSPEGGALKKILPLYRCGLGGKLGSGKQYWAWISRRDHVQAVIFLLKNANC
                     SGAYNFVAPNPVRNEEFNRILANAVNRPAFFTVPEFALKLGLGERACLVLDNQQLIPE
                     KLLAASFVFQDPILTENVFLNP"
     gene            35386..37584
                     /gene="uvrD"
                     /locus_tag="PXE44_000267"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             35386..37584
                     /gene="uvrD"
                     /locus_tag="PXE44_000267"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDL5587815.1"
                     /translation="MDFSLLLDGLNDKQREAVAAPIGNYLVLAGAGSGKTRVLTHRIA
                     WLIGVENVPESNILAVTFTNKAAAEMRHRIEYTLSQSGDNRLFGMWVGTFHSIANRLL
                     RSHYLDADLPQDFQIMDTEDQQRLLKRLLKLHNIDEKHFPPKHVAWYINAQKDKGKRA
                     KDIDHYNDPNEKKLVEIYQIYQDACDRAGLLDFAELLIRAYELFKTKSTILQRYQQRF
                     QQILIDEFQDTNNIQYDLIRLLAGDVGKVMIVGDDDQSIYGWRGAQVENIQRFLNDYD
                     NAQTIRLEQNYRSTGNILSCANELIANNDNRLGKNLWTDSGDGEPVDIYCAFNELDEA
                     RFVASQIKQWKEDEGDLAECAVLYRSNSQSRVIEEALIQANIPYRIYGGMRFFERQEI
                     KDALAYLRLIANRQDDAAFERVINTPTRGIGDRTLDTLRQITRGRQITLWQAIQVAVQ
                     EEQLSGRATSSLLRFVELINALDQETEQMSLAEQTDFVIKKSGLYEMYKQEKGEKGEV
                     RIENLEELVTATKQFVKPDEAEELTDLTAFLTHASLEAGEAQASQHEDYVELMTLHSA
                     KGLEFPRVFIVGVEEGIFPSGMSFDEGRLQEERRLAYVGITRAKKKLTISYAESRRLY
                     GKEERHVPSRFIAELPEEHIREVRLRGSINRAASFNQANSFAKNPTKTTASFLNDDTW
                     KMGQKVNHAKFGQGTIINVEGSGEATRLQVAFVGNGIKWLIAKMANLERI"
BASE COUNT        11092 a         7238 c         8223 g        11109 t
ORIGIN      
        1 ctaattatgg ttaatttatg ttaagaggga attgaaatct tgatttattt caaattttac
       61 cgcgggaata tgcaaaaaat agggggtttt cctagccaga aggggagctt tttaggtaaa
      121 ataagaagat tatttttagt taaccttttg ttaagaaaag ataaaggttg atcacagatt
      181 tgagaactta atgtggcttt taaaaatagg tgggggacca ttgtttattt ttaagtaaag
      241 ctgcggctca atagaagcga ttgcgttagc gcaatcatat tttttaaccc aaaagaaaag
      301 tagtgcaaac aatatgatta caatcaaaaa aggcttagat cttccgattg cgggaaaacc
      361 tgagcaagta atccataacg gtaataccgt tactgaagtt gctatgcttg gtgaagaata
      421 tgtgggtatg cgtccttcaa tgaaagtgcg tgaaggcgat gtagtaaaga aaggtcaggt
      481 tctttttgaa gataaaaaga atccgggcgt ggtatttact gcccctgcaa gtggtaccgt
      541 aacggcaatt aaccgtggcg agaagcgtgt tcttcaatcg gtagtaatta aagttgaggg
      601 tgatgagcaa attacctttg ctcgttatga agccagccaa ttagcttcat taacttcaga
      661 gcaagtaaaa caaaaccttg tagagtcagg cttatggacg gctttccgta cacgtccgtt
      721 tagcaaagtg cctgcattag atgcagaacc ttcctctatt tttgtcaatg cgatggatac
      781 caatccactt gcagcaaatc cggaagtgat tattgcagaa caccaacaag ctttctttga
      841 tggcttaact gtattaagtc gtttatttga aagtaaaaaa gtgattcatc tctgtggcag
      901 tgcagactca aatattttaa atgcggcaca aagccgtcag atcaataatg tggaaactaa
      961 ccgctttaaa ggtccgcacc ctgcaggtct ttctggtacg catattcact ttattgaccc
     1021 ggtaggtgca actaaacaag tttggtattt aaattaccaa gatgtaattg ctatcggtaa
     1081 attatttacg accggtgagc tatttactga ccgtgtagtc gcattagccg ggccacaagt
     1141 gaaaaatcca cgtttggtgc gtactcgttt aggggcgaat ctttcacaat taacagccaa
     1201 cgaattaaat gcgggtgaaa accgtgtcat ttcaggttct gtgttaagtg gtgctatcgc
     1261 aacgggagta cacgactatt taggtcgtta cgcattacaa gtgtctgctt tattagaagg
     1321 gcgtgagaaa gcgttcttcg gtatgattgc tccttactct gataagtatt caattacgcg
     1381 tactacttta gggcattttg cgaagaaatt atttaacttc acaacagcag ttcacggtag
     1441 tgagcgtgca atggtaccaa caggggctta tgagcgtgta atgccgttgg atatcattcc
     1501 aacactctta ttgcgtgatt tagcagctgg agatacagac tccgctcaag ctttaggctg
     1561 cttagagtta gacgaagaag atttagcgtt atgtactttt gtgtgttccg gtaaaaacaa
     1621 ctggggacca ttattacgtc aagcgttaga taagatcgag aaggacggtt aaaaatgggt
     1681 ttgaaaaatc tttttgaaaa aatggaaccc gctttccata aaggtggaaa gtatgagaaa
     1741 tggtacacgc tatttgaagc tgcctatacg attttctata caccgggtac ggtcactcgt
     1801 aaagatgctc acgtgcgtga tgcgattgac tctaagcgta tgatgttaat tgtatggtta
     1861 gcgttattcc ctgcgatgtt ctacggaatg tacaatgttg gtgcacaggg tattctagcg
     1921 gcactcaaaa tgggaacatt aacggattta gttgctaaca actggcacta cagcttagcc
     1981 gatagtttag gctctgtagc tgatgcaggc tggggaacta aaatgttgct gggggcaacc
     2041 tatttcctac caatctactt aacagtattt gcagtgggcg gtttctggga agtggtgttt
     2101 gcaatggtgc gtaagcacga gatcaacgaa ggtttctttg taacctctat cttactagca
     2161 ttaattgttc ctccaacatt accgttatgg caagcagccc ttgcaactac ctttggtgtt
     2221 gtggtcgcaa aagaagtgtt cggtggcgtg ggtaaaaact ttatgaaccc tgcgttagcc
     2281 ggtcgtgctt tcttgttctt tgcttacccg ggtcaaattt caggtgatgc agtatgggtt
     2341 gccgcagacg gcttctcagg tgcaactgca ctttctcaat gggcagttgg tggtcaaggt
     2401 gcattacagc acgttgcaac cggtgaaacc attacttgga tggatgcgtt cttaggtaat
     2461 attccgggtt ctatcggcga gacctctacc ttaatgttga ttattggtgc tgcaatcatt
     2521 gtatttgccc gaattgcttc ttggcgaatt attgccggtg taatgattgg tatggcggca
     2581 actgcgacat tatttaatgt aatcggttct gataccaacc cattattctc tatgccttgg
     2641 cattggcatt tagtattagg cggttttgcg atcggtatgt tctttatggc aaccgacccg
     2701 gtatcagctg catttaccaa caaaggtaaa tgggcatacg gtatcttaat cggctttatg
     2761 tgtgtattaa tccgtgtggt aaacccagca taccctgaag gtatgatgtt agcgatctta
     2821 tttgctaact tatttgctcc aatctttgac tatttagtag ttcaagggaa tatcaaacgc
     2881 agattagcga gggtagcaaa caatggctaa atttaataaa gatagcgttg gcggaacgct
     2941 aaccgttgta gttttattaa gtttaatttg ttctttgatt gtggcaggtg cggcagtaat
     3001 gcttaaacca acacaagata ttcagaaaca acttgataaa cagaaaaaca tcttacaagc
     3061 ggccggttta ttgcaaaaag ataccaatgt gcaagaaacc tataccaaat ttattgagcc
     3121 gaaaattgtc gatttagcaa caggtgatta cgttgagggt gtaaccggtt ttgatgctcg
     3181 tgcagcagct aaagatcctg ctcaaaatgt agttatcgca cctgaagatg ataaggcgaa
     3241 tatcaaaacg cgtgctaaat atgctgaagt ttatttagta aaagatgaat ctggcaaaac
     3301 gactcaagta gtattaccaa tgcacggtaa cggcttatgg tcaattatgt acggtttcgt
     3361 tgcggtacaa cctgatgcaa ataccgttaa cggtattact tactatgaac aaggtgaaac
     3421 ggcaggctta ggtggcgaaa ttgcaaaccc gaactggcag aaaaactttg taggtaaaaa
     3481 actgtataac gagcaaaacc aagttgcgtt aagagtgggt aaaggggcat ctgcagataa
     3541 agatcacggt gttgatggtt tatctggtgc aacattaacc tcgaacggtg ttgatggttc
     3601 attcaaatac tggtttggtc aaaatggttt tggtccatat ttagcaaaat ttaaagcagc
     3661 ggcaggagct aactaatggc agacaataac aatcttaaaa aattattgtt atcgccaatt
     3721 gtcgataaca acccgatcgc attacagatc ttaggtattt gttctgcgtt agcggtaacg
     3781 actcaattac aaacagcagt agtaatggcg attgcggtaa gtttagttac tgcattctca
     3841 agtatgttca tttcaatgat tcgtaactat attccaaata gtatccgtat cattgtgcaa
     3901 atggcgatta ttgcatcatt agtaattttg gttgaccaaa tcttacgcgc ttatgcttac
     3961 gatctgtcaa aacaattatc ggtattcgta ggccttatta ttaccaactg tatcgtaatg
     4021 gggcgtgcag aagcattcgc gatgaaatca ggcccggttg aaagttttgt tgatggtatc
     4081 ggtaacggtt taggctacgg tgcaatgtta attatcgttg cctttttacg tgaacttatc
     4141 ggttcaggta aaattttcgg tatcaccatt cttgaaactg ttcaaaatgg cggttggtat
     4201 caagcaaacg gtttattctt attagcacca agtgcgttct ttattatcgg atttgtgatc
     4261 tgggcgatta gaacgtggaa accggaacaa gtggagaaat aagatggaac atcttctgag
     4321 tatctttgtt aagtccatat ttattgaaaa tatggcactt tctttcttcc ttggtatgtg
     4381 tacattcctt gcggtgtcta agaaagtttc aactgctttt ggtttaggta ttgcggtaat
     4441 tgtagtactt ggcattgcag ttccagctaa ccaattagtt tatacccacg tattaaaaga
     4501 gggtgcatta gtagaaggtg tggatttaag cttcttaaac tttatcactt tcatcggggt
     4561 tattgcggca cttgttcaaa ttctggaaat gattttagac aagtttttcc cggcacttta
     4621 tagtgcatta ggtatcttct taccacttat caccgtaaac tgtgcgattt ttggtggtgt
     4681 atcgtttatg gtacaacgtg agtataactt tgtagaatct gtggtttatg gcgtaggtgc
     4741 aggtacaggc tggatgttag ccatcgttgc ccttgcaggt ttaaccgaga aaatgaaata
     4801 ttccgacgtg ccggcaggct tacgtggttt aggtattacc tttattacgg taggcttaat
     4861 ggcgttaggc tttatgtcat tctcaggcat tcaattataa ggagcatatc gtggatagta
     4921 attttatttt cggtattatc gcatttacgg ctctggtatt agtgcttgcg gtgatcattc
     4981 ttttcgctaa atcaaaatta gttgattcag gtgatattac catctcaatc aacaacgatc
     5041 cggaaaaagg cattacgcta ccggctggtg gaaaattact cggtgcttta gcaagtaaag
     5101 ggattttcgt ttcgtcagct tgtgggggcg gtggctcttg cggtcaatgt aaagtacaag
     5161 tgaaatcagg cggtggtgaa attcttccaa ctgagttatc gcacatttct aagaaagaag
     5221 cgaaagaagg ctggcgttta gcttgtcagg taaacgtgaa atcttcaatg gacgttgaac
     5281 ttccggaaga gatctttggt gtgaaaaaat gggaatgtac cgttatctca aacgataaca
     5341 aagcaacctt catcaaagag cttaaacttc aaattcctga aggcgaagaa gtaccgttcc
     5401 gtgcgggtgg ttatatccaa attgaagctg aacctcatac cgttcattat aaagacttcg
     5461 atattccaaa agagtaccac gaagactggg ataaatttga cttatggcgt tatacttcaa
     5521 aagtggacga gcatattatt cgtgcatact caatggcttc atatccggaa gagaaaggca
     5581 ttattatgct taacgtgcgt attgctacac ctccaccacg caatccggac gtaccgccgg
     5641 gtcaaatgtc ttcctacatt tggtcattaa aaccgggtga taaagtaaca atttcaggtc
     5701 cattcggtga gttcttcgct aaagataccg atgcggaaat ggtctttatc ggtggtggtg
     5761 caggtatggc accaatgcgt tcacatatct tcgaccaatt aaaacgttta aaatctaaac
     5821 gtaaaatgtc attctggtat ggtgctcgtt ctgagcgtga aatgttctat gtggaagatt
     5881 ttgatggtct tcaagcagaa aacgataact tcaaatggta tgtggcactt tctgatccat
     5941 taccgggcga cagagaagat tacttccgtg gctttattca taacgtgctt tatgagaact
     6001 acctcaaaaa ccacgaagca cctgaagact gcgaatacta tatgtgtggt ccaccggtga
     6061 tgaatgctgc ggtaatcggt atgctgaaga gtttaggtgt agaagacgaa aacatcttat
     6121 tagatgactt tggtggttga taaaccttaa cttctgacta caagcggtca aatttgcaaa
     6181 attgaccgct tgatttaatt tatattatta agttttggta atttaataat gtgaatttta
     6241 aggggaagca aatggaacaa ctggatatta cggtacttga aaatgccttc aattcccttg
     6301 agaaaacgat tgtcagcctt gagaatcaag cgtggtttaa ccggcaagaa gcaattattc
     6361 aagacacttt gattgcagga gcaatccaaa aatttgaatt tgtttacgaa ttgtcgataa
     6421 aaatgctcaa gcgtcagctc aaaaacattg agtcaaatga cactgatatt gatagcaccg
     6481 agttccgtga tattttgcga atggctgctc aatatgggct gattgaatca ccggaagttt
     6541 ggctggatta tcgcaaaatg cgaaatatta cttcgcatac ttatgatcaa aataaagcac
     6601 agcaggttta taacggaatt tcaaactttc tggaaagtac gtatttttta gttaaacagc
     6661 tccaaaaacg caatagctat gattgattta actgagtcac aacgccaaca ggtgttggat
     6721 attttcaaaa aatacttgcc tgatacaccc gtttgggttt ttggctctcg cattaaaggc
     6781 acagccaaag cctattcgga tcttgattta gcattaatca ccaaacaacc gctcacaatt
     6841 cggcaacaaa cagagttaga gcttgcgttt acggattctg acctaccttt cacagtagat
     6901 ctgattgact gggcaagttg caacgaatcg tttaaacaaa ttattttaca gcgatatgaa
     6961 gtgcttattt aatcgctaaa tatgctataa tttccccaat ttttaacttt tgtgacacca
     7021 atgaaaaaat tttttcttct tttaattgct ttttttactt taagtgcttg tgaaaaagcc
     7081 cctgagcaaa ttacgctgtc aggcaaaacc atgggaacga cttacaccgt aaaatatatt
     7141 gataatggtg aagtggagaa tttgatttcg cctgaaaaaa tgaaaaatga gctggatggc
     7201 ttattgattg aggtgaataa tcagatgtcc acctaccaac aagattcgga aattagccgt
     7261 tttaatcaag caaagcaggc aaatcaagcg gtcgaaattt cgcaggattt tgcaaaagtg
     7321 gtacaagagg caatccgttt aaataaagta accgaagggg ctttagatgt aaccgttggt
     7381 ccattggtga acttgtgggg cttcgggccg gataaacgct taaataaagt gccgtctgat
     7441 gaacaaattg cagagcgttc tgcttatgtc ggcattgata aaattttgct tgaaatgagt
     7501 agtaaaccca agctcaccaa gtcagcagct aatctttatc tagatttatc gtctattgca
     7561 aagggtttcg gtgtagataa attagccgag catcttgaaa ctataggtgt gagcaactat
     7621 ttagttgaaa ttggtggcga attacgaggc aaaggaaaaa atttacaagg cttagattgg
     7681 cggattgcaa tcgagaagcc ggtaatgaca caaggtcaag ccgcacaaat taccgtgccg
     7741 ttgcacaatt tggcgatggc aacttcgggc aattatcgta attattttga agatgaaaaa
     7801 ggcaaccgtc tttcacatat tatcgaccca aaacagttac gtccggtaaa ccataattta
     7861 gcctctatca ccgtatttgc cccaactaca atgacggcag acggcttatc aaccggttta
     7921 tttgttttag gagcggaaaa agcattagca gtggcagagc gtgaaaaatt agctattttt
     7981 ttaattgtta aaaacggcga taaatttgag acacaaatgt cgagcgaatt tagcaaactt
     8041 attcaatcac aatagttgga gtgaaaaatg gaaactgtat tattaacctt tggctttttt
     8101 gtagccgtaa tttttgcgat gtcgattggt tttatcgtga aaggcaaaac catcaaaggc
     8161 agctgtggcg gtatcaccgc tttaggtatg aaaaaaatgt gcgactgcga agaaccttgc
     8221 gataacctaa aatctaaaat cgcagacggc acagccgacc ctgaagaagt ggcacgtttt
     8281 aataaagagc cacaatttta tgaagtgaaa taatatgaat agagatattc gctggaaaca
     8341 gcggtttgat aactataaaa aatcagtaag ttaccttcaa gcagaagcag aaaaatacgc
     8401 tgataccgac attgatgtaa ttaaaaaagg aattatccag agttttgaga ttacacacga
     8461 gttagcgtgg aagttaatgc aagatatttt aaaatgggaa ggtgaagttg atatttatgg
     8521 ttcaaaaacg gcttctcgtt tggcttttaa tcgtgggcta atcactcagg gcgaagtttg
     8581 gctagaaatg ataaaaagcc gcaatataac agttcatact gacgatgaaa ctattttaac
     8641 tgaagaattt aataaaatta tcaaggcgta tctgccttta tttattgaat ttgaacagcg
     8701 agtagaaaca ctatgccaaa ctttggacta agcgaacgag cgacgaaaac tattaccgat
     8761 attcttgcga aataccctga agtggagcaa gccattattt acggctcgcg tgctaaaggt
     8821 aattatcgtg aaggctcaga tgtagatctt acttttaaag gaaaagatct tacttacgat
     8881 attgtcaaac gcatttgggg tgatttagac gattccgact cgccctattt atttgacttg
     8941 tcgatttatc atcaattaac caatgccaac tttattgaac atattgatag agtaggaaaa
     9001 gtcttttacc aaagagaact ctaatttctt aataggcaag cggttcattt tgcaaaattt
     9061 tttgcaaatt tcaccgcttg ttgtttttat tattaaccaa tgtgcttcgc cttaaccgaa
     9121 gttacgggag atttatgaca actcaattaa cctcaaaaac ctacgacact catttcccta
     9181 aattaacccc agaacaactt gctgaaaaca gcaagaaaaa agtcattatc ggtatgtccg
     9241 gtggggtgga ttcctcggtt tccgctttta ttcttcaaca gcagggctac caagtggaag
     9301 gcttgtttat gaaaaactgg gaagaagatg atgatacgga ttattgtacc gccgccgccg
     9361 agcttgccga tgctcaagcg gttgccgata aactgggtat gaaactgcat aaaattaatt
     9421 ttgcagcgga atattgggat aatgtatttg aatatttctt ggcagaatac aaggcaggaa
     9481 gaacgccaaa tccggatatt ttgtgtaata aagagatcaa atttaaagcc tttttggaat
     9541 atgcggcaga agatcttggt gcggattata ttgcgaccgg gcattacgtc cgccgtacga
     9601 gcgatgatca agatgcgaaa cttttgcgtg gcttagatga aaacaaagat cagagttact
     9661 ttttatatgc gttaaataaa aagcagatcg ggcagagttt attcccagtg ggtgaaattg
     9721 agaagccgat tgtgcgagcc attgccgaag acctaggatt agcaaccgca aagaaaaaag
     9781 attctaccgg catttgtttt atcggtgagc gtaaatttaa agatttctta gctcgttttc
     9841 taccggctca accgggggaa atccgcaccg ttgagggcaa attagtcggt cgccatgatg
     9901 ggctgatgta tcacacgctt gggcaacgca aaggcttggg tatcggtggg gtgaaagggc
     9961 taagtgaaga tccgttttat gtggtggaga aagacttaat taacaatgta ttgattgtgg
    10021 cacaaggaca cgataattca gccctgcttt caagtggctt aattgcgaaa cagctcactt
    10081 gggtggatat gcagccgttg cgtgaaaatt tacgctgcac ggtaaaaacc cgttatcgtc
    10141 aagcagatat tgcgtgtgaa atccaagtga ttgatgatga aaccattcga gtgatgtttg
    10201 atgaaccgca aattgccgtg acgccggggc agtctgccgt gttttactta ggtgaagttt
    10261 gcttaggcgg tggcattatt gagtcacaat taaaatagtt ataaataaat attgaaatat
    10321 tttgcatatt tatgcaaaat aagcgaaaag ttaaggcggt aaaacgcctt tttcattttg
    10381 atcgatacaa agaataatat acaaggaaca gactgtggca ctttggggtg gacgctttac
    10441 acaacaagca gacgcaaaat ttaaatattt taacgattca ttacgttttg attaccgctt
    10501 ggcgattcaa gatattgaag gctcaatcgg ctgggcaaaa gccattactt cggtaggtat
    10561 tttaacggta gcagaattag aacaactaat cgcagcatta aaagaggtga gagcagaggt
    10621 agaatctaac cttaccatca tcttaaaaga tgatgcggaa gacattcaca gctgggttga
    10681 atctaagctg attgaaaaag tgggcgattt aggtaaaaaa ttgcacaccg ggcgtagccg
    10741 taatgaccaa gtagcggtgg atattaaaat gtggtgcaag gtgcaagcgg tggcattgca
    10801 agaaagaatc cgagctttgc aagagcgttt ggtttccgtt gccgaagaaa atcaagagtc
    10861 ggtgatgccg ggctataccc atttacaacg agctcagcct gtgaccttcg ctcattggtg
    10921 tatggcgtat tatgaaatgc tagaacggga tttcagccgt ttgagtgatg ctaataaacg
    10981 tatgagcact tgcccgcttg gttcaggggc attggctggt acggcttacg gcattgatcg
    11041 tgacttatta gcccgtgatt taggctttga agaagccaca cgcaacagtt tagacagcgt
    11101 gtcagatcgt gaccatattt tagagctgct ctctactgcc tcgatcagta tgatgcacct
    11161 ctctcgcttt gctgaagatt taatcatttt taatagtggc gaatcaggct tccttgaaat
    11221 gtcggatcgt gtgacctcag gctcatcact tatgccacag aagaaaaacc ctgatgcttg
    11281 tgagctgatt cgtgggaaat ccggtcgggt atttggggca ttaaacggtt tattaactac
    11341 actcaaaggg ttaccacttg cttataataa agatatgcaa gaggacaaag agggtatttt
    11401 tgatgcgttg gaaacttggc aggcttgttt agaaattgca gaacttgtac tggttgatat
    11461 tcaagtgaat accgaacgca cccgagaagc cgctcagcaa ggttatgcga atgcgaccga
    11521 gttggcagat tatttagtgg cgaaaggcgt gccattccgt gaagctcatc atattgttgg
    11581 cgaagcggtg gtttacgcaa tcagcaaaaa agagccactt gaagcattaa gcattaacga
    11641 attccagcaa ttccatcatt cgattggcga tgatgtttac ccaattctct cgttagaatc
    11701 ttgcttagaa aaacgttgtg ccaaaggtgg agtgaaccca caacgtgtgg cagaggcgat
    11761 tgcggtagcc aaagccaatt tagcttcaaa gtaaaaccgg atacaagcgg tcatattttg
    11821 caaaaaaatt gaaaaatatg accgcttgcc gattaaattt tcaatttttt acgcattaag
    11881 attgcaaaat aggcattacc tctttaaact tccgaataat ttttgaattt tatttctact
    11941 actttcttta aagagtttta tatgacggaa tcagttaatt ctactcaagc tggtacagca
    12001 ctttctttag ggcaacaatt aaaagcggct cgtgaagcgt taaatctctc tattacagat
    12061 gttgctcaaa aaaccaactt aaaaaaatca catattgaat cacttgaaaa tgatatcttt
    12121 attctacaaa atgttgcccc aacttttgtg cgtggctatg tgcgtaatta tgtgaaattt
    12181 ttgcgtttac ctgaatccct tatttcgagc gttaattatg gtgaagtcac tattccaaaa
    12241 gaagtcacta aagtttcttc agtaaaacct tcatcaaatt cacaaggtaa aaccttaaag
    12301 tatttaacgg tattagtatt gcttgcggca ttaggaatga cattactttg gtggtggcaa
    12361 gggtatcaaa aagaacagga aaatcgcgag caatttgtta gctctgcacc agttgctgaa
    12421 gtggcgaata ccgctacaaa taatgatgct tcagcacaaa cttccgtgcc tgttcagcaa
    12481 cctattcagg tttcagagca gcctgctcaa gtagcagaaa cggtagttgc agcagaaccg
    12541 gcattaactg aaaaagtaga gcctacacaa gcagcatcat cacctgtaac acaagaagat
    12601 gttaaagtgg tagaagaagt taaacaagct gatgccacca ctgcacaggt tgaggcagtt
    12661 aatgttttac agcaatctca atcggaacaa gcccctacag aacagcctgt tgcggtaacg
    12721 aatgatgagc ttcgtatcga aattaccggt tcacaaagct ggattacagt gcggggtgct
    12781 aaaaataaac gtttagcaga aaaattatat aacaatggtg agactttaag tttcaatgag
    12841 aatgagcaat atcgtttaac cattggtgcg cctgccaatg taaaagtgta ctataaaggt
    12901 caagaagtac cattgaaaat tgatgggcgt gtagctcgta ttcatttacc gttacagtaa
    12961 gcttgattta acgatgaaac gcacgtaaag ctagcattta acgatagctg tctttacgag
    13021 cgttttctgt ttttataaat tttcacaatt agctaaaaat atgatacatc aatcacctat
    13081 taaacgtcgg gtatcgaaga aaatctatgt gggtaatgtg cctgtaggcg gcgatgcacc
    13141 gattacggtt cagtcaatga ccaacacccg cacaaccgat gtggaagcca ccgttgctca
    13201 aattaaatcg ctggaacggg tcggggctga tattgtgcgt gtgtctgtgc cgacaatgga
    13261 cgctgctgag gcatttaaag cgattaaaca acaagtgagt gtgccattgg tggcggatat
    13321 ccacttcgat taccgcattg cgttgaaagt tgccgaatat ggggtggatt gcttgcggat
    13381 taacccgggt aatatcggga atgaagagcg gatccgcacg gtggtagatt gtgccagaga
    13441 taaaaacatt ccgatccgaa tcggggtaaa tgccggatca ttagagcgtg atttgcagga
    13501 aaaatatggc gagccgacac cacaggcatt attagaatcg gcattacgcc acgttgatca
    13561 cttagatcgc cttaactttg atcaattcaa agtcagcgtg aaagcctcag atgtatttct
    13621 agcggttgaa tcttaccgac ttcttgcaaa acagattgat cagcccctgc atttaggtat
    13681 tactgaagca ggcggagcga gagccggttc ggtgaaatcg gcggtaggct taggtctgtt
    13741 gctagcggaa gggattggtg ataccttgcg tgtgtcatta gcggctgatc ctgttgaaga
    13801 ggtgaaagtc ggttttgata ttttgaaatc gctgcgtatt cgctctcgtg ggatcaactt
    13861 tattgcttgc ccaacctgct cccgccaaga aattgatgtg attgcgacgg taaacgagct
    13921 agaacaacgt ttagaagaca ttatcacgcc gatggacgtt tcgattatcg gctgtgtggt
    13981 aaacggtcca ggagaggcgt tggtgtctga tttaggggta accggcagca ataaaatgag
    14041 cggcttttat ttagatggcg ttcgtcaaaa agagcgtttt gacaacgcaa aattaatcga
    14101 ccaattagaa gctaaaattc gtgccagagt tgcacaacaa cataatcgta tagatattga
    14161 acaaatagga taaatatagt ggcaaaaaca attcaagcaa ttcgaggaat gaacgactgc
    14221 tcaccaagtg agtctccatt atggcaatgg gttgaacaga aagtgcgtaa tgtgctggca
    14281 agttacggct attcggaagt acgtatgccg attgtagaaa gcactccgtt atttgcccgt
    14341 gccattggcg aagtaaccga tgtggtgtcg aaagagatgt acacgttctg ggataacgat
    14401 gagcaattaa cgctccgccc ggaaggtaca gccggttgtg tgcgtgcagc gattgagcgt
    14461 ggttggatct acaataatga gcaacgctta tggtatatgg ggccgatgtt ccgccacgaa
    14521 cgtccacaga aagggcgtta ccgccaattc caccaagccg gtgttgaagt gtttggcatt
    14581 gcaaatccgg aaattgatgc ggagttgatt attctcaccg cacgcctatg gaaagagtta
    14641 ggcatcgatc aacatgtttc tttgcaactt aactctatcg gctcgttaga agctcgtgca
    14701 aattatcgct cagcattagt aaaattttta gaaaatcaca ccgctttaat gaacgaagaa
    14761 gagaaagagc gtttggtgaa aaacccgcta cggattttag ataccaaaaa cgaagcacta
    14821 caaaatgtat taaacgatgc cccaaaactg ctcgattatt tagatgatga cagccgtgaa
    14881 cattttaccc aactttgtgc cttacttgat gcaatgggaa tccgttacga agtcaatcca
    14941 aaattggtgc gtggtttaga ctattacaat aaaaccgtgt ttgaatgggt cacttcagcc
    15001 ttaggctcac aaggcaccgt atgtggcggt gggcgttatg acggcttagt cgaacaactc
    15061 ggtggacacg caacccaagg cgttggcttt gcaatgggct tagagcgttt agtgctgttg
    15121 gtacaagagg tgaataaaga gattgattta ccaaaaacgg tggatgtgta tatcgtattc
    15181 tccggtgaag gggcaacact aaatgccttc caacttgcag agcagattcg tagcgaattg
    15241 ccacacgttg gcgtaatgac acactgttcc ggcggtaaat tccaaaaaca atttaagcgt
    15301 gcagataaag tagaagcgca aattgcgtta gtgattggcg aaagcgaagt ggctgaaaag
    15361 caagttgtta tcaaagattt acgttcagga gcagaacaaa tcacgattgc tcaatcagag
    15421 ctgattgctg agctatctaa acgattttag gagaaaaaga tgagtaacga atatttaaat
    15481 aaaaccgaag agcagcaatt tgaagatgcc aaaaattggt ttaaagagaa cgggacaccg
    15541 attttggtgg cgattatttt agcttgtgcg gcaactgccg gctggaattt ttggaaaaat
    15601 caccagcttg aaacggcaca aaaaacatcc gcttcatacc aaagcgtgat ggagagttat
    15661 ttgcaaaatc caagcaaaaa tcaaccgctt gttgagaaat ttattgccga taataaagat
    15721 tcaagctatg ccgcttttgc ccaatttgaa gtagctaaac aagcggtcga aaaagggaat
    15781 ttttctgcgg caaaaacagc gttacagtca gttttgactt attcggattc aaccttacaa
    15841 tctatcgctc gttttcgttt ggctcaaatt gacttccaac taaaatctta cgatgaagca
    15901 ctggcaagtc tggctcaaat tcaagataaa gcgtgggaat tgcgtaagca aattctaaca
    15961 ggcgatattc ttgcagcaaa aggcgatatt cctgcggcga aaagtgctta tgagcaagct
    16021 aaaacgaccg caaatcctca ggaacaaacc ttaattgatg tacgtttgaa taacttataa
    16081 aaacaaagcc tgattgaaat aaaatcaatc aggctttttt gttggtttag tgttgctgtg
    16141 aattataaac gcattggcat aatcacatat tcggcagagc tgttgtcgca atcttcaatt
    16201 aagcaactag aacttgaatc aactaagcta aaacgcacac gttggcattt taaggtgttt
    16261 aacacatcta atacatagct aacgttaaag ccgacttcca tttccgggct gtcgtaggca
    16321 acatcaatga tctcttccgc ctcttcttgc tccggattgt ttgcggtaat tttgagtaaa
    16381 ttattgctta aggttaaacg cacccctcta aatttctcat tagaaagaat cgctgcacgc
    16441 attaatgccc gtttaagggt ttcggtttcc gcttctagaa tgcgatctgc attgcgaggc
    16501 agaactcggc ggtaatccgg gaatctgcca tcaatcagtt tagaggtaaa tacaatgccg
    16561 ttcattgtaa tacggatatt gcttgaaccg atttccaaac gcacggtttc atcgttgctg
    16621 gcaaccaagc gggaaagctc aagtacggct ttgcgtggca cgataaccga atgagtcaaa
    16681 agctcttgat ttagcaccat tgtgcaaacc gctaaacggt gcccatctgt ggcaacggtg
    16741 cgtaataaat tgccttcggt ttcaaatttc attccgttta agaaataacg ggcgtcttgg
    16801 tttgccattg agaattgggt ggcatcaatc agacgggcaa gggtagattg atcaatcgag
    16861 aaatccactt ccggtttcca atccatcaaa ttcgggtaat cacttgccgg taatgtagct
    16921 aaactaaatt tactacgtcc tgctttgata atggctttat cttcttcaaa ttgtaccgaa
    16981 atcacgctgt catcaggtag gcttttactg atatctagga attttttagc tggaatggta
    17041 aattttccgg caaaatcgac cgcttgtagc agttgtgctt gggtggttaa ttccacttct
    17101 aaatcggtac cggttaaaga tagacgatta ccttcaattt ctaataaaag attgttaata
    17161 acaggcaaag tcggacggct acttaatacg ccacacacct gttgtaaagg cttaagtaat
    17221 tgttctctgg taatggtaat ttgcataatc attccttcat cgggctaaga tgataatttg
    17281 cgtgttaaat ggatataatc ttcttggata ccgctttcgg tatcgcgtaa ttcattaatg
    17341 gttttgcagg cgtgcatcac ggtagtatga tcccgaccgc caaattcccg accaatttcc
    17401 ggcaagctgt ggttggttag ctcttttgcc agtgccattg ccatttggcg aggacgggca
    17461 atgcttcttg cccgactttt cgatttaata tcggcaactt ttatgccgta atattcggcc
    17521 acaatgcgtt gaatattttc aatcgtaatc aagtaatcaa aagaggcaaa cagatcttgc
    17581 aaggtttcac gcaccgcatc aaccgtaatc gggcgaaggg tcaatttttg ccacgcaatc
    17641 acacggttta atgctccctc caattcacgc acattggttc ggagtttttg ccccattaaa
    17701 taggcggcat cttcttgcaa atctgctcca cgttctaagg ctttttggcg aagaattgcc
    17761 actcgggttt ccaattcagg tggctcaatg gtcgcactaa taccgccgct taaacgggat
    17821 ttaatcgctg tgtcaatttt atcgatattt tttggcaaaa cgtcagaggt tagaataatc
    17881 tgctttctct gctcaaataa agcattgagt agatgtaaaa actcttcttg tactttcggt
    17941 ttattggcaa gaaattgaat atcgtcaatc attaacacat caagcgagcg gtagaatttt
    18001 ttgatttttt ccgcatcgtt attggaactg ttgatcgcct taataatatc acgataaaaa
    18061 cgttccaaat gaatatatag cacccgagta cccggtgtgc gtttaactaa ttcattgccg
    18121 atagcgtgta ataagtgggt tttgcccaaa ccggtgccgg cataaaacga gagtgggttg
    18181 caatggcttt cgcctaaatt atccaccact tgttgagcca ctaatttagc taattggttt
    18241 gagcgacctt gtacaaaatt atcaaaggtt tgatttggat ttagcccggt tttaaagccg
    18301 gtattgctga attctgaact ctcgtttttt agatttgggc tgacctttgg ctgttctgtc
    18361 ggtttaatcc caacttttaa cgtaaccacc aaattatcat ttttcgccaa aaaacgggca
    18421 agctcggtaa tttccgataa aaatttatct tttacccagt tttcaacaaa ttgattttgt
    18481 gcataaagtg taagttcccc attctcagaa ctcgcttgca gaggacgcag ccaagtgctg
    18541 taatcagttg gtgagacttt cgtttgtaag tgattaaggc aatcagacca aagggaagac
    18601 acaatttgct ccaaaattat tgttattaaa ggtcataatg atacctgaaa aaaggggaga
    18661 aatcttgcaa atttttgaga gattttatgg gggcttggaa gcgattttaa ttggcgaaca
    18721 agcggtcgtt ttttgttgaa atcttgcaaa ttgctaacaa aaaatgaccg cttgcttagt
    18781 atgatgtgtg atggagttga ttaaatatcc gtattctccg gcaagaaccc accgctttgg
    18841 tgagcccaaa gtctggcgta aacaccgttt tgagcaagca attcttcgtg cgtgccttgt
    18901 tcaacgattt cgcctttatc taacacgata agtctgtcca ttgccataat ggtagagagg
    18961 cggtgggcaa tcgcaaccac ggttttacct tccattaatt gcgtgagatt ttcttgaatt
    19021 gccacttcta cttcagagtc taacgcactg gttgcctcat cgagtaataa aatcggagcg
    19081 tcttttagca tcactcgggc aatcgcaatc cgttggcgtt gtccgccgga aagttttacc
    19141 cctcgttcgc caacgtgtgc atcaaaaccg gttctgcctt tggcatcttg caggtttgga
    19201 ataaattcac tggcggcagc ttgttcaacg gctttgatca tctcttcttc agtggcgttt
    19261 gggcgaccgt acatcaagtt ttcacgcaca gagcggtgta acagcgaggt gtcttgagtt
    19321 actaaaccaa tttgtgagcg aaggctttct tgggtaatat cacggatatt ttgcccgtca
    19381 atcgtgattg aaccgtcttg aatatcgtaa aaacgcaata ataaattggt gagcgttgat
    19441 ttacctgcac cactacgccc gactaagccg actttttccc ccggttttat agtgaggtca
    19501 aaatctttca gtagcggttt tttcggatcg taggcaaaat ccacattctc aaatttaatt
    19561 tcgcctttgg ttacatttaa cggtttcgca tcgattttat ctaccacatt gtgtggtttg
    19621 gaaagcgtaa tcataccgtc ttgtaccgta cctaaatttt caaatagacg agcaaattcc
    19681 cacataatcc attgtgataa gcctttaatg cgtaaagcca aagcggttga ggtggcaatc
    19741 gcccctgcag ttactacgct tgaaccccaa agccataaac cgatacccgc agttgaaatt
    19801 attaatgcca tcgaggttag attagttacg gtttcaatta cagtcactaa acgcatctgt
    19861 ttatgcactg tcaccataaa tccttccatc gactctttcg cataggcaga ttcacggttg
    19921 ccgtgcgaga agagtttgac ggtggcaatg ttagcgtaag catcggtaat tcgaccggtc
    19981 attaaacttc tggcatccga ttgctcttga gcagcttggg ctaatttcgg gacaaagtag
    20041 ataatggttg ccgctaatga gatgacccat aaaataaacg gcacgaataa ccagccgtca
    20101 agttgtaata aaatcacgct ggaggtggtg aagtaaacca gcacatacac caacatatcg
    20161 gcacaagtca taatcacatc acgcacggaa agggcggttt gcattacttt tgcagacact
    20221 cttcctgcaa attcgtcttg atagaaacct aaactttgcc caagcattaa gcggtggaag
    20281 ttccaacgca aacgcattgg aaatacccct tgcagagatt gaaagcgaat gctgctggca
    20341 aggtaaacaa acaccactgc aagtagggca ataaaaaaca taccgataat cgtccagcct
    20401 ttttctgccc aaagttctgt tggcgagtat ttattgaccc aatcgactaa atcgcccata
    20461 aagcgaaaca gaaccgcttc gataataccg acagctgcaa ctaatacggt cagtagtaag
    20521 atataaaacc gcattccttt agtggcatcg taaataaaag gaatgagacc tgattttgga
    20581 gttttcggca tttcatttgg ataggtttcc acccttgcct caaaccattt aaacagtttt
    20641 tcaaacataa atttgtgatt tccgaataaa aatgcagggg caaatagcca tttgcccccg
    20701 aaaaaatgtg aaaaaattat ggattaagta atttttcgtc taaggctaaa tcttcgttgc
    20761 gatttatacc aatgccggca gctaatacgt tgcgggcaat ttgatgtgct tcttttaaag
    20821 aatgctcttt gtaagagcca cattgatatt cattcagctc agggattgcc gatacatcag
    20881 gaattttatg taacgcatct tccattgatg ccgtccaagc tttgacaact tcttctgctg
    20941 aaggcgatcc aatcagtgac atataaaaac cggtacgaca acccattggt gaaatatcaa
    21001 tgatctccac ttgttcgcta ttgaggtgat cacgcataaa accggcaaaa agatgttcca
    21061 tcgtgtgaat cccacgcacc ggtagaatat caatattcgg acgacagaaa cggagatcga
    21121 acacggtaat ggtatcgcct ttcggtgtgg tcattgtttt tgcaacacgc acggctggtg
    21181 cgttcatttt ggtgtgatct actttaaaac tatctaataa aggcattttt tactccatca
    21241 atattattcc gcaattcctt gatcccaagc cggggcttca tcaggtagtt tgcgctgtaa
    21301 gaaaaatcgt ctgtttggct gggcttgagg cttcaccact ttagtgtaag gggtagagaa
    21361 ctcaataccg ccatttaata actgcgacat tgtgccggag ttcatactta caccttgcag
    21421 gctcacgtcc atagtgtaac ctgaagcttg ccagaattgg gtattggttc tgactaagtg
    21481 cttgtattta tcttgaatat tgacataaat aagcactcga tcgcctaact cacttagccc
    21541 taaacggctg actattccca cctgcattcc acggtaaagc accggtgctt ccggctgaat
    21601 accgttagca ttggtggttt cgacaataat ggggaaaccg ttggcatagg tggtttttac
    21661 cgtatcggta tctgcaagaa caaattggtt ttgagcttgc cctgaaccga catcaaccgt
    21721 aatgtaattt tgtagtgctg catctaaatt tttaaagccg gaggtactga tttcaggcga
    21781 gataacatta aatttactgc cggcttttgc aattaacgga tagtatttcc tttgaatgta
    21841 agcggtcgct tttatctgtt tttttgcatt ttgtagctca agtttttcaa tttgacctac
    21901 ggttaagccc atatatttaa tgtccattcc ttcgcttaac ttactcgcat cttttgcaat
    21961 cagcgtgaga taagtattgc ccgaagtggc tttctcctta cttgcataaa gcgttttatt
    22021 gccttttgaa ccgccatcat caaagctgat cgcccctttt aaggtacgca ttaatggagc
    22081 tgtagtgata ttcacgcctt tgagtgacac atcaacactc gttgccggct caatccagaa
    22141 acggcttttc tcggttaata gatgacgata atttggctgg ataaatagct caacctcaaa
    22201 tttttgttgt ttcggggtta tttttaacac ctcgccgacc tgaaaatcac gatatagcac
    22261 caaagagcct tcgctgatac cagataatga atctgccgaa agggtagtgg tggctttttt
    22321 atctaaggta gccatgcctg attccgcact ttctatatct ttataaagtg catattgttt
    22381 tttcgggcta ccgtcatttt taccgttaat taagcgaaca ccgcctttta accattcaga
    22441 tggtgaaccg gcgtgaactc ttaacccatc tagccccacc gacatatcaa tgttggatat
    22501 cgcaacaaat ttgctgttac tcccaatcag gttgcgataa ggcggataaa taatgacatc
    22561 aaaacgtact ttgtctaact ctaggctacg tttgacgatt tctccgattt gcacatcgct
    22621 atagtaaatc ccctgattgg tatctacccc ataggcttga ggagcaatta agctaaagct
    22681 aagtaaattc ggtaaactta atagataatc ggcttcgttt tgtaccacaa attccgattg
    22741 aggttctcct tcccccgggt gaacctcaaa atagacacca cgcagcagct cacctacttt
    22801 cgtcagctgc tctgtgttta aggagaattt cggctctttg agcaagattt tagtttggga
    22861 acgtagtaaa ttttcataac ttgggtcaat taataaaacc ccatcaatct gtttatgctg
    22921 ccctgattct tgctgtggca caccgttgtc ttttacttgc ggtaatttta gctgcgaaag
    22981 cataccaatt tgcacatttt ggtgaaaaac accagtctca ttaattttta aattatgtgt
    23041 aagcggtagt tttacgttga tttcaattcc acgtttagct gatttcaaat ccggatatag
    23101 ctcaaatttc tgtccttgct cggcaatttc catattatca ggcgaatcaa aagcaaccgc
    23161 accttgtact accgaggcaa ggctgtccat attgatttta ataccttgta aatttacatc
    23221 agcgctaata ccgctaatat tccaaaatcg gctctctttt tttactaaat gagcgtattt
    23281 ttgattaatg acgacatcaa tttcgatttt tttctggtct ttggtaaaac gataatcggc
    23341 aatattaccc actggcactt tacggaaata aacactcgct ccgacagtaa tagagcctat
    23401 gtcatcagag attaaacgga tcagtaaatc gccatcgctc acggtaatag ccggaggatc
    23461 ttcttctgca ataaattcat caacgaaatc gccttcaccg ggaagtaggg taatatagtt
    23521 accagaaaca atcgcatcta agcctgaaac gccagccaaa gaagcacttg gttgcactaa
    23581 ccaaaattta gtctgtttgc gtaaaatact tttggcttca gggtttattt ctgcttgcac
    23641 ttccacttct tttaaattat cgacaaaata aacacgctta acctgaccaa tctgcaagcc
    23701 ttggtaacga atagcggttt ttccggcagt aatgccatca cctttactaa atcgaatcgt
    23761 gattgtttca ccttgctctt ttaaaatttg gaaaaatagc aaagcaccaa taataaaagc
    23821 aactattggt aacagccaaa aaggagaaat tttgcgaggc tgccggattt gggcatctac
    23881 aacagtgtgg gtattttctt ccatcgtcat tttaaattag ggaaacctat atagaacaat
    23941 accgaagacc caaagggact tcggtaatag aaataagtca aaataagaaa tattattttt
    24001 tctcatcaca gttggttaaa ttactgcatt taccatacag atataaactg tggttagtca
    24061 attccattcc gtgtttttca ctgatttcac gttggcgacg ctccatatca ggatctttaa
    24121 attcgaacac cttaccacaa tccatacaaa taatatggtc gtgttcgtgg ttcatattta
    24181 attcgaatac tgctttattc gcatcgaaat tatggcgggt taaaatgccg gcttcttcaa
    24241 attggtttaa gacacgataa accgttgcta aaccaatgtc tgagcctttt tctaacagaa
    24301 tcttataaat atcttctgct gcaaaatgtt gcatttcatc acgaatatct tgcattaatg
    24361 caaggatagt caaacgaggc tcggttactt ttaagcctac ctcttttaaa cgtttaatat
    24421 ttgcttcaga catatatgcc acttatttat ctttatccaa aatagttatt atgcgagttg
    24481 gtctaagcac atttcttctt tcaactgctc gcaccattta ttaacacgtt cagtggttaa
    24541 ttcaggctga cgatcttcat caatacataa gccgacaaaa gtatcatcat caactaaggc
    24601 ctgagaaacc tcgaaagtgt aaccggcagt tggccaatga ccaacaatca caccaccatt
    24661 tggctctacc acatcacgca cagtacccat tgcatcacag aaatattctg cataatcttc
    24721 ctgatcaccg caaccaaaaa ttgcaacgac tttgccgtta aaatcaatct cttttagggt
    24781 aggcataaaa tcgtcccaat cggcttgcga ttcaccataa taccaagttg ggatcccaaa
    24841 catcagaaaa tcgtatgctt caatatcttc ttttgtgctt ttggcaatgt ctcgaatatc
    24901 aattaattca gcccctaatt ttttctgaat catttttgaa atattttctg tattgcctgt
    24961 atcgctaccg taaaataaac ctacaactgc cattgctact acctatttgt gagataatta
    25021 tttagaattg tttcaattaa ctgcccgcgt gaaacacctt gttcagacgc ctgttcctct
    25081 aataacttta ctaaattcga atgcagtttt aattctaccc gttttaaacc ggaagattta
    25141 tctcgtttta gttggttacg tttattgatt cgaagttgtt gttctctatc aagaggattt
    25201 gtacgaggac gcccaacttt tggtacagtc gcaaacagat ctaaggttat acaatctgaa
    25261 tcttgttttg ccattttgtt ttactattgg gctttagcta atcctcagtt ttaatgagaa
    25321 tgataagcta cttttaaagt gtgaactata tcaaaagttc aatcatttga aaagtaaatt
    25381 acaggcaaaa caagcggtgt ttttaggttt aaaatttgca aaaaccataa attacacatt
    25441 atgagttatg atttttctga agcgtgaaat tagcttataa tgaaaccgaa taacgttatg
    25501 aaaaaggaga tagtgtgtcg aatatccatt tagcgattgc cgctgatttt acgctggcag
    25561 aaaaattact tgaagcatta gcaaatcaag atttagggat tgaaaatatt tcagccgttg
    25621 agcttgagcc gtttggtgaa gagcagaatt tacgctttgg ggcaaaagca gtcgcacaat
    25681 atgcgttaga tgaagtaaat tgggcggaat ttagccatgt tctatttgcc ggcaaaatgg
    25741 cacaagcgga agtgttggca gaagcggtga aagccggctg tgtgatttta gatttatacg
    25801 gcattaccgc actcatcggc gatgtaccgg tagttgtgcc aagtgtaaat gatgaaaatg
    25861 tcgcaaattt gcgtgagcga aatatcgttg ctttagccaa tccgcaaatt tcacaactgg
    25921 cgttagcctt aaagccattg ttgaacgagc cgttaaaaca ggtgtgggta acctcacttt
    25981 taccgtctgc ctattttggc gatgaaaaag tcaaggagct tgccggacaa actgcccgtt
    26041 tgttaaacgg cattccgttt gatgaaaatg cggaacgcat tgcctttgat gttgtgccgg
    26101 ctaatgtgca aggagaagag aaaagtctgc cgttctcaaa agcctttgaa ctgcaaattg
    26161 caaaagtttt accaaatttg accgcttgta ctaccttcca cagcgtgcaa gtgccggtct
    26221 tttatggggt ttcccaaatg gtgtcggtgc agtctgaata tcagttagat gctaatgcgt
    26281 taagtgaagc ttggaaagcc gaaaattggc tgaattttaa tgaaaattca gtgattacag
    26341 cggtgaaaaa tggcgaaaac gaagagcaaa atgcgttaga aatcagtcag ttattggcga
    26401 aatccgaaca ggaagtgcag ttctggacgg tcgccgatga acagcgtttc agtctggcat
    26461 ttttagcggt gaaattacta aaattagttt tagcgtatta agtgcttgca aaagtattta
    26521 ttccgtgata aaaatcgtca acgcccgagc aaccgattgc ttgggcgttg tcattttgtt
    26581 taaacaccga aggtcttatg ttagaaaaat tatttcagct caaagcaaaa ggcagtaatg
    26641 taaaaaccga aattatcgcc ggtattacta cctttttcac aatggtttat attgttttcg
    26701 tcaatccgtc tattttaggc gtggcgggaa tggacaccca agtagtgttc gtgactactt
    26761 gtttaattgc cgcttttggt actatcgcaa tggggttatt cagtaattta ccgattgctc
    26821 ttgcaccggc aatgggctta aatgcctttt ttgcattcgt agtggtgcaa aaattaggtt
    26881 actcgtggca agtgggtatg ggggcgattt tcttaggctc tgtcggctta ttcctattaa
    26941 ctgtattaca agttcgttac tggtttatgt ctgcgattcc acttggttta cgtgtaggta
    27001 ttggtgccgg tatcggttta ttcattgccc taatcggttt taaaaatatg ggcttggtgg
    27061 tgccaaatcc ggcaacgctt gtggcactag gtgatttgca cgacccgaaa gtgttaatgg
    27121 ggattttagg ctttttcatc attgttgtat tagcggcgaa aggtattcac tcaggcgtgt
    27181 taatttcaat tgtgattgtt accgctcttg ccttagcctt tgacccggcg gtgagcttta
    27241 acggcataat gtctatgcca ccaagccttg gggcagttgt ggggcaggtt gatattgccg
    27301 gtgcttttga tgttgcctta ctcgggatga ttttctcctt tatgttggta aacctgtttg
    27361 actcttccgg caccttaatt gcggtgacta ccaaagcagg ctttgcggat gaacaagggc
    27421 gtttcccgaa aatgaaacaa gctctgctag tagatagtac cgctgcaatg gcaggctctt
    27481 atatgggaac ttcagcgatt tctacctata ttgaaagtgg ctccggcgtt tcagttggcg
    27541 gtcgtaccgg cttaactgcg gtggtagttg ggatcttatt cctattaacc attttcttct
    27601 caccacttgc agggttagta cctgcctatg caactgcggg ggcattggta tttgtaggaa
    27661 ttttaatggc atctagctta attgaagtaa aatgggacga tttaactgaa gctacgccgg
    27721 catttattac taccgcaatg atgccgttta cttactccat cactgaaggg attgcattcg
    27781 gctttatcag ctactgcgta atgaaagcct ttacagggcg ttggaaagaa atcaaccttt
    27841 ctgttttagt ggtttcactt ctctttattg ctaaatttgt gtgggtgggt taagctctac
    27901 ccccctctcc ctgatttttc ttctaaacga agaaaaatct atccctctcc cacaaagggg
    27961 agagggtatt tgattcatta tctatgtgtt tcgtgtattc cgaggtaaaa caatgtctaa
    28021 ttcagttcaa aaacttcttt ttatcatcaa ttctgctcct tacggcaatg aaacgttttt
    28081 cagtgcgttg cgtttggcat tacaaattca agaacaacat aaagccaata cggaaattaa
    28141 acttttctta atgtcggatg cagtcaccgc agggctggca aagcaaaatc cggcagaggg
    28201 ttatcatatt cagcaaatgc tggaaatttt aaccgcccaa ggggcaaacg taaaattatg
    28261 caaaacctgt actaatgctc gagggattac agatttgcct ttagccgatg gggtagagat
    28321 tggcacatta attgaactgg cagattggac aattcaagca gataaggtac tgaattttta
    28381 atgcaaaata ttgatgttgt ggtgattggt gccggtgcgg cagggctgtt ttgtgctgcc
    28441 caactggggc aagctggcaa aaaagtggtg gtgttagaca gcggtaagaa agtcggacgt
    28501 aaaattttaa tgtctggcgg tggtttttgc aattttacga atttagacgt aagtgccgga
    28561 aattacctta gccaaaaccg ccattttgtg aaatccgccc tttcacgtta tacccaatgg
    28621 gattttattt cgcttgtcag ccactatggc attagctatc acgaaaaaga gctagggcag
    28681 ttgttttgtg atgaaagctc gcaacaaatt gtcgatttac tacaagcaga atgcaacaaa
    28741 ggcaaggttg agattctgtt acggcaagcg gtcatttctg tggaaaaagt tgcaaatttt
    28801 tttgaaattt cgaccgcttg tggcaagttc caatccgaac acttagtgat tgcaaccgga
    28861 gggctttcaa tgccggctct cggggcaagc ccttttggct atcaacttgc cgagcagttt
    28921 ggtattcctg ttgtgccacc acgtgcaagc cttgtgcctt ttacttggaa agagagcgat
    28981 aaatattttg ccgagctttc cggcattgca ctgccgatta ctgtttccgc caatggcaaa
    29041 agttttagca atcagatgtt gtttacgcac agaggcttat ccggcccggc aattttgcag
    29101 atttccaatt attgggaatt gggggaaacg gtagagattg atctgctacc ttcgcagtcc
    29161 attaccgata tattgagcga actacgccaa tcttcgccga aattacagct aaaaaccgta
    29221 ttatctcgtt atttacctaa aaaattggtt gaagtttggc tggaacagaa attgctgacc
    29281 gataaagtga ttgccgattt gagtaaaaaa gatctgacct tattagagca atttatccac
    29341 cattggcatt tcttgccaaa cggcacggaa ggctatcgta cagcggaagt aaccaaaggc
    29401 ggtgttgata ccgactatat ttcatctaaa acgatggaag cgaaaaatac agcagggctt
    29461 tattttatcg gcgaagtgct ggatgttacc ggctggcttg gtggttataa tttccaatgg
    29521 gcgtggtcat ctgcttttgc ctgtgcagaa gggattattg aaaaaagcaa ataactaaaa
    29581 aagcaaataa ctaaaaaacc aaacaaaaag gcgaacacaa ttgttcgcct tattttatgc
    29641 tgaattatct tgcacggaaa ataatgcgtg ctttgcttaa atcgtatgga gtcatttcaa
    29701 ccgttacttt atcaccggtt aaaatacgaa tgtagttttt acgcattttg cctgaaatat
    29761 gtgcggtaac aatatgaccg ttttctaatt ccacacggaa cattgtattt ggtaaggttt
    29821 ctaaaatcgt accttgcatc tcaatgcaat cttctttagc catttattcc tctttaaagt
    29881 tttaattttt atatgatgcc attcggcgaa aaacggcgga tattatacgc ttattcgctc
    29941 aaattacaat cgtttatcca tttgagtgta aactatttac gcttaatcca acggcgacgt
    30001 gagtgaaaac gctgggtttg ggtttttact tgactcataa gtggttttgg gcttatctgt
    30061 acatgatgaa ttttacgacg aaaatagtag ttaatgctac ttgccaacaa tatacctgtg
    30121 agtagcaaaa taattatttc cccataaagt tgatgaaatt gttgctctac tcgtgcttta
    30181 gtagtttgcc catattctgc atcaaacgtg acgcgtaaaa agccaaccaa cgcctgttga
    30241 gaaaatattg gctcaacaat ttgctgagtt ttggcagatt gctctgaatc gagcaatact
    30301 tttaaatctt tcgcattttg gctttgagcc agtagcacgc catttggtga atagatattg
    30361 gcatcaataa taaaatcttc tttggcaaaa ctatccaacg catcaaccag ctcatcggtt
    30421 ttggcatttt taactaacaa aaacgaaaaa atatttgctt gctggcgaac caataggtgt
    30481 gaaaggtttg atacttgatt aatgctggcc aattgtgagc ctagcttaaa ttggtttacg
    30541 cccattaaaa taaccgccac gatcccacag cagagtagcg taatccctgc cagcaaaagc
    30601 gttttcgtta ttttttcacg actaatgtac atcgaaattc cctcaaaatg atgcgtgatt
    30661 gtagcacgct tttctgttag attagtgcag taaatttaaa caacctaaaa taagacaatg
    30721 acaaacacaa tttttatcta ttcaaaaacc ttaaacgagc gtgaaattga acagttttca
    30781 caacaaacgg cagcaatttt gctgaaacaa gcggtctatt tagactacaa aattgcattt
    30841 ttttccacaa atttaaccgc ttgcgagctg cgtgaaacgg cacagaaaat caatgcggat
    30901 attagcgatc tcactattat tccaacctta aaccaaggcg gtttgctatt aatggatatg
    30961 gattccaccg ctattaaaat tgaatgtatt gatgatattg ccaaacttgc cggcaccggc
    31021 gaggtggtgt ccgccattac tgccagtgca atgcggggcg agctggattt cgagcaaagt
    31081 ttacgcaagc gagtcggcac gttggaaaat gcatcggaaa gcattttgca aaaagtgcgg
    31141 gaaaatctac cgcttatgga cggctttgag cagatggttg ctattcttaa ggaacacggc
    31201 tggaaattag cgattgcgtc cggtggattt gattacttcg ccgattactt aaaacaaaaa
    31261 tacgggttga attttgcggt ctctaaccaa ctggaaatta ttgacggtaa attaaccggc
    31321 aaggtgttag gcaaagtagt ggatgctcaa cataaagccg ataccttaca gaatttagcg
    31381 acaaccttta atattccacc aaatcaatgg gtggcagtcg gcgatggggc aaatgactta
    31441 ccaatgttaa aaaccgccag tttaggcgtt gctttgcacg ctaaaccaaa agtgcaagca
    31501 caagctgatt ttgtggtaaa cttcggggac ttaacggcat tatgtttatt acttaatgcc
    31561 aatcatttat ttcaacatta attgagagga acaacaatgc catcatttga tattgcatct
    31621 gaaatcacga tgcacgaagt gcgtaatgcg gtagaaaatg ccaatcgtgt attaagcacc
    31681 cgctacgatt tccgtggggt ggaagccgtt attgagttaa atgaaaaaaa tgaaagcatt
    31741 aagctcacga ccgaatcgga tttccaatta gagcagttaa ttgagatttt aatcggctct
    31801 tgtgtaaaac gtgggatcga gcatagttca ttagatatcc ctacagaaag tgagcatcac
    31861 ggtaaactct attccaaaga aattaagctc aagcaaggga ttgagtcaga tatggcgaag
    31921 aaaattacca agctgattaa ggattctaaa atcaaggttc aagcccaaat tcaaggcgag
    31981 caagtgcgtg taacgggcaa atctcgtgat gatttacaag ccgcaattca gctagtgaaa
    32041 ggggcagatt tagggcagcc attccaattt aataatttcc gtgattagca attcctgcaa
    32101 ttaatgatga caagtggaaa aattcagcaa aatttgaccg cttgcattaa taattcaaca
    32161 taaaaggaag agatgatgag cgagcaaaca gaaaagaaag gctggtttaa aaaagccctt
    32221 gagaaatatg atgccttttg cgatgagcta ggggtaaatc aaggtgcttg ccgaggttgt
    32281 gtccctgtgg tgaagtttga ccctgaaccg gaaaaagagc agccagaaaa atcagaaaat
    32341 aaaacataga aaaaatgcac aatggtaaga tacattgtgc attttttata ggatttttaa
    32401 ttggcgtgat gatgggcaaa atattgctta actccgccat ctttcttgca aggattctga
    32461 atatcagtag agtaactacc gttaaaacaa agagcagtag cattcgatgg tttaatcttt
    32521 tcctgtttag ttagggctgg cggttgagta ttggtttcac taatatagat cgttttaggt
    32581 tgagaatagt ctatattttt gtcgccattt acgacttgaa cgggggtgtt cgagctgcaa
    32641 gctacaaccg atgctgaagc caatgtaagt ataatgatat ggtgtttttt catgggtaat
    32701 ttccgttttt atatttttaa ttgagagaat gttaaaaagg gctgatttct caacccttaa
    32761 agttatgcct taagcatttt tattaaaagt ttgtcctaac tgaatttcat catttaatac
    32821 aggaattaac tgctctagtt gatctttttc aaattcgctg agattgccga taggtagaac
    32881 tttttccaca ccatttgtac ctaaacgaat cgggtaagca aagaatgccg gagaggtttc
    32941 gccattggca ttatctacat aagcataacg aatgacatct tccccgatta aggctctcac
    33001 taaaccaagt gcaaattctg ctcctgcttg agccatagat aaggttgctg agccaccacc
    33061 tgcttttgcc tcaaccactt cagtaccggc gttttggata cgatgagtta attgctcgat
    33121 ttgttcttga gtgaactcaa gttttaagcc atttactgtt gcttgtgaaa gcagtggaag
    33181 aatagttgtg ccggaatgtc cgccaatcac aggcactctt acatatttca catttacgtc
    33241 tttcgcttca gccacaaagg tattcgctcg aataacatct aatgttgtaa caccgaaaag
    33301 tttatttttg tcatacacac cggctttttt caatacttct gccgcaatcg gaataatggt
    33361 gtttaccggg ttagtaataa ttgcaatgca agctttaggg caaactttcg ctgctttttc
    33421 gactaagtta tgcacgatag tggcattcgt attgaataaa tcggcacgtg tcatccccgg
    33481 cttacgggca acaccagcag aaataacgac cacatcggca tcttttagtg cctcagtcgg
    33541 atcttcacct gcaaaaccgg ttacttttac atcagttgga atatggctta aatccactgc
    33601 aattcctgga gtaacagggg aaatatcgta aagagataat tcagatttag ccggtaaatt
    33661 gagttttaat aataaggcga gtgattgacc gattccacct gcagcaccta aaagggcaac
    33721 ttttttactt tgacacataa aatttctcct aaatgttatg gaagtttaga ccaatttagt
    33781 ctaagccaat tttttgggcg attcaaacgg caaatctcat ttttgagaca tagagcaaat
    33841 ttttagctga tttttatggg ttttattgat ttatttgcat aaataagcaa gaattatgaa
    33901 tatttttaag tttttgtgaa taagagtgtg aaagtggaca agatagatag tttatcagaa
    33961 gcgtttaaat cgctgttaaa ggaagaaaaa ttcggctcgc aaagtgaaat tgttgccgca
    34021 ctacaagaga tgggctttga taatatcaac caatccaaag tttctcgaat gttatcaaaa
    34081 tttggtgcag tcagaacccg taatcctaaa atggaaatgg tgtatcagct accagtcgaa
    34141 ttaagtgtac cgactacctc cagcccactt aaaaatttgg tggtagatat tgatcataat
    34201 gatgcgttaa ttgtggtcaa aaccagcccc ggtgcggctc aactgattgc acgtttgctc
    34261 gattcaatgg gtaaagccga aggtatttta ggcactattg ctggcgatga tacgattttt
    34321 ataacgccgg tacgcaatgt gcaaattgaa aaattggtga agaatgtgac acagttattt
    34381 gaaacatcat tatagaataa gtaaaggagg acttaatgtt ctccttattt tttaggagat
    34441 tattatgcac atttttataa caggtggtac gggttttatt gggcgtgaat taatccagca
    34501 attaaaatct gaaaatcacc actttacact tcttactaga gatacagaag cgaaaagtga
    34561 tgatttacag attaattatt gctattcact tgatgaactt gttgatttaa atgattttga
    34621 tgcagtaatt aaccttgccg gtgagccgat ttttgataaa cgttggacag acgaacaaaa
    34681 acagaaattg attgaaagcc gggttaacct aacgaataag ctcagtcaat taatgaatgc
    34741 aagctctaac cctccgcata cttttctctc cggttcggca atcgggattt atggcgattt
    34801 gcctccttct gcaaattttt atacagaatc gaccgcttgt tatcaagatt gctttaccaa
    34861 ccggctttgc caacagtggg aagccgctgc actaaaagcc gcaagtgata aaacccgagt
    34921 gtgtttaatt cgtaccggca cggtattttc gcccgaaggc ggagcattaa agaaaatttt
    34981 accgctttat cgatgcggat taggcggaaa acttggttca ggcaaacaat attgggcgtg
    35041 gatcagccgc cgggatcacg tacaagcggt catttttctg ctcaaaaatg caaattgttc
    35101 gggtgcttat aattttgttg caccaaatcc ggtgagaaat gaagaattta atcgtatttt
    35161 agcgaatgct gttaatcgcc cggcattctt tactgtgcct gaatttgcct tgaaattagg
    35221 cttaggcgaa cgagcttgtt tagtcttaga taatcaacag ctcattccgg aaaaactact
    35281 cgcagccagt tttgtattcc aagatccgat tttaaccgag aacgttttct taaacccata
    35341 atttaggcta taatgctgta taaaagcaca gattaaaagg tgattatgga tttctcatta
    35401 ttattagacg gtttaaatga caagcagagg gaggcggttg ctgccccgat tggtaattat
    35461 ttagtgttag ccggggcggg ttcgggcaaa actcgggttt taacccatcg cattgcgtgg
    35521 ctgattgggg tggaaaatgt gccggaatcc aatattctgg ctgttacctt taccaataaa
    35581 gcggcggcgg aaatgcgaca tcggattgag tacactcttt cccaatcggg cgataatcga
    35641 ttgttcggaa tgtgggttgg tactttccac agcattgcca accgcttact tcgttcccac
    35701 tatttagatg cggatttacc gcaagatttc caaattatgg ataccgaaga tcagcaacgt
    35761 ttactcaaac gtttactgaa actgcataat attgatgaga aacattttcc gccaaaacac
    35821 gttgcgtggt atatcaacgc ccaaaaagat aaaggcaaac gggcaaaaga tattgatcac
    35881 tataacgatc cgaatgaaaa aaaattggtg gagatttacc aaatctatca agatgcctgc
    35941 gaccgagccg gtttgttaga ttttgccgag ctgttgattc gggcttatga actattcaaa
    36001 accaaatcaa ccattttgca acgttatcaa caacgttttc agcaaatttt gattgatgag
    36061 tttcaagaca ccaacaatat tcagtacgac ttaattcgtt tgcttgccgg cgatgtcggc
    36121 aaagtgatga ttgtcggcga tgatgatcag tctatttatg gttggcgtgg ggcacaggtt
    36181 gagaatatcc aacgtttctt aaatgattat gacaacgctc aaaccattcg cttagagcaa
    36241 aattaccgct ctaccggcaa cattttaagc tgtgccaatg aattaattgc caataacgat
    36301 aaccgcttag gcaaaaattt atggacagat agcggtgatg gcgagcctgt ggatatttac
    36361 tgtgccttca acgaacttga tgaagctcgc tttgttgcct cgcaaatcaa gcaatggaaa
    36421 gaagacgagg gtgatttggc ggaatgtgcg gtgttgtatc gcagcaacag ccaatctcgt
    36481 gtgattgagg aagctttaat tcaagcgaat attccttatc gcatttacgg tggaatgcga
    36541 ttctttgaac gccaagaaat taaagatgct ctggcttatt tacgcttaat cgccaatcgt
    36601 caagacgatg cggcgtttga acgggtgatc aacacgccaa ccagaggcat tggcgatcgt
    36661 accttagaca cactccgtca aattacccgt ggtcgccaaa ttacgctgtg gcaagcaatt
    36721 caagtggcgg tgcaagaaga acaactttca gggcgtgcca cttcttcctt attgcgtttt
    36781 gtagagctga ttaatgctct tgatcaagaa accgagcaga tgtcgcttgc cgaacaaacc
    36841 gattttgtga ttaaaaaatc gggtttatat gaaatgtata agcaagaaaa aggcgaaaag
    36901 ggcgaagttc ggattgaaaa cttagaagag ttagttaccg caactaagca gtttgtgaaa
    36961 cctgacgaag cggaagagct gaccgatctt accgcatttt taacccacgc ctcacttgag
    37021 gctggagaag cccaagcctc gcaacacgaa gattatgtcg aactgatgac gctacattcc
    37081 gccaaaggat tggagtttcc ccgtgtgttt attgtgggcg tggaagaagg gattttccca
    37141 agcggaatga gctttgatga aggcaggttg caagaagaac gccgtttggc ttatgtcggc
    37201 ataacccgag ctaagaaaaa actgacgatt agctatgccg aatcaagacg gctttatggt
    37261 aaagaagaac gccacgttcc gtctcgcttt attgctgaat tacctgaaga gcatattcga
    37321 gaagtgagat tgagaggctc aattaaccga gcggcaagtt ttaaccaagc taattcattt
    37381 gcaaaaaatc cgacaaaaac gaccgcttcc tttttaaacg atgatacttg gaaaatgggg
    37441 cagaaagtga accacgctaa atttggacaa ggcacgatta ttaatgtgga gggctcaggt
    37501 gaagccaccc gtttgcaagt tgcctttgtc ggcaacggca ttaaatggct gattgccaaa
    37561 atggcaaatt tagagaggat ttaggcgaat aaaaagtttt ttgcaggaat atagatggcg
    37621 agatatattg ccccttaacc ttaaactaag ggtaaataaa at
//
LOCUS       DANLZC010000006        31714 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.006, whole genome shotgun sequence.
ACCESSION   DANLZC010000006 DANLZC010000000
VERSION     DANLZC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 31714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31714
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.006"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            401..982
                     /gene="rsxA"
                     /locus_tag="PXE44_000268"
     CDS             401..982
                     /gene="rsxA"
                     /locus_tag="PXE44_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDL5587816.1"
                     /translation="MVDYILLVISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGLA
                     TTFVLTVASLSAYLMETYLLEPLNAQFLRTLVFILVIAVIVQLTEMIVHKTSPTLYRL
                     LGIYLPLITTNCAVLGVALLNVNLANNLVESVLYGFGAAAGFSLVLVLFAALRERLAA
                     ADVPRPFQGASIALITAGLMSLAFMGFTGLVKL"
     gene            982..1599
                     /gene="rsxB"
                     /locus_tag="PXE44_000269"
     CDS             982..1599
                     /gene="rsxB"
                     /locus_tag="PXE44_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDL5587817.1"
                     /translation="MLDLPIIAYILIAIAVIALIFGAVLGYSSIKLKVESDPIVDQID
                     ALLPQSQCGQCGYPGCKPYAEAIANGDVITKCVPGGQPLVVNLAELLGVEVPPMEGMA
                     EPEMKVAFVIEDLCIGCTKCIQACPVDAIIGTNKAMHTIIPDLCTGCELCVAPCPTNC
                     IEMRPIKPTTQSWNWKFDQDLIIPIVNTTELQRKLVTGGGKNEQS"
     gene            1586..3880
                     /gene="rsxC"
                     /locus_tag="PXE44_000270"
     CDS             1586..3880
                     /gene="rsxC"
                     /locus_tag="PXE44_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDL5587818.1"
                     /translation="MSNPNLVIERIRQNKETGKLWDFPGGIHPMENKSQSNSKPIRTL
                     NLPKFFYVPLVQHSGWAGELLVKVGDVVLKGQPLTKGDHFRQLPVHSPTSGRVIGIEP
                     YVSAHASGLPEITVIIETDGKDKWVERQPIEDFLSLTSDQIIQKVYQYGIAGLGGAVF
                     PTATKLSLADKRCKLLVINGAECEPYITCDDRLMQDFTADLIEGIRILRYVLRPEEVV
                     IAIEDNKPHAIQALETALKGSNDMFVRAIPTKYPSGASDQLIQVLTGLEIPAGKRTIE
                     MGIVMHNVGTAFAVKRAIIDDEPLIERVVTLTGDKIRNKGNVWARLGTPIQHLLEQVD
                     YQADKRFPVFLGGPMMGFILPALQAPITKTANCLIAPDHFEYAPPEPERSCIRCSSCS
                     DACPVGLLPQQLYWFARAEDHDKSKEYHLDACIECGVCAYVCPSYIPLIQYFRQEKAK
                     IEEIEEKARKAEEAKIRFEAREARLQKERDARTARISQAAEKRREEVANAGGVDPVKA
                     ALERLKAKKAVETVQEIPAEVQVKPNGEPDNSDLMAQRRARRLAKQAEQAVEKSENVT
                     NALIGANNDLPLQSMNETASDKSVGANNYSPEAESEDPRKAAVAAALARAKAKKQAQQ
                     AVEKSENITNAVIGVNNDLPLQSMNETVSDKSVGANNHSPETESEDPRKAAVAAAIAR
                     AKAKKQAQQAVEKPEDIANAVIGVNNDLPLQSMNETTSDKSVGANNHSPKTESEDPRK
                     AAVAAAIARAKAKKAAQQEKSNNV"
     gene            3873..4508
                     /locus_tag="PXE44_000271"
     CDS             3873..4508
                     /locus_tag="PXE44_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDL5587819.1"
                     /translation="MFSELIPALPWSFLFIQDLIGTIVFAISGAIAARQHKMDIFGMF
                     ILAFVTGVGGGTLRDMMIGSTPVFWMKQPIYVLMITLAVVITAIFRNQISRKSWQTGL
                     LVFDAIGLGVFTVIGVQKGLDFGLHPLICVALGGVTGCFGGLIRDILRNEVPIILQKE
                     VYVTASLIGGVIFVAANSVGLESDWVYLATVFTVIIIRLLAIRFNLNLPRL"
     gene            4554..5609
                     /gene="rsxD"
                     /locus_tag="PXE44_000272"
     CDS             4554..5609
                     /gene="rsxD"
                     /locus_tag="PXE44_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDL5587820.1"
                     /translation="MFKMVSSPHTHSSNLTANFMLWVIAMMLPAFGVQLYYFGYGVLI
                     QTLLAVSLAIVIEIGIAKLRKKTTAFYLADLSGILTALILAMSIPPYAPYWLVLIGVI
                     VALLLAKHSYGGLGQNLFNPAMVGYALLLVSFPVQMTAWIVPIELLSEPPTFRDAISL
                     IFYGVTSDGFSIHQLVNSVDGIAQATPLDSMKSSLAKSGFDAVMNTPIFNGLFATGWL
                     QLNLAFLFGGLVLIYKRIIHWQIPVAMLATFAFFSGFTELFSQQSHLHFVSHFFSGAM
                     MFGAFFIATDPVTASITPRGKLIFGGLVGLLVYLIRYYGNYPDAIAFSVLLANICVPL
                     IDHYTQPRLYGTKLDNR"
     gene            5609..6241
                     /gene="rsxG"
                     /locus_tag="PXE44_000273"
     CDS             5609..6241
                     /gene="rsxG"
                     /locus_tag="PXE44_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDL5587821.1"
                     /translation="MNTSKITLNYALILGFIALLCTAVSTGVYLLTKGKIDDVTAMQQ
                     RKLLEEVVPKSHFDNDVLATCKLVQLENAPYLNRIYVAKKSENLTAYLVQGTAPDGYS
                     GDIVLLMGIEPNGNILGVRTLEHKETPGLGDKIETRVSDWILSFTNQLFSLENEADWN
                     VKKDGGKFDQFTGATITPRAVVNHIRQSAKWVVTELAQNPTMFNQFKACK"
     gene            6250..6933
                     /locus_tag="PXE44_000274"
     CDS             6250..6933
                     /locus_tag="PXE44_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDL5587822.1"
                     /translation="MEREQQIPVTNIDVAPKQSSVWKTIFADGVWKNNGALVQLLGLC
                     PLLAVSNSVTNALGLGLATMFVLICTNITVSLFRKITPHDIRIPIYVMVIATVVTAVQ
                     LLMNAFAFPVYQSLGIFIPLIVTNCIVIGRAEAYASKNEVKFSAFDGFSMGLGMTFSL
                     VVLGALREIIGNGTLFSGLDLLLGDWAKALRIDVLHIDSGLLLAILPPGAFIGLGVIL
                     AVKNIIDKR"
     gene            7374..8324
                     /gene="lpxM"
                     /locus_tag="PXE44_000275"
                     /gene_synonym="msbB"
     CDS             7374..8324
                     /gene="lpxM"
                     /locus_tag="PXE44_000275"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249087.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDL5587823.1"
                     /translation="MTRITAQDGYTHKFLWSFLHPKYWGVWLGVLGLVILAYVPVRIR
                     DKLSAFVGKMAYRYLKQKNKKGYHRAKVNLRYCFPDWSEKKREEVVEKMFITVAQTML
                     AIGETAIRPASYLQKRCEFTGFENVVKAKESGKNVIMLVPHTWSIDMAGVAMFSLGYP
                     MTSMYNPHRNALVDWLWNITRERLGGRMHTRQNGIKAFLADVRKGDVGYFLPDEDFGE
                     ELSVYADFFATEKATLPGLNKMARVANAEVIPMFSIYNAEKAKYEMEILPPLEFSGDP
                     IQSAREMNRVIEYFVEKNPEQYVWILRLLKTRRNREDIYL"
     gene            8452..8991
                     /gene="apt"
                     /locus_tag="PXE44_000276"
     CDS             8452..8991
                     /gene="apt"
                     /locus_tag="PXE44_000276"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL5587824.1"
                     /translation="MNQLDLIKSSIKSIPDYPKAGIIFRDITSLLEVPTAFQATIDAI
                     VAEFKDKGITKIVGTESRGFIFGAPVALALGVPFVLVRKPKKLPREVISQSYTLEYGE
                     DTLEIHKDAVSANDNVLVIDDLLATGGTIDATAKLIRRLGGNVEHAAFVIWLPELGGK
                     ERLEKEGVKSFSLVSFEGH"
     gene            9082..11139
                     /gene="dnaX"
                     /locus_tag="PXE44_000277"
     CDS             9082..11139
                     /gene="dnaX"
                     /locus_tag="PXE44_000277"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL5587825.1"
                     /translation="MSYQVLARKWRPQRFSEVVGQQHVLSALENSLREDRLHHAYLFS
                     GTRGVGKTSIARLFAKGLNCVNGITAEPCGECANCKAIEEGHFIDLIEIDAASRTKVE
                     DTRELLDNVQYKPTVGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFLLATTDPQ
                     KLPITILSRCMQFHLRALEQSQIARHLEFILNAENIPFEPLALEKLAKAAQGSIRDSL
                     SLTDQAIAMSNANITLAIVSQMLGLIDDNQPLELVQATAQADGEKAMAIIEQIAAKGV
                     DWQQLLSDVAEVLHKIAMLQLVKTPTTEETLVHFLAKQIPPEDVQFFYQLILNSKKEL
                     PFAPEQRAGVEMAILRALAFHPKQAEKIATVAVEKVTQPNHSVSAVTTAKPQNQISQL
                     RENFEQNQQKTVTVANTTTAVESIAQPPQAVDTAISPSAPATLSPAMAALEARKKLQA
                     QQANLAQTEKKKPELKAPQVIAKKSANQTASLQERFISLAENQHKAVLQVSKKTESAP
                     KSVEDEDYRWTWLNPELENQADSAKPSDIKQAILQERTPELVAKTIHLSCQQDKWCDI
                     ANSLNLGGFSRQIALNSYLAEQNEQHLALVLKPNMAHLDNPESRKNLEAALAEKGYSY
                     ELTIGDNSEQQTPLEIRRAIFKQLTEAAKSALLNDEKLMLLRNAFDAQIDESTIRAVA
                     ETE"
     gene            11145..12053
                     /locus_tag="PXE44_000278"
     CDS             11145..12053
                     /locus_tag="PXE44_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5587826.1"
                     /translation="MKALELIDLVKTYPTGVKAVKGINLVVEQGDFYALLGHNGAGKS
                     TTIGIISSLVNKTSGSVKVFGYDLDTQKVELKHQIGLVPQEFNFNQFEKVIDVLVQQA
                     GFYGIPRSEALVRAEKWLKLLDLWEKRTHLTRELSGGMKRRVMIARALMHNPKLLILD
                     EPTAGVDIELRRSLWDFLRDLNNQGTTIILTTHYLEEAETLCRHIGIIQHGELIENTS
                     MKALLAKLESEVFVLDLQNSEQNQPLVIQNYPHKWLDENTLEVNVKRQQGLTNLFSQI
                     SAQGAEVLSMRNKSNRLEELFINMAQ"
     gene            12055..12816
                     /locus_tag="PXE44_000279"
     CDS             12055..12816
                     /locus_tag="PXE44_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5587827.1"
                     /translation="MNLIGFYTLAYKECRRILRIWRQTLVPPVITTTLYFLIFGTLIG
                     GRIGEMNGVGYTQFIAPGLIMMTAITASYVNTASSFFLSKFTRDFEEMLISPLSTHNI
                     IWGYVTGSILRAGLAATLVMCVAMVFVSFEIYSVAIIVLTLLLTIIAFALGGVINAVF
                     ARSFDDVGLIPTFVLTPLTYLGGVFYSISLLPEFWQTVSKFNPIVYMINGLRYGFLGI
                     SDVPLGYTFAVLTSLCIGLYIIAYSLIQRGVGLRS"
     gene            12837..13592
                     /gene="modA"
                     /locus_tag="PXE44_000280"
     CDS             12837..13592
                     /gene="modA"
                     /locus_tag="PXE44_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5587828.1"
                     /translation="MKALKKLAITMALTLSGTAFSQEITVFAAASMTNVLQDIQQDFE
                     KAYPQDKITFSFASSSVLAKQIEQDAPADIFISADQKWMDYVAEKQPEKVKHRRNLVR
                     NELVLIAPTESNIEPKAIQAVDFAKELANSYLSVGDPNHVPAGKYAKKALSHYQLWES
                     VEPRLARAKNVRDALSFVERGESPLGIVYSTDAKVSNKVKVVAVFPPESYGEIVYPAA
                     AVSEKASAATFLDFLKTPKAKAKFEAAGFYPVN"
     gene            13776..14468
                     /gene="modB"
                     /locus_tag="PXE44_000281"
     CDS             13776..14468
                     /gene="modB"
                     /locus_tag="PXE44_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDL5587829.1"
                     /translation="MLNFTPQELNAIVLSLKVASIAVVLALPLAVWVAWLLARKQFWG
                     KNLLNGIMHLPLVLPPVVIGYLLLISMAKRGVIGQYLWEWFHFSLSFSWKGAVLASMV
                     MAFPLMVRSIRLAFEAVDPKLEQAARTLGAIPLKVFFTLTLPLSFSGIIAGGVLGFAR
                     SLGEFGATITFVSNIPNQTQTIPAALFTFIETPDGEMAAARLCLVAILISLIALFCSE
                     WLAERQKRYAQT"
     gene            14455..15510
                     /gene="modC"
                     /locus_tag="PXE44_000282"
     CDS             14455..15510
                     /gene="modC"
                     /locus_tag="PXE44_000282"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDL5587830.1"
                     /translation="MLKLKLLQQLGNLTLNVNLDLPNKGITAIFGRSGAGKSSLINLI
                     AGLSTAQAGFIELNGQVLFDSQNGINLPPEKRNIGYVFQEHRLFPHYSVEKNLKYGYK
                     RSDSADFLQIVELLGIAPLLNRYPASLSGGEKQRVAIGRALLSSPNILLMDEPLSALD
                     LPRKQELLDYLDKLSARLEIPILYVSHSLEEVVRLADHLVLLENGKVTAFDTLSAVWH
                     SSAFSAWQPNHQRISLLELPVEETLPHYNMLGLSLNAQRLWVNQLPRHQIGEKLRVTI
                     ASKEVSISLQKPTQSSIRNVLKGNVRKIETHLDRVDLAIQVENQQIWSTISLWSFNEL
                     AIQPEQEVYLQIKSVSL"
     gene            15565..15999
                     /locus_tag="PXE44_000283"
     CDS             15565..15999
                     /locus_tag="PXE44_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HDL5587831.1"
                     /translation="MPIVNQSALVPYSAEQMYQLVNQYEKYPEFLSGCVSTKTLSTGE
                     NELKAELHIQKLGISQTFSTHNKMTPPYKIEMALIEGPFRHLHGAWTFTPFDEQSCKI
                     ALQLNFEFSSPIVAMVFGKVFNELTLKMVNAFKQRAKEVYGV"
     gene            15989..16324
                     /locus_tag="PXE44_000284"
     CDS             15989..16324
                     /locus_tag="PXE44_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDL5587832.1"
                     /translation="MVSEQRQNPEEQKSEEQKINVEVLYAYPDKYFLKKLQVPQNATI
                     QTIILLSGVLEKYTEIDLRENKVGIFSRPAKLNETVEEGDRIEIYRPLIADPKEIRRK
                     RAAEQAEKK"
     gene            16528..17817
                     /locus_tag="PXE44_000285"
     CDS             16528..17817
                     /locus_tag="PXE44_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5587833.1"
                     /translation="MDIRQKIAHNKMTSFQWLVVGLTVLLNMLDGFDLMALAFTAKSI
                     REELQLDGAAIGSLMSAGLFGMAIGSLFLSPLADKFGRRPLLLIGTGLATLGMLLTYF
                     ATSAFEIGVWRVLTGLGVAAILTGTNVLVSEYSSQKWRGLAIAIYASGFGIGAMLGGM
                     SAVLLQGEYGWRAVFLVGSILTGAVFVLLFFVLPESVEFLISKHPVNAKARLNQIAVK
                     LGLGNGWELPEIEAKSKSKVAITRLFEPEYRRNTLLIWLAFIAISASFYFISSWTPAL
                     LEEAGMPKTQSQTVGMAISVGGTIGSLIFGFLVSRWAAKHILQAFSILAASAVVAFVF
                     AGNLGLAITLAILVGGLINGCITGLYTINPTLYAPDFRSTGVGTAIGVGRLGSIVSPT
                     LAGILLDAGWKKNELYLGAAVVLLIAAFTVHFLKQRK"
     gene            complement(17892..19346)
                     /locus_tag="PXE44_000286"
     CDS             complement(17892..19346)
                     /locus_tag="PXE44_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDL5587834.1"
                     /translation="MSEISTLAPQLLWQWFDKACAIPHPSYHENELAEFIVNWAKSKN
                     FFVERDEVGNVLIRKPATKGMENRQTVALQAHLDMVPQANTGTVHDFTKDPIQPYIDG
                     EWVKAKGTTLGADNGIGLASCLAVLDSDDLAHPTIEVLLTMTEEAGMEGAIGLRPNWL
                     TADIMINTDTEENGEIYIGCAGGENVNVTLPIFLVPHQQDSAVTVTLKGLNGGHSGCD
                     IHTTRANAIKVFARILEQAHNEVEFQIAEIQGGSVRNAIPREAHATLVFSAKNQQKLT
                     ACLSQTAEQVKAELKIAEPNFQFLIEEAQLPEQAFDLASTARVIHFLNVMPNGVVRNS
                     DVVENVVETSLSIGVLNTEASEVKATILARSLIEGGKEEIRSKIRSLTALVGAEVEFS
                     GNYPGWEPNPNSKITPLTKAIYDEILGYESQIKVIHAGLECGLINKIYPNMDFVSIGP
                     TILNAHSPDEKVHIPAVEIYWKLLTKLLEQAPEK"
     gene            19546..20765
                     /locus_tag="PXE44_000287"
                     /pseudo
     CDS             19546..20765
                     /locus_tag="PXE44_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
     gene            20961..22370
                     /locus_tag="PXE44_000288"
     CDS             20961..22370
                     /locus_tag="PXE44_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5587835.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            22516..22989
                     /gene="gpt"
                     /locus_tag="PXE44_000289"
     CDS             22516..22989
                     /gene="gpt"
                     /locus_tag="PXE44_000289"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL5587836.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            23102..23725
                     /gene="gmk"
                     /locus_tag="PXE44_000290"
     CDS             23102..23725
                     /gene="gmk"
                     /locus_tag="PXE44_000290"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL5587837.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            23815..25212
                     /locus_tag="PXE44_000291"
     CDS             23815..25212
                     /locus_tag="PXE44_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL5587838.1"
                     /translation="MKLSKLAFSVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQLAILNAKYAILT
                     NENAVYQAMAGKYRR"
     gene            complement(25325..26170)
                     /locus_tag="PXE44_000292"
     CDS             complement(25325..26170)
                     /locus_tag="PXE44_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5587839.1"
                     /translation="MTFVKKTALSLIATALFATSSFAAEQSQYQHIRNATAKIEYAGK
                     TFLVDPYLAEKDRYLPPNAKKRNPTLDMTVSIDEVLNGVDAVIVTHTHHAHWDDVAQQ
                     RVPKNLPIFVQNAGDARLIRSQGFTDVRVVGKDTPFEGVTLTRTRGQHGTDAMYANPK
                     IAEMVGDAMDVVMKAEGKPTVYLVGDTIWNHEIEHSFNTYKPDFVVLNAGCAKLPNFE
                     GSPIMCTEDVSKAHKIAPQAKILTVHMDALPQTSISSDQMRDYVQKEGLNRVTVPKNG
                     DVIKF"
     gene            complement(26311..29406)
                     /gene="ttrA"
                     /locus_tag="PXE44_000293"
     CDS             complement(26311..29406)
                     /gene="ttrA"
                     /locus_tag="PXE44_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrA"
                     /protein_id="HDL5587840.1"
                     /translation="MNNKRRNLLKGGLALGGAAAFGAGYSPKVSEIAKGVMHGTSGEK
                     TRDAIHGNSLTPEYQVKDGKLLSNPEQIVCNTQCMGCWTQCGIRARVDLNTNQVIRIS
                     GNPYHPLSSDKTLPFGMPIAKAELLLAGESGIENRSTACARGAAFLEGVNSPYRITQP
                     LKRVGKRGEGKWQTISFEQLVEEIINGGNLFGEGEVEGLKTIRNLVDPINPKQPDLGM
                     KANQLLVTFAGPEGRQPILQRFAQKSFGTINFSNHGAYCGAAYRAGSGAFMKDFDNQQ
                     HAKPDWDHAEFILFIGTSPAQSGNPFKRQARQLAKRRPDDNFSYVVVSPRLEMTSSLA
                     TQNNHWVPIKPEGDLALVMGMLRWIIENERFNADYLSRPSLAAMENAGHVSYANASHL
                     IICEPNHPKFGQALRISDLQDVQLDPEEKVEENLIVVKDQASGELISAKDCLQAVIFV
                     EQIAKLLDGSEVLVKSSMQLLKEATQEKTLAEYSDICGVPVQTIENLAKKFTSHGHKA
                     NAITHGGTMHSTGFYTSWAILLLNAMVGNLNKKGGMAVAAGKFKDFGDGPRYNLANFP
                     NEVKPKGTNLARSKKAYQNSSEFKQKVTKGENPYPAQGAWYPFTGGQTTEMFTSMVQG
                     YPYFAKAWINHMANPVYGLTAMNPIALEKLKDPKILPLFVSIDAFMNETTALTDYIVP
                     DTHNFESWGFSTAWGGVPQRTSTARHPIVPSKNAKTAEGDTICMENFVIAVAKKMGLP
                     GFGENAIADLEGNRYPLHRTEDYFLRAAANIAFAGEKPAPDASEQDLLLTGVQRIMPK
                     IEQTLKAEERLKVANVYCKGGRFAPHESAWKEENMEFQWKNCLQIWNENVYKAQHHSN
                     GEHYWGCPRYMSPRFADGSTLEQHYPQSEWGFKLISFKSNIMSSITAPLLRLLSIKPE
                     GLVAMNRLDAEEKGIKHGDVVKLSTPTAGLTVQIVVLDGIARGTIGIEHGYGHKQIGA
                     SSYTIDGVEIPANPMIAKGINLNDLGIIDHTKAVKSPWVDWVCGSAVRNGIPAKVEKL
                     A"
     gene            complement(29396..30427)
                     /gene="nrfD"
                     /locus_tag="PXE44_000294"
     CDS             complement(29396..30427)
                     /gene="nrfD"
                     /locus_tag="PXE44_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HDL5587841.1"
                     /translation="MIREVLVEPQHIVWLPWIVHYFFFVGVAATAVFTAVLFAKKSLK
                     PTACELAAVTVALIGSIVAPVALTADLHQPSRILHFYTDFAWRSPMAWGAIILPLFSV
                     AVAGYFVLALAHHTRPNLPKWLAWLQLPILQNQGLLWAFRLFATLTAIGIIGYTVLET
                     YQTGTRTLWHSAWLLPIMLFSAWAVALGLTQAVCKMLFHTQTNTGLWTKLCLILTALS
                     LVGLAFSSPQATQDFAQLFNGSITAYLVGIFWLIALICNFSAKNHRLQWLGVLALIAF
                     GWLLRWVLVIQVQTIAKTNALQNPYHFDWTAVDGGLGIVSIFGLTVLVSIVVWQLILI
                     ICGGRKGEQ"
     gene            complement(30429..31157)
                     /gene="ttrB"
                     /locus_tag="PXE44_000295"
     CDS             complement(30429..31157)
                     /gene="ttrB"
                     /locus_tag="PXE44_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrB"
                     /protein_id="HDL5587842.1"
                     /translation="MDLSKRSFLKELSALTVGASFVPLQSAQAFMPAWREGDENKRYA
                     MLIDLRKCIGCQACTVSCSVENATPLHSFRTTVRQYEITNGTQVVNNVVLPRLCNHCD
                     QPPCVPVCPVQATFQRKDGVVVINNEQCIGYGYCVQACPYDARFINEETKTADKCTFC
                     THRLEVGLLPACVESCVGGARVIGDLNDPKSQISQLYQAHKDDLKVLKPEAGTVPHVF
                     YVGLDDDAFVSKIDGQPMLWTGEA"
BASE COUNT         8770 a         6831 c         7290 g         8823 t
ORIGIN      
        1 ctttatggaa aaagatttat tatgttttca ttatatttgg gattattatg tttatttatc
       61 gagtaattaa ttggttgtta ttctaatatc ctctatatca aaggagtgag actttttagg
      121 attaacttag aaaatcaagc tcctttcacc tttcatgctg tatagcttag tccccacatt
      181 ctccattcta ccagagatag aaacaaaggt aacaagatca tatttgatat ggtatgttta
      241 ctagaagtac ctccgatttc ttattgtgaa tgagccgaaa acaagagata atataacgtt
      301 tatttttaat gagatgcttt aacaaacggt cagattttct ctgttttttg caaaataaaa
      361 tgcgtttttg accgcttgta tatttaattt tgtggaattt atggtcgatt atattctgtt
      421 ggtcatcagc accgccctta ttaataactt tgtcttagtt aaatttctag ggctttgtcc
      481 gtttatggga gtgtcgaaaa aggtggaaac tgctattggt atggggttgg cgacaacctt
      541 tgtgctgacg gttgcctcgc tctctgctta tttgatggaa acctatttgc ttgagccgtt
      601 aaacgctcaa tttttgcgta ccttggtgtt tattttagtg attgcggtga tcgtgcaatt
      661 aacggaaatg attgtgcata aaaccagccc gacactttat cgcttgcttg ggatctattt
      721 accgctgatt accaccaact gtgccgtatt aggggtggca ttattaaacg tgaatttagc
      781 caataacttg gtggaatctg ttctttatgg ttttggagcc gctgccggtt tctcgctggt
      841 gttagtttta tttgcggctc tgcgtgagcg tttggctgcc gctgatgtgc ctcgtccgtt
      901 ccaaggagca tcaattgctc tgattaccgc aggcttgatg tcgcttgcct ttatgggctt
      961 tacaggattg gtgaaactct aatgcttgat ttgccaatta tcgcttatat tcttatcgct
     1021 attgctgtga tcgcactgat tttcggggcg gttttaggct attcctcgat taaactgaaa
     1081 gttgaatccg acccgattgt cgatcaaatt gatgcccttt taccacaaag ccaatgcggt
     1141 caatgtggct acccgggctg taagccttat gctgaggcga ttgctaatgg tgatgtgatc
     1201 actaaatgtg tgccgggtgg acaaccttta gtggtgaatc ttgccgaatt gcttggggta
     1261 gaagtgccgc caatggaagg tatggcagag cctgagatga aagtggcttt tgtgattgaa
     1321 gatctctgca ttggctgtac taagtgcatt caggcttgcc cggttgatgc gattatcggc
     1381 acgaataaag caatgcacac cattattccg gatctttgca ccggctgtga actttgtgtc
     1441 gccccttgcc cgaccaactg tattgaaatg cgaccaatta aaccgacaac ccaatcttgg
     1501 aactggaaat ttgaccaaga tttaattatt ccgattgtga ataccactga attgcagaga
     1561 aaattagtta ccggcggagg caaaaatgag caatcctaat ttagtgattg agcgaatccg
     1621 ccaaaataaa gaaacgggta aactgtggga tttcccgggc ggtattcacc cgatggaaaa
     1681 taaatcccaa tcaaacagca agccaattcg caccttaaat ttaccgaagt ttttctatgt
     1741 gccactggtt caacattctg gctgggcagg ggaattatta gtcaaagtcg gcgatgtggt
     1801 attgaaaggg cagccgctca cgaaaggcga tcacttccgt caactgcccg tacacagccc
     1861 aacctccggg cgggtgattg ggattgagcc ttatgtttcc gctcacgcct caggtttgcc
     1921 cgaaataacg gtgattattg aaaccgatgg taaagataaa tgggtggaac gccagccgat
     1981 tgaggatttt ttaagcctaa caagcgatca aattatccaa aaagtttacc aatatggcat
     2041 tgccggttta ggcggtgcgg tattcccaac cgcaacaaaa ttaagtttgg cggataaacg
     2101 ctgtaaatta ctggtgatta acggggcaga atgtgagcct tacatcacct gtgacgatcg
     2161 cttaatgcaa gatttcactg cggatcttat cgaaggtatt cgcattctgc gttatgtgct
     2221 tcgcccggaa gaagtggtga ttgcgattga agacaacaaa ccgcacgcta ttcaagcttt
     2281 agaaaccgct ttaaaaggct cgaacgatat gtttgtgcgg gcgatcccga ctaaatatcc
     2341 ctccggagct tctgatcagc tgattcaagt gttgaccgga cttgagattc cggctggcaa
     2401 acgcactatt gaaatgggca ttgtgatgca caatgtcggc acggcgtttg cggttaaacg
     2461 ggcaattatt gatgatgaac ctttgattga acgggtagtg acgctgacag gcgataaaat
     2521 tcgcaacaag gggaacgtgt gggcaagatt aggcacgcca attcagcact tgttggagca
     2581 ggtagattat caagccgata aacgctttcc ggtgttttta ggcggaccga tgatgggctt
     2641 tattttgcct gcattgcaag ccccgattac caaaactgcg aactgcttga ttgcccctga
     2701 tcattttgaa tatgcaccac ctgaaccgga gcgtagttgt atccgctgtt caagctgttc
     2761 cgatgcttgc ccggtggggt tattgccaca gcaactttat tggtttgccc gtgcagaaga
     2821 tcacgataaa tcgaaagaat accatttaga tgcctgcatt gagtgtggcg tttgtgctta
     2881 cgtttgccca agctacattc cgttaatcca atatttccgc caagaaaaag caaaaatcga
     2941 ggaaattgag gaaaaagccc gcaaagcgga agaggctaaa atccgctttg aggcgagaga
     3001 agctcgctta caaaaagaac gagatgccag aactgctcgc attagccaag cggcggaaaa
     3061 acgccgtgaa gaagtggcaa atgcaggtgg tgttgatcct gtaaaagcgg ctcttgaacg
     3121 cctgaaagcg aaaaaagcgg ttgaaacagt ccaagaaata ccggcagaag tacaggtaaa
     3181 acctaacggc gagcctgata acagcgattt aatggcacaa cgcagagcca gacgtttagc
     3241 caaacaggca gaacaagcgg tcgaaaaatc ggaaaatgtt acaaatgcct tgattggggc
     3301 aaataatgat ttgcctctac aatcaatgaa cgaaactgca tcggataaat ctgtaggggc
     3361 gaataactat tcgcccgaag cagaaagcga agacccacgc aaagccgccg ttgcggctgc
     3421 ccttgcccga gccaaagcca aaaaacaggc acagcaagcg gtcgaaaaat cagaaaatat
     3481 taccaatgcc gtgattgggg taaataatga tttgccccta caatcaatga acgaaacggt
     3541 atcggataaa tctgtagggg cgaataacca ttcgcccgaa acagaaagcg aagacccacg
     3601 caaagccgcc gttgcagcag ctatcgctag agctaaagcg aaaaaacaag ctcaacaagc
     3661 ggtcgaaaaa ccggaagata ttgccaatgc tgtgattggg gtaaataatg atttgcccct
     3721 acaatcaatg aacgaaacta catcggataa atctgtaggg gcgaataacc attcgcccaa
     3781 aacggaaagc gaagacccac gcaaagccgc cgttgcggcc gccatcgcca gagctaaagc
     3841 gaaaaaagcc gctcagcagg agaaaagcaa caatgtttag cgagctaatt ccggcattgc
     3901 cgtggtcgtt cctgtttatt caggacttaa tcggcactat cgtgtttgcg atttcggggg
     3961 caattgctgc ccgccagcac aaaatggaca ttttcggtat gtttatttta gcgtttgtga
     4021 ccggggtcgg gggcggtacg ctaagggata tgatgattgg ctccacgccg gtgttttgga
     4081 tgaaacaacc gatctatgtg ctgatgatta cccttgcagt ggtgattacc gctattttcc
     4141 gcaaccaaat ttcccgcaaa agttggcaaa ccggcttgct ggtgtttgat gccatcgggc
     4201 ttggcgtatt taccgtgatt ggcgtgcaaa aaggcttgga tttcggcttg cacccactga
     4261 tctgtgtggc tctgggcggt gtgacaggct gttttggtgg cttaatccgt gatattttac
     4321 gcaacgaagt gccgatcatt ttgcaaaaag aagtgtatgt gaccgccagc cttatcggtg
     4381 gtgtgatttt tgtggctgca aactcagttg ggctcgaaag cgattgggtt taccttgcca
     4441 ccgtgttcac cgtaattatt atccgcctgc tggcaatacg ctttaatttg aatttgccga
     4501 gactttaata agcggtcatt tttttgcaat tttttactaa aaaggtttta tagatgttta
     4561 aaatggtcag ttccccccac actcattcga gcaatctcac tgcgaatttt atgctgtggg
     4621 tgattgcgat gatgttacct gcttttgggg ttcagcttta ttatttcggc tatggtgtgc
     4681 tgattcaaac gttgcttgcc gttagccttg cgattgtgat tgaaatcggg attgcaaaat
     4741 tacgcaaaaa aacaaccgct ttctatttgg ctgacttatc aggtatttta accgccttga
     4801 ttttggcgat gtcgattccg ccttatgctc cttattggct tgtgttgatt ggcgtgattg
     4861 tggcgttatt attggcaaaa cacagttatg gtggcttggg gcagaatcta tttaacccgg
     4921 caatggttgg ctatgcgttg ttgttggttt ctttcccggt gcaaatgacg gcgtggatag
     4981 tgccgattga gttattaagt gaaccaccga catttcgtga tgcgatttcg ttgatttttt
     5041 acggggtaac tagcgatggt tttagtattc atcagctagt taatagtgtt gatggcattg
     5101 cacaagctac cccgcttgat agtatgaaaa gcagccttgc aaaatcaggt tttgacgcgg
     5161 taatgaatac gccgattttc aacgggctgt ttgcgaccgg ttggctacaa ctgaaccttg
     5221 cgttcttatt cggcggtttg gtgttgattt ataaacggat tatccattgg caaattccgg
     5281 tagcaatgtt ggcaactttt gcctttttta gtggattcac cgagctgttt agccaacaat
     5341 cgcacttgca ttttgtgagc catttcttta gcggtgcgat gatgttcggg gcatttttta
     5401 ttgcgaccga cccggtaaca gcctccatta ccccaagagg aaaactgatt ttcggtggct
     5461 tggtgggatt attggtttat ctaatccgct actacggaaa ttaccctgat gcgattgcat
     5521 tttcagtgtt attggcaaac atttgcgtac cgttaatcga ccattatacg cagccgagat
     5581 tatacggcac aaaattggat aacagataat gaacacctca aaaattacct taaattacgc
     5641 cttaatttta ggctttattg ccttgctttg caccgctgtt tcaaccggtg tgtacctgct
     5701 taccaaaggc aagattgatg atgtaaccgc aatgcaacaa cgcaaattat tggaagaggt
     5761 tgtgccgaaa agccattttg ataatgatgt gttggcgact tgcaaactgg ttcagctcga
     5821 aaatgcccct tatttgaacc gtatttatgt tgcaaaaaaa tcggaaaatc tgaccgctta
     5881 cctagttcaa ggaacggctc cggacggcta ttccggcgat attgtgctat tgatggggat
     5941 tgagccgaac ggcaacattt taggcgttcg cacccttgag cataaagaaa ctccggggct
     6001 gggagataaa attgagaccc gagtgtcgga ttggatctta tcttttacca atcaactttt
     6061 tagtttagaa aacgaagcgg attggaatgt aaaaaaagat ggcggcaagt tcgaccaatt
     6121 taccggtgca actattacac ctcgtgcggt ggtcaatcat atccgccaaa gtgcgaaatg
     6181 ggtggtgaca gagctggctc aaaacccgac aatgtttaat caatttaaag cgtgcaaata
     6241 gggggagaaa tggaaagaga acaacaaatc cctgtgacga atattgacgt tgccccgaaa
     6301 caatccagcg tttggaaaac gattttcgct gatggtgtgt ggaaaaataa tggtgctttg
     6361 gtgcaattat tagggctttg cccactactg gcggtttcaa acagcgtaac caatgcactt
     6421 ggactaggct tggcaacaat gtttgtgcta atttgcacca atatcacggt ttccctgttt
     6481 cggaaaatca ctccacacga tattcggatt ccgatttatg tgatggtaat tgcaacggtg
     6541 gtaacggcag tacagctttt aatgaatgct tttgctttcc ctgtttacca atcgctcggg
     6601 atttttattc cgctgattgt aaccaactgt atcgtgattg gacgagcaga agcctatgcc
     6661 tcaaaaaatg aggtcaaatt ctcggctttt gacggctttt caatggggct gggaatgact
     6721 tttagccttg tggtgctagg cgcgttgcgt gaaattattg gcaacggcac gctgttttca
     6781 ggcttagatc tactccttgg cgattgggca aaagccctac gcattgatgt attgcatatt
     6841 gattcaggtt tactactggc aattttacct ccgggggctt ttattggact tggggtgata
     6901 ttggcggtga agaatattat tgataagaga tgatgtgtta tgtcactttt ctttgcttgt
     6961 gcaaagaaaa gtaaccaaaa gaaagcacac cctgctttgt tgctatcctc acttggttga
     7021 aattttctta acgaaaaata accctgatgt tcgcttcgct ctcgctcgag ttattttcct
     7081 aaaaatttca agtcgttcgg gcaactcaga agggaacccg atccgaccat acaagcggtg
     7141 atttttttgg aaaattttgc aaaatgatgc tctgccgggt tcccttctga accgcccgaa
     7201 cgactttgaa tttgtaggaa aaattccttg tttgagcagc gaagcggcga gtttggaatt
     7261 ttttcgttaa gcaaattcaa accaagcgaa gatagcggtg aagtagggtt gcctttcttt
     7321 ggttactttc tttggcgaag caaagaaagt gacaaataat gaggaattat agaatgacac
     7381 gaattaccgc ccaagacggc tatacgcaca aattcctatg gtcttttcta cacccgaaat
     7441 attggggcgt atggttaggt gtattaggat tagtgatttt agcctatgtg ccggtgcgaa
     7501 ttagggataa attatccgct tttgtgggta agatggcgta tcgctatctc aagcagaaaa
     7561 ataaaaaagg ctaccaccgt gctaaagtga atttgcgtta ctgttttccc gattggagtg
     7621 agaaaaagcg tgaagaagtg gtcgagaaaa tgtttatcac ggtagcacaa accatgttgg
     7681 cgattggcga aactgcaatc cgcccggcct cttatttaca aaaacggtgt gaatttaccg
     7741 gctttgaaaa tgtggtgaaa gccaaagaga gcggtaaaaa cgtgattatg ttagtgccac
     7801 atacttggtc gattgatatg gcgggggtgg cgatgttctc gctcggttat ccgatgacat
     7861 caatgtacaa tccacaccgt aatgcgttgg tggactggct gtggaatatt acccgtgagc
     7921 gtttaggtgg gcgaatgcac actcgtcaaa atgggattaa agcgttttta gccgatgtgc
     7981 gtaaaggtga tgttggttat tttctgcccg atgaagattt cggcgaagag ctaagtgtgt
     8041 atgccgactt ttttgccacc gaaaaagcca ctttgccggg cttaaataaa atggcaagag
     8101 tggcaaatgc ggaagtgatc ccaatgttct cgatttataa tgcggaaaaa gcaaaatatg
     8161 aaatggaaat tttgccaccg cttgaatttt caggagaccc aattcaatcc gcccgagaaa
     8221 tgaaccgagt aattgaatat tttgtggaga aaaaccccga gcagtatgtt tggattttac
     8281 gtttgcttaa aaccagacga aacagggaag atatttatct ataatatagg gaaaaggaaa
     8341 ctgaaaagta agctgtacta tcaaacgtaa ggttatcaat ttctccttga aatttctgct
     8401 gtttttctat agagttttcc agttattttt ttattacacg taggtcaaac aatgaaccaa
     8461 ttagatttaa ttaaatcttc tatcaaatcc attccagatt atccgaaagc agggattatc
     8521 ttccgtgata tcacttcact tttagaagtg ccaactgcat tccaagccac cattgatgcc
     8581 atcgttgcag aatttaaaga caaaggtatt actaaaattg tcggcacaga atctcgcggg
     8641 tttatttttg gtgcccctgt tgcattagcg ttaggcgtgc cttttgtgtt ggttcgtaaa
     8701 ccgaaaaaat taccacgtga agtgatttcg caatcttaca cgcttgaata tggtgaagac
     8761 acgctagaaa ttcataaaga cgcagtgagt gctaatgata atgtattggt catcgacgat
     8821 ttactcgcaa ccggtggtac tattgatgca actgcaaaac taattcgtcg cttaggtggt
     8881 aacgtagaac acgctgcctt tgtgatttgg ctgcctgagt taggtggtaa agagcgtttg
     8941 gaaaaagaag gggttaaatc ctttagccta gtcagctttg aaggtcatta atggggaaca
     9001 agcggtggct ttttgctaaa attttgcaaa atttttcgga aagatgaccg cttgtgattt
     9061 ccaaattgaa tagaaaaatc aatgagttat caagttttag cccgtaaatg gagaccgcaa
     9121 cgcttctcgg aagtagttgg tcagcaacac gttttatctg ctcttgaaaa cagcttaaga
     9181 gaagatagac tgcaccacgc ttacctgttt tcaggcactc gtggtgtggg taaaacctct
     9241 attgcccgtt tattcgccaa aggcttaaac tgtgtaaacg ggatcaccgc cgagccttgc
     9301 ggtgaatgtg cgaattgtaa agcgattgaa gaagggcatt ttatcgatct cattgaaatt
     9361 gatgctgctt ctcgtactaa agtggaagac acccgtgagc tacttgataa cgttcaatac
     9421 aaacccaccg ttgggcgttt taaggtttat ttaattgacg aagtgcatat gctctctcgt
     9481 catagtttca acgccttact aaaaaccctt gaagagccgc ctgaatatgt caaattcttg
     9541 ctggctacca ctgatccgca aaaactgccg attacgatcc tctctcgctg tatgcaattt
     9601 catctgcggg cattagagca atcgcaaatt gcccgtcatc ttgaatttat tctaaatgca
     9661 gagaacattc cgtttgagcc tttggcacta gaaaagttgg caaaagcagc acaaggtagt
     9721 atccgagact ctctcagcct caccgatcaa gccattgcaa tgagtaatgc caatattacg
     9781 cttgcgatag tcagccaaat gctagggttg attgacgata atcaaccact agaattagtg
     9841 caagcaaccg cccaagccga cggtgaaaaa gcaatggcga tcatcgagca aattgcagcg
     9901 aaaggcgttg attggcaaca attattaagc gatgtggcag aagtgttaca caaaattgca
     9961 atgttgcaat tagtgaaaac acctacaacc gaagaaacat tagtgcattt tctggcaaaa
    10021 caaattccgc ccgaagacgt gcaatttttc tatcaattaa tcctcaacag taaaaaagag
    10081 ttgccttttg caccggagca acgtgccggt gttgaaatgg cgattttgcg tgcgttggct
    10141 ttccacccta aacaggcaga aaaaatagca acagttgcag tggaaaaggt tacacaacca
    10201 aaccattcgg tttcagcggt tacgacagca aaaccacaaa atcaaattag ccagctaaga
    10261 gaaaattttg agcaaaatca gcaaaaaacg gttactgttg ccaatactac cacagcggtt
    10321 gaaagcattg ctcaaccgcc acaggcagta gataccgcta tttcgccgtc tgctcctgcg
    10381 actctttcac cggcaatggc agccctcgaa gcccgcaaaa agctacaagc acagcaggca
    10441 aatttagctc aaaccgaaaa aaaaaagcct gagctaaaag cccctcaagt tattgcaaaa
    10501 aaatcggcaa atcagaccgc ttcattgcaa gagcggttta tcagcttggc ggaaaatcag
    10561 cacaaagccg tactgcaagt ttcaaagaaa accgaatccg cccctaaaag tgtggaagat
    10621 gaagattatc gctggacgtg gctcaatcct gaattagaaa atcaagcaga cagtgccaaa
    10681 ccttctgata ttaaacaagc gattttgcaa gaacgcaccc ctgaattagt ggcaaaaacc
    10741 attcatttat cttgtcagca ggataaatgg tgtgacatcg ccaactcact caatttaggc
    10801 ggattttccc gtcagatcgc cttaaatagc tatttagcgg aacaaaatga acagcacctc
    10861 gcattggtgt taaagccgaa tatggctcac ttagataatc cggaatcacg taaaaattta
    10921 gaagctgccc ttgccgaaaa aggctattca tacgaattga ccattggtga caatagcgaa
    10981 cagcaaactc cgcttgaaat tcggcgagcc attttcaaac aattaactga ggcagcaaaa
    11041 tcagctctgc ttaacgatga aaaattaatg ttacttcgca acgcttttga tgcacaaatt
    11101 gatgaatcga ccattcgagc ggtggcggaa actgagtaaa acaaatgaaa gcattagaat
    11161 taattgattt ggtaaaaacc tacccaacag gtgtaaaagc cgttaaaggc attaatcttg
    11221 tggtcgagca aggtgatttc tatgccctac ttgggcataa cggggcaggt aaatctacta
    11281 ctattggcat tatcagctca ctggtaaata aaaccagcgg cagcgtgaaa gtgtttggct
    11341 atgatttaga cacccaaaaa gtcgaattaa agcatcaaat cggcttagtg ccacaagagt
    11401 ttaatttcaa tcagtttgaa aaagtgattg atgtgctagt gcaacaagcc gggttttacg
    11461 gcattcctcg cagtgaagcc ttagtacgtg ccgaaaagtg gctgaaattg ctcgacctat
    11521 gggaaaaacg cacccacctc acccgtgagc tttctggtgg aatgaaacgt cgggtgatga
    11581 ttgcccgagc cttaatgcac aatccaaaac tactgatttt agatgaacct accgcagggg
    11641 tggatattga actccgccgc tcattgtggg atttcttgcg ggatctaaac aatcaaggca
    11701 caactattat tctcactacc cactatttag aagaggcgga aaccctatgc cgtcacatcg
    11761 gcattattca acacggcgaa ttaattgaaa acacctcaat gaaagccttg cttgccaaat
    11821 tggaaagtga ggtatttgtg ttagatttgc aaaattctga gcaaaatcaa ccgcttgtga
    11881 ttcaaaatta cccgcataaa tggcttgatg aaaacaccct tgaagtgaat gtaaaacgcc
    11941 agcaagggct aaccaatctg ttttcgcaaa tttccgccca aggggctgag gtgctaagta
    12001 tgcgaaacaa atcaaaccgc ttggaagaac tatttatcaa tatggctcag taaaatgaat
    12061 ttaatcggat tttatacctt agcttacaaa gagtgccgcc gcattttgcg gatttggcga
    12121 caaactttag tgccaccggt gattactacc acgctctatt ttttaatctt cggcacgctg
    12181 attggtgggc gaatcgggga aatgaatggc gtgggttaca cccaatttat tgcacccggc
    12241 ttaattatga tgaccgccat caccgcctct tatgtgaata ccgcctcttc atttttcttg
    12301 agtaaattta cccgagattt tgaagagatg ctgatttcgc cactctctac ccataatatt
    12361 atttggggtt atgtcaccgg cagtattttg cgtgcaggtt tagccgcaac cttagtaatg
    12421 tgcgtggcaa tggtgtttgt ctcgtttgaa atctactcgg ttgcgattat cgtcttaacc
    12481 ctgttgctga caatcattgc ctttgcgtta ggtggcgtaa ttaatgcggt gttcgcccgc
    12541 tcctttgatg atgtggggct gattccaacc tttgtgctta ctcccttaac ttacttaggc
    12601 ggcgtatttt actctatttc attactgcct gaattttggc aaacggtgtc taaattcaac
    12661 ccgatcgttt atatgattaa cggcttaaga tatggctttc tcggtatcag cgatgtgccg
    12721 ttaggttata cttttgcagt gcttacttca ctttgcatcg gcttgtatat tattgcctat
    12781 tccttaattc aacgaggggt tgggctgcgt tcttaactaa ttgtgaggtt atgaaaatga
    12841 aagcattaaa aaaactagca attacaatgg cattaacgtt atcaggcaca gccttttcac
    12901 aagagattac cgtgtttgct gccgcttcaa tgacaaatgt gttacaagat attcagcagg
    12961 attttgaaaa agcctatcca caggataaaa tcacgttctc cttcgcttca tcatcggtat
    13021 tagcaaagca gattgagcaa gatgcaccgg cagatatttt tatttccgcc gaccaaaagt
    13081 ggatggatta tgttgccgaa aaacaacctg agaaagttaa acatcgccgc aatttagtca
    13141 gaaacgagtt agtattaatt gcacctaccg aaagtaacat tgagccaaaa gcgatacaag
    13201 cggtcgattt tgccaaagaa ttagcaaaca gctatttatc ggtgggagac ccaaaccacg
    13261 ttcctgccgg caaatatgcc aaaaaagccc ttagtcatta ccaattatgg gaaagtgttg
    13321 aacctcgttt agcgagggca aaaaatgtac gagatgccct ctcttttgtc gagcgaggcg
    13381 aatccccact tgggattgtc tattccaccg atgctaaagt gtcgaataaa gtcaaagtgg
    13441 ttgccgtttt cccaccggaa agctacggcg aaattgttta ccctgctgcg gcagtcagtg
    13501 aaaaagcctc tgccgccaca tttttagatt ttctcaaaac cccgaaagca aaagcgaaat
    13561 ttgaggcagc tgggttttat cctgtaaatt aaatttactc tctccccttg tgggagagag
    13621 acagaaaaat atgtcgttca gacatatttt tcagagagag ggcaaatagc gtttaaaata
    13681 ttccccctct ccctgatttt tcttctgaac gaagaaaaat ctatccctct cccacaaggg
    13741 gcgagggtaa gcttataacc aaatagtttt agattatgct taatttcacc ccacaagaac
    13801 tcaatgcgat tgttctaagc ctaaaagttg cctccattgc tgtagtgctt gcattaccgc
    13861 ttgccgtgtg ggtggcgtgg ctgcttgccc gcaaacagtt ttggggaaaa aatctgctca
    13921 atggtattat gcatctccct ctggtgttac cgccggttgt cattggctat ttattgctta
    13981 tttcgatggc gaaacgaggg gtaatcgggc aatatttgtg ggaatggttt catttcagct
    14041 tgagtttttc gtggaaagga gcagtgcttg cctcaatggt gatggcgttt ccgctaatgg
    14101 tgcgttctat ccgtttagcc tttgaggcag ttgatcctaa attggagcaa gccgccagaa
    14161 cgcttggagc aataccgtta aaagtgtttt tcacgctcac tttaccgctc tctttttcag
    14221 gcattattgc cggtggcgtg ctgggttttg cccgctcttt gggggagttt ggggcaacca
    14281 tcacctttgt ctccaacatt cccaaccaaa cccaaaccat tcctgccgcc cttttcacct
    14341 ttattgaaac ccccgatggc gaaatggctg ccgcccgttt gtgcttggtg gcgattctga
    14401 tttcactcat tgccctgttc tgttcggaat ggctggcaga aaggcaaaaa cgctatgctc
    14461 aaacttaaac ttcttcagca actcggcaat ctgacgctca atgtcaatct cgatttgccg
    14521 aataaaggaa tcaccgctat tttcgggcgt tccggtgccg gtaaatccag cctgattaat
    14581 ttaattgcgg ggctttcaac cgctcaagcc ggctttatcg aattaaacgg tcaggtgctg
    14641 tttgacagcc aaaacggcat caatttacca cctgaaaaac gcaacattgg ctatgtgttt
    14701 caagagcatc gccttttccc tcattattcg gtggaaaaaa acttaaaata tggctacaag
    14761 cggtcggatt ctgcggattt tttgcaaatt gtggaactgt taggcattgc ccctttgctt
    14821 aatcgctatc cggcaagcct ttccggtggc gaaaaacaac gtgtggcgat tgggcgggcg
    14881 ttattaagct caccgaatat tttgctgatg gacgaaccat tatctgccct cgatctccct
    14941 cgtaagcaag agctactgga ctatttagat aaattatccg ctcggcttga aataccgatt
    15001 ctgtatgtca gccacagttt agaggaagta gttcgattgg cagatcatct tgtgctgttg
    15061 gaaaacggca aagtaaccgc atttgatacc ctttcagcgg tttggcacag ctcggcattt
    15121 tccgcttggc agccaaatca tcagcgaatt agcctgcttg aattaccggt ggaagaaaca
    15181 cttccccact ataatatgct tggcttgagc ttgaacgcac agcgattatg ggtgaaccaa
    15241 ctcccccgcc atcaaatagg cgagaagcta agggtaacta ttgccagcaa agaggtttca
    15301 atcagcttac aaaaaccgac tcaaagctca attcgtaatg tgttaaaagg caatgttcgg
    15361 aaaatagaaa cgcatttaga tcgggttgat ctggcaattc aagtcgaaaa tcagcaaatt
    15421 tggagtacga tcagcctttg gtcttttaat gaattagcga ttcaaccgga gcaagaggtc
    15481 tatcttcaaa tcaaaagcgt ttctttatag gcttgcaaat ttacgctaaa atatgaccgc
    15541 ttgttaattc ccaaaagaga cattatgcca atcgttaatc aatctgccct cgtgccttat
    15601 agtgccgagc aaatgtatca acttgttaat cagtatgaaa aataccccga atttttatcc
    15661 ggctgcgtga gtaccaaaac ccttagcacc ggcgaaaatg aattaaaggc agagttacac
    15721 attcagaaat taggcattag ccaaactttc agtacccaca ataaaatgac gccgccctat
    15781 aaaatcgaga tggcattaat tgaagggcct ttccgccatt tacacggagc ttggacattc
    15841 accccctttg atgagcaaag ttgcaaaatt gccctgcaat taaattttga attttccagc
    15901 ccaattgtag caatggtgtt cggcaaggtg ttcaacgaat taactttaaa aatggtaaat
    15961 gctttcaaac aacgtgcaaa agaggtgtat ggtgtctgaa caacgtcaaa atccagaaga
    16021 acaaaaaagt gaagaacaga aaattaatgt tgaggtgctt tatgcttacc ctgataaata
    16081 ttttctgaaa aaactgcaag taccgcaaaa cgcgaccatt caaactatta ttttgctctc
    16141 cggcgtgttg gaaaaatata ccgaaattga cttgcgtgaa aacaaagtcg gtatttttag
    16201 ccgcccggca aaactgaatg aaacggtgga agaaggcgac agaattgaga tctatcgccc
    16261 gttaattgct gatccgaaag aaatcagacg caaaagagcg gctgagcaag cggagaagaa
    16321 atagctttct cctattcaat ctaggggtga ataattattc gccctatcta ttttctccct
    16381 cgccattcct gctataaatt ccctcctttg attaaatatg aaaatctttc ataaatttta
    16441 tgtaaatttt gagtttgatt atatttaatc aactttctat agttcactct aaataaaatt
    16501 ttatcaattt tgatgcagag ggagcttatg gatattcgtc aaaaaattgc tcataacaaa
    16561 atgacttcat tccaatggtt agtggttggg cttacggtgt tacttaatat gctggacggc
    16621 tttgatttaa tggcactcgc ctttaccgcc aaaagcatcc gagaagaatt gcagttagac
    16681 ggtgcagcca tcggcagctt aatgagtgcc ggtttattcg gtatggcgat aggctcactc
    16741 tttctttcac cacttgctga taaatttggt cgccgcccgt tgcttttaat cggcacaggc
    16801 ttagccacgt taggaatgct cctaacttat ttcgctactt ctgcgtttga aattggtgta
    16861 tggcgtgttt taaccggttt aggcgtagcc gccattttaa ccggcactaa tgtgctagtg
    16921 agtgaatact ccagccaaaa atggcgtggc ttagcgattg caatttatgc ctcaggcttt
    16981 gggattggtg caatgctggg cggaatgtct gcggtgttat tgcaaggaga atatggctgg
    17041 cgtgcggttt tcctcgttgg gtcgatttta accggtgcgg tattcgtttt attattcttt
    17101 gtattgcccg aatcggtgga atttttaatt tcaaaacacc cggtgaatgc taaagctcgt
    17161 ttaaatcaaa ttgccgttaa attaggctta ggaaacggct gggaacttcc tgaaattgag
    17221 gcaaaaagta aaagcaaagt ggcaattacc cgtttattcg agcctgaata tcgccgcaat
    17281 acgttgctaa tttggcttgc ctttattgca atttcagcct cattttactt tatcagctct
    17341 tggactccgg cactattaga agaagccgga atgcctaaaa cccaaagcca aactgtggga
    17401 atggcaattt cagttggtgg tacaatcggc tcgttaattt tcggcttttt agtcagtcgc
    17461 tgggcggcaa aacatatttt gcaggcattc agtattctgg ctgcatctgc tgtggttgcc
    17521 tttgtgtttg ccggcaattt agggcttgcg ataacactag ccattttggt tggcggatta
    17581 attaacggct gcattaccgg gctttatacc attaacccaa ccctatacgc accggatttc
    17641 cgcagcacgg gcgtaggcac cgccatcggt gtagggcgtt taggctcaat tgtttcccca
    17701 actcttgcag gtattttatt agacgccggc tggaaaaaga acgagcttta tttaggtgca
    17761 gcagtcgtct tattaattgc tgctttcacg gtacattttt taaaacagag aaaataagag
    17821 ctaccccgta attaattgag gttttattca attaattacg gggcaagatt atataatttt
    17881 tttgattaaa tttatttctc aggtgcttgt tcaagtagtt tggttaggag tttccaataa
    17941 atctccactg ccggaatatg cactttttcg tccggagaat gggcgttgag aatagttggt
    18001 ccaatcgaga caaaatccat attcgggtag attttgttga ttaagccaca ttctagccct
    18061 gcgtgaatca ctttaatttg agattcataa cctaaaattt catcataaat cgctttggtt
    18121 aatggcgtga ttttggaatt tgggtttggc tcccagcccg ggtagttgcc cgaaaattcc
    18181 acttctgctc ccactaatgc ggttaatgag cggattttac tgcgaatttc ctcttttccg
    18241 ccctcaatta atgagcgggc aagaatggtc gctttcacct cactcgcttc ggtgttaagc
    18301 acaccaatac tgagtgaggt ttccaccaca ttttccacca catcgctatt acgcaccaca
    18361 ccatttggca tcacatttaa gaaatggatt actcgagcag tgctggcaag gtcaaaggct
    18421 tgctcaggca gttgagcttc ttcaattaaa aattggaagt ttggctcggc aattttgagt
    18481 tctgctttta cttgttcggc ggtttggctt aaacaagcgg ttaatttttg ctgatttttt
    18541 gcagagaaaa ccaaggttgc gtgggcttct cgtggaatgg cattccgcac cgaaccgcct
    18601 tgaatttctg caatttggaa ttccacttcg ttgtgggctt gttccaaaat gcgggcgaac
    18661 actttaatcg cattcgctcg ggtggtgtga atatcacagc ccgaatgccc gccgtttagg
    18721 cctttaagcg taacggttac tgcactatct tgctgatgag gcaccaagaa aattggcagt
    18781 gtcacattca cattttcccc acctgcacag ccgatgtaaa tttccccgtt ttcttcggtg
    18841 tcggtgttaa tcataatatc ggcggttagc cagttcgggc gtaaaccaat cgccccttcc
    18901 ataccggctt cttcggtcat tgtgagcaac acttcaatgg ttgggtgggc taaatcatcg
    18961 ctatccaaca ccgccaagca cgaagccaat ccaatgccgt tatctgcccc caatgttgtg
    19021 cctttggctt ttacccattc gccgtcaata taaggctgaa tcggatcttt agtgaaatcg
    19081 tgaaccgtgc cggtattggc ttgtggcacc atatcaagat gggcttgcag agcgacagtt
    19141 tggcggtttt ccataccttt agttgccggt ttacggatta acacattacc cacttcatca
    19201 cgttcaacaa agaaattttt cgattttgcc caattcacaa taaattcagc cagttcattt
    19261 tcgtggtaag acgggtgcgg aatagcacag gctttatcaa accactgcca aagtagctgc
    19321 ggtgcgagcg ttgagatttc agacataata agcccctttt gtaaaatttt aagaaaaaat
    19381 aaccgcttgt attcaagctg atatagacct ctgacattgt agaatattta ggagaaattt
    19441 aacaaaaaaa tcttaaaaag tacttgatga cgagttgttt ttttgtacaa tatgcgtgtg
    19501 ttttatacac atctattttt gtttatctta caaaaggaac attttatgtc tatcaaaaaa
    19561 gtaactttag ccagtttgat tttattgggt ttaacggcgt gtagtagtgg tggttcgggc
    19621 agtagctcaa acaatgtggc aaaaccgaat actaaaccgc aaactactca gcaacaaacc
    19681 gataaagcgg cagcagaaaa agcagcaaaa gacaaggcag cgaaagagaa agcagaagca
    19741 gaacgtttag cagcagaaaa agcagctaaa gagaaagcgg aagcagaacg tttagcggcg
    19801 gaaaaagcag cgaaagaaaa agccgaagcg gaacgtttag cggcggaaaa agcagcgaaa
    19861 gaaaaagccg aagcagaacg tttagcggcg gaaaaagcgg cgaaagagaa agcggaagca
    19921 gaacgtttag cggcagaaaa agcggcgaaa gagaaagcgg aagcggagcg tttagcggcg
    19981 gaaaaagcag cgaaagaaaa agccgaagca gaacgtttag cggcggaaaa agcggcgaaa
    20041 gagaaagcgg aagcggagcg tttagcggcg aaaaagcagc gaaagaaaaa gccgaagcag
    20101 aacgtttagc ggcggaaaaa gcggcgaaag agaaagcgga agcggagcgt ttagcagcag
    20161 aaaaagaagt agatcctata gctattatta aagcaaaagg tattgatgat acaaattacg
    20221 gcttaaaact tggtaatagt aaagaaacta ttgagggtaa agtcacagtc agaacacaga
    20281 gttatttata taatcagcca tattctgtag taacagcaca agcagttcaa agaaatggct
    20341 gggaaaacgg taattatatt gataaagttg atggaacagt tacagttaaa ggattgaaaa
    20401 cagagaaatt accaacagag ggtactgcaa catattcagg aaaagcattt aaccacgatg
    20461 gagctaaagg ttttaatggt ggtgatctaa cctataatgt taatttctct aagcgtaccg
    20521 gttcaggtag agttgaggga gaatttggaa acttaatcgt attagaagag gggacgatta
    20581 ataatactca gatttcttca acagtacatg gcaataatcc tttaaacctt tctggaaaat
    20641 atacagtcga atttttcggt aaaaatgcag aggaaattgc cggtgaaatt cagacaaaag
    20701 caaatcttac tggggaagtg agtaattttg gacttgccgg cactcgtggc gatatccaaa
    20761 aataaccttt tctaacgttc aatgccgttt aacgaaaaat taaacggcat ttgtcttgat
    20821 attgcttgta gatacaataa gacacaatag tttagatttc aatttacagc ttctaataac
    20881 cctctcccac aaggggagag agtgataaat cccgtagagg cgaacatata ggtccgcccc
    20941 taccaatttt ctttatcttt atgaaaaaat atcccctttt attttcgctg ttcagccccg
    21001 ttgtatgggc agaaaaggtg attgaacaca acactgaccc gatttccttt gagcgtcagc
    21061 cgcaagcggt taaatttgag caaaatgttg taaaaaattc acaaaaaccg accgcttatc
    21121 agcctgcgta ttcagtcaat cagaatgttg aacagcaaat taaccaagcg attctcagcc
    21181 aagattgggc aacgcttgaa ccgcttttaa gccgttatcc gcaaactgaa aattttgacc
    21241 cgttgttgtg gcactatgcg caagccgcac ttgcctatgc gaaaaaagat catcaaaccg
    21301 cagtttttca ctatcgccaa ctgttagcta agcccccgag tttggtttac cctcgttttg
    21361 atttggcgtt gattttggca gaagatcagc aatttcgtgc ggcagagcag gaatttaagg
    21421 ctattcaagc ccatttaccg ccggaacttg ctaaaattgc ccgcaatact caagtccaaa
    21481 ttgctgaaaa tgaggcgtgg cagcccgatt tttatctgca atatacccaa acagataacg
    21541 tgaataatgc ctcatcttcg ccgattgtga atgtaaatgg gcggattttc cgcaaaaatg
    21601 cagacagttt gccacaatca gcaaaaggcg tgcgttatgg cttagggttg agtaaattgc
    21661 acaacgtgaa aggcaatcat tttgtcggtt taacgctcaa ttatagcggg atttactatt
    21721 gggataaccg agattaccgt gagcaatccg tatccgtttc gccgaattat agctaccgca
    21781 ccgtcgctta tcggctgact ttttcgcctt ttattgagca aaattggctg gggagtagcc
    21841 gttataatgt tcaatttggg gcaacactca acggtttgcg ttaccttaat tcgcaatggt
    21901 tggtgtcggg gagttttacc catttgcaaa aacggtattt tgaggagcta accgcaagcc
    21961 gttataacag ctatcagcac caactcagtg gcggtgtgca gtggcaagcg gtcaaaaatg
    22021 ggcggttttt tgcaaattta gacggcgtgc gtgaaattgc acgggaaaaa gcacaaagct
    22081 cgaataagtg gctggctcga gtgggggcgg tttttcaagg cgaacgctgg gcaacgcagg
    22141 caagtatcgg ctttggcaag cgatattttg ccgacaagca ctatttgttt ggctataagc
    22201 ggttagacac ggaatatcaa gccaatttag cggtttggaa ccgcacttgg cattggcagg
    22261 gcattgtgcc gaagctgaat ttccgttatc aaaaaattga cagcaatatt gctgattttt
    22321 actctcgcca aaacaaggaa gtctttttca ctttagaaaa gctattttag tgttggtttt
    22381 ttgtgctaag catacgattg cgtagctgcc ggcacaaaaa aataaatgaa atgatggcga
    22441 aaaaagcatt ttcgggttat cctagtgcaa ttttttatat cggggtagcc cccaatttta
    22501 tattaggttg tttctatgag tgaaaaatac acaaatgaaa aatatgtcgt gacgtgggat
    22561 atgttccata tgcacgctcg caagttggca gaaaatttat tacctgccac acagtggaaa
    22621 gggattattg cggtgagccg tggcggcttg tttccggcag ccgttttagc acgtgagttg
    22681 ggtattcgcc acgtagaaac agtgtgcatt gccagctacg atcacgatag ccaaggtgag
    22741 ttaaatgtgt tacacgcagc cgataccgat ggcgaaggct ttattgtggt agatgattta
    22801 gtggataccg gcaacacggc aaaagaaatt cgcagaatgt acccgaaagc tcgttttgta
    22861 accgtgtttg cgaaaccgca cggtgcaccg ttggttgatg attatgtgat tgatattcca
    22921 caagaaacgt ggattgagca gccttgggat ttaggcatta ccttcgttca gcctttggca
    22981 cgtagataat tttgtgtaat ggacaatgaa agttgtccat tattttttat ttattcctat
    23041 gaaatgtagg ggcgaatcat cattcgccct attcccaaaa tcaaataggt atatacaaat
    23101 aatgagttta ggcaatcttt atattatttc tgccccaagt ggagcaggaa aatcttcctt
    23161 aattaacgcc ttgttggcgg atttaccccg ttcggaagta cagctttcga tttcacacac
    23221 tacccgtcag cctcgtccgg cagaacagga cggcgtgcat tattatttca cttcgcacga
    23281 tgagtttaag cagctgattg agcaagggta ttttttagaa tgggcggagg tgtttggcaa
    23341 ttattacggc acatcattgc cgatgattga acgtagccta gagcagggca ttgatgtctt
    23401 tttggatatt gactggcaag gggctagaca aattcgggaa aaagtgccga atgtgaaaac
    23461 cgtttttatt ttgccaccgt caaaaacaga attggaaaaa cgtttaatcg ggcgtgggca
    23521 agattccgct gaaaccattg ccaaacgtat ggcagaggcg gtgtcggaaa tgaaccatta
    23581 caatgagttc gattatgtga ttattaatga tgatttccaa caggcattgg cagagctaaa
    23641 agcgatttta attgccgagc gtttgagctt aggttcgcaa caaattcgtc atcagcattt
    23701 gattactgat ttgttaaata attaaatgaa cagttgttca tttaactgga aaggcaatct
    23761 gtttttgggt agaatacgcc cattttcccc tctttatttt caaaaggaaa tgtaatgaaa
    23821 ctttcaaaat tagcattttc tgtggcgttg gctctaaccg taacggcttg taccacaggg
    23881 aatatgagca atgacgggac tttgcaacaa gcacagcaaa cctatcaaag ctatgatgaa
    23941 attaccaaac aatatcaaat taacgagcaa tggtggcaag gctatacgga tgcggaatta
    24001 aaccgtgtgg ttgatttagc catcaaaaat aaccttgatt tagccaaaag cggaattgca
    24061 gtcaataaag ccttgtataa cgctaattta gtaggggcaa atttagtgcc aacctttagt
    24121 ggttcaggtt ctgtttctgc ggctaaaggt ttaggttcgg cttcttcaaa taatgtttcg
    24181 accggtgttt caacaaccgc agctcaagct gcatttaatt taagttatac tgtggattta
    24241 tggcgacgtt tagccgatac cgccagtgcg gcagaatgga cgcataaagc caccattgaa
    24301 gacttaaaag ctacccgttt atctattatt aatgcggttg tgactactta ctaccagtta
    24361 gcttactatc gtgatgcgat tcgtattacc gaagagagca ttcgccatta tgagcaaatc
    24421 agccgtattt taactaacaa aatgaatgcc ggtgcgattg atagtttagc ggttgatcag
    24481 gctcaacaag cggtcttaaa cgccagaaat agtgtaattg atctgaaaac caaccaaaaa
    24541 attgccgagc aaacattacg caatttgtta aatgttcagc caaatactcc gcttaacatt
    24601 cgttacccga atattatgag cgtgaaattg caaggggtag atttaaatgt gccagtgtca
    24661 gccattgcta atcgccctga tgtagtaggc tcgttacaac gcttacaaag tgcatttaaa
    24721 accttaaccg caaccgagaa aagctggttc ccaaccttaa ccatcggtgg ttctattacc
    24781 ggtagtgcaa ccaatatcgg caatgtggca gataaccctg tgggtaacgg cttaattaaa
    24841 ttggatttac cgttcttaga ttggaatcgg gttaaaaata acgtaaaaat ttctgaaacg
    24901 gattatgtga ctgcccgttt aaactatgag caaaaaatta cctcagcctt aaatgaaatt
    24961 gaccgttatt attacagtta tacgcagtcg agaagctcgt taaaaatcct tcaagatacc
    25021 aatgcgaaaa ataaacgtat cagttcatac tataaaaacc gttatgaaca aggggtgtcg
    25081 gagttccgtg agtggattac cgcactgaat accgagctta attctcagtt ggcgatttta
    25141 aatgcaaaat atgcgatttt gaccaacgag aatgcggttt accaagcaat ggcaggtaaa
    25201 tacagaagat aattatatct aaaactatat actatggttg gtaaaagtgg gttaaggctt
    25261 gatggcttaa cccgcttcgc tatttattgc tacttaaacc attttggtaa tgttagttta
    25321 gctactagaa cttaatcaca tcaccgtttt tcggcacagt tacacgattt aatccctctt
    25381 tttgtacata atcacgcatc tgatcactgc tgattgaggt ttgtggcagg gcgtccatat
    25441 gcacagttaa gattttcgct tgtggagcga ttttgtgtgc tttggaaaca tcttcggtac
    25501 acataatcgg gctgccttca aagtttggta gtttggcaca gccggcattc agcaccacaa
    25561 agtctggttt gtaagtgttg aacgaatgtt cgatttcgtg gttccaaatg gtatctccca
    25621 ccaaatagac ggtcggtttg ccttccgctt tcatcactac gtccatcgca tcgccgacca
    25681 tttcggcaat tttcgggttc gcatacatcg catcggtgcc gtgctggcca cgagttcggg
    25741 ttaaggttac gccttcaaac ggggtatctt tgccgaccac acgcacatcg gtaaagcctt
    25801 gtgaacggat taaacgggca tcaccggcat tctgcacaaa aatcggcaga tttttcggca
    25861 cacgttgttg tgctacatcg tcccagtgag cgtggtgagt gtgagttacg atcaccgcat
    25921 ccacgccatt taacacttca tcaatgctca cagtcatatc caaggtcgga ttacgttttt
    25981 tcgcattcgg cggtaagtag cgatctttct ccgctaaata cggatcaacc aaaaacgtct
    26041 tgccggcata ttcaattttc gccgttgcat tacgaatatg ttggtattgg ctctgctcag
    26101 cggcaaaact tgatgtagcg aaaagtgccg ttgcaatcag gctcagtgct gtttttttga
    26161 caaaagtcat aggttctcca aaataaatta aaatgcagag tatgattttt tacgctctgc
    26221 aatgctggtt tgcataagga acagtgtccc ttacatcaat aaaagcggtc gattttgcac
    26281 atttttctgc aaaaccaacc gcttgttcgg ctaggctaat ttctctacct tcgccggaat
    26341 gccatttctc accgctgagc cacagaccca atctacccac ggggatttga ccgctttggt
    26401 gtggtcgata atgccgaggt cgttgaggtt aatccctttg gcgatcatcg ggttggctgg
    26461 aatttccacg ccgtcaatgg tgtaggaact tgccccaatc tgtttatgac cgtagccgtg
    26521 ttcgatgccg attgtgcctc gggcgatacc gtccaacacc acaatctgca ccgtcaagcc
    26581 ggcggttggg gtgctgagtt tcaccacatc gccgtgttta atgccttttt cttccgcatc
    26641 taaacggttc atcgccacta agccctcagg cttgatcgag agcagacgca acagtggggc
    26701 ggtaatactg ctcataatgt tcgatttaaa cgaaatcagt ttaaagcccc attccgactg
    26761 cggatagtgc tgctccagcg tggagccgtc cgcaaaacgt ggcgacatat aacgagggca
    26821 gccccaatag tgttcaccgt tgctgtggtg ctgggcttta tacacgtttt cgttccaaat
    26881 ttgcaggcag tttttccact ggaactccat attctcctct ttccacgcac tttcgtgtgg
    26941 ggcaaaacgt ccgcctttgc aatacacatt cgccactttt aatcgctctt cggcttttag
    27001 cgtttgttca attttcggca taatccgttg cacgccggtg agcagcagat cttgctcgct
    27061 ggcgtccggt gcaggtttct caccggcaaa ggcaatgttc gctgccgccc gtaggaagta
    27121 atcttcggta cggtggagcg gatagcggtt gccttctaaa tcggcaatcg cattttcgcc
    27181 aaagcccggc aagcccattt ttttcgccac cgcaatcacg aaattctcca tacagatggt
    27241 gtcgccttcg gcagttttgg cgtttttgga cggcacaatc gggtggcgtg cggtgctggt
    27301 gcgttgaggc acaccgcccc acgccgttga aaagccccag ctctcaaagt tgtgggtgtc
    27361 cggcacgatg tagtccgtca gtgcagtggt ttcgttcata aaagcatcaa tagagacaaa
    27421 gagcggtaga attttcggat cttttaattt ctccaacgca atcgggttca tagcggttaa
    27481 gccgtaaacg gggttcgcca tatggttgat ccacgctttc gcaaagtatg gatagccctg
    27541 caccattgag gtaaacatct cggtagtttg cccgccggtg aacggatacc acgccccttg
    27601 tgccggatac ggattttcgc ccttcgtcac tttctgctta aattcgcttg aattttggta
    27661 cgccttttta ctgcgggcaa ggttcgtgcc ttttggcttc acttcattcg ggaagttcgc
    27721 taaattgtag cgaggcccgt ccccgaaatc tttgaatttg cctgcggcta ctgccattcc
    27781 gccttttttg ttgaggttac cgaccattgc attcagcaac aaaatcgccc aactggtata
    27841 aaaaccggtg ctgtgcatcg tgccgccgtg agtgatggca ttggctttat gaccgtgcga
    27901 agtgaatttt ttcgctaagt tttcaatggt ttgcaccggc acgccacaaa tgtcgctata
    27961 ttcggcaagg gttttctctt gtgtcgcctc tttcagcaac tgcattgagg atttcaccaa
    28021 cacttccgag ccatcaagca gttttgcaat ttgttcgaca aaaatgaccg cttgtaagca
    28081 atcttttgct gaaatgagtt cgccgcttgc ctgatctttt accacaatca ggttttcttc
    28141 cactttctct tcggggtcga gttgcacatc ttgcagatcg ctaatccgca acgcctgccc
    28201 gaatttcggg tggttcggtt cgcaaataat cagatgtgac gcattggcat agctgacgtg
    28261 gccggcattt tccatcgctg ccagacttgg gcgggagagg tagtcagcat tgaaacgctc
    28321 gttttcgata atccaacgca acattcccat taccaacgcc aaatcgccct cgggcttaat
    28381 cggcacccaa tggttgtttt gggtggcgag ggaggaggtc atctccaaac gaggggaaac
    28441 caccacatag ctgaaattgt catcagggcg gcgtttggca agctggcgag cctgacgctt
    28501 aaacggattg cccgactgtg ccggagacgt gccgataaac agaataaatt cagcgtgatc
    28561 ccagtccggc ttggcgtgtt gttggttgtc aaaatctttc ataaaggcac ccgaaccggc
    28621 acggtacgca gcgccacaat aagccccgtg gttggaaaag ttgatcgtgc cgaagctctt
    28681 ttgggcaaag cgttgcaaaa tcggctgacg accttcgggg ccggcaaaag taactaaaag
    28741 ctggtttgct ttcattccca aatcaggctg tttcgggtta atcggatcga ccagattgcg
    28801 gatcgtcttc aagccctcca cttcgccttc accaaatagg ttaccaccat tgatgatttc
    28861 ctccaccaac tgctcaaagc tgatggtctg ccacttgcct tcgccccgtt ttccgacccg
    28921 tttcaacggt tgggtaatcc gatacgggct gttcaccccc tccaagaacg ccgcccctct
    28981 agcacaggcg gtggagcgat tttcaatgcc actttcgccc gccagcaaca gttccgcctt
    29041 ggcaatcggc ataccgaacg gcagggtttt gtcggaggaa agcgggtgat acggattgcc
    29101 cgaaatgcga atcacttggt tggtgttcaa atccaccctc gcccgaatgc cacactgcgt
    29161 ccaacagccc atacactgtg tattgcacac aatttgctca ggattgctga gcagtttgcc
    29221 gtcttttacc tgatactccg gcgtgagcga attaccgtga attgcatcac gggttttctc
    29281 gcccgatgtg ccgtgcatca cccctttggc aatctccgac accttcggcg aatacccggc
    29341 cccaaacgcc gccgccccac caagtgccag cccgcctttc aacagatttc tgcgtttatt
    29401 gttcaccttt tcttccccca caaattatta aaatcagttg ccataccacg atacttacta
    29461 gcaccgttag cccaaaaatc gagacaatcc ccaaaccacc atcgacggct gtccaatcga
    29521 aatgatatgg attttgcaag gcattggttt tggcaatggt ttggacttgg atcaccagta
    29581 cccagcggag tagccagccg aaagcgatga gggcaagcac gccgagccat tgcaagcggt
    29641 ggtttttggc agaaaaattg caaatgaggg caattagcca gaaaatgccg acaaggtaag
    29701 cggtgattga gccattgaag agctgggcaa aatcttgtgt ggcttgtgga ctactgaatg
    29761 ctagtccgac aagcgaaagt gcggttaaaa tcaggcagag ttttgtccat agccccgtgt
    29821 tggtttgggt gtggaacagc attttgcaga ccgcttgggt aagccccaat gccaccgccc
    29881 acgcagaaaa gagcataatc ggcagcagcc acgcactgtg ccataaggtt cttgtgccgg
    29941 tttggtaggt ttcaagcacg gtgtagccga taatgccgat agcggtgagt gtggcaaaca
    30001 ggcggaatgc ccaaagcaag ccttgatttt gcagtatcgg caactgtagc cacgctaacc
    30061 atttcggcag gttcggccgg gtatggtgag ccaatgccag cacaaaataa cccgctaccg
    30121 ccacgctgaa caggggcagg ataatcgctc cccacgccat tggagatcgc cacgcaaaat
    30181 cggtgtagaa atgcagaata cggctcggtt ggtgcaggtc ggcagtcaat gccacagggg
    30241 cgacaatcga gccaatcaag gctaccgtca ccgccgccag ctcgcaagcg gtcggtttca
    30301 gtgatttttt tgcaaacagc acggcggtaa acactgccgt tgccgccacg cctacgaaga
    30361 agaaataatg cacaatccac ggcaaccaga cgatatgttg cggttccact aatacttcac
    30421 gaatcatttt acgcctcccc cgtccagagc attggttgcc cgtcaatttt gctcacaaag
    30481 gcatcatcgt ctagccccac atagaaaacg tgtggcacgg tgccggcctc cggtttcagg
    30541 actttcaaat catctttgtg ggcttgataa agttgggaaa tttgactttt cgggtcgttc
    30601 aaatcgccaa tcacccttgc cccgcccacg caactttcca cgcacgccgg cagcagacct
    30661 acctccaaac ggtgagtgca gaaggtgcat ttatcggcgg ttttggtctc ttcattgata
    30721 aaacgggcat catacgggca ggcttgcacg cagtaaccat agccgatgca ctgctcattg
    30781 ttaatcacca ccacaccgtc tttacgttga aaggttgcct gcaccgggca gaccggcaca
    30841 cacggcggtt gatcgcaatg gttacacaaa cgaggcaaca ccacattgtt caccacctgc
    30901 gtgccgttgg tgatttcata ttgacgcacc gtggtgcgga aactgtgcaa cggcgtggcg
    30961 ttctccaccg aacagctcac ggtacacgct tgacagccga tacatttacg caaatcaatc
    31021 agcatcgcat agcgtttgtt ctcatcgccc tcacgccaag ccggcataaa cgcctgggcc
    31081 gattgcaacg gcacaaaact cgcccccacc gtcaaggcgg aaagctcttt caaaaaggaa
    31141 cgtttgctta aatccatatt tcccccacaa atttaaaaat aacaaaggta ttctaaggcg
    31201 gagagggaac ggcaaggtat tgtggttttc cacatatcaa ggggaaagtt gatttgggtc
    31261 aaggatgaag ggaaatgccc tatacaagta gataataggg cattgatact aattaaaaag
    31321 caataagatg gtttagttta gatctaaagt ttttatacgc attaatatca ctcggggtac
    31381 taggtatcgc taatctccta gaatttgggg caattaatat attatgtttc tttccttttc
    31441 taattttcca tcctgctttt aatttagata taacaatact atttataaga gaatcattac
    31501 tgtatttctt catatatccc cctacaaatc gtagcataat ctaaatccta ttttggtata
    31561 tttatatttt cctgaccaat ccaatggtgg aacggccctt ataacagact tgtcgccata
    31621 aaagctggtt aatgttttag agcgaacaca agccttttca agaatccatt ccgcaaatct
    31681 gtcagattct atgaagttta tgtggttgtg catc
//
LOCUS       DANLZC010000007        31063 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.007, whole genome shotgun sequence.
ACCESSION   DANLZC010000007 DANLZC010000000
VERSION     DANLZC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 31063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31063
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.007"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            6..296
                     /locus_tag="PXE44_000296"
     CDS             6..296
                     /locus_tag="PXE44_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5587843.1"
                     /translation="MKIYTWDETKRKINLAKHGLDFADADEVLSSPYRFDVEVIRNNE
                     FRIQSFAYSFGRLLVLSLAYTEREDYIRVISFRRASKAEREAYYEYLQQYFE"
     gene            268..570
                     /locus_tag="PXE44_000297"
     CDS             268..570
                     /locus_tag="PXE44_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL5587844.1"
                     /translation="MNTYNNTLNNKNLEKKQLLMAIQAIPASQDFIWDGKDDDERPVT
                     PSEFSIAMKKRGRPAGSKKELVSLRLDKSVLEAFRASGKGWQSRINQVLSDFVKQV"
     gene            complement(588..881)
                     /locus_tag="PXE44_000298"
     CDS             complement(588..881)
                     /locus_tag="PXE44_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HDL5587845.1"
                     /translation="MTQLSTSVYQRADGFINVANAHLKNIAPNQVSNAMLFGCARFNA
                     YVAASKAEYKQQLADSREEVIQYFVEQYKEMLTANLDEYIQNFERYIEGKKAD"
     gene            complement(1047..2006)
                     /gene="rpoH"
                     /locus_tag="PXE44_000299"
     CDS             complement(1047..2006)
                     /gene="rpoH"
                     /locus_tag="PXE44_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDL5587846.1"
                     /translation="MKKFNENEIEDAEIIDAEPIDVDEIDEEETSVEAMHPALVSGSM
                     PVPQGNLDSYIRMANQYPILTAEQEKELAERYYYDEDVEAAKQLILSHMRFVIHIARG
                     YLGYGLPLADLIQEGNIGLMKAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVK
                     VATTKAQRKLFFNLRKNKNRLAWFNEEEIKKVADDLGVSVEEVREMESRMTGQDLGFD
                     LPAGDDDEDSYAPSMYIEDSSSNFADDLEDEQHNGQATAQLAYALATLDERSQDIIKT
                     RWLDDNKATLQDLADKYNISAERVRQLENAALKKIKEAISLDG"
     gene            2172..2538
                     /gene="ssrA"
                     /locus_tag="PXE44_000300"
     tmRNA           2172..2538
                     /gene="ssrA"
                     /locus_tag="PXE44_000300"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2813..4039
                     /locus_tag="PXE44_000301"
     CDS             2813..4039
                     /locus_tag="PXE44_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5587847.1"
                     /translation="MARKITPLNDTQIRKAKPEDSPLRDGNGLLLVITSNSKLWRFRY
                     ERPFTKKRNDLSIGAYPDISLAQAREIREEYRALLAQNIDPQTHRQQQEQAKANEISN
                     TYENVAWAWLEHRKTKKNFSDNYQKDVIALIKNNLLPHFGRMPITQITAPMAIKAFKQ
                     YQDEGKLEKLKRAIQKHNEIMMFAVHREMIPFNPTANIAKEFDSPTVEHFKTIKPEDL
                     GEFLYTLQNAQIHLQTRYLILWQLLTMTRPNEAAKAKWADIDEKAKLWTIPAENMKRG
                     IEHKITLSRQAIALLREIKKLSGGKTYLFPSVKNPQTHVNTQTANGAIKRMGYKGKLV
                     AHGLRAIASTYLNEKGYNSDLIEVALSHLNSDRIKAAYDRGERLEQRFKLLQVWADFV
                     EECSQGSLPQYHLKIA"
     gene            4314..4532
                     /locus_tag="PXE44_000302"
     CDS             4314..4532
                     /locus_tag="PXE44_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module killer protein"
                     /protein_id="HDL5587848.1"
                     /translation="MMREQTTNQIIITDNFSKWLENLKNPIAKATVARRIKNAINGNF
                     GDHKSLAGTGGLYEMRIATGAGYRIYYA"
     gene            4645..4968
                     /locus_tag="PXE44_000303"
     CDS             4645..4968
                     /locus_tag="PXE44_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5587849.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNDVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
     gene            5363..6049
                     /locus_tag="PXE44_000304"
     CDS             5363..6049
                     /locus_tag="PXE44_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587850.1"
                     /translation="MADHSQFISRLENRIEPKISFQELIQELSNYTNKPFSHIADKLK
                     SLIFSFQQNGIYTNAFVNCTLFEDGESYAIEPKTLHFKDAFDQPKGVLSDVIAQNSID
                     VEHLNAYYVTAREITQLIKTQSPTLLDKLTTKQTQSEISQIQNDYISVYDFIEWASKH
                     YSSLSETANDLNRLLKDKNIQLYRQYGGISPSIDKDNTNLKAAFDFVAINNGYEKQSS
                     SFNFDDDIPF"
     gene            6210..6362
                     /locus_tag="PXE44_000305"
     CDS             6210..6362
                     /locus_tag="PXE44_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587851.1"
                     /translation="MPELLIVLGNRIAKLEQQHNAMPSETDSITLGILRKQLSQLLKV
                     AERIKE"
     gene            6369..6794
                     /locus_tag="PXE44_000306"
     CDS             6369..6794
                     /locus_tag="PXE44_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587852.1"
                     /translation="MKHELNLAGITALMNEPIKATLSFLDFPHIRIERLHHDNGRPFF
                     IGVLSLDLEDHINIEMAGATGFLEALAIIAYHQQLTATPLLVNDDLLSEINKLPHPQR
                     TNPFIDRHGNEITREQMSKEYREKIDYAFAEKRMAEGKE"
     gene            6791..7483
                     /locus_tag="PXE44_000307"
     CDS             6791..7483
                     /locus_tag="PXE44_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL5587853.1"
                     /translation="MTELTPKQEAFCFAYIETGNASEAYRQAYETEDMKSETVHRKAK
                     ELMDNGKITARIEELKAEHAERHKLTVDDLLKELEEARILAREKENPNAMTQATMGKA
                     KLLGLDKQVTDPCFDGETGKGDMILSVNCSSIKDGKALDETCILKAGTHEFITRDSFI
                     FYRHIRIDSLDEIKANIEAGYFIQKALINDEAYQQILKGVLSSKSVTPRYKRFLKNAI
                     RQSACPDILAEP"
     gene            complement(8023..8634)
                     /locus_tag="PXE44_000308"
     CDS             complement(8023..8634)
                     /locus_tag="PXE44_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5587854.1"
                     /translation="MQLTFNDFQNYYNGKGDLTMFSFYGKKTWENGIYVGIDGSIGRA
                     NNKLSHQSQTIKLARNVYSLGLTVGKTLEVASFNLQPSFGMRYHHLTSANAQLENTKF
                     ETDRVDLLAIQAGLALNKTFIFNELKVKPELGSYFVDASHGTLKTKLNEFSLNQNIGR
                     YFKQEVGITLHYKGVSSSVHAGFTKGNTLQEQKFISLKVGYEW"
     gene            complement(8683..9498)
                     /gene="cysE"
                     /locus_tag="PXE44_000309"
     CDS             complement(8683..9498)
                     /gene="cysE"
                     /locus_tag="PXE44_000309"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL5587855.1"
                     /translation="MQQSEINQIWQAIRDEAKELANCEPMLASFFHATILKHHNLGDA
                     LSYILANKLENPIMPAIALKEIIEEAYRAEPQIIASAACDINAVRTRDPAVDKWSTPL
                     LYLKGFHALQSYRVTHYLWNQGRKALAVYLQNEISVAFDVDIHPAAKVGCGIMFDHAT
                     GIVVGETSVIENDVSILQGVTLGGTGKENGDRHPKIREGVMIGAGAKILGNIEIGRYS
                     KIGANSVVLQPVPDHATAAGVPARIIGKSSEQKPAFDMNQYFEDEQGLFGDGI"
     gene            complement(9514..10524)
                     /gene="gpsA"
                     /locus_tag="PXE44_000310"
     CDS             complement(9514..10524)
                     /gene="gpsA"
                     /locus_tag="PXE44_000310"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL5587856.1"
                     /translation="MSEIYSAPITVLGAGSYGTALAIALSRNGHKTYLWGHNPDKMAV
                     LASERMNQDFLPDIVFPEALEIVADLATAVQKSKDLLIVVPSHVFSEVLAQIKPFLSQ
                     DHRIMWATKGLERDTGRLLQTVAKEILGEIYPLAVLSGPTFAKELAAGLPTAISLAST
                     DPVFADQMQKRSHCSKAFRVYLNNDIIGVQLGGAIKNVIAIGAGISDGMGFGANARTA
                     LITRGLAEISRLGVSLGADPKTFTGMAGLGDLVLTCTDNQSRNRRFGLALGQGKSAEV
                     AIAEIGQVVEGFYNTKEAYLLAQTQGVEMPIVEQIYQMLFCGKQASDVVKTLLGRERK
                     GE"
     gene            complement(10631..11131)
                     /gene="secB"
                     /locus_tag="PXE44_000311"
     CDS             complement(10631..11131)
                     /gene="secB"
                     /locus_tag="PXE44_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDL5587857.1"
                     /translation="MSEENQVASTEEATQTPFELQIQRIYVKDVSFEAPNLPTIFQQE
                     WKPQLGFELDTETKEIGEDLYEVTLHITVQTTLEDSNDVAFICEVKQAGVFTIKGLEG
                     IQLAHCLASQCPNVLYPYARELISSLVNRGTFPALNLSPVNFDALFMDYLQRQEESAE
                     EPALAN"
     gene            complement(11152..11604)
                     /locus_tag="PXE44_000312"
     CDS             complement(11152..11604)
                     /locus_tag="PXE44_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL5587858.1"
                     /translation="MEQLTFAQQLEQFFANHTLMVFAWVALLIAVIFSFYKSATQKYK
                     IVDNAQATQLINKEEAVVVDLRSDEEFRAGHIIDSIHVLPSDIKAAKINQIDKYKARP
                     VIVVDTNDFTSGGVAADLAKQGFSRVYVLKEGIGGWRAASLPTIKKHK"
     gene            complement(11720..13018)
                     /gene="tig"
                     /locus_tag="PXE44_000313"
     CDS             complement(11720..13018)
                     /gene="tig"
                     /locus_tag="PXE44_000313"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL5587859.1"
                     /translation="MSFSIETLEGLKRRVTINVPADKIEAAYKEQLKGYAKNARVDGF
                     RKGKVPHSIIEQRFGIAARQDVLSDEMQRAFFEAVIAEKINLAGRPTFTPNNYQPGQD
                     FSFTAEFEVFPEVELKGLENIEVEKPVVEIADADLDKMVDVLRKQQATWSESQEAAKA
                     ESRVTIDFVGSVDGEEFEGGKASDFVLFMGQGRMIPGFEDGIVGRKAGEQFDIDVTFP
                     EEYHAENLKGKAAKFAITLKKVEEMVLPELTEEFVKKFGNAKSVEELRAEIKKNMQRE
                     LKNAVTSRVKTQVINGLIAQNDVEVPAAAVEEEINVLRQQAVQRFGGKPEMAAQLPAE
                     LFEAEAKRRVQVGLLLSSIIGSNELKVDEKRVEEMIADIASAYEQPAEVVAHYAKNRQ
                     LTENIRNVVLEEQAVDAVLAKAKVTEKTMSFDEVMSQQAQ"
     gene            complement(13315..14499)
                     /gene="tuf"
                     /locus_tag="PXE44_000314"
     CDS             complement(13315..14499)
                     /gene="tuf"
                     /locus_tag="PXE44_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL5587860.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKHFGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTATRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALQALNGVAEWEEKILELANHLDTYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIRTGDEVEIVGIKDTAKTTVTGVEMFRKLLDEGRAGENVG
                     ALLRGTKREEIERGQVLAKPGSITPHTDFESEVYVLSKEEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMTVSLIHPIAMDEGLRFAIREGGRTVGAGVVAKI
                     IK"
     gene            complement(14559..16661)
                     /gene="fusA"
                     /locus_tag="PXE44_000315"
     CDS             complement(14559..16661)
                     /gene="fusA"
                     /locus_tag="PXE44_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL5587861.1"
                     /translation="MARTTPISLYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMSQQYPQHRINVIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRTGANFLRVVEQIKT
                     RLGGNSVALQLPIGAEDSFKGVVDLIKMKAINWNEADQGMTFTYEDIPADMLEACEEH
                     RAMLVEAAAEASEELMEKFFSGEELTEDEIKAALRQRVLAGEVIPVCCGSAFKNKGVQ
                     AMLDAVIDYLPAPTDIPAIKGINEDETEGERHASDDEPFASLAFKIATDPFVGNLTFF
                     RVYSGVINSGDTVYNSVKQKRERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVGTG
                     DTLCAIDAPIILERMEFPEPVISVAVEPKTKADQEKMGLALGRLAQEDPSFRVHTDEE
                     SGETIISGMGELHLDIIVDRMKREFKVEANIGKPQVSYRETIRTRVNDVEGKHAKQSG
                     GRGQYGHVVIDLYPLDAEGPGYEFVNEIKGGVIPGEYIPAVDKGIQEQLKSGPLAGYP
                     VVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAAFAKASPVLLEPIMKVEVETPPDYV
                     GDVIGDLSRRRAMVNGQEANEFVVKINAEVPLSEMFGYATDLRSQTQGRASYSMEPLK
                     YAEAPKNVADAIIEARRK"
     gene            complement(16763..17233)
                     /gene="rpsG"
                     /locus_tag="PXE44_000316"
     CDS             complement(16763..17233)
                     /gene="rpsG"
                     /locus_tag="PXE44_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL5587862.1"
                     /translation="MPRRRSIEPRKILPDPKFGSELLAKFINVLMVDGKKSTAEKIVY
                     GALETLTQRSGKEALEAFEIALENVRPTVEVKSRRVGGSTYQVPVEVRPTRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAADNKGSAVKKREDVHRMAEANKAFAHFRW"
     gene            complement(17364..17738)
                     /gene="rpsL"
                     /locus_tag="PXE44_000317"
     CDS             complement(17364..17738)
                     /gene="rpsL"
                     /locus_tag="PXE44_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL5587863.1"
                     /translation="MATINQLVRKPRVKKVVKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRIRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGAL
                     DCAGVKDRKQGRSKYGVKRPKA"
     gene            complement(17858..18817)
                     /gene="rfaC"
                     /locus_tag="PXE44_000318"
     CDS             complement(17858..18817)
                     /gene="rfaC"
                     /locus_tag="PXE44_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDL5587864.1"
                     /translation="MKILIVKTSSMGDVLHTLPAITDMQNAIPDLKVDWVVEENFTEI
                     PAWHSAVNKVIPVAIRRWRKNLCQRQTWVEWQQFLKQLKSEQYDAVIDAQGLIKSAVL
                     VTRQAKGKKFGYDKNSAREGLSSLFYDETFNIPYQQHAVERIRKLCAKSLGYALPTER
                     GDYCIAQHFAKNPQKTTAYIMLIHATTRADKHWQEAYWAELIQALDKHNLAVRLPWGN
                     QAEKERAERLASVANNAKVLPKMSLTDIATQLAGAAAVVSVDTGLSHLAAALDKPNVI
                     LYGATDPKLIGAYGQNQFYLKADKMENITSSQVLEKLLPLINK"
     gene            complement(18884..19921)
                     /gene="waaF"
                     /locus_tag="PXE44_000319"
     CDS             complement(18884..19921)
                     /gene="waaF"
                     /locus_tag="PXE44_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HDL5587865.1"
                     /translation="MNILVIGPSWVGDMMMSHALYQQLKIQYPNCQIDVMAPDWCCPL
                     LARMPEVRNAISMPIGHGSFRLCERYRLGKSLRNQYDMAIVLPNSLKSAFIPLFAKIA
                     RRRGWKGESRYFFLNDLRSNKNDYPMMVQRYVALAFEKATVPTAQQLPIAYPYLTTDS
                     EQIEQTKAKFEKQLAYAENRPAIGFCPGAEFGPAKRWPHYHYAILAKMLIEQGYSVRL
                     FGSKKDEAAGEQIRLALPENLQRYCLNLAGQTDLNQAVDLIADCNAVVSNDSGLMHIA
                     AALNKPLVALYGPTSPQYTPPLSQNAEIMRLIEGGLIKIRKGEGAEGYHQSLIDIQPE
                     MVMDKLAELLV"
     gene            complement(20032..20394)
                     /locus_tag="PXE44_000320"
     CDS             complement(20032..20394)
                     /locus_tag="PXE44_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HDL5587866.1"
                     /translation="MLVACSTVVTPVEQIHQVQKEKESVKKVTKLIAQAPKVSLYRCE
                     NDKKVEVERQGRTKKALKKEIVTVSYQGTTHQLSPSVTRDGKKYTNIRWTWHETRSGK
                     AFLYNNTKKTLAANCIKQ"
     gene            complement(20512..21831)
                     /locus_tag="PXE44_000321"
     CDS             complement(20512..21831)
                     /locus_tag="PXE44_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="opacity-associated protein A"
                     /protein_id="HDL5587867.1"
                     /translation="MTQNNFRKEPVFGESSVTSQDAEATKTDTAQAPYVSLRSTKAPG
                     HTFTPIMKRPDAKVEDHTADVFSAKQAKEFQFTPSSEATEAVEPDNTIHSVEESVSTA
                     SFNAVEAQENVEKEVVKPAPESFTTERIIPNAATAAAAATAAAAATKTKEIKAKMPPK
                     TRRLLLVALLALALLVLFFLLKPSTPETVEELQSQQGGSLPIEFRPVDEAEAKRAEEQ
                     ARAEQEAAEAAKAQEQAQAAQQESVTVAPAQTEQTMAKPSQDSQSQTQSQAVVEQPVA
                     VKPVENKPVVAQPAIKPKTENSVVFQPETTVKQQPKAPAKTEVKTERATLAAKETAKT
                     AAKPVEKAPTKTEAVKAAPAAESANAVATKTMTVPKGVSLMQVFRDNNLNISDVNAMS
                     KVNNVVSNLKVGERVTVRLDKNNRVVEMSIGSGGKFTRQANGSYTFK"
     gene            22124..22462
                     /locus_tag="PXE44_000322"
     CDS             22124..22462
                     /locus_tag="PXE44_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDL5587868.1"
                     /translation="MKKIKQLGLTFLFGLNAMAFAQTPPTTMPVEQNAVTQPVQQTQI
                     TNANAVNINTASAAELQDKLVGIGQKKAQAIVDYRTKNGNFKNIEQLAEVSGIGQATV
                     EKNRANIILE"
     gene            22627..23520
                     /gene="psd"
                     /locus_tag="PXE44_000323"
     CDS             22627..23520
                     /gene="psd"
                     /locus_tag="PXE44_000323"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251643.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL5587869.1"
                     /translation="MQLKKYPVPTYFQRVKIALHYLLPQLAITRAAGWLAEQKWGVVT
                     HLIIKLFAKQYKVNLSEAKKTEPSDYATFNEFFIRELKENARPINQDPNTLCLPAEGK
                     VSESGEISDNRLLQAKGHHFTLETLLANDEEMAAKFKDGTFITTYLSPTDYHRVHMPC
                     DGTLRKMIYVPGELFSVNPFLAEHVPNLFARNERVICEFDTAFGPMLQILVGATVTAS
                     MSTVWAGVINPPRADEVVVWNYETEGEKAIKLQKGEEMGAFRLGSTVINLFPKGKVHL
                     NPALIAGMQTRMGEELGRIIN"
     gene            23592..25943
                     /locus_tag="PXE44_000324"
     CDS             23592..25943
                     /locus_tag="PXE44_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5587870.1"
                     /translation="MYTLQELKQMNEQEIRSRLISPAIRNAGWSDRQIGEEYTFKTNK
                     RFTDGQVVVDPKTTQTKRIEAKRVDYLLYTSANQKIAVVEAKDNHHSSRHGLQQAMTY
                     ARLLDVPFAYSSNGDEFVEHDFITGVQRTLPMSAFPTPDELHQRWAKQYTTEELKIIQ
                     QPYYTSSTDTFKEPRYYQEIAINRTVEAVAKGQQRILLVMATGTGKTYTAFQIIHRLK
                     TSGAKKKILFLADRNVLVDQPMMQDFKPFKDKMVKVSNKKLDSAYEVYLALYQQLDGE
                     TDNELFKQFQPSFFDLIVIDECHRGSAKEDSDWRAILKYFKDATHIGMTATPKETDDA
                     SNITYFGEPIYTYSLKQGIEDGFLAPYRVIRVDLDCDLKGWRPTKGLKDHQGNEIEDR
                     LYTVKDFDYNIVLNDRTRLVAERICEFLRKTDRLQKTIVFCANINHAERMVAELSRLN
                     ADMLKHNSKYVCQITGDQSGKGVDLEEFCDVANEGKDYPVIAVTSKLMTTGVDSKTCK
                     LVVLDANIQSMTEFKQIIGRGTRLRPDFGKEFFTILDFRGVTRLFADPAFDGEPVVIL
                     DVPENKPLVEQPLPKPSDKDIIKDPELKPYKIPVVVDFGTLKLRILDEKVQYLNEQGE
                     LVSEDIDLFSKREMQKEFGSYEQFQQQWTTSGEIFSKFYTDPKWLAALRQTRQFSHDV
                     EDFDVLSHISFGKKPLTKTERAEKVKQSGYVEQYSPENQQVLGLLLNEYVKSGNQDLT
                     KTEILKTPQFSRFGGLVSIIKNFGGKELYQKAVKGLEKMIYSE"
     gene            25991..27460
                     /locus_tag="PXE44_000325"
     CDS             25991..27460
                     /locus_tag="PXE44_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL5587871.1"
                     /translation="MSLNNIVKRLQDVMRNDAGINGDAQRIEQIVWILFLKIYDAKEQ
                     EWELENDEYHSILPNFLRWQNWAEDKKDGKAMTGDELLSFVNNELFPTLKNLPISADT
                     PMNQRIIRAAFEDNNNYMKNGVLLRQVINIIDEIDFAHYQERHAFGDIYENILKSLQS
                     AGNAGEFYTPRAVTDFMVQAIKPKLGERIADFACGTGGFLTSALKVLESQIQTLSDRT
                     LFNNSVYGIEKKALPHLLCITNLLLHDIDNPNVHHDNALEKSVKDYTENDKFDVILMN
                     PPYGGSEIEQIKKNFPTALQSSETADLFMSVIMYRLKQNGRAAVVLPDGFLFGTDNAK
                     VAIKKKLLEEFNLHTIIRLPHSVFAPYTSITTNILFFDKTEPTRETWVYRLDMPQGYK
                     NFSKTKPMKLEHFNEVVEWWGNRQVIEVEGFDKAKSFTLKEMSDRQYNLDLCGFPHEE
                     EEILPPDELIADYQRKRTAYNADIDKILAEITDILGIKL"
     gene            27473..29131
                     /locus_tag="PXE44_000326"
     CDS             27473..29131
                     /locus_tag="PXE44_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL5587872.1"
                     /translation="MLTWEHIEKMIAHQQFIREKAEIEYKEAKNSVPKDWHTYSAFAN
                     SNGGFILLGIKENEQGHFPELLISGVNDPDKVIDELFSQSRSEKVSKSLLKDNDVKIF
                     QVENKSVIAIYVKAADILDRPIHLNGDYRLSYVRLNSGDHKLTSSELRSFISSYSQKD
                     ADGRIVPYASIDDISLSTLDKYRNLLKAHNSTSKLLTLSDEKLLQEIGAYKKDLAQNS
                     EGITNAGLLMFGKHYAITSIFPHFFFEYYEEAGDVKRYTLRITDFDLEEGNLFEFYLK
                     IVPLIQELGKDKHFKLDNLTRTEENEITDGLREALINAITHADYFNNVRHLKVIKTKD
                     NKLIFENAGVMLVDIPTAIAGQRSECRNSIIHNLFHRLGLCERQGQGVRDIYSYWQSR
                     NFNSPLLESEIDYTKLTLTFQSGMIADFEEKFTACFGENFSELQPIQKDCLLFIAFNQ
                     GKAMFAELAQKLPQYPRRNITLALARLKEKGLLTSSGSPRKQLYTLVNLEWIITEKSQ
                     DDYFNPPFDQLDKASESKGAPPKKSKVKQVKQLPQNKANKLPPK"
     gene            29337..30857
                     /locus_tag="PXE44_000327"
     CDS             29337..30857
                     /locus_tag="PXE44_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5587873.1"
                     /translation="MKAQQLKNAILQLAIQGKLVPQDPNDEPASELLCKIQAEKDRLI
                     AEGKIKKNKKTADKAPYTIEPPFEIPESWVWVRLGDICLKITDGTHHSPPNIDKSDFL
                     YITAKNIKKDGLDLSKISYVTKEIHNEIFSRCNPEKGDILYIKDGATTGVSIINTLNE
                     PFSMLSSVALIKTSQEIDNEYLNYVMNSHYFYNISIGSMSGTGIPRITLTKLESYLVP
                     VPPLLEQQRIVQKIEELLPLVERYEQTEQQLTKLNNTFPEQLKKSVLHAAIQGKLTEQ
                     DPNDELASCLIERIKAEKNRLIAEKKLKKPKSVSEIVMRDNLPYEIKAGQERCIADEV
                     PFEIPQNWTWVRLGEVGDWSSGATPNRHNPEYYNGTIPWLKTGDLNNGIISSIPEFIT
                     DKALQECSVRLNPVGSVLIAMYGATIGKLGILKIAATTNQACCACIPFNGIYNKFLFY
                     YLMSQKAEFQKKSEGSGQPNISKEKIINYLFPLPPIHEQHRIVQKIEQLFAEIEKL"
BASE COUNT         9462 a         6535 c         5936 g         9130 t
ORIGIN      
        1 taattatgaa aatttatact tgggacgaaa ctaaacgcaa aatcaattta gccaagcacg
       61 gattagattt tgccgatgca gatgaagttc tctcatcgcc ctatcgattt gatgttgaag
      121 ttattcgtaa caatgaattt agaatacaat cttttgcata ttcatttgga cgtttactcg
      181 tattatcact tgcatataca gaaagggaag attacatcag agtaattagc tttcggcgag
      241 catctaaagc agaaagagag gcatattatg aatacttaca acaatacttt gaataataaa
      301 aatttagaga aaaaacagct acttatggct attcaagcta ttccggcaag ccaagatttt
      361 atttgggacg gaaaagatga cgatgaacgc ccggttacgc catcagagtt ttcaattgca
      421 atgaaaaaac gtggcagacc agctggttca aagaaagaat tggtttcgtt acgtcttgat
      481 aaaagcgttc ttgaagcctt tagagcttca ggtaaaggtt ggcaaagtag aattaatcaa
      541 gttttaagtg attttgtaaa acaagtttga ccacaaaaca aaggcggtta atccgccttt
      601 ttcccttcaa tatatcgctc gaagttttgg atatattcat ctaaatttgc agtgagcatt
      661 tctttgtatt gctccacaaa atattgaata acttcttctc tggaatcggc caactgctgt
      721 ttatattccg ccttgcttgc cgcaacatag gcgttaaatc tggcacaacc aaacaacatc
      781 gcattgctga cttggtttgg ggcaatgttt tttaaatgag cgttggcaac gttaataaag
      841 ccgtctgctc gctggtagac gctggtagaa agttgggtca atttgtttat cggtaatgtt
      901 aatcatagta tcctcatttt tagtcaaaat acaagcggtg ctttttgcgt aaaattttgc
      961 acaaacgctt gcgtttgaac gttggctctg ccgacggcac gaagtgcgag cgtagcgagt
     1021 aaaaaaatca ccgcttgtag gcatttctag ccgtctaatg aaatcgcttc cttgattttc
     1081 ttcaatgcgg cattttcaag ctgtcttacc cgctctgccg agatgttata tttatcggct
     1141 aaatcttgca gtgtggcttt attgtcatct aaccagcggg ttttgatgat gtcttggcta
     1201 cgttcatcaa gggtggctaa cgcataggca agttgagcgg ttgcctgccc attatgttgt
     1261 tcgtcttcca agtcatcggc gaaattggag ctgctatctt caatatacat agaaggagcg
     1321 tagctgtctt catcgtcatc accggcaggc aggtcaaaac ctaaatcttg tccggtcatt
     1381 cgagattcca tttctcgtac ttcttccact gaaaccccta aatcatcggc aacttttttg
     1441 atctcttctt cattaaacca cgccaagcgg tttttatttt tgcgtaaatt aaagaataat
     1501 ttacgttgag ctttggtggt tgccactttc acaattcgcc agtttttcag cacatattcg
     1561 tgaatttccg ctttcaccca atgaaccgca aaagaaacca accgcacgcc gacattcggg
     1621 tcaaaccgtt ttaccgcttt catcaagccg atattaccct cttggatcag atctgccagc
     1681 ggtaaaccat agcccaaata gccacgagca atatggatca caaaccgcat atgggaaagg
     1741 atcagctgtt ttgccgcctc aacgtcttca tcataataat aacgctccgc cagttctttt
     1801 tcttgctcgg cggtgagaat cggatactga tttgccattc ggatgtagct atccaaattg
     1861 ccctgcggca caggcattga gccggaaacc aaagccggat gcattgcttc taccgatgtt
     1921 tcttcttcgt ctatctcatc aacatcaatc ggctcggcat ctataatttc agcatcttca
     1981 atctcgttct cgttaaattt tttcattcta tttctcttca ctctcagcaa ggtttacatt
     2041 ataacagagt taaatctatc tggtgtggga tttgtatgat tttatttcga gctattcaat
     2101 accgctaagt tccgccactt gcaaaacttt ttgcaaattc gaccgctttc ctttagaata
     2161 ccgctacttt tggggctgat tttggattcg acgggattgg cgaagcccaa ggtgcatgtc
     2221 gaggtgcggt aggcctcgta aacaaaccgc aaaataatag tcgcaaacga cgaacaatac
     2281 gctttagcag cttaataacc tgctttaaag ccttatctcc tcagcttccg ctcgtaagac
     2341 gagggcaaag ataagtcacc caaaacgaga tcgtgtggac gccgccgctt taggatcgaa
     2401 acactaaatt aaataaagct agtttattca ttgcgtgtct gtccgctgtg aataagcgaa
     2461 attaaagact agactaaaca tgtagtactg aaggtagaga aatttcggac gggggttcaa
     2521 atccccccag ctccaccaaa ttataatcct atacggttca accgatacct aaaaagcctt
     2581 gaaaatcaat gtttcaaggc ttttttattg tccgttgcgt acctatacga tacaatcaaa
     2641 tccgaagatt ttaggagtat atttaggagt acgccggaat acccccaaaa attcgtactc
     2701 ctaaaaccac tttagagcct tttcaaataa ggctttatcg cttttagttt tgtactccca
     2761 aacaaaaaat catactccta agatttaggc tttatactcc taaaggttta ctatggctag
     2821 aaaaatcaca ccgttaaacg atacccaaat aagaaaagcg aaaccggaag acagcccatt
     2881 aagagacggt aacggcttac tacttgttat tacctcaaat tctaaattgt ggcggtttag
     2941 gtatgaaaga ccgttcacaa aaaagcggaa tgatttaagc attggggctt accctgatat
     3001 ttctttagca caagcgagag aaattagaga agaatatagg gcattattgg ctcaaaatat
     3061 cgaccctcaa acccaccgcc aacaacagga acaggcaaag gccaacgaaa tcagcaacac
     3121 ttatgaaaat gtagcgtggg cgtggctgga acaccgcaag accaagaaga atttttcaga
     3181 caactaccaa aaagatgtaa tcgccttaat taaaaataac ctcttgccgc actttggaag
     3241 aatgcctatc acacaaataa ccgcaccaat ggcaatcaag gcattcaagc aataccaaga
     3301 cgagggcaaa ctagagaagt taaaacgggc aattcaaaaa cataatgaaa ttatgatgtt
     3361 tgccgtacac cgtgaaatga taccgtttaa ccctaccgcc aatattgcga aagagtttga
     3421 tagcccaacg gttgaacatt tcaaaaccat taagccggaa gatttaggcg agtttttata
     3481 caccttacaa aacgcccaaa ttcatttaca gacacgctat ttaattctgt ggcagttact
     3541 cacaatgacc cgcccgaatg aagcggctaa ggcaaaatgg gcggatatag acgaaaaagc
     3601 caaactgtgg acgataccgg cggaaaatat gaagcgaggc attgagcata aaatcacact
     3661 ctcacgccaa gcaatcgcac ttttgcgaga gattaaaaag ctaagtggcg gtaaaacgta
     3721 tttattccct agcgtgaaga acccacaaac gcacgtcaat actcaaaccg ccaacggtgc
     3781 gatcaaacga atgggctaca agggcaaatt agtggctcac ggcttgcggg ccatcgcctc
     3841 cacttatctt aatgagaaag gctacaatag cgatttaatc gaggttgcct tatcgcattt
     3901 aaacagcgac cgaatcaaag ccgcctatga tagaggcgaa cgcctagagc aacgttttaa
     3961 actgctccaa gtgtgggcgg attttgtaga ggaatgttcg caaggtagct taccgcaata
     4021 tcatttgaaa atagcctagt aaattttccg ccaaaaatga ccgcttgtaa tgccgttagg
     4081 taaaccgcct gacggctttt ttatgcccgt aattcccctt gatgaaaatt ctgtaaactt
     4141 ttcgattttt acccacttta cccacttttc actattattt ttttataact aactgaaaaa
     4201 taaagaaaaa atgacagtgg gtaaagtggg ttaaaagtgg gtaaatggca aaaacccttg
     4261 ctcactgtat cttaatggat acaataagca ataaatagaa taagaagccg gcaatgatga
     4321 gagagcaaac cactaatcaa attattatta ccgataattt ttcaaaatgg cttgagaatt
     4381 taaagaaccc tatcgcaaaa gcaacggtag caagacggat aaaaaatgcg attaatggca
     4441 actttggcga tcataaatct ttagccggta caggcggact ttatgaaatg cggatagcaa
     4501 caggagccgg atatagaatt tattacgcat aacaaggaaa tgtaatttat atcttgttac
     4561 acggcggcga taaatcaaca cagcaagccg atatagagaa agcgaaagca ttatgggctg
     4621 aattaaaaac tacaggagaa agcaatggaa acaatacaaa tcaatgaaca ggcagcagaa
     4681 aagcacggtt tacgcccttt tgacgtggcg gagtttttaa ccgatgaggc aactattcaa
     4741 gcctatttag cggaagtgtt aaaagagggc gaccaagacg aatttttaga agccttgaac
     4801 gatgtagcaa gagcaagagg aatggcggag ctggccaagc aaacagggct aggcagagag
     4861 agcctatata aaaccctagc caaaggcagc aaaccacgct ttgacactat tcaaaaaatt
     4921 ctaggggcat tcaatttagt gcttattcca caagtcaaaa atggctaaaa cttatcactt
     4981 gctacctgtc aaaacctcac ttttaaatag ccgttgatgg atattgacgg ctttttattt
     5041 gctccttgcg tgcgtactgc tccgaaaatt ccgcactatt cgcaaaacct taatgaaata
     5101 tgaccgcttg aatgctcttt tttatagcgt ccggaaaccc atctttagcc tattttgatt
     5161 tactgtaata aaacacagct tattacttgt aatttatctg aataattagc aaaatacaac
     5221 ctatcaagtt caatccgtaa ctatacggat ttattcaaat tagcgtggat agtggcggag
     5281 taattaaccg gcacgaaagg agaaagccct ttatttctct ttccacgctc tcacagtaaa
     5341 gggatacgtt aaaggggcgc ttatggcaga tcacagtcaa tttatttcta ggcttgaaaa
     5401 tcgtattgag cccaaaatca gctttcaaga actgatacaa gaattatcaa actatacaaa
     5461 taaacctttt tcacatatag cggataaatt gaaaagcctc attttttcct ttcagcaaaa
     5521 tggaatatat acgaatgctt ttgtaaattg cactctcttt gaagatggtg aaagctatgc
     5581 tattgagccc aaaacactac atttcaaaga cgctttcgat cagcctaaag gcgttttaag
     5641 tgatgttatt gctcaaaatt ctattgatgt tgaacattta aatgcttatt acgtaacagc
     5701 aagagaaata acacagctga ttaaaacaca atcgccaacc ttgttagata aattaaccac
     5761 caagcaaaca caatcagaga ttagccaaat tcaaaatgat tatatttctg tttatgactt
     5821 catagaatgg gcaagtaaac attactcaag tcttagtgaa acagctaacg atttaaaccg
     5881 cttactcaaa gataaaaata tacagcttta tcgacaatat ggcggaataa gtccaagcat
     5941 tgataaagat aatactaatt taaaagcagc ctttgatttt gtagcaatta ataacggata
     6001 tgaaaaacaa tcaagcagct ttaattttga tgatgatatt ccgttctagg tggcagcaat
     6061 gaatttaaat gatttccaaa atttactttt aaataaacaa gaattagaag cagcactagg
     6121 gaaaaagctc gatttttctg agataacacc tgattattta gcagactatt cacgttttta
     6181 agcctctatt ctgaaaccga gcttacccat tgcccgagct tttaattgtg ctaggcaacc
     6241 gaatagcgaa gctggaacaa caacataacg caatgccaag cgaaaccgat tcaatcacac
     6301 tcggcatttt aagaaaacaa cttagccaac tgttaaaagt ggcggaaaga ataaaggaat
     6361 aaaccaaaat gaaacacgag cttaatttag cgggtattac tgctttaatg aatgaaccta
     6421 tcaaagcaac cttgagcttt ttagattttc cgcatatccg aattgaacga ctgcaccacg
     6481 ataacggcag accgtttttt atcggtgtgc tttctcttga ccttgaagat catatcaata
     6541 ttgaaatggc aggggctacg ggctttttag aggctctagc gattattgcc tatcatcaac
     6601 agctaaccgc cacgccgcta ttagtgaatg atgacttatt gagtgaaatc aacaagctcc
     6661 cccacccaca acgaacaaac ccatttattg accgacacgg caacgaaatt acacgggagc
     6721 agatgagcaa agaataccga gaaaaaatcg attatgcctt tgctgaaaag cgaatggcgg
     6781 aggggaaaga atgaccgaac tcacaccaaa acaagaggct ttttgctttg cttatattga
     6841 aaccggcaat gctagcgaag cctaccgcca agcctatgaa accgaagata tgaagtccga
     6901 aaccgtccac cgaaaagcta aagagctaat ggataacggc aagattacgg caaggattga
     6961 ggaactgaaa gccgaacacg cagaacgcca taaattaacc gttgatgacc ttttaaagga
     7021 gctggaagag gctcgtatac tggcgagaga gaaagaaaac cctaacgcaa tgactcaagc
     7081 cacaatgggc aaagcgaagt tgctagggct ggataagcag gttaccgatc cgtgctttga
     7141 tggtgaaacc ggtaaaggag atatgatttt aagtgtaaac tgctcaagca ttaaagacgg
     7201 gaaagcactt gatgaaactt gtattttaaa ggcaggaaca cacgaattta tcacacggga
     7261 cagctttatt ttctaccgcc atataagaat cgattcgtta gatgagatta aggcaaatat
     7321 agaagccggt tatttcattc aaaaagccct aattaatgat gaagcctatc aacaaatact
     7381 aaaaggcgta ctatcgtcaa aatcagtaac gcctagatat aaacgcttcc taaaaaatgc
     7441 aatccgccaa agtgcttgcc ctgatattct agccgaacct taagccgttg atgagaatta
     7501 aggccttttt tgccctttaa ccactaccaa aaccgctaat atttcccctc taaaagtggc
     7561 taacagtggg taaaaatcaa agttacccac ttaacccact gtaacccact tgcttcccac
     7621 tggctataaa gccttatata tcaaggcttc caacaaatca gtgggtaaag tgggtaaaat
     7681 ttcaaaaagt tttaaatttt ttctttataa acttaaacta ctatagaaaa ctacgaacta
     7741 aatttcccta tacacgatcc gaataattgt acacaaaata gcctaagcaa taaggcactt
     7801 tgaattttag gagtatattt aggagtacaa ataaaatttg aattaaccaa aacccttatt
     7861 tatcaatgct tgaagtgata caattcagat gaccccagca ccaaattata atcctatacg
     7921 gttcaaccga tacctaaaaa gccttgaaaa tcaatgtttc aaggcttttt tagtaacatt
     7981 atgtttgaac cgattcaagt gtagcttcag gttacataaa gcttaccact catatccaac
     8041 tttgagtgaa atgaattttt gttcttgtaa tgtatttcct tttgtaaagc cagcatgtac
     8101 cgaacttgat acccctttat agtggagtgt aatccctact tcttgcttaa aataacggcc
     8161 tatattttga tttaaggaaa attcatttaa cttagtttta agtgttccgt ggcttgcatc
     8221 aacaaaatag cttcctaatt caggttttac ctttaattca ttaaatataa aagttttatt
     8281 taatgctaaa ccagcttgaa tggccaaaag atctacccta tcagtctcaa attttgtgtt
     8341 ctctaattgg gcatttgcag atgttaaatg atgatagcgc ataccaaatg acggttgtag
     8401 gttaaaactt gcaacttcca acgttttacc taccgttaat cctaaagaat atacatttct
     8461 ggctagtttt atcgtctggc tttgatggct taatttgtta ttcgcacgcc caatgctacc
     8521 atcaatccct acataaatgc cattttccca tgttttctta ccataaaaag aaaacatagt
     8581 taaatcccct ttaccattat agtagttctg aaaatcatta aaggttaatt gcaacttgta
     8641 cttagcaatg tctaaatcat aaattaaagg aataactctt acctaaatcc catcaccaaa
     8701 caaaccttgc tcatcttcaa agtattgatt catatcaaaa gccggttttt gttcagagga
     8761 tttgccgata atgcgagccg gtacacctgc tgctgtggcg tgatcaggca caggttgtag
     8821 caccactgaa tttgccccga ttttagaata gcgtccgatt tcaatattac cgagaatttt
     8881 tgcaccggct ccaatcatca cgccttcacg gattttcggg tggcggtcgc cattttcttt
     8941 ccctgtaccg cctagtgtta caccttgtaa aatcgagaca tcattttcga tcacagaggt
     9001 ttcacccacc acaataccgg tggcgtggtc aaacataatt ccgcaaccga cctttgccgc
     9061 aggatgaata tccacatcaa aggcaacaga aatttcattt tgtaaataaa ccgctaatgc
     9121 tttcctgcct tgattccaaa gataatgcgt cacccgatag ctttgtaaag cgtggaagcc
     9181 ttttaagtac agcagaggtg tactccattt gtccaccgca ggatctctag ttctgaccgc
     9241 attaatatca caagctgcac tagcaataat ttgtggctct gctcgataag cttcctcaat
     9301 aatttctttt aaggcgattg ccggcataat cgggttttct aacttattcg ccaaaatata
     9361 acttaacgca tcgcctagat tatggtgttt taaaatggta gcgtggaaaa aacttgctaa
     9421 cataggttca caattcgcca gctctttggc ttcatctcga attgcttgcc aaatctgatt
     9481 aatttcactc tgctgcattt tattctcctt tctttattcg ccttttcgtt cacgccccaa
     9541 caaggttttc accacatccg aagcctgttt gccacaaaat aacatttggt aaatttgttc
     9601 tacaatcggc atttcaacgc cctgtgtttg agcgagcaaa taggcttctt tggtattata
     9661 aaatccttct accacttgac cgatttccgc tatcgcgact tctgccgatt ttccttgccc
     9721 taacgccaaa ccaaatcggc ggtttcggga ttggttatcg gtacacgtca gcactaaatc
     9781 tccaagcccc gccattccgg taaaggtttt cggatcagcc ccgagcgaaa cacccaaacg
     9841 gctaatctcc gccagccctc gagtaatcaa ggcggtacga gcattcgccc cgaatcccat
     9901 tccgtctgaa ataccggcac caattgcaat cacattttta atagccccac cgagttgcac
     9961 accgataata tcattgttga ggtaaactcg gaacgctttg gaacagtgac tccgtttttg
    10021 catttgatcg gcaaaaacag gatcagttga ggcaagcgaa atcgctgttg gcaagcctgc
    10081 cgccagttct ttggcaaaag tcggaccgga aagcaccgca agcgggtaaa tttcccccaa
    10141 aatttcctta gctaccgttt gcaacaaacg cccggtatct cgctccagcc ctttggttgc
    10201 ccacattata cggtgatctt gggacaaaaa aggcttgatt tgagccaata cttcagagaa
    10261 tacatgactt ggcaccacaa tcagcagatc tttcgatttt tgtaccgctg tagcaagatc
    10321 tgcgacaatt tctaaggctt ccgggaatac aatatcaggc agaaaatctt ggttcattcg
    10381 ctctgatgcc aaaacggcca ttttgtcggg attatgcccc cataaatagg ttttgtgacc
    10441 atttcgagaa agggcaatgg ctaaagctgt tccataagaa cctgcaccta ataccgtgat
    10501 tggggcagaa tagatttcac tcatatttcc tctgttcggc ttatttagaa aattgcaaaa
    10561 aatagtagaa aaaataaccg cttgtaaaaa aataggtatg ccttgcatac ctattttcta
    10621 tctaaaatgc ttagttcgct aaagccggct cttctgcact ttcttcttgg cgttggagat
    10681 aatccataaa taacgcatca aagtttaccg gtgagagatt taatgccggg aatgtaccac
    10741 gattcacaag gcttgagatt agctcacgag cataaggata aagcacattc ggacattgtg
    10801 aagctaagca gtgagcaagt tgaatgcctt ctaaaccttt aatagtaaac acacctgctt
    10861 gttttacttc acaaatgaaa gcgacatcat tgctgtcttc taacgtagtt tgcaccgtaa
    10921 tgtgtaaggt aacttcgtat aaatcttcac caatttcttt ggtttcagta tctaactcaa
    10981 agcctaattg tggtttccac tcttgttgga aaatagtcgg taaatttggt gcttcaaatg
    11041 aaacatcttt tacataaatg cgttgaattt gaagctcaaa aggggtttgt gtagcttctt
    11101 ctgttgatgc aacttgattt tcttctgaca taaaatttcc ttaataatag attatttatg
    11161 ttttttaata gttggtaggc ttgctgcacg ccagccgcca atcccttctt ttaatacata
    11221 aactctgcta aagccttgtt tagcaagatc tgccgcaaca ccacctgaag tgaagtcatt
    11281 agtgtctact acaattaccg ggcgagcttt gtatttatca atctgattga ttttagctgc
    11341 tttaatgtct gacggtaaca cgtgaatgct atcaataata tgaccggcac ggaactcttc
    11401 atcagaacgt aaatcaacca ctactgcctc ttctttatta atcagctgtg tagcctgtgc
    11461 gttatcgaca attttatatt tttgcgttgc acttttataa aagctaaaaa ttaccgcaat
    11521 caataatgca acccaagcaa acaccatcaa ggtatgattg gcaaaaaatt gctctaattg
    11581 ttgagcaaaa gttagttgtt ccataattca atttccttaa catcaaaaaa taagcggtcg
    11641 ttttgatgca aatttatcac taaatttaac cgcttgtaaa agtaataaaa ggtgagcaaa
    11701 agcccacctt ataaggaaat tattgagctt gttgtgacat tacttcgtca aatgacattg
    11761 ttttttcagt cactttcgct ttcgctagca ctgcatcaac cgcttgctct tctaatacca
    11821 cattgcggat attttcagtt aattgacggt ttttcgcata gtgagcaacc acttcagccg
    11881 gttgttcgta agctgaagcg atatctgcga tcatctcttc aacacgtttt tcatctactt
    11941 ttaattcgtt agaaccgatg attgaagata ataataagcc cacttgaaca cggcgtttag
    12001 cttctgcttc aaataattca gccggtaatt gagctgccat ttcaggctta ccaccaaaac
    12061 gttgcaccgc ttgttggcgt aatacgttga tttcttcttc aaccgcagca gccggcactt
    12121 caacatcatt ttgagcaatt aagccgttga ttacttgagt tttcacacgt gaagtgactg
    12181 catttttaag ttcacgttgc atatttttct taatttctgc gcgtaattct tccactgatt
    12241 ttgcattacc gaattttttc acaaactctt cggttaattc cggtaatacc atctcttcta
    12301 cttttttcaa ggtaattgca aatttcgctg ctttgccttt taagttttca gcgtggtatt
    12361 cttctgggaa agtcacatca atgtcgaatt gctcacccgc tttacgacct acgattccgt
    12421 cttcaaaccc cgggatcata cgaccttgcc ccataaataa cacgaagtct gatgctttac
    12481 cgccttcgaa ttcttcacca tctactgaac caacgaaatc aatagttaca cgagattcgg
    12541 cttttgcagc ctcttggctt tctgaccaag tcgcttgttg tttgcgtaat acatctacca
    12601 ttttgtctaa atcagcgtct gcgatttcaa caaccggttt ttctacttcg atattctcta
    12661 aaccttttaa ttccacttct gggaatactt caaattccgc agtaaagctg aagtcttgac
    12721 ctggttggta gttgtttggc gtgaaagtag ggcgacctgc caagttgatt ttttcagcaa
    12781 tgacggcttc aaagaatgca cgttgcattt catcagataa cacatcttga cgggctgcaa
    12841 taccaaaacg ttgttcgata attgagtgtg gcactttacc tttacggaag ccatctacac
    12901 gagcattttt cgcatagcct tttaattgtt ctttgtatgc tgcttcgatt ttatcagctg
    12961 gtacgttaat agtaacacgg cgttttaagc cttctaatgt ttcaattgaa aatgacattc
    13021 ttaaacctcg gtttagggat ataaaaaaga tatacaaata gacggttatt gtagcgttct
    13081 atcaagaaac tgtcgagaag aaactcattc gcaaataaac agttttgtaa aaaatcccta
    13141 aaatctgact gcttgtcaat gggcttgcct acaagcggtc ttttttcacc gatttttgca
    13201 aatgaaatga tatgatatgg ctagcttaat cggcataaaa gcaaaagagg catacctttc
    13261 gatatgcctc tttctagttt ttatttagct tttcaattta tcaaacaatc ttaattattt
    13321 gatgatttta gctacaacgc ccgcacctac tgtacgacca ccttcacgaa tcgcaaaacg
    13381 taaaccttcg tccatcgcga ttgggtgaat taggctaaca gtcattttga tgttgtcgcc
    13441 cggcattacc atctctacac cttccggtaa ttcgattgta cctgttacgt ccgttgtacg
    13501 gaagtagaac tgtggacggt aacctttgaa gaatggagtg tgacgaccac cttcttcttt
    13561 tgataatacg taaacttctg attcgaagtc tgtgtgtgga gtgattgaac ccggtttcgc
    13621 taatacttga ccacgttcga tttcttcacg tttagtacca cgtaataatg cacctacgtt
    13681 ctcacccgca cgaccttcgt ctaataattt acggaacatc tcaacacctg ttacggttgt
    13741 tttcgctgta tctttgatac ctacgatttc aacttcatca ccagtgcgga tgataccacg
    13801 ctcaacacga cctgttacta ctgtaccacg acctgaaatt gagaatacgt cttcgattgg
    13861 taataagaat ggtttatcaa tcgcacgctc aggctctgga atgtatgtgt ctaagtggtt
    13921 tgctaattca aggattttct cttcccactc tgctacgccg tttaacgctt gtaacgctga
    13981 accacgtacg attggagtgt catcacctgg gaagtcgtat tgtgaaagaa gttcacgcac
    14041 ttccatctca actaattcta ataactcttc gtcatctacc atgtcgcatt tgtttaagaa
    14101 tacgatgatg tatggtacac ctacttggcg acctaataag atgtgctcac gagtttgtgg
    14161 cattggaccg tcagttgctg ctactactaa gatagcgccg tccatttgtg ccgcaccggt
    14221 aatcatgttt ttaacatagt ccgcgtgtcc cgggcagtca acgtgtgcgt agtgacgagt
    14281 tgctgtatcg tactcaacgt gtgaagtgtt gatggtgata ccacgcgctt tttcttctgg
    14341 cgcgttatcg atttggtcaa atgcacgagc tgcaccaccg aagtgttttg ctaatacggt
    14401 agtgattgct gctgttaaag ttgttttacc atggtcaacg tggccgattg tacccacatt
    14461 aacgtgcggt tttgtacgtt caaatttttc tttagacact ttaatatctt cctaaaaaat
    14521 gagccatagc cctattgcta tggctcaggt taaacaaatt attttctacg agcttcaata
    14581 attgcatcag caacgttttt cggtgcttca gcatatttta aaggttccat agagtatgat
    14641 gcacgacctt gagtttgtga acgtaagtct gttgcataac caaacatttc tgaaagtgga
    14701 acttcagcat tgatcttaac aacgaactcg ttcgcttctt gaccgttaac cattgcacga
    14761 cgacggctta agtcaccgat tacatcaccc acgtaatccg gtggagtttc aacttccact
    14821 ttcatgattg gctcaagtaa taccgggctt gcttttgcga acgccgcttt aaatgctaat
    14881 gatgcagcaa gtttaaacgc aagttcagat gagtcaacat cgtggtatga accgaagtgt
    14941 aaacgcacac ctaaatctac taccgggtaa cctgctaacg gaccagattt aagctgttct
    15001 tggatacctt tatcaaccgc aggaatgtat tcaccaggga ttacaccacc tttgatctcg
    15061 tttacaaact cataacccgg accttcagca tctaatgggt ataagtcgat cacaacgtga
    15121 ccatattgac cacgaccacc agattgtttt gcgtgtttac cttcaacatc attaacacga
    15181 gtacggatag tttcacggta agatacttgt ggtttaccga tatttgcttc cactttaaac
    15241 tcacgtttca tacggtcaac gatgatgtct aagtgtaact cacccatacc tgaaatgatc
    15301 gtttcaccag attcttcatc tgtgtgaaca cggaatgaag ggtcttcttg tgctaaacga
    15361 cctaaagcta agcccatttt ctcttggtca gctttggttt ttggttcaac cgcaacagag
    15421 attaccggct cagggaattc catacgctca agaatgattg gtgcatcgat tgcacataat
    15481 gtgtcacctg taccaacatc ttttaaaccg attgctgcag cgatatcgcc cgcacgaact
    15541 tctttgatct cttcacgttt gtttgcgtgc atctgtacga tacgaccaaa acgttcacgt
    15601 ttttgtttta ctgagttgta aactgtatca ccagagttaa ttacaccaga gtaaacacgg
    15661 aagaaggtta agttacctac gaatgggtct gttgcaattt tgaatgctaa tgaagcaaat
    15721 ggctcatcgt cgcttgcgtg acgctcacct tcagtttcat cttcattgat acctttaatt
    15781 gcaggaatat cagttggtgc cggtaagtaa tcgattaccg catcaagcat tgcttgtaca
    15841 cctttgtttt taaatgcaga accacagcaa acagggatta cttcacctgc taatacacgt
    15901 tgacgtaatg ctgctttgat ttcatcttca gttaattctt caccagagaa gaatttttcc
    15961 attaattctt cagaagcttc agctgctgct tcaacaagca ttgcacggtg ttcttcacaa
    16021 gcttcaagca tatctgccgg aatatcttca taagtgaagg tcataccttg atcagcttcg
    16081 ttccagttga ttgctttcat tttgattaaa tcaacaacac ctttgaagct gtcttctgca
    16141 ccgattggaa gttgtaatgc aactgagtta ccacctaaac gcgttttgat ttgctcaaca
    16201 acacgtaaga agtttgcacc tgtacggtcc attttgttta cgaacgcgat acgtggcact
    16261 ttatatttgt tagcttgacg ccatacagtt tcagattgtg gttgaacacc accaactgca
    16321 caataaacca tcaccgcacc atcaagtaca cgcattgaac gttctacttc gatagtaaag
    16381 tcaacgtgtc ccggagtatc aataacgttg atacggtgtt gtggatattg ttgtgacata
    16441 ccagaccaga atgctgttgt tgcagcagat gtgatcgtga taccacgctc ttgttcttgt
    16501 tccatccagt ccattgttgc agcaccatcg tgaacttcac cgattttgtg acttacacct
    16561 gtatagaaca agatacgctc tgtagtagtt gttttacccg catcgatgtg cgcactgata
    16621 ccgatgttac ggtaaagtga aataggggtt gtacgagcca tttttattta ttaccttgtt
    16681 tggggtttaa attaaaattc ttaataaggg taaggcttca ccgcaaactg agatgaagcc
    16741 tttgataagt ttgcttaaac gattaccaac ggaagtgagc aaacgctttg ttagcttcag
    16801 ccatacggtg aacgtcttca cgtttcttca ctgctgaacc tttgttgtct gctgcatctg
    16861 ataattcgtt agctaaacgt aatgccattg atttgtcacc acgtttacga gcggcttcta
    16921 cgatccaacg cattgctaaa gcattacgac gggttgggcg aacttctaca ggaacttggt
    16981 aagtagaacc acccacacgg cgagatttaa cctcaacagt tggacgtacg ttttctaatg
    17041 cgatttcgaa agcttctaac gcttctttac cagaacgttg tgttaaagtt tctaatgcac
    17101 cgtaaacgat tttttctgca gtagattttt taccatctac cattaacacg ttaataaatt
    17161 ttgcaagtaa ttctgaaccg aacttcggat ctggaagaat tttgcgtggt tcaatactac
    17221 gacgacgtgg cattggaaat ttctccgtat attaaatctt caggatatcc aaaactcatc
    17281 taatctgcct taaggcatct tgactggagt ttatggttta aatattaaaa tttagacgtt
    17341 tggccttact caacggagaa ccattaagct ttaggacgtt taacgccgta tttagaacga
    17401 ccttgtttac gatctttaac gcctgcacag tcaagtgcac cacgtacagt atggtaacga
    17461 acacccggta agtctttaac acgaccacca cggattaata caacactgtg ttcttgaagg
    17521 ttgtgacctt caccaccgat gtatgaagtt acttcaaagc catttgttaa acgaatacga
    17581 catactttac gtaatgctga gttcggtttt ttaggtgtag tagtgtatac acgagtacac
    17641 acgccacgtt tctgcgggca agcctctaat gcaggaacgt tactttttac aacctttttc
    17701 acacgcggtt tgcgtactag ctggttgata gttgccatta aaaaagctcc agttaaaatt
    17761 ataaaaataa aattaaaaac atcctttcac acgaaaggac ggtgaattct aaattgaacg
    17821 tggataaaag tcaagcacag taagagaaca acaatgatca tttgttaatc aatggaagta
    17881 atttttctag cacttgtgaa gaggtaatat tttccatttt atctgctttt agataaaact
    17941 gattttgccc gtaagccccg ataagttttg gatcggttgc cccatataaa atcacatttg
    18001 gcttatctaa cgcagccgcc aaatggctca agccggtatc caccgacacc actgccgctg
    18061 ctcccgcaag ttgagtagca atatcggtta agctcatttt tggcaatacc ttagcattat
    18121 tcgccacact cgctaaacgc tccgcccgtt ctttttcggc ttggtttccc caaggcaatc
    18181 tgactgccag attgtgctta tccaacgctt gaatcaactc cgcccaatag gcttcttgcc
    18241 aatgtttatc cgctcgggta gtggcgtgaa tcagcataat gtaagcggtc gttttttgag
    18301 gattttttgc aaaatgttgt gcaatgcaat aatcgccccg ttcggttggc aaagcataac
    18361 ctaatgattt tgcacataat tttctgatgc gttctacagc gtgttgttgg taggggatat
    18421 taaacgtttc atcgtaaaac agactactta acccttctcg ggcagagttt ttatcatagc
    18481 caaatttttt gcctttggct tggcgagtta ctaacaccgc acttttaatc agcccttgag
    18541 catcaattac cgcatcatac tgctcacttt ttagctgttt taaaaactgc tgccattcta
    18601 cccaagtctg tctttggcaa aggtttttcc gccagcggcg aatagccacc ggaattactt
    18661 tatttaccgc tgaatgccac gcaggaattt cggtaaaatt ctcctccacc acccaatcta
    18721 cttttaaatc gggaatagca ttttgcatat cggttatggc tggaagcgta tgtaatacat
    18781 ctcccattga tgaggtttta acgattaaaa ttttcatctt ttataaacaa acaagcggtc
    18841 tttttttgca aaaaattggc aaattcaacc gcttgttatc catttaaact aacagttcag
    18901 ctaatttgtc catcaccatt tcaggctgaa tatcaattaa actttggtga tagccctctg
    18961 ccccttcgcc tttgcggatt ttaatcaaac cgccttcaat taagcgcatg atctcggcat
    19021 tttgggataa cggcggcgta tattgtgggc tggtcggtcc atataaggcg accagcggtt
    19081 tatttagtgc tgcggcaatg tgcattaagc cggagtcatt gctcacgact gcattacaat
    19141 ctgcaatcaa atctaccgct tggtttaaat cggtttgccc ggctaaattc aggcaatagc
    19201 gttgtaaatt ttcaggtaat gccaagcgaa tttgctcacc ggcggcttca tcttttttcg
    19261 agccaaataa ccgtaccgaa tagccttgct caatcaacat ttttgccaat attgcataat
    19321 gataatgcgg ccaacgtttt gccggtccaa attcagcccc tggacaaaag ccgatggcag
    19381 gacggttttc cgcataagcc aactgttttt caaatttcgc tttggtctgc tcaatttgtt
    19441 cgctatcagt tgtgaggtat ggataagcaa tcggcaactg ttgtgcggtt ggaacagtag
    19501 ccttttcaaa cgccaaggct acatagcgtt gcaccatcat tgggtagtcg tttttattac
    19561 tgcgaagatc atttaagaaa aaatatctac tttccccttt ccaaccacga cgtctcgcaa
    19621 ttttcgcaaa cagtggaata aaggcagact ttaatgaatt aggcaataca atcgccatat
    19681 catattgatt tctcagggat tttccgagtc ggtagcgttc acacaaacga aatgaaccgt
    19741 gtccaatcgg cattgaaatc gcattacgca cctcaggcat acgggctaaa agcggacaac
    19801 accaatcggg tgccattaca tcaatttggc aattcggata ctgtatttta agctgttgat
    19861 aaagtgcgtg cgacatcatc atatcaccca cccaagacgg tcctataact aaaatattca
    19921 tttgcaaaat tctcttaaaa atttattcac aatccttgta aggattgttc acagcttcgc
    19981 tgccgttggc aaagccaacg ttcaaaaagc gatacttttt gtgtccgctt gttactgctt
    20041 tatgcaattt gccgctaacg tttttttagt gttgttatac aaaaatgctt taccactacg
    20101 ggtttcatgc caagtccaac ggatattagt gtatttttta ccatctctcg ttacagaagg
    20161 tgaaagctga tgcgttgtcc cttgataact cactgtgact atttcctttt ttaacgcttt
    20221 ttttgttcgt ccctggcgtt caacttcaac cttcttatca ttctcacaac gatataaact
    20281 cactttaggt gcttgtgcta tcagtttagt cactttcttt actgactctt tttccttttg
    20341 tacttgatga atttgctcaa caggggtaac cactgtacta caagcaacta acatcgctga
    20401 gcttagcgtt aagtagataa gtttgtaaga ggggcaaaat aaattcatcc tttatctcct
    20461 ttttaaataa aagagcaacg tttgtagcgt tgctcttttt cttcatttta tttatttaaa
    20521 agtatagcta ccgtttgctt gacgggtaaa tttgccgcct gagccaatgc tcatttcaac
    20581 cacacggtta tttttatcta aacgaacggt tactcgctcg cctactttta aattactcac
    20641 cacgttattg actttgctca tcgcattaac gtctgagata tttaaattgt tatcacggaa
    20701 cacttgcatt agtgatacac cttttggcac agtcattgtt ttggttgcta ccgcatttgc
    20761 cgactcggca gccggtgctg cttttaccgc ttcagttttt gtcggagctt tctcaactgg
    20821 tttagctgct gtttttgccg tttcttttgc tgccagtgta gcacgttcgg ttttcacttc
    20881 agttttagca ggggctttag gctgttgttt taccgttgtt tcaggttgga acaccacgct
    20941 attttccgtt ttaggcttaa tcgccggttg tgcaacaacc ggtttatttt ccaccggttt
    21001 caccgcaaca ggttgctcaa ctactgcttg tgattgtgtt tgcgactggc tatcttgtga
    21061 cggtttagcc attgtttgct cggtttgggc tggagcaact gttacagact cttgttgagc
    21121 ggcttgagct tgttcttgag cttttgcagc ttcagcggct tcttgttctg cacgagcttg
    21181 ttcttccgca cgtttcgctt cagcttcatc aaccggtcgg aattcaatcg gcaagctacc
    21241 accttgctga gattgtagct cttcgacagt ttccggcgta cttggtttta acaagaaaaa
    21301 cagtaccaat aatgctaacg ccaacaatgc cactaataat aaacggcggg ttttaggtgg
    21361 cattttagct ttaatctctt tggttttagt ggctgcagct gccgcagtgg ctgctgcggc
    21421 agctgttgcc gcatttggaa taatacgctc agtagtgaaa ctctcgggtg caggtttaac
    21481 cacttctttt tccacatttt cttgtgcttc aaccgcgtta aaagatgcgg ttgatacact
    21541 ttcttcaacc gaatgaatgg tattatccgg ctctactgct tctgtagctt ctgaacttgg
    21601 agtaaattgg aattcttttg cctgcttagc cgaaaagaca tcagccgtat gatcttctac
    21661 tttagcatca ggtcttttca taattggggt aaacgtatgc cccggtgctt tggttgaacg
    21721 caacgaaaca taaggggctt gagctgtatc tgttttggtt gcttcagcgt cttgagaggt
    21781 aaccgaagat tcaccaaaaa ccggttcttt gcgaaaatta ttttgtgtca tattaattcc
    21841 aaataatagc gttgttaatg agtataaaaa tggcgatatt ctaaagcaat tcagccgcta
    21901 tttgaattta tttaactaaa tttacgcaac tagcggtcat ttttgcctaa ttttttgcaa
    21961 atctatttac tttcattgaa tttcagacca aatgcaagtc tagaagttta gccattttct
    22021 attttttcga tctgtgtcga aaaatttatt tttgccggtt gagttctatt ttattccctc
    22081 ttaatcttcc tttcgacctt tcaacaacta ataaggaaat cctatgaaga aaatcaaaca
    22141 acttggtcta acattcttgt ttggcttaaa tgcaatggct tttgcccaaa caccgccaac
    22201 tacaatgcct gttgaacaaa atgcagtcac tcaacccgta cagcaaacac aaatcaccaa
    22261 cgctaatgcg gtaaatatca atactgctag tgctgctgaa ttgcaagaca agctagtggg
    22321 aattgggcag aaaaaagctc aagcgattgt cgattaccgt actaaaaacg gcaattttaa
    22381 aaacattgag caacttgctg aagtctccgg aattgggcaa gcaacggtag aaaaaaatcg
    22441 agccaatatt attttagaat aagacaacca aattgttgaa gtggaattcc tacaaagccc
    22501 ctatctgttt ttttctttgt agccacttca acaccttaaa gattttttat ctattttgca
    22561 ttgtatttgc tatacttcgc ccccaattaa acgcaaattc tcgtttttaa ataaaaggaa
    22621 aacgcaatgc aattaaaaaa atatccagtt ccaacctatt tccaacgtgt gaaaatcgct
    22681 ctgcactatt tactgccaca actggcaatt acccgtgcgg caggttggtt agcagagcaa
    22741 aaatggggcg tagttactca ccttatcatc aaactgtttg caaaacagta taaagtcaat
    22801 ttatctgaag ctaaaaaaac cgagccgtca gattatgcta cttttaatga attttttatc
    22861 cgtgaattaa aagaaaacgc acgcccgatt aatcaagatc caaatacact ttgcttaccg
    22921 gcagaaggta aagtcagcga atcaggcgaa atttcggata accgtttatt acaagcaaaa
    22981 gggcatcatt ttacgctaga aaccttattg gcaaatgatg aagaaatggc ggcaaaattt
    23041 aaagacggca catttattac cacctactta tcgccaactg attaccatcg tgtacatatg
    23101 ccgtgcgatg gaacattgcg taaaatgatt tatgtgccgg gcgaattgtt ctccgtcaat
    23161 ccgtttttag ccgaacacgt tccaaactta ttcgctcgca atgagcgtgt gatttgtgaa
    23221 tttgacaccg cattcggacc aatgctacag attttggtcg gtgcaacggt taccgcaagt
    23281 atgagtacgg tttgggctgg cgtaattaat ccgcctcgcg ctgatgaagt ggtagtttgg
    23341 aattacgaaa ccgaaggcga aaaagccatc aaactccaaa aaggcgaaga aatgggagct
    23401 ttccgtttag gctcaaccgt aattaacctc ttcccgaaag gtaaagtaca tctcaaccct
    23461 gcacttattg caggaatgca aacccgaatg ggggaagagt tgggtagaat tatcaattaa
    23521 atcctgagtt aattttacct aaactgtttg ctataatctt ccgataagaa actcttatcg
    23581 gaaggaaaaa tatgtacaca cttcaagaat taaagcagat gaacgagcaa gaaattcgct
    23641 ctcgattaat ctctcctgca attagaaatg caggatggtc ggatagacaa attggcgaag
    23701 agtacacatt taaaaccaat aaacgattta cagatgggca agtagttgtt gatccaaaaa
    23761 cgacgcaaac taaacgcatc gaagcaaaac gggttgatta tctgctctat acttccgcaa
    23821 atcaaaaaat tgccgtggtc gaggccaaag ataaccatca ctcttcccga cacggcttac
    23881 agcaagcaat gacatacgct cgattgcttg atgtcccttt tgcatatagc tcaaatggtg
    23941 atgagtttgt ggaacacgat tttatcactg gcgtacaacg aacccttccg atgtcagcat
    24001 ttcctactcc cgacgaactc caccaacgtt gggcgaaaca atataccacc gaagagctga
    24061 aaattattca acagccttat tacaccagta gcactgacac ctttaaagag cctcgttact
    24121 accaagaaat tgcgattaac cgtactgttg aagctgtagc aaaaggtcag cagaggattt
    24181 tattggtaat ggcaacaggc acgggtaaaa cctatacggc tttccaaatt atccatcgtt
    24241 taaaaaccag tggtgcgaaa aagaaaattt tatttcttgc cgatcgcaat gtactagtcg
    24301 atcagccaat gatgcaagac ttcaaaccgt ttaaagacaa aatggtgaaa gtcagcaata
    24361 aaaagctcga cagtgcttat gaagtctatc ttgcacttta tcaacagctt gacggtgaaa
    24421 ccgataacga actgtttaag cagttccaac cctctttctt tgatcttatt gtgatcgatg
    24481 aatgtcatcg tggttcagca aaagaagatt ctgactggcg tgctattttg aagtatttca
    24541 aggacgccac ccatatcggt atgactgcaa cccccaaaga aaccgatgat gccagcaaca
    24601 ttacctattt tggcgagccg atttatacct atagcctcaa acaaggtatt gaagacggct
    24661 ttcttgcccc ttatcgagtg attcgtgttg atctggactg tgatttaaaa ggctggcgac
    24721 caaccaaagg tttaaaagat catcaaggta atgagattga agatcgcctc tatacagtca
    24781 aagatttcga ttacaacatt gtgctaaatg atcgcacccg cttggttgcg gagagaattt
    24841 gcgaattttt acgcaaaacc gaccgcttgc agaaaacaat agtcttctgt gccaacatca
    24901 accatgcaga gcgaatggtg gcagagcttt cacgcctcaa tgccgatatg ttgaaacaca
    24961 attccaaata tgtctgccaa attacaggcg accaatcagg caaaggcgtt gatttagaag
    25021 aattttgtga tgtagcaaat gaagggaaag attaccctgt gattgccgta acctctaaat
    25081 tgatgacaac aggtgtagat agcaaaacct gtaagctggt tgtacttgat gcaaacattc
    25141 aatcaatgac ggaatttaaa caaattatcg gacgaggcac tcgtcttcgc cctgatttcg
    25201 gcaaagaatt tttcaccatt ttagattttc gaggcgttac tcgcctgttt gctgatccgg
    25261 catttgatgg tgagccggtg gtgattttag atgtaccgga aaataagcca cttgtcgaac
    25321 aaccgcttcc taaacccagt gataaggaca tcattaaaga ccccgagcta aagccatata
    25381 aaatcccggt agttgtcgat ttcggcacac tcaaattacg cattttagac gagaaagtcc
    25441 aatatctgaa tgagcagggc gagctggttt ctgaagatat tgatctgttc tcaaaacggg
    25501 aaatgcaaaa agagttcggc agttacgaac agttccaaca gcaatggaca acaagcggtg
    25561 aaattttctc gaaattttac accgatccaa aatggctggc agcccttcgc caaacacgcc
    25621 aattcagcca cgatgttgaa gactttgatg tattaagcca catctccttt ggcaaaaaac
    25681 cactgactaa aaccgaacga gcagaaaaag tgaaacaaag tggctatgtg gagcagtatt
    25741 cgcccgaaaa ccagcaagtt ttagggttgc tgcttaatga atatgtcaaa tccggcaatc
    25801 aagatctgac taaaaccgaa attttaaaga caccgcaatt cagccgtttt ggaggtctag
    25861 tgtctattat taaaaatttc ggcggtaaag aactctacca gaaagcggta aaaggcttag
    25921 aaaagatgat ttattcggaa taacaagcgg ttattttaca acaaaatttt acagataaac
    25981 aaggatcaca atgagcctca acaacatagt aaaacgcctg caagatgtaa tgcgtaacga
    26041 tgcagggatt aacggtgatg cccaacgcat tgaacagatc gtctggatct tattcttgaa
    26101 aatttacgat gctaaagaac aagagtggga actggaaaac gatgaatatc actcaatttt
    26161 gccgaatttt ttacgctggc aaaattgggc ggaagataaa aaagacggca aagcgatgac
    26221 tggtgatgaa ttactctctt tcgtcaataa cgaacttttc ccgactttaa aaaatctgcc
    26281 tatttcagcc gatacgccga tgaaccaacg gattatccgt gctgcttttg aagataataa
    26341 caactatatg aaaaatgggg tgttattacg tcaggttatc aatatcattg atgaaattga
    26401 ttttgcccat taccaagaac gacacgcctt tggcgatatt tatgaaaata tcctcaaaag
    26461 tttacaaagt gcaggtaatg caggggaatt ttatacccct cgcgctgtaa ccgattttat
    26521 ggtacaagca atcaaaccga aacttggcga acgcattgcc gattttgcct gtgggacggg
    26581 tggtttttta acttcggcgt taaaggtatt ggaaagtcaa attcaaaccc tttctgaccg
    26641 cactttattc aataattctg tgtatggcat tgagaaaaaa gcccttcctc acctgctttg
    26701 tattaccaat ttattgttgc acgatattga taatccgaat gtacatcacg ataatgctct
    26761 agaaaaatcg gtcaaagatt acaccgaaaa tgataaattt gatgtgattt tgatgaatcc
    26821 accttacgga ggttcagaaa ttgagcaaat taaaaagaat ttccctacgg ctttacagag
    26881 cagtgaaacc gctgatctgt ttatgtcggt aattatgtac cgcctaaaac agaatggacg
    26941 ggctgcggtg gtgctgcctg acggattttt gttcggcaca gataatgcca aagttgcgat
    27001 taagaaaaag ctattagaag agtttaatct gcatacgatt atccgcttac cgcatagtgt
    27061 ctttgcccct tatacctcga ttacgactaa tattctgttt tttgataaaa ccgagccaac
    27121 ccgtgaaact tgggtttatc gccttgatat gccacagggt tataaaaatt tctccaaaac
    27181 taaaccgatg aaattggaac atttcaacga agttgttgaa tggtggggaa atcgccaagt
    27241 gattgaagtc gaagggtttg acaaagcgaa aagttttaca ttaaaggaaa tgtccgaccg
    27301 tcaatacaac ctcgatttat gtggctttcc tcacgaagaa gaggaaatcc ttccgcctga
    27361 tgaactaatt gcagattatc agcgaaaacg caccgcttac aatgctgata ttgataagat
    27421 tctagcagaa attaccgata ttttaggcat taaactgtaa taggagcata aaatgctgac
    27481 ttgggaacat atcgaaaaaa tgattgcaca tcagcaattt atccgagaaa aggcagaaat
    27541 tgaatataaa gaggcaaaaa atagtgtgcc aaaagattgg catacttatt cggcttttgc
    27601 aaacagcaat ggtggcttta ttttattagg aataaaggaa aatgaacaag gacattttcc
    27661 tgaactgcta atttctggtg ttaatgatcc tgataaagtc attgatgaat tattttccca
    27721 atctcgtagt gaaaaagtca gtaaatcctt attaaaggat aatgatgtga aaatctttca
    27781 agttgaaaat aaaagcgtga ttgcaattta tgtgaaagca gctgatattt tagaccgtcc
    27841 tatccattta aatggtgatt atcgactttc ttatgttcgt ttaaattcag gcgatcataa
    27901 actcacctcc tctgaactgc gttctttcat ttcaagctat agccaaaaag atgctgatgg
    27961 gagaattgtg ccttatgctt ctattgatga tatcagttta agcacattgg ataaatacag
    28021 aaatctgctg aaagcccata acagcacatc aaaattactt acgttatccg atgagaaatt
    28081 gctgcaagaa attggggctt ataaaaaaga tttagcacag aatagcgagg gcattaccaa
    28141 tgcaggctta ttgatgtttg gtaagcacta tgccattaca agcatatttc cccacttctt
    28201 ttttgaatat tacgaagaag ctggtgatgt aaaacgctat acattacgga ttaccgattt
    28261 tgatcttgaa gaaggcaatc tctttgaatt ttacttgaaa attgtgccac ttattcaaga
    28321 gcttggtaaa gataaacatt tcaaattaga taatttaacc cgtactgagg aaaatgaaat
    28381 tactgacggg ctacgggaag ctctaatcaa tgcaattact cacgcagatt actttaacaa
    28441 tgttcgccat ctaaaagtca ttaaaaccaa agataacaaa ctgatttttg aaaatgcagg
    28501 agtaatgttg gttgatattc ccacggctat tgcagggcaa agatcggaat gccgtaattc
    28561 tattattcat aacttattcc accgtttagg gctatgtgag cgacaaggac aaggcgttcg
    28621 ggatatttat tcatattggc aaagccgaaa tttcaattca ccgcttttag aaagtgaaat
    28681 tgattacact aaactcacct taacttttca atcaggtatg attgcagatt ttgaggaaaa
    28741 atttaccgct tgctttggcg aaaatttctc tgagttacag cctattcaaa aagattgctt
    28801 actctttatc gccttcaatc aaggtaaagc aatgtttgca gaattagcac aaaaactgcc
    28861 ccaatatcca cgcagaaata ttactttggc actagcacga ctgaaagaaa aaggcttact
    28921 aaccagttcg gggagtccga gaaaacaact ctatacccta gtgaatttag aatggataat
    28981 cacagagaaa tcacaagatg attattttaa tccacctttc gaccaacttg ataaagctag
    29041 tgagagtaag ggcgcccccc cgaagaaaag caaagtgaag caagtcaaac aattacccca
    29101 gaacaaagca aacaaattac ccccgaagta aatcgcttac tttcagtgtt gcttgagccg
    29161 atgaagagca aagaaattat gaaaaaactc aggttaaaag atgataagca tttccgtatg
    29221 cagtatttaa aacctgcact aacgcttggt gtgatcgaaa tgcaattccc agaaaaccca
    29281 aaacaccgta atcaaagata ctttaagaaa taacaaccga ttattgagaa taaaaaatga
    29341 aagcccaaca gctaaaaaac gcaatcctac aactagcaat ccaaggcaag ctcgttccac
    29401 aagatccaaa tgatgaaccg gcaagcgaac tcctttgcaa aatccaagca gaaaaagacc
    29461 gcttgattgc agaaggaaag atcaaaaaaa acaaaaaaac tgccgataaa gccccttata
    29521 ccattgaacc accttttgag attcctgaga gttgggtgtg ggtgaggttg ggggatatat
    29581 gcctaaaaat tacagatgga actcatcatt ctcccccaaa tatcgataag agtgattttc
    29641 tttatattac agctaaaaat ataaagaaag atggattaga tttatcaaaa attagctatg
    29701 taacaaaaga aattcataat gaaattttca gccgttgtaa tcctgaaaag ggagatattc
    29761 tatatattaa agatggagcc acaactggag taagtattat aaatactctt aatgagccat
    29821 ttagtatgtt atccagtgtt gccttaatta agacctcaca agaaatagat aatgagtact
    29881 taaattatgt aatgaatagt cattattttt ataacatttc tattggaagt atgtctggaa
    29941 caggtattcc aagaataaca ttaactaaat tagaatctta tttagtccca gttccgccac
    30001 tcctagaaca acagcgcatt gtgcaaaaaa tcgaggaatt attaccgctt gttgaacgct
    30061 acgagcaaac cgaacagcaa ctcactaaac tcaacaatac attccccgaa cagctgaaaa
    30121 aatcggtgtt acacgcagct attcagggta aattaaccga gcaagatccg aatgatgaac
    30181 tggcatcttg cctgattgag cggataaaag cggagaaaaa tcgtcttatt gccgagaaaa
    30241 aactcaaaaa accaaaaagt gtttcagaga ttgtaatgcg tgataattta ccttatgaga
    30301 tcaaagcagg tcaggaacgg tgtatcgccg atgaagtgcc atttgagatt cctcaaaatt
    30361 ggacttgggt gaggttgggg gaagtaggtg attggagttc aggtgcaact ccaaaccgac
    30421 acaatcctga atactataat ggtacgatcc cgtggctaaa aacaggagac ttaaacaacg
    30481 gaataatttc atctatacct gaatttatta cagataaagc attgcaggaa tgttctgtaa
    30541 gattaaatcc tgttggttca gttttgatag caatgtatgg agcgacaatt ggtaagcttg
    30601 ggatattaaa aatagcagca acaacaaatc aggcttgctg tgcgtgtatt ccttttaatg
    30661 gaatttataa taaattctta ttttattatt tgatgtcgca gaaagcagaa tttcagaaaa
    30721 aaagtgaggg aagtgggcaa ccaaatatat caaaggaaaa aattatcaat tatttatttc
    30781 ctttaccacc aatccacgaa caacatcgca ttgttcaaaa aattgaacaa ttatttgcag
    30841 agattgaaaa attgtaggag tcatcatcgc tttcttgggc gtgttggata cgccctacag
    30901 tcactaaatt attgatcttt tatcatatcg gttttcaacc aacctaaaag gaaacctata
    30961 tgatccatct atttgaaaag caccttaatc aatctaatct tgctgaaaat acccaatcag
    31021 cttatctttt tgcggtaaaa caatatcaca aaaaatttga tca
//
LOCUS       DANLZC010000008        30563 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.008, whole genome shotgun sequence.
ACCESSION   DANLZC010000008 DANLZC010000000
VERSION     DANLZC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 30563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30563
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.008"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            5..505
                     /locus_tag="PXE44_000328"
     CDS             5..505
                     /locus_tag="PXE44_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL5587874.1"
                     /translation="MSNIGLDKEIAKKLADELNGLLATYQVFYTNVRGYHWNVRGVNF
                     FELHAKFEEIYDDLVTKVDEIAERILTLGHTPQNAFSQYLTKSQIQEHIGVSAAQECL
                     RGTLNGFKTLLGQQREILALANDANDEGTASQMSDYIKEQEKLVWMFTAACAECSDYA
                     QGCSCN"
     gene            564..1277
                     /locus_tag="PXE44_000329"
     CDS             564..1277
                     /locus_tag="PXE44_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDL5587875.1"
                     /translation="MAMSQGFQFKQFFIRHDRCAMKVNTDGILLGAIAHIKDCQNILD
                     LGIGSGLVALMLAQRTDDCCHITALELEPNAYQQAVENAQNSAWANRISVLQGDVMQQ
                     AFEQKFDLIVSNPPYFADSLAARTTERDLARSISHYSHLNWLAKAKPWLAENGKITLI
                     LPIAAAEKLVDQSEQIGLYCVEQWLIFTKSGKLAKRSIVSFSLNKQKREHKELIIYDL
                     DNCYTTEFKRLTQDFYLNF"
     gene            complement(1388..2392)
                     /locus_tag="PXE44_000330"
     CDS             complement(1388..2392)
                     /locus_tag="PXE44_000330"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL5587876.1"
                     /translation="MAFNLKNRHLLSLVNHTEREIRFLLDLARDLKRAKYAGTEQQKL
                     KGKNIALIFEKTSTRTRCAFEVAAYDQGAHVTYIDPNSSQIGHKESMKDTARVLGRMY
                     DAIQYRGFKQSVVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIENCEKPLSQISYV
                     YIGDARNNMGNSLLLIGAKLGMDVRICAPKALLPEDAFVKMCEKFAAESGARMTVTED
                     IETAIKGVDFVHTDVWVSMGEPLESWTERIDLLMPYRVTPELMQATDNPKVKFMHCLP
                     AFHNSETKVGKEIAEKYPALANGIEVTEEVFESPMNVAFEQAENRMHTIKAVMVASLA
                     "
     gene            complement(2480..3319)
                     /locus_tag="PXE44_000331"
     CDS             complement(2480..3319)
                     /locus_tag="PXE44_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL5587877.1"
                     /translation="MQSYPYLLVNVFATSHFGGNPLAVFYEADGLTDLQMQLIARQFN
                     LSEVVFIQSSTDSQAVKKLKIFTPDYEMPFAGHPTVGAAFVLHSAFDFPTTYRLQTNA
                     GLVAIQHQHNVTTFALTNGVNLENTPLSRAECASILGLNVADIASEPCYVNTGVEQLL
                     VELASQQAVEKCKINANLAEKAFLKDSVYVWCREGNEAKVRLFFQSQGSIVEDPGTGS
                     AAANLGGWHIAQGKIPIQLQISQGDEIQRPNRLLLKVDQNGKIFVGGEVILVGKGEFY
                     LPE"
     gene            3509..4843
                     /locus_tag="PXE44_000332"
     CDS             3509..4843
                     /locus_tag="PXE44_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5587878.1"
                     /translation="MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLS
                     APPISVMLVILAVVTCASILEAAGGLKFMLQVAERILRSNPKRITFLGPLVTYLMTLM
                     LGTGHSVYSIMPIIGDIALKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEIVK
                     LPGFEGVTLLNVVGVTITATFCGVIAMSLYSLRRGKELEDDPEYQRRMQDPELRKQIE
                     QTSATSLDEKLPASAKNSVYLFLAAIATIVIIAMIPAIKPTVAATGKVAGMDQIIQIV
                     MLTFGGLILILTKTDPKKVPNGIVFKSGMVATIAIFGIAWMSDTYFTHAMPAFKEGVT
                     EMVRAQPWTFAFALFAVSVVVNSQAVTAKMLLPVGIAMGLPAPLLVGLMPATYAYFFI
                     PNYPSDIATVNFDVTGTTKIGKYYFNHSFMAPGLVGVITACIVGIAVANVLI"
     gene            complement(4923..5333)
                     /locus_tag="PXE44_000333"
     CDS             complement(4923..5333)
                     /locus_tag="PXE44_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL5587879.1"
                     /translation="MNSTLTNLQPLAFLITRVVAGYMFLLHGTAKFFEFPMSMTNGNG
                     SVPLASIYGVGGILEIVGGIFFILGLFTRQTAFILSGMMAYAFFIAHGAQGNVLLPLL
                     NGGELAGVYSVLFLTYIFIGGGKYSLDAKLFNKA"
     gene            complement(5456..6133)
                     /locus_tag="PXE44_000334"
     CDS             complement(5456..6133)
                     /locus_tag="PXE44_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL5587880.1"
                     /translation="MNLFGFRCFHCNHPLAIGVQGICSHCQKLLKPTPYCRHCGMSAV
                     EFRNACGYCLKNEPKWHKLVRVNEYRAPLSHWVHQFKFQQQYWWDQALARQLLLAIKQ
                     AQREQVLSLPEVIMPVPLFWHRHWKRGFNQSELLCDWLSKWLQIPLDTQSLQRVRATV
                     SQRELTAAERRKNLRGAFFYQPLKAYKRVAIVDDVLTTGSTLNVICAELLKAGVVEIQ
                     VWVLARA"
     gene            6251..7063
                     /locus_tag="PXE44_000335"
     CDS             6251..7063
                     /locus_tag="PXE44_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL5587881.1"
                     /translation="MKSLQKPIKIVFFDIDDTLYVKHKAYIPKSITEQVLPRLKAKGI
                     IPAIATGRNHGCFPEALKPYLNPEIGFELFVTINGQYNFYKDQIISDYSLSREQIERI
                     IEKANALGILYAFITPMTIAVSETNPIVHEAVLPITPNYLIDPHYYEKEKIVQMLLFY
                     TEEQKQAVENSGIFANDLKEARWHPNAVDILRKENSKARGIQDVLATLNIEIESAMAF
                     GDGINDLEMLPTVGVGVAMGNGVPELKAVADFITKPLEEDGILYALETLEVI"
     gene            complement(7111..7380)
                     /locus_tag="PXE44_000336"
     CDS             complement(7111..7380)
                     /locus_tag="PXE44_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5587882.1"
                     /translation="MGLLYLLLLNTLIGYGSYGEALNHWDTSKVSIVTTMLPIFTMIF
                     SNLSYYFYPHIFAKPDMNWISYLGALVVVSGAILAIAGDKLFRRN"
     gene            complement(7353..8030)
                     /locus_tag="PXE44_000337"
     CDS             complement(7353..8030)
                     /locus_tag="PXE44_000337"
                     /inference="COORDINATES: protein motif:HMM:NF013088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5587883.1"
                     /translation="MKQRPLLGFSLALTAAMTWGTLPIAAQQVLKVIDAQTLVWVRFV
                     VASVGLLLILGFAKRLPKLTACSKKELGLILLGVCGLSANFFLFAEALHYVSPTINQV
                     LWQLAPFSMILLGVLLFKEKFGLFQKIGFVLLIAGLVAFFNDKFAEILQLNTYALGVL
                     LSAGASLIWVLYGVAQKLLLAKFNSQQVLFIIYIGCSLLMLPFASPAQLGNLEDGFLW
                     GCFIYCC"
     gene            complement(8132..8389)
                     /gene="rpmA"
                     /locus_tag="PXE44_000338"
     CDS             complement(8132..8389)
                     /gene="rpmA"
                     /locus_tag="PXE44_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL5587884.1"
                     /translation="MATKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGSNVGMGKDHTLFATADGKVKFEVKGEKSRKYVSIIAD"
     gene            complement(8410..8721)
                     /gene="rplU"
                     /locus_tag="PXE44_000339"
     CDS             complement(8410..8721)
                     /gene="rplU"
                     /locus_tag="PXE44_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL5587885.1"
                     /translation="MYAVFQSGGKQHRVSEGQVVRLEKLELATGEKVEFDSVLMVVNG
                     EDVKIGAPVVAGAKVVAEVVAQGRGEKVKIVKFRRRKHSRKQQGHRQWFTEVKITGIQ
                     A"
     gene            8999..9997
                     /gene="ispB"
                     /locus_tag="PXE44_000340"
     CDS             8999..9997
                     /gene="ispB"
                     /locus_tag="PXE44_000340"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDL5587886.1"
                     /translation="MAVQLFSLEQIQALANEDMQAVNAEILAQLNSEVLLINQLGHYI
                     IAGGGKRIRPLIAVLAARALGYRGKKHITAAAFIEFVHTATLLHDDVVDESDMRRGRP
                     TANAHFGNAASVLVGDFIYTRSFQMMTDLDSLRVLKVMSAATNALAEGEVQQLMNVND
                     PNTTEANYMRVIYNKTARLFEAATQCVAIAADASEEIETALKNYGCYLGTAFQLIDDI
                     LDYSAQSESLGKNVGDDFVEGKPTLPLLHAMHAADKAGKTTQAELIRNAIENGGELEQ
                     IPQVLAIMQEHQSLDYAMQKAREEAQKAVDSLGVLPESEYKQALVSLAYLSVDRSY"
     gene            10100..10846
                     /locus_tag="PXE44_000341"
     CDS             10100..10846
                     /locus_tag="PXE44_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL5587887.1"
                     /translation="MTEQNQRPQEHIESVRARRRAQNQKKGPNAPFVREKLTLPNGHN
                     KLLLHSCCAPCSGEVMEAIHASGIEFTIYFYNPNIHPLKEYLIRKEENIRFAEKWGIP
                     FIDLDDDYENDRKEWFKKAKGMEWEPERGIRCTMCFDMRFEKAAEYAHNNGFPVFTSC
                     LGISRWKDMNQINDCGHRAAAKFDDVVYWDYNWRKGGGSQRMIEISKRERFYQQEYCG
                     CVYSLRDSNKWRLETGRQRIEIGKLYYSPD"
     gene            11263..12198
                     /gene="fabD"
                     /locus_tag="PXE44_000342"
     CDS             11263..12198
                     /gene="fabD"
                     /locus_tag="PXE44_000342"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL5587888.1"
                     /translation="MSKFAMVFPGQGSQAVGMLADLAPQYPIVEETFKQASDVLGYDL
                     WDLVQNGTAEELGQTHRTQPALLASSVALFRIWQQKFPEQKPAVMAGHSLGEYSALVC
                     AGVLDFQDAIKLVELRGRAMQEAVPAGSGAMFAIIGLDNESIIKACEQAASETGEIVS
                     AVNFNSPGQVVIAGTKAAAEKAGELCKAAGAKRALPLAVSVPSHCALMKPAAEKLAEA
                     LQNIELNQPLVPVINNVDVAVETDADTIRNALVRQLYSPVRWTETVEKMAQDGITTLY
                     EIGPNKVLTGLAGRIVKELSAKAVNDVASFEAVEA"
     gene            12215..12679
                     /locus_tag="PXE44_000343"
     CDS             12215..12679
                     /locus_tag="PXE44_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587889.1"
                     /translation="MSKETLFMLISALIALVILPLAYTVGIAKETLGGNMGMLSIAAG
                     CSFLAYFLSLNPKIRHAFIGGSAITAAAIQAWVLYGVLLSENDPKSAVVLFQIYLFSS
                     LGPLFFSAIWAFTRPRWIYPKQGSAVKYALFGGLTTVINYLITLFIFYILTL"
     gene            12700..13422
                     /gene="fabG"
                     /locus_tag="PXE44_000344"
     CDS             12700..13422
                     /gene="fabG"
                     /locus_tag="PXE44_000344"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL5587890.1"
                     /translation="MSKIALVTGATRGIGKAIAEELVAKGYIVVGTATSEKGAESISA
                     YLGENGKGLVLNVADAESIESVLKQIKETFGDVDVLVNNAGITRDGLLMRMKDEDWFD
                     IIQTNLTSVYRLSKAVLRPMMKKGGRIITIGSVVGSMGNPGQTNYCAAKAGLVGFSKS
                     LAKEVASRGITVNVVAPGFIATDMTDELTEDQKNAILSQIPAGELGSPQDIAKAVAFL
                     ASDDARYINGETIHVNGGLYMS"
     gene            13541..14839
                     /gene="brnQ"
                     /locus_tag="PXE44_000345"
     CDS             13541..14839
                     /gene="brnQ"
                     /locus_tag="PXE44_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL5587891.1"
                     /translation="MTRNTLVVGFLLFAIFFGAGNLIFPPKLGYESSTEFFSAISGFV
                     LTGVGLPLLGIIVSAFYNGGYKSALDKIHPWFSLAFLTSIYLAIGPFFAGPRTAATAY
                     EMAIMPFLGDSSQVSMLIFTLIYFAFTLWLALNPNKMVDRIGAILTPILLITIIGLVI
                     RAGFILDTDPQITTPNTNEQSYFFKGMVDGYLTMDALASLAYSVIVLNAIRGKGVSQA
                     ALQRQTVSAGLVAAVALAVIYISLGWIGNNMPISANTLSMLSERKLDLGTYILNTVTT
                     QAFGELGRSVLGVIVSLACLTTAVGLAVSVSEYFNEIFPKISYKVYAILFTVISFTVA
                     NQGLKEVISKSIPVLLVLYPIAMTVILMLLINLVIKLPLSAQRLSLLLVTIVAIASVA
                     GVGFTENLPLKSYSMEWLPFAIIGAIIGAIIHPLTGRKHK"
     gene            14885..15709
                     /gene="mutM"
                     /locus_tag="PXE44_000346"
     CDS             14885..15709
                     /gene="mutM"
                     /locus_tag="PXE44_000346"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDL5587892.1"
                     /translation="MPELPEVETSVRGVSPYLVGQTIEQIIVRQPKLRWTVSPELSQM
                     AGAKITAVERRAKYLIIRTDKGDILVHLGMSGSLGILQERQQKEIGKHDHVDLITQNG
                     TILRYNDPRKFGCWLWAEKAEDHELLRKLGPEPLSNDFTADYLFAKSRKKTTACKTFI
                     MTNEVVVGVGNIYACESLFMAGIHPELAAQNLTKKQCERLVKTIKEVLTKAIIQGGTT
                     LKDFIQPDGKPGYFAQVLQVYGRKGETCNDCGKPIETKVIGQRNTYFCPKCQRLPK"
     gene            complement(15792..16226)
                     /gene="dtd"
                     /locus_tag="PXE44_000347"
     CDS             complement(15792..16226)
                     /gene="dtd"
                     /locus_tag="PXE44_000347"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL5587893.1"
                     /translation="MIGLIQRVKFAKVEVEGQVVGEIASGLLVLLGVEKDDDEAKADK
                     LLEKVLNYRVFSDSEGKMNLNVQQAGGSLLVVSQFTLAADTNKGLRPSFSKGASPVDA
                     ERLYAYFHQQAATKIHTQTGQFAADMQVSLQNDGPVTFWLQV"
     gene            complement(16201..17046)
                     /locus_tag="PXE44_000348"
     CDS             complement(16201..17046)
                     /locus_tag="PXE44_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDL5587894.1"
                     /translation="MLNRLWRFIKLFAKRYNENNIAISAGYLTYSTMLAIVPFIVVLF
                     SIFTFFPIFHEANEQLKAFIYDNFAPSASEMVSEYIELFVSNAKQMGLVSFIGLFVVT
                     IMLIKSIDEALNTIWHDNRQRPMWFSFLLYFLILLFGPLLAGASIAISTYVMSLSIFN
                     GNGALSLGQHLLELMPFLLIWLLFTLVYKFIPIRKVKFQHAAIGALMAAIFFTLGKKA
                     FIWYITAFPSYQAIYGALAVLPIMLLWIQLSWQVVLLGGQFAAVLDEMETDKGSINDR
                     LNSAG"
     gene            complement(17048..18286)
                     /gene="proA"
                     /locus_tag="PXE44_000349"
     CDS             complement(17048..18286)
                     /gene="proA"
                     /locus_tag="PXE44_000349"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL5587895.1"
                     /translation="MQMIELAQQAKQASFELAQFGNLQKNQALLTIADSLEQRAEEIL
                     YANAKDIEFAQAQGISLAIIDRLLLNEDRLKDIANDVRNVAKLADPVGQVMDGGVLDS
                     GLKIERQRVPLGVILTIYEARPNVTIDVASLCLKTGNAVILRGGKETKFTNAVLVDVV
                     QDALEKAGLPKLAVQAVTDPDRALLLELLKLDRYIDMVIPRGGAGLHQFCKENSTIPV
                     IVGGIGVCHLFVEKTADQEKSLEVIANAKTQRPSTCNTLETLLVEKAIAVEFLPKLVA
                     KMQQLGVTIHSNDLQKIEGIEPLDQAKLSQEWLSLDLNVVVVNDLQQAVEHIRHYGSQ
                     HSEGILTSDYQLARQFVQQVDAAAVYINASTRFTDGGEFGLGAEVAVSTQKLHARGPM
                     GLEALTTYKWVCEGDYLVRG"
     gene            18570..20453
                     /gene="mutL"
                     /locus_tag="PXE44_000350"
     CDS             18570..20453
                     /gene="mutL"
                     /locus_tag="PXE44_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL5587896.1"
                     /translation="MINTTPKQLIHILPPQLANQIAAGEVVERPASVVKELVENSLDA
                     GANTIQIDIEKGGSQLIRIRDNGCGISKQDLPLALARHATSKISSLEDLEAILSLGFR
                     GEALASISSVSRLTLTSRTAEQTEAWQAYAQGREMAVEIQPASHPIGTTIEVANLFFN
                     TPARRKFLRTDKTEFAHIDEVVRRIALAKPNISFSLSHNGKIVRQYRKVQDNSVEQQQ
                     KRVAAICGDEFIQHSTYIDRQHGDLHLHGWVGSPALARLQNDLCYSYVNGRMMRDKTI
                     NHAIRQAYGDSIASGYYPAFVIFLDLDPTMVDVNVHPAKHEVRFHQGRLVHDFILQGV
                     LNSLQQTQPTLELRNEVNEPLPSYVQDTNRQAAGQNIFVSQTAPQAAEFTQKNASRSS
                     YTPKFERSNSVSSSAQKWYKELVGGDLHQTTPTIKTQSTLVEPKTEPQTEHKTEAVSQ
                     KITQIAPLASHHSQALAIVKNQAVLIKEEENFYLLPLATLAELKLKLQLEKGQSQALL
                     IPLNLSLDQQQTERWQQTKEELTKLGFSISEKQWQAQTRLTVLSVPQAVREQNLQQLL
                     FALFNQTQAVNLAEFFAKKCEKPTACTLSDVIALLSEIELYANGKKELEGIRKEVDFS
                     KFL"
     gene            complement(20472..20741)
                     /locus_tag="PXE44_000351"
     CDS             complement(20472..20741)
                     /locus_tag="PXE44_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL5587897.1"
                     /translation="MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYC
                     DHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLRIGSHSHLFG"
     gene            complement(20728..20994)
                     /locus_tag="PXE44_000352"
     CDS             complement(20728..20994)
                     /locus_tag="PXE44_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL5587898.1"
                     /translation="MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATE
                     KRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADLMEQLNARD"
     gene            21434..22387
                     /gene="miaA"
                     /locus_tag="PXE44_000353"
     CDS             21434..22387
                     /gene="miaA"
                     /locus_tag="PXE44_000353"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL5587899.1"
                     /translation="MNKKPLALFLMGPTASGKTDLAIALRQELPVEVISVDSALIYKG
                     MDIGTAKPSKEELALAPHRLIDILDPAESYSAMNFHADALREMADITSQGKIPLLVGG
                     TMLYYKALLEGLSPLPSADPAIRAEIESRAAQHGWATLHQELVKIDPIAGARINPNDS
                     QRINRALEVFYITGKTMTELTAQQGEALPYNILQFAIAPQDRAVLHQRIEQRFHKMIE
                     LGFEEEVKRLFLRKDLHINLPSIRCVGYRQMWEYLQGDISLDEAIFKGICATRQLAKR
                     QITWLRGWQGELTWLDSLAPTSSKAKMVEKIDHYLKNCDNI"
     gene            22456..22728
                     /gene="hfq"
                     /locus_tag="PXE44_000354"
     CDS             22456..22728
                     /gene="hfq"
                     /locus_tag="PXE44_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDL5587900.1"
                     /translation="MAKGQSLQDPYLNALRRERIPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPARALSHNNNNQAQHAQPAAEVTEQAE"
     gene            22748..24133
                     /gene="hflX"
                     /locus_tag="PXE44_000355"
     CDS             22748..24133
                     /gene="hflX"
                     /locus_tag="PXE44_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL5587901.1"
                     /translation="MLNESLPNSEENSAFGFALSEASQTTEAASPTDPENQDKAILVH
                     LYLGQHKDVENLLEFKTLAESAGVEVLATLTTSRQSSHIKYYVGQGKAEGIAEAVERL
                     GATVVLVNHQLSPAQTRNLQSLCGCRVVDRTGLILDIFAQRARSHEGKLQVELAQLRH
                     LSTRLVRRVTNQDQQKGGAVGLRGPGETQLETDRRLIKVRIQQLLNRLEKVSKQRNQN
                     RKTRQKADIPTVSLVGYTNAGKSTLFNAITQASVYAADQLFATLDPTLRRIQVQDVGT
                     TILADTVGFIRFLPHDLVSAFKSTLQETTEATLLLHVIDGSDERKNENIDAVNQVLDE
                     IGALDIPTLLVFNKIDKLEGVEPHIERNADGTPVAVYLSAQENSGIELLFQAIQERLR
                     SNLVCENLLLPVTAGAIYAQFKAENCIKTEHFNEFGERIISIEINEVQWNKWVKQFPD
                     LAEYIELAKWG"
     gene            complement(24355..30426)
                     /locus_tag="PXE44_000356"
     CDS             complement(24355..30426)
                     /locus_tag="PXE44_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5587902.1"
                     /translation="MNKIFKVIFNQTTQKMEVVSELARSQGKAASSTDKRGTIKLAAA
                     SILGGATLISGMAAFMLSATPAEAAVIIDRIEVQQKAPKKVVNGGIDIYTNTSLATDS
                     ITNLYNYFNPGSLSYADRTKLQESNNSVNKEKDSRLLYKSNTTSAIVIGHNAVALDNN
                     RKSRGKPKASGIAIGDYSYSGGGLSIAIGSFSRTASIGSVALGTSARAEGFNSFAAMR
                     QSAAIADYSTAIGSTSWANATASFAIGASATALGDRSFAIGSGTPQTVDGSYGHENRR
                     SIYDGQNNTLAFGTDAFSIGTKAKTNGNHSFAIGTNAWSGGFKLENSNISELGQVINP
                     DTAKVANNAFALGTNTRANSNDTLAFSTNASATGDGSIAFGVNSNTTGIGAIAFGNAS
                     KAWKSNAISIGWNANSHAEKVISIGSHSNASKDGAMAIGDTANSSGNNSVAIGYSANV
                     SKANATAVGTRANITHEGGTAIGFEAKSKGNYATAIAYNSTAKGKNSIAAGVNSTVSG
                     IRAAALGYNNTVSGKDTFVLGSNVTVKTKGSVILGSDSTEATATKEDTATVGTLKYSS
                     FAGDENVVDGDYVSVGNATNARQIKFVAPGKIASNSTDAINGSQLYAVADTLVKTGFN
                     ITADHSGLGGGAKEDNVKLGETVKYTSTDKNVVTTVTNNTIDFGLSDNISVGGKPGKD
                     GSVGVKGADGKDGVTIKPEAIVFHGVDGVNGKDGKDGKASIKVEKGAKGLDGNDGKDG
                     ESKTRIVYEKPNGDKEEVATLNDGLKFGANDGKVHDAKLNTQVDIKGATTNTDWAKFD
                     AGKNIMTQVVGNTITVALAKDLAGLDSATFGTQGDSTVINKDGVTIAKKDPNDATKSN
                     VTLTEDGLSNGGNQITNVDSGLKGKDGQPVKLTEAAGDVLNNAVNVGDLKNTVTETVT
                     EVTTDAFGLKDENNKEFKQNLGTTAKIIGDGNVKTNVVEKDGGHALEVSLVNNITVGK
                     DGEPGKVGIAGKDGKDAIALDGKDGAITVGRPGKDGIDGTIGVNGKDGSAVVLNGKDG
                     SIGLNGTNGKDGLTIKGTEGKPGLVGKDGLPGKDGVTRIQYQPTDGNGDPIGEPEQVA
                     TLNDGLHFKGDDATLVSKKLNETLEIIGGADKDKLTANNIGVNSKDGKLSVQLAKDLK
                     DLNSATFKPQDGENGQEGKGPTTVVNNNGVTITPKDGTDGKAVKLTEDGLDNGGNQIV
                     NVKSGIDGLAGKDGINGKDGKPITSVNDLTDDQLKEVGTNAANINDVVNASNDIIEKG
                     FSIAADNGETDHVKLGETVKYTSKDKNIITTVSDNVIDFTLNNDFTIGGPGKDGSIGV
                     KGADGTVGVTLNGKDGSIGLTGPKGADGKDGASATISVKDGAKGLDGNDGKDGESKTR
                     IVYEKPDGTTEEVATLNDGLRFVGDDGKEIVKKLNETLKISGGDTDLTNLSDNNIGVV
                     NNNGALTVKLAKDIDLTKDGSVTIGDTTVNNDGLTIKDGPSVTKAGINAGDKKIINVQ
                     DGEISATSKDAVNGSQLYQVQEVANAGWNLTANGKDKGNVKPGATVDLNNKDGNIIIT
                     KEGNNVTFGLKNNLTIGGKDGKDGQIGIAGKDGKDGVTIKGDGTITAGRDGKDGVDGS
                     IGATGKDGASVVLNGKDGSIGLTGPKGADGKDGASANIAVKDGAEGVDGTNGKDGLPG
                     ENGKTRIVYETKDKDGKTVTEQVATLNDGLIFSGNNEDVKNRQKLNTEVKVKGEGVDK
                     TASENFKSASGNINVKANGTDTLEVQLAKAIDLTEDGSVTTGDTTVNNNGLTIKDGPS
                     VTKGGIDAKGTKITNVKDGDVTATSKDVVSGSQLYNAMQNTGWYLTVDGNVAETSEKG
                     AQRVNNNGKVAVSGGKNIVVSRKGSTVEVATSSNLEFDSVKVGGTTISSTVAKDGVNE
                     LNIAGSKNAQTRITNVAPGVKGTDAVNVNQLKGAENRLNNRISNVDKDLRAGIAGALA
                     AGNLYHVTQPGKSMVSAGVGAYRGQGAIAVGYSRLSDNGKVGIKFSVNSNSRGDTGAA
                     ASVGYQW"
BASE COUNT         8933 a         6805 c         5984 g         8841 t
ORIGIN      
        1 ggcaatgtca aatatcggtt tagataaaga aatcgcaaaa aaattagcag atgagttaaa
       61 tggtttatta gctacttacc aagtgtttta cactaatgta cgtggctacc attggaatgt
      121 acgtggtgtg aacttctttg aattacatgc aaaatttgaa gaaatctacg atgatttagt
      181 gacaaaagta gatgaaattg cagaacgtat tttaacctta ggtcacacac ctcaaaatgc
      241 gttcagccaa tatttaacca agtcacaaat tcaagaacat attggtgttt cagctgcaca
      301 agaatgttta cgtggtactt taaatggttt caaaacatta ctgggacaac aacgtgaaat
      361 tcttgctcta gcaaatgatg caaacgatga aggtactgct tctcaaatga gtgattacat
      421 caaagaacaa gaaaaattag tatggatgtt cactgcagct tgtgctgaat gttcagacta
      481 cgcacaaggt tgttcttgta actaagttgc aataatataa ttaacccccg ataatgaaag
      541 attatcgggg gttacttttt cagatagcta tgtcgcaagg atttcaattt aaacagtttt
      601 ttattcggca cgacagatgt gcaatgaaag tcaatacaga tggtattcta ttgggagcga
      661 ttgctcacat aaaagattgt cagaatattt tagatctggg tataggttct ggtttagtag
      721 cacttatgct tgctcagaga acagatgatt gttgtcacat tactgcgttg gaacttgaac
      781 caaatgccta ccaacaagcg gtcgaaaatg cccaaaattc tgcatgggca aatcggattt
      841 ccgttttgca aggtgatgtg atgcagcagg catttgaaca gaaatttgac ttgattgtta
      901 gtaacccacc ttattttgca gattcacttg cagctcgaac cacagagaga gatttagctc
      961 gctcaattag ccattacagc cacttgaatt ggctggcaaa agcaaaacca tggttagcag
     1021 agaacgggaa aattacactg attttaccta tagcagctgc agaaaaatta gttgaccaaa
     1081 gtgaacaaat tggattgtat tgtgttgaac aatggttaat ttttaccaag agcggcaaat
     1141 tggctaaacg cagtatagtg agttttagtt taaacaagca aaagcgtgaa cataaagagc
     1201 tgatcatcta tgatctagat aattgttaca ctactgagtt taaaagactt acccaagatt
     1261 tttatttgaa tttttaattt actccccggg ttagtgtttt ccacaaatct tcttcagcta
     1321 cgatgatcta gccttaagct aaaaagcaga ttaccaccca agataatccg ctttttagac
     1381 ttagctatta tgccaaactt gctaccatta ccgctttaat agtgtgcatt cgattttctg
     1441 cttgctcgaa tgccacattc attggcgatt caaacacctc ttcggttact tcaatgccgt
     1501 ttgccagtgc cggatatttt tcggcgatct ctttaccgac tttagtttca ctgttgtgga
     1561 acgccggtaa gcagtgcata aatttcactt tcgggttgtc ggttgcttgc attaattcag
     1621 gcgttactcg gtaaggcatt aataaatcta tacgttcagt ccagctctct agcggttcac
     1681 ccattgaaac ccaaacatca gtatgcacaa aatcgacacc tttaattgca gtctcaatat
     1741 cttcggttac cgtcattctt gcccccgatt cagctgcaaa tttttcacac attttcacga
     1801 atgcatcttc cggtaataag gctttcgggg cacaaattcg cacgtccatc cccaatttcg
     1861 ccccaatcaa caggagtgaa ttacccatat tattgcgagc atcgccgata tagacataac
     1921 tgatttggct aagcggtttt tcgcaatttt cgatcatcgt tagcacgtca gccaacattt
     1981 gggttgggtg gaattcatcg gttaagccgt taaacaccgg cacgccggca taatcggcaa
     2041 gctcttgtac taccgattgt ttaaagccgc gatattgaat cgcatcatac attctgccta
     2101 gcactcgggc ggtatctttc attgattctt tatgcccgat ttgcgaggag ttcgggtcaa
     2161 tataggtaac gtgagcccct tgatcgtaag ctgctacttc aaaggcacaa cgagtacggg
     2221 tcgaggtttt ctcaaaaatt aaggcaatat ttttgccctt taatttttgc tgctcagtgc
     2281 cggcatattt cgcccgtttt aaatctcttg ctaaatccaa caaaaagcga atttcacgct
     2341 cagtgtggtt tactaaactg agtaaatgtc tgttttttaa gttaaatgcc attttgtttc
     2401 tccctgtaaa cattaaaatt tattttatgc aaaataattg cataaaaatt cacttatagc
     2461 aaacagaatt tgcttaccct cattcgggta aataaaactc gcctttgccg actaaaataa
     2521 cctctccgcc aacaaaaatc ttcccgtttt gatcgacttt taataacagg cgattcggcc
     2581 gttgaatttc atcaccttgt gagatctgaa gctggattgg tattttgcct tgtgcaatgt
     2641 gccaaccgcc taaatttgcc gctgccgaac cggtgccggg gtcttcgaca atcgaacctt
     2701 gcgattgaaa aaacagtcgc actttcgcct catttccctc acgacaccaa acatatacac
     2761 tgtctttcaa aaatgctttt tcagccagat ttgcatttat tttgcacttt tcgaccgctt
     2821 gttgagaagc taactcaacc aataactgct ccacgccggt gtttacataa caaggctcgg
     2881 acgcaatatc cgcaacattc agccccaata ttgaggcaca ttctgcacgg cttagcggtg
     2941 tgttttccag gttcacacca ttggttaaag caaaagtagt cacattatgt tgatgttgaa
     3001 tcgctaccaa tccggcatta gtttgcaagc gataggttgt tggaaaatca aaggcggaat
     3061 gcaacacaaa agccgctcca acagttgggt ggccggcgaa cggcatttca taatcaggcg
     3121 taaaaatttt gagttttttg accgcttgtg aatcggtgga agattgaata aataccactt
     3181 cagaaaggtt aaattggcga gcaataagtt gcatttgtaa gtcggttaat ccgtccgctt
     3241 cataaaatac tgcaagcggg ttgccgccaa aatggcttgt tgcaaacaca ttcaccagca
     3301 aataaggata gctctgcatt tatcactcct tacataaact taaagccaca ttgggcgaaa
     3361 attctaccat tttggtggcg aatcgtaaaa ttggaaaatt tatcggtatc cctctttaga
     3421 gttaactatt tttgatttat ctcaaagttt taaaaaaaag attaggtcat acttctttct
     3481 agtgattatt ttttaatttg aggttcttat gctttatgca gaatttttac ttttattagc
     3541 ctttttatat gcaggtagcc gttacggtgg catcggttta ggtgtggttt ccggcatcgg
     3601 cttatttgtt gaagtgttct tcttaggaat gccgctttcc gctccgccaa ttagcgtgat
     3661 gctcgtgatt ttggctgtag ttacttgtgc ctccattcta gaagccgctg gcggtttgaa
     3721 atttatgttg caagttgccg aacgcatttt gcgtagcaac ccaaaacgta ttacctttct
     3781 tggcccactt gtcacttatc tgatgacctt aatgctcggc acagggcatt cggtttactc
     3841 tattatgccg attatcggtg atattgcctt aaaaaataaa attcgcccgg aacgcccgat
     3901 ggcagcagcc tctgttgctt cacaattagg tattaccggt agcccacttt ctgctgcagt
     3961 tgcctattac ttaaccgaaa tcgttaaatt accgggcttt gaaggggtga ctttattgaa
     4021 tgtagtcggc gtaaccatca ccgcaacctt ctgtggtgtg attgcgatgt cattatatag
     4081 cttacgtcgt ggtaaagagt tggaagacga tcctgagtat caacgccgta tgcaagaccc
     4141 tgagctacgc aaacaaatcg agcaaacgag tgcaacgtct ttagacgaaa aactaccggc
     4201 aagtgcgaaa aactcggttt atctgttcct agccgctatt gcaaccattg tgattattgc
     4261 aatgattccg gctattaaac caacggttgc tgctacagga aaagttgccg gtatggatca
     4321 aatcattcaa atcgtgatgc taactttcgg gggtttaatc ttaattttaa ctaaaaccga
     4381 cccgaaaaaa gtaccaaacg gcatcgtttt caaatccggt atggtagcaa ccattgcgat
     4441 tttcgggatt gcttggatga gtgatactta cttcacccat gcaatgccag cgttcaaaga
     4501 aggggtaaca gaaatggttc gagcccaacc ttggacattc gccttcgcct tgtttgcggt
     4561 atctgtagta gtaaacagcc aagccgtaac cgcaaaaatg ttattaccgg taggcattgc
     4621 aatgggcttg cctgctccat tattagttgg tttaatgccg gcaacttatg cttacttctt
     4681 tattccgaat tatccgtctg atatcgcaac cgttaacttt gacgtaaccg gtacaactaa
     4741 aatcggtaaa tattacttca accacagctt tatggctccg ggtttagttg gcgtaattac
     4801 tgcctgtatt gtcggtatcg ccgttgcaaa tgtgctaatt taattttaac aaaaaaacaa
     4861 gcggtcagat taagtaaatt ttttaccaaa tctgaccgct tgattattta ctgcaaaacc
     4921 aattaggctt tgttaaagag ttttgcatct aacgagtatt ttccgccacc gataaaaata
     4981 taggttaaga ataataccga atacacgcct gctaattcac cgccgtttaa taaaggtaat
     5041 agcacattgc cttgagctcc gtgagcaata aagaacgcgt atgccatcat acctgaaagg
     5101 ataaacgctg tttggcgggt aaatagacct aaaataaaga aaattccgcc cacaatttcc
     5161 aaaataccgc ctacaccata aatggaggca aggggtacac tgccgtttcc gttggtcatc
     5221 gacattggaa attcaaaaaa tttcgctgtg ccgtgtaata aaaacatata accggcaaca
     5281 acacgggtga ttaaaaacgc taaaggttgt aaatttgtta aagtagagtt catttgcttt
     5341 ccttaaaata aattgtgata taaaggtcaa ttgattgagg caggcattct attttaaaaa
     5401 cctatacttc acaatccagc cgtcatgaaa tagattattt accattaata aacaattagg
     5461 ctctcgctaa cacccaaacc tgaatttcta ctactccagc tttgagtaac tcggcacaaa
     5521 ttacatttaa ggtggaacct gtggtgagca catcatcaac aatcgccact cgtttatagg
     5581 cttttaacgg ttgatagaaa aatgccccac gcagattttt ccgccgttca gcagcggtca
     5641 attctctttg tgagacggtt gctctcacac gttgtaaact ctgggtatcc agcggaatct
     5701 gtaaccactt cgataaccaa tcacacaata attcgctttg attaaaccct cttttccaat
     5761 gtctgtgcca aaaaagtggt acaggcataa tcacttcagg caagctcaga acctgttccc
     5821 tttgagcttg tttgattgcc agcagcaatt gtcgggcaag ggcttgatcc caccaatatt
     5881 gctgctggaa cttaaattga tgtacccaat gtgaaagtgg cgctcggtat tcatttaccc
     5941 tcactaattt atgccacttc ggttcatttt tcaaacaata gccacaagcg tttcgaaact
     6001 ccaccgcact cattccacaa tgacgacaat aaggggttgg ttttagcaat ttttggcaat
     6061 ggctgcaaat gccttgcacg ccaatagcga gtggatgatt acaatgaaag caacggaagc
     6121 cgaataaatt cacatttcct ccttttattt gcagattatc aaatttagcc ttattttttc
     6181 cggctatttt tgtgacatcg ctcgaaaaaa gagtaaaatc tcattatcag taaataaaaa
     6241 ggaaaccgag atgaaatccc tccaaaaacc gatcaaaatt gtctttttcg atattgatga
     6301 caccctctat gtaaaacata aagcctatat tccaaaatca attaccgagc aggtattacc
     6361 tcgtttaaaa gccaaaggca ttattccggc cattgccacc ggcagaaatc acggctgttt
     6421 tcctgaagct ctcaaacctt atttaaaccc tgaaatcggt tttgagctgt ttgttaccat
     6481 taacgggcaa tataatttct ataaagatca aataattagt gattattcgt taagccgtga
     6541 gcaaattgaa cgtattatcg aaaaagcgaa tgctttgggt attctgtatg cctttattac
     6601 gccaatgacg attgcagttt ccgaaaccaa tccgattgtg cacgaagcgg ttttaccgat
     6661 cacaccgaat tatttgatcg atccacacta ctacgaaaaa gagaaaattg tgcaaatgtt
     6721 actgttctac accgaagagc agaaacaagc ggtcgaaaat agtggcattt ttgcaaatga
     6781 tttaaaagag gctcgctggc accccaatgc ggtagatatt ctgcgaaaag aaaattcgaa
     6841 agctcgtggc attcaagatg tattagcaac cttgaatatt gagattgaaa gtgcgatggc
     6901 atttggcgat ggtattaacg acttggaaat gctcccgacc gtaggtgttg gtgtggcaat
     6961 gggtaatggc gtgccggaat taaaagcggt ggcagatttc atcaccaagc cgcttgagga
     7021 agacggcatt ctatacgctc ttgaaactct tgaggtgatt taaaagcaag cggttgtaag
     7081 tttttctcca tttacaaccg cttgtagttt ctaattacgc ctaaatagct tatcgcctgc
     7141 aatcgctaaa atcgccccac tgacgaccac caacgcccca agataactga tccaattcat
     7201 atccggcttg gcaaaaatat gcggataaaa atagtagcta aggttggaaa agatcatcgt
     7261 aaaaatcggc aacatcgtgg ttacaatgct aacctttgaa gtatcccaat gattcaacgc
     7321 ctcaccgtat gaaccgtagc caattaaggt gttcaacagc aataaataaa gcaaccccac
     7381 agaaaaccat cctcaagatt gcctaactgt gccggtgagg caaacggtaa catcagcaga
     7441 gaacagccga tgtaaataat aaacagcact tgctgagagt taaatttcgc taataataat
     7501 ttttgagcca cgccgtatag cacccaaatc aagctggctc cggcactcaa taacactccg
     7561 agagcgtagg tgttgagctg taaaatttca gcaaatttat cgttaaaaaa tgcgacaagc
     7621 cctgcaatca ataacacaaa tccaatcttt tgaaacaagc cgaacttttc cttaaacaat
     7681 aatacgccta gtagaatcat cgaaaatgga gctaactgcc acaatacttg gttaatggtg
     7741 ggcgaaacat agtgtagagc ctctgcaaac aaaaagaaat ttgccgacag accacacacc
     7801 cctaacaaaa ttaaaccgag ttcttttttg ctacaagcgg tcaatttggg taaacgtttt
     7861 gcaaatccta gaatcagcaa tagccctact gatgccacaa cgaacctaac ccaaactaaa
     7921 gtttgggcat ctatcacttt caatacttgc tgggcggcaa tcggtagcgt tccccaggtc
     7981 attgctgcgg ttaatgcaag tgaaaagcca agtaaggggc gttgtttcat cgtggaaatt
     8041 cctttttcta ttctatgttt ctatatttta gaaagattga gatacaaaaa aaccccgcca
     8101 ttggcgaggt ctcttattga ttttgaaatg attagtcagc aatgatgctt acatatttac
     8161 ggcttttctc gcctttcact tcaaatttaa ctttaccgtc agcagttgca aataaagtgt
     8221 ggtctttacc catacctaca tttgaacctg cgtggaattt agtaccacgt tgacgaacga
     8281 taatgctacc tgctaataca gattcaccac cgaaacgttt aacaccaagg cgtttagctt
     8341 cagaatcacg accgttacga gttgaaccac cagctttttt agttgccatc ttctagatct
     8401 cctctgaaat tatgcttgaa tcccagtgat tttcacttct gtgaaccact gacgatgacc
     8461 ttgttgttta cggctgtgtt tacgacgacg gaacttaacg attttaactt tctcgccacg
     8521 accttgtgct acaacttcag ccactacttt tgcacctgct acaaccggtg caccgatttt
     8581 aacatcttca ccgttaacga ccattaacac tgagtcaaat tcaactttct caccagttgc
     8641 aagttcaagt ttttctaaac gaactacttg accttcactt actcggtgtt gtttgccgcc
     8701 actttggaaa actgcgtaca tatttactcc gctatgtgcc aactgctttt gcttttaggc
     8761 actaaattaa ttcataaaaa tagggttgca attctacgga aaaagattcg aaagagcaag
     8821 agcgattttg agaatttttt gaaatttgca aaaaatggta aaaatactac cgcttgtttc
     8881 aactgcgact aaaaaacaat agactgctcc gattttacaa caaaatcata attctttttt
     8941 gaacacaggc taaattaagg tagaatctac tcaattttcg tcttacttta tggattcgat
     9001 ggctgtgcaa cttttttctt tagaacaaat tcaagccctt gcgaatgaag atatgcaagc
     9061 ggtaaatgcc gaaattttag ctcaacttaa ttccgaagtg ctactgatta accagctcgg
     9121 acattatatc atcgcaggcg gtgggaaacg tattcgcccg ttaattgcgg tgcttgcggc
     9181 tcgtgccttg ggttatcgtg gtaaaaagca tatcacggcg gctgctttta ttgagtttgt
     9241 gcatactgct acacttttgc acgatgatgt agtcgatgaa tccgatatgc gtcgtggcag
     9301 accaaccgca aacgcccatt ttggcaatgc ggctagtgta ttggtagggg atttcatcta
     9361 tacccgttcg ttccagatga tgacggattt agactctctg cgtgtgttaa aagtgatgtc
     9421 tgccgccacc aatgcgttgg cagaagggga agttcagcaa ttaatgaacg tgaacgaccc
     9481 aaacaccact gaagcgaact atatgcgggt catttacaat aaaaccgccc gcttgtttga
     9541 agccgccact caatgcgtgg cgattgcggc tgatgcttct gaggaaattg aaaccgcatt
     9601 aaaaaattac ggttgctact taggcacggc attccagctg attgacgata ttttagacta
     9661 ctccgctcaa agcgaaagtt taggcaaaaa cgtaggcgat gactttgtgg aaggtaagcc
     9721 aaccttgcca ttgctccacg caatgcacgc tgccgacaaa gcaggcaaaa ctactcaagc
     9781 agagctgatt cgcaatgcta tcgaaaacgg cggcgaattg gaacaaattc cacaagtatt
     9841 ggcaattatg caagaacatc aatctctgga ttatgccatg caaaaagccc gtgaagaagc
     9901 ccaaaaagcg gtggattcat taggggtttt accggaaagc gagtacaaac aagccttggt
     9961 ttcattggcg tatttatcgg tggatcgctc ttattaattt gcaaaaaatc atataaattt
    10021 aaccacttgt aggggcaaat gatatttgcc ccataaccta taattctttt gggcaaatgt
    10081 tatttgcccc aacataacaa tgactgaaca aaaccaacga ccccaagaac atattgaaag
    10141 cgtacgagcg agacgccgtg ctcaaaatca gaaaaaaggc ccgaatgccc cgtttgtgcg
    10201 tgagaaatta acgctgccaa acggacataa caaattactg ctgcactctt gctgtgcccc
    10261 ttgctcgggt gaggtaatgg aggctattca cgcctccggc attgagttta cgatctattt
    10321 ttataacccg aacattcacc cgctgaaaga gtatttgatc cgcaaggaag agaatattcg
    10381 ctttgccgaa aagtggggta ttccgtttat cgatttagac gatgactacg aaaacgaccg
    10441 caaagagtgg ttcaaaaaag ccaaaggaat ggaatgggag ccggaacgag gcattcgttg
    10501 cacaatgtgc tttgatatgc ggtttgaaaa agcagcggaa tacgctcaca acaacggttt
    10561 ccctgtattt accagttgct taggcatttc ccgatggaaa gatatgaacc aaatcaacga
    10621 ctgtggtcac cgtgcagcag ccaaatttga tgacgtggtt tactgggact acaactggcg
    10681 taaaggcggc ggctcacaac gaatgatcga aatcagcaaa cgtgaacgtt tctaccagca
    10741 agaatactgc ggttgcgtct attcgttaag agatagcaac aaatggcggt tggaaacagg
    10801 gcgtcagcgg attgaaatcg gtaagctcta ttactcaccc gattaaatgt tacttttctt
    10861 tgcttcgcca aagaaaagta accaaaagaa aggcgacccg aacttcgctt agttacgatt
    10921 tccttaacgg aaaatttgta aactcgctac gctcaaacag tacaaatttt cctagaaatc
    10981 gcaagtcgct cagacgggac aagcggtaca ttattcaaaa ttttttacaa aatatagtga
    11041 tgtactcttc ccttctgaac cgcccgaacg tgtttgaatt ggtaggaaaa tttactctga
    11101 gcgaagcgaa tgtaaatttt ccgtgaaacc aattcaaact aagtgaggat aagcggtgaa
    11161 gtaggggtgt ttctttggtt actttctttg cacaagcaaa gaaagtaacg aaaactcaga
    11221 tatagatttc aacttaaaaa tttacagaag gaataataac aaatgtcaaa attcgctatg
    11281 gttttccccg gtcaaggctc acaagcggtt ggaatgcttg ccgaccttgc gccacaatat
    11341 ccaatcgtgg aagaaacctt caaacaagca agtgatgtgc ttggctatga cttatgggat
    11401 ttagtgcaaa acggtactgc cgaagagtta gggcaaaccc accgcactca acctgcgttg
    11461 cttgcctcaa gcgtggcact tttccgcatt tggcaacaaa aattccctga gcaaaaacca
    11521 gccgtgatgg cagggcatag tttgggtgaa tactcagcgt tggtgtgtgc cggtgtgttg
    11581 gatttccaag atgcgattaa attggtcgag ttgcgtggtc gtgcaatgca agaagccgtg
    11641 ccggcaggca gcggtgcgat gtttgcgatt atcggtttag ataacgaaag cattattaaa
    11701 gcgtgcgagc aagcagcaag cgagaccggt gaaattgtct ctgcggtaaa ctttaacagc
    11761 ccggggcaag ttgtgattgc cggcacaaaa gcagcggcag aaaaagcagg agaattgtgt
    11821 aaagccgcag gtgcaaaacg tgcgttacca ttagcggtga gcgtaccttc acactgtgct
    11881 ttaatgaaac cggcggcaga aaaattagcc gaagccttgc aaaatattga gctaaatcaa
    11941 ccgcttgtcc ctgttatcaa caacgtggat gtggcagtgg aaacagatgc cgataccatt
    12001 cgtaatgcac ttgttcgcca actttacagc cctgttcgtt ggacggaaac cgttgaaaaa
    12061 atggcacaag atggcatcac tactttatat gaaatcgggc caaacaaagt cctaaccggt
    12121 ttagcaggtc gtattgtaaa agaattatca gccaaagcgg taaatgatgt tgccagcttt
    12181 gaggcggttg aggcttaatt ttcgggagcg taaaatgagt aaagaaaccc tatttatgct
    12241 aataagtgca ttgattgcct tagtgatttt acctttagct tatactgttg gtatcgctaa
    12301 agaaacatta ggcggaaata tgggaatgtt atccattgct gccggttgct catttttagc
    12361 ctatttttta agtctaaacc cgaaaattcg ccacgccttt atcggaggtt cagctattac
    12421 agctgctgct attcaagctt gggtattata tggtgtgcta ttaagcgaaa atgaccccaa
    12481 aagtgcggtc gttttatttc aaatttatct gtttagtagt ttagggccac tgtttttttc
    12541 agctatttgg gcatttactc gcccacgttg gatttaccct aaacaaggta gtgcggtgaa
    12601 atatgcctta tttggtggct taacgaccgt gataaactac ttgattaccc tttttatttt
    12661 ttatattctg acgttataac gtcatactaa ggagaattta tgagcaaaat cgcattagtt
    12721 acaggggcaa cccgtggaat cggcaaagcg attgccgaag aattggtagc aaaaggctac
    12781 atcgtagtcg gcaccgcaac ctctgaaaaa ggggcggaat ctatttcagc ttatttaggc
    12841 gagaacggca aaggcttagt gctaaatgtt gccgatgcag agtcgattga gagcgtatta
    12901 aagcagatca aagagacctt tggcgatgtg gacgtgttag tcaataacgc cggcattacc
    12961 cgtgacggct tattaatgcg tatgaaagat gaggattggt tcgacatcat tcaaactaac
    13021 ttaacttcgg tttatcgttt atccaaagcg gtattacgcc cgatgatgaa aaaaggcgga
    13081 cgcattatca cgatcggttc tgtggtcggc tcaatgggca acccggggca aaccaactac
    13141 tgtgctgcca aagcaggctt agttggtttt tctaaatctt tagcgaaaga agtagcaagc
    13201 cgcggcatta cggtaaacgt ggttgctccg ggctttatcg ccactgatat gaccgatgag
    13261 ctaaccgaag atcagaaaaa tgcgatttta agccaaattc ctgccggtga actcggttct
    13321 ccacaagata ttgccaaagc agtcgctttc ttagccagcg atgatgcccg ttatatcaac
    13381 ggtgaaacca ttcacgttaa tggcggttta tatatgagct aatccgctac aagcggtctt
    13441 ttttcaaccg agctttgtaa attcagagtt cggttttgtt atttgttatt ttgtgttatc
    13501 acctgcttta taggtatagg tgaaaaagtg tgaatatgtt atgactagaa atacattagt
    13561 agtaggcttt ttattgtttg ctattttctt tggtgccggc aatctgattt tcccaccaaa
    13621 attaggttat gaaagtagta cagaattttt ctcagccatt tcaggctttg ttttaactgg
    13681 agtagggctt cctctactcg gtattatcgt cagtgcattt tataatggag gctataaatc
    13741 tgcattagac aaaatccacc cttggttttc tttagcattc ctaacttcta tctatttagc
    13801 gatcggtcca ttctttgccg gtccgcgcac ggcagcaact gcctatgaaa tggcgataat
    13861 gccgttttta ggggattcca gccaagtgtc aatgttaatt tttaccttaa tttactttgc
    13921 ctttacccta tggttagcgc taaatccaaa taaaatggtt gatcgcattg gtgcaattct
    13981 taccccgatc ttgcttataa ccatcattgg attagttatt cgagccggct ttattttaga
    14041 taccgatcca caaatcacta cgcctaatac gaatgaacaa tcttactttt ttaaaggtat
    14101 ggttgatggc tatctaacta tggatgcact tgcttcgtta gcctattcgg taattgtatt
    14161 aaatgctatt cgtgggaaag gggtaagcca agccgcatta caacgccaaa cagtttctgc
    14221 cggcttagtt gcagcagttg ctctagctgt aatttatatt tcattgggtt ggattggtaa
    14281 taatatgcca attagtgcaa acacactttc gatgttatca gaaagaaaat tggatctagg
    14341 tacttacatt cttaacactg tgactaccca agccttcggt gaactcggac gtagtgtctt
    14401 aggagtgatt gtttcacttg cctgtttaac cactgctgtc ggtttagccg tatcagtcag
    14461 tgaatacttt aatgaaattt tcccgaaaat ttcatataaa gtttatgcta tcttatttac
    14521 tgtgattagt tttactgttg ctaaccaagg attaaaagaa gttattagca aatccattcc
    14581 ggtattatta gttctctacc caatagcaat gactgtcatt ttaatgttgc tgattaattt
    14641 agtgattaaa cttccgttgt ctgcacaacg tctatcactc ctattagtga caattgtagc
    14701 gatagcttca gttgccggtg tcgggtttac cgaaaacttg cctctaaaat cctactcgat
    14761 ggaatggcta cctttcgcta tcattggagc tattattgga gcaattatcc acccgcttac
    14821 cggtcgaaag cataaataaa gtaaaggcag tgagcattca ctgcctcttt tttaggagag
    14881 aataatgcct gaattacccg aagttgaaac cagcgttcgt ggtgtatcgc cctatttggt
    14941 tgggcaaact attgaacaaa tcatcgttcg tcagccaaag ttacgttgga cggtgagtcc
    15001 tgaattaagc caaatggcag gggcaaaaat taccgcagta gaaagacgtg ccaaatatct
    15061 gattatccgc accgacaaag gcgatatttt ggtgcatctg ggaatgtccg gctcgctcgg
    15121 gattttgcaa gaacgtcaac aaaaagaaat tggcaaacac gaccacgtcg atcttattac
    15181 ccaaaacggc acaattttac gctataacga ccctcgcaaa ttcggctgtt ggctatgggc
    15241 agaaaaagca gaagatcacg aactgttgcg taaactcggg cctgaaccgc tttcaaacga
    15301 tttcacagca gattacttat ttgcaaaaag ccgcaaaaaa acgaccgctt gtaaaacctt
    15361 tattatgacc aatgaagtgg ttgttggggt ggggaatatc tatgcgtgcg aaagtttatt
    15421 tatggcaggc attcaccctg agcttgcagc acaaaacctg actaaaaaac agtgcgaacg
    15481 tttagtaaaa accatcaaag aagtgctgac taaagcgatt attcaaggcg gcacaacact
    15541 gaaagatttt atccaacctg acggcaaacc aggctatttt gctcaagtgt tgcaagtgta
    15601 tggaagaaaa ggggaaacct gtaatgactg cggaaagccg attgaaacta aagtaatcgg
    15661 gcaacgtaat acctattttt gcccgaagtg tcagagattg ccgaagtaat ctaataataa
    15721 actctctccc cctgagggag agagacagct tgttgaaacg ttaagtgaaa caagcagaga
    15781 gagggtaaaa cctaaacctg caaccaaaag gtaacaggcc cgtcattttg taggctgact
    15841 tgcatatcgg ctgcaaattg cccggtttgg gtatgaatct tagtggcggc ttgttgatga
    15901 aagtaggcat acaaacgttc ggcatcaacc gggcttgctc cttttgagaa actaggacgc
    15961 aagcctttat tggtatccgc cgcaagggta aactgtgaaa ccactaacaa actgccacct
    16021 gcttgctgta cgtttaagtt cattttcccc tcgctatccg aaaacacccg ataattcagc
    16081 actttttcca gtagcttatc tgctttcgct tcatcatcgt ctttttccac accgagcagc
    16141 accaacaaac cgcttgcaat ttcgccaacg acttgccctt caacctccac ttttgcgaat
    16201 ttaacccgct gaattaagcc tatcattaat gctccccttg tctgtttcca tttcgtctaa
    16261 aaccgccgca aattgcccgc caagtaatac cacctgccaa cttaactgaa tccataacag
    16321 cataatcggt aacaccgcta atgctccata aattgcctga taagacggga aggcagtaat
    16381 ataccaaata aaggctttct tccctaaagt aaagaaaatc gctgccatta atgccccgat
    16441 agcagcgtgt tgaaatttta cctttctaat tggaataaat ttatacacta aggtaaaaag
    16501 tagccaaatt agcaaaaacg gcatcaattc taataaatgc tgcccgagcg ataacgctcc
    16561 atttccatta aaaatagaaa gcgacattac ataggtgcta atcgcaatgc tagcaccggc
    16621 aagcagcggg ccaaacagca aaatgagaaa gtaaagcaag aaagagaacc acatcggacg
    16681 ctgacgatta tcgtgccaaa tggtattcaa tgcctcatca atactcttaa tcagcataat
    16741 agtaaccaca aataagccga taaaacttac cagccccatt tgtttggcat tactgacaaa
    16801 caactcaatg tattccgaca ccatctcgct cgcactcggg gcaaagttgt cataaatgaa
    16861 cgcttttaac tgctcgtttg cttcgtggaa aatcggaaaa aaggtaaaaa tggaaaacag
    16921 cacgacaata aacggcacta tcgccagcat tgtgctataa gtgagataac cggctgaaat
    16981 cgcaatgtta ttttcattgt aacgcttggc aaagagctta ataaatctcc acagtcgatt
    17041 taacatctta acctctcacc aaataatcgc cttcacacac ccatttgtag gtggttaagg
    17101 cttctaatcc catcgggcct cgtgcgtgta atttctgcgt gctaactgcc acttctgccc
    17161 ctaaaccaaa ctcaccacca tcggtaaaac gggtgctggc attaatataa accgccgcgg
    17221 catcaacctg ttgtacaaat tgacgggcaa gttgataatc ggaggttaaa atcccctctg
    17281 aatgctgcga accataatgg cggatatgct ctaccgcttg ttgcaggtca ttcaccacca
    17341 ccacgttaag atcaagcgat aaccactctt ggctcaattt tgcttgatca agcggttcaa
    17401 ttccttcaat tttttgcaaa tcgttactgt gaatagtaac gccaagctgt tgcattttgg
    17461 cgaccaattt cggcaaaaat tccaccgcaa tcgctttttc caccaacagc gtttccagcg
    17521 tattgcaagt gcttgggcgt tgcgttttgg cattagcgat gacctctaga gatttctcct
    17581 gatccgctgt tttttccaca aacagatgac aaaccccaat cccccccaca atcaccggaa
    17641 tggtcgaatt ttctttacag aattgatgta aacctgcacc gcctcgaggg atcaccatat
    17701 caatgtagcg atctagcttc aatagttcca ataataaggc tcgatccgga tccgtcaccg
    17761 cttgcactgc caatttcggc aagccggctt tttctaacgc atcttgcact acatcaacca
    17821 acacagcatt ggtaaatttg gtctctttac cgccacgcaa aatcactgca ttgccggttt
    17881 ttaggcataa tgatgccaca tcaatggtca cattcgggcg agcttcgtaa atggttaaaa
    17941 tcaccccgag cggcacacgc tggcgttcga ttttcaaacc gctgtctaac acgccaccgt
    18001 ccatcacctg ccccaccgga tccgctaatt tggcaacatt tcgcacatcg ttagcaatgt
    18061 cttttagccg atcttcattc aataataagc ggtcaataat cgccaatgaa atcccttgtg
    18121 cttgagcaaa ttcaatatct ttggcattag cgtataaaat ttcttctgca cgttgctcaa
    18181 ggctatcggc aatcgtgagt aaggcttggt ttttctgtag gttgccaaat tgagccagct
    18241 caaaactagc ttgtttggct tgctgggcaa gttctatcat ctgcataaat taggtctcat
    18301 ttaatcaata ttctccataa tctaacacgt tcttttatgt gaagaaatat gcaaaagcca
    18361 agaaaattgg ataaaatggg ggaattttaa aacattacgc aattcccctc tccctgattt
    18421 ttctcctgaa cggagaaaaa tctatccctc tcctacaagg ggagagggta gaatttaact
    18481 ctctcccctt gtgggagaga gaccgcctac agattcgttc agaatctgta ggcagagaga
    18541 ggggtaaaaa cagataaaac ttaaacaaaa tgataaacac tacccctaaa cagctaatcc
    18601 acattcttcc gcctcaactg gcaaaccaaa ttgctgccgg cgaggtggtg gaacgccctg
    18661 cgtctgtggt aaaggagcta gtggaaaaca gtttagatgc cggagccaat actattcaaa
    18721 tcgacattga aaaaggcggc tctcagctta tccgcattcg agataacggt tgtggtatca
    18781 gcaagcaaga tttaccgctg gcacttgctc gccacgctac gagcaaaatc agcagcttgg
    18841 aagatttaga agccatttta agtttaggct ttcggggcga agcattggca agtatcagct
    18901 cggtttcacg tttaacactg acctctcgta ccgcagagca aaccgaagca tggcaagcct
    18961 atgcacaagg gcgggaaatg gcggtggaaa ttcagcctgc ctcacacccg attggcacaa
    19021 ccattgaagt agcaaatctg ttttttaaca caccggcacg gcgtaaattt ctacgcaccg
    19081 ataaaaccga atttgcccat attgacgagg tggttcgccg cattgccctt gccaagccga
    19141 atatcagttt cagcttaagc cataatggta aaatcgtccg ccaatatcgc aaagtgcaag
    19201 acaattcggt agagcagcaa caaaaacggg ttgcagccat ttgcggtgat gagtttattc
    19261 aacattcaac ttatattgac aggcaacacg gggatctgca cctacacggc tgggttggct
    19321 caccggctct tgcccgtctg caaaacgacc tttgctacag ctatgtgaac gggcgaatga
    19381 tgcgggataa aaccatcaat cacgccattc gccaagccta cggcgacagc attgcaagtg
    19441 gttactatcc ggcttttgtg atctttttgg atttagatcc gacaatggtt gatgtaaacg
    19501 ttcacccggc taaacacgag gtgcgctttc atcaaggaag actggtacac gactttattt
    19561 tgcaaggcgt actcaacagc ctgcaacaaa cccaacctac gcttgaactg cgaaatgaag
    19621 tgaacgagcc gttaccgagc tatgttcaag acactaaccg ccaagctgcg gggcaaaata
    19681 tttttgtgtc gcaaaccgct ccacaagcgg ccgaatttac gcaaaaaaat gcaagtcgat
    19741 caagctatac gcctaaattt gaacgaagca acagtgtgag ttcgtccgct caaaaatggt
    19801 ataaggaatt ggttggtggc gatttgcacc aaacaacgcc aactatcaaa acgcaaagca
    19861 cgcttgttga gccaaaaaca gaaccccaaa ctgaacataa aacagaagcc gtttcgcaaa
    19921 aaattacgca aattgcaccg cttgcaagcc accattcaca agccttagcg attgtaaaaa
    19981 accaagcggt gttaattaaa gaagaggaaa atttttacct gcttccactg gcaacattag
    20041 ccgagcttaa actcaagcta caactggaaa aagggcaatc tcaagcgttg ttgatcccac
    20101 tgaatctcag tttagatcaa caacaaaccg aacgctggca acaaaccaaa gaagaactaa
    20161 ctaagcttgg cttttcgatc agcgaaaaac agtggcaagc ccaaacacgg ctcacagtgc
    20221 tttccgtacc acaggctgtc agagaacaaa acctgcaaca gcttttattt gctctgttca
    20281 accaaacaca agcggtcaat ttagccgaat tttttgcaaa aaaatgcgaa aaacccaccg
    20341 cttgtacgct aagtgacgtg attgctctac tctcagaaat tgagctttat gccaatggga
    20401 aaaaagagct ggagggaata aggaaggaag tggatttttc caagttttta tagtctaaag
    20461 ggcaataccc tttagccaaa taaatgagag tggctgccaa ttcgcagcag ctctagctta
    20521 tcatcttcca cttttcgata gataagaacc aaatcaggtt ggatatggca atccctaaag
    20581 cctttccatt cgccggttaa agcgtgatcg cagtatttct caggtaatgg cttgtcatta
    20641 atcagaaaat acaacgcctc aattaatgaa gaggataaag actcaaccgc acatttttta
    20701 tagtcacgtt taaaagcatt actatactca atctctcgca ttgagttgct ccattaaatc
    20761 cgcaatacta tttgctgaat gtagcttttc ctcgccattc ttaacagccc ttaatacttc
    20821 tgctgtctca aaattaggct gataatcaaa ctgaaaacgt ttttctgttg cgattttcgc
    20881 taatgtcatt cgcatcacat cagagacagt caagccgata ccggctaaca cctcacttgc
    20941 ttgctcttta atttcagggc tgattcttac gcttactaat gcactatttg ccatcaaaaa
    21001 ctccttttgt atatcaaatg atatacaaaa taacatttaa caaaaaattg tgcaataaaa
    21061 aagaaattca aagaaaagaa atgaatagtt aaaattagct cgttacaaga aatcattgag
    21121 ttactcaaga aatcaaggta atataaagaa gtctgggcgg aacacgaacc cgcccctagc
    21181 atctagccgc ttacaagaac agatagatta gttgaaagag cgtgaaaatt tcaactgctg
    21241 cacacagaaa cccaagtgca actaaaatca atagtagttc tttcattgta agcctcctta
    21301 gtgctactaa ggatgattta ggctcaggct actgcgaata gccctaacga gcctaccagt
    21361 agcacaatca ggattgtaac aaaaaagctg tgaatttcac ggcttttttt gtatctatat
    21421 acagtatatt aatatgaata aaaaacccct tgcccttttt ttaatggggc cgactgcctc
    21481 cgggaaaacc gatttagcta ttgctttacg ccaagaattg ccggttgagg taatcagtgt
    21541 tgattctgcc ttaatttaca aaggaatgga tattggcacg gcaaagccaa gcaaggaaga
    21601 gcttgccctt gcaccgcatc gcttgattga tattttagac cctgccgaga gctattcggc
    21661 gatgaatttc cacgctgatg cgttgcgtga aatggcagat attacctcac agggtaaaat
    21721 tccgctcttg gtgggtggca caatgcttta ttacaaggca ttgcttgaag ggttatctcc
    21781 cctgccctct gccgatccag ccattcgggc tgaaatcgaa agccgagcgg cacaacacgg
    21841 ctgggcaaca ttgcatcaag aattagtgaa aatcgacccg attgccggtg caagaattaa
    21901 cccaaatgac agccaacgga tcaaccgagc gttagaagtt ttttacatta ccggcaaaac
    21961 gatgaccgaa ctcactgctc agcaaggtga ggctctacct tataatatct tacaatttgc
    22021 aatcgctcct caagatcgtg cagttctaca ccaacggatt gaacaacgct ttcacaaaat
    22081 gattgagcta ggctttgaag aggaggtcaa gcggttattt ttacgcaaag atttgcatat
    22141 taatttgccc tcgattcgct gtgtgggcta ccgccaaatg tgggaatatt tgcaaggcga
    22201 tatttcgctc gatgaggcga tctttaaagg catttgtgcc acccgccagc tggcaaaacg
    22261 gcagattact tggctgcgtg gctggcaagg cgaactgact tggttagata gtttagctcc
    22321 gaccagttct aaagcaaaaa tggtggaaaa gatcgatcat tatttaaaga attgtgataa
    22381 tatctaaacg cctttggcac ttatcgcatt atttggtgtc ttaaaaatca cacaactata
    22441 aaaaggaaaa taacaatggc aaaaggtcaa tctttacaag atccatattt gaatgcactt
    22501 cgtcgtgagc gtattcctgt ttcaatttat ttagttaacg gcattaaatt acaaggtcag
    22561 atcgagtcgt tcgaccaatt tgtgatttta ttaaaaaaca ctgtaagcca aatggtgtat
    22621 aaacacgcta tttcaacggt tgttcctgcc cgtgcgttat cgcataacaa taacaaccaa
    22681 gcccaacacg ctcagcctgc tgccgaagta actgaacaag cggaataata acattattac
    22741 tcaaccaatg ttaaacgaat cattgccaaa ctcagaagaa aattctgcgt ttggctttgc
    22801 tttatctgaa gcaagccaaa ctacagaagc cgcctcgcct accgatccag aaaatcaaga
    22861 taaagccatt ttggtgcatc tctatctcgg gcaacataaa gatgttgaaa atttgctgga
    22921 atttaaaacg ctggcagaat ctgccggtgt cgaagtgctg gcaacgctta ccaccagccg
    22981 ccaaagttcg catatcaaat attatgtggg gcaaggcaaa gcggagggaa ttgccgaagc
    23041 ggtagaacga ctcggggcaa cagtagtgct ggtgaaccat caacttagcc cggctcagac
    23101 ccgtaatttg caatcgttat gcggctgccg tgtggtggat agaaccggct tgattttgga
    23161 tattttcgct caacgggcaa gatcgcacga aggcaaacta caagtcgagc tggcacaact
    23221 tcgccattta tcgacccgtt tggttcgccg agttactaat caagaccaac aaaaaggcgg
    23281 agcagttggc ttgcgtggtc caggtgaaac acagctcgaa accgatcgcc gtttgattaa
    23341 agtgcgaatc caacagttac tcaaccgctt ggaaaaagtc agcaagcaac gcaatcaaaa
    23401 tcgaaaaacc agacaaaaag ccgatattcc aacagtatcc ttagtgggct ataccaatgc
    23461 cggcaaatct accctgttta atgcgattac ccaagctagt gtttatgctg ccgatcaact
    23521 ttttgcgacc cttgacccga ctttacgccg gattcaggtg caagatgtcg gcacgacaat
    23581 tcttgccgat acggtaggtt ttatccgttt tctaccgcac gatttagttt cagcctttaa
    23641 atctaccctg caagaaacca ccgaagctac tttactgctc cacgtcattg acggctcaga
    23701 cgaacgcaaa aacgaaaata ttgatgcggt aaatcaagtg ttggacgaaa tcggagcgtt
    23761 agatattcct actttattgg tattcaataa aattgataaa ttagaaggtg tagaaccgca
    23821 tattgaacgc aatgccgatg gcacgccagt tgccgtgtat ctttccgccc aagaaaatag
    23881 cggcattgag ctgttattcc aagccattca agaaaggctt cgcagtaact tagtgtgtga
    23941 aaacttgctc ctcccggtta ctgccggtgc aatttacgca caatttaaag cagaaaactg
    24001 cattaaaacc gagcatttta atgagttcgg tgaacggatt atctctattg aaattaacga
    24061 agtgcaatgg aacaaatggg tgaaacaatt tccggatcta gccgaatata ttgagctggc
    24121 aaaatggggg taattaatct cacttctgag taagttatat ttaaattatg caagcggtca
    24181 attctttacg tttttttgca aaaaagtgta aagaattgac cgcttgtttt atccattaaa
    24241 tccaatttag ccaataaaaa aatccctcaa ataaaccaga tttccccgtt ctatttgaag
    24301 gattttattt attcttcccc aaaagtgggg aagaaaagct taatcaagca gttactacca
    24361 ctgataacct acgcttgcag cagcacctgt atcaccacgg ctgttagagt taacactgaa
    24421 tttgataccc actttaccat tatctgataa acgagaataa cctacagcaa tagctccttg
    24481 accacggtaa gcacctacac cggctgaaac catcgattta ccaggttgtg ttacgtgata
    24541 taaattacct gcggctaatg cacctgcaat acctgcacgt aagtctttat ctacgttact
    24601 gatacgattg ttcagacgat tttctgcacc tttcaactga ttcacattaa cagcatcagt
    24661 gcctttcaca cccggagcaa cgttcgtaat acgtgtttgc gcattcttgc tacctgcaat
    24721 gttaagctca tttacgccat cttttgcaac agttgaactg atagttgtac caccaacttt
    24781 cactgaatca aattcaagat tgcttgaggt tgccacttca acagttgaac ctttacgtga
    24841 aacaacgatg tttttgccac ctgaaacggc cactttacca ttgttattaa cacgttgagc
    24901 acctttctct gaagtttcag ctacattacc atcaacagtg agataccagc ctgtattctg
    24961 cattgcatta tacaactggc taccgctcac aacatcttta cttgttgccg ttacatcacc
    25021 atctttaacg ttagtgatct tcgtgccttt cgcatcaata ccgcctttag ttacacttgg
    25081 gccgtcttta atggttaaac cattgttatt tacggttgtg tcgccagttg ttacagaacc
    25141 atcttccgtt aaatcaattg ctttcgctaa ttgtacctct aatgtgtctg taccattcgc
    25201 tttcacattg atgttgcctg atgctgactt aaagttttca gaagctgttt tatccacacc
    25261 ttcacctttc actttaactt cagtattcag tttctgacgg ttcttcacat cttcattgtt
    25321 accagagaag attaaaccat catttaaggt tgcaacttgt tctgtgacag ttttaccgtc
    25381 tttatctttg gtctcataaa cgatacgagt cttaccgttc tcacccggta atccatcttt
    25441 accatttgta ccatcaacac cttcagcacc atctttcact gcaatatttg cacttgcacc
    25501 atctttaccg tcagcgcctt tcggaccggt taagccgata gagccatctt taccgtttaa
    25561 tacaacagaa gcgccatctt tacctgtagc tccaattgaa ccatcaacac cgtctttacc
    25621 gtcacggcct gcagtaattg tgccgtcacc tttaatggtt acgccatctt taccgtcttt
    25681 gccagcaata ccgatttgac cgtctttacc atctttacca ccaatggtta agttgttttt
    25741 caagccgaag gtcacgttat taccttcttt agtaataata atgttaccgt ctttgttgtt
    25801 tagatcaact gttgctcctg gtttaacgtt gcctttgtct ttaccatttg ctgttaagtt
    25861 ccaacctgcg ttagcaactt cttgcacttg atataactgg ctaccattta ccgcatcttt
    25921 gctggttgca gaaatttcac cgtcttgaac gttaatgatt ttcttatcgc ctgcattgat
    25981 accggcttta gttacacttg ggccatcttt gatagttaaa ccatcattat ttaccgtagt
    26041 atcaccaata gttacagaac catctttcgt taaatcaatg tctttcgcta atttaactgt
    26101 taacgcacca ttgttgttta caacaccgat attgttatcg gacaggttcg ttaaatcagt
    26161 atcaccgcct gagattttta acgtctcatt aagtttctta acgatttctt taccgtcatc
    26221 acctacgaat cttaagccat cgttcagtgt tgcaacttcc tcagttgtac cgtctggttt
    26281 ctcataaacg atacgggttt tgctttcgcc atctttaccg tcgttaccgt caaggccttt
    26341 agcaccgtct tttacagaga tagttgcact tgcaccatct ttaccgtcag cgcctttcgg
    26401 accggttaag ccgatagagc catctttacc gtttaaagta acaccaactg taccatctgc
    26461 acctttaaca ccgattgaac catctttacc tggaccgcca atggtaaaat cgttatttaa
    26521 cgtaaagtca atcacattat cacttactgt tgtgatgatg tttttgtctt tagaggtgta
    26581 cttaacagtt tcacctagtt taacgtgatc agtttcacca ttatccgctg caatgctaaa
    26641 gcctttctcg ataatatcgt tgcttgcatt tacaacatca ttaatattcg cagcgttagt
    26701 accaacttct tttaattgat cgtctgttaa gtcgttcact gaagtgattg gcttaccatc
    26761 tttaccgtta atgccgtctt tacctgctaa accgtcaata ccgctcttca cgttaacaat
    26821 ttggttgccg ccattgtcta gaccgtcttc agtaagctta actgctttac catctgtgcc
    26881 atcttttggc gtgatagtta cgccattatt atttactact gttgttggac ctttaccttc
    26941 ttgaccattc tctccatctt gtggtttgaa tgtagctgag ttcaagtctt taagatcttt
    27001 agctaattga acagataatt taccatcttt gctgtttacg ccgatgttat ttgcagttag
    27061 cttatcttta tcagctccac caatgatttc taaggtttca ttcaatttct tagatactag
    27121 tgttgcatca tcacctttaa agtgtaaacc atcatttaaa gtagctactt gctctggttc
    27181 acctatagga tcaccattgc catctgttgg ctgatattgg atacgagtta caccatcttt
    27241 acctggtaaa ccatctttac caacaagccc tggtttgccc tcagtacctt taatggttaa
    27301 accatcttta ccatttgtac cgttcaagcc gattgaacca tctttaccgt taagaacaac
    27361 tgcagaaccg tctttaccgt ttacaccgat agtaccatca ataccatctt tgccagggcg
    27421 acctacagtg atagctccat ctttaccatc taaagcaata gcatctttac cgtctttacc
    27481 agcaatacca actttacctg gttcaccatc tttaccaaca gtaatattat tcactaggct
    27541 aacttctaat gcatggccac catctttctc aacaacgttt gttttaacat tgccatcacc
    27601 aatgatctta gctgtcgtac ctaagttttg cttaaactcc ttgttgttct catcttttag
    27661 accaaacgca tctgttgtca cctcagtaac agtctcagta acagtatttt tcaagtcacc
    27721 gacgttaacc gcattgttaa gaacatcacc tgctgcctca gtaagtttaa caggttgacc
    27781 atctttacct tttaagccgc tatcaacatt ggtgatttgg ttaccaccgt tgcttaagcc
    27841 atcctctgtt aaggtaacat tagatttagt tgcatcatta ggatctttct tagcaatcgt
    27901 tacaccatct ttattgataa ctgtagaatc accttgtgtg ccgaatgttg cactatctaa
    27961 gcctgctaga tcttttgcta acgccacagt aatggtattg ccaacaacct gtgtcatgat
    28021 atttttacca gcatcaaact tagcccaatc agtgtttgtt gtagcacctt taatatccac
    28081 ctgcgtattt aatttcgcat cgtgaacttt gccatcattc gcaccgaatt ttaagccatc
    28141 gttaagtgtt gcaacttctt ctttatcacc atttggtttt tcgtaaacaa tacgagtttt
    28201 gctttcgccg tctttaccgt cgttgccgtc aagaccttta gcacctttct caactttaat
    28261 tgatgcttta ccatcttttc catcttttcc atttacgcca tcaacgccgt ggaatacgat
    28321 ggcttctggt ttaattgtta caccatcttt accatcagca cctttaacac caactgaacc
    28381 atctttacct ggtttaccac caacagagat gttatcggat aagccaaaat cgatggtatt
    28441 atttgttacg gttgtaacaa cattcttatc ggttgaggtg tacttaacag tttcacctaa
    28501 cttcacattg tcttctttag caccacctcc taagccagag tgatccgctg taatgttaaa
    28561 gccagtttta actaaagtgt cagctactgc gtaaagttgt gaaccgttaa tcgcatctgt
    28621 tgaattggaa gcaattttac ccggtgcaac aaacttaatt tggcgagcat tggttgcatt
    28681 acccacactc acataatcac catcaacaac attttcatcg ccagcaaagc tgctatattt
    28741 taatgtgcca acagttgcag tatcttcttt agttgcggtt gcttcagtag agtcagaacc
    28801 tagaattaca gaaccctttg tcttaacagt aacattagaa cctaatacga aagtatcttt
    28861 acctgaaact gtattgttat aacctaaagc cgcagcacga atacccgaaa cagtactatt
    28921 tacaccggct gcaattgagt ttttaccctt tgcggttgag ttgtaagcaa tagcagttgc
    28981 ataattacct tttgatttgg cctcaaaacc aattgctgtg ccaccttcat gcgtaatatt
    29041 ggcacgtgta cctactgctg tcgcattcgc cttacttaca ttagcactat aacctatcgc
    29101 aacagaatta ttaccacttg aattagccgt atcaccaatt gccattgcac catctttgct
    29161 cgcgttagaa tgcgaaccaa tactgatcac tttttctgca tgagagtttg cattccaacc
    29221 aatgctaata gcattacttt tccaggcttt agaagcatta ccaaaagcaa tcgcaccgat
    29281 tcctgttgta ttcgaattta caccaaaggc gatagaacca tcacctgttg cacttgcatt
    29341 tgttgaaaac gccaaagtat cattcgaatt tgcacgagta ttcgtaccca aagcaaatgc
    29401 attatttgct actttcgctg tatcaggatt aattacctgt cctaattcag aaatatttga
    29461 attctctaat ttaaagccac cggaccaagc gttagtacca atggcaaatg aatggttacc
    29521 attagttttc gctttagtac cgattgagaa tgcatctgtt ccaaatgcta aagtattatt
    29581 ttgcccatca taaattgaac gtctattttc atgaccgtaa cttccatcca ctgtctgtgg
    29641 agtaccacta ccaatcgcaa aagaacggtc acctagcgct gtagccgaag caccaatggc
    29701 gaagctagct gtagcatttg cccaagatgt tgaaccaatt gcagtagaat aatctgcaat
    29761 tgcagcagat tgacgcattg cagcaaatga gttaaatcct tccgcacgtg cagaggtacc
    29821 taatgctaca gaaccaatac ttgcagtacg agaaaaagaa ccaattgcaa tagacaatcc
    29881 ccctccagaa tatgaataat caccaatggc tatgcctgac gctttcggtt ttcctctact
    29941 ttttctgtta ttgtctaaag ctacagcatt gtgtccaatt acaattgctg aggtagtatt
    30001 agacttatac aataatctag aatccttctc cttattaaca gagttattag attcttgaag
    30061 cttagttctg tctgcatagg ataagctacc tgggttaaag tagttatata gattcgtaat
    30121 agaatctgta gccaaactag tgtttgtata aatatctata ccaccattaa caactttttt
    30181 tggtgctttt tgttgtactt ctattctatc aataatcacc gccgcttctg ccggtgtagc
    30241 agaaagcata aaggctgcca ttccactgat aagggttgct ccccctagaa tgctagccgc
    30301 tgctaattta atagtaccgc gcttatcagt agaagaagcg gctttacctt gcgatcgagc
    30361 aagttcagac acaacttcca ttttttgcgt cgtttggtta aaaataacct taaatatttt
    30421 gttcataatt ttataccttt agaattatta taaagcttac ttcgtaaata attcggcaat
    30481 tacaacccac cgaataaaca accttactac tcttaattta gtaagaggaa agtcatatcg
    30541 ataatagtaa gattttcact tca
//
LOCUS       DANLZC010000009        29853 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.009, whole genome shotgun sequence.
ACCESSION   DANLZC010000009 DANLZC010000000
VERSION     DANLZC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 29853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29853
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.009"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(199..1074)
                     /gene="ubiA"
                     /locus_tag="PXE44_000357"
     CDS             complement(199..1074)
                     /gene="ubiA"
                     /locus_tag="PXE44_000357"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDL5587903.1"
                     /translation="MNNETLFSKQKWIAYAQLMRFDKPIGTLLLLHPTLWALFIAAEG
                     MPPVAILAIFTLGVIVMRAAGCVINDYADREIDGHVKRTSQRPLATGRVTTTEAKVIF
                     AILLLFAFLLDLMLNRYAFLLSFVAVFLAVIYPFMKRFTHLPQVVLGMAFGWSIPMAF
                     GAVTETLPLECWLLFFANLAWTVAYDTQYAMVDRDDDLRIGVKSTAILFAQYDNKIIA
                     LLQFITLILLGLLGVVKDYHIGYFVVLALSATLFIYQCWLTKKRERSECFKAFLNNHY
                     FGLGVFVAILVGIYF"
     gene            complement(1067..1828)
                     /locus_tag="PXE44_000358"
     CDS             complement(1067..1828)
                     /locus_tag="PXE44_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDL5587904.1"
                     /translation="MKQANRHKKIIELVNQLGYVSTEQLVAELNVSPQTIRRDLNEMA
                     ENNLIRRHHGGAAAPSNTENSDYTHRKQFFSHEKNAIAQQVAKLIPNGASLFLDIGTT
                     SEAVALALLSHKNLKVVTNNLNAAHILMQNEDCQITVAGGGLRSDGGLIGEETVRFIN
                     QFRLDFGILGISAVDMDGSMLDYDYHEVQVKRALMECSRQVVLVTDHSKFTRTAIARL
                     GNVKEVNYLFTDQDLPLELQTHLSQSDVIVKICNE"
     gene            complement(1895..3262)
                     /gene="purB"
                     /locus_tag="PXE44_000359"
     CDS             complement(1895..3262)
                     /gene="purB"
                     /locus_tag="PXE44_000359"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL5587905.1"
                     /translation="MELTALTALSPIDGRYQDKVASLRPIFSEFGLLKFRVTVEVRWL
                     QKLASHTQINEVPVFSEKANDYLNQIVADFSLEDANRIKTIERTTNHDVKAVEYFLKE
                     KCEALPELQAVNEFIHFACTSEDINNTSHALMLKTAREEVLLPEWKKVIDAVAELAKC
                     YQHIPLLSRTHGQPASPTTMGKEMANVAYRLQRQYKQLENLEILAKINGAVGNYNAHL
                     SAYPEIDWHTFSQEFVESLGVTWNPYTTQIEPHDYIAEFFDCVARFNTILIDFDRDMW
                     GYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAVMAHLGQKLPISRWQ
                     RDLTDSTVLRNLGVGLGYALIAYASTLKGISKLEVNEQHLRDELNQNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDETAMREFIDKLEIPENEKNRLKEMTPASYIGYAVE
                     LVDKL"
     gene            complement(3296..3925)
                     /gene="hflD"
                     /locus_tag="PXE44_000360"
     CDS             complement(3296..3925)
                     /gene="hflD"
                     /locus_tag="PXE44_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDL5587906.1"
                     /translation="MADYHDITLAYAGVCQAATLVQQFAHKGMADREAFKCALESLLI
                     TQPESTLSVFGDDLSHLKMGLETALAQTGGGNGKLDTEVGRYWISLLALSQKLNKNPE
                     AKQQLAQRLQQVERQLALYEDNVMADQMIANLAAIYSDIISPLGTKIHVMGMQDYLVR
                     PDIQNKIRASLLAGIRAGILWQQVGGSRWQFLFSRKKIFNQAQKFYRQI"
     gene            complement(4057..5721)
                     /gene="cydC"
                     /locus_tag="PXE44_000361"
     CDS             complement(4057..5721)
                     /gene="cydC"
                     /locus_tag="PXE44_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDL5587907.1"
                     /translation="MKSLFPFFALYQTHFGRLLLGVVLAILGLAASIGLLSLSGWFLA
                     ASFLAGNALIFNFFYPSSGVRGLAVGRTISRYFERLVTHDATFRVLANLRASVFKKLI
                     PLSPSGLNRFRNSELLNRLVADVDTLDTLYLNLVSPFVSAVMIIAFMAIGLSFVSVPL
                     MLIICGTLLVLLAVIPTVFYRLGLKLGRNAIQSRANYRSQFIEWVQLNAEFLLFGNLN
                     QMSEKLQQTEQQWLNAQSKESRLSGLSNSLIMLSNGILTCVVIYLVSTSINVPTAKYP
                     EALIALVIFCVMASAEILSPIGIAFLHLGQVITAAERISEITEQQPNVTFGSKAEWQN
                     LSQNQPLVRCENVSFAYYGEQNVLNNLSFKVLKGQKVAILGKTGSGKSTIFQLLNRNY
                     DPTSGQIWLNNCKIDEYSEPMLRSKLVTLSQRVHIFSQTLKDNLLMGNAQATDEQMQE
                     AVRKVGLGHLLETDGLNLWLGEGGRPLSGGEQRRLGLARLLLSSAELVLLDEPTEGLD
                     RETEQQILNLILTHCCDRTLIMITHRQSGLDKFDVVYRIDNGRLVG"
     gene            complement(5721..7487)
                     /gene="cydD"
                     /locus_tag="PXE44_000362"
     CDS             complement(5721..7487)
                     /gene="cydD"
                     /locus_tag="PXE44_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDL5587908.1"
                     /translation="MEKQRQRELQKWLKSQQKVIKKYMHLNILLGSFSSLCMIGQMWL
                     IATMLDKMLIGKQSPNSFLVEIMLLFCCFAARAFFIFLRERVGFKAGQTLRLHLRRQI
                     LAKMEAVGPMSIQQKPAGSWATLMLEQVENLHNFYARYLPQQFLSLIVPLLILCFVFP
                     INWAAGAILFATMPLLPLFMILAGMKAVEANQRNIGILSRISGQFLDKLKGLETIRLF
                     GQAEKQTEQIYQSTEDFRISTMDVLKMAFLSSAVLEFFTAVSIAVTAVYFGFIFLGEL
                     DFGYYGTGVTLFIGFFCLMVAPEFYQPMRELGVFYHDKAAAISAADNIETFLKEKVKT
                     QGGNLQENVENQPLVIQAKDCVIFSPQGKALTQALNFELNAHQHIALVGQSGAGKSSL
                     MNMLLGFLPYEGSVTINGTELRDLNLSQWRAKLAWVGQNPQLMHGSLKENILIGNPNA
                     TDSELAKALQLSKADEFVARLGLEHQVQDSNIGISGGQAQRIAIARALLRPYELLLLD
                     EPTASLDMDSEQQVLAALHNLSREQTTLMITHRVEDLTQCDEIWVMKQGQIIQKGRFT
                     ELEHTGFFAELIHNELVHQGEI"
     gene            complement(7602..11699)
                     /locus_tag="PXE44_000363"
     CDS             complement(7602..11699)
                     /locus_tag="PXE44_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HDL5587909.1"
                     /translation="MTDENKQTATNQTDSAQEPQATQPKKSKWRFLKHGFCLTFILLL
                     LAILFLCTGYGQRKVLSWVDKFVEPLQLGQVQGSLQDGLQLSDARFVMDGVNVTVGQA
                     DLHIGFSCLLHYEMCLNNLSLKDTEVLIDTTKLPPSQPKEETEPFTELNLPLGISAQN
                     INLENIKVKVDDMDIHLNHFHTALSGKGRAITVHPTQLEGLNLLLALKLDETDTEQAV
                     DFAQKNAKTEALSATNPQEIADKVIQEAVEKTSEKVVEGFHQLQRKTVAQAEQVDNKV
                     DWAAIKAQLAQPLLDKNIRFSLPLDLNIEQIDIANVAIAQKAKDEKGNLIEPISLLNV
                     ASLQLQAQAKDQHINLKSLDFKSDQGDLTASGSLSLQDNYPLSWTLSGHEAKTAKVKL
                     PFNQIEASLSGELYDKTSLKVKTAGAISASLEGNVALAQAKTPFDLSLKSESMRYPFA
                     PQQNGDVLSLENIAMRLNGNLLDYALEAKVSAKGMGVPASSASLTGKGELTHFNIQDL
                     SLNTLEGTAKIDGLVDWSEGVAWRSNLKLQHINTKSLLADWPAVLSGSLSSEGYAGRG
                     NSASGWKADVSNIDIKGSLLQKNLHLSGNLKADHQQLLDVPGLSLVYGENKIDLKGVL
                     GEKSDFYADIKAPNLQGLVPNLKASVNGNAKLSGKVSEPNIDLDLVASNVGYEQFKLQ
                     NLTAKGKITTEKTIQGDLTLGLRQFAYNEIKVGNATLLLKGNEENHSLKLSAKGNPVG
                     ADLQISGKFDRLKEIWEGQLSQVSIQSTEFGQFQADKAVNVKYDNKAINANISAHCWH
                     NRKINLCFPTAFNAGVEGKVPFEIRNFDLAVLQQFLEKNSQLSGIVNAKGDAAWFKNK
                     QPQVNLDLTSNAIKFIQKMEGGKSFPLTVSPLKIDLKMSDNNLTLKSDLRVENNGRLS
                     TDLVMKDLSKARALSGNIHLDQLSLKLIRPLLERGDSVDGNVNARLTVSGTATSPLLH
                     GNLNVTELKARSVTMPFDITGGNLAMNFHGATSTLSGKLQTKQSELRLEGDADWRDLN
                     AWKTRVHAQANRFQVDIPNIAKVAVSPDIQVTATPTLLTLSGNVDIPWARIEVEQLPE
                     SAVSVSGDEVIMDGSVKNKVPFSQRNIPAQTSGGMAINADIKINIGNDVKLKAYGLNS
                     NVNGLLSVRQGKQGLGLYGQVHLREGRFAAYGQDLLIRKGDISFAGSPSQPTLDIEAI
                     RNPEAMEDPTITAGVRVTGLADNPTVKVFSDPAMSQNEALSYILTGRSLESSGDTSSS
                     NAVAAALLSMSLSKSSKLVGDVGSTFGLKDLSVTTAGIGDNTKVEVSASLAPKFRVKY
                     GVGIFAPLTELTLRYNLTPRLYLQWVSSINQAVDLMYRFEFDELF"
     gene            complement(11780..13615)
                     /locus_tag="PXE44_000364"
     CDS             complement(11780..13615)
                     /locus_tag="PXE44_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HDL5587910.1"
                     /translation="MNTKLHFLTFCCLVAFASMAQANQEQRLSDVDRERVENLQEKLA
                     EDSDDEPEIEPLVTLEVKGIQGKDAKKNVELYLDQIANEYADGSERYQYLVQTTVDKA
                     LRALGYYNSRYQFVQLPQEGKKPLLVLNVELDKETVKIDETDIVLQGDAKTDEDFAHL
                     VSKAPKQGSRLNHKTYDNFKGDIESTAFSKGYFDGHWLYHRLEVYPTDHKADWRLGYD
                     SGKRYHYGQIRFVDSQIKEEYLQNILKIHSGEPYLANDLSILTQDYLSSNWFSSVMVE
                     PHLNEQDKLVDLNVLFQPKKRNEVEIGIGYETEVGPRLQLNWKKPWLNSRGHSIESRT
                     YVSKPEQTFEFGYNIPLREDPLHYYYQLSGTLENEDQNDTKSTAATLGLQRFWTHETG
                     WSFSVGLKARYDSFTQANDRFKTLLVYPTASLNRTRTDGKPYPLWGDTQKLTVNWGSR
                     AVGSDVNFYSYKASTSWARTYFDNHRFYLRAELGYLKANQFERIPPALRYFAGGDMSV
                     RGFGYKDISPRDPSNGKRVGGSHLATGTVEYQYQVYPNWWGAIFYDAGLASNKFKTQD
                     LYYGTGLGVRWASPIGAIKFDVATPVKSPDNSKGIQIYIGLGSDL"
     gene            complement(13627..14472)
                     /locus_tag="PXE44_000365"
     CDS             complement(13627..14472)
                     /locus_tag="PXE44_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDL5587911.1"
                     /translation="MFKQINKRLNFNIFLQMFVLFTPLVQAAQMAIVIDDVGYRIKED
                     REILALPKAVSVAIIPVAPYATERAKDAYNQKRDILIHLPMEPKSKQPIEEGGIHIGD
                     NEEKIRKLIHTSRGQVPYAIGLNNHMGSGATSDSATMQHLLKVLKENTLFFLDSKTIG
                     SSVAAKTARQFGINTLERDIFLDDSDLLADVQKQFAHAINHARKNGVAVVIGHPRKNT
                     ISVLKQNLAQLPQDIELVSVGNLWRNEKTVPDKPFIMLFEQEPALSSVPPYDSIPLLR
                     GIPKE"
     gene            complement(14473..15636)
                     /locus_tag="PXE44_000366"
     CDS             complement(14473..15636)
                     /locus_tag="PXE44_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL5587912.1"
                     /translation="MLVKRINSYCWTAIFSGVISFSTPFSFANNLNQIQQKIQQQTSK
                     INEQKQKRNALQSTLKNQEVEMGKVLDKLQKTEMSLTEVRQTIKATETEIARLEKLEK
                     QQKEKLKEQLDSAYRSGVNPSVLERLMSEEAKNAERMTAYYQHMNNIRIDAIVEIRKT
                     QADLKARRDELKGQQKDHQTQLNEQKKQEKDLKKVQSQRESTLRLIDKTLEQDQSRLE
                     NLRSNEQALKQQLEQAQREAQQAEKREQQREKQAKSGQSKTTENVERTPIKAGRYTMP
                     VSGNIITPFGNNWHGVVIGASAGTSVRAMASGRVIMAQWLAGYGNMVAIDHGNGDISL
                     YGYNQSISVSKGSRVQGGQVIAKVGNSGGQSRSALYFGVTRKGTPINPLNLVK"
     gene            15804..16487
                     /locus_tag="PXE44_000367"
     CDS             15804..16487
                     /locus_tag="PXE44_000367"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL5587913.1"
                     /translation="MQLVFIRHGFSEWNAKNLFTGWRDVNLTERGIEEAKSAGQKLKA
                     AGFEFDIAFTSVLTRAIKTCNIVLEESDQLWIPQVKNWRLNERHYGALQGLDKKETAE
                     KYGDEQVHIWRRSYDISPPDLDPQDPNSAHNDRRYANLPKDLIPNAENLKITLERVLP
                     FWEDHIAPALISGKRVLVTAHGNSLRALAKHIEGISDADIMDLEIPTGQPLVYELDDN
                     LKVISKRYL"
     gene            complement(16624..17181)
                     /gene="frr"
                     /locus_tag="PXE44_000368"
     CDS             complement(16624..17181)
                     /gene="frr"
                     /locus_tag="PXE44_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL5587914.1"
                     /translation="MINEIKKDTQDRMEKSLEALKGHIAKIRTGRAQPSLLDGIQVEY
                     YGSATPLRQVANVVAEDARTLAVNVFDKSLISAVEKAILTSDLGLNPSSAGTTIRVPL
                     PPLTEERRRDLIKIVKAEGEQGKVAIRNVRRDANDQIKALLKDKEISENEQHKAEEDI
                     QKITDTFIKKVDEVLAAKEKELLDF"
     gene            complement(17233..17949)
                     /gene="pyrH"
                     /locus_tag="PXE44_000369"
     CDS             complement(17233..17949)
                     /gene="pyrH"
                     /locus_tag="PXE44_000369"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL5587915.1"
                     /translation="MSKPIYKRILLKLSGEALQGSEGFGIDPSILDRMALEIKELLAE
                     GVEVGVVLGGGNLFRGAKLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAEVNAKLM
                     SAFQLNGICDTYNWSEAIKMLREKRVVIFSAGTGSPFFTTDSAACLRGIEIEADVVLK
                     ATKVDGVYDKDPAKYADAKLYNKLTYAQVIEQELQVMDLAAFTLARDHGMPIRVFNMG
                     KPGALRNVVFGTEEGTTISE"
     gene            18041..19603
                     /locus_tag="PXE44_000370"
     CDS             18041..19603
                     /locus_tag="PXE44_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL5587916.1"
                     /translation="MKSKQLFGILVAISLATAASCLMLIGSGFFPNPSIFEVILGVFL
                     IIALASSKWTYYLLLLPISIAYAIYTPVGIKFGKPTYEYIASIFATDMMESKEFIAQL
                     PILNIAAGIAIVLAVIFYRKITNRYQIRFLRNKTFVISAFVLMLFSLAPFKFFQEFYN
                     SSLKVKKELEVLNNLTLDSQWGQSTLSSTSNYDDYVLIMGESARKDYHHAYGYPAANT
                     PFMSSAKGTLIDGLTSGGTNTIASLRLMLTKPDTEKWEANYGLTLIDLIKSTGIKTYW
                     ISNQGYLGNYDTPISSIGNKSDVKIFTKAGDSLNTNVSDFELLPHFAKVIEEPAQGKR
                     FIFLHLYGSHPITCDRLTDYPKMFDDDKLPKRYFNVNCYVSSIKKTDELLKRVYDLLV
                     KNQENHQRSFSMVYLSDHGLSHEVSKENIVIHNGKDRSKLHYDIPLFKISSDDTQRNE
                     YKAFKSGLNFTNGIANWIGIENPLLDKEINLFNEQNDKSDYGLKKIIETQSTVEDPAV
                     PIPESYLYKATN"
     gene            complement(19667..20518)
                     /gene="tsf"
                     /locus_tag="PXE44_000371"
     CDS             complement(19667..20518)
                     /gene="tsf"
                     /locus_tag="PXE44_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL5587917.1"
                     /translation="MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKS
                     GQAKAAKKAGRVAAEGVILARVASGFGVLVEMNCETDFVAKDAGFLELANEVADFAVA
                     NKGTTIEALQAQFEEKRAALVAKIGENMNIRRVQYLEGEVIAQYLHGAKIGVLVAGAG
                     SEDELKKVAMHVAASKPEFVNPEDVSADVVAKEREIQIEIAMNSGKPKEIAEKMVEGR
                     MAKFTGEVSLTGQPFVMDPSQSVGQYLKSVNTSVSNFIRLEVGEGIEKVEEDFAAEVA
                     KITGGNA"
     gene            complement(20614..21333)
                     /gene="rpsB"
                     /locus_tag="PXE44_000372"
     CDS             complement(20614..21333)
                     /gene="rpsB"
                     /locus_tag="PXE44_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL5587918.1"
                     /translation="MAQVSMRDMLNAGVHYGHQTRYWNPKMKPFIFGARNGVHVINLE
                     KTLPLFNEALAELTRIASNNGKVLFVGTKRAASDAVKAAAVESQQFYVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQTQDGTFDKITKKEALMRTRELEKLELSLGGIKDMNGLPDA
                     IFVIGADHEHIAIKEANNLGIPVFAIVDTNSTPDGVNYIIPGNDDATRAIQLYLDSAV
                     AAIKEGRGVETVEVAEEASAE"
     gene            21678..22706
                     /locus_tag="PXE44_000373"
     CDS             21678..22706
                     /locus_tag="PXE44_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5587919.1"
                     /translation="MKKTLSALAIAAATFTSTSTLAANEVNVYSYRQPYLIEPMLKNF
                     EKDTGIKVNIIFADKGLVDRVKQEGELSPADVLLTVDISRVMEIVNADLAQKIDSKVL
                     EKNIPAQFRDSNDQWFGLTTRARVIYTSKDRVGKLPAGFDYLDLAKPEYKGKVCVRSG
                     KNSYNVSLFAAMIEHYGIEKTKAFLEGLKANLARKPQGGDRDQVKAIKEGICDYSIGN
                     SYYYGKMLDDEKQKSWAEAAIINFPSGEHGTHKNISGVVIAKHSPNKANAVKLIEYLS
                     GEKAQGLYAELNHEYPVKEGIEPSAIVKGWGTFKSDTIKLEDIAKNYEAALKLVDEVK
                     FDDFSEKK"
     gene            22829..24475
                     /locus_tag="PXE44_000374"
     CDS             22829..24475
                     /locus_tag="PXE44_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5587920.1"
                     /translation="MRSQFCWKSIAALTTFIVLLPLIAICYHALQGDNENLAHLWQTM
                     LPIYLKNSTLLVLGTVFLALIFALPTAWIVANYRFWGQRTLQWLLCLPLAMPAYLIAY
                     LYTDLLDYSGAFQGWLRSIFGWTHPQDYWFPQIRTLYGACFVLALVLYPYIFLLVRVA
                     LLEQSENLTHSAKMLGASPSQLFRRITLPLIRPSVAVGIALVAMETLGDFGTVAYFAV
                     PTLTTAIYDSWLGYHDLGTASQIAIFMLLMIFLFLSLEQYSRRKQKSYQRGYEKKSAF
                     KFLTGWKLALALAWCWGLLALAFFIPFGRLLYWAYDYFEQAWNLDFVQFSLNSLKVSI
                     IASVITVLIALLLHFSKRLNTRKILVKNDRLSLQLASLGYAVPGTVLAIGLLSPLTFA
                     DHQLHAWLKSLELSPVGLIFSGSLFALVLAYTIRFLAMAIGSLETSLNKISPSLDMAS
                     QTLGKNGSAMLVKIHIPLIYKGILTAGLMVFIESMKELNASLLLRPFNFDTLATYVFT
                     FTSDEQLERAALPAIVLVLVGLLPVIWLTRSLISQSEKER"
     gene            24485..25495
                     /locus_tag="PXE44_000375"
     CDS             24485..25495
                     /locus_tag="PXE44_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5587921.1"
                     /translation="MSVLTINHLNLNFGSTKVLQNLHLAVNQNEIICLLGASGCGKTT
                     LLKAIAGLLPIEQGDIHLAGECLKSKAVEERQIGLIFQDYALFPHLTVADNIQFGLSK
                     LPKVQQKAITEKMLSVVHLQGFESRFPHELSGGQQQRVAIARALACQPELLLLDEPFS
                     NIDSQTRYAMIQEIKQILKSQNVPAIFVTHSKEEAFAFADKIAVMDQGKIIQFGKPNE
                     LYHSPVNPFVADFLGGTNYLNCNVTEDNVLCSPIGEFRLFPEMNVQKGRYRWLLRPEQ
                     IILKPNELGLGKVVGKLFLGLYYRYQVEIKGIEIVTYQSQELKIGEQVEVGFQVREFI
                     LF"
     gene            25505..26131
                     /locus_tag="PXE44_000376"
     CDS             25505..26131
                     /locus_tag="PXE44_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HDL5587922.1"
                     /translation="MKIEVWSDYACPFCYIGKRHLEQALAQFEGEVEVVFRAFELDPH
                     ANGEPEGDIQQRLMRKYQKTAEQADEMIRYVEQAGKQAGLDLRYRTTQYTRTFEAHRL
                     AKFAESKDLGEAMVERLFKAYFTDNTILAKRTELISLALDIGLERDEIAQLLTGDDFG
                     HEVREDERVAHKYGIHSVPFFVINEKLGVSGAQPPEILLDAIKQALQK"
     gene            complement(26193..27683)
                     /locus_tag="PXE44_000377"
     CDS             complement(26193..27683)
                     /locus_tag="PXE44_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDL5587923.1"
                     /translation="MSLYQIARTETGKVISLNAKMANRHGLIAGATGTGKTVTLRKLA
                     ESLSDDGVPVFLVDVKGDLSGSVQAGNFSGKIAERILQFNLGGEDYLKGYPVSFWDVF
                     GTSGIPLRITISEMGPMLLSRLLNLNDTQGGLLNLVFRVADDRGLLLIDLKDLRALLK
                     FVADNAKEFQLEYGHISAASVGAIQRALLRLENEGADNLFGEPALDLHDWIQTRNGRG
                     VINMLNAEKLINSPRVYSAFLLWFMAELFENLPEVGDPEKPTFVLFFDEAHLLFDDAP
                     KALVDKIEQVVRLIRSKGVGVYFVTQNPLDIPDSVLGQLGNRIQHALRAFTPRDQKAV
                     KSAAETFRANKEVNVVEAITQLGVGQALVSVLDEKGMPTPVEIAYIYPPKSQLAPLAT
                     GERDSLVKQDDLYHHYSQYVDNESAFELLNAKAEAIKAEQAAAEEANNDFFGGILSSI
                     FGSKKKKDQSITEQVVSQVAHSVGRNLRNQVTKQIMRGILGAITKK"
     gene            complement(27742..28977)
                     /gene="rhlB"
                     /locus_tag="PXE44_000378"
     CDS             complement(27742..28977)
                     /gene="rhlB"
                     /locus_tag="PXE44_000378"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDL5587924.1"
                     /translation="MSNHLTEHRFIDFPIHANVLNALDSKGFEFCTPIQAKTFPITLK
                     GKDIAGQAQTGTGKTLAFLIATFNHLLNNEIETSKNQPRALILAPTRELAVQIGADAE
                     LFLKHSDLKLALAYGGDGYDKQVQEIEKGVDILVGTTGRVIDYVKQGIINLDKIQVIV
                     LDEADRMFDLGFIKDIRYLMRKAPKPSERLTLLFSATLSAKVRELAYEDMDNAEYIEI
                     EPLQKTGHRIKEELFYPSNEDKMALLLTLLEEEWPERCMIFANTKHKCEEIWQYLSAD
                     GHRVGMLTGDIPQKKRLSLLDNFTKGNLDILVVTDVAARGLHIPDVTHVFNYDLPDDK
                     EDYVHRIGRTGRAGESGHSISFACERYAVNLPAIEEYIGHQIPVSQYDKESLLTLPKV
                     NRSRSKKTFLAKTKKNSRN"
     gene            complement(29065..29787)
                     /gene="recO"
                     /locus_tag="PXE44_000379"
     CDS             complement(29065..29787)
                     /gene="recO"
                     /locus_tag="PXE44_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL5587925.1"
                     /translation="MTIDQWQRAFVLHRREYSENSLLVEFFTEHHGRITLLAKGARRA
                     RSPLKAVLQPFTPLLLRWSGKGDLKTLTKAEPAALTLPMETLSLYSGFYINEVLARVL
                     ENQTAYPELFQHYLRCITELATQKQIEPILRTFEFHTLKALGYGVDFTHCAATGLPVD
                     PKMTYQFQENQGFIASLLQNNQSFLGSDLLAFAQLDFSEKSTQQAAKRFTRMALKPYL
                     GSQPLKSRELFQSILPNKLKSG"
BASE COUNT         8576 a         6517 c         5555 g         9205 t
ORIGIN      
        1 gtaatgtccc ttttttgaat gtctactaca aaatgggata ttttacaaac cgattgacta
       61 atgaagcaag aatataatta actacaactg gatatatttt gcaaatttcc cgataaattt
      121 caccgcttgt agataaaaac cgtagataaa aaccatagat aaaaaatagg agctaaacat
      181 ttttagcccc taccgtcatc agaaataaat cccgactaaa atcgccacga acacacctaa
      241 gccgaaatag tggttattta gaaacgcttt aaagcattcg ctacgttctc tttttttggt
      301 cagccaacat tggtagataa acagcgtggc acttaaggct aatacgacaa aatagccgat
      361 gtgataatct tttaccacgc ccaataaccc caataaaatc aaggtgataa actgtaataa
      421 cgcaataatt ttattatcgt attgagcgaa taaaattgca gtggatttaa ctccaattcg
      481 taaatcatcg tcacgatcaa ccattgcata ttgggtgtcg taggcgactg tccacgctag
      541 attggcaaaa aacagcagcc agcattcaag tggaagagtt tccgttaccg caccaaatgc
      601 catcggaatc gaccaaccga atgccattcc aagcactact tgtggcaagt gggtaaaacg
      661 cttcataaag ggataaatca ccgccagaaa aactgccaca aaggaaagca gaaacgcata
      721 gcggtttagc attaaatcga gcagaaaagc gaacaacagc aagatggcaa aaatgacttt
      781 tgcctcagtg gtggtcactc tgccggtagc taatgggcgt tgagaggtgc gtttaacgtg
      841 tccgtcaatt tctctatcgg cataatcatt aatcacacaa cctgccgcac gcataacgat
      901 taccccaagg gtaaaaatgg ctaaaatcgc aacaggcggc attccctctg cggcaataaa
      961 taacgcccat agtgtggggt gtaataataa taacgtgccg atgggtttat cgaaacgcat
     1021 taactgggcg taggcaatcc atttttgttt agaaaaaaga gtttcgttat tcattacaaa
     1081 ttttgacgat aacgtccgat tggcttaaat gagtctgtaa ttctaacggt aaatcttgat
     1141 ctgtaaacaa ataatttact tctttaacat ttcctaaacg tgcaatagct gtacgggtaa
     1201 actttgaatg atcggtcact aaaaccactt gtcgggaaca ttccattaat gcacgcttta
     1261 cctgcacttc gtggtaatcg taatcgagca tagagccgtc catatccacc gcactaatgc
     1321 ctaaaatccc aaagtccaaa cggaattggt taataaagcg aaccgtttct tctccgatta
     1381 gcccgccatc acttcttagc cctccgcctg ctaccgtaat ttggcaatct tcattttgca
     1441 tcagaatatg agccgcattt aaattattgg taactacttt taaattttta tgcgaaagta
     1501 aggctaatgc cactgcttct gaagtcgtgc caatatctaa aaataatgat gctccatttg
     1561 gaatcagttt tgccacctgt tgggcaatcg catttttctc gtgtgagaaa aattgcttgc
     1621 ggtgggtgta atcactattc tccgtattgg aaggagcggc tgccccaccg tgatgacggc
     1681 gaatcagatt attttctgcc atttcattca gatcacggcg aatggtttgc ggactcacat
     1741 tcaattcagc cactaattgc tcggtactca catagccaag ctgattaacc agctcaatga
     1801 tctttttgtg tctgtttgcc tgtttcatat ttcctctttt gcaaataatt tgcagaaaaa
     1861 gaccgcttgc aagcggtctt ttttcagtta aattttacaa tttatcaaca agttcaaccg
     1921 catagccgat atagcttgcc ggtgtcatct ctttcaaacg atttttttca ttttccggaa
     1981 tttccaactt atcgataaac tcacgcattg cggtttcatc aacccgtttg cctcgagtca
     2041 gctctttaag tttttcatac ggtttttcaa tgccataacg acgcataaca gtttgaattg
     2101 gctctgccaa tacttcccag ttttggttga gttcatcacg caagtgttgt tcattcactt
     2161 ctaatttgct aatacctttt aaggtggacg cataagcaat taacgcataa cctaaaccta
     2221 cacctaaatt acgcaacacg gtggagtcag ttaagtcacg ctgccagcga gaaatcggca
     2281 atttttgccc caaatgagcc attaccgcat ttgccaagcc aagattgcct tcggaatttt
     2341 caaaatcaat cgggttcact ttatgtggca ttgtgctaga gccgatttca ccggcaatag
     2401 tgcgttgttt gaagtgattt aaggcaatgt agccccacat atcacgatca aaatcaatta
     2461 aaatcgtatt aaaacgagcc acgcaatcaa agaactccgc aatataatcg tgcggttcaa
     2521 tttgggtggt gtatgggttc cacgtcacac ctaacgattc cacaaattct tggctgaaag
     2581 tgtgccaatc aatctccgga tatgctgata aatgtgcgtt atagttgccc actgctccat
     2641 tgattttagc gagaatttct aaattttcta actgtttata ttggcgttgt aaacgatatg
     2701 ccacatttgc catctcttta cccattgtgg tcgggcttgc cggctgaccg tgtgtgcgag
     2761 aaagcaacgg aatatgttgg taacatttcg ccagctctgc tacagcatca atcacttttt
     2821 tccattcagg taataatact tcttcacgag cggttttaag cattaaagcg tgcgaagtat
     2881 tattgatatc ttcagaagta caagcaaaat gaataaattc attcaccgct tgtaattcag
     2941 gcaacgcttc acatttctct tttaggaaat actccaccgc ttttacatcg tggttggtgg
     3001 tacgttcaat cgttttaatc cgattggcat cttctaacga aaaatcagcc acaatttggt
     3061 ttaggtaatc gtttgctttt tcagaaaaca ctggaacttc attaatttgt gtatgcgatg
     3121 ccaatttttg taaccagcga acttccaccg tcacacggaa ttttaataag ccaaattcac
     3181 taaaaatcgg acgaaggctc gcaactttgt cttgataacg tccatcaatc ggggataacg
     3241 cagttaaagc ggtaagttcc attgttttat attctccaaa taggtaaatg ttcggttaga
     3301 tttgacgata aaatttctgt gcttggttaa aaattttctt gcgtgaaaat aaaaattgcc
     3361 aacggcttcc gccgacttgc tgccacaaaa taccggctcg aataccggct agtagcgagg
     3421 ctcgaatttt attctgtata tccgggcgaa ccaagtaatc ttgcattccc atcacgtgaa
     3481 ttttcgtacc taacgggctg ataatatcgc tataaatagc ggctaaattg gcaatcattt
     3541 gatctgccat cacattatct tcatacaggg ctaactggcg ttccacttgc tgtaaacgct
     3601 gggctaactg ttgttttgct tcagggtttt tattgagttt ttggctaagg gcgagcaggc
     3661 ttatccaata acgtccgact tcggtatcta atttgccgtt accgccgcca gtttgagcta
     3721 atgcggtttc taagcccatt tttaaatggg aaagatcatc gccaaataca cttaacgttg
     3781 attccggctg agtgattaac aggctttcta aagcacactt aaaagcctct ctgtctgcca
     3841 tgcctttatg tgcaaattgt tgcactaacg tcgccgcttg gcaaacgccg gcataagcaa
     3901 gggtaatatc gtggtaatcc gccatctctg taaaattcaa tatgataaaa cattagtggg
     3961 aatatagcat tttttcagct tcttttgaat ggcaaaaggc atttattcta ttgaaaaata
     4021 aatgcctttt cattaatacg caatttgctg caaaaactat cctaccaatc gcccattatc
     4081 tattcgataa accacatcaa atttatccag tccgctttgg cggtgagtga tcataattaa
     4141 ggtgcgatca cagcagtgag tcaaaattag gtttaaaatc tgctgttcgg tttctctgtc
     4201 cagtccttct gttggctcat caagtaagac taattcagca gaactcagga gcaaacgggc
     4261 aagccctaaa cggcgttgtt cgccaccaga aagcggtctg ccgccctcgc ctaaccataa
     4321 atttaagccg tctgtttcaa gcaaatgccc taagcctact tttcttactg cttcttgcat
     4381 ttgttcgtct gtagcttgag cattccccat taataaatta tcttttaggg tttggctaaa
     4441 aatatgcact cgttggctta aggtaactaa ttttgagcgt aacatcggct ctgaatattc
     4501 gtcaatttta caattattca gccaaatttg accgcttgta gggtcgtagt tgcggtttaa
     4561 cagttggaat atggtcgatt ttccgctacc cgttttacct aagattgcca ctttctgccc
     4621 ttttaatacc ttaaaggaaa gattatttag tacgttttgc tcgccgtaat aagcaaagct
     4681 cacgttttca cagcgtacaa gcggttgatt ttggctcaaa ttttgccatt ctgccttgct
     4741 accaaatgtt acattaggct gttgttcggt aatttcacta atccgctcgg cggcggtaat
     4801 cacttgccct aaatgcaaaa aggcgatacc aatcggggaa agaatctccg ccgatgccat
     4861 tacacagaaa atcactaacg caattaaggc ttcagggtat tttgcagtgg gcacattaat
     4921 cgaagttgaa accaaataga tcaccacaca agtcaaaatg ccgttggata acattatcaa
     4981 gctatttgat aagccggaca gtcggctttc tttgctttga gcgttcagcc attgttgttc
     5041 agtttgctgt agtttttcgc tcatttggtt caagttgcca aagagtaaaa attctgcatt
     5101 taactgtacc cactcaataa attggctgcg gtaatttgct cggctttgta ttgcattacg
     5161 ccctaatttc aaacccaatc gataaaatac ggtaggaata acggcaagca ataccaatag
     5221 tgtgccgcaa atgatcagca ttaatggcac ggaaacaaaa gaaagcccga ttgccataaa
     5281 tgcaataatc atcactgcac tgacaaaagg cgagactaaa ttcagatata gggtgtctaa
     5341 cgtatccaca tctgccacca agcggtttaa caattcgctg tttctaaagc gatttagccc
     5401 gcttggactc agtggaatca attttttaaa gacgctagca cgcaaattcg ccaatacccg
     5461 aaaagtcgca tcgtgggtga ctaaacgttc aaagtaacgt gaaattgtcc gccctactgc
     5521 caaccctctc acccccgatg aagggtagaa aaagttaaaa ataagcgcat tgccggctag
     5581 aaatgaggca gctaaaaacc aaccggaaag gctgagcaaa ccgatacttg ccgctaatcc
     5641 taaaattgcc agcacaacac ctaataatag tcgcccaaaa tgggtttggt agagagcaaa
     5701 aaacgggaac aacgatttca ttagatttct ccctgatgca ctaattcatt atgtattaat
     5761 tccgcaaaaa agccggtgtg ttctaattca gtgaaacgac ctttctgaat gatttgccct
     5821 tgtttcatca cccaaatttc atcgcattgg gttaaatctt ccactcgatg agtaatcatt
     5881 agcgttgttt gctctcggct gagattatgc agagcggcta acacttgctg ttccgaatcc
     5941 atatcaaggc ttgcagtcgg ctcgtctaac agtaataatt cgtacgggcg aagcaacgct
     6001 cgtgcaatcg caatccgctg tgcttgcccg ccagagatac cgatattact gtcttgcact
     6061 tgatgctcca accctaaacg tgccacaaat tcatcggctt ttgaaagctg taatgccttg
     6121 gctaattcag aatctgtcgc attagggttg cctattaaaa tattctcttt caaactgcca
     6181 tgcattaatt gcggattttg ccccacccac gctaatttcg ctcgccattg gcttaaattt
     6241 aaatctcgca gttcggtgcc gttaatcgta accgaccctt cataaggcaa gaaaccaagt
     6301 aacatattca ttaatgaact ttttcctgca ccactttgcc caaccaaagc gatatgttgg
     6361 tgtgcattca actcgaaatt taacgcttgg gttaaggctt tgccttgtgg tgagaagatc
     6421 acgcaatctt tggcttgaat cacaagcggt tgattttcaa cattttcttg caaattgcca
     6481 ccttgcgttt tcaccttttc ttttaagaaa gtttcgatat tatctgcggc actaatcgct
     6541 gctgccttgt cgtgataaaa cacgccaagt tcacgcatcg gttggtaaaa ctcaggtgcc
     6601 accattagac aaaagaagcc gataaataag gtcacgcccg taccataata gccaaaatcc
     6661 agctcaccta aaaaaataaa gccaaaatag accgccgtca ccgcaatcga taccgccgtg
     6721 aaaaactcca ataccgcaga ggataaaaac gccattttta gtacgtccat cgtactgatt
     6781 cggaaatctt cggtgctttg gtagatttgt tcagtctgct tttccgcttg cccaaataag
     6841 cgaatggttt ctaaaccttt taatttatct aagaactgtc cgctaatacg ggaaagaatg
     6901 ccgatattac gctgatttgc ctccactgct ttcattccgg ctaaaatcat aaataaaggt
     6961 aataatggca ttgttgcaaa taaaatagcc cctgctgccc agttaatcgg gaatacaaaa
     7021 cagaggatca gtaatggcac tatcagcgat aaaaattgct gtggcaaata gcgtgcgtag
     7081 aaattatgta gattttccac ctgctcgagc attaaggttg cccagctgcc cgccggtttt
     7141 tgttgaatgc tcatcggccc gactgcttcc attttggcta gaatttggcg gcgaagatgt
     7201 aaacgcaagg tttgccctgc tttgaaaccg acacgctcac gcagaaaaat gaagaatgct
     7261 ctggcagcaa aacaacagaa taagagcata atttcaacca gaaagctatt cggtgactgc
     7321 tttccgataa gcattttgtc taacattgtg gcaatcagcc acatctgtcc aatcatacat
     7381 aaactactga aactccctaa cagtatgttt aagtgcatat attttttgat gactttttgc
     7441 tgacttttaa gccatttttg taattcgcgt tggcgttgtt tttccataat tttgtcaatt
     7501 tttttgtttt ttcaaccgct tgtaaatcgg gtttgccatt ctactataaa gcaaaaaggt
     7561 tgttaatggc gaaacattaa caacctttca ttcagcctta actagaatag ctcgtcaaat
     7621 tcaaagcgat acattaaatc taccgcttgg ttaatacttg agacccattg taagtacaat
     7681 cttggcgtta aattatagcg taaggttaat tcggtaagcg gtgcaaaaat gcccacgcca
     7741 tacttcactc tgaatttagg ggcaaggctg gcacttactt ctactttggt gttatcgcca
     7801 atacctgcgg tggtcacgct taaatctttg agtccgaaag tgctaccaac atcacccacc
     7861 aatttactgc tttttgataa gctcatactg agtaatgccg ctgcgactgc attactggag
     7921 ctggtatcgc cactgctttc caatgaacga ccagttaaaa tataggaaag tgcttcattt
     7981 tgcgacattg ccggatcaga gaatactttt accgttggat tgtctgccaa accggtcact
     8041 cttaccccag ctgtaatggt cggatcttcc attgcctccg gattgcgtat agcttcaata
     8101 tctaaggttg gctgagacgg tgagccggca aaactaatat cacctttacg aatcagcaag
     8161 tcttgaccat aagcggcaaa tcgtccttca cgcaaatgaa cttgcccgta aagccctaaa
     8221 ccttgtttgc cttgcctgac agacaataag ccattcacgt tactgtttaa accataggct
     8281 ttgagtttga catcattacc gatgttaatc ttaatatctg cattaattgc cattccacct
     8341 gaggtttgag ccggaatatt acgttggcta aacggcactt tatttttcac agagccgtcc
     8401 ataatcacct catctccact tacactgacc gcactttcag gtaactgctc gacttcaatt
     8461 cttgcccaag ggatatctac atttccactt aaggttaaaa gagttggcgt tgccgtcact
     8521 tgaatatccg ggctaactgc cacttttgcg atattaggaa tatccacttg gaaacggtta
     8581 gcttgagcgt gaacccgtgt tttccacgca tttagatctc gccagtctgc atcaccctcc
     8641 aaacgcagtt cgctttgttt ggtttgtaat ttaccgctta aagtcgatgt tgccccgtgg
     8701 aaattcattg ctaaattccc acctgtaata tcaaacggca ttgtaacaga gcgagctttt
     8761 aactctgtaa cgtttagatt tccgtgcaat aacggagagg ttgcggtgcc gctcacggta
     8821 agacgagcat tcacattgcc atcaaccgaa tcgccccgtt caagtagcgg tcgaatcagc
     8881 tttaagctta attggtcaag atgaatattg ccggataacg cacgagcctt acttaaatct
     8941 ttcatcacta aatcagtcga aagtctgccg ttattttcga cccttaaatc agatttaagg
     9001 gttaaattat tgtcagacat tttcagatca attttaagcg gtgaaacggt gagtgggaaa
     9061 ctttttcccc cctccatttt ttgaataaat ttaatcgcat tagaggttaa atccagattc
     9121 acttgaggct gtttgttttt aaaccaagct gcatcgcctt tggcattcac aatccctgaa
     9181 agctggctgt ttttctccaa aaattgttgc aataccgcta aatcaaagtt acggatttca
     9241 aaaggcactt taccctcaac accggcatta aatgcagtcg ggaaacaaag attaattttc
     9301 cggttatgcc aacaatgtgc ggaaatattg gcgttaatgg ctttattgtc atatttcaca
     9361 ttcactgctt tatctgcttg gaattgacca aactcggtgg attgaatcga aacttggctt
     9421 aattgccctt cccaaatttc tttcaaacgg tcaaattttc cagaaatttg caaatcagca
     9481 ccaaccggat tgccttttgc acttaatttc aagctatgat tttcttcatt tccttttagc
     9541 aataaagtgg catttcccac tttgatttca ttataggcaa attgacgtaa ccccaaggtt
     9601 aaatcccctt gaatggtttt ctcagtggta attttgcctt ttgcggttaa attttgcagt
     9661 ttaaattgct catagcctac atttgaggcc actaaatcta aatcaatgtt aggttctgaa
     9721 accttacccg ataatttggc attaccattc acgctggctt ttaaattcgg cactaaccct
     9781 tgcaaattag gggctttaat atccgcatag aaatcagatt tttcgcctaa cacgcctttt
     9841 aaatcgatct tattttcacc atacaccagc gataagcccg gaacatctaa aagttgttga
     9901 tgatcggctt ttaaattacc gcttaagtgt agatttttct gtaataacga acctttaata
     9961 tcaatattcg agacgtccgc tttccatccg ctagctgaat tgccccgtcc tgcataacct
    10021 tcggaagata aagaaccgga caataccgcc ggccagtctg ccagtaggga tttagtgtta
    10081 atatgctgaa gtttcaaatt tgaacgccac gcaacgccct cgctccaatc cactaaaccg
    10141 tcgatcttcg ccgtaccttc cagcgtattg agacttaaat cttgtatatt aaaatgggtt
    10201 aattcccctt tgccagttaa actagccgag cttgcaggca cgcccattcc tttggcactg
    10261 actttagcct ccaaagcgta atccaggaga ttgccattaa gcctcatggc gatattctct
    10321 aaactcagca catcaccatt ttgctgagga gcaaacggat aacgcattga ctcactcttc
    10381 agcgataaat caaagggagt tttcgcctga gccagtgcaa cattgccctc caacgaggcg
    10441 ctaattgcac cagccgtctt cacttttagg ctggttttgt catatagctc accggaaaga
    10501 gaggcttcaa tttgattgaa cggcaatttt actttcgccg tttttgcctc gtgaccactt
    10561 agcgtccaac ttagcggata attatcttgt aacgataaag aaccgcttgc ggttagatcc
    10621 ccttggtcgc ttttgaaatc taaacttttt aggttgatat gctgatcttt tgcttgagcc
    10681 tgaagctgta aagaggcaac atttaacaat gagatcggct caatcaaatt ccctttttca
    10741 tctttagctt tttgagcaat cgctacattc gcaatatcaa tctgttcaat attgagatca
    10801 agaggcaaac taaagcgaat atttttatcc aataacggct gtgctaattg tgctttaatc
    10861 gctgcccaat ccactttatt atccacctgt tcggcctgtg caacagtttt tctctgtaat
    10921 tggtgaaagc cctctacgac tttttcactg gttttttcca ccgcctcttg aatcacttta
    10981 tcagcaatct cttgtggatt tgtcgctgat aacgcctctg tttttgcatt tttttgtgca
    11041 aaatcgaccg cttgttcagt atcagtttcg tctaacttta aggctaaaag cagatttagc
    11101 ccctctaact gtgtcgggtg aacggtaata gcccgccctt taccagaaag tgcagtgtgg
    11161 aaatgattaa gatgaatatc catatcatca actttgacct tgatattctc taaattgata
    11221 ttttgagctg aaatccccaa tggcaaattt aattcggtga aaggttcggt ctcttctttt
    11281 ggctgagaag ggggtaattt agtcgtatca atcaacacct ctgtatcttt aaggctgaga
    11341 ttattcaaac acatttcata gtggagcaag cagctaaaac caatatgcag atctgcttgc
    11401 ccaaccgtta cattcacacc gtccatcaca aatcgggcat cagaaagctg taacccatct
    11461 tgcaggctac cttgcacttg cccaagttgt aatggctcta cgaatttatc tacccagctt
    11521 aacactttac gttgcccgta acctgtgcat agaaagagaa tggcgagtaa aagtagaata
    11581 aacgtcagac aaaagccgtg cttcaaaaac cgccactttg attttttagg ctgtgttgct
    11641 tgtggctctt gtgctgagtc cgtttgattt gtggctgttt gtttattttc gtcagtcata
    11701 atcgtgcata tttatgaggt gataaaaagt aaacaagcgg tatgcgaaat tttactgaaa
    11761 ttcgaccgct tgttttcatt tacagatcgg aacctaaacc aatataaatt tgtattcctt
    11821 tggaattatc tggtgatttt accggcgtgg cgacatcaaa tttaatcgcc ccgataggtg
    11881 atgcccaacg cacccctaag cccgtaccgt agtagagatc ctgagttttg aatttattcg
    11941 atgccaagcc tgcatcataa aaaattgcac cccaccaatt agggtaaacc tgatactgat
    12001 attctactgt gcctgtggct aaatgagagc ctccgacacg tttgccatta ctcggatcac
    12061 ggggggaaat atccttatag ccaaagcccc gcacactcat atcgccaccg gcaaaatagc
    12121 gtaacgccgg tggaatacgt tcaaattgat tggctttcaa ataccccaac tccgccctta
    12181 aatagaaccg atggttatca aagtaagttc tcgcccaaga tgtcgaggct ttataactat
    12241 aaaagtttac atctgaacct actgcacgac tcccccagtt taccgttaat ttttgtgtat
    12301 ccccccataa tgggtaaggc ttgccatctg ttctggttcg attcaatgat gccgttgggt
    12361 aaactaataa ggttttaaat cgatcattag cttgagtaaa agaatcgtaa cgggctttta
    12421 aaccaacaga aaatgaccaa ccggtttcgt gagtccagaa acgttgtaag ccaagggtgg
    12481 cagcggttga ttttgtatcg ttttgatctt cgttttctaa agtgccggat aattggtaat
    12541 aataatgtag cggatcttct ctaagtggaa tattataacc aaattcaaat gtctgctccg
    12601 gcttagaaac gtacgttcgg ctttcaatac tatgacctcg gctatttaac caaggtttct
    12661 tccagttaag ctgtaacctt gggccaactt cagtttcgta gcctatccca atttccactt
    12721 cattgcgttt tttaggctga aataacacat ttaaatccac taatttatcc tgttcattta
    12781 aatgtggctc aaccatcact gatgaaaacc aattactgga aaggtaatct tgggttaaaa
    12841 tcgaaagatc gttagctaaa tatggctcac cggaatgaat tttaagaata ttttgcaaat
    12901 attcttcttt gatttggcta tcaacaaacc ggatttgtcc ataatggtat cgttttccgc
    12961 tatcatagcc taaacgccaa tccgctttat gatcggtcgg ataaacttcc aaacggtgat
    13021 aaagccaatg accgtcaaaa taccctttac taaaagcggt actttcaata tcacctttaa
    13081 aattatcata ggttttatgg tttaaacgac taccttgttt tggggcttta ctgactagat
    13141 gagcgaaatc ttcgtccgtt tttgcatcgc cttgcaaaac aatatcggtt tcatcaattt
    13201 ttaccgtctc tttatctagc tcaacattta atactaagag cggtttcttt ccctcttgag
    13261 gtagctgcac aaattggtat cggctattat aatagcccaa ggctctcaga gctttatcta
    13321 ctgtggtttg caccaaatat tgataacgct ctgaaccatc tgcatattca ttggcaattt
    13381 gatctaaata tagctccaca tttttcttgg catctttacc ctgaatgcct ttgacttcta
    13441 aagtgactaa tggctctatt tctggctcat catcactatc ttccgctaat ttttcttgca
    13501 gattttcaac tcgctcacga tctacgtcag acagcctttg ctcttgattt gcttgtgcca
    13561 ttgaagcaaa agccactaaa cagcagaatg ttaaaaaatg tagttttgta ttcattagta
    13621 acctttttat tcttttggaa tgccacgcaa aagcggaata ctgtcataag gcggaacaga
    13681 acttaacgct ggctcttgct caaagagcat aatgaatggt ttgtcaggca ctgttttttc
    13741 attacgccac aagtttccaa cactcaccaa ttcaatatct tgaggtagtt gtgctaaatt
    13801 ttgcttcagt acactaatcg tattcttgcg agggtgtcct atcaccaccg ctaccccgtt
    13861 ttttcgtgcg tgattaattg catgtgcaaa ctgtttttgt acatcagcaa gcagatcgct
    13921 atcatctaaa aaaatgtctc tctccagcgt attaatacca aattgacgag ccgttttagc
    13981 cgccacacta ctgccaatag ttttactgtc taaaaagaaa agcgtatttt cttttaacac
    14041 tttcaacaaa tgttgcattg tagcagagtc tgaagttgct ccactgccca tatggttatt
    14101 taaaccaata gcatatggta cttgaccacg agaagtatga ataagtttcc gaattttctc
    14161 ttcgttatca ccaatatgaa tacccccctc ttcaattggc tgtttcgatt taggttccat
    14221 tggcaaatga atcaaaatat cccgtttttg gttataagcg tcctttgccc gttctgttgc
    14281 atagggtgca acaggaataa tcgccacgct taccgccttg ggtaaggcta agatttctcg
    14341 atcttctttt attcgatagc ccacatcatc aattacaatc gccatttgag ctgcttgtac
    14401 aagcggtgta aaaagcacaa acatttgcaa aaaaatgtta aaattcaacc gcttgttaat
    14461 ttgtttaaac atctatttca ccaaatttag aggattaatc ggcgtgcctt tacgggtaac
    14521 accaaaatag agtgcagaac ggctttgtcc cccactgtta ccaaccttcg caataacctg
    14581 tccgccttgc actcggctac ccttgctgac tgaaatagat tggttatagc cataaaggga
    14641 aatatcacca ttgccgtggt caatcgcaac catattgcca taccctgcaa gccattgagc
    14701 cattatgact cgcccgctcg ccattgctct aaccgaagta ccggcagaag caccaatcac
    14761 tacaccgtgc caattattac caaaaggagt gataatattg ccagacaccg gcatcgtata
    14821 tcgaccagcc ttgatcggtg ttctctcaac attctcagtt gttttggact gaccggattt
    14881 tgcctgtttt tcccgctgtt gttcacgttt ttctgcttgc tgagcctctc tttgcgcttg
    14941 ttctaattgt tgttttagtg cctgttcatt gctgcgtaaa ttctctaatc ggctttgatc
    15001 ctgctcaagc gttttatcga ttaaacgtaa ggtactttca cgctggcttt gtactttctt
    15061 taaatctttc tcttgtttct tttgttcatt aagttgcgtt tgatgatctt tttgctggcc
    15121 tttcaattca tcacgtctgg ctttaagatc agcttgggtt ttacgaattt caacgatcgc
    15181 atcaatgcga atattattca tatgctgata ataagcggtc attctttccg cattttttgc
    15241 ctcttcagac attaaacgct ctaagaccga agggttcacc ccagaacggt aggcagagtc
    15301 caactgctct tttaattttt ctttctgctg tttttccaac ttttctaaac gggcaatttc
    15361 agtctcagtc gctttaatgg tctggcgaac ttccgttaaa gacatttcgg ttttttgtaa
    15421 tttatccaac accttcccca tttctacttc ttggtttttt agggtagatt gcaacgcatt
    15481 acgtttttgc ttttgttcat taatttttga ggtttgctgt tggatttttt gctgaatctg
    15541 attaagatta tttgcaaaag aaaaaggggt agaaaagctt attaccccac tgaaaatagc
    15601 agtccaacag taagaattta ttcgtttcac cagcatacct ttgtttcttc aaaatattat
    15661 taaggttata ttctaatcgg ttatttgctt aagatcagct ttttagtgaa agaatttaac
    15721 tatctatttc aaaatataaa gaattttgct ataaatagca caatttcaaa tccattaatt
    15781 ttactcaaag ctaaggagtc attatgcaat tagtatttat ccgtcacggt ttcagtgaat
    15841 ggaatgctaa aaacttattt accggttggc gtgatgttaa tttaacagaa cgtgggattg
    15901 aagaagcaaa atctgccggt caaaaattaa aagcagccgg ttttgaattt gatattgcgt
    15961 ttacttcggt tttaacccgt gcgatcaaaa cttgtaacat cgtattagaa gaatcagacc
    16021 aattatggat ccctcaagtg aaaaactggc gtttaaacga gcgtcactac ggtgcattac
    16081 aaggtttaga caaaaaagaa actgctgaaa aatatggtga tgaacaggtt cacatctggc
    16141 gtcgttctta tgacatctct ccaccggatt tagacccaca ggatccaaac tcagcacata
    16201 acgaccgccg ttatgctaac ttaccaaaag atttaattcc aaatgcagaa aacctcaaaa
    16261 tcacccttga gcgtgtgtta ccattctggg aagatcatat tgcacctgca ttaatttcag
    16321 gtaaacgcgt attagttact gctcacggta actcattacg tgcattagca aaacacatcg
    16381 aaggcatttc tgatgcagac attatggact tagaaatccc aaccggtcag ccattagtgt
    16441 atgaactaga tgacaactta aaagtaatca gcaaacgtta cttataattg gtaaatttcc
    16501 gataaaaaat aaccgcttgt agtgcaacac attacaagcg gttatttttt gttaattttt
    16561 tgcaaatggc aaagaacaaa aataaaggcg aatcatacat tcgccttatt gttttaaagt
    16621 agattagaaa tctaacaact ctttctcttt tgctgctaat acttcatcaa cttttttaat
    16681 gaaggtatcg gtgatttttt gaatatcttc ttcagctttg tgttgttcgt tttcgctgat
    16741 ttctttatct tttaataacg ctttgatttg gtcattagca tcacgacgaa cattacgaat
    16801 tgccactttg ccttgctcac cttctgcttt aacgattttg attaaatcac gacgacgttc
    16861 ttccgttaat ggtggaagcg gaacacgaat ggttgtgccg gcagaagatg ggtttaaacc
    16921 taaatctgag gttaaaattg ctttttctac cgcagagatt aaagatttat caaatacgtt
    16981 taccgctaaa gtacgggcat cttcagctac cacgttcgct acttgacgta atggagtcgc
    17041 agagccgtaa tattccactt gaataccgtc taataaagaa ggttgggcac gacctgtacg
    17101 aattttcgca atatgccctt ttaaagcctc aaggcttttt tccatacgat cttgggtatc
    17161 tttcttgatt tcgttaatca ttgatttttt atccttttat ttagtaactg agagttaaac
    17221 agagaaaatt aattattcag aaatggttgt accttcttca gtaccgaata ccacattgcg
    17281 taacgccccc ggtttgccca tattaaagac acgaattggc attccgtggt cacgagcaag
    17341 cgtgaatgct gctaagtcca ttacttgtaa ttcttgctca ataacttgcg catacgttaa
    17401 tttattatat agttttgcat ctgcgtattt tgctgggtct ttatcataaa cgccatcaac
    17461 tttagttgct tttaatacca catcagcttc aatttcaata ccacgcaagc aagcagctga
    17521 atcggtggta aagaacgggc tacctgtacc ggcagagaaa atcaccacac gtttttcacg
    17581 caacatttta attgcttcag accagttgta agtgtcgcaa atcccattta attggaacgc
    17641 agacattaat ttagcattta cctctgcacg gtgtaaggca tcacgcatag ccaaaccgtt
    17701 cattacggtg gcaagcatac ccatatggtc gcccactaca cggttcattc ctgctttggc
    17761 taattttgca ccacggaata agttaccgcc gccaagtacc acaccaacct caacgccttc
    17821 agcaagtaat tctttaatct ctaacgccat acgatccaaa atagacgggt cgataccaaa
    17881 accttcactg ccttgtaggg cttcaccact taatttgaga agaattcgct tataaatagg
    17941 tttgctcatt ttaattgcct cttaactgta tggactaaac acaaaattac gttattatag
    18001 aacaatccaa tataacgata aactaaaaat attcactaca atgaaatcca aacaactttt
    18061 cggcattctt gtcgctattt cgctggcaac tgctgctagc tgtttaatgc taatcggatc
    18121 aggctttttt ccaaatccga gtatctttga agtcattttg ggcgtattcc tcatcatcgc
    18181 tcttgccagc tctaaatgga cttattattt actcttactg ccaatcagca tcgcttatgc
    18241 aatttatacc ccggttggga taaaattcgg taaaccgact tacgagtata tcgcctctat
    18301 ttttgctacc gatatgatgg aaagcaaaga gtttattgct caactgccga ttttaaatat
    18361 tgcggcgggc attgcgattg tactagcggt cattttttat cgaaaaatta ccaatcgcta
    18421 tcaaatccgt tttttacgca ataaaacatt cgtgatttcg gcttttgtat taatgctgtt
    18481 ttcccttgca ccgtttaaat tttttcagga gttctacaat tcgagcctaa aggtaaagaa
    18541 agaattggaa gtgctgaaca atttaacgct agattcacaa tggggacaat caaccctcag
    18601 ctcaacttca aactacgatg attatgtact gattatgggc gaaagtgcga gaaaggatta
    18661 tcaccacgcc tatggctatc cggctgccaa tacgcctttt atgagttcgg caaaaggaac
    18721 cttgattgac ggtttaacct ccggtggcac aaacactatc gcctcattac gcttaatgct
    18781 cactaagccc gatacggaaa aatgggaagc aaattatggg ttaaccttaa ttgatttaat
    18841 caaatctacc ggtatcaaga cctattggat ctcaaatcaa ggctatttgg gcaattacga
    18901 tacgccgatt tcctctatcg gtaataaaag tgatgtcaaa attttcacta aagccggcga
    18961 ttcattaaat accaatgtga gcgattttga attgttaccg cattttgcta aagtgattga
    19021 agaacctgct caagggaagc gtttcatctt tctgcattta tatggctcgc atcctattac
    19081 ctgcgatcgt ctcaccgatt acccgaaaat gtttgacgat gacaaactgc caaaacgcta
    19141 ctttaatgta aattgctacg tgtcatctat caagaaaacc gatgaattgc tgaaacgggt
    19201 atatgatttg ctagtgaaaa accaagaaaa tcaccagcgt tcattctcaa tggtttattt
    19261 gtctgatcac ggtttatcgc acgaggtttc aaaagagaac attgttatcc acaatggaaa
    19321 ggatagaagt aaattacact acgatattcc gctgtttaaa atttcaagcg atgataccca
    19381 acgtaatgag tataaagcct ttaaatccgg cttaaacttc actaatggta ttgcgaattg
    19441 gattggtatc gaaaatccgt tattagataa agaaattaat ctttttaacg aacaaaacga
    19501 taaaagtgat tatgggctaa agaaaatcat tgaaacgcag tctactgtag aagatccagc
    19561 tgttcctatt cctgaaagct acttatataa agcgactaat tagcaaaagt aagtaacaaa
    19621 aaagcaggct ttaagcctgc ttttttagtt tttagtatca gaaaaattaa gcgttaccgc
    19681 cagtgatttt cgctacttct gctgcgaaat cttcttctac tttctcaata ccttcaccta
    19741 cttctaaacg aataaagtta gaaactgaag tatttactga ttttaaatat tgacctacag
    19801 attgagatgg atccattaca aacggctgac cggttaatga aacttcaccg gtgaatttag
    19861 ccatacggcc ttcaaccatt ttttctgcga tttcttttgg tttaccagaa ttcatcgcaa
    19921 tttcaatttg aatttcacgt tcttttgcta ctacatcagc agaaacatct tctgggttta
    19981 caaattcagg tttgcttgca gcaacgtgca ttgctacttt cttaagctca tcttcagagc
    20041 ctgcacctgc tactaataca ccaattttcg caccgtgtaa gtattgagca ataacttcgc
    20101 cttctaagta ttgaacacgg cgaatgttca tattctcacc gattttcgca actaatgccg
    20161 cacgtttttc ttcaaattgt gcttgtaatg cttcgatagt tgtaccttta tttgcaactg
    20221 caaaatcagc aacttcgttc gctaattcta agaaaccggc atcttttgca acgaagtcag
    20281 tttcacagtt catttcaact aatacgccaa aacctgaagc gacacgagca aggattacac
    20341 cttcagctgc aacacgacct gcttttttag ccgctttcgc ttgaccggat ttacgcatat
    20401 tatcgattgc taattcaata tcaccgttcg cttcaactaa tgctttttta cattccatca
    20461 tacctgcgcc ggtacgttca cgaagttctt taactaatga tgctgtgatt tcagccattt
    20521 taaattcccc ttatttgata tggtaaaaca ggctctcacc tgcctagaaa tttcattgtt
    20581 tagggcaggt taaaacctgc cccctcaact gaattattcc gcagaagctt cttctgctac
    20641 ttcaacagtt tcaacaccgc gaccttcttt gattgcagca actgctgaat ctaagtaaag
    20701 ttggatagca cgtgttgcat catcgttacc gggaatgatg tagttaacgc catctggagt
    20761 tgagttagta tcaacaatcg caaataccgg aatacctaag ttgtttgctt ctttaattgc
    20821 gatatgttcg tgatcagcac cgataacaaa aattgcatcc ggtaaaccgt tcatatcttt
    20881 gataccgcct aagcttaact ctaatttttc taactcacga gtacgcatta acgcttcttt
    20941 tttagtgatt ttgtcaaaag taccgtcttg agtttgagtt tctaaatctt ttaagcgttt
    21001 gattgattga cgaactgttt tccagttagt caacatacca cctaaccaac ggtggtttac
    21061 gtagaattgt tggctctcta ctgccgctgc tttaacagca tcacttgctg cacgttttgt
    21121 accaacaaat aatactttac cattgttact agcaatgcga gttaattccg ctaatgcttc
    21181 gttgaataaa ggtaaagttt tttctaagtt gattacgtga acaccattac gagcaccaaa
    21241 gatgaatggt ttcatttttg ggttccagta acgtgtttgg tgaccatagt gaacgcctgc
    21301 gttaagcata tcgcgcatag aaacttgtgc cataagaatt ttccttcaaa gttgtttgtt
    21361 ttgccactta tgaacggcaa aacggggttt agcctccaca tatcctagaa aatcaatctg
    21421 ttaaagacac cccaattcct agcttgtgat atgtgtgtgt tttagtgtta aaaaataaat
    21481 gtgtacgttt tgcctgaata gacaaaaacg gggcgtatta tgccaaaaaa caaacaattt
    21541 gccaattatt ttattgctct cttcgcatta ctaaatcaag ctcaaaaata aaaaccgttc
    21601 tcattattga ctaaaaaaat ggggaggttt agaatgtctt tcattctgtt atgaaaacct
    21661 gtctgaagga gactatcatg aaaaaaacac tttctgcact tgctattgct gcagccactt
    21721 ttacatcaac ttcaacgctt gctgcaaatg aagtgaatgt ttattcttac cgccagcctt
    21781 acttaattga gccaatgcta aaaaactttg aaaaagatac tggcattaaa gtaaacatca
    21841 tcttcgctga taaagggttg gttgatcgtg taaaacaaga aggtgaatta agtcctgctg
    21901 atgtgctttt aacagtagat atttcacgcg taatggaaat tgttaacgct gatcttgcac
    21961 aaaaaattga ctctaaagtg ttagaaaaaa atattcctgc acaattccgt gactctaacg
    22021 accaatggtt tggtttaacc acacgtgctc gtgttatcta cacgtcaaaa gatcgtgtag
    22081 gtaaattacc agcaggcttt gactacctag atttagctaa accagagtat aaaggtaaag
    22141 tctgcgtgcg ttcaggcaaa aactcttaca atgtctcact attcgcggcg atgatcgaac
    22201 attacggcat tgaaaaaacc aaagcattct tagaaggctt aaaagcgaac ttagcacgaa
    22261 aaccacaagg tggtgaccgt gatcaagtaa aagcaattaa agaaggcatc tgtgattact
    22321 ctatcggtaa cagctactac tacggcaaaa tgttagatga tgagaaacaa aaatcgtggg
    22381 ctgaagcagc gattattaat ttcccgagtg gtgaacacgg cacgcacaaa aacatcagcg
    22441 gcgtagttat tgcaaaacac tcaccaaata aagcaaacgc ggtgaaatta attgaatacc
    22501 taagtggcga aaaagcacaa ggtttatatg ctgaacttaa ccacgaatat ccagtaaaag
    22561 aaggcatcga gccatcagca atcgtaaaag gttggggtac gttcaaatca gataccatca
    22621 aacttgaaga tatcgcaaaa aattacgaag ccgcattaaa attagttgat gaagtgaaat
    22681 ttgacgattt ctctgagaaa aaataatcaa ccgataagcg gtcatttttc cacaaaaatt
    22741 tgcaaaattt tgttaaaata caaccgcttg caaaaggacg cattttgcgt ccttttattt
    22801 ttttatgaaa taacaggctt aaagaaaaat gcgatcacaa ttttgctgga aaagtattgc
    22861 tgccctaaca acttttatcg ttttgcttcc tcttattgca atttgctatc acgcattgca
    22921 aggggataat gaaaaccttg ctcacctttg gcaaacaatg ttgccaatct atcttaaaaa
    22981 ctcaacttta ttagtattag gaaccgtttt ccttgcactg atttttgctc tgcctaccgc
    23041 ttggattgtg gcaaattacc gtttttgggg gcaaagaacc ttacaatggt tactttgttt
    23101 gcctctcgct atgcctgctt atttaattgc ctatttatat accgatttat tagattactc
    23161 aggtgctttt caaggctggc tgcgtagtat ttttggctgg actcatccgc aagattactg
    23221 gtttcctcaa attcgtacat tgtatggtgc ttgctttgtg cttgcgttag tactatatcc
    23281 atacattttt ttattagtgc gagttgcctt attagaacaa tctgaaaact taacacacag
    23341 tgcaaaaatg ctcggggctt cgccctctca gttgttccgt cgaattacgc tgccattaat
    23401 tcgaccttct gttgccgtag gcattgcatt agtggcaatg gaaacgcttg gcgatttcgg
    23461 tactgtggcc tattttgccg tgccaacact tactacggca atttatgatt cgtggctagg
    23521 ctatcacgat cttggtactg ccagccagat tgcgatcttt atgttgctaa tgattttcct
    23581 gtttttaagt ttagagcaat atagtcgccg taaacaaaaa agctatcaac gtggctatga
    23641 aaagaaatca gcctttaaat ttttaactgg ttggaaatta gcattagccc ttgcttggtg
    23701 ctggggttta ttggcgttag cctttttcat tccgtttggc aggttgctct attgggcata
    23761 cgactatttc gagcaagcgt ggaatttaga ctttgtccaa ttttcgctta atagtttgaa
    23821 agtatcgatc atcgcctccg tgataacggt actgattgct ctacttctgc atttttctaa
    23881 acgtttgaat acccgcaaaa ttttagtcaa aaatgaccgc ttgtcattac aacttgcctc
    23941 actgggctat gcggttcctg gcacagtatt agcgatcggt ttactttcgc ctttaacatt
    24001 tgccgatcat caactccatg cttggctaaa aagccttgaa ttatcacccg ttggattgat
    24061 tttttcaggc tcactctttg cattagtatt agcttatacc atccgatttt tagcaatggc
    24121 aattggcagc cttgaaactt cgctaaataa aatctctcca tcgctggata tggcgagcca
    24181 aacgcttggt aaaaatggct cagcaatgtt agtaaaaatt catatccctt taatttataa
    24241 aggtatttta actgcaggat taatggtctt tattgagagt atgaaagagc taaatgcttc
    24301 tctgctactt cgcccattta attttgatac actcgcaacc tatgtgttta cctttacatc
    24361 agacgagcag cttgagcgtg ctgctctgcc tgccatcgta ttggtgttag tcggtttact
    24421 gcctgttatt tggcttacca gatcccttat ttcgcaatct gaaaaagaaa gataaaggaa
    24481 ttttatgtct gttttaacta ttaatcatct caacctcaat ttcggcagta caaaggtttt
    24541 acaaaatctc catcttgctg tgaatcaaaa tgaaattatc tgtttgctag gtgcaagtgg
    24601 ctgtgggaaa actacactgc tcaaagccat tgcaggttta ttgccaattg agcagggaga
    24661 tattcattta gcgggtgaat gcctaaaaag taaagccgtg gaagagcgac aaatcgggct
    24721 gattttccaa gattacgctc tttttccaca cttgacggta gccgataata ttcaatttgg
    24781 cttatccaaa ttgccaaaag tacaacaaaa agcaattacc gaaaaaatgc tttcagttgt
    24841 gcatttacaa ggctttgaaa gccgttttcc acacgaactt tcgggcgggc aacaacagcg
    24901 tgtcgctatt gcccgagcat tagcctgcca gccagaattg ctactgttag atgagccgtt
    24961 ttccaatatt gacagccaaa ctcgctatgc aatgattcaa gagatcaagc aaatcttaaa
    25021 aagccaaaat gtgcccgcta tttttgtaac ccatagcaaa gaagaagcct ttgcatttgc
    25081 cgataaaatt gccgtgatgg atcaagggaa aattattcaa tttggtaagc caaacgagct
    25141 ataccattct ccagttaatc catttgtggc agatttttta ggtggcacaa actatcttaa
    25201 ctgtaatgta acggaagaca acgtgttatg cagccctatt ggcgagttcc gactcttccc
    25261 tgaaatgaac gttcagaaag ggcgttaccg ttggcttctt cgcccagaac agattatctt
    25321 aaaacccaat gagctaggtt taggtaaagt ggtaggcaaa ctgtttttag gcctatacta
    25381 ccgctatcag gttgaaatta aaggcattga gatagtaact taccaatctc aagagctgaa
    25441 aattggcgaa caagtggaag tgggcttcca agtaagagag tttatcctat tttaaggaga
    25501 atatatgaaa attgaagttt ggtcagatta tgcctgcccg ttttgttaca tcggcaaacg
    25561 ccacttagag caagccctag cccaatttga aggcgaggtt gaagtggttt tccgagcctt
    25621 tgagcttgat ccgcacgcta acggcgaacc ggagggcgat attcagcaac gcctaatgcg
    25681 gaaatatcaa aaaaccgcag aacaagctga tgaaatgatt cgttatgtgg aacaagccgg
    25741 caaacaagct gggctagatt tgcgttaccg caccacccaa tacactcgca cctttgaagc
    25801 tcatcgcttg gcgaaatttg cagaaagtaa agacttaggc gaagcaatgg ttgaacgttt
    25861 attcaaagcc tattttaccg ataacactat tctagctaaa cgcacagagc ttatcagttt
    25921 agccttagac attggcttag agcgtgatga aatcgctcaa ctgctcacag gtgatgattt
    25981 cgggcacgaa gtccgtgaag atgagcgagt cgctcacaaa tatggcattc acagcgtacc
    26041 gttttttgtg atcaatgaaa aactcggggt atccggggct caaccgccag aaatattact
    26101 tgacgctatc aaacaggcat tgcaaaaata atccgaaaaa agaccgcttg ttaagctaaa
    26161 caagcagtct tattcttctt ataatttaca atttattttt tggtgatcgc acctaaaata
    26221 ccacgcataa tttgtttggt gacttggttg cgtaaatttc tgccaactga atgcgccact
    26281 tggctaacca cttgttcggt aatcgattga tcttttttct tcttcgagcc aaaaatggaa
    26341 gagagaatac cgccaaagaa gtcattgttt gcttcttctg ccgcagcctg ctctgcttta
    26401 attgcttctg ctttcgcatt aagtaattca aacgcagatt cattatcgac atattggctg
    26461 tagtgatgat agagatcatc ttgcttcact aaactatcac gttcgcctgt ggctaatgga
    26521 gctaattggc tttttggtgg atagatatag gcaatttcta caggcgttgg catacctttt
    26581 tcatctagca ccgaaaccaa cgcttgcccc acaccaagtt gcgtgatggc ttctaccaca
    26641 tttacctctt tattcgctcg gaaagtttcc gctgctgatt ttaccgcttt ttgatcacgt
    26701 ggcgtgaaag cacgcaaagc gtgttggata cgattgccaa gctgccctaa tactgaatca
    26761 ggtatgtcca acggattttg cgtaacaaaa taaacaccca ccccttttga gcgaattaaa
    26821 cgaaccacct gttcaatttt atccaccaat gccttagggg catcgtcaaa caataaatga
    26881 gcttcatcaa agaataaaac aaacgtcggt ttttctgggt cgccaacttc tggcaaattc
    26941 tcaaagagct ctgccataaa ccaaagcagg aatgcactgt acactcttgg tgagttaatt
    27001 aatttttctg cattcaacat attgattaca ccacgcccat tacgggtttg gatccaatcg
    27061 tgcaaatcta atgcaggctc accaaataaa ttatctgccc cttcattttc aagccttaat
    27121 aacgcacgct gaatagctcc aacgcttgct gcagaaatat gcccatattc cagttggaat
    27181 tctttcgcat tatctgcgac aaatttaagc aacgcacgca aatcttttaa atcaattagc
    27241 aataaacctc tgtcatctgc cacacgaaaa acgagattta acaagcctcc ttgggtatcg
    27301 tttaaattga gtaaacgaga aagtaacatc ggtcccattt cggaaatagt gattcgaagt
    27361 gggatacctg atgtaccaaa tacgtcccaa aacgaaactg gatagccctt taagtaatct
    27421 tctcccccta aattaaactg taaaatgcgt tctgcaattt ttcctgaaaa attacccgct
    27481 tgtaccgagc ccgaaagatc accttttaca tctactaaaa atactggcac gccatcatca
    27541 ctcaaagatt ctgccaattt gcgtaaagta accgttttac ctgtacccgt agccccagca
    27601 attaaaccgt gccgatttgc cattttggca ttaagcgaaa taactttgcc tgtttctgtt
    27661 cgagcaattt gatacaaact catctttcat tctcttaaag gtaaattaat tttttgttct
    27721 catcatataa aaatttatca cttaattacg gctattcttt ttcgtttttg ccaagaacgt
    27781 ttttttactt cttgagcggt tcactttcgg taaagtcagt aaggattcct tgtcgtattg
    27841 actcacagga atctgatgcc caatatactc ttcaattgca ggcaaattaa ccgcataacg
    27901 ctcgcaagca aaactaattg aatggccgct ctcccctgct cgccccgttc taccaatacg
    27961 atgaacgtaa tcttctttat catcaggcag atcataattg aaaacgtgcg tgacatctgg
    28021 aatatgcaat ccacgagccg caacatctgt taccactaaa atatccaaat tgcctttggt
    28081 gaaattatcc agcaaagaaa ggcgtttttt ctgcggaata tcgccggtta gcattccaac
    28141 ccgatgtccg tctgccgata aatactgcca gatctcttcg catttatgct tggtgttggc
    28201 aaaaatcata caacgctccg gccactcttc ctcaagcaat gtcagcaaca atgccatttt
    28261 gtcttcattc gaggggtaaa atagctcttc tttaatacga tgtccggttt tttgtagtgg
    28321 ctcaatttca atatattccg cattatccat atcttcatag gcaagttccc gcacttttgc
    28381 cgaaagcgtt gccgaaaaga gcaaagttaa acgttctgaa ggcttaggtg cttttcgcat
    28441 taaataacgg atatctttaa taaaaccaag atcaaacatt cgatctgctt catctagcac
    28501 aatcacttga attttgtcta aattgataat gccttgtttt acatagtcaa tcactcgacc
    28561 tgttgtaccg acaagaatat ctaccccttt ttcaatttct tgaacttgtt tgtcgtaacc
    28621 atcaccaccg tatgccaaag ctagttttaa atcagaatgt tttaaaaaca gctcggcatc
    28681 tgccccaatt tgaaccgcca attcacgggt tggagcaaga attaatgctc tcggctgatt
    28741 ttttgacgtt tcgatttcgt tattcaataa atggttaaaa gttgcaatca aaaaggcaag
    28801 cgttttccct gtacctgttt gagcttgccc tgcaatatct ttgcctttca gcgtaatcgg
    28861 aaaggttttt gcctgaatcg gcgtacaaaa ttcaaagcct ttgctatcaa gggcatttag
    28921 tacattagcg tgaatcggga agtcaataaa gcggtgttcg gtaagatgat tagacatagt
    28981 atttccttga taattgtgat gtgacaagcg gtctttttta cacatttttt tgcaaaatag
    29041 catattgggc gttttttctc aaatttaacc gcttttgagt ttattcggca aaatagattg
    29101 gaaaagctct cggcttttca gcggttgtga ccctaaatag ggctttagtg ccattcgggt
    29161 aaaacgtttc gctgcttgtt gagtactttt ttctgaaaaa tccaactgag caaacgctaa
    29221 taaatcactg cctaaaaagc tctggttatt ctgtaataat gaggcgataa agccctgatt
    29281 ttcctgaaat tgataggtca ttttcggatc aactggcaaa ccggttgccg cacagtgcgt
    29341 aaaatccacc ccataaccca atgcttttaa cgtatgaaat tcaaaggtgc gtaaaatcgg
    29401 ctcaatctgt ttttgtgtgg caagctcggt aatgcaacgt aaataatgct gaaataattc
    29461 agggtaagcg gtctgatttt ccaatactct tgcaagtacc tcgttgatat aaaacccact
    29521 ataaagtgaa agcgtttcca tcggcaacgt taatgcagcc ggttctgctt tggttaaagt
    29581 tttcagatcc cctttacccg accaacgcaa caacaacggc gtgaacggct gtaaaaccgc
    29641 tttcaacggc gagcgagccc gccttgcacc tttagcaagt aaagtaatcc gcccgtgatg
    29701 ttcggtaaaa aactccacta ataaactgtt ttcgctgtat tcacgccgat gaaggacaaa
    29761 ggctcgttgc cattggtcta ttgtcataat ttcttgttaa ttatgaggtt gtaatttact
    29821 tctgccaaat ctccccctcc cctctttgct aaa
//
LOCUS       DANLZC010000010        29580 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.010, whole genome shotgun sequence.
ACCESSION   DANLZC010000010 DANLZC010000000
VERSION     DANLZC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 29580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29580
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.010"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(11..631)
                     /locus_tag="PXE44_000380"
     CDS             complement(11..631)
                     /locus_tag="PXE44_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDL5587926.1"
                     /translation="MCRFPLFETLSIIDGKVQNLQYHQQRFEQATQEYFGCEPRFSLA
                     DILVVPEASQRGKVRCRIDYNATEFELKFFIYSPKKVERFRLVEVENWDYHLKFSDRK
                     RFDLLPILQNEEVIILNNGYVSDCSIGNLLFLKQGIWYSPQHYLLKGTQLSKLLDEGK
                     VVLAKIAKQDLHQYEKIMLINALNPFEEPRALPCSQIYFCDLDRKS"
     gene            complement(609..1583)
                     /locus_tag="PXE44_000381"
     CDS             complement(609..1583)
                     /locus_tag="PXE44_000381"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDL5587927.1"
                     /translation="MQDFIHQANHYGQAKRPFFFLIDFEQQKPLIFPLEQAHASGLFF
                     EFFCKSNLEKPVEKPTKAFELSANPIPFSEYQTGFELVKKEIQAGNSYLLNLSYPTAI
                     ETNYSLAEIFAASKAKYKCYLQGRFVCFSPECFVRIENNRIFSYPMKGTINANEENAE
                     QKLMASEKEFTEHNTIVDLIRNDLALVAKNIEVTKYRYVEKVETHRGAILQTSSEICG
                     ELGENWQENIGSILAKLLPAGSISGAPKEKTVSIIQQAELGKRGYYTGIFGYFDGESL
                     ESAVAIRYIAQTSNGLVFHSGGGITSQSVLTDEYNELLEKVYVPISAI"
     gene            1693..3300
                     /gene="rmuC"
                     /locus_tag="PXE44_000382"
     CDS             1693..3300
                     /gene="rmuC"
                     /locus_tag="PXE44_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDL5587928.1"
                     /translation="MHFQEIGIYIALAVCVLVGVYLFFLKSRYQRNAYELSQDLGKIT
                     ENFEQIQQKYEVLAQEKNQLEQWAIQQQTKYEAVSERLNERDGQLQRFQQKIELAEQQ
                     ENQLERYINELKERVGSSQAKAESLEEQLQFSQSSLSTKERENQALFSRLNEVQNELT
                     ELRTTLSEKQANFEAQQRNFIEVKQQLNVEFQHLAQQILDEKSKRFSETNQSSLEALL
                     KPFKEQIEGFQKRVNEVHSESLKGSANLEAEIKRVLQIGVSMSEEAQNLATALKGNNK
                     IAGNWGEVQLESALQSAGLLAGEHYQAQESFRDEEGRRFAPDFVVHLPDQKHLIIDSK
                     VSLVAYDQAVRSEENFAISQALDEHCRSLRNHIEGLSKKNYSALLGIKSPDFVLMFIP
                     IEPAYIEAMKHDPQLFNFGYERNVILVSHTTLMPILRTVANLWRIERGNSEAREISEK
                     AGEIYNQVCAVADRLAKLGNTLNTANNQYNQTVTSLVGRQGLLGKVERFQHLSSKASQ
                     IVPQVELIENEVDTIKLEMVSRELLEE"
     gene            3363..3947
                     /locus_tag="PXE44_000383"
     CDS             3363..3947
                     /locus_tag="PXE44_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HDL5587929.1"
                     /translation="MLKKLLIIDNHDSFTFNLVDLLRKLDVPAKVVLVEELDLNEVEH
                     FSHILISPGPDVPRAYLQTFAMLERYHQTKSILGVCLGHQTICEFFGGKLYNLNDVRH
                     GQQRTLTQIEPNPIFKGLPKQLKVGLYHSWGILQNSLQNTPLVPTAVCDQNVLMAFKH
                     QHLPIFGVQFHPESFITEFGQQMLANWLEITQDF"
     gene            4044..5126
                     /gene="prfA"
                     /locus_tag="PXE44_000384"
     CDS             4044..5126
                     /gene="prfA"
                     /locus_tag="PXE44_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL5587930.1"
                     /translation="MKDSIINKLESLSERHEELQALLGDASIISDQDKFRAYSKEYSQ
                     LEEVVGTFNRWKKLNNDIEEAQLLLDDPDMKDMAAEEIAENKAEIENLEQHLQILLLP
                     KDPNDEYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIEEMSANESEQGGYK
                     EIIVKISGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEIN
                     PSDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNKAKALAVLAS
                     RIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMN
                     GKIDELIQPIITEYQADQLAALSEQN"
     gene            5238..5840
                     /locus_tag="PXE44_000385"
     CDS             5238..5840
                     /locus_tag="PXE44_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587931.1"
                     /translation="MRKLGLLAVFTLVLSACSNSTKIEQTILSKQDIVGSWVCAIKYD
                     DLKVSTLDFSEFKANGEMTNFGDISDENFEPIKFTYVTGDKGKWDLKGNRLVIDYDLS
                     ERKVKRTTPKKFLEFLKQEERKASKTAKKLLKYEQGIFNILSDQKNTENSKIILDILK
                     FGHSRMAIQQNMGDKVYSGGCVTADKAEEYLDILKNRNSK"
     gene            5840..6706
                     /gene="prmC"
                     /locus_tag="PXE44_000386"
     CDS             5840..6706
                     /gene="prmC"
                     /locus_tag="PXE44_000386"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL5587932.1"
                     /translation="MDYREWLTFAEQKLLENSGADPYLNAKFDANLLLQAVTKRSKSA
                     IFAFSETLLSESELAELAEKLARRAKGEPMAYILGYREFWSLPLKVSPATLIPRPDTE
                     RLVELALEYANKRLGNQKMLQILDLGTGTGAIALALASELGEKAQIIGVDFQNEAVQL
                     AEENRRNLGLNQVTFLQSDWFSSLANQQFDLIVSNPPYIDESDENLTVGDVRFEPLTA
                     LVADNKGLSDLQKIIENAPLHLKPQGALMLEHGWKQGNLVRDLFDLHLWEQVETAQDY
                     AGNDRVTKAILK"
     gene            6715..7581
                     /locus_tag="PXE44_000387"
     CDS             6715..7581
                     /locus_tag="PXE44_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HDL5587933.1"
                     /translation="MDDIIEELLKELEDPKVTISETELKKFLYREILRLTTLIDKNVS
                     EQQVFGQMSALVKKARYNVNGEGDEERINQLLHLVYREWGFHCYYEDYFHTENLLLNQ
                     VIRKRRGMPVSLGAVALYLSAVLDLPLYPVNFPTQLVLRAEIHQPNGATKVRFINPWN
                     GEFLTIEMLNKWLEGEMGFGVEVTPDLLRRAEPQELLERVETVFKMALTREGKYEETL
                     RLIEYRLAFSPEDPYEIRDRGMVLASMDCYQAALEDINYFIDQCPEDPSAEILKMEVK
                     GLQQKSQESVVH"
     gene            7671..8525
                     /gene="kdsA"
                     /locus_tag="PXE44_000388"
     CDS             7671..8525
                     /gene="kdsA"
                     /locus_tag="PXE44_000388"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDL5587934.1"
                     /translation="MTDKIVKVGNIEVANHKPFTLFGGMNVLESRDMALRVCEQYVEV
                     TQKLNVPYVFKASFDKANRSSIHSYRGPGMEEGLKIFQELKQTFGVNIITDVHEIYQC
                     KPVAEVVDVIQLPAFLARQTDLVEAMARTGAVINVKKPQFLSPGQMGNIVEKIAECGN
                     DQVILCDRGTNFGYDNLVVDMLGFGIMKKVSKGCPVIFDVTHSLQCRDPFGAASGGRR
                     DQVTELARSGMAIGLAGLFLEAHPDPNSAKCDGPSALPLSKLEAFVSQMKAIDELVKS
                     FEEIDTSK"
     gene            8746..9693
                     /locus_tag="PXE44_000389"
     CDS             8746..9693
                     /locus_tag="PXE44_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HDL5587935.1"
                     /translation="MLNIVFLDRTGIPATHDIPRPNFPHTWTEYATTSADQTFERVKE
                     ADIIITSKVLLQRDLLAKLPKLKLIAITATGTNNVDLAAAKELGIAVKNVTGYSSVTV
                     PEHVIGMIYSLKHRLADYHRDLITSDRWATCGQFCYVDYPIHDVRGSTLGIFGKGNIG
                     AEVARLAQAVGMQVIFAEHQGATEIREGYTAFEEVFKQADIISLHCPLTEQTQNLINA
                     ETLALMKPTAYIINTGRGPLIDEQALLEALEQGKIAGAALDVLVKEPPEKDNPLMLAA
                     KRLPNLLITPHVAWASDSAVTTLVNKVTQNMEEFVATGM"
     gene            9791..10963
                     /locus_tag="PXE44_000390"
     CDS             9791..10963
                     /locus_tag="PXE44_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HDL5587936.1"
                     /translation="MNLTPFFIAFRYFRSKSADRFGRLVTNLASLGIVLGVMALVIVL
                     SVMNGLENMQKTNLLSTLPHAIITPQEGHFAKGEKLALPEFAKQSVVINRANVIIQSS
                     QGINAGQLIGVENATDDPLLVAENIPALLPSGEFKVVIGSRLANKLNVTVGDKLRLMI
                     TENSQYTPLGRVPVQRLFEISGVYQSNREASEFTLFANLADVGRLLRISEDQVQGVRL
                     YLQDPFQVTELSQYFDAERYQISDWREQKGEFFQAVKMEKNMMGLLISLIIVVAISNI
                     VTSLSLMVVDKQGEIAILQTQGLTKKQVMQIFVFQGAIVGVIGSIIGGLLGVLIAYNL
                     DQIILLLNPSIHLPTLISGTQIAVIVGVSMLLSLLCTLYPAYRASKIEPAQALRYE"
     gene            10980..11663
                     /gene="lolD"
                     /locus_tag="PXE44_000391"
     CDS             10980..11663
                     /gene="lolD"
                     /locus_tag="PXE44_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDL5587937.1"
                     /translation="MTELLRCEHISKFYDEGEQKVQVLKEVSFSMNEGELVAIVGSSG
                     SGKSTLLHTLGGLDQPSTGEVWIRGQSLQQLSSDKLALLRNQNLGFVYQFHHLMADFT
                     ALENVMMPMLIGKQNKTEAANRAEKMLQAVGLAHRMTHRPSALSGGERQRVAIARALV
                     NNPALVLADEPTGNLDQKTTESIFELIQQLNQEQNIAFLLVTHDLNLANKLSRSLVMR
                     DGVLSEGSL"
     gene            11660..12925
                     /gene="lolE"
                     /locus_tag="PXE44_000392"
     CDS             11660..12925
                     /gene="lolE"
                     /locus_tag="PXE44_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDL5587938.1"
                     /translation="MSTPFFISWRYQRGKQKNKLVSLIALFSNIGIALGVAVLIIGLS
                     AMNGFERELNNRVLSVVPHAELVSFQGNQPQPIFDSKKLEALIKKSQNVTASSPFVSF
                     TALIENGAQLKIAQVRGVDPKKQDQASQLSQFIPPEQWQAFNQQFQADEDGLILGAGI
                     AKALEVEAGDEVTLLIPQPTEDGKLAQPLRFNLNVTGILRLDGQLDHSYALIPLNKAQ
                     ELMEYQANEVSGLEISLANPFDVQQLEMPQLNGYTQPLYLNTWIDKFGYMYNDIQLIR
                     TVMYIAMVLVIGVACFNIISTLIMAVKDKQGDIAILRTLGANNGFIRRIFLWYGLISG
                     MKGALFGLILGVILSLNLTAIIKAIEAFFEVKLLSDGVYFVDFLPSELHWQDVVYVLA
                     ATIILSLFASLYPANRAAKLEPAKVLSGH"
     gene            complement(13000..14931)
                     /gene="thrS"
                     /locus_tag="PXE44_000393"
     CDS             complement(13000..14931)
                     /gene="thrS"
                     /locus_tag="PXE44_000393"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL5587939.1"
                     /translation="MPIITLPDGSQRSFDNPVSVMEVAQSIGAGLAKATIAGRVNGER
                     RDASDIISQDSTLEIITAKDEDGLEIIRHSTAHLLGHSIKQLFPDVKMAIGPTIENGF
                     YYDIDLDRSLTQEDLDALEKRMLELAKTNYDVVKRPVSWQEARDTFEKRGEPYKMAIL
                     DENIERTATPALYHHEEYIDMCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRI
                     YGTAWADKKQLAEYLQRLEEAAKRDHRRIGKALDLYHMQEEAPGMVFWHNDGWTIFRE
                     LETFVRTKLKEYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTTQSENREYAIKPMNC
                     PGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IESEVISCIKMVYDIYSTFGFTNIAVKLSTRPENRIGDDAMWDRAEKGLANALRNNGL
                     EYEIQEGEGAFYGPKIEFALRDCLGREWQCGTVQLDFALPGRLAASYVAEDNGRRTPV
                     MIHRAILGSIERFIGIITEEYAGFFPTWLAPTQAVVMNITDSQADYVQQVVKQLSDAG
                     IRVKADLRNEKVGFKVREHTLRRVPYMLVCGDKEIAEGKVSVRTRKGADLGTYLVSDL
                     VEILKNQIKARELKLLGEE"
     gene            complement(15098..16447)
                     /locus_tag="PXE44_000394"
     CDS             complement(15098..16447)
                     /locus_tag="PXE44_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5587940.1"
                     /translation="MNSTKFYSLYSGIFFLFFGYGLFLNSAGIKLAEMGITDIIIGLL
                     NAAFFIGAALSTIFAHRIVSSVGHIRSFSVFCAVFAIAALGHIMIDTLWIWGVLRIIL
                     GFCHYSLLLLVESWLSEKTNVDTRGKVLATYNIIFYLAFILGVSLLSLELSSRNIFIL
                     STILVVMSMIPIALTKMVQPDIPARERISIPKLFAISPLALATSFISGMLVNGFFTMA
                     SVFLLKFSFSLSEISLYLMISMIGGFISQMPMAILSNRIGRRNAILACSVIATLTALA
                     GLFAIFQENLTAWGQYIIAFFFGCSLFSLYALSIARANDELPNNMNTVEVSRGLLFCY
                     GVGALVAPPLIGFAMSLSAEYGFYALFGLFSLSLFICAFRTKSIPKAERAEMQYATPI
                     ATASPLEEIEVVEDALIPFDEEAVQEYQAQLEILEQDPQTENENIELDEGAAENSKV"
     gene            complement(16722..17213)
                     /locus_tag="PXE44_000395"
     CDS             complement(16722..17213)
                     /locus_tag="PXE44_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL5587941.1"
                     /translation="MFTTQLIQTKRWLEANSITTFWLKTFIGATLLAIFAQISVPMYP
                     VPITGQTLALTVVGFAMGRKAATAAVLLYLFEGAIGLPVFAGGASGFHHFFGPTGGYL
                     LGYIPTAYFLGYYSDKGVLNSFWKSLFVALIASVITFAFGLAQLSLYQKAQYYKRAYI
                     HLF"
     gene            17294..18061
                     /locus_tag="PXE44_000396"
     CDS             17294..18061
                     /locus_tag="PXE44_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HDL5587942.1"
                     /translation="MVKPVYIYCDEGSSEIGVQSLLLAVKQHLQRPAYLINAKQIIQS
                     KFSDGCLLIMPGGADLPYCKKLNGEGNRRIRQFIEQGGAYLGICAGAYYGCAELNFVG
                     RDYSVNGNRELALFNGIAGIAKGSLPQFTNGQLYDKNIGSKAIVEVTLAEGGKMPFYY
                     HGGCCFEPNSTTVIYNPLAYYPDGSVAMIEGELGRGYYLLSGVHFELQAVVYWQKIVN
                     CCENLDIKTQEEAICSNFNVNYGSQIWQHLKQRLNQA"
     gene            complement(18052..18846)
                     /locus_tag="PXE44_000397"
     CDS             complement(18052..18846)
                     /locus_tag="PXE44_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL5587943.1"
                     /translation="MRFFDTHTHLDYLAESLNLSIPELVENAKQHQVECILIVAVFAK
                     NFAKVTACAKQKPENLVYGLGLHPLYIQQHQQQDLILLENLLSQQDPQCTAIAEIGLE
                     KAVSEVSTPELWKKQCEFLEAQLSLAKKFNLPVNLHSRKSHDQLFVFLKKAQLTKTGV
                     VHGFAGSYEQAKRFVDLGYKIGVGGTITYERANKTREAIRKLPLESLLLETDSPDMPV
                     FGFQGQPNRPERLLVTFNSLCELRTESPNLIAETIWQNSERLFFYA"
     gene            complement(18907..20493)
                     /locus_tag="PXE44_000398"
     CDS             complement(18907..20493)
                     /locus_tag="PXE44_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDL5587944.1"
                     /translation="MLWFFFCVAVLVLGYFTYGKIIEKIFVINPKRATPAYAMNDGVD
                     YMPMSKTKIWLIQLLNIAGTGPIFGPILGALYGPVAMLWIVIGCIFAGAVHDYFCGML
                     SIRNGGATMPNLAGKFLGRPVKLFINVMALILLLLVGVVFVASPAQLLSTITMDIAGA
                     TQSAMELGDVEAIKESVASGGVTVWGMDKATVISVWTLIIFAYYILATLLPVDKIIGR
                     IYPFFGALLLFMSVGMVYGLVSAHLSATDPIDFFRTVNADGQGLTWEKFTQNFQVKGD
                     VPIWPLLFLTISCGALSGFHATQTPLMARCAQNEKEGRFIFYGAMITEGVIALVWCAV
                     GLSFYENPQALQDAISAGSPSKVVYDSSLHFLGFIGGIFAVLGVVVLPITSGDTAFRA
                     ARLQLAEIFGLEQRTLVKRLYIAVPLFVLGFIVSKVDFSVLWRYFTWANQMTAMVMLW
                     TAAGYLYRYKKFHWVCSIPAWFITTVCATYLANNKIGFGLDYELSVYIGFAITVVCVI
                     CFFAFLKPSEARDEDAYVTN"
     gene            complement(20679..22532)
                     /gene="dxs"
                     /locus_tag="PXE44_000399"
     CDS             complement(20679..22532)
                     /gene="dxs"
                     /locus_tag="PXE44_000399"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL5587945.1"
                     /translation="MEKKYPLLSEINSPEDLRLLSKDQLQTVVDELRAYLLESVSQTS
                     GHLASGLGVVELTVALHYVYQTPFDQLIWDVGHQAYPHKILTGRRDQMHTIRQKDGIH
                     PFPWREESPYDVLSVGHSSTSISAGLGIAVAAEKENAGRKTVCVIGDGAITAGMAFEA
                     LNHAGALHTDMLVILNDNEMSISENVGALNNHLARIFSGSLYTSVRDGSKKILDKVPT
                     IKNFMKKSEEHMKGVIFSPESTLFEELGFNYIGPIDGHNIDELVKTLSNMRELKGPQF
                     LHIRTKKGKGYTPAENDPIGYHGVPKFDPTAAELPKSKAAPTYSNIFGDWLCEMAERD
                     PKIIGITPAMREGSGMVEFSKRFPEQYFDVAIAEQHAVTFGAGLAIAGYKPVVAIYSS
                     FLQRAYDQLIHDVAIQNLPVIFAIDRAGIVGADGQTHQGAFDLSFMRCIPNMTIMAPS
                     DENEMRKMLYTAYTMNTPSAIRYPRGNAKGVKLEPMQRLEVGKGNIIREGKKVAILNF
                     GALLDEAKIVAEKHNYTLVDMRFIKPLDEALLQKVADSHELLVTLEENAIQGGAGSFV
                     NEYLQNIGKIKPLVMLGIPDFFVPQSTQAEAYADLGLDAQGIEAKIQKFDI"
     gene            complement(22577..23389)
                     /gene="suhB"
                     /locus_tag="PXE44_000400"
     CDS             complement(22577..23389)
                     /gene="suhB"
                     /locus_tag="PXE44_000400"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDL5587946.1"
                     /translation="MNPMLNIAIRAARKAGNIIAKGYEQSPQDTQVLQKNTNDYVTEI
                     DKAAEAAIIDVIRKSYPEHSIVTEESGVLNGSNENVQWVIDPLDGTTNFVKRLPHFAV
                     SIAVRENGRTTVGVVYDPIRNELFTAVRGEGAKLNEFRLRIDAERRDLKGTVIATGFP
                     FKVIRHRDAHLNMLEGLMNNGVADFRRTGSAALDLAYVAAGRVDGFFEIGLKPWDFAA
                     GELIAREAGAIVTNFVGGTDYLKTGNLVVGNGRVVKEILNSIQPTLTEELKA"
     gene            23570..24604
                     /locus_tag="PXE44_000401"
     CDS             23570..24604
                     /locus_tag="PXE44_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HDL5587947.1"
                     /translation="MQFKENNQPRFKHREERRSDECSPRFSDKGKMRGNEGKSDRPHK
                     LHFKRDEAYSRSEQIYPAEGKKASGEGSVQIWVKGGNSSVKEKKNGPLSPRAPEKIKK
                     NRLEEMKVYGENACISLFQQRPESIVRLWATVEGAKKLGDLMSYLAQHKKAYHVIDRE
                     EMERVTGTEHHGDMCLLVKKTASFVLEGYLQVPKKQDCLVLLDGVNNAQNVGGIIRTC
                     AFYGVKGIITENVDSLNSSAAARVAEGGLEFVRALETKNKHIALVQLRQAGYQIVHLT
                     RHKQAPSLTKIKLAEKVVFVLSEIPASDVAYAEDTTIQLSFNNPLNSGLNVAVNAGIL
                     LNQWYQTHIL"
     gene            24614..25498
                     /locus_tag="PXE44_000402"
     CDS             24614..25498
                     /locus_tag="PXE44_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDL5587948.1"
                     /translation="MNQQKPFEVIAIVGTPRHDSAFETHLAVYNWLKDRDYQILVETE
                     VATQLQLKESYSLDQIGQRAELVIVIGGDGNMLGIARALAKYRVPLIGINRGNLGFLT
                     DIAPQTAFEQLYSCLVKNEYVIEERFLLEAFIERNGKIIAANNALNEIVVHPTQVARI
                     IEFEVYIDGKFAFSQRADGLIVATPTGSTAYSLSAGGPILTPNMNAMALVPMHPHTLS
                     SRPLVVDGDSQISLRFAKYNTPDLEVICDGQVNMHFTPEDRVLVRKSPDKLQLLHLKD
                     YSYFTVLGSKLGWASKLF"
     gene            25637..26614
                     /locus_tag="PXE44_000403"
     CDS             25637..26614
                     /locus_tag="PXE44_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1852 domain-containing protein"
                     /protein_id="HDL5587949.1"
                     /translation="MTTNFKCQVHTIEFNEDYQPEQSTRLTTNFANLARGENRKQNLQ
                     NAIAMINNRFNDLANWDNPNADRYSVKLDIVSLDIDVEGKGEFFPAIEWLKTYVIDYK
                     TGKTIDGIIGNNFSSFVRDYDFSVLLLDHNKDKPQFSVPEGYGELHGKLFKHLLASPE
                     YTAKYKKPPVICLSVATTRTYTRTTNVHPILGVEYLQDSYSLTDAYFAKMGLKVRYFM
                     PPKSVAPYAFYFDENADLLNDYTNLELISTIATMEAFQKIYRPEIYNANSVAGQVYKG
                     SLTYQDYSVTKVEYDREERTQLAIKQGKYTEEHFIKRYQAILEAWATEE"
     gene            26702..27733
                     /locus_tag="PXE44_000404"
     CDS             26702..27733
                     /locus_tag="PXE44_000404"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL5587950.1"
                     /translation="MSNILLPTSTAGSLPKPSWLAEPEKLWSPWKFEGEELLQAKRDA
                     LLVSLAEQIAAGIDIVSDGEQTRQHFVTTFIEHLEGVDFSKREIVRIRNRYDASVPSV
                     VGAVSRPKSVFVDDAKFLRAHTDKPIKWALPGPMTMIDTLYDGHYKSREKLAWEFAKI
                     LNEEAKELEAAGVDIIQFDEPAFNVFFDELNDWGVAALERAAEGLKCETAVHICYGYG
                     IKANTDWKQTLGNEWRQYEESFPKLQQSKIDIISLECQNSRVPMDLIELVRGKKVMLG
                     AIDVATNIIETPEQVADTLRKALQFVDADKLYPSTNCGMAPLSRQVAQGKLQALSQGA
                     AILRAEFAK"
     gene            27821..28042
                     /locus_tag="PXE44_000405"
     CDS             27821..28042
                     /locus_tag="PXE44_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5587951.1"
                     /translation="MLNLFKKPIKKSSFEAWAKLMDDVAKVAILAIPVILYGEYSLLM
                     KFTHSITLFAVVYFLLATARLFRETAEEM"
     gene            28318..29580
                     /locus_tag="PXE44_000406"
     CDS             28318..29580
                     /locus_tag="PXE44_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL5587952.1"
                     /translation="MLKRDMNIADYDAVLWQAIQDEDRRQEEHIELIASENYASPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKALFGADYANVQPHSGSQAN
                     AAVYMALLNPGDTILGMSLAHGGHLTHGASVSFSGKIYKAEQYGITDEGLIDYEALRK
                     QAQEVKPKMIVAGFSAYSQVMDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPNPLP
                     HAHVVTTTTHKTLAGPRGGLILSASGDEEMYKKLQSAVFPAGQGGPLMHVIAAKAVCF
                     KEALEPEFKAYQQQVVKNAKAMVEVFKQRGFDVVSNGTENHLFLVSFVKQGLTGKAAD
                     AALGAANITVNKNSVPNDPQKPFVTSGIRVGTPSVTRRGFKEAEVTALAGWMCDVLDS
                     IGKDNHEQVIAETKAKVLDICKRLPVYA"
BASE COUNT         9032 a         5967 c         6415 g         8166 t
ORIGIN      
        1 aagcattttt ttaggatttt cgatcaagat cacaaaaata aatttgcgag caaggcaatg
       61 cccttggttc ctcaaaggga ttaagagcat taatcaacat aattttttca tattgatgca
      121 aatcttgctt ggctattttg gctaaaacga ctttcccttc atcaagcaat ttactaagct
      181 gagtgccttt gagcaaataa tgttgcgggc tataccagat tccttgtttt aggaacagta
      241 aattgccgat ggagcaatca ctgacgtagc cattattgag aataatcacc tcttcatttt
      301 gtaaaatagg gagcaaatca aaccgcttgc ggtcgctaaa tttgaggtga taatcccaat
      361 tttccacctc aaccaagcga aaacgttcta cttttttggg cgaatagata aaaaatttca
      421 gctcaaattc tgttgcgttg taatctattc ggcaacgtac tttgccccgt tgagaagcct
      481 ctggcacaac taaaatatca gcaaggctaa aacgaggttc acagccaaaa tattcttgtg
      541 ttgcttgctc aaaacgttgt tgatgatact gtaagttctg aactttgccg tcaataatcg
      601 aaagggtttc aaatagcgga aatcggcaca tagactttct ctaacagttc attatattca
      661 tcagttaaca cactttgtga ggtaatgcca ccaccactat gaaacacaag cccgtttgag
      721 gtttgagcaa tgtaacgaat tgccaccgca ctttctaagc tctcgccatc aaaataacca
      781 aaaataccgg tgtagtaacc ccgttttcct aattctgctt gctgaataat cgaaaccgtt
      841 ttctctttcg gtgcaccgct aatggagccg gcaggtagta attttgccaa tatcgaaccg
      901 atattttctt gccaattttc acctaattcg ccacagatct cggaacttgt ctgcaaaatt
      961 gcccctcgat gggtttctac tttttccaca taacggtatt tagtaacttc aatatttttc
     1021 gccaccaacg ccaagtcatt tcggatcaaa tccacaatgg tattatgttc ggtaaactct
     1081 ttttccgaag ccattaattt ctgctcggca ttctcctcat ttgcattaat tgtgcctttc
     1141 atcgggtagg aaaaaatgcg gttattttca attcgcacaa agcattcagg ggaaaaacag
     1201 acgaacctgc cttgcaaata acatttatat tttgctttgc tggcggcaaa aatttccgcc
     1261 aatgaataat tggtttcaat ggcagtcggg tagctcaaat tcagcaagta gctattgccg
     1321 gcttgaattt cctttttcac taattcaaac ccggtttggt attcgctaaa cggaatcgga
     1381 tttgccgata attcaaaagc tttagtcggt ttttcaaccg gcttttcgag attcgatttg
     1441 caaaaaaatt caaaaaaaag accgcttgcg tgagcctgtt ctagcggaaa aataagtggc
     1501 ttttgctgct caaaatcaat taaaaagaaa aaagggcgtt ttgcttgccc gtagtgattg
     1561 gcttggtgaa taaaatcttg catagaaaac acaatggctg aaattggctg aatcatcaaa
     1621 agctctgtta taatgcaaag caatagattt gaaaagcatt ttttaaaaaa ggttttaccg
     1681 atgaacaaca ccattcattt tcaagaaatt gggatctaca tcgcccttgc cgtttgtgtg
     1741 ctggtaggcg tttatctctt tttcttgaaa tcccgttatc agcgaaatgc ctacgaatta
     1801 agccaagatc ttggcaaaat caccgaaaat tttgaacaaa ttcagcaaaa atatgaggtg
     1861 ttggctcaag aaaaaaatca gcttgagcag tgggcaattc aacaacagac caaatatgaa
     1921 gcagtgagtg aacgtttaaa cgagcgagat gggcaattgc aacgattcca acaaaaaatc
     1981 gaacttgccg aacagcaaga aaaccagctg gaacgctata ttaatgagct aaaagaacgt
     2041 gtgggttctt cccaagcaaa ggcagaaagt ttggaagaac agctacaatt tagccaaagt
     2101 tcactctcaa ctaaagagcg ggaaaatcaa gcgttattta gccgtttaaa cgaggtacaa
     2161 aacgagctga ccgaactgcg tactacgctt tcggaaaaac aggcaaattt tgaagcacaa
     2221 caacgcaatt ttattgaggt aaaacaacag cttaatgtgg aatttcaaca tttggcacaa
     2281 caaattcttg acgaaaaaag caagcgtttt agcgaaacca atcaaagctc gttagaagct
     2341 ctactgaagc cgttcaaaga gcaaattgaa ggctttcaaa aacgggtgaa tgaggtacat
     2401 agtgagtcgc tcaaaggctc ggcaaattta gaggctgaaa ttaagcgggt gctacaaatt
     2461 ggcgtttcaa tgtcggaaga ggcacaaaat ctggcaacgg cattaaaagg caataataaa
     2521 attgccggca actggggtga agtacagctt gaaagtgcct tacaatctgc cggtttactc
     2581 gcaggcgagc attaccaagc ccaagagagt tttcgtgatg aagaggggcg acgttttgcc
     2641 cccgattttg tggtgcattt gccggatcaa aaacatctga ttattgacag taaagtctcg
     2701 ttagtagcgt acgatcaagc ggtcagatca gaagaaaatt ttgcaatttc tcaagcctta
     2761 gacgaacatt gccgttcgct acgcaatcat attgagggat tgtctaagaa gaactacagt
     2821 gcattattag gcattaaaag cccggatttt gtactgatgt ttattccgat agaaccggct
     2881 tatattgaag cgatgaagca cgatccgcaa ctgtttaact ttggctatga acgcaatgtg
     2941 attttggttt ctcacaccac cttaatgccg attttacgca cggtcgccaa tttatggcgg
     3001 attgaacgag gcaacagcga ggctcgtgaa atcagcgaaa aagccggcga aatttacaac
     3061 caagtgtgtg cggttgccga tcggttagct aaattaggta acacgctaaa taccgcaaat
     3121 aatcaataca atcaaaccgt tacctcgtta gtgggacggc aaggcttact gggcaaagtg
     3181 gaacgattcc aacatctttc aagcaaagca agccaaattg tgccgcaggt tgagctgatt
     3241 gaaaatgagg tggatactat taaattggaa atggtatcga gagaattatt ggaagaatag
     3301 tcaaatctct ttattcccta caaaaatatg gggtgatttg aaatactaaa gaaaataata
     3361 aaatgctaaa aaaactatta atcatcgaca accacgactc ttttaccttc aacttggtgg
     3421 atttactccg taagcttgat gtgccggcga aagtggtgct ggttgaagag cttgacctca
     3481 atgaggtgga gcattttagc catattttga tttcgcccgg ccctgatgtg ccgagagcct
     3541 atctacaaac ctttgcgatg ttggagcgtt atcatcaaac caaatcaatt ctaggcgttt
     3601 gcttggggca tcaaacgatt tgcgagtttt ttggcggcaa gttgtataac ctaaacgatg
     3661 ttagacacgg gcagcaacgc acgctcactc aaattgagcc taaccccata tttaaaggtt
     3721 tgcctaagca gttgaaagtt gggttgtatc actcttgggg cattttgcaa aattcgttgc
     3781 aaaatacacc gcttgtacca accgcagttt gcgatcaaaa tgtgttaatg gcgtttaaac
     3841 accaacattt gccgattttt ggcgtgcagt ttcacccgga atcttttatc actgaatttg
     3901 gtcaacaaat gttggcaaat tggcttgaga ttacacaaga tttttagcgt gaaatccgct
     3961 ataatccgtc agttatttac ccttttaaaa tctgtagggg cgaaaaattt ttcgccttac
     4021 ttaatatgca agagataatt taaatgaaag attcgattat caataaatta gaaagtttaa
     4081 gtgaacgcca cgaagaacta caggcgttac ttggcgatgc ctcaattatt tccgatcaag
     4141 acaaattccg tgcttattcc aaagaatatt cgcaattaga agaagtggtt ggcaccttta
     4201 accgctggaa aaagctgaac aatgatattg aagaggctca attattactc gatgatcctg
     4261 atatgaagga tatggcagcg gaagaaattg cggaaaataa agccgaaatc gagaatcttg
     4321 agcaacattt acaaatttta ttattgccga aagatccgaa tgatgaatac aacgccttcc
     4381 ttgaaatccg tgccggaacg ggcggtgacg aggctggtat ttttgccggt gatttatacc
     4441 gtatgtacag ccgttattgc gaaagtaaac gctggcgaat tgaagaaatg tcggcaaatg
     4501 agagtgaaca aggcggatat aaagagatca tcgttaaaat ttcaggcgaa ggcgtttacg
     4561 gtcagcttaa atttgaatcg ggcggacacc gtgtacaacg tgtaccgaaa accgaatcac
     4621 aagggcgtat tcacacctct gcttgcacgg tagcggtaat gccggagttg ccggaaagcg
     4681 agatgccgga aatcaatcca tctgatctgc gaattgacac ctaccgctca tcgggtgcag
     4741 gcggtcagca cgttaatacc accgactctg cagtgcggat tactcatatc ccgaccggga
     4801 ttgtggtcga gtgtcaagat gagcgttcac agcacaaaaa taaagccaaa gcgctggcag
     4861 tgttagcctc tcggattgta caagttgagc aagaacgcca agccgcagag caagcggata
     4921 cccgccgtaa cttacttggc tcaggcgacc gttcggataa aatccgcacc tacaactacc
     4981 cgcaagggcg tgtgactgat caccgtatta acctcacggt ttatcgttta gacgaagtga
     5041 tgaacggcaa aattgatgaa ttgatacaac cgattatcac tgaatatcaa gcggatcagt
     5101 tagcggcgtt gtcggagcaa aattaatttt attgtaaggg tacactgtgt gtgcctgcca
     5161 aatataaatt tgcaaaattt tgactatttt tgatcgcttg tagtttctaa aaagtttaaa
     5221 aatttgagga aaacaaaatg agaaagttag gtttattagc agtatttact ttagtattat
     5281 cagcctgttc aaattcaaca aaaattgaac aaaccattct ttccaagcag gatatcgtag
     5341 gctcttgggt atgcgctatt aaatacgatg atctaaaagt aagcacttta gatttttctg
     5401 aatttaaagc taatggcgaa atgacgaatt tcggtgatat ttctgatgag aattttgagc
     5461 ccattaaatt tacttatgta acaggagata aagggaagtg ggacttaaaa ggaaatcgat
     5521 tagtgattga ttatgatctt tctgaacgta aagtaaaaag aacaacacca aaaaaatttt
     5581 tagagttctt gaaacaggaa gagcgtaaag cctcaaaaac agctaaaaaa ctccttaaat
     5641 atgaacaagg gattttcaat attctgagtg atcagaaaaa tactgaaaac agtaaaataa
     5701 tattagatat tttgaagttt ggtcattcaa gaatggcgat tcagcaaaat atgggagaca
     5761 aagtttattc aggtggttgt gtaacagctg ataaagcaga agagtattta gatatcctaa
     5821 aaaatagaaa ttctaaataa tggattaccg agagtggctc acttttgctg agcaaaaatt
     5881 acttgaaaat agcggggcag atccatacct aaacgcaaaa tttgatgcaa atttgctatt
     5941 gcaagcagtc acaaaacgtt caaaatcggc catttttgct ttttcggaaa ccctactttc
     6001 tgaaagcgag cttgcagaat tagcggaaaa attagctcgg cgtgccaaag gtgaaccaat
     6061 ggcatatatt ttaggctatc gggaattttg gtcgttgccg ttaaaggttt caccggcaac
     6121 gctgattcct cgccctgata cggaacgact ggttgagttg gcgttggaat atgctaacaa
     6181 gcggttagga aatcagaaaa tgttgcaaat tctggatctc ggtaccggca cgggggcgat
     6241 tgcgttggca ctggcgagtg aattaggcga gaaagcccaa attatcgggg tggatttcca
     6301 aaatgaggcg gtgcaactgg cagaagaaaa tcgccgaaac ttaggcttaa atcaagttac
     6361 atttttacaa agcgattggt tcagttcgct tgcaaatcaa caatttgatt taattgtctc
     6421 aaatccgcct tatattgatg aatcggacga aaatttgacg gtgggagacg tacgttttga
     6481 acctttaacc gcattagttg ccgataataa ggggttgagc gatttgcaaa aaatcatcga
     6541 aaatgcaccg cttcacttaa aaccgcaagg ggcgttaatg ctggaacacg gctggaaaca
     6601 aggtaacttg gttcgagatt tatttgattt acacttgtgg gaacaggtag aaactgctca
     6661 agattatgcc ggcaatgacc gagtaaccaa agcgatttta aaataaggca gaaaatggac
     6721 gatattattg aagaattgtt aaaagagttg gaagatccga aagtaaccat cagcgaaacc
     6781 gagctgaaaa agtttcttta tcgtgaaatt ttacgattaa ctacgctcat tgataagaat
     6841 gtaagtgagc aacaagtgtt tggtcagatg tctgcgttgg tgaaaaaagc tcgctataac
     6901 gtgaatggcg aaggcgatga agagagaatt aaccaattac ttcatttagt ctatcgtgaa
     6961 tggggctttc attgctacta tgaagactat ttccacaccg aaaatttgtt gctaaatcaa
     7021 gtaattcgca aacggcgtgg aatgccggtt tcacttgggg cggtggcgct ttatttatcc
     7081 gccgtattgg atttgccgct ttacccggtt aattttccga ctcagcttgt gttacgggct
     7141 gaaatacacc agccgaacgg ggcaaccaaa gtgcgtttta tcaacccgtg gaacggtgaa
     7201 tttctcacca tcgaaatgct gaataaatgg ctggaaggag aaatgggctt tggcgtagaa
     7261 gttacccctg atttgttaag acgagcagaa ccacaagagt tgctggaaag ggtcgaaact
     7321 gtgtttaaaa tggcacttac ccgtgaaggc aaatatgaag aaaccttacg cttgattgaa
     7381 taccgcttgg catttagccc ggaagatccg tacgaaatca gagaccgagg aatggtactg
     7441 gcaagtatgg attgctacca agcagcatta gaagatatta attatttcat tgaccaatgc
     7501 cctgaagatc cttctgccga aattttaaaa atggaagtga aaggactgca acaaaaaagc
     7561 caagaaagtg tggtgcatta agctgagaag tagccccaaa ggggctgagc tatgattaac
     7621 ctagtacggc tctgccgtgc taggcagaaa ataagaaaca aggactccct atgacagata
     7681 aaattgtaaa agttggcaat attgaagtgg ctaatcacaa accgtttacg ctttttggtg
     7741 gtatgaatgt gctggaaagc cgtgatatgg cacttagagt atgtgagcaa tatgttgaag
     7801 tcacgcaaaa actaaatgtg ccttatgtgt tcaaagcctc atttgacaaa gcaaaccgtt
     7861 cttcaatcca ctcttaccgt ggaccgggaa tggaagaagg cttaaagatt ttccaagaat
     7921 taaaacaaac ctttggcgtg aacatcatta ccgatgttca cgaaatttac caatgtaaac
     7981 ctgtggcaga agtagttgat gtgattcaac taccagcatt tttggctcgc caaaccgatt
     8041 tagtggaagc aatggctcgt accggtgcgg ttattaacgt taaaaagccg caatttttaa
     8101 gcccgggtca aatggggaat atcgtagaaa agattgctga atgcggcaac gatcaagtga
     8161 ttttgtgcga ccgtggcact aactttggct acgataatct agttgtggat atgcttggtt
     8221 tcggcattat gaaaaaagtg tcgaaaggtt gcccggtgat ttttgatgtg actcactcat
     8281 tacaatgccg tgatccattc ggtgcggctt ccggtggtcg cagagatcaa gtaaccgaac
     8341 tggcacgctc cggtatggcg attggtttag ccggcttgtt cttagaagct cacccggatc
     8401 caaactcggc gaaatgtgat ggtccatctg cattaccgct gtcaaaatta gaagcctttg
     8461 tttcacagat gaaagcgatt gatgagttgg ttaagagctt tgaagaaatt gatacttcca
     8521 aataaacttt ctccgcatta aatcctttcc tactttcttt gcttcgccaa agaaagtagg
     8581 caaagaaagg cgaccctact tcgctacttt tcttcgctta gtgtgaatgg gcttaacgaa
     8641 aaatttacat tcgcttcgct cagagtaaat tttcctaccc attaaaacac gctcaggtag
     8701 ctcagaaggg agttagaaag gtaaaagaca gaataggaga aaaaaatgct aaatattgtt
     8761 tttcttgacc gtaccggtat tcctgccact cacgatattc ctcgtccgaa ttttccacat
     8821 acttggacgg aatatgccac cacctctgcc gatcaaactt ttgagcgggt aaaagaagcg
     8881 gatattatca ttaccagcaa agtgttactc cagcgtgact tgctggctaa attaccaaag
     8941 ctgaaattaa ttgcgatcac agcaaccggc acgaacaatg tggacttagc ggcagcaaaa
     9001 gagttaggga ttgcagtgaa aaatgtaacc ggctattcca gcgttaccgt gccggaacac
     9061 gtcattggta tgatttactc cctgaaacat cgtttagccg attatcaccg tgatttaatt
     9121 acgtctgacc gctgggcaac ttgcggccaa ttttgctatg tagattaccc gatccacgat
     9181 gtgcgaggct caaccttagg cattttcggt aaagggaaca tcggggctga agtggcacgg
     9241 ctggcacaag cggtcggaat gcaggttatt tttgcagaac atcaaggcgc aactgaaatt
     9301 cgagaaggct acaccgcctt tgaagaggta ttcaaacaag cagatattat ttcactacac
     9361 tgccctttaa ccgagcagac tcaaaacctg attaatgcag aaaccttagc cttaatgaag
     9421 ccgaccgctt atattatcaa tacgggtcga ggcccactga ttgatgaaca ggcattgctt
     9481 gaggcattag agcaaggcaa gattgccggc gctgcgttag atgttttagt gaaagagccg
     9541 cctgaaaaag acaacccttt aatgttagcc gcaaaacgtt taccgaattt actgattacc
     9601 ccacacgttg cgtgggcaag cgattctgcg gttacaacac tagtgaataa agtgacacaa
     9661 aatatggaag agtttgtagc aacgggtatg tagcttgaag taggggctgg tcctgtgcca
     9721 gccctaatat taatagaatg agagggcggg tataaaaccc gcccctacca atatcatttt
     9781 agaaaaacag atgaatttaa cccctttttt tattgctttt cgctatttta gatcgaaaag
     9841 tgctgaccgt ttcgggcggt tagtcaccaa tcttgccagc cttggcattg tgctgggcgt
     9901 aatggcattg gtgattgtgc tgtcggttat gaacggtttg gaaaatatgc agaaaaccaa
     9961 tctgctatcc accttgccgc acgctatcat cacccctcaa gaggggcatt ttgccaaagg
    10021 tgaaaaactg gctctacctg aatttgccaa gcaaagtgtg gtgattaacc gtgcgaatgt
    10081 gattatccaa agttcacaag gcattaacgc cggtcagtta atcggcgtgg aaaatgccac
    10141 agacgatccg ttgttagtgg cggaaaatat ccccgcatta ttgcctagcg gtgaatttaa
    10201 ggtagtaatt ggctctcgtt tagccaataa attgaacgtt acggtaggcg ataagctccg
    10261 cttaatgatc actgaaaaca gccaatatac gccacttggg cgagtgccgg tacagcgttt
    10321 gtttgaaatt tcgggcgttt atcaatctaa tcgtgaagcg agcgaattta ccctctttgc
    10381 taatttagct gatgtcgggc gtttgcttcg catcagcgaa gatcaagtac aaggcgtgcg
    10441 tttatatttg caagatcctt tccaagttac cgaacttagc caatatttcg atgcagaacg
    10501 ctaccaaatc agtgattggc gtgagcaaaa aggcgaattt ttccaagcgg tgaaaatgga
    10561 aaaaaatatg atgggcttac tcatcagcct gattattgtg gttgcaattt ccaacattgt
    10621 gacttcactc agtttgatgg tggtagacaa acagggcgaa attgcaattt tacaaaccca
    10681 aggcttaacc aaaaaacaag tgatgcagat ttttgtgttc caaggggcga ttgtcggagt
    10741 aattggctcg attattggtg gtttgctcgg cgtattgatc gcctacaatt tagatcagat
    10801 tatcctgcta ttaaacccga gcatccatct gcctacccta atttcaggca cgcaaattgc
    10861 ggtcattgta ggggtgtcga tgttgctttc actcctttgc acgctttacc cggcataccg
    10921 agcctcaaaa attgagccgg cacaggcatt gagatatgaa taataagaac ggaaataaaa
    10981 tgacagaatt acttcgctgt gaacatatca gcaaatttta cgatgagggc gaacagaaag
    11041 ttcaggtgct aaaagaggtg tcattttcga tgaacgaagg tgagctggta gcgattgtcg
    11101 gtagttcagg ttccggcaaa agcaccctat tgcacacatt aggcgggcta gatcagccaa
    11161 gcaccggtga agtctggatt cgagggcaat ctctgcaaca gctcagttca gacaaattag
    11221 cgttattgcg taaccaaaac ttaggctttg tgtaccaatt tcatcactta atggcagact
    11281 ttaccgccct tgaaaatgtg atgatgccga tgctgatcgg caaacagaac aaaactgaag
    11341 ctgccaatcg tgccgagaaa atgctacaag cggtcggatt agctcatcga atgacacatc
    11401 gcccatcagc actttcaggc ggcgaacgcc aacgggtggc gattgcccga gcgttggtta
    11461 ataatccggc tctggtactt gccgatgaac cgaccggtaa tttagaccaa aaaaccactg
    11521 aaagcatttt tgagctgatt caacagctca accaagagca gaacattgcg tttttattag
    11581 taacgcatga tttaaatcta gcgaataaac tttcacgtag tcttgtaatg cgagatggcg
    11641 tgttaagcga ggggagctta tgagtacgcc gtttttcatt agctggcgtt accaacgggg
    11701 caaacagaaa aacaaactgg tttcactgat tgctctcttt tccaacatcg gtattgccct
    11761 tggggttgcg gtattaatta tcgggctaag tgcaatgaac ggctttgagc gagagctgaa
    11821 taatcgtgtg ctttcggttg tgccacacgc tgaactggtt tcgtttcaag gcaatcagcc
    11881 tcaaccaatt tttgatagca aaaaattaga ggcattgata aaaaaatcgc aaaatgtgac
    11941 cgctagttcg ccttttgtca gctttaccgc cttaattgaa aatggggctc agcttaaaat
    12001 cgcccaggtg cgaggggttg atcctaaaaa gcaagatcag gccagccaat taagccaatt
    12061 tattccaccc gagcaatggc aagcgttcaa tcagcaattt caagccgatg aagatggctt
    12121 gattttagga gccggtattg ccaaagcgtt agaagttgag gcaggagacg aagttacgct
    12181 tttaattcct cagccaacag aagacggcaa attagcccag cccttgcgtt ttaacctaaa
    12241 cgtaaccggc attttgcggc tagatgggca gctggatcac agttatgcct taattccact
    12301 gaataaagct caagagctga tggaatacca agcgaatgaa gtgtcagggc ttgaaatcag
    12361 ccttgccaat ccgtttgatg tacaacagct tgaaatgcca caacttaacg gctataccca
    12421 accgctttat ttgaatacgt ggattgataa attcggttat atgtataacg atattcaact
    12481 gattcgcacc gtgatgtaca ttgcaatggt tctcgtgatt ggcgttgcct gttttaatat
    12541 tatttccacc ttgattatgg cagtgaaaga caaacaaggc gatatcgcga ttttacggac
    12601 actgggggca aataacggtt ttatccgccg cattttctta tggtatggct taatttcagg
    12661 tatgaaaggg gcgttattcg ggctgatttt aggtgtaatt ttatcgctaa atttaaccgc
    12721 tattatcaaa gcgattgaag cattctttga ggtgaaattg ctttcagacg gggtttattt
    12781 tgttgatttc ttaccaagtg aattacattg gcaagatgtc gtctatgtat tggcagccac
    12841 gattatccta agcctttttg ccagcttata ccctgccaac cgagccgcta aattagaacc
    12901 ggcaaaagtg ttaagtgggc attaatcatt ttcctattct aataaaaaag cagctacccc
    12961 tcgattatgt cagaaaggta gctgctttta tttatgttat tactcttcgc ctaataattt
    13021 caactcacga gccttaattt ggttttttag aatttcaact aagtctgaaa cgaggtaagt
    13081 acctaagtct gcacctttac gggtacgcac agacacttta ccttcggcga tttctttatc
    13141 accgcaaacc aacatataag gcacacggcg taaggtgtgc tcacgcactt tgaagccgac
    13201 tttttcgttg cgtaagtctg ctttcacacg aatgcctgca tcagaaagct gtttcaccac
    13261 ttgttgcaca taatctgctt ggctgtcggt aatgttcatt accactgctt gggttggggc
    13321 aagccaagtt gggaagaaac cggcatattc ttcggtgata atcccgatga aacgttcgat
    13381 cgagcctaaa atcgcacggt gaatcattac cggtgtgcgg cgaccattat cttctgctac
    13441 ataagaggct gctaaacgac ccggtagagc gaagtcaagt tgcactgtgc cacattgcca
    13501 ttcacgtcct aagcaatcac gcaatgcaaa ctcaattttc ggcccgtaga atgcaccttc
    13561 gccttcttga atttcatatt caagtccgtt gttgcgtaag gcgttggcta agcctttttc
    13621 tgctctgtcc cacattgcat catcaccgat acggttttcc ggacgggttg aaagttttac
    13681 tgcaatgtta gtaaaaccga aggtgctgta aatgtcgtac accattttga tacagctgat
    13741 tacttctgac tcgatttgtt cttcagtaca gaaaatatgg gcatcatctt gggtaaaacc
    13801 acgcacacgc attaagccgt gtaatgaacc ggacggctcg ttacggtgac acgaaccaaa
    13861 ttccgccata cggatcggta aatcacggta agatttcaag ccttgattaa agatttgaac
    13921 gtgacccggg cagttcatcg gcttaattgc atattcacgg ttttccgatt gggtagtgaa
    13981 cattaaatct gcgtagtttt gccagtgtcc ggttttttcc caaagcacac gatccatcat
    14041 aaacggacct tttacttctt ggtaatcgta ctctttcaat ttggtacgca caaaagtttc
    14101 aagttcacgg aaaatcgtcc agccgtcatt gtgccagaac accatacccg gtgcttcttc
    14161 ttgcatatgg tataaatcta acgctttacc gatacggcgg tggtcacgtt ttgccgcttc
    14221 ttccaaacgt tgtaaatatt cggctaattg ttttttatca gcccaagctg taccgtaaat
    14281 acgttgtagc attttatttt tgctgtcgcc acgccagtaa gcacccgcta ctttttgtaa
    14341 tttgaagtgg tggcagaaac gcatatttgg tacgtgtgga ccacggcaca tatcaatata
    14401 ttcttcgtgg tgatataacg caggcgttgc cgtacgttca atattttcat ctaaaatcgc
    14461 cattttgtat ggctcaccac gtttctcaaa ggtatctcga gcttcttgcc agcttaccgg
    14521 gcgttttacc acgtcatagt tggtttttgc cagctctaac atacgctttt caagagcgtc
    14581 taaatcttct tgagtcaaag agcggtctaa atcgatatcg tagtagaaac cgttttcgat
    14641 agtcgggccg atagccattt taacgtctgg gaataattgc ttgatggagt gacctaacaa
    14701 gtgagcggtt gagtggcgaa tgatctctaa accatcttcg tctttggcag taatgatttc
    14761 aagtgtgctg tcttggctga tgatatcaga cgcatcacgg cgttcaccgt ttacacgacc
    14821 ggcaatggtg gctttcgcta aacctgcccc gatagattgg gcaacttcca ttacagaaac
    14881 tgggttgtca aatgaacgtt gtgaaccgtc cggtaaagta ataattggca taataaatcc
    14941 ttacagtggt aatccatacg agagattact tgtgttgttt gaatgaaaaa accgcctaca
    15001 agcggtcttt tttgattaaa attttgcaat ttgcttatta tttcccttac aagtggggaa
    15061 taaaagcact gaaattctag cataaaacaa agaaaaatta aactttgctg ttttcagcgg
    15121 ccccttcatc aagctcaata ttttcatttt ctgtttgtgg atcttgttca agtatttcaa
    15181 gctgagcctg gtactcttgt accgcttctt catcaaatgg aataagtgcg tcctcaacaa
    15241 cttcaatttc ttcaagtggg cttgcggttg caattggggt agcatattgc atctctgctc
    15301 gttccgcttt cggaattgat ttggtgcgga aagcacaaat aaataaacta agtgagaaca
    15361 gtccaaaaag tgcataaaag ccatattcag cggaaaggct catcgcaaaa ccgataagcg
    15421 gtggagcaac taacgccccc acaccatagc aaaacagtaa acccctgctt acttcaaccg
    15481 tgttcatatt attcggcaat tcatcgttgg cacgggcaat gctcaaagca tataagctaa
    15541 acagactaca gccaaagaaa aaggcgataa tatactgtcc ccaggcggtc aaattttctt
    15601 ggaaaattgc aaataatccg gcaagtgcgg ttaaagtggc aatcactgaa caagctaaaa
    15661 tggcgttacg tctgccaata cgattcgata atatcgccat cggcatttgg ctgataaaac
    15721 caccgatcat cgagatcatc aaataaagcg agatttcact taatgaaaag ctaaacttca
    15781 gcagaaagac tgacgccatt gtaaaaaagc cgtttaccaa cattccgcta ataaagctgg
    15841 ttgctaaggc taacggtgaa atcgcaaata gtttaggaat actaatgcgc tctcgagccg
    15901 gaatatcagg ctgaaccatt ttagttaaag caattgggat catcgacatc acaaccaaaa
    15961 tggtagataa gataaaaatg tttcggctac taagttctaa acttaataaa cttacgccta
    16021 aaataaaggc gagatagaag ataatgttat aagtggcaag cactttgcct ctggtatcga
    16081 cattggtttt ttcgcttaac caactttcga ctaaaagcag caagctataa tgacagaatc
    16141 ccaaaataat tcgtaacacg ccccagatcc acaaagtatc aatcataata tgaccgagag
    16201 cggcgatagc aaaaacagca cagaaaacgc taaatgaacg gatatgccca acacttgata
    16261 caatgcggtg agcaaaaata gtgcttaatg ccgccccaat aaagaaagca gcgttaagta
    16321 aaccgatgat gatgtccgtt atccccattt ctgccagttt gatccctgct gaatttagaa
    16381 ataaaccgta gccgaaaaac agaaaaaaga tacctgaata aagggagtaa aatttagtgc
    16441 tattcatcaa tgctccttca aacattctgc cagtattaaa tcattgtacc gatttaatac
    16501 cttttaggca aaaaagttat tattagcgtt gagaaaataa cttttggcta ttttgattaa
    16561 aaaatcaaat gaaggtgatt ttgaaatgta gtaaaaaata gagcaatcag aattgaaaaa
    16621 gggtttgccg taacaaagcc tttagaaaga ttagagtttc gagaagaatt tgtagcttgg
    16681 gctaaccaaa acacttgcta ggagtgcttt aagcacacca cctaaaataa atggatataa
    16741 gcccgcttgt aatactgtgc cttctggtac aaagaaagtt gagccaagcc gaaagcaaag
    16801 gtgatcacac ttgcaattaa ggcgacaaat aaacttttcc aaaaagagtt taatacgcct
    16861 ttatcgctat aatagcctaa gaaataggcg gttggaatat agcctaataa gtatccgcct
    16921 gttggaccaa agaaatgatg gaaacctgaa gcaccgccgg caaagacagg taaaccaatc
    16981 gccccttcaa aaagataaag taatactgca gcagttgcgg ctttacgacc cattgcaaaa
    17041 ccaaccacag ttaaagcaag tgtttgccct gtaatgggta cagggtacat cggcacagaa
    17101 atttgtgcaa aaatagcaag cagtgttgct ccaataaaag ttttcaacca aaatgtagtt
    17161 atactattgg cttctagcca acgtttagtt tgaattaatt gcgtagtgaa cataatcctc
    17221 tcctttaata tggttaaaat gttctcaacc gattatataa gaagatgtct tttagggtaa
    17281 gagtgaattt aaaatggtaa aaccagttta tatttattgt gatgaaggta gcagtgaaat
    17341 tggtgttcaa tcactgttat tggcagtaaa acagcattta cagcgtccgg cttatctcat
    17401 caatgccaaa caaattattc aaagcaagtt ttccgatggc tgtttattaa tcatgcccgg
    17461 tggagccgat ttaccttact gcaaaaaatt aaacggagaa ggcaatcgta gaatccgcca
    17521 atttattgaa caaggtggag cttatctcgg tatctgtgcc ggagcttatt atggctgtgc
    17581 tgaattgaat tttgtagggc gtgactatag cgtaaatggt aatcgagaac tagctttatt
    17641 taatggcatt gcgggcattg cgaaaggcag tctgccgcag tttaccaacg ggcaacttta
    17701 cgataaaaac attggtagca aagcgatagt tgaagtaaca cttgcagagg gtggaaaaat
    17761 gccgttttat tatcacggtg gttgctgttt tgaaccaaat tcaacaacag tgatttacaa
    17821 cccgttggct tattatcctg atggctcagt agcaatgata gagggagagc tagggcgagg
    17881 ttactatctg ttgtcgggtg tacattttga attacaagcg gtcgtttatt ggcaaaaaat
    17941 tgtaaattgc tgtgaaaatc ttgacataaa aacacaagaa gaagcaattt gctctaattt
    18001 taatgtaaat tatggctcgc aaatttggca acatctaaag caacgactaa atcaagcgta
    18061 aaaaaataac ctttcgctat tctgccaaat ggtttctgca atcagatttg gtgattctgt
    18121 tcttaattca catagactat tgaaagtcac taaaagccgt tccggtcggt tcggctggcc
    18181 ttgaaagcca aacaccggca tatcgggcga gtcggtttcc agtagtaaac tttcaagagg
    18241 tagtttgcgg atagcctcac gggttttgtt agcacgttca taggtaatag ttccccccac
    18301 accaatttta taaccgagat cgacaaaacg ttttgcctgt tcatagctgc cggcaaagcc
    18361 gtgaaccaca ccggttttgg ttagttgggc ttttttgagg aacacaaaaa gttgatcgtg
    18421 gcttttacgt gaatgaagat taacgggtaa attaaatttt ttcgcaaggg aaagttgtgc
    18481 ctctaaaaat tcacattgct ttttccataa ttcaggggta gaaacttcac ttaccgcttt
    18541 ttccaaaccg atttcggcaa tagcggtaca ctgtggatct tgttggctta gcaaattttc
    18601 caataagatt aaatcttgtt gttggtgctg ttgaatatac agtggatgca accctaatcc
    18661 atacaccaaa ttttccggtt tttgtttggc acaagcggtc acttttgcaa aattttttgc
    18721 aaatacggca acgataagaa tgcattcaac ctgatgctgt ttggcattct caaccaattc
    18781 cggaatgctt aaattcaggc tttctgcgag atagtcgagg tgagtatgag tgtcaaaaaa
    18841 tcgcataata aaaccaataa aaacggggac tagattgtcc ccatttttag cattttacgc
    18901 aagcaattag tttgttacat aagcatcttc atcacgagcc tcagatggct tgaggaaagc
    18961 aaagaagcaa ataacacaaa ctaccgtaat cgcaaaaccg atataaactg ataattcata
    19021 atctaaaccg aagccgattt tgttgttcgc aaggtaagtt gcacacacgg tagtgataaa
    19081 ccatgcagga atcgagcata cccagtggaa ttttttatag cggtataagt aacctgcggc
    19141 agtccataac ataaccattg cagtcatttg gtttgcccaa gtgaagtaac gccataatac
    19201 gctgaaatca acttttgaga caatgaaacc aagtacgaat aatggtacgg caatatacaa
    19261 acgttttact aaggtacgtt gttctaaacc aaagatttcc gctagttgta aacgagcggc
    19321 tcggaatgca gtgtcacctg aggtgattgg taatactaca acgcctaata cggcaaaaat
    19381 accaccgata aaccctaaga agtgtaacga gctgtcatat accactttag acggtgaacc
    19441 ggcagagatc gcatcttgta aggcttgtgg gttttcatag aatgaaagac ctactgcaca
    19501 ccatactaat gcaattaccc cttcagtaat cattgcaccg tagaaaataa aacgaccttc
    19561 tttctcattt tgcgcacagc gagccattaa tggggtttgg gttgcgtgga aaccggataa
    19621 tgctccgcaa gaaatagtta agaatagtaa tggccaaatt ggaacatcac ctttcacttg
    19681 gaagttttgg gtgaattttt cccacgttaa gccttgaccg tctgcattca ctgtacggaa
    19741 gaagtcgata ggatcagtcg cacttaagtg agcgctgact agaccatata ccatacctac
    19801 agacataaag agtaataatg ctccgaagaa aggataaatt cgaccgatga ttttatcaac
    19861 aggtagtaat gtagcaagaa tatagtaagc aaaaatgatt aacgtccaca cagaaatcac
    19921 ggttgcttta tccatacccc aaacggttac gccacctgat gctacagatt ctttaattgc
    19981 ttcaacatca cctaattcca ttgcactttg ggttgcacct gcaatatcca tggtgatggt
    20041 agagagtaac tgagcagggc ttgcaacgaa aactacgcct actaataaaa gcaagatgag
    20101 cgccattacg ttgatgaata acttgactgg acgacccaag aatttccctg ccagatttgg
    20161 catcgttgcg ccaccgttgc ggatacttaa cataccacag aagtagtcgt gtactgcacc
    20221 ggcaaaaata caaccaatca caatccaaag cattgcaact ggtccgtata aggcaccaag
    20281 tattggtcca aaaatagggc cggtaccggc aatgtttaat aattgaatca gccaaatctt
    20341 ggttttagac attggcatat agtcaacacc gtcgttcatt gcataagccg gcgttgctct
    20401 tttcgggtta atcacgaaga ttttttcaat gatttttccg taagtaaagt agccaagaac
    20461 aagcacggct acacagaaaa agaaccacaa catagtagaa atcctataaa ttgagataaa
    20521 tgatcatttt tagcagaact gataaaggcg tgccattgta agtgaggagt gaggataaag
    20581 taaaatgaat tgttagattt gtgtgaataa tttactgaaa atgtgaggtt catcaaaaaa
    20641 tccctttgtt ctgcgtcaat caaagggact ttttaaagtt aaatatcgaa tttttgaatt
    20701 ttcgcttcaa tgccttgggc atctaatcca agatcggcat aagcttcagc ttgagtagat
    20761 tggggaacga aaaagtcagg aataccaagc attacaagcg gtttgatttt gccgatattt
    20821 tgcaaatatt cattcacaaa actacctgcg ccaccttgaa tagcattttc ttcaagcgta
    20881 actaacaact cgtgtgaatc cgccactttt tgaagtaatg cttcatctaa tggtttaatg
    20941 aaacgcatat ctaccaacgt gtagttatgt ttttcagcca caatctttgc ttcatcaagc
    21001 aaagcaccaa aatttaaaat cgcaactttt ttaccttcac gaataatgtt tcctttacct
    21061 acttcaagtc tttgcattgg ctcaagtttt acccctttag cattgcctcg agggtaacga
    21121 atagcacttg gcgtattcat cgtgtaagcg gtataaagca tttttcgcat ttcattttca
    21181 tcgctcggtg ccataatggt catattggga atacaacgca taaaacttaa atcaaatgca
    21241 ccttggtgag tttgcccgtc agcgccgaca attccggctc ggtcaatcgc aaaaatcacc
    21301 ggcaggtttt gaatagcaac atcgtgaatt aattggtcat aagctcgctg caagaagctg
    21361 gaataaattg cgactaccgg tttgtaacct gcgattgcta agcctgcccc gaaagtcact
    21421 gcgtgttgtt cggcaatcgc cacatcaaaa tattgttcag ggaaacgttt ggaaaattct
    21481 accattcccg agccttcacg cattgccggc gtaatgccga taattttcgg atcacgctct
    21541 gccatttcac atagccaatc gccaaaaata ttggaataag taggtgctgc ttttgatttt
    21601 ggtaactcgg ctgcggtagg gtcaaatttc ggaacgccat ggtagccaat cggatcattt
    21661 tccgcaggtg tgtagccttt gccttttttc gtgcgaatat gcagaaattg agggcctttc
    21721 agctcacgca tattgctcaa ggttttgact aactcgtcaa tattatgtcc atcaatcggg
    21781 ccgatataat taaagcctaa ctcttcaaac aaagtgcttt caggggagaa aatcacccct
    21841 ttcatatgct cttcgctttt tttcataaag tttttgatgg taggcacttt atctaaaatt
    21901 ttcttactgc cgtcacgcac tgaagtatag agcgaaccag aaaaaatgcg ggcaaggtgg
    21961 ttgtttaatg ccccgacatt ttcagagatt gacatttcat tatcatttaa gataaccaac
    22021 atatcggtat gcaatgcacc tgcgtggttt aatgcttcaa atgccattcc ggcagtgatt
    22081 gcaccatcac cgattacaca gacggtttta cggccggcat tttctttttc tgctgccacc
    22141 gcaatgccaa gtccggcact gatcgaagta gaagaatgcc caacgcttaa cacatcgtaa
    22201 gggctttctt cacgccaagg gaaggggtga ataccatctt tttggcggat agtgtgcatt
    22261 tgatctcggc gcccagtaag gattttatgt gggtaagctt gatgaccaac gtcccaaatt
    22321 aattggtcga aaggcgtttg ataaacataa tgcaaagcta ccgtaagctc tactacccct
    22381 aagccagagg ctaaatgtcc gctagtttga ctgactgatt ccaataaata ggctcgcagt
    22441 tcatcaacta cggtttgaag ctggtcttta gaaagtaaac gtaaatcttc cggtgagttg
    22501 atttcgctta acagaggata cttcttttcc ataatgtttt attacccaca accatcaaat
    22561 aatagatgat attaaattaa gctttgagtt cttcggtcaa agtaggctga atgctattta
    22621 aaatttcttt aaccacacgg ccgttaccaa caaccagatt acctgttttc aagtaatctg
    22681 ttccgccaac aaagttagtc acaattgcac cggcttcacg ggcaataagt tcaccggcgg
    22741 cgaaatccca cggttttaag ccgatttcaa agaaaccatc aacacgaccc gctgcaacat
    22801 aggcaagatc taaagcggca gaaccggtac ggcggaaatc agctactccg ttattcatta
    22861 agccttcaag catatttaaa tgtgcatcac gatgtctgat gactttgaaa ggaaaaccgg
    22921 ttgcaattac agtgcctttt aaatcacggc gttctgcatc aatgcgtaaa cggaactcgt
    22981 ttaatttagc cccttcgcca cgcaccgcag tgaaaagctc gttacggata ggatcgtaaa
    23041 ctacccctac tgttgtacgt ccgttttcac gcacggcaat cgacacagca aaatgaggta
    23101 agcgttttac gaaattggtt gtaccatcta atggatcgat cacccattgt acattttcat
    23161 tgctaccgtt taacacgccg gactcttcgg tcacgatcga gtgttcaggg taagatttac
    23221 ggatcacatc aataattgcc gcttccgctg ctttatcgat ttcagtcaca taatcattcg
    23281 tatttttttg taaaacttgt gtatcttgag gcgattgttc atagcctttg gcaataatat
    23341 tgcctgcttt tcgtgccgca cgaatagcaa tgtttaacat tgggttcata tttccaccag
    23401 attttaaaga acataataaa gcacgaatta taaagaatgt agcgaaataa agaaacaaca
    23461 agcggtctaa tctgtgaaaa attttgcaaa aaaatggctt tttctaaccg cttggagttt
    23521 ggattcgtta taatacctcc cattcaagcc acccatttag gtactaaaaa tgcaatttaa
    23581 agaaaataac caacctcgtt ttaaacacag agaagaacgc cgttcagatg agtgttcccc
    23641 ccgtttttca gataaaggca aaatgcgagg aaatgaaggt aaaagtgaca gaccgcataa
    23701 gctacatttt aagcgtgacg aggcttattc acgctctgag cagatttatc cagcagaagg
    23761 caaaaaagcc agtggtgaag gtagtgtgca aatttgggta aaaggtggga atagctcagt
    23821 taaagagaaa aaaaacggcc cgctttcacc gagagcgcct gagaaaatta agaaaaatcg
    23881 cttggaagag atgaaagtct atggcgaaaa tgcctgtatc agtttgttcc aacaacgtcc
    23941 tgaatctatt gtacgtttat gggcaacagt ggagggggca aaaaaattag gcgatttaat
    24001 gagctatttg gctcagcaca aaaaagccta tcacgttatt gaccgtgaag agatggagcg
    24061 agtcaccggt actgaacatc acggcgatat gtgcttattg gtgaaaaaaa cggcttcatt
    24121 tgtgctagag ggctatttac aagtgccgaa aaaacaagat tgcttggtac tgttagatgg
    24181 tgtaaacaat gctcagaatg tggggggaat catcagaacg tgtgcttttt atggtgtgaa
    24241 aggcattatt actgaaaatg tggatagctt aaactccagt gctgcagctc gtgtcgcaga
    24301 gggtggtttg gaatttgtgc gagcgttaga aactaaaaac aagcatattg ctttggtaca
    24361 actccgccaa gccggttatc aaattgtcca cctcacacgc cataagcaag ccccttcttt
    24421 aaccaaaatt aagctggcgg aaaaggtggt ttttgtatta agtgagattc ctgcaagtga
    24481 tgtggcttac gcagaagaca caacaataca gctttccttt aataatccgt taaatagtgg
    24541 tttaaatgtg gcggtaaacg caggtattct acttaatcaa tggtatcaaa cccacattct
    24601 ttaaggaatt gaaatgaatc agcaaaaacc gtttgaagtg attgcgattg ttggcacacc
    24661 tcgccacgac agtgcatttg aaacacatct tgccgtttat aattggttaa aagatcgtga
    24721 ttatcaaatt ttagtggaaa cagaggttgc cactcagtta cagcttaagg aaagctacag
    24781 tttagatcag attgggcagc gcgcagagtt ggtgattgtg ataggtggcg atggtaatat
    24841 gcttggcatc gcccgagctt tggctaaata ccgagtgcca ctgataggca ttaaccgtgg
    24901 caatctgggt tttttaaccg atatcgcacc tcaaacggct tttgaacagc tttatagttg
    24961 cttagtcaaa aatgagtatg tgattgaaga gcgattcctg ttagaggctt tcattgagcg
    25021 caacggtaaa attattgccg caaataatgc cttgaatgaa attgtggttc acccaaccca
    25081 agtggctcga attattgaat ttgaagttta tattgacgga aaattcgctt tttcacaacg
    25141 tgcagatggc ttgattgtgg caacgcctac aggctcaacc gcctattcac tttctgctgg
    25201 tggcccgatt ttaaccccga atatgaatgc aatggcatta gtgccgatgc accctcacac
    25261 tctttcttct cgtcctttgg tggttgatgg cgatagtcaa atttcgcttc gttttgccaa
    25321 atataatacc cctgatttag aagtgatttg tgacgggcag gtgaatatgc actttacgcc
    25381 tgaagataga gtcttggtgc gtaaaagccc ggataaatta cagctgctcc atttaaaaga
    25441 ttatagctac tttactgtat taggttctaa attaggctgg gcaagtaagt tgttttaaac
    25501 acaagcggtg ttttttctga ttaaatttgc aaatgcaata tgaattgatt tcatatacat
    25561 ttcaaaaata tatcattttt attcaataaa aatttgtggt tcaataagct atctgaaaaa
    25621 taaaggggac aataaaatga caacaaattt taaatgccaa gtgcatacta ttgaatttaa
    25681 tgaagattac caaccggagc aaagcacccg tttaaccacc aactttgcca accttgcccg
    25741 tggcgaaaac cgcaaacaga acctgcaaaa tgcgattgcg atgatcaata accgttttaa
    25801 cgatttagcg aactgggata acccgaatgc ggatcgttat tcggtgaaat tggatattgt
    25861 ctcactggat attgatgtgg aaggcaaagg cgagtttttc cctgcgattg agtggctgaa
    25921 aacctatgtt atcgactaca aaaccggcaa aaccattgat ggcattatcg gtaataactt
    25981 ctcttctttt gtgcgtgatt acgatttcag cgtattgttg ttagatcaca ataaagacaa
    26041 accgcaattt agcgtgccgg aaggctatgg cgagttacac ggcaagctgt ttaagcattt
    26101 attggcatcg cctgaatata ccgcaaaata taaaaaacca ccggtgatct gtttaagcgt
    26161 ggcaaccacc cgtacctaca caagaaccac taacgttcac ccaattttag gtgtggaata
    26221 tctgcaagat agttattcat taactgatgc ctattttgcg aaaatgggct taaaagtacg
    26281 ttactttatg ccgccaaaat ccgttgcacc ctatgcgttc tactttgatg agaatgcaga
    26341 tttactcaat gattacacta acttagagct aatcagcacg attgccacaa tggaagcgtt
    26401 ccaaaaaatt taccgtccgg aaatttacaa tgccaattcg gtcgcagggc aggtctataa
    26461 aggcagcctg acttaccaag attattccgt caccaaagtg gaatatgacc gtgaagaacg
    26521 cacccagttg gcgatcaaac agggtaaata caccgaagag cattttatta aacgttacca
    26581 agcgatttta gaggcttggg cgacggagga ataaacgtag ggtgggcgta agcccaccat
    26641 aaaatgagat ataaccaatg gtgggcttac gcccacccta ctttttatag gaaaattcaa
    26701 aatgagcaac attcttctcc caacctctac cgccggcagc ctaccaaaac cttcgtggct
    26761 tgccgagccg gaaaaactgt ggtcaccttg gaaatttgaa ggcgaggaac tacttcaagc
    26821 caaacgtgat gccctccttg tttcccttgc cgaacaaatt gcggcaggga ttgatattgt
    26881 tagtgatggc gaacagaccc gccagcactt tgtgaccacc tttatcgagc atttggaagg
    26941 ggtggatttt agcaagcgtg aaatcgtgcg cattcgtaac cgttacgatg ccagcgtgcc
    27001 gagcgtggtg ggggcagttt cccgcccgaa atcggtgttt gtggacgatg ccaaattctt
    27061 gcgtgcgcat accgacaaac caatcaaatg ggcattaccc ggcccgatga caatgatcga
    27121 cactctctat gacggtcatt acaaaagccg tgaaaaattg gcgtgggaat ttgccaaaat
    27181 tctcaacgaa gaagccaaag agttggaagc ggcaggggtg gatattattc aatttgacga
    27241 accggcattt aacgtcttct tcgatgagct gaacgactgg ggcgtggcgg cgttagagcg
    27301 tgcggcggaa ggcttaaaat gcgaaacggc ggtgcatatc tgctacggct acggcattaa
    27361 agccaatacc gactggaaac agaccctcgg caacgaatgg cgtcaatacg aagagagttt
    27421 cccgaaactg caacaatcaa aaatcgacat tatctcgtta gagtgccaaa actcccgtgt
    27481 gccaatggac ttaatcgaat tagtgcgtgg caaaaaagta atgctgggag cgattgatgt
    27541 ggctaccaac attatcgaaa caccggaaca agtggcagat acgttacgca aagcattaca
    27601 atttgtcgat gcggataaac tttatccaag caccaactgc ggaatggcac cactttcacg
    27661 ccaagtcgca caaggtaaat tacaggcact cagccaaggg gcagcgatat tgcgtgcgga
    27721 attcgcaaaa taattttgcg atctgcatcg aaaatatcaa ctttgctttt tgctcgtttc
    27781 cagattttct tccaaaataa caggaaatga cggaggcact atgctaaatc tatttaaaaa
    27841 gccaattaag aaaagctcct ttgaggcttg ggcaaaacta atggacgatg tggcaaaagt
    27901 tgcaatttta gcaattcctg ttattctcta tggagaatat tcactgctaa tgaaatttac
    27961 acactcaatt accttatttg cagtagttta ttttttgtta gcaacggcaa gattgtttcg
    28021 agaaacagca gaggagatgt aaatggacgg attgacaatc ggattatttc ttttgcttgc
    28081 attttcattt gctgcatacc aatgggcaaa gcatttaaaa gatcataatc attaattcaa
    28141 tgaataatac taagcggtca tatttggtaa attttttaca aaatgtgacc gcttgttttt
    28201 ctttcactcg aagttaattt ttatataaat aatactatct atgaaactaa aaaatgcgta
    28261 aaataacgtg aaatcaactt acgcaaacgt ttacttaagc ttattaggag tccaaaaatg
    28321 ttaaaacgtg atatgaatat cgctgattac gatgcagtat tatggcaagc cattcaagat
    28381 gaagatcgcc gtcaagaaga acatattgaa ttaattgcat ctgaaaacta tgcaagccca
    28441 cgtgtaatgc aggctcaagg ctcacaatta accaataaat atgcagaagg ttatccgggt
    28501 aaacgttact atgggggttg tgagtatgtg gatattgtag aacaacttgc gattgaccgt
    28561 gcgaaagcat tgttcggggc agattatgcc aacgttcaac cgcattcagg ctcacaagcg
    28621 aatgcagccg tatatatggc gttgcttaat ccgggtgaca ctattttagg tatgagttta
    28681 gcgcacggcg ggcatttaac ccacggtgct tcagttagct tctctggtaa aatttataaa
    28741 gcagaacaat acggcattac tgatgaaggc ttaattgatt atgaagcatt acgcaaacaa
    28801 gcccaagaag tgaaaccgaa aatgattgtt gctggtttct ctgcttattc tcaagttatg
    28861 gattgggcaa aaatgcgtga gattgccgat gaagttgggg catatttatt tgtggatatg
    28921 gctcacgttg caggcttaat tgcggcggga gtttatccaa atccgcttcc tcacgctcac
    28981 gtcgtgacga caacaaccca taaaacctta gccggccctc gaggtggttt gattttatct
    29041 gcatctggcg atgaagaaat gtataagaaa ctgcaatctg ccgtattccc tgctggtcaa
    29101 ggtggaccat tgatgcacgt tattgcggca aaagcggttt gttttaaaga agctttagag
    29161 ccggaattta aggcatatca gcaacaagtt gtaaaaaatg caaaagcaat ggttgaggta
    29221 tttaagcaac gtggttttga tgtggtttca aacggtacgg aaaaccattt attccttgtt
    29281 agctttgtaa aacaaggttt aaccggcaaa gcggcagatg ctgcattagg agctgcgaac
    29341 attaccgtga ataaaaactc ggtgccgaac gaccctcaaa aaccattcgt gacttcaggt
    29401 attcgtgtgg gtacgccttc tgtgacccgc cgtggtttta aagaagcaga ggtgacagca
    29461 ttagccggct ggatgtgcga tgtgctagat agcattggta aggataatca cgaacaggtg
    29521 attgcagaga ctaaagcgaa agttttagac atttgtaaac gtttaccagt gtacgcataa
//
LOCUS       DANLZC010000011        28611 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.011, whole genome shotgun sequence.
ACCESSION   DANLZC010000011 DANLZC010000000
VERSION     DANLZC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28611
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.011"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            19..300
                     /locus_tag="PXE44_000407"
     CDS             19..300
                     /locus_tag="PXE44_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDL5587953.1"
                     /translation="MTKSELIESLASKNPSLPIKMVEHCVKELLEQLTATLEEGERIE
                     VRGFGSFSLHYRQPRLGRNPKTGESVLLGAKYVPHFKAGKDLKERVDLL"
     gene            337..624
                     /locus_tag="PXE44_000408"
     CDS             337..624
                     /locus_tag="PXE44_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL5587954.1"
                     /translation="MIKYILGILIVIAVIIVAVTIGANNDQLITFNYIFAQSELRLST
                     LVAILFGLGLILGWLVTGIFYLKIKFQNVALNRRVKRQAQQITELTTPKAE"
     gene            628..1824
                     /gene="lapB"
                     /locus_tag="PXE44_000409"
     CDS             628..1824
                     /gene="lapB"
                     /locus_tag="PXE44_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDL5587955.1"
                     /translation="MLELLFLLLPIAALYGWYMGQRSAKKDQETASNKFSREYVTGLN
                     FLLSNQQEKAVDLFLSMLQKQESENHIASESQFEAELTLGNLFRSRGEVDRALRIHQT
                     LVNSPHYSFDQKLLAKQQLAKDFMVAGFYDRAENYYISLVDEPEFAKYSLSQLAVIYQ
                     KTREWKKAINVTEKRLRIEPDADKIPLSHFYCEYAQGVKFDDKAAFIESLNKALEYYP
                     QSTRASIILGDFYLEEGEYLRALSYFEQILVQDPDYISEVLGRIKQSYMALEDLNGYE
                     LFLIRANRVKHNSSVDIALTEFIEEKDGINAAHSKLYQQLSTYPNLITFHRFIRYQAD
                     FAEEGNGKESLVLLHNMVGNQIKRSFQYRCLNCGYQSYRLMWQCPSCNQWEKIKPVQS
                     IEGIIQ"
     gene            1848..2540
                     /gene="pyrF"
                     /locus_tag="PXE44_000410"
     CDS             1848..2540
                     /gene="pyrF"
                     /locus_tag="PXE44_000410"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL5587956.1"
                     /translation="MNNKVIVALDYETEREALEFVDLVDPSLCRLKVGKEMFTTLGTN
                     FVKQLHERKFDVFLDLKYHDIPNTVARAVRSAADLGVWMVDLHASGGLAMMEEAKKIL
                     EPYGKEAPLLIAVTVLTSMEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQE
                     VEVLRTHCGQDFKLVTPGIRPKGTDFGDQRRVMTPKQAVETGSDFLVIGRPITQAQDP
                     LAVLKSINQSIS"
     gene            2623..2934
                     /gene="yciH"
                     /locus_tag="PXE44_000411"
     CDS             2623..2934
                     /gene="yciH"
                     /locus_tag="PXE44_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDL5587957.1"
                     /translation="MTLVYSTETGRIKSEVIKEERPKGDGIVRIQRQTSGRKGKGVCL
                     ITGLDLEDKALNSLASELKRKLGCGGSFKDGVIEIQGDNRGFIKQILEQKGFKVKLAG
                     G"
     gene            3037..5199
                     /locus_tag="PXE44_000412"
     CDS             3037..5199
                     /locus_tag="PXE44_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDL5587958.1"
                     /translation="MWKKTLIAVFLASQYAFAVGTGKEPSKSYTETELKQLKNDWLAI
                     YQKELQYAENLRLKQRENFQQINYLADAAISQKNLSLSTEQIIQRLLLTLAGYPLEMK
                     AEWKLLNAKITLKQATESEVKAFISKYPDSLYQRQLQNLMFELLYREQKFPELLEYAQ
                     NVKQSSLADQCRLFSARYELAASKAQINPEVSQVTEQKAENAELLALVQEFDRFWLAT
                     PKLTSDCANLESYWRDQGLKTDEKMRLKAVELIKQNANAELANLAANVNNENLKIWLS
                     SVEKVANNPADLQNFIENQPLDSPFYADNKAIIQQLFAKYVRTLSENMENPSFQQYQT
                     WAEKYQLSSEEINGWKNAFITRFFDNPEPTFQFWRDEQIKLLKTDNLTERRLRMAIWQ
                     KADLNEWLVLLSQQAKEKAEWRYWAAKEEKNEAKRKVDFESLAKERGFYAMLAAQQLG
                     IVYQIPMLEVPELTQAQLELYANQLARIQELRELGQFDQARKIWIDWIKNIKPEAGEN
                     TPTKIQLALIKYAKDKNWYDLAVEGTIQTKAFDYLDLRLPNAYSDWFDLHLQDKKISK
                     TFAQAIARQESAWNAQAQSHANARGLMQLLPTTAQKTAKDNGLTFKDGNDLFQPFNNI
                     MLGTTHLMELNEKYPNNRILIASAYNAGAGRVEQWLKRSNGQLAMDEFIASIPFYETR
                     GYVQNVLAYDYYYQMLYSDINDKNGLKMFYQEELTRKY"
     gene            5248..5324
                     /locus_tag="PXE44_000413"
     tRNA            5248..5324
                     /locus_tag="PXE44_000413"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5282..5284,aa:Met,seq:cat)
     gene            5551..6012
                     /gene="rimP"
                     /locus_tag="PXE44_000414"
     CDS             5551..6012
                     /gene="rimP"
                     /locus_tag="PXE44_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL5587959.1"
                     /translation="MATLEQKLEDLVLDSIEALGFELVGIECQRAGRFLTVRLYIDKE
                     GGVTIDDCSDVSRQVSAIFDVEDPIADKYNLEVSSPGLDRPLFTVAHYERFIGQDVVI
                     HLRIPMFDRRKWQGKIKQVEGDFITLTVENEDRQFAFGNIQKANLVPVFNF"
     gene            6033..7529
                     /gene="nusA"
                     /locus_tag="PXE44_000415"
     CDS             6033..7529
                     /gene="nusA"
                     /locus_tag="PXE44_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL5587960.1"
                     /translation="MSKEILLAAEAVSNEKQLPKEAIFEALETALTIATKKSRDLDID
                     VRVVVDRKTGDFQTFRRWAVVENVNNMTREISLEAAQFEDPNVQLGDFIEDEVESIEF
                     GRVAIQTFGQVVKTKIREAEKTKIIDEFRSDLGKIILATVKKVTRDNIFLELQGKSED
                     IKGEAVIVREDMIPRENFRPGDRVRGVLYEIKPESKGPQLFVTRAKPVMLEELFKLEV
                     PEIGEGVIEIKGSARDVGSRAKIAVKTHDKRIDPVGACVGMRGARVQAISNELGGERV
                     DIVLWDENPAQFVINAMAPADVSSIVINDETHSMDIAVEAKNLAQAIGRNGQNVRLAT
                     QLTGWALNVMTVEELTKKSEEEDGKVINLLTSALEIDEEFAQVLIEEGFTSLEAIAYV
                     PVDTLTAIDGLEDEDLVEELQSRAKNAITKKALEEEKLLKSAQIEEKLLTLEGMDRHI
                     AFKLAEKQITTLEELAEQGVDDLADIEELSEEKAGELIMAARQICWFN"
     gene            7514..10081
                     /gene="infB"
                     /locus_tag="PXE44_000416"
     CDS             7514..10081
                     /gene="infB"
                     /locus_tag="PXE44_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL5587961.1"
                     /translation="MLVQLSKEDNKMTEEKKTLSLGGARKASKVNTTTTSGKVKAVEV
                     KEKKPKIDAKALQEKAAKEAAAAKARAEKEAAEKLAKEKAEAEAKEKAAKKAAESTAS
                     KPISVPVMPSSKAKEEKPKAQQVQPKQEKAVDPEKEAKRKEEAELRRKQEELAQQKAE
                     MEAKRAAENARRLAEIAREEAEDNGNEDFDDERFTSSYAREAERDNDRRSEGNRGRNG
                     KGNVAKAKKGGREEDKNERSADRRNHKEVKGKGKQGKKGAALQQAFTKPAQVVKADVV
                     IGETITVAELANKMAVKATEIIKMMMKMGEMVTINQVIDQETAQLVAEELGHKVILRK
                     ENELEEAVLEDRDTHAEKVTRAPVVTIMGHVDHGKTSLLDYIRKAKVAAGEAGGITQH
                     IGAYHVETDDGKMITFLDTPGHAAFTSMRARGAKATDIVVLVVAADDGVMPQTIEAIQ
                     HAKAAGAPLVVAVNKIDKPEANPDRVEQELLQHEVISEKFGGDVQFVPVSAKKGMGID
                     DLLEAIILQSEVLELTAVKDGMASGVVIESYLDKGRGPVATILVQSGTLNKGDIVLCG
                     FEYGRVRAMRDENGKDVESAGPSIPVEVLGLSGVPAAGDEATVVRDEKKAREVALYRQ
                     GKFREVKLARQQKAKLENMFSNMAAGDVAELNIIVKADVQGSVEAISQSLMELSTDEV
                     KVKIVGSGVGGITETDATLAAASNAIMVGFNVRADASARRVIENENIDLRYYSIIYEL
                     LNDVKAAMSGMLQPEFKQEIIGLAEVRDVFRHPKFGAIAGCMVTEGIVKRNNPIRVLR
                     DNVVIFEGELESLRRFKDDVSEVRNGMECGIGVKNYNDVKVGDQIEVFEVVEVKRTI"
     gene            10166..10546
                     /gene="rbfA"
                     /locus_tag="PXE44_000417"
     CDS             10166..10546
                     /gene="rbfA"
                     /locus_tag="PXE44_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL5587962.1"
                     /translation="MSREFKRSDRVAQELQKEVAVILQREVKDPRIGMVTVSDVEVSR
                     DLAYAKIFVTFLFDNDAEAIKQGMQGLEKASPYIRTLLGKAMRLRIVPELRFIYDESL
                     VEGMRMSNLVSSVIKNDEEKHKED"
     gene            10549..11472
                     /gene="truB"
                     /locus_tag="PXE44_000418"
     CDS             10549..11472
                     /gene="truB"
                     /locus_tag="PXE44_000418"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL5587963.1"
                     /translation="MSRPRKRGRDIHGVFLLDKPQGMSSNDILQKVKRIFQANKAGHT
                     GALDPLATGMLPICLGEATKFSQFLLDSDKRYLVTAKLGERTDTSDAEGQVVETKAVA
                     VTKSQILAALEKFRGDILQVPTMFSALKHNGKPLYEYARAGITVEREARPITIFELNF
                     IEYKAPFLTLEVHCSKGTYIRTLVDDLGEELGCGAHVTMLRRTAVADYPIEKMMSYAD
                     LQNLAENQPLAELDQHLLPMDTAVSALAKINLSSEQTKAVGFGQRIRFDNLNQLYGLV
                     RLYSEDEQFLGVAEITNDTVIRPNRMINLTE"
     gene            11475..12555
                     /locus_tag="PXE44_000419"
                     /pseudo
     CDS             11475..12555
                     /locus_tag="PXE44_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
     gene            complement(12593..13117)
                     /gene="tadA"
                     /locus_tag="PXE44_000420"
     CDS             complement(12593..13117)
                     /gene="tadA"
                     /locus_tag="PXE44_000420"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL5587964.1"
                     /translation="MFIKPNQTSCPLQISEQDIAFMQYALDLADLAEAKGEIPVGAVL
                     VDKNQQIIGRGWNQTIQLCDPSAHAEMQAIRQAGQTLGNYRLLDCTLYVTLEPCPMCA
                     GAILHSRIKRLVFGASDYKTGAVGSRYHLFEDYKMNHFLEVHGNVLGRECSQKISDFF
                     KQRRLQKKGDALIK"
     gene            complement(13199..15007)
                     /locus_tag="PXE44_000421"
     CDS             complement(13199..15007)
                     /locus_tag="PXE44_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL5587965.1"
                     /translation="MTTESLTFADLGLPQPILDAVNEMGFVNPSPIQQECIPHLLAGR
                     DVLGMAQTGSGKTAAFSLPLLAQIDPTQRHPQMLVMAPTRELAIQVADACEQFAKNMK
                     GIRVVTVYGGQRYDIQLRALKQGSQVVVGTPGRILDHIRRGTLDLSELKFIVLDEADE
                     MLRMGFIDDVETVMAELPETHQTALFSATMPEPIRRITRRFMKDPQEVKIKATERSAP
                     DIEQSYWLVNGFRKNDALLRFLEVEEFDAAIIFTRTKTGTIDITELCERNGYRTAALN
                     GDMTQQAREQTLDKLRSGRLDILVATDVAARGIDIERISLVINYDIPLEEESYVHRIG
                     RTGRAGRSGRALLFVEPRERRLLRNIENLIKKPINEVAIPNHEILMEKRREKFKARIS
                     KQLEHHDLEKYRELLEDLFTADQDHEELAAAMMMLLQEKQKLILPPDPVVRAARSERG
                     RDRNERNDRRNGREHRENNGVAMDLYRIELGREDGVEVRHIVGAIANEGDISSRYIGH
                     IKLYDKYSTIELPQGMPNHLVQHFAHKARVLSKPMQMTLLGSANSASNANPFEGRGRR
                     GNDRNDRGGRRDGGFNKDRKGGFKEKRFSEKRGRRD"
     gene            complement(15209..16132)
                     /gene="nlpI"
                     /locus_tag="PXE44_000422"
     CDS             complement(15209..16132)
                     /gene="nlpI"
                     /locus_tag="PXE44_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDL5587966.1"
                     /translation="MVQLFKLFRIRFLLLNLISVLMLSGCTSRYADLVSIKNLALAEL
                     NPQLRFEQEAMVVRLSQVLEEAPLTPSEQADLYFERGVIYDSLGLWSLARYDFAQAIS
                     LNQRMAAAYNYMGLYLLLEGDYDSSIDAFNAVLELDREYSYTYLNRGLAFYYSGRYSE
                     AQRDLLHFYEEDKKDPYRALWLYFNELEFTPTEAKNNLQKRANNLSSDYWGTNIVNYF
                     LGKLNLAQVRAIMERDAKENSSQYAEILTETYFYLAKQQLKLDHKDEAISLFRLALAN
                     QVFNFVEYRFALFELSQIRGMTQTTPVVDKE"
     gene            complement(16213..18363)
                     /gene="pnp"
                     /locus_tag="PXE44_000423"
     CDS             complement(16213..18363)
                     /gene="pnp"
                     /locus_tag="PXE44_000423"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL5587967.1"
                     /translation="MTPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVV
                     AKKDVKEGQDFFPLTVDYQERTYAAGRIPGGFFKREGRPSEGETLIARLIDRPVRPLF
                     PEGFFNEIQVIATVVSVNPQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQF
                     VLNPTTSEQKISRLDLVVSGTDKAVLMVESEADILTEEQMLAAVVFGHEQQQVVIENI
                     KEFVKEAGKPRWDWVAPEPNTDLINKVKALAETRLGDAYRIVEKQVRYEQIDAIKAEV
                     IAQLTAEDETVSEGTIIDIITALESQIVRSRIIAGEPRIDGRTVDTVRALDICTSVLP
                     RTHGSALFTRGETQALAVATLGTERDAQIIDELTGEKSDRFLFHYNFPPYSVGETGRI
                     GSPKRREIGHGRLAKRGVLAVMPTAEEFPYVVRVVSEITESNGSSSMASVCGASLALM
                     DAGVPIKAAVAGIAMGLVKEDEKFVVLSDILGDEDHLGDMDFKVAGTRTGVTALQMDI
                     KIEGITPEIMRIALNQAKGARMHILGVMEQAIPAPRADISDYAPRIHTMKIDPKKIKD
                     VIGKGGATIRALTEETNTSIDIDDDGTVKIAATDGNAAKAVMARIEEIVAEVEVNQIY
                     NGKVTRVVDFGAFVSILGGKEGLVHISQITNERVERVADYLTVGQEVQVKVVEIDRQG
                     RIRLTMKDINNTNEANAEETVAENVVETEQENNF"
     gene            complement(18625..19905)
                     /gene="phoR"
                     /locus_tag="PXE44_000424"
     CDS             complement(18625..19905)
                     /gene="phoR"
                     /locus_tag="PXE44_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDL5587968.1"
                     /translation="MKVKLSLKHFLLELLLAAAVAGVFNLFFPAYFFWFSFCLLVLLI
                     WHHHTESSLLQLLRPNEQSGRSKVTTMEHLSQSAAFYHERLRQDKIKTLRLLSKLNKH
                     IQYLPDAIIICQENGEISWCNNVAQELFEFYWDKKSNKSIFNVIFYPEFKSYFRQQRH
                     HRPLVLLTHNKRYIELNLNRYDSNSRLIVARDVTSFVRLMHSRQTFLANMNHELRTPL
                     TILQGYLEMLENEPHNMALQQKALNAMKEQSQRMTNLLRQLSLLVKIEHSNDSEHNEV
                     QMSALISAIQKNTEILSQNQQILFDIEPNLTVWGDQEQLQSAVSNLIYNAIRHAGEGV
                     TIQICCQRDNQEPNTAIFSVQDDGIGIDAHHITHLTERFYRVDESRSNKTGGSGLGLA
                     IVKHALEQHHSTLEIESEAGKGSRFSFKLPLIKK"
     gene            complement(19908..20594)
                     /gene="phoB"
                     /locus_tag="PXE44_000425"
     CDS             complement(19908..20594)
                     /gene="phoB"
                     /locus_tag="PXE44_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HDL5587969.1"
                     /translation="MNNKILIVEDERAIREMLSLYLSKQYQVIEAEDYQSAINKLGEK
                     PELILLDWMMPGRSGIHLIKYLKKQPETEKIPIIMLTARSSEEDCITGLNAGADDYIT
                     KPFSPQVLLARIEAVWRRSYDPPSNLINIDGLILDQQAQRVLVAEQEVKLSGTEYKLL
                     HFLMTHPEKVYRREQLLDFIWGNDIYVEDRTVDSYIRRLRKSLEPYGFERYIQTVRGS
                     GYRFSNHFQD"
     gene            complement(20678..21448)
                     /gene="pstB"
                     /locus_tag="PXE44_000426"
     CDS             complement(20678..21448)
                     /gene="pstB"
                     /locus_tag="PXE44_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDL5587970.1"
                     /translation="MLPTDLETKLHINNLNFYYGDFHALKNINMRIAKNKVTAFIGPS
                     GCGKSTLLRTLNRMFELYPNQRATGEINLDGENLLTSDTDISIIRAKVGMVFQKPTPF
                     PMSIYDNVAFGIRLFEKLPKSELNDRVEWALTKAALWNEVKHKLNQNGDSLSGGQQQR
                     LCIARGIAVKPEVLLLDEPCSALDPISTMKIEELINELKHDYTVAIVTHNMQQAARCS
                     DYTAYMYLGELIEFGDTKQIFDKPKFRQTEDYIKGKMG"
     gene            complement(21458..22306)
                     /gene="pstA"
                     /locus_tag="PXE44_000427"
     CDS             complement(21458..22306)
                     /gene="pstA"
                     /locus_tag="PXE44_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL5587971.1"
                     /translation="MKNYQNRRFYCRKIKNVLMLSLAYSAVLFGLFWLVWIIVTLLQK
                     GIPALSWELFTRPTPAPNETGGLRNAIVGSILIVSTATLIATPIGILAGTYLSEYGRY
                     TKLAKFTRFLNDVLLSAPSIIIGLFIYAIYVAQVKHYSGWAGSLALAVLVIPIVVRTT
                     DSMLNLVPNNLREAAAALGCPRWRVITMISYRAARSGIITGVLLAVARISGETAPLLF
                     TALSNQFASLDMNAPMANLPVVIYQYAASPFADWNTLAWAGAMLITLFVLSLNIITRL
                     YFSKTK"
     gene            complement(22303..23262)
                     /gene="pstC"
                     /locus_tag="PXE44_000428"
     CDS             complement(22303..23262)
                     /gene="pstC"
                     /locus_tag="PXE44_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL5587972.1"
                     /translation="MHKHKIDNKCLNHPWVEGLFHHTTRFFALLVFALLVVVLVSLLL
                     GSWEALSRFGVSFLWENEWNPVNDRYGALVPIIGTLISAGIALVIAVPVSFGIATFLT
                     ELAPQWLKRPVGTAVEMLAAIPSIIYGMWGLFVFVPLFQEYVQPYLIDTFGQLPFIGM
                     LFSGAPFGIGLFSAGLVLAIMIIPFIASIMRDVFSVVPPLLKESAYGLGSTTWEVIWK
                     VVLPYTKTGVIGAIMLGLGRALGETMAVTFVIGNAFMLPDSVFSPSTSIASAIANEFN
                     EAGGLQKSALMSLGLILFLITSIVLAASRLLIARMGKQEGKRE"
     gene            complement(23343..24380)
                     /gene="pstS"
                     /locus_tag="PXE44_000429"
     CDS             complement(23343..24380)
                     /gene="pstS"
                     /locus_tag="PXE44_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL5587973.1"
                     /translation="MAKRFKLLTVLGISFSLSVAANAQTITGAGASFPYPIYAKWAGL
                     YEKETGNKINYQSIGSGGGQQQIIAKTIDFGASDDPMKGETLAEHKLLQFPAIIGGTV
                     PVVNIPELKSGELKLSGKVLADIFLGKITQWNDPAIVALNQDIALPAKTILVVRRSDG
                     SGTTFGWTNYLSKVSAEWKEKVGEGKSVKWPVGQGGKGNEGVAAYVKQLKYSIGYVEY
                     AYAKQNALAWVSMQNADGKFVQPSRETFAAAAANADWSQTAGMGVILTNEAGEKAWPI
                     TAASFILIHQHSDKPEQTKGVFKFFDWAFKHGKDAAAELDYVPLPDNVVEKIQTQWKT
                     EVKDGQGNSLY"
     gene            24565..26811
                     /gene="parC"
                     /locus_tag="PXE44_000430"
     CDS             24565..26811
                     /gene="parC"
                     /locus_tag="PXE44_000430"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL5587974.1"
                     /translation="MTTEINYEGIEQMPIKRFTEDAYLNYSMYVIMDRALPFIGDGLK
                     PVQRRIIYAMSELGLNASAKYKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYRY
                     PLVDGQGNWGAPDDPKSFAAMRYTESKLSKIAEILLGELGQGTVDYQPNFDGSLEEPK
                     YLPARLPHILLNGTMGIAVGMATDIPPHNINELADASVMLLDNPKATLDDVLSVVQGP
                     DYPTEAEIITPKAEIARMYEQGRGSIKMRAVWKKEEGEIVISALPHQASPSKIIEQVA
                     TQMRNKKLPMVDDIRDESDHENPIRIVIVPRSNRIDFDALMDHLFATTDLEKSYRVNM
                     NMIGLDGKPAVKNLLTILNEWLSFRRTTVTRRLNYRLDKILNRLHILDGLMIAFLNID
                     EVIEIIRNEDEPKAELMARFNLTDVQAEAILNLRLRHLAKLEEHQLQAEKSELEKERD
                     ELQLILGSERRLNSLIKKEIQADAKAFASPRRSPLVERAESKAIAESDLTPTEDVTVI
                     LSEKGWVRCAKGHDIDVQALSHRAGDGYLAHARGRSNQPVVFLDSTGRAYALDPTSLP
                     SARSQGEPLTGKITLPEGAVVQQVLMASPETKVLMASDSGYGFICTFADLVSRNKAGK
                     AVISLTENAKVLPPQLLENDENLSLVAMSNVGRMLVFPVSELPQLSKGKGNKIINISA
                     AAAKSGDEYLARLLVIKPTNSLVFVSGKRKITLKPSDVDNYRGERARKGSQLVRGLST
                     NSTVEIVE"
     gene            complement(26882..28234)
                     /gene="lysC"
                     /locus_tag="PXE44_000431"
     CDS             complement(26882..28234)
                     /gene="lysC"
                     /locus_tag="PXE44_000431"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDL5587975.1"
                     /translation="MSHLSVAKFGGTSVANFDAMTSSANIVIADANTRVVVLSASAGV
                     TNYLVELANGCEKEHRDEVIAAVRQIQYNIIEKLQHQDAVKAEIDELLERIETLAESA
                     SLATSAALSDELISHGEMMSTKIFTQLLIERGHPAVWVDVRDVVATNSHYGKAAPNDE
                     KTQQQSDNVIKPLIASGKIVITQGFIGRDDEGKTTTLGRGGSDYSAALLAEVLNANDV
                     LIWTDVPGIYTTDPRVVPNAQRIDTMAFNEAAEMATFGAKVLHPATLLPAVRSNIPVY
                     VGSSKAPEQGGTWVTRDPQPRPTFRAIALRRNQILLTLSSLSMLHAQGFLANVFAILA
                     KHKISVDVITTSEVSVALTLDKTGSASSGADMLSAELLDELNAYCNVQVESDLALVAI
                     IGNNLHAQSGVAKKLFHTLENYNIRLISYGASSNNVCTLVKNAEADDVVRSLHKGLFE
                     "
BASE COUNT         8572 a         5591 c         6375 g         8073 t
ORIGIN      
        1 atttttggta gaaataatat gactaaatct gaattaattg agagtttagc atctaaaaat
       61 ccaagtttac caattaaaat ggtggagcat tgtgtaaaag agcttttaga gcagttaacg
      121 gctactttag aagaaggtga aaggatagaa gtaagaggat ttggtagttt ttctctacat
      181 tatcggcagc cgcgtttagg tcgcaatccc aaaacaggag aaagtgttct tttaggcgcg
      241 aaatatgtac cacattttaa agctggtaaa gatctaaaag agagagttga tctgctttaa
      301 gtcttattaa ttcaataatc gtagtaagga gattatatga ttaagtatat tttaggtatt
      361 ttaattgtga ttgcagttat tattgtcgct gttacgattg gagccaataa tgatcaactt
      421 atcaccttta actatatttt tgctcagagt gaattacgct tatcaacgtt agttgcgatt
      481 ttatttggct taggattgat tcttggttgg ttggtaacgg gtatttttta cttaaaaatt
      541 aagtttcaaa acgttgcttt aaatcgtcgt gtaaagcgcc aagctcagca aataactgaa
      601 ttaacgactc caaaggctga ataatcaatg cttgaattat tgttcctttt attgcctatt
      661 gcagcactat atggctggta tatgggacaa cgtagtgcaa agaaggatca agaaaccgct
      721 agtaataagt tctctcgtga gtatgttacg gggttaaatt ttcttttatc taatcagcag
      781 gaaaaggcgg tggacctttt cctctctatg ttacaaaaac aagaatccga aaatcatatt
      841 gcatctgaat cccaatttga agcggagtta accctaggta atcttttccg ttcaagaggt
      901 gaggtagatc gtgccttacg tattcaccaa acattagtga atagccctca ttattcattt
      961 gatcaaaaat tattagcaaa acagcaatta gctaaagact ttatggttgc aggtttctat
     1021 gaccgtgcag aaaattatta catttcatta gttgatgaac ctgagtttgc taaatattct
     1081 ttatctcagc ttgctgttat ttaccaaaaa accagagaat ggaaaaaagc gattaatgtt
     1141 acagaaaaac ggcttcgaat agagccggat gcagataaaa taccgctttc acatttttat
     1201 tgtgaatatg cgcaaggggt taaatttgat gataaagcgg catttattga atcgttaaat
     1261 aaagcgttag aatattatcc tcaatccaca cgcgcttcta taatattagg tgatttctat
     1321 ttagaagagg gagaatatct aagagcatta agctatttcg aacagattct tgtgcaagac
     1381 cctgattata ttagcgaagt gcttggacga attaagcaaa gctatatggc tcttgaggac
     1441 ttaaatggct atgagttatt tctcattcga gcaaaccgag taaaacataa tagtagtgta
     1501 gatattgctt taactgaatt tattgaagaa aaagatggta tcaatgctgc acattctaaa
     1561 ctataccagc agttgagtac ttatccgaat ttaattactt ttcaccgctt tattcgctat
     1621 caagcagatt ttgcggagga aggaaatggt aaggaaagct tagttttatt acataatatg
     1681 gtgggaaatc agataaaacg cagcttccaa tatcgctgtt taaattgtgg ttatcaaagt
     1741 tatcgcttaa tgtggcaatg tccttcttgt aatcaatggg aaaaaattaa gccggtacaa
     1801 agtatcgaag gtattattca ataaagaaag aatctgagga attgaaaatg aacaataaag
     1861 taattgttgc gttagactat gaaactgaac gtgaagcatt agaatttgtg gatttggtcg
     1921 atccgtcgct ttgtcgttta aaagtaggca aagaaatgtt tacaacctta ggcacaaatt
     1981 ttgtcaaaca attacacgaa cgtaaatttg atgtattcct tgatttaaaa tatcatgata
     2041 ttccaaatac ggttgctcga gcagtacgtt ctgctgctga tcttggtgtg tggatggttg
     2101 acttgcatgc ttctggtggt ttagccatga tggaagaggc aaagaaaatt ttggagcctt
     2161 atggcaaaga ggcaccatta ttaattgcag tgacggttct aaccagtatg gaagatttgg
     2221 atttgcttca aataggtatt aatgcttcgc cgatggaaca agttattcgt ttagcacatt
     2281 tagcacaacg ggctggttta gatggcgtgg tatgttctcc acaagaagtt gaggtattac
     2341 gtacccattg tggtcaagat tttaaattag tgaccccagg aatccggcca aaaggaacag
     2401 attttgggga tcaacgccga gtgatgacac ccaaacaagc agtagaaaca ggctcagatt
     2461 ttttggttat tggtcgtcct attacccaag ctcaagatcc gcttgcggta ttaaaatcta
     2521 ttaaccaatc tatcagttaa ttcattgcat ttttaatgaa ggcggaaaaa tttccgcctt
     2581 ttttaatcaa acaagcggtt atttttgagg aaaaatttgt aaatgacatt agtctattca
     2641 acggaaacag gcagaataaa gtccgaagta ataaaagaag aacgccctaa aggggatggt
     2701 attgtgcgta ttcaacgtca aacaagcggt agaaagggga aaggtgtttg ccttatcacc
     2761 ggtcttgatt tagaggataa agccttaaat tctcttgctt cagagcttaa acgtaaattg
     2821 ggttgtggtg gctcatttaa agatggagta attgaaatcc agggcgataa tcgaggattc
     2881 attaagcaaa tcttagaaca gaaaggtttc aaagtgaaac tggcaggtgg ttgagtttta
     2941 aacaattatc agctggcagt ttttaaataa cttcatttta tttgaaactt cattcctact
     3001 tttctgccta aaactcacga ttaaggagaa aaactaatgt ggaagaaaac gttgattgct
     3061 gtatttttag caagccaata tgcttttgcg gtaggaaccg ggaaagaacc atcaaaaagt
     3121 tatactgaaa cagaattaaa acaattaaaa aatgattggt tagcgattta tcaaaaagag
     3181 ctacagtatg cagaaaattt gcgtttaaaa cagagagaaa attttcagca aattaattat
     3241 ttggcagatg cagcgattag tcagaagaat ttatcacttt caacggagca gataattcag
     3301 cgtttattac taaccttagc gggttatccg ttggagatga aagctgaatg gaagctgcta
     3361 aatgctaaaa taacgttaaa acaagccaca gaaagtgaag ttaaagcatt tatttcaaaa
     3421 tatcctgatt ctttgtatca aaggcagtta cagaacttga tgtttgagct gctctatcgt
     3481 gaacagaaat ttccggagtt actggaatat gctcaaaatg taaaacagag tagtcttgcc
     3541 gaccaatgcc gtttattttc tgctcgttat gaactggcag caagtaaagc tcaaatcaat
     3601 ccggaagttt ctcaagtaac tgagcaaaaa gcagaaaatg cagaactgct tgctttggtg
     3661 caagagtttg atcgcttttg gcttgctacg ccgaaattaa cttctgattg tgcgaattta
     3721 gaaagttatt ggcgggatca ggggcttaaa accgatgaaa aaatgcgttt gaaagcggtc
     3781 gaattaatta aacaaaatgc aaatgcagaa ttggcgaatt tggcggcaaa tgtaaataat
     3841 gagaatttga aaatctggct atcttccgtt gaaaaggtag cgaataatcc ggcagatttg
     3901 caaaatttta tcgaaaatca accgcttgat tccccatttt atgctgataa taaagcgatt
     3961 attcagcaac tctttgctaa atatgtgcga actttatcgg aaaatatgga gaacccgagt
     4021 ttccagcagt atcaaacgtg ggcagagaag tatcaacttt ccagcgagga aatcaatggc
     4081 tggaaaaatg cttttatcac tcgatttttt gataacccag aaccaacatt ccaattttgg
     4141 cgtgatgagc aaatcaagtt gctcaagaca gacaatttaa ctgaacgccg tttacgaatg
     4201 gcaatttggc aaaaagcaga tttaaatgag tggctggttc tgctatctca gcaggcgaaa
     4261 gaaaaagcag aatggcgtta ttgggctgcg aaagaggaga aaaacgaagc gaagcgtaaa
     4321 gttgattttg aaagcttagc taaagagaga ggcttttatg ctatgttggc tgctcaacaa
     4381 ttaggcattg tttaccaaat cccgatgctt gaagtgcctg aattgacaca agcacagctt
     4441 gaattgtatg caaaccaatt agctcgtatt caagagttgc gagagctagg gcagtttgac
     4501 caggcaagaa aaatatggat tgactggata aaaaacatca agccggaagc aggtgaaaat
     4561 acaccaacta aaatccagtt agccctgata aaatatgcca aggataaaaa ttggtatgat
     4621 ttggcggtgg aaggtacaat tcaaacaaaa gcctttgatt atcttgattt gcgattaccg
     4681 aatgcctatt cggattggtt cgatttacat ctgcaagata aaaaaatcag caaaacgttt
     4741 gctcaggcga ttgctcgtca agaaagtgcg tggaatgctc aggctcaatc gcacgctaat
     4801 gcgagagggc taatgcaact gttaccgaca acagcacaaa aaaccgctaa agataacggt
     4861 ttaaccttca aagatggcaa tgatctcttt caaccgttta acaatattat gttaggcaca
     4921 acccatttaa tggagttaaa cgaaaaatat ccgaataatc ggattctgat tgcctctgct
     4981 tataatgcag gagcagggcg tgttgagcag tggctgaaac gtagtaacgg gcagttagca
     5041 atggatgaat ttattgccag tatcccgttt tatgaaacca gaggctatgt acaaaatgtg
     5101 ctggcgtatg actactatta tcaaatgctc tatagcgata taaatgataa aaatggatta
     5161 aaaatgttct atcaagagga attaacaaga aaatattaaa aaatgcttgt gtttttgtgt
     5221 agaaccgcta taatgcaccc catcagacgc ggggtggagc agcttggtag ctcgtcgggc
     5281 tcataacccg aaggtcgttg gttcaaatcc ggcccccgca accagtttta agctcacagc
     5341 tttggttaaa gttgtgagtt ttgttttata tgctgtagca aatttgcaaa aaatttggca
     5401 aaaaagaccg cttgttgtgg ttgctacagc aaaaaacaaa ccgcttaaag ccccacggtt
     5461 tcccgtctgg ggcttttttg cggaaattgg tacagcatta acgtatcaaa gctggtttat
     5521 ccggcttttt ttatatttgg agattatgac ttggcaactt tagaacaaaa attagaagat
     5581 ttagtgctcg attctattga agcattaggc tttgaattag tcggcattga atgccaacgt
     5641 gcaggccgtt ttttaacggt gcgtttatat attgataaag aaggtggcgt gacgattgat
     5701 gattgtagcg atgtcagccg ccaagtgagt gcgatttttg atgtggaaga tccgattgcc
     5761 gataaatata acttagaggt ttcttcacca gggttagatc gtccattatt taccgttgcc
     5821 cactatgagc gttttatcgg gcaagatgta gtgattcatt tacgcattcc gatgttcgat
     5881 cgccgtaaat ggcaaggtaa aatcaaacaa gtagaaggtg atttcatcac tttaacggta
     5941 gaaaatgaag atcgccagtt cgcatttggc aatattcaaa aagcaaattt agttccggtt
     6001 tttaattttt aagataaagg taaactgaca aaatgagtaa agagatttta ttagctgcgg
     6061 aagcagtttc aaacgaaaag caattaccaa aagaggcaat ttttgaagcc ttagaaaccg
     6121 ctttaaccat tgcaaccaaa aagagccgtg atttagatat tgacgtgcgt gtggtggtag
     6181 atcgtaaaac cggtgatttc caaactttcc gtcgttgggc tgtggttgaa aatgtgaata
     6241 atatgacccg tgaaatcagc ttagaagcgg cacaatttga agatccaaac gtacaattag
     6301 gcgattttat tgaagatgaa gtagagtcta tcgagttcgg tcgtgtggcg attcagactt
     6361 ttggtcaggt agtcaaaacc aaaattcgtg aagcggagaa gacgaaaatc attgatgaat
     6421 tccgttcgga tttaggcaaa attattcttg ccactgtgaa aaaagtaacc cgtgataata
     6481 ttttccttga gttacaaggt aaatcagaag atatcaaagg cgaagcggtc attgtgcgtg
     6541 aagatatgat cccacgtgaa aacttccgcc cgggtgaccg tgtgcgtggt gtgttatacg
     6601 aaatcaagcc tgaatcaaaa ggacctcagc ttttcgttac ccgtgctaaa ccggtgatgt
     6661 tagaagagct attcaaattg gaagtgcctg aaattggcga aggcgtaatt gagatcaaag
     6721 gttcagcccg tgatgtcggc tctcgtgcaa aaattgccgt gaaaacccac gataaacgta
     6781 ttgacccggt aggggcttgt gtgggtatgc gtggtgctcg tgttcaagct atcagcaatg
     6841 aattaggcgg cgagcgtgtg gatatcgtac tttgggacga aaatccggct caatttgtga
     6901 ttaatgcaat ggctccggca gatgtaagct ctattgtgat taacgatgaa actcactcaa
     6961 tggatattgc ggttgaagcg aaaaatctgg ctcaagctat cggtcgtaac ggtcaaaacg
     7021 tgcgtttggc gacacaatta actggttggg cattaaatgt aatgaccgtt gaagagctaa
     7081 cgaagaagag cgaagaagaa gatggtaaag tcatcaattt attaacttct gctttagaaa
     7141 ttgatgaaga atttgctcaa gttttaattg aagagggctt tacgtctctt gaagcgattg
     7201 cttatgtgcc ggttgatact ttaacggcga ttgacggttt agaagatgaa gatttagtcg
     7261 aagaattaca atctcgtgcc aaaaatgcga tcacgaaaaa agcgttagaa gaagagaagt
     7321 tattaaaatc agctcaaatt gaagaaaaat tattaacgct tgaaggtatg gatcgccata
     7381 tcgcattcaa attagctgaa aaacagatta cgactcttga agaacttgca gagcaaggtg
     7441 ttgatgattt agccgatatt gaagaacttt cagaagagaa agctggcgag ctaattatgg
     7501 cagcacgcca aatttgttgg ttcaactaag caaagaggat aataaaatga cagaagagaa
     7561 aaaaacactt tcgcttggtg gcgctcgcaa agcgagtaaa gtgaatacaa ccacaaccag
     7621 tggtaaagta aaagcggttg aagtaaaaga gaaaaaaccg aaaattgatg caaaagcatt
     7681 gcaagaaaaa gcagcaaaag aagctgccgc agcgaaagct agagctgaaa aagaagcagc
     7741 tgaaaagtta gccaaagaaa aagccgaagc tgaagctaaa gagaaagctg caaaaaaagc
     7801 agcagaatcg accgctagta aaccgatttc tgtgccggta atgccaagca gcaaagcaaa
     7861 agaagaaaaa ccgaaagcac aacaggtaca gccaaaacaa gagaaagcgg ttgatcctga
     7921 aaaagaagct aaacgcaaag aagaagctga gttacgccgt aagcaagagg aattagctca
     7981 gcaaaaagcg gaaatggagg caaaacgtgc tgcagaaaat gcccgccgtt tagcagaaat
     8041 tgctcgtgaa gaagcggaag ataacggcaa cgaagatttt gatgatgagc gttttacttc
     8101 ttcttatgct agagaggctg agcgtgataa tgaccgccgt agtgagggaa atcgtggtcg
     8161 taacggcaaa ggcaatgtgg ctaaagcgaa aaaaggcgga cgtgaagagg ataaaaacga
     8221 acgcagtgca gatcgtcgca accataaaga agtaaaaggc aaaggtaaac aaggtaagaa
     8281 aggtgcagct ttacaacaag cctttaccaa accagctcaa gttgttaaag ctgatgtggt
     8341 gattggtgaa actatcactg tggctgaatt ggctaataaa atggcagtga aagcgactga
     8401 aatcatcaaa atgatgatga aaatgggtga aatggtgacg atcaaccaag ttatcgacca
     8461 agaaaccgca caattagtgg cggaagaact tgggcataaa gtgattttgc gtaaagaaaa
     8521 tgagttagaa gaagcggttt tagaagatcg tgatactcac gcagaaaaag taacccgtgc
     8581 accggtagtg actattatgg gccacgttga ccacggtaaa acctcgttac ttgactacat
     8641 tcgtaaagcc aaagtggcgg caggtgaagc aggtggtatt acccagcaca ttggtgcata
     8701 ccacgttgaa accgatgacg gcaagatgat cactttctta gataccccgg ggcatgcagc
     8761 ctttacctca atgcgtgcgc gtggtgcgaa agcaactgac atcgttgttc tcgtcgttgc
     8821 ggctgatgac ggtgtaatgc cacaaactat tgaagctatc cagcacgcca aagcagcggg
     8881 tgcgccatta gtggtagcgg tgaataaaat tgataaaccg gaagcgaacc ctgaccgtgt
     8941 agaacaagag ttactacaac acgaggttat ttctgagaaa ttcggtggcg atgtgcaatt
     9001 cgtgccggta tctgcgaaaa aaggtatggg gatcgatgat ttactagaag ccattattct
     9061 gcaatccgaa gtgcttgaat taaccgcagt aaaagacggt atggcaagcg gcgtggtaat
     9121 cgaatcttac ttagataaag gtcgtggccc tgtggcaact atcttggttc aatccggtac
     9181 gctaaacaaa ggcgacattg ttctatgtgg ttttgaatac ggacgtgtgc gtgcaatgcg
     9241 tgatgaaaac ggtaaagatg ttgaatcagc aggtccgtct attccggtag aagtattagg
     9301 cttatcaggc gtgcctgctg ccggtgatga ggcaaccgtc gtgcgtgatg agaagaaagc
     9361 ccgagaagtg gctctttatc gccaaggtaa attccgtgaa gtaaaactgg ctcgtcagca
     9421 aaaagctaaa ctggaaaata tgttcagtaa tatggcagcc ggtgatgtgg cagaattgaa
     9481 catcattgtt aaagcagacg tacaaggctc ggtagaagcg atcagccaat ccttaatgga
     9541 actttcaacc gatgaggtga aagtgaaaat tgttggttca ggtgtaggtg gtattaccga
     9601 aactgatgca accttagccg cagcttctaa tgctattatg gtcggcttta acgtgcgtgc
     9661 cgatgcctct gcccgccgtg tgattgaaaa cgaaaatatc gatttacgtt actactccat
     9721 catttatgag ctattaaatg acgtgaaagc agcaatgagc ggtatgttac agcctgaatt
     9781 caaacaagaa attatcggct tggcagaagt gcgcgatgtg ttccgccacc cgaaatttgg
     9841 ggcgattgcc ggttgtatgg tgacagaagg tatcgttaaa cgtaacaacc caatccgtgt
     9901 tctgcgtgat aacgtagtaa tttttgaagg cgagttagaa tctctccgcc gtttcaaaga
     9961 tgacgtatct gaggtgcgta acggtatgga atgtggtatc ggcgttaaaa actacaacga
    10021 cgtaaaagtt ggcgaccaaa tcgaagtgtt tgaagtggtt gaagtaaaac gtactattta
    10081 gcctaatttt gtgtaggggc gtattgcata cgccctttgt attttttagg gcgtgaagca
    10141 tcacgcccct ataaaagaga aaaatatgtc cagagaattt aaacgtagtg accgtgtggc
    10201 acaggagcta caaaaagaag tggcggtgat tttacaacgt gaggtaaaag atcctcgcat
    10261 tggtatggtg actgtttctg atgtggaagt atcaagagat ttggcgtatg ccaaaatttt
    10321 tgtgactttc ttatttgata acgatgctga agctattaag caaggtatgc aaggcttgga
    10381 gaaagcaagc ccctatattc gaaccctttt aggtaaagca atgcgtttgc gtattgtgcc
    10441 tgaactgcgt tttatctacg atgaatcgct agtcgaaggt atgcgtatgt ctaaccttgt
    10501 gtctagtgta atcaaaaatg acgaagaaaa acataaagag gactaattgt gtctagacct
    10561 cgcaaacgtg gccgtgatat tcacggcgtt ttcttattag ataaaccaca aggaatgtct
    10621 tcaaacgata ttctgcaaaa ggtaaagcga attttccaag caaataaagc cggacatacc
    10681 ggggcgttag accctctcgc aaccggaatg ctgccgatct gtttaggtga agcgaccaaa
    10741 ttttcgcaat ttttgttgga ttcggataaa cgttatctcg tcaccgcaaa attaggcgag
    10801 cgaaccgata cttcagatgc ggaagggcaa gtggtggaaa ccaaagcagt tgcagtgaca
    10861 aaatctcaaa ttttggctgc tttggagaaa tttagaggtg atattttgca agtgccgaca
    10921 atgttttcgg ccttaaaaca caacggcaaa cccttgtatg aatatgcccg agcagggatt
    10981 acggtggagc gtgaggctcg cccgattacg atttttgaac taaatttcat tgaatacaag
    11041 gctccatttt taacccttga ggtgcattgt tctaaaggta cttatatccg aactttggtc
    11101 gatgatttag gcgaagagtt gggctgtggt gcacacgtta cgatgctacg ccgtacagca
    11161 gtggcagatt atccgattga aaaaatgatg agctatgcag atttgcaaaa tttagccgaa
    11221 aatcaaccgc ttgcagagtt agaccagcac ttattgccga tggatacggc agtttctgca
    11281 ttagcaaaaa taaatttaag ttcagagcaa actaaagcgg tgggttttgg tcagagaatt
    11341 agatttgata atctgaacca actttatggt ttggtacgtt tatattcaga agatgaacaa
    11401 tttctcggtg tggcagaaat caccaatgat actgtgattc gtcctaaccg aatgattaac
    11461 ttaacggaat aagaatgaaa tcgataattt ttaccttacc tttagcgatt atcactttag
    11521 ccttagccgg ttgttcaagt gataatcaag aaagttatgc cggcttaaac ttagatgcta
    11581 aatacagtaa atctcgctcg gtcagtcatt ttaatgatta tgtgcatttt ctgaaacaga
    11641 aggcagcagg ggcaggagtg tctgcaaaaa ccttaaatgc tcagaaattc attcattaca
    11701 acgccagagc ggtgcagtta gatcaacagc aagctgcacg aagacgtgac ccgaatgtac
    11761 cgcctccgcc accaaatcca agttttaaac aaagtattaa ctcaagcgaa ggtggatcag
    11821 gcaagtgaac gttggtatga atataatagc cagcttaccc atgccagcca aaaatatggg
    11881 gtacaaaaag agtatatttt ggcactttgg ggaatggaga gcagctttgg gcgtaaccaa
    11941 ggggattttg atgtactttc ggtgttggca accctcgcct ttgatggtcg ccgtgagaag
    12001 ttatttacgc aagaatttgt gaatgcgatg aaaatgctcg atgacggcac aattaaacgc
    12061 tatgaattgc ttggatcttg ggcaggagcg atgggacaaa ctcaatttat gccaacttct
    12121 tacttaacct atgcagctga tggcaataat gacggtaaaa aagatatttg ggagaatgaa
    12181 gccgatgcat ttgcctcgat tgccaattat ctttcaacag taggttggga taatcaacta
    12241 ccttggggtg ttgaggttaa attagcttca ccgatggatt taagtttcgc cggtcttgaa
    12301 accaacaaag cgaaaacatt ggcggaatgg caagccttag gtatttattt agcttatccg
    12361 aatggacaag aatcggcaaa attagctcaa ttacacggca aagaggtttg gttgattcgt
    12421 ccggataaag aagcgggaag agcgttttta gtttctaata atttccgtac tttaatggat
    12481 tggaataaat cgaataacct tggtatcagt atcggaaaat ttgccgatcg aattttagct
    12541 tcagttggtc aataatcgaa taaaagcata gtagcaaagc ctatctatgc ttttatttta
    12601 tcaaagcgtc gcctttcttc tgcaaacgac gttgcttaaa aaaatcactg atcttttggc
    12661 tacattctct gcccaataca ttgccatgca cttctaagaa atggttcatt ttataatctt
    12721 caaacagatg atagcgtgaa cctaccgcac ctgttttata atcacttgcc ccaaaaacca
    12781 atcgcttaat ccgactatgt aaaattgcac cggcacacat tgggcaaggc tcgagcgtaa
    12841 cataaagggt acaatctaac agacggtaat tccctaaagt ttgtcctgct tgacgaattg
    12901 cctgcatttc agcgtgtgca gatggatcac agagttgaat ggtttggttc cagcctctgc
    12961 cgataatctg ttggttttta tcaaccagta ctgccccaac cgggatttcg ccttttgctt
    13021 cggctaaatc agcgagatca agggcgtatt gcataaaggc gatgtcttgc tcggagatct
    13081 gaagtggaca agaagtttga tttggtttga taaacatagt tgcaaaaaat tgagtttttt
    13141 tgaccgcttg taaaatgccc cacgagggtg gggcatcgct tacttcgctt agtgtcgttt
    13201 agtcacgacg accacgtttt tcactgaaac gtttttcttt aaagccacct ttgcggtctt
    13261 tattgaaacc cccgtctcgg cgaccaccac gatcattacg atcgttacca cggcgaccac
    13321 gaccttcaaa tggatttgca ttacttgcac tgtttgcaga gccaagtaat gtcatctgca
    13381 ttggtttgct taatacacgt gctttgtgag cgaaatgttg tacgagatga tttggcattc
    13441 cttgcggcaa ctcaatggtt gagtatttat cataaagttt gatatgcccg atgtaacggc
    13501 tgctgatatc cccctcgttg gcaatcgctc caacaatatg acgaacttcc acaccgtctt
    13561 cacgccccaa ttcaatgcgg tataaatcca ttgccacacc attgttttca cggtgttcac
    13621 gaccgttacg acgatcgttt cgctcattgc ggtcacgtcc acgttcagaa cgggcagcac
    13681 gcacaaccgg atccggtggc agaatcagtt tttgtttttc ttgcagtaac atcatcatcg
    13741 ccgctgctaa ttcttcgtgg tcttgatccg cagtaaataa atcttccaac aattcacgat
    13801 atttttctaa atcgtgatgt tctaactgct tagaaatgcg ggctttgaat ttctcacggc
    13861 gtttttccat taaaatttcg tggtttggaa tcgccacttc attaattggt tttttgatta
    13921 aattttcgat attgcgtaat aagcggcgtt cacgaggctc aacaaacagt aaggcacgac
    13981 ctgaacgacc ggcacgaccg gtacgtccga tgcggtgaac ataagattct tcttctaacg
    14041 gaatatcgta gttaataacc aagctgattc gctcaatatc aataccacgg gcagcaacat
    14101 cggtggcgac taaaatatct aaacgaccgg agcgtaattt atctaaggtt tgctcacggg
    14161 cttgctgggt catatcgccg tttaatgccg cagtacggta gccgttgcgt tcacacaatt
    14221 cggtaatatc aatagtaccg gttttggtgc gggtaaagat gatagcagca tcaaattctt
    14281 ctacttctag gaaacgcaat aacgcatcgt ttttacggaa gccgtttacc agccagtagc
    14341 tttgttcaat atccggggct gaacgctcgg ttgccttaat ttttacctct tgcggatctt
    14401 tcataaaacg acgggtaata cggcgaatcg gctccggcat ggtggcagag aagagagcag
    14461 tttgatgtgt ttccggtaat tcagccatta ccgtttcaac atcgtcaata aagcccatac
    14521 gcagcatttc atcggcttca tctaacacga tgaattttaa ttcggataaa tctaatgtgc
    14581 cacggcggat atggtcaaga atacgcccag gtgtacccac tactacttgt gagccttgtt
    14641 ttaaagcacg taattgaatg tcgtaacgtt gtccaccata aaccgtcact acacgaatgc
    14701 ctttcatatt ttttgcaaac tgctcacaag catcggctac ttgaatcgca agctcacggg
    14761 tgggtgccat caccaacatt tgcgggtggc gttgtgttgg gtcaatttgt gccaacagcg
    14821 gcagcgagaa tgcggcggtt ttaccgctcc cggtttgtgc cataccaagc acatcacgac
    14881 cggctaataa atgaggaata cattcttgtt gaatgggtga tgggtttaca aagcccattt
    14941 catttacagc atcaaggatt ggttgaggta agcctaaatc ggcgaaagtt aaagattcag
    15001 ttgtcataaa aaaccatttg tttggaacgc acaaagcaga aaatatcgac tatttttctg
    15061 cacagccatt aatgaaaatt atattgttga aatttaggaa gaaatgaaat aacaaagcaa
    15121 gtgatgcttt aaaaatcccc tgagtataag cggttaaatt tacaaaattt tttgcgaaat
    15181 taaccgcttg tattgtccta atctttccct attctttatc aacaacggga gtcgtttgag
    15241 tcataccacg aatttgtgat aactcaaaca gggcaaaacg atactcaacg aagttaaaca
    15301 cttgatttgc aagagcaaga cgaaagaggc tgattgcctc atctttatga tctaatttga
    15361 gttgttgttt tgctaagtag aaatacgttt ctgtcagtat ttctgcatat tgtgagctat
    15421 tttccttagc gtcacgttcc attatagcac gaacttgagc taaattgagc ttccctaaaa
    15481 aataatttac gatatttgtt ccccagtaat ccgaagaaag attattagct cgcttttgta
    15541 aattattttt agcttctgtt ggtgtaaatt ccagctcatt aaaatagagc cataatgctc
    15601 gataaggatc tttcttatct tcttcataaa aatggagaag atcacgctgg gcttcagaat
    15661 atctgccgct ataatagaaa gccaaacctc gattgagata ggtgtagcta tattcccgat
    15721 ctaattccaa tacggcatta aacgcatcaa tcgaactgtc gtaatcgcct tccagcagca
    15781 gatacagccc catatagtta taagctgccg ccatacgttg attgagcgaa atggcttgag
    15841 caaaatcata acgggctaac gaccataaac ctaaactgtc gtaaataacg cctctttcaa
    15901 aatagaggtc tgcttgctca gagggcgtta atggggcttc ttccaatact tggctgagtc
    15961 ttaccaccat tgcctcttgt tcaaagcgaa gttgaggatt aagttctgcc aatgctaagt
    16021 ttttaattga aaccaaatcg gcataccgac tggtacagcc gcttagcatt aaaacagaaa
    16081 ttaaattcag taataagaaa cggattcgaa acaacttaaa aagttgtacc attacagcat
    16141 atccctcaat taaggaaaat taaaaactga ataacggaca ctcaaagtcc gttattcgcc
    16201 tttattattg aactagaaat tattttcttg ttctgtttct accacatttt cagctacagt
    16261 ttcttctgca tttgcctcgt tggtattatt gatgtctttc atcgtcagac gaatgcgtcc
    16321 ttgacggtca atttctacca ctttcacttg tacttcttga ccaacggtta agtagtccgc
    16381 tacacgctca acacgttcgt tggtgatttg tgaaatgtgg actaaacctt ctttgccacc
    16441 taagatggaa acgaatgcac cgaagtccac cacacgggtt actttaccgt tgtagatttg
    16501 gtttacttcc acttcggcaa cgatctcttc aatacgagcc attactgctt tcgctgcatt
    16561 gccgtcagtt gccgcaattt tcaccgtacc gtcatcatca atgtcgatag aagtattggt
    16621 ctcttcggtt aaagcacgaa ttgttgcacc gcctttacca atcacatctt tgattttctt
    16681 cggatcgatc ttcattgtgt gaatgcgtgg ggcatagtca gaaatatctg cacgaggtgc
    16741 cggaatggct tgttccatta cacctaaaat gtgcattctt gcacctttag cttggtttaa
    16801 ggcaatacgc ataatttcag gggtgatccc ttcgattttg atgtccattt gcagcgcagt
    16861 cacaccggta cgggttcccg ctactttaaa gtccatatcg cctaagtggt cttcatcacc
    16921 taagatgtca gaaagtacca cgaatttctc gtcttctttc acgagcccca ttgcgatacc
    16981 ggcaaccgct gctttgattg gcacacctgc gtccatcaac gcaagagacg caccacacac
    17041 agatgccatt gaagaagaac cgttagattc agtgatttca gacaccacac gcactacata
    17101 cgggaactct tcagcggttg gcatcacggc taatacgcca cgttttgcta aacgaccgtg
    17161 accgatttca cggcgttttg gcgagccgat acgaccggtt tcgcccacag agtatggagg
    17221 gaaattgtag tggaataaga aacggtcaga tttttcgccg gtcaattcgt caatgatttg
    17281 ggcatcacgc tctgtgccca atgttgctac tgctaatgct tgggtttcgc cacgggtgaa
    17341 aagagcagaa ccgtgggtgc gtggtaacac actggtgcaa atatccaatg cacgcacggt
    17401 atccaccgta cggccgtcaa tgcgtggttc gcctgcaata atacggctac gcacgatttg
    17461 gctctctaat gcggtgatga tgtcgatgat agtcccttca gaaacagttt catcttctgc
    17521 ggtaagttgt gcaatcacct ctgctttaat cgcatcgatt tgctcgtaac gaacttgttt
    17581 ttctacgata cgataagcat cgccaaggcg tgtttctgct aatgctttta ctttgttgat
    17641 taaatctgta tttggctctg gtgcaaccca atcccaacgt ggtttgcccg cttctttaac
    17701 aaattctttg atgttttcga ttacaacctg ttgttgctcg tggccgaaca ccaccgccgc
    17761 taacatttgc tcttcggtta agatatccgc ttcagattcc accatcaaca cggctttgtc
    17821 tgtacctgaa accactaaat ctaagcggct gattttttgc tcgctggtgg ttgggtttaa
    17881 tacgaattga tcgttgataa aaccgacacg agccgcaccg attggaccgt taaacggcac
    17941 gccggataat gaaagggcag ccgatgcacc gatcatcgca accagatcag gactgatttg
    18001 tgggtttacc gatactacgg tcgcaatcac ttgaatttcg ttaaagaaac cttctgggaa
    18061 aagtggacgc acaggacggt cgatcaagcg agcgattaag gtttcacctt cgctaggacg
    18121 tccttcacgt ttgaagaaac cgcccggaat acgaccggct gcgtaagtac gctcttgata
    18181 atctacggtt aatgggaaga aatcttgccc ttcttttacg tcttttttcg ctactacggt
    18241 aacaaatacg gttgtgtcgt ccatacttgc cattactgct gccgttgctt ggcgtgcgat
    18301 agcaccggtt tctaaggtca cggtgtgctg accgtattta aactgtttta caattggagt
    18361 cattgaaatt ttccttttaa agaatttact attattgtcg ttttatttct ctaaattgcg
    18421 actagcaaaa aggcttttaa gtaaaacatt tttgatagcc gcctctcaat aacaagaaat
    18481 aaaacttgtc tagtatagcg tgatttttat aaaaaatcgc ctattatttt gatcttgcac
    18541 ctcagttata aagaaattca aaacttcagt gtaaaatggg gattagtttg tgaaaagcct
    18601 tgtgaaaata gtttgattgc atcgctattt ttttattaga ggtagtttaa aactaaatct
    18661 gcttccttta ccggcttcac tttcaatttc cagtgtggaa tggtgctgtt caagagcgtg
    18721 tttgacaata gccagaccta aaccgctacc gccggttttg ttgctacgag attcgtccac
    18781 acgatagaaa cgttctgtca gatgggtaat atgatgggca tcgataccaa taccgtcatc
    18841 ttgcacgctg aaaatcgcag tattcggctc ttgattatca cgttgacaac atatttgaat
    18901 ggtaactcct tcaccggcgt gccgtattgc attgtaaatt aaatttgata cagcgctttg
    18961 gagttgctct tgatctcccc ataccgttaa attaggctca atatcaaaca gtatttgctg
    19021 attttggctt aaaatctcgg tatttttttg tatggcggag attaacgccg acatctggac
    19081 ctcattgtgt tcggaatcat tggaatgctc gattttaacc aaaagtgaga gttggcgaag
    19141 caggttagtc atacgttgac tttgctcttt catcgcattg agcgcttttt gctgtaatgc
    19201 catattatgc ggctcgtttt ccagcatttc caaataacct tgtaatatgg taagaggtgt
    19261 tcgcagttcg tggttcatat ttgccaaaaa ggtttgtcgt gagtgcataa ggcggacaaa
    19321 agaggttaca tctcgtgcga caataagtct ggagttactg tcatatcgat tgagattcaa
    19381 ttcaatatag cgtttattat gagtcagcag taccaatggg cgatggtgtc gttgttggcg
    19441 gaaatagctt ttaaattcag gataaaaaat cacattaaaa atgcttttat ttgatttttt
    19501 gtcccaataa aactcaaata gctcttgtgc aacattattg caccaagaaa tctccccgtt
    19561 ttcttggcaa ataatgatcg catcgggtaa gtattgaata tgcttattta attttgataa
    19621 cagacgtaag gttttgattt tatcttggcg taaacgttcg tgatagaatg cggcactttg
    19681 agataaatgc tccattgtcg ttacttttga cctgccgctt tgctcattag gtctgagtaa
    19741 ctgaaggaga gaagattcgg tgtggtgatg ccaaataagt agaaccagta aacaaaaact
    19801 aaaccagaaa aaataagcag ggaaaaacag attgaatacg ccggcaacgg ctgctgccag
    19861 caaaagttcc aataaaaagt gtttaagtga gagcttaact ttcattatta gtcctgaaaa
    19921 tggttggaaa aacggtatcc cgagccacgt acggtttgaa tataacgctc aaaaccatag
    19981 ggttcaagac tcttacgcaa tcgccgaata taagagtcta ccgtgcgatc ctcaacataa
    20041 atatcattac cccaaataaa atcaagcaac tgttcacgac gatacacttt ttcagggtga
    20101 gtcattaaaa agtgaagcag tttatattcc gtgccgctta atttcacttc ctgttcggca
    20161 accaaaaccc gttgtgcctg ttgatcaaga atcaatccgt ctatattgat gagattgctt
    20221 ggcggatcat agcttcgccg ccatactgct tcaatccggg caagcaacac ctgtggtgaa
    20281 aacggtttgg taatgtagtc gtctgcaccg gcattcagac cggtaataca gtcttcttcg
    20341 ctactgcggg ctgttagcat aatgattgga attttttcgg tttcaggctg tttttttagg
    20401 tatttaatta aatggatacc cgaacgcccc ggcatcatcc aatccagtaa aataagctcg
    20461 ggtttttcgc ccagtttatt gatagcactt tggtagtctt ccgcttcaat tacctgatat
    20521 tgtttgctga gatagagact tagcatttca cgaatagcac gttcgtcttc aacgatcaga
    20581 attttgttat tcattcttcc tctccgatgc ggtgtgacaa gcggtcgatt tatcgcagaa
    20641 atttgcaaat tttttactaa aaccgaccgc ttgcagatta ccccatttta cctttaatgt
    20701 aatcttccgt ctgccggaat tttggtttgt cgaaaatttg tttggtatca ccgaattcaa
    20761 tcagctcgcc tagatacata taagcggtat agtcagaaca gcgggctgct tgctgcatat
    20821 tgtgggtaac tatcgctacc gtgtagtcgt gcttgagttc gttgataagt tcttcgattt
    20881 tcatagtcga aatcgggtca agtgcggagc agggctcatc cagcagtaac acctcgggtt
    20941 tcactgcaat acctcgtgca atacagagcc gttgctgttg cccgccggag agactgtcac
    21001 cattttggtt taatttatgc tttacctcat tccataaagc cgctttggtt aatgcccact
    21061 ctacacgatc gtttagctcg gatttcggca atttttcaaa cagacgaata ccaaaagcaa
    21121 cattatcgta aatggacatc gggaatgggg tcggtttttg gaaaaccata ccgactttgg
    21181 ctcgaataat cgaaatatcc gtatcggagg tgagtagatt ctctccatca agattgattt
    21241 cccccgttgc acgttgattc gggtagagct caaacattcg gtttagggtt cgcagtaatg
    21301 ttgattttcc acaaccggaa ggaccgataa atgcggtcac tttatttttg gcaatacgca
    21361 tattgatatt tttcagagcg tggaaatcgc cataataaaa attgaggttg ttaatatgta
    21421 attttgtctc taaatccgtt ggtaacatat agagtcctta ttttgttttg ctgaaataca
    21481 gacgagtgat gatattaaga gaaagcacaa acagagtaat aagcattgca cctgcccaag
    21541 ccaatgtatt ccagtcagcg aacgggctgg ctgcgtattg ataaatcacc acagggaggt
    21601 ttgccattgg tgcattcata tctaaagagg caaactggtt ggataatgcg gtaaacaata
    21661 gcggtgcggt ttctccggaa atacgggcaa cagcgagcaa cacgccggta ataatgccgg
    21721 agcgggcggc acgataagag atcattgtaa ttaccctcca tcgtgggcag ccgagagccg
    21781 cagctgcttc acgcagatta ttaggcacaa gattgagcat actgtcggta gttcgaacaa
    21841 caatcggaat cactaatacc gccaatgcta atgagcctgc ccagcctgaa tagtgtttaa
    21901 cctgtgcgac ataaatcgca taaataaata agccgataat aatagaagga gcagagagca
    21961 acacatcatt caaaaaacgg gtgaattttg ccaatttggt ataacgcccg tattcgctca
    22021 agtaagtgcc ggctaaaata ccgattggtg ttgcaatcag tgttgcggta ctgacaatca
    22081 gtatagagcc gacaattgca ttacgtaatc ctcctgtttc atttggtgca ggagtcgggc
    22141 gggtaaacag ttcccaagac agtgcaggga tacctttttg gagtaaagta acaataatcc
    22201 agaccagcca aaataagcca aataatacag cggaataggc aagacttaac atcaagacat
    22261 tttttatttt tcggcaataa aagcgtcggt tttggtaatt tttcattctc gttttccttc
    22321 ctgtttaccc attcgtgcga ttaataaacg tgaagcggct aaaacaatcg aagtaataag
    22381 gaacagaatt agccctaggc tcatcaacgc tgatttttgt aatccgccgg cttcattaaa
    22441 ttcattggcg atagcagaag cgatggaggt tgaaggggaa aataccgaat caggtagcat
    22501 aaaggcatta ccgatcacaa aggtaactgc cattgtttcg cctaatgctc gacctaaccc
    22561 gagcataatt gcaccgatta ctccggtttt ggtgtaaggt agcacgactt tccaaataac
    22621 ttcccaagtg gttgagccta aaccgtaagc agactctttc agtaaaggcg gtacaacact
    22681 gaaaacatca cgcataattg aggcaataaa gggaataatc ataatcgcca ataccaagcc
    22741 ggcactgaat agcccgatcc cgaaaggagc tccgctaaaa agcataccga taaagggaag
    22801 ctgaccaaat gtatctatca aatacggttg cacatattct tgaaataagg gtacaaatac
    22861 aaacagcccc cacataccgt aaataataga gggaatagcc gctagcattt ctaccgctgt
    22921 gccgacaggg cgttttagcc attgcggtgc aagttcggtt aagaaagtcg caatgccgaa
    22981 cgagaccgga acggcaatga ctaatgcaat gccggcagaa atcaatgtgc cgataatcgg
    23041 cactaatgca ccgtatcggt cattcacagg gttccactcg ttttcccata ggaatgatac
    23101 cccgaatcgg cttaatgctt cccaactgcc aagcaataat gaaaccagca ccaccaccaa
    23161 caaggcaaaa accagcaaag caaagaaacg ggttgtgtga tgaaataacc cctctaccca
    23221 aggatggttt aagcatttgt tatcaatttt atgtttatgc atagtgacaa ctcttttgca
    23281 aaatgttgtg aaaaaacgac cgcttgttgt tagttaagct ccatacaagc ggtcgttttg
    23341 cattagtaga gcgaatttcc ttgtccatct ttaacttccg ttttccattg agtttggatt
    23401 ttctctacta cattatccgg tagaggcaca taatccagtt cggcagccgc atctttaccg
    23461 tgcttaaatg cccagtcgaa gaatttaaat acccctttag tttgctctgg tttgtcgcta
    23521 tgttgatgaa tcaagataaa actggcggcg gtaatcggcc aggctttttc tcccgcttcg
    23581 ttggttaaaa taacgcccat accggctgtt tggctccaat cggcgttagc agcggcagca
    23641 gcaaacgttt cacgggaagg ctgaacaaat ttaccatctg cattttgcat agaaacccaa
    23701 gccagagcat tttgtttagc ataggcatat tctacgtaac caatagaata cttgagctgt
    23761 ttcacataag ctgcgacacc ttcatttcct tttccgcctt gtccgaccgg ccatttcacc
    23821 gattttcctt cgccgacttt ctccttccat tcggcagaga ctttagaaag atagtttgtc
    23881 caaccgaaag ttgtgcctga accgtctgag cggcgtacga ctaaaattgt ttttgccggc
    23941 agggcgatat cttgattaag tgcgacaatt gccggatcgt tccattgtgt aattttgcct
    24001 aggaatatat cggcaagaac tttaccggac agtttcagtt cgcccgattt aagttcagga
    24061 atattgacaa ccggtacagt tcccccaatg atggcgggaa attgcaaaag tttgtgttct
    24121 gctaatgttt cgcctttcat cgggtcatca gaagcaccga aatcaattgt tttggcaata
    24181 atttgttgct gaccgccacc ggaaccgata gattggtaat tgattttatt accggtctct
    24241 ttttcatata aacctgccca tttggcgtag ataggataag ggaaagaagc accggctcct
    24301 gtgatggttt gagcatttgc tgcgacagaa agtgaaaaag aaatgcctaa aacggtaagt
    24361 agtttaaatc gtttggcaat catattgttc tccttaaagt tagcgtgagt taatttcacg
    24421 tgcttagtgt agaaaaagaa tatgacaaaa tgatgacaat ggtaaaaaaa tcagaaaaaa
    24481 cgaccgcttg tgtttcaggc tagactaaac aggctaaatc gtagaaaata gccctcgttt
    24541 ttttaatata aatatcgata aaaaatgacc acagaaatca attatgaagg cattgaacag
    24601 atgccgatta agcgttttac cgaagatgct tacctcaatt attcgatgta tgtaattatg
    24661 gaccgggcat tgccgtttat tggcgatggc ttgaaaccgg tgcaacgccg tattatttat
    24721 gcgatgtcgg aacttgggct aaacgcctcg gcaaagtata aaaaatcagc ccgtaccgtg
    24781 ggggatgtat taggtaaatt ccatccgcac ggtgatagtg cctgctatga agcaatggtg
    24841 ctaatggctc aaccgttctc ttaccgctat ccgttagtgg acggacaagg caactggggg
    24901 gctccggacg atccgaaatc cttcgctgca atgcgttata ccgaatcgaa actgtctaaa
    24961 attgcagaaa ttttattggg cgagttaggg caaggaacgg tggattatca gccaaatttt
    25021 gatggctcgt tggaagaacc gaaatatctg ccggctcgtt taccgcatat tttgttgaat
    25081 ggcacgatgg ggattgcggt gggaatggca accgatattc caccacacaa tattaatgaa
    25141 ttggctgatg ccagtgtgat gttgctggat aacccgaaag caacgcttga tgatgtacta
    25201 agcgtggtac aagggccgga ttacccgaca gaggctgaaa tcatcacacc aaaagcggaa
    25261 attgccagaa tgtatgagca ggggcgtggt tctattaaaa tgcgtgcggt gtggaaaaaa
    25321 gaagagggtg aaattgtgat ttcagccttg ccgcaccaag cctctccttc aaaaattatt
    25381 gagcaagtag caacccaaat gcggaataaa aaactaccga tggtggacga tattcgtgat
    25441 gaatctgacc acgagaaccc gattcgcatt gtgattgtgc cacgctctaa tcgcattgat
    25501 tttgatgcgt taatggatca cctgtttgcg acgaccgatt tggagaaaag ctaccgagtc
    25561 aatatgaata tgattggctt agacggcaag ccggcagtga agaatttgct gactattctc
    25621 aatgagtggc tgagttttag acgtaccact gtcactcgcc gcctgaatta ccgattagat
    25681 aaaattttaa accgcttgca tattttagac ggtttgatga ttgcatttct caatattgat
    25741 gaagtgattg agattattcg caatgaagat gagccgaaag ccgagttaat ggctcgtttt
    25801 aatctgaccg atgtgcaagc agaggcgatc ctgaacttac gtttacgcca cttagccaaa
    25861 ttggaagagc atcaattaca ggcggaaaaa tccgaactgg aaaaagaacg tgatgaattg
    25921 caactgattt taggctcgga acgccgtttg aacagcctga tcaagaaaga gattcaagcc
    25981 gatgccaaag catttgccag cccacgccgc tcaccattgg ttgaacgagc ggagtctaaa
    26041 gcgattgcgg aaagtgactt gactccaacc gaagatgtga ctgtgatttt gtctgaaaaa
    26101 ggctgggtac gttgtgcgaa aggacacgat attgatgttc aagccctaag ccatcgagcc
    26161 ggagatggtt atcttgccca cgcaaggggt agaagtaatc agcctgtggt atttttagac
    26221 agtacagggc gagcttatgc tctcgatcca acttcactgc cttcggcacg ctcgcaaggc
    26281 gaaccgctta caggcaaaat caccctaccg gaaggtgcag tagtgcagca agtgctaatg
    26341 gcaagtccag aaactaaagt gctaatggca tccgattcag gttacggctt tatttgcacc
    26401 tttgcagatt tagtttctcg taataaagcc ggcaaagcgg ttatttcttt aaccgaaaat
    26461 gcaaaagtac tgccgccgca attattggaa aatgatgaaa atttatcgct tgtagcgatg
    26521 agtaatgttg gcagaatgtt ggttttcccg gtgagtgaat tacctcaact ttccaaaggt
    26581 aaaggcaata aaatcatcaa tatttccgcc gcagcggcaa aatccggtga tgaatattta
    26641 gcccgtttat tagtcatcaa accaaccaac tcgctggtct ttgtgtccgg taaacgcaaa
    26701 atcacattaa aaccgagcga tgttgataac taccgtggcg aacgagcaag aaaaggctca
    26761 cagctagtga gagggcttag taccaattca acggtagaga ttgttgagta aagagaagaa
    26821 gcggttaggg ggtaaatcca acaattttgc cccaaataac cgcttgcaat ggagttattg
    26881 cttattcaaa taagcctttg tgaagcgaac gaaccacatc gtctgcttct gcatttttca
    26941 ctaaggtaca aacattattg ctgcttgctc catagctgat taggcgaatg ttgtaattct
    27001 caagagtgtg gaataatttt ttcgccacac cggactgagc gtgtaagttg ttgccgataa
    27061 tagcgactaa agcgaggtcg ctttctactt gcacattgca atatgcgtta agctcatcga
    27121 gtaattccgc tgataacata tctgcacctg aagaggctga gcccgtttta tctagggtca
    27181 atgcaacgct gacttcggaa gtggtaatca catcaaccga aattttgtgt ttcgctaaaa
    27241 tcgcaaatac attcgctaag aaaccttgtg cgtgtagcat tgaaaggctg gaaagcgtga
    27301 gtaaaatttg gttgcggcgt aatgcaatcg cacggaaagt cgggcgtggt tgtggatcac
    27361 gggttaccca agtgccgcct tgttccggtg ctttgcttga gccaacatat acagggatat
    27421 tgctacgcac tgccggtaat aaggttgctg ggtgtaatac tttcgctcca aatgttgcca
    27481 tttctgccgc ttcgttaaat gccattgtgt caatgcgttg agcgttcggt acaacacgag
    27541 gatcggtggt ataaatcccc ggtacgtctg tccaaatgag tacatcgtta gcatttaata
    27601 cttctgctaa caatgcagca gagtagtctg aaccgccacg tcctaaagta gtggttttgc
    27661 cttcatcatc acgaccaata aagccctgtg taatcacaat tttgcctgat gcgattaatg
    27721 gcttaatcac attgtctgat tgttgctggg ttttctcgtc atttggtgct gcttttccat
    27781 aatggctgtt tgttgccaca acgtcacgca catctaccca tactgccggg tgtccacgtt
    27841 caattaataa ttgcgtgaag attttggtgg acatcatttc cccatggctg attaactcat
    27901 cacttaaggc agctgaagta gcaaggctgg ctgattctgc taatgtttca atacgttcta
    27961 ataattcgtc gatttccgct ttaacggcat cttgatgttg taatttttca ataatattgt
    28021 attgaatttg gcggactgct gcgatgactt catcacggtg ttctttttca cagccgttag
    28081 caagttccac taagtaattg gttacgccag cagaggcaga aagtaccacc acacgagtat
    28141 ttgcatcagc aattacaata ttggcagaag aggtcattgc atcaaaattt gcaacacttg
    28201 taccaccaaa tttagccaca gaaagatgag acataataaa aatttcctta aaacaaaaat
    28261 agataaatat tgtgttaggg gatattcgga aaggaaattt gagtagatta tacggtttta
    28321 ttgttgaacc aattaatcag agcactccat ttaatatggt gaaataacct attaaatgac
    28381 agctgatagg attcaaccta ttcagccgat gttaattcca gtctagtgaa ttcacacctc
    28441 ggcaatcact cccatcactt aattctcaaa cggccctagt tgcctaaaat caagttgcct
    28501 gagtgttgca cctctcccgt aatggcaagc ccattactct ttttcaacag tattgaaaag
    28561 tgtgcataga aataccgttt tattaatgtt ttgtcaatga atatttaggt a
//
LOCUS       DANLZC010000012        28440 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.012, whole genome shotgun sequence.
ACCESSION   DANLZC010000012 DANLZC010000000
VERSION     DANLZC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28440
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.012"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            34..618
                     /locus_tag="PXE44_000432"
     CDS             34..618
                     /locus_tag="PXE44_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein HlpB"
                     /protein_id="HDL5587976.1"
                     /translation="MNKLTKISATALVALFLAACDKPAEKAPATEPTKTTEAAPTTTA
                     PAAEAAKPAETAVAAPSEAAKADYQKLVDWNTAQESTMATAQAELQKKLATQDPKQIQ
                     EGLSAFNKKVEETVKSLDAIEVSDAQIKTFKEKTKSVLTLSSEVISEQVKTISTPNDQ
                     AALQAVQQKAQSLIEAGNELQKLNVELQQRFSAK"
     gene            685..1341
                     /locus_tag="PXE44_000433"
     CDS             685..1341
                     /locus_tag="PXE44_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein Hlp"
                     /protein_id="HDL5587977.1"
                     /translation="MNTLAKISLSVLFTLFLTACEQPNSTKTQSSAESPVQVKEESKE
                     EVKPADTGAQDYKMLREWQDTQEKALNDAIKAATETLTDKQKADSTLMQETVNNALLA
                     QIDHIKISAETLNIQNNEVKALKDKTLEVLTLGAQMIVEGAKMEKNPTPEAHKAFGEL
                     QTKLNQLAEEGQQLENILRAKYDPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPAPQQ
                     "
     gene            complement(1422..2357)
                     /gene="ftsX"
                     /locus_tag="PXE44_000434"
     CDS             complement(1422..2357)
                     /gene="ftsX"
                     /locus_tag="PXE44_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDL5587978.1"
                     /translation="MIRSFSKLGAQTNYSLRAVWQDLMQRKLGTFLTVLVIAVSLTIP
                     TVSYLLWKNTHEAATQFYPEPQLTVYLHKNLAEHDVNTVVDRIRAFETDKIESFNYIS
                     RQQSLDEFRSWSGFGEALDILDENPLPAVVTLKPKANFNNADTMVELRNGLQQIKGVQ
                     EVRLDNGWLEKLTALTWLIARIAVVCTLLMLLAVFLVIGNSVRTDVANSKAAIEVQQI
                     LGATDHFIARPFLYTGMIYGLFGSLLAVLFSAVTISYFTGVVKYVTDMFTVQFELNGL
                     DFSEAFFLVVLCVFIGWLSAKIATMRNIFRIGSRG"
     gene            complement(2367..3020)
                     /gene="ftsE"
                     /locus_tag="PXE44_000435"
     CDS             complement(2367..3020)
                     /gene="ftsE"
                     /locus_tag="PXE44_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL5587979.1"
                     /translation="MIKFTNVNKVYKGGKPALQGIDFHLPAGGMAYITGHSGAGKSTL
                     LKLIMGIERANGGQILFNGHDITRLAHHELPFLRRQIGMVHQDYRLLTDRTILDNVAL
                     PLIILGMNQAIVEREARIALERVGLADKANYLPLHLSGGEQQRVDIARAIVHRPTLLL
                     ADEPTGNLDEKLSFEIFRLFEEFNQAGTTVLIATHDTNIISKRPKPCLVLEQGHLKF"
     gene            complement(3013..4509)
                     /gene="ftsY"
                     /locus_tag="PXE44_000436"
     CDS             complement(3013..4509)
                     /gene="ftsY"
                     /locus_tag="PXE44_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL5587980.1"
                     /translation="MSEKKKGFWSWLGFGKKEEAEQKEIQQEQESIQPEQPVIVEPET
                     EKSFSEKLDELEDKIEEKVDQLEDYFEEKEEKIEQFAEQVEDTIEQKVEDFKEFVEEK
                     VEEAKAFFDDVEDKIEQTFEEQKGEQAVENLQNIATEQLDLNEQEQVTYQVEEAVAEE
                     VAETELVNNVQDTVEESTIQDEYQEKPSQGGFFSRLIQGLIKTKQNIGSGFRNFFRGK
                     KIDDELFEELEEQLLVADLGMPTTQKIINNLTQHATKQQLKDADLLYQQLKVELGEVL
                     KPVAQPLVLEAKKPYVILMVGVNGVGKTTTIGKLARKFQQEGKSVMLAAGDTFRAAAV
                     EQLQVWGERNNIPVVAQSTGSDSASVIFDAMQSAAAKGIDVLIADTAGRLQNKNNLMD
                     ELKKIVRVMKKYDETAPHEIMLTLDAGTGQNAISQAKLFNEAVGLTGITLTKLDGTAK
                     GGVIFAIADQFNIPIRFIGVGEKIEDLRPFNAEEFIEALFSHEDEQND"
     gene            4588..5178
                     /gene="rsmD"
                     /locus_tag="PXE44_000437"
     CDS             4588..5178
                     /gene="rsmD"
                     /locus_tag="PXE44_000437"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL5587981.1"
                     /translation="MKKNRNSPQSVQQMGEVRVIAGLWRGRKLPVLNAEGLRPTTDRV
                     KETLFNWLMHDIAHSRCLDCFAGSGSLGIEALSRQAQAVVFLEKFANAANQLKKNLQA
                     LKSEQGSVIHTDTLAYLAQKNNGEPFNIVFIDPPFHQGFVPQVLNLLAENNWLAPNAI
                     LYVETEKNHPPLELPQGWQILKEKAAGMVVSRLIQL"
     gene            5262..5576
                     /locus_tag="PXE44_000438"
     CDS             5262..5576
                     /locus_tag="PXE44_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDL5587982.1"
                     /translation="MKKLTPDEAIDIAYDIFLEMAGENLDPADILLFNLQFEERGAVE
                     MVETSEDWDQEIGTLIDPDAFAEVWVGLVNEQDEMDDVFARFLISHDEENREYHVVWK
                     EK"
     gene            5589..6101
                     /gene="hpaC"
                     /locus_tag="PXE44_000439"
     CDS             5589..6101
                     /gene="hpaC"
                     /locus_tag="PXE44_000439"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HDL5587983.1"
                     /translation="MANIDTNQPIEASLKFREAMAHLSSAVSIVTTNGVAGKAGLTVS
                     SVCSVTDSPPTLLFCINKNSHVHDIIQQNGKVCVNVLSHEQEDLAKHFAAILESTMEE
                     RFGWDIWNNGFDDQPVLRSAISALQGNIVDTHAVGTHTIFVVEVSHIDVEPNHSLVYF
                     ARQFRSVNID"
     gene            6219..7091
                     /gene="tehB"
                     /locus_tag="PXE44_000440"
     CDS             6219..7091
                     /gene="tehB"
                     /locus_tag="PXE44_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HDL5587984.1"
                     /translation="MENLICYKQMPVWTKATLPQMFQEKHNTKVGTWAKLTILKGSLK
                     YYELTEEGEIIKEDIFDANHQRPMVEPQLWHKVEALSDDLECQLAFYCKPEDYFAKKY
                     NLTATHSEVLNAVNYTKGGKALDLGCGRGRNALYLNLLGFDVTAVDHNQESIDFLNYM
                     IEKEQAQNITTAVYDINEATIGSQNGEYDLIISTVVMMFLNRERIPAIIENMQKNTKV
                     GGYNLIVCAMSTDDYPCPMPFSFTFKEGELADYYQGWELVKYNEDLGHLHKTDENGNR
                     IQLRFATMLAKKIS"
     gene            7229..8266
                     /gene="purM"
                     /locus_tag="PXE44_000441"
     CDS             7229..8266
                     /gene="purM"
                     /locus_tag="PXE44_000441"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL5587985.1"
                     /translation="MSNTQLSYKDAGVDIHAGNELVERIKPDVKRTRRPEVMGGLGGF
                     GALCALPTKYKEPILVSGTDGVGTKLRLAIDLNKHDTIGQDLVAMCVNDLVVQGAEPL
                     FFLDYYATGKLEVDVATNVVKGIADGCVISGCALVGGETAEMPGMYHEGDYDLAGFCV
                     GVVEKSKIIDGSTVKTGDALIALASSGPHSNGYSLIRKVLEVSRSNPATDLLEDKPLS
                     EHLLAPTKIYVKSILKLVEEVDVHAIAHLTGGGFWENIPRVLPESAKAVIDESSWQWP
                     AIFSWLQEKGNISRYEMYRTFNCGVGMVIALPERDVEKALTLLKESGENAWLIGKIED
                     LGTGSEQVEIR"
     gene            8344..8622
                     /locus_tag="PXE44_000442"
     CDS             8344..8622
                     /locus_tag="PXE44_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL5587986.1"
                     /translation="MQLFWPIYFVLMNLAALYLMYSDKQKAIKKEWRVPEITLLSFCL
                     LGGAIGVYLGMKYFRHKTQHWYFHVAVVLSTFAWLIGLPYFYLALSSY"
     gene            8765..10192
                     /gene="aspA"
                     /locus_tag="PXE44_000443"
     CDS             8765..10192
                     /gene="aspA"
                     /locus_tag="PXE44_000443"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDL5587987.1"
                     /translation="MSDVRIEHDLLGEREVPNSAYWGIHTLRAIENFNISNVTISDVP
                     EFVRGMVMVKKATALANGELGAIPKKIAKAIVAACDEVLVKGRCLDQFPSDVYQGGAG
                     TSVNMNTNEVIANLALEILGEAKGKYDIVNPMDHVNASQSTNDAYPTGFRIAVYNSIT
                     KLINKITYLQTAFENKATEFSDVLKMGRTQLQDAVPMTLGQEFKAFAVLLDEEVRNLK
                     RTAELLLEVNLGATAIGTGLNTPEGYSTLAVKYLAEVTQLPCSLSENLIEATSDCGAY
                     VMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEV
                     VNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQAMFESIELLSNACINLRDKCIDG
                     ITANRDICQNYVFSSIGIVTYLNPFIGHHNGDIVGKICATTGKSVREVVLERGLLTEE
                     QLDDILSIENLMNPTYKAKRFTEEE"
     gene            complement(10267..12285)
                     /gene="rep"
                     /locus_tag="PXE44_000444"
     CDS             complement(10267..12285)
                     /gene="rep"
                     /locus_tag="PXE44_000444"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDL5587988.1"
                     /translation="MKLNSQQQQAVEYTKGACLVLAGAGSGKTRVIINKIAHLIAHCG
                     YSPKQIAAVTFTNKAAREMRERVDHSIGKENSKGLTISTFHTLGFEILKREYKLLGFK
                     SGMTLFDEQDQIALLKHLLPENVTEDKDLLKALIATISNWKNDLLSPERVLTRVRDER
                     ERVFSHFYQLYQNQLKAYNALDFDDLIMLPVLLFRQFPEAKSRWQQKIRYLLVDEYQD
                     TNTSQYELIKLIVGNEPNFTVVGDDDQSIYSWRGAKPENMQRLREDFDLQVIKLEQNY
                     RSSQRILHCANILIDNNDHLFSKRLFSELGQGEKLQVIEAKNEEHEAERIVGELIAHR
                     FSKKTKYKDYAILYRGNHQSRLLEKLLMQNRIPYKISGGTSFFARAEIKDMMAYLRLV
                     VNQDDDAAFLRIVNTPKREIGAATLEKLGILANEKQVSLFEAIFDFELIQRLTPKPYQ
                     ALQDFARWIVEIADQADRSDPIEAVKDLLAKIQYEAYLYEMAANPKVAEMQSRNVQTL
                     FEWVQAMLEGDEIEPPMNLVQVVNRLTLRDMLERGEEDDEADQVQLMTLHASKGLEFP
                     HVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQYGEIIKP
                     EPSRFLLELPQDDLEWESEKPRMTEQEKEAKSSANIANLMAMLKANKK"
     gene            12533..13648
                     /gene="potA"
                     /locus_tag="PXE44_000445"
     CDS             12533..13648
                     /gene="potA"
                     /locus_tag="PXE44_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDL5587989.1"
                     /translation="MEKVEQKAIIELKNVTKSYGSKTILKNLNLTINDGEFLTILGPS
                     GCGKTTALRLIAGFEDLTEGSIILDGQDVSNVPAEQRPVNTVFQSYALFPHMTIFENV
                     AFGLRMQKVPNAEIEPRVMEALRMVRLEDRAQQKPAQLSGGQQQRIAIARAVVNKPKV
                     LLLDESLSALDYKLRKEMQNELKALQRQLGITFIFVTHDQEEALTMSDRIIVMNAGKI
                     AQDGTPREIYEEPSNLFVARFIGEINVFDATVIERVDATNVKANVEGRICNIKVEDLE
                     VQPNQKLKVLLRPEDIVIEELDENESSKAIIGHIIDRNYKGMTLESSVRLDHNGMNVL
                     VSEFFNEDDPNIDHEVGQKVALTWYEGWEVVLNDEGE"
     gene            13635..14498
                     /gene="potB"
                     /locus_tag="PXE44_000446"
     CDS             13635..14498
                     /gene="potB"
                     /locus_tag="PXE44_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDL5587990.1"
                     /translation="MKANNKFQNGAVATIFGWLLLFVLVPNVLVLIISFLTENRQSQY
                     FVDFAFSFDAYKSLFNDTYAAVLWNSLYMAGIATFFCLIIGYPFAFFIAKMPERIRPF
                     LLFLVVLPFWTNSLIRIYGIKIFLGVKGFLNESLMALGIISEPMRLLNSEIAIIIGLV
                     YILLPFMILPLYSSIEKIDNRLLEAAKDLGANAFQRFIKVTIPLTMPGIVAGCLLVLL
                     PAMGMFYVADLLGGGKTPLVGNIIKSLFLNTNQFALGSAVSIALTILMALMLYVYYRA
                     NKLLNKKVELE"
     gene            14498..15265
                     /gene="potC"
                     /locus_tag="PXE44_000447"
     CDS             14498..15265
                     /gene="potC"
                     /locus_tag="PXE44_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDL5587991.1"
                     /translation="MKKMFRNLFMLVVFAYLYIPIIILVINSFNEDRFGLNWKGFTWN
                     WYERLFSNDTLVQATLNSLTIAFFAATFSTILGALTSIALYRYRFRGKKLVGGMLFVV
                     MMSPDIVMAVSMLVLFMILGIKLGFISLLIAHITFCLPYVVIAISSRLSDFDGKMLEA
                     AKDLGASEVTILRKIILPLALPSIISGWLLSFTISLDDVIVSSFVTSPSYEVLPLKIF
                     SLVKTGVTPEVNALATIMIIFSLTLVVLSQLVVKKKV"
     gene            15466..16389
                     /gene="oppB"
                     /locus_tag="PXE44_000448"
     CDS             15466..16389
                     /gene="oppB"
                     /locus_tag="PXE44_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDL5587992.1"
                     /translation="MLKFIFKRLLEAIPTLLILITFSFFLMRLAPGSPFTSEKAYPPK
                     VMANIEAKYHLNEPLYKQYFLYMENLSKGDFGPSFKYKDQSVNNLIASAFPVSIKLGI
                     TAFIFAVLIGLTAGTIAALKQNSKWDYLVMGFSMTGIIMPSFVFAPLLVLMFAIYLKW
                     LPAGGWNGGQLYYMVLPVLSLTISYVAGIARITRGSMIEVLHSNFIRTAKAKGLPMRK
                     IIFKHALRPALLPVITYLGPAFVGIITGSMVIESVFGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGTLTILFNAIVDILYAVIDPKIRYS"
     gene            16404..17303
                     /gene="oppC"
                     /locus_tag="PXE44_000449"
     CDS             16404..17303
                     /gene="oppC"
                     /locus_tag="PXE44_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDL5587993.1"
                     /translation="MQNSSKNQPLVEVTSTIQGRSLWQDARRRFFRNKAAVASLIILC
                     CIILFITFAPMLMPFSYEDTDWNMMGIAPDFESQHYFGTDSSGRDLLVRVAIGGRISL
                     MVGIAGALIAVLIGTIYGAISGYFGGKVDMVMMRFLEILSSFPFMFFVILLVTFFGQN
                     IFLIFIAIGMVAWLNLARIVRGQTLSLKNKEFVEAAIVCGVSRRQIIWRHIVPNVLGL
                     VVVYASLEVPSLILFESFLSFLGLGTQEPMSSWGALLSDGAAQMETSPWLLAFPAFFL
                     CLTLFCFNFIGDGLRDALDPKDK"
     gene            17313..18908
                     /locus_tag="PXE44_000450"
     CDS             17313..18908
                     /locus_tag="PXE44_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5587994.1"
                     /translation="MELLAVNNLDVYLKTDEELVHAVRNVSFSIEKGQTLAIVGESGS
                     GKSVTSMAIMQLLPKNIVSFGERSSIIFEEQNLLTLSEKAMQTVRGDRIGMIFQEPMT
                     SLNPYMPIGKQVAETVQTHNPQISNAEAEKMVLETLQKVKIPDAEKKMICYPHEFSGG
                     QLQRIMIAMAIINKPDLLIADEPTTALDVTTQAEILDLMHDLQRDMGMAIILISHDLR
                     LVHKYSDLVCVMQNGEIIERGETETVFTNPQHPYTVELLTPIPTNLKGELPPNAPTLL
                     QGEHIKVDYILKRSLFGKPKKVFNALKDISLHLKTGETLGIVGESGSGKSTLGRAIMQ
                     ILDYRGNITFAGKTLANLNKAERQALKKDMQMVFQDPFNSLSPRLTVGEIIAEGLTVH
                     CPQMSKTERREKVMKMLEEVNLNPAMINRYPHEFSGGQRQRIAIARAIILEPKFVLLD
                     EPTSALDRSTQITVIELLNRLQKKYGLSYIFISHDLAVIKALSDRVIVMSQGEIVESG
                     DVTQIFENPQQEYTKRLILASNL"
     gene            19088..19483
                     /locus_tag="PXE44_000451"
     CDS             19088..19483
                     /locus_tag="PXE44_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5587995.1"
                     /translation="MKKLMIFATTAMIVSNLAHAAGEQSDAREAHESTVKTSTVKYSC
                     QNGKKLSVKYGFNKQGIPTYAEAKLSGKKRFMPINLYTTDATGTNFGDENNFSLYGDP
                     MEFTNHRKASVNIQSPASEILYKGCTPQK"
     gene            complement(19693..21852)
                     /locus_tag="PXE44_000452"
     CDS             complement(19693..21852)
                     /locus_tag="PXE44_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL5587996.1"
                     /translation="MQIKQTVLAIAITPFISQLSLAQETKISSIELDTIEVNEQQENL
                     LKQQAVMSNEDLVRYSTDMGVPQTGRFGPQGFNIRGVEGNQVAVTVDGISQAASNNYA
                     TFSRYSYYNKSRPEIDTELMSDVTIEKGSSTKVGNGALGGSVNYKTKTVSDILMKGRQ
                     FGGMLKYTYNGQNKENIETAGIAADGQYADALVMYSHRQGHETQTTSKGPDIMGSGRG
                     LPNPYKNNQYSYLAKLGLKWNDENRLELKTSKQRSKNAGQELSYSTVTLRDFLDVQEI
                     ETYGVTYLYTPKNSILENIKVAVTKQDTEISGINDQITIARGTKTDNTKRAFNTKNVE
                     GSLGVTFKPVDLGNTEHKLSTELGYGVSDFSFDMQTYTAGITPTDQNMQSPAKTKSFH
                     ILMGDSILLTQNLSAHLNARYEQSKLHSDDNSLSDKTFNNWAGDIGLNYQLSPVWQIG
                     YKLSRGFRTPTASEMFFNREVQGRGASQTFLANTNLKPETSLGNEISLVGNGSLGSLS
                     VAAYHTHYRNLIDLATIGNNIYQSQNVNRAKIKGVDINGTLDLNTAWNAIPQGFELNG
                     GFGYARGKRNDGTDLLTVQPLKALFGIGYRAVNNDWAIHLNGTYTQGKKAKYAQQYAI
                     LANTGYLSYDKSLTTYPYLSNSATVFDLVAHKKFGERAIVKVGVYNLFNKKYWTWDSL
                     RTIGITSPGVANSITGEGLNRYLAPERYVMASVEFKF"
     gene            21959..22825
                     /locus_tag="PXE44_000453"
     CDS             21959..22825
                     /locus_tag="PXE44_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5587997.1"
                     /translation="MEYKMRTLLFSFIALSMCLNTTKAAEKIVSTAGYASEIVAALGK
                     ADKLVGVDTTSVKPQTIMEKKPKIGYRRQLSAEGILSLTPDLIILAPDAGPNAVIEQI
                     KASGIPLLHLGEKPSLAGIREDIQQIAKAIGAESEAKQLTETILADEKKLADIRSQHS
                     TDKEVLVLIDTASQGIFGLGKKSVGDHFLTLLNLNNSFNAEGNKPLANEALAASNADV
                     ILLASRNQAKESATISKLAESSPQYAQLTNTQAGKKGCVFAINILDALGFGPYTAQYA
                     HQILTTIQPCLK"
     gene            22836..23822
                     /locus_tag="PXE44_000454"
     CDS             22836..23822
                     /locus_tag="PXE44_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5587998.1"
                     /translation="MKLHLTLIFTLIISLVICTHLGAMSLPLSAWHCNNAECESAAYI
                     LWQIRLPRLLGALFIGAALAISGATMQGLFRNPLVDPGIIGVSSGAALAAALYIVVIQ
                     PLLPESISYYALPIAAFLGGWLVTFMLYLLSQRNGQLHIAIMLLIGIALAAFSGALTG
                     ILIYVADDNQLRSLTFWSMGSLAGMNWQMVAMMAAVVFICSPLIWREHRALNALTLGE
                     EDAQYIGFDIAKVKRRLVFYVALLTGTSVACVGAIGFVGLVIPHLMRLLGGSNHRHLL
                     LNSLVCGAILLMIADTLARTLAAPAEIPIGIFTALFGAPFLAVMVWKSGKHI"
     gene            23822..24628
                     /locus_tag="PXE44_000455"
     CDS             23822..24628
                     /locus_tag="PXE44_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5587999.1"
                     /translation="MSNLLTCQNLSIVHQQKKLLEIQEIAFEQGKFTAIIGPNGAGKT
                     TLLKALSGNNAQTNSAVFYQHQRLTDLNGYALAQKRAMLSQQSQIAFPLKVEELVQLG
                     REPYRQSEFAKFNEKITAWAMETMSLTALKERSSIQLSGGEQHRAHIARVLAQLVPEP
                     NADLSGSWLLLDEPTNHLDIHHQYQLFSLLQKLKQQGLTIIAILHDPALAINQADHIL
                     MLKNGEKFAEMSAKAIAESCLLDELYEMKMGCRYCPHSGQYYLCPALAES"
     gene            complement(24649..25287)
                     /gene="purN"
                     /locus_tag="PXE44_000456"
     CDS             complement(24649..25287)
                     /gene="purN"
                     /locus_tag="PXE44_000456"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL5588000.1"
                     /translation="MKKFVVLISGNGSNLQAMIDAQKSADTSGQICGVICNKADAYGL
                     IRAKQAGIPTFVFSRKDYQSNVEMDLAIAEQIEQLGAELIVLAGYMKILTPEFTQHFA
                     GKILNIHPSLLPKYPGLNTYQRAIEAGESEHGTTIHFVNEEVDAGAVVLQAKVPIYPE
                     DEIEDVMARVVEQEHRYYPLVIEWFCSGRLVSQHGKAYLDGKLLPACGYAED"
     gene            complement(25394..27551)
                     /locus_tag="PXE44_000457"
                     /pseudo
     CDS             complement(25394..27551)
                     /locus_tag="PXE44_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(27605..28402)
                     /locus_tag="PXE44_000458"
     CDS             complement(27605..28402)
                     /locus_tag="PXE44_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HDL5588001.1"
                     /translation="MAFFLLGCSVSTQSLNEQNVDSIGQIIDLNTNQTISFTELLSRL
                     SSQDYVLVGEEHTQLIHHQIELYLFNQLTKKAKLHLIALEMLNVDQQPAIDRIQFNRP
                     VNLSATDLREAIQWQKWDWKMYQDLVVESLGSNSRVIATNLTDKEVEILLNGAEPLKG
                     SVSTSSEVKQKIAQLLLSMNHGMPYPMENMVAVQQFRDRRMAEKLVKNALSTSLLIAG
                     NHHVRKDLGVPLHIAEYDRTKKVAVLMLKTEREEITSSQADYLWVTQ"
BASE COUNT         8440 a         5919 c         5484 g         8597 t
ORIGIN      
        1 gcttttattt ttatattctt gaggaatttt ttaatgaaca aattaacaaa aatcagtgca
       61 accgctttag tcgcattatt cttagcagct tgtgataaac cagcagaaaa agcaccggca
      121 acagaaccta caaaaacaac tgaagctgca ccaactacaa cagctcctgc tgcagaagca
      181 gccaaacctg ctgaaacagc tgtagccgct ccaagcgaag ctgcaaaagc agattaccaa
      241 aaattagttg attggaatac ggcacaagaa agcacaatgg caacagcaca agcagaacta
      301 caaaagaaat tagctacaca agatcctaaa caaattcaag aaggattaag tgcatttaat
      361 aaaaaagtag aagaaactgt aaaaagctta gatgcgattg aagtatctga tgcacaaatc
      421 aaaacattta aagaaaaaac aaaatcagtt ttaacacttt caagtgaagt gatttctgaa
      481 caagtaaaaa cgatctctac accaaatgac caagcagcat tacaagccgt acaacaaaaa
      541 gctcagtctt taattgaagc tggtaatgag ttacaaaaat taaacgtaga attacagcaa
      601 agattctcag ctaaataatt taccattcaa tgaaaagcta gagtattttg ctctagcttt
      661 tttttgcaat tactaaggat tactatgaac acgctcgcta aaattagcct atctgtatta
      721 tttacccttt ttttaaccgc ttgcgaacaa cctaattcta ccaaaacaca gagctcagca
      781 gaatcgccag ttcaagtgaa agaagagtca aaagaagaag ttaaacctgc tgataccggc
      841 gctcaagatt ataaaatgtt acgggaatgg caagacactc aagaaaaagc cttaaatgat
      901 gcaattaaag cggcaacaga aacacttact gacaaacaaa aagccgattc taccttaatg
      961 caagagacag ttaataacgc attattagct caaattgatc atattaagat aagtgctgaa
     1021 accctgaata ttcaaaataa tgaagtcaaa gcactaaaag acaaaacctt agaagtcctg
     1081 actttagggg ctcagatgat cgtagaaggt gctaaaatgg agaaaaatcc tacaccggaa
     1141 gcacataaag catttggcga gttacaaaca aaattgaatc aattagctga agaagggcaa
     1201 cagttagaaa atatattaag agctaaatat gatccagctc cagctccagc tccagctcca
     1261 gctccagctc cagctccagc tccagctcca gctccagctc cagctccagc tccagctcca
     1321 gctccagctc cacagcaata atatattgct tatagtaaaa aaccaagttc ctaggaactg
     1381 gtttttatct gcaattattt ctctaaaaac aaccgcttgt attagcctct actaccaata
     1441 cggaaaatat ttcgcattgt cgcaattttt gccgacagcc agccaataaa cacacaaagc
     1501 accactaaga agaaggcttc gctgaaatct aatccgttca gttcaaattg tacggtaaac
     1561 atatcagtca catacttcac tacaccggtg aaatagctaa tcgttaccgc actaaacagc
     1621 accgctaaca ggctgccaaa caagccgtaa atcatacctg tgtataaaaa cggacgggca
     1681 ataaagtgat cagttgcccc taaaatttgt tggacttcaa tcgctgcttt gctattggca
     1741 acatcggttc gcacactgtt tccaatcact aaaaaaactg ccaacagcat taataaggta
     1801 cacaccaccg caatacgagc aatcagccaa gttagagcag ttaatttttc cagccagccg
     1861 ttatctaaac gaacctcctg caccccttta atttgttgca aaccgttgcg tagctccacc
     1921 atcgtatcag cattattaaa atttgctttc ggttttaagg taacgaccgc aggcaacggg
     1981 ttttcatcta aaatatctaa cgcttcacca aagcctgacc aagaacgaaa ttcgtctaaa
     2041 ctttgttggc gagaaatata gttaaatgac tcaattttat ccgtctcaaa agcacgaatg
     2101 cgatcaacaa cggtattcac atcgtgttct gccaaatttt tgtgcagata aacggttaat
     2161 tgtggctcag ggtaaaattg tgttgctgcc tcgtgggtat tcttccaaag taaataactc
     2221 acggttggaa tcgtaagtga gaccgcaatc accagcaccg tcagaaaagt gccgagcttg
     2281 cgttgcatta aatcttgcca aaccgctctt aaagagtagt tggtttgagc acctaattta
     2341 ctaaatgaac gaatcataat atttccttaa aatttaagat gcccttgctc cagcactaaa
     2401 cacggctttg gacgctttga aataatattg gtatcgtggg tggcaatcag caccgttgtg
     2461 ccggcttgat taaattcctc aaacaaacgg aaaatttcaa acgagagctt ttcatctaag
     2521 tttccggttg gctcatcggc taataacagc gttgggcgat gaacaatggc cctggcaata
     2581 tcgacacgtt gttgctcacc gccggaaagg tgcaacggca gatagttcgc tttatctgcc
     2641 aagccaacac gctcaagggc aatgcgggct tcacgctcca caatcgcttg gttcattccc
     2701 aaaataatca atggtaatgc gacattatcc aaaatggttc ggtcggttaa taagcgataa
     2761 tcttgatgca ccatcccgat ttggcggcgt aaaaacggca attcatgatg agcaagtcgg
     2821 gtaatatcgt gtccgttaaa caaaatttgc ccgccattgg cacgctcaat ccccataatt
     2881 aattttaata gcgtgctttt ccccgcaccg gaatgcccgg taatatatgc cattccaccg
     2941 gcaggtaggt ggaaatcaat gccctgtaag gctggctttc cgcccttata aaccttgttc
     3001 acattagtga atttaatcat tctgttcgtc ctcgtggcta aataaggctt caataaattc
     3061 ttctgcatta aacgggcgta gatcttcaat tttctcgccc accccaataa agcggatcgg
     3121 aatattgaat tgatcggcaa tcgcaaaaat cacgccacct tttgccgtac cgtctaattt
     3181 agttaaggtg ataccggtta aacccactgc ctcattaaac aatttcgctt ggctgatcgc
     3241 attttgccca gtgccggcat caagcgtaag cataatttcg tgcggtgcgg tttcatcata
     3301 ttttttcatc acccgcacaa ttttcttcaa ctcgtccatc aggttatttt tgttttgcaa
     3361 acgccctgca gtatcagcga ttagcacgtc aatccctttt gccgcagcgg attgcatcgc
     3421 atcaaaaatc accgaggcag aatccgaacc ggtgctttgt gccaccaccg gaatgttatt
     3481 tcgctctccc caaacctgta gctgttcaac tgccgccgct cggaacgtgt ctcctgctgc
     3541 taacattacc gatttgcctt cttgttggaa tttacgggca agtttgccaa tggtagtggt
     3601 tttccccacg ccattcacac caaccattaa aatgacatac ggttttttcg cttccaacac
     3661 taacggttga gccaccggtt ttagcacttc acccagttcc actttcaact gttgatacaa
     3721 caaatctgca tcttttaatt gttgcttggt agcatgttgc gttaaattgt tgatgatttt
     3781 ttgtgtggtc ggcataccta aatccgccac aagcaactgc tcttccagct cttcaaataa
     3841 ctcatcatca attttcttgc cacggaagaa attacggaag cccgaaccaa tattctgctt
     3901 ggttttaatc aagccttgaa ttaaacgact gaagaaaccg ccttgactcg gtttttcttg
     3961 atattcatct tggatagtag attcttcaac tgtgtcttga acattattta ctaattcagt
     4021 ttcagcaact tcttcagcca cagcctcttc gacttgataa gtaacttgct cttgttcgtt
     4081 gagatctaac tgctcagttg caatattttg aagattttcg accgcttgtt cgcctttttg
     4141 ttcttcaaat gtttgttcga ttttatcttc aacatcatca aagaacgctt tcgcctcttc
     4201 aactttttct tcaacaaact ctttgaaatc ttcgactttt tgttcaatgg tatcttctac
     4261 ttgctcagca aattgctcaa ttttttcttc tttttcctca aaatagtctt ctaactgatc
     4321 taccttttct tcaattttat cttccagctc atctaatttt tcagaaaacg acttctcagt
     4381 ttccggttca acaataacag gctgttccgg ttgaatactc tcttgttctt gttgaatctc
     4441 tttctgttca gcttcttctt tcttaccaaa tccaagccaa gaccaaaacc cttttttctt
     4501 ctctgacata tttttctctc atttctcgta aaaatagact aaacttacat agtctatcaa
     4561 aaaatccctt attttccttt agtgagtatg aaaaaaaatc gaaattcccc ccaatctgtt
     4621 cagcaaatgg gcgaagtacg ggttatcgcc ggactgtggc gaggcagaaa attacctgta
     4681 ctaaatgccg aggggcttcg cccgacaacc gatagagtaa aagaaacgct gtttaactgg
     4741 ttaatgcacg acattgctca ctcacgctgt cttgattgct ttgcaggcag tggctctctg
     4801 ggcattgaag ccctttcccg ccaagcacaa gcggtcgttt ttttggaaaa atttgcaaat
     4861 gctgccaacc agttaaagaa aaatctccaa gcattaaaat ccgaacaagg tagcgttatt
     4921 cacactgaca ccctcgccta tttagcacaa aaaaataacg gtgagccatt taacatcgta
     4981 tttatcgatc cgccttttca tcaagggttt gtacctcaag tgctaaactt gctggcagaa
     5041 aataactggc tcgccccgaa tgcgattttg tatgtggaaa ccgagaaaaa tcacccgcca
     5101 ttagagctac cgcaaggctg gcaaatttta aaggaaaaag cagccggaat ggtggtaagc
     5161 cgtctgattc agctataatc tttttgtttt ttataactaa aaaatataaa ataaaactta
     5221 tctatctaac attttattaa cactcaaatt agaataacga aatgaaaaaa ttaacccctg
     5281 atgaagccat tgatattgcc tacgatattt tcttagaaat ggcaggcgaa aatttagatc
     5341 ctgccgatat tttactgttc aacctccaat ttgaagagcg tggtgcagtg gaaatggtcg
     5401 aaacttctga agactgggat caagaaatcg ggactttaat tgaccctgat gcctttgccg
     5461 aagtttgggt tggcttagtc aatgaacaag atgaaatgga cgatgtattc gcccgtttct
     5521 taatttccca cgatgaagaa aaccgtgaat atcacgttgt ttggaaagaa aaataaaaag
     5581 gagaaaaaat ggctaatatc gacactaatc aaccaataga agcctcgctt aaatttcgtg
     5641 aagcaatggc tcacttatct tctgcggtga gcattgtgac aaccaatggc gtagccggca
     5701 aagcagggct gacggtttcg tctgtttgtt ccgttaccga ttccccacca acgctcttat
     5761 tttgcattaa taaaaacagc cacgttcacg atattatcca acaaaacggc aaagtatgcg
     5821 ttaatgtgtt aagccacgaa caagaagact tagcaaaaca ttttgccgcc attttagaaa
     5881 gtacaatgga agaacgcttt ggttgggata tttggaacaa cggctttgac gatcagcctg
     5941 tgttacgcag tgctatttct gctttacaag gcaatattgt tgatacgcac gcagtgggta
     6001 cgcatactat ttttgtggta gaagtgagcc atattgacgt agagccgaac cacagtttag
     6061 tttattttgc ccgccaattt agaagcgtga acattgacta aaatatattt aacagaaatt
     6121 tgatcaaatc cgactttttt agtcggattt tttatttcct gtttttaaaa cagtgctaaa
     6181 attttgccgt ttatgtttga ctgacaaaag gaaacaaaat ggaaaattta atttgctaca
     6241 aacaaatgcc ggtgtggaca aaagcaacct taccacaaat gttccaagaa aagcacaaca
     6301 ccaaagtcgg aacttgggca aaactcacca ttctcaaagg ttctctcaaa tattatgaat
     6361 taaccgaaga gggagaaatt atcaaggaag atatttttga tgcaaaccac caacgcccaa
     6421 tggttgagcc tcaactttgg cataaggttg aagctctttc tgacgattta gaatgccagt
     6481 tggctttcta ttgcaagccg gaagattatt ttgctaaaaa atataatctg accgctaccc
     6541 attcagaagt gctaaatgcc gtcaattata ccaaaggtgg caaagcgtta gatttaggtt
     6601 gcgggcgagg acgcaatgca ctttatctga atttactggg ttttgatgtc actgctgttg
     6661 atcataatca agaaagcatt gattttctca actatatgat cgaaaaagaa caagctcaaa
     6721 acattactac cgctgtttat gatattaacg aggcaactat cggctcgcaa aacggtgaat
     6781 atgacctgat tatttccact gttgtaatga tgttcttaaa ccgtgaacgc attcctgcca
     6841 ttattgagaa tatgcagaaa aacactaaag tcggcggtta taacttaatc gtatgtgcaa
     6901 tgagtaccga tgactaccct tgcccgatgc cgttctcctt taccttcaaa gaaggcgagc
     6961 tcgccgatta ttatcaaggt tgggaattag tcaaatataa cgaagattta ggacatttac
     7021 acaaaactga tgaaaacggc aaccgcattc aactacgttt tgcgacaatg ttggcgaaaa
     7081 aaatcagcta aacttaaccg cttgcagggg gaattagctt ccccctaatt catcaaatcc
     7141 attctattta ttttcacttt ttcattccgc aaacgtttgc ttgtgctata atggcttcgt
     7201 tttttagtca tttactgttg aggttaaagt gagcaacaca caactaagtt acaaagatgc
     7261 cggcgttgat attcacgcag gaaacgagtt agtcgaacga atcaagcctg atgttaaacg
     7321 cacccgtcgc cctgaagtaa tgggcggatt aggtggtttt ggtgctttat gtgctttacc
     7381 gacaaaatat aaagagccga ttttagtttc cggtaccgat ggcgtcggca caaaattacg
     7441 cttagccatt gacttaaaca aacacgatac tatcgggcaa gatttggtag cgatgtgtgt
     7501 gaatgacctt gtcgtgcaag gtgctgaacc gcttttcttc ttagattatt acgccaccgg
     7561 taaattagag gttgatgtag ccaccaatgt cgtcaaaggc attgcagacg gttgcgtgat
     7621 ttcaggttgt gctttagtgg gaggtgaaac ggctgaaatg ccgggaatgt accacgaagg
     7681 cgattacgat ttagccggat tctgtgtcgg tgtggtagaa aaatctaaaa tcattgacgg
     7741 ttcaacggtg aaaaccggtg atgccttaat tgcattagcg tccagtggcc cgcattccaa
     7801 cggctattcg ttaattcgta aagtattgga agtcagccga tccaatcctg caaccgattt
     7861 attggaagac aaacccttaa gcgaacactt attagcccca actaaaattt atgtaaaatc
     7921 aatcctaaaa ttagttgaag aagttgatgt tcacgctatc gctcacttaa ccggtggcgg
     7981 tttctgggaa aatattccac gcgtattgcc ggaatctgcc aaagcggtga ttgatgaatc
     8041 gagctggcaa tggccggcga tatttagctg gttacaggaa aaaggcaata tcagccgtta
     8101 tgaaatgtac cgtactttca actgcggtgt cggaatggtg atagccttac ctgagagaga
     8161 tgtcgaaaaa gcccttactc ttcttaaaga gagtggtgaa aatgcgtggc ttatcggtaa
     8221 aattgaagac ttaggcaccg gtagcgagca agttgaaatc cgctaatttg caaaataaat
     8281 gctaaaattg accgcttgta gtaactgatc tgcaaacggt taaattttta ttgaggaatg
     8341 caaatgcaac tattttggcc tatctacttt gtcttaatga acctagctgc cttatattta
     8401 atgtatagtg acaaacaaaa agcgatcaaa aaagaatggc gagtgccaga aattactctc
     8461 ctctcttttt gcctcttagg tggggctatc ggtgtttacc taggtatgaa atactttcgc
     8521 cataaaaccc aacattggta tttccacgtc gctgttgtgc tttctacttt tgcttggctg
     8581 attggcttgc cttactttta tttagcttta tcatcatatt aatttatcat tacttttttt
     8641 aatccgaaag cccaatttat atcctagtcc gctcacaaaa aatttgattt agatcgcatt
     8701 atttgtctat taaaatcgtt attatctacc aaaatcgatt gtcatttatt ttaaggagat
     8761 gaatatgtca gatgtgcgta ttgaacacga tttacttggt gaaagagaag tacctaatag
     8821 tgcttattgg ggcattcata cattaagagc gattgagaat tttaatatct caaatgtcac
     8881 catttctgat gtacctgaat ttgtgcgtgg catggtaatg gtaaaaaaag ctactgcgtt
     8941 ggcaaatggt gaattgggtg caatcccgaa aaaaattgcc aaagctattg tggcagcctg
     9001 tgatgaagta ttagtgaaag gacgctgtct cgatcaattc ccttcagatg tttatcaagg
     9061 tggagccggc acatcggtga atatgaatac caatgaagtg attgccaacc ttgcattaga
     9121 aattttgggc gaggcaaaag gtaaatatga tattgtcaat ccaatggatc acgttaatgc
     9181 aagccaatcc accaatgatg cctatccaac cggtttccgc atcgcggttt ataacagtat
     9241 cactaaatta atcaataaaa tcacttacct acaaactgca tttgaaaata aagccactga
     9301 gttttccgat gtactaaaaa tgggaagaac gcagttacaa gatgccgtgc caatgacgct
     9361 cggtcaggaa tttaaagctt ttgccgtatt attagatgaa gaggttcgta accttaaacg
     9421 gacggcagaa ttgcttcttg aagtgaattt aggcgcaacc gctatcggta ctggtctgaa
     9481 tacgccagag ggttactcta ctcttgcagt taaatattta gccgaagtca cgcaactgcc
     9541 ttgctcactg tctgaaaatc tcattgaggc aacatctgac tgtggtgctt atgtaatggt
     9601 tcacggtgca ttaaaacgca ccgccgtcaa actttctaaa gtgtgtaatg acttacgatt
     9661 actttcatca ggcccacgtg ccggcttaaa tgaaattaat cttccggaat tacaagccgg
     9721 ctcatctatt atgccggcaa aagtaaaccc tgttgtacct gaagtcgtaa accaagtgtg
     9781 ctttaaagtg attggtaacg acaccaccgt tacttttgca gcagaggccg gtcaattaca
     9841 gcttaacgta atggagccgg ttatcgggca agcaatgttt gaatctatcg agctgctttc
     9901 caatgcatgt attaatctac gtgataaatg tattgacggt attaccgcta accgtgacat
     9961 ctgccaaaac tatgtgttca gctctattgg tattgtgacc tacctcaatc catttatcgg
    10021 ccaccataat ggtgatattg tcggcaaaat ttgtgcaacc accggcaaaa gtgtacgaga
    10081 agttgtgctt gagcgaggct tactcaccga agaacaactc gatgatattc tctcgattga
    10141 gaacttaatg aatccgactt ataaagccaa acgctttacc gaagaggaat aatcattgtt
    10201 tacaagcggt ctgattctgg caaaaaattg caaattttta ccaaaatcag accgcttgtt
    10261 atcccattat ttcttatttg ctttcagcat cgccattaaa tttgcaatat ttgcagaaga
    10321 ttttgcctct ttttcctgtt cggtcattct tggcttttca ctttcccact ccaaatcatc
    10381 ttgtggcaat tccagtaaaa accggctcgg ctcaggtttg atgatttcgc catattggcg
    10441 acgttcttta cacagtgaaa acgtgagcgt ttgttgggct cgggtaatgc cgacataagc
    10501 caaacgccgc tcttcttcta cattgtcctc atcaaggctg gtttggtgtg gcagaatccc
    10561 ctcttccata ccgattaaga aaacgtgcgg aaattccaac cctttcgagg cgtgaagagt
    10621 cattagttga acttgatcgg cttcatcgtc ctcttcgcca cgttcgagca tatctcgcaa
    10681 ggttaaacgg tttaccactt gcaccaaatt catcggaggc tctatttcat cgccctctaa
    10741 catcgcttgc acccattcaa acaaggtctg cacattacgg ctttgcattt ccgctacttt
    10801 tgggttcgcc gccatttcat acaaataggc ttcatactga attttcgcca acagatcttt
    10861 tactgcttca atcggatcgg atctatcggc ttgatcggca atttccacaa tccagcgggc
    10921 aaaatcctgt aaggcttggt aaggctttgg cgtgaggcgt tgtatgagtt caaaatcaaa
    10981 aatcgcctca aacaaactga cctgcttttc attagcaagt atgcctaatt tttctagcgt
    11041 tgccgcccca atttcccgtt tcggcgtatt cacaatacgc aagaaagctg catcatcgtc
    11101 ttgattcact accaaccgca gatacgccat catatctttg atttccgctc gagcaaaaaa
    11161 tgacgtacca cccgaaattt tatacggaat gcggttttgc attagcaatt tttccaacaa
    11221 gcgggattga tggttgcctc gatacaaaat tgcataatct ttgtatttgg tctttttcga
    11281 gaaacgatgg gcaatcagct cgcccacaat gcgttccgct tcgtgttcct cattttttgc
    11341 ctcaatgacc tgtaattttt ccccttgccc cagttctgaa aacagccgct tgctaaacaa
    11401 gtgatcgtta ttatcaatca gaatattggc acaatgcaga atccgctggc tggaacggta
    11461 attttgctca agtttaatca cttgcaaatc aaaatcttcc cgtaaccgtt gcatattttc
    11521 aggcttcgcc ccacgccaag agtaaattga ttggtcatca tcgcccacga ccgtaaagtt
    11581 tggctcgttg ccgacaatca gcttaattaa ctcatattgg ctggtgttgg tgtcttgata
    11641 ttcatcaact aacaaatagc gaattttctg ttgccaacga gattttgctt cgggaaattg
    11701 gcggaacagc aacaccggca acataatcaa atcatcaaaa tccaacgcat tataagcctt
    11761 aagttgattc tggtagagct gataaaaatg tgaaaacact cgctcacgtt catcacgcac
    11821 acgagtaagt accctttcag gcgaaagcaa atcgtttttc cagttagaaa tagtggcaat
    11881 taaggctttc agcagatcct tatcttccgt cacattttca ggcaataaat gtttcagcaa
    11941 agcaatttga tcctgctcat caaacaaggt catacccgat ttaaaaccga gcaatttata
    12001 ttcgcgtttt aaaatttcaa acccaagtgt gtgaaaagtc gaaatggtta aacctttgct
    12061 gttttctttg ccgatagaat gatccacccg ttcacgcatt tcccgagcag ctttgttggt
    12121 aaaggttact gctgcaatct gcttaggcga atagccacaa tgtgcaatta aatgtgcgat
    12181 tttattaata atcacccgag tcttacccga gccggcacca gccagcacaa ggcaagcccc
    12241 tttggtatat tctactgctt gttgttgttg agaattgagt ttcattgaat ctattcaaaa
    12301 gtaattttga caactatttt aacggaaaga aaacaagcgg tcgaattttc aggaaaattt
    12361 gcaaaaaact ttgaaaatcc gaccgcttgt tcatcagaga aaaatcccca aaacgcaaat
    12421 ttttcctttc aatcttgccc ctttagctat atcattataa gttgattttt taggggcaaa
    12481 tgcctgattt tataactcct tttcttttta atttaaggga aaaaaacagt agatggaaaa
    12541 agtagaacag aaagctatca ttgagcttaa aaatgtaaca aaaagctacg gtagtaaaac
    12601 gattcttaaa aatttaaatt taaccattaa cgatggtgaa tttttaacaa ttttaggccc
    12661 ttccggttgt ggtaaaacaa ctgcgttacg tttaattgcc ggttttgaag atttaactga
    12721 aggttcaatt attcttgatg ggcaagatgt ttctaacgtt ccggcggagc aacgcccggt
    12781 aaacaccgta ttccaaagct atgccctttt cccacatatg acgatttttg aaaacgtggc
    12841 tttcgggctg cgtatgcaaa aagtgccaaa tgctgaaatt gagccacgtg tgatggaagc
    12901 attacgaatg gtgcgtttag aagaccgcgc tcaacaaaaa ccggctcagc tttccggtgg
    12961 tcagcaacaa cgtattgcaa ttgcccgtgc ggtggtaaat aaaccgaaag tattgttgtt
    13021 agacgaatcg ctttctgcgt tagattataa attgcgtaaa gagatgcaaa atgaattaaa
    13081 agcattgcaa cgtcaattag gcattacctt tatttttgtg acccacgatc aggaagaagc
    13141 cttaaccatg tctgatcgta ttatcgtaat gaacgctggt aaaatcgctc aagacggtac
    13201 accacgtgag atttacgaag agccaagtaa cttatttgta gctcgtttca tcggcgaaat
    13261 taacgttttt gatgcaactg taattgagcg tgttgatgca actaatgtaa aagcaaacgt
    13321 agaaggtcgc atttgcaata tcaaagtgga agatttagag gttcagccga accaaaaatt
    13381 aaaagtattg ttacgccctg aagatattgt gattgaggag ttagacgaaa acgaaagctc
    13441 gaaagcaatt atcggtcata ttatcgaccg taactacaaa ggtatgacat tagagtctag
    13501 cgtgagatta gatcataacg gtatgaatgt attggttagc gagttcttca atgaagacga
    13561 tccgaacatt gaccacgaag tgggacaaaa agttgctctt acttggtatg aaggctggga
    13621 ggttgtgtta aacgatgaag gcgaataaca aattccaaaa tggtgcggtc gcaactattt
    13681 tcggttggtt attacttttc gtattagtgc cgaacgtact cgttttaatc atcagcttct
    13741 taacagaaaa ccgtcaaagt cagtattttg tggactttgc atttagtttt gatgcttata
    13801 aatcactgtt taatgacacc tatgcagcag tattgtggaa ctcgctatat atggcgggca
    13861 ttgccacctt tttctgtttg atcatcggct atccctttgc attttttatt gccaaaatgc
    13921 cggaaagaat tcgtccgttt ttactgtttt tagtagttct accgttttgg actaactcac
    13981 tgattcgtat ttatggcatt aagattttct taggcgtaaa aggcttttta aatgaaagct
    14041 tgatggcttt aggtataatc agcgagccaa tgcgtttatt aaactcagaa attgcgatta
    14101 ttattggttt agtttacatt ctattacctt ttatgattct accgctttat tcatcaatcg
    14161 agaaaattga taaccgctta ctagaagctg caaaagactt aggtgcaaac gccttccagc
    14221 gttttattaa agtgactatt ccattaacaa tgccgggcat tgtcgcaggt tgtttattag
    14281 tattacttcc ggcaatgggt atgttctatg tggcagattt attaggcggc ggtaaaacac
    14341 cgttagtagg taacattatt aagagcttgt tcttaaatac caaccaattt gcattaggtt
    14401 cagcagtaag tattgcatta actatcttaa tggcattaat gctttatgtt tactatcgtg
    14461 caaacaaatt attaaataaa aaggtggagc ttgaataatg aaaaagatgt ttagaaacct
    14521 ttttatgttg gttgtttttg catatctcta tattccaatt atcattttag tgatcaactc
    14581 ctttaatgaa gaccgctttg gattaaattg gaaaggcttt acttggaatt ggtatgagcg
    14641 tttattcagt aatgatacgc ttgtacaagc aacattgaac tcattaacta ttgcgttctt
    14701 tgcggcaaca ttctcaacca ttttaggggc attaacctct attgcgcttt accgttaccg
    14761 tttccgtggt aaaaagctag tgggcggaat gttattcgtg gtaatgatgt cgcctgatat
    14821 cgtaatggcg gtatctatgc tcgtattatt tatgatctta ggtatcaagc taggctttat
    14881 ttcgctatta attgcacaca ttactttctg tttgccttat gtggtaattg caatttcctc
    14941 acgcctcagc gattttgacg gcaaaatgct agaagctgca aaagacctag gtgcaagtga
    15001 agtgactatt ttgcgtaaaa ttattttacc actggcatta ccttcgatta tttcaggctg
    15061 gttgttaagc tttacgattt cgttagatga cgttattgtt tcatctttcg tgacaagccc
    15121 aagctacgaa gttttaccac tgaaaatttt ctcattggtt aaaacaggtg taacacctga
    15181 agtgaatgca ctagcaacta ttatgattat tttctcatta acattagtgg tattaagcca
    15241 gttagtagtg aagaaaaaag tctaaaaatc actaaaaatc gtatacataa atcagcactt
    15301 taaagagtat taaagtgccg atttttttac aaaaataagc taaaaaaaca accgcttaag
    15361 aataatgaat tcttataaaa atagccataa ctaatcaata tctttacaaa gccgttaatt
    15421 tacgctatta attgcctacc aatataacta aaaagagtgg tgcttatgct gaagtttatt
    15481 tttaaacgtc ttcttgaggc gattccaacc ctactaattc tgattacctt ttcctttttc
    15541 ctgatgcggc ttgccccggg tagcccattt accagtgaga aagcctatcc gccgaaagta
    15601 atggcaaata tcgaggcaaa atatcacctg aatgaaccgc tgtataagca gtattttctt
    15661 tatatggaaa atctgtcaaa aggcgatttc ggtccatctt ttaaatataa agatcaatcg
    15721 gtaaataatc tgattgcttc tgcgtttcct gtttcgataa aactgggcat tactgccttt
    15781 atttttgcgg tgcttatcgg tttaaccgca ggaactatcg ctgcacttaa gcaaaatagt
    15841 aaatgggatt atttagtgat gggcttttca atgacgggta ttattatgcc gagctttgtc
    15901 tttgctccgc tgttggtact catgtttgcg atttatctca aatggttgcc tgccggtggt
    15961 tggaacggtg gtcagctcta ctatatggtt ttacctgttc tctctttaac cattagctat
    16021 gttgccggca ttgcccgtat tactcgtggt tcaatgattg aggtgttgca ctctaatttt
    16081 attcgcaccg ctaaggcaaa aggcttgcca atgcgtaaaa ttattttcaa acacgctttg
    16141 cgtccggcat tgttgcctgt gatcacttat ttaggcccgg cttttgttgg cattattacc
    16201 ggctcgatgg taattgaaag cgtgtttggc ttaccgggaa tcgggcagtt gttcgtaaac
    16261 ggagcattaa atcgtgacta ttcattagta ttaagcctaa ccattttggt aggtacgctc
    16321 accattttat ttaatgcgat tgttgatatt ttatatgcgg tcatcgaccc gaaaattcgt
    16381 tattcatagg agtaaagcgg aaaatgcaaa attcttcaaa aaatcaaccg cttgttgagg
    16441 ttacctcaac cattcaagga cgaagtttat ggcaagatgc acgccgccgt tttttccgaa
    16501 ataaggcggc tgtggcaagt ttgattattc tttgttgtat tattcttttt attacctttg
    16561 ccccaatgct aatgccgttt agctatgaag ataccgattg gaatatgatg gggattgctc
    16621 cggattttga atctcaacac tatttcggta ctgattcttc agggcgtgat ttgctggttc
    16681 gagttgcaat cggtggacgc atttcgttaa tggtagggat tgccggtgca ttaattgcgg
    16741 tgcttatcgg tacaatctat ggagcaattt ccggttattt cggcggtaaa gtcgatatgg
    16801 tgatgatgcg ttttctggaa attttaagct cattcccatt tatgttcttc gtaattttgc
    16861 tggttacgtt cttcgggcag aatattttcc tgattttcat tgcaatcggt atggtagcgt
    16921 ggctgaacct tgcccgtatt gtacgtggtc aaactctttc gcttaaaaat aaagagtttg
    16981 tggaagcagc gattgtgtgt ggcgtttccc gccgtcaaat tatttggcga cacattgtgc
    17041 ctaacgtttt aggcttagtg gtggtttatg cctcccttga agtaccatct ctgattttgt
    17101 ttgaatcgtt cttaagtttc ttagggcttg gtacacaaga gccgatgagc agttgggggg
    17161 cattattaag cgacggagcg gcacaaatgg aaacctcgcc ttggctactg gctttccctg
    17221 cctttttctt atgtttaaca ctgttttgtt ttaattttat tggtgatggc ttgcgtgatg
    17281 ccctcgatcc aaaagataaa tagaggttta gaatggaatt attagcagta aataatctcg
    17341 atgtttattt aaaaacggac gaagaattag ttcacgcagt gcgtaatgtc tcgttctcga
    17401 ttgaaaaagg gcaaaccctt gcgattgtgg gggaatcggg ttcgggtaaa tccgttactt
    17461 caatggcaat tatgcagtta ttgcctaaaa atatcgtctc attcggtgaa cgctcaagca
    17521 tcatttttga agaacaaaac ttgcttactc tatctgaaaa agcaatgcaa acagttcgag
    17581 gtgatcgcat cggtatgatt ttccaagagc caatgacctc actcaatcct tatatgccga
    17641 ttggcaagca ggttgctgag acggttcaaa cccataatcc gcaaatcagc aatgccgaag
    17701 cggaaaaaat ggtgttggaa actttgcaaa aagtaaaaat tcccgatgcg gaaaagaaaa
    17761 tgatttgcta tccgcacgag ttttccggcg gtcagctaca acgaattatg attgcaatgg
    17821 caattatcaa caaacccgat ttattgattg ccgatgagcc gaccaccgcc ctggatgtca
    17881 caacccaagc ggaaattttg gatttaatgc acgacttaca gcgtgatatg ggaatggcga
    17941 ttatcttaat ttcccacgat ttacgcttgg tgcataaata cagtgactta gtctgcgtaa
    18001 tgcaaaacgg cgaaattatt gagcgaggcg aaacggaaac tgtgttcacc aatccgcaac
    18061 atccttatac ggtggagcta cttaccccga ttccaactaa tttaaaaggg gaattaccgc
    18121 caaatgcacc aaccttgctt cagggtgagc atattaaggt agattatatt ttaaaacgct
    18181 cgctattcgg taagccgaaa aaggtgttta atgcgttaaa agatatttca ttgcacttga
    18241 aaaccggcga aacgctcggc attgtaggcg aatccggctc cgggaaatct acccttgggc
    18301 gtgccattat gcagatttta gattatcgcg gcaatattac atttgcaggt aagacactgg
    18361 ctaatctgaa taaagccgaa cgtcaagccc tgaaaaaaga tatgcaaatg gtgttccaag
    18421 acccgttcaa ctcactttct cctcgcttaa cagttgggga aattattgct gagggcttaa
    18481 ccgtacattg ccctcaaatg agcaaaaccg aacggcggga aaaagtaatg aaaatgttgg
    18541 aagaggttaa tctcaaccct gcaatgatta accgctatcc gcacgaattt tcgggcggac
    18601 aacgccagcg gattgcgatt gcaagggcaa tcattttaga gccgaaattt gtattgcttg
    18661 atgagccaac ctcagcactt gaccgctcta ctcagatcac tgtaattgaa ttgcttaacc
    18721 gcttacagaa aaaatacggc ttaagttata ttttcatcag ccacgatttg gcggtcatca
    18781 aggcacttag cgaccgagtg attgtaatga gccaaggtga gattgtagaa agcggtgatg
    18841 tcactcaaat ttttgaaaat ccacaacaag aatacaccaa gcggttgatt ttagcctcta
    18901 atttgtaacc ccttccacaa ggtcttattc taccaaaaaa caagaccttg tcattttatg
    18961 ctagcaaaac attgtaattt tgatatttta tttcaatttt ttgaaaaaat atggaacttt
    19021 atataaaact cactttctaa acaatacgtt atctgaacta agataacgct tatttaaagg
    19081 aaaatatatg aaaaaattga tgatattcgc aacaactgcc atgattgtaa gcaatttagc
    19141 ccatgcagca ggtgaacaaa gtgatgcaag agaagcccac gaaagcaccg taaaaacctc
    19201 aactgttaag tatagctgcc aaaacggcaa aaaattaagc gtgaaatacg gcttcaataa
    19261 acaaggcatt ccaacctatg ctgaggcaaa attaagcggt aaaaaacgtt ttatgccgat
    19321 caacctatac acgacagatg caaccggcac taactttggt gacgaaaaca atttcagcct
    19381 ttacggtgat ccaatggaat ttaccaacca ccgtaaagca tcagtaaata tccaaagccc
    19441 cgcaagtgaa attttatata aaggttgcac acctcaaaaa tagataaaaa gtaaaatgag
    19501 tccgctctga taagtggctt tttaatagct gtttattgca gataggaaag cattttgttt
    19561 ttatctacta tccagaaaca agcggttaaa tttgtttaat tttttacaaa aaatgggcca
    19621 aaattaaccg cttgtttatt actataaaga aataagtacg taaaaattaa ctcttacatt
    19681 taatgcttat ctctaaaatt taaactccac tgatgccatt acatagcgct ctggagcaag
    19741 gtaacgattt aagccttcgc cagttatact gttggcaact ccaggactag ttataccaat
    19801 agttcttaaa ctatcccaag tccaatattt tttattaaag agattataaa ccccaacttt
    19861 aacaatcgct ctttctccaa actttttgtg tgcaactaag tcaaatacgg tggctgaatt
    19921 acttaagtat gggtaagtgg ttaatgattt atcgtaactt aaatacccag tattggctaa
    19981 aatagcatac tgttgtgcat attttgcttt tttgccttgt gtgtatgtgc cgtttaggtg
    20041 tatcgcccag tcattgttca cagctcgata acctatgccg aacaatgctt ttaacggttg
    20101 aaccgtgagt aagtcggtac catcattacg tttgccacga gcataaccaa atccaccatt
    20161 aagctcaaag ccttgtggaa ttgcattcca cgctgtgttt aagtctaaag ttccgttaat
    20221 atcaacacct ttgattttag ctcggttaac attttgcgat tggtaaatat tgttaccaat
    20281 agtggcaaga tcaatcagat ttctatagtg ggtatggtaa gcggctacgc tcaagctacc
    20341 taatgaacca ttacctacca gtgaaatttc attacctaag cttgtttccg gttttaagtt
    20401 agtatttgct aagaaagttt ggcttgctcc acgcccttga acttcacggt taaagaacat
    20461 ttcacttgcg gtaggtgttc taaaaccacg gctgagttta tagccaattt gccatacagg
    20521 agagagctga taatttaaac caatatctcc cgcccaattg ttaaaggttt tatctgacaa
    20581 actattatca tcagaatgta atttcgactg ctcatatcga gcatttaaat gagcagatag
    20641 attttgtgtc agtaaaatag agtctcccat caaaatatgg aaagacttgg tttttgccgg
    20701 agactgcata ttttgatcgg ttggcgtaat acctgctgta taggtttgca tatcaaaact
    20761 gaaatcagat acaccatagc ctaattccgt tgacaattta tgttcggtat tacctagatc
    20821 aacaggcttg aatgtaacac ctaagctacc ttcaacattt ttagtattaa aagcccgttt
    20881 ggtattatct gtttttgtac cacgggcaat agtgatttga tcgttaatac ctgaaatttc
    20941 agtatcctgt ttggttaccg caactttaat attttcaaga atactgtttt ttggagtgta
    21001 taaataagta acgccataag tttcaatttc ctgcacatcc aaaaaatcac gtaatgtgac
    21061 ggtagaataa gaaagctctt gaccggcgtt tttactgcgt tgcttagaag tttttaattc
    21121 aaggcgattt tcatcattcc attttagccc taattttgcc aagtagctgt attgattatt
    21181 tttgtaagga tttggtaaac cacgaccact tcccataata tccggacctt tagacgttgt
    21241 ttgtgtttcg tgtccttgac ggtggctata cataaccaaa gcatccgcat attgaccatc
    21301 cgcagcaata cctgccgtct caatattctc tttattttga ccgttatagg tatatttcaa
    21361 catcccacca aattggcgac ctttcattaa aatatcactt acggttttag ttttatagtt
    21421 cacagagccg cctaatgccc cattgcctac ttttgtgctt gagccttttt caatggttac
    21481 atcgctcatt aattcagtat cgatttcagg gcgagattta ttgtagtaac tatagcgact
    21541 aaaggtggca tagttatttg atgctgcttg cgatatgcca tcaactgtta ctgccacttg
    21601 attaccttca acaccacgga tattaaaccc ttgtgggcca aagcggcctg tttgcggaac
    21661 gcccatatca gtactataac gtactaaatc ttcattgctc atcaccgctt gctgtttaag
    21721 taggttctcc tgctgctcat ttacttcaat agtatctagt tcaatgctac taatctttgt
    21781 ttcttgagct aaggagagtt gagaaataaa gggagtaatc gctattgcta atacagtttg
    21841 ctttatttgc atattttaga tcctctagtt agtttaatcc gtttaatgtt aacactatat
    21901 cattttaatt gaaaattgaa ataataatta ttattattta agtttagata aaaatcaaat
    21961 ggagtataaa atgagaacct tacttttttc tttcattgcc ctatcaatgt gtttaaatac
    22021 cacaaaggcg gcagaaaaaa ttgtttcaac tgcgggctat gcttccgaaa ttgtcgcagc
    22081 actaggaaaa gctgataagt tagtcggtgt ggataccact tcagttaaac ctcaaacgat
    22141 aatggagaaa aaacctaaaa tcggttatcg tcgccagctt tctgctgaag gtattctctc
    22201 tttaacccca gatttgatta ttttagcccc tgatgccggc cctaacgcag ttattgagca
    22261 aattaaagcc agcggtattc cattacttca cttaggagag aaaccgagct tagcagggat
    22321 tcgtgaggat attcagcaaa ttgccaaggc gattggtgca gaatcagagg caaaacaatt
    22381 aacagaaacc attctggcag atgaaaaaaa attagccgac attcgtagcc aacattctac
    22441 tgacaaagaa gtcttagtgc tgattgacac tgcatcgcaa ggtatctttg ggctagggaa
    22501 aaagagtgtg ggtgatcatt tcttaacctt gttaaatcta aacaatagtt tcaatgctga
    22561 gggcaataag ccactcgcca atgaggcttt agctgcaagc aatgccgatg ttattttatt
    22621 agcctcccgt aatcaggcaa aagaatctgc aacgatcagt aaattagcag aatccagccc
    22681 acaatacgcc caactcacaa atacacaagc gggcaaaaaa ggctgtgttt ttgcaatcaa
    22741 tattttagat gccttgggct ttgggcctta cactgctcaa tatgcacacc aaattctcac
    22801 cactattcaa ccttgtttaa aataacggat acgctttgaa actgcattta accttaattt
    22861 tcactttaat tatcagctta gtgatttgta cccatttagg ggcgatgtca ttacccctat
    22921 ccgcttggca ctgcaacaac gctgaatgcg aaagtgcagc ctatatttta tggcaaatcc
    22981 gcctgcctcg attactaggg gcattattca tcggggcagc cctcgctatt agcggagcaa
    23041 caatgcaagg gctgtttcgc aatccgttag tagatccggg gattattggt gtctcttcag
    23101 gtgctgcatt ggctgcggca ttatatattg tggtgattca gccactactg ccggaatcaa
    23161 tcagctatta tgctctacct attgccgcct ttttaggggg ctggctggtg acttttatgc
    23221 tctatttact ttctcaacgt aacgggcaac tacatatcgc cattatgcta ttaattggta
    23281 ttgctctagc tgcatttagt ggcgctttaa ccggcatact gatttatgtt gccgatgata
    23341 accaacttcg tagtttaacc ttttggagta tgggatcttt agcaggtatg aattggcaaa
    23401 tggtggcaat gatggctgcg gttgtcttca tttgtagccc acttatttgg cgggaacacc
    23461 gtgcgttaaa cgccttaaca ttaggtgaag aagatgcaca atacattggt tttgacattg
    23521 caaaagtcaa acgtcgtctt gtgttttacg ttgccttatt aaccggcaca agcgttgcct
    23581 gtgttggggc tatcggcttt gtcgggctgg ttattccaca tttaatgcgt ttactgggag
    23641 gcagtaacca ccgccattta ttactcaata gtttggtttg cggtgcaatc ttattaatga
    23701 ttgcagatac tcttgccaga accctagccg ccccggcaga aatcccgatt ggtattttta
    23761 ccgccttatt tggtgcgcca ttccttgcag taatggtatg gaaaagtggg aaacatatct
    23821 aatgagcaat ttattaactt gccaaaattt aagcatcgtt catcaacaaa aaaagttgct
    23881 ggagattcaa gaaattgcct ttgaacaagg aaaatttacc gccatcatcg gtccaaacgg
    23941 agccggcaaa acaaccttgc tcaaagcctt aagcggtaat aatgcacaaa ctaacagtgc
    24001 cgtattttat caacatcaac gcttaacaga cttaaacgga tatgctctgg ctcaaaaacg
    24061 ggcaatgctc agccaacaga gccaaattgc ctttccctta aaagtagaag aattggtaca
    24121 actcggaaga gagccatatc gccaaagtga atttgcaaaa tttaatgaaa aaataaccgc
    24181 ttgggctatg gaaacaatgt ctttaaccgc attaaaagaa agaagtagca tacaactttc
    24241 cggtggagaa caacatcgag cgcatattgc tcgagtgctg gctcaactcg ttcctgaacc
    24301 gaatgccgat ttaagcggaa gctggctttt gcttgatgaa cccaccaacc atttggatat
    24361 tcatcaccaa tatcagcttt tttccttact acaaaagctg aaacaacaag gcttaactat
    24421 cattgcgatt ttgcacgatc ctgcattggc aataaatcaa gccgatcata tcttaatgct
    24481 aaaaaatgga gaaaaatttg ctgaaatgtc cgctaaagcc attgcagaaa gttgtttatt
    24541 agatgagctg tatgaaatga aaatgggctg tcgctattgc cctcacagcg ggcaatatta
    24601 tctttgccct gcattagctg aaagttaggg tgcgatgcac cctatcgttt aatcttctgc
    24661 atatccacaa gcaggcagta gtttaccgtc taaatacgct ttgccgtgct gagaaactaa
    24721 tctacccgaa cagaaccact caatcaccaa cggataatat cgatgttctt gctcaaccac
    24781 tcgagccatt acatcttcaa tctcatcttc aggataaatc ggcactttag cttgtaatac
    24841 caccgcacca gcatccactt cttcattcac gaaatgaata gtcgtgccgt gttcactttc
    24901 cccagcctct atcgctcttt gataagtgtt tagccctggg tatttaggta ataaggaagg
    24961 gtgaatattg agtattttgc cagcaaaatg ttgcgtaaat tcaggcgtta aaattttcat
    25021 atagccggct aatacaatta actcagctcc taactgctca atttgttcgg caatcgcaag
    25081 atccatttcc acattgcttt ggtaatcttt acgagaaaat acaaatgtcg ggattcccgc
    25141 ctgtttagct cgaatcagcc cataggcatc tgccttattg caaattacac cgcaaatttg
    25201 accgcttgta tccgcagatt tttgcgcatc aatcattgct tgtaggttag aaccattccc
    25261 tgaaattaaa accacaaatt ttttcatatt acacaccggc tgaatattca tcgttctgga
    25321 tattatgcct tgaatctcaa catcagtaca ggaaagaaaa agggcgtatt ggttaaaata
    25381 agccactttc aagctagaaa cgcacctctg cccctagtgt gtaagttcga cctctggcac
    25441 tatttgtgaa acgataggtg tctgtatcat agtcatactc ttctgcggtg gaagagttat
    25501 acgcatttaa ggcatcaaga taatttcggt tcatcacatt ttgtaccccg gcttttaagg
    25561 ttaaatgttt attcactttg taatttgcat ataaatcgat aatcaccgga atattcggta
    25621 actgctcgtg aataatgttg ccatcttcgt ctaaatcaat accgacacgt ttcgctttgc
    25681 ctgtatattt aaaaatgcta gacagtgtta attttttatc cagcaattta gcccctagct
    25741 ccaaagtcgc ataatctttt ggtagctggg tataagcttc agtgccaaat ccataaagaa
    25801 tagtcgcacc ttttgaagtg ggtgctgtgc tttcagttcg ggtataacta ccattgacaa
    25861 aaaaagcatc ggaatcgtaa gccagctcaa cttcccaacc tcgattttta accttttcag
    25921 ggtgattcac ataaatttgg aactgtatag cattggtagc atcacttgaa tttcgacata
    25981 ctgttggccc acaaccatac aacgagaggt tatacaaata gtctttaatg cgtgagttaa
    26041 aatataccgc ttttagcccc aagatatcat tattgagtaa tacattatct tgatgaatat
    26101 taaacccaat ctcataggtt ttcgcacttt ctggtttcaa aaacgggttc acactcaagc
    26161 catttcgact ggtattaaac atttcttgca cattaggcga acgggaggtt tgtttgtaac
    26221 tgacaaaagg ttgaaaccaa tcagcaacct gaaatgctaa agtggcagaa ggattcaact
    26281 tataatgttt tttattaaca tcaaacccaa aagaagggaa acaataatcc atatcatcgc
    26341 tatcgcattc tggcttatat ccttttaact gatagcgtga atagtgaaga cttgaattta
    26401 aagtgacaat gccgtatttc cattcggtgt ctaggtaaag ggaatcaata ttttgcttac
    26461 ctgccggccc aaaagcggca ttgctaatcg ctccatcatt attcttactt tccggtaaat
    26521 cacgggaata tttatttccc tgactcaaaa cggctggtgt tattcaaatc aaacatccac
    26581 attttgtttt tggttgaggc atcttctaat gtccatagtt ttgcatcatc gtggaaatct
    26641 tgcttagttt ctgttactgc tgttaggaat ttcagatcaa tccaatcaga ttgaggctgg
    26701 taactggatt ctaaggaata ggtcacacta tcaatattac gccccactaa gctattttta
    26761 tattgacgtc cggacaattt aaactctgaa tcttgatttg gcttaaaggc aatttttccc
    26821 atataagatt cagggtgatt ttttaaccct tgcatataag cagaatccgt tgccattgtg
    26881 ccattacctc ggcgataatt ttgactgata ctacttttgc taaaagccaa taaactgcca
    26941 agagaaccac tttccagcac atcaactttt ccggctagtg caagcattcc gttttttccc
    27001 agatcattat tgccataatt aaaacggctt aatacgccga ctttatgttg ttctagtaga
    27061 atatcttcga catcaatcgt tttaaaatta gcactaccgg ctaaagcatt caatccggct
    27121 gcaccattgt tgtagccttt cgcaatatca actcgggtta aaaaattttg atcaattaaa
    27181 gtgctgaatt ggctcattgg cccgttacca tcagtgtgga acttaccacc tccgtttgct
    27241 gatgtaccaa gctgtgtttg tggcacgcca tcaactaagg tatttacccg accaagacca
    27301 ctcatattac gaatattcac actcacgccc ccttgccccg ggtcaatttg ggtatagcca
    27361 cctgcgacag aacggacaat actatctaaa ctttgcattg cattcacatt cattgctcga
    27421 gaactcaccg agccggcttt actgaaattc tcaaaattag ggttttccac agtgccataa
    27481 accgtcacag tatcaagctc cgcaatattt ttcgcctttt cctctgccaa tgcagtttgc
    27541 gatcctccca tacacaaaac cggaaacaaa atgcgtgtga ttatattaaa tttcataatt
    27601 ctccttattg agtgacccaa agataatctg cctgtgaaga agtaatttct tctctctctg
    27661 ttttaagcat tagcaccgct actttttttg ttcggtcata ttccgcaata tgcaacggaa
    27721 cgccaagatc tttgcgaacg tggtggttac ctgcaatcag caagctagtc gataacgcat
    27781 tttttactaa tttttctgcc attctgcgat cacggaattg ttgtactgcc accatatttt
    27841 ccatcgggta aggcattccg tgattcattg acagcaacag ttgtgcgatc ttttgcttaa
    27901 cttcagagga agtggataca gaacctttta atggctccgc cccatttaat aaaatttcaa
    27961 cctctttatc tgttaagtta gtggctataa cacgactatt tgaacctaag ctctctacca
    28021 ctaaatcttg atacatcttc caatcccatt tttgccattg aattgcctct cttaaatccg
    28081 tggctgataa attaaccggt cggttaaact gaatgcgatc aatcgcaggc tgctgatcca
    28141 catttaacat ctccaacgca attaaatgta acttggcttt cttcgtaagt tgattaaata
    28201 aatacaattc aatttggtgg tgaattaact gagtatgctc ctcaccaact aaaacgtaat
    28261 cttgagaaga tagacgggaa agtagttcag taaatgaaat cgtttgattg gtattcagat
    28321 caataatttg gccgatagag tccacgtttt gttcattaag ggattgcgtt gaaaccgagc
    28381 agcctaaaag aaaaaaagcc ataaaaaggc tagctaaaaa agaggaaata cgaggtaaca
//
LOCUS       DANLZC010000013        28388 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.013, whole genome shotgun sequence.
ACCESSION   DANLZC010000013 DANLZC010000000
VERSION     DANLZC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28388
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.013"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            61..1929
                     /locus_tag="PXE44_000459"
     CDS             61..1929
                     /locus_tag="PXE44_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL5588002.1"
                     /translation="MSGLVSPELIKTVALLATSVTIVPLFKRLGLGSVLGYLVAGCLI
                     GPSGIGLFQDPAAVVHMAELGVVMFLFIIGLEMHPELLWAMRKAIFGRGFLQVAICGT
                     LLTLAGIYLLGFSKEVAFIAGMGFTLSSTAIVMQVLEDKGISSTPKGQRIVATLLFED
                     LAIVPLLASVAFLAPNQVDAESSTNWSAIGLSLVAVLILVASGKWLMNPIFRVISKAK
                     VREMMTAAALLVVLGSALLMEISGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGLL
                     LGLFFMGVGMSLDLSLVLNNWLWLLVIVAVYVIGKGLGIFIAASITRLTLNESIVRTT
                     IMAHGGEFAFVLFSAAATAGILTPDTHATLTAAVIVSMLLSPLLILIKQGVRKKKAKN
                     ESTNMTGVELPEDLEANVLVIGFGRFNQIVCQALLAKGLSVSVIDSNTDHIRAAARFG
                     FKLYYGDGARLDVLRASGIANANCVIVGVGNPERTEKIVELIKSEYPLVPVFARTYDR
                     QSAVDLVKKHHVDYHIRETFESALALSQAAIERLGSSEEEAKQIISYVRRLDAERFNE
                     ELLHGFSAEVIRKYWVPTPWVNPQHEGSALNEETADILEELGETVEVIKVDITDKN"
     gene            complement(2089..2985)
                     /gene="malM"
                     /locus_tag="PXE44_000460"
     CDS             complement(2089..2985)
                     /gene="malM"
                     /locus_tag="PXE44_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDL5588003.1"
                     /translation="MKKTLLSTAIFFAHLLVVSPSIANGSAGKSIHINSAELAQIQWQ
                     DVPFSQTVKTDLSEQHKQAFTTAFAGIASPVAAYRIPANQGTLEIEIESPVMDKNVFV
                     PTAVVLDSQFNVASTYPSSEFKLLEERGLKGSRLGAELNLTPAMNQDYIYLLIYTTQQ
                     DLAKTTMMPHPAKTYAKATGKQPPAINDIEVAHSLKGQIHINVTGSNGTKFIGLPTEI
                     FSSNKAATPAAKTAIPLPIKENPVAVKTAVDKDTEAYFNQAVMKAIKAKDINKAMNLV
                     NEAEKLGLTSPRQIFLKNVSSN"
     gene            complement(3066..4355)
                     /locus_tag="PXE44_000461"
     CDS             complement(3066..4355)
                     /locus_tag="PXE44_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin"
                     /protein_id="HDL5588004.1"
                     /translation="MKKTVLAVAISGAMFAASASAVDFHGYARSGIGWTSGGGEQSAF
                     TVNGGGSKYRLGNESDTYAELKLGQELFKSGEKSIYFDTNLAYGGTLHNNDWTPTSPA
                     LRELNVQFKNFADSLPGATLWAGKRFYQRHDVHMNDFYYWDISGPGAGVENIDLGFGK
                     LSLAVTRDTERGGANTFGYTTVYKLDPSTGKIKLDKQRNKADVYNDIFDVRLAGIELW
                     KDGSLELGFDYGNAHAKKGAALEKGATKNGYMVTAEYTQGNFFGGFNKFTAQYATDSM
                     TSWNNGHAQGSKADNKGNMLRLINQGVVQASDKVEVMYALIYEKTKLDNKQGKTWYSA
                     GIRPMYKWNDTMSTLLEVGYDRIKDQATGKKNDLVKYTVAQQWQAGNSIWARPAIRVF
                     GTYARWNDKFNTKARTDAGYKAKDGEFITGVQFEAWW"
     gene            complement(4453..5571)
                     /gene="malK"
                     /locus_tag="PXE44_000462"
     CDS             complement(4453..5571)
                     /gene="malK"
                     /locus_tag="PXE44_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDL5588005.1"
                     /translation="MANVSLRNVGKSYGDVHISKDINLEINEGEFVVFVGPSGCGKST
                     LLRMIAGLEDITTGELYIGGKLMNEVEPSKRGIGMVFQSYALYPHLDVAENMSFGLKL
                     AGVSKAERDQRVNQVAEILQLAHLLDRKPKALSGGQRQRVAIGRTLVSQPEVFLLDEP
                     LSNLDAALRVQMRVEISKLHKKLNRTMIYVTHDQVEAMTLADKIVVLNAGGIAQVGKP
                     LELYHYPANRFVAGFIGSPKMNFLPVKVIAVEPERVKIELPDANHHNFWIPVSGEGVN
                     VGDNLSLGIRPEHLVPSDQTQVTLHGIVQVVELLGNETQIHLEIPEIKQPTLIYRQND
                     VVLVNEGDDLNIGILPERCHLFKEDGTACKRLFVEKGV"
     gene            5941..7131
                     /gene="malE"
                     /locus_tag="PXE44_000463"
     CDS             5941..7131
                     /gene="malE"
                     /locus_tag="PXE44_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDL5588006.1"
                     /translation="MKNKCVKFTLATLATLVLSGTVMAKMTEGKLVIWINGDKGYNGL
                     AEVGKKFEKDTGVQVLVEHPDRLEEKFAQVASTGDGPDIMFWAHDRFGGYAQSGLLAE
                     VNVSKEFKDKFVDFAWDAETYNGKIIGYPVAIEAISLIYNKDLVKEAPKSWEEIIELD
                     KKLKKEGKNAIMWNLSEPYFTWPIAASNGAYAFKFANGKYDPKDIGVNNEGAVKALQF
                     VVDMVKNKHISADMDYAVAEASFNKGQSALTINGPWSWGNIDKSGIKYGVTLLPTLNG
                     QASKPFVGVLSAGVNSSSPNKDLAKEFLENYLLTDDGLDAVNKDKPLGAVALKSYQEK
                     LAADPRIAATMANAKNGEIMPNIPQMSSFWYAEKSAINNAVTGRQSVKEALDDAHSRI
                     QKQQ"
     gene            7250..8788
                     /gene="malF"
                     /locus_tag="PXE44_000464"
     CDS             7250..8788
                     /gene="malF"
                     /locus_tag="PXE44_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDL5588007.1"
                     /translation="MLTQTQSKSTHWLKYLLAGLVLLLDFYLVVLMYSQGEYLFAILT
                     LIILTSGVYIFTNKNTYAWRYVYPGITGMAIFILFPLVATIAIAFTNYSGSNQLSFER
                     AVSVLTEQRYFAGDKYQFTLYPQADNKYQIALTNPTTEQTFVSEPISLATGTNVVVTE
                     QNRPTGEVAPLKVITQNRAALQSMKVVLPNNNELTMSSLRQFSEQKPRYQFDEENKIL
                     LNNETGKRYQANDETGFFQSIDENGHWQSETLEPGYTVTSGFNNFIKIFTDEGIQKPF
                     VQIFIWTVIFSLLTVVFTVILGMVLACLVQWEALKGKAVYRVLLILPYAVPSFISILI
                     FKGLFNQSFGEINMILNQLFGISPEWFNDPLLAKVMILIVNTWLGYPYMMILCMGLLK
                     AIPADLYEASAMDGASTWQNFSKITFPLLLKPLTPLMIASFAFNFNNFVLIQLLTNGR
                     PDMIGTTTPAGYTDLLVSYTYRIAFEGSGTQDFGLAAAIATIIFLLVGGLALLNIKAT
                     KMEL"
     gene            8841..9731
                     /gene="malG"
                     /locus_tag="PXE44_000465"
     CDS             8841..9731
                     /gene="malG"
                     /locus_tag="PXE44_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDL5588008.1"
                     /translation="MAIVQSKSVRYRVWATHFILICFLALIIFPLLMVIGISLRPGNL
                     AIGDIIPSQISWEHWQAALGFDVTHADGTVTPPPFPVLRWLWNSIKVATITSIGIVTL
                     STTCAYAFARMKFKGKKTILQGMLIFQMFPAVLSLVALYALFDRLGQYVPFLGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDGSLEEAASLDGATPWQAFRLILLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLYPQNYLWGDFAAAAVLSAIPITL
                     VFLLAQRWLIGGLTAGGVKG"
     gene            9806..11863
                     /locus_tag="PXE44_000466"
     CDS             9806..11863
                     /locus_tag="PXE44_000466"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDL5588009.1"
                     /translation="MKNALPYLCLFYTSSLFAQSWQHSHFSDFNDDLEKHFFQSQVQL
                     EKGSYPLRFKFGEQCYQPQQAIKLNQSVALVPCINEAPQLRLFRQGNYLAQIDLRSGT
                     PTPTISVEQQLQHNEAMFQSCPNWDKKPIEIDVSSTFAEGESVSDFYSGNTAIVKNGK
                     VTLMPNENASGLILLEKSNTENTATFDWKNATVYFVLTDRFYNGDPTNDHSYHRQKDG
                     MQEIGTFHGGDLKGLTEKLDYLQQLGVNVLWISSPLEQMHGWVGGGDKGDFPHYGYHG
                     YYHLDWTKLDANMGTEDDLSRFVQQAHQRGIRVLFDVVMNHTGYATLADMQEFNFGGF
                     YLTPDEISRTLGEKWTHWKPKSGQNWHSFNDFIRFNDNQAWQNWWGKEWVRADIADYD
                     SPKFDDLKMSLAALPDLKTESEQAVKLPEFFANKNTNAKTLPNAKVRDYLIGWLSDWV
                     RKYGIDGFRVDTAKHVEKSTWLVLKQSAQQALNEWQKANPNAGFNDRFWMTGEAWGHG
                     VFKSDYYQNGFDAMINFDFQDQAKQGLNCFAHIEPIYAEMNRKLSDFNVLSYLSSHDT
                     RLFFHSDSEQNIEKHKTAANLLLLSPGAVQIYYGDESGRTFGATGSDLIQGTRSDMNW
                     QELQQDPQKQALHQHWQKLTQFRQRHPAIGAGVHQLIKNDSYFAFKRVLNEDKVMVVW
                     AGN"
     gene            complement(11977..14703)
                     /gene="malT"
                     /locus_tag="PXE44_000467"
     CDS             complement(11977..14703)
                     /gene="malT"
                     /locus_tag="PXE44_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDL5588010.1"
                     /translation="MLIPSKLVCSFRLQNSIPRKRLIQELDKSCSYPVVLINAPAGYG
                     KTTLVSQWIENKKNIGWYALDEGDNNSDRFATYFSAALHSAINEEIDVLLEENRKANL
                     LALFNQLLIKAAAFSEHFYLAIDDYHIIENDEIHEALKYWIKHQPSNMTLILISRSIP
                     PLSIASLRVQEQLLEIDINQLMFDHQESTAFFQARLGSELNQQDVIALCNEVEGWPTA
                     LQLISLFAKNKSHTLQVPLQDIAKRLAKSNHFHINEYLADEVLNKVDEETRLFILRCS
                     VLHSMNESLVQAVTGEQNSRTKLESLEKQGLFLQQMANSKWQSSDDSWWKFHPLFASF
                     LNLCCQNELYAELPQLHQKAANAWLQLGYVTEALHHAMQLSDTGLLLNILSKHAWTVF
                     HQGELKLLEESLNCLDYAHLTEHTNLVLLKAWLVQSQHRHIEVGGILADFSRALTENK
                     IELSKTANAEFNVLRAQVAINSGDENTALQLASEALNDLSENAYYAHIVATSIIGEAH
                     HCHGNLAEALAMLQKAERMARQHHTYHNILWSQLQQSEILLAQGFSQAAYEMLDKASE
                     FVKENHLQKVPMYEFLLRSKGKILWEWYNLDKAESMAVAGMNALSKFEDKLQCLTLLT
                     KISLARGNLDNTARLLGEVEQLEHSHSYHHDWIANADQVRIFYWQMTNDVSAVRNWLI
                     QNPSPVSDKNHFTQVQWRNIARSRILLEEYHEAKAILDNLIETAEKFSLISDLNRALI
                     VRNRLYFLQGEKELAQQDLIQALKLTRQTNFISAFVIEGDVMAQQIRHLLQLNVLDEL
                     VLHKAQFILRNINQFYRQKFAHFDENFVNQLLKNPKVPELLKISPLTQREWQVLGLIY
                     SGYSNEQISDELQVAATTIKTHIRNLYQKIGVTNRNEAISYTKELLMLMGYH"
     gene            14867..17236
                     /gene="malP"
                     /locus_tag="PXE44_000468"
     CDS             14867..17236
                     /gene="malP"
                     /locus_tag="PXE44_000468"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDL5588011.1"
                     /translation="MTFNSIVEKYCRYFDVTDPKHFTLQQWYQVVAEGSLELIYSQPA
                     TKPTESRHVNYLSMEFLIGRLTGNNLMNLGYYEQIRDYLKQYQVELVDVLEQERDPAL
                     GNGGLGRLAACFLDSMATVGQNATGYGLHYQYGLFKQSFKEGMQKEAADTWARDSYPW
                     HRFNPSKTRHIGFGGKIKHIQADQYEWQPKLTIQGKAFDLPIVGYKNNIIQPLRLWQA
                     DSDQSFDFDAFNEGKFLSADKTIVNAAALTQVLYPNDNHQAGQKLRLMQQYFHCACSV
                     ADILDRHISEGYSLEDLAKRQVIQLNDTHPTLAIPELMRVLLDEHQFTWEQAWDICSN
                     TFAYTNHTLLPEALEQWDQRLFKQLLPRHYQIVEKINDIFHDKVQAEFGDDAKIWEKL
                     AVLFDYRVRMANLCVVTCFRVNGVAEIHSDLLVTDLFPEYHKLFPTKFCNVTNGITPR
                     RWIRQANPQLSDLLDRTLKQDWTHNLELLKGVEQYADDTGFREEYQRIKRNNKVVLAN
                     EIHKTLGLNVNPNAIFDVQIKRFHEYKRQHLNLLNIIATYQSLKANPNQDYTPRLFLF
                     SGKAAPGYYLAKNIIHAINNVADIINNDKQVNDRLQVAFLPDYRVSLAEKIIPAADVS
                     EQISMAGKEASGTGNMKLALNGALTLGTLDGANVEIADMVGEENVFIFGHTVESVREL
                     LAKGYQPKAYYNQDPILKNAVDFLATGKASNGDKEAFKLMLDSLLERDPFLVFADFDS
                     YRQAQEKIGVAYLDQETWLRSAILNTARLGAFSSDRSIKDYQQRIWLNR"
     gene            17318..19396
                     /gene="malQ"
                     /locus_tag="PXE44_000469"
     CDS             17318..19396
                     /gene="malQ"
                     /locus_tag="PXE44_000469"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL5588012.1"
                     /translation="MKITAKQYYRAGIMPYFFDERGVKKTASEKIKHSLFKAFNGNTQ
                     ARETPIPTVNIFYQHQPCFLPFHLADKKHPLTGTWQLQLENSNQIISGKIKARGITLP
                     QDLPLGYHQLQLKTVDKTFNCTIIIAPERCYQPQAVIEQKRLWGTFLQLYTLKSAHNW
                     GIGDFGDLKDFIQQIAPYQADFIGLNPIHSLFPANPNSASPYSPSSRRWLNIAYIDIN
                     QLAEFQQSDEAQTWFHSAEIQQQLSELRTQDWLNYEQIIPLKLKGLRFAFAKFQQNMT
                     ACAAPAFIEFVQKGGESLQVQATFDALHQHLSAQFADQWGWDFWEKAYQDYHSAAVQQ
                     FRETHKTDIEFYLWLQFVADQQLAECDAICRQQNMRIGMYRDLAVGVSPNGAETWNDK
                     QLYCLNASVGAPPDVLGPQGQNWGLTPMNPHVLKQQAYRPFIELVRANMSHCGALRLD
                     HIMSLLRLWWIPKGDSAVNGAYIRYPVDDLIAILALESQRHQCLIIGEDLGTVPKEIV
                     SKLKNAGILSYKIFYFEFDEQGKSRDLQHYPYQAMTTLSTHDLPTINGYWRGYDFELG
                     EKFGVYPNSNILAILKQDRVNAKAKILQRLQEHNVEIAPEISEQLNSPVNTAFIHCLQ
                     TYVANVSSGLFGFQPEDWLGMTEPVNIPGTSTQYANWRRRLSVNLEEIFTDKDIQALL
                     KEVNLIRKNQ"
     gene            complement(19461..21122)
                     /locus_tag="PXE44_000470"
     CDS             complement(19461..21122)
                     /locus_tag="PXE44_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDL5588013.1"
                     /translation="MSEIALTVSLLSLVAVLGLWIGHIKIRGVGLGIGGVLFGGLFVS
                     HFMAQFDVKLDAHTLHFIQEFGLILFVYSIGIQVGPGFFASLKHSGLKLNGFAVLIVL
                     ISGILVILIHKFFNVPLPVILGIFSGAVTNTPSLGAGQQVLAELSAESVTEIMGMSYA
                     IAYPFGIIGILLSMWLIRILFRINIEAEAEAFNKQQNRSKDQLSTLNVRLTNPNIHGL
                     TLKQIPDFNLHEVAYSRLKRGNELSIPRVDTILQIGDILHLVGEESSLRKMQIILGEA
                     VDVSVSTKGTIYRSERAVVTNEKVFGKQIRQLMLKGKYDVVISRLNRAGVELVPTGDM
                     TLQFGDVLNLVGRQADIEMVMTIIGNAHSKLQQVQMLPIFIGIGLGVLLGSVPIYLPG
                     FPVALKLGLAGGPLVVALILARIGSFSKLYWFMPPSANLALREIGIVLFLAVVGLKSG
                     GRFLETLLSSEGVEWICYGAIITFVPLMIAGLIARMYNKVNYLTICGLLAGSMTDPPA
                     LAFANEIKEGNGAAALSYATVYPLVMFLRIMLPQVLAIMLWVSGG"
     gene            21340..22716
                     /locus_tag="PXE44_000471"
     CDS             21340..22716
                     /locus_tag="PXE44_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDL5588014.1"
                     /translation="MLSNEVFISIIVLLILSLLRINVVIALIISALTCGLIGNLGVEG
                     AGFTEALEKTIKSFTGGLGGGAETAMNYAVLGAFAVALSKSGVTDLLAYKVIKSFGHS
                     PSGRSVTWFKYIVLFVLVAFAMSSQNLIPIHIAFIPILIPPLLSVMNKLQIDRRAVAC
                     ALTFGLTATYMLIPAGFGQIFIESILVKNINQAGEPFQLVTTTAEMTKAMLIPVSGMI
                     LGLLFAFFVSYRKPRVYIENMPEPTADDIEARVANVKPFHIGISIVAIIATCSVQLAT
                     NSTIIGGLVGLVIFALGGVFRLKESNDVFQAGLRLMAMIGFVMIAASGFAGVVNSTGG
                     VPALVESLRDVISSKEMAALLMLVVGLFITMGIGSSFSTVPIITSIYVPLCISFGFSP
                     LATMAIVGVAAALGDAGSPASDSTLGPTSGLNADGKHDHIWDSVVPTFIHFNIPLLVF
                     GWIAAMTL"
     gene            22814..23617
                     /gene="xthA"
                     /locus_tag="PXE44_000472"
     CDS             22814..23617
                     /gene="xthA"
                     /locus_tag="PXE44_000472"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL5588015.1"
                     /translation="MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPY
                     DLVNHLGYHVFHHGQKGHYGVALLTKKEPIAVRKGFPTDVLDAQKRMIMADIETDFGV
                     LTVLNGYFPQGENRSHETKFPAKQKFYADLQNYLESELKPENPIIIMGDMNISPTDLD
                     IGIGEPNRKRWLRDGKCSFLPEEREWLDRLYSYGLVDTFRAGNPDVNDKFSWFDYRSK
                     GFDDNRGLRIDLILASKSLAERCVETGIDLDIRAMEKPSDHSPVWATFK"
     gene            23771..24355
                     /gene="lptC"
                     /locus_tag="PXE44_000473"
     CDS             23771..24355
                     /gene="lptC"
                     /locus_tag="PXE44_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDL5588016.1"
                     /translation="MNKRLTVILLIIVAVLGGWYYSLQEKENTGLDQLIKKEGQPEYV
                     GNKMSTTVYDLKGNPQYFAQATEIKRYESTERTEFFKPFIELFAKESTQKQWKVSADY
                     AEITKEKMLNLKGNIKLVALDPASRLQQITTDTLTVDLNTQDIFTESTVKSIGSGFTT
                     TGVGLKGNLKQQVATLQKDVKSYIEPTIIQETKE"
     gene            24379..24885
                     /gene="lptA"
                     /locus_tag="PXE44_000474"
     CDS             24379..24885
                     /gene="lptA"
                     /locus_tag="PXE44_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HDL5588017.1"
                     /translation="MKLMFKSILVSVLLGSSFSAYALKGDTDQPINIDSGSQSLDMTN
                     NIVTFSDNVVITQGSIKVTAANVKITRQEGKKETIDATGSPVTFQQTLDNGKPVNGKG
                     NSVHYDLNSEFLTLIGNAELKQQGSFIKANKITYDVKKQQLKATSGGKTRVKTVLIPN
                     ELQDNKKK"
     gene            24897..25622
                     /gene="lptB"
                     /locus_tag="PXE44_000475"
     CDS             24897..25622
                     /gene="lptB"
                     /locus_tag="PXE44_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005727816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDL5588018.1"
                     /translation="MSVLYAEHLAKSYKQRQVVKDVSLNVKSGEIVGLLGPNGAGKTT
                     TFYMVVGLVRHDHGTIRIDDQDISTLPMHDRAKQGIGYLPQEASIFRRLSVYDNLMSV
                     LQVRKNINDKQRKERAEELITEFHIEHIRNSLGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKKIIVNLKERGLGVLITDHNVRETLDVCERAYIVGSGEMIAS
                     GTPQQVLENPDVKRVYLGDQFKL"
     gene            25664..26176
                     /locus_tag="PXE44_000476"
     CDS             25664..26176
                     /locus_tag="PXE44_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5588019.1"
                     /translation="MTKLTEFLSPENVRQGVLISSKKRALELVGKIVAESINQTEQQS
                     EALCPIECFGNLFKREKLGTTGLNQGIALPHAKLPACGDIELTKPIAVFLQLEEAIDY
                     EAQDNKEVDLIYAMMFPENSCEQYKSCLPAIAQKLTDKSLLKQLRAAESAEDIWQILV
                     YADNHLKDEE"
     gene            26189..27052
                     /gene="rapZ"
                     /locus_tag="PXE44_000477"
     CDS             26189..27052
                     /gene="rapZ"
                     /locus_tag="PXE44_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL5588020.1"
                     /translation="MELIIISGRSGSGKSVALRALEDAGYYCVDNIPLPLIPTLTYYL
                     TQENRSAVISLDIRNLPNTPNAIEELLTQLEPLNAKLIFLDCERNTLIRRYSDSRRIH
                     PLSTQDDLSLEGALDLEQKLLEPLVQNANYIIDTSHLSSHELAENLRQILQGSTEKEL
                     KIIFQSFGFKYGLPADSDYVFDVRFLPNPHWNPELRPMTGLEQPVIDFLERQTEVHNF
                     IYQTRNYLEMWLPMLEQNNRSYLTIAIGCTGGKHRSIFIAEQLAKYFQAKGKNVQVRH
                     KSLEKHHKKTS"
     gene            27135..28319
                     /locus_tag="PXE44_000478"
     CDS             27135..28319
                     /locus_tag="PXE44_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL5588021.1"
                     /translation="MKSADIVIVGGGMVGLALAGLLKDVDCQIKIIEKNTPKFDADTI
                     YHRVSAINASSQKILEQIGAFQHIPAQRLSPYTEMKVWEKDSFANIHFDNNDNEIKQL
                     GLSQLGFILENAVIQHALWQQVSQQANVEYIIAAPQTLGVSENGAFLTLDNGEMLSAK
                     LVVGADGANSWVRNQMKMPLTSKDYQHTALVCNVKTTEKHGKTARQIFAEDSIFAFLP
                     MADEHLCSIVWSLPPNKANELKNASEAEFNKALTIAFDNQLGLCEVQSLREIYPLAAR
                     FCRDFAQPRIALIGDAAHTIHPLAGLGVNLGFADARTLANEIQKQLVNNQDIGEYRHL
                     RQFERERKAQAMKLLATMEGLKQLFDGNHPLKKLFRGIGLSLTNQLPFVKKQLIQQAI
                     YL"
BASE COUNT         8567 a         5861 c         5570 g         8390 t
ORIGIN      
        1 ttagattatt tagcacaaaa acatcattaa tcttatttat attatacagg tgggaattct
       61 atgtcaggat tagttagtcc ggaattaatt aaaacagtcg cattacttgc aaccagcgtc
      121 actattgtac cgctatttaa acgccttggt ttaggaagcg tattgggtta tttagtcgca
      181 ggatgtttaa tcgggccatc gggcattgga ttgttccaag accctgccgc tgttgtgcat
      241 atggcagaac ttggcgtggt tatgtttttg tttattattg gtttggaaat gcacccggaa
      301 ctgctttggg cgatgcgtaa agccatcttc gggcgaggtt tcttacaagt tgcgatttgc
      361 ggtactttat taaccttagc cggcatttac ttattaggtt tttccaaaga agtggcattt
      421 attgccggta tgggttttac gctatcttct actgccattg taatgcaagt gctggaagat
      481 aaagggataa gttccacgcc aaaagggcaa cgtattgttg caactttact atttgaagat
      541 ttagcgattg tgccattatt agcctcggtt gcctttcttg ctcctaacca agtagatgct
      601 gaatccagta ctaattggtc tgctattggt cttagcctag ttgccgttct tattttggtt
      661 gcgagtggaa aatggctgat gaaccctatt ttccgtgtta tttctaaagc caaagtacga
      721 gaaatgatga ctgccgctgc cttacttgtg gtattaggct ccgctctttt aatggaaatc
      781 agtgggcttt caatggcaat gggggcattc cttgccggag taatgctttc ggagtcttct
      841 ttccgccatc aactagaagc tgatattgaa cccttccgtg gtttactttt aggcttattc
      901 tttatgggcg taggaatgtc attagacctc agcctcgtcc tcaataattg gctatggtta
      961 ttggtgattg tagcagttta tgttatcggt aaaggccttg gaatcttcat tgctgcctct
     1021 atcacccgat taacactgaa tgaatctatt gtgcgaacta ccattatggc tcacggtggt
     1081 gaatttgcct ttgtgctatt ctcagccgcc gcaaccgctg ggattttaac cccagatact
     1141 cacgctacct taaccgctgc ggtaattgtc tcaatgttgc tttccccact tctcatttta
     1201 atcaagcaag gagtgagaaa gaaaaaagcg aaaaatgaat ccactaatat gacaggggtt
     1261 gaattaccgg aagatttaga agccaatgta ttagtcatcg gttttggacg ctttaaccaa
     1321 atagtctgcc aagccttatt agccaaagga ctaagtgtat cggttattga ttcaaatact
     1381 gatcatattc gtgctgctgc tcgttttggt tttaaacttt actatggtga tggagcgaga
     1441 ctagatgtac ttagggcatc tggcatcgcc aatgcaaact gcgtaattgt tggcgtaggc
     1501 aatccggaaa gaacggagaa aatcgtagag ctgattaaat ctgaataccc cttagtacct
     1561 gtatttgcga gaacctatga tagacaaagt gcggttgatt tagtcaaaaa gcaccacgtt
     1621 gattatcaca ttcgagaaac ctttgaatca gccctcgcat taagccaagc cgccattgaa
     1681 cgcttaggtt ctagcgagga agaggcaaaa caaattatca gctatgttcg ccgattagat
     1741 gcagaacgct ttaatgagga attattacac ggtttctccg cagaagttat ccgcaaatat
     1801 tgggtaccaa caccttgggt aaatccacaa cacgaaggta gtgcacttaa tgaagaaacg
     1861 gctgatattt tagaagagct tggtgagaca gtagaagtta taaaagtgga tatcactgat
     1921 aaaaattaaa gaatttatac tttgaatcct tattcaagta taagccttcc tatacatatc
     1981 gcataatcac tcgtaaaaaa gcaccctatc ggcataatct gatagggtgt taatggtaat
     2041 aaaccatcgc acgatgacaa ggagaatatc tatgaaaaaa acaataaatc aatttgaact
     2101 tacatttttt aagaaaattt ggcgtggaga agtcagccct aatttttctg cctcgttaac
     2161 caaattcatc gctttattaa tatcttttgc tttaatcgct ttcattaccg cctgattaaa
     2221 ataggcttcc gtatctttat ctactgctgt ttttactgca actggatttt cttttatcgg
     2281 caacggtata gccgttttag ctgccggcgt tgctgctttg tttgagctga aaatttcagt
     2341 cggtaatcca ataaatttag tgccgttcga gccggtaacg ttgatgtgaa tttgcccttt
     2401 taaactgtgt gctacttcaa tatcgttaat tgctggtggt tgtttaccgg ttgcttttgc
     2461 ataggttttt gccggatgcg gcatcatcgt ggttttggct aaatcttgct gcgtggtata
     2521 aatgagcaag taaatataat cttggttcat tgccggcgtt aaattaagtt ctgcccctaa
     2581 acggctacct ttgagtcctc gctcttccaa taatttaaac tcagaagagg ggtaagttga
     2641 tgccacatta aattgactat ccaacacgac tgccgtgggt acaaaaacat ttttgtccat
     2701 caccgggctt tcgatttcaa tttccaatgt gccttgattt gccggaatac gataagctgc
     2761 aaccgggctg gcaataccgg caaacgctgt ggtaaatgct tgtttgtgtt gctcgcttag
     2821 atctgttttt acggtttgtg aaaatggcac atcttgccat tgaatttgag ctaattcagc
     2881 cgagttaata tgaatagact tacctgccga gccatttgct atactcggag acaccaccaa
     2941 aagatgggcg aagaatattg ctgtcgaaag aagcgttttt ttcatattaa cccctaaaaa
     3001 tcgtgaaata ggataaagtg ctgaaatagt gggtaggtaa tcctacccac acaaaggttt
     3061 gatgattacc accaagcttc aaattgaaca ccggtgataa actcgccgtc tttggcttta
     3121 tagccggcat cggtacgagc tttcgtgttg aatttatcat tccaacgtgc ataagtacca
     3181 aacacacgga ttgcagggcg agcccaaatg ctattaccag cctgccattg ttgtgcaacg
     3241 gtgtatttta ccaagtcatt tttcttgcct gttgcttgat ctttaatgcg atcataacca
     3301 acttctaata atgtactcat tgtatcattc catttgtaca ttgggcgaat acctgctgag
     3361 taccaagttt taccttgttt attatctagt tttgtttttt cgtagattaa ggcatacatt
     3421 acttcaactt tatcactcgc ttgcactacc ccttggttga ttaaacgaag catattacct
     3481 ttattatctg ctttcgaacc ctgtgcgtgc ccattattcc aagaagtcat agagtcggtt
     3541 gcatattgtg cagtaaattt attgaaacca ccaaagaagt taccttgagt gtattccgct
     3601 gttaccatat aaccgttttt ggttgctcct ttttcaaggg ctgcaccttt cttagcatgt
     3661 gcattaccgt aatcaaagcc taactctaac gaaccatctt tccaaagctc aataccggct
     3721 aaccgaacat caaaaatatc gttgtaaaca tcggctttat tacgctgctt atctaattta
     3781 attttacctg tgctaggatc taatttataa actgtagtat atccaaaagt attcgcccct
     3841 ccacgctctg tatcacgagt aaccgctaaa gagagttttc cgaagcctaa atcaatattt
     3901 tcaacccctg cacccggacc tgaaatatcc cagtaataga agtcattcat atgtacatca
     3961 tggcgttggt aaaaacgttt accagcccat aaggtggcac ctggtaaact atctgcaaag
     4021 tttttgaact gtacatttaa ttcacgtagt gctggacttg ttggtgtcca atcattattg
     4081 tgaagcgtac cgccgtaagc aaggtttgta tcaaagtaga tagatttctc accacttttg
     4141 aataattcct gacctaattt caattctgca taggtatcag attcgttacc taaacggtat
     4201 tttgaaccac caccatttac cgtgaatgcg gattgctccc ccccccccga tgtccaaccg
     4261 atacctgaac gtgcatatcc gtggaaatca accgctgatg cactggctgc gaacattgca
     4321 ccactgattg caactgctag gactgttttt ttcataatag aacctctctt aagatttgat
     4381 tttacttagg ttaattatta attgattaga agattgaaaa taccctctaa aatcagtaat
     4441 aaactgaaat tgttataccc ctttttcaac aaacaaacgt ttgcaagctg taccgtcttc
     4501 cttaaataaa tggcagcgtt ccggcaatat accgatattc agatcgtcgc cttcattaac
     4561 taataccaca tcgttttgac gataaattaa ggttggttgt ttgatttcag ggatttcaag
     4621 gtggatttgt gtttcatttc cgagcaattc cacaacttgc acaataccgt gtaaggtcac
     4681 ttgggtttga tccgaaggaa ctaaatgttc cgggcggata cctaaagata agttatctcc
     4741 cacattcacc ccttcgccgg aaaccggaat ccaaaaatta tgatgattcg catcaggtaa
     4801 ctcaattttt acccgttccg gctctaccgc aatcactttt accggtaaaa agttcatttt
     4861 aggcgagccg ataaaccctg ctacaaagcg atttgccggg taatgataaa gttctaacgg
     4921 tttacctact tgagcaatac cgccggcatt taataccacg attttgtccg ctaatgtcat
     4981 tgcttcaact tgatcgtgcg taacataaat cattgtgcgg tttagttttt tatgtaattt
     5041 ggaaatttct acccgcattt gtacacgcaa tgcggcatca aggttggaaa gtggttcatc
     5101 gagtaaaaat acttccggtt gggaaaccag tgttctacca atagccaccc gttggcgttg
     5161 tccgccggaa agtgctttag gttttcgatc taataaatgg gcaagctgta agatttcggc
     5221 aacttggtta actcgttgat cccgctctgc tttacttaca cctgccagct ttaagccaaa
     5281 agacatattt tcggcaacgt ctaaatgtgg atagagagca taggattgga acaccatacc
     5341 gataccacgt tttgaaggct ctacttcatt catcagtttt ccaccgatat agagttctcc
     5401 tgtcgtaata tcttccaaac ctgctatcat tcgtaatagc gtagatttgc cacaacctga
     5461 cggtccaacg aaaacaacga attctccttc gttaatttct aaattaatat ccttagagat
     5521 atgaacatca ccatacgatt ttcccacgtt acgtaatgat acattcgcca taactaaacc
     5581 tcttttctgc ttttgcttgt tggttttatt taggctgaat agtcgccttt ttatctaaag
     5641 aagaaatcat cctaaaatac ttttttttag ggttagagcc atagccatag ccataattat
     5701 ttaaaaaacg ccaattcaga aaaattaaat aaattttgtg atcaacctct catttttaat
     5761 gcctattttt gtgaagcaga tcacaaaatg ataaaaaatg ttacttagat catactttta
     5821 gcttgggggg cgtagagcaa agggtgatct caaatagatt gtatagccca taatttgcca
     5881 taatttaata aatgggttgt ttcatatccg tttcttttat ttaacttcaa ggagtttttt
     5941 atgaaaaaca aatgtgtcaa attcacctta gctactcttg ctaccttagt tctttcagga
     6001 acagtaatgg caaaaatgac ggaaggtaaa ctcgttatct ggattaacgg tgataaaggg
     6061 tacaatgggc ttgccgaagt cggtaagaaa tttgaaaaag ataccggtgt tcaagtttta
     6121 gttgaacacc cggatagact tgaagaaaaa tttgcacagg ttgcctcaac tggtgacggt
     6181 ccggatatta tgttctgggc tcacgaccgc tttggtggtt atgcacaatc cggtttattg
     6241 gctgaagtga atgtaagcaa agaatttaaa gataaatttg tcgattttgc ttgggatgct
     6301 gaaacttaca acggcaaaat tatcggttat ccggttgcga ttgaagcgat ttcattaatt
     6361 tataataaag atttagtgaa agaagcacca aaatcttggg aagaaattat tgagttagat
     6421 aaaaaactca aaaaagaggg caaaaatgcc attatgtgga acttatccga gccctatttc
     6481 acgtggccga ttgcggcttc aaacggagct tatgcattca aatttgctaa cggcaaatac
     6541 gatccaaaag atattggtgt gaacaatgaa ggggcagtaa aagcactaca atttgtagtc
     6601 gatatggtga aaaataaaca tattagtgcg gatatggact atgccgttgc cgaagcctcc
     6661 tttaataaag gacaaagtgc cttaaccatc aatggtcctt ggtcttgggg caatattgat
     6721 aaaagcggta ttaaatatgg tgtaacttta ctcccaacat taaacggtca agcttctaaa
     6781 ccattcgtag gggtgttaag tgccggtgta aacagctcaa gcccaaataa agatttagcc
     6841 aaagaattct tagagaatta tttactcacc gacgacggtt tagatgcagt caataaagat
     6901 aaacctttag gtgcggtggc gttaaaatct taccaagaga aattagcggc tgatcctcgt
     6961 attgctgcaa cgatggcaaa tgcgaaaaac ggtgaaatta tgccaaatat tccgcaaatg
     7021 tcttctttct ggtatgccga aaaatctgct atcaataatg cagtgaccgg tcgtcaatcg
     7081 gtgaaagaag cattagatga tgcacattca cgcattcaaa aacaacaata attcttcatc
     7141 attctgagtg ggttattatg acccactcta tttttagatt caccctaaat acaataaaag
     7201 tacagtaaaa gtaccgtaac aaaatgcagt aaaaattcga ggtgctgtta tgctaactca
     7261 aactcaatcc aaatccactc attggctcaa atatctctta gccggattag tcttgctcct
     7321 tgacttctat cttgttgtct taatgtactc acaaggtgaa tatctttttg ctattttaac
     7381 gctgattatt ctaacttccg gtgtctatat ttttaccaat aaaaatacct atgcttggcg
     7441 gtatgtttac cctggcatta ccggaatggc aatttttatt ttgtttccgc tggttgcgac
     7501 tattgcaatt gcgtttacca actatagtgg ttctaatcaa ttatcctttg aacgagcggt
     7561 atccgtctta actgaacaac gctattttgc cggagataaa tatcaattca ccctttatcc
     7621 gcaagcagat aataaatatc aaattgcatt aaccaatccg acgacagagc aaacttttgt
     7681 gtcagagccg atttcattgg ctaccggaac gaatgtggtt gtaaccgagc aaaacagacc
     7741 aactggcgaa gtcgctcccc ttaaagtgat tacgcaaaat cgggccgcac ttcaatcaat
     7801 gaaagtggtt ttaccgaata ataatgaact gacaatgagc tcattacgtc aattctccga
     7861 gcaaaaacca cgctatcaat ttgatgaaga aaataaaata ttactcaata atgagactgg
     7921 caaacgttat caagcaaatg acgaaaccgg tttcttccaa tcgattgatg aaaacggtca
     7981 ttggcaaagc gaaacacttg aaccaggtta tactgtcaca tcaggcttta ataacttcat
     8041 taaaattttt accgatgaag gtattcaaaa accttttgtc caaatattta tttggacggt
     8101 gatcttctct ttattaaccg ttgtctttac cgttattctc ggtatggttc ttgcctgttt
     8161 ggtacaatgg gaggcattaa aaggtaaagc agtctatcgt gtcttattga ttttacctta
     8221 tgccgtacca tcatttattt cgattctgat ttttaaagga ctatttaacc aaagttttgg
     8281 tgagatcaat atgattttaa accaactatt cggcatcagc cctgaatggt ttaacgatcc
     8341 gctccttgcc aaagtgatga tcttaattgt caatacgtgg ttaggttatc cttatatgat
     8401 gattttatgt atggggttac tcaaagctat tccggctgat ttatatgaag cctccgcaat
     8461 ggacggtgct tccacttggc aaaacttcag taaaatcact ttcccattac tgttaaaacc
     8521 acttactccg ttaatgattg cttcgtttgc atttaacttt aataactttg tattaattca
     8581 actattaaca aacggtcgcc cggatatgat tggaacaacc actccagccg gttataccga
     8641 tttattagtc agctacacct atcgtattgc ctttgaagga agtggtacgc aagatttcgg
     8701 cttagcagcc gccatcgcaa ccattatttt cttattagtc ggtggcttgg cactcttaaa
     8761 tatcaaagca accaaaatgg aattataaaa gcgggaggga tttaacaatc ccgaccccaa
     8821 actaacttta ggagttatat atggcaattg ttcaatctaa atctgtgcgt tatcgggtat
     8881 gggcaaccca tttcatctta atctgtttcc ttgccttaat tattttccct ttattaatgg
     8941 tgattggcat ttcgttacga ccgggaaatc tggctatcgg tgatattatt ccaagccaaa
     9001 tttcgtggga acattggcaa gccgctcttg gttttgatgt tacacacgcc gatggtacag
     9061 tcacaccgcc gccattcccg gtgctacgct ggttatggaa ctccatcaaa gtggcaacaa
     9121 ttacctctat cggcattgta accttatcta cgacctgtgc ttatgcattt gcccgaatga
     9181 aatttaaagg caaaaaaacc attctacaag gtatgctgat tttccaaatg ttcccggcag
     9241 tgctttcctt agttgcctta tatgcgttgt ttgatcgtct tgggcagtat gtaccgtttc
     9301 ttggtttaaa cacacacggt ggtgttattt ttgcctactt aggcggaatt gccttacacg
     9361 tttggacaat caaaggctac tttgaaacga ttgatggttc attggaagaa gccgcttccc
     9421 ttgatggtgc aaccccgtgg caagcgttcc gattaatctt attaccgctt tcagtgccga
     9481 ttttggcggt agttttcatt ctttcattta ttgccgccat taccgaagta ccggttgcct
     9541 cactcctatt gcgtgatgta aacagctaca ccctagccgt tggtatgcaa caatacctct
     9601 atccgcaaaa ctatttatgg ggtgacttcg cagcagcggc ggtcttatcc gcaattccga
     9661 ttacgctggt cttcttattg gcacaacgct ggttaatcgg tggtttaact gccggcggcg
     9721 tgaaaggcta gtttgcaaaa aattctctaa aaacgaccgc ttgtcgagct gatactgcgg
     9781 tcgtcaatct ttattagggc tgtctatgaa aaatgcgtta ccttatctct gcttattcta
     9841 tacctcaagc ctttttgccc aatcttggca acattcccat ttttctgatt ttaacgatga
     9901 tctcgaaaaa catttttttc aatcccaagt tcaattggaa aaagggagct atccgcttag
     9961 gttcaaattt ggcgaacaat gttatcaacc ccaacaagcg ataaaattaa atcaatcggt
    10021 tgcgttagtc ccttgtataa atgaagctcc acaactgcgg ttgtttcgtc aaggcaacta
    10081 tctggcacaa attgatctgc gaagcggcac tccgacaccc acaatcagcg tagaacagca
    10141 gctacagcac aatgaagcga tgtttcaaag ttgcccgaat tgggataaaa aaccgattga
    10201 aattgatgtt tcttccactt ttgccgaagg cgaatcggtt agcgattttt attcaggcaa
    10261 caccgctatc gttaaaaacg gcaaagtcac cttaatgccc aatgaaaacg caagcggtct
    10321 gattttgctg gaaaaaagca ataccgaaaa tacggcaacg tttgattgga aaaatgccac
    10381 cgtctatttt gtgctgaccg accgctttta taacggcgat ccgaccaatg accacagcta
    10441 ccaccgccaa aaagacggaa tgcaagaaat cggcaccttt cacggtggtg atttaaaagg
    10501 attaaccgaa aagctcgatt acctacaaca acttggggta aacgtattgt ggattagctc
    10561 tccgttagaa caaatgcacg gctgggttgg cggcggtgat aagggggatt ttccgcatta
    10621 tggctatcac ggttactatc atttagattg gacgaaatta gatgccaata tgggtactga
    10681 agacgattta agtcgctttg ttcaacaagc ccaccagcgt ggtattcggg tgctatttga
    10741 tgtggtgatg aaccataccg gctacgcgac gcttgccgat atgcaggagt ttaactttgg
    10801 cggattttac ttaacaccgg acgaaattag ccgcacatta ggcgaaaaat ggacacactg
    10861 gaagccgaaa tcaggacaaa attggcacag tttcaatgat ttcattcgct ttaacgataa
    10921 tcaggcttgg caaaattggt ggggaaaaga atgggttaga gctgatattg ccgattacga
    10981 cagccctaaa tttgatgatt taaaaatgtc gctcgctgcc cttcctgatc taaaaaccga
    11041 gagcgaacaa gcggtcaaat tacctgaatt ttttgcaaat aaaaatacca atgccaaaac
    11101 attaccaaac gccaaagtgc gtgattattt aattggttgg ttatccgatt gggtcagaaa
    11161 atatggcatt gacggttttc gggttgatac cgccaaacac gttgaaaaat ccacgtggct
    11221 ggtattaaaa caatcggcac aacaagcctt aaacgaatgg caaaaagcca atccgaacgc
    11281 cggttttaac gatcgctttt ggatgacagg agaagcgtgg ggacacggcg tgttcaaaag
    11341 tgactattat caaaacggtt ttgatgcaat gatcaatttc gattttcaag atcaggctaa
    11401 acaagggtta aattgctttg cccatattga gccgatttat gccgaaatga atcgcaaatt
    11461 aagcgacttc aatgtgttga gctatttatc ttcacacgat actcgattat ttttccattc
    11521 ggatagtgaa caaaatatcg aaaaacacaa aaccgccgca aatttgctgt tgctttcacc
    11581 cggtgcagtg caaatctatt atggtgatga aagtgggcgg acatttggtg cgaccggttc
    11641 agacctgata caaggcaccc ggtcggatat gaattggcaa gagttgcagc aagatcctca
    11701 gaaacaggca ttgcatcaac attggcaaaa attgacacaa ttccgccaac gccatccggc
    11761 tattggcgca ggtgtccatc aactcatcaa aaatgatagc tactttgcat ttaagcgtgt
    11821 gttgaatgaa gataaagtga tggtggtttg ggccggaaat taaatctact ttagcttcaa
    11881 gttttctctc ttctggggaa aacttgaagc cggttcgggg aaacaagttg caaaaaatta
    11941 ctaaaaatag accgcttgtt ccccatgagc ttgtgatcaa tgatacccca tcaacatcag
    12001 cagttctttc gtatagctga ttgcttcgtt acgattcgtc acaccgattt tctgataaag
    12061 atttcggata tgggttttaa tcgtggttgc agccacttgc aattcatctg aaatttgttc
    12121 attgctatat ccggagtaaa tcaaacccaa tacttgccat tcccgctgag tgagcggact
    12181 aatttttaag agttccggca ctttcggatt tttcaataat tgattcacaa agttttcatc
    12241 aaaatgggca aatttttggc gataaaattg attgatgttg cgtaaaataa actgtgcttt
    12301 gtgcaatacc agttcatcta gcacattaag ttgcaataaa tggcggatct gctgtgccat
    12361 tacatcccct tcaatcacaa acgcactgat aaaattcgtt tggcgggtga gttttagtgc
    12421 ctgaattaaa tcttgctgag ccaattcttt ctcgccttgt aaaaaataaa gccggttacg
    12481 aacaattaat gcgcgattta aatcgctaat cagagaaaat ttttcagccg tttcaattaa
    12541 attatctaaa atggctttcg cctcgtgata ttcctcaagc aagattcgag accgtgcaat
    12601 atttcgccat tgcacttggg taaaatgatt tttatctgaa accggtgagg ggttttgaat
    12661 taaccagttc ctcaccgcag aaacgtcatt ggtcatctgc caataaaata tacgtacctg
    12721 atcggcgttg gcaatccaat cgtggtgata agaatgagaa tgttctaatt gttcgacctc
    12781 gcctaataat cgggcagtgt tatccaaatt gcctcgagcc aacgagatct tcgtgagtaa
    12841 agttaagcat tgtaacttat cttcaaattt agataaggca ttcattccgg caactgccat
    12901 tgattcagct ttatctaagt tataccattc ccataaaatc ttgcctttag aacgtagcaa
    12961 aaattcatac atcggcactt tttgtagatg gttttctttc acaaactcgc ttgctttatc
    13021 taacatttca taggctgctt gtgaaaaccc ttgggcaagc aaaatttcag attgttgaag
    13081 ctgtgaccac aaaatattgt ggtaagtatg atgctgacgt gccatacgct ctgctttttg
    13141 caacattgct aatgcttctg ccagattgcc gtggcaatga tgagcttcgc caataataga
    13201 cgttgcaaca atatgagcat aataggcatt ttctgaaaga tcgtttaacg cttcagacgc
    13261 taattgaagt gcggtattct catcaccgga attgatagca acttgtgccc gtagcacatt
    13321 aaattccgca ttggcagttt tgctcaattc aattttattt tcagttaatg cacgagaaaa
    13381 atccgctaaa ataccaccta cttcaatatg gcggtgctgg ctttgtacca accaggcttt
    13441 gagtaacacc aaattagtat gttcggttaa atgggcgtaa tctaagcaat ttaagctctc
    13501 ttccaataat ttgagttctc cttgatgaaa caccgtccaa gcgtgtttgc tcaaaatatt
    13561 gagtagtaat ccggtatccg ataactgcat tgcgtgatgt aaggcttctg taacatagcc
    13621 taattgtaac cacgcattcg ccgccttttg atgcagttgc ggtaattcag catataactc
    13681 attttggcaa cataaattta aaaaggaagc aaaaagcgga tggaatttcc accagctatc
    13741 atcactactc tgccacttac tgtttgccat ttgttgtaaa aacaaacctt gcttttcgag
    13801 agactctaat ttggtgcgac tgttttgctc ccctgtaaca gcctgcacta aagactcatt
    13861 cattgaatgc aaaaccgaac aacgcaaaat aaacaaacga gtctcttcat cgactttatt
    13921 aagcacttca tcggctaaat attcgttaat atgaaaatgg tttgatttgg ctaaacgttt
    13981 ggcaatatct tgcagtggta cttgtagcgt gtgtgattta ttttttgcaa aaaggctaat
    14041 tagctgaagg gctgtgggcc acccttcaac ttcgttacac agtgcgataa catcttgctg
    14101 atttaactcc gaacctaatc ttgcttgaaa aaaagcggta ctttcttggt gatcaaacat
    14161 taactggtta atatcaattt ccaataattg ttcttgcact cttaaactgg caatacttaa
    14221 cgggggaatt gatcgagaaa tcaagataag cgtcatatta gacggttgat gtttaatcca
    14281 atattttaat gcttcgtgaa tttcatcatt ttcaataata tgataatcat caatagcaag
    14341 ataaaaatgc tctgaaaatg ccgcggcttt aatcaataat tgattaaata aagctaataa
    14401 attagcttta cgattttcct ctaacaatac gtctatttct tcattgattg ctgaatgcaa
    14461 tgccgcacta aaataagtgg caaaacgatc tgaattatta tcgccttcat ctaacgcata
    14521 ccagccgata ttctttttat tttcaatcca ttgggaaacc agagtggttt tgccataacc
    14581 tgccggtgca tttatcaata cgaccggata actgcaagat ttgtctaatt cttgaattaa
    14641 acgttttcga ggaatgctgt tttgtaatcg aaaagaacaa acaagttttg atggtattaa
    14701 cataataaat tcaattttct ctcataacaa aatagggatg atatatccac catttgtgtt
    14761 ctcactactt ccttataatc ataccataca agatgattat tgggatgatt tgattttatt
    14821 tggattttac ataatagcga cattcatctt taaccggggg tttcctatga catttaattc
    14881 tattgtagaa aaatactgtc gctactttga tgtgacagat ccaaaacact ttaccttaca
    14941 gcagtggtat caagttgttg ctgaaggttc ccttgagcta atttatagcc agcctgcaac
    15001 caaaccgaca gaatcacgcc acgtgaacta cctctcaatg gagttcttaa tcggtcggtt
    15061 aaccggcaat aatttaatga atttaggtta ttacgaacaa attcgtgact atcttaaaca
    15121 atatcaagtt gaattagtag atgttttaga acaagagcgt gatccggcat tgggtaatgg
    15181 cggattaggt cgtcttgctg cctgcttttt agattcgatg gcaactgtcg gacaaaatgc
    15241 aaccggctat gggcttcatt atcaatatgg tctgtttaaa caaagcttca aagagggaat
    15301 gcagaaagaa gcagcggata cttgggctcg tgacagctac ccgtggcatc gttttaatcc
    15361 gtctaaaacc cgccatatcg ggtttggtgg aaaaatcaaa catatccaag ccgatcaata
    15421 tgaatggcag cctaaattga ccattcaagg caaagccttt gacttgccga ttgtaggtta
    15481 taaaaacaac atcattcaac cattacgttt atggcaagca gatagtgacc aatcttttga
    15541 ttttgatgcc tttaatgagg gtaaattttt aagtgccgat aaaacgattg tcaatgcagc
    15601 cgcactaact caagtgctgt atccaaacga caaccatcaa gccggacaaa aattacgctt
    15661 aatgcagcaa tacttccatt gtgcctgctc ggttgccgat attttagatc gtcacattag
    15721 cgaaggttat tcgttagaag atttagcaaa acgccaagtt attcaactca atgataccca
    15781 tccaacgctc gctatacctg aattgatgcg tgtactgtta gatgaacatc aattcacttg
    15841 ggagcaggct tgggatattt gcagcaatac ctttgcttac actaaccata ccttattacc
    15901 tgaagcactt gaacaatggg atcaacgcct atttaagcag ctattacctc gccattatca
    15961 aattgtggaa aaaatcaatg atattttcca cgacaaagta caggcagaat ttggtgatga
    16021 tgcaaaaatt tgggaaaaat tagccgtgct atttgattac cgtgtgcgta tggcaaacct
    16081 ctgcgttgtc acctgcttcc gtgtaaacgg cgttgcggaa attcactccg atctcttagt
    16141 gaccgatcta ttcccggaat accacaaatt attcccaaca aaattctgca atgttactaa
    16201 cggcatcaca cctcgccgct ggattcgaca agctaatcca caattaagtg atttattgga
    16261 tcgtacctta aaacaagatt ggacacacaa tctggaatta ttaaaaggcg ttgaacaata
    16321 tgcggatgac accggtttcc gtgaagaata tcaacgcatt aaacgcaata acaaagtggt
    16381 tttagccaat gaaatccata aaacattagg gttaaatgtc aatcctaatg cgatttttga
    16441 tgtgcaaatt aagcgtttcc acgaatataa acgccaacat ttgaatttgt taaacattat
    16501 tgcgacctat caatcgctaa aagctaaccc gaaccaagac tacacaccac gtctattcct
    16561 attctcgggc aaagctgcac cgggttatta tttagcaaaa aatattattc acgctattaa
    16621 taatgtggca gatattatta ataacgacaa gcaggtaaat gatcgactac aagtggcatt
    16681 cttaccggat tatcgagtca gtcttgcgga aaaaattatt ccagcagctg atgtatcaga
    16741 acagatttca atggcaggta aagaagcctc cggtaccggt aatatgaaat tggcactcaa
    16801 cggcgcatta accttaggta cgctagacgg tgccaatgtt gaaattgctg atatggtcgg
    16861 tgaggagaat gtctttatct tcggtcacac cgtagaaagc gtgagggaat tattagcaaa
    16921 aggctaccaa ccgaaagcct actataacca agatccgatt ctgaaaaatg cggttgattt
    16981 cttagccaca ggaaaagcct ctaacggaga taaagaggca ttcaagctga tgttagatag
    17041 cctgttggaa cgagatcctt tcttagtctt tgccgacttt gatagctatc gccaagcaca
    17101 agagaaaatt ggtgtagcct atttagatca agaaacttgg ctgcgtagtg caattttaaa
    17161 tactgctcgt ttgggtgcat ttagctcaga ccgctcaatt aaagattatc aacaacgtat
    17221 ttggttgaat agataataaa ttcgggcgac ttctacaata aaggaagtcg cctattttct
    17281 gctgtatttc atatgtattt atatatggag agaaatgatg aaaataactg ccaaacaata
    17341 ttatcgggct ggaattatgc cctatttttt cgatgagcgt ggtgtaaaaa aaacagcatc
    17401 tgaaaaaata aaacattcgt tattcaaagc ctttaacggc aatactcaag caagagaaac
    17461 acctatccct acggtcaaca ttttttacca gcaccaaccc tgttttttac cttttcattt
    17521 agcggataaa aaacatccgt taaccggtac ttggcagcta caactagaaa attcaaacca
    17581 aattatttcc ggcaaaatta aagcaagggg aattacatta cctcaagatt tgcccttagg
    17641 ttatcaccaa ttacagctaa aaacggtaga taaaaccttt aattgcacca ttattatcgc
    17701 tccggaacgc tgctaccaac cacaagccgt cattgaacaa aaaagattgt ggggaacctt
    17761 cctacaacta tacaccttaa aatcagccca caactggggg attggcgatt ttggcgattt
    17821 aaaagacttc attcaacaga ttgcccctta ccaagcagat tttatcggct tgaacccgat
    17881 tcactcactt tttccggcaa acccgaattc tgccagccca tacagtccat cctctcgccg
    17941 atggctgaat attgcttaca ttgatatcaa tcagctagcg gaatttcaac aaagtgatga
    18001 agcacaaact tggtttcatt ctgctgaaat ccaacaacaa ttatcagaat tacgcactca
    18061 ggattggttg aattacgagc aaatcattcc gttgaaattg aaagggctac gctttgcttt
    18121 cgcaaaattt cagcaaaata tgaccgcttg cgctgcccct gcttttatcg aatttgtaca
    18181 aaaaggtggt gaaagcttac aagttcaagc cacatttgat gcccttcatc agcatctatc
    18241 ggcacaattt gccgaccaat ggggctggga tttctgggag aaagcctacc aagactacca
    18301 ctccgcagcc gtgcagcaat ttagagaaac gcataaaacc gacatagaat tttacctttg
    18361 gttacaattc gttgccgatc aacagctagc agaatgtgat gctatctgcc gtcagcaaaa
    18421 tatgcgtatt ggtatgtatc gcgatctcgc cgttggcgtc agcccgaatg gtgcagaaac
    18481 gtggaatgac aaacaacttt attgcttaaa tgcctctgtc ggcgctccgc ctgatgtatt
    18541 aggtccgcaa ggacaaaact ggggcttaac cccaatgaat ccgcatgtat taaagcaaca
    18601 agcctaccgt ccgtttattg agttagtcag agctaatatg agtcattgcg gtgcattacg
    18661 ccttgaccat attatgtctt tactccgttt atggtggatc cccaaaggcg atagtgcggt
    18721 taatggtgct tatattcgtt atccagttga tgatttaatc gcaattttgg cacttgaaag
    18781 ccaacgccac caatgcctaa ttatcggtga agatttaggt actgtgccaa aagaaattgt
    18841 gagcaagctc aaaaatgcgg ggattttatc ttacaaaatt ttctattttg aatttgatga
    18901 acaagggaag agtcgagact tacagcatta cccttaccaa gcaatgacca cattaagcac
    18961 ccacgattta ccgactatca atggttattg gcgtggctat gattttgaat taggcgaaaa
    19021 attcggcgtc tatcctaatt cgaatatttt ggcaatcctt aagcaagatc gagtcaatgc
    19081 caaagccaaa atcttacaac gcttacaaga acataacgta gagatcgcac ctgaaattag
    19141 cgagcaactt aattctccgg tgaataccgc atttattcac tgtttacaaa cctatgttgc
    19201 aaatgtaagc agcggattat tcggcttcca acccgaagac tggttgggta tgactgagcc
    19261 ggttaacatt ccgggaacca gtacacaata tgcaaactgg cgacgtcggc ttagtgttaa
    19321 cctcgaggag atcttcacag ataaagatat tcaagcctta ttaaaagaag ttaatttaat
    19381 ccgtaaaaat caataaaagt tagataagaa aaaaggcgac tttccgtcgc cttttgggct
    19441 tatatgcttg atgtctatct ttatccacct gaaacccaca acataatcgc tagcacttga
    19501 ggtagcataa tacgcaaaaa cattactagt gggtaaacag ttgcatagga taacgctgca
    19561 gcgccatttc cctctttaat ttcattggca aaagcaagcg ctggaggatc agtcattgag
    19621 ccggcaagta atccacaaat cgttaaatag tttactttgt tatacattct ggcaatcaat
    19681 cctgctatca tcaatggaac aaaagtaata atcgccccat agcatatcca ctcaacgccc
    19741 tcactgctta atagcgtttc gagaaatcta ccgccagatt ttaaaccaac taccgctaaa
    19801 aacagtacaa taccgatttc acgcaatgca agattggcac ttggtggcat aaaccagtag
    19861 agtttactga aactaccaat tcttgcaaga ataagagcaa ccaccagtgg tccaccagcc
    19921 aagcccagct ttaacgcaac agggaaaccc ggtaaataaa tcggtacaga accaagtaat
    19981 actcccaaac cgatcccgat aaatattggc aacatttgga cttgttgcaa ctttgagtgc
    20041 gcattgccga taatcgtcat taccatttca atatcagctt gccgccctac taaattgaga
    20101 acatcaccaa attgtaacgt catatcacct gttggtacta attctacacc ggctcgattt
    20161 aaacgagaaa tgacaacatc gtatttgcct ttaagcatta gctgacgaat ttgtttcccg
    20221 aagacttttt cattcgtcac tacagcacgt tcagaacggt aaatcgtgcc tttggttgag
    20281 acagatacat ctactgcctc acctaaaata atctgcattt ttcgtaatga ggattcttca
    20341 cctactaagt gtaaaatatc tcctatctgt agaatcgtat ctacacgtgg aatagaaagc
    20401 tcattccccc gttttaagcg tgaataagct acttcgtgca aattaaaatc aggaatttgt
    20461 ttcaacgtta aaccgtgaat atttggatta gtcagacgga catttaaggt actaagttga
    20521 tctttacttc tattttgctg tttgttaaaa gcctctgctt ccgcctcaat attgattcga
    20581 aataaaatcc gaatcagcca cattgaaagt agaattccga taatgccgaa cgggtaagca
    20641 atcgcataac tcattcccat aatttcagtt acactctcag cgcttaactc agccaatacc
    20701 tgttgacctg ctcctaaaga aggggtattg gttacagccc cggagaaaat gcctaaaatc
    20761 acaggcaaag gaacattgaa gaatttatga ataagaataa ctaaaatacc gctaatcagt
    20821 acaatcagta ccgcaaaacc attgagtttt aagccagaat gttttagtga ggcaaagaat
    20881 ccaggtccta cttgaattcc aattgaataa acaaataaaa tcagcccgaa ctcttgaata
    20941 aaatgaagcg tatgggcatc gagcttcaca tcgaattgcg ccataaaatg ggagacaaac
    21001 agcccaccaa ataacactcc ccctatacct agccccacgc ctctgatttt aatatggcca
    21061 atccataagc ctagaacggc cactaaagat aataaactga cagtaagtgc aatttcgctc
    21121 attacagcct ctctttaata tgaaatacct caaaaatggt atccaattct accaaatctc
    21181 aaaaacgaaa aatgagatct atgtcaaaaa aataaaatat tgatagtatt actccaagat
    21241 gaacttcaaa aaagttcctg aatttaaaaa aaattctcga caatactttt ttttacagta
    21301 aattaaacaa ttatttaaat aacctaagga tcaaacacaa tgttatcaaa cgaagtcttt
    21361 atttcgatta tcgtgctgtt aatcctaagc ttgcttagaa ttaatgtagt aatcgcactt
    21421 attatttctg cccttacttg cggtctaatc ggtaatcttg gtgtagaagg ggctggattt
    21481 accgaagcat tggaaaaaac aataaaaagt tttactggtg gtttaggtgg gggtgcggaa
    21541 actgctatga actatgccgt tcttggtgcg tttgccgtag ctctatcaaa atcaggcgtg
    21601 accgatttat tagcctataa agtgatcaaa tcctttggtc acagcccgtc aggtcgctcc
    21661 gttacttggt ttaaatacat tgtattattt gtgttagtgg catttgcaat gtcatctcaa
    21721 aacctaattc caattcatat tgcctttatt ccgattttaa ttccaccgct attaagcgta
    21781 atgaataaat tgcaaattga ccgccgtgcg gtagcttgtg ctttaacctt tggtttaacc
    21841 gccacttata tgttaattcc ggcaggtttc ggtcagatct ttattgaaag catcttagtt
    21901 aaaaatatca accaagcagg tgaaccgttc caattagtga ctaccacagc ggaaatgacc
    21961 aaagcgatgt taattcctgt ttccggtatg attttaggct tactgtttgc attctttgtg
    22021 tcataccgta aaccgcgtgt gtatattgaa aatatgcctg aaccaaccgc agatgatatt
    22081 gaagcccgtg ttgcaaacgt taagcccttc catatcggca ttagtattgt ggcgattatc
    22141 gcgacctgtt cggtgcaatt agcaacaaat tcgactatta tcggcggctt agtcggtctg
    22201 gttattttcg ccttaggcgg tgtatttaga ctgaaagaga gtaacgatgt cttccaagct
    22261 ggcttacgtt taatggcgat gattggcttt gtgatgattg ccgcctccgg ttttgccggc
    22321 gtggtcaatt caacaggcgg tgtgccggca ttggtagaaa gcctgcgtga tgtgatttca
    22381 tcaaaagaaa tggcagcatt gctaatgtta gtcgttggct tatttattac gatgggtatc
    22441 ggctcttcat tctcgactgt gccgatcatt acctcaattt atgtgccgct ttgtatctca
    22501 tttggcttct cgccactggc gacaatggcg attgtcggtg ttgctgctgc actcggtgat
    22561 gcaggttctc cggcatcaga ttccacactt ggtccaacct ccggcttgaa tgctgacggt
    22621 aaacacgacc acatttggga ttctgttgta ccaactttta tccactttaa cattccatta
    22681 ttagtgttcg gctggattgc agcaatgaca ctttaagcta taatatgcaa aatttttgct
    22741 aaaaatgacc gcttgtagcc attacaagcg gtctattttc ttctttattt tgcaaaccac
    22801 attaggatca ccaatgaaat ttatctcttt taatatcaac ggattacgag ccagaccaca
    22861 tcagctcgaa gcgattatcg aaaaacacca gcccgatgta ctcggcttac aagaaatcaa
    22921 agttgccgat gaagattttc cttacgattt agtcaatcac ttaggctacc acgttttcca
    22981 ccacgggcag aaaggtcatt acggcgtggc attactgact aaaaaagagc ctatagcggt
    23041 gcgtaaaggc ttcccgacag atgtacttga tgctcaaaaa cgaatgatta tggcggatat
    23101 cgagaccgat ttcggcgtgc taaccgtatt aaacggctat ttcccacagg gggaaaatcg
    23161 cagccacgaa accaaattcc cggcaaaaca aaaattctat gctgatttgc agaactactt
    23221 ggaaagcgaa ttaaagccgg aaaatccgat tattattatg ggcgatatga acatcagccc
    23281 gactgatttg gatatcggca tcggcgagcc aaaccgcaaa cgctggctgc gtgacggtaa
    23341 atgttcattc ttacccgaag agcgtgaatg gctagatcgc ttatacagct acggcttggt
    23401 tgatactttc cgtgcaggca acccagacgt gaacgataag ttctcttggt ttgactaccg
    23461 ttcaaaaggt tttgatgata atcgtggctt gcgtattgat ttaattcttg cttcaaaatc
    23521 acttgctgag cgttgcgtgg aaacaggcat tgatttagac attcgagcaa tggaaaaacc
    23581 gtctgaccac tcccctgttt gggcaacctt taaataaagt tgcaaaaaaa tctgagtttt
    23641 caaccgcttg tcagttttta gccctacaag cggttatttt ttctcacttt tttgcacttt
    23701 ttacgctgta ctatatccct attcaaatct gtgctacaat caaacactat ttttagatta
    23761 tttttggatt atgaacaaac gtttaaccgt tattttactg attattgttg ccgtacttgg
    23821 tggctggtat tacagcttac aagagaaaga gaatacgggg cttgatcagc ttattaaaaa
    23881 ggaaggacag cctgaatatg tggggaataa aatgtccacc acggtctatg atctaaaagg
    23941 aaacccacaa tatttcgctc aagctactga aattaaacgc tatgagtcta ctgagcgtac
    24001 tgagtttttt aaacctttta ttgagctttt tgccaaagag tcgacgcaaa aacagtggaa
    24061 agtcagtgcc gattatgcag aaattaccaa agaaaaaatg ttaaatttaa aaggtaatat
    24121 caaacttgtt gcattagatc ctgcatcacg tttgcaacaa atcactaccg atacgttaac
    24181 tgttgattta aatacacagg atatttttac cgaaagtacg gtcaaatcga taggttccgg
    24241 ttttactaca acgggggttg gattaaaagg taacttaaaa caacaagttg cgactttgca
    24301 aaaagatgta aaatcttaca ttgaaccgac cattattcaa gaaactaaag aataatttgc
    24361 ttggaacaag gaataacaat gaaattaatg tttaaatcta ttttagtgag cgtgttactg
    24421 ggtagcagct tttccgctta tgctctaaaa ggcgataccg atcagccgat taatattgat
    24481 tcaggtagcc aatcgttaga tatgactaac aatatcgtga cctttagcga taatgtcgtt
    24541 atcactcaag ggtcgattaa agtgaccgca gcaaacgtca aaattacccg tcaagaaggt
    24601 aaaaaagaaa ctattgacgc tacaggctca cctgtaacat tccaacaaac cttagataac
    24661 ggtaaaccgg taaacggtaa aggcaacagc gttcattatg atttaaactc tgaattttta
    24721 acgctgattg gcaatgcgga actcaaacaa caaggtagct ttattaaagc caataaaatt
    24781 acctatgatg tgaaaaaaca acaacttaaa gctaccagtg gcggtaaaac ccgagtgaaa
    24841 accgtgctaa ttccaaatga acttcaagat aacaagaaaa aataaggtta aatgatatgt
    24901 ctgtgcttta tgccgaacat ttagcaaaaa gctataaaca acgccaagtg gtaaaagacg
    24961 tcagcctgaa tgtcaaatca ggtgaaatcg tgggcttgct cggtccgaac ggcgcaggta
    25021 aaaccaccac tttctatatg gttgtgggtt tggttcgcca cgaccacggc acaattcgta
    25081 ttgacgacca agatatcagc acactaccaa tgcacgacag agcaaaacaa gggattggct
    25141 atctaccgca agaagcatct attttccgcc gtttaagtgt gtacgataac ttaatgtcgg
    25201 tattacaagt gcgtaaaaat atcaatgata aacagcgtaa agagcgtgcc gaagagctga
    25261 ttacagaatt ccatattgag catattcgca atagtttagg tcaatcactt tcaggtggtg
    25321 aacgccgccg tgtggaaatt gcccgagcat tagcagcgaa cccgaaattt attctgttag
    25381 atgaaccttt tgctggggtt gacccaatct cggtaattga tattaaaaaa attatcgtca
    25441 atttaaaaga gcgtggttta ggggtattga ttaccgacca taacgtacgt gaaactctag
    25501 atgtatgtga aagagcctac attgtcggta gcggcgaaat gattgccagc ggcacaccac
    25561 agcaagtgct tgaaaaccct gatgtaaaac gtgtttattt aggcgatcaa ttcaaactct
    25621 aaaaaatccc ctctttgggg atttcatctt tagatgaaaa attatgacaa aactaaccga
    25681 atttctaagc cctgaaaatg ttcgccaagg tgtactgatt tcaagtaaaa aacgagcgtt
    25741 agaactcgtg ggtaaaatcg tggctgaatc aatcaaccaa accgaacagc aatctgaagc
    25801 actctgccct attgaatgct ttggcaattt atttaaacgt gaaaagttag gcacaaccgg
    25861 cttaaatcaa ggcattgcac ttcctcatgc aaaattgccc gcttgcggtg atattgagct
    25921 gactaaaccg attgcggtct ttttacaact ggaagaagcg attgactacg aagcccaaga
    25981 caacaaagaa gtggatttaa tttatgcaat gatgtttccg gaaaatagct gcgaacagta
    26041 taaaagttgc ctacctgcaa ttgcacagaa acttaccgat aaatcgttat taaaacagct
    26101 ccgagcagct gaatccgcag aagatatttg gcaaattttg gtgtatgctg ataatcacct
    26161 aaaagatgaa gaataatact gaggaaatat ggaattaatc attataagcg gacgctcagg
    26221 ctctggtaaa tcggtagcat taagagcatt agaagatgca ggttattact gcgtggataa
    26281 tatcccttta cctttaattc caaccttaac ctactacctt actcaagaaa atcgttcggc
    26341 agtcatcagc ttagatattc gtaatttacc gaatacgcct aatgcgattg aagaattgct
    26401 tacccagctt gagcctttaa atgctaagct gattttttta gattgcgaac gcaatacctt
    26461 aatccgccgt tatagcgatt cccgccgaat ccacccgctt tccacccaag acgatctctc
    26521 gttagaaggt gcattggatt tggaacaaaa attgcttgag cctttggtgc aaaatgcaaa
    26581 ttatattatt gacaccagtc atctttcttc gcacgaatta gcggaaaatc ttcgccaaat
    26641 actgcaaggc tcaaccgaaa aagagcttaa aattattttc caatcattcg gttttaaata
    26701 cggcttacct gccgattccg attacgtttt tgatgttcgc tttttaccga acccacattg
    26761 gaatccggag cttcgcccaa tgacaggctt agagcaaccg gtgattgatt tcttagaacg
    26821 ccaaaccgaa gtgcataatt ttatttacca aacccgcaat tatttagaaa tgtggctgcc
    26881 gatgttggaa caaaataacc gtagctacct aactattgcg attggttgca ctggtggaaa
    26941 acatcgttcc atatttattg ccgagcaact ggcaaaatat ttccaagcaa aaggcaaaaa
    27001 tgtgcaagtt cggcataaaa gtttggaaaa acaccataaa aaaacatctt aaataagtag
    27061 gggctggtcc tgtgccagcc ctcatttcaa ctattttagg gcgggcacag ggcaccgccc
    27121 ctacatttga ttttatgaaa tccgcagata ttgttattgt tggcggcggt atggttgggc
    27181 tggctcttgc cggactgctc aaagatgtcg attgccaaat taaaattatt gagaaaaaca
    27241 cgcccaaatt tgatgccgat acgatttacc accgtgtaag tgcgattaat gcttcaagcc
    27301 aaaaaatact ggaacagatt ggtgcttttc aacacattcc ggctcaacgc ctttctcctt
    27361 acaccgaaat gaaagtgtgg gaaaaagaca gctttgctaa tatccatttt gataataatg
    27421 ataatgaaat caagcaatta ggactttcac aacttggctt tattttggaa aatgcggtga
    27481 ttcaacacgc attgtggcaa caggtttctc aacaagctaa tgtagaatat attatcgccg
    27541 ccccccaaac tctgggcgtg agcgaaaacg gggcattttt aaccttagat aacggcgaaa
    27601 tgctgtctgc taaattggtt gtcggggcag acggtgcaaa ttcgtgggtg cgtaaccaga
    27661 tgaaaatgcc gcttaccagt aaagattatc aacataccgc cttagtctgt aatgtcaaaa
    27721 caaccgaaaa gcacggcaaa accgctcgtc aaatctttgc tgaagatagc atttttgctt
    27781 ttctgccaat ggcagatgag catctctgct cgattgtttg gtcgctgcca ccaaacaaag
    27841 ccaatgaatt aaaaaatgca agcgaagccg aatttaataa agccttaacg attgccttcg
    27901 ataatcagct tggcttatgt gaagtgcaaa gtttgcgtga aatttacccg cttgcagcac
    27961 gcttctgccg tgactttgcc caacctcgta ttgcgttgat tggtgatgcc gctcatacca
    28021 ttcacccatt ggcgggactt ggtgtcaatt taggctttgc cgatgccaga acgctggcaa
    28081 acgaaatcca aaaacagttg gtaaacaacc aagatattgg tgaataccgc catttacgcc
    28141 aatttgaacg tgagcgtaaa gcccaagcga tgaaattatt agccacaatg gaaggattaa
    28201 aacagctatt tgacggcaat cacccgctta aaaaattatt cagaggaatt ggtttaagcc
    28261 taaccaacca gctgcctttt gtgaaaaagc agcttattca gcaagctatt tacttataaa
    28321 atgtaaattt tttcgaaata acgtaaagat gaacgatttt atgaactaat acggagatta
    28381 cttgtcta
//
LOCUS       DANLZC010000014        28279 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.014, whole genome shotgun sequence.
ACCESSION   DANLZC010000014 DANLZC010000000
VERSION     DANLZC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28279
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.014"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..1434)
                     /gene="der"
                     /locus_tag="PXE44_000479"
     CDS             complement(<1..1434)
                     /gene="der"
                     /locus_tag="PXE44_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL5588022.1"
                     /translation="MTPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGH
                     ANIAGYDFIVIDTGGIDGTEEGVEEKMAEQSLLAIEEADVVLFLVDARAGLLPADVGI
                     AQYLRQREKTTVVVANKTDGIDADSHCAEFYQLGLGEVEQIAAAQGRGVTQLIEQVLA
                     PLGEQLNAEQFNQEQAGENAKNVANEETDEWDTEFDFENEEDAALLDEALAEEAEESI
                     EDKNIKIAIVGRPNVGKSTLTNRILGEERVVVYDMPGTTRDSIYIPMERDGQQYTIID
                     TAGVRKRGKVNLAVEKFSVIKTLQAIQDANVVLLTIEAREGISDQDLSLLGFILNAGR
                     SLVIVVNKWDGLSQDIKDQVKSELDRRLDFIDFARVHFISALHGSGVGNLFDSVKEAY
                     ACATQKTSTSMLTRILRMAADEHQPPLVNGRRVKLKYAHPGGYNPPIIVIHGNQVEKL
                     ADSYKRYLSNYFRKSLKIIGSPIRILFQ"
     gene            complement(1619..2926)
                     /locus_tag="PXE44_000480"
     CDS             complement(1619..2926)
                     /locus_tag="PXE44_000480"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDL5588023.1"
                     /translation="MTILALGINHKTASVGLREKVAFVDDKRKLALEQIQTSGLAESV
                     VILSTCNRTELYFHQPNISPREESEENIQWREQCFRWFAQIHQLDESELRECLYFKQN
                     LEAANHLMKVASGLDSLILGEPQILGQVKQAYQYSEDFYQHQQKAMSTKLSRLFQRTF
                     ATAKRVRSETEIGSQAVSVAYAACGLARQIFDNFGKLRFLLVGAGETIELVARYLIQH
                     GAKNIMLANRTHIRAEMLAEKLNAPMQILSLSALQLGLNQADVVISSTGSPDTLISKE
                     MVEIAQKQRQFDPLLLIDIAVPRDIDEKAGDLEGVYAYSVDDLQNIIQRNMAQREEAA
                     KQAAEIVKEESKAFFEWLKQQQSSDLIKRYRQDAEQTRQELLEKALQAISQGQDSQKV
                     LSELSYKLTNQLLHAPTQALQAMAKSGNSTGLQSFSRALRLEE"
     gene            3180..5378
                     /gene="relA"
                     /locus_tag="PXE44_000481"
     CDS             3180..5378
                     /gene="relA"
                     /locus_tag="PXE44_000481"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDL5588024.1"
                     /translation="MVAIRYSHQLEPNNFELSSWCASLNMSPVTFEQIHNAWSYAQEK
                     LDTDYSLMWYGIEMVEVLHGLNMDDDSLVAAMLFPLVKHNVIDLVQLKEDFSNDIKNL
                     VKGVLEMENIRQLSANNVSDLQIDNIRRMLLAMVDDFRCVVIKLAERIAYLRDSNHHS
                     EEDTVLAAKECSHVYAPLANRLGIGQLKWELEDYCFRILHPVSYTQIAKFELGERRVD
                     RERYIANFVADLTACLSPEIKNVQIYGRPKHIYSIWKKMQKKRLRFNQLFDIRAVRVI
                     VPTLEDCYTALGIIHTKYTHLPEHFDDYIANPKPNGYQSIHTVVLGEGEKTIEVQIRT
                     QQMHDDAELGVAAHWKYKEGATAGRSGYEEKIVWLRKLLEWQNDLADSGDMVAEMRSQ
                     IFDDRVYVFTPRGEVIDLPQKATPLDFAYAIHSEIGHRCIGAKVAGRIVPFTYHLQMG
                     DQVEIITQKEPNPSRDWLNPNTGFVNTGKARSKIIAWFKKLDREKNIPIGKEALEAEM
                     EKFGFTLKQIEEYALPRYNLKQFDDLYAGIGGGDIRLHQLMHFLQSKLIKPTAEQEDE
                     AVLKQVNKNTNQPKPSKGYVIVEGVGNLMHSIARCCQPIPGDEIVGYITQGRGISIHR
                     ADCEQLFELQSLNPERVVEADWGEGYTSGLSLTIRVIANDRNGLLRDVSAIMANEKVN
                     VLGVSSRTDVKRSLATIDMEIQLNNVEILNKLLARIAQLDDVIEAKRLSS"
     gene            5497..6693
                     /gene="ispC"
                     /locus_tag="PXE44_000482"
     CDS             5497..6693
                     /gene="ispC"
                     /locus_tag="PXE44_000482"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDL5588025.1"
                     /translation="MKKLVILGSTGSIGKSTLSVVEHNPNPNQYEVFALVGGKNVELM
                     LEQCRQYQPTFAAMDDEKSAQALKTALATLGSKTEVLAGQAAICELAAHSEVDMVMAA
                     IVGAAGLLPTLSAVKAGKTVLLANKESLVTCGQIFIDEARKSGAKLLPVDSEHNAIFQ
                     SLPPEAQQKVGFCPLAELGVSKIILTGSGGPFRTKPLNEFDAITPAQAVAHPNWSMGK
                     KISVDSATMMNKGLEYIEARWLFNATAEEMDIIIHPQSIIHSMVRYIDGSVIAQMGNP
                     DMCTPIAHTMAYPNRINAGVPPLDFFKLKELTFIEPDFARYPNLKLAIEAFAEGQYAT
                     TAMNAANEIAVEAFLNEQIRFTDIVNVNRTVVENIAPIQVSEIADVLHIDKLARELAK
                     QAVLQL"
     gene            complement(6736..8118)
                     /gene="nhaC"
                     /locus_tag="PXE44_000483"
     CDS             complement(6736..8118)
                     /gene="nhaC"
                     /locus_tag="PXE44_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL5588026.1"
                     /translation="MNNYLLKLKPMTAVILSLLTVFILGFTMIGLGWVPQISILAVIV
                     MLLLFGLINKIPFKNMQEGMASGLMSSIGAVYLFFFIGLLVSALMMSGAIPTLMYYGL
                     SVISPELFYISAFALSSVVGIALGSSLTTTATIGVAFIGMASAFGANPAIVAGAVVSG
                     AFFGDKTSPLSDTTTIAASVVGIDMFAHIRNMLYTTIPAWILTALFLWFLTEPTDAQN
                     LANIENLKTNLQETGLIHGYSLLPFVTLILMAIKRVNSIYIIVTTVTLSLIITYIHSS
                     PTLEQLGNYFFTSYKPVEGVDLGEVASLVSRGGINSMFFTITIVLLALSLGGLLQILG
                     ILPALLNGISGFLTSTGRVTLVVASTAVGINVLIGEQYLSLLLSGNTFRSTYERLGLE
                     VKNLSRTIEDAGTVINPLVPWGVCGVFISSVLNVPVLDYLPYAFFCYICLILTLIFGF
                     TGVTISKKAN"
     gene            complement(8164..9750)
                     /locus_tag="PXE44_000484"
     CDS             complement(8164..9750)
                     /locus_tag="PXE44_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDL5588027.1"
                     /translation="MIQITQTINIDLLIVGSGIAGLSAAVEAKEKGINTLLISKSTIG
                     SGASYFPLKATLGIQVTGDEADKIHFKEDIENIGKGKINPNVVQAYIEDSPQAIELLH
                     RIGFQPWKRNDNRPACFAKYPRPIYLIKDWRESALRAKQILQEQHIAFYENATLLHIV
                     TDNNQVQGAVFSLTTSGQISYIFCKTKHLILAAGGIAGLYKDNLYPADVIGSLHYVAQ
                     QAGATLVNLAFIQFIPAFVEPKYKVLFGEHTLKYVTQITDSKGNNLFPHLNAEQFAKM
                     VLERSHYAPFSLDFAGVEFDLVMMKHLLENPHEKGIYLKYSPKLYEDTEEFYTVYLNW
                     LKNEVGIDLVQDKIAIKPFAHSCNGGIQINQWGESAVKGLFAIGEISHCIEGANRMGG
                     NSVGGGLVFAKRAVSEIVRSLEQNDLQKNSEISPLAMAEKALNQLANSTANLELTASE
                     VLSTIREKMALYANVYRTRSNLKLLLEELNRLEQEFDPIYHAQYQGIEIYNALKTAQL
                     VVNQMLNEQSLGAHYLDEQN"
     gene            complement(9813..10619)
                     /locus_tag="PXE44_000485"
     CDS             complement(9813..10619)
                     /locus_tag="PXE44_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5588028.1"
                     /translation="MSMKFIKEQPIIELSNIVVQFASRDGTLFNPKKFTAVNDVSLAI
                     HAGETVGLVGQSGCGKSTLASVMIGLQKPTSGTVKFNGLEMKYGSAEARKYFGSQVSV
                     IFQDPATALNPRMRVLDILKDPMDIHNILQPQEREKRVYDLLSRVGLPRSAALVEPTR
                     LSGGQKQRVAIARALALNPKLIVADEPTSALDVSVRAQVLNLLADLKKELNLAMVFIS
                     HDLQTVHQVSDRIVVMNGGKIVEMGDASDVFERPTQEYTRTLIEAAPSLL"
     gene            complement(10634..12604)
                     /locus_tag="PXE44_000486"
     CDS             complement(10634..12604)
                     /locus_tag="PXE44_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDL5588029.1"
                     /translation="MFRQGLAARLASGGARFRALSTSSKIALLFIVFIALVAIFAPLV
                     APYDPLQTLRPVQAPSGDYLFGTDRLGRDIFSRMVWGARTSLFIGLGAVGVAILFGSI
                     LGATAATADKFGNEVIMRFMDILMAFPGIALAAVLLATFGNSVPVIIVTIAVVYTPQL
                     ARVVRANVVSQWEEDYVRAERVIGGSRTYILLKHVVRNTAAPVLVFATVMVADAIVFE
                     ASLSFLGAGVQPPFPSWGNILSEGRNLVLSGFWWATTFAGIMILLTVLALNILAEGLT
                     DALVNPKLKRSTQSKEDVAKPLATDVQEAMSETLALKRYLLKLHEKETTRTDRMQLNS
                     TAKPILQVKNLSIRFPNRYGETPLVDNISFTVHEGETMGLVGESGCGKSITAFSIMGL
                     LPKTAKITGEILFTDRSGKQHNLLESADLNALRGHEISMIYQDALSALNPSMRIKDQM
                     AQLISRGGKQSAETLLQWVKLDPEKTLNRYPHELSGGQRQRVLIAMALAREPKLLIAD
                     EPTTALDVTVQAEVIKLLNELREKLGFAMVFVSHDLALVAQIAHHITVMYAGQVVETA
                     PTTPLLANPTHEYTRGLLGSVLSTELRAERLYQIPGSVPSPFDFAKGDRFASRSLRPD
                     ANPEQHLKLVPTGDHPQHFWASHLEDPQSKEA"
     gene            complement(12606..13559)
                     /locus_tag="PXE44_000487"
     CDS             complement(12606..13559)
                     /locus_tag="PXE44_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5588030.1"
                     /translation="MEIILRLLWRRLMALPVMILGVTALVFIVLQFTPGDPATVALGE
                     SASEAAKEIYREQHGLNDPLLVQYFRFLGNLFVLDFGMTTPPEQPIVDMIAKAFPLTL
                     QLTFIGVFLAAIVSFTLGVTAALYRDTWIDQLIRLISVAAVATPSFWLGILLIQYFSL
                     KLDWLPSGGFIPFSEDPKGYVNSMVLPSLALAVPVCASLIRVVRTTMVEEMDKDYVRT
                     AIGNGVPYSTVIRHNVLRNALITPVTVLGLRVGYLLGGAVVIEQIFDLPGMGKLIFNG
                     IVNHDLNLVQGVVLTIAFTFVLVNIIVDILYLLINPKIRSL"
     gene            complement(13771..15354)
                     /locus_tag="PXE44_000488"
     CDS             complement(13771..15354)
                     /locus_tag="PXE44_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5588031.1"
                     /translation="MKKQFEHNESRRGFMKLIAGVGAGMAFSGTLGTFTPKAFAAPAA
                     GSTIEAGIAYPISTGFDPLTSSGASSMAANLHIFEGLVDLHPATRKPYLALAAAEPEK
                     IDDVTYRITLRDGAKFHNGNPVTTEDVVYSFERVLDPAKASLFAQFIPFIDTVKKVDD
                     KVVEFKLKYPFALFKERLTIVKIVPKAVVEAGQAAFDANPVGTGPYKFVSATKDDRIV
                     FEAFADYNGGYPAQVEKMTWFLLSDDAARVTAQESGRVQAIESVPYLDAERLKRKGTV
                     ESVQSFGLLFLMFNCEKAPFNNPKVRQALHYGLDTQKLIDVVFLGNAKAATSYVQDTH
                     PDYVKANSQYDFDKAKAEALLAEAGVTELKFELLATDHSWVKECAPLILESWNSLKGV
                     KVSLKHLQSGALYGTHVDKGAYEVVIAPGDPSVFGNDLDLLLSWWYRGDVWPKRRFRW
                     SETAEYAEVQKLLDEAAKNPAASKEAWAKAINIIAEQVPLYPIIHRKLPTAWNDKALT
                     GFQPLPTTGMSFIGVGRAK"
     gene            15576..16733
                     /gene="hemW"
                     /locus_tag="PXE44_000489"
     CDS             15576..16733
                     /gene="hemW"
                     /locus_tag="PXE44_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDL5588032.1"
                     /translation="MNLILPPLSLYIHIPWCVQKCPYCDFNSHAQKGLIPEQEYIEHL
                     LADLSQDLTAYRVAIGDRKLYSIFIGGGTPSLFSAEGIAYLLIEVEKRIAFEPNIEIT
                     LEANPGTAEAERFLGYAQGGVTRISMGIQSFEPEKLLKLGRIHDANEAKQAIKFAQNS
                     AKSGLQSFNIDLMHGLPNQSVKQALADLETGISLNPPHLSWYQLTIEPNTMFYYRKPT
                     LPDDDELWDIFEQGHQLLTNAGYEQYETSAYAKKGYQCRHNLNYWRFGDYLAIGCGAH
                     GKISYPTGEIYRFSKIKHPKGYMRGEYRYSQDLIELDDRPFEFFMNRFRLLEATPKQE
                     FEWFTGLDREVVRPTIDWALNQNYISETATHWQITQHGKLFLNELLEGFLE"
     gene            16876..17358
                     /locus_tag="PXE44_000490"
     CDS             16876..17358
                     /locus_tag="PXE44_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5588033.1"
                     /translation="MIDKQIRNSTAEFLIFTQQNGAENITVRYEDNEIWLTQKLIAQL
                     FDCSTDNVSLHLKNIFNSRELDPNSVTEDFSVTAADVKNYRTKHYNFDAVISIGYRVN
                     SVRATQFRQWATKVLRNFAQKGYVIDRQRMENGTFLNEDYFEQLLAEIREIWLSERRS
                     "
     gene            17327..17524
                     /locus_tag="PXE44_000491"
     CDS             17327..17524
                     /locus_tag="PXE44_000491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588034.1"
                     /translation="MKFGYLNGVLEGKGKMRFDEAKLHAESEFEKYRIVQDRLFESDF
                     DKVLKEMLRSENQYRPSFSTT"
     gene            17611..18078
                     /gene="bcp"
                     /locus_tag="PXE44_000492"
     CDS             17611..18078
                     /gene="bcp"
                     /locus_tag="PXE44_000492"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDL5588035.1"
                     /translation="MNILQAGDKAPKFTLQNQADEPVSLSQFAGKKVLVYFYPKALTP
                     GCTTQACGLRDSKSELDELNVVVLGISTDLPKKLAQFVEKKALNFTLLSDPDHQVAEA
                     FGVWGEKKFMGKTYDGIHRISFLIDEQGGVEQVFDKFKTGEHHQMIVDYLRGR"
     gene            18078..18626
                     /gene="gmhB"
                     /locus_tag="PXE44_000493"
     CDS             18078..18626
                     /gene="gmhB"
                     /locus_tag="PXE44_000493"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDL5588036.1"
                     /translation="MGKKAIFLDRDGTINIDHGYVHQIDDFQFIDGVGKALKQLQDKG
                     YLLVLVTNQSGIARGYFSEAQFMQLTEWMDWSLDEDYGVVLDGIYYCPHHPEGKGEYR
                     KDCDCRKPKAGMFTQAIQDLNIDPSQSYMVGDKLEDILAAEAAGVKTKVLVRTDKAVT
                     EEGQAKADLVLDSLVDLVKYIK"
     gene            18631..19470
                     /gene="queF"
                     /locus_tag="PXE44_000494"
     CDS             18631..19470
                     /gene="queF"
                     /locus_tag="PXE44_000494"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDL5588037.1"
                     /translation="MNYNDKSLSALKLGQKTEYKSEYDASLLQPVPRKLNRDSLGITQ
                     EQPFNRGADVWTCYELSWLNQNGLPQVAIADVAIDFKSENLIESKSFKLYLNSFNQTK
                     FSSIEEVGKTIQADLARCASGQVSVKIYQLSHYTQQPIVDFAGDCIDEQDIQIDSYAF
                     STHYLENIAEDELVEETLVSHLLKSNCLITSQPDWGSVQIRYKGKKLNREKLLRYLVS
                     FREHNEFHEQCVERIFCDLMQFAEPEKLTVYARYTRRGGLDINPFRSNFEEVPENLRM
                     ARQ"
     gene            19662..20795
                     /locus_tag="PXE44_000495"
     CDS             19662..20795
                     /locus_tag="PXE44_000495"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDL5588038.1"
                     /translation="MLIFMMNEYSPNYYIGVMSGTSLDGVDIALLDFAKNPPKMTACD
                     FFPMPEELRADISALLKTGETNLQKLGEIDHRLGLLYAETINAFLAKNQLKAEDIQAI
                     GCHGQTVWHSPDSEYPFTTQIGDMNLVAEQTGITTIADFRRKDMAVGGQGAPLVPAFH
                     QAIFSDPNRLTVVLNIGGISNISVLNPNSPTVGYDVGAGNTLMDSWIEQHQGKRYDKN
                     GEWARTGKLNTELLATLLDESFFAKAPPKSTGRELFNLTWLAKKLENLTACQPQDVQR
                     TLAEFTAQSIANELLRFESSLPRLLLVCGGGSRNPLLMERISALLPEWEVTTTTEFGL
                     DIDYVEAAAFAWLAYQRIHNLPSNMPSVTGAKKAVSLGVIYPA"
     gene            complement(20792..21850)
                     /locus_tag="PXE44_000496"
     CDS             complement(20792..21850)
                     /locus_tag="PXE44_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5588039.1"
                     /translation="MAEILFRSCLCCNAKRIFYLLTFYKGFETSQIATAFSLLVFTSQ
                     AFSLFAGSWGDSFGRYPIMMLGCLLDALAYILLLTTKNYPLLLLATFFFGLGSTLFST
                     NARAFLLANIQDTGDSYQLKTKAQGKFLKVSSLSAMVAPLLTIPFIFYRKAEWLIWIS
                     CCIEIIMLFVMFKTAPKTHECANGQCERFKFGALKGILNEQFIFVHLLLFIPLGLGSA
                     FYIIFPYIFTNLLDKPELVSVSFFVNNLIAVLLQSDFSRKLNLTVHKLIYIAPILIIL
                     QIMPWFYALQWLSTITAFLYLVIFATITVYANTALSNMLVKLDNGKNQGLMFGSSKLI
                     LAITTLGVMNVLPYIFLI"
     gene            21933..22394
                     /locus_tag="PXE44_000497"
     CDS             21933..22394
                     /locus_tag="PXE44_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL5588040.1"
                     /translation="MRKIKLLHVILSLILGLSAAAIGSYPAIKEIGEERAKKHQDILD
                     LNRELYSAMVNRDFSALQTLLSDDFELYSSTGEVIDKQEWIKLIQKRVLEFDKVDLLS
                     TDFQGNQSSGLLRVNGEFFGIERDNVVVEMNIRTIVRNTERQIKCIVIKEV"
     gene            22407..23300
                     /gene="murQ"
                     /locus_tag="PXE44_000498"
     CDS             22407..23300
                     /gene="murQ"
                     /locus_tag="PXE44_000498"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL5588041.1"
                     /translation="MNALGQMITEQRNPNSMNIDRLSALDIVQVINQEDKQVAIAVEQ
                     CLPQIAQAVEKIVQAFEKGGRLVYVGAGTSGRLGVLDASECPPTYGVKPEMVVGLIAG
                     GERALRFPIEGAEDNREQGKVDLQAVNFSEKDVLVGIAASGRTPYVLGAMEYAKQLGA
                     TTVSIASNPNSAMAQIADIAIETVVGPEVLTGSSRMKSGTAQKLVLNMLTTASMILIG
                     KCYQNLMVDVQATNEKLKARAIRIVMQATECEREVAEDTLKLAENNAKLAIMMILGNL
                     DKQKAEDLLSQNQGRLQKALK"
     gene            complement(23384..24328)
                     /gene="prmB"
                     /locus_tag="PXE44_000499"
     CDS             complement(23384..24328)
                     /gene="prmB"
                     /locus_tag="PXE44_000499"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL5588042.1"
                     /translation="MFKYNTELLDEISALPIVNDLVTIQDMMRWGYSYFNASDLYYGH
                     GYDNAWDEAQQLVLAGLYLPFDVPETLYSSRLTRVEKERIINLICERLGSRKPVAYLT
                     NSAWFCGGEYYVDERVIVPRSPIGELIKKGFKGIIKNEPHRILDMCTGSGCIAIACAE
                     RFPEAEVDAVDLSLDALDVAQINIERHQVSHRVFPICSDLFNNLPQDKYDLIVTNPPY
                     VDEEDLGDMPEEFHHEPALALGSGKDGLDLTKRILAQAADYLNDDGVLVCEVGNSMVH
                     LIEQFPSVPFNWLEFEHGGLGVFSLTKEELVKHRHLFA"
     gene            24421..24966
                     /gene="smrB"
                     /locus_tag="PXE44_000500"
     CDS             24421..24966
                     /gene="smrB"
                     /locus_tag="PXE44_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDL5588043.1"
                     /translation="MNDTLLDDEFALFREAAKGTKKLTQNTFVPKAEPRKKLQELREL
                     KEKADTEFYFSDEYEPLLKEENEKVKYLREDVDPYILKQLRRGDFQPELFLDLHGLTR
                     EKAKKELAALILACEREKVYCASVMTGFGTRTLKDQIPRWLVQHPKVMALHQAPKEWG
                     GDASILILVEQPENPEKMFGR"
     gene            25030..25515
                     /locus_tag="PXE44_000501"
     CDS             25030..25515
                     /locus_tag="PXE44_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL5588044.1"
                     /translation="MSEQTFHQLTALLDSHRARYRVVGHPSAGKSEEVAKIRGTEIGQ
                     GAKALVCKVKGNGVKQAVLAILPADYQADLTKIAEHLGGLRASLASPAEVAELTDCVF
                     GAIPPFSFHPDLLLVAEPSLFERYAELAFNAGTLERSIILNTEDYQKIVNPVLVQFAK
                     S"
     gene            complement(25517..26266)
                     /locus_tag="PXE44_000502"
     CDS             complement(25517..26266)
                     /locus_tag="PXE44_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL5588045.1"
                     /translation="MEKRFEINEQGLSIRCKLFYEKELQQIENVVIVLHGFGSSKDLK
                     SNTKFGERLSSKYKGYAALAFDFPCHGDDARKKLSVEESITYLQLVIDYAKTSLNAKN
                     LYAYGTSFGGYLTLKYLAEKGNPFRKIALRAPAVQIYHSLMNRLSEEERQKLAKGREI
                     LWGFDRQMKISQAFFDELTDITQNDYLDYAEQILILHGTEDEMVALSTSQQFADNNVI
                     ELLPIEGADHAFSNPKLMDIAIGKIVEFFAP"
     gene            26438..27301
                     /locus_tag="PXE44_000503"
     CDS             26438..27301
                     /locus_tag="PXE44_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HDL5588046.1"
                     /translation="MLQLILAILCSVTVGVLIKFARQKGIRIDQSIAVNYIVTTTMTF
                     LLLKPDYQGQSITDIIANNPTSYIFVILSITLPTIFLVQAKALEFAGIIRTDAAQRLS
                     LFLPILAAFTIFGEQVTQNKLIALLLAFVALGCLLWKGNQGMEKGGKIAIISLALVWV
                     GYGVNDILFKQIAKSGSAFPVTLSIAFIGAGCFMFIYLLLKRTQWHVPSVLTGLLLGV
                     LNFGNILFYIQAHQAMKDDPTLVFTGMNLGVICLGTFVGAFFFKEKIHKVNYLGVAIA
                     IVAIICLFYWR"
     gene            27425..28018
                     /locus_tag="PXE44_000504"
     CDS             27425..28018
                     /locus_tag="PXE44_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588047.1"
                     /translation="MKLKALSTLIASSMLYSQSFAVPPEKTAEEPQPFFNKTESGYDL
                     VFNDQNYRLIETELNGEKIQFRAFERIVYVNNPIEPDYQTINLYVPEAYYNNGEINGY
                     QADTAPIFLPNSVGGYMPAKAASYDKKRRDGSPGTILVALSKGYVVASVGARGRTLQK
                     EGKYTGKAPSAIIDSKSAAHKACRFESDHWHHSLCKI"
BASE COUNT         8510 a         5578 c         6117 g         8074 t
ORIGIN      
        1 ttggaataaa atccgaattg gcgagccgat aatttttaaa cttttacgga aataattact
       61 taaataacgt ttgtaagaat ccgctaattt ttcaacctga ttgccgtgaa tcacaataat
      121 tggtgggttg tagccacccg ggtgagcata ttttaatttc actcggcgac cattaaccaa
      181 tggtggttgg tgttcatcag cagccatacg caaaatgcga gtcagcattg aggtcgaggt
      241 tttttgcgtt gcacaagcgt aggcttcttt tactgaatca aacaaattcc ccacgcctga
      301 gccgtgcaat gccgagataa aatgcactct ggcaaaatca ataaaatcta aacggcgatc
      361 taactctgat ttcacttgat ctttaatatc ttgcgacaag ccgtcccatt tgtttaccac
      421 aatcaccaat gaacgaccag catttagaat aaagccgagt agtgataaat cctgatcgga
      481 aataccttct cgggcctcaa tagttaatag caccacgttt gcatcttgaa tggcttgcaa
      541 ggttttaatt accgagaatt tctcaaccgc taaattcact ttgccacgtt tacgcacacc
      601 ggcggtatca atgatggtat attgctgacc gtcacgctcc attggaatat aaatagaatc
      661 acgggttgtt cctggcatat cataaactac cacacgctct tcgcctaaaa tgcggttggt
      721 cagtgttgat ttaccaacat tcggacgacc gacaatcgca attttgatat ttttatcttc
      781 aatcgactct tctgcttctt ctgccaaggc ttcatctaat aatgccgcat cttcttcgtt
      841 ctcaaagtcg aattcggtat cccattcgtc tgtttcttca tttgcaacat ttttagcatt
      901 ttcgcccgct tgttcttggt taaattgctc ggcatttagc tgttcgccta aaggagctaa
      961 cacttgctcg ataagctgcg taaccccacg cccttgtgct gcagcaattt gttctacttc
     1021 gcctaagcct aattgataga attcggcaca gtgagagtcg gcatcaatac catcggtttt
     1081 attggcaacc accaccgtgg ttttttcacg ttggcgtaaa tattgagcaa tgccgacatc
     1141 ggcaggtaat aatccggctc gggcatcaac caagaaaagc accacatcgg cttcttcaat
     1201 cgccaataaa gattgttccg ccattttttc ttccacgcct tcctctgtac catcaatacc
     1261 accggtgtcg attacaatga aatcgtagcc ggcaatatta gcgtgaccat atttacggtc
     1321 tcgggttaag ccggggaaat ctgctactaa ggcatctcga gtacgggtta aacggttaaa
     1381 taaagtcgat ttgccgacat taggtctgcc cactagggca acaacaggag tcattttgta
     1441 tcctctatat ccggttattt gcaaaatagt tgcaaaaatt ggcttattat agcggaaatt
     1501 tggggaagtt gaaattagaa gaagatcggg aacacgggga gggacgcacg gcgtgcgtcc
     1561 gcaagcggtt aaaaatgttc aattttttgc aaatatgcct aaaagaatgt gctgagcatc
     1621 attcttctag ccgtaatgct cttgaaaaac tttgtaagcc ggtggagttg ccactttttg
     1681 ccattgcttg taacgcttgg gttggggcgt gtagtaactg gttggtgagt ttgtagctta
     1741 gctcacttaa tactttttgg ctgtcttgcc cttgactgat tgcttgtagg gctttctcca
     1801 acaattcttg gcgggtttgc tccgcatctt gacgatagcg tttgattaaa tcactagact
     1861 gctgttgttt taaccattca aaaaatgctt tgctttcctc tttgacgatt tcagcggctt
     1921 gtttggcggc ttcttcacgc tgagccatat tgcgttggat aatattttgt aaatcatcaa
     1981 cactataagc gtaaacgccc tctaagtcgc cggctttttc gtcaatatca cgaggaacgg
     2041 caatatcaat cagcaaaagt gggtcaaatt ggcgttgttt ttgcgcaatt tccaccattt
     2101 ctttactgat tagggtatca gggctaccgg tggagctaat aactacatca gcttggttta
     2161 aaccgagttg caaagcactt aacgacagaa tttgcattgg agcgtttagt ttttccgcca
     2221 acatttccgc ccgaatatgg gtgcggtttg ccaacattat atttttcgcc ccgtgttgaa
     2281 ttaaatagcg ggcgactaat tcaatggttt cgcctgcccc taccagcaaa aagcgtaact
     2341 tgccaaaatt atcgaaaatt tgacgggcta aaccgcaagc tgcataagca actgacaccg
     2401 cttggctgcc gatttcggtt tcggaacgta ctcgttttgc ggtggcaaag gtgcgttgga
     2461 acaagcgtga tagcttggtg gacatcgctt tttgctgatg ttggtagaaa tcttcgctat
     2521 attgataggc ttgtttaacc tgccctaaaa tttgtggttc gcctaaaatt agtgaatcta
     2581 atccgcttgc cactttcatt aagtggttag cggcttctaa attttgttta aaatataaac
     2641 attcacgcaa ctcgctttca tcaagttgat gaatttgggc aaaccagcgg aaacattgtt
     2701 cacgccattg gatattttcc tcgctctctt cacggggaga aatattaggc tggtggaagt
     2761 agagctcggt gcggttgcag gtagagagaa tgaccacgct ttcagccaat ccgctcgttt
     2821 ggatttgttc aagagccagt ttacgtttgt catctacaaa cgccactttc tctcttaaac
     2881 ccacagaggc ggttttatgg ttgattccta aagctaaaat tgtcattgct acccacaaaa
     2941 ataaagatca aaataaaaac gcaagtattt taatcaagag cgtgaagtcg tgcaaatgat
     3001 tgtgttgata ggtttgattt atcacaaggt tgctacctct taaggagtaa agaggataag
     3061 ataacttgga ttaaattttc gattaggggt aaattttttc tgaatttcca cgtataataa
     3121 aactccattt taagctgtac aagcggtcta atttccacaa aaatttacaa ggaggcctta
     3181 tggtagcaat tcgttattcc catcaacttg aaccgaataa ttttgagttg tcgagttggt
     3241 gtgcaagcct gaatatgtct cctgtgactt ttgagcagat tcacaatgcg tggagttatg
     3301 cacaggaaaa actggatacc gactatagtc tgatgtggta tggcattgag atggttgaag
     3361 tattgcacgg cttgaatatg gacgatgaca gcttagttgc cgcaatgctt tttcctctgg
     3421 taaaacacaa tgtgatcgac ttggtgcaat taaaagaaga tttcagtaac gacatcaaaa
     3481 acttagttaa aggcgtactc gaaatggaga atatccgcca gcttagtgcc aataatgtgt
     3541 cggatctgca aatcgacaat atccgtcgca tgttgttggc gatggtggac gatttccgct
     3601 gtgtggtgat taaacttgcc gaacgaattg cctacctgcg tgatagtaac caccattccg
     3661 aagaagatac cgttctcgcc gctaaagaat gttctcacgt ttatgcaccg cttgccaatc
     3721 gattgggtat cgggcagctc aaatgggagc tggaagatta ctgtttccgc attttacacc
     3781 ctgtctctta cactcaaatt gctaaatttg aattgggcga acgccgtgtg gatcgtgagc
     3841 gttatattgc aaattttgta gcagatctga ccgcttgttt aagtcctgaa atcaagaatg
     3901 tgcagattta tggacgcccg aaacatatct acagcatttg gaagaaaatg cagaaaaaac
     3961 ggttgagatt taatcagctt ttcgatattc gggctgtgcg ggtaatagtg ccaacgttag
     4021 aagattgcta cacggcatta ggcattattc acaccaaata tacccatttg ccggaacatt
     4081 tcgatgatta tattgctaac ccaaaaccta acggttatca gtctattcat acagttgtat
     4141 taggcgaagg tgaaaaaacg attgaagtgc aaattcgtac ccagcaaatg cacgatgatg
     4201 cggagctagg cgtagccgct cactggaaat ataaagaggg ggcaaccgca gggcgttcgg
     4261 gctacgaaga aaaaatcgtg tggctacgca aattgcttga gtggcaaaac gatcttgccg
     4321 attcaggcga tatggtggca gaaatgcgtt cgcaaatttt tgatgatcgt gtttatgtct
     4381 ttaccccaag aggcgaggtg atcgatttac cgcaaaaagc tacaccactt gattttgctt
     4441 atgcaattca tagtgaaatc gggcatcgtt gcattggagc aaaagtggca gggcggattg
     4501 tgccgtttac ttatcatctg caaatgggcg atcaggttga aattatcacc caaaaagagc
     4561 cgaacccaag ccgagattgg ctcaatccga ataccggttt tgtgaataca ggcaaagctc
     4621 gctcaaaaat tattgcgtgg tttaaaaaat tagaccgaga gaaaaatatt ccaatcggca
     4681 aagaggccct tgaagccgaa atggaaaaat tcggttttac gctcaagcaa attgaagaat
     4741 atgctctgcc tcgctataac ttaaaacagt ttgatgatct ctatgccggt attggcggtg
     4801 gcgatattcg tcttcatcag ttaatgcact tcttgcaatc aaaattaatt aaaccgaccg
     4861 ccgaacaaga agacgaagcg gtacttaaac aggtgaataa aaacaccaat cagccgaagc
     4921 caagcaaagg ctatgtgatt gtagagggcg tagggaactt aatgcattcc attgcccgtt
     4981 gctgccagcc gattccgggt gatgaaatcg tgggttacat cacacaaggc agagggattt
     5041 cgattcaccg tgcagattgt gagcaactgt ttgaattaca aagtctcaac ccagaacgag
     5101 ttgtggaggc ggattggggc gaaggctata cctctggttt aagcttaacc attcgagtga
     5161 ttgccaatga ccgcaacggc ttactgcgag atgtcagtgc aattatggca aatgaaaaag
     5221 taaacgtgtt aggcgtttcc agccgaaccg atgtgaaacg tagccttgcc accattgata
     5281 tggaaattca gcttaacaac gttgaaattt tgaataaatt attggctcgt atcgctcaat
     5341 tagatgatgt tatcgaagca aaacggcttt cgagttaatc tacaagcggt tgtttttgct
     5401 aaacaattta caaacaacga atgagtatcc gcataattac ggcgtagggg cgaaaaatct
     5461 ttcgccctta tcagtcatta gataaagaga aacaaaatga aaaaattagt gattttaggt
     5521 tcaacaggtt ctatcggtaa aagcacttta tcggtagttg agcataaccc aaacccaaac
     5581 caatatgagg tgtttgcgtt agttggtgga aagaatgttg aattaatgct ggagcaatgt
     5641 cgtcaatatc agccgacttt tgcggcaatg gatgatgaaa aatccgcaca agcattaaaa
     5701 acggcattgg caactttggg gtctaaaact gaagtgttag ccggtcaagc agctatttgt
     5761 gagcttgctg cacattccga agtggatatg gtaatggctg ctattgtagg agcagcaggg
     5821 ttattaccga cactttctgc ggtgaaagcg ggtaaaaccg tattgttggc aaataaagaa
     5881 tcgctggtaa cctgtgggca aatttttatt gatgaggctc gtaaatccgg tgcgaagctc
     5941 ttaccggtag atagtgaaca caatgcgatt ttccaatcgc ttccaccgga ggcacagcaa
     6001 aaagtcgggt tttgtccact cgctgaactg ggtgtcagta aaattatttt aacaggatcg
     6061 ggtggaccgt tccgtactaa gccattaaat gaatttgatg cgattacacc ggctcaagca
     6121 gttgctcacc ccaattggtc tatgggtaag aaaatttcag tcgattcagc cacaatgatg
     6181 aacaaaggct tggagtatat tgaagcccgc tggttgttta atgcaacagc cgaggaaatg
     6241 gatatcatca ttcacccaca atcaattatt cattcgatgg tgcgttatat tgatggctcg
     6301 gtgattgctc aaatgggcaa cccggatatg tgtacgccga ttgctcacac gatggcatat
     6361 ccaaaccgaa ttaatgctgg tgtgccacca ttagatttct ttaaattaaa agagctaacc
     6421 tttatcgagc cggattttgc ccgttacccg aacttaaaat tagcgattga ggcttttgca
     6481 gaaggacagt atgcgacaac agcgatgaat gccgccaatg aaattgccgt tgaggcgttt
     6541 ttaaacgagc aaatccgctt tacggatatt gtgaacgtga atagaacggt ggtggaaaat
     6601 attgctccaa ttcaagtcag tgaaattgct gatgtattgc atattgataa actcgccaga
     6661 gagttagcca aacaagctgt tcttcagctc taatgaaaag aaaatgccta caaatttgta
     6721 ggcatttttc acatattagt tagctttttt actgattgtt accccggtaa atccaaaaat
     6781 taaggtaagg attaaacaga tataacagaa gaatgcgtat ggtagataat ctaacaccgg
     6841 tacatttaat acgctactga taaacacacc gcacacgccc caagggacta atgggttgat
     6901 taccgtgcct gcatcttcaa tggtgcgaga taagtttttc acttctaagc ctaaacgttc
     6961 ataagttgag cggaaagtat tacctgataa caataagctc aaatattgct caccgattaa
     7021 gacgttaatt cccaccgcag tgctggcaac tacaagagta acacgtcctg tgctggttaa
     7081 aaagccgcta atgccgttaa gtagtgccgg taaaatgccg aggatttgta aaagaccacc
     7141 taaacttaac gcaagcaata caatagtgat ggtaaagaac atactattaa tgccaccacg
     7201 agagactaat gatgccactt cgcctaaatc tacgccttca acaggtttat agctggtaaa
     7261 gaaataattc cctagctgtt caagggttgg cgaactgtgg atataggtaa taattagcga
     7321 gagtgttact gtagttacaa taatataaat tgaattaaca cgcttaattg ccattaaaat
     7381 taacgtaaca aaaggtagca aagagtagcc gtgtattaat cctgtttctt gcaaattggt
     7441 ttttaggttc tctatatttg ctaaattttg tgcatcagta ggttcggtta aaaaccaaag
     7501 gaataatgct gttaaaatcc acgcagggat ggtggtataa agcatattac ggatatgagc
     7561 gaacatatca atgcccacta cggaagcagc aatggtagta gtgtctgaaa gtggagaggt
     7621 tttatcgcca aaaaacgccc ctgaaaccac cgcacctgct acaatcgcag ggttcgcacc
     7681 gaaagcagaa gccataccga taaaagcaac accgatggtg gcggttgtgg ttaaactact
     7741 gcctaacgca atacctacta cagaacttaa tgcgaatgca gaaatgtaaa ataattcagg
     7801 tgaaatcaca cttaagccat aatacattaa ggttggaatc gcaccagaca tcatcaaagc
     7861 tgaaaccaat aatccaataa aaaagaatag gtaaaccgca ccaatactgg acatcagccc
     7921 gcttgccata ccctcttgca tatttttaaa cggaatttta ttgattaagc cgaaaagtag
     7981 cagcattaca atcacggcta gaattgaaat ttgaggcacc cagccaagcc caatcattgt
     8041 aaaacctaag ataaagaccg tcaaaagcga aagtatgacc gctgtcattg gttttaattt
     8101 aagtaggtag ttattcattt atactcctaa tttattattt ttaaataagt tggctattat
     8161 agactaattt tgttcatcta agtagtgcgc gcctaaactc tgctcattta gcatttgatt
     8221 gaccactaat tgagcggttt ttaacgcatt gtaaatttca atgccttgat attgagcgtg
     8281 atagattggg tcaaattctt gttccaaacg gtttaattcc tcaagtagta gtttgaggtt
     8341 gctccgagtg cggtacacat tggcgtataa cgccattttc tctcgaatag tagaaagcac
     8401 ttcactggca gttagttcta gattagcagt tgaattagca agctgattta atgctttttc
     8461 tgccattgca agcggtgaga tttcgctgtt tttttgcaaa tcattctgtt ctagcgaacg
     8521 aacaatttcc gataccgcac gtttggcaaa cactaagcca ccgccgactg agttaccacc
     8581 catacggttt gccccttcaa tgcagtgaga aatctcacca atcgcaaata gcccttttac
     8641 tgcactttcg ccccattgat taatttgaat gccgccgtta cagctatgag caaacggctt
     8701 gattgcaatc ttatcttgca ctaaatcaat cccgacttca tttttgagcc aattcaaata
     8761 gacggtataa aactcttccg tgtcttcata cagtttgggg ctgtatttaa ggtaaatgcc
     8821 tttttcgtga gggttttcaa gcaaatgttt catcatcact aaatcgaact ccacgccggc
     8881 aaaatcaagg ctgaaaggag cataatggct acgttccagc accatttttg caaattgctc
     8941 ggcatttaag tgtggaaata gattgttacc cttagaatcg gtgatttggg tgacatattt
     9001 caatgtatgt tcgccaaata gcaccttgta ttttggctcg acaaaggcag gaatgaactg
     9061 aataaaggca agattaacca aggttgcccc ggcttgttgt gccacataat gaagtgaacc
     9121 gataacatca gccgggtaga gattatcttt atacagccca gcaatcccgc ctgcggctaa
     9181 aataagatgc ttggttttgc aaaaaatata ggaaatttga ccgcttgtag tgagcgaaaa
     9241 taccgcacct tgcacttggt tattgtctgt gacgatgtgt aaaagggtag cattttcata
     9301 gaaagcaatg tgttgctctt gcaaaatctg tttcgctcgt agggcagatt cccgccaatc
     9361 tttaattaaa taaattgggc gagggtattt ggcaaaacaa gccggacggt tgtcgttacg
     9421 cttccaaggc tgaaagccga tacggtggag tagttcaatc gcttgtggtg aatcttcgat
     9481 ataggcttgt accacattag ggttgatttt gcctttgccg atgttttcaa tatcttcttt
     9541 aaagtgaatt ttatcggctt catcgccggt tacttgaatg ccaagtgttg ccttaagcgg
     9601 aaagtagctc gctcccgagc caatcgtaga tttgctaatc agtaaggtat taatgccttt
     9661 ttcttttgcc tcaactgcag cagaaagccc agcgatgcca gagccgacaa ttaataaatc
     9721 aatgttaatg gtttgggtaa tttggatcat aaataaaaac acaagcggta aaatttgcga
     9781 aaaattttac cgcttgtagg cacaaagcac aattataata aagatggtgc tgcttcaatt
     9841 aaggttcggg tgtactcttg cgttgggcgt tcaaatacgt ctgaagcatc gcccatttcc
     9901 acaattttac cgccgttcat aaccacaatg cggtctgaaa cttggtgaac ggtttgtaag
     9961 tcgtgagaga taaataccat cgctaaattg agttcttttt tcaagtctgc cagcaggttc
    10021 agtacttgag ctcgaaccga tacgtcaagt gcagaggttg gttcatcagc cacaatcagc
    10081 ttcggattta atgccaatgc acgagcaatc gctacacgtt gcttttgccc accagataat
    10141 cgggttggct cgaccaacgc cgctgaacga ggtagaccga ctcgagagag caaatcataa
    10201 acccgcttct cacgttcttg cggttgtaag atattatgaa tatccatcgg atcttttaaa
    10261 atatccaaca cacgcatacg cggatttagg gcggttgccg ggtcttggaa aattactgac
    10321 acttggctac cgaaatattt gcgagcttcc gcactgccat atttcatctc taagccgtta
    10381 aattttactg tgccggaggt tggtttttga agcccgatca tcacactggc taaagtagat
    10441 ttgccacaac cagattgtcc aaccagaccg actgtttcac cggcgtgaat tgctaggctg
    10501 acatcattta ccgccgtgaa ttttttcggg ttaaataagg tgccgtcacg agaagcaaat
    10561 tgcacaacaa tattagacag ttcaataatt ggctgttctt tgataaattt catactcatc
    10621 gtattgctct cctttatgct tctttagatt gtggatcttc taagtgtgaa gcccagaagt
    10681 gttgtgggtg atcaccggtt ggcactaatt tcaagtgctg ttcagggttt gcatctggtc
    10741 ttaatgaacg actcgcaaaa cggtcacctt tcgcaaaatc aaatggtgaa ggcacactgc
    10801 ccggaatttg gtataggcgt tctgcacgca attcggtaga aagtaccgag cctaataatc
    10861 cacgtgtgta ttcgtgtgtt gggtttgcca ataatggcgt agttggtgca gtttcaacca
    10921 cttgaccagc atacattacc gtgatgtggt gagcgatttg tgccactaaa gctaagtcgt
    10981 ggctcacgaa taccattgca aatccaagtt tctcacgtaa ttcgtttaat aatttgatca
    11041 cttcagcttg aacggttacg tctaatgctg ttgttggttc atcagcaatc aatagtttcg
    11101 gttcacgagc aagtgccatt gcaattaata cacgctgacg ttgtcctcca gataactcgt
    11161 gaggataacg gttgagcgtt ttttccgggt caagttttac ccattgcaat aatgtttctg
    11221 ctgattgttt accaccacgg ctgattaatt gtgccatttg gtctttaata cgcattgatg
    11281 ggtttaacgc acttaatgca tcttggtaaa tcatcgagat ttcgtgacca cgaagggcat
    11341 ttaaatcggc tgattctaaa agattgtgtt gtttacctga acggtcggta aagaggattt
    11401 caccagtgat ttttgcagtt tttggtaata aacccataat tgagaatgcg gtgattgatt
    11461 taccacaacc agattcaccc actaagccca tggtttcgcc ttcgtgaaca gtaaagctga
    11521 tattatcaac aagcggagtt tcaccataac gatttgggaa acggattgat aagtttttca
    11581 cttgtaaaat cggtttggcc gttgagttta attgcatacg gtcagtacgg gtcgtttctt
    11641 tctcgtgtaa tttgagtaag taacgtttta acgcaagagt ttcagacatt gcttcttgaa
    11701 cgtctgttgc caatggtttt gcgacatctt ctttactttg agttgaacgt tttagttttg
    11761 gatttactaa cgcatcggtt aagccttcag caaggatatt taacgcaagt acggttaata
    11821 aaatcataat ccctgcgaaa gttgtagccc accagaagcc acttaataca aggttacgac
    11881 cttcagataa gatatttccc caagatggga atggaggctg gacacctgca cctaagaatg
    11941 aaagtgatgc ttcaaatacg atggcatcag caaccatcac tgttgcgaat actaaaaccg
    12001 gtgcagcagt attacgcaca acgtgtttta atagaatata agtacggcta ccaccaatta
    12061 cacgctcggc acgcacataa tcttcttccc attgagatac tacgttagca cgcaccactc
    12121 gggcaagttg gggagtgtaa acaaccgcaa tagtcacgat aatcacaggt acagagttac
    12181 cgaaagtggc aagtaataca gcagctaatg cgatacccgg gaatgccatt aagatatcca
    12241 taaaacgcat aatcacttcg ttaccgaatt tatcggcggt tgccgcagtt gcccctaaaa
    12301 tactaccgaa tagaatcgca acgcctaccg cacctaaacc gataaagaga gatgttcttg
    12361 ctccccatac catacgcgag aaaatatcac gtcctaaacg gtctgtaccg aataaataat
    12421 caccgcttgg agcttgcact ggacgtagcg tttgcagtgg gtcataaggc gctactaatg
    12481 gtgcaaaaat agcgactaat gcgataaata caataaagag taaggcgatt tttgagctgg
    12541 ttgataatgc tctaaatctt gcaccgcctg aagcaagtcg agcagctaat ccttggcgaa
    12601 acatattata aactccgaat ttttggatta atgagcaagt aaagaatatc aacaatgata
    12661 ttcaccaaca cgaaagtaaa tgcgatagtt aatactacac cttgcactaa gttcaagtcg
    12721 tgattgacga taccgttgaa aattaattta cccattcccg gtaagtcgaa gatctgttca
    12781 atcacaactg caccacctaa taaataacca acccgtaaac ctaatactgt taccggagta
    12841 attaaggcat tacgaagcac gttgtggcga atcaccgttg agtaaggtac accattaccg
    12901 atggctgttc gaacataatc tttatccatt tcttctacca ttgttgtacg cacgacacgg
    12961 atcaatgagg cacatacagg aacagctaaa gcgagtgaag ggagaaccat tgagtttaca
    13021 tagccttttg gatcttcgct aaatggaata aaaccgcctg aaggcaacca atctaatttt
    13081 aaggagaagt attgaatcaa taaaataccc aaccagaaag aaggtgtggc aacagcggca
    13141 accgagatta aacggattaa ttgatcaatc caagtatcac ggtaaagtgc tgccgttaca
    13201 cctaatgtga aagagacgat agcagctaag aagaccccaa taaaggtaag ctgtaatgtt
    13261 aaagggaatg ctttagcaat catatcaaca ataggctgtt ctggaggggt tgtcataccg
    13321 aaatctaata cgaataaatt ccctaaaaag cgaaaatatt gtacaagcaa tggatcattt
    13381 aacccatgtt gttcacgata gatttcttta gcggcttcgc tggcgctttc accgagagca
    13441 acggttgccg gatctccggg ggtgaattgt aaaacaataa ataccaaggc agttacgcct
    13501 aagatcatta ccggcagagc cattaggcga cgccataata atcggagtat aatttccatt
    13561 ttcttctcct tgagtgtagt tacactccaa aattaaatct aagtttacct acaagctaat
    13621 atagtgatat gtaggattat aaatgaaaat atagaaatgg taagaattct gagtgatata
    13681 agacgaatat tacatcattt tgatttttat ttatgcaagc ggtaaatttt ttgtaaaata
    13741 ttaccgcttg catttagcta gccgttacga ctatttagca cgaccaacac cgataaatga
    13801 catacctgtt gttggtaatg gttggaaacc tgttaatgcc ttatcattcc acgcagttgg
    13861 caatttacgg tgaataattg ggtaaagagg cacttgttca gcgataatat tgattgcttt
    13921 cgcccaggct tctttagaag cagcaggatt tttggccgct tcatcaagca gtttttgtac
    13981 ttctgcatat tcagctgttt cagaccagcg gaaacgacgt ttcggccata catcaccacg
    14041 ataccaccag cttaataata ggtctaagtc attaccgaaa actgatgggt cgccaggtgc
    14101 aatcacaact tcgtatgcac ctttatcaac gtgtgtgcca tataatgcac ctgattgtaa
    14161 gtgtttgagt gaaactttca cgccttttaa gctgttccac gattcaagaa ttaatggcgc
    14221 acattctttc acccaagagt gatcggttgc caacaattca aattttaact ctgtcacacc
    14281 cgcttctgct aataacgctt cggctttcgc tttatcgaaa tcatattgcg aatttgcttt
    14341 cacataatcc gggtgagtat cttgcacgta agatgtagca gcttttgcat tgcctaagaa
    14401 caccacatca attaattttt gggtatctaa gccataatgt aacgcttggc gaactttcgg
    14461 gttgttaaac ggtgcttttt cacagttaaa cattaagaac agtaagccga atgattgtac
    14521 tgactcaacg gtacctttac gttttaagcg ttctgcatct aagtaaggca cagactcaat
    14581 tgcttgaaca cggccagatt cttgagcggt tacacgagcg gcatcatcag aaagtaagaa
    14641 ccaagtcatt ttctcgactt gtgccgggta gccaccgtta taatctgcaa atgcttcgaa
    14701 aacaatacga tcatctttag ttgcggatac aaatttatat gggccagtcc ccactggatt
    14761 agcatcaaat gcagcttgac cggcttctac cactgctttt ggtacgattt taacaatggt
    14821 taggcgctct ttgaataacg caaatggata tttcaatttg aattcaacca ctttgtcatc
    14881 aacttttttc acagtgtcaa taaatggaat gaattgtgcg aaaagtgatg ctttagctgg
    14941 gtctaataca cgttcaaatg agtaaactac gtcttcagtg gtaaccggat taccattgtg
    15001 gaatttagcc ccgtcacgta aagtaattcg ataggttaca tcatcaattt tttcaggctc
    15061 tgctgctgcc aatgctaaat atggtttgcg agtggctgga tgtaagtcca cgaggccttc
    15121 aaaaatatgg aggtttgccg ccattgagga tgcaccgctt gaggtaagcg ggtcaaatcc
    15181 ggttgatatg ggataagcta taccggcctc aatcgttgag ccggcggcag gggctgcaaa
    15241 tgctttaggt gtaaatgtac ctaaagtgcc actaaatgcc ataccggcac cgacacctgc
    15301 aattagtttc ataaaaccac ggcgggattc gttgtgttca aactgttttt tcatcttaaa
    15361 gctcctttaa gtttttatga taggggcggt attatttgct gaagtgaatt tatcaaaaaa
    15421 tgcacttttt tgaattgaat ttatcatatc attaatgtca aagtggtaaa gcaagaatta
    15481 ttgaatttgt gatctagctc acgaaaatct ttgcttttca cttcacgtgc ttttcgttat
    15541 tattcgccaa tttaatcata aatgaaaaat tgacagtgaa cctgatttta ccgcctttaa
    15601 gcctttatat ccatatccct tggtgcgtgc aaaaatgccc ttattgcgat tttaactcgc
    15661 acgctcaaaa aggattaatt cccgagcaag aatatatcga acatttactt gcagatttat
    15721 cccaagattt gaccgcttac cgagtggcaa taggtgatcg taaattatat tctattttta
    15781 ttggcggcgg caccccgagc ttattttcag cggaagggat agcttattta ttaattgaag
    15841 tagaaaaacg gattgcattc gagccaaata tcgaaattac actggaagcg aacccgggaa
    15901 ccgcagaggc agagcgattt ttaggttacg cacaaggtgg cgtaacccgc atttcaatgg
    15961 gaatccaaag ttttgagccg gaaaaattat tgaaattagg acggattcac gatgcaaatg
    16021 aagcaaaaca agcgattaaa tttgcccaaa attctgcaaa atcgggcttg caaagtttca
    16081 acattgattt aatgcacggc ttaccaaacc aatcggttaa acaagccttg gcggatttag
    16141 aaacagggat ttcactcaat ccaccgcatt tatcgtggta tcagctcacg attgagccga
    16201 atacgatgtt ctattatcgt aagccaacct tgcctgatga tgacgagtta tgggatattt
    16261 tcgagcaagg gcatcaactt ttaacgaatg ccggctatga gcagtatgaa acctcggctt
    16321 atgccaaaaa aggctaccaa tgcagacaca atttaaatta ttggcgattt ggggattatt
    16381 tggcgattgg ttgtggcgct cacggcaaaa tttcatatcc gaccggggaa atttatcgct
    16441 ttagcaaaat caaacacccg aaaggttata tgcgaggtga atatcgctac agccaagatt
    16501 tgattgaatt agacgaccgc ccgtttgagt tttttatgaa ccgctttaga ttacttgaag
    16561 cgacaccaaa acaagaattt gaatggttta cagggttaga cagggaagtt gttcgcccga
    16621 caatcgattg ggcgttaaac caaaactata tttccgaaac cgcaacccat tggcaaatta
    16681 cccaacacgg taaattgttt ttaaatgaat tactggaagg atttttggaa taatttgcaa
    16741 aaaaacgact aaaaaaagac cgcattgtaa tgctaaatta caggcgtttt ttgtgatctg
    16801 cgtcgaaaaa tcctcatttt ttactagaca aaaaaacagt atttagtagc atagccttca
    16861 caataaggag agcttatgat cgataaacaa atccgtaata gtaccgccga atttttgatt
    16921 tttactcaac aaaatggggc agaaaatatt accgttcgct atgaagataa cgaaatttgg
    16981 cttacgcaaa aattaattgc tcagcttttt gattgctcaa cagataatgt ttctttgcat
    17041 ttaaaaaaca tttttaatag tagggaactc gatccaaatt cagttaccga ggatttctcg
    17101 gtaactgccg cagatgtcaa aaattatcgt accaaacact acaactttga tgccgttatt
    17161 tcgataggct atagggtaaa ctcggtgcgg gcgacacaat ttcgccaatg ggcaactaaa
    17221 gtattacgga atttcgccca aaaaggctat gtgattgacc gccaaagaat ggaaaatggt
    17281 acatttttaa atgaagatta ttttgagcag ttgctggcag aaattcgtga aatttggtta
    17341 tctgaacggc gttcttgagg gcaaaggcaa aatgcgtttt gatgaagcga aattgcacgc
    17401 agaaagcgaa tttgagaaat accgcattgt tcaagacaga ttatttgaaa gcgattttga
    17461 caaggtattg aaagaaatgt tgcgttcgga aaatcaatac cgtccatcat tttcaacaac
    17521 ataaaacaac cgcattttgc aaattttttg ccaagtttga ccgcttgccg tgtataatgc
    17581 aagcggttat tttttatgga aaaattacaa atgaatatct tacaagcagg cgataaagcc
    17641 cctaaattta cccttcagaa tcaggctgac gagccggttt ctctctctca atttgccggc
    17701 aaaaaagtac tggtttattt ttatccgaaa gctcttacac ccggttgcac tacgcaagcg
    17761 tgcggactgc gtgacagcaa atcagaactt gatgagctca atgtggttgt tctcggaatt
    17821 agcaccgatt taccgaaaaa attagctcaa tttgtagaga aaaaagcact taattttacc
    17881 ttattatctg acccggatca ccaagttgca gaagcctttg gtgtatgggg cgaaaagaaa
    17941 tttatgggaa aaacctatga cggcattcat cgcatcagtt ttttaattga tgagcagggg
    18001 ggggttgagc aagtgtttga taaatttaaa accggcgaac atcaccaaat gatcgtggat
    18061 tatctacggg gtcgctaatg ggaaaaaaag ccattttttt agaccgtgac ggcacaatta
    18121 atatcgatca cggctatgtg catcaaattg atgattttca atttattgac ggtgtcggca
    18181 aggcgttaaa acagctacaa gacaaaggtt atttattggt gctggtaacc aatcaatctg
    18241 gcattgcccg aggctatttt agcgaagcac aatttatgca actaaccgaa tggatggact
    18301 ggtcgctcga tgaagattat ggcgtggtac tagacggtat ttactactgc ccacatcacc
    18361 ccgaaggcaa aggcgaatac cgtaaagatt gtgattgccg taagcctaaa gcaggtatgt
    18421 ttactcaagc gattcaagat ttaaacatcg acccaagcca atcttatatg gtgggcgata
    18481 aattagaaga tatccttgcc gcagaagccg ccggcgtgaa aaccaaagtg ctggttcgca
    18541 ctgacaaagc agtgacggaa gagggtcagg ctaaagccga tttggtgtta gatagtttag
    18601 ttgatttagt aaaatatatt aaataaagca atgaattata acgataaatc cctttccgcc
    18661 ctgaaattag ggcaaaaaac cgagtacaaa agcgaatatg atgcctcgct tttgcaacct
    18721 gttcctcgta aattaaaccg tgatagttta ggcattacac aagagcagcc ttttaaccga
    18781 ggggctgatg tgtggacttg ctatgagcta tcttggttga accaaaatgg gctaccgcaa
    18841 gtagcgattg cagatgtcgc cattgatttt aagagcgaaa acttaattga atcgaaaagt
    18901 ttcaagctct atttgaacag ttttaaccaa accaaattta gctcaataga agaagttggg
    18961 aaaacgattc aagcggatct ggctcgctgt gcaagcggtc aggtttcggt aaaaatttac
    19021 caattatcac actacacgca acagccgata gttgattttg caggcgattg cattgatgag
    19081 caagatattc aaatcgacag ttatgcgttt tcgactcatt atttagagaa cattgcagaa
    19141 gatgagttgg tggaagaaac cttagtcagc catttgctca aatctaactg cttaattacg
    19201 tcacagcctg actggggaag tgtgcaaatt cgctacaaag gcaaaaagct aaaccgtgaa
    19261 aaactgttac gttatttagt gtcgttccgt gagcataatg agttccacga acaatgtgtg
    19321 gaacgcattt tctgtgattt aatgcaattt gccgagccgg aaaaattaac agtttacgca
    19381 cgctacacca gacgaggcgg gctagacatc aatccgttta gatcgaattt tgaggaagtg
    19441 ccggaaaatc taagaatggc acgccaatga ttttgttgtt ttttgttccc gtctgaaccg
    19501 cctgagcgtg tttgaattgg taggtaaatt tactctgagc ggagcgaatg taaatttgcc
    19561 gttaagccaa ttcaaactaa gcgaggatag cggtgaagct cgggttgcct ttcttttggt
    19621 tacttttctt tggcaaagca aagaaaagta acaaatagca gatgttgatt tttatgatga
    19681 atgaatatag cccaaattac tatataggcg taatgtccgg cacgagcctt gatggggtgg
    19741 atattgcttt gctggatttt gcaaaaaatc caccgaaaat gaccgcttgt gatttttttc
    19801 caatgccgga ggaattacgg gcggatattt ctgcgttact taaaaccggc gaaaccaatc
    19861 tccaaaaact gggggaaatt gatcaccgtt taggcttgct ttatgcagaa acgatcaatg
    19921 cgtttttagc gaaaaatcag ctaaaagcag aagatattca agccataggt tgtcacgggc
    19981 agacggtgtg gcattcaccg gattctgagt atcccttcac cacccaaatt ggcgatatga
    20041 atttggtggc agagcaaacc ggtattacca ctatcgctga ttttcggcgt aaagatatgg
    20101 cagtaggtgg gcaaggggct ccgcttgtgc cggcatttca tcaagctatt tttagcgatc
    20161 caaatcgtct aacagtggtg ctgaatattg gcggcatcag caatatttcg gtgttaaatc
    20221 ccaattcccc tacagttggt tatgatgtag gagccggaaa tactttgatg gatagttgga
    20281 tcgaacaaca tcaaggcaaa agatacgata aaaacggcga atgggcgaga acgggcaagc
    20341 tcaatactga actgttagcg acgttattag acgagtcgtt ttttgctaaa gccccgccta
    20401 aaagcactgg gcgggagctg tttaatttaa cgtggcttgc aaaaaaattg gaaaatttga
    20461 ccgcttgtca gccacaagat gtgcaacgca cgctggcaga atttactgct caaagtattg
    20521 ccaatgagtt attacgcttt gaaagctctc tgcctcgctt gcttttagtt tgtggtggtg
    20581 ggtcaagaaa tccgttatta atggaacgga tttctgcttt acttcccgag tgggaggtta
    20641 caaccactac agaatttggt ttagatattg attatgtaga ggctgccgct tttgcttggt
    20701 tagcttacca gcgaatccat aatttaccca gtaatatgcc tagcgtgaca ggagcaaaaa
    20761 aagccgtgag tttgggcgtg atttaccctg cttagattaa gaaaatgtaa ggtaatacat
    20821 tcattactcc gagagtggta atagctaaaa ttaatttaga gcttccaaac attaagcctt
    20881 ggtttttacc attgtctaat ttcactagca tattagatag tgccgtattc gcatacacgg
    20941 taatagtggc aaaaatcact aaatagagaa atgcagtgat ggttgaaagc cattggagtg
    21001 cataaaacca aggcattatt tgaagtataa tcagtattgg tgcaatataa ataagtttat
    21061 gtactgtcag atttaatttt cgtgaaaaat cagattgcaa taacactgca attaaattat
    21121 tcacaaagaa tgagacagag actaattctg gtttatccag aagattggtg aatatatagg
    21181 ggaatataat ataaaatgca cttcctagac caagcggaat aaaaagtaat aaatgtacaa
    21241 aaataaattg ttcatttagt attcctttta atgcaccaaa tttaaagcgt tcgcattgcc
    21301 catttgcaca ttcatgtgtt ttgggtgcgg ttttaaacat aacgaatagc ataataattt
    21361 caatgcagca gctaatccaa ataagccatt ctgctttacg gtagaaaata aatggaatag
    21421 taagtaatgg tgctaccatc gctgacaggc ttgatacctt cagaaattta ccttgagcct
    21481 tagttttgag ttggtaacta tctccggtat cttgaatatt ggctaataag aatgctctcg
    21541 cattggtact aaataaggtg ctaccgagcc cgaagaaaaa ggtagcaagt aatagcagag
    21601 ggtaattttt tgttgtcagt aataaaatgt aagctagggc atcgagcaaa cagccaagca
    21661 tcatgatagg gtaacgtcca aaactatcac cccaagaacc tgcaaaaagg gaaaatgctt
    21721 ggctggtaaa gaccaacaaa gaaaatgcag ttgcaatttg gctggtttca aagcctttgt
    21781 agaatgtaag tagataaaaa atacgctttg cattgcagca taagcaagac cggaaaagaa
    21841 tttccgccat aaaatgatat tttggatatt catagataat atttaatgag aaaaatgatt
    21901 tctaatataa ctaaatttaa taaggaagca caatgagaaa aatcaaatta ttgcatgtta
    21961 ttttatcttt aatattaggg ctttctgcgg cagcgattgg aagttatccg gcaataaaag
    22021 agattggcga ggagcgggca aaaaaacatc aagatatatt agatttaaat agagaacttt
    22081 atagtgcaat ggtgaaccgt gatttttcgg ctttgcaaac attactaagc gatgattttg
    22141 agctttattc atcaacaggt gaagtgattg ataagcaaga atggatcaaa ttaatccaaa
    22201 agagagtatt ggaatttgat aaggttgatt tgctatctac cgattttcag gggaatcaat
    22261 catctggatt attaagagta aatggtgagt tttttgggat agagcgggat aatgtggtgg
    22321 tagaaatgaa tattcgaacc attgtgagaa atacagaacg ccaaattaaa tgtattgtga
    22381 ttaaagaggt ttaagtgtcg caactattga atgccttagg gcagatgatt accgaacaac
    22441 gtaatccgaa ttcgatgaat attgaccgcc tttcagcgtt agatatagtc caagtcatca
    22501 accaagaaga caaacaagta gcgattgcgg tggagcagtg cctgcctcaa attgcacaag
    22561 cggtggaaaa aattgtgcaa gcctttgaga aaggtggacg attggtttat gtcggtgccg
    22621 gcacgagcgg aagattaggg gtgcttgatg cttcggaatg tccgcccact tatggcgtaa
    22681 aacctgaaat ggtggttgga ttaattgccg gtggtgagcg ggcattacgt ttcccgattg
    22741 aaggggcgga agataatcgt gagcaaggca aagtagattt acaagcggtc aattttagcg
    22801 aaaaagatgt attggtcggg attgcagcca gtgggcgaac gccttatgtg ttaggggcga
    22861 tggaatatgc taagcaactt ggtgcgacca cggtgtcgat tgccagtaac ccaaattcgg
    22921 caatggcaca aattgcggat attgcgattg aaaccgttgt cgggccggaa gtgctaaccg
    22981 gttccagccg tatgaaatcc ggcacagctc aaaaattggt attgaatatg ctcactactg
    23041 cgtcgatgat tttaatcggt aaatgctacc aaaatttaat ggtagatgtg caagcaacta
    23101 atgaaaaact caaagctaga gcaattcgga ttgtgatgca agcgacagaa tgtgaacgag
    23161 aagtggctga agatacacta aaattagccg aaaataacgc taaattggca attatgatga
    23221 ttttaggtaa cttagataag cagaaagccg aagatttatt aagccaaaat caagggcgat
    23281 tacaaaaagc gttaaaatag aagtatgtct gctctcccct tgtgggagag agacagatca
    23341 atcattcatt cagaatgatt gatcagagag aggggaggca gatttaagca aataaatggc
    23401 gatgtttcac tagttcttct ttggttaaac tgaacacccc taagccaccg tgttcaaatt
    23461 ccagccagtt aaacggcaca ctggggaatt gttcgattaa atgcaccata ctgttgccga
    23521 cttcgcagac tagcacgcca tcatcattta agtaatcagc ggcttgagca agaatccgct
    23581 tggttaaatc taacccatct ttgcccgagc caagagctaa tgccggctcg tgatggaact
    23641 cttcgggcat atcgcctaag tcttcttcat caacataagg cggattagtc acaattaagt
    23701 cgtatttatc ttgcggtaaa ttgttaaaca gatcggaaca gatcgggaaa actcggtgag
    23761 acacctgatg acgctcaata ttaatttgag caacgtctaa ggcatcaagg gaaagatcaa
    23821 ccgcatcgac ttctgcttcc ggaaaacgct cggcacaggc aatcgcaata caaccgctgc
    23881 cggtacacat atctaaaatt cgatgtggct cgtttttgat aatgccttta aacccttttt
    23941 tgatcagctc accaatcggt gaacgtggca cgatcacacg ctcatcgaca taatattcac
    24001 cgccacaaaa ccaagccgag ttggtaaggt atgctacagg tttgcgagag cctaaacgct
    24061 cacagattaa attgatgata cgttctttct caactcgagt taagcgagag gaataaagtg
    24121 tttccggtac atcaaacggc aaataaagcc ctgccaacac taattgttgt gcctcgtccc
    24181 acgcattatc gtagccgtgc ccgtaataaa gatcagaggc attaaaatag ctataccccc
    24241 aacgcatcat atcttgaata gtgactaaat cattcacaat gggcagggcg gaaatttcat
    24301 caagaagttc ggtattatat ttaaacattg gcgtgccttt tttcgtattg ttaaaatggc
    24361 tgtgttatac cacattttgc tttgtgttag aatgtaaaaa tgaataagaa ggaaaagata
    24421 atgaacgata cgttattaga cgatgaattt gcattattca gagaggcagc aaaaggcacg
    24481 aaaaagctga cacaaaatac ctttgtgccg aaagcggaac ctcgcaagaa attgcaagaa
    24541 ttgcgagaat taaaagaaaa agctgataca gaattttatt tctcggatga atatgagcct
    24601 ttgctgaaag aagaaaatga aaaagtgaaa tatctgcgag aagatgttga tccttatatt
    24661 ctcaaacagt tacggcgagg cgatttccaa cccgagctgt ttttagatct gcatggttta
    24721 actcgagaaa aagcgaagaa agagttggca gcgttgattt tagcctgtga gcgggaaaag
    24781 gtatattgtg ccagtgtgat gaccggcttt ggcactcgca cgctcaaaga tcaaattcca
    24841 cgctggttgg ttcagcaccc aaaagtaatg gcgttgcacc aggccccgaa agagtggggc
    24901 ggagatgcct ctattctaat tttggttgag cagcctgaaa atcctgaaaa aatgtttggg
    24961 cgttaaaatt cggctgaatt gccaattttt tattaaaaac gaccgcttgt gtatcattaa
    25021 ggagaaatga tgtcagaaca aactttccac caacttacag cattattaga ttcgcatcga
    25081 gctcgctatc gagtggtagg acatccttct gccgggaaat cggaagaagt ggcaaaaatt
    25141 cgaggaacag aaatcgggca aggggcgaaa gccttagttt gcaaggtaaa aggcaatggg
    25201 gtaaagcaag ctgtgcttgc tatattacct gctgattacc aagcggattt aacaaaaatt
    25261 gcagagcatc ttggtgggtt aagagcctca cttgccagcc cggctgaagt ggcagaattg
    25321 accgattgtg tatttggggc aattccgccg tttagctttc accccgattt attattggtg
    25381 gctgagccaa gtctgtttga gcgttatgct gaattagcct ttaatgccgg tacgttggaa
    25441 cgttcgatta ttctgaatac cgaggattac caaaagatag ttaatcctgt gttggttcaa
    25501 ttcgccaaaa gctaggttat ggggcgaaaa actccacgat tttgccaata gcgatatcca
    25561 ttaatttagg gttagaaaaa gcgtgatctg ccccttcaat cgggagcagt tcaatcacgt
    25621 tattatcggc aaactgctgt gaagtgctta acgccaccat ttcatcttcc gtgccgtgca
    25681 gaattaaaat ttgctcggca taatccaaat aatcgttttg ggtaatatcg gttagctcat
    25741 caaaaaaggc ttggctgatt ttcatctggc gatcaaaccc ccacaaaatt tctcgccctt
    25801 tggcaagttt ttgccgttcc tcttctgaca agcggttcat taacgaatgg tagatttgta
    25861 ctgccggtgc ccgcaatgca attttccgaa acggattgcc tttttccgcc agatatttca
    25921 aggttaaata gccgccaaaa ctagtgccgt aggcatacag atttttagca ttcaggctgg
    25981 ttttggcata atcaatcacc agttgcagat aagtaatgga ttcctcaacc gagagttttt
    26041 tacgggcatc atcaccgtgg caagggaaat caaaggcaag ggcagcgtag cctttatatt
    26101 ttgagctaag gcgttcccca aatttggtat tcgatttcag atctttagac gaaccaaagc
    26161 cgtgcaacac aatcactaca ttctcgatct gctgcaattc tttttcataa aacagtttac
    26221 agcgaatgct gagaccctgt tcattaatct caaagcgttt ttccatttta tgttaagtat
    26281 tgaaattgaa gatgacaaaa ttttagcact atttgagtgc aagcggtcgg atctctcgca
    26341 aaatttgcaa attttccggt aaatttaacc gcttgtacta gccaaaattt aagcatattt
    26401 atagaataca gccttcgttt ttaaaaggag aaatgaaatg ttacaactta ttttagcgat
    26461 tttatgtagc gtgactgtag gcgtgttgat taaattcgcc cgccaaaaag ggattcgtat
    26521 cgaccaaagt attgcggtga actatatcgt gactaccacg atgacgtttt tattactaaa
    26581 accggattac caagggcagt cgattaccga tattattgct aataacccga cttcctatat
    26641 ttttgtgatt ttaagtatca cattgcctac tattttcttg gtgcaagcaa aagccctaga
    26701 gtttgccggt attatccgaa ccgatgcggc acaacgttta tcgctgttcc tgccgatttt
    26761 ggcggcattc accatttttg gtgagcaggt tacgcaaaat aaattaattg cacttctgtt
    26821 agctttcgtg gcactcggtt gcttattatg gaaaggcaat cagggaatgg aaaaaggcgg
    26881 aaaaattgcg attatcagcc ttgctctggt ttgggtaggc tatggcgtga acgatatttt
    26941 atttaaacag attgccaaat caggctctgc tttccctgtc accctttcga ttgcctttat
    27001 cggtgcaggc tgttttatgt ttatctattt gctacttaaa cgcactcaat ggcacgttcc
    27061 aagtgtctta accggcttat tgctaggcgt gttgaacttc ggcaatattc tgttctatat
    27121 tcaagctcac caagccatga aagatgaccc aactttagtc ttcactggaa tgaacttagg
    27181 ggtaatttgc ttaggcactt tcgttggagc attcttcttt aaagagaaaa tccacaaagt
    27241 gaactactta ggcgtggcga ttgcgattgt ggcgattatt tgcttgtttt attggcggta
    27301 aatatacttt ccaaaattaa gggcgaacat aatgttcgcc cttaattttt gagtaatata
    27361 gtgtaaaata ttcaataaat tcgaccgctt gttgtaccga ttcatatttc ataaaggaca
    27421 aaaaatgaag ctaaaagcct tatctacact cattgcctct tcaatgctat atagtcagag
    27481 ttttgctgta ccgccggaaa aaacagctga agagccacag ccctttttca ataaaaccga
    27541 atctgggtat gatttagtct ttaatgatca aaactatcgt ttaattgaaa cggaattaaa
    27601 tggcgagaaa atccaattcc gtgcctttga gcggattgtg tatgtgaaca atccgattga
    27661 gccagattac caaacaatta atctgtatgt gccggaagct tactacaata atggcgaaat
    27721 caatggctac caagcggata ccgccccgat cttcttgcca aattcagtgg ggggctatat
    27781 gccggcaaaa gcggcaagtt atgataaaaa aagacgtgac ggttcaccag gtacgatttt
    27841 ggtggcgtta tctaaaggct atgtggtggc aagtgtggga gcaagaggac gcacgctaca
    27901 aaaagaggga aaatacaccg gcaaagcacc ttctgccatt attgattcaa aatccgccgc
    27961 ccataaagcg tgtcggttcg agtccgacca ctggcaccac tcactatgta aaatttagtt
    28021 ttacatagtt tgctgtaagc atttgaattt tgaatcgttt gtgtaggatt gttcaaatca
    28081 tattcgcaaa tacacagtta ctacgatgta tccagccaat acatccattg ggattttccg
    28141 tccgaatttc agacgttcta gatcttacac attctttcaa gtttgtaaag cctgaaaatc
    28201 attttttaga ttgattttat ttgaaaaaat ctattttttc ttttaattca aatttagtca
    28261 aaacttcacc agatatttt
//
LOCUS       DANLZC010000015        26408 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.015, whole genome shotgun sequence.
ACCESSION   DANLZC010000015 DANLZC010000000
VERSION     DANLZC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 26408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26408
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.015"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            37..513
                     /gene="greA"
                     /locus_tag="PXE44_000505"
     CDS             37..513
                     /gene="greA"
                     /locus_tag="PXE44_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL5588048.1"
                     /translation="MKQIPMTVRGAEQLREELDFLKNVRRPQIIDAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIQEIEGKLGNAQIIDVTKMANNGKVIFGATVQLVNADTDEE
                     VTYRIVGDDEANIKEGLISVNSPIARGLIGKEVDDSVSITTPGGKVEFDIVGVEYI"
     gene            complement(569..985)
                     /gene="ndk"
                     /locus_tag="PXE44_000506"
     CDS             complement(569..985)
                     /gene="ndk"
                     /locus_tag="PXE44_000506"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL5588049.1"
                     /translation="MIQQTLSIIKPDATRRNLIGKILAQLEENGLTIKALKKVHLTRE
                     QAEGFYAEHKGKDFFEPLVEFMTSGSIVVSVLEGENAISHYRALMGATNPDNREKGTI
                     RDLYALSMRENSVHGSDSEESAKREIAYFFVPSEIV"
     gene            complement(995..3073)
                     /gene="metG"
                     /locus_tag="PXE44_000507"
     CDS             complement(995..3073)
                     /gene="metG"
                     /locus_tag="PXE44_000507"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL5588050.1"
                     /translation="MANQKRKMLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGN
                     EVHFVCADDAHGTPIMLNADKLGITPEQLIEKAKADHEADFASFNISFDNYHSTHSEE
                     NRQITTDIYKTLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
                     VCASTYSPMDLINPRSAVSGATPVVKESEHFFFDLPSFEGMLKEWTRSGSLQPEIANK
                     MQEWFESGLQQWDISRDAPYFGFEIPDAENKFFYVWLDAPIGYIASFKNLCDRAGIDF
                     DEFWQKDSTTELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSK
                     SRGTFIQANTYLKHLDPECLRYYYAAKLNDRIEDLDFNLDDFVQRVNSDIVNKLVNLA
                     SRNASFIAKRFEGKLAEKLDDEALFNEFTAQAETIANFYENREFNKAIRAIMELTDKA
                     NKYIDDKAPWVIAKEEGKEAELQAVCSMGIELFRVLMSYLKPVLPKLAERAEAFLQSE
                     LRWDNIATPLFGHTVAPFKSLFSRLEKKQIDAVIDETKALFAEANKKTENQTKGTQKV
                     EQQAVEKQENIAVEPISPEITIDDFAKLDLRAAKVLNCEAVPKSDKMLRFELDLGDHQ
                     RQVFSGIKAAYPNPEALIGRFVIVIANLAPRKMSFGMSEGMILSAGTGGANLFLLDAD
                     SGVQPGMQVK"
     gene            3285..4256
                     /gene="menC"
                     /locus_tag="PXE44_000508"
     CDS             3285..4256
                     /gene="menC"
                     /locus_tag="PXE44_000508"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDL5588051.1"
                     /translation="MRQVKLYRYQLPIQTGVVLRKQPLKERQGVLVCLQEQEKVGWGE
                     IAPLPSFSQESLEQAENQAVNWLKAWQKGEPETLEQLFPSVAFGLSCALAELNNELGE
                     QANNQSAVLCYGDLEKLHTAFSQHTNPIGKIKIGLNPEQEGEQANLLLHTFPNLRLRL
                     DANRLWSLEQAVKFAEKIANEHKPRIQFIEEPCQALELSRQFATQTDIAIAWDETVRE
                     ANFLVKKEPNLTAIIIKPTLVGSLEKCVNLIQQAQKQGLVAVISSSLESSLGLTQLAR
                     IAQQYTPNSVPGLDTLNLMQHQLLRDWHDSSLPLVGLESEFVREVVV"
     gene            4501..5202
                     /gene="rsuA"
                     /locus_tag="PXE44_000509"
     CDS             4501..5202
                     /gene="rsuA"
                     /locus_tag="PXE44_000509"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDL5588052.1"
                     /translation="MRLDKFIAENTGLTRSQAAKVLKSGVVTVNGNIEKSGAAKISQE
                     DEIYYEDQKLEWVEEGQYFMLYKPQGYICSHDDGEYPTVFQFFDYPLMTKLHTAGRLD
                     VDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVEDFYAQRLAEGILLRGEKEP
                     TLPAQLEILDDYNVNLTISEGRYHQVKRMFAALGNKVEALHRWRIGDVVLDERLEEGE
                     FRSLTSEEIASFNGK"
     gene            5192..6373
                     /locus_tag="PXE44_000510"
     CDS             5192..6373
                     /locus_tag="PXE44_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDL5588053.1"
                     /translation="MESRRPSLSFFIILGMMAMLPPLAIDMYLPSFLDIARDLAVSQE
                     KVQTTLAVFTLGFGAGQLFWGPMADSFGRKIIILIGLVGSAIAAYFLTQVQNIETFYL
                     LRLVQGLCAAAPAVVLGALVRDLFERNLFAKLMSVIMIISMLAPLLAPIVGGYIAKYF
                     HWHSIFYVLVAMGILCVVLISWKIPETLAPEKRLPLSFGKTLSNFAILLKHRPTLGYV
                     LIGGLTFAGIFCFLTSGSIVYIGLYGVSQENFGYFFGLNMIVMVTMTAINSKMVVKVG
                     SETMLRIALAMQLLAGIWLALVAIFDLGFWAMAIGIPLYIGMLSTIGSNASAAILEQF
                     PQMAGTANGVAGTARFGVASLVGAGLSHIAVTSAAPMLLAMALCVMLAGVVYYFLCYK
                     R"
     gene            complement(6513..7832)
                     /gene="rlmD"
                     /locus_tag="PXE44_000511"
     CDS             complement(6513..7832)
                     /gene="rlmD"
                     /locus_tag="PXE44_000511"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL5588054.1"
                     /translation="MAIFYSEKSAKKPIKSPACQKATIQSLDYQGLGIAKINGKTWFI
                     ENALPDEQVEFKVLEEKRQYGRGQAVKILKKSANRRMPSCELYGQCGGCQMQHISLEL
                     QRKAKQQALFQRLQKLQTHAIDVQPMIIGNDKHYRRRAKLSIAIQQGKAVIGFRLQNS
                     NQIIPLAHCEVLVEPLANLLPKLKHLIENWQQPKKLGHIELVSADNTIALFFRHLGAL
                     SAQDNHNLRQFATQEQLSLFVMTSENHIEHLCGERPYYQIQGLKLHFSIRDFIQVNAT
                     LNEKMVEKALEWLELSNQDRVLDLFCGMGNFTLPIAERAKSVVGVEGVEPMVQQAREN
                     AVTSGLKNVEFYQTNLDEPFSDKPWAKEPFNKVLLDPARNGALFCLDHLTSLNPERIV
                     YVSCNPATLVRDAEKLLQLGYRLEKAAMIDMFPHTGHLESISLFLKS"
     gene            7917..9242
                     /locus_tag="PXE44_000512"
     CDS             7917..9242
                     /locus_tag="PXE44_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HDL5588055.1"
                     /translation="MISSVWNERYLADKKREQDHRSPFQRDRARLLHSEAFRCLQAKT
                     QIHAVGEDDFYRTRLTHSLEVAQIGSSLREKLLADVEGFQAVCSEQEFAKNMANLTAL
                     LPDHSLVESLCFAHDLGHPPFGHGGEMALNYKMYAFGGFEGNAQSFRIMTQLEPYTET
                     AGMNLTRRTLLGVMKYPALLSQTSPIEPAEMSEARHINLHHFPQSKGIFDDNNHFFEW
                     VLAPLSEKDRRQFCTVQPNQDPKQPNKTQYKSLDCSIMELADDIAYGVHDLEDAIVGG
                     MVTPQSWQNAEKLLAECQSDWVKQRLPEIREKLFSQHRYERKDVIGALVNHFITNVRW
                     KALPEFDEPLLRYNAYLPESVACVLKILKDFVYQYVICDAKTQRVERKGQRILMELFD
                     ILSSDPMRLLPNNVRERWKNAEESKRARVICDYLAGMSDGQAFKLFESL"
     gene            9363..9662
                     /locus_tag="PXE44_000513"
     CDS             9363..9662
                     /locus_tag="PXE44_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588056.1"
                     /translation="MRFLPSEIYQPRGELVKADRQGNGEFEVEYRVSGNDVRGLAKSA
                     IAHAKRKGFHLVESDIHRDDADLKFKRGDQELDIEIEVKGRNRIEYKADLDLDKN"
     gene            9823..12735
                     /gene="rapA"
                     /locus_tag="PXE44_000514"
     CDS             9823..12735
                     /gene="rapA"
                     /locus_tag="PXE44_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDL5588057.1"
                     /translation="MFLVGQRWLSESENNLGLGIVTSVDHRTVTINFPAAEEERVYAI
                     AVAPLTRVQFQAGDRINSIDGWQLEVSEVVENQGAVIYLGKRVDNGEEAVLPEMLLDH
                     KISFSKPQDRLFSAQIDRSDRFALRFNALQHQQAQYQSPLRGLRGIRASLIPHQLHIA
                     KEVGQRLAPRVLLADEVGLGKTIEAGMILQQQLFSGRAERVLVLVPESLQHQWLVEML
                     RRFNLKFSLFDEERCSDFDKQDENGDDVSENPFESEALVITSLNWLETSPQRANQVLA
                     ADWDLLIVDEAHHLEWSETESSIGYQLVERLSQQIPAVLLLTATPEQLGQESHFARLA
                     LLDSDRFYDYAEFVQEQAQYKPVADAVSSLLSDKPLSNDEQNAIAELLSEQDTEPMFR
                     AINSKKSDENDRLQARQELISELIDRHGTSRVLFRNTRQGVKGFPHREYHQISLEMPK
                     QYQNALKVMGMMGSVSQSDLLYPEYLFQRMNPNAKWAEFDPRIDWLITFLKNHRDEKV
                     LVICKQADTAIALEQVLREREAIRSAVFHEKMSIVERDKAAAYFAQQEEGAQVLISSS
                     IGSEGRNFQFAKDLVLFNLPDNPDLLEQSIGRLDRIGQKNDIQIYVPCFNLSAQQMLA
                     EWYHKGLNAFEETCPMGTAIFRELGEELQQFLQKPELSAQFDDFVAKTYKRQQYLKAE
                     LEKGQDRLLELNSNGGEAAQQLANDIAKEDNNPQLVNFALSLFDVIGVEQEDLGEQSI
                     VISPTGHMLVPDFPGLSEDGTTITFNRELALMREEVEFLTWDHPMIRNGIDLITSGDI
                     GKSAISLLVNNKLPAGTLLLEALYVVEAQAPKGLNLTRFLPPTPIRVLVDNKGNNIAP
                     QVSFAGLERQLKPVNKQMANKIAKMARADIEKLIKASEKAVEPQVQQVIETAKFEANS
                     KLKAELHRLESLKMVNKNIRADEVEALEQQLNASLAQLETANYRLDSLRVIVSNKA"
     gene            12862..13158
                     /locus_tag="PXE44_000515"
     CDS             12862..13158
                     /locus_tag="PXE44_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5588058.1"
                     /translation="MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKV
                     VLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRLISVRRSRKKEISYYEKYH"
     gene            13142..13384
                     /locus_tag="PXE44_000516"
     CDS             13142..13384
                     /locus_tag="PXE44_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDL5588059.1"
                     /translation="MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFP
                     NWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR"
     gene            13396..14064
                     /gene="rluA"
                     /locus_tag="PXE44_000517"
     CDS             13396..14064
                     /gene="rluA"
                     /locus_tag="PXE44_000517"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDL5588060.1"
                     /translation="MALIEYNPPLEPFLDEVYRDNHILVINKPSGLLSVPGNRPEYYD
                     SAMTRVQQKYGFTEPAHRLDMATSGILLFALSKAAEKELKRQFREREPKKHYIALLWG
                     KLGEKVGDKGEMNFPLICDWENRPRQKICYERGKKAITHYEVLAHYPNNIARVKLTPI
                     TGRSHQLRVHSLALGHPIIGDKFYANPLAKSLSPRLCLHAESLTITHPITRELMTFTA
                     EPTF"
     gene            complement(14042..14617)
                     /gene="ampD"
                     /locus_tag="PXE44_000518"
     CDS             complement(14042..14617)
                     /gene="ampD"
                     /locus_tag="PXE44_000518"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDL5588061.1"
                     /translation="MPNLKIEQGCLTGQKQVPSPHFSQRPDQEDISLLVIHYISLPPE
                     EFGGNFIDLFFQGKLEPTAHPYFEEIKDLRVSAHCLINREGEITQYVNFNDMAWHAGV
                     SCFEGREKCNEYSIGIELEGSNNQPFTQAQYQSLANLTKAIQQSYPQITDERITGHCH
                     IAPERKIDPGQYFDWAYYKHLLNEAKKSAQQ"
     gene            14677..15519
                     /locus_tag="PXE44_000519"
     CDS             14677..15519
                     /locus_tag="PXE44_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HDL5588062.1"
                     /translation="MLSYRHSFHAGNHADVLKHIVQLLILRALKEKEKGFLYLDTHSG
                     VGRYSLLSFESEKTGEYLEGVARLWERDDLPEEVAFYIDELKKLNRGGKLKYYAGSPL
                     LAVQQLREQDRAILTELHPNDFPLLRQEFAKAKNVQTKRENGFQQLKSALPPKERRGF
                     VLIDPPYELKEDYELVVKAIEEGYKRFATGVYAIWYPVVLRQHTKRIVRGLMDTGIRK
                     ILQIELAVRPDSDQRGMTASGMIVINPPWQLETQMKKILPYLTEVLVPEGTGSWKVEW
                     ITPE"
     gene            15642..16283
                     /gene="pyrE"
                     /locus_tag="PXE44_000520"
     CDS             15642..16283
                     /gene="pyrE"
                     /locus_tag="PXE44_000520"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL5588063.1"
                     /translation="MQQYKHDFIEFALSRNVLKFGEFTLKSGRISPYFFNAGLFNTGR
                     DLAKLGEYYAKAIQASGIEYDVLFGPAYKGIPIATTVAVALANLFDVDKPCCFNRKEA
                     KDHGEGGNLIGSPLTGKILLVDDVITAGTAIRESMEIINANNAQLAGVLIALNRKEKG
                     KGELSAIQEVERDYGCQVFSIVDFDDLVAFIETSPQYAPYLPKMREYREKYGV"
     gene            16457..17546
                     /locus_tag="PXE44_000521"
                     /pseudo
     CDS             16457..17546
                     /locus_tag="PXE44_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607068.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     gene            complement(17611..18801)
                     /gene="metC"
                     /locus_tag="PXE44_000522"
     CDS             complement(17611..18801)
                     /gene="metC"
                     /locus_tag="PXE44_000522"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDL5588064.1"
                     /translation="MSNYKNLSTKLVHTGRKKRNTQGAVNPVIQRASSLVFETLEEKK
                     HATQNRYKGALFYGRRGTLTHFSLQEAMCELEGGAGCYLYPCGAAAVTNAILAFVEQG
                     NHVLMTGAAYEPTQEFCNALLKRMGVSTTYYDPMIGEGLKALVQPNTKVLFLESPSSL
                     TMEVPDIPTLVRVAREINPEIVIMIDNTWSAGVLFPALEYGIDISIQAGTKYLVGHSD
                     VMIGTAVSNARCWDKLREGSYLMGQMVDADSAYITARGLRTLGIRLKQHEQSSIAVAN
                     WLAQRPEVKAVYHPALESCPGHEFFKRDFLGSSGLFSFELCEKLSNDKLANFLEHFEL
                     FSMAYSWGGFESLILANQPEEIAKIRPAIERKLTGTLIRIHVGLEAVEDLIADLEQGF
                     NRIR"
     gene            complement(18860..19399)
                     /gene="dsbB"
                     /locus_tag="PXE44_000523"
     CDS             complement(18860..19399)
                     /gene="dsbB"
                     /locus_tag="PXE44_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDL5588065.1"
                     /translation="MLYFFKNLSQSRSAWFLLMLSCLILEGTALYFQHGMGLVPCVMC
                     IYERVALFGILFAGILGFLGNRYAIIRYLAILIWLGSAFKGLVLSIKHHDYQVNPSPW
                     NQCEFKVNFPQTLPLDQWLPQVFAPGPVNCSQSQWEMFGFSMAQWLIFTFAVYLIVAV
                     IVLISQFKRSRQRNNFMFR"
     gene            complement(19462..21003)
                     /gene="nhaB"
                     /locus_tag="PXE44_000524"
     CDS             complement(19462..21003)
                     /gene="nhaB"
                     /locus_tag="PXE44_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HDL5588066.1"
                     /translation="MSSIDALFKSFLGKSPEWYKLCILAFLIINPLIYFFINPFVAGW
                     FLVAEFIFTLSMALKCYPLQPGGLLAIEAVVIGMTNPHHIKEEIMANFDVILLLMFMV
                     AGIYFMKQLLLYIFTKLLVAIHSKKILSLSFCLSAAFLSAFLDALTVIAVIISVGTGF
                     YGVYHKVASGNNFEDSTDITNDEKIISNKEILEQFRGFLRSLLMHAGIGSALGGVMTM
                     VGEPQNLIIASQAEWGFIEFLLRALPVSLPVLICGIATCFLVEKYKLFGYGDRLPSKV
                     WVVLARYSQLKEQKMTKQDRLKMLVQAIAGIWLIIGLALHLADVGIIGLTIIIICTAF
                     CGITDEHTLGKAFQESMPFTALLIVFFSVVAVIIDLKLFEPIIAFVLSAEPHSQLSLF
                     YIFNGLLSMISDNVFVGTVYINEAKTALDAGIIDREQFDLIAVAINTGTNLPSVATPN
                     GQAAFLFLLTSSFAPLIKLSYGRMVYMALPYTIVLSIVGFLGLEFLLPSMTEVMINWG
                     WFITR"
     gene            21160..21885
                     /gene="fadR"
                     /locus_tag="PXE44_000525"
     CDS             21160..21885
                     /gene="fadR"
                     /locus_tag="PXE44_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDL5588067.1"
                     /translation="MGHSDILKAQSPAALAEEYIVKSIWNNHFPAGSDLPAERELAER
                     IGVTRTTLREVLQRLAREGWLSIQHGKPTRVNDIWETSGPNIIGTIIKLDKSYLPVVI
                     ANVVSLRTRMAEAYIPEAIYLAPEESAKLFDGLDELENTAAAYAEFDYKLFRQFTFLA
                     NKPVYALILNSFKSMYHQVAKIFFADENNRQLTLSFYRSLLCTCLAKDSEKAAACMAK
                     NRQSSGEIWQKLSANIPESFGQL"
     gene            21900..22970
                     /locus_tag="PXE44_000526"
     CDS             21900..22970
                     /locus_tag="PXE44_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL5588068.1"
                     /translation="MNEIIFSLEPQDNARLQSLCGAFDEHLALIEKSFNLIIARTGFN
                     FTIQSEDDNHYSATLVQNSVKLIKQLYNETAPIKGKIKELDLEDIHLAIQESRMLLQN
                     QWQAGSQGEIAIKTKRGVIKPRSEHQQAYLRNILSHDISFGIGPAGTGKTFLAVAAAV
                     ESLERQEIRRILLTRPAVEAGEKLGFLPGDLGQKIEPYLRPLYDALFEMLGFEKAQKL
                     MERGVIEIAPLAYMRGRTLNDAFIILDESQNTTTEQMKMFLTRIGFNSKAVITGDITQ
                     VDLPRSQKSGLKHAMEVLKEVPELSFNFFDSKDIVRHPVVAKIVQAYDAWEAVDEERK
                     EQRKLEKLALEQEKILAQAEML"
     gene            complement(23096..25102)
                     /gene="tkt"
                     /locus_tag="PXE44_000527"
     CDS             complement(23096..25102)
                     /gene="tkt"
                     /locus_tag="PXE44_000527"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL5588069.1"
                     /translation="MTERKVLANAIRFLSMDAVQKANSGHPGAPMGMADIAEVLWRDF
                     LKHNPTNPKWADRDRFVLSNGHGSMLIYSLLHLTGYDLSIEDLKQFRQLHSKTPGHPE
                     YGYAPGVETTTGPLGQGITNAVGIAIAEKTLAAQFNREGHKIVDHYTYAFLGDGCLME
                     GISHEACSLAGTLGLGKLIAFYDDNNISIDGHVDGWFTDDTAQRFESYGWQVIRNVDG
                     HNPEQIKFAIENAQAETERPTLIICKTIIGYGSPNKSNSHDCHGAPLGDAEIQAAREF
                     LGWEHAPFVIPAEIYTEWDAKAKGTLAEKDWNAKFAAYEAAYPELAAEFKRRYAGELP
                     ANWEAESKAFIEKLQANPATIASRKASQNAIEAYAPILPEFLGGSADLASSNLTLWSG
                     SKPIRAEHNVDGNYINYGVREFGMSAIMNGIALHGGFIPYGATFLMFYEYAHNAVRMA
                     ALMKQRALFVYTHDSIGLGEDGPTHQPVEQTASLRQIPNLETWRPADQVESAVAWKAA
                     VERKDGPTALIFTRQNLAQQQRTAEQLANISRGGYILRECCEKGSCPDLILIATGSEV
                     DLAMKAADVLDAEGVKVRVVSMPSTNVFDKQDEAYRESVLPRSVTKRVAIEAQLADFW
                     YKYVGFDGRIVGMNSFGESAPADQLFKLFGFTVENVVAKAKEIL"
     gene            25465..25677
                     /gene="rpmE"
                     /locus_tag="PXE44_000528"
     CDS             25465..25677
                     /gene="rpmE"
                     /locus_tag="PXE44_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDL5588070.1"
                     /translation="MKQGIHPEYKEITATCSCGNVVKTRSTVGKDLNLDVCGNCHPFY
                     TGKQRVVDTGGRVERFNKRFSIPSSK"
     gene            complement(25732..>26408)
                     /locus_tag="PXE44_000529"
     CDS             complement(25732..>26408)
                     /locus_tag="PXE44_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HDL5588071.1"
                     /translation="SIYNLDTVKNYDFTTVFPKQVHFADYLNYAIFADFLEKNQVNYN
                     LNIPNELSSFANTLWQEKKIRLLLNPQGSMREISTQELSELLSMIDKRYVQHISCLLT
                     NTTNSAEYLSKIKAPFEISLAPNSDILGYCALVNSADIVIAVDGGGVHISCALGKKLL
                     SFYANHPANIARWYPKPKKDVETFMVIADKWTEDNNDTRGFSLVAPAKWLNHQFAKLI
                     EKYTAC"
BASE COUNT         7748 a         5206 c         5898 g         7556 t
ORIGIN      
        1 cgtaaaccga taatttttag taaggaaata gatttaatga aacaaattcc tatgacggtg
       61 cgtggtgcag agcaactgcg tgaagaattg gattttttaa aaaatgtgcg tcgcccacaa
      121 attattgatg cgattgcaga agcccgtgaa cacggtgatt taaaagagaa cgcggagtat
      181 cacgcagccc gtgaacaaca aggtttctgt gaggggcgta ttcaagaaat tgaaggcaaa
      241 ttaggcaatg ctcaaattat tgacgtcact aaaatggcaa acaacggcaa agtgattttt
      301 ggggcaacgg tgcagttagt gaatgctgat acagatgaag aagtcactta ccgcattgtg
      361 ggtgatgatg aagccaatat taaagagggc ttaatttcag ttaattcccc gattgctcgt
      421 ggtttaatcg gcaaagaggt ggacgattca gtcagcatta ccacaccggg tggcaaagtt
      481 gagtttgaca ttgttggcgt agaatatatt taatctacgc tagggcgaat tacaatagtg
      541 gggcgaaaaa attttcgccc ctactttatt aaacaatctc actcggtaca aagaagtagg
      601 caatttcacg ttttgccgac tcttcggaat ctgaaccgtg taccgaattt tcacgcatac
      661 ttaaggcata taaatcgcga atggtgcctt tttcacggtt atccggattg gttgccccca
      721 ttaatgcacg ataatggcta attgcatttt ccccttccag tacagaaacg acaatagatc
      781 ctgatgtcat aaattccact aacggctcaa agaaatcttt gcctttgtgt tccgcataaa
      841 atccttccgc ttgctcacgg gtaagatgta ctttttttag agctttgata gtcagcccgt
      901 tttcttccaa ttgagccaaa attttgccga ttaaattacg gcgtgtagca tccggcttaa
      961 taatagaaag cgtttgttga atcatcatct tctcctattt aacttgcatt cccggctgaa
     1021 caccgctgtc tgcatctaat aagaacaggt tcgcaccgcc tgtgccggca gataaaatca
     1081 ttccctccga cataccgaac gacattttgc gtggagcaag gttggcaatt acaatcacaa
     1141 aacgcccgat aagtgcttcc ggatttgggt aggccgcctt aatgcctgag aatacttggc
     1201 gttggtggtc gcctaaatcc agctcaaaac gtaacatttt gtctgatttt ggcacagcct
     1261 cgcagttcaa tactttggct gcacgtaaat ctaatttcgc gaaatcatca atcgtaattt
     1321 cagggctgat tggctcaact gcaatatttt cttgtttttc gaccgcttgt tgttccactt
     1381 tttgtgttcc tttggtttgg ttttcggttt tcttattcgc ttcggcaaat aaggctttgg
     1441 tttcatcaat cactgcatca atctgttttt tctcaaggcg tgagaatagc gatttaaacg
     1501 gtgccacggt atgcccgaat aatggggtag cgatattatc ccaacgcaat tcgctttgta
     1561 agaatgcttc ggcacgttct gccagttttg gtaacaccgg ttttaggtag ctcatcagca
     1621 cacggaataa ctcaatgccc attgaacaaa ccgcttgtaa ttcagcctct ttgccttctt
     1681 ctttggcgat gacccaaggg gctttgtcgt caatgtattt attggcttta tcggttaatt
     1741 ccataatggc acgaatagct ttattaaatt cacggttttc atagaaattc gctatggttt
     1801 ctgcttgagc ggtaaattca ttaaataacg cttcgtcatc taatttttca gctaatttgc
     1861 cctcaaagcg tttagcgata aaactggcgt tgcgagaagc aagattcact aatttgttta
     1921 cgatatcgct gtttacacgt tgcacgaagt cgtctaaatt aaaatctaaa tcttcaatgc
     1981 gatcgtttaa ttttgccgca tagtaatagc gtaagcattc cggatcgaga tgttttaaat
     2041 aggtgttggc ttgaatgaat gtaccacgag atttcgacat tttcgccccg tccactgtca
     2101 cataaccgtg agcaaacaca ttggtcggtt tgcggtagcc gctgccttcc aacattgccg
     2161 gccaaaacag gctgtggaag taaacaatgt ctttaccgat gaagtgataa agctcggtgg
     2221 tggaatcttt ttgccaaaac tcatcaaaat caatgcctgc acggtcgcat aaatttttaa
     2281 acgatgctat ataaccaata ggggcatcta accaaacgta gaagaatttg ttttctgcgt
     2341 ccggaatttc aaaaccgaaa taaggggcat cacggctgat gtcccattgc tgtaaaccac
     2401 tttcaaacca ttcttgcatt ttattggcaa tttcaggttg gagtgagccg gaacgagtcc
     2461 actctttcaa cattccctca aagcttggca ggtcaaaaaa gaaatgttct gattctttta
     2521 ccaccggagt tgcaccggaa actgccgagc gagggttgat taaatccatc gggctatagg
     2581 tagaggcaca tacttcacag ttatcgccat attgatcttc ggctttacat ttcgggcagg
     2641 tccctttcac aaagcgatcc ggcaagaaca tatttttttc tgggtcgaac agttgtgaaa
     2701 tcactttgct tttaataaaa ccgttcgctt ttaaggtttt ataaatatcg gtagtgattt
     2761 ggcggttctc ttcgctgtgg gtggaatggt aattatcaaa gctgatatta aaactggcaa
     2821 aatccgcttc gtgatcggct ttggcttttt cgattaactg ctcaggggta atgcctaatt
     2881 tatcggcatt aagcataata ggcgtgccgt gggcatcatc agcacagaca aagtggactt
     2941 cattgccacg catacgttga aaacgcaccc aaatatctgc ttgaatatgt tcaagcatat
     3001 gacctaaatg aattgcaccg ttagcatagg gcaaggcaca agtaacaagc atttttcgtt
     3061 tttggttagc cattcttttt ctcattaaaa ttagggtaaa aattgccgtc tattttagcc
     3121 caaaagtggc aggtttacca gttggcgtga atttattaga gctttgggct aacaagcggt
     3181 agaatatgat gcgctagagg cgaaacattt ttcgcctcaa tttcccaaat gatgatgtgg
     3241 gcgaaagatg tttcgcctct aaacctattt tggataaaaa tagtatgcga caagtaaagt
     3301 tataccgcta ccaactcccc attcaaaccg gtgtagtgtt acgcaagcaa cctttgaaag
     3361 aaaggcaagg ggtgttggtg tgtttgcaag aacaggagaa agtcggctgg ggcgaaattg
     3421 caccgcttcc aagttttagc caagagagcc tagaacaagc tgaaaaccaa gctgtgaatt
     3481 ggttaaaggc gtggcagaaa ggagagcctg aaacgttaga gcagcttttc ccgtctgttg
     3541 cctttgggct aagctgtgcg ttggctgaac tgaataatga attaggcgag caagcgaata
     3601 accaatcggc ggtgctgtgc tatggcgatt tggaaaagtt acacacggca tttagccaac
     3661 atacgaaccc aatcggtaaa attaaaatcg gtctcaatcc tgaacaggaa ggcgagcagg
     3721 caaatttgct gttgcatact tttccgaatt tacgcctaag gcttgatgcc aaccgtcttt
     3781 ggagcttgga acaagcggtc aaatttgctg aaaaaattgc aaatgaacat aaaccccgta
     3841 ttcaatttat cgaagaacct tgccaagcgc tcgaactttc ccgccaattt gccacacaaa
     3901 ccgacattgc gattgcgtgg gacgaaaccg tgagagaggc gaattttttg gtaaaaaaag
     3961 agccaaattt gaccgctatt atcattaaac ctacgctggt tggttcgctt gaaaaatgcg
     4021 tgaatttaat tcaacaagca cagaagcaag gattagtagc ggtgattagt tcaagtcttg
     4081 agagcagttt gggcttaacc caactggctc gtatcgccca acaatacacc ccaaacagtg
     4141 tgccggggtt ggatacgtta aatttaatgc aacaccaatt attgcgagat tggcacgact
     4201 ctagcttgcc gttggtcggg ctagaaagcg aatttgttag ggaagttgtg gtataatctc
     4261 ccaattaaac actctctccc cttgtgggag agagaccgct tatgagcgtt cagcgaaata
     4321 agcagagaga ggggaagcgg tcatttttct aaaaaagttt gcacatttcc cctctccctg
     4381 atttttcttc tggacgaaga cgtatttccc ccctctccct gatttttctt ctagacgaag
     4441 aaaaatctgt ccctctccca cgaggggaga gggtaaagta atttatggta taaaaatagt
     4501 atgcgtttag acaaatttat cgccgaaaat accggtttga cccgttcaca agcggctaaa
     4561 gtgctgaaaa gtggtgtggt gacggtaaac ggcaatattg aaaaaagtgg tgcggcaaaa
     4621 atcagtcaag aagatgaaat ttattacgaa gatcagaaat tagagtgggt ggaagagggg
     4681 cagtatttta tgctctataa gccacagggc tatatttgtt cacacgatga tggtgagtac
     4741 ccaacggtgt ttcaattctt tgattatccg ttaatgacta aattgcatac cgcagggcgg
     4801 ttagatgtgg ataccacagg gctggtgctg ttaaccgatg acggcaaatg gtcgcaccgt
     4861 attacctcgc ccaagcacca ttgtgaaaag acatacttag tgacgcttgc tgatccggtg
     4921 gaagattttt atgcacagcg attggcagaa ggaattttat tgcgtggcga gaaagagcca
     4981 accttgccgg ctcaacttga aattttagac gattacaatg tgaatttaac tattagtgag
     5041 gggcgttatc atcaagttaa gcgaatgttt gccgcccttg ggaataaagt cgaggcattg
     5101 caccgctggc gaattggcga tgtggtgtta gatgaaaggt tggaagaagg tgaattccgc
     5161 tcgctcactt cagaggagat agccagtttt aatggaaagt agaagaccaa gcctgagctt
     5221 tttcatcata ttaggtatga tggcaatgtt gccaccatta gccattgata tgtatttgcc
     5281 ctcattttta gacattgccc gtgacttggc ggtttcgcaa gaaaaagtgc aaaccacctt
     5341 ggcggtattt accctaggtt ttggggcagg gcaactattc tggggaccga tggcagacag
     5401 ctttgggcgg aaaatcatta ttttaatcgg attagtcggc tcggcgattg ccgcttattt
     5461 tctgacccaa gttcagaata ttgaaacatt ttacctgcta aggttagtac agggcttatg
     5521 tgcggcagcc ccggcggtgg tgctgggagc attagtgaga gatctgtttg agcgtaattt
     5581 atttgccaaa ttgatgtcgg ttattatgat tatttctatg cttgccccat tgcttgcacc
     5641 gattgttggc ggttatattg ccaaatattt ccactggcac tcaattttct atgtgttagt
     5701 ggcgatgggg attttgtgcg tggtattaat tagttggaaa atccctgaaa cattagcccc
     5761 cgaaaaacgc ctgccgttaa gttttggcaa aactttaagc aattttgcca tcttactgaa
     5821 acataggcct actcttggtt atgtgctgat tggcggctta acctttgcag gtattttctg
     5881 ttttttaacc tcaggctcga ttgtctatat tgggctttat ggcgtgtcgc aagaaaattt
     5941 cggctatttc ttcgggctga atatgattgt gatggtgacg atgacggcga ttaacagcaa
     6001 aatggtagtg aaagtcggct ctgaaacgat gttacgaatt gctttagcta tgcaactgtt
     6061 agccggcatt tggctggctt tagtggcgat ttttgattta ggcttctggg caatggcaat
     6121 cggtattccg ctttatatcg gaatgctttc caccattggt agtaatgcca gtgccgccat
     6181 tttagagcaa tttccacaaa tggcaggcac ggctaacgga gtagcaggta cggctcgttt
     6241 tggtgttgct tcccttgttg gagcagggct ttcacatatt gctgtaacca gtgctgcccc
     6301 aatgcttttg gcgatggcat tgtgcgttat gttagccggt gtggtgtatt attttctgtg
     6361 ttataagcga taactaggtt ggggttagct tgcgaaaacc caacaaattt ctacaagcgg
     6421 ttaataatag tacatttttt gcaaattttt aggcagaatt aaccgcttgt gtggtacgag
     6481 cgtggacgct cgcaccatcg gggcaatatc tactaagatt ttaaaaacag cgaaatagat
     6541 tccaaatgcc cggtgtgggg gaacatatca atcattgccg ctttttctag cctgtagcct
     6601 agttgcaaca gtttttcggc atctcgcacc agtgttgccg gattgcaaga gacataaaca
     6661 atccgctcag gattgagtga tgtcaagtga tctaggcaaa atagtgctcc gttgcgtgcc
     6721 gggtcgagta gtaccttgtt aaacggctct tttgcccaag gtttgtcaga aaacggctca
     6781 tctaaattgg tttggtaaaa ttccacattt tttagaccgc ttgtcaccgc attctctcgg
     6841 gcttgttgca ccattggttc taccccttcc acaccgacca ccgatttcgc tcgctcagca
     6901 ataggcaagg taaaattccc cattccacag aagagatcta acactcgatc ttggttggat
     6961 aattccagcc actcaagggc tttttccacc attttttcat tgagcgtggc attcacctga
     7021 ataaaatccc gaattgaaaa atgcaacttt agaccctgaa tttgataata cgggcgttca
     7081 ccgcacagat gctcaatgtg attttcgcta gtcatcacaa aaagagataa ttgctcttgt
     7141 gtggcgaatt gacgcaaatt gtggttatct tgcgccgata atgcccctaa atggcggaaa
     7201 aatagggcaa tggtattgtc cgcactgacc agctcaatat gccccaattt tttcggctgt
     7261 tgccagtttt caatcaggtg ttttaatttt gggagcaaat tggcaagcgg ttcaaccagc
     7321 acttcacagt gtgcaagcgg tataatttgg ttggaatttt gcaaacgaaa gccgattacc
     7381 gccttgcctt gctggatcgc aatgcttaat tttgcccgac ggcggtagtg tttatcgttg
     7441 ccgataatca tcggctgaac atcaatagcg tgggtttgca atttctgtaa gcgttggaat
     7501 aaagcctgtt gttttgcctt acgttgcagt tcaagcgaaa tatgctgcat ttggcagcca
     7561 ccgcattgcc cgtaaagttc gcagcttggc attcgccgat ttgcagattt tttaagaatt
     7621 ttaaccgctt gtccacgtcc gtattggcgt ttctcttcca acactttaaa ttccacctgc
     7681 tcatcaggca gggcattttc gataaaccaa gttttgccgt tgattttggc aatccctaag
     7741 ccttgataat ctaacgattg aatagttgcc ttttgacaag cgggtgattt tatcggtttt
     7801 tttgccgatt tttcagaata aaaaatagcc attgttttca gatgaattgt tatgatataa
     7861 agtgtcgaaa tggggagatt atataagaaa agcagttggc ggaaaatgga ggcaatatga
     7921 tttcatcggt ttggaatgaa cgttatttgg cggataaaaa acgggaacaa gatcaccgct
     7981 cgccttttca gcgggatcgg gcaaggttat tgcattcgga ggcttttcgc tgtttgcagg
     8041 cgaaaacgca aattcacgcc gtgggggaag atgattttta tcgaacccgt ttaacccatt
     8101 cgttggaagt ggctcaaatc ggtagcagtt tgcgagaaaa attattagcg gatgtggaag
     8161 gctttcaagc ggtctgttcg gagcaggaat ttgcaaaaaa tatggcaaat ctgaccgctt
     8221 tattgccgga tcatagcttg gtggaatccc tttgttttgc ccacgatctc gggcaccctc
     8281 cgttcgggca cggtggtgaa atggcgttaa attataaaat gtacgccttt ggcggcttcg
     8341 agggtaatgc tcaatccttt cggattatga cccagcttga gccttacacc gaaacagccg
     8401 gaatgaatct gacccgtcgc accttactgg gggtaatgaa atatccggca ctcttgtctc
     8461 aaacctcgcc gattgagccg gcagaaatga gcgaggctcg gcatattaat ttgcaccatt
     8521 ttccacagag caaagggatt tttgatgaca acaaccactt ttttgagtgg gtattagccc
     8581 cgttaagtga aaaagaccga cgccaatttt gcacggttca gcccaatcaa gatccgaaac
     8641 agccaaacaa aacgcaatat aaatctctcg attgcagcat aatggaacta gcggacgata
     8701 ttgcctatgg cgtacacgat ttggaagatg cgattgtagg tggaatggtc acaccgcaat
     8761 cgtggcaaaa tgcggaaaaa ttgctggcgg agtgccaatc cgattgggtg aaacaacgcc
     8821 tgccggagat tcgggaaaag ctcttttctc agcaccgcta cgagcgaaaa gatgtgatcg
     8881 gggcattagt gaatcatttt attaccaatg tgcgttggaa agctctgcct gaatttgacg
     8941 agccgttgct acgttataat gcctatttac cggaaagtgt tgcctgtgtg ctgaaaatct
     9001 tgaaagattt tgtgtatcaa tatgtgattt gtgatgccaa aacccagcgg gtggaacgca
     9061 aggggcaacg cattctaatg gaattgttcg atattttaag cagtgatccg atgcgattac
     9121 tccctaataa tgtgcgggaa cgttggaaaa atgcggaaga atctaagcgt gcaagggtaa
     9181 tttgcgacta tttagccggt atgtctgatg ggcaggcgtt taaacttttt gaaagtttat
     9241 aatgaacttt gttaattttg caaggtctca gtaataggtt tcatttattt aataggagat
     9301 ttcaatgaaa actgtttcta aaggtctttt agtattagca actttggctt ctgcaacagc
     9361 atttgcggtt tcttccaagt gaaatttatc agcctcgtgg cgaattagtt aaagcagatc
     9421 gtcaaggtaa tggtgagttt gaagtagaat atcgtgtcag tggtaatgat gttcgtggct
     9481 tagcgaaaag tgcgatcgcc cacgctaaac gtaaaggttt ccatttagta gaatctgata
     9541 ttcatagaga tgatgctgat ttaaaattca aacgtggtga ccaagagctg gatattgaaa
     9601 ttgaagtaaa aggtagaaat cggattgaat ataaggcaga tctagattta gataaaaact
     9661 aaatttaccc cttaaaacga ccgcttgtgt gatttaaccg cacaagcggt ctttttattg
     9721 tgcttttttg caaaaatact caagccgacc ggtcccggaa tttggtaaaa tagccgttta
     9781 aatgacaata atgatgcttt tacttattac ttagaggaaa aaatgttttt agtcggtcag
     9841 cgttggctaa gtgaatcgga aaataattta ggtttgggaa tcgtgacttc ggtggatcat
     9901 cgcactgtca ctatcaattt cccggcagcg gaagaagagc gggtctatgc aattgcagtt
     9961 gcacctttaa ctcgagtgca gtttcaagcc ggagatagaa ttaacagtat agatggctgg
    10021 cagttagagg tttcagaggt tgtggaaaat caaggtgccg taatctattt gggcaaaagg
    10081 gtggataacg gtgaagaagc ggtactgcct gaaatgttgc tcgatcataa aatcagtttc
    10141 agcaaaccgc aagaccgcct tttttcggct cagattgatc gctcagatcg ctttgcatta
    10201 cgctttaacg ctttgcagca ccagcaagcg caatatcagt cgccactgcg tggtttgcga
    10261 ggcattcgag ccagcctgat tccacatcaa ttacatattg caaaagaagt cgggcaacgc
    10321 cttgctcctc gtgtgctatt agccgatgaa gtgggactgg gtaaaactat tgaagctggt
    10381 atgattttgc aacaacagct attttcgggc agagcagaac gagtattggt attagtgccg
    10441 gaaagtttgc aacaccaatg gttggtggaa atgcttcgcc gttttaatct gaaattctcg
    10501 ttattcgatg aagaacgttg tagtgatttt gataaacaag atgaaaatgg cgatgatgta
    10561 agtgaaaatc catttgagag cgaagcgtta gtcattactt cactaaactg gctggaaacc
    10621 tcaccgcaac gagccaatca agtgcttgct gccgactggg atctattaat tgttgatgaa
    10681 gctcaccatt tagaatggtc ggaaaccgaa tcttctatcg gctatcagtt ggtagagcgt
    10741 ttatctcaac aaattcctgc cgtcttgctt ttaacggcaa caccggagca attagggcaa
    10801 gagagccatt ttgcccgctt ggcgttgtta gattcggatc gtttttatga ttatgccgag
    10861 tttgtacagg agcaagcaca gtataaaccg gtggcagatg cggtgagtag cttattaagc
    10921 gataaaccgc taagtaatga cgagcagaat gcgattgcgg agctgttaag cgagcaagat
    10981 accgagccga tgttccgtgc aattaatagc aaaaaatcgg acgaaaatga ccgcttgcaa
    11041 gcccgtcaag agctgattag tgagctaatt gaccgccatg gaaccagccg ggtgttgttt
    11101 agaaataccc gtcagggagt gaaaggcttc ccacatcggg aataccacca aatcagccta
    11161 gaaatgccga aacaatacca aaatgcgtta aaagtgatgg gaatgatggg gagtgtgagc
    11221 caaagtgatt tgctctatcc tgaatatttg ttccaacgaa tgaacccgaa tgcgaaatgg
    11281 gctgaattcg accctcggat tgattggctc atcactttct taaaaaatca tcgtgatgaa
    11341 aaagtgctgg tgatctgcaa acaggcggac actgcgattg ctttggagca agttttgcgt
    11401 gagcgtgagg cgattcgttc tgccgttttc cacgagaaaa tgtcgattgt tgaacgtgac
    11461 aaagctgccg cctattttgc tcaacaagaa gaaggcgctc aggtgctgat tagttcaagc
    11521 atcggttcag aagggcgaaa tttccaattt gctaaagatt tagtgctgtt taatttgccg
    11581 gataacccgg atttattgga gcaaagtatt gggcgattag atcgtatcgg acagaaaaat
    11641 gacattcaga tttatgtgcc ttgctttaat ttatcggctc aacagatgtt ggcagagtgg
    11701 tatcacaagg gcttgaatgc gtttgaagaa acctgcccga tgggaacagc gatcttccgt
    11761 gaattaggtg aagaattgca acaattcctg caaaaacctg aactttcagc ccaatttgat
    11821 gattttgttg cgaaaaccta taaacgccaa cagtatttga aagccgaact ggagaaagga
    11881 caggacagat tattagaact gaattctaat ggcggagaag cagctcaaca gctcgctaat
    11941 gatattgcga aagaagataa taatccgcag ttggttaatt ttgccttgag tttgtttgat
    12001 gtgattggcg ttgaacagga agatttaggc gagcagagca ttgtgattag cccaaccgga
    12061 catatgcttg tgcctgattt cccggggcta tcggaagatg gcacgaccat tacctttaat
    12121 cgtgaactgg cgttaatgcg ggaagaggtg gaatttctca cttgggatca cccgatgatc
    12181 cgcaatggta ttgatttaat cacctccggc gatattggca aatcagcaat ctcattgctg
    12241 gtgaataata aattgcctgc cggtacatta ttacttgagg cgctttatgt ggtggaagca
    12301 caagcaccga aagggctaaa tttaacccgt ttcctaccgc caaccccgat tcgggtttta
    12361 gtggataaca aaggcaacaa tattgctccg caggtctctt ttgccgggct tgaacgccaa
    12421 ttaaaaccgg tgaataaaca gatggcaaat aaaattgcca aaatggctcg agcggatatt
    12481 gaaaaattaa ttaaagcgag tgaaaaagcg gtagaacctc aggtccaaca ggtgattgaa
    12541 actgccaaat ttgaggcaaa tagcaaatta aaagcagaat tacaccgctt ggaatcactc
    12601 aaaatggtga ataaaaatat tcgagccgat gaagttgaag ccttagaaca acagcttaac
    12661 gcttcccttg cccaacttga aacggcaaat tatcgcttag atagcttgcg agtgattgta
    12721 agtaataaag cgtaattttt tgcaagtacg aaaagtgatt ttttcgatct gtgtcgaaaa
    12781 aatcactttt ctattttgtg tcatccttgc agattattta tgctattcct catattgtaa
    12841 aaggtataaa aaaggaatat tatgtatata gatttgcaat atgctgtgcc gttattgttt
    12901 gaatacgacc cgaacaaaag ccaaattaat ttagccaaat atggtattga ttttgaacag
    12961 gcaaagttgc tttgggaaga tgaacgaaaa gttgtactgg aggcttgcat tgaacccgaa
    13021 ttacgctatt tcctgattgg caagatttat gataaatatt ggacagcgtt ttttacacca
    13081 agaaatgatg ttattcgttt aatttcagtt agacgttccc gtaaaaaaga gatctcatac
    13141 tatgagaaat accattagtg ccgaagaatt agaacgtatc ttcgatgaag gcaaagaaga
    13201 tattttacaa tactgcgatc tttctaccgc tcatcgcccg gggcaaatga aaagggtgaa
    13261 tgttgatttc ccaaattgga tggtagaagc gttagataag caagccagac atttaggtgt
    13321 gcctcgccaa gccttgatta aaatgtggat tgcggaaaaa ttaccggttc aatcacctag
    13381 gtaagcgagt ggcaaatggc attaattgaa tataaccctc cattagaacc atttttagat
    13441 gaggtttatc gggataacca tattttagta attaataagc ctagtggttt attatcggtg
    13501 ccgggcaatc gtcccgaata ttacgacagt gcaatgactc gagtacagca aaaatatggc
    13561 tttaccgaac cggctcatcg tttggatatg gcaacaagcg gtattctttt gttcgcccta
    13621 agcaaagcgg cggaaaaaga actaaaacgc caatttcggg aacgtgaacc gaaaaaacac
    13681 tatattgcgt tactttgggg caaattaggt gaaaaagttg gggataaggg cgaaatgaat
    13741 tttccgctga tttgcgattg ggaaaatcgc ccccgccaga aaatttgcta tgaacgaggc
    13801 aaaaaagcca ttacccatta tgaagtgtta gctcattatc cgaataatat agcgagagta
    13861 aaactcacgc ctatcacggg gcgttcgcat cagctacgag tacatagcct tgcgttaggt
    13921 cacccgatta ttggcgataa gttttatgct aatccgctgg caaaaagttt atcgccccgt
    13981 ttatgtttgc acgcggagtc actaacaatt actcacccga ttacaaggga gttgatgacg
    14041 tttactgctg agccgacttt ttagcctcgt tcagtaggtg tttgtaatat gcccaatcaa
    14101 aatattgccc cgggtcgatt ttccgttccg gggcaatatg gcaatgcccg gtgatgcgtt
    14161 catcggtaat ttgtggatag ctttgctgaa tggctttggt caaatttgcc aaagattggt
    14221 attgagcttg agtaaacggc tgattattac tgccttccag ttcaatgcca atcgaatatt
    14281 cattgcattt ctcccgccct tcaaaacaag agacgccggc gtgccacgcc atatcgttaa
    14341 aattcacata ttgcgtgatt tccccctccc tattaatcaa acaatgggcg gaaacccgta
    14401 gatctttgat ttcttcaaaa taagggtgag cggttggttc gagtttacct tggaaaaaca
    14461 gatcgataaa atttccacca aattcttccg gcggtaagct gatgtagtga ataaccaaca
    14521 aggaaatatc ctcttgatca ggacgttggc taaaatgcgg agacggcact tgtttttgac
    14581 cggttaaaca gccctgttcg atttttaaat ttggcatagt ctaaataggg tgaatacgtt
    14641 aaaatatggc tcattttact agaaaaagag aagaatatgt taagttatcg ccacagtttc
    14701 cacgcaggca accacgctga tgtgctaaaa cacattgtcc agcttttgat cttgcgagca
    14761 ttaaaagaaa aagaaaaagg ttttttatat cttgatactc actccggtgt ggggcgttat
    14821 agtctgttaa gttttgaatc ggaaaaaaca ggcgaatatt tagagggcgt tgcccgttta
    14881 tgggagcgtg atgatttacc ggaagaagtg gcattctata ttgatgagct gaaaaaatta
    14941 aaccgtggcg gcaagttaaa gtattacgca ggctccccat tattggcggt acagcaactg
    15001 cgtgagcaag accgagccat tcttaccgaa cttcacccca atgattttcc gctgttacga
    15061 caagaatttg ccaaagcgaa aaatgtgcaa accaagcgag aaaatggctt tcaacaatta
    15121 aaatccgccc tgccgcctaa agagcgtcga ggttttgtgt tgattgatcc accttatgaa
    15181 ttaaaagaag attatgaatt ggtggttaaa gcgatagaag agggctacaa acggtttgcc
    15241 actggtgtgt atgccatttg gtatccggtg gtgcttcgcc aacataccaa acgtatagtg
    15301 cgaggtttaa tggataccgg cattcgcaaa attctacaaa ttgagcttgc cgttcgtcct
    15361 gatagcgacc aacgtggaat gacggcaagt ggtatgattg tgatcaaccc accgtggcag
    15421 cttgaaaccc agatgaaaaa aatcctgcca tatttaaccg aggtgcttgt gccggaagga
    15481 acaggttctt ggaaggtgga gtggattacg cctgagtaaa ttaaatacgg atagcacaag
    15541 cgtggacgct tgcgccatca taaattctga tttgcaatat tttttagaaa aataaccgct
    15601 tgtcttatca agaagtgggg gaaatataaa aggaaaaaac gatgcaacaa tacaaacacg
    15661 actttattga atttgcctta agccgcaatg tgctgaaatt tggcgaattt accttaaaat
    15721 caggcagaat cagcccctat tttttcaatg ctgggctttt taacaccggg cgcgatttag
    15781 ccaaattagg cgaatattac gccaaagcca ttcaagccag cggcattgag tatgatgtgt
    15841 tattcgggcc ggcttataaa ggcattccga ttgcaaccac ggttgccgtg gcactagcca
    15901 acctgttcga tgtggataaa ccttgttgct ttaaccgcaa agaggcaaaa gatcacggtg
    15961 aaggcggtaa tttaatcggt tctcccttaa cgggcaagat tttgctggtg gacgatgtga
    16021 ttaccgcagg cacggcaatt cgtgagtcga tggaaattat caatgccaat aatgcccagc
    16081 ttgccggtgt gctgattgcg ttaaaccgta aagagaaagg caagggcgag ttgtctgcga
    16141 ttcaagaagt cgaacgtgat tacggttgcc aagttttctc gattgtggat tttgatgatt
    16201 tggtagcatt tattgaaacg tcgccacaat acgctcctta tttgccgaaa atgcgtgaat
    16261 atcgtgaaaa atacggtgtt taacttttgc aaaaaaacag caaaaactca ccgcttgtca
    16321 ctctctaatc tcaccattat gaatcacttt catcttttgg tgagattttt tcaattaaaa
    16381 agccgataaa ttgattgtga gctttagcta attggcttag gataaactcc tgtaatacag
    16441 gagttggaga actaaaatgg cacatcaaaa attacctttt cacgcagaca ccgttggctc
    16501 atatttgcgt tccgatgctt ggaaaaaagc tcacgcagac tataaagcag gcaacatttc
    16561 cctcgaacaa cgtgatgaaa ttgtagaagc ggaagtgaaa aaattagtac aagcatcaat
    16621 tagatgcggg cattcaagtt gttacagacg gcgaatatca ccgctcttgg tggcatattg
    16681 atttcttgga aaatctgaac ggcattgaag gctatgtgcc ggaaaaagcc tatgcgttta
    16741 aaggggttga ggttcgccct tataatacac gttgttgcgg taaagtgtct tggaatccga
    16801 atcacccttt tattgagcat ttcaaaaaat tcaatgcgat tgtaggtggt cgtgcggtgg
    16861 cgaaatttac cattccaagt ccaaaccaat taatgtaccc gattcagtgg gatcacggca
    16921 tttacccaaa ccgtgaggaa tttgccaaag atgtgcaaca ggcttataaa gaggcgatta
    16981 aagcgttcta tgatgccggc tgtcgttatt tgcaaattga tgatgtctat tgggggtcgt
    17041 tgtgtaataa cgttggtcaa ccgacatttg aagccgataa agctcaagcg gtggcgaata
    17101 ttcaagccat tcttgccgat aagccggaag atttaaccat taccacccac gtttgccgag
    17161 gcaactataa atcttcttat ttattgacag gggcttatga tccgattgcc gacggcttat
    17221 ttgctcaaac caactatgat gggtatttct tagaatatga tgacgagcgt tcaggcggtt
    17281 ttgagccgtt aaaacatttt gccaacaaca aagggcgtgt ggtattgggc ttaattagtt
    17341 caaaattccc tgaattggaa gataaggaag cggttaaagc ccgtattcaa gaagcggcaa
    17401 aatatgtgcc gttagagcag ctttgcctaa gcccgcaatg tggttttgcc tcaaccgaag
    17461 agggcaatat tatgtcggaa gcacagcaat gggcgaaagt tcgtcttgtg gaggaaatcg
    17521 cccaagaagt ttggggcgaa gattaattag agtgaaaaat gcccgtaaca agcgggcatt
    17581 ttttgtgaat tttttgcaaa ttcaagccat ttagcgaatg cggttaaaac cttgttctaa
    17641 atcggcaatc aaatcttcca ccgcttccaa gccaacgtga atgcgaatta atgttcccgt
    17701 cagtttgcgt tcaatcgccg ggcggatttt ggcaatttct tccggctgat tggcaagaat
    17761 cagcgattca aacccacccc aagaatacgc cattgaaaac agttcaaaat gctctaggaa
    17821 gttggctaat ttgtcattcg agagcttttc gcaaagttca aatgaaaata gaccgcttga
    17881 acctaaaaaa tcccgcttaa aaaattcgtg ccccgggcag ctttccaacg ccgggtgata
    17941 aactgctttc acttcagggc gttgagccag ccagttagct accgcaatgc tactttgttc
    18001 gtgctgtttt aagcgaatgc ctaaggtgcg tagcccccga gcggtgatgt aagcggaatc
    18061 agcatccacc atttgcccca ttaaataaga gccttcacgc agtttatccc aacaacgggc
    18121 gttagaaacc gcagtaccga tcatcacatc agaatgcccg actaaatatt tagtgccggc
    18181 ttgaatggaa atatcaatgc catattctaa agcagggaac agcacaccgg cagaccaagt
    18241 gttgtcgatc ataatcacga tttctgggtt gatttcacga gcaacacgca cgagagtcgg
    18301 aatatcaggc acttccattg ttagcgagct cggcgattct aaaaaaagca ctttggtgtt
    18361 cggttgcaca agggctttca agccttcacc aatcatcggg tcataataag tagttgaaac
    18421 acccatacgt tttaacagcg cattacagaa ttcttgtgtc ggttcgtaag ctgcgcctgt
    18481 cattaaaacg tggttgcctt gttccacaaa agctaaaatc gcattggtga ctgccgccgc
    18541 accgcaaggg taaaggtagc aacctgctcc accttctaat tcacacatcg cttcttgtaa
    18601 tgaaaaatga gtgagagtgc cacgtctgcc ataaaataat gctcctttgt agcggttttg
    18661 tgtagcgtgt tttttctctt ctaaggtttc aaacactaaa gatgaagcac gctgaatcac
    18721 cgggttaact gctccttgtg tgttacgttt tttacgccct gtatgaacaa gtttggtgga
    18781 aagattttta tagttagaca taacttttcc taaagtaaaa tgcatagggg caaatcaaca
    18841 tttgcccctt tattaataat taacgaaaca taaagttatt tctttgacgg ctacgtttaa
    18901 attggctgat tagcacaatg accgcaacaa ttaagtaaac cgcaaaagta aagattagcc
    18961 attgtgccat tgaaaagccg aacatttccc attggctttg gctacaatta accggaccgg
    19021 gggcaaacac ttgtggtagc cattggtcaa gcggcaaggt ttgcgggaaa ttaactttaa
    19081 attcgcattg attccatggt gatggattga cctgataatc gtggtgtttg attgaaagta
    19141 ctagcccttt aaatgcgcta ccaagccaaa ttagaattgc caagtagcga ataatcgcat
    19201 atctattacc aagaaatcct aaaataccgg caaataaaat accaaataaa gcaactcgct
    19261 cataaataca cattacacaa ggtactaatc ccatcccatg ttggaaataa agcgctgtgc
    19321 cttcaagtat aagacagctt aacattagta aaaaccatgc actgcgagat tgtgatagat
    19381 ttttaaaaaa atagagcata gttattcctc attttgtgaa cttaaggcat tctaccttat
    19441 ttggtatgag ttatctataa actaacgtgt aataaaccat ccccaattta tcattacttc
    19501 agtcatagaa ggtagtaaga attctaaccc taagaaaccg acaatagata gaacaatggt
    19561 ataaggtaat gccatataga ccattctgcc gtaagacagt ttaattaacg gcgcaaagga
    19621 ggacgttaat aagaataaga aagcagcttg tccgtttggg gtagcaacag aaggaagatt
    19681 tgtgccagta ttaattgcaa ctgcaatcag gtcgaactgc tcacgatcta taatgccggc
    19741 atctagtgcg gttttagctt cattaatgta aacagtaccg acgaagacgt tatctgaaat
    19801 catcgagagc aaaccattga aaatatagaa taatgaaagc tgtgaatgtg gttcggcaga
    19861 taacacaaag gcaatgatcg gctcaaataa ttttaaatca ataatcacgg caacaaccga
    19921 gaaaaacaca ataagcaatg cagtaaaagg catagattct tggaatgctt tccctaaagt
    19981 gtgttcatca gtaatgccac aaaatgcagt gcaaataatg atgatggtta agccaataat
    20041 ccctacatcg gcaagatgta gcgctaagcc aataattaac caaattccag caatggcttg
    20101 caccagcatc tttaagcgat cttgtttcgt cattttttgt tcttttagtt ggctatagcg
    20161 agcgagtacg acccaaactt tgctaggaag ccggtctcca taaccaaata atttatattt
    20221 ttctacaaga aagcaggtcg caatcccaca aattaacacg ggtaagctaa ctggtaatgc
    20281 tctaagtaag aattcgataa atccccattc cgcttgggag gcaataatta agttttgtgg
    20341 ttctcccacc atggtcatta cgccacctaa agctgagcca ataccggcgt gcatcaataa
    20401 acttcttaaa aaaccacgaa attgctctaa aatttcttta ttagagatga ttttttcatc
    20461 attggtaata tcagtagaat cttcaaagtt attacctgat gcgactttat gatatacgcc
    20521 gtagaaacct gttccaacgc taatgattac agcaataacc gttaaagcat caagaaaggc
    20581 ggataaaaaa gcagcgctta agcagaaaga aagggaaaga atttttttcg aatgaatggc
    20641 gacaagcagt ttggtgaaaa tataaagcaa taattgcttc ataaagtaaa taccggcaac
    20701 cataaacatt aaaagaagaa taacatcaaa gtttgccata atttcttctt taatgtggtg
    20761 aggatttgtc ataccgatta caactgcttc aatcgctaat aaaccacctg gctgtaatgg
    20821 ataacatttt aatgccatag agagagtaaa aataaattca gcgactagaa accagcctgc
    20881 aacaaaagga ttgataaaga aataaatgag aggattgata attaaaaatg ccaaaatgca
    20941 caatttatac cactcggggc tttttcctaa gaaacttttg aacagagcat caatgctact
    21001 catataaagg acctcaaaat ttgtaaacag atattacaat tgtaatatag tttagattaa
    21061 aggataatag tattactgaa ctttttagtc gcaataattg attagaaata aagaatagtt
    21121 agattttaga taagcatatt ttttgcttgg ggggcattaa tgggtcacag tgatatttta
    21181 aaagcgcaaa gcccggcagc gttagcggaa gaatatattg taaagagtat ttggaataat
    21241 catttccctg ccggctctga tcttcctgcc gagcgagagt tagcagagcg cattggggta
    21301 acaagaacaa ctttacgtga agtactacaa cgtctagcgc gagaaggttg gttaagtatt
    21361 cagcacggca agccaacaag agtgaatgat atttgggaaa cttcggggcc taatattatt
    21421 ggcactatta ttaagctaga taaaagctat cttccagtgg tgattgccaa tgtggtgtca
    21481 ttacgtactc gtatggcgga ggcttatatt ccagaagcga tttaccttgc tcctgaagaa
    21541 agtgctaaac tctttgatgg actagatgaa ttagaaaata cagcagctgc ttatgctgaa
    21601 tttgattata agttattcag acaatttact tttttagcca ataagcctgt gtatgcgttg
    21661 atcctaaata gctttaaatc aatgtatcat caagtcgcaa aaattttctt tgctgatgaa
    21721 aataatcgtc aattaacgtt aagcttttac cgttcattat tatgtacttg tcttgcaaaa
    21781 gatagtgaaa aagcagccgc ttgtatggct aaaaatcgcc aatctagtgg tgaaatttgg
    21841 caaaaattat ccgctaatat tcctgaaagc tttggtcagc tttaacagaa ggtagaattt
    21901 tgaacgaaat tatttttagc ttagaaccgc aagataatgc tcgtctgcaa tcgctttgtg
    21961 gtgcatttga tgagcattta gccttaattg aaaaaagttt taatctcatt attgctcgaa
    22021 caggcttcaa ctttacgatt caatcggaag atgacaatca ctattctgct accttggttc
    22081 aaaatagtgt gaagctgatt aagcaactgt ataatgaaac tgccccgatt aaaggcaaaa
    22141 ttaaggaatt ggacttagaa gatatacatt tggcgattca ggaaagccga atgctactac
    22201 aaaaccaatg gcaagccggc tcgcaaggcg aaatagcgat taaaaccaag cgaggggtga
    22261 ttaaacctcg tagcgaacat caacaagcct atttacgaaa tatattaagc cacgacatca
    22321 gttttggtat cgggcctgct ggcacaggca aaaccttttt agcggtagcg gcagcggtgg
    22381 aatcgcttga acgccaagaa atccgccgta ttttacttac ccgtccggcg gtggaagccg
    22441 gtgaaaagct cggtttttta cccggcgatt tagggcaaaa aattgagcct tacttacgcc
    22501 cgttatatga tgccctgttt gaaatgcttg gctttgaaaa agctcaaaag ttgatggaac
    22561 gtggtgtgat tgaaattgcc ccacttgctt atatgcgagg gcgtacttta aacgatgcgt
    22621 tcatcatttt agatgaaagc caaaacacga ctaccgagca aatgaaaatg ttcttaaccc
    22681 gtattggctt taattccaaa gcggtgatta ccggcgatat tacccaagtg gatttgcccc
    22741 gtagccaaaa atcaggctta aaacacgcaa tggaagtgct aaaagaagtg ccggaactca
    22801 gctttaattt ctttgacagc aaagatattg tccgccaccc tgtggttgcc aaaattgtgc
    22861 aagcctatga tgcgtgggaa gcggttgatg aagagcgtaa agaacagcga aaactggaaa
    22921 aattagcttt agagcaagaa aaaattcttg ctcaagctga aatgttatag cttacaagcg
    22981 gtcatatttc gctaattttt tgcaaaaaat cgctgaaatt tcaccgctta tcataacagt
    23041 tgttcaacaa aaaagcggga agttaattcc cgcttttctt tatcttattg tttcattata
    23101 agatttcttt tgctttagca accacgtttt caacggtaaa gccgaagagt ttgaataatt
    23161 gatctgctgg tgcggattca ccaaagctat tcatacctac aattcgacca tcaaagccga
    23221 catatttata ccagaagtca gctaattgag cttcaatcgc cacacgttta gtgactgaac
    23281 gtggtaatac cgattcacga taggcttcat cttgtttgtc aaacacgttg gtgcttggca
    23341 ttgaaactac acgcactttc acgccttctg catctaacac atcagcggct ttcattgcta
    23401 aatctacttc agaaccggtt gcaattaaga ttaaatccgg gcaactgcct ttttcgcaac
    23461 attcacgcaa gatatagcca ccacgagaga tgttcgctaa ttgctccgca gtacgttgtt
    23521 gttgagccag attttggcgg gtaaagatta gtgctgtcgg gccgtcttta cgttctaccg
    23581 ccgctttcca agcaacagca gattctactt ggtctgccgg acgccaggtt tctaagtttg
    23641 gaatttggcg taatgaagca gtttgttcta ccggttggtg ggtcgggccg tcttcaccta
    23701 aaccgatgga gtcgtgtgta taaacaaata aggcacgttg cttcattaag gctgccatac
    23761 gcaccgcatt gtgtgcatat tcgtagaaca ttaagaaggt tgcaccgtaa ggaatgaaac
    23821 caccgtgtag ggcaatcccg ttcataatcg cagacatacc gaattcacgc acgccgtagt
    23881 tgatgtagtt accatctaca ttgtgttccg cacgaatcgg ttttgagcca gaccataagg
    23941 ttaagttaga gcttgctaag tcagctgagc cgccgaggaa ttcaggtaag attggcgcgt
    24001 aagcttcgat tgcattttgt gatgctttac ggcttgcaat ggttgccgga tttgcttgta
    24061 atttttcaat aaaagctttg ctttcagctt cccagttagc cggtaattcg cctgcgtaac
    24121 ggcgtttaaa ttcagcggct agttcagggt atgccgcttc ataagcagca aatttagcgt
    24181 tccaatcttt ttctgctaat gtaccttttg cttttgcatc ccactctgta taaatttcag
    24241 ctggaatcac aaatggggca tgttcccaac ctaaaaattc acgagcggct tggatttcag
    24301 catcacccaa tggcgcaccg tggcaatcgt gagagtttga tttatttggt gaaccatagc
    24361 cgataatggt tttacaaata attaaagttg gacgctcggt ttcggcttgt gcattttcaa
    24421 tcgcaaattt gatttgttct gggttatggc catcgacatt acggataact tgccagccat
    24481 aagactcaaa gcgttgtgcg gtatcatcag tgaaccaacc atcaacgtgg ccgtcgatag
    24541 agatattgtt atcatcgtag aaagcaatca gtttgcctaa gcctaatgta ccggcgagag
    24601 agcaagcttc gtgtgagata ccttccatta aacagccatc gcctaagaaa gcgtaagtgt
    24661 agtgatcgac aattttatga ccttcacggt taaattgtgc tgcaagggtt ttttccgcaa
    24721 tcgctatacc gaccgcattg gtaatacctt gacctaacgg gcctgtggtg gtttcaacac
    24781 ccggtgcata accatattca gggtgaccag gtgttttaga atgtaactga cggaactgtt
    24841 ttaaatcttc aatagagaga tcatagccgg ttaaatgaag taagctatag attaacattg
    24901 aaccgtgtcc gttagacaga acaaaacggt cacgatctgc ccatttagga ttagttggat
    24961 tgtgttttaa aaaatcacgc cataaaactt ctgcaatatc agccataccc attggtgcgc
    25021 ctgggtggcc tgagttagct ttttgtacag catccatact taaaaaacga attgcattcg
    25081 ctagcacttt acgttctgtc atttgtgtta ctcctaatcg agaatgttat ataaaatgga
    25141 aaggatatcg aattggattc tacactattt tgctagaaaa actcaaaaat ggaaatgaga
    25201 aatgtgatct gtatcacaaa aaatgattaa attgtgattt gttttgattg gatttgattt
    25261 taatagacgg ttttttccta atacaatatt ttcttgataa agcgaatgtt tagtagttga
    25321 tctaggcaac aaatttatct aaaatacgca ctctttttgg cgttgagcat ttgctcaaca
    25381 tttttattaa ttacatatgg tacctatcag cggtagagat tctaacaaga taggcggcga
    25441 tatggcttct tatgaggttc cccaatgaaa caaggtattc acccagaata taaagaaatt
    25501 acggcaactt gctcttgcgg taatgtagtt aaaactcgct caacagtggg taaagattta
    25561 aacttagacg tatgtggcaa ctgccacccg ttctatactg gtaaacaacg tgttgttgac
    25621 accggtggtc gtgttgaacg ctttaacaaa cgtttcagca tcccaagctc aaaataattt
    25681 gattattgca aaccccgcta atgcggggtt ttgtttttct tatatatcga attaacaagc
    25741 ggtgtatttt tctatcaatt ttgcaaattg gtgatttaac cattttgcag gagctaccag
    25801 agaaaaacct cgagtatcat tattatcctc ggtccattta tctgcgatca ccataaaagt
    25861 ttcaacatct ttcttaggtt ttggatacca gcgtgcaata ttagctggat gattggcata
    25921 aaatgagagc aatttcttgc ctaatgcaca agaaatatgg acaccacctc catcaactgc
    25981 aattacaata tcagcagagt ttactaatgc acaataacct aaaatatcac tattaggtgc
    26041 taggcttatt tcaaatgggg ctttaatttt cgacaaatat tcggcactat tggttgtatt
    26101 ggtcagcaaa caactaatat gttgaacata tcttttatca atcatagata atagctctga
    26161 taattcttga gtagaaattt cacgcatact accttgagga ttcaatagta atcgaatttt
    26221 cttctcttgc catagcgtat ttgcaaaact gcttagttca ttaggaatgt ttaaattata
    26281 attgacctga tttttttcta aaaaatcagc aaaaatagca tagttgagat agtcagcaaa
    26341 atgaacctgc tttggaaaga cagtggtgaa atcgtaattt tttactgtat ctaaattgta
    26401 aatacttt
//
LOCUS       DANLZC010000016        25194 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.016, whole genome shotgun sequence.
ACCESSION   DANLZC010000016 DANLZC010000000
VERSION     DANLZC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.016"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            51..2350
                     /locus_tag="PXE44_000530"
                     /pseudo
     CDS             51..2350
                     /locus_tag="PXE44_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759321.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(2428..3129)
                     /locus_tag="PXE44_000531"
     CDS             complement(2428..3129)
                     /locus_tag="PXE44_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDL5588072.1"
                     /translation="MDLQNRENFLNKLAQRMGRPRQNVPEPIPVLENNHAITRLTDRP
                     QPQLCQEFIDFARVMMADVVEAKEADLPQAIIDICEKYGGASVIVNNDQRLKDLGITA
                     AIQAKYEDSYVWDFNQAETNFEKARTANIGVVYGEYGLAESGGIVLFSDKEFGRSVSL
                     LPEKSVVVLRKSTVLPRVAQLAQILHEKAQRGERMPSCVNIISGPSATADIELIKVVG
                     VHGPVNKIYLVIDDL"
     gene            complement(3134..4543)
                     /locus_tag="PXE44_000532"
     CDS             complement(3134..4543)
                     /locus_tag="PXE44_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDL5588073.1"
                     /translation="MSYLQTNTLPFKQRVEQQVNNEIVRKALVKAQETIGANRQRMVD
                     ELGNWEEWRDDAKAIRNHVLANLDAYLYQLSEKVTQNGGHVFFAETAEEATDYIKNVA
                     KAKNAKKIVKSKSMVTEEIGMNHVLEAEGIKVVETDLGEYLLQIVGDKPSHIVVPAIH
                     KDRHRIRQEMHDVLGYQGSETPEEMTAFVRQKIREDFLEADIGISGCNFAVPETGSVC
                     LVTNEGNLRMATTVPKTHIAVMGMERIAPTFKEVDVLITMLARSAVGAKLTAYNTWLT
                     GPRLEGETDGPEEFHLVIVDNGRSKILESEFQEVLRCIRCGACLNTCPAYRQIGGHGY
                     GSIYPGPIGSVISPLLGGYEEFKELPYACSLCTACNSVCPVKIPLAQLILKHREKIAD
                     AGLTPMAERLSIFGFNFANSHPTLWKVGVKVGAKVASKLIKNGKAPVNFGALGEWTKA
                     RDLPTAEGESFREWFNNRG"
     gene            complement(4545..5276)
                     /locus_tag="PXE44_000533"
     CDS             complement(4545..5276)
                     /locus_tag="PXE44_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDL5588074.1"
                     /translation="MNVNFYVTCIADVMKAGVAKNTVLLLEKLGCKITFLEKQGCCGQ
                     PAINSGYTKQALAGMKNLVETFEANDYPIVAPAGSCVYAIKTYPDHFERFGEKEWAER
                     AKKVADRFYDLTDFIVNKLGVKDVGAYLPGKAVYHPSCSLFRKLGIKDEPIALLQHVK
                     GLELLPIKNQETCCGFGGTFSVKMAEISGEMVKEKVANVDTDEPDYLIGADVSCLMNI
                     GGRLSREGKPIKVMHIAEVLMQGGK"
     gene            complement(5420..7024)
                     /locus_tag="PXE44_000534"
     CDS             complement(5420..7024)
                     /locus_tag="PXE44_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL5588075.1"
                     /translation="MALFLSVFPIVLLIYLMVKRNALPSYVALPLTALLIFVLQLTYF
                     GNDTTLIFANIIAGLGDVLTPITVIFGAILFNRFSEVSGATNTMRKWLGTINPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILVGLGFKPLQVAMLALVMNSVPVSFGAVGT
                     PTWFGMGPLLKDGLLTDAQVLEIGSITALIHSIAAFIIPVMALRLIVSWKEIRQNIVF
                     IYISIFACVIPYFIIAQFNYEFPSLVAGAIGLLVSVWVANMGIGLAKSENHLDGDKAT
                     FGEVAKALLPTGLLIFILVITRIQQLPLKAMLNDATAWIVSSLGFANFEISQGLIFAL
                     KNILGTNVATSYKLLYVPALIPFVVTVLICLPIFSVSGKNTQEIFGTSFKQVKKPFLA
                     LLGAIVMVKLMLIGGVDSMVQIIGKNFAEFTGQSWTLFASFLGAIGAFFSGSNTVSNL
                     TFGTVQYATAQQVGLSVTLILALQSVGGAMGNMVCINNIVAVCSVTGIEKQEGNIIKK
                     TAVPMFVYGVIAAISALFIVPMFYNL"
     gene            complement(7314..8405)
                     /gene="ychF"
                     /locus_tag="PXE44_000535"
     CDS             complement(7314..8405)
                     /gene="ychF"
                     /locus_tag="PXE44_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL5588076.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNTGVV
                     PMPDPRLDALAEIVKPERVLPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGH
                     VVRCFENDDIVHVAGKIDPADDIEIINTELALADLDSCERAIQRLQKRAKGGDKDAKF
                     ELSIMEKILPVLENAGMIRSVDLDKDELHAIKGYNFLTLKPTMYIANVNEDGFENNPY
                     LDRVREIAEKEGAVVVPVCAAIESEIAELDDEEKVEFLQDLGIEEPGLNRVIRAGYRL
                     LNLQTYFTAGVKEVRAWTVSVGATAPKAAAVIHTDFEKGFIRAEVIAYQDFIDNKGEA
                     GAKEAGKWRLEGKDYIVQDGDVMHFRFNV"
     gene            complement(8591..9178)
                     /gene="pth"
                     /locus_tag="PXE44_000536"
     CDS             complement(8591..9178)
                     /gene="pth"
                     /locus_tag="PXE44_000536"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL5588077.1"
                     /translation="MSEIKLIVGLANPGVKYEETRHNAGEWLINELARIYNVSLKEEP
                     KYFGKVAKINTAQGEVRLLVPTTFMNLSGKAVGALANFYRIKPEEILVAHDELDLPPG
                     SVKLKKGGGHGGHNGLKDIIAALGNNKEFYRVRIGIGHPGHKDLVANYVLGKPSPSDQ
                     TLLNSAVDEAARCVEVLFKDGMVKATNRLNSFKAN"
     gene            complement(9251..10126)
                     /locus_tag="PXE44_000537"
     CDS             complement(9251..10126)
                     /locus_tag="PXE44_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL5588078.1"
                     /translation="MMKKFIPVMIAALVLSACSSQPAKRKSTPRIKATAAYIPTENSA
                     YVVFDLNQKKFLEGKNFNLVRPIASVTKLMTAVVFLEENRYGASCQTQILPSDADFLK
                     GTTSPLPKNLNIACSELLKAMLVRSDNYAAHALAHATHLNKAQFLARMNSKAREIGMT
                     QTYFGDSSGLSSDNVSTVVDLAKLAGYAASKPEIQQLSNSPLVSVYAGGRYFSMKNTN
                     SMVRSQSYQALVSKTGYTREAGYNLAFIAKGDCRGKRIGVVSLNNDNAASRTDFTESM
                     LAKYQCVPNPYRQYD"
     gene            10348..11928
                     /gene="prfC"
                     /locus_tag="PXE44_000538"
     CDS             10348..11928
                     /gene="prfC"
                     /locus_tag="PXE44_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL5588079.1"
                     /translation="MSYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVK
                     GKGSAQHAKSDWMEMEKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTA
                     VDSCLMVIDSAKGVEERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLK
                     IKCAPITWPIGCGKLFKGVYHLYKDETYLYQTGQGHTIQEVRVVKGLNNPELDQAVGD
                     DLAQQLRDELELVQGASHEFDHTEFIKGELTPVFFGTALGNFGVNHFLDGLTEWAPAP
                     QARQSDEREVSPSEEKFSGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIG
                     KDVVISDALTFMAGDRSQAEEAYAGDIIGLHNHGTIQIGDTFTQGENFKFTGIPNFAP
                     ELFRRIRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLINNDLIVGAVGVLQFDVVVSRL
                     KTEYGVEAIYETVNVATARWVEGKDAKKFEEFKRKNEANLALDGGDNLTYIAPTMVNL
                     NLAQERYPDVQFFKTREH"
     gene            12187..13761
                     /locus_tag="PXE44_000539"
     CDS             12187..13761
                     /locus_tag="PXE44_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5588080.1"
                     /translation="MKKWLIPLTVAAFCVSACDDKNSTQATAEKPQVENTQSQKNAEI
                     LPLATVVAPWEINSLDPNQSGVIFQRMNLAETLVEADLNGNLIPALATDWRSNEEGTI
                     WRFTLREGVKFHNDQVLTAQAVVNSLNIAKSKPGVLAKAFIKEIRAVDNKVEIELTKA
                     FVPFPPFLTHYTALILAPESYNEKGEVTQIIGTGAFKPTKIEAPQKLEAVQFEGYWGK
                     KPQVQQANYLASSRSETRMLMAQSEPSSLVFNLDTASVARLKADPNLQLVSGSIARTI
                     QLKMDVAKPFFNDLAIRQALSQAINRKAIAEQVLKIENGAADQILPKAFADWRVDTAE
                     QVVDIAKIKQNLTASGYTFDEQGRLTKDGKPFSFTMRTFSDRPELPVIATILQAQWKQ
                     IGVEVNVSVGNFSEIPAGHQDGSLEMALYALNYGKTLDPYGVIASDFANGGSDWGVMN
                     WQNTEVDNAVKSLETERDPQKAKALKQTVSKIIYNDLPIIPVVYYQQNVVAHKEVKNV
                     TLDPFERRFFLELLSK"
     gene            13981..14913
                     /locus_tag="PXE44_000540"
     CDS             13981..14913
                     /locus_tag="PXE44_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5588081.1"
                     /translation="MHKILFRRLIQILLVIWSVGTLTFILTRQLSGDMAYRIAASRYG
                     YDQADSAAAEAVRAELGLDQPWWQSYFSWLSDLAQFNLGKSLVTGDPVWSEIQHQFGH
                     TLSLAIVALILAILIGPPLGLLAARKPNGFFDRLTLVFSTLFRSVPAFIIAIGLITLF
                     SAQLKWLPAGGYGCWQHFILPALTLAIGLSTVSVRVTRSAMVQVKGSEFYQFSQLKGL
                     SRWHTFLRHGLPNIAVPVIAYHAVQLVYLIEGVVVVESLFAWPGSGHALVHAIIARDV
                     PMIQGTTLVMGGLFVVLNTIADLLSAWIDPRIKR"
     gene            14923..15705
                     /locus_tag="PXE44_000541"
     CDS             14923..15705
                     /locus_tag="PXE44_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5588082.1"
                     /translation="MKISISQYLGGTILLAMLGFAFLQPYFYPMDIGFQDLTNILSNP
                     NEIAWFGTDHLGRDMLARLASAIRLSFGLSLFSVICALIAGLITGIMAGFFGGWIDRI
                     FSFICDLVMALPGLLLVLLFAALSPGSFWTLYLGISLVMWVEFFRVIRSISSTMANSS
                     EIQASRLMGMGWFYSFKRHLLPRLLPMIATLSAFSLGNAILALATLGFVSVGLRPPTA
                     ELGLMMTELFPYYYEAPLIFLQPVVAVFLMVLSLQLLSRKIK"
     gene            15794..17206
                     /locus_tag="PXE44_000542"
     CDS             15794..17206
                     /locus_tag="PXE44_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5588083.1"
                     /translation="MKLLDIQNLSVETENRLQLVEPISLQLKAGKNITILGETGSGKS
                     LLIQAIMGALPDGLIASGEISVKNCKISTESDRLQTLWGKTLVMLPQEPRRSLNPIMA
                     IGKQLWESFHYVAGKPSSQAKTESKAYLAKLGLGESVENYPHQLSGGMAQRASFAIAT
                     AAGGKILLADEPTKGLDPISKGKVIELLKSAYQNGGGLLTITHDIEVAEQLGGTILVM
                     KKGELLEQGEAKALLNNPQHPYTKALISADPKHWQPLQDLQNLSQKQPLVSVKNLSIA
                     RGKKTLFSNLSFDLHKGEVLGIVGHSGIGKSSLADVLCGLLKPKSGEVVWHSHSHKKH
                     QVLKLYQDPPEAFAPNVSLKTLLDDVINKHQLDRLPIPSLLQQLSLNPEILQRNAENV
                     SGGELQRVAILRALLLEPVLLFADEVTSRLDPITQQETIELLINQCRARNCSLILVSH
                     DPYLIEKSCDKVINLEEYVG"
     gene            complement(17293..18036)
                     /gene="rlmB"
                     /locus_tag="PXE44_000543"
     CDS             complement(17293..18036)
                     /gene="rlmB"
                     /locus_tag="PXE44_000543"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL5588084.1"
                     /translation="MSEQIYGIHAVKAFLDNAPERLIEVLVLKGREDKRLMPLLNELQ
                     RLGVAIQQVNRQTLDNKSQGEVHQGIIARVVPQKELNEHDLEAILSQKKNPLLLILDG
                     VTDPHNLGACLRTADAAGVDAVIVPKDKSAQLTPTARKVACGAAEVMPLIRVTNLART
                     MRDLQERHNVWIVGTAGEAESGIYEAKLTGSIALVMGAEGDGMRRLTREHCDQLISIP
                     MAGSVSSLNVSVATGVCLFEIVRQKLAGQ"
     gene            complement(18091..19458)
                     /gene="pssA"
                     /locus_tag="PXE44_000544"
     CDS             complement(18091..19458)
                     /gene="pssA"
                     /locus_tag="PXE44_000544"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDL5588085.1"
                     /translation="MLILNKQRKAEYLLKSLPYLPQVAEQVQFLASSKAFKQQILQLI
                     QTAKKRIYITALYFEKDEAGQEVLAALYQAKRNNPELEINILVDWHRAQRGRIGEAEN
                     LSNADWYAKVKYEQGLPAGQEIAFYGVSINGREVFGVLHLKGFVFDDTILYSGASINN
                     VYLHQFERYRYDRYHVIENKVLADSWVTFIQQHLLTNEAVKRLDTENRPKTAEIRAQI
                     KHFRKQLSREAYLFNGVAENGRLAVSPLVGLGRRKNQLNKVIEALFYQTEQKLTICTP
                     YFNFPHSLRTRIEWLLANGKSVEIIVGDKTANDFYTKPDEKFTMASALPYLYEKNLRA
                     FAKRLDGYVQNKQLTIRLWKDGENSYHLKGVWVDNRYILLTGNNLNPRAWRLDAENAI
                     LISDPQHQLSEKAETELNQICQHTQILTHYSDLEVLTDYPENVRKLLKKFGRVKLDKI
                     VKMLL"
     gene            complement(19528..21075)
                     /locus_tag="PXE44_000545"
     CDS             complement(19528..21075)
                     /locus_tag="PXE44_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HDL5588086.1"
                     /translation="MMKIIAFLFIFLSVFSHTALANSMAEQCVALKSNSPEKTAIYKA
                     EWVEKSAELPAYCLVQGEIGKRVEVDNVPYGIQFELRLPEQWNEKFLFQGAGGVGGVI
                     FPAIGKLYPHGASGANGLNRGYAVVSNDSGHATRDLVFTADPEARSNYAYASIGKVTT
                     VAKQLIAEFYRKPSKHNYIMGCSNGGREAMMAAMRYPQEFDGVIAGSPGFRVSRSVLA
                     EVWDNRALLAVAPKNGDGDKILSQALTQQDLDVIANGVLTRCDKLDGLADGLINAWEQ
                     CDFQPEMVAKQLGQKKVALIKTIFEGAKNSRGELIYSPWAYDSGINAKGWRDWKLGDS
                     QTAQPNSISFKMGLKSLTHYYLAPRVPSRDPLSVDLDQASEQVKAVGSIHDTDQVDLS
                     AFKQNGGKMLIYQGVSDPIFSAVDLKNWYQSLQQAVENPQHFAKLFFVSAMNHCGRGA
                     TVNDFDMLTALENWVEKGLEPESIAAKAGELYPNKAKQIPLCAYPKVAIYQTGEDPNR
                     LTSYQCR"
     gene            complement(21200..24016)
                     /gene="ileS"
                     /locus_tag="PXE44_000546"
     CDS             complement(21200..24016)
                     /gene="ileS"
                     /locus_tag="PXE44_000546"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL5588087.1"
                     /translation="MTIDYKNTLNLPETGFPMRGDLAKREPDMLKNWYDKNLYQKIRT
                     ASKGKKTFILHDGPPYANGNIHLGHAVNKILKDMILKSKTALGFDTPYVPGWDCHGLP
                     IELKVEGIVGKPNEKISAAEFRQACRDYAKEQVEGQKADFIRMGILGDWDNPYLTMNF
                     NTEANIIRTLGKVIANGHLYKGSKPVHWCLDCGSSLAEAEVEYEDKVSPSIYVRFNAV
                     DVNAVEEKFNAQGKGRGALSAVIWTTTPWTIPSNRAIAINPELDYALVQLGDERVLLA
                     VDLVEDVAKAAGVESAEILATTKGENLELLRFNHPFYDYSVPFIFGDHVTTDGGTGLV
                     HTAPDHGADDFVVARKYNIEMAGLISNDGKFKADTPFFAGLGVFESNDKVVEKLQEVG
                     ALLKLSRIKHSYPHCWRHKTPIIFRATPQWFIGMETQGLRQQALGEIKSVRWIPSWGE
                     ARIDTMVANRPDWCISRQRTWGVPMAMFVHNETEELHPRTLELIEEVAKRVEEKGIQA
                     WWDLDPAELLGEEAKDYRKVPDTLDVWFDSGSTYASVVEQRPEFNGNSADMYLEGSDQ
                     HRGWFMSSLMLSTATNGKAPYKQVLTHGFTVDEKGRKMSKSLGNVIVPSEVWNKNGAD
                     ILRLWVASTDYTGEITVSHNILNSAGESYRRIRNTARFLLANLNGFDPKSDLVQPQEM
                     IALDRWAVACALEAQNDIKEAYDNYQFHTVVQRLMRFCSIEMGSFYLDIIKDRQYTTK
                     ADSLARRSCQTALWHIAEALVRWIAPILSFTADEIWGYLPQVEGRSEFVFTEEFYTGL
                     FGLTEQDKLDDAYWQQILKVRAEVNRVLEQARKDKVIGAGLEAKVTVFANDEIRPLLE
                     QLGNELRFVLITSQAIVKPLAEADIAEGELAGLAVKVENADGEKCPRCWHYATDIGSH
                     SGHEEVCGRCVENVAGEGEKRLFA"
     gene            complement(24119..25081)
                     /gene="ribF"
                     /locus_tag="PXE44_000547"
     CDS             complement(24119..25081)
                     /gene="ribF"
                     /locus_tag="PXE44_000547"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDL5588088.1"
                     /translation="MQLIRGFHNLDKHIALNQGCVLSIGNFDGVHLGHQNILARLCDR
                     ALDLGLPSVVMLFEPQPREFFAKKSGNSTACNPPARLMRLRDKLKYLEEAGVDFVLCV
                     RFSEKFAQISADDFISDLLAKRLKVRYLSVGDDFRFGAKRAGSFETLRNAGLKYRFAV
                     EESHTHSLNEERISSSLVREALQNNELDLAEKLLGKPYSILGRVAHGNKLGRTIGFPT
                     ANIMLNRLVTPIQGVFAVKVKTKQGSFNGIANVGNRPTINGTKPLLEVHIFDFNASIY
                     GEAIEVIFLKKLRNEIKFPDFEALKAQIEKDRLQAVEFFKEFAR"
BASE COUNT         7178 a         5762 c         5032 g         7222 t
ORIGIN      
        1 ttcattgcca ttattttttt atggctattt attctagaag gactctaaac gtgttaaaga
       61 aaaattatct cactgtatca attctccttg ccattagtgg agtaggctac gctaatgaaa
      121 tctctttaga gactattact gttgatggaa atacgccttc aaccaaaggg aaattactag
      181 gtggtgaact aaatagcaat gaatctgttg ttgatgaaaa aaattaaaac aaggtagtat
      241 tacattaggt aacgctttat ctggtgaatt aggcattcat tcaagccagt tcggaggcgg
      301 tgcaagtacc ccaattattc gtgggcaaga gagtaaacgt gcaaaaatct tacaaaataa
      361 tggcgaaaac ttagatatgt cgggaatgtc tcctgatcat gcagtaaccg tagatgcgct
      421 acttgctaaa cgtattgaaa ttttacgtgg tccaactact ttactttaca gtgcaggtaa
      481 tacggcaggt gtaattaatg tggtagacaa taaaattcca accgctattc ccgaaaaagg
      541 ctatgagggg caatttggtg tgcgttttgg ctcagccagt aaagagcgtt taacctatgc
      601 aggctcaacc tttgcccttg gaaatcattt agccttgcgg gtacagggaa tgtataacaa
      661 agcttccgaa tactatgctc cacactttac gattgaaggc aaaccttacc accgcgtacc
      721 agatagcgat gtacaatcac aaaccggcac agtaagcctt tcttggattg gagagcgagg
      781 acatcttggt attgcttaca ctgatcgccg tgataaatat ggattaattg gtcacacaca
      841 caaatacgat cactatacta ttagtattat tcgccaagcc gttatgtttg caaaaggtta
      901 tttacgtttt tatccgcacc ttgcagaaga aggagatatt gactataaca accctggtat
      961 ccgtttactg catactcata ttccgggagg ctcacattat ggacaagata ctcacgagca
     1021 cggcaaacct tggattgata tgcactccaa gcggtatgat attgatggaa gtttgcaaaa
     1081 tccacttcct ggttttgaag aagccaaaat atcagctaac tatgtagatt actatcatga
     1141 tgaaaaagat ggaaaaagag ttgaaaatta cttcaagaac aaaggaaaaa atctacgttt
     1201 cgaattggtt cacaaagagt ggaaagggct aaaaggagct attggtgtgc aatataccaa
     1261 ccaatctacc tcagcacttg cacttgaagc ttcacgagct gcaaaagtat ttaataaaca
     1321 gcctttactc aataatccaa aaacaaaatt atggagtctc tttgcgattg aacgccttaa
     1381 tttaggtgac tttacctttg agttaagtgg gcgggcagaa cgtcaaaaaa tagcaatgga
     1441 ttatgatgtg aaattaattg atcgctggct aggctttaat acacctatgc caaacttgga
     1501 cccacataaa gacaaaggtt attcttatag ctttgcaact cattggtatt tcgcaccaaa
     1561 ccataagtta acattaaatg ctgcacatca agaacgttta cctaatgctc aggaactcta
     1621 tgctcatggt aaacacattg ctttaaatgc atttgaggct ggcaataaaa atctaaaaaa
     1681 agagcgttct aaccaaattg agctatcatt agcttatgtt ggcgacaaat gggattataa
     1741 attaaatctc taccacaccc gctatggcaa ctatatttac ccattaactc ttaatgataa
     1801 tcgtggacct aaatctttta ccgatgaata taacttgaaa gtgaatagat attatcaagg
     1861 cgaagctcgt tttagtgggg cagaaggcga aattggttat ctatttacac caaattatcg
     1921 cttagccgta ttcggagact atgtccgtgg aaagttagtc aacttaccta atattgcaat
     1981 gagttacaac atttggacag gtgaagttga caaatgggct tctcagccag acatttctgc
     2041 acctcgtata ccaccactac gtttaggagc aagatttaat gctgacttca acctaaactg
     2101 gtctggaatg ttagaatatt accgagtatt tgctcagaaa aaagtctcga aatacgaaca
     2161 agttacccct ggccatcatc aagtaaacct tggcgtaacc tatagtaatc attttaatca
     2221 aacagaatac caagtattcc ttaaagtgga taacttgctt aatcagaaaa tgtaccaaca
     2281 tgcctcatat ctaccacata ttccacaaat gggtagaaat gcaatgcttg gaatgaatat
     2341 cagtttttaa tttgtaaatc tctacaaaaa tataaccgct tgtaaatttc tatctacaag
     2401 cggttacttt cagtggattt tttgcaatta gagatcgtca attaccaagt aaattttatt
     2461 taccgggccg tgaacgccaa ctactttaat caactcaata tccgcagtcg ccgatgggcc
     2521 tgagatgata ttcacacaag atggcattct ttcacctcgt tgggcttttt cgtgcagaat
     2581 ttgtgcaagc tgtgccacac gaggtaacac tgtacttttg cgtaacacaa ccaccgattt
     2641 ttcgggcaat aagctcactg aacgcccaaa ttctttatcg gaaaacagta caataccgcc
     2701 agactctgcc aagccatatt caccgtaaac cacaccgata ttagcagttc tggctttttc
     2761 aaagttggtt tcagcttggt tgaaatccca cacatagctg tcttcgtatt ttgcttgaat
     2821 ggctgcggta atcccaagat cctttaaacg ttgatcgtta ttcacgatca cactcgcccc
     2881 gccatatttt tcacaaatat caataatggc ttgtggtaaa tccgcctctt ttgcctctac
     2941 tacatctgcc atcatcactc gggcaaaatc gataaactct tggcaaagtt gtggttgagg
     3001 gcgatcggtt aaacgggtaa tcgcgtggtt gttttccaac acaggaattg gctcaggcac
     3061 attttggcgt ggtctgccca tacgctgtgc caatttattt aagaaatttt cacgattttg
     3121 taaatccatt ttcttatcct ctgttattaa accattcacg gaaactttcg ccttcggcag
     3181 tcggtaaatc acgggctttc gtccattcac ctaacgcccc aaaattcacc ggtgctttgc
     3241 cgtttttaat caatttgctt gccactttcg cccccacttt tacgccgact ttccaaaggg
     3301 ttgggtgtga gttagcgaag ttaaagccaa aaatagacaa gcgttctgcc attggcgtta
     3361 agccggcatc cgcaattttc tcacggtgtt ttaagataag ctgtgccagt ggaattttca
     3421 ctggacaaac actgttacaa gcggtacaaa gtgagcaagc atacggcaat tctttaaact
     3481 cttcgtagcc accgagcaat ggcgaaataa ctgaaccaat cgggcccgga tagattgaac
     3541 catagccgtg accgccaatt tggcgatacg ccgggcaagt gttcaaacat gcaccacagc
     3601 ggatacaacg caacacttct tggaattcgc tttctaagat tttagaacga ccgttatcga
     3661 caatgaccaa gtggaactct tccggaccat cggtttcgcc ttcaagtcta ggacctgtca
     3721 gccaagtgtt gtaagcggtg agttttgccc ctaccgcact gcgagccagc atggtaatta
     3781 atacatcaac ctctttaaag gttggggcga tacgttccat acccattact gcaatatgcg
     3841 ttttcggcac agttgttgcc atacgcagat taccttcgtt ggtcactaaa caaaccgagc
     3901 cggtttccgg caccgcaaag ttacagcctg aaataccgat gtctgcctct aagaaatctt
     3961 cacggatttt ttggcggacg aatgcagtca tctcttcagg tgtttccgaa ccttgataac
     4021 ctagcacatc gtgcatttct tggcggattc tgtggcgatc tttgtggatt gcaggtacaa
     4081 caatatgaga aggtttatcg cccacaattt gcaataaata ttcgcccaaa tcggtttcaa
     4141 ccactttaat gccttctgct tctaaaacgt ggttcatccc aatctcttcg gttaccatcg
     4201 acttcgattt tacgattttc ttcgcatttt tagctttcgc cacgtttttg atgtaatcag
     4261 tggcttcttc ggcggtttcg gcaaagaaaa cgtgtccgcc gttttgggtg actttttcgc
     4321 ttaattggta gagataagcg tctaaattgg ctaaaacgtg gttacgaatt gctttcgcat
     4381 catcacgcca ctcttcccag ttgcccagct catccaccat acgctgacgg tttgcaccga
     4441 ttgtttcctg agccttaact aaggctttac gcacaatctc gttattaact tgctgctcaa
     4501 cacgttgttt aaacggcagg gtgttagttt gtaaataaga catcttattt tcctccctgc
     4561 attaacactt cggcaatgtg catcactttg attggcttac cttcacggct taaacgaccg
     4621 ccaatgttca tcaagcagct tacatctgcc ccgattaaat aatccggctc atcggtatct
     4681 acatttgcga ctttctcttt taccatctcg ccggaaattt ctgccatttt gaccgagaat
     4741 gtgccaccaa aaccacagca agtttcttgg tttttgattg gtaataactc taacccttta
     4801 acgtgctgta ataaagcaat aggctcatct ttaatcccca gcttacggaa taagctgcaa
     4861 gacgggtgat aaactgcttt gcccggtaaa taagccccaa catctttcac gcccagtttg
     4921 ttcacaataa aatcggttaa atcatagaaa cgatcagcca ctttcttcgc acgttctgcc
     4981 cattcttttt cgccaaagcg ttcaaaatga tccggatagg ttttgatcgc atatacacaa
     5041 gaacctgccg gggcgacaat cgggtaatca ttagcttcaa aggtttcaac caagtttttc
     5101 atacctgcta aagcttgctt agtgtaaccg ctgttgatcg caggttgtcc acagcaacct
     5161 tgtttttcta agaaagtgat tttacaacct aatttttcca ataaaagcac agtatttttt
     5221 gcaacccctg ctttcattac atctgcaatg caggttacgt aaaaattaac gttcataaag
     5281 aactcccaca gtaaatataa ataagaatta aactcaaaaa atgccgttct aagacggcga
     5341 aaggaagagg tggatttgca aaaaatttgc taaatctcac cgcttccttt ctaaatcaag
     5401 taagctgatt attaactcat tataggttat agaacatagg tactataaac aatgccgaaa
     5461 tcgcagcaat aacgccatac acgaacattg gcacggctgt tttcttaata atattgcctt
     5521 cttgtttctc aatgccggta acagaacaca cagccacgat gttgttaata cacaccatat
     5581 tccccattgc accaccaact gattgcaatg caagaattaa ggtaacagat aacccgactt
     5641 gctgtgccgt tgcatattgt actgttccga aggttaagtt agataccgta tttgaccctg
     5701 agaagaacgc accaattgca cctaagaacg aggcaaataa tgtccaagat tgaccggtaa
     5761 attctgcgaa gtttttacca atgatctgaa ccattgagtc tacaccacca attaacatta
     5821 attttaccat cacaatggca cctaaaagtg ctaagaatgg ttttttcact tgtttaaatg
     5881 aagtgccaaa aatctcttga gtatttttac ctgataccga gaaaatcggt aagcaaatta
     5941 atacggtcac aacaaatgga atcaacgcag gaacatacag taatttatag ctagttgcta
     6001 cattcgtacc taaaatattt ttcagtgcaa aaattaagcc ttggctaatt tcaaagttag
     6061 cgaaaccaag cgaactgaca atccaagctg ttgcatcatt tagcattgct tttaatggaa
     6121 gttgctgaat acgggtgatt accaaaatga agatcagtaa ccctgttggt aataatgctt
     6181 ttgccacttc accaaaggtc gctttatcgc cgtcaaggtg gttctcgctt ttcgctaaac
     6241 caatacccat atttgctacc cagactgaca ccaataaacc aatcgccccg gcaactaatg
     6301 atgggaattc gtagttaaat tgagcaataa tgaagtaagg aatgacacag gcaaaaatgc
     6361 tgatataaat aaacacgata ttttgacgaa tttctttcca gctcacaatt aaacgcaatg
     6421 ccataactgg aataataaac gcagcaatag agtggatcag tgcagtaata gaaccaattt
     6481 caagcacttg tgcatcggtt agtaaaccat cttttaataa tggtcccata ccaaaccaag
     6541 ttggtgtgcc aaccgcacca aatgacaccg gcacagagtt cattactaag gctaacattg
     6601 ccacttgtaa cggtttaaag cctaaaccaa ccagaattgg agctgcaatt gccgccggcg
     6661 taccgaaacc tgatgcgcct tcaatcataa atgcaaatgc ccaaccgata atcatcaact
     6721 gtgccaccgg attcgggtta attgtcccta accatttacg catagtgttg gttgcacctg
     6781 aaacttcaga gaaacggtta aataaaatcg ccccgaaaat aacggtaatt ggggttaata
     6841 catcacctaa accggcaata atattggcaa agattaacgt ggtatcatta ccaaaatagg
     6901 taagctgaag cacaaaaatt aataacgccg taagtggtaa agccacataa gacggtaacg
     6961 cgttacgttt taccattaaa taaatgagca aaacaattgg aaatacgctc aggaatagag
     7021 ccatactgca cctctttata tgattataaa atgacctaac tggtcggata ggtgcgaatt
     7081 gtactcttaa aaaaaactaa tgtaactagg ttgttacaat tttgtgatct agctcgcatt
     7141 tttaatgatt gtatggttaa aaaagagcca tatttatttt catttaatac ttaatagtta
     7201 gccctattta agacattttt accgaatcga gcaatatgca attaattaac aaaaaaaggc
     7261 cgcttgtaga tcacagatca acaagcggtc ttttttaggc aaaaatttgc aaattaaaca
     7321 ttgaaacgga agtgcattac atcgccgtct tgaacaatat aatctttacc ttccaaacgc
     7381 catttacccg cttcttttgc accggcttca cctttattgt ctatgaagtc ttggtaagca
     7441 attacttccg cacgaataaa gcctttctca aagtcagtgt gaatcacggc tgctgctttt
     7501 ggagcggttg cacctaccga aaccgtccac gcacgcactt cttttacccc tgcggtaaag
     7561 taggtttgta agttaagtaa acggtagcct gcacgaataa cacggtttaa gcccggctct
     7621 tcaatgccta aatcttgtag aaattccact ttttcttcgt catcaagctc ggcaatttcc
     7681 gattcaatcg ctgcacacac cggtacaacc accgcccctt ctttttcggc aatttcacgc
     7741 acacggtcaa gataagggtt attttcaaaa ccgtcttcgt tcacgttggc aatatacatt
     7801 gtcggtttta aagttaagaa gttgtagccc ttaatagcgt gcagttcatc cttatctaaa
     7861 tctaccgaac gaatcatacc ggcattttca agcacaggta ggattttttc cataatcgat
     7921 aattcaaatt ttgcgtcttt atcgccacct ttcgcacgtt tttgtaaacg ctgaatggca
     7981 cgctcgcaac tgtctaaatc ggcaagtgcc aattcggtat tgatgatttc aatatcatca
     8041 gctgggtcaa ttttccccgc aacgtgaacg atgtcatcat tttcaaaaca acgcacaacg
     8101 tggccaatcg catcagtttc acgaatattg gctaagaatt tattacctaa accttcgcct
     8161 ttgcttgcac ctgccactaa gccggcaata tccacaaatt ccattgtagt tggtaacact
     8221 cgctcaggtt tcacaatttc cgccaacgca tctaagcgag ggtcaggcat tggtaccaca
     8281 ccggtatttg gctcaatcgt acagaacgga tagtttgctg cctcaatacc tgctttagtt
     8341 aatgcattga aaagggtcga tttaccgaca tttggtaaac ccacaatacc acatttaaat
     8401 cccatttttt gttccttaga attcccctct ccccttgcgg gagagggata aatttgagct
     8461 tcgttcagaa gcgaaaatga gggagagggc gaaattgcaa taatttcctc aaatatgacc
     8521 gctcccctct ctctgaaaaa tacttctgaa cgaagtattt ttcggtctct ctcccacaag
     8581 ggagagagtt ttaatttgct ttaaaactat ttaaacggtt agttgcctta accattccgt
     8641 ctttaaataa aacttctacg caacgtgcgg cttcatcaac cgcactattc agtaatgtct
     8701 gatcgcttgg tgaaggcttg cccagcacat aattcgccac taagtcttta tgccccgggt
     8761 gtccaatacc aattcgcaca cggtaaaact ctttattatt gccaagggcg gcaataatat
     8821 ctttcaagcc gttatgacca ccgtgtccgc cgcctttttt caatttcacc gaaccgggtg
     8881 gtaaatccaa ttcatcgtga gcgaccaaaa tttcttccgg tttaatccga taaaaattgg
     8941 ctaacgctcc cactgcttta ccacttaaat tcataaaagt agtcggcact aataaacgga
     9001 cttcgccttg agcggtatta atttttgcta ctttgccaaa atatttcggc tcttctttta
     9061 ggctaacgtt atatattcgg gcaagttcgt taatcagcca ctcgcctgca ttatggcggg
     9121 tttcttcata ttttaccccc gggttcgcca gcccgacaat taatttaatt tctgacacaa
     9181 tcttgtctca attcatttaa tttaaaaggc agtattttag agaaaaaccg ctattttctc
     9241 aaggcaacca ttaatcatat tgtctatacg gatttggtac gcattgatac ttcgctaaca
     9301 tcgattcagt gaaatcggtg cgtgaagcag cgttatcatt gtttaaacta accacaccaa
     9361 ttcgcttgcc tctgcaatcc cctttagcaa taaaggcaag attataaccc gcctcacggg
     9421 tataacctgt cttactcact aatgcctgat aggattggga acgaaccata ctgttggtgt
     9481 ttttcataga gaaataacga ccacctgcat aaacactcac taatggcgaa ttagacaatt
     9541 gctgaatttc aggcttactt gccgcatagc ctgccaattt agcaagatca accaccgttg
     9601 aaacattatc cgaagataag cctgagctat caccaaagta ggtttgagtc ataccgattt
     9661 cacgggcttt gctgttcatt cgagccaaaa attgggcttt attcaaatgt gttgcgtggg
     9721 caagggcgtg agcggcatag ttatcggagc gaactagcat cgcttttaac aattcactac
     9781 aggcaatgtt aagatttttc ggcaatgggc tggtcgtgcc ttttaagaaa tcagcatctg
     9841 acggcaaaat ttgggtttgg caactcgccc catagcggtt ttcttctaag aaaacgactg
     9901 ctgtcattaa ttttgtgacc gaagcaatcg ggcgaactaa attgaaattc ttaccttcca
     9961 aaaatttctt ttggtttaag tcaaacacca cataagcgga attttcggtt ggaatataag
    10021 cagcggttgc tttaatccgt ggtgtgcttt tcctttttgc cggctgggag gaacaggcgc
    10081 ttaacaccag agccgcaatc ataacaggaa taaatttttt catcataata gattcatcga
    10141 taataaagtc aaagctggtt attctaccct gtttccaaaa ttgtgccaaa gcaagcggtc
    10201 gatttttaca aaatttttgc aaatctagcc gattttgcaa aatttccgcc aaatttaacc
    10261 gcttgccctc tcgcctcaaa tcagcgaaaa taacgcttaa ttttttggcg gactttccgc
    10321 cctttttatt ttaattggtt gagaaaaatg tcatacccac aagaagttaa taaacgtcgc
    10381 acctttgcga ttatttccca ccccgatgcg ggtaaaacca ccattaccga aaaagtattg
    10441 ctctacggaa atgcaatcca aactgccggc tcggtaaaag gcaaaggctc ggcacaacac
    10501 gctaaatccg actggatgga gatggaaaaa caacgtggaa tttcgattac cacctccgtg
    10561 atgcagttcc cgtacaatga ttgcttggta aacttactcg atacaccggg gcacgaagac
    10621 ttctcggaag atacttatcg tactttgact gcggtagata gctgcttaat ggttatcgac
    10681 tcggcaaaag gggttgagga acgcaccatt aaattaatgg aagtgacccg cttgcgtgat
    10741 acgccaatcc taacctttat gaacaagcta gaccgtgata ttcgtgaccc gatggagctg
    10801 ttagatgaag tggaaagcgt gctgaaaatc aaatgtgcgc cgatcacttg gccgattggt
    10861 tgcggaaaat tgtttaaagg ggtatatcac ctttacaagg acgaaaccta cttataccaa
    10921 accggacaag gtcataccat tcaagaagta cgagtggtta aaggcttaaa taatccggaa
    10981 ctggatcaag ccgttggcga tgatttagcc cagcagttgc gtgatgaatt agagctggta
    11041 caaggcgctt cgcacgaatt cgatcacacc gaatttatca aaggcgagct gacaccggtt
    11101 ttctttggca ctgcactggg taacttcggg gtcaatcatt tccttgacgg tttaaccgaa
    11161 tgggcaccgg caccacaagc ccgtcaatcg gacgaacgtg aagtttcacc aagcgaggaa
    11221 aaattcagcg gtttcgtatt taaaattcaa gcgaatatgg atccgaaaca ccgagatcga
    11281 gttgctttta tgcgtgtcgt ttcaggtaaa tatgaaaaag gaatgaagct taaacacgtc
    11341 cgtatcggca aagatgtggt gatctccgat gccttaacct ttatggcggg cgaccgttca
    11401 caagccgaag aagcctacgc cggcgatatt atcggcttgc acaatcacgg cacgatccaa
    11461 attggtgata ctttcaccca aggcgaaaat ttcaaattca ccggtattcc gaacttcgcc
    11521 cctgagctgt tccgccgaat ccgcctgcgt gatccgctca aacagaaaca actgctcaaa
    11581 ggcttggtac aactttccga agagggtgcg gtgcaagtat tccgtccgtt aatcaacaac
    11641 gatttgatcg tgggagcggt tggggtgcta cagtttgatg tggtggtttc acggctgaaa
    11701 accgaatacg gtgtagaggc aatttatgaa accgtaaacg ttgccacagc ccgttgggtc
    11761 gaaggcaaag atgccaaaaa atttgaagag ttcaaacgca aaaacgaggc aaaccttgcc
    11821 ctagacggcg gagacaacct cacctacatt gctccgacaa tggtgaactt aaacctcgcc
    11881 caagaacgtt accctgacgt acagttcttt aaaacgagag agcactaaaa ccaatagggg
    11941 cgaaaaatct ttcgccccta caaataaagc acaagcggtc aatttttttt ccgaatttta
    12001 caatactagg cattctgttc ctcaaatagc ctaaatattc ccctatcaac aatccttatc
    12061 ttgagattat tctgcctatt aattaaccac tcaaaaatac cctcgcagaa aactctactt
    12121 tttttatgga aatatgctaa agttcgccaa tattcatagc cctgattttt ttatggagaa
    12181 aataaaatga aaaaatggtt gattccatta actgtcgcag cgttctgtgt ttctgcttgc
    12241 gatgataaga acagcactca agcaaccgca gaaaaaccgc aagtagagaa tactcaatcg
    12301 caaaaaaatg cggaaatatt accgcttgca accgtggttg ccccatggga aatcaatagc
    12361 cttgacccga atcaatcagg cgtaattttc cagcgtatga acttagcgga aacattggtc
    12421 gaagccgatc ttaacgggaa tttaattccg gcattggcca ctgactggcg cagtaacgaa
    12481 gagggaacaa tctggcgttt taccttgcgt gaaggggtaa aattccataa cgatcaagta
    12541 cttaccgcac aagcagtggt aaatagtttg aatatcgcaa aaagcaaacc gggtgtgtta
    12601 gcaaaagcct ttatcaagga aattcgagcg gtagataaca aagtggaaat tgagctaact
    12661 aaagcttttg tgccgtttcc tcccttttta acacactaca ccgccttaat tttggcacct
    12721 gaaagctata atgaaaaagg cgaagtgacc caaattatcg gtacaggtgc gtttaaaccc
    12781 acaaaaattg aagctccgca gaaattggaa gcggtgcaat ttgagggcta ttggggcaaa
    12841 aaaccgcaag ttcagcaagc caattattta gccagcagcc gtagcgaaac ccgtatgtta
    12901 atggcacaaa gcgagccgtc atcactggta tttaatttag ataccgccag tgtagcaagg
    12961 ctgaaagccg atcctaacct gcaattagtc agcggatcta ttgcccgtac tattcagttg
    13021 aaaatggacg tggcaaagcc gttttttaac gacttagcga ttcgccaagc cttatcgcaa
    13081 gcgattaatc gcaaggcgat tgctgagcag gtcttaaaaa ttgagaacgg agctgccgat
    13141 caaattttac cgaaagcctt tgccgactgg cgtgttgata ccgcagaaca agtggtcgat
    13201 attgcaaaaa ttaaacaaaa tctgaccgct tccggctata cctttgatga acaaggtcgc
    13261 ttaaccaaag acggcaaacc gtttagcttt accatgcgca ccttctcaga tcgtcctgaa
    13321 ttaccggtga ttgctactat cttacaagct caatggaaac aaattggcgt ggaagtgaat
    13381 gtttcggtgg gtaatttcag cgaaattccg gcggggcatc aagacggcag tttagaaatg
    13441 gctctttatg ccttaaatta tggcaaaacc ttagatccat atggcgtaat tgcctccgac
    13501 ttcgccaatg gtggcagtga ttggggggta atgaattggc aaaatactga ggtagacaat
    13561 gcagtcaaat cgctcgaaac cgagcgtgac ccgcaaaaag cgaaagcatt aaagcaaacg
    13621 gttagcaaaa ttatttacaa tgatttgcca atcattccgg tggtttatta ccaacaaaat
    13681 gtggtggcac ataaagaagt aaaaaatgtg acgctagatc catttgaaag acgcttcttt
    13741 ttggagttat taagtaagta gtttaattca aaatactcct cgctttagaa aagggaaggt
    13801 aggatttttc agtagtaatt tgcgctctta ggtgaataaa tagtgacaga attatcgttt
    13861 tttctcattt ttcacctatt ttctccgctt atttcttctg ccaaatcttc ccttcccctc
    13921 tttgctaaag agggggatca gaacgcataa aatcatcttt ttaataaatg aaataataca
    13981 atgcacaaaa ttctcttccg ccgtcttatc caaattctac tggtgatttg gtcggttggc
    14041 acactcacat ttatcctcac ccgccaactt tcgggtgata tggcataccg cattgctgcc
    14101 agccgctatg gttatgatca agcagatagt gctgctgctg aggctgtgcg tgccgagctg
    14161 ggattagacc aaccttggtg gcaaagctac ttcagctggc taagtgactt ggctcaattt
    14221 aatttaggca aatcattggt gaccggtgat ccggtctggt ctgaaattca gcaccaattc
    14281 gggcatacct taagccttgc gattgtggca cttatcttag caatccttat cgggcctccg
    14341 ctcggtttgt tagccgcccg taagccaaac ggcttttttg accgcttgac actggtattc
    14401 agtaccctgt ttcgctccgt gccggcattt attattgcga ttggcttgat tacgctgttt
    14461 tccgctcaac taaaatggct acctgccggc ggatacggct gttggcaaca ttttatcttg
    14521 cctgccttaa cgcttgcgat tggcttgagt acggtttcgg tacgagtcac ccgttctgca
    14581 atggttcagg taaaaggctc agaattttac caattttcac aattaaaagg cttaagccgt
    14641 tggcatacct ttttacgtca cggtttgccg aatattgccg tgccggtcat tgcctaccac
    14701 gcagtacaat tagtttattt aattgaaggc gttgtggtgg tcgaaagcct atttgcttgg
    14761 cccggcagcg gacacgcact ggttcacgcc atcattgccc gtgatgtgcc gatgattcaa
    14821 ggtacaacgc tcgtgatggg cggattgttt gttgtattaa acaccattgc cgatctatta
    14881 agtgcttgga ttgacccacg aattaagcga taggacatca agatgaaaat ttcaatttca
    14941 caatatttag gcggcactat tctccttgca atgttgggat ttgccttttt acagccttat
    15001 ttttacccga tggatattgg ctttcaagat ttaaccaata tcctttccaa tccaaatgaa
    15061 atcgcttggt tcggcaccga tcatctcggg cgggatatgc ttgcccgtct tgcctctgct
    15121 attcgtcttt cgtttggctt atcactgttt agcgtgattt gtgccttaat tgcaggctta
    15181 attaccggca ttatggcagg ctttttcggt ggctggattg accgcatttt cagctttatt
    15241 tgtgatctag tgatggcact ccccggctta cttttagtat tgctctttgc cgccctttct
    15301 cccggctcat tttggacgct ctatttaggg atttcattgg taatgtgggt ggaatttttc
    15361 cgtgtgatcc gctctatcag ttcaacaatg gcaaacagct cggaaattca agcctcacgc
    15421 ctaatgggaa tgggctggtt ttatagcttc aaacgccact tattgccccg tttattaccg
    15481 atgattgcca ctttatcggc attctcgctc ggcaatgcga ttttagctct cgccacactt
    15541 ggctttgtca gcgtagggct tcgcccgcct acagcagaat taggcttgat gatgaccgaa
    15601 ctattccctt actactacga agcaccgctg atatttttgc aaccagtggt tgccgttttc
    15661 ttaatggtac tcagcttaca attattgtct agaaaaatta aataatgcgt aggggcaggt
    15721 cctgtgcctg cccaaaattc ttattatatt tatgggcggg cacagggccc gcccctacag
    15781 taataaaatg aagatgaaat tactcgatat ccaaaaccta tctgttgaaa ccgaaaacag
    15841 attacagctg gtcgaaccga ttagcctaca attaaaggca ggaaaaaata ttactatctt
    15901 aggcgaaacc ggctcaggca aaagcctgct gattcaagcg attatggggg cgttacccga
    15961 cggcttaatc gcaagcggtg aaatttcggt aaaaaattgc aaaatttcca ccgaatccga
    16021 ccgcttgcaa acattgtggg gtaaaacctt ggtaatgctg ccacaagagc ctcgccgctc
    16081 gcttaatccg attatggcaa tcggcaagca gttgtgggaa agttttcact atgtggcagg
    16141 caaaccatca tctcaagcca aaacggaaag caaagcctat ttagccaaac ttgggctggg
    16201 cgaatcggtt gaaaactacc cacaccagct ttccggtgga atggctcaac gtgcctcttt
    16261 tgcgattgca actgccgccg gtggcaaaat tttacttgcc gatgagccga ccaaagggct
    16321 tgacccaatc agtaaaggca aagtgattga actgttaaaa tccgcctacc aaaacggtgg
    16381 aggcttgctc accattactc acgatattga agttgccgag caactcggtg gcactatttt
    16441 agtgatgaaa aaaggcgaac tgttggagca aggtgaggca aaagcgttgt taaataaccc
    16501 acaacaccct tacaccaaag cacttatcag tgccgatcca aaacattggc aaccattgca
    16561 agatctgcaa aatttaagcc aaaaacaacc gcttgtttcg gttaaaaatt tgagtattgc
    16621 ccgtggtaag aaaacgctgt ttagcaacct ttcctttgac ttacacaaag gcgaagtgct
    16681 tgggattgtc gggcatagtg ggattggcaa aagctcgctt gccgatgtac tttgtggttt
    16741 gcttaagccg aaatcgggcg aggttgtgtg gcacagccac agccataaaa aacaccaagt
    16801 gttgaagctt taccaagacc caccggaagc ctttgctccg aatgtcagtc taaaaacctt
    16861 gcttgatgat gtgattaaca agcaccaatt agatcgactg ccgattccaa gtttactgca
    16921 acaactttcg ctcaacccag aaattttgca acgcaatgcg gaaaatgtct ccggcggcga
    16981 gctacaacga gtagcaattt tgcgagcctt gctcttagaa ccggtactac tgtttgccga
    17041 tgaagtcact tctcggcttg acccaatcac acaacaagaa acgatagaat tgctgatcaa
    17101 ccagtgccga gcgagaaatt gcagtctgat tttggtcagt cacgatcctt atctaattga
    17161 gaaatcgtgc gataaagtga ttaatttgga agagtatgtg ggctaacaag cggttcaatt
    17221 tctgagattt tttgcggctc taaaaacaaa aatgcgaaga catcagttct tcgcattttt
    17281 cttacttatt ccttattgcc cagccagctt ctgccgtaca atttcaaaca gacagacccc
    17341 ggttgcaact gagacattca aagaagatac tgagcccgcc attggaatgc tgataagctg
    17401 gtcgcaatgc tcacgggtaa ggcgacgcat tccgtcgcct tctgccccca taactaacgc
    17461 aatcgagccg gttaattttg cttcataaat gcccgattcc gcctcacctg ccgtgccgac
    17521 aatccagaca ttatgccgtt cttgtaaatc tcgcattgtg cgagccagat tggtaactcg
    17581 aatcagtggc atcacttccg ctgccccaca ggcgactttg cgagcggttg gtgtaagctg
    17641 agcagattta tctttcggca caataaccgc atcaacccct gccgcatcag cggtacgcaa
    17701 acaagcccct aggttgtgag ggtcggtcac gccatctaaa atcaacaaga gtggattttt
    17761 cttttggctt aagatcgcct ctaaatcgtg ttcattcagc tctttttgtg gcacgactct
    17821 ggcaataatc ccttgatgca cttcaccttg cgatttgtta tctaaggttt ggcggttcac
    17881 ttgttgaatt gccaccccta aacgttgtaa ttcatttaat agtggcatta agcgtttatc
    17941 ttcacgccct tttagcacca gcacttcaat taaacgctca ggtgcattat ccaaaaaggc
    18001 tttcactgcg tgaatgccat aaatttgttc gctcatattt tatccttaat aattgggcga
    18061 acccatcagc tcgcccatat aaatacctaa ctaaagcaac atcttcacaa ttttatccaa
    18121 cttaaccctg ccgaattttt tcagcaattt acgcacattt tcagggtaat cggttaaaac
    18181 ttccaaatca ctgtagtgag ttaaaatctg cgtatgttgg caaatttggt ttaactccgt
    18241 ttctgctttt tcacttaatt gatgttgagg atcggaaatt aaaattgcat tttcggcatc
    18301 taaccgccac gctcttgggt taaggttatt gccggtcagc aaaatatagc ggttatccac
    18361 ccaaacccct tttaagtggt agctgttttc gccatctttc cataagcgaa tggttaactg
    18421 tttattttgt acataaccgt ccaaccgttt tgcaaaggct cgtaagttct tttcatacaa
    18481 ataaggcaga gccgatgcca tcgtgaattt ttcgtccggc ttggtgtaga aatcattagc
    18541 ggttttatcc cccacaataa tttcaaccga tttgccgttt gccaatagcc attcaatacg
    18601 ggttctcaga gaatgcggaa aattaaaata cggagtgcaa atcgttaatt tctgctcagt
    18661 ttgataaaac aacgcttcaa tcactttatt taactgattc ttacgtcgcc ctaagcctac
    18721 taaaggagaa accgctaagc gaccattttc agcaacaccg ttaaataaat acgcttcccg
    18781 acttaattgt ttgcggaaat gcttaatttg cgcacgaatt tcagccgttt tcgggcgatt
    18841 ttccgtatct aaacgtttta ccgcttcatt ggttaaaaga tgctgttgaa taaacgtcac
    18901 ccagctatcg gcaagaactt tattttcaat gacgtggtaa cggtcataac ggtaacgttc
    18961 aaattgatgc aaatacacat tattaatgct tgctccgcta tacaaaatcg tatcgtcaaa
    19021 tacgaagcct ttcaaatgca atacaccaaa cacttctcgt ccgttaatcg acacaccgta
    19081 aaaggcaatc tcttgccctg ccggtaaacc ttgttcgtat ttaacttttg cataccagtc
    19141 ggcgttagat aaattctctg cctcaccaat acgcccacgt tgggctcggt gccaatcaac
    19201 caaaatgtta atttccagtt caggattatt tcgcttcgct tgatataaag cagctaatac
    19261 ttcctgcccg gcttcatctt tttcaaagta aagagcggtt atataaatac gcttttttgc
    19321 cgtttgaatc agctgtaaaa tttgttgttt aaacgctttg ctcgaggcga gaaactgaac
    19381 ctgctctgcc acttgtggta agtatggcag gcttttcaat aggtattcag ctttacgttg
    19441 tttgttcaaa attaacatca ttgattccaa aattttcaat cgttgcctgc tattttacac
    19501 tgaaatcgcc aataaagcga ttttcgctta acggcattga tagctggtca aacgattagg
    19561 atcttctccg gtttgataaa ttgcgacttt cggataagca caaagcggga tctgcttggc
    19621 tttattaggg taaagctcac cggctttggc ggcaatactt tccggctcca gtcctttctc
    19681 aacccaattt tcaagggcag tgagcatatc aaaatcattc accgttgccc ctcgcccaca
    19741 atggttcatt gccgacacga aaaacaattt tgcaaaatgt tgcggatttt cgaccgcttg
    19801 ttgtaggctt tgataccaat ttttgagatc aactgccgaa aaaatcgggt ctgaaacgcc
    19861 ttgataaatc agcattttgc caccgttctg cttaaaagcg gataaatcaa cttgatctgt
    19921 atcgtgaatg ctccctaccg ctttcacttg ttcgctggct tgatccaaat ctacgctgag
    19981 tggatcacgg ctcggcactc tgggggctaa atagtaatgg gtcaaacttt tcaatcccat
    20041 tttaaaacta atgctgttgg gctgagcggt ttggctatca cctaatttcc aatcccgcca
    20101 gcctttagcg ttaatgccgg aatcatacgc ccaagggcta taaatcagct cgcctcgact
    20161 gtttttcgcc ccctcgaaaa tcgttttgat taaagcgact tttttctgcc ccagttgctt
    20221 tgccaccatt tccggctgga aatcacactg ttcccacgca ttaattaaac cgtctgctaa
    20281 gccgtctaac ttatcgcaac gtgttaatac gccattggca atcacatcta aatcttgttg
    20341 agtgagggct tgggataaaa ttttgtcgcc atcaccattc ttcggagcaa ctgccaacaa
    20401 ggctcgatta tcccatactt ccgccaagac tgaacgggaa acccgaaagc cggggctgcc
    20461 tgcaatcaca ccgtcaaatt cctgtggata acgcattgcc gccatcatcg cctcacgacc
    20521 accattcgag cagcccataa tgtaattgtg cttgctcggt tttcggtaaa attcggcaat
    20581 caactgcttg gcaaccgtgg tcactttgcc gatagaggca taggcataat ttgagcgagc
    20641 ttcgggatct gcggtaaaca ctaaatctcg agtggcgtga ccactgtcgt tactcactac
    20701 tgcatagcct ctgtttaagc catttgcacc actcgccccg tgcggataga gcttaccaat
    20761 ggcagggaaa atcaccccac ctacaccgcc cgccccctga aataagaatt tttcattcca
    20821 ttgttcgggt aaacgtagct caaattgaat accgtaagga acattatcca cctccacccg
    20881 ttttccaatt tcaccttgta ctaaacagta agccggcaac tcagcagatt tctcaaccca
    20941 ttcagcttta taaatagccg ttttttccgg cgagttagat tttaacgcga cacattgttc
    21001 agccatagaa ttagccaaag cagtgtgaga aaaaacggag agaaaaataa aaagaaaagc
    21061 gattattttc atcatttaca tcaatacaag cggttatttt tccgaaaatt tttgcaaatt
    21121 ttaaactgag tgtctatcta tcgctggaaa agctaaggca gagtttcctc tgccttagaa
    21181 agatgaattg aattacattt taagcaaaca agcgtttttc gccttcgccg gctacgtttt
    21241 ccacacaacg tccgcaaact tcttcgtggc cagagtgcga gccgatgtcg gtagcataat
    21301 gccagcaacg agggcatttc tcaccatctg cattttccac ttttactgcg agtccggcta
    21361 attcgccttc cgcaatatct gcttccgcca gtggtttaac aatcgcttgt gaggtaatca
    21421 gcacaaagcg taattcattg ccaagttgtt ctaataacgg gcgaatttca tcattggcga
    21481 ataccgtcac cttggcttct aagccagcac caattacttt gtctttacgg gcttgttcca
    21541 gcacacggtt gacttccgca cgcactttta agatttgttg ccagtaagca tcatctaatt
    21601 tatcttgctc ggttaaaccg aataatccgg tgtagaattc ttcggtaaag acaaattcac
    21661 ttctgccttc cacctgcggt aagtagcccc aaatttcatc ggctgtgaag gataaaatcg
    21721 gtgcaatcca acgtactaac gcttctgcaa tatgccataa cgctgtttgg cagctacggc
    21781 gggcaaggct gtcggctttg gtggtgtatt ggcggtcttt gataatatct aagtagaaag
    21841 accccatttc aatcgagcag aaacgcatta aacgctgtac taccgtgtgg aattggtagt
    21901 tatcataggc ttctttgatg tcgttttgag cctctaaagc acaagccacc gcccagcggt
    21961 ctaaggcgat catctcttgc ggttgaacta aatcactttt tggatcgaaa ccgttcaagt
    22021 tcgccaataa gaaacgggct gtgttacgaa tacggcggta tgattccccg gcactgttta
    22081 aaatattgtg cgaaacggta atctcaccgg tatagtcggt ggaggcaacc cataagcgta
    22141 aaatatctgc accgtttttg ttccacactt cacttggcac aattacgttg ccaagcgatt
    22201 tggacatttt tctgcctttc tcatctacgg taaaaccgtg agttagcact tgtttgtatg
    22261 gtgctttgcc gttagtagca gtggaaagca ttaaagatga cataaaccaa ccacgatgtt
    22321 ggtctgaacc ttctaaatac atatccgctg aattgccgtt aaattcaggg cgttgttcta
    22381 caacagaggc ataggtcgat cctgaatcaa accacacgtc taaggtatcc ggcactttgc
    22441 ggtaatcttt ggcttcttca ccgagcaatt ccgcaggatc taaatcccac cacgcttgaa
    22501 tgcctttttc ttctacacgt ttcgccactt cttcaattaa ttctaaagtg cgaggatgta
    22561 attcttcggt ttcattatgc acaaacatcg ccattggcac gccccaagta cgttggcgtg
    22621 aaatacacca gtctgggcgg tttgccacca tcgtatcaat acgagcttca ccccagcttg
    22681 ggatccaacg caccgatttg atttctccta gtgcttgctg gcgtaagcct tgggtttcca
    22741 ttccgataaa ccattgtggg gtagcacgga agataatcgg ggttttgtgt ctccaacagt
    22801 gcggataact atgcttgatg cgggataatt ttaataatgc tcccacttct tgtaattttt
    22861 cgactacttt atcgttggat tcaaacacac ctaagccggc aaagaacggg gtgtctgctt
    22921 tgaatttacc gtcattggaa attagccctg ccatttcaat gttatatttg cgagcgacca
    22981 cgaagtcgtc cgcaccgtga tccggtgcag tatgcactaa ccctgtacca ccgtctgtgg
    23041 taacgtggtc gccaaaaata aatggcacgc tgtaatcata gaatgggtgg ttaaagcgta
    23101 ataattctaa gttttcgcct ttggtggttg ctaaaatctc agcagattca acgcctgctg
    23161 cttttgcaac atcttcgact aaatcgaccg ctagtaacac acgctcatca cctaattgga
    23221 ctaaggcata gtctaattca gggttgatcg caatcgcacg gttagacgga atagtccaag
    23281 gggtagttgt ccaaataacc gcagataatg cccctctacc tttaccctgt gcgttgaatt
    23341 tttcttctac cgcatttacg tccacagcat taaaacgcac ataaatagag ggagaaactt
    23401 tatcttcata ttccacttca gcttccgcta acgaagatcc acaatctaag caccaatgta
    23461 ccggttttga acctttgtat aaatgcccgt tcgcaatcac tttgccgagc gtgcgaataa
    23521 tattcgcctc agtgttaaaa ttcattgtta ggtatgggtt atcccaatca cctaaaatac
    23581 ccatacggat aaaatcggct ttctgacctt ctacttgttc tttcgcatag tcacggcacg
    23641 cttggcgaaa ctcggcagcc gaaattttct catttggttt accaacaatc ccttctactt
    23701 tcagctcaat cggcaaaccg tgacagtccc agcccggcac ataaggggta tcgaaaccta
    23761 atgcggtttt cgatttcaaa atcatatctt ttaaaatttt attcactgcg tgacctaagt
    23821 gaatattacc gttagcatac ggaggaccat catgcaaaat aaaggttttc ttgcctttcg
    23881 aggcagtacg aattttttgg tataaatttt tgtcatacca attttttaac atatcgggtt
    23941 cacgtttcgc gagatcccca cgcataggaa agcctgtttc aggcaagttt aaggtgtttt
    24001 tgtagtcaat agtcattttt ttatttctcg ttaaaattat ctgttttatg cctagcacgg
    24061 catagccgta ctaggttaag catagctcag cccctctggg gctggaaact agaaaggctc
    24121 accttgcaaa ttccttaaaa aattcaaccg cttgcaagcg atctttctca atctgggctt
    24181 ttagtgcttc aaagtcggga aattttattt cattgcgtaa tttttttaaa aagatcactt
    24241 caatcgcctc accgtaaata gaggcattga aatcaaaaat atgcacttct aataacggct
    24301 tagtaccgtt aatggtcggg cggttgccga cattggcgat tccgttaaaa ctgccctgtt
    24361 tggtcttcac ttttaccgca aatacccctt gaatcggcgt aaccaaacga ttgagcataa
    24421 tatttgcggt aggaaaacca atggttctgc cgagtttatt accgtgtgca acgcgtccca
    24481 gaatcgaata aggcttgccg agtaatttct ctgccaaatc taactcattg ttttgtaagg
    24541 cttctcgcac taatgagctg ctaattcttt cttcgtttaa gctatgggta tggctttctt
    24601 ccaccgcaaa gcgatatttt aagccggcat tgcgtaaagt ttcaaaactg cccgcccgtt
    24661 ttgccccaaa gcgaaaatca tcgcctacac ttaaatagcg tactttcagt cttttagcga
    24721 gcaaatcgct gatgaaatcg tctgccgaaa tttgagcaaa tttttcacta aaacgcacgc
    24781 atagcacaaa atccacaccg gcttcttcca aatattttag cttatctcgc aggcgcatta
    24841 agcgggcggg cgggttacaa gcggttgaat ttccactttt ttttgcaaaa aactctcgag
    24901 gttgtggctc aaataacatc acaactgagg gcaatcctaa atctaaagct ctatcacaaa
    24961 gacgagccaa aatattttga tgcccaagat gcacaccatc gaaattcccg atagagagaa
    25021 cacagccttg atttaaagcg atgtgtttgt ctaagttgtg gaaacctcga ataagttgca
    25081 taaaagataa accattttat aagtgaaaat tgctgtgatt ataacgagaa atctacaaaa
    25141 aaggaaaaaa attcaatctg ttatattttt tagtgctgaa agtggtcgtg tagc
//
LOCUS       DANLZC010000017        24956 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.017, whole genome shotgun sequence.
ACCESSION   DANLZC010000017 DANLZC010000000
VERSION     DANLZC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 24956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24956
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.017"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(91..654)
                     /locus_tag="PXE44_000548"
     CDS             complement(91..654)
                     /locus_tag="PXE44_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5358 domain-containing protein"
                     /protein_id="HDL5588089.1"
                     /translation="MLRKIVLLSTGLLLAACSSNEPQQPEAFPGEFANADYVLSEENA
                     QKWVIASEQAKQCIYPNLTRIQQEHFSKEDSYIFSQYVFFYPLEEIIGEQYVKIIQND
                     EKSMGYAQYLFKKLKNNEAVEALPKKQCATLRTNAKNDLAVVKGQYQSGMIEETNSQE
                     GKSGDKVATNDNKFFFDIIKWGSMLLL"
     gene            804..3401
                     /gene="mutS"
                     /locus_tag="PXE44_000549"
     CDS             804..3401
                     /gene="mutS"
                     /locus_tag="PXE44_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL5588090.1"
                     /translation="MTQDFSTHTPMMQQYLQLKAQNPDILLFYRMGDFYELFYDDAKR
                     AAALLDISLTKRGQSAGEPIPMAGVPYHAVEGYLAKLVALGESVAICEQIGDPATSKG
                     PVERKVVRIVTPGTVSDEALLPERQDNLVAAVYEEKGTFAIATLDMTSGRFLISELAS
                     KEALSAELQRVQPAEILYAEDFSAASILTNYKGLRRRPVWEFELVTAIQLLNRQFGTQ
                     TLAGFGVEKAVVALCAAGCVLHYAQETQRTALPHINAIHLAQHSDTILLDAATRRNLE
                     LTQNLAGGTENTLAAVLDKCVTPMGSRLLKRWIHQPIRDCGKLQARQKAITALIQYER
                     MSDLQPLLQQVGDMERILARVALRTARPRDLTRLRTALAQLPEIAKNSQNLTASIDQL
                     LAQLADFSPLHDLLERAIIENPPQLIRDGGVIAEGYNAELDEWRQLSDGATQYLENLE
                     IRERESTGIDSLKIGFNAVHGYYIQISQGQAHKAPIHYVRRQTLKNAERYIIPELKTY
                     EDKVLKAKGASLALEKQLYDELFDVLMPRLGELQLAAMMLSELDVLTNLAERADSLGY
                     VCPSFSPMRMVNIKGGRHPVVEQVLTEPFIANPVFLNSQRHLLIVTGPNMGGKSTYMR
                     QIALITLMAYIGSFVPAESAEIGPIDRIFTRIGASDDLASGRSTFMVEMTEMANILHQ
                     ATENSLVLIDEIGRGTSTYDGLSLAWACAEWLAKKTQSLTLFATHYFELTGLPNQLKG
                     VANVHLDAREHQDSIVFMHSVQEGAASKSYGLAVAALAGVPKQVIQLAKQRLAHLEEI
                     SHQTKQANEHPQADLLFSDGFDLQKNEAIQPLVVENEVEKALKALQPDELTPRQALDE
                     LYRLKKLLG"
     gene            complement(3449..4903)
                     /locus_tag="PXE44_000550"
     CDS             complement(3449..4903)
                     /locus_tag="PXE44_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5588091.1"
                     /translation="MKKIVSTLLILTSNLAFAETTPAQLQEKMDNQRMQKNLHTLEKP
                     EPQAKPQAVNAQAHRQSISISSQQLLQQPELLNSAMLTALVTNHTENVQFLLPIYQQL
                     PASKQNSEVVQWAEAVNAKAKQNYSEAIKGYRQLLAKYPENQPIRMQLAVALFENREN
                     EAAEAQFYKLQADKLPQEIANAVNSYLEAIGKQDQWSFQGGLTYLNDPNINNAPNAGT
                     TYGNWTAPKKESAQGVGFHFEADKKWSWGNGFFHEFRLNGNGKYYWNNKKYNEYSLRE
                     SLGLGYQNAKQKITLLPFFEQMWYAGGSSQTESTKRYSNAGGVNARWQYWITPQWQSA
                     ISYEYAEQRYTRRQHLDGNYHFIAPSLYFYPNSRQYWFVGANFNRTSPRDLDDSFIRR
                     GVSIGWGQEWKNGLSTQLSASYARKQYRAPMPIFQITQRNSEYGVQASIWHRALHFKG
                     ITPRLTWQYNKVKSNHSFYSYDKNRVYIEFSHTF"
     gene            complement(5127..5765)
                     /locus_tag="PXE44_000551"
     CDS             complement(5127..5765)
                     /locus_tag="PXE44_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL5588092.1"
                     /translation="MRYTTEQIIEKLRQLKVVPVIALDKAEDILPLAKTLSENGLPVV
                     EITFRSEAAEEAIKLVQANYPEVLIAAGTVLTKEQVLTAKNAGADCIVTPGFNPTIVK
                     YCQVLGLPVTPGVNNPMAIEAALELGIQAVKFFPAEASGGVKMIKALLGPYGNLQIMP
                     TGGISPQNINDYLAIPNVVACGGSWFVEKSLIKEQKWEEIGKLTKEVVELVK"
     gene            complement(5853..6800)
                     /locus_tag="PXE44_000552"
     CDS             complement(5853..6800)
                     /locus_tag="PXE44_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL5588093.1"
                     /translation="MKKLAILGECMIELNGEPFGEMHQTYGGDSLNTATYLARVSSPE
                     QIEVRYISALGTDKLSQGMLAAWQVDNINTDFVLRDEHRQPGLYLIQLDKQGERTFLY
                     WRNQSAARYLLQHPGYPKALSALNEVDMVYLSGISLAILPENDRTLLIAQLRELKTQG
                     VKIAFDSNYRPKLWESVAAAQAHYAELLQLVDLALVTFDDEQLLWGDVNEQATFDRLT
                     QYGIQTIVVKQGSLGATVLQNGERTFVPTVAVEKVVDTTSAGDSFNAGFLNGYLLGKD
                     VVTCCQQGNAVAGIVIQHKGAIIAKSATTHLKSQFEQSI"
     gene            complement(6817..7662)
                     /locus_tag="PXE44_000553"
     CDS             complement(6817..7662)
                     /locus_tag="PXE44_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5588094.1"
                     /translation="MSIAQNHNFKDKVVVITGAGGVLCAYFAKEIAKTGAKVALLDIN
                     LEAAQNFASEIADEGYIAKAYQTNVLELESIKQARDEILKDFGTCDILINGAGGNNPK
                     ATTDNEFHDINLPENTKSFFELDKAGIEFVFNLNYLGTLLPTQVFAKDMLGKEGANII
                     NISSMNAFTPLTKIPAYSGAKSAISNFTQWLAVYFSKVGIRCNAIAPGFLVSNQNKAL
                     LFNADGTPTARANKILTNTPMGRFGQADELVGAILFLMDHHYSSFINGVVLPVDGGFS
                     AYSGV"
     gene            complement(7674..8945)
                     /locus_tag="PXE44_000554"
     CDS             complement(7674..8945)
                     /locus_tag="PXE44_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL5588095.1"
                     /translation="MLWLFLGSLFLFLFLGVPVALSMLLSASFMLWKMDMFDAQLITE
                     NFVMGTNNFPLMAIPFFMLTGEIMKHGGISERIINFAMSMVGHIKGGLGYVVIISGLI
                     FAGLSGSAVADTAALGAILIPMMVARKYDVARSTGLICAAGIISVVIPPSIPMIVYGI
                     TAGASITKLFMGGMVPGFLMVLGLWLVWKFLYRNEEQTVEPKKTGKERWAAFKSAFLP
                     LLLPIIIIVGLRGGIFTPTEAGVVAAIYAAIISIILKGLTLDKIQDVFLGTIKTTAMV
                     MFVAAAAMISAFAVTVAQIPMELVEFIKGITDSPTVLMFVIMIFLLLVGCVMDLIPAV
                     LIFVPVLLPLLRAYNIDIAYFGIMMVINLSIGLITPPVGTVLYVGSGIAKIGIARLTK
                     GIYPFLLVYAAILFLLILFPEIIIVPMNWLS"
     gene            complement(8948..9427)
                     /locus_tag="PXE44_000555"
     CDS             complement(8948..9427)
                     /locus_tag="PXE44_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL5588096.1"
                     /translation="MQISTALDRTLKLLAIIALSTMIILVFFNAVLRYFFDSGIAWSE
                     EFARICFVYMIFLGIILVAKERGHLTVDIVVSALPAKVQMYLLFFADILVLVALSFIT
                     YGAYQLMLLTYTQKMPATEISSAFLYFAAVISSISYFAIVFTNLWKHIRLAFIKEEK"
     gene            complement(9488..10471)
                     /locus_tag="PXE44_000556"
     CDS             complement(9488..10471)
                     /locus_tag="PXE44_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5588097.1"
                     /translation="MKLKTFTKALLISSLMFTGSAFAKTIIKIGHYNSDVHPSNIALQ
                     EYFKKTIEEKTEGRYEIRIFPNNQLGGEDQIVNGLRNGTIEGGITGLLLQNVDPIFGV
                     WEWPYLFKDNAEAKKVLESPIAQEIGEKMEKYGIKLLAYGMNGFRVISSNKKVENFDG
                     FKGLRLRVPLNSLFVDWAKAMHINPQSMPLSEVFTALEQKVIDGQENPYMLIKDSGLY
                     EVQKYIIESNHIFSPGLLQLSLKTWNKMSKEDQTIFLEAAKLYQEKEWELAMKMEQDV
                     KDFFHKNGKEVIVPSEQFKADMLKASETLYQNFYQKYDWAKDVISKIHAAK"
     gene            complement(10500..11525)
                     /locus_tag="PXE44_000557"
     CDS             complement(10500..11525)
                     /locus_tag="PXE44_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL5588098.1"
                     /translation="MEKPLKSVCLVEPNKVGIQTVSYPVKGENDVLIKVEGMGICGSD
                     IGAFRGTNPLVTYPRILGHEIVGTVIESGKGMPNNINVGDRVIIEPYIYCGHCYPCSI
                     GRTNCCESLKVLGVHIDGAMQEVVRHPAHMIIKTPDMPIHELALAEPLTISLHAIHRT
                     KVKAGEHVAIIGAGAIGLMAALVAKAYGATPILIDILDKRLDYAKSIGIPHTINPTKE
                     NDLEAIKSITNGRMAEVVIEASGANVAVQNTLKYTSFAGRIALTGWPKTETPLPTNLI
                     TFKELNIYGARTSKGAFEEALELLASRRIEPKDIISKVITFDEIPHYIEELSANPDDY
                     LKIIAVF"
     gene            11689..12450
                     /locus_tag="PXE44_000558"
     CDS             11689..12450
                     /locus_tag="PXE44_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HDL5588099.1"
                     /translation="MQNSSRTYTKIGKILKEEIAQGKYRVGDKLPTERDISERFEVSR
                     TIVREAIVMLEVEKLIEVKKGSGVYVIRVPELFPEDFPEEKDNELRDVGPFELLQARQ
                     LLESSIAEFAAQQATKQDIQVLRNILESEKKLLLEKMDDYSADKEFHLALAHMTGNDV
                     LIQLQEQLWRYRFNSAMWAQLHSRILQHDHHHLWIEDHERILNAIQRKNPALARKAMW
                     QHLENVKTKLLELSDVEDPKFDGYLFNVNPVVVGI"
     gene            12480..13664
                     /gene="uxuA"
                     /locus_tag="PXE44_000559"
     CDS             12480..13664
                     /gene="uxuA"
                     /locus_tag="PXE44_000559"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL5588100.1"
                     /translation="MEQAWRWYGPNDPVSLDDVRQAGATGIVTALHHIPNGQVWSVEE
                     INKRKALLAEKGLVWSVVESVPVHEEIKTQTGNYQQWIDNYKQTLRNLASCGIDTVCY
                     NFMPVLDWTRTDLEYEMPDGSKALRFDQVAFAAFELYILKRPGAEATYSSAEQAEAKA
                     YFEQMSDADIAKLTKNIIAGLPGSEEGYTLEEFQAQLDRYQGLSAEKFRTHLAYFLNE
                     IVPVAQEVGIRMAIHPDDPPRPILGLPRIVSTIEDMQWFVETQPLAANGFTFCTGSYG
                     VLAENDLVKMAEQFADRIYFAHLRATQREENPQSFHEADHLAGDVDMFGVVKALLTEE
                     YRRKANGDHRLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLRGLAEFRGLELALKKVYF
                     ND"
     gene            13715..14584
                     /locus_tag="PXE44_000560"
     CDS             13715..14584
                     /locus_tag="PXE44_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5588101.1"
                     /translation="MFKKYFGEIILFLVTFIAAAGWFFSKHALVGLPPIGFMGGRFIL
                     ASLILLPFSYRELYRLDKVQVKYSVITGLTYGIYTIFWVLGLAFSTVFGEGAFLVSLA
                     MLIAPLLSWLIFKHTPPKIFWLALPIALSGLYFLSAEKGIFHFSLGSTIFLVSSVFGA
                     LYFVLNNQYAKQVSLFPLITIQLAIVGAVCSLYSYFMESWHFPILTEVWLWFAASVLI
                     ATNLRFLMQTLGQKYCHIGTSALIMLLEPVWTLLLSVMLLGEQLSATKWLGCSLILIA
                     LIIYRLPILRLKK"
     gene            14752..15066
                     /gene="rsfS"
                     /locus_tag="PXE44_000561"
     CDS             14752..15066
                     /gene="rsfS"
                     /locus_tag="PXE44_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL5588102.1"
                     /translation="MEQNLVEFLTKTLDDLKAQDILAIDVKGKSSITDTMILATGTSS
                     RHVASTAERLITEAKQAGFEVFGDEGKAVADWIVVDFGKAMVHLLQSDSREMYQLEKL
                     WA"
     gene            15143..15610
                     /gene="rlmH"
                     /locus_tag="PXE44_000562"
     CDS             15143..15610
                     /gene="rlmH"
                     /locus_tag="PXE44_000562"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL5588103.1"
                     /translation="MKIQLIAVGTKMPAWVTTGFEEYQRRFPKEMPFELVEIPAGKRG
                     KNADTKRILELEGKAMLAAAGKAKIVTLDIPGKAWTTEQLATQLEGWKNDGRDVALLI
                     GGPEGLSPECKAAAEQSWSLSPLTLPHPLVRVVVAESLYRAWSVTINHPYHRE"
     gene            15634..17583
                     /gene="mrdA"
                     /locus_tag="PXE44_000563"
     CDS             15634..17583
                     /gene="mrdA"
                     /locus_tag="PXE44_000563"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL5588104.1"
                     /translation="MFGKLAKFTASNQREKVRDGQAESNLYARRALVAFLGVLALSGV
                     LLTNLYHLQVVNYEDYQTRSNGNRIKLLPVPPARGLIYDRNGKVLAENLTYFGLFIVP
                     EKTENIEQTLTELKDIVGLTDEDIENYHKEKKRSSRYTPIMLKGNLNEAQMARFAVNQ
                     YRFPSLDVQAYYKRNYPYGELLTHIIGYVAKINENDKKKLQEAGKFGNYAGSHDMGKL
                     GIEKFYEDQLHGVTGFEEVEINNRGKVIRKLRDQAGVAGSSIRLSIDVELQQYVMSLI
                     AKHKGAVVVLDPRDSSILAMVSNPSYDNNLFVGGISGQDYKALLDDPTRPLYSRTTQG
                     TYPPASTIKPFMAVAGLEEGMITPSTTISDPGYWILPGTTQRYRDWKRSGHGATNVNK
                     AITESSDTFFYQLAYKMGIDKMSDWMKKFGFGVKTGIDIFEESAGVMPTREWKQKRYK
                     KPWVQGDTIPVGIGQGYWISTPLQLAKATAVLINNGRVNTPHLMLEVQSSNSVTPYQD
                     PLLYEDIKGVPDVYWNLAKQGMYNVNHAANGTARKVFAGAFYHSAGKTGTAQVFNLNG
                     KNYDKNAIKKELHDHAWFIGYAPYEQPKIVVALILENAGGGGSAAAPVARQIMDYALR
                     KGIGEQPDSQNSEQNKPLVTEEHHE"
     gene            17576..18703
                     /gene="rodA"
                     /locus_tag="PXE44_000564"
     CDS             17576..18703
                     /gene="rodA"
                     /locus_tag="PXE44_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDL5588105.1"
                     /translation="MNSGFKKLFWKIFSLDLLLLSGLLAITGYGLLVLYSASGANERM
                     FTSRLIQVSLGLGLMLIMAMVPPRFYQKVSPYLYIFCLILLILVDLVGETSKGAQRWL
                     NLGFVRFQPSEIAKLAVPLMVATYLGKRPLPPSFKDTFIALAIIIVPTLLVAIQPDLG
                     TSILVCSAGIFVLFLAGLSWKLIGAGVVFLAGFIPIMWFFLMHDYQKTRVMTLIDPNK
                     DPLGAGYHIIQSKIAIGSGGINGKGWMEGTQSQLEFLPEPHTDFIFAVLSEEHGMIGV
                     LILLAIYLFIIARGLVIGTKSDSAFGRILSGGTALLFFVYVFVNIGMVSGILPVVGVP
                     LPLFSYGGTSYVTLMAAFGLMMSTYVHRKRVGSNNPYDKLK"
     gene            18795..19454
                     /locus_tag="PXE44_000565"
     CDS             18795..19454
                     /locus_tag="PXE44_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HDL5588106.1"
                     /translation="MRLGKIVTLLLAGFLTFSSLNSLAATKNTKAKSSILAKKQATSK
                     AIKKVGISKVKKKEVVAKATKKKTISTVTTKPIKSTKKLARKVVKKTVTAKKKLVKKQ
                     HKPLVKKHFQTGVASYYANYFNGRRTANGETFNNAKMTAAHRTLPFGTLIEVTNLRNG
                     RSVVVRVNDRGPYAHARVLDLSSAAAKKIGMHHSGTARVKIAIVDKSKLGSTNQFYVM
                     N"
     gene            19468..20646
                     /locus_tag="PXE44_000566"
     CDS             19468..20646
                     /locus_tag="PXE44_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL5588107.1"
                     /translation="MKKTLVKAMSVVGFAVSSIAYADLNTNFNLSAPQINAQTYILMD
                     YNSGEVLVSLNPDQRQYPASLTKMMTSYVVGEALKSGKVQNSDMVIVTENSWAQKFPG
                     SSLMFLDLNSKVSVGELMKGLIIVSGNDASVALAEHVSGSQAAFINEMNKYAQQFGLK
                     NTHFTTVHGLDDPEQYSSARDMAIIGSHIIRDQPEEYKIYAEKEFQYNIKKPQPNRNG
                     LLWDKTIQVDGMKTGHTDKAGYNLVASAINPNTRLISVVMGVPTYKGREVESKKLLQW
                     GFANFETIKSIPAAQSVAEQSVYYGEVNKVQLGSVQDSFVTIPKGRSADLKARYELEK
                     KNLEAPLAKGQVVGKMIYQLDGKDVASTNLQVLQDVPEAGIFGKAWDWIALTVKSLFD
                     "
     gene            20814..21113
                     /locus_tag="PXE44_000567"
     CDS             20814..21113
                     /locus_tag="PXE44_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDL5588108.1"
                     /translation="MSQPKAINLQDLPQAKLKDLLEFPCHFTFKVVGANREDLVDDVV
                     VVTQKYAKGDYNPRQQKSSKGTYNSVSIDIVAENIEQVETLYTELAKINGVRMVL"
     gene            21175..21828
                     /gene="lipB"
                     /locus_tag="PXE44_000568"
     CDS             21175..21828
                     /gene="lipB"
                     /locus_tag="PXE44_000568"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDL5588109.1"
                     /translation="MKLIIRQLGIQPYEEIWHQMQSFTDSRDGNTDDEIWLVQHPAVF
                     TQGSAGKPEHLLNPTNIPVVQSDRGGQITYHGEGQQIMYVLIDIKRLKSLGKEVSVRD
                     LVTALEQTVVQTLAEYGIESYPKADAPGVYVDGKKICSLGLRIRKGCSFHGLALNINM
                     DLTPFQNINPCGYAGLEMCQLSELVHRPDLRCEEVAPKLVAHFSTILGYNNQQIINN"
     gene            21843..22850
                     /gene="lipA"
                     /locus_tag="PXE44_000569"
     CDS             21843..22850
                     /gene="lipA"
                     /locus_tag="PXE44_000569"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDL5588110.1"
                     /translation="MATSTAQTTATATVKKMSPFKMEKGVKYRDAAKTSVIQVRNIDP
                     DQELLKKPEWMKIKLPANSAKIDSIKNGMRRHGLHSVCEEASCPNLHECFNHGTATFM
                     IMGAICTRRCPFCDVAHGKPLPLDPDEPRKVAETVQDMKLKYVVITSVDRDDLPDRGA
                     AHFSATVREIKALNPHCKVEILVPDFRGRVEQAVAILKENPPDVFNHNLENVPRLYRE
                     VRPGADYKWSLELLKIFKQEFPHIPTKSGLMVGLGETNEEILEVMQDLRDHGVTMLTI
                     GQYLQPSRHHLKVERYVPPAEFDMFREEAEKMGFEHAACGPFVRSSYHADLQAKGELV
                     K"
     gene            23037..24140
                     /locus_tag="PXE44_000570"
     CDS             23037..24140
                     /locus_tag="PXE44_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5588111.1"
                     /translation="MKKLLLNSVFAATLLAFGATANAEIKTITDVLGREVKVDVPAKR
                     VALTFYYPDYIAVTGVENFDKVVGISREFWEKFNPGSWALFAEKMPNLKNIADLGYVN
                     SGTFSTEKTIALKPDVLVLPEIQYQALASEISRIEQAGIPVVVDFNKQTIENHTKSVR
                     VFGQLTGTEERAEKIAKEYEEGIADIQSCIDNAKVSKPKIYAEFGNKGPKEHSFTFGK
                     NMWGAIAETVRGDNISAPFVENWGQINPEQVLVSKPDVIMISGTELGNDTNPEIMSMG
                     INIEEADAQKRLKGFIERTGWKELPAVKSGRVYGLYHTASRSISDLASAQFMAKALYP
                     ELFKDIDPEKTYLDFHNNYLPITPKGTFFMQLK"
     gene            complement(24231..>24956)
                     /locus_tag="PXE44_000571"
     CDS             complement(24231..>24956)
                     /locus_tag="PXE44_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5588112.1"
                     /translation="LVVADKINLHFEQGKIYTLLGPNGAGKSSLLKTLFGEIAHQGTI
                     DFDGNKLTSSHLHSWRKEIGYMPQDTQVEASLSALEVILLGQLDSLNMYISDELLNEA
                     VDIMDKLGISHLAHQDIMRLSGGQRQMVMFAQVLLRKPHILLLDEPVSALDMYHQINL
                     LEYVNAYTRANNLITIMVLHDLSLAAQFSDSVMLLSEGKFKHKAELVICWNAILFRDS
                     IMSILRFYMMETARRLSARFANV"
BASE COUNT         7268 a         4896 c         5521 g         7271 t
ORIGIN      
        1 tgtattttaa gactgaggct ttagataaaa gttcaatcgg taggtataaa tggctcggtt
       61 gtggttaaaa caaccgagta ttgaggctca ctagagaagt aacatactgc cccatttgat
      121 aatatcgaag aagaatttat tatcattggt ggcaacttta tcgcctgatt tcccttcttg
      181 gctattggtt tcttcgatca taccgctttg atactgccct tttaccactg ccaaatcatt
      241 ttttgcattg gtgcgtaagg tcgcacattg ttttttcggt aacgcttcaa ccgcttcgtt
      301 atttttcagt tttttgaata aatattgagc gtaacccatt gatttttcat cattttgaat
      361 gatttttaca tactgctccc cgataatctc ttcaagcgga tagaaaaata catattgtga
      421 gaaaatataa gaatcttctt tggaaaaatg ctcttgctga atacgggtta aattggggta
      481 gatacactgt tttgcctgtt cgctggcaat tacccatttt tgagcatttt cttccgaaag
      541 cacataatcg gcattggcaa attcgcccgg gaatgcttcc ggttgttgcg gttcattgct
      601 gctacaagcc gccaataata agcctgtaga aagtagcact atttttctta acattctgac
      661 ctctgtttta aaaatttggt gcattgtaac aaaaaaacag cggctgacca ttgcaaattt
      721 tcagcgaaaa atgaccgctt gtttttatcc cttggctgaa tctgttaaac tatcagcaat
      781 gatttctcac aataaataga ctgatgacac aagatttctc cacccatacg ccaatgatgc
      841 aacaatattt gcaactcaaa gctcaaaatc cggacatact attgttttac cgaatggggg
      901 atttttatga gctgttttat gatgatgcga aacgagcggc agcattgttg gatatttcgc
      961 tcaccaagcg agggcaatct gccggtgagc cgattccgat ggcgggtgtg ccttatcacg
     1021 cagtggaggg gtatttggcg aaattggtgg cgttggggga atcggtagca atttgtgaac
     1081 agattggcga cccagcaacc tcaaaagggc cggtagaacg caaagtggta cggattgtta
     1141 cgccgggaac agttagcgat gaggcattgt tacccgaacg ccaagataac ttggtggcgg
     1201 cagtttacga agaaaaaggc acatttgcga ttgctacgct agatatgacc tccgggcggt
     1261 ttttaatcag tgaacttgct agcaaagagg ctctatccgc cgagttacaa cgtgtacaac
     1321 cggcagaaat tctgtatgcg gaagattttt ccgctgcctc aattttaacg aattacaaag
     1381 ggttacgccg ccgtccggtg tgggaatttg agctggtaac ggcgattcag ctgctaaatc
     1441 gccaattcgg cacgcaaaca cttgccggat ttggggtgga aaaagcggtg gtggcacttt
     1501 gtgctgccgg ctgtgtcttg cattatgccc aagaaacgca acgcaccgct ttaccgcaca
     1561 ttaacgcaat tcatttggca caacatagcg atactatttt gctcgatgca gctactcgtc
     1621 gcaatttaga gctgacccaa aacttagcgg gtggcacgga aaacacgctg gcggcggtgt
     1681 tagataaatg tgtgacccca atggggagcc gtctgctcaa gcgttggatt catcagccga
     1741 ttcgagattg tggtaaatta caagcccgac aaaaggcgat aacagcattg atacaatatg
     1801 agcgaatgtc ggatttacag ccgttattac aacaagtcgg cgatatggag cggattctcg
     1861 cccgagtggc gttgcgtacg gctcgccctc gtgacctcac ccgactacgc actgcattgg
     1921 ctcagttacc ggaaattgca aaaaattcac aaaatctgac cgctagtatc gatcagcttt
     1981 tggctcaact ggcggatttc agcccgttac acgatttgct ggagcgggcg attatcgaaa
     2041 acccaccaca actcattcgt gatggtgggg tgattgcaga aggttataat gccgagcttg
     2101 acgaatggcg gcaactttcg gacggggcaa cgcaatatct ggaaaatttg gagatcagag
     2161 aacgtgaaag caccggcatt gattcgctga aaatcggctt taatgcggtt cacggctatt
     2221 acattcagat cagccaaggg caagcacaca aagccccgat tcactatgtt cgccgccaaa
     2281 cgctcaaaaa tgcggagcgg tatattatcc ctgagctgaa aacttacgaa gacaaagtgc
     2341 tgaaagcaaa aggggcatcg cttgcccttg aaaaacagct ttatgatgaa ctgtttgatg
     2401 tgttaatgcc tcgcttaggc gaattgcaat tagcggcgat gatgctgtct gaacttgatg
     2461 tcttaaccaa ccttgccgag cgagctgata gtttaggcta tgtttgcccg agctttagcc
     2521 cgatgcgaat ggtgaatatt aaaggcggca gacacccggt ggtggagcag gttttaaccg
     2581 agccgtttat tgccaatccg gtgtttttaa attcccaacg gcatttattg attgtgaccg
     2641 gcccgaatat gggcggtaaa agcacctata tgcggcaaat tgccctcatt acattgatgg
     2701 cgtatatcgg cagttttgtg ccggcagaaa gtgcggaaat cggcccgatt gaccgtattt
     2761 tcacccgaat tggggcaagc gatgatttag cttcagggcg ttcaaccttt atggtggaaa
     2821 tgaccgaaat ggcgaatatt ctgcatcaag ccaccgaaaa cagccttgtg ctgattgatg
     2881 aaatcgggcg aggcacttct acctatgacg gcttatcact ggcgtgggcg tgtgccgaat
     2941 ggctggcgaa gaaaacccag tcactcactt tgtttgcaac ccactatttt gaacttaccg
     3001 gcttgccgaa tcagctcaaa ggggtggcaa atgtgcattt agacgcgcgt gaacatcaag
     3061 acagtattgt gtttatgcac tcggtgcaag agggggccgc cagcaaaagt tacggcttgg
     3121 cggtggcagc cttggcgggt gtgccaaagc aagtgattca actggcaaag cagcgtttgg
     3181 cacatttgga ggaaatttcc caccaaacca aacaagcaaa cgaacatcca caggcggatt
     3241 tgttgttttc cgatggtttt gatttgcaaa aaaatgaagc aattcaaccg cttgtagtag
     3301 aaaatgaagt tgaaaaagcc ctgaaagccc ttcagcctga tgaacttacc ccaagacagg
     3361 ctttggacga gttgtatcgc ttgaaaaaat tgttggggta aattgaaaaa ataactaaga
     3421 aatcgccccg tttaaggggc gtttttgttt agaacgtatg tgaaaactca atatagacac
     3481 ggtttttgtc ataactatag aagctatgat tgctttttac cttgttatat tgccacgtta
     3541 agcgaggggt aataccctta aaatgcaagg ctctgtgcca gatgctggct tgcacaccat
     3601 attcactgtt acgttgtgta atttggaaaa tcggcattgg ggctcggtat tgtttgcggg
     3661 cgtagctggc acttaattgg gttgaaagcc cgtttttcca ctcttgtccc caaccaatag
     3721 aaacacctcg gcgaataaag ctatcatcta aatcacgggg gctggtgcgg ttaaaattcg
     3781 cccccacaaa ccaatattgc cggctgttag ggtaaaaata gaggctcggg gcaataaaat
     3841 gataattgcc gtctaaatgt tggcggcgag tgtaacgctg ttccgcatat tcgtagctga
     3901 ttgcagattg ccactgtgga gtaatccaat attgccagcg ggcattcaca ccgcctgcgt
     3961 tggagtagcg tttggtgctt tcggtttggc ttgagccacc ggcataccac atctgctcaa
     4021 aaaacggcaa caaggtgatt ttttgttttg cattttgata acccaagccg aggctttctc
     4081 tgagcgaata ttcgttatat tttttgttat tccagtaata tttgccgttt ccgtttaaac
     4141 gaaactcgtg gaagaaacca ttgccccaag accacttttt atccgcctca aagtgaaagc
     4201 cgaccccttg ggcggattct tttttcggtg cagtccagtt gccataggtc gtgcctgcat
     4261 ttggagcatt gttgatattc gggtcattca aataggttaa gccgccttga aaactccatt
     4321 gatcttgctt gccaatcgcc tccaaatagc tattgaccgc atttgcaatt tcttgcggta
     4381 atttgtccgc ttgtaactta taaaattgag cctctgcggc ttcattttcc cgattttcaa
     4441 acaatgcaac cgctaattgc atacggattg gctgattttc agggtatttt gccagtagct
     4501 gacggtagcc ttttattgcc tctgaataat tttgttttgc tttggcatta actgcctccg
     4561 cccattgtac aacttcgctg ttttgtttac tggcgggtaa ttgttgataa ataggcagta
     4621 agaattgaac attctcagtg tggttagtga ccaacgcagt tagcattgcc gagttgagca
     4681 actcaggctg ttgaagcagc tgttggcttg aaatagagat cgattgccgg tgagcctgtg
     4741 cattaaccgc ttgtggtttg gcttgaggtt caggcttttc taatgtatgt aggtttttct
     4801 gcattcgctg gttatccatt ttttcttgaa gttgagccgg tgtggtttcc gcaaatgcca
     4861 ggtttgaggt aaggattaaa agcgttgaga ctattttttt cataaagcta tcttttattt
     4921 gaatagaaaa ggctattttt caatagcctt tagtgaagag ttttgaggtt gttttgctat
     4981 atttttcatt aagttaaata tatagcttta aatataccta ctatatgcta ctaaataaag
     5041 tatgcaatat ttgttatgct ttgtaaataa gaggaaagag atccattttg ggtagaaaat
     5101 agctcattgc ttggaacttt aaatggttat ttcaccaatt caaccacttc cttcgtcaat
     5161 ttcccaatct cctcccactt ctgttcctta atcagggatt tctccacaaa ccaagagccg
     5221 ccgcaggcga ctacgttggg aatggcgagg tagtcgttga tgttttgtgg cgagatgccg
     5281 ccggtcggca taatctgtaa gttgccgtaa gggccgagca gggctttaat cattttcacg
     5341 ccgccactgg cttcggcagg gaaaaattta actgcttgta tgccaagttc gagggcggct
     5401 tcaatcgcca tcgggttgtt cacacccggt gtaacaggca aacctaacac ttggcaatac
     5461 ttaacgatag tcggattaaa acccggagtc acaatgcaat ccgccccagc gtttttggca
     5521 gttaagactt gctctttggt cagcactgtg cctgctgcaa tcaatacttc cggatagttg
     5581 gcttgcacta atttaattgc ctcttcagct gcttccgagc ggaaagtgat ttccaccacc
     5641 ggcaaaccgt tttcggaaag ggttttcgcc agcggtaaaa tatcttctgc tttatccaaa
     5701 gcaatcacgg gaacaacttt cagttgtctt aatttttcaa tgatttgttc ggtggtgtaa
     5761 cgcatttcta gcctcattat gttgtattgc aagcggtcgc ttttgtaaaa aattttgcaa
     5821 atttaaccgc ttgtcgttgt gctattgttg gtctaaatgg attgttcaaa ctgcgatttc
     5881 aaatgagtag tggcggattt ggcaataatc gcccctttgt gctgaatgac gataccggca
     5941 acggcatttc cttgttggca acaggtaacg acatctttac ccaataagta accgttaagg
     6001 aagccggcat taaaggaatc gccggcggaa gtggtatcga ccaccttctc taccgcaaca
     6061 gttggcacaa aagtacgctc cccgttttgc aacaccgttg cccccagaga gccttgtttc
     6121 accacaatgg tctgaatgcc atattgagtg agacggtcaa aggtggcttg ctcattcaca
     6181 tcgccccaaa gcagttgctc gtcatcaaag gtcacgagag ctaaatcgac taattgcaac
     6241 agctcggcat agtgagcttg ggcagcagcc acactttccc acagtttcgg ccggtaattg
     6301 ctgtcaaagg cgattttcac cccttgtgtt ttgagctcac gcaactgtgc aatcagcaaa
     6361 gtgcggtcgt tttccggcaa gatggcaagg ctgataccgc tcaagtaaac catatctacc
     6421 tcgtttagtg ccgagagtgc tttgggatag ccggggtgtt gcaacaaata acgggcggca
     6481 gattgatttc gccaatagag gaacgttcgc tcgccctgtt tatccaactg aatcagatac
     6541 agccccggct ggcggtgctc gtcacgcaac acaaaatccg tgttgatatt atccacctgc
     6601 cacgccgcta acattccctg actgagctta tccgtgccga gagcggaaat ataacgcacc
     6661 tcaatctgtt caggcgaact cacccgagcc aaataggtag cggtgttgag cgaatcccca
     6721 ccataggttt ggtgcatctc cccaaacggc tccccgttta actcaatcat acattcaccg
     6781 agaatggcga gttttttcat aggaatacct tctaagttac acgccgctat aagcagagaa
     6841 tccgccatca acaggtaata caacgccatt gataaagctg gaataatggt gatccattaa
     6901 aaataggatt gcacctacca attcatcggc ttgtccgaaa cgtcccatag gggtattcgt
     6961 gaggatttta ttagctctag ctgttggcgt accatcggcg ttaaatagta gagctttatt
     7021 ttgattactc actaaaaatc ccggtgcaat agcattacag cggataccta cttttgagaa
     7081 ataaactgct aaccattgtg taaagttact gattgcagat tttgctccag aataggcagg
     7141 gattttagtt aatggagtaa aggcattcat actggatata ttaatgatat tagcgccctc
     7201 tttacccaac atatcttttg cgaaaacctg tgttggtaat agtgtgccta agtaatttaa
     7261 attaaataca aattcaatcc ctgctttatc taattcaaaa aaagattttg tattttccgg
     7321 taagttaata tcgtgaaatt cattatctgt cgttgctttt ggattattcc cacctgcacc
     7381 attaattaaa atatcacaag tgccaaaatc ttttagaatt tcatctcttg cctgttttat
     7441 gctttctaat tcaagtacat tagtttggta cgctttcgca atatagcctt catcggcaat
     7501 ttcagaagca aaattctgtg ctgcttctag attgatatcg agtagtgcaa cttttgcacc
     7561 tgtttttgca atttcttttg caaaataagc acatagcaca ccacctgctc cggtaataac
     7621 aacaacttta tctttaaaat tatgattttg ggcaatagac ataataagaa ctcctaagat
     7681 aaccaattca ttggtacgat aataatttca gggaaaagta ttaataagaa cagtattgca
     7741 gcatagacta gcaagaatgg atagatacct ttggttaatc tagcaatacc aatttttgct
     7801 ataccagaac caacatataa taccgttcca acaggaggcg taattaaacc gatagatagg
     7861 ttaattacca tcataatacc aaagtaagca atatcgatgt tatacgctct tagtaatggt
     7921 aatagtactg gtacaaaaat taatactgct gggattaagt ccattacaca gccgactaat
     7981 aataagaaaa tcataatgac gaacattagt acagtcgggc tatcggtaat acctttgata
     8041 aactcaacta actccatagg tatttgagca acagtaactg caaatgcaga tatcattgcc
     8101 gccgctgcaa caaacataac cattgcagtt gttttgatag tgcctaagaa aacatcttgg
     8161 attttatcta aggttaaccc ttttaagata atgctgatta ttgctgcata aattgctgct
     8221 acaactcccg cttctgttgg tgtaaagatg ccgcctctta aacctacgat aataataatt
     8281 ggaagtagca atggtaagaa tgctgattta aatgcagccc aacgttcttt acctgttttt
     8341 ttcggttcaa cggtttgttc ttcgtttcgg tataagaatt tccaaactaa ccataaacct
     8401 aaaaccatta aaaagcctgg aaccattcct cccataaata atttggtaat tgatgcaccg
     8461 gctgtaatac catagacaat cattggaatg cttggtggga taactacaga aataatacct
     8521 gctgcacaga ttaagcctgt tgaacgtgca acatcatatt ttcttgcaac catcatcgga
     8581 ataagaattg caccgagagc cgcggtatca gcaacagcgg agccggataa acctgcgaaa
     8641 attaagcctg aaataatcac tacatagccc aatccgcctt taatatggcc gaccatgctc
     8701 attgcaaaat tgataatacg ttcggaaata ccgccgtgtt tcataatttc accggtcagc
     8761 ataaaaaacg ggatagccat cagcggaaaa ttatttgtac ccattacgaa attctccgtg
     8821 attaattggg catcaaacat atccattttc catagcataa aactggcact aagtagcata
     8881 gagagagcaa cgggcactcc taaaaagaga aagaggaata gacttcctaa aaatagccat
     8941 aacatattta tttctcctct ttaatgaaag ctagccgaat atgtttccat aaatttgtga
     9001 atacaatagc gaaataggag atagatgaaa taactgctgc aaaatataga aacgcagatg
     9061 aaatttcagt tgctggcatt ttttgtgtgt atgtcaataa cattaactgg tatgcaccgt
     9121 aagtaataaa tgacagtgca actaagacta aaatatcagc aaagaacagt aagtacattt
     9181 gtacttttgc cggtagtgca gaaacaacaa tatcaacggt gaggtgtccc ctttcctttg
     9241 caacgaggat tattcctaga aagatcatat agacaaagca gattctggcg aattcctcag
     9301 accaagctat tccggagtca aaaaaatatc gtaaaaccgc attaaaaaag actaggataa
     9361 tcattgtaga tagagcaatt atcgcaagga gtttgagagt tctatctaat gctgtagaaa
     9421 tttgcataaa tttctccttc ttcatttaga tgggcgacct cttttatccg gtcgcctcgc
     9481 ttattgctta tttggctgcg tggatttttg aaataacatc tttagcccaa tcatatttct
     9541 gatagaaatt ttgataaagc gtttcggatg cttttaacat atcagcttta aattgttcac
     9601 tcggaacaat gacttctttg ccatttttgt ggaagaaatc ttttacatct tgttccatct
     9661 tcatcgctaa ttcccactct ttttcttggt agagttttgc tgcctctaag aagattgtct
     9721 gatcttcttt agacatttta ttccaagttt tcaaactaag ttgtagtaag ccaggtgaga
     9781 aaatatgatt agattcaata atatattttt gcacctcgta taaaccagaa tctttaatta
     9841 acatatatgg attttcttgc ccatcaatga ctttttgttc tagtgcggta aatacttcac
     9901 ttaaaggcat actttgaggg ttaatatgca ttgcttttgc ccagtccaca aacaacgaat
     9961 ttagtggaac ccttaatcgc aaacctttaa atccatcaaa attctcaact tttttattgg
    10021 aagagataac acggaagcca ttcataccat acgctagtaa tttaatgccg tacttctcca
    10081 ttttttcccc gatttcttga gcaataggag attctagaac tttcttagct tcggcattat
    10141 ctttaaatag atatggccac tcccatacgc caaaaattgg atcaacattt tgtagtaata
    10201 aacctgtaat gccaccttca atcgtgccat tacgcaaacc atttacaatt tgatcttctc
    10261 ctcccaactg gttatttggg aaaatacgga tttcgtagcg tccttctgtt ttttcttcaa
    10321 tggttttttt gaagtattcc tgcaatgcga tatttgatgg atgaacatct gaattatagt
    10381 gaccaatctt aatgatagtc ttcgcaaatg ccgaaccggt gaacatcagg ctagaaatta
    10441 atagagcttt agtgaaagtt ttgagtttca tagttattac ctcgtaggtg agttagtatt
    10501 taaaatacag cgatgatttt taagtaatca tcaggattag ctgagagttc ttcgatatag
    10561 tgaggtattt catcaaatgt aattacctta ctaatgatat cttttggttc aattcttctt
    10621 gatgccagaa gctctaatgc ttcttcaaat gcccccttgc ttgttcttgc cccataaata
    10681 ttaagttctt tgaaggtgat taaattagtt ggtaatggtg tttctgtttt cggccatccg
    10741 gttaaagcaa tacgtccagc aaaagaggtg tatttaagcg tattttgtac tgcgacattt
    10801 gctcctgacg cttcaataac cacctctgcc attcttccat tggtaattga tttaatagct
    10861 tcaagatcgt tttcttttgt tggattaatt gtgtgtggaa taccaataga ttttgcgtaa
    10921 tccaatcgct tatctaaaat gtcaattaaa atcggcgtcg ctccataagc tttggcaacc
    10981 aatgcagcca ttagcccaat tgcccctgct ccaatgatag caacatgttc accagctttg
    11041 acttttgttc tatgaattgc gtgtaaggaa atagttaagg gttctgctaa cgctaattcg
    11101 tgtattggca tatcaggtgt tttgattatc atatgggctg gatgtctaac aacctcttgc
    11161 attgccccat caatatgtac ccccaatact tttagagatt cgcaacaatt tgttctacca
    11221 atggagcagg gatagcaatg tccacaatag atgtaaggtt caatgatgac acgatctcct
    11281 acattaatat tatttggcat acctttacca gactcaatta ctgtgccaac gatttcatgc
    11341 cctaaaattc taggataagt aacaagtgga ttagtgcctc taaatgctcc aatatcagag
    11401 ccacatatcc ccataccttc tactttgatt agcacatcat tttcaccttt aacaggataa
    11461 gaaacggttt gtataccaac tttattaggt tctacaagac atactgattt gagtggtttt
    11521 tccatgattt tgtccttttt tgataaagtt aatcgacatt ttgtagtgtc aatggtattt
    11581 tatgcacaga tactaacacc aaaaaacaag tttggcattc caatttttgt gattttgtga
    11641 acctgatcac aaaaatgaaa ttttggaatt ccaatttcgt gaggatttat gcaaaatagt
    11701 tccagaactt acactaaaat aggaaaaatt ctcaaagagg aaattgctca aggaaaatac
    11761 agagtaggag ataagttacc tactgagaga gatatttctg aaagatttga agtgagtaga
    11821 actatcgtaa gggaggcgat agtgatgcta gaagtagaaa agcttattga ggtaaaaaaa
    11881 ggatcgggcg tgtatgtcat tagagtcccg gagctttttc cagaagattt tccagaagag
    11941 aaagataatg agctaaggga tgttgggcca tttgaactac ttcaagcacg acaattatta
    12001 gaaagcagta ttgctgagtt tgcagcacag caggctacca aacaagatat acaagtactt
    12061 cgtaacatat tagaaagtga gaagaaactt ttgttagaga agatggatga ctacagtgca
    12121 gataaggaat ttcatcttgc attagctcat atgacgggta acgatgttct gattcaatta
    12181 caagaacagc tttggcgata ccgctttaat agtgcgatgt gggctcagct acactccaga
    12241 attttacagc atgatcatca ccacttgtgg atagaggatc atgagcgaat tttaaatgca
    12301 atacagagaa aaaatcctgc attagctcgt aaagcaatgt ggcaacatct agaaaatgtt
    12361 aaaactaaat tgcttgaatt gtctgatgta gaggatccta aatttgatgg atatctattc
    12421 aatgtaaacc ccgttgtagt aggtatctaa aacctaaact acacaatgag gagtttacta
    12481 tggaacaagc atggcgttgg tatggaccaa acgatccggt atctttagat gatgtaagac
    12541 aagcaggggc aacggggatt gtgacagcgt tgcaccatat tcccaacggg caggtgtggt
    12601 cggttgagga aattaataaa cgtaaggcat tattagctga aaaagggctg gtttggtcgg
    12661 tggtagaaag tgtgcctgtt cacgaagaaa tcaaaactca aacaggcaac tatcagcaat
    12721 ggattgataa ctataagcaa accttgcgta atttggcaag ctgtgggatt gatacggtgt
    12781 gctataactt tatgccggtg ctagattgga ctcgtaccga tctggagtat gaaatgcctg
    12841 atggctcaaa agcattgcgt tttgaccaag tggcgtttgc ggcgtttgag ttatatatct
    12901 taaaacgccc gggagcggaa gccacttact catcagcaga acaagccgaa gccaaagcct
    12961 attttgagca gatgagtgac gctgatattg caaaattaac caaaaatatc attgccggtt
    13021 tgccggggtc tgaggaaggc tatacgctcg aagaattcca agcacagctt gaccgctatc
    13081 aagggctctc ggctgaaaaa ttccgtaccc atttagccta tttcttaaat gagattgtgc
    13141 cggttgcaca agaggtgggc attcgtatgg cgattcaccc tgacgatccg cctcgcccga
    13201 ttttaggctt gccacgcatt gtttcaacca tagaagatat gcaatggttt gtggaaactc
    13261 aaccgcttgc agccaatggc ttcacgttct gtaccggttc atacggcgta ttagccgaga
    13321 atgatttggt caaaatggca gagcagtttg cagatcgcat ttattttgct cacttacgag
    13381 ctacccaacg tgaagaaaat ccacaaagtt tccacgaagc ggatcatctt gccggtgatg
    13441 tcgatatgtt tggcgtggtg aaagcattac ttaccgaaga gtatcgccgc aaggcaaatg
    13501 gcgatcaccg attaattcca atgcgtccgg atcacggcca tcaaatgctc gatgatctga
    13561 agaaaaaaac caacccaggc tattctgcga ttggtcgttt aagaggatta gcggagttcc
    13621 gtggattgga gttggcactt aagaaagtct attttaacga ttagccttat ttattataat
    13681 gccctctact gtagagggca ttttatagtt tgatatgttc aaaaaatatt tcggcgaaat
    13741 aatccttttt ctcgttacct ttattgctgc agcggggtgg tttttctcta aacacgcttt
    13801 agttggatta ccgcctattg gctttatggg agggcgtttt atattagcct ctttgattct
    13861 tctgcctttt tcttaccgag agctgtatcg tttagataag gttcaagtta aatattcggt
    13921 gattaccggt cttacttacg gaatttatac tattttttgg gtattggggc tggcttttag
    13981 tacagtgttt ggtgaagggg cttttttggt gagccttgcg atgctgattg ctcctttgct
    14041 ctcttggttg atattcaaac atacgccacc gaagattttt tggttagcct tacccattgc
    14101 attaagtggg ctttactttc tttctgcgga gaaaggcatc tttcattttt ccttaggcag
    14161 tacgattttt ctggtgtcat cagtgtttgg ggcgttatat tttgtgttaa ataatcaata
    14221 cgccaaacaa gtctcattgt ttccattaat tactatccaa ttggcgatag tcggtgcggt
    14281 ctgtagcttg tattcctatt ttatggaaag ttggcatttc ccgattctca ccgaagtgtg
    14341 gctctggttt gccgcaagtg tattgattgc gacaaattta cgttttttaa tgcaaacact
    14401 ggggcaaaaa tattgccata ttggtacttc tgcactcatt atgttacttg agccggtttg
    14461 gacattgtta ttgagcgtga tgttattggg ggaacaactt agtgcgacaa aatggctagg
    14521 ttgcagcttg attttgatag cattgattat ttatcgactc cctattctac gactcaaaaa
    14581 ataggtaaaa caaaagcaaa tatgccatag tattggaagc agttaggcat tttagagtaa
    14641 gtaacaagcg gtcatttttg caaaaaatct tgcaaaaatg accgctttct ttcttttatc
    14701 cgttcaatct atgttaaaat tctacatttt tataagatga gagagagaaa cgtggaacag
    14761 aatttagtcg aatttttaac caaaacctta gatgatttaa aggcacaaga tattttagcc
    14821 attgatgtga aagggaaatc tagcattact gatacgatga ttttagccac cggtacatcg
    14881 agccgtcacg ttgcttcaac ggcagagcgt ttaattacgg aagcaaaaca ggctgggttc
    14941 gaggtatttg gagatgaagg taaagcagtt gccgattgga tcgtggtcga ttttgggaag
    15001 gcaatggttc acttgttgca atccgatagc cgtgagatgt accaattaga gaaactttgg
    15061 gcgtagcagt tcaataagga acgacaagcg gtgatttttg caacattttt tgcaaattaa
    15121 accgcttgta agtgaaagag agatgaaaat tcaattaatt gcggttggca ctaaaatgcc
    15181 ggcgtgggta acgacagggt ttgaagagta tcaacgccgt tttccgaaag agatgccgtt
    15241 tgagttggtg gaaattccgg caggaaaacg gggcaagaat gctgatacta aacgcattct
    15301 tgagcttgaa ggcaaagcaa tgttggcggc agcgggcaag gcaaaaatcg tgactttgga
    15361 tattccgggc aaagcgtgga caaccgagca gttagccact cagttagagg gctggaaaaa
    15421 cgatgggcga gatgtagcgt tattgattgg cggcccggaa ggattatcac cggaatgcaa
    15481 agccgctgcc gagcagagtt ggtcgttgtc gcctttaacc ttacctcacc cactggtgcg
    15541 ggtggtggtg gcggaaagtt tgtatcgggc gtggtcggtt accattaatc acccttatca
    15601 tcgagagtag tgagtcattt tagatagtgt tatatgtttg gtaaattagc aaaattcaca
    15661 gcttcaaacc agcgagaaaa ggtgcgagac gggcaggcag aaagcaatct gtatgcccgc
    15721 cgtgccttag ttgcgtttct gggggtgttg gcgttaagtg gtgttttatt aaccaacttg
    15781 tatcacttgc aagtggtgaa ttatgaggac taccaaaccc gttcaaacgg taaccgcatt
    15841 aaattgttgc ctgtgccacc ggctcgaggc ttaatttatg accgtaacgg taaagtattg
    15901 gcggaaaatt taacctattt cgggctgttt attgtgcctg aaaagacgga aaacattgag
    15961 cagactttaa ccgagttgaa ggatattgtt gggctgacgg acgaagatat cgagaattat
    16021 cataaagaga aaaaacgcag ttctcgctat accccaatta tgctcaaagg caatttgaat
    16081 gaggctcaga tggctcgttt tgcggtgaac caataccgtt ttccaagttt agatgtgcag
    16141 gcgtattata agcggaatta tccttacggc gagcttttaa cccatattat cggttatgtg
    16201 gcgaaaatta acgaaaacga taagaaaaag ctgcaagaag ccgggaaatt cggcaattat
    16261 gccggttcgc acgatatggg gaaattgggg attgaaaaat tctatgaaga tcagctccac
    16321 ggtgtcaccg gttttgaaga ggtagaaatt aataaccgag ggaaagtaat ccgtaaattg
    16381 cgtgaccaag caggggttgc cggtagcagt attcgcttga gtattgatgt ggaattacag
    16441 caatatgtga tgtcgttaat tgctaagcat aaaggggcgg tggtggtgct tgaccctcgt
    16501 gatagcagta ttttagcgat ggtctcaaac ccaagttatg acaataattt atttgtgggc
    16561 ggtatttcag ggcaggatta taaagcatta ttggacgatc caacccgacc actttatagc
    16621 cgtactacac aaggcactta tccaccggct tcaactatta agccatttat ggcggtggca
    16681 ggtttggaag agggaatgat taccccttct accaccattt cagaccccgg ctattggatt
    16741 ttgcccggca ctacacaacg ttatcgagat tggaaacgta gcggacacgg tgcaactaat
    16801 gtgaataaag caattaccga atcgtctgat acctttttct atcaattagc ctataaaatg
    16861 gggattgata aaatgtcgga ttggatgaaa aaattcggtt tcggagtgaa aaccggtatc
    16921 gatatttttg aggaatccgc aggggtaatg ccaacccgtg aatggaaaca aaaacgctat
    16981 aaaaaacctt gggtgcaggg cgatactatt ccggttggga ttgggcaggg ctactggatt
    17041 tcgaccccgt tacagttggc gaaagccacg gcggtgttaa tcaacaacgg gcgggtgaat
    17101 acaccgcatt taatgcttga agtgcaaagc agtaattcgg ttacgccgta tcaagaccca
    17161 ttgttgtatg aagatattaa aggcgtgccg gatgtctatt ggaatttagc aaaacaggga
    17221 atgtataacg taaaccacgc cgcaaacggt acggcacgaa aagtgtttgc cggtgcattt
    17281 tatcattcag cagggaaaac cggtacggct caggtgttta acttaaacgg taaaaattac
    17341 gataaaaatg cgattaaaaa agagttgcac gaccacgctt ggtttatcgg ctatgctcct
    17401 tatgaacaac ctaaaatagt ggtggcatta attttagaaa atgcaggcgg tggtggtagt
    17461 gccgcggctc ctgttgctcg tcagattatg gattatgctt tgagaaaagg cattggtgaa
    17521 cagccggatt cgcaaaattc tgagcaaaat aaaccgcttg tcacagagga acatcatgaa
    17581 tagtggcttt aaaaaacttt tctggaaaat tttttcactc gatctccttt tattatcagg
    17641 attattagct attaccggtt atggtttatt agtactatat agcgcaagcg gtgcaaatga
    17701 gcggatgttt accagtagac tgattcaagt ttcgctaggg cttgggctaa tgttgataat
    17761 ggcaatggta cctcctcgct tttatcaaaa agtttcgcct tatctgtata ttttctgtct
    17821 gatattattg attttggtgg atttggtcgg cgaaaccagt aaaggagcgc aacgttggtt
    17881 gaatttagga tttgttcgct ttcagccttc tgagattgcc aagctcgctg tgccactgat
    17941 ggtagccact tatttaggta agcggccgct tcctccgagt tttaaagata cttttatcgc
    18001 cttagccatt attattgtgc caaccttatt ggtggcaatt cagcccgatt tgggaacatc
    18061 tattttagtg tgttctgccg gtatttttgt tttatttttg gcagggctaa gttggaaatt
    18121 aattggcgca ggtgtggtgt ttttagccgg ttttatcccg ataatgtggt ttttcttaat
    18181 gcacgattat caaaaaaccc gggtgatgac cttaattgat cctaataaag acccgttggg
    18241 tgccggctat catattattc aatccaaaat tgcgataggc tctggtggca ttaatggaaa
    18301 aggctggatg gaaggaacgc aatcccagct tgaattttta cctgaaccgc ataccgattt
    18361 tatttttgca gtgttaagcg aagagcatgg tatgataggt gtgctgattt tattagcgat
    18421 ttatttgttt attattgccc gaggtttagt gattggcaca aaatcggatt ctgctttcgg
    18481 gcgtatatta tcaggtggta cagcccttct attttttgtt tacgtttttg taaatattgg
    18541 tatggtgagt ggtattttac ccgtagtcgg tgtaccatta ccgcttttca gttacggcgg
    18601 aacctcctat gtgacgctga tggcggcatt tggtttgatg atgtcaacct atgttcaccg
    18661 taagcgtgta ggtagtaata atccttatga taaactgaaa taaattaaac ttttgctttt
    18721 ttgcggtatc aaaaagcagt gaaacgatca tcgttacaaa caataacgac atccacatag
    18781 aggaaaaaac ttatatgaga ttaggtaaaa ttgtaaccct gttgttagct ggatttctga
    18841 catttagttc attaaattct ttggctgcga caaagaatac aaaagctaaa tcgagtattt
    18901 tagccaagaa gcaagcaact tctaaagcaa taaaaaaagt aggtatttct aaagttaaga
    18961 aaaaagaagt agtggctaaa gctacaaaaa agaaaacaat ttctactgtt acaactaagc
    19021 cgattaaatc tacaaaaaag ctggctcgca aggttgtcaa gaaaacggta accgctaaga
    19081 aaaaattggt aaaaaagcag cataagccac tggtcaaaaa acatttccag actggtgttg
    19141 ccagctatta tgcaaattac tttaatggtc gccgaaccgc taatggggag actttcaata
    19201 acgcaaaaat gacggctgca catcgaactt taccatttgg tactttaatt gaagtgacga
    19261 atttacgaaa tggtcgttct gtggtggtgc gtgtaaatga tcgcggacct tatgctcacg
    19321 cacgggtctt ggatttatca tcagccgcag cgaaaaaaat cggtatgcac cattcgggga
    19381 cggcaagagt gaaaattgcg attgttgata agtcaaaatt gggatcgact aatcagtttt
    19441 atgtaatgaa ttaatttaag agggaatatg aaaaaaacat tagtgaaagc gatgagcgtg
    19501 gtaggttttg cggtttcaag cattgcctat gcggatttaa acactaactt caatttatcg
    19561 gcaccccaga tcaacgcaca aacctatatt ttgatggatt acaactccgg tgaagtttta
    19621 gtgagcctaa atccggatca acgtcaatat cctgcatcat tgaccaaaat gatgactagc
    19681 tacgtagttg gtgaagccct aaaatcggga aaagtgcaaa acagtgatat ggtgatagtc
    19741 accgaaaatt cgtgggcgca aaaattcccc ggctcttcat taatgttttt agatttaaac
    19801 agcaaagttt cagtaggtga gttgatgaaa gggctgatta tcgtctcggg taatgatgcc
    19861 tctgtggcgc ttgctgaaca tgtttcaggc tcacaagcgg cgttcattaa tgaaatgaac
    19921 aaatatgccc aacagtttgg cttaaagaat actcacttta ccactgttca cggtttagat
    19981 gaccctgagc aatattcttc tgctcgtgat atggcgatta ttggttcgca tattattcgt
    20041 gatcagccgg aagagtataa aatttatgcg gaaaaagagt tccaatataa catcaaaaaa
    20101 ccacaaccaa accgcaacgg attattgtgg gataaaacca ttcaagttga tggtatgaaa
    20161 acagggcata ctgataaagc cggctataac ttagtcgcat cagcaattaa cccgaataca
    20221 cgtttaattt ctgtagtgat gggcgtgcca acttataaag ggcgtgaagt ggaaagtaag
    20281 aaattactgc aatggggctt tgcgaacttt gaaaccatta aatctattcc ggcagctcaa
    20341 tcggtggcag agcaaagtgt ctattacggt gaagtcaata aagtacaatt aggctcggtg
    20401 caagatagct ttgtgactat cccgaaaggt cgctcggcag atttaaaagc ccgttatgag
    20461 ttagagaaga aaaacctcga agctccttta gcaaaagggc aggtagtggg taaaatgatc
    20521 taccaacttg acggcaaaga tgttgcaagc actaacttac aagtcttaca agatgtaccg
    20581 gaagccggta ttttcggcaa agcgtgggat tggattgccc ttactgtgaa gagcttgttc
    20641 gattaatctt gttatttgcc tttttatccc catctaggga gcaatgcaag cggtgtgatt
    20701 ttgcaaaaaa tttgcaaaat ttcaccgctt accgttttag atatgaatgt ataaaaagtg
    20761 tagtggcgaa aaatttttcg cccctcgaag aaagaataga agaaggatca aaaatgtctc
    20821 aacccaaagc gataaactta caagatttac ctcaagccaa attaaaagat ctgctagagt
    20881 ttccgtgcca tttcacgttc aaagtggtgg gagcaaaccg agaagatttg gttgatgatg
    20941 tggtagtggt cacgcaaaaa tatgccaaag gcgattacaa tccacgtcag caaaaaagct
    21001 caaaaggtac ctataattcc gtttcgattg atattgtggc ggaaaatatt gagcaagtag
    21061 aaactctcta cacggaactt gccaaaatta atggcgtaag aatggtatta taatgcgagg
    21121 ggcgaatgac attcgccctc aatttttcaa taaagcgatg ttggaaaata gaaaatgaaa
    21181 ttaataattc gccaactagg cattcagcct tatgaagaaa tttggcacca aatgcaatct
    21241 ttcacagata gccgtgatgg gaataccgat gatgagatct ggctggttca gcacccggct
    21301 gtgtttacgc aaggatcggc aggcaaaccg gagcatttgt taaatccaac caatattccg
    21361 gtggtgcaaa gcgaccgtgg cggtcaaatt acttaccacg gcgaggggca gcaaattatg
    21421 tatgtgctga ttgatatcaa acggctgaaa tccttgggta aagaagtgtc ggttcgagat
    21481 ttagtgactg ccctggagca aaccgtggta caaacgttgg cagaatatgg catagaaagt
    21541 tatccaaaag ccgatgcacc cggggtgtat gtggacggca agaaaatctg ctcacttggt
    21601 ttacgaattc gaaaaggttg ctctttccac ggtttggcat taaatatcaa tatggactta
    21661 acgccttttc aaaacatcaa tccgtgcggt tatgcagggc tggaaatgtg ccaattaagt
    21721 gaactggttc atcgccctga tttgcgttgc gaagaggttg ctccaaaatt agtggctcat
    21781 ttcagcacaa ttttaggcta taataaccaa caaattatta acaattagga taaaacacag
    21841 taatggctac atcgacagct caaacgacgg caactgctac agtgaaaaaa atgagccctt
    21901 tcaaaatgga aaaaggggtg aaatatcgtg atgccgccaa aacctctgtg attcaggtgc
    21961 gtaatatcga cccggatcaa gaactcttaa aaaaaccgga gtggatgaaa atcaaactgc
    22021 cggcaaattc ggcaaaaatt gatagcatta aaaacggtat gcgtcgccac ggcttgcact
    22081 cggtgtgcga ggaagcctct tgcccgaatt tacacgaatg ttttaaccac ggtactgcaa
    22141 cctttatgat tatgggggca atctgtaccc gtcgttgccc gttctgtgat gtggcacacg
    22201 gcaaaccgct tccgcttgac ccggacgaac ctcgcaaagt ggcagaaacg gtgcaagata
    22261 tgaaactcaa atatgtggtg attacctcag tggatcgtga tgatttgccc gaccgtggtg
    22321 cggctcattt ctctgcgacc gtgcgtgaaa tcaaagcctt aaatccgcat tgtaaggttg
    22381 aaattttagt gcctgatttc cgtggacgtg tggagcaagc ggtggcaatt ctcaaagaaa
    22441 atccacctga tgtctttaac cacaacctag aaaatgtgcc acgtctctat cgtgaagttc
    22501 gtccgggagc ggattacaaa tggtcgttgg aattattgaa aatcttcaag caagagttcc
    22561 cgcatattcc gaccaaatcc ggcttaatgg taggcttagg tgaaaccaac gaagaaatct
    22621 tggaagtaat gcaagattta cgagaccacg gcgtgacgat gctcaccatc gggcaatact
    22681 tacaaccaag ccgccaccac ttaaaagtgg aacgttatgt gccaccggca gagtttgata
    22741 tgttccgtga ggaagctgaa aaaatgggct ttgaacacgc agcctgcgga ccgttcgtgc
    22801 gttcttctta ccacgcggat ttacaagcga aaggcgaatt ggtgaaataa aatcgataag
    22861 ggcgaatatt atttgccctt tgtttttgtg gtttgcaaag ttttagcaaa attaaaccgc
    22921 ttgtaatctc ttctaattta ctcttctata gataatttcg ttgttgcgaa ttattttcat
    22981 tgactatttg ttatagaaag ttacaattca actttccatt tctttttgga gttccaatga
    23041 aaaaattact attaaacagc gtatttgctg ccacattgct tgcttttggt gcaactgcca
    23101 atgccgaaat taaaaccatc actgatgtat tagggcgtga ggtcaaagtt gatgtgcctg
    23161 ctaaacgtgt tgcactgact ttctattacc ctgactatat tgcggtaacc ggtgttgaaa
    23221 atttcgataa ggtagtaggg atttcacgag agttttggga aaagttcaac ccaggcagtt
    23281 gggcattatt tgcagaaaaa atgccaaatc taaaaaacat tgccgatctt ggctatgtga
    23341 atagtggtac tttttcaaca gaaaaaacca tcgcattaaa accagatgtg ttagtgctac
    23401 ctgaaattca atatcaagcg ttagccagtg aaatttcacg cattgagcaa gctggcattc
    23461 ctgttgtagt agatttcaac aagcaaacta ttgaaaacca tacaaaaagc gtgagagttt
    23521 tcggtcagct tacaggcact gaagagagag cggagaaaat tgccaaagag tatgaagagg
    23581 gcattgctga tattcagagc tgtattgata atgcaaaagt aagtaagccg aaaatctatg
    23641 ctgaatttgg taataaaggc ccgaaagagc atagttttac ctttggtaaa aatatgtggg
    23701 gagcgattgc cgaaacggtg cgaggcgata acattagtgc cccgtttgta gaaaactggg
    23761 ggcagattaa ccctgagcag gtgttagttt ctaaacctga tgtgattatg atttcaggta
    23821 cggaactagg caacgacact aaccctgaga ttatgtcgat ggggattaat attgaagaag
    23881 ccgatgctca aaaacgctta aaaggcttta ttgagcgtac aggttggaaa gagttacctg
    23941 cggtaaaatc agggcgtgtt tatggtttat atcatacagc ttctcgctca atttcagatt
    24001 tagcgagtgc tcaatttatg gcaaaagcac tttatcctga attgtttaaa gatatcgatc
    24061 ctgaaaaaac gtatttggat ttccacaaca actacttacc aattacgcca aaaggcacat
    24121 tctttatgca actgaaataa aaataacaag cggtcatttt gcagatttat tttgcaaaat
    24181 gaccgcttgt attcgctttt actatttgcg gttcaaacag caatcaatat ttaaacattt
    24241 gcgaagcggg cggataatcg gcgagccgtt tccatcatat aaaatctcaa tattgacatt
    24301 atagagtctc tgaataatat cgcattccaa catatcacaa gctctgcctt gtgcttgaat
    24361 ttcccctcgc ttaatagcat cacactatcc gaaaattgag ccgcaaggct taaatcgtgc
    24421 agcaccataa ttgtaattag gttattcgct cgagtgtagg cattcacata ttcaagtaag
    24481 ttgatttgat gatacatatc caacgcactg acaggctcat ctagcagcag aatgtgtggt
    24541 ttacgtagca atacttgtgc gaacattacc atttgccgtt gcccgccact tagccgcata
    24601 atatcttgat gagctagatg tgaaataccg agtttatcca taatatcaac ggcttcattg
    24661 agtaattcat cgctaatata catattcagg ctatcaagtt gccctaataa aataacctct
    24721 aaggcactga gtgaggcctc tacctgtgtg tcttgtggca tatagccaat ttctttacgc
    24781 caactatgca agtggcttga ggttaattta ttaccgtcaa aatcaattgt gccttgatga
    24841 gcaatttccc caaatagagt tttaaggaga gaagatttac ccgcaccatt tgggccaagt
    24901 aaagtataga tcttgccttg ttcaaaatgt agattgattt tatcagccac cactaa
//
LOCUS       DANLZC010000018        24317 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.018, whole genome shotgun sequence.
ACCESSION   DANLZC010000018 DANLZC010000000
VERSION     DANLZC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 24317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24317
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.018"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(211..1299)
                     /locus_tag="PXE44_000572"
     CDS             complement(211..1299)
                     /locus_tag="PXE44_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5588113.1"
                     /translation="MGIMSKSISIFNHKGGIAKTTTAFNVGWSLANQGYQVLLVDLDS
                     QCNLTGLVLGYDQCKEDSDLALFYNNRHHLTMEGIVEALINGQSPDNYLSGNVGQITQ
                     TLNPNLFLLPGHLNVSELDSQISVSLKIAAGVPATRNIPGNLPTLIKKVAEQNNIDYI
                     LYDLSPNVGGLNEVILMSSDYFIVPTSPDFFCWQAVGSLAKNIRKWHNELKAFKELNG
                     FNQSSYAIKNKPQFLGTIQQRYRPRKGQPAKSFEKWIERIRNEVITNFVPVLKTIDCA
                     IDDTAIYAALKSTNSDLEPFDLAHIADFNSLIAISQELRVPVFSLTKEQIKNSGQFGH
                     ALNTMDESKENFDQEFQSLAERIIQLTN"
     gene            complement(1364..1795)
                     /locus_tag="PXE44_000573"
     CDS             complement(1364..1795)
                     /locus_tag="PXE44_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588114.1"
                     /translation="MKKMAIVALSAFFSMNAFANNNVVYSCTTTDNQTLKVTKEGGNY
                     VYSHGNTTFKNPVKEALKNPASEIAGGSQFTTISLELRNAGKSYIVGHIEADPKSPFE
                     ASVQIQDIKTGNDITSFECRSDKPIRHNFDRKLMRKSGFAA"
     gene            complement(1799..3901)
                     /locus_tag="PXE44_000574"
     CDS             complement(1799..3901)
                     /locus_tag="PXE44_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5588115.1"
                     /translation="MKKAKSLKFLTACIMLTTMSGYANSNTVNVGNSISNFIENTTGY
                     RTGNLGVSGNNALTEYYPVLSDYMHYSIGGGSAISAPASRGRPNQYGIGIGWKSNLTC
                     GNFDIKLSIKNQLNGITEGFKDLYGNIIESATGAVASLPAMIIQRANPQLYDILSNGL
                     YQGKLDFNNFKTSCEAMSNQLADLAMDSKLAKMAGLHRAAEIAETEPDAMAAKKKMDK
                     KDAEKGIPWVGGEQRGGKNQKPMAIVADIVGAGYNLALGRGPLEKGDIPIASCDGMLC
                     TEWKNPSEAAAYASEVIGETFLDACETCEQSSSGAKAGKGIAHFIEQTTIEKATKLEA
                     ALNHLDSLTPEQLNSLSTSTVSVTKGVLEAIAASPDAALLGARLSQELAISKEMEKLL
                     LIRRAMLAGMREPNVIKSSLVEEAERNLKLLDREIEQVKLEMELQRSINGNTALAAIT
                     QQMNEQALAIDDNAMGDGNRRFANLSNQAGANNLNTSGIANSAQNAVGDRYIAIPKSD
                     GSGMSDFYGAYKASPTNIVSNVNVGSYTQIAQMNGNANEQATALIKQFEGYLSHGKWD
                     VNANRAGYGSDTYTTADGMVHRVQKGTYVSREDAERDLARRMPQFMNVARGNVGADVW
                     AKLTPQAQAALTSVSYNYGSLSKLESVQKAAAVSAQSGDMTALANSIRNLQGHNNGVN
                     ARRRNQEADYIINAPKGN"
     gene            5176..5607
                     /locus_tag="PXE44_000575"
     CDS             5176..5607
                     /locus_tag="PXE44_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL5588116.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEHKKGNPFYACRVAAL
                     VGESDSPEYRYFDMNVVGKETETLIKKCQKAVSEEKKVLVSFVMADLWFDTFTYSSDS
                     KYHKKGDTGVSLKGRLIRIKMIKIDGEIVYQEKPKADEQSE"
     gene            5713..6393
                     /locus_tag="PXE44_000576"
     CDS             5713..6393
                     /locus_tag="PXE44_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588117.1"
                     /translation="MNSLQQFVKTLSPTVLNGRHFDGIVGDVVFVQNGYGKWLDQGFT
                     IIGFDKSIEEKDWIYLDWDCYWFGKNPAQVIPEKSWAQRSWLARAYSISLERFNSLGL
                     EREYNIVSDWDNPIYGTDIQLIEHSKEFFRWTEADDRGIFLVHNEMAFHFFKKEIKAS
                     SEPEYYQPIETFWKDATVVLNIEDLDMLERVGLEQTNTPALAKLTTFVQAARNEISNG
                     NIVFYRAF"
     gene            6419..6826
                     /locus_tag="PXE44_000577"
     CDS             6419..6826
                     /locus_tag="PXE44_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL5588118.1"
                     /translation="MNRQGIYINGKQVFDSKGKEINSVQIINSTVQTVVGINKGYISM
                     AAGNNVLIVDGQTIALEPSPTINIEVFGNVTNLNAGSENVRVTGDAKSVSTVSGDVTV
                     DGNVTSNVKTVSADIHCKSILGKINTVSGDIYR"
     gene            6906..7268
                     /locus_tag="PXE44_000578"
     CDS             6906..7268
                     /locus_tag="PXE44_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588119.1"
                     /translation="MEDSFFDFDDISCYLGQWEAILEEYSDIVSIEDFWLVAKEFETV
                     PHFGNLYQELVISRLIQRFCTELDIEQDSDLVEFDYYINAIDTHFYINRQRICDIDDW
                     NEMLDKIRKEMTPAKLAA"
     gene            7381..7641
                     /locus_tag="PXE44_000579"
     CDS             7381..7641
                     /locus_tag="PXE44_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588120.1"
                     /translation="MTFELGRVCTTRAVAECFNQNALVELLNRYCYGDWGNLCEEDKL
                     ANQEAMKYGGRIVARYDVDDESLYIITEADRSCTTILFSYEY"
     gene            7800..8429
                     /locus_tag="PXE44_000580"
     CDS             7800..8429
                     /locus_tag="PXE44_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588121.1"
                     /translation="MKTKISLMTVFMLGLSGCVHQQDTAQNSDEITPMPDSLLTPQMQ
                     QLPPQDRIVSKDIYQEQTTSNTEVVRDGRYTLISIAPEEGQKYLLEQVVSVKVSNPKN
                     RFTANVQQGLRSTLTNTGLDLCYPPIGEQGNAVQTLFTRPLPKVHYQFGPMKLYEALQ
                     MIAGPAYELTLNDISRTICFKARESSFQNPHKPAVAVTTTTIKTEVIEE"
     gene            8429..9202
                     /locus_tag="PXE44_000581"
     CDS             8429..9202
                     /locus_tag="PXE44_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HDL5588122.1"
                     /translation="MREISMKFNLCRKAIPLAVLPLLLSFALPTLAQNMQTETVSSSQ
                     STLQKQSQHQQSQLSDSLKQAKEWGLTVEEWSRYTELMKGERGMWSPNLDPLTALGIE
                     ARTAEEREKYARMLAKKYYERVSKELDFDKVYRQEFEKLYPNELPFEVEPHISQSVGR
                     VVYFTRFDNCDSCKSDLGKILSYANNQTPLDIYIVGQNISDNDIRKWAADNNIDPIKV
                     SKRLITLNHDKGYWLRYSLGKMPAAYQIKQDGEWTSLVY"
     gene            9181..9951
                     /locus_tag="PXE44_000582"
     CDS             9181..9951
                     /locus_tag="PXE44_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HDL5588123.1"
                     /translation="MDITRVLVIVAVFLSNISISQAKPFNGFGSEIDSYIRMAAQRYQ
                     VSEAMLRGFVKMEDGWYGNISPTGATGVGQFTMGTWNWLSETERGKQLGMKLITAKNR
                     GSYADPRRDKYTNTLATALLARWHIEQFQLRNIPITDENLYMAHNIGLDGLHRAMMGK
                     ATKEDILNMRRNGMKSWMTVRDFLNYQKGRFLAHKQEANFMTVSKQGHIHTGVAAFVY
                     PTSQMGKSVKWVEPDNKMIWVEPKNAMVWVEPQNTLQR"
     gene            9954..10460
                     /locus_tag="PXE44_000583"
     CDS             9954..10460
                     /locus_tag="PXE44_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5588124.1"
                     /translation="MQNKVKVLLFSLYVSHFSWADLTVIADFGGESAVRFYESLQPEH
                     DENAPNYPNSISASVSEADILPIVSHKLSPGTIKEKPFDLTGMQAIFLIGADNTSAQW
                     LQQNREKLITLNATGLVVNVQTLSELNQLRDIVPELRILPTPGDDLAERIGIAHYPVL
                     ITESGVSQ"
     gene            10460..12661
                     /gene="traD"
                     /locus_tag="PXE44_000584"
     CDS             10460..12661
                     /gene="traD"
                     /locus_tag="PXE44_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDL5588125.1"
                     /translation="MSAKHTLEAWLRPPVEFFPAAVHASCAFVSAAAPWSLALHPSIG
                     YSIAAGFGALSFYRAKQGWQIVRYHRNLKRLPNYSMTSKQIPVSNKYLFVGKGFEWKP
                     IHTQRLHDCFSDNGSKYLEPSKLFKMAREYEKRHENWLTHLTSKDSRWNLVRPLPPVE
                     GIPAIHGIEPDEVDVVQSVSSRVGHTVVFGTTRVGKTRLAEVFVTQDIHRGKTADERE
                     VVVFFDPKGDADMLKRMYAEAKRAGREDEFYVFHLGYPEISARYNPVGRFNRISEVAG
                     RISGQLSGAGNSAAFKEFAWRFVNIVARALVELGNRPNYVQISQFVQNIDSLFLDYAE
                     KFIRKTDPKIWENLATIAAGLDEKALPFGMKDRPLIFVINKYIAESAVFDPILQGLAS
                     AVRYDRTYFDKIVASLLPLLEKLTTGKISELLSPDYADILDERPIFDWESIIRKRGIV
                     YIGLDALSDATVAAAVGNSMFADLVSMAGHLYKFGTNDGLPGAFKEKSKPVKINLHCD
                     EFNELMGDEFIPLINKGGGAGIQVTAYTQTLSDIEARIGSKPKAGQTLGNFNTLYMLR
                     VKEPATAELLTKQLHEVTIKRSTVTSSTTDSSNPDDDKAFSSNTGQRISESTVPMLVP
                     ANITNLPKGQAFALMDGSKLWKIRMPLPAVDKDDMMHQSLRELTDYMRKNYHVVENWW
                     QPAFTEYTPSRDIAEAFEAMSEPQYKTQIDHQVDNFGIDEPYDIDEEENDE"
     gene            12654..13007
                     /locus_tag="PXE44_000585"
     CDS             12654..13007
                     /locus_tag="PXE44_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5588126.1"
                     /translation="MSSAKMREEKRTNLLDLPNKYRNFNGEFSASCGLDNAEELLIHS
                     QSYFIEWFEQGYSFHQFAEKFADQGLSLWSADEVSMRHSDKSKDIFAFYLAFDNNPSG
                     YILVQCQLDREDSLQ"
     gene            13004..13699
                     /locus_tag="PXE44_000586"
     CDS             13004..13699
                     /locus_tag="PXE44_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HDL5588127.1"
                     /translation="MSEETKARRKSGLINLIFGKINMSLAAIAVSLIFSIILEWIGIA
                     FWWPQEGHLHSQTMMLNEMQWLSDDFSQSLFAKSSTAFATSIIENVYNWLFVYTGVKE
                     WLTSPGNGSMEQWVHHYGRAYIESVIYVVITFIIRLIIIVFTSPLFLLTALSGLTEGL
                     SLRDIRKFGAGRESAYLYHHARRYIMPMMVTAWVLYLSIPFSIHPNIILVPAACLFGL
                     SICITAASFKKFL"
     gene            complement(13731..14114)
                     /locus_tag="PXE44_000587"
     CDS             complement(13731..14114)
                     /locus_tag="PXE44_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588128.1"
                     /translation="MRYQMRYLALFLVVFPYVAHSLPSAQKVGNICHAENAQYNYGGL
                     WELSLRGKLICNYWQFLGDDERKKIELQLDEYGEFPIWNTSEQISEQIIEDDEYPQYW
                     EVEDWEDSDWDTESENEDTEATTEE"
     gene            14301..14627
                     /locus_tag="PXE44_000588"
     CDS             14301..14627
                     /locus_tag="PXE44_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HDL5588129.1"
                     /translation="MSISTSLRKGIVVLVTLSCLPALASTEQEQLAQAIKQLEAAKLS
                     LQRAEKVAKASPKTREYFNYSAIHSDIETVKKGIQQYINPSRALPRDPNVLRTLSDDY
                     SKLRGQ"
     gene            14627..14860
                     /locus_tag="PXE44_000589"
     CDS             14627..14860
                     /locus_tag="PXE44_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HDL5588130.1"
                     /translation="MSYSPANAFSVASGIDPFRLKIMIAIIFLSLLLLAVAWALNSGY
                     KGFSFRDKSPFSLLVLFLKGVALLACVTYFFIY"
     gene            14875..15264
                     /locus_tag="PXE44_000590"
     CDS             14875..15264
                     /locus_tag="PXE44_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2976 domain-containing protein"
                     /protein_id="HDL5588131.1"
                     /translation="MNRIKSLTKHLHSRYLAVMLFFWGSLQNAFAAGPDASKIPDFQI
                     PGVDSNTKDPMEIIWIVLKGLVTLGAVILAAVAILTVVKALIGIYNEATSENGKKGWG
                     HFILALIIGFIVIFFCLWLVKLAVGLF"
     gene            15287..15652
                     /locus_tag="PXE44_000591"
     CDS             15287..15652
                     /locus_tag="PXE44_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HDL5588132.1"
                     /translation="MSEQDTTIPFLPTRLNREATVYGGMTVSEFGISAGLGFMLGLIV
                     GLFLWILTDFWLLIPAMAMLLCIATVLIGKGIVAAVKRGKPEAYLNRLVEKKMDDLFN
                     GHKFIKREGFWSIRRYRRK"
     gene            15656..16300
                     /locus_tag="PXE44_000592"
     CDS             15656..16300
                     /locus_tag="PXE44_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03746 family integrating conjugative element
                     protein"
                     /protein_id="HDL5588133.1"
                     /translation="MSQLKSQLHEKDKHIKTTRIFAFLFFIIILVLAGVIYTFPSRLT
                     IYNPPDLRAGSQRAWWEIPKPTIYAFSYQIFQQMNRWMTNGEEDYEKNITTLKPFLTP
                     HCEAFLRQDYKDRLRNNELRSRTRMVYEITGRGYSEDKVTIFSEDSWAVNLDLSVDEH
                     FQGQPVKRVYIRFPISIVRMDVDSAQNPWGLGFNCYHSIPKLLEGVEPKTEEKK"
     gene            16300..17184
                     /locus_tag="PXE44_000593"
     CDS             16300..17184
                     /locus_tag="PXE44_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HDL5588134.1"
                     /translation="MKRLPILLVSLLIGFSGSSLADVLMKWERKPLPVALQVEKERII
                     FVDKNVKVGYPAELDGKLRIQSTGGAVYLKALETFPSTRIEFRDVTTGEIFLFDLTAA
                     SKVANPEEAIRVVFDGTVEKQSFSSNRDDESSDLPIANSRPQLPVPAALTRYAAQSLY
                     APLRTVEPLDGVRRVAHRLPKKITKLLPAYSIEATPLISWQLDEYVVTAIRLQNKGNT
                     QITLDPRELQGQFYAATFQHNWLGGYGENEDTTTLYLVTEGYANNALIPETRPVKLVS
                     KKVKRTTKVETKPAQSAK"
     gene            17196..18650
                     /locus_tag="PXE44_000594"
     CDS             17196..18650
                     /locus_tag="PXE44_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL5588135.1"
                     /translation="MKANKALFVILGIVFILVGGIVYALFGRSESVPQPTQQEILDIS
                     IKDLTPEQVRTMGIQGDTPIDTLRTLVTTVKSNERRMDELIDENRQLKQKEDLVNGRI
                     NDAVNNETRKLQEQIADLKMQLFDMATLVNNKPTTQESTPQVEAQPNTVPNKGFGTAG
                     DKLPIGGATMSNVDTSGMYWVEPSDQISLDASGKPVSGNTVATKFGFPKDFNGGQSAG
                     NNEKKGLPFFPETDTVTSSQGTAGNARGKNQMTSVYTIPSNSTFLDSVAMTGLIGRIP
                     IDNRVSDAYPFKVLIGRENLIANGIELPDVDGAIVSGTATGDWTLSCVRGSVKSMTFV
                     FNDGRIVSTSNKGNGEEIGWLSDSSGIPCVPGERKTNAPEYLGTNFFLAAASAAAQGF
                     SQSQTTTVVDGSSVVGAVTGDQGKFILGQAIGGGLKESADWFRKRFAENFDAVYVPPG
                     HRVSIHIEKEIEIDYNAQGRKVKYGSKSTNSQLD"
     gene            18663..19061
                     /locus_tag="PXE44_000595"
     CDS             18663..19061
                     /locus_tag="PXE44_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HDL5588136.1"
                     /translation="MKHLFCIMISALLITGCTTSTEELLPTNGSTMRDIWDKGASNND
                     LHLYRSQQGRTLDPVDYIPNSEQRAYTRTAENEINNLFPRLPNPDLIMYVYPHQTRSG
                     EPLSIPGYSTVLPFYGRVQYAQPGDRTRGL"
     gene            19064..21901
                     /locus_tag="PXE44_000596"
     CDS             19064..21901
                     /locus_tag="PXE44_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HDL5588137.1"
                     /translation="MSFLSEVFSSFKPTKAGVISSEPNENTGESDIIQNKMSEVNNEV
                     QQLKRSGKLTQSQHHRRLYSQEPSFIDHLPWGEYLNEHGVILLDDARSVGAVFDIKPI
                     GTAGRSIEFLSRIRNLVKDALQDSFDELDVNPYVVQFYCQDSDDLQPYIEQLRNYISP
                     IAKNSAFSEEWLRVQAKHFKDIGKPEGLFIDERVTNTAWSGRIRSTKMVIYRYVDKSN
                     DTNENLIVEQLNNACSRINGALTTAGLQLQRLDGEQFRQWLLRWFNPNPSINFEGLSN
                     NDCYHLFGKQTSNDGLPELAFNDFSEDLFYSQPKNEGNYWYFDDQPHEVVVVNNLRKQ
                     PEVGLMTGEITRGRNINALFDLLPESTIMAITIVIHPQDKLEAHLEKISSRAVGENFE
                     SKYLKEDCQTVQSYLKEGHKLYQAAIAFYVRGKDEKELRTRSRQLRTILLSNNLEPVK
                     ENAEIAPLNSYLRWLPMNFNPQMDAKSHYYTKYYFVQHLANLLPIFGRDTGTGNPGIT
                     YFNRGGAPFNFDPFNRKDRTKNAHKLLLGPTGSGKSATLCGQLCQAMAVYRPRTFVVE
                     AGNSFGLLADYAERFGLTVNRITLAPNSGIVLPLFSEAHRLLDMELNPEDVPEDIEDN
                     DDTDDEGDMQRDLLAEMELTARLMITGGEKKEDEKMSRADRAAIRRAILNAARQTKSE
                     GRQTLTEDIKNALEALSHDEHTREERRTRLAEMAESLEMFCTGVNGQFFNREGAIWPE
                     ADLTLVDLAMFAREGYEAELAISYISLINHINSLGEKYQHSDRMILNVTDESHIITAN
                     PLLAKFLVKGSKMWRKLGIWLWLATQNMDDFPNEAAKLLSMVEWWELLNVSASEIDEI
                     NRFRKLSEAQKTMLLSAKKSDRKYTEGVVLATNMEALFRVVPPSLFLALGMTEKHEKA
                     ERKQLMMQHGYTELDAALEVAARIDKARGIH"
     gene            21901..22329
                     /locus_tag="PXE44_000597"
     CDS             21901..22329
                     /locus_tag="PXE44_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588138.1"
                     /translation="MNKQHYRLFNAGPQKSSLGVVSPAVETQLLGFCEQIRQLIGVGK
                     QITLFPHRVVQNHWAIELSGEQGSVHLLLNVTGRSLLPTSNNSEWSARLIIEIADHVD
                     AYWFSAFLTQYLGLKSNLLPLSTSIADNSVYNEVIDNNEV"
     gene            22319..22636
                     /locus_tag="PXE44_000598"
     CDS             22319..22636
                     /locus_tag="PXE44_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5588139.1"
                     /translation="MKSDTTKPKMTRVITVLKITLMIIGIILLLLVVGWLAMTYFPST
                     NFQAWFYETRYAWLGWRFVLYVAIWLLMVQLNRKGQMPLKARLMILSVIPIIEGLNLL
                     YLL"
     gene            22647..24119
                     /locus_tag="PXE44_000599"
     CDS             22647..24119
                     /locus_tag="PXE44_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL5588140.1"
                     /translation="MTFTVDSYLEYFLTLLAWIINNNIFAVLVQTGLFVLPLIFVLIS
                     TWMEVKKQGEDEGNKGDLLITWLSLKFYPAMFVVVLVLAPMIPINLNNIELNVERSKA
                     CGYRVPTAPEDSGYNQMNSELGDQQARVPIWWGLFHQINKGVTHALTASIPCKPDLRQ
                     VRFEVQHEKINNPALLTEVRLFVQQCYVPARQKIQNSQLSLSPAQVRETSWLGGRILI
                     ANSELYPRYRAQQPHSLFPYNASRDNGLPNNGEGGFPNCAEWWDTANNGLKDRLLDDV
                     KQNFSAKVIDFFSSKENSDESILRTVLRAENMDISRGKVYPGYGGNLDRSFTNEVTKT
                     ASMFGLSLGSIGAFPAFDSMRQALPMVHSFALMALIILTPLIMVMSGYSLKTLVTLTF
                     VNFALVTLTFWWELARWLDSWLLSVLYDSGTHSRINPYFLENTQDDIIVNFVMGSMFL
                     VLPAIWLGSMSWVGVKMGEFASHLTTGSRDSKTAGSKIGK"
     gene            complement(24165..24305)
                     /locus_tag="PXE44_000600"
     CDS             complement(24165..24305)
                     /locus_tag="PXE44_000600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588141.1"
                     /translation="MANSEISSKDTDLKSLFSDDEEQEDDEYQYGYDGPGIYLGNYRV
                     DE"
BASE COUNT         7303 a         4627 c         4997 g         7390 t
ORIGIN      
        1 atactatatt gcagtgtctg cattttgctg aagagaaggg agctataccg cctatacctg
       61 aagattggcg gtatgaaatg acggggcgta gtatggggat tgagcagatt ccaaatcaga
      121 aattatagct gggcatttac aaaatacaca aaagaaagta tggtgtggct aaattttagc
      181 taaccatact tttttattat gaataattgt ttaattagtc agttgtataa ttcgttctgc
      241 taatgattga aattcttggt caaaattttc tttactttca tccattgtat tcaaagcatg
      301 tccaaattga cctgagtttt taatttgctc ttttgttaag gaaaatacgg gaactcgtag
      361 ctcttggcta atagcaatca acgaattaaa gtcggctatg tgagccaaat caaatggctc
      421 taagtcagaa ttcgttgatt taagagcagc ataaatagct gtatcatcaa tagcacaatc
      481 aatagttttt aataccggca caaaattcgt aatgacttca tttctaattc gctcaatcca
      541 tttttcaaat gatttggccg gttgcccttt tcttggtcga taacgttgct gaatagtacc
      601 taaaaattgt ggcttatttt taattgcata agatgattgg ttaaaaccat taagttcttt
      661 aaatgctttt aactcattat gccatttgcg aatattttta gcgagcgaac caactgcttg
      721 ccagcagaaa aaatcaggtg atgtaggtac aataaagtaa tcactagaca ttaggataac
      781 ttcatttaat ccacccacat ttggactaag atcatagagt atgtaatcaa tattgttttg
      841 ttcagctact ttcttaatta aagtcggaag attgccgggt atatttcttg ttgctggaac
      901 accggcagca atttttaatg atacactaat ttgcgaatca agctcggaaa cattaaggtg
      961 accaggtaac aagaacagat ttggattcag ggtttgagtg atttggccaa catttccact
     1021 aaggtagtta tcagggcttt gtccgtttat cagtgcttca acaatacctt ccatagtcaa
     1081 atgatgtcta ttattgtaaa aaagcgctaa atcactatct tctttgcatt gatcgtagcc
     1141 aagtaccaga ccggttaggt tacattgtga atccaaatcg actaacaata cttgatagcc
     1201 ttgatttgct aaactccaac caacattaaa tgcagttgta gttttagcaa tacctccttt
     1261 atgattaaaa atactgatag acttactcat aatacccata attctatgaa ataattaacc
     1321 caccgaactc atttgagaat ggtgggttaa tgctttgctt tagctaggct gcaaatccag
     1381 attttctcat cagtttgcga tcaaagttgt ggcgaatagg cttatcggaa cgacattcaa
     1441 atgatgtaat atcatttcct gttttaatat cctgaatctg aacacttgct tcaaaaggtg
     1501 atttagggtc tgcttctata tgacctacaa tataagattt tcctgcattt cttaattcga
     1561 gcgagattgt tgtgaattgt gaaccacctg caatctccga tgccggattt ttgagagctt
     1621 cttttacagg atttttgaat gtggtattac cgtgagagta cacatagttt cccccctctt
     1681 tggtaacttt taacgtttgg ttatctgtag ttgtgcaaga atacactaca ttgttatttg
     1741 caaaagcatt cattgagaaa aatgctgata aagctacaat tgccattttt ttcataggtt
     1801 agtttccttt tggtgcgttg ataatgtaat cggcttcctg gttacgtctt cgagcattaa
     1861 caccattatt atgcccttgt aaatttcgga ttgagttagc taaagccgtc atatcacctg
     1921 attgtgcgga gacggcagcc gctttttgaa cactttctag tttagataat gacccatagt
     1981 tgtatgatac cgacgttaat gctgcttgtg cttgaggagt caatttagcc caaacgtcgg
     2041 caccaacatt accccttgca acattcataa attgaggcat tcttcgtgct aaatcacgtt
     2101 cagcatcttc ccggcttaca taagtgcctt tttgaacccg atgaaccata ccatctgcag
     2161 tagtataggt gtcagagcca tatccggcac ggttagcatt tacatcccat ttcccgtggc
     2221 ttaaatatcc ttcaaactgt ttgataagag ccgttgcttg ttcattcgca ttgccgttca
     2281 tttgggcaat ttgggtatag ctccctacat tcacgttact gacaatgttt gttggggagg
     2341 ctttatatgc tccataaaaa tcactcatgc ctgagccgtc tgatttgggg atagcaatat
     2401 atctatcgcc aacggcattt tgtgcagaat tagcaatgcc actggtgttg agattatttg
     2461 ctccggcttg attgcttaaa ttggcaaagc gacggtttcc atcacccatc gcattatcat
     2521 caatagcaag agcttgttcg ttcatctgtt gagtaattgc agctaatgct gtattaccgt
     2581 tgattgaacg ttgcagttcc atttcgagtt taacttgctc aatttctctg tcaagtaatt
     2641 tgaggtttct ttctgcttct tctactaagg aagattttat aacatttggc tcacgcatac
     2701 cggctaacat tgctcggcga atcagtagca atttttccat ttctttagaa atagctaatt
     2761 cttgcgataa ccttgcaccc agcaaggctg catccggcga agcagcaatg gcttctaata
     2821 cgccttttgt tacactgacc gttgatgtac tcagtgaatt gagttgttcc ggcgttaaac
     2881 tatctaagtg attcagtgcc gcttctagct ttgtggcttt ttcaatagtt gtttgttcaa
     2941 taaaatgggc gattcctttc ccggctttag ctccacttga actttgttca caagtttcac
     3001 aggcatcaag gaatgtttca ccaatcactt cacttgcata ggcagcagcc tcgcttggat
     3061 ttttccattc cgtacaaagc ataccatcac agcttgcaat aggtatatct cctttttcta
     3121 atggtcctcg tcctaaagcg aggttatatc cggctccaac aatgtcagca acgatagcca
     3181 ttggtttttg atttttacca ccacgttgtt cgccacctac ccaagggatg cctttttcag
     3241 catctttttt atccattttc ttcttggctg ccattgcatc aggctctgtt tcagcaattt
     3301 cagcggctcg gtgtaatccg gccatctttg ctaatttact gtccattgct aaatcagcaa
     3361 gttggttaga catggcttcg caactggttt tgaagttgtt gaagtcaagt tttccttgat
     3421 atagaccatt agaaagaatg tcatacagtt gaggatttgc tcgttggata atcatcgccg
     3481 ggaggctggc aactgcaccg gttgctgact caataatatt gccataaaga tctttaaaac
     3541 cttcagtaat accattaagc tggtttttaa tggaaagttt aatatcaaag ttgccacaag
     3601 ttaaattcga tttccagcca atgccaatcc catattgatt tggtctgcca cgggaagctg
     3661 gtgcggaaat ggcactacct cctccaatgg agtagtgcat ataatcagaa aggacagggt
     3721 agtattccgt gagagcgtta ttaccagaaa cgcctagatt accagtccta tagcctgttg
     3781 tattttcaat aaaattacta atgctattgc caacattaac ggtattacta tttgcgtatc
     3841 cgctcatggt tgtgagcatt atacaagcgg ttagaaattt gagacttttt gcttttttca
     3901 tttatttatc ctaatagtgc tgataattta atgagtatgt gtacttttcc agtccacatg
     3961 acctaaaaaa acttgccctt gttttttaca gcaagaatag ggcctccata atgcccagct
     4021 gtaattctca gtattggaga aactatctga atgggtagcc caatcaccac tgtgcggaaa
     4081 gactttaggg taggattcgg aagtaaatac cttaactgtt acatctgagg gtttattagc
     4141 aggactagca cttgccatag ctgaaagcag tagtagtggt aaatagtaaa atgtcttagc
     4201 ttgcataaat gctccacaaa tagagtaatt cacgtttacc ttaaaagatg atttggttag
     4261 aagaaatcgg aaattgtttt ttggttttgg agagtttttg ttagtatata agttaatgta
     4321 acaagggaga gtggatgtat gagcaataga atacctaaag cctgttatgc ttacccaaaa
     4381 tcgccgaaca acccgtttaa tagattgtcg gttatcaatc tataccagcc gaaatgtctc
     4441 ccagcattct gaaagggaac tctgtaaaat tacaggaatg ccacctaaac aataaatgga
     4501 taatgccagc cactttatcc gttgtatttt gtcacatgcg tgattgcaat gtcataatat
     4561 tacccgactt ggggatatta tgacattgca atcattgctc acttttctta gcctgagccc
     4621 acaggtgaat aaagggtgcc gtttgtgtcc tcgatgatgc cacagatatg ccgattaaag
     4681 accacaaggt aatcgggtaa attacgaacg aaaatatcaa cattgtcacg aaggtgctgc
     4741 tattttcaat atagtaactt atattggtct aacttaaatt tcatttattc ttcaaaagta
     4801 caacaccgtt ggtaagtgat ttatcaacgg tgttgtcatt tccaattctc taaaatcaaa
     4861 attcaattct caattaactt tatgattcaa tatggctaaa ttatcctcaa tttaagacgc
     4921 tgtatataac agcacttaat aagtcgcata actcctgaga agcgacggct atccttagag
     4981 gtagttgagt tattgccaaa atgtctccca gcattctgaa agggaactct gtaaatttgc
     5041 aggaatgcca actcaatccc tatcatttca gtgaatattc attgagattc cattttactt
     5101 ccaccttgcg gggaacattt ttcccgtgtt gggtaaggat gtttctccgc ttttttacat
     5161 ttggagaaac aaactatgac aactcaaact tctactcaaa aaaattactt caatctacat
     5221 actaccggca ttggctattt aaacgatatt cgtttagtag agcataaaaa aggaaatcct
     5281 ttttatgctt gtcgtgttgc agctcttgtt ggcgaatctg actcaccaga gtacagatat
     5341 tttgatatga atgtagtcgg taaagaaacc gaaacactca tcaaaaaatg ccaaaaagcg
     5401 gtctctgaag agaaaaaagt gcttgtatca tttgtaatgg ctgatttgtg gttcgatact
     5461 ttcacttatt caagtgacag taaatatcac aaaaaaggcg atactggagt gtctcttaaa
     5521 ggtcgcttaa tccgcatcaa aatgattaaa attgatgggg aaatcgtcta tcaagaaaaa
     5581 ccaaaagcag atgagcaatc tgagtaattt tacccaagcc cgacaattta tgttgggctt
     5641 tactttatgt cccgtaggga aacactccct gcggtgtgtt ttcctttatt ttacaatttt
     5701 aggagaaaca cgatgaactc acttcaacaa tttgtcaaaa cattatcacc aacagtcctt
     5761 aatggtcgcc attttgatgg tattgttggc gatgttgtct ttgttcagaa cggctatggt
     5821 aaatggcttg atcaaggctt tactattatt ggttttgaca aaagcataga ggaaaaggat
     5881 tggatttacc tagattggga ttgctattgg ttcggaaaaa atccggcaca ggtaatccct
     5941 gaaaaatctt gggctcaacg ttcttggctt gctagagcgt attcaatctc tcttgaacgt
     6001 tttaattcgt taggcttaga gcgtgaatat aacattgtgt ctgattggga taaccccatt
     6061 tatggtacag atattcagtt aattgaacat tccaaggagt ttttccgttg gactgaagcc
     6121 gatgatagag ggattttctt agttcacaac gagatggctt ttcatttctt caaaaaagaa
     6181 attaaagcct catcggaacc ggaatactat caacctattg agacattctg gaaagatgct
     6241 acggtggtat tgaacataga agatctagat atgttagagc gagtaggatt agagcaaact
     6301 aatacacctg ctttagcaaa attaactacg tttgtacagg ctgctcgcaa cgaaatttcc
     6361 aatggtaata tcgtattcta tcgagcattt taacatctaa tacaaagagg attattatat
     6421 gaatcgacaa ggtatttata tcaacggtaa acaagtcttt gactctaaag gcaaagagat
     6481 caacagcgtt caaatcatta atagtacagt gcaaacagtt gttgggataa ataaaggtta
     6541 tatctcaatg gctgccggca ataatgtatt aattgttgat ggacaaacaa ttgcattaga
     6601 accctctccg actatcaaca ttgaagtatt tggcaatgta accaacctca atgcaggttc
     6661 agaaaatgtt cgtgtaacag gcgatgccaa atctgtttca actgtcagtg gtgatgtcac
     6721 agtggatggt aatgtaacct cgaatgtgaa aactgtgagt gctgacattc attgtaaatc
     6781 tattttaggt aaaattaaca cagtgagcgg tgatatttat cgctaatatt gactatacac
     6841 cctatcggga aacattctcg gtaggtatgt ttccccactt tttatttaaa acttggagaa
     6901 acataatgga agattccttt tttgactttg atgatatctc ttgctattta ggacaatggg
     6961 aagccatctt agaagaatat agcgatattg tcagtattga agacttttgg cttgttgcca
     7021 aagaatttga aactgtacca catttcggta atctgtatca ggagcttgtc atctctcgct
     7081 taattcaacg attctgtact gaattagata ttgagcaaga ttcagattta gttgaatttg
     7141 actactatat caatgcaata gacacgcatt tttacattaa tcgccaaaga atctgtgata
     7201 tagatgattg gaatgaaatg ttagacaaga ttcgtaaaga aatgacgccg gctaaattag
     7261 ctgcctaatt ttttgcaaat ttttactaaa aacgactctt gccgcagtct tagtccaccc
     7321 acttggggaa atactcccct aatgggtgac atttccccgt attttattta ggagaaatgt
     7381 atgacgtttg aacttggtcg agtatgtaca actcgtgctg ttgctgaatg tttcaatcaa
     7441 aatgccttgg ttgaattgct taatcgttac tgttatggtg attggggcaa tttgtgtgag
     7501 gaagataaat tagcgaatca ggaagcaatg aaatacggag ggagaattgt agctcgctac
     7561 gatgtagatg atgaaagtct ttatatcatc acagaagctg atcgtagctg tacaacaatc
     7621 ttattttctt atgaatacta aggagaaatc cgattaacac agcacattat tgtgttttca
     7681 aacgccgtct aatgatggcg tttttttatt ctctcaatca acaacagaat aaatcattca
     7741 aaatcagcaa tgacttcgcc atagttggtc tagattcctt tcatacttgg agagattaaa
     7801 tgaaaacaaa aatatcctta atgactgttt ttatgcttgg cttaagtggt tgtgttcatc
     7861 aacaggatac agctcaaaat agtgatgaaa tcacacctat gcctgattca ttgctaacac
     7921 ctcaaatgca acaattacct ccacaagacc gtatcgtgtc caaagatatt taccaagaac
     7981 agactacctc gaataccgaa gtggtacgtg acggacgcta caccttaatc agtattgctc
     8041 cggaggaagg tcaaaaatat ctgcttgaac aagtcgtatc agtcaaagtc tccaatccta
     8101 aaaatcgttt tactgcgaat gtccagcaag gtttgcgttc tacattaact aatacgggtt
     8161 tagatttgtg ttatccacct atcggagaac aaggtaatgc ggttcaaaca ctttttaccc
     8221 gacctcttcc aaaagttcat taccaattcg gtccgatgaa attatatgaa gctctacaaa
     8281 tgattgcagg tcccgcttat gaactaactc ttaatgatat atcccgtacg atttgcttta
     8341 aagctcggga aagttcattc caaaaccctc ataaacctgc tgttgccgtt acaacgacaa
     8401 caattaaaac cgaggtcatt gaggaataat gagggaaata tcaatgaagt ttaatttatg
     8461 ccgtaaagct ataccgttgg cagtactacc tcttttattg tcctttgcat tacctactct
     8521 ggctcaaaat atgcaaacag agaccgtaag tagctcacaa tcgactttgc agaaacaaag
     8581 ccaacatcaa caatcacaat taagtgatag cctaaaacag gctaaagaat ggggattgac
     8641 cgttgaagaa tggagtcgtt ataccgaatt aatgaaaggt gagcgaggta tgtggtcgcc
     8701 aaatctagat ccgttaactg ctttaggtat cgaagcacga actgctgagg aaagggaaaa
     8761 atatgctcgt atgttggcga aaaaatatta tgaacgagtg tcaaaagaac ttgattttga
     8821 caaagtttat cgtcaggagt ttgagaaact ttatccgaat gagctacctt ttgaggttga
     8881 accacatatt tctcaatcgg taggtcgtgt ggtttatttt acccgttttg ataactgcga
     8941 ttcttgtaaa agtgatcttg ggaaaatcct ctcttacgca aataatcaaa cacctctcga
     9001 tatttatatt gttgggcaaa acatctccga taatgatatt cgtaaatggg ctgccgacaa
     9061 taatattgac ccaataaaag tcagtaaaag attgattacc cttaaccacg ataaaggcta
     9121 ttggctgaga tattcgttag gaaaaatgcc cgctgcttac caaatcaaac aggatggtga
     9181 atggacatca ctcgtgtatt agtcattgtt gcagtctttc tttccaacat tagcatttct
     9241 caggcaaagc ccttcaatgg atttggttct gaaattgaca gctatattcg tatggctgct
     9301 caacgttatc aggtgagtga agcaatgcta cgaggttttg tcaaaatgga agatggctgg
     9361 tatggcaaca tttcaccaac aggagcaacc ggtgttgggc agtttactat gggaacctgg
     9421 aactggctat ctgaaacaga gaggggaaag caacttggta tgaagttgat tacagccaaa
     9481 aatcggggtt cttacgccga tccccgtcga gataaatata cgaatacctt agcaacggca
     9541 ttattggcac gttggcatat tgagcaattt caactccgta atatccctat tactgatgag
     9601 aatttatata tggctcataa tatcggctta gatgggctgc atcgagcaat gatggggaaa
     9661 gctactaaag aagacatttt aaatatgcgt cgtaatggaa tgaaaagttg gatgacggta
     9721 agagactttc tcaattacca aaaaggacgt tttttagcac ataagcaaga agccaatttt
     9781 atgactgtct ccaagcaagg gcatattcat accggagttg cagctttcgt ttatcccaca
     9841 agccaaatgg gaaaatcggt caaatgggtt gagccggata ataaaatgat ttgggttgaa
     9901 cctaaaaatg caatggtttg ggtagaaccc caaaatactc ttcagaggta aaaatgcaaa
     9961 acaaggtaaa agtattattg tttagtttat atgtgtccca tttctcttgg gctgatttaa
    10021 ccgtgattgc tgatttcggt ggtgaaagtg cggtacgttt ttatgaatct ttgcaaccgg
    10081 aacacgatga aaatgcacca aactatccga attccatttc agcctcagtg agtgaggctg
    10141 atattttacc cattgtttcc cataaactat ctcccgggac tattaaagaa aaaccatttg
    10201 atttaaccgg aatgcaagcc attttcttaa ttggagcaga taatacctca gctcagtggt
    10261 tacaacaaaa tcgagagaag ttgatcacgt tgaatgcaac agggcttgta gtgaatgtac
    10321 aaacactctc cgaacttaat caattacgag acattgtacc ggaactgaga atattaccaa
    10381 ctcccggaga tgaccttgca gagcgaattg gtattgcaca ttaccctgtc ttaatcactg
    10441 aaagcggagt ttcacaataa tgtctgctaa acatacctta gaggcgtggt tacgacctcc
    10501 tgttgaattt tttccggcag cggttcacgc ttcttgtgcc tttgtcagtg ctgcagctcc
    10561 ttggtctttg gcactccatc caagtatcgg ttacagtatt gcagctggtt ttggagcatt
    10621 gagcttttat cgggcaaaac aaggttggca aattgtgcgt taccatcgca acctgaaacg
    10681 tttgcctaat tattcaatga caagcaagca aattccggtc agtaataagt atctgtttgt
    10741 cggtaaggga tttgaatgga aacctattca cactcaacgg ttacacgatt gtttttcgga
    10801 taacggttca aaatatctgg agccttctaa gctatttaaa atggctcgtg agtatgaaaa
    10861 acgacacgaa aattggctaa cacatcttac ctcaaaggat agccggtgga atctggttag
    10921 acctctgcca cctgttgagg gtattcctgc tatccatgga attgagccgg atgaggttga
    10981 tgtcgttcaa tccgttagct ctcgggttgg gcatacggta gtattcggta caacacgtgt
    11041 cggtaaaact cgtttagctg aggtctttgt tacccaggat atccaccgag gtaaaactgc
    11101 tgatgagcgt gaagtggtag tcttctttga tccgaaaggc gatgccgata tgctcaaacg
    11161 aatgtatgcg gaggcaaaac gagcggggcg agaagatgag ttttatgttt tccatctagg
    11221 ttatcccgaa atttctgccc gttataaccc agtaggacgc tttaatcgta tttcggaagt
    11281 cgctgggcgt atttcaggac agctaagtgg agcgggtaac tcggcggcat ttaaagagtt
    11341 cgcttggcga tttgtcaata tcgtcgcacg tgcattagtt gaattgggaa atcgcccaaa
    11401 ctatgttcaa atttcccagt ttgtacaaaa tattgattca ttattccttg attatgcgga
    11461 aaaattcatt cgtaaaaccg atccaaaaat ttgggaaaat ttagcaacca tcgccgctgg
    11521 actggatgaa aaagcacttc ctttcggaat gaaagaccgt ccgctgattt ttgtgatcaa
    11581 caagtacatt gctgaaagtg cagtctttga cccaattcta caaggacttg ccagtgctgt
    11641 acgctatgac cgtacctatt tcgataaaat tgttgcctca ttgttgccgt tattggaaaa
    11701 attaacgaca gggaaaattt ctgagctgtt atccccggat tatgctgata ttttggatga
    11761 acgtccgatt tttgactggg aatccattat tcgtaaacga ggaatcgttt atattggttt
    11821 agatgctctc tctgatgcga cagttgcggc cgctgtagga aacagtatgt ttgctgactt
    11881 agtctcaatg gcaggtcatt tatataaatt tgggacaaat gacggattac cgggagcatt
    11941 caaagaaaaa tccaaaccag tgaaaattaa tcttcattgt gatgaattta atgaattgat
    12001 gggtgatgaa tttatccctc tcatcaacaa aggcggtggt gctggaatac aagtaactgc
    12061 ttatacacaa acactctccg acattgaagc tcgtatcggc agtaaaccga aagcgggaca
    12121 aaccttaggt aacttcaata cactctatat gctgagggta aaagaaccgg ctacggcaga
    12181 attactcaca aaacagttac acgaagtgac gattaaacgt agcacggtca cgtccagtac
    12241 aactgactcc agtaatcctg acgatgataa ggcgtttagc tccaataccg gacagcgtat
    12301 cagcgaaagt accgttccta tgttggttcc ggccaacatt accaatttac caaaaggaca
    12361 agcattcgct ttaatggacg gtagtaaatt atggaaaatt cgaatgccgt tacctgctgt
    12421 cgataaagat gatatgatgc atcaatcttt acgagagctg acagattata tgcgtaaaaa
    12481 ctatcacgtt gttgaaaatt ggtggcaacc ggcatttaca gaatatacgc cgtcccgaga
    12541 cattgcagaa gcatttgaag cgatgtcgga gcctcaatat aaaacacaga tagaccatca
    12601 agtagataat ttcggcatag atgaacctta cgatattgat gaagaggaaa atgatgagta
    12661 gtgccaaaat gagagaggaa aaaagaacca atctattaga tttaccaaat aaataccgta
    12721 attttaatgg ggagttttca gcttcctgtg gcttagataa tgcagaagaa ctcctgattc
    12781 atagtcaatc ctattttatt gaatggtttg aacaaggtta ttccttccat cagtttgccg
    12841 aaaaattcgc agatcaaggg ctttcgttat ggtctgccga tgaagtatct atgcgtcact
    12901 cggataaatc caaagatatt ttcgcttttt atcttgcttt tgataataat ccttccggct
    12961 atatccttgt ccagtgccag ctcgatagag aggatagttt gcaatgagtg aagaaactaa
    13021 agctcgccga aaatcaggtt taatcaacct gatttttgga aaaataaata tgtcgcttgc
    13081 tgcaattgca gtatctctca tattcagcat tattttagag tggataggca ttgctttctg
    13141 gtggccgcaa gaagggcatt tgcatagcca aacgatgatg ctcaatgaga tgcaatggct
    13201 atcggatgac ttttctcaaa gcctgtttgc caagtcttct actgcttttg caacatcaat
    13261 tattgaaaac gtgtataact ggttattcgt ttatacgggt gttaaggagt ggttaacttc
    13321 accgggtaat ggtagtatgg aacagtgggt acatcactac ggcagagcct atattgaatc
    13381 tgttatttat gtggtgatca cctttattat tcgcctcatt attattgttt ttaccagccc
    13441 actcttttta ttgactgcac tttccggttt aaccgaagga ctgtcattac gagatatacg
    13501 aaaatttggt gccgggcggg aatcagccta cctataccat cacgctagac gttatattat
    13561 gccaatgatg gtcacagctt gggtgcttta tttatctatc ccgttttcaa ttcatccgaa
    13621 catcatttta gtcccagcag cctgtttgtt tggtttatcc atctgtataa ctgcggcgag
    13681 tttcaagaaa ttcttgtaaa tttcataagc ccttagcaat atgcgggggc ttattcttct
    13741 gttgttgcct ctgtatcttc attttctgac tcagtatccc aatcagaatc ctcccagtct
    13801 tcgacttccc aatattgtgg atattcatca tcttcaatga tttgttcaga aatttgttca
    13861 gaggtattcc aaataggaaa ttccccgtac tcatccaatt gtaactctat cttttttcgc
    13921 tcatcatcgc ctagaaattg ccaataatta cagattaact ttcctcttaa acttaattcc
    13981 caaagacctc cgtaattata ttgagcattt tctgcatggc aaatattccc cactttctgt
    14041 gctgaaggaa gagaatgggc aacatatggg aaaactacaa ggaacagtgc tagatatctc
    14101 atctggtatc tcatcgtaaa gcgtgaagat ttcggtgttg atactactta attgactagc
    14161 ttaaacatag aataatatct ttctcaccat ttactatttt gtgcaatttc tcttctgaac
    14221 ggtaacagag ttttccatta aattttagga tcgcttttgg cactcttaac ctgaagtaaa
    14281 actaaaaata gaggtgcact atgtctattt caacatcttt aaggaaaggt attgttgtcc
    14341 ttgttacctt atcttgcttg ccagcacttg cttcaacaga acaagagcaa ttagcccagg
    14401 caattaagca actggaagcg gcaaaattat cacttcaacg agcagaaaaa gtagctaaag
    14461 cctcgcctaa aacacgagaa tactttaact attctgctat tcacagtgat attgaaaccg
    14521 tcaaaaaagg aattcaacaa tatatcaatc caagccgagc attacctcgt gatccgaatg
    14581 tactacgtac attaagtgat gattatagca aattacgggg gcagtgatgt cttactcccc
    14641 tgcgaatgcg ttttctgttg ccagcggtat cgaccctttt agattgaaaa tcatgatcgc
    14701 cattattttt ctttcattac tgctcttagc cgtcgcttgg gcattgaata gtggttataa
    14761 gggattttca tttagagata aaagtccttt ttcactttta gttttatttc tcaaaggagt
    14821 ggcactgtta gcttgtgtta cttatttctt tatctattag attcgaggtc tattatgaac
    14881 cgcattaaat ctcttacgaa acatctgcat agtcgttatt tggctgtaat gctcttcttc
    14941 tggggttccc tccaaaatgc atttgcagcc ggtcctgatg cttccaaaat cccagacttc
    15001 caaattccag gcgtggatag taataccaaa gatccaatgg aaattatttg gattgttctg
    15061 aaaggtcttg tcactctcgg agctgttatt ttagcagccg tagccatttt gacagttgtt
    15121 aaagcattga ttggcatcta taacgaagct acatctgaaa atggtaaaaa aggttgggga
    15181 cactttattc tcgccttaat tatcggcttt attgtgattt tcttctgctt gtggttagtc
    15241 aaacttgcag tcggactctt ctaattaaaa tgtctaggag accaatatgt cagaacaaga
    15301 tacaaccatt ccttttcttc ccacccgcct taatcgtgaa gctaccgttt acggtgggat
    15361 gacagttagt gagttcggca tatctgccgg attaggattt atgttaggtc tgattgttgg
    15421 tctcttcttg tggatattaa ctgatttttg gcttcttatt ccggcaatgg caatgttgct
    15481 ctgcatcgca actgtcttaa tcggaaaagg tattgtcgct gcggttaaac gaggaaaacc
    15541 agaggcttat ttaaatagat tagttgaaaa gaaaatggat gacctcttta acggtcataa
    15601 gtttataaaa cgagaagggt tttggtctat tcgccgatat agaaggaaat aacaaatgag
    15661 ccagttaaaa agccaattac acgaaaaaga taaacatatc aaaacgactc gaatttttgc
    15721 cttcctgttt ttcattatta ttttggtact ggcaggcgta atctatactt tcccaagtcg
    15781 ccttacgata tacaacccac cagatctgcg tgctgggagc caacgagctt ggtgggaaat
    15841 tcctaaacca acaatttatg cattctccta tcaaattttc cagcaaatga atcggtggat
    15901 gacaaatggc gaagaggatt atgagaaaaa tatcaccacc ctcaaaccat ttttaacacc
    15961 acactgtgaa gcatttttac gacaagatta taaagatcgt ctgcgtaaca atgaactacg
    16021 cagccgaacc cgtatggtgt atgaaattac cggtagagga tatagtgaag ataaagttac
    16081 catattcagt gaagatagtt gggctgtcaa tcttgattta agtgtggatg aacatttcca
    16141 aggacagccg gtaaaacgag tttatattcg ttttcctatc agtattgtga gaatggatgt
    16201 cgattctgct caaaaccctt ggggattagg gtttaattgc tatcactcca ttccaaaact
    16261 cttagaaggt gttgagccaa aaacagagga gaaaaaataa tgaaacgtct accaattttg
    16321 ctcgtctctt tgcttatagg cttctctggg agtagcttgg ctgatgtatt gatgaaatgg
    16381 gaaagaaaac cacttcctgt tgcgttacaa gtggaaaaag aacgaattat ttttgtcgat
    16441 aaaaatgtca aggttggtta tccggcagaa ctggacggta agctacgaat tcaatctacc
    16501 ggtggagccg tctatttaaa agcattagaa acatttccct ccactcgcat agaattccgt
    16561 gatgttacaa ccggtgaaat ttttctattt gatttaacag cagcttcgaa agtcgctaat
    16621 cctgaagagg caattcgagt ggtttttgac ggtacggtag aaaaacaatc tttttcaagc
    16681 aatagagatg atgaaagctc tgatctgcct attgcgaata gccgtcctca gcttccagta
    16741 cctgccgcat tgacccgtta tgcagcacaa tcactttatg ctccactacg tacagttgaa
    16801 cctttagatg gtgtgcgtcg tgtggcacat cgtctaccta aaaaaattac caagctgcta
    16861 ccggcttatt caatagaagc aacaccttta attagctggc agttagacga atatgtagtt
    16921 accgctattc gtttgcaaaa taagggtaac actcaaatta cgcttgaccc tcgagagctt
    16981 caaggacaat tttatgcagc gactttccag cataattggc ttggggggta cggagaaaat
    17041 gaagatacca caaccctgta tttagtgacc gaaggctatg ctaataacgc attaattccg
    17101 gaaacccgtc cagtcaaact agtcagtaaa aaagtgaaaa gaacaacaaa agttgaaact
    17161 aaaccggctc aatcggcgaa gtaggaggaa aggcaatgaa agcgaataaa gccttatttg
    17221 tcatactggg cattgtattt atcttagtcg gtggaattgt ctatgcctta tttggacgta
    17281 gtgagtcagt tccacaaccg acacaacagg aaattttaga tatttccatt aaagatttaa
    17341 cacctgaaca agtcagaaca atgggtattc aaggtgatac gccgattgat acgctgcgta
    17401 cccttgtgac aaccgttaaa tccaatgagc gtcgtatgga tgagttaatt gatgaaaatc
    17461 gccaactcaa acagaaagaa gatttagtga atggtcgtat caatgatgcg gtaaataatg
    17521 aaacccgaaa actgcaagag cagattgcag atttgaaaat gcaattattt gatatggcga
    17581 ctctcgttaa taacaaacca acaacacaag agtctacccc tcaggtagag gctcagccta
    17641 atacggtacc gaataaagga tttggaacag caggagataa attacctatc ggtggtgcaa
    17701 ctatgtccaa cgtggatacc agtggaatgt attgggtaga accaagcgat cagattagtt
    17761 tggatgcaag tggaaaacct gtgtcaggca atacagtagc aactaaattt ggctttccga
    17821 aagattttaa tggaggacaa tctgcaggaa ataacgaaaa gaaaggtctg ccgtttttcc
    17881 cggaaacaga tactgtgaca agctcacaag gaacggctgg aaacgcacgt ggtaaaaacc
    17941 agatgacatc agtatatacc ataccaagta attcaacatt ccttgattca gttgcaatga
    18001 cgggactcat cggacgtatc ccgattgata atcgagtatc ggatgcttat ccgttcaaag
    18061 tacttattgg tcgagaaaat ctcattgcta atggtattga attacctgat gtagacggag
    18121 caattgtatc aggtactgct actggcgact ggacactatc ctgtgttaga ggcagtgtaa
    18181 aaagtatgac ttttgtgttc aatgatggac gtattgtgag tacctctaat aaagggaatg
    18241 gcgaagagat tggctggtta tcagatagtt cgggtatccc ttgtgttcca ggagagcgga
    18301 aaaccaatgc cccagaatat ttgggaacga atttcttttt ggctgctgca agtgctgctg
    18361 cccaaggatt ctcacagtca caaacaacca ctgttgttga tggaagtagt gttgttggag
    18421 ctgttaccgg cgatcagggt aagttcatct taggacaagc tatcggaggc ggcttaaaag
    18481 aatctgctga ctggtttaga aaacgttttg ctgaaaactt tgatgctgta tatgtgccac
    18541 cgggtcatcg tgtctctatt catatcgaga aagagattga aatcgattac aacgcccaag
    18601 gtcgtaaagt gaaatatggc tcaaaatcaa cgaattcaca actggattag aaggaacaaa
    18661 cgatgaaaca tttattttgc ataatgataa gtgccttatt gataacaggt tgcacaacat
    18721 caacagaaga attattacct actaacggca gtacgatgcg ggatatttgg gataaaggag
    18781 cgagtaataa cgatctacac ttataccgtt cacaacaagg gcgaacactt gaccctgtcg
    18841 attatattcc taatagtgag caacgagcct atacccgtac ggcagaaaat gaaattaata
    18901 acttgttccc tcgtttacct aatccggatc tgattatgta tgtgtaccca catcaaactc
    18961 gttccggaga gcctctcagt attccaggat attctacagt actacctttc tatggacgag
    19021 ttcaatatgc tcagccgggt gatagaacaa gagggcttta atcatgtctt ttttaagtga
    19081 ggtattttcc tctttcaaac cgactaaagc aggggttata tcaagtgaac caaatgaaaa
    19141 taccggtgag tctgacatca ttcaaaataa aatgagtgag gtaaataacg aggttcaaca
    19201 attaaaacgc agtggaaaac tcacccagtc acagcatcac cgtcggttat actcacaaga
    19261 gccctccttt atcgatcatc taccttgggg agaatatttg aatgagcacg gtgtgatact
    19321 ccttgatgat gcccgtagtg ttggggcagt atttgatatt aagccgattg gtactgctgg
    19381 ccgtagtatt gaatttttaa gtcggatacg taatcttgtc aaagatgcct tgcaagacag
    19441 ctttgatgaa ttggatgtaa atccgtacgt tgttcaattt tattgccaag acagcgatga
    19501 tttacagcct tatattgaac agctacgaaa ttatatctcg cctatcgcta aaaatagtgc
    19561 atttagcgaa gaatggttac gggtacaagc caagcatttc aaggatatcg gtaaaccaga
    19621 ggggcttttt attgatgagc gagtgacaaa tacagcttgg tctggtcgta tacgtagtac
    19681 caaaatggtg atctatcggt atgttgataa atccaatgac actaatgaaa atctgattgt
    19741 cgagcagctg aacaatgctt gttctcgcat aaatggagct ctaacaactg ctggattaca
    19801 attgcagcga ctggatggcg aacaatttcg tcaatggctg ttacgttggt ttaatcccaa
    19861 tccttctatt aattttgagg ggttaagcaa caatgattgc taccatcttt ttggtaaaca
    19921 aacctcaaat gacgggcttc cagaattagc gtttaacgat ttcagtgaag atttatttta
    19981 ttctcaaccg aaaaatgaag gcaactattg gtattttgat gaccaaccgc acgaagttgt
    20041 tgtagtcaat aatttgagga aacaaccgga agtggggttg atgactggcg agattactcg
    20101 tgggcgtaat atcaatgcat tatttgactt gttgccggaa tcaaccatta tggcaattac
    20161 catcgtcatc catccacagg ataagttgga ggcacattta gaaaaaatta gtagtcgagc
    20221 ggtaggtgaa aattttgagt cgaaatacct aaaagaagac tgtcaaactg tacagtctta
    20281 tcttaaagag gggcataaac tctatcaagc agctattgca ttttacgttc gaggtaagga
    20341 tgaaaaggag ctacgtaccc gttctcgcca gttgagaacg attctgctca gtaacaacct
    20401 agaacccgtg aaggaaaatg cagaaattgc cccacttaac agctatttgc gttggctacc
    20461 aatgaatttt aatccgcaga tggatgccaa atcacattac tacacgaagt actattttgt
    20521 acaacatttg gcaaacttat tacctatctt tggccgagat accgggactg ggaatccggg
    20581 gatcacttac tttaatcgag gaggagcacc atttaatttc gacccattta accgtaaaga
    20641 cagaaccaaa aatgctcaca agctattatt aggtccaacc ggttcgggaa aatcagctac
    20701 gttatgtggg caattatgtc aggctatggc cgtttatcgc ccacggactt ttgttgttga
    20761 ggcaggaaat tcatttggtt tattagcgga ctatgcagaa cgtttcggac tgactgtaaa
    20821 tcgcattacg ttagctccaa acagcggcat tgtcttacca ctcttttcag aagcacatcg
    20881 tctattggat atggaattaa acccggaaga tgtgccggaa gacattgagg ataacgatga
    20941 cactgatgat gaaggtgata tgcaacgaga tttattggcc gaaatggagc tgacggcacg
    21001 tttgatgatc accggaggtg aaaagaaaga agatgaaaaa atgagccgtg ctgatcgtgc
    21061 tgctattcgt cgtgccattt taaatgcagc tcgccaaacc aaatctgaag gcagacaaac
    21121 gctgactgaa gatattaaaa atgctttgga agcattaagt catgatgagc atactcgtga
    21181 agaacggcgt acccgtttag cagaaatggc agaatcatta gaaatgtttt gtaccggtgt
    21241 aaatgggcaa ttttttaatc gagagggggc aatttggccg gaggctgatc taaccttagt
    21301 tgatttagct atgtttgccc gggaaggcta tgaagcagag ttggccattt cctatatttc
    21361 actcattaac catatcaaca gtcttggtga aaaataccaa cacagtgatc ggatgattct
    21421 gaatgtaaca gatgaatcac acattatcac ggccaaccca ttattagcta aattcctagt
    21481 taaaggctca aaaatgtggc gtaaactagg gatttggcta tggcttgcta ctcaaaatat
    21541 ggatgacttc ccgaatgaag cggcgaaatt actctcaatg gtcgaatggt gggaattgtt
    21601 gaatgtgtct gccagtgaaa ttgatgaaat caatcgtttc cggaaactgt cagaggccca
    21661 gaaaacgatg ttgttatctg ccaaaaaatc agatcgtaaa tataccgagg gcgtggtatt
    21721 agcaacgaat atggaagctc tattccgagt tgtaccgcct agcttatttt tagctttagg
    21781 aatgactgaa aaacatgaaa aagcagaacg taagcaactg atgatgcagc acggctatac
    21841 cgaactcgat gcagctttag aggttgcagc tcggattgat aaagcaagag gaatacacta
    21901 atgaataaac aacattaccg tttatttaat gcaggcccac aaaaatcctc attaggagtt
    21961 gtatctccag cggtagaaac tcaattgctt ggattttgtg aacaaattcg ccaactcatc
    22021 ggcgttggga aacagattac cttattccct catcgtgtcg tgcaaaatca ctgggctatc
    22081 gaactttctg gtgagcaggg ttctgttcac ttattactta atgtgacggg gcgttctttg
    22141 ctaccaactt ccaataacag tgaatggtca gctcgtttaa ttattgaaat agctgatcat
    22201 gtggatgctt attggttttc ggcattcctt actcagtatc tcggtttgaa aagtaatctt
    22261 ttacccttat ccacatcaat tgcggataac tctgtgtata acgaggtaat agataataat
    22321 gaagtctgat acaacaaagc ctaaaatgac aagggttatt actgtattaa aaataacctt
    22381 gatgatcatc ggtattattt tattactact cgttgttggc tggttggcaa tgacctattt
    22441 tccttccaca aattttcaag cgtggttcta tgaaacccgc tatgcctggt tgggatggcg
    22501 atttgtttta tatgtagcta tttggctatt gatggtacaa cttaatcgaa aagggcaaat
    22561 gccattaaaa gccaggttaa tgattttatc tgtcatccca attattgagg gacttaattt
    22621 actgtatcta ctttaaggag gatgagatga cctttaccgt agatagttac ttagaatact
    22681 ttcttacctt actggcttgg atcatcaaca acaatatttt cgctgtattg gttcaaacag
    22741 ggttgtttgt tctgcctctc atttttgtct taatcagtac gtggatggaa gtcaaaaaac
    22801 aaggtgagga cgaaggtaat aaaggggatt tacttattac ttggctcagc ctcaaatttt
    22861 acccggcgat gtttgtcgtt gtattggtct tagctccaat gataccgatc aacctgaata
    22921 atattgagtt gaatgtagaa cgttctaaag cctgtggtta tcgtgttcca actgcaccag
    22981 aggatagtgg ttataaccaa atgaactctg aattgggtga tcaacaagcg agagtcccta
    23041 tctggtgggg attatttcat caaatcaaca aaggggtaac acacgcctta acagcttcaa
    23101 ttccctgcaa accggattta cgtcaagttc gttttgaagt tcaacacgag aaaatcaata
    23161 atccagcatt attgacagag gttcgcttat ttgtacaaca atgctatgta ccggcacgtc
    23221 agaaaattca aaatagccaa ctttcattga gcccagcaca ggttcgtgag actagctggt
    23281 taggtggccg tattttgatt gcaaatagcg agctttatcc ccgttatcga gcccaacaac
    23341 ctcatagtct atttccctat aatgcttctc gggataatgg attgccgaat aatggagagg
    23401 gtggttttcc aaattgtgca gagtggtggg atacggcgaa taatggttta aaagatcgtt
    23461 tattagatga tgtaaaacag aacttctctg caaaagtgat tgattttttc agcagtaaag
    23521 aaaattccga tgaatccata cttcgtaccg tattacgagc tgagaatatg gatatttcga
    23581 gagggaaagt ttatcccggg tatgggggaa atttagacag atcatttaca aatgaagtga
    23641 ctaaaacagc ttcaatgttt ggtctgtcat taggcagtat cggagcattc cctgcttttg
    23701 attcgatgcg gcaagcatta ccgatggtgc attcatttgc attaatggca ttgattattc
    23761 tcacgccttt aattatggtt atgagtggtt attcgttaaa aacattggtt acgctaactt
    23821 ttgtgaattt cgcattagtt acactcacat tttggtggga actagctaga tggcttgact
    23881 cttggttgct gtcggtcctc tatgattcag gcacacatag cagaataaac ccttatttcc
    23941 ttgaaaatac acaagacgat attattgtga attttgtgat gggttctatg ttcttggtct
    24001 taccagcgat ttggctaggc agtatgtctt gggtaggagt aaaaatggga gagtttgcaa
    24061 gtcatttaac tactggttct agagactcta aaacagcggg ttccaagata gggaaataat
    24121 ctaacaactg aataatagag tggtatatac cactctattt acttttactc atcaacacgg
    24181 taattaccta gatatatacc tggtccatca tacccatatt gatattcatc atcttcttgt
    24241 tcttcatcat cagaaaaaag tgattttaaa tctgtatctt tagacgatat ttctgaatta
    24301 gccatatctt tatattc
//
LOCUS       DANLZC010000019        24165 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.019, whole genome shotgun sequence.
ACCESSION   DANLZC010000019 DANLZC010000000
VERSION     DANLZC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 24165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24165
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.019"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(2..1114)
                     /locus_tag="PXE44_000601"
     CDS             complement(2..1114)
                     /locus_tag="PXE44_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588142.1"
                     /translation="MKNNKTDTLLFLEQFARYLTAKSWQIAFELEYAVIWQKDLGHRI
                     LEVTLPKPIAEDREEVLQKALEKLAKIEEKNVETLKLNISNQYTDKLSVRVIGESVKD
                     GTIPLNEGVKLFEKTKHLINALALSAKKKKAAFGHNSGGKDVAEFMSQVRLGQTQIGS
                     YIVNLSYPVENIEIKEQNEIIQAISFSRSVSHNLVNSLNKLKEKITHYDHNPTIFAEL
                     IPEGVSHNLCEAIIGLSGSDQQRKIEIKLQVGEISDESMPSETFNIKFSQKEIKVIQV
                     ASRYHQGEYTLPHYEIIGKVVGLHSSNLADGGYIQVPCKIEDKTVDIRVDLPPEQYKL
                     AAEAHKTEQQIRCKGENLYINKKKGRLQKLTSINIL"
     gene            complement(1111..1614)
                     /locus_tag="PXE44_000602"
     CDS             complement(1111..1614)
                     /locus_tag="PXE44_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HDL5588143.1"
                     /translation="MLSPTQQMEQFNIAYVLAITANAGFNHAVPVVDNHSVDLAISAE
                     FPTEEGKRSDPEIKLQLKSEGDLHIKNGLVSYKLKKKNYDDLRKNCANPRYLIVCDLP
                     KKTTQWLSHKKKFMTLKRHCYWVSLKGFPATTNRSSVTLTFPENQRFTTDVLIKMIEY
                     ARVGETL"
     gene            complement(1619..2350)
                     /locus_tag="PXE44_000603"
     CDS             complement(1619..2350)
                     /locus_tag="PXE44_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5588144.1"
                     /translation="MRPLKEIRYDNLLRLIDEAKSTSDLANRTGIAVSYLLQIKNKNA
                     IQNGKPKGIGDKIAAKLEDGMNKPRGWLDQIHSEQSNLINSRIGNEEQKLQEQDLNDF
                     IIIDVLDVSASAGFGSSSELVEVVNQMRYVPEQFYSLFRNMAPRYIRIINLSGDSMYP
                     TFSSGDMLFVDISVNEFTGDGVYVFTYKGHLYVKRLQNTGDQILVISDNKLYEKWSIT
                     EENQDQLFIHARVKVHQSQQLNFIG"
     gene            2477..2701
                     /locus_tag="PXE44_000604"
     CDS             2477..2701
                     /locus_tag="PXE44_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5588145.1"
                     /translation="MQLQEYIEKSKWGEISRLAKAIDVAIPIVSFWVNGKRQVPAKRC
                     PEIEKFTEGKVTCEELRPDVDWAVLRNSGK"
     gene            2750..3511
                     /locus_tag="PXE44_000605"
     CDS             2750..3511
                     /locus_tag="PXE44_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL5588146.1"
                     /translation="MKSQSLLPITAQNASGAEVKISSIEIAELCEKQHKNVIVDIRKM
                     LAELKLNSADFSAQYQTKDGRMQPCYFLPKRETMILVSGYRIDLRAKIIDRLDELEKQ
                     HQPSIPKNYPEALRMAAELAEQNQVLLLEKQQTTAENHALKSYFEPGLTPAQFVKGLN
                     GVNSNKINDYLRSRGWLYKDKHCWRVFSHVRDKYLTESFKRIEVDPVERIEMATYKPI
                     LLEKGAVKIFEAYLQGKLPMKADWNGKYYHSKVAV"
     gene            3508..4299
                     /locus_tag="PXE44_000606"
     CDS             3508..4299
                     /locus_tag="PXE44_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5588147.1"
                     /translation="MSMLLMVKAMNCTVGNSARKLVLLKLADNANDDGVCFPSYQFIA
                     DKCEMSKRSAISHIDELIKMGYVSKKSRKNKDGSSSNLYQLHLDAPSEKSALGGENFA
                     PGGSENSAPITSHSINQSINHNTSEQNSDGVDKKSKYRFSDNDMAVAKWIFKRVKQLA
                     PQTKESYLPNWANEVRLMVERDKHTHQEICSLFKFATEHHFWGRNILSVVSLRAKWDR
                     LNLERQSSLRPNNIHSATGNGDIQDTTGFSKGLVVDVGGGTKWEF"
     gene            4287..4922
                     /locus_tag="PXE44_000607"
     CDS             4287..4922
                     /locus_tag="PXE44_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL5588148.1"
                     /translation="MGILATTTQNQVLPVEIVEQAEVETTKIFNRLKATHLGWRANIK
                     SQAEYDNARREWVRLLIENRLTPKEVERGLKIADTDKNPYLPSFGQFLEWCKSIDYAE
                     LGLPDMDQFLKRLNHFSAFGFTEIDNFKFRSNAEYWLLTDLYERNRKHSWKDETLRNQ
                     AEKALVEMAKRIMSGEQIPPPKPVIEEKKKVPIDPRIQRILDEKKQAGAMQ"
     gene            4919..5491
                     /locus_tag="PXE44_000608"
     CDS             4919..5491
                     /locus_tag="PXE44_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5588149.1"
                     /translation="MTDFDKDTYQTPNYVRNWLNHRYAWFHIDGCSNGKNALYSHWIG
                     KAAEGLDEDTLSGQIAEDFLAENLLDVILECVADWGELLRIFVNPPYSNPLPFVQRAA
                     ELKKAGHIVVMLLPADKSTRWYQVIQENASEVIDIIGGRINFINPLTDEEVKGNSKGS
                     MVAVFDPFVEGIVTRQMPLDKIKELGGYEK"
     gene            5561..6589
                     /locus_tag="PXE44_000609"
     CDS             5561..6589
                     /locus_tag="PXE44_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDL5588150.1"
                     /translation="MAEGLLLTPYFQTEVGIVFYKVPKDVSLAAFGERTLLQPAPTDL
                     ATVKSGKVANFSANSTACKELAEFAKSAKVRSTLNQRDKYSNDLPYTRFVKSIQTCQC
                     RDGKYCHHEKVFVENEKGAVQLCWYHDKMRMDGEIPNEKLEALADENWEKFIAQQIRL
                     TLRKTQSSAIEFADLVLFASIKGLLDELDSNALQQFLGYPPLNNSGVSKESNIGFDDV
                     PNARANLKKWAETLKLKVEPEPLAAFMAKPKLQRFEWRKWLQFVKAQPCMCCGQQADD
                     PHHIIGYGGAMGSKQHDLFTLPLCRIHHDELHRNVGKFEQDYGSQLELLIKFLDRAIG
                     LGALEIDG"
     gene            6582..6941
                     /locus_tag="PXE44_000610"
     CDS             6582..6941
                     /locus_tag="PXE44_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL5588151.1"
                     /translation="MVELVLPYPPSVNDYWRWVSKTRRVVCKRGKDYQWATFLAAQNK
                     PKFGGQVEIKCDVYFPDNRDRDLDNLGKCILDSLVYSKVIVDDSRHYVKKLTFEDKGN
                     QKGGAVIVRIKERLDVN"
     gene            6931..7290
                     /locus_tag="PXE44_000611"
     CDS             6931..7290
                     /locus_tag="PXE44_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588152.1"
                     /translation="MLIDIKGILRFWGYSANGRLGTEFPCVAAGMKPAVSASNHRILR
                     LTDESIFEIDRCIKQLKEQEPQQYEVLIGRYAARVSDTQIEKVLGISHSTFKRELAQA
                     EMYVLGVVVGLKLALVV"
     gene            7370..8176
                     /locus_tag="PXE44_000612"
     CDS             7370..8176
                     /locus_tag="PXE44_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL5588153.1"
                     /translation="MKKLVLVTALSLVLSACGSGGGGGSSASNPNKEATPQSPSKSET
                     KSDTSSQNQVKGALSKVSADLQEQVKALKQINVDGRIIELAQEPIGFIEKDLDNGKKG
                     KAYNLAYSSIGYVLPKDVKTDEYGRVIDERASANDVGIFGLATKFQDLPKSGTAHYSG
                     VSFGANSEGKLTLTADFGDKKVNGSITERKLLSNNQALSSINLLSAPISQTKFAGEEI
                     HFAGIAQANVEGNIVQAPYGGKFMGPKAEEVVGFVADDHNDPYEVFAGQK"
     gene            8340..8687
                     /locus_tag="PXE44_000613"
     CDS             8340..8687
                     /locus_tag="PXE44_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDL5588154.1"
                     /translation="MPEKNPDIYTQAWNYFAMFVSNHSSFICAVIIAFFTSITKSFLY
                     GKTDTPKRVFAEAILCAVIAGSLRPILIYFNADVSLITPIGAGIGLLGTSAIRQMILK
                     FLNKKAGVDDNVN"
     gene            8677..9273
                     /locus_tag="PXE44_000614"
     CDS             8677..9273
                     /locus_tag="PXE44_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HDL5588155.1"
                     /translation="MLISERKFKQVFPSAIDGIYAEIAKHIEKAGCKTKEQQAMFLAQ
                     CGVECAGYKTFEENLNYSATALLATFPKYFTQHNVVLYARDKRAIANRVYANRMGNGN
                     EQSGDGWKYRGRGLIQITGKANYIEFAKWEGLPSLVTSPDQITDNLALTVQVAVWYWQ
                     SRKLASYGSDILKVTKLINGGTHGLSQRTNYYQRLMVA"
     gene            9274..9624
                     /locus_tag="PXE44_000615"
     CDS             9274..9624
                     /locus_tag="PXE44_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL5588156.1"
                     /translation="MSKYIYLVATILILGLCGWIWQQSKQINTLRAENQTQAQTIEQQ
                     QKANQRLADKLEQERQAVEKIRKSANEMRNKVEIVQNEIKSILAQDSCAKADLPNGVA
                     DSIKRLHRQNNHKH"
     gene            9629..9802
                     /locus_tag="PXE44_000616"
     CDS             9629..9802
                     /locus_tag="PXE44_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588157.1"
                     /translation="MYPPAAYLVPCERTEFSGNTYGDTVEYLIKVMGERDLCANQIDR
                     IREWQAQTKQGFK"
     gene            9938..10336
                     /locus_tag="PXE44_000617"
     CDS             9938..10336
                     /locus_tag="PXE44_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588158.1"
                     /translation="MIVKLKLGAVVVLSQVKQATIAYAGSEAYQTAIEQAKKGLLDED
                     KGKPFETVLHFPAVYADEDGQEEICPEQIATTVRSDNVSGEPVAVVLIPDDNVVGELQ
                     SIFIYKTDYAEIFTDNGVKLSAVRTSPAPR"
     gene            10484..10960
                     /locus_tag="PXE44_000618"
     CDS             10484..10960
                     /locus_tag="PXE44_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDL5588159.1"
                     /translation="MDNLFDLKLNINQIAELTGLHRQTVSQRVAGLNPALGSNSKLKL
                     YALRDLILTGLAEKMSADVDSLSPADRRAWFQSENERLKFEKEIGELIPASEVALEMS
                     ALAKTVVQALETLPDILERDCGLQPKDLIRVQQVTDDVRDQMALHIQEVTTDTENE"
     gene            10960..13071
                     /locus_tag="PXE44_000619"
     CDS             10960..13071
                     /locus_tag="PXE44_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDL5588160.1"
                     /translation="MFASAKEIRRDIANAIKAPRRMRVSEAVAEYMRVPLGGGNSVKW
                     DKHTAAYMLEPMDCLNSREYDAVIFVGPARTGKTIGLIDGWITYSIICDPSDFLLVQL
                     TQEKASEHSRKRLDRTFRCSPEIAKRLSPRKNDNNVHDKYFRAGNLLKIGWPSINVLS
                     SSDYKYVALTDYDRWPEDVDGEGDGFSLASKRTTTFMSAGMTLVESSPGKDIVDLKHI
                     PKSTHEAPPTTGILSLYNRGDRRRFYWQCPDCSEYFEPSMANMVGYREDSDFVKASEN
                     ARLQCPHCQTLIAPELKRELNIKGVWLKEGQKIDASGQISGESRKSRIASFWLEGPAA
                     AYQTWAQLTYKLLNAEHEYEMTGSEETLKAVTNTDWGLPYLPRSALEQRRSDELMERR
                     EEVEEKTVPAQCRFIVAAVDVQGGKNRRFVVQMVGYGENGERWLIDRYNISHTLPDSD
                     GVIEKIDPRIPDDWYILISDVLEKRYPLAHNPNHFMPILAMAVDSGGEDGVTDNAYQF
                     WRKCRRDGYAKRVYLVKGDSTKRQKLITKTYPDNTARSDRHSSARGDVPLYLLQTDYL
                     KDRINNALFRDTVGANYIHFPDWLGEWFFNELTYEERGADGKWRKPGKGNNEAFDLFC
                     YAHAIAILRGYERIKWGDENDVPSWAKLPDINPNIIRETPRTAEQAVEIEEKSEKPQP
                     KPTKAKSSWLSGGGRKTGGWL"
     gene            13068..13292
                     /locus_tag="PXE44_000620"
     CDS             13068..13292
                     /locus_tag="PXE44_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588161.1"
                     /translation="MSLYTIDELKQKIRTLDEKIETAQSQVSFNGRSVSYQVAELTKQ
                     RDRYQTMLDEQLAQSGQRTKQHRIKYARFI"
     gene            13292..14791
                     /locus_tag="PXE44_000621"
     CDS             13292..14791
                     /locus_tag="PXE44_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL5588162.1"
                     /translation="MNFLEKTIATLSPKWAANRSRNRYVLNAYEAAQPSRTHKASRES
                     KGANSTVRQSAVSLREQARALDQNHDIVIGILDKMEERVIGSKGIHIEPQPLTLAGEV
                     HEELAGQIRKLWAEWSVKPDVTGLYTRPLLERMLLRTWLRDGEVFVQLVKGKVAGLEH
                     GSQVAFSLEALEPDFVPMQSDEAKNGLVQGVFLNAWRKPTAYQVYLDNPQESNGMYGK
                     VKTVPADNMLHLAFRKRLHQIRGVSMLHGVIVRLADLKEYEESERVAARIAAAMTMYI
                     KKGDAALYDDDSNSDGERLFDIAPGAVIDDLKPGEDIGLINSNRPNVNLETFRNGQLR
                     ATAAGTRSSYSSIARDYNGTYSAQRQELVESFEGYAVLQDAFVAAISRPIYREWLKMA
                     IASQAIKLPPDIDQNSLFNAVYSGPVMPWIDPIKEANAWKERIKGGLATEGQAIRASG
                     NNPAEVKRQRIVEIKENQAEGLKFDTDLTNYGGQDKSFSNQKDEINEDE"
     gene            14803..16764
                     /locus_tag="PXE44_000622"
     CDS             14803..16764
                     /locus_tag="PXE44_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDL5588163.1"
                     /translation="MKTWFNIQAKANDTAEISIFDEIGFWGVTAQQFAKDLKALGNNL
                     KQINLHIHSPGGDVFDGIAIYNLLKNHPANKTVYIDGLAASMASVIAMAGDEIIMPEN
                     AMMMIHKPWGIQGGDADDMRKYADLLDKVESTLIMAYVAKTGKYETDLAEMLKEETWL
                     TGKECVEQGFADKLAEPLVAMACIQSKKLEDYTKMPDKIKNMLFAPQGNAGANPKHEQ
                     PQTPPQAGENPQNVAKPDAMVALAQRNATIKATFAAFGTQFDGLLAECLADVSMTAEQ
                     AKDKLLAKLGENTTPSVPQNHIHAGNGNIVGDSVKQSLMARAGQDTDKTNARDNAYNA
                     MTLRELARASLVDRGVGIAGYTPMQMVGLAFTHSSSDFGQILIDVAHKSLLKGWETAT
                     ENYEQFTTRGTLTDFRPAKRVGLGEFGYLPEVGEGEEYTYGTIGDEGASVALATYGQL
                     FSITRQAIINDDMHLLTKIPEKMGAAAKATITKLVFALITGNATAQDGKKLFDATHKN
                     SLTGAALNVENIDKAIQLMNGFVNSRGEPLSIEPEFMLLPTSLHTKAKQILGSSSVEG
                     ADINSGIINPIRDIVSPIKSPRLQIADPKSWYLLNKEAIEVSYLDGVDSPYIEQQHGF
                     TVDGVSTKVRIDAGVNVIDYRGVVKVTNA"
     gene            16838..17161
                     /locus_tag="PXE44_000623"
     CDS             16838..17161
                     /locus_tag="PXE44_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDL5588164.1"
                     /translation="MAKNYIQDGNTVRLTATKAITSGDVIVAEDLIAIAVSDAAKNDA
                     VVGLTTGVFSVKAKQADDIKQGAVLYWSETEGATLTAGSNKRLGIAWKDSGTSSNQVD
                     VKINV"
     gene            17154..17456
                     /locus_tag="PXE44_000624"
     CDS             17154..17456
                     /locus_tag="PXE44_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5588165.1"
                     /translation="MSSPFEQAIAAADQTIQQTMMSEWLIGGKPYPATFDEAPAIFNG
                     LHSTDDRAIHGTERTLTLFRASGYKPRLDDRVSGNGKKYLVKSYHFVDQLIVLQLE"
     gene            17460..17984
                     /locus_tag="PXE44_000625"
     CDS             17460..17984
                     /locus_tag="PXE44_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5588166.1"
                     /translation="MGSQVTGLKELQASLKKIARQTVPKAAAQAIRTVGRQAMNKAVK
                     SVAAEIGVNQKTIKGRAKMTAKPTPSRLQATIKVNRSHMPMIRVLERKSNRLSPTKGS
                     IRVGKHTVQRGFRQRLANGRTHIMYRQGRKRYGIDVAKVPLSQPLTQAFDRELKNYPE
                     QVQAELAKQIAGKL"
     gene            17981..18376
                     /locus_tag="PXE44_000626"
     CDS             17981..18376
                     /locus_tag="PXE44_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5588167.1"
                     /translation="MKIHTKIRKEVLELLQTKLPEIDNFYNGLPTFIDIEEEQLAVSV
                     YLDDISLQEITVCNNQWDAKLNITIHLKSTESAEDELDEWAEKIREIIDDYPEFEHLE
                     SVALSQYQYEQDQNQRTWHMATLIFDVEY"
     gene            18404..19045
                     /locus_tag="PXE44_000627"
     CDS             18404..19045
                     /locus_tag="PXE44_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588168.1"
                     /translation="MAKTTKVQGTKFRISVGREAQKAITAINLNTATLTIASSGYKKG
                     DAIELTGCGQLDGIYPVLSVTTDQVKLCEEVNWTGKDLPANYTKAKAALVQYSEQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPTTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            19129..19530
                     /locus_tag="PXE44_000628"
     CDS             19129..19530
                     /locus_tag="PXE44_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588169.1"
                     /translation="MTLREKLLANKPKLQPIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGVLLFNPLDINDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            19575..19805
                     /locus_tag="PXE44_000629"
     CDS             19575..19805
                     /locus_tag="PXE44_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL5588170.1"
                     /translation="MPERHFAEYRLFYQEQPFGLWRDDYRTAQLAHLTAMFNRDPKGK
                     APKLAEFMPFFNRTDEVEEIEDDGVADYLAKR"
     gene            19868..20095
                     /locus_tag="PXE44_000630"
     CDS             19868..20095
                     /locus_tag="PXE44_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588171.1"
                     /translation="MFEALDRFMINTIKIAIIVFLVACIFISMPNWWIASILVLFPTA
                     YLLDKYWWRNTEMYRRKMDKLVEEMKNNRQI"
     gene            20149..23715
                     /locus_tag="PXE44_000631"
     CDS             20149..23715
                     /locus_tag="PXE44_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL5588172.1"
                     /translation="MSSSNRLTITLDVENSKLLAGFAQSDTVANKFARSFIVNMDQAQ
                     QKARQFADRSTKYLKNIEQAANNLNSSVSKERFWQIANRLQFPTSKVISYANSYTELG
                     NKLKLVTESESQHARAMAAVYDISLKTAQSTQATSSVYQTFAQNAKQLGISQNDVAKL
                     TETVAKSVAISGASSATASNALVQFSQSLLMGKLKAQEFNSLMTQTPSVIQAIAKGLG
                     ITTAELKAMVDNGEMSAEKMIEGLKKAESYVNGQYAKTTTTISGAMQNLSTAAEKWVG
                     EMDSTVGVSQKAVAVLNALNANFDTVAKTVLVAGGAFALFKGYMNVRPFIESRAAIYE
                     NQKAIIAENQARQQAMAIRQQELVGLSQQTALKLEQTLQTYNQNKAELEAIAVKQQKI
                     TKEREEIAVAIQAAATRSERLALSAELQALDQKEITLGQQKIAVERQQIAAKQALKVA
                     YAENAVAQSAMSAGMITATTTTRAMNGVLAAARAELNLLKAAALSNPLMTLAMVATTA
                     GTALWGFFESQKAAREEALRYADSLDSVRVNIEKMTKAQTDAEMVKLSRSIKEQETAL
                     ANLKQRQAELTREIEQGATVYEDAFAGTVRVTMSAEELAKKQEELTLVTADAEKAQKT
                     LNSSLEAQEALKAHIPIANLREEFVKLYPHIDESQIKVDGLNIAIGNFTVKSPEMVIA
                     TNNIANALGGVAGEAMRAAILVANLSSMKLDANGAAIIDPKHLEKIEQIERNNAIGSA
                     KGKDKIALQVKDALIKSGMKEGDAGYERLKAAYEQQFTLQNAPKGKGGKTKAEKEAEK
                     AAKKQQSYQNQVAEMTNRLAGLKANASDIAIFGQVSDYQEVRKLTEDIAINAEKYKGY
                     GEQGVARLKELASQLESAQQQVAISQFAYNGGEKLKAMEFELTLLGKTRQEQELMQYN
                     HELDLEAARLKIGMADENIAKLDQEIVKLKERRAEIQAQAEQARGSFKQGMLDGLNNI
                     EADVSNVAANVANITQNTFDGMADSLTNFVMTGKADFRSFANSILSDLSKMIIKMMLF
                     NAIKQGATMLFGYQGPTTTVNGNAVYGLGTWATGGYTGDGGKYTPAGIVHKGEYVITK
                     EATARLGLDYLNYLNYGKRGFSSGGGVAVPRVPSSSYQPKSAQSNISVQVINNGEPVD
                     AKVSQKQQGDQMQITVELMREIARQEANGMIQNNFRAGGVFA"
     gene            23715..24044
                     /locus_tag="PXE44_000632"
     CDS             23715..24044
                     /locus_tag="PXE44_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5588173.1"
                     /translation="METLKWCVRTDLAVENTPEIMEVKFGDGYTQRSPKGLNNLLRAY
                     TGTIKVKKGEHLAVEAFFAKHRGVSPFYFKDPYTQQNKKVVCSSWPAKMVGLNHWEFS
                     VTFKEVP"
     gene            24044..>24165
                     /locus_tag="PXE44_000633"
     CDS             24044..>24165
                     /locus_tag="PXE44_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5588174.1"
                     /translation="MPVSISNQMKLDLAKLEQNAMLDLYEVDLRTLKDKSGNAGS"
BASE COUNT         7528 a         4880 c         5465 g         6292 t
ORIGIN      
        1 tctataagat atttattgag gttaattttt gtagccgacc ttttttctta ttgatatata
       61 ggttttctcc tttacatcga atttgctgtt cggttttgtg tgcttcagct gccaatttgt
      121 actgctcagg aggaaggtca accctaatgt caaccgtttt atcttctatt ttacaaggta
      181 cctgaatata acctccatca gctaaattgc tggagtgtaa ccctacaact ttgccaatga
      241 tttcatagtg tgggagggta tattctcctt ggtgatacct agatgcaacc tgaattactt
      301 taatttcttt ttgagaaaat ttaatattaa atgtttcaga aggcattgat tcatcactaa
      361 tttcacccac ttggagttta atttcgattt ttctttgttg gtctgaacca cttaatccaa
      421 taatagcttc acaaagatta tgacttactc cttcaggtat caattcagca aaaatagtcg
      481 gattgtgatc ataatgagta attttttctt tcagcttgtt caggctgtta accaaattat
      541 gcgatacact ccgagaaaat gaaattgcct gaataatttc attctgttcc ttaatttcaa
      601 tattctcaac aggataagat aaattgacaa tatagcttcc aatttgagtt tgccctaaac
      661 gaacttgaga cataaactct gctacatctt tgccacctga gttatgtcca aaagctgcct
      721 ttttcttttt agctgaaagt gccaaggcat taattaaatg tttggttttt tcaaataatt
      781 ttacaccttc gtttagcgga attgtcccat ctttaacgct ttcgccaatc actctaacag
      841 atagcttgtc tgtatattga tttgaaatat taagctttaa cgtttctacg tttttttctt
      901 caattttggc gagcttttca agtgctttct gcaacacttc ctctctatct tcagcaattg
      961 gctttggtaa tgtaacctct aaaattcgat gtccaagatc tttctgccaa ataaccgcat
     1021 actctaattc aaacgcaatt tgccagcttt tggcagttaa atagcgagca aactgttcga
     1081 gaaacagtaa cgtgtccgtt ttattgtttt tcataaagtt tctcctactc tagcgtattc
     1141 aatcatttta atgagtacat cagtggtaaa tcgttgattt tctggaaatg tgagggtaac
     1201 actacttcta tttgttgttg ccggaaagcc ttttaatgat acccaataac aatgtctttt
     1261 taaagtcata aattttttct tatgtgagag ccattgagtc gtttttttag gcaagtcaca
     1321 tacaattaag tagcgagggt tcgcacaatt tttccttaaa tcatcatagt ttttcttttt
     1381 gagtttatag cttaccagac catttttaat atgaagatct ccttctgatt taagttgtag
     1441 ctttatttcc ggatcacttc ttttcccttc ttcagtcgga aattctgcag aaattgcaag
     1501 atcaacacta tgattgtcta ctactggtac cgcatgatta aagcctgcat tagcagtaat
     1561 agctaacaca taagcaatat taaattgttc catttgttgt gtaggtgaaa gcatatttct
     1621 atccaataaa attcaactgt tggctttggt gaacttttac tctagcatgg ataaatagct
     1681 gatcttgatt ttcttcagtg atactccact tttcatagag tttgttatct gaaatcacta
     1741 atatttgatc gcctgtattt tgtaatcttt ttacatacaa atgtcctttg tatgtaaata
     1801 cataaacacc atcgccggta aactcattta cgctaatatc aacaaaaagc atatctcccg
     1861 aggaaaatgt cgggtacata gaatcaccac ttaaattgat aattctgatg tatcgaggtg
     1921 ccatattccg aaagagggaa taaaattgct caggcacata acgcatttga tttactactt
     1981 caactaattc actgcttgag ccgaagcctg cactagcact gacatctaat acatcaatta
     2041 tgataaagtc atttaaatct tgctcctgca atttttgttc ttcattgcct atccttgaat
     2101 tgataaggtt tgattgctct gaatgtattt gatctaacca gcctctaggt ttattcatcc
     2161 catcttctaa tttggcggca atcttgtcac caatgccttt tggcttaccg ttctgaatag
     2221 catttttatt tttgatttgt agtagatagc ttactgcgat acctgttcga ttagctaaat
     2281 cagatgtgct tttagcctca tctatcaagc gtaataaatt atcgtatcta atttctttta
     2341 ggggtctcat atcccacctc catactattt gctttatttt attatcattt cgataacatt
     2401 taaatatgat ctattttgtt gattttgatt tatctttaag ataatattca cttatctttt
     2461 aggtaaggat tatgaaatgc aattacaaga atacatagaa aaatctaagt ggggggaaat
     2521 ctctcgatta gctaaagcca tcgatgtagc aattccaata gtttcatttt gggtaaacgg
     2581 caaacgccaa gtaccggcaa agcgttgccc ggaaattgaa aaatttacag aagggaaagt
     2641 aacttgtgaa gagctacgcc ctgatgttga ttgggcagtt ttacgaaatt caggcaaata
     2701 aaaaaccacc gctggaacgg tggctaatta caacctaagg aggtttattt tgaaatccca
     2761 atcattatta ccgataaccg cacaaaatgc aagcggtgcg gaagtcaaaa tcagcagtat
     2821 tgaaattgca gagttatgcg aaaaacaaca taagaacgtt attgtagata ttcgcaagat
     2881 gttagctgag ctgaaattga actcggctga tttttcagcc cagtatcaaa ccaaagacgg
     2941 cagaatgcaa ccctgctact ttcttccaaa acgagaaaca atgattttag tttccggcta
     3001 tcgcattgat ctgagagcca aaatcattga ccgcttagat gagttagaaa agcaacatca
     3061 gccctctatt ccgaaaaatt accccgaggc tctgcgaatg gcagcggagt tggcggaaca
     3121 aaaccaagtt ttattgttgg aaaaacagca gactaccgca gaaaaccacg ccttgaaatc
     3181 ctactttgaa ccgggtttga cccctgctca gtttgtaaaa gggctgaatg gcgtaaatag
     3241 taacaagata aatgattatt tacgtagtcg aggctggtta tacaaagaca agcattgctg
     3301 gcgggtgttt agccacgttc gggataaata tctgacggag tcatttaaga gaattgaagt
     3361 tgatcctgtt gaacgtattg agatggccac ctacaagcca attttgcttg agaaaggtgc
     3421 tgttaagatt tttgaggctt atttacaagg taagttacca atgaaagccg attggaacgg
     3481 caagtattat cacagtaagg tggcggtatg agtatgttat taatggttaa ggcgatgaat
     3541 tgtacggtgg gaaattctgc ccgtaaatta gtgctgttaa aactcgctga taacgcaaat
     3601 gatgatggtg tttgctttcc tagttatcaa tttattgcag ataagtgtga aatgtctaaa
     3661 cgcagtgcta ttagccatat tgatgaatta atcaaaatgg gatatgtgag caaaaaatca
     3721 cgcaaaaaca aagatgggtc tagttcaaat ttatatcaat tacaccttga tgcccctagt
     3781 gaaaaatctg cactaggggg tgaaaatttt gcaccagggg gtagtgaaaa ttctgcaccc
     3841 ataaccagtc actctattaa ccagtcaatt aaccataata cgtccgaaca aaattcggac
     3901 ggggtggata aaaaatcaaa atatcgtttt tctgataacg atatggcagt ggcaaagtgg
     3961 atttttaaac gagtgaaaca actagctcct cagaccaagg agtcttactt gccgaattgg
     4021 gccaatgaag tccgcttgat ggtagaacgt gataaacata ctcaccaaga aatttgctcg
     4081 ttgtttaaat ttgcgaccga acaccatttt tggggcagga atatcctaag tgttgtatct
     4141 cttcgagcta agtgggatcg gctaaatcta gaaaggcaat catcattacg accaaacaat
     4201 attcactcag caacaggcaa tggtgatatt caagacacaa cggggttttc aaaaggtctt
     4261 gttgttgatg taggaggagg gacaaaatgg gaattttagc tacaacgact caaaatcaag
     4321 tattacctgt tgagattgtg gaacaagcag aggtggaaac aaccaagatt ttcaaccgct
     4381 tgaaagcaac tcacttaggc tggcgagcca atatcaagag tcaagccgaa tatgataatg
     4441 ctcgccgtga atgggttcgt ttgttgattg aaaatcgctt aacgccaaaa gaagttgaac
     4501 gaggtttgaa aattgcggat accgataaaa atccctattt accgagcttt ggacaattcc
     4561 tcgaatggtg taaatccatt gactacgcag aattaggttt gcctgatatg gatcaattcc
     4621 tcaagcgttt aaatcatttt tccgcttttg ggtttactga aatcgataat tttaagtttc
     4681 gttcaaacgc tgaatattgg ctcttaactg acctttatga acgaaatcga aaacatagtt
     4741 ggaaagatga aacattacgt aaccaagctg aaaaagcgtt agtagagatg gcaaaacgga
     4801 ttatgagcgg tgaacaaatt ccacctccta agccagtaat cgaagagaaa aagaaagtgc
     4861 caatagatcc acgcattcag cgaattttgg acgagaagaa acaagcagga gcaatgcaat
     4921 gacagatttt gataaagaca cttaccaaac acctaattat gtgcgtaatt ggctaaatca
     4981 tcgctatgcg tggtttcaca ttgacggttg ttctaacggc aaaaacgcat tgtatagcca
     5041 ctggattggc aaagcggcag agggcttaga cgaagacaca ctaagtggtc agattgccga
     5101 ggatttcttg gcagagaact tgctcgatgt gatcttggaa tgtgtggcag attggggcga
     5161 attactgcgt attttcgtga atccgccata cagcaatcca ctgccgtttg tacaacgggc
     5221 ggcagagctg aaaaaagccg gccatattgt ggtaatgctt ttaccggcag ataaatctac
     5281 ccgttggtat caggttatcc aagaaaacgc tagtgaggtg atcgacatta tcggcggacg
     5341 tattaatttc atcaatccgc tcacagacga ggaagtaaaa ggcaacagca aaggctcgat
     5401 ggtggcggtg ttcgatccgt ttgtggaggg aatagttacc cgtcaaatgc cgttggataa
     5461 gattaaggag ctgggcggct atgaaaaata acgaaggctg ggacggttta gataaggcgt
     5521 atcattgcct tagccgcaac aaaaagaaat gggggcaata atggcggaag gtttgctact
     5581 taccccttac tttcaaaccg aagtgggtat tgtgttttac aaagtgccta aagatgtttc
     5641 tcttgctgct ttcggtgagc ggactttgtt gcaacctgca ccgaccgatt tagcaacggt
     5701 taaatcaggc aaggttgcaa atttttcggc aaattcgacc gcttgtaaag agttggcaga
     5761 gtttgctaaa tcggcaaaag ttcgctcaac gctaaatcag cgtgacaaat acagtaatga
     5821 tttgccttat acccggtttg taaagtcaat tcagacgtgc caatgccgag atggtaaata
     5881 ctgccaccac gaaaaagtat tcgtggagaa tgaaaaaggt gcggtacagc tttgttggta
     5941 tcacgacaaa atgcgaatgg acggtgaaat cccgaatgaa aaattagaag cactagcgga
     6001 tgaaaactgg gaaaaattta tcgcccaaca aatccgctta accttacgca aaacgcaatc
     6061 ttctgccatt gaatttgcgg acttagtgtt gtttgcgagt atcaaaggtt tgcttgatga
     6121 attagacagc aacgcattac agcagtttct cggctatcca ccactgaata atagcggtgt
     6181 gagcaaagaa agtaatatcg gatttgatga tgtgccgaat gctcgagcca acctcaaaaa
     6241 gtgggctgaa acgttaaaac tgaaagtcga gccggagccg ttggcggcat ttatggcaaa
     6301 accgaaatta cagcggttcg agtggcggaa gtggttgcag tttgtaaaag cacagccttg
     6361 tatgtgttgc ggacaacaag cagacgaccc acatcatatt atcggctatg gcggagcgat
     6421 ggggagcaaa cagcacgatt tattcactct gcccctatgc cgtattcatc acgatgaatt
     6481 gcatcgcaat gtgggtaaat ttgagcaaga ttacggctcg caattagagt tgttgattaa
     6541 gtttttagac cgagcgatag gtttgggggc gttagaaatt gatggttgaa ttggtgctgc
     6601 cctatccgcc aagtgtaaac gactattggc ggtgggtaag caaaacaaga cgagtggtgt
     6661 gtaaacgagg caaagactat caatgggcaa catttctcgc agcacagaat aaacctaaat
     6721 ttggcggaca ggttgaaatt aagtgcgatg tttacttccc tgataaccga gatcgggatt
     6781 tggataactt aggcaaatgt attttagatt cgttggtata tagcaaagtg attgttgatg
     6841 actcccggca ctatgtcaaa aagctgacat ttgaggacaa gggcaatcaa aaaggcggtg
     6901 cggtaattgt aaggattaag gagagactag atgttaattg atattaaagg tattttacgg
     6961 ttttggggct attccgccaa cggcagactt ggtacggaat ttccctgtgt ggcggcaggg
     7021 atgaaacctg ctgtatcggc aagcaaccac cgcatattac gtttaactga cgaatccatt
     7081 tttgaaattg accgctgtat caagcaactt aaagagcaag agcctcagca atatgaggtg
     7141 ctgattggtc ggtatgcagc aagggtatct gatacacaaa tcgaaaaagt attaggtatc
     7201 agtcactcca cctttaaacg agaattggca caggcggaaa tgtatgtgct gggtgtggta
     7261 gttgggttaa aattggcgtt ggtggtttaa ataatgtgat ctgtatcaca acattaattc
     7321 aataaatgag ataaagtata gactcttttt acctatggag attttaccaa tgaaaaagtt
     7381 agttttagtt acagcattaa gtttagtttt atctgcttgc ggtagtggtg gaggtggtgg
     7441 ctcatcagca tcaaatccta ataaggaagc tactccacag tccccatcta aaagtgaaac
     7501 taagtcagat acttcatctc aaaatcaagt aaaaggtgct ttatctaaag tttcggcaga
     7561 tttacaagag caagtaaaag cacttaagca aattaatgtt gatggtagaa tcattgagtt
     7621 agcccaagag cctatcggtt ttatagaaaa agatttagat aatggcaaaa aaggaaaagc
     7681 ctataactta gcttattcta gtattggtta tgtgttacct aaagatgtaa aaacggatga
     7741 atatggacga gtaattgatg agcgagcttc tgctaatgat gtcggtatat ttggactagc
     7801 aaccaaattc caagatttac caaaatccgg aactgcacac tactctggcg tatcatttgg
     7861 tgcaaacagt gaaggtaaat taacacttac tgctgatttt ggtgacaaga aagtaaatgg
     7921 ctcaattact gaaagaaagc tattatctaa taaccaagca ttatcttcta ttaacttatt
     7981 atctgcccct atcagtcaaa ccaagtttgc tggtgaagaa atacattttg ccggcattgc
     8041 tcaagccaac gtagaaggta acattgttca agctccttat ggcggtaaat ttatggggcc
     8101 aaaagcggaa gaagttgttg gtttcgttgc cgatgatcat aatgacccat atgaagtatt
     8161 tgctggtcaa aaataatttt taatgtatga ttgacaaggt cgccctaaaa gtataggatt
     8221 tatgcaatcc ttgcgattag tgcaagtgca ttacgcaata ctatttttat agccctgatt
     8281 ggttcagcca gtcggggctt ttttgttgcc ccgaaaaagt ggggtggagt atgaatacaa
     8341 tgcctgaaaa gaaccctgat atatatacgc aggcttggaa ttattttgca atgtttgtca
     8401 gcaatcatag cagttttatt tgtgctgtta tcattgcttt ttttacgtct attaccaagt
     8461 catttttata cggcaaaacc gacacaccaa agcgggtctt tgcagaagcg attctgtgtg
     8521 cggtgattgc cggttcgttg cgtccgattt taatctattt taatgctgat gtttccttaa
     8581 ttaccccgat tggtgccggt atcggactac tagggacaag tgctattcgt caaatgattt
     8641 tgaaattcct caataagaaa gcaggagttg atgataatgt taattagcga aagaaaattt
     8701 aaacaagtat tccccagtgc gattgacgga atttatgccg aaattgccaa acacattgaa
     8761 aaagccggtt gcaagacgaa agagcagcaa gcaatgtttt tggctcaatg tggcgtagag
     8821 tgtgctggtt ataaaacctt tgaagagaat ttgaattatt ccgctacggc attattagcc
     8881 acatttccga aatattttac ccaacacaat gttgtgcttt atgcccgaga taagcgtgcg
     8941 attgctaatc gggtgtatgc aaatcgaatg ggtaacggca atgaacaaag tggcgatggt
     9001 tggaaatatc gtgggcgtgg gctgatccag attactggta aagcgaatta tattgagttt
     9061 gcgaagtggg aggggttgcc aagcttggtg accagtcctg accaaatcac agataacctt
     9121 gctcttacgg tgcaggtggc ggtatggtat tggcaaagtc gcaaattagc aagttacggt
     9181 agtgacattc taaaagtaac caagctgatt aacggcggca ctcacgggct atcacaacgc
     9241 acgaactatt atcaacgatt aatggtggca taaatgagta aatatattta tctcgtagcc
     9301 accattttga ttttgggctt gtgtggttgg atttggcagc agtccaagca gattaatacc
     9361 ttaagagccg aaaaccaaac gcaagcccaa accattgagc agcagcaaaa agccaatcaa
     9421 cggcttgctg ataagctcga acaagagcga caagcggtcg aaaaaatccg aaaaagtgca
     9481 aatgaaatgc gaaacaaagt ggagattgtt caaaatgaga ttaagtcaat ccttgcccaa
     9541 gacagttgtg ctaaagctga tttgcctaat ggcgttgctg atagcattaa gcggttgcac
     9601 aggcagaaca atcacaagca ctgaatattt gtatccgccg gcggcttacc ttgtgccgtg
     9661 cgagcggact gaatttagcg gcaatacata cggcgatacg gttgagtatc tgataaaagt
     9721 gatgggagag cgtgatttgt gtgctaacca aatcgaccgt atcagagaat ggcaagcaca
     9781 aaccaaacaa ggttttaaat agcttggcgg attaagccgc tatgacatat gggagcgtaa
     9841 cagctccctt ttttattgta ccgcattact tagttatgct tttggttata tcaagaccat
     9901 aactaagcaa tgtgttctat taacataagg attatttatg attgtgaaat tgaaattagg
     9961 tgcggtagtt gtgctatcac aagttaaaca agccaccatc gcatacgctg gatcagaggc
    10021 ataccaaaca gctattgagc aagccaagaa aggtttactt gatgaagaca aaggtaaacc
    10081 atttgaaact gttcttcact tccctgctgt gtatgccgat gaagatggac aagaagaaat
    10141 ttgccctgaa cagattgcaa ctacagtacg ttcagataat gtaagtggtg agcctgttgc
    10201 agtggtatta atcccagatg ataacgtagt tggcgagtta cagtctattt tcatttataa
    10261 gacagattat gcagaaattt ttactgacaa tggtgtgaaa ttaagtgcag taagaacaag
    10321 tccagctcct cgttaatttt gtaaaaggta ctcccgaggg gatacccctt tccacggggt
    10381 tgcggcagcg tggttttcgg caattttttg agtttctagg catcatcatc ttcctatgat
    10441 gttgctgttt tttaggcggt tttgattttt aggatttgag caaatggaca acttatttga
    10501 tctaaaactt aacatcaatc agatcgccga actcacagga ttgcaccgcc agacggtatc
    10561 tcagcgagtc gctgggttaa atccagcact cggtagtaat agcaaattaa aactctatgc
    10621 tctgcgtgat ttaatcctca ccgggttggc ggaaaagatg tcggcagatg tcgatagcct
    10681 atcacctgct gatcgtcgtg cgtggttcca gtcggaaaac gaaagactaa aatttgaaaa
    10741 agaaattggc gagcttatcc cagcaagcga agtggctctt gaaatgtcag ctttagcaaa
    10801 aacggtagta caagccttag aaaccttgcc tgatatttta gagcgagact gcggattgca
    10861 gcctaaagat ttgatacgag tgcagcaagt aactgacgat gtacgagacc aaatggcatt
    10921 acatatccaa gaagttacca ctgacacgga gaatgaataa tgtttgctag tgctaaagaa
    10981 attcgacgtg atatagcaaa tgccataaaa gcacctcggc gaatgagagt gtcagaagca
    11041 gtagcggaat atatgcgtgt accacttggc ggaggcaact cggtcaaatg ggataagcac
    11101 accgccgcct atatgttaga accgatggac tgcctcaata gccgagaata cgatgcggtg
    11161 atttttgtcg gcccggcacg aacgggtaaa accatcggct tgattgacgg ctggataacc
    11221 tactcaatta tctgcgatcc gtctgatttc ctgttggtgc aacttaccca agagaaagct
    11281 agcgaacata gccgaaaacg gcttgaccgc acattccgct gttcgcccga aattgccaag
    11341 cggctaagcc cacgcaaaaa cgataacaat gtccacgaca agtatttcag agcgggcaac
    11401 ttgctcaaaa tcggctggcc gtctatcaat gtgctttctt catcggacta caaatatgtt
    11461 gctctgactg actacgaccg ctggccggaa gatgtggacg gagagggcga cggtttctcg
    11521 cttgcctcaa agcggacgac cacctttatg tcagcaggaa tgactttggt ggagagttcg
    11581 cccggtaaag atattgttga tctcaaacat atcccgaaaa gcacccacga ggctccaccg
    11641 actaccggta ttttaagcct ctataatcga ggtgatcgcc gtcggtttta ctggcaatgc
    11701 cccgactgct ccgaatactt cgagccgtca atggcaaata tggtcggcta ccgtgaagac
    11761 agtgattttg tcaaagccag cgaaaacgca cggttacaat gccctcactg ccaaaccctg
    11821 attgcccccg aactcaaacg agagctaaat atcaagggcg tatggctcaa agaggggcaa
    11881 aaaattgatg caagcggtca aattagtggc gaaagtcgca aatcccgtat tgcctccttt
    11941 tggcttgaag ggccagcggc agcctaccaa acgtgggcgc agctcaccta caaactgctc
    12001 aatgccgaac acgaatatga aatgactggc agtgaggaaa ccctcaaagc ggtaacaaat
    12061 accgactggg ggttacctta cttaccacga tctgcattag aacaacgccg ttctgatgag
    12121 ttgatggaac gccgagaaga agtagaagag aaaactgtac cagctcaatg tcgctttatt
    12181 gtggcggcgg tggacgtaca gggcggtaaa aaccgccgat ttgtggtgca aatggtcggt
    12241 tacggtgaaa atggcgaacg ctggttgatt gaccgctata acatctcaca tactttgccc
    12301 gacagtgatg gcgtgattga aaaaattgac cctcgtattc ccgatgattg gtacatactg
    12361 atttcggacg tactagaaaa acgctaccca cttgcccaca atccaaacca ctttatgccg
    12421 attttggcga tggcggtgga cagtggcggt gaagatgggg taaccgataa cgcctaccaa
    12481 ttttggcgca aatgccgccg agatggctat gcgaaacgag tctatttagt caaaggcgac
    12541 agcaccaaac ggcaaaagct gattaccaaa acttaccccg acaataccgc acgaagtgac
    12601 cgccactcct ccgcacgggg cgatgtgccg ttgtacttgc tccaaaccga ctacctcaaa
    12661 gatcggatta acaacgccct gttccgtgat acggtggggg cgaactacat ccacttcccc
    12721 gactggctag gtgaatggtt ttttaacgaa ttgacctacg aagaacgagg agcggacggc
    12781 aaatggcgga aaccgggcaa aggcaataac gaagccttcg acctgttctg ctatgcccac
    12841 gccattgcaa ttttacgagg ctacgaacga atcaaatggg gcgatgaaaa cgatgtgcca
    12901 agctgggcaa aactgcccga catcaaccct aatatcattc gggaaacgcc aagaacggct
    12961 gaacaagcgg tcgaaattga agaaaaatcc gaaaaaccac aacctaaacc caccaaagca
    13021 aaaagcagtt ggttaagtgg tggcggtaga aaaacaggag gctggctatg agcctttaca
    13081 ccatagacga gcttaaacaa aaaatccgca cgcttgatga aaaaatcgaa accgcccaaa
    13141 gccaagtgag ctttaatggt cggtcggttt catatcaagt ggcggaacta accaaacagc
    13201 gtgatcgcta tcaaacaatg ctggatgaac aacttgccca aagcgggcaa cgcaccaaac
    13261 agcatcgaat taaatatgcg aggtttattt gatgaacttc ctcgaaaaaa ccattgccac
    13321 cctttccccg aaatgggcgg caaatcgctc tcgaaatcgc tatgtgctaa atgcctatga
    13381 ggcggcacag cctagccgaa cccataaggc aagccgagag agcaaagggg caaactctac
    13441 tgttcgccaa agtgcggtca gcctacggga acaagcccga gccttagacc aaaatcacga
    13501 tattgtgatc ggcattctcg acaaaatgga agagcgggtg attggctcaa aaggcattca
    13561 tattgaaccg cagccgctga cgttggcagg tgaagtccac gaagaattag ctgggcaaat
    13621 ccgaaagctg tgggcggaat ggtcggtgaa acctgatgtg acgggattat acactcgccc
    13681 attgctagaa cgaatgctac ttcggacttg gctacgagac ggcgaagtct ttgttcagtt
    13741 ggtgaaaggc aaggtggcag ggttagaaca cggctcacag gttgcttttt cccttgaagc
    13801 cttagagccg gattttgtgc caatgcaatc cgatgaggca aaaaacggct tggtgcaagg
    13861 cgtgtttctc aatgcgtggc ggaagccgac tgcttaccaa gtctatttag acaatccgca
    13921 agaaagcaac ggaatgtacg gcaaagtgaa aaccgtgccg gcagacaata tgttgcacct
    13981 cgcctttcgc aagcggttac atcaaattcg tggtgtaagt atgttgcacg gtgtgattgt
    14041 gcgattggcg gatctgaaag aatacgaaga gagcgaacga gttgccgctc gcattgccgc
    14101 cgcaatgacg atgtacatta aaaaaggcga tgcggcactg tatgatgacg acagtaacag
    14161 cgatggcgaa cgcctgtttg atattgcccc cggtgcggtg attgatgatt taaaaccggg
    14221 tgaagacatc ggcttaatca attccaaccg cccgaatgtg aatttggaaa ctttccgcaa
    14281 tggacaactg cgagccactg ccgccggtac tcgttcgagc tattccagca tcgcccgaga
    14341 ctacaacggc acttactctg cacagcgaca agagctggtt gaaagttttg aaggttatgc
    14401 ggtgctacaa gatgcgtttg tggcggcaat cagccgcccg atttatcggg aatggctcaa
    14461 aatggcaatc gcctcacaag cgatcaaact gccgcctgat attgaccaaa actctctctt
    14521 taatgcggtt tattcaggtc ctgtaatgcc gtggattgac ccaatcaaag aggcgaatgc
    14581 gtggaaagaa cgaattaaag gcggtttagc caccgaaggg caagcaatcc gagccagtgg
    14641 caataaccca gccgaagtca aacggcaacg aattgtcgag atcaaagaaa atcaagccga
    14701 agggctaaaa tttgataccg atctcaccaa ttacggtggg caggataaat ctttctccaa
    14761 tcaaaaagat gaaattaacg aagatgaata acaggagggt gcgtgaaaac gtggttcaac
    14821 attcaagcca aagcgaatga caccgccgaa atctcaattt ttgacgaaat cggcttttgg
    14881 ggtgtcaccg cccaacaatt tgctaaagac ttaaaagccc ttggcaacaa tttaaaacaa
    14941 atcaatctgc atatccactc gcccggtggt gatgtgtttg acggcattgc catttacaac
    15001 ctgctaaaaa atcaccctgc taacaagacc gtctatattg acggtcttgc cgcatcaatg
    15061 gctagcgtaa ttgcaatggc aggtgatgaa atcattatgc ctgaaaacgc tatgatgatg
    15121 atccacaaac cgtggggcat tcaaggcggt gatgcggatg atatgcgtaa atatgcggat
    15181 ttactggata aggttgaaag tacactgatt atggcgtatg tcgccaaaac aggtaaatac
    15241 gaaaccgatt tggcagaaat gctgaaagaa gaaacttggc tcacaggcaa agagtgcgtg
    15301 gagcaaggtt tcgccgataa gttagccgaa ccccttgtgg cgatggcttg tatccaatcc
    15361 aaaaaattag aggactacac aaaaatgcct gacaaaatca aaaatatgct attcgcacca
    15421 caaggcaacg caggtgcaaa tccaaaacat gaacaacctc aaacaccacc acaagcgggt
    15481 gaaaatccgc aaaatgtggc aaaacctgat gcaatggtgg cattagccca acgcaatgca
    15541 acgattaaag cgacctttgc cgctttcggc acacaatttg acggcttgct ggcagaatgt
    15601 ttagctgatg tgtcaatgac cgctgagcaa gccaaagaca agctccttgc caaactgggc
    15661 gaaaacacca ccccaagcgt accgcaaaac cacattcacg caggtaacgg taacatcgtt
    15721 ggcgacagcg tgaagcagtc gttaatggct cgtgctggtc aagataccga caaaaccaac
    15781 gcaagagata atgcctacaa tgcaatgacc ttgcgtgagt tggctcgtgc ctcattggtt
    15841 gatcgtggcg tgggtattgc gggctacacc ccaatgcaga tggtcggtct tgcctttact
    15901 cattcgagct ccgacttcgg tcaaatcttg attgatgtgg ctcataaatc cctactcaaa
    15961 ggctgggaaa ccgctaccga aaactacgag cagtttacca cccgtggcac tcttactgac
    16021 ttccgaccgg caaaacgagt cggtttaggt gagtttggct acctgccgga agtgggcgaa
    16081 ggtgaagaat acacctacgg cacaatcggt gatgaaggtg catctgttgc attggcgact
    16141 tacggacaac tgtttagcat tacccgtcag gcgattatca atgacgatat gcatctttta
    16201 actaaaatcc ctgagaaaat gggtgcagca gccaaagcga ctattaccaa actggtgttt
    16261 gccttgatta ccggcaatgc gacagcccaa gacggtaaaa agctgtttga tgctacacat
    16321 aaaaacagct taacgggtgc ggcattgaat gtggaaaaca ttgacaaggc aatccagtta
    16381 atgaatggct ttgtaaacag ccgtggtgaa ccgctctcta ttgagccaga atttatgctg
    16441 ttaccaacct cattgcatac caaagcaaaa cagattcttg gttcgtcaag cgtggaaggt
    16501 gcagacatca acagcggtat cattaacccg atccgtgata ttgtcagccc gattaaatcg
    16561 cctcgcttac aaattgcaga tccgaaatca tggtacttgc tcaataaaga ggcgattgaa
    16621 gtctcttatc tggacggggt ggatagccct tacatcgagc aacagcacgg ctttacggtg
    16681 gacggtgttt cgaccaaagt ccgtatcgat gctggtgtga atgtgattga ctaccgtggt
    16741 gtggtgaaag tgaccaacgc ttaatttaaa caatttaaaa cctgaccgca cttttaacag
    16801 tgcggttttt tattatccga aaaaaggaaa aagagatatg gctaaaaact atattcaaga
    16861 cggcaatacc gtgcgtttaa ctgcaaccaa agcgattact tccggcgatg tcattgtggc
    16921 ggaggattta attgcgattg cggtttctga tgcggcaaaa aatgatgcag tagttggctt
    16981 aactaccggt gtttttagcg taaaagctaa acaagcagat gacatcaaac aaggtgcagt
    17041 gttgtactgg tctgaaacag agggcgcaac tctcaccgca ggctcaaata aacgacttgg
    17101 cattgcgtgg aaagactctg gcacatcatc taatcaagtt gatgtgaaaa tcaatgtcta
    17161 gcccgtttga gcaggcaata gctgccgccg atcaaactat tcagcaaact atgatgagtg
    17221 agtggttgat tggcggcaag ccttaccctg caacttttga tgaagccccc gctattttta
    17281 acggtttaca ctcaaccgat gacagagcta ttcacggcac ggaacgcaca ctgaccctat
    17341 ttcgggcgag tggctacaaa ccgagattgg acgaccgagt gagcggaaat ggcaagaaat
    17401 accttgttaa atcctatcac tttgtcgatc agttaatcgt attgcaattg gagtaaacaa
    17461 tgggttctca agtgaccggt ttaaaagaac tgcaagcctc cctgaaaaaa atagctaggc
    17521 aaaccgtacc taaagcagcc gctcaagcaa tccgtactgt tgggcggcaa gcaatgaata
    17581 aggcggttaa atcggtggcg gcagagattg gcgtaaatca aaagacaatc aaaggtagag
    17641 caaaaatgac ggcaaagccc acgccgtcaa gattgcaagc cacgattaag gttaatcgat
    17701 cacatatgcc gatgattcgg gtactagagc gcaagtccaa ccgcctgtca cccaccaaag
    17761 gcagtattag agttgggaaa cacactgtac aacgtggttt tcgccaacga ctcgccaatg
    17821 ggcgaacgca cattatgtac cggcaaggtc gtaagcgtta cggcattgat gtagcaaaag
    17881 tgccgttatc ccaaccactt acacaagcat ttgatcgaga attgaaaaac tatcccgaac
    17941 aagtgcaagc ggaattagct aaacaaattg cggggaaatt atgaaaatcc acacaaaaat
    18001 ccgaaaagaa gtccttgaat tattacagac taaattgccg gaaattgata atttttataa
    18061 cgggctgcca acttttattg atattgagga ggaacaactt gcagtatcgg tttatctcga
    18121 tgatatcagt ctgcaagaaa taactgtttg taataatcaa tgggacgcca agctcaatat
    18181 tacaatccat ctgaaatcta ccgagagtgc agaagatgaa ttagacgaat gggcggaaaa
    18241 aattcgtgaa attatcgatg attatcccga atttgagcat ttagagtcag tcgcgctctc
    18301 tcagtatcaa tatgagcaag accagaatca gcgtacgtgg cacatggcga cattaatctt
    18361 tgatgttgag tattaaacaa ataatctcta taggagactt aaaatggcaa aaacaacaaa
    18421 agtccaaggc acgaaattta gaattagcgt tggacgtgag gctcaaaaag caattaccgc
    18481 aattaactta aatactgcta ctctaacgat tgcatcctca ggctataaaa aaggtgatgc
    18541 aatcgagctt acaggttgtg gtcaattaga tggtatctac ccggtgttat cagtaactac
    18601 cgatcaggta aaattatgcg aagaagtgaa ctggacaggt aaagatttac cagctaatta
    18661 caccaaagcc aaggcggcac tagtgcaata ctcggagcaa ttctgcgcgg taaaaaacat
    18721 tgaaaaatct gatgatactt tgagtaccga agatgtaaca acggtttgtt cagagggcac
    18781 agaaaccgag ccgggtgaaa ttgaatttgg ttcgatcaaa ctcagtttct tccacaagcc
    18841 tactaccgaa atgcaaaccc gattgcgcaa actcttttac gacaaatcca cctttgccta
    18901 caaactggaa ctaccggata accatggcac gacctatggc gaaggcttta ttgaggccgg
    18961 caacggtttc tcgggcgaag ttaagggtaa gtatgaaggc tctgtatcta ttaagccaag
    19021 caaacgcgac tacttattag tttaattgca aaaaaaccat aaaattcgac cgtttgtaga
    19081 aataacaagc ggtcttttta tatcagaatt ttacaaaagg aacgaacaat gacactgcgt
    19141 gagaaactcc ttgctaataa accaaaattg caacctatcg aaatcaacgg cgagacctac
    19201 tacctgcgtg aagctactgt tggtgatatg aacaagcaga tttttgaaac ccgaagctgg
    19261 ctcattcaac aagctgaaca agaaaatgtt gaattaccgg cagaagatga tgaaaccttt
    19321 gatgaggctc tcaaccgttt tggcgaaaaa taccgccttg ctcaatcggt tgcctaccgc
    19381 ttatgtgatg aaaacggtgt attactgttt aatcctctcg acattaacga tctgaatgcc
    19441 attgccgaat tagacagtaa agtgattatc gactttaacc aagccgtgtc cgccccaaaa
    19501 gactcagcga gcgaagaaag ttccaaataa ccctttcgct cgcactgggt aaaacacttg
    19561 aagaaatcga acaaatgccc gaacgccact ttgccgaata tcggcttttc taccaagaac
    19621 agcctttcgg cttatggcga gacgattacc gcaccgccca acttgcccac ttaaccgcaa
    19681 tgttcaaccg tgatccgaaa ggcaaagctc caaaattagc ggagtttatg ccgtttttta
    19741 atcggacaga tgaggtggag gaaatagaag atgatggtgt ggcagattat ttggcgaaaa
    19801 ggtaaagaaa acagcttgaa attccttgct cttggatata tatttcctca aagagaagga
    19861 gggaagtatg tttgaagcac tagaccgttt tatgattaat acgattaaaa ttgcaattat
    19921 tgtatttttg gtcgcttgca tctttattag tatgccgaac tggtggatag cttctatctt
    19981 agtacttttc ccgactgcct atttgcttga taaatactgg tggcgaaata ccgaaatgta
    20041 tcgccgcaaa atggataaat tagttgaaga gatgaaaaat aatcgtcaaa tctaaattaa
    20101 catcaattct agcccgcttt atgcgggctt tttatttggg gtgaaaaagt gagtagctca
    20161 aacagattaa ctattacctt agatgtagaa aattctaagt tactagctgg attcgcacaa
    20221 tctgatacag tagcaaataa gtttgctcgc agcttcattg tgaatatgga tcaggctcag
    20281 caaaaagcac gtcaatttgc tgatagatcg acaaaatatc taaaaaatat tgaacaagca
    20341 gcaaataatc ttaatagctc tgtatcaaaa gaacgttttt ggcaaattgc aaatcggttg
    20401 caattcccga caagtaaagt gattagttat gccaatagct acaccgaact cggtaataaa
    20461 ctcaaattag tcaccgaaag tgaatcccaa cacgcccgag caatggcggc ggtgtatgat
    20521 atttcgctca aaaccgccca atctacacag gcaacctctt cggtttatca gacctttgct
    20581 caaaatgcaa aacagctcgg tatttctcaa aacgatgtgg caaaactaac ggaaacggtg
    20641 gcgaaatcag tggcgatctc tggtgcaagt tcggcaaccg cttcaaacgc cctagtgcaa
    20701 ttcagccaat cgttgttaat gggtaagctc aaagcccaag aatttaactc attgatgacc
    20761 caaacaccgt ctgttattca ggcgattgcc aaagggttag gaattactac tgccgaactt
    20821 aaagcaatgg tggataacgg tgagatgtcg gctgaaaaaa tgattgaggg gctaaaaaaa
    20881 gcagagagtt atgtaaacgg acagtacgcc aaaacaacca ctacaattag tggagcaatg
    20941 caaaatcttt caactgcggc tgaaaagtgg gttggcgaaa tggatagtac cgttggtgtg
    21001 tcacagaaag ccgttgccgt tctgaatgcg ttaaatgcta attttgacac ggttgctaaa
    21061 acggtacttg tcgccggtgg agcctttgct ctttttaaag gctatatgaa tgtacgccca
    21121 tttatcgagt caagagcggc tatttatgaa aatcaaaaag caattattgc tgaaaatcaa
    21181 gcccgccaac aggcaatggc gattcgtcag caagagttag taggactgtc tcaacaaacc
    21241 gctttaaaac tggaacaaac attacaaacc tacaatcaaa ataaagccga attagaggca
    21301 attgcagtta aacagcagaa aattacaaaa gaacgggaag aaatcgcagt ggcaattcaa
    21361 gcggctgcaa ctcgttcgga aagattagcg ttatccgccg aattacaagc cttagatcaa
    21421 aaggaaatca cgctcggaca acagaaaata gcggttgaac gccagcaaat tgcagcaaag
    21481 caggctctca aagtggcgta tgctgaaaat gcggttgctc aaagtgcaat gagtgcaggt
    21541 atgatcacag caaccacaac aaccagagca atgaatggtg ttttagcggc tgcaagagct
    21601 gaattgaatt tactgaaagc agcagcactg tctaacccat taatgacact cgcaatggtg
    21661 gctacaactg ccggtactgc cctttgggga ttttttgaaa gtcaaaaagc agctcgtgaa
    21721 gaagccctcc gctacgctga cagcttagac tctgttcgag tcaatatcga aaaaatgact
    21781 aaggctcaaa ctgatgcaga aatggttaaa ttgtctcgtt cgatcaaaga acaggaaaca
    21841 gcattagcta atctgaaaca acgccaagct gaacttacac gagaaataga gcaaggagct
    21901 acggtttatg aggatgcgtt tgccggcact gtgcgtgtta cgatgagtgc ggaagagctt
    21961 gcgaaaaaac aagaagaatt aacattagtt actgctgatg ctgaaaaagc tcaaaaaacc
    22021 ttgaattcta gcttagaggc tcaagaagcc ttaaaagctc atattccgat tgctaaccta
    22081 cgagaggaat ttgttaaatt atatcctcac attgatgaaa gccaaatcaa agttgatggc
    22141 ttaaatattg ctatcggtaa ttttaccgtc aaatcaccgg agatggttat tgctactaat
    22201 aacattgcta atgctcttgg tggcgttgcc ggagaggcta tgagagcggc tattctagtc
    22261 gctaatctgt caagtatgaa gctggatgct aacggtgctg caattatcga tcctaaacat
    22321 cttgaaaaaa tcgagcaaat tgaacgtaac aatgcgattg gttcggctaa aggtaaagat
    22381 aaaatcgcat tgcaagtaaa agacgctctg attaaatcag gtatgaaaga gggcgatgcc
    22441 ggatacgagc gactaaaagc agcatatgag caacaattta cgttgcaaaa tgcccctaag
    22501 ggcaagggtg gaaaaaccaa agccgaaaaa gaggcagaaa aagcggctaa aaagcaacaa
    22561 agttatcaaa accaagtggc ggagatgaca aatcgtttgg caggcttaaa agccaatgct
    22621 tcagatatcg cgattttcgg gcaagtctcc gactaccaag aagtgcgtaa actgactgaa
    22681 gatattgcga tcaatgccga aaaatacaaa ggctacggtg agcaaggtgt cgcaaggctt
    22741 aaagagttag ccagtcagtt agagtccgcc cagcaacaag tcgctatttc tcaatttgcc
    22801 tacaatggcg gtgagaaact caaagcaatg gagtttgaat taaccttgct cggtaaaacc
    22861 cgccaagagc aagagttaat gcagtacaac cacgaattag atcttgaagc agctcggctc
    22921 aaaatcggta tggctgatga aaatattgcg aagttagacc aggaaatcgt taagctaaaa
    22981 gaacgtcgag cggaaatcca agcacaagca gaacaagctc gtggcagctt taaacaaggt
    23041 atgttagatg gtttgaataa tatcgaagct gatgtcagca atgtagcggc aaatgttgcc
    23101 aatattactc aaaatacctt tgacggtatg gcggatagct tgactaattt tgtgatgaca
    23161 ggtaaagcgg atttccgtag ttttgctaat tccatattat ctgatcttag caaaatgatt
    23221 atcaaaatga tgttatttaa tgctatcaaa caaggtgcaa ctatgttgtt tggctaccaa
    23281 ggaccgacta caacggtaaa tggtaatgct gtttatgggt taggtacttg ggcaacaggt
    23341 ggctacaccg gcgatggtgg taaatacacc ccagcgggta tcgtccacaa aggggaatac
    23401 gtcatcacca aagaggcaac cgctcgtctt ggtttggatt acctaaacta tcttaactat
    23461 ggcaaacgag ggttttccag cggtggtggc gttgctgtgc cgagagtgcc atcctcatct
    23521 tatcaaccta aatcagctca aagtaatatc agtgttcagg tgattaataa cggagagccg
    23581 gttgatgcaa aagtcagcca gaaacagcaa ggcgatcaaa tgcaaattac cgttgagtta
    23641 atgcgtgaaa ttgcccgaca ggaggcaaac ggtatgatcc aaaacaactt cagagctgga
    23701 ggagtatttg cctaatggaa acattaaaat ggtgtgttcg caccgattta gcggttgaaa
    23761 atacccctga aataatggaa gtcaaattcg gcgatggtta tacccaacgc tcgcccaaag
    23821 gcttaaacaa tttattacga gcctatacag gcacaatcaa agtgaaaaag ggggaacatt
    23881 tagcggtgga ggcatttttt gccaaacatc gtggtgtttc ccctttttac tttaaagatc
    23941 cgtacacaca acaaaacaaa aaggtagtgt gtagcagttg gcccgcaaaa atggttggct
    24001 taaaccattg ggaatttagt gtaaccttta aggaagtacc ctaatgcctg ttagcatatc
    24061 caatcaaatg aaactcgacc ttgccaaact tgagcaaaat gcaatgcttg atttgtacga
    24121 ggtggactta cgcacattaa aggataaaag cggtaatgct ggttc
//
LOCUS       DANLZC010000020        23544 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.020, whole genome shotgun sequence.
ACCESSION   DANLZC010000020 DANLZC010000000
VERSION     DANLZC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23544
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.020"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            70..1215
                     /gene="lldD"
                     /locus_tag="PXE44_000634"
     CDS             70..1215
                     /gene="lldD"
                     /locus_tag="PXE44_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HDL5588175.1"
                     /translation="MIISSANDYRRAAKRRVPPFMFHYADGGSYAEQTLRRNVSDLED
                     IALRQRVLKDMSQLDTGIELFGEKLSMPVTLAPVGALGMYARRGEVQAAKAADNKGIP
                     FTLSTVSICPIEEVAPAIKRPMWFQLYVLKDRGFMKNALERAKAAGCSTLVFTVDMPT
                     PGARYRDMHSGMSGPYKDIRRVLQAMVHPFWAWDVGIHGKPHTLGNVSNYMGKPIDLN
                     NYIGWLTDNFDPSISWKDLEWIREFWDGPMVIKGILDPEDAKDAVRFGADGIIVSNHG
                     GRQLDGVLSSAKALPSIADAVKGEIKILADSGIRNGLDVVRMLALGADCTMIGRSFVY
                     ALSAAGQAGVENLLDIFLKEMKVAMTLTSNAKISDIGRDALVDLSNL"
     gene            complement(1269..1538)
                     /locus_tag="PXE44_000635"
     CDS             complement(1269..1538)
                     /locus_tag="PXE44_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588176.1"
                     /translation="MLSEERFGFFYIFIFIYFSLLSLSIGYIRPELRTTYDYLEIENK
                     RYYVLSIHRNNIYVLGEKTKDNDEFLFFNQDTLKYYRINITKMPN"
     gene            complement(1613..1999)
                     /locus_tag="PXE44_000636"
     CDS             complement(1613..1999)
                     /locus_tag="PXE44_000636"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588177.1"
                     /translation="MTNQKFINSSLTLAFFTLISFSTAYCYAWGIAAFHGYPWWHVEV
                     GNAGIARALAYVFGTFLTIFLFYLIGYALVNKVFKLHYFKYLGWLRVSVLVTIFSLPV
                     MISFYLFIGKVPMYLHLLYLGTTTSI"
     gene            complement(2252..3082)
                     /gene="fghA"
                     /locus_tag="PXE44_000637"
     CDS             complement(2252..3082)
                     /gene="fghA"
                     /locus_tag="PXE44_000637"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDL5588178.1"
                     /translation="MQQFENYRCFSGEQQVWQHFSASLNCEMKFAIYLPQSAKTQKLP
                     VLYWLSGLTCTEQNFITKSGFQRYADEHHLIVVAPDTSPRGVNVANDEAYDLGQGAGF
                     YLNATQGEWTTHYQMYDYIVSELPTLINAHFPTNGKQSIFGHSMGGHGALTIAFKNPG
                     QYQSVSAFAPIVAPTQVPWGQKAFLAYLGEDQTAWKAYDSVALLEKYRPSLPILIDQG
                     SQDSFLSDQLKPEIFCETADRLGIPYQFNLRDGYDHSYYFISSFIGEHIAFHAKHLKD
                     "
     gene            complement(3162..4220)
                     /locus_tag="PXE44_000638"
     CDS             complement(3162..4220)
                     /locus_tag="PXE44_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDL5588179.1"
                     /translation="MAYPIKSYATFSATTPLAPHSFERRELLEDDVLIDILYCGVCHS
                     DLHMARNDWGWTQSYPLVPGHEIIGRIIKVGKNAKKFQIGDLVGVGCMVDSCRECSPC
                     QHGLEQYCEVGNIGTYGGIDRFDGTPTQGGYSTCVVVSEDFVLKVSENLDTKAVAPLL
                     CAGITTYSPLRHWNVKAGSKVAVVGLGGLGHMAVKLAKAMGAEVTLFTRSLGKADDAY
                     RLGASRVVLSTDEKQMADVANTFDLIIDTVPYTHDLKPYIPTLALDGTLVLVGLVGEL
                     EQTINTVPMILGRRSISASVIGGIQETQEMLDFCAEHNIVPDVEMIDMQNINDAYERM
                     LKSDVKYRFVIDMGSLKG"
     gene            complement(4407..5342)
                     /locus_tag="PXE44_000639"
     CDS             complement(4407..5342)
                     /locus_tag="PXE44_000639"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03571 family LLM class oxidoreductase"
                     /protein_id="HDL5588180.1"
                     /translation="MNTLSPIFSQVFGQNQLTLGLIAPFMGYQNHIPDNLTTIADNAR
                     LAENLGFSALWVRDVPFYDPHFGDVGQGLDPMVTLGFLSAHTQNIALGTAGLIAPLRS
                     PIHIAKMATSVDSLSHGRFLLGLSSGDRPVEYPAFGADFASRSERFRESIDLINTLTE
                     QDFPHFKGKHYADLTDNLDLLPKPKHRLPIVAIGRARQEMDWLANVPDAWIWHGVNPA
                     DTANIINTLADLRQKDTPTAFGYAQFVEVLDDKNAKAQLFNNIYLRGGAKGLADFWAK
                     EQEKGVKHLALNLKPTQRPADEVLTELAEYALTQF"
     gene            complement(5622..5933)
                     /locus_tag="PXE44_000640"
     CDS             complement(5622..5933)
                     /locus_tag="PXE44_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5588181.1"
                     /translation="MSETEKQAINAPNAVMNGYLTMHYPDWFKPDGIYFNDGAFESFE
                     SSHKLTKDGKIRLVPTAGHTLGHLAVVVDMGEHYILIGGDASYSEQDMLAGNIDGVCN
                     A"
     gene            complement(5940..6332)
                     /locus_tag="PXE44_000641"
     CDS             complement(5940..6332)
                     /locus_tag="PXE44_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5588182.1"
                     /translation="MIKVHCLTTGWVQIKIHHQVARFFARPARVLDVLTDKKWSPKLP
                     IGCWLIEHDDGLILVDTGESSRANDKGYQPWWHPFMQFCERRGVKKHEEVGEILKANG
                     FDPLRIDTVVMTHMHGDHAGGLPNFPNS"
     gene            6464..7024
                     /locus_tag="PXE44_000642"
     CDS             6464..7024
                     /locus_tag="PXE44_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL5588183.1"
                     /translation="MNDLRVIKTHLAIQNALIELLDEKLFEKVQVQEIIEKALINRTT
                     FYRYYSGESDLVGKLIKEVKAEYQDLLKKRFESGNLLRFMQTIGNRLLEKRRLILALW
                     QIKTPRHHLYDDMHMLLKNAFIAHAKRITSDDKNWAEKNWDYQAHIFATIALESHKYY
                     FEQGKLLPIPQVFEEWIEMVQVLKSV"
     gene            complement(7090..8079)
                     /locus_tag="PXE44_000643"
     CDS             complement(7090..8079)
                     /locus_tag="PXE44_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL5588184.1"
                     /translation="MSKQIQFTKTGSPDVLQIVDVAIPAPKAHEVQIQIQAIGLNRAE
                     MMYREGAYVIDPVFPATLGYEGAGVIVAIGEGVNEVAISDKVSIIPSFMFTEYGTYGE
                     IVNMPKHAVVKHPDNLSMEQASASWMAFVTAYGGLIEFGKVQKGDFVVLGGATSSVGL
                     ASIQIAKMQGATVIALSRTHAKGNVLLEKGADFVIATSEDDVTAKLFEITNGKGVNVV
                     FDPVGRKEAAKIINAMAQDGRYIIYGALSHDDIAVPVFPILGKHLTVRGYELFEITTV
                     PEKLAQAKTFVYDGLASGQLRPEIDKIFAFDEMVKAHEYMASNQQIGKIVVKV"
     gene            complement(8293..9210)
                     /locus_tag="PXE44_000644"
     CDS             complement(8293..9210)
                     /locus_tag="PXE44_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5588185.1"
                     /translation="MTFIIGILAGVLIAFQSAINARLRMHVGAAFLTSFVSFSVGLLF
                     LLGLSLIMQQTPAFSLDNLGNVPLWAWTGGLLGMVGLTANVLIFPKLGGVQTAVMPIL
                     GQVLMGVLIDTFAWLNSPYIAFTASRLFGIGLVLFGVWIAVVLPHLSGLKRKENANLW
                     LWRAVGILGGTALATQAAVNGEFGRQPGSSLGAATVSFGVGAIGLLLVVLFYEKSLPK
                     LCQPTEGKPFWIWSGGILGSLFILAGVWLVPQIGTGSVVMLVLSGLICGSLLVDKFGL
                     FGITQKPILPLQIIGVTLLLAGVAFIRLL"
     gene            complement(9346..10005)
                     /locus_tag="PXE44_000645"
     CDS             complement(9346..10005)
                     /locus_tag="PXE44_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5588186.1"
                     /translation="MQFFNKEDVINAFHYRASTRSYDGSRKIPAEDFNYILELGRLSP
                     SSVGSEPWQFLVLQNPELRQKIKPYCWGIPTMETSSHIVVILAKKNARYDSQYFSEIM
                     DRRGLQGEAREKAMAVYKKFQEKDIGILDSERSLFDWASKQTYIALGNMMTGAALIGV
                     DSCPIEGFAYQEVNDILAAEGLFDPSEWGVSVMCTFGYRDKEIRQKARKDFETVVKFI
                     E"
     gene            complement(10190..11254)
                     /locus_tag="PXE44_000646"
     CDS             complement(10190..11254)
                     /locus_tag="PXE44_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HDL5588187.1"
                     /translation="MAKFRKLFTPFNIKNLALKNRVVMPPMCQYSATNGVPNDWHFVH
                     YTSRAVGGVGLIIVEMTNVAPNGRITPNCLGLWNDEQQQAFKKLVDSVHAQGAKIGVQ
                     IAHAGRKAQDEPDAVAPSAIHYGELDYAGQNLITPRELTTDEVKALVQAFKDSVKRAV
                     EAGFDTIEIHGAHGYLIHQFYSPKSNQRSDEYGQDKMLFGEQVIQAAREVMPSEMPLI
                     VRISAQEYGENGFGLDYGLQVAKRFADAGADVLDISGGGDGVLHAGNHPEFYAGYQVD
                     FARQVKLATGKPVIAVGMLDNPAVGDHILGMGDADLVAVGRALLRDPYWVLNAQYDQN
                     AADSSEMQFVPKQYERGLFM"
     gene            11345..12253
                     /locus_tag="PXE44_000647"
     CDS             11345..12253
                     /locus_tag="PXE44_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5588188.1"
                     /translation="MSDIRTLDFNLLKAFVVLLDECNVSRAAQRLSITQPAMSGILNR
                     LRESFNDPLFVRVQHGMQPTDRALQLGQTARNILQDISSMLQPPILEPEHLNMTLRIA
                     AMDYVQQIIALPLILRLRRLAPNVKVALLPVQGQNIKTLFEQKKIDLALVSRQHLSTD
                     TLKTVLYEERYVCAMSKTHPMAQTPLSLDQFCNLPFAMLSYNGGEFSGATDLALQKIG
                     LQRKVMVSVNHISLLPQLLQGSDLVAVLPERLAKTLPNVYLQPPPIEVEGFTMMMAWH
                     ERTEQDTAHRWLREVLQGVVKPKQAL"
     gene            complement(12323..12874)
                     /locus_tag="PXE44_000648"
     CDS             complement(12323..12874)
                     /locus_tag="PXE44_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5588189.1"
                     /translation="MAKQIAVLIGSGSKTSFSHLTVRHLQKMAPASIQLNIVDIADLP
                     LYDRDLDENSPAQYTRVREAVAVADGVLLVTPEHNASFSAMLKNAIDVVSRPMGQSKW
                     IGKPAGIVTVGASPAGGVRAADQVRTITSGSFVNMPTLPFALNVGNLFTGVFDEQGEI
                     ASETLKSSMQNFINAYAQFVEKY"
     gene            13066..14427
                     /locus_tag="PXE44_000649"
     CDS             13066..14427
                     /locus_tag="PXE44_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL5588190.1"
                     /translation="MLFLNKIKQLFIFAFTLVLSACSLVVYKPVETIKQVNVERGYRQ
                     KNVIAESAKDGNIVVIMFSGGGTRAAALGYGVLEEFKKVRVKETNKGNTLLENIDIAF
                     GVSGGSILATYFALEGKDIIPKFADRFLKANFQKDIITEVFSLSNLPRLSSPQYGRGD
                     LLQERLNFALYHGKKFGDLVKNRKGPFAVISATDMNIGQKLTFTQETFDGLCLNLNDL
                     EISRAVAASSSVPLIFAPLTLNNNGGNCHFSMPQEFSAREKAIDGLITKNIEETNHIL
                     SLYQDSKERPFIHLVDGGLTDNLGLASVLEISDVMGIEEMYQYIHQLRVKNIVVINVN
                     AQNEVSSEIDKTANVPGVADVVNTIVNVPIDRTTQINLQRYRQFTDGWNEYANKQPSK
                     IEMHFVSLGLKELPDGDLKKDVLNIATSFYLPEEDVDKLREAARVLLQQSQEYNAALK
                     TLQ"
     gene            complement(14475..14972)
                     /gene="rraA"
                     /locus_tag="PXE44_000650"
     CDS             complement(14475..14972)
                     /gene="rraA"
                     /locus_tag="PXE44_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDL5588191.1"
                     /translation="MRIDTSALCDLYSDQVDVVEPIFSSFGGASSFFGKITTVKCFES
                     NGLIAEVLEEEGEGRVLLVDGGGAVRRALIDAELAQLAVDNGWEGIIVYGAVRQLDIL
                     ERLDIGIHALAPIPVGADNTDIGEVDTPVNFGGVTFFPEDYVYADLTGIILSQELLDL
                     NDISE"
     gene            complement(15070..15972)
                     /locus_tag="PXE44_000651"
     CDS             complement(15070..15972)
                     /locus_tag="PXE44_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDL5588192.1"
                     /translation="MTEKVTETAPNIDVVAETEKVIHKVSNMNIETMLNEWIIPYGTK
                     ILLAIAIFVVGKFLARLISRLLGKAALKSTKDEMLQSFIVSISYFLLLLIVVIASLSQ
                     IGINTSSLVALIGAAGLAIGLSLQNSLQNFAAGVMLLIFKPFRKGDLIETGGMTGVVE
                     QMGLLVLELRTGDNKTVLLPNGKVFSDSIINYSNNPTRRIDFTFDISYESNLKEAKEI
                     IQRILDANTYVLKDPAPVVAVGALAPHSVQLVVRPWVQTPNYWAAYWEIIEQVKLAFG
                     EAGISIPYNQMELHIKSGSLENKA"
     gene            16081..17043
                     /gene="rluC"
                     /locus_tag="PXE44_000652"
     CDS             16081..17043
                     /gene="rluC"
                     /locus_tag="PXE44_000652"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDL5588193.1"
                     /translation="MTTEKVISAGVQFFTISEDEAGQRLDNFLLAKLKGVPKSLIYRI
                     VRKGEVRVNKGRNKPEYKLQPDDLVRVPPVRVAEKNEAPISTKLNKVAELESQILYED
                     ESMLVINKPSGIAVHGGSGLSFGVIEALRALRPQARFLELVHRIDRDTSGILLVAKKR
                     SALRSLHEQLREKVVQKDYLALVRGQWQAHTKVIKAPLLKNELASGERIVRVSEEGKP
                     SETRFSIEERYANATLVKASPITGRTHQIRVHTQYAGHPIALDDKYGDAEFDSKMKEV
                     GLQRLFLHAHAIRFEHPKTGEEMVITAPLDKTLKGVLAKLRANY"
     gene            17152..18009
                     /gene="folP"
                     /locus_tag="PXE44_000653"
     CDS             17152..18009
                     /gene="folP"
                     /locus_tag="PXE44_000653"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDL5588194.1"
                     /translation="MQIHFKNFQKNDRLLDLTTPQIMGILNFTPDSFSDSGKFFSLDK
                     ALFQAEKMLADGASIIDIGGESTRPNAAIVSLEQELERVVPVVEAIRSRFDCLISVDS
                     SKAEVFKESAKVGMDIINDIRALTEPNALAAAVELGLPVCLMHMQGNPQTMQQNPHYD
                     DVLEDVADFLNQRIFECITAGIPKEHIILDPGFGFGKSVKHNYQLLKHLKAFVDSGFP
                     VLAGLSRKSMIGAVLDKPVEQRLLGSVVGALLAAQNGAKILRVHDVAETADALKIWQA
                     MLESDNEEK"
     gene            18009..18356
                     /locus_tag="PXE44_000654"
     CDS             18009..18356
                     /locus_tag="PXE44_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDL5588195.1"
                     /translation="MTTLVYGIKNCDTVKKALKWLEDHNQNPQLHDYRVDGLNADWLV
                     EMEAKFGWENLVNKRSTTWRNLDEEIKNNLNRESALKVLTEQPTLIKRPIVIKEDIAL
                     IGFNEKEYVAAFN"
     gene            18486..19634
                     /gene="dapE"
                     /locus_tag="PXE44_000655"
     CDS             18486..19634
                     /gene="dapE"
                     /locus_tag="PXE44_000655"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDL5588196.1"
                     /translation="MKNEIISLAQNLIRRDSISPQDKGCQQEIAQRLEKLGFNIEWLP
                     FGDTLNLWATHGSENGKTEPCIVFAGHTDVVPVGDESQWAYPPFSAEIVDGMLYGRGA
                     ADMKGSLSALVVAAETFVQHHPNHKGKVALLITSDEEAAAKDGTVKVVETLMARNEAV
                     HYAVVGEPSSSKVFGDVIKNGRRGSITADLYIEGIQGHVAYPHLAENPVHTSLGFLTE
                     LTTYQWDNGNEFFPPTSLQIANIKAGTGSNNVIPGELYVQFNLRYCTEVTDEIIKAKV
                     AEMLEKHGLKYRISWNLSGKPFLAGNGKLVEAALQAVENVTKITPRLDTGGGTSDGRF
                     IALMGAEVVEFGPLNATIHKVNECVSADDLAHCGEIYYQILEKLLATA"
     gene            19745..21079
                     /locus_tag="PXE44_000656"
     CDS             19745..21079
                     /locus_tag="PXE44_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL5588197.1"
                     /translation="MQLYSYICLLFGLAMFISFFTRKLNENIQTTIAITASALVGSLL
                     ILTFGSLGWFNVDKLAISIFEQLDFKSFLLNGMLGFLLFAGSLGIKLPLMKEQRREIA
                     VYALFSTLVSTFLIGALIYGVARLLGLELGFVYCLLFGSLISPTDPIAVLAIIKSLKA
                     PKRLSMHVEGESLFNDGIGLVIFTTIFAVAFGGQEPNLSGITGLFLQEAVGGIVFGLL
                     TGFVAHKFISATDDGSLEILITLTIPTVGFVIANYLHVSGALTMVVAGIMIGNWTRRS
                     GFSEQSQKYLDHFWEMIDHSLNYLLFLLIGLSTLLVDFTFIGGVLMLAAIPICLLARY
                     ISLWIPYQVLKRFRTYNPYTLKIMTWGGLRGGLALAMALSVPAGAAVVSSSEGNLDVR
                     DLILLMTYAVVTFSILVQGSTVEPMIKKSKLVDPRKVALNKLGAPDDVIPHI"
     gene            21186..21695
                     /locus_tag="PXE44_000657"
     CDS             21186..21695
                     /locus_tag="PXE44_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDL5588198.1"
                     /translation="MLFTGLLCGFLLGFVMQRGRFCITGAFRDLYVTKNSRMFVALLI
                     AITVQSIGIWLLYEAGSFSSPAEDLPLLAVIIGAFLFGIGIIYAGGCATGTWYRAGEG
                     LIGSWVALVIYGLFSASMRTGVLAPLNQELKSNVIQHRTIYETFGISPWVLVFILSVI
                     TLALSAHSI"
     gene            21724..21945
                     /locus_tag="PXE44_000658"
     CDS             21724..21945
                     /locus_tag="PXE44_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL5588199.1"
                     /translation="MNVRLPASGLVCPFPLVEAKAAMAKLNKGDSLTIEFDCTQATEA
                     IPNWAEEEGYEVTDYQQLGDALWEITVVK"
     gene            complement(22010..23518)
                     /locus_tag="PXE44_000659"
     CDS             complement(22010..23518)
                     /locus_tag="PXE44_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDL5588200.1"
                     /translation="MSKCPVTHLTMNNGAPVVDNQNSLTAGPRGPLLAQDLWLNEKLA
                     NFVREVIPERRMHAKGSGAFGTFTVTNDITKYTRAAIFSQVGKKTEMFARFTTVAGER
                     GAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNMRS
                     ATNNWDFWTLLPEAFHQVTIVMSERGIPASYRHMHGYGSHTYSFINAANERFWVKFHF
                     HTQQGIKNLTNEEAAAIIANDRESHQRDLYEAIERGDFPKWKMFVQIMPETDADKVPY
                     HPFDLTKVWPKSDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGISVSPDRMLQ
                     ARLFNYADAQRYRLGVNHHQIPVNAPRCPVHSNARDGQGRVDGNYGSTIHYEPNSFGK
                     WQEQAQYAEPPLKINGDAAHWNYREDDADYFSQPRVLFNMMNDEQKQALFNNTAAGMG
                     DALDFIKYRHIRNCYACDPAYGEGVAKALGMTVEDAIAARATDPALGQPGLL"
BASE COUNT         6689 a         4964 c         5186 g         6705 t
ORIGIN      
        1 tagtaattct tatttttatt ttaaagtaga atagccgcac ttttttctta atgatttatg
       61 aggttttaaa tgatcatttc atctgcaaac gattaccgcc gtgcggcaaa acgtcgtgtt
      121 ccccctttta tgttccatta tgcggatggc ggttcttacg ccgagcagac cttacgtcgt
      181 aatgtgagcg atttggaaga catcgccctg cgtcaacgtg tgttaaaaga tatgtcgcaa
      241 ctagacaccg gcatcgagct gttcggcgaa aaactgtcta tgccggtgac cctcgcccct
      301 gtcggtgcat taggaatgta tgcccgccgt ggcgaagtgc aagcggccaa agccgcagat
      361 aacaaaggta ttccgtttac cctttccact gtctctattt gcccgattga agaggttgca
      421 ccggcaatca aacgcccgat gtggtttcag ctctatgtgt taaaagatcg gggctttatg
      481 aaaaatgcat tggaacgagc caaagccgcc ggttgttcca cgctagtgtt taccgtagat
      541 atgccgaccc ccggcgcacg ttatcgtgat atgcactccg gaatgagcgg cccatacaaa
      601 gacattcgcc gtgtgttgca ggcaatggtt cacccgtttt gggcgtggga cgtgggcatt
      661 cacggtaaac cccacacgtt aggcaatgtg tcaaactata tgggtaaacc gattgatctg
      721 aacaactaca tcggctggct gaccgataat ttcgacccat caatttcgtg gaaagattta
      781 gagtggattc gtgaattttg ggacggcccg atggtgatca aaggcattct cgacccagaa
      841 gatgctaaag atgccgtaag atttggtgcg gacggtatca tcgtctccaa ccacggtggt
      901 cgtcagttag atggggtact ttcttctgcc aaagccctgc caagcattgc cgatgcagta
      961 aaaggggaga tcaaaatcct tgccgactct ggtatccgca acggcttaga tgtggtgaga
     1021 atgctggctc tcggcgcaga ttgtacgatg atcgggcgtt cctttgttta cgctcttagc
     1081 gccgccggtc aagccggtgt ggaaaacctg ctcgacatct tccttaaaga aatgaaagtg
     1141 gcgatgacac ttaccagcaa tgctaaaatt agcgacatcg gcagagatgc tttggtagat
     1201 ttaagtaatc tttagctcaa atagccacaa tccgcacgtt ttaggtgcgg attgttattt
     1261 tagataggct aatttggcat tttggtaata ttaatgcggt aatatttcaa tgtatcttgg
     1321 ttgaaaaaga gaaattcatc gttatcttta gttttttcgc ccagcacata aatgttattg
     1381 cgatgaatag ataagacata atatcgctta ttttcaattt caagataatc atacgttgtt
     1441 ctcagctcag ggcgaatgta gccaatactc aaagaaagta agctaaaata gatgaaaata
     1501 aaaatataga aaaaaccgaa gcgttcttcg cttaacattt tttgaatatc taagtggaat
     1561 acgcggtggt cccaatgttt gtgaaataat aatactgata gggtagttgt ccctagatag
     1621 aggtagttgt ccctagatag agtaggtgta aatacattgg cactttgcca ataaacagat
     1681 agaaactgat cattactggc aagctaaaaa tagtaacaag aacactcacc cgtagccagc
     1741 caaggtattt aaagtaatgc agcttaaaaa ctttattaac taaagcgtat cctatcaaat
     1801 aaaaaagaaa gatagttaaa aatgtgccaa aaacgtaggc tagtgcgcga gctataccgg
     1861 cattaccaac ctcaacatgc caccacgggt agccatgaaa agcagcaata ccccaagcat
     1921 aacagtaagc ggtagaaaaa gagattaaag taaagaaagc gagagtaaga gaagagttga
     1981 tgaatttctg attagtcata acagtaaata gattgcacct acttagttaa agatgaaaca
     2041 caagaaagca gactgagttt agcttccacc caactccttt gtttagagga gaactttatt
     2101 aaagcacgac ttttgttgta tttcaaacct ttgtttttat acaaaacaac cgtgttgtaa
     2161 aaaatgatcg taaactgacc gcttgtaatg cactctgaat atatcgaaac atgataaaaa
     2221 tgataaagca tgtttcgata atttactgtc tttagtcttt taaatgtttt gcatgaaagg
     2281 ctatatgttc accgataaag ctactaatga aatagtagct gtgatcgtaa ccatcacgta
     2341 aattgaattg gtagggtatt ccaagtctat ctgcggtttc acagaaaatt tccggcttta
     2401 actgatcgct caaaaagctg tcttggctgc cttgatcgat tagaattggc aagcttgggc
     2461 gatatttttc cagcaatgcc acgctatcat acgccttcca agcggtctga tcttcgccta
     2521 aatatgctaa aaatgctttt tgcccccaag gaacttgggt tggggcgaca atcggtgcaa
     2581 atgccgatac actttgatat tgccccggat ttttaaatgc aatggttaag gcaccatgtc
     2641 cgcccattga atgcccaaaa attgattgtt taccgttagt cgggaaatga gcattaatca
     2701 atgttggcag ttcgctgaca atgtaatcat acatttggta atgtgttgtc cattcgcctt
     2761 gcgtagcatt taggtaaaat ccggcaccct gacctaaatc ataagcctca tcattagcga
     2821 cattcacacc cctagggctg gtatccggtg cgaccacaat cagatgatgt tcgtcggcat
     2881 aacgttgaaa gccagattta gtgatgaagt tctgctcggt acaggttaaa ccagaaagcc
     2941 aatatagtac gggtagcttt tgtgttttag cagattgcgg cagataaata gcaaatttca
     3001 tttcacagtt taagctggct gagaaatgtt gccaaacctg ttgttcaccg ctaaaacagc
     3061 ggtagttttc aaattgttgc ataatattcc ttacaagcgg tttaattccg ctgatttttt
     3121 gcaaaaataa aggcggaact caccgccttt atattgtaat atcaaccttt caaacttccc
     3181 atatcaatca caaaccgata tttcacgtct gatttcagca ttcgttcata agcatcattg
     3241 atgttttgca tatcaatcat ttccacatcc ggcacaatgt tgtgttcggc gcagaagtcg
     3301 agcatttctt gagtttcctg aatgccgccg atgacagagg cggaaataga gcggcggcct
     3361 aaaatcatcg gtacggtgtt gatggtttgc tccaactcac cgaccaagcc caccagcacc
     3421 aatgtgccgt ccaacgctaa ggttgggata taaggtttca agtcgtgggt ataaggcacg
     3481 gtatcgatga ttaaatcaaa ggtatttgca acgtctgcca tttgcttttc atcggtagaa
     3541 agcaccacac ggcttgcgcc caaacggtaa gcatcatcgg ctttacccag tgagcgagta
     3601 aaaagcgtaa cttccgctcc cattgctttg gcaagtttaa ccgccatatg ccccagtccg
     3661 ccaagcccca caacagcgac ttttgagcca gctttgacat tccaatgacg caggggcgaa
     3721 taggtggtaa tgcctgcaca caaaagaggg gcaaccgctt tggtgtccaa gttttcagag
     3781 acttttaata caaaatcttc actcaccacc acgcatgtgc tgtaaccgcc ttgtgttggt
     3841 gtgccgtcaa agcggtcaat accgccataa gtgccgatgt tgcccacttc gcagtactgc
     3901 tccaaaccgt gctggcaagg actgcattca cggcaggaat ccaccataca accgacaccg
     3961 accaaatcgc cgatttggaa ttttttggcg tttttaccga ctttgataat acgaccgatg
     4021 atttcgtgcc ctggtacaag cggatagctt tgcgtccagc cccaatcatt gcgagccata
     4081 tgcaagtctg aatggcacac gccacagtac aaaatatcaa tcaaaacatc gtcttcaagc
     4141 aattcacggc gttcaaaact gtgtggggca agtggtgtgg ttgccgaaaa agtggcgtag
     4201 gatttgatag ggtaagccat ttttggggtt taaatggctg aaatggcaat tactatagtg
     4261 ccgatgattt attattgaaa atcaagattt ttgataaatc ggtatcagtt aaaatgataa
     4321 ttgggggttt tcagactgac tgaaaaatta ttttgcccat tttaaaaaca aaaacaccgc
     4381 cctcatcaca aaagcggtgt ttttcatcaa aattgtgtca aagcatactc tgccaattct
     4441 gtaagcactt catcagcagg acgctgagtg ggttttaaat tgagtgccaa atgtttcacg
     4501 cctttttctt gttcttttgc ccaaaagtct gccaagcctt ttgcaccacc acgcagataa
     4561 atgttgttaa ataactgtgc tttggcgttt ttgtcgtcca aaacttccac aaattgagca
     4621 tagccaaatg ctgtgggggt gtctttttgg cgtaaatcgg caagggtgtt gatgatgttt
     4681 gcggtgtcgg caggattcac accgtgccaa atccacgcat caggtacatt agcaagccag
     4741 tccatttctt gccttgctcg tccaatcgcc acaatgggca agcggtgttt gggtttgggg
     4801 agcaaatcca aattgtctgt taaatcggca taatgtttgc ctttgaaatg gggaaaatcc
     4861 tgctcggtta aggtgttaat caaatcaatg ctttcacgaa aacgctcgct acgagaggca
     4921 aaatccgccc caaatgcagg gtattccaca ggtctatcgc cagaggataa gcccaataaa
     4981 aaccgtccat gactcaaact atccacactt gtcgccattt tggcaatgtg aatgggacta
     5041 cgcaaagggg caatcagccc tgccgtgcca agggcgatgt tttgggtgtg ggcggacaaa
     5101 aaaccaagtg taaccatcgg gtcaagccct tgccccacat cgccaaaatg agggtcataa
     5161 aacggcacat ctcgtaccca caatgctgaa aagcccaagt tttcagccag ccgtgcattg
     5221 tcggcaatgg tggtcaaatt gtctggaatg tgattttgat agcccataaa gggggcaatt
     5281 aagcccagtg ttaattgatt ttgcccaaaa acttgggaga aaatggggga aagcgtgttc
     5341 attttggcgt acctgtctta tttaagataa ggtattttaa gggcttttga gtttgcttaa
     5401 aatgggataa attgagaaag tattttgcta aattggcata aatagacgct ttcaagctga
     5461 ctgaaaattt gcaaaatttt taataaaaat gaccgcttgc ctttcaacaa gcggtcattc
     5521 ccctttaatt ttttaccacc gtaaacacct tatttttcag ccgatattca cttttttcat
     5581 cgtgagccag ttgcacaatg gttggcttgc gtttacacaa ttcacgcatt gcacacgccg
     5641 tcaatgttgc cagcaagcat atcttgttca gagtaagagg catcgccgcc aatcaaaata
     5701 taatgctcgc ccatatccac caccaccgcc aaatgcccca gcgtatgtcc tgcggttggc
     5761 acaaggcgga ttttgccgtc tttggtgagt ttatgactgc tctcaaaact ttcaaacgcc
     5821 ccatcattaa aatagatgcc atcaggctta aaccaatcag gatagtgcat tgttaaataa
     5881 ccgttcatca ccgcattggg ggcgttgatt gcctgttttt cagtctcact catcacaaat
     5941 cagctgtttg ggaaattggg caatccgcct gcgtggtcgc cgtgcatatg ggtcattacc
     6001 accgtgtcaa tgcgtaaagg gtcaaagccg tttgccttta aaatttcgcc cacttcttcg
     6061 tgttttttga ccccacgccg ttcgcaaaac tgcataaacg ggtgccacca cggctgatag
     6121 cccttgtcgt tggcacgact gctttcgccc gtatccacca aaatcaaacc gtcatcgtgt
     6181 tcaatcagcc agcaaccaat cggcagttta ggcgaccatt ttttatcggt cagcacatca
     6241 agcacacggg caggacgggc aaaaaagcgt gccacttgat gatgaatttt gatttgtacc
     6301 caacctgttg tcagacaatg cactttaatc atttttgtgt tctcattaaa aaatcgtggg
     6361 catattgtag aaagtgcggt aacttttcac aaacattaaa aagttcgtat ttggctggat
     6421 atgcagataa aattacaaaa atgtatgctt attttgaaaa aagatgaacg atttaagagt
     6481 aatcaaaacc catctagcaa tccaaaacgc tttgattgaa ttattagatg aaaagctgtt
     6541 tgaaaaagtg caagtccaag aaatcattga aaaagccttg attaaccgca ccacttttta
     6601 ccgttattat tcgggcgaaa gtgatttggt cggcaaattg ataaaagagg tgaaggcgga
     6661 atatcaagac ctgctcaaaa agcgttttga aagcggtaat ttgctgcgat ttatgcaaac
     6721 cattggcaac cgcttattgg aaaaaagacg gctgattttg gcactttggc aaatcaaaac
     6781 ccctcgccat catttgtatg acgatatgca catgctgtta aaaaacgcct ttatcgccca
     6841 cgccaaacgg ataacatcag atgataaaaa ctgggctgag aaaaattggg attaccaagc
     6901 ccatattttt gccaccattg cccttgaaag ccataaatat tattttgaac aaggcaaact
     6961 tttgcccatt ccacaggtgt ttgaggagtg gatagaaatg gtgcaggtgt tgaaaagtgt
     7021 ttagaaataa ctggtaaaat tttccataaa aaacaccgct ttcatcacaa aagcggtgct
     7081 ttcaaatcat caaaccttaa ccacaatctt accaatctgc tgattgcttg ccatatattc
     7141 gtgagcttta accatttcat caaaggcaaa gattttgtca atttcagggc gtaactgacc
     7201 gcttgccaaa ccgtcataga caaaggtttt ggcttgtgcc aatttttcag gtacggtggt
     7261 aatttcaaag agttcatacc cccgcacggt caaatgctta cccaaaatcg ggaacactgg
     7321 cacagcaata tcatcatggc ttaacgctcc ataaatgatg taacgaccat cttgtgccat
     7381 tgcattgatg atttttgccg cctcttttct gcccacaggg tcaaatacca cattgacgcc
     7441 tttgccattg gtgatttcaa aaagtttggc ggttacatcg tcttcgcttg tggcaatcac
     7501 aaaatccgct cctttttcca aaagtacatt gcctttggcg tgggtgcgag acaaggcaat
     7561 cacggttgcc ccttgcattt tggcgatttg gatactggct aaaccaaccg aactggttgc
     7621 accgcccagc accacaaaat cgcctttttg tactttgcca aactcaatca gtccgccata
     7681 agcggtaaca aacgccatcc aactggcgga cgcttgttcc attgacaaat tatcagggtg
     7741 cttgaccaca gcgtgttttg gcatattgac gatttcgcca taagtgccgt attcggtaaa
     7801 cataaaggac ggaataatgc tcaccttatc gctaatggca acttcgttca caccctcgcc
     7861 aatcgccaca atcacaccag ccccttcata acccaaagtc gcagggaata cagggtcaat
     7921 cacatacgcc ccttcacggt acatcatttc ggcacggttt aagccaatgg cttggatttg
     7981 gatttgtact tcgtgtgctt ttggtgcagg aattgctaca tcaacgattt gcaagacatc
     8041 aggcgaacct gtttttgtaa attggatttg tttgctcatt gttttgacct tttgtgtaaa
     8101 taatgggcgt attttaatgc aatgaattta ttttaaacac aacatcaatt gcaaaacact
     8161 tttgctaaat ttacataaat acattttcac aatgtctgaa aagttgtttt gcaaatttta
     8221 aaaagaaaac caccgcttgc cgatcacaag cggtggtttt tgtgggttat tttgcaagag
     8281 gtgtcagctt ggctacagta agcggataaa tgcgacgccg gctaagagta aggtaacgcc
     8341 gatgatctgc agcggtagaa tgggtttttg tgtaatgccg aacagaccga atttatctac
     8401 cagcaggctg ccgcaaatca ggccagagag gacgagcatc accaccgagc ctgtgccgat
     8461 ttgggggacg agccaaacgc cagcgaggat aaacaggctg cccagtatgc cgccgctcca
     8521 tatccaaaac ggtttgcctt cggttggctg gcagagtttc ggcaggcttt tttcataaaa
     8581 cagcactacc agtaataagc cgatggcacc gacaccgaag gagactgttg ctgcgccgag
     8641 tgatgagccg ggctgtctgc cgaattcgcc attaacggcg gcttgggttg ctaatgcggt
     8701 tccgcctaat atgccgactg cgcgccacag ccataaattt gcgttttctt tgcgttttag
     8761 accggacaga tggggcagta ccacggcaat ccatacgccg aataagacta aaccaatgcc
     8821 aaacaggcgg ctggccgtga aggctatgta aggcgagtta agccaagcga aagtgtcgat
     8881 cagcacgccc atcagcactt ggcctaaaat cggcatgacc gcagtttgta cgccgccgag
     8941 cttggggaag atcagtacgt ttgccgttag ccccaccatg ccgagtaatc cgcccgtcca
     9001 tgcccataag gggacgttgc ctaaattgtc gaggctaaat gctggtgttt gttgcatgat
     9061 gagcgagagt ccgagtagaa acaggaggcc tacgctgaaa gagacaaacg aggttaaaaa
     9121 tgccgcaccc acgtgcatac gcaaacgggc gttgatggct gattgaaatg caatcagcac
     9181 gccggctaaa ataccgataa taaatgtcat gactaactgg gttgtgctgc cgcctcaaaa
     9241 tgagtggtgg ttgaaaagaa atgccgtctg aatgcggatt cagacggcct taaggcttgg
     9301 tatcggcgtt tacctgatgt tgaatagccg ttttgcctag ccggtttatt cgatgaattt
     9361 caccacggtt tcgaaatctt tacgtgcttt ttggcggatt tctttatcac ggtaaccgaa
     9421 ggtacacatc accgatacgc cccattcgct tgggtcaaat aatccttccg ctgccaaaat
     9481 atcgttgact tcttggtaag cgaaaccttc tatcggacaa gaatctacgc caattaaagc
     9541 tgcaccggtc atcatattgc ccaatgcgat gtaggtttgt ttgcttgccc agtcaaacag
     9601 gctgcgttcg ctgtctaaaa tgccgatatc tttctcttgg aattttttat acacggccat
     9661 ggctttttcg cgcgcttcgc cttgtaaacc tcgtctatcc ataatttcag aaaaatattg
     9721 gctgtcgtaa cgggcatttt tcttcgctaa aatcaccacg atatggcttg aggtttccat
     9781 agttggaatg ccccagcagt aaggtttgat tttttgccgt aattcagggt tttgcaacac
     9841 taaaaactgc caaggctctg agcctaccga acttggactt aaacgaccga gttccagaat
     9901 atagttgaaa tcttcagccg gaatttttct gctgccgtcg taagaacggg tagatgcacg
     9961 gtaatgaaag gcattgatta cgtcttcttt gttgaaaaat tgcatggaac actcctaaaa
    10021 atgcaaaaca gtcttttcag acggtctgaa aagtctagat gaaatacgga taagtataaa
    10081 agatttaagt tatttttaat aattgataat tttgatattt aggtataagt tgacgtgatg
    10141 agaaaaaaca gaccctctga atgttcaggc ggtctttaac gttggttgtt tacataaata
    10201 atccgcgttc atattgcttc ggtacaaact gcatttcact gctgtctgct gcattttggt
    10261 catattgcgc attcaatacc cagtacggat cgcgcaataa ggctcttccc acagccacta
    10321 aatccgcatc gcccatacct aagatatgat cgcctaccgc cggattatcc aacataccta
    10381 ctgcaattac cggtttgccc gtagcgagct tcacttgacg ggcaaagtct acttggtaac
    10441 ctgcgtaaaa ttcggggtga ttacctgcat gcaatacacc atcaccacca ccgctgatgt
    10501 ctaagacatc tgcgcctgca tcggcaaaac gtttcgctac ctgcaaacca taatccaagc
    10561 caaagccgtt ttcaccgtat tcttgtgcag aaatgcggac aattaacggc atttcagacg
    10621 gcatcacttc acgtgctgcc tgaatcactt gctcgccaaa caacattttg tcttggccgt
    10681 actcatcact gcgctgattg gatttcggcg aatagaattg gtgaattaga taaccgtgtg
    10741 cgccgtggat ttcaattgta tcaaagcctg cctcaacggc acgtttgacc gaatccttaa
    10801 aggcttgaac caatgctttg acttcgtctg tcgtcagctc gcgaggtgta atcagatttt
    10861 gtccggcata atccaactca ccgtagtgaa ttgcacttgg agccactgca tccggctcgt
    10921 cttgggcttt gcgtccggca tgggcgattt gcacaccgat ttttgcccct tgagcatgaa
    10981 cgctgtctac cagttttttg aatgcctgtt gttgttcatc gttccataag cctaagcagt
    11041 ttggcgtaat tctgccgttt ggcgccacat tggtcatctc cacaataatc aagccgacac
    11101 cacctactgc acggctggtg taatgcacga aatgccaatc gttcggtacg ccgttggttg
    11161 ccgaatattg acacattggt ggcataacca cacggttttt caatgctaag tttttaatgt
    11221 taaacggggt gaataatttt ctgaacttcg ccatgttgcc ttccttaatg aaatagatta
    11281 ggtggaatga taattcagtg ctattatcta ttcaaattga ttattgtgat agttaggtat
    11341 aaaaatgagt gatataagaa cactggattt caatttgctc aaagcttttg tggtgctgct
    11401 ggacgaatgc aacgttagcc gggcagcaca acgattatcc atcacccaac cggcaatgag
    11461 cggtatttta aaccgcttgc gcgagagttt taacgatccg ttatttgtgc gagtgcaaca
    11521 cggtatgcag ccaaccgacc gcgcgttgca actggggcaa accgcacgca acattctgca
    11581 agatatcagc tccatgctgc aaccacccat attggaaccg gaacatctga atatgacctt
    11641 gcgtattgcc gcaatggatt atgtacaaca aattattgcc ttgccgctga ttctgcgttt
    11701 aagacggctt gctccgaatg taaaagtcgc cttactgccg gtgcaggggc aaaatatcaa
    11761 aactttgttc gagcaaaaaa aaatcgatct tgccttggtc agccgccaac atttaagcac
    11821 ggatacgctg aaaacggtat tatatgaaga acgctacgta tgcgcgatga gcaaaacaca
    11881 cccgatggcg caaacaccgc tatcgctcga tcaattctgc aatcttccct tcgcaatgct
    11941 ttcttacaac ggcggagaat tcagcggagc tacggatcta gcgttacaaa agataggact
    12001 acagcgcaaa gtaatggtct cggtaaacca tatttccctg ctgccccagc tgctacaagg
    12061 ttccgactta gtcgcagtat tgcccgaacg cttagcaaaa accttaccca atgtttattt
    12121 acaacctccg cctatagagg tagaaggctt caccatgatg atggcttggc acgaaagaac
    12181 cgaacaagat acggcgcatc gatggttaag agaggtgctg cagggtgtgg taaaacctaa
    12241 acaggctctt taattattct ttcttttgct atgaaaaaag ccgtttatat ttttagataa
    12301 acggcttttt tgcattctaa tattagtatt tttctacaaa ctgagcatag gcattaatga
    12361 agttttgcat tgagcttttt aatgtctcag aggcaatttc accctgttca tcaaatacgc
    12421 ctgtaaataa gtttcctacg tttaatgcga aaggtaaggt tggcatattt acaaatgagc
    12481 ctgaagtaat agtacgtact tgatcagctg cacgtacacc gcctgccggg cttgcgccaa
    12541 cggttacgat acctgccggt ttgccaatcc atttgctttg ccccattggg cgagaaacca
    12601 cgtcaatcgc atttttaagc attgcagaaa agcttgcatt atgctcaggt gtgactaata
    12661 acacgccatc tgccacagca accgcttcac gcactctggt gtattgtgcc gggctgtttt
    12721 catctaaatc acggtcgtaa agtggtaagt ctgcaatatc cacaatgttt aattgaatgc
    12781 ttgcaggtgc cattttttgt aagtgacgaa cagtcaagtg actaaatgaa gttttgctgc
    12841 cgctaccaat taatactgca atttgttttg ccattttata attccttagt acatagagaa
    12901 tataggcatt ttatcccttt caatctgttt cataaagcgc ttatgatgaa aagcactatt
    12961 tacttatatt aaataatttt gcaaatttta ggccaaattt gaccgctttt atctttagct
    13021 aaaaaggtta taatttgtag aaatagatct taatttaggg tttttatgct ttttctaaac
    13081 aaaataaaac agcttttcat cttcgccttt acgctagtgt tatctgcttg cagtttggtt
    13141 gtttataagc cagttgaaac aattaaacag gtaaatgttg aaagaggtta tcgacaaaaa
    13201 aatgtgatcg ctgaaagtgc aaaagacggt aatattgttg tcatcatgtt ttcaggtggt
    13261 ggaacaagag cggcggcgct tggttatgga gtgttggaag agtttaaaaa ggtaagggtg
    13321 aaagaaacaa ataaaggtaa tactttatta gagaatatag atattgcctt tggcgtatca
    13381 ggtggatcga tattagccac ttattttgcg ttggaaggaa aggatatcat tcctaaattt
    13441 gctgaccgtt ttttaaaagc gaactttcaa aaagatatta ttacagaggt gttttcgtta
    13501 tccaatcttc ctcgtttaag ttctcctcaa tatgggcgtg gtgatttatt gcaagaacgc
    13561 ttaaattttg ccctttatca tggtaaaaag tttggtgatt tagtgaaaaa tcgtaaagga
    13621 ccttttgctg tgattagcgc aacagacatg aacattggtc aaaaactgac ctttacgcaa
    13681 gaaacctttg atgggctttg tttaaatttg aatgatttag aaatttcccg tgctgttgct
    13741 gcttctagtt ctgttcctct gatttttgcg ccgcttacac tgaataacaa tggtggaaat
    13801 tgccatttta gtatgccaca agaatttagt gctagagaaa aagcaattga tggactaatt
    13861 acaaaaaata ttgaagaaac gaatcatata ctcagccttt atcaagatag taaagaacgc
    13921 ccctttatcc atttagttga tggtggatta acagataatt tagggcttgc cagtgtgctg
    13981 gaaatatctg atgttatggg tattgaagag atgtaccagt atattcatca gcttagggtg
    14041 aaaaatattg tagtgattaa tgtaaatgcg caaaatgaag tatctagcga gatcgataag
    14101 accgcaaatg taccgggggt ggctgatgta gtgaatacaa tagttaatgt gccaattgat
    14161 agaacgacac aaattaattt acaacgttat cgccagttta cagatggttg gaatgaatat
    14221 gcaaataaac agccatcaaa gatcgaaatg cattttgtca gtttaggttt aaaagaatta
    14281 cctgatggtg atttaaaaaa agatgtgcta aatattgcaa catcatttta tttgccggaa
    14341 gaggatgtag ataaattacg tgaagctgct cgggttttat tgcagcaatc tcaagaatat
    14401 aatgcggcat taaaaacact gcagtagtaa aaaaccgacc aagcggtcgg tttttggtat
    14461 ttttttgcaa atatttattc gctgatgtcg tttaaatcca acaactcttg cgaaagaata
    14521 atgccggtta agtcagcata aacgtaatct tcggggaaga aggttacacc gccaaaattc
    14581 acaggcgtat ccacttcgcc aatgtcggta ttatctgctc ccactggaat tggcgctaaa
    14641 gcatgaatac cgatatctaa tctttccaaa atatctaatt ggcgaaccgc accataaacg
    14701 atgatacctt cccagccgtt atctacagca agttgagcca gctctgcgtc aattaaggca
    14761 cgacgaaccg caccgccacc atcgactaac agcactcgcc cttcaccctc ttcttccaaa
    14821 acttcagcaa ttaaaccgtt gctttcaaag catttaaccg tggtaatctt cccaaagaag
    14881 gaagaagcac cgccaaagct tgagaaaatt ggttcaacca catcaacttg gtcagaataa
    14941 agatcgcaaa gtgcagaggt atcaatacgc ataatatgct cctgttcgta gattatttag
    15001 ctaaagtata aaccttcgcc caaaatttgc aaaattttat cgaaaaaaga ccgcttacag
    15061 gcttttttct tatgctttat tttctagtga accgctttta atatgcaatt ccatttggtt
    15121 gtaaggaata gatatacctg cttcaccaaa ggctaatttt acttgttcga taatttccca
    15181 atatgctgcc cagtaatttg gtgtctgaac ccaagggcga acaacaagtt gaacactatg
    15241 cggtgctaaa gcgccgactg ctactacagg tgccggatct tttaatacat aagtattggc
    15301 gtctaaaatg cgttggataa tctctttcgc ctcttttaag ttagattcat aagaaatatc
    15361 aaaggtgaaa tcaatacggc gtgtcgggtt gttagagtag ttgatgatgc tgtcagaaaa
    15421 cactttgccg tttggtaata acacggtttt gttatcgccg gtgcgaagtt ccaacactaa
    15481 aagccccatt tgttcaacca cgccggtcat tccgccggtt tcgattaaat cccctttgcg
    15541 aaatggcttg aagattaaca acatcacgcc ggcagcaaag ttttgtaatg agttttgtaa
    15601 tgataaaccg atggctaaac cggcagcacc gattaatgcg accagcgagc tggtgttaat
    15661 gccgatttgt gaaagcgagg caatcaccac aattaacagc agtaaaaaat agctgattga
    15721 gacaataaaa ctttgcagca tttcatcttt ggttgatttt aatgcggctt taccgagtaa
    15781 acggctaatt aggcgggcaa gaaatttccc gactacaaaa atcgcaatcg ccagtaaaat
    15841 tttggtgcca taagggataa tccactcatt tagcatagtt tcaatattca tattactgac
    15901 tttgtggatt actttttcgg tttcggcaac cacgtcaatg tttggagctg tttcggtgac
    15961 tttttcagtc atattgtttc cttaaaatta aaatgaaaaa taagcctagc gaatttgcct
    16021 cactcgctaa gcggttttgt gctagaattt agagcaaaat tttctctaaa agacaagata
    16081 atgacaacag aaaaagtaat tagtgccggc gttcaatttt ttacgatcag tgaagatgaa
    16141 gccggacagc gtttagataa ttttttattg gcaaagctca aaggcgtgcc gaaaagtttg
    16201 atttaccgca ttgtgcgaaa aggcgaggtg cgggtgaata aaggacgaaa caaacccgaa
    16261 tataaattac agcctgatga tttagtgcgt gtgccgccgg tgcgtgtggc ggaaaaaaat
    16321 gaagccccaa tttcgactaa attaaacaaa gtggcggagc tggagagcca aattctgtat
    16381 gaagatgagt caatgctagt aattaacaaa ccttcaggca ttgcagtaca cggtggcagt
    16441 ggtttaagtt ttggtgtgat cgaagccttg cgtgcattac gtccacaggc tcgtttcttg
    16501 gaattggtac accgtataga ccgtgataca tcaggcattt tgctggtggc gaaaaagcgt
    16561 tcggcgttac gttccttgca cgaacaactg cgtgaaaaag tagtacaaaa agactatctt
    16621 gcccttgtgc gagggcaatg gcaggctcat accaaagtga ttaaagctcc attgctaaaa
    16681 aacgaacttg ccagcggtga gcgaattgta cgggtgagtg aagagggtaa accgtccgaa
    16741 acccgtttca gcattgaaga gcgttatgcc aatgccactt tggtgaaagc ttcgcctatt
    16801 accggaagaa cccaccaaat ccgtgtgcat acccaatatg ccggacaccc gattgcgtta
    16861 gatgacaaat atggtgatgc ggaatttgac agcaaaatga aagaagtggg cttacagcgt
    16921 ttatttctgc acgcccatgc tattcgtttt gaacacccca aaaccggcga agaaatggtc
    16981 attaccgccc cgctggataa aactttaaaa ggcgtattgg caaaattaag ggctaattac
    17041 taaataactt aatgagagag taagatagcg caagcgtcca cgcttgtgct gtaaatcaac
    17101 atagagtggt acaagcgtgg acgcttgcac caacaaaata taagcaaaaa tatgcaaatc
    17161 catttcaaaa attttcaaaa aaatgaccgc ttgttagatt taaccacacc gcaaattatg
    17221 gggatcttaa acttcacgcc ggattcattt tccgattcgg gcaagttttt ttccctcgat
    17281 aaagccttgt tccaagcgga aaaaatgtta gctgatgggg caagcattat tgatattggc
    17341 ggtgaatcca cccgccctaa tgcggcgatt gtgagcttag aacaagaatt agagcgtgtt
    17401 gtgccggtgg tggaggcgat ccgcagccgt tttgattgct tgatttcggt tgatagctcc
    17461 aaagcagaag tattcaaaga gtcggcaaaa gtcggtatgg atatcattaa cgatattcga
    17521 gccttaaccg agccgaatgc gttggcagcg gcagtagaat tgggcttgcc ggtttgttta
    17581 atgcatatgc aaggcaaccc gcaaacaatg caacaaaatc cgcactatga tgatgtgttg
    17641 gaagatgtgg cagatttctt aaatcaacgg attttcgagt gcatcaccgc aggcattccc
    17701 aaagaacaca ttattttaga ccccggtttt ggctttggta aaagcgtgaa acacaattac
    17761 caattattga aacaccttaa agcctttgtg gattcaggct tccctgtgct tgccggactt
    17821 tcccgtaaat caatgattgg tgcggtgtta gataaaccgg tagaacaacg gctacttggc
    17881 tctgtagtag gggcgttatt agccgctcaa aatggggcga aaattttacg ggtgcacgat
    17941 gtggcagaaa ccgccgatgc gttaaaaatc tggcaggcaa tgctggaatc agataatgag
    18001 gaaaaatgat gacaacctta gtttatggca ttaaaaattg cgacacagta aaaaaagcct
    18061 tgaaatggct cgaagatcat aatcaaaacc cacaactgca cgattaccgt gtggacggtt
    18121 taaatgccga ttggttagtg gaaatggaag caaaattcgg ttgggaaaac ttagttaata
    18181 aacgtagcac cacttggcga aaccttgatg aggaaattaa aaacaacctt aaccgtgagt
    18241 cggcattaaa agtgctaacc gaacagccga ccctaattaa acgcccgatt gtgattaaag
    18301 aggatattgc tttgattggt tttaatgaga aagagtatgt ggctgcgttt aattaatttt
    18361 ttcccctgct tgtagggaca ggtcctgtgc ctgtccgtag tctttaggat tgttcgggcg
    18421 ggcacaggac ccgcccctac aggatatata gcaaaaaatt cacccaaaac aaccgcttgt
    18481 aacctatgaa aaatgaaatt atttcccttg cacagaacct aatccgccgt gattccatca
    18541 gcccacaaga caaaggctgt cagcaagaaa ttgcacaacg tcttgaaaaa ctgggcttta
    18601 atattgaatg gttgccgttt ggcgacacct tgaatttatg ggcaacgcac ggctcggaaa
    18661 atggtaaaac agagccttgc attgtgtttg ccgggcatac agatgtggtg ccggtaggcg
    18721 atgaaagcca atgggcttat ccgccgtttt cggctgagat tgtagatgga atgctttatg
    18781 ggcgaggggc ggcagatatg aaaggctcgc tctcggcgtt agttgtggcg gcagaaactt
    18841 tcgtgcaaca tcacccaaac cataagggca aagtggcatt attgattacc tccgatgaag
    18901 aggcagcagc caaagacggt acggtaaaag tggttgaaac cttaatggct cgcaatgaag
    18961 cggtacatta tgcggtagtg ggcgaaccgt ctagtagcaa ggtgtttggc gatgtgatta
    19021 aaaacggcag acgtggttca attacggcag atctctatat cgagggcatt caagggcacg
    19081 ttgcctatcc acatttggcg gaaaatccgg tgcatacgtc actgggtttt ttaaccgagc
    19141 tgaccactta tcagtgggat aacggtaacg aatttttccc gcctaccagt ttgcaaattg
    19201 ccaatatcaa agccggcaca ggcagtaata atgtgattcc gggcgagctt tatgtgcagt
    19261 ttaacttacg ttattgcact gaagtgaccg atgaaatcat caaagccaaa gtagcggaaa
    19321 tgctcgaaaa acacggtttg aaataccgaa taagttggaa tttatcaggc aaaccattcc
    19381 ttgccggcaa tggaaaattg gtcgaagccg ccttacaagc ggtcgaaaat gtcacaaaaa
    19441 ttacgcctcg tttagacaca ggtggcggta cttcggacgg tcgttttatt gcattaatgg
    19501 gggcagaggt agtggaattt ggaccactga acgccactat tcacaaagtg aatgagtgtg
    19561 tgagtgcgga tgatttggca cattgtggcg aaatttatta tcaaatttta gaaaaattat
    19621 tggcaactgc ataaccctaa ataggcagaa tgttatcaaa ttgtattctg cctatttttt
    19681 atgtagctat tctgttaaac agcgtttaca ctagcggcag tttttatatt tataggtaaa
    19741 tgttatgcaa ctctactctt atatctgctt gctgtttggg cttgcgatgt ttatttcgtt
    19801 ttttacccga aaattaaacg aaaatattca aaccactatt gcaattacag cctctgccct
    19861 tgtcggctcc cttcttattc ttaccttcgg ttctctcggc tggtttaatg ttgataaact
    19921 tgccatttcc atttttgaac aactcgattt taaaagcttc ctgttaaacg gaatgctggg
    19981 gttcttgctt tttgccggtt cacttgggat taaactacca ttaatgaaag agcaacggcg
    20041 ggaaattgca gtttatgcct tattttcaac cttagtttct actttcttga tcggtgcgtt
    20101 aatttatggc gtggcaaggc tacttgggtt agagctcggc tttgtgtatt gtttgctgtt
    20161 tggctcactg atttcgccaa ccgacccgat tgccgtattg gcaattatca aaagcctgaa
    20221 agccccaaaa cgcctttcga tgcacgtgga gggcgaatct ctgtttaatg acggtatcgg
    20281 cttggtgatt tttaccacta tctttgccgt tgcttttggc ggacaagaac caaaccttag
    20341 cggtattacc ggactgttct tgcaagaagc ggtaggcggt atcgtttttg gcttattaac
    20401 cggttttgtg gcacataaat ttatttccgc aaccgatgac ggaagccttg aaattttaat
    20461 tacgctaacg attccaacag tcggctttgt gattgcaaac tacctacacg tttcaggggc
    20521 attaacaatg gtagttgccg gtattatgat tggcaactgg actcgccgtt ccggtttctc
    20581 cgaacagagc caaaaatatc ttgatcactt ctgggagatg atcgaccatt cattaaacta
    20641 cctgttattc ctgcttatcg gcttaagtac actgttagtt gatttcacct ttattggtgg
    20701 ggtgttaatg ttggcagcaa ttccaatttg tttgcttgca cgctatatca gcctgtggat
    20761 tccataccaa gtgttaaaac gtttccgtac ttataacccg tacaccttaa aaattatgac
    20821 ttggggcggt ttacgtggtg gattagccct tgcgatggca ctttccgtac cggcaggggc
    20881 tgcggtggta tcgagctcag aaggcaatct tgatgtacgt gatttgattt tgttaatgac
    20941 ttatgcggta gtcactttct cgattttagt acaaggttca acggttgaac cgatgataaa
    21001 aaaatccaag ttggtagatc caagaaaagt ggcactcaat aaattaggtg cacctgatga
    21061 tgtaattcca catatttaat agcatttata actgaataac atatcatttt gctaaagatt
    21121 attagccatc tagacggctt tatatttaaa tacctctcac gttaatttgt tgagaggtat
    21181 tttttatgtt attcacagga ttattatgtg gctttctact cgggtttgtg atgcaaagag
    21241 ggcgtttttg tattaccgga gcatttcgtg atctctatgt aacaaagaat agccgtatgt
    21301 ttgtggcact tttaattgca attacggtgc aatcgatcgg gatttggctt ttgtatgaag
    21361 ccggctcttt tagttctccg gcggaagatt tgcctttgct cgcagtgatc attggggcat
    21421 ttctgtttgg tatcggcatt atttatgcag gcggttgtgc tacaggaacg tggtatcgtg
    21481 ccggtgaggg tttaatcggc agttgggttg cactggttat ttatgggctg ttttctgctt
    21541 ctatgcgaac cggtgtgtta gccccactta atcaagagct aaaaagcaat gtaattcaac
    21601 accgcacgat ttatgaaaca tttggtattt ccccttgggt gttagtgttt atcctgagtg
    21661 taattacatt ggcattatcc gcccacagca tttaaaaggt taatttttta aggagtaata
    21721 aagatgaacg tacgtttacc tgcctcaggg cttgtttgcc cgtttccatt agtcgaagcc
    21781 aaagcggcga tggcaaaatt gaataaaggc gatagcttaa cgattgaatt tgactgtact
    21841 caagcaaccg aagccattcc aaattgggcg gaagaggaag gctatgaagt taccgattac
    21901 caacaacttg gtgatgcctt gtgggaaatt acggtggtta agtaattagc aataaaaaag
    21961 ctctactttt ttgtagagct tttgctttga gttttgtatt tactaaggat tagagtaaac
    22021 ccggttgacc taatgctggg tctgttgctc ttgcagcaat agcatcttca accgtcatac
    22081 ctaatgcttt tgccacacct tcaccatatg ccggatcaca ggcatagcag ttacgaatat
    22141 gacggtattt aatgaagtct aatgcatcgc ccatacctgc agcagtatta ttgaataatg
    22201 cttgtttttg ttcatcattc atcatattga acaatacacg tggttggctg aagtagtcag
    22261 catcgtcttc acgatagttc cagtgggctg catcaccatt aattttcagt ggtggctctg
    22321 catattgagc ttgttcttgc catttgccga agctgtttgg ttcgtagtga atggtactac
    22381 cgtagttacc gtccacacga ccttgaccgt cacgagcgtt gctatgaacc gggcaacgtg
    22441 gagcgtttac agggatttgg tggtggttca cacctaaacg gtagcgttga gcatccgcat
    22501 agttgaataa acgagcttgt aacatacggt ctggagacac gctgatgccc ggcactaagt
    22561 tacttggtgc gaatgcagat tgttccacat cagcaaagaa gttttcaggg ttacgattta
    22621 attcaaactc acccacttca attaacggat agtctgattt cggccatacc ttagttaaat
    22681 cgaacgggtg gtatggtact ttgtctgcat cagtttcagg cataatttga acaaacattt
    22741 tccatttcgg gaaatcgcca cgctcaatcg cttcgtataa atcacgttgg tgactttcac
    22801 gatcgttagc gataatagcg gctgcttctt cgttagttaa gtttttaatg ccttgttgtg
    22861 tatggaaatg gaatttcacc cagaaacgct cattagctgc attgatgaag ctataagtat
    22921 gtgagccata accgtgcata tgacgatatg aagccggaat accacgttca ctcattacaa
    22981 tagtcacttg gtgaaatgct tctggtaata atgtccagaa atcccagttg ttagttgcag
    23041 aacgcatatt cgtgcgtggg tcacgtttaa ccgctttgtt taaatccggg aatttacgtg
    23101 ggtcacgtaa gaagaacaca ggggtgttgt tacccaccat atcccagtta ccttcttcgg
    23161 tatagaattt taatgcgaaa ccacgaatat cacgctcagc atctgccgca ccacgttcac
    23221 ctgcaacggt ggtgaaacga gcaaacattt cagttttttt accgacttgg ctaaaaattg
    23281 ccgcacgagt gtatttagtg atgtcgttag ttacagtgaa agtaccgaac gcaccggaac
    23341 ctttagcgtg catacgacgc tcaggaatga cttcacgcac gaagttcgct aatttttcat
    23401 ttaaccataa atcttgcgct aataatggac cacgtggacc cgctgttaag ctgttttggt
    23461 tatcaacaac aggtgcaccg ttgttcattg tcaaatgtgt aacaggacat ttagacataa
    23521 tagaatcctc ataaattaga aata
//
LOCUS       DANLZC010000021        23305 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.021, whole genome shotgun sequence.
ACCESSION   DANLZC010000021 DANLZC010000000
VERSION     DANLZC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23305
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.021"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            33..1541
                     /locus_tag="PXE44_000660"
     CDS             33..1541
                     /locus_tag="PXE44_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL5588201.1"
                     /translation="MSNQSSNAPQRETFSGRGAFIMAAIGSAVGLGNIWRFPYTTYEN
                     GGGAFILPYIIALLTAGLPLLFLDYSIGHKYRGGAPLAFRRFSSKFETFGWWQVLINV
                     IIGIYYAVVLGWAASYTYFSFSGAWGDKPVDFFIGEFLKMGDITQGVSFEFVGMVTGP
                     LIAVWLIAIAVLAMGIKKGISAASNLLMPQLIVMFLVLVGYSLTLPGAAKGLDALFTP
                     NWEKLADPSVWIAAYGQIFFSLSICFGIMITYASYLKKETDLTGSGLVVGFANSGFEL
                     LAGIGVFAALGFMATAAGQEVNEVAKGGIGLAFFAFPTIINQAPMGALVGVLFFGSLT
                     FAALTSFISVIEVIISAVQDKLNSGRVKATLLVGIPMMVVSVLLFGTTTGLPMLDVMD
                     KFVNQFGIVAVAFVSLIAIVTSEKLSTLEKHLNEVSSFKVGFIWRLCIVITSGVLAFM
                     LFSEIAKVYREGYEGYPSWFVNTFGWGMAIGLVVVAYLLSKMAWKNEPKGDE"
     gene            1541..1639
                     /locus_tag="PXE44_000661"
     CDS             1541..1639
                     /locus_tag="PXE44_000661"
                     /inference="COORDINATES: protein motif:HMM:NF028261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HDL5588202.1"
                     /translation="MSSAAIITMVVAMTIIWGGLLFAIKRLPKEEQ"
     gene            complement(1687..1950)
                     /locus_tag="PXE44_000662"
     CDS             complement(1687..1950)
                     /locus_tag="PXE44_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HDL5588203.1"
                     /translation="MFVEIYGRLSCPYCVRAKTLAEKMKQELSDFDFKFIDMIAENIS
                     KQDLEPRVGKPVATVPQIFVDNNHVGGCTDFQAFVEEKFGITV"
     gene            2110..3444
                     /gene="srmB"
                     /locus_tag="PXE44_000663"
     CDS             2110..3444
                     /gene="srmB"
                     /locus_tag="PXE44_000663"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDL5588204.1"
                     /translation="MTQLMTFEELDLSPQLLKALAHKGYNRPTAIQQATIPAALEGRD
                     LLGSAPTGTGKTAAFLLPAIQHLLDYPRRKPGAPRILILTPTRELAMQVAEQAQALAK
                     FTQLSIATITGGVAYQNHGEVFNKNQDIVVATPGRLMQYIKEENFDCRAVEVLIFDEA
                     DRMLQMGFGQDAEKIAAETRWRKHTWLFSATLEGELLEDFAERLLNDPIKVDAEPSRR
                     ERKKIQQWYYHADNVEHKTKLLARIISEFSVERAIVFVRRREDVRELSETLRKRGIRS
                     TYLEGEMAQTQRNQAINRLKEGVVNVLVATDVAARGIDIDDIDFVLNYDLPYSADTYL
                     HRIGRTARAGKKGSAISLVEAHDYKLLGKIKRYTEEVLKPRIIEGLEPRTKAPKDGEL
                     NVTSKKEKARIKKKKEAKKEAAKPKVKVRHKDTKNIGKRRKPSSESAAVKQA"
     gene            complement(3513..3806)
                     /locus_tag="PXE44_000664"
     CDS             complement(3513..3806)
                     /locus_tag="PXE44_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5588205.1"
                     /translation="MSKTMQQKAAEAHNMCRLKGDSMLDNADRQIAFEAVYESEELAQ
                     QAVEFFAQKAKSVETEPCEIQSEITQIAEGFLMKMWITFSCQAEVILFQMAVR"
     gene            complement(3808..4470)
                     /locus_tag="PXE44_000665"
     CDS             complement(3808..4470)
                     /locus_tag="PXE44_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL5588206.1"
                     /translation="MPFPDEISELKGYFDGTNPVSAYPAQGLNVPFYILGSSPESAYL
                     AAERGLPYAFASHFAPRFMDDAIRIYRSHFKPSDVLDKPYVILGVNAIVAETDEEATQ
                     LATTQTQFFLNVVTNAQQNLQPPLESDDKVWENFVKAEIVPHFGPVAFEQNALYRQEK
                     TVVRQMTECSLIGSPQSVEQQLKALKARVEIDEIMAVNYIFDEQKQHQSYTWLKQITD
                     KV"
     gene            complement(4454..4906)
                     /locus_tag="PXE44_000666"
     CDS             complement(4454..4906)
                     /locus_tag="PXE44_000666"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HDL5588207.1"
                     /translation="MVLQVLNSKAIQPFLYANSENKMTNLSILNLVPIREGQTAKQAV
                     DSMVKLAKHAENIGIERYWIAEHHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLP
                     NHSPYIVAEQYGTLETLYPNRVELGLGRAPGTDMRTASALRPEFAFPR"
     gene            complement(4926..5564)
                     /locus_tag="PXE44_000667"
     CDS             complement(4926..5564)
                     /locus_tag="PXE44_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL5588208.1"
                     /translation="MKKFALKTVLVALSALFAFNATAVAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELNYKIPSQIKAKLPADSKLVQYHVNFLGRQSENLTRAWAL
                     AMALGAEDKVKTALFEAAQKDAFKSMDDIRAVFIANSISAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFSDSSSTNDFIQRYIDAVVFLSKK"
     gene            complement(5608..5877)
                     /locus_tag="PXE44_000668"
     CDS             complement(5608..5877)
                     /locus_tag="PXE44_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDL5588209.1"
                     /translation="MKCKRLEELLELLGEHWRKNPDLHLIDILQQLSVEVSEPDNFHA
                     LRDEVLIYQLKMRNADKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            5969..7078
                     /gene="mobA"
                     /locus_tag="PXE44_000669"
     CDS             5969..7078
                     /gene="mobA"
                     /locus_tag="PXE44_000669"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL5588210.1"
                     /translation="MEEKISAVILAGGLARRMNGAEKGLQLLDQKPLISHLLARLSPQ
                     IEDIWLNINRSVEQYQQLYPELPYYQDSLPDFQGPLSGMLSGFEQIESDYLLFVPCDT
                     PFIPTQLLHKLHTALRINQAQIAYAHDGDRPHPTVALVHRSVAENLRAYLASGERRLL
                     WFFQTQKSVAVDFSAYKAAFRNFNCLEDLTAFNPYPIKTLAITGYSGTGKTTLLEKLI
                     PKLTACGIRVGLIKHSHHNVDIDKKGKDSYRLREAGANPTMVVCDERWALMTETKQAA
                     ETKQAVEFSQLIAKFDPETVDLVLVEGFKHETMPKIQLHRKEIEKPLPALDQWTIATA
                     TDYPLERENRLDINDVEQLADFIRQWLEASKEPAN"
     gene            complement(7133..11644)
                     /locus_tag="PXE44_000670"
     CDS             complement(7133..11644)
                     /locus_tag="PXE44_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5588211.1"
                     /translation="MINKNFPLSYLASFVAFAVSLSATASVFRDDIDLQYYRDFAENK
                     GKFTVGASNIEIKDKSGNTLDALFPNVPMPDFSAANRNLGIATLVAPQYLVSVAHNTQ
                     YNTVEFGAPGTNADAHHYTYKVVDRNDYGIVEGGQHQDYQVPRLNKLVTEVAPATVTD
                     LGNNASAYQDSSRFTHFARLGSGRQIVKNTEHKDNQISTSYQYLTGGVHLPIAVHNSD
                     YWLDFRGNALNNSPYGALTAFGTRGDSGSGVYGYDQKTKRWLLLATYTFGTPANNYYN
                     RAGIIRQDYHDKQFAEDIAGTLTNAQQNAVFEWSAQGKDSSIGNKGKNLTVSLADRSI
                     KDNSASPLGYGGTPQLQLPQDNTGKTLNIEGQDSTIVLKTDIDQGAGALNFNANATVR
                     PENDQSWVGAGIVVAKGKQVNWQVKNPQGDRLSKLGEGTLHINGKGENLGDISVGQGT
                     VILTQQAGENGKKSAFNQVGIVSGRSTVVLNSADQVDPNKIYFGFRGGRLDLNGNNIA
                     FNRIQNSDDGARIVNNHLQKAATLTINGPKPPEATDLKWGTWKENSADIYEYINPHAN
                     NRTDYFTLKGNPNQYMPTNGASNAHWTFLSSNKDAAVKQVLAQKGLEHRYNSFNGFIG
                     ETDNTQHNGRLNVVYDPKASTPPATASEVTWGKGLVAGADIYLFTNPKTKIREYFALK
                     GDPKQPVPRGGLSSEHWEFLAADRNQAINKVLARKNAPIEQEKLNSIYTFSGGLNLNG
                     DLTVKGGKVLLSGRPTPHAYDAINKQDVVYADDWQNRQFKADNMQLNQYAQLYVGRNV
                     SNLQANLSANDHAQLHLGFINEQTPSCYYSEYTGKTSCDTQAVVSNEIFATLPTTQIN
                     GDVSLAEQSQLHIGKANLIGTIQAAATTSIRLANQASWTNTGDSRTGNLVAENGSTIN
                     LNEKFVTGEIPTRFNTLIIDGNFQGNAKINYLTDIAAGKGDHLQVNGLAEGTFTLALR
                     NSGKEAEVVSPLSLLTLTHQAQADKAKVSLENGYVDLGAYRYVLANRSNGYRLYNPLK
                     DAKDRNQSIATAKAELDHAIAEADKQKQEISRLNAEAEKERQAEKNAKQAAANAQSQL
                     SQANSELTRLQQYADYYRRYYPTYYRQIQGQITVAKQKVTQASAALTTAENNAKASAA
                     QIANVEQAVETAQNIAKQVEAKLATLRITAGNSEAILNAEALKLCQENGANCEHLQAH
                     ADTDSSNIQQSDWVSQYANTALSELSAQANSALQIGQDLDRQLFAKHDKFHVWSSIEH
                     QKTEHQSDLYRPYEQQTNLTQLGIELPLANGINAGVMLSRNHANAEFDEGVNGKSNLL
                     MASLYGKWHSENGTFVSLDGSYGKAKNRIDLFGENRFNRHIMAIGANLGHNFDLAGVQ
                     VQPAVGTRYYRFSAQNYKLGEVEVRSPKAHFMAYQAGVKVSKAFDLSSWKVEPSLAAH
                     YVDASSKRLSVAVNDNTFSQRFGRYLKTEVGVGVTKGQWQLSSHLGLLKGNEIGKQHF
                     AGFKLGYSW"
     gene            complement(11828..12832)
                     /gene="fbp"
                     /locus_tag="PXE44_000671"
     CDS             complement(11828..12832)
                     /gene="fbp"
                     /locus_tag="PXE44_000671"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDL5588212.1"
                     /translation="MKTLGEFIIEKQAEYPGAKGELSGILSSIRLAAKIIHREINRAG
                     LSQDILGTAGSENVQGEAQMKLDVFANETMKKALLAREDVAGFASEEDDNFVAFDNER
                     GRNAKYILMTDPLDGSSNIEVNVSVGTIFSIYRRVSPIGTPVTQEDFLQEGRKQVAAG
                     YVTYGSSTMLVYTTGNGVNGFTYDPSLGLFILSHPDLKTPTEGKYYSINEGQYVTFPQ
                     GVKRFIKYCQETDEAIKRPYSSRYIGSLVSDFHRNLLKGGIYIYPTSTVYPKGKLRLL
                     YECNPIAFLAEQAGGMATDGINPILDIKPTELHQRVPFFVGSTSMVKQAGEFMQEFAK
                     "
     gene            12956..13747
                     /locus_tag="PXE44_000672"
     CDS             12956..13747
                     /locus_tag="PXE44_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL5588213.1"
                     /translation="MNNLRIKMISPEIALAKYNELDVLEINHSKVKAKIALQGAQLLS
                     WQPKNTQQDVLWLSEIEPFQTGVAIRGGIPICHPWFGTDKKPAHGTARIQAWNLLEHT
                     SSADGIRVVLGLENQAKLEMILGEVCELYFTHLAPEPAQAALHTYFQVGDITKTEVQG
                     LATQCFDKLTNQEIEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETV
                     LWNPWHNTVSAMTETAYQNMVCVETARINTLLQQNETLGVRILVR"
     gene            complement(13815..14504)
                     /locus_tag="PXE44_000673"
     CDS             complement(13815..14504)
                     /locus_tag="PXE44_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDL5588214.1"
                     /translation="MLQLRKANERGDGSFSWLKSYHTFSFANYYDLKHIHFSHLRVIN
                     EDFIAPNHGFGKHPHDNMEILTYMLKGRVAHEDSMGNKTEVKAGEFQIMSAGTGVFHS
                     EINPDPNETLHLYQIWIIPNQKDVTPRYNQDRFAPKEGATLILSPNAEEGSFKIYQDM
                     KLWRYQYPTAKSEQLALEPNRSYWLQVVKGNLNINGVELSTSDALGIRQEKALEINTQ
                     DDVEFLLFDLV"
     gene            14697..15362
                     /locus_tag="PXE44_000674"
     CDS             14697..15362
                     /locus_tag="PXE44_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDL5588215.1"
                     /translation="MNFTTYFSDENKRRSLILLSLFHIFIIAISNYLVQISFEVQVPL
                     TEFSFPTTWGTLTFPFIFLATDLTVRVFGADLARKIIFVVMFPALVISYVISVLFFET
                     QFQGFSALTEFNFFVFRIALASFAAYLVGQLLDVLVFNSLRQGKRWWLAPTCSTIFGT
                     LIDTFVFFAIAFYKSSDEYMAEHWFTIGSVDYAFKLFVSLLLFLPLYGVILNFIMKKL
                     DLK"
     gene            15448..16251
                     /gene="map"
                     /locus_tag="PXE44_000675"
     CDS             15448..16251
                     /gene="map"
                     /locus_tag="PXE44_000675"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL5588216.1"
                     /translation="MNNIPLRNEEEIQKLRVACKLASDVLVMIAPYVKEGVTTGELDK
                     ICHEYILANGATSASLGYHGFPKSVCISLNEVVCHGIPSDDKKLKKGDILNIDVTVIK
                     DGYYGDNSMMYVVGEPSVRDKRLMAVTLESLYVGIRTVKAGVRLKEIGTAIQKYVEKE
                     GFSVVREYCGHGIGTEFHCDPQVVHYATDDGGVVLKEGMVFTIEPMVNAGKKEVRLMG
                     DGWTVKTKDRSHSAQYEHQIVVTKDGCEVLTIRDEEIAEGRISRIMHNS"
     gene            complement(16367..17781)
                     /locus_tag="PXE44_000676"
                     /pseudo
     CDS             complement(16367..17781)
                     /locus_tag="PXE44_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605131.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
     gene            complement(18088..18738)
                     /gene="thiQ"
                     /locus_tag="PXE44_000677"
     CDS             complement(18088..18738)
                     /gene="thiQ"
                     /locus_tag="PXE44_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein"
                     /protein_id="HDL5588217.1"
                     /translation="MIELDLHFTYEQMNMHFQLSVKKGERIAVVGESGAGKSTLLNLI
                     AGFEKAESQQSQLWLNQKNHLDTEVAQRPVSMLFQDNNIFPHLTVEQNIGLALVPSLS
                     LNPKQKAQVKQIATQMGIETFLQRQADQLSGGQKQRVALARTLLRDKPILLLDEPFSA
                     LDPQRRAELQQIVFDICQSRNLTLLMVTHQLSESLFLFNRVITVADGEIVSDLSPL"
     gene            complement(18735..20309)
                     /gene="thiP"
                     /locus_tag="PXE44_000678"
     CDS             complement(18735..20309)
                     /gene="thiP"
                     /locus_tag="PXE44_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease"
                     /protein_id="HDL5588218.1"
                     /translation="MLSTLTKTTAWFIYFSIIALYVFSLWALMDYHQNDTLFVFSDIT
                     ATVAYSLLQASLSAVISLFLGSLLARSFFYLDFKGKNLLYKTISFIWALPSLVIIFAV
                     IGVFGNSGWLAKLFQQLGWNWQFNLYGLHGIILAHCLFNIPFVMKYYTGGLQLTPSSR
                     FQLAAQLNLTGWNYIQIVELPIIKTLLPYMFMTVFLVCFTSFPIVLMLGGSPKYSTLE
                     VAIYQAVTFEFDFAKALILIGVQFVVGIGLQLVMNYISTSALKKLKQQPNRVEIWKPK
                     PTGLAKLGLQAVIFLQSFAIILPLVSVIWAGLSVSKLSERLLNPTLWQATQYSLYLSL
                     IASIFVITISYLIALEARQLLYTKAKLRYSILTAITLFPLLLPIFLLSVGLFILLMNS
                     ELSSEALLLLVGICNGLTLLPFIYPMLFSAMWNSLTSHDKLAQGLGLKGLRRWWIVEK
                     NQLIRPLVTVFALAMSSSLGSFTVIAFFGNADFSSLPYLLYQQLGSYRTEDAAVTALI
                     LMLFALLPFLFSKDKL"
     gene            20570..20827
                     /gene="ptsH"
                     /locus_tag="PXE44_000679"
     CDS             20570..20827
                     /gene="ptsH"
                     /locus_tag="PXE44_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDL5588219.1"
                     /translation="MYSKDVVITAPNGLHTRPAAEFVKAAKGFASDITVTSGGKSASA
                     KSLFKLQTLGLTQGTTITISADGEDEQKAVDFLVDLIPTLE"
     gene            20949..22670
                     /gene="ptsI"
                     /locus_tag="PXE44_000680"
     CDS             20949..22670
                     /gene="ptsI"
                     /locus_tag="PXE44_000680"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDL5588220.1"
                     /translation="MITGIAASPGAVFGKALVLKEEPIVLNTRKITESEVEAEKAKFF
                     TGREKAAAQLTAIKEKARRTLGEEKEAIFEGHLMILEDEELEEEILSYLTDNLVTADV
                     AASKVIEMQASMLAEIDDEYLKERAGDIRDIGNRLVRNILNMNIVDLGDIQDEVILVA
                     YDLTPSETAQLNLDKVLGFITDVGGRTSHTSIMARSLELPAIVGTNNVTSLVKTGDFL
                     ILDAVNNEVHLNPSEEKIANFKALQEKLNAEKEELAKLKELPAQTLDGHKVDVAGNIG
                     TIRDVEGVLRNGGEAVGLYRTEFLFMDRTQLPGEEEQFQAYKEIVEAMNGQMVILRTM
                     DIGGDKELPYLDLPKEMNPFLGWRAVRIGFTRREILDTQLRAVLRASAFGNLAVMFPM
                     IISVEEIRLLKSIVAELKDQLRAEGHAFDENLKLGIMVETPSAAVNARHLAKEADFFS
                     IGTNDLTQYTLAVDRGNEIIAHLYNPLSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     VRATALLLGMGLDEFSMSGISVPHVKKLIRSINFADAKALADEALAQPTAADIERLVD
                     EFYQKLN"
     gene            22755..23255
                     /gene="crr"
                     /locus_tag="PXE44_000681"
     CDS             22755..23255
                     /gene="crr"
                     /locus_tag="PXE44_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005702996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL5588221.1"
                     /translation="MGLFDKLFGSKQKESQEIKIYAPLTGEIVNIEDVPDVVFSEKIV
                     GDGVAIRPSGDTIVAPVNGTIGKIFETNHAFSIESEEGVELFVHFGIDTVELKGEGFT
                     RLAQEGQTVKVGEPIIKFDLALLEGKAKSVLTPIVISNMDEISNLSKLNGQVVAGESV
                     VLTLTK"
BASE COUNT         6624 a         4551 c         5121 g         7009 t
ORIGIN      
        1 atataaaact ttatcagttt gaggtattta ccgtgtctaa tcaatcatca aacgcaccgc
       61 aaagagaaac gttctccgga cgtggtgcat ttattatggc ggctattggt tccgctgtcg
      121 gcttagggaa catctggcgt ttcccttata ctacttatga aaacggcggt ggggctttta
      181 ttctgccgta tatcattgcc cttttgaccg caggtttacc gctgctgttc ttagattatt
      241 ctatcgggca taaatatcgt ggtggtgcac cgctcgcttt ccgccgtttc agctcgaaat
      301 ttgaaacctt cggttggtgg caagtgctga tcaacgtgat catcgggatt tattatgcgg
      361 tggtactcgg ctgggcagca agttacacct acttctcatt cagcggagcg tggggcgata
      421 aaccggtgga tttctttatc ggtgaattct taaaaatggg cgatattact caaggcgtga
      481 gctttgaatt tgtcggtatg gttacagggc cgttaattgc ggtatggctg attgctattg
      541 ctgtgttggc tatggggatc aaaaaaggga tttctgctgc ctcaaacctg ctaatgccgc
      601 agttgatcgt gatgttctta gtgttggtcg gctattcatt aaccctaccg ggtgctgcga
      661 aaggcttaga tgcgttattt accccaaact gggaaaaatt ggctgatcca agtgtgtgga
      721 ttgccgctta cggtcaaatc ttcttctcac tctctatctg cttcgggatt atgattacct
      781 atgcttcata cctgaaaaaa gaaaccgatt taaccggttc aggcttagtg gtgggttttg
      841 cgaacagtgg ttttgaatta ttagcgggta ttggcgtatt tgcggcactc ggctttatgg
      901 caacggctgc agggcaagaa gtgaacgagg tagcaaaagg cggtatcggc ttggcattct
      961 tcgctttccc aaccattatt aaccaagcac caatgggggc attagttggc gtgctgttct
     1021 ttggttcact cacctttgcg gcattaacct catttatctc ggtaattgaa gtgattattt
     1081 cagcggtgca agacaaactc aattcaggtc gtgtgaaagc cacgctacta gtcggcattc
     1141 cgatgatggt ggtatctgtc ttgctattcg gtacaaccac cggtttacca atgcttgatg
     1201 tgatggataa attcgtaaac caattcggta ttgttgcggt ggcgtttgtt tccttgattg
     1261 cgattgtcac cagcgagaaa ttaagcaccc ttgaaaaaca cctgaacgaa gtttcttcat
     1321 tcaaagtcgg ctttatctgg cgtttatgta tcgttattac ctccggcgta ctggcattta
     1381 tgttattcag cgaaatcgcc aaagtgtatc gtgaaggtta tgaaggctac ccaagctggt
     1441 ttgtaaacac cttcggctgg ggaatggcaa tcggcttagt ggtggtggct tatttgctat
     1501 caaaaatggc gtggaaaaat gaaccaaaag gagatgagta atgagttcag cagcaattat
     1561 taccatggtg gtggcaatga cgattatctg gggcggttta ctttttgcga ttaaacgtct
     1621 gccgaaagaa gaacaataat ttgcaaattt ttgtataaaa aagcccgctt gttagcgggc
     1681 ttttggctat acggtaatcc caaatttctc ttccacaaag gcttggaaat cggtgcaacc
     1741 gccaacgtgg ttattatcca caaaaatttg tggtacagtt gcaaccggct tgccaacacg
     1801 aggctctagg tcttgttttg aaatattttc agcgatcata tcaataaatt taaaatcaaa
     1861 atcgcttaat tcttgtttca ttttctcggc taatgttttt gcacgcacgc agtatgggca
     1921 agataaacgt ccataaattt caacaaacat ataatgttcc ttctataaag taaaagtgag
     1981 cgaattgtcc gttaaacggc tctgtgtaag tgtaacaatt ttttcaacct tttaggataa
     2041 atttcttcac catcacggca agcagttaat gtgatatact ggggcaaatt tacaagtaga
     2101 gaaacagaga tgacccaatt aatgacgttt gaggagcttg atttaagccc tcaattactt
     2161 aaagccttag cacacaaagg ctataaccgc ccaaccgcta ttcaacaagc cacgattccg
     2221 gctgcgttag aaggacgtga tctgctcggt tcagccccta ccggtaccgg caaaaccgcc
     2281 gcttttttac tgccggcaat tcagcattta ttagattatc ctcgccgcaa accaggggct
     2341 cctcgcattt taattcttac cccaacccga gagctggcaa tgcaggtggc ggagcaagcc
     2401 caagcactgg ctaaatttac tcagttgagc attgcgacta ttaccggtgg ggtggcgtat
     2461 caaaatcacg gtgaagtctt taataaaaat caagatattg tggttgctac cccgggtcgt
     2521 ttaatgcaat acattaaaga ggaaaatttt gactgccgag cggtggaagt gctgattttt
     2581 gatgaagcag atagaatgtt gcaaatgggc ttcgggcaag atgcggagaa aattgcggca
     2641 gaaacccgtt ggcgtaagca tacttggttg ttttcagcga ccctcgaggg cgagttatta
     2701 gaggattttg ccgagcgatt attaaatgat ccgattaaag tcgatgccga accaagtcgc
     2761 cgtgagcgta aaaaaattca gcaatggtat taccacgccg ataatgtaga gcataaaacc
     2821 aaattattag ctcgtattat cagcgaattt tcggtggaaa gagcgattgt gtttgtccgc
     2881 cgccgagaag atgtgcgaga actcagcgaa accttgcgta agcggggaat tcgttctact
     2941 tatctcgaag gtgaaatggc tcagacccaa cgtaaccaag ctattaaccg tttaaaagaa
     3001 ggcgtagtga atgtgttagt ggcaaccgat gtggcggctc gaggcattga tatagacgat
     3061 attgatttcg tgctgaacta cgatttacct tatagtgcgg atacttactt gcaccgtatt
     3121 gggcgtaccg ctcgtgctgg caaaaaaggt tcggcaattt cattggtgga agctcacgat
     3181 tataaattac tcggcaaaat taaacgttac accgaagaag tactaaaacc tcgcattatc
     3241 gagggcttag agcctcgcac caaagccccg aaagatggcg agctaaatgt gaccagcaag
     3301 aaagaaaaag cacgcattaa gaagaaaaaa gaggcgaaaa aagaagcggc aaaaccaaaa
     3361 gtaaaagtcc gccacaaaga taccaaaaat atcggtaaac ggcgtaaacc aagtagtgaa
     3421 tcggctgcgg ttaaacaggc ataatgatac aagcggttat tttctgaaga ttttttgcaa
     3481 aaatttcatc aaaaataacc gcttgtttat atctacctta ctgccatttg gaacagaatc
     3541 acttctgctt ggcagctgaa agtgatccac attttcatca gaaacccttc tgcaatttgt
     3601 gtaatttcgc tttgaatttc acaaggttcg gtttcgaccg attttgcttt ttgtgcgaaa
     3661 aattcgaccg cttgttgagc cagttcttcg ctctcataca ccgcttcaaa ggcaatttgg
     3721 cggtctgcat tatccaacat agaatcgcct tttagccggc acatattatg tgcctcagcc
     3781 gctttttgtt gcatcgtttt gctcatatta caccttatcc gtaatttgtt ttaaccaagt
     3841 gtaggattga tgctgttttt gctcatcgaa aatgtagttt accgccataa tttcatcaat
     3901 ttccactcgt gcttttaggg ctttaagttg ttgttcaacg ctttgcgggc taccaattaa
     3961 ggagcattcg gtcatttggc ggacaacggt tttctcttgt cggtatagag cattttgctc
     4021 aaaggcaacc ggcccgaaat gcggcacaat ttctgctttc acaaaatttt cccacacttt
     4081 gtcgtcagat tcgagcggtg gttgcagatt ttgttgggca ttggtcacta catttaagaa
     4141 aaactgggtt tgggtggtcg ccagttgtgt tgcttcttca tcggtttctg ccacaatggc
     4201 attcacacct aaaatcacat aaggtttatc taacacatca gagggcttaa aatgtgagcg
     4261 gtaaatgcgg attgcatcgt ccataaagcg aggggcaaag tgtgaagcga aggcgtaagg
     4321 caagcctcgt tcagccgcta aataggcact ttcagggctt gagcctagaa tataaaacgg
     4381 cacatttagc ccttgtgccg gataggcgga aacagggttt gtaccgtcaa agtagccttt
     4441 taattcgctg atttcatcgg ggaaaggcaa actctgggcg taaggcagag gcggttcgca
     4501 tatcggtgcc gggggctcgc ccaagcccca gttctactcg gtttgggtag agtgtttcaa
     4561 gcgtgccgta ttgttctgcc acaatataag ggctgtggtt gggtaacatc acgccaccgg
     4621 agccgactct taatttttcg gtgtgggaga gggcgtgttg aatcaataat tgcgtggcgg
     4681 aactggcaag atttttcata ttatggtgtt cggcaatcca atagcgttca atgccgatat
     4741 tttctgcgtg ttttgctaat ttcaccatag aatcgaccgc ttgtttggca gtttgccctt
     4801 cacgaatcgg cacaaggttg agaatggata agtttgtcat tttgttctcc gagtttgcat
     4861 ataaaaaagg ctgaatagct ttactattca gcacttgtaa aaccatctcc ttaatatggg
     4921 gacgattatt tcttactcaa gaagaccaca gcgtcaatat agcgttggat aaaatcattg
     4981 gtcgagcttg aatcggaaaa tccttctaga ttaagctggt attgaccatt cacgaaaaac
     5041 gccggtacac cacgaatttg gaagtcttca gctaattgaa cttgtttatt gactaaaccg
     5101 tttactgcaa agctgttgat accgctgtca aactgttctg cactaatgct gttagcgatg
     5161 aagaccgcac gaatatcgtc cattgattta aacgcatctt tttgagcggc ttcaaacaag
     5221 gcggttttta ctttatcttc cgctcctaat gccattgcca atgcccaagc acgggttaag
     5281 ttttcagatt ggcgacctaa gaaattgacg tgatattgca ctaatttgct gtctgcaggc
     5341 aatttcgctt tgatttggct tggaattttg taatttagct caaaatcgta acagtggggg
     5401 cagtagaatg agaaaaattc caccacttct ttttgagccg atggggcttt acgcacctca
     5461 atatactctt tgccggcagt tggatctgct gcaactgcgg ttgcgttaaa tgcaaaaagg
     5521 gcagaaagag caaccagtac ggtttttaat gcaaattttt tcataatgat tccttcaaaa
     5581 atatgataag tgagaaataa gacaaactta atcgtttaaa attccacgag ctcggagcaa
     5641 tgcggtttta aaatcatctt cataatcttt tttaatgccc ggaatcggct caaatttatc
     5701 ggcgtttcgc attttgagct gataaatcaa aacctcgtca cgcagggcat gaaaattatc
     5761 cggctcgctt acttccaccg aaagctgttg cagaatatca attaagtgaa gatcggggtt
     5821 tttccgccaa tgttcgccaa gcagttccaa tagctcttct aaacgtttgc atttcatagt
     5881 atgcctctta aaaaacttgt tgaatagtaa gcggaataat atacttttag ccctcatttg
     5941 gcaaaaaaat tcaggtttgg agagggaaat ggaagagaaa atttcagcgg taattttagc
     6001 cggaggtttg gctcggcgaa tgaatggagc ggaaaaaggg ttgcaactgt tagatcaaaa
     6061 accgttgatt tcgcacctgt tagcccgact ttcgccacaa atcgaggata tttggctcaa
     6121 tatcaatcgt tcggtggaac aatatcaaca actttaccct gaattacctt attaccaaga
     6181 cagcttgccc gattttcaag gcccactaag tggaatgctt tccgggttcg agcagattga
     6241 gagcgactat ttactgtttg tgccttgtga cacgccgttt attccaaccc aactattgca
     6301 caagttacac acggcattac gcattaatca ggctcaaatt gcctatgctc acgatggcga
     6361 ccgcccacac ccaacggttg ccttggtgca tcgttcggtg gcggaaaatt taagagcgta
     6421 tttagcctcc ggcgaacgcc gattattgtg gttttttcaa acccaaaaaa gtgtggcagt
     6481 ggatttctcg gcatataaag ccgcatttcg caattttaat tgtttagaag atttaacggc
     6541 tttcaatcca tatcccatca aaaccctggc gattactggc tatagcggca caggcaaaac
     6601 caccttattg gaaaaattaa tcccaaaatt gaccgcttgc ggtattcggg ttggcttgat
     6661 taagcattcc caccacaatg ttgatattga taaaaaagga aaagacagct atcgcttgcg
     6721 agaagcagga gcaaacccga ctatggtcgt gtgtgatgaa cgttgggcat taatgacgga
     6781 aactaagcag gcagcagaaa ctaaacaagc ggtcgaattt tcacaattaa ttgcaaaatt
     6841 tgacccagag acggtagatt tagttttagt ggaaggcttt aagcacgaaa caatgccgaa
     6901 aattcaacta caccgtaaag aaattgaaaa accgttgccg gcgttagacc agtggacaat
     6961 cgcgaccgca accgattatc cgcttgagcg ggaaaatcgg ttggatatta atgatgttga
     7021 acagcttgct gattttattc ggcagtggct agaggcaagc aaagagccgg ctaattaatt
     7081 gactaaaaag cccctaaagt aagcgattgc ttagtttagg ggctaatagc aattaccagc
     7141 tatatcccag tttgaagccg gcaaaatgtt gtttgccgat ttcgttacct ttcaatagac
     7201 caaggtgaga gcttaattgc cattgacctt ttgtaacacc cacgcccact tcagttttca
     7261 gataacgtcc gaaacgttgg ctaaaggtat tgtcattgac tgccacactc aagcgtttgc
     7321 tgctggcatc gacataatgt gcggcaaggc taggctcaac tttccagctg gagaggtcaa
     7381 aggccttgct gactttcaca cctgcttggt aggccataaa gtgtgctttc gggctgcgga
     7441 cttctacttc acccagtttg taattttgtg cgctgaagcg atagtaacgt gtaccaacag
     7501 caggctgaac ttgtacgccg gctaagtcga agttatgtcc aagatttgcc ccaatcgcca
     7561 taatatgacg gttaaagcgg ttctccccga acaggtcgat acggtttttc gctttaccgt
     7621 agctaccgtc taggctgacg aaagtgccgt tttccgaatg ccatttacca tataggcttg
     7681 ccatcagtaa gttggattta ccgttcactc cctcatcaaa ttccgcatta gcgtggttgc
     7741 gggatagcat cacaccggca ttgataccgt ttgcaagcgg taattctatg cctaactgag
     7801 tgaggttggt ttgttgctca tacgggcgat ataaatccga ttggtgttcg gttttttgat
     7861 gttcgatact gctccaaacg tggaatttat cgtgttttgc gaacagttgg cgatctaagt
     7921 cttgcccgat ttgcaacgca gaatttgcct gtgccgatag ctcggaaaga gcggtattgg
     7981 catattggct tacccagtcg ctttgttgaa tattactact gtcagtgtct gcgtgtgctt
     8041 gtagatgctc acaatttgca ccattctctt ggcaaagttt tagtgcctca gcgtttaaaa
     8101 ttgcttcact atttccagca gttatacgta aggtagctaa ttttgcttct acttgttttg
     8161 caatattttg tgcagtttca accgcttgtt ctacgttagc aatttgcgca gcggatgctt
     8221 tagcattgtt ttctgctgta gtgagtgcag cactcgcttg tgtgactttc tgttttgcta
     8281 ccgtaatttg cccttggatt tggcggtaat acgttggata gtaacggcgg tagtaatcag
     8341 catattgttg caaacgggtt aattcgctat ttgcttggct aagctgagat tgtgcgtttg
     8401 cagcggcttg tttagcattt ttctctgctt ggcgctcttt ctcggcttct gcgttcaaac
     8461 ggctgatttc ctgtttttgt ttatccgctt ctgcaatagc atggtctaat tccgctttcg
     8521 ccgttgcaat cgactgattc cgatctttag catcttttaa cggattgtat aagcgatagc
     8581 cgttgcttcg atttgccagc acataacgat atgcacctaa atcaacataa ccgttttcca
     8641 gtgaaacttt agctttatca gcctgagcct gatgggttaa ggttaataaa cttaacgggc
     8701 taaccacttc agcttctttg ccactgttgc gtaatgcaag agtgaaagtc ccctcggcga
     8761 gaccatttac ttgtaggtga tcgcctttgc cagcagcaat atcggttaag tagttgattt
     8821 tggcattacc ttggaaatta ccgtcaataa tcaacgtatt aaaacgagtc ggtatctcgc
     8881 ctgttacaaa tttttcattc aggttgatgg ttgaaccgtt ttctgccact aaattgccag
     8941 tacggctatc cccagtgttt gtccagcttg cttggttagc taaacgaatg ctcgttgttg
     9001 ctgctgcttg aatggtgcca atcaggttcg ctttgccgat gtgtaattgg ctctgttctg
     9061 ccaagctgac atcaccatta atttgtgttg tgggcagtgt tgcaaaaatt tcattagaaa
     9121 cgaccgcttg tgtatcacag cttgttttac cagtgtactc agaatagtag caacttggag
     9181 tttgctcgtt gataaagcct aaatgtagct gagcatggtc attcgctgac aagtttgctt
     9241 gcaggtttga gacattacga ccaacataaa gctgagcgta ttggtttagc tgcatattat
     9301 cagccttaaa ttgacgattt tgccaatcat cggcataaac tacatcttgc ttattgatcg
     9361 catcataagc gtgaggggtc ggtctgccgg aaagtagcac tttacctcct ttcacagtta
     9421 gatcgccgtt taagttcaaa ccaccagaga aggtataaat actattgagc ttttcttgct
     9481 ctattggtgc gtttttacgg gctaatactt tgttgatcgc ttgattgcga tctgcagcta
     9541 aaaattccca atgctcgctg cttaagccac cgcgaggaac aggttgtttt ggatcacctt
     9601 ttaatgcaaa gtattcccga attttcgttt tcggattggt gaacagataa atatctgcac
     9661 cagctactag ccccttaccc caagttactt cagaggcagt tgccggtggt gtagaggctt
     9721 ttggatcata caccacattc aaacgaccgt tatgctgggt gttatctgtt tcgccaataa
     9781 aaccattaaa gctattgtag cgatgctcca gacctttttg cgcgagtact tgttttactg
     9841 ctgcatcctt gttagaagac aggaaggtcc aatgggcatt acttgcgcca ttcgttggca
     9901 tatattggtt tggattgcct ttgagcgtaa agtaatcggt acggttatta gcatgtgggt
     9961 tgatatattc ataaatatca gcactgtttt ctttccacgt tccccatttt aagtccgttg
    10021 cttcaggggg cttcgggcca ttaatggtga gtgttgcagc tttttgtagg tgattattaa
    10081 caatacgcgc accatcatca ctattttgaa tacgattaaa cgcaatgttg ttgccgttga
    10141 gatctaaacg accaccacgg aagccaaagt aaatcttatt tggatcgact tgatcagcac
    10201 tgtttaatac aacggttgaa cggccgctca caataccaac ttggttaaat gcagattttt
    10261 tgccattttc acccgcttgt tgggtcaaaa tgacagtgcc ttgtccaaca ctaatgtcac
    10321 caaggttttc gcctttaccg ttaatatgta gtgtcccttc gcccaattta gataaacggt
    10381 caccttgtgg gtttttgact tgccaattaa cttgtttacc ttttgccact acaattccag
    10441 ctcctaccca gctttgatca ttttcagggc gaactgttgc attagcattg aaatttaatg
    10501 cgccagcacc ttggtcaata tcggttttta atacgatagt gctatcttgt ccttcaatgt
    10561 ttagggtttt tccggtgtta tcttgcggaa gttgtagctg aggtgtgcca ccatagccta
    10621 atggagaagc gctattgtct ttaattgaac gatcagcaag cgaaaccgtt aagtttttcc
    10681 ctttattacc aatgctactg tctttaccct gtgcagacca ttcaaaaacg gcattttgtt
    10741 gcgcattggt taatgtgccg gcaatatctt cggcaaattg tttatcgtga taatcctgac
    10801 ggataatacc ggcacggttg tagtagttgt ttgcaggcgt accaaaggtg taagtagcca
    10861 gtagtagcca gcgtttcgtt ttctgatcgt agccatacac acctgaaccg ctatcaccac
    10921 gagtaccaaa tgcagttaat gctccatatg ggctattgtt taatgcattt cctctaaaat
    10981 ctagccagta atcggaatta tgtaccgcga ttggtaaatg aactcccccg gttaaatatt
    11041 ggtaagatgt tgaaatttgg ttgtctttat gctcggtatt tttgactatt tgacgaccag
    11101 agcctaagcg agcaaagtga gtaaaacgac tactatcttg gtaggctgaa gcattattac
    11161 ccagatcagt cactgttgca ggtgcgacct cagtgactaa tttattgaga cgtggcactt
    11221 gataatcttg atgttgccca ccttcaacaa ttccatagtc gttacgatca acgactttgt
    11281 aagtgtagtg gtgtgcatcg gcattagtgc ctggggcgcc aaattccaca gtattgtatt
    11341 gtgtattatg ggcaacgctg actaaatatt gcggtgcaac tagagttgct atccctaagt
    11401 tacggtttgc agcactaaaa tctggcatcg gtacatttgg gaagagtgca tctaatgtat
    11461 tgccactttt atctttaatt tcaatattgc ttgcacctac ggtgaattta cccttattct
    11521 ccgcaaaatc acggtagtat tgaagatcga tatcatctct gaaaacagag gctgttgcag
    11581 ataaactaac agcaaatgcc acaaagctgg caaggtaaga aagtggaaag tttttattta
    11641 tcataaaatg ttaataaatt gctaatatta atggagtaaa aaggtggggt attctataga
    11701 taattattta tttggcaaga ggtcttacct gttttttata caaatttaca atcttaagat
    11761 aggcaaaagc agctacggaa tacgcagctg ctttgttgaa agttttattg agtgataagc
    11821 aggagcatta ttttgcaaat tcttgcataa attcgcccgc ttgtttgacc attgaggttg
    11881 aaccgacaaa gaacggtaca cgctggtgta gctctgttgg cttaatatct aaaatcggat
    11941 taataccatc tgtcgccatt cctccggctt gttcggcaag aaacgcaatc gggttgcatt
    12001 catagagtaa acgcaatttc ccttttggat atacggtgga ggttgggtaa atataaatac
    12061 cgccttttaa taaattgcgg tggaaatcgg acaccagcga gccgatatag cgtgaagagt
    12121 atggacgttt gatggcttca tcggtttctt ggcaatactt aataaagcgt ttcacacctt
    12181 gtgggaaagt aacatactgc ccttcattga tggagtaata ttttccttcg gtaggggttt
    12241 ttagatcagg gtgtgaaaga ataaataagc ctaatgacgg atcataggta aaaccgttca
    12301 cgccattgcc tgtggtgtaa accagcatgg ttgaagaacc ataagtaaca taaccggcgg
    12361 caacttgttt acgcccttcc tgcaagaaat cttcttgtgt tactggggtg ccgatagggg
    12421 agacacggcg gtaaatagag aagattgttc ccacagaaac attcacttca atattggagg
    12481 agccatcaag cggatcggtc attaaaatat attttgcatt tctcccacgc tcattatcaa
    12541 aggcaacaaa attatcatct tcttccgatg caaagccggc aacatcttca cgtgcaagta
    12601 acgctttttt catcgtttcg ttggcgaata catcaagttt catttgggct tcaccctgta
    12661 cattttcaga acctgcagta cctaaaatat cttgacttaa accggcacgg ttgatttctc
    12721 gatgaataat tttagcggct aaacgaatag aagataaaat cccacttaat tccccttttg
    12781 cccctgggta ttctgcttgt ttttcaataa taaattcgcc gagtgttttc atttttttct
    12841 ccttcgctat gttagaatgc ccttattata acgattacct ttaaagaggt agcggtttaa
    12901 gctttaattc tgtgatccac ttcacatttt atctttcaag atgaggagca atacgatgaa
    12961 taatctacga atcaaaatga tcagccctga aatagcatta gcaaaatata atgaattgga
    13021 tgttttagag attaatcact ccaaagtgaa ggctaaaatt gcattgcaag gtgcgcagtt
    13081 actgagttgg caacctaaaa atactcagca agatgtatta tggttaagtg aaattgaacc
    13141 tttccaaaca ggtgtggcga ttcgtggcgg cattccgatt tgccaccctt ggtttggtac
    13201 agacaaaaag cctgctcacg gcacagcccg tattcaagcg tggaatttgc tggaacatac
    13261 ttcctcagca gatgggattc gtgttgtttt ggggttagaa aatcaggcaa aactcgagat
    13321 gatactaggc gaagtctgtg agttgtattt tactcactta gcaccggagc cggcacaggc
    13381 tgcattgcat acttattttc aggtcggtga tattacgaaa acggaagtac aaggcttagc
    13441 aacacaatgt tttgataaat tgacaaatca agaaattgaa gtgccatcac ctcgcaagat
    13501 aagtgaaaat gtggattgta tttatccggc acaagcggtg aattttattc aagattttgc
    13561 aaatcagcgc accattaaag ttgagcatat caatgctacc gaaaccgtac tttggaatcc
    13621 ttggcataat accgttagtg cgatgacaga aacggcgtat caaaatatgg tatgtgtgga
    13681 aacagcacgg ataaatacct tacttcaaca aaatgaaacg ctaggagtga gaattttggt
    13741 gcgataatgt ttaatgagag caagcagctg aattagtaat attttttaca aatttaaccg
    13801 cttgtctctt gtatttacac tagatcaaac agtaaaaatt ccacgtcatc ttgagtgttt
    13861 atttcaagtg ctttctcttg acgaatgcct aaggcatcgc tggtggaaag ttcaacgcca
    13921 ttgatgttta gattcccttt caccacttgt agccagtaac tgcggtttgg ctctaatgcc
    13981 agttgctctg atttggctgt tgggtattga taacgccaca gcttcatatc ttggtaaatt
    14041 ttaaatgaac cttcttctgc gtttggcgaa agaatcaagg ttgctccctc tttcggggca
    14101 aatctatctt ggttgtagcg aggggtcaca tctttttggt ttgggataat ccaaatctgg
    14161 taaaggtgta aggtttcatt cggatctgga ttaatttctg aatggaaaac cccggttccg
    14221 gcagacataa tttgaaattc gccagctttt acttcggttt tattacccat actgtcttcg
    14281 tgagccacac gtccttttaa catataagtt aaaatttcca tattatcgtg cgggtgcttg
    14341 ccaaaaccgt gattaggtgc aatgaaatct tcgttaatta cccgtaaatg tgaaaaatgg
    14401 atatgtttta ggtcgtaata attggcaaag gaaaaagtgt ggtagctttt aagccagcta
    14461 aaagaaccat cgcctcgttc attggctttt ctcagttgta acattatcta ttccttatgt
    14521 aagtgttaag ttgtgccatt ttactttatg tcaaaaatag ataaacccta atttaataga
    14581 ttaattgttt cattttttga aataatatga gcttgatttt tatgggaaaa tgtatagaat
    14641 aatgccccct tgagttccct tacctcaagt tttcatttaa cgaaaaagga tacaatatga
    14701 actttactac ttatttttcg gatgagaaca aacgtcgttc tctaatttta ctttcacttt
    14761 tccatatttt tatcatcgca attagcaact atttagtgca aattagcttt gaagttcaag
    14821 taccactaac agaatttagc tttccgacaa cttggggaac gttaacattc ccatttattt
    14881 tcttagcgac cgatttaacc gtgcgtgtat ttggtgcaga tcttgctaga aaaattattt
    14941 ttgttgtgat gtttccggca cttgtcataa gctatgtgat ttccgtttta ttctttgaaa
    15001 cccaatttca aggctttagt gcattaactg agttcaactt ttttgttttc cgtattgcac
    15061 tggctagctt tgcagcttat ttagttgggc aattattaga cgttctagta tttaatagtt
    15121 taagacaagg taagcgttgg tggcttgctc caacctgttc aaccattttt ggtactttaa
    15181 tcgatacttt tgtattcttt gctatcgcct tttataaaag tagcgatgaa tatatggcag
    15241 agcattggtt tactattggt tcggtcgatt atgcgtttaa actctttgtg agcctactgc
    15301 tatttctacc gctttatggt gtgatattaa atttcattat gaagaaactt gatttaaagt
    15361 agaaatctaa actgaggttt ggggattgtg ctgaaaaaag gtataatccc cttacttttt
    15421 atggcagata tagcagagag caatgtaatg aataatattc cattaagaaa cgaagaagaa
    15481 attcaaaaac tacgagtagc ctgtaaactg gcttcagatg ttttagtaat gatcgcccct
    15541 tatgtgaaag agggcgtaac aactggtgaa ttagataaaa tttgtcacga atacattttg
    15601 gcaaatgggg caacttcagc cagtttgggc tatcacggct tcccaaaatc ggtgtgcatt
    15661 tcgttaaatg aggtcgtttg tcacggtatt ccaagtgatg acaaaaagct gaaaaaaggc
    15721 gatattttaa acatcgatgt gacggttatc aaagatggct actatggcga taactcgatg
    15781 atgtatgtag tcggtgagcc aagtgtgcgg gataaacgct taatggcggt tacgctagag
    15841 tccctttatg tcgggattcg caccgtgaaa gcgggggtgc gtttaaaaga aattggtaca
    15901 gcgattcaaa aatatgtgga aaaagaaggc ttttcggtag tacgagaata ttgcggacac
    15961 ggtattggta ctgaattcca ttgcgaccca caagtagtgc attatgctac cgatgatggc
    16021 ggtgtagtgt taaaagaagg aatggtgttt acgattgagc caatggtgaa tgccggcaaa
    16081 aaagaagtcc gcttaatggg tgacggttgg acagtgaaaa ccaaagaccg cagccactcc
    16141 gcacaatatg agcatcaaat tgtagttacc aaagacggct gtgaagtgct gacaattcgt
    16201 gatgaagaga ttgccgaagg gcgtatttct cgaattatgc acaacagcta atttgcaaat
    16261 ttttagcgaa aaatgaccgc ttgcgaaact cattgttaag ctaaggcgtt taaaaacaaa
    16321 agggattaag ctaacactta atcccttttt tgtgatctat ttttgattat ttcgcttgtg
    16381 cttttttcac ccaaatggct gaaatggtaa atgcaactac aaatgaaatt accataccga
    16441 cagaatacat tgcaaggctg tcaggtttaa tcgacggaat acctaagaaa ccggctgcac
    16501 ctaaggcaat tgctttcaca ttaaagaaag cgataaatgc actggctaaa cctgaaccga
    16561 tcatcgccgc aataaaggct tggcggtagc gtaaatttac tccgaacatt gccggttgat
    16621 acctaacagg gctgaaatac ctgacggcac ggctaaacca cgcactttgg catctttcat
    16681 tgcaaaagct acacctaaac aggctgcacc ttgtgcaatg ttagacattg ctgcaatcgg
    16741 gaagataaat gtaccgccgg tttgtgctac ttcagctaat aattgggttt caaccgcaat
    16801 aaaggtttgg tgcataccgg taatcacgat tggagcgtag aatgtaccga aaattgctcc
    16861 accgataaag cctaagctgt catataacca cgttaagcct gctgaaatcg ctgaacctgc
    16921 ttcacgaccc aatggtccga ttacagtgaa agctaaaata ccggcgataa ataatgacat
    16981 cattggcgtc accaagttat ctaaatagct tggcacaaat ttacggaaag ttttctcaag
    17041 ggttgctaac acccaagccg aaacgatagt tggaataacc gtgccttgat aacccacttt
    17101 ttcaatttct aagccgaaaa tattccagta tttgatgtta ccttccatta aggttttggc
    17161 atagttccaa ccgtctgcaa gtgccgggtg aaccaataac ataccaagtg ccgcacctaa
    17221 gaatggatta ccgccaaatt tgcgtgtagc tgagaaaccg agtaatacag gtaagaatac
    17281 aaatggtgcg ttagcaatag tgttgatgaa atcaactaaa tcggaaatat tcggataacg
    17341 gctaacaaca gattcacctt cccagaagaa accgactgcc gttagcattg agtgaatacc
    17401 cattaacaaa ccgccagcca caatagccgg aatgatcggc acgaaaatat cggctaagcc
    17461 ttttaccaaa cgttgtagta atggctgatt tgctgcaccg gcagccgcta cttcagaggt
    17521 ggacatatct gccataccca tttgtttttg catttgggca tacacttcat ttaccgtgcc
    17581 tgatccaaaa ataatttggt attgaccggg agtagagaat tgacctttca cgccttcaat
    17641 attttcaatc gcctctttat caattttgga ttcatcagca atggttaagc gtaaacgggt
    17701 tgcacagtgg gcaagtgtgg taatgttttc tttgccacct aattttgcaa tcgtttgttc
    17761 tgcaattttt ggatagttca tttgaattta cctttcataa aaaataatcg gttgcatttt
    17821 aactaaatcg gtttagctaa aaaagagaag atttaaaata tttccaaatt tgtgaagtag
    17881 atcacatttt ggggaagaga atagcggaat agggaggtaa tattagttac agttcccctc
    17941 tttgctaaag tagggggcga gttctgaaaa aaatgaataa cgctagtatc cgtgcggaac
    18001 atcattcacc gttagattgt tggaattgac atcgttgcgt acgccagcgt ggcgtcccta
    18061 catttagaac ctagcagatc tgctttgcta aagaggggag agatcagaaa caatctcccc
    18121 atctgctacc gttatcaccc gattaaatag aaataggctt tcggatagtt ggtgagttac
    18181 cattaacagg gttagatttc tgctttggca aatgtcaaac acgatttgct gtaattcggc
    18241 tcggcgttgc gggtcgaggg cggagaaggg ttcgtccaat aataaaatcg gcttatcccg
    18301 caacaaagta cgagctaatg ccactcgctg tttttgtccg cccgatagct gatcggcttg
    18361 acgctgaagg aaggtttcaa tccccatttg tgtggcaatt tgtttaacct gtgctttttg
    18421 tttcggattg agcgacaaac ttggtactaa agctaagccg atgttttgtt ctaccgttaa
    18481 gtgcgggaaa atattgttat cttggaatag cattgaaaca ggacgttgtg caacttcggt
    18541 atcaagatga tttttctgat ttagccacag ctggctttgc tgactttctg ccttttcaaa
    18601 accggcaatt agattaagta atgtgctttt gcctgcgcca ctttcgccaa ccactgcaat
    18661 acgttcgcct tttttgacag aaagctgaaa gtgcatattc atttgttcgt aagtgaaatg
    18721 gagatccagt tcgatcatag tttatccttg ctgaataaga atggtagtaa cgcaaataac
    18781 atcagaatga gtgcggtaac ggcagcatct tcggttctat agctaccaag ttgttggtag
    18841 agtaaataag ggagtgagct gaaatcagcg ttgccgaaaa aagcaatgac ggtgaaactg
    18901 cctaagctcg atgacattgc taacgcaaat acggtaacaa gcggtcgaat tagttgattt
    18961 ttttccacaa tccaccaacg gcgaagcccc tttagcccaa gtccttgtgc taatttgtcg
    19021 tgcgaggtga ggctattcca cattgcggag aaaagcattg ggtaaataaa tggcagtaat
    19081 gttaagccgt tgcatatccc gaccagtagc agtaatgcct cgcttgagag ttcactattc
    19141 attaataaaa taaacaaacc aaccgataat aaaaagatcg gcagaagtaa ggggaatagg
    19201 gtgattgcgg tgagaatgct atatctgagc ttggcttttg tatagagtaa ttggcgagct
    19261 tcaagtgcaa tcaaatagct gattgtgatt acgaatatag aagcaattaa acttaagtat
    19321 aaagaatact gagtagcttg ccataaagta gggttgagca aacgctcact gagttttgac
    19381 acgcttagtc ctgcccaaat gacgctgaca agcggtagaa ttattgcaaa actttgcaaa
    19441 aaaataaccg cttgtaagcc gagtttagcc agtccggtag gctttggttt ccaaatttct
    19501 actcggttag gttgctgttt tagctttttg agtgcagaag tcgagatata attcattact
    19561 aattgtaagc caatgcccac tacaaattgt accccaatta agatgagggc ttttgcaaaa
    19621 tcaaattcaa aagtgaccgc ttgataaatg gcaacttcaa gcgtgctgta ttttgggcta
    19681 ccgcctaaca ttaatacgat tggaaaactg gtaaagcaga ccagaaaaac agtcataaac
    19741 atataaggca gcaaagtttt gattatcggt agttccacaa tttggatata attccaacct
    19801 gttaggttta attgagccgc aagctgaaag cgactgcttg gagtcaattg aagccctccg
    19861 gtataatatt tcatcacaaa cggaatgtta aacaggcagt gagccaaaat gatgccgtgc
    19921 aagccgtata aattaaattg ccaattccaa ccgagttgtt ggaaaagttt ggctagccag
    19981 cctgagttac caaatacgcc aattaccgca aaaataatta ctagtgaggg taatgcccag
    20041 ataaacgaaa tggttttata gagtaaattt tttcctttaa agtccaaata aaagaaagat
    20101 cttgccagta ggctgcctaa aaatagtgaa attaccgcag agaggctggc ttgaagtagg
    20161 ctataagcga ccgtggccgt tatatcggaa aaaacaaata aggtatcatt ttggtgatag
    20221 tccattaacg cccatagact aaaaacatag agagcaataa tactaaagta gataaaccat
    20281 gcggttgttt tggttaatgt gctaagcata ttgctccaaa tttagaatat aagtgaaaga
    20341 agtggtagcg aaattattgt aacatttccg cttaagtgtg tgctagaatc ttgatattcg
    20401 acaaatgatt tttaagttag cttaaaaaac accctattta gcagggtgat acgacgagtt
    20461 tttgcgcttt ttagcgtgtt tttaatttga atttgtagta aagttgcgtg cgcaaaataa
    20521 atggattaaa acaaattttt ttaactttca aatttaagag gaagatttta tgtactcaaa
    20581 agatgttgtt attaccgctc caaatggttt acacactcgc ccagcggctg aatttgtaaa
    20641 agcagctaaa ggttttgctt ctgatatcac tgtgacttca ggtggtaaaa gtgcaagtgc
    20701 aaaaagctta ttcaaattac aaacattagg tttgactcaa ggcacaacaa ttacaatttc
    20761 tgcggacggc gaagatgagc aaaaagcagt tgatttctta gttgatttga ttcctacttt
    20821 agaataattt ttctattctc ggatttaaga tttatgtcac tttattgtag cttaataagc
    20881 tacaataaag tgttttgcta tgtaacatcc agatgatttt tacatcatct taaattaagg
    20941 acacaattat gattacaggt atcgcagctt cccctggtgc agtatttgga aaagcattgg
    21001 ttttaaaaga agagccgatt gtactaaata ctcgcaaaat tactgaaagc gaagttgagg
    21061 cagaaaaagc aaaattcttt acaggccgcg aaaaagcagc tgctcaatta accgcgatta
    21121 aagaaaaagc tcgccgtaca ttaggtgagg aaaaagaggc gattttcgaa ggccacttga
    21181 tgatcttaga agatgaagag cttgaagaag aaattttgag ttaccttact gataatcttg
    21241 tgactgcgga tgttgcagca agtaaagtga ttgagatgca ggcttcaatg cttgcggaaa
    21301 ttgacgatga atatttaaaa gaacgtgcag gtgatattcg tgatattggt aaccgcttgg
    21361 tacgcaatat tttaaatatg aatatcgtag atcttggtga tattcaagat gaagttattt
    21421 tagtcgctta tgacttaacc ccttccgaaa cagctcaatt aaatttagat aaagtattag
    21481 gctttattac cgatgtcggt ggtcgtactt ctcacacttc tattatggct cgttccctag
    21541 agcttcctgc cattgttggt actaacaatg ttacttccct agttaaaact ggtgattttc
    21601 tgattttaga tgcagtgaac aatgaagtac atttaaatcc atcggaagaa aaaatcgcta
    21661 atttcaaagc attacaagag aaattaaatg cggaaaaaga agaattagcc aaattaaaag
    21721 agttgcctgc tcaaacatta gatggccata aagttgatgt ggcagggaat atcggtacta
    21781 tccgtgatgt ggaaggtgta ttacgcaacg gtggtgaagc ggttggttta taccgtactg
    21841 agtttttgtt tatggatcgt actcaattac cgggagaaga agagcaattt caagcctaca
    21901 aagagattgt tgaagcaatg aatggtcaaa tggtcattct gcgtacgatg gatatcggag
    21961 gtgataaaga gttaccatac cttgatttac cgaaagaaat gaacccattc ttaggttggc
    22021 gcgcggtgcg tatcggtttc acccgccgtg aaattttaga tactcagtta cgtgcggtat
    22081 tacgtgcttc tgcgttcggt aatttagcgg taatgttccc gatgattatt tcggtggaag
    22141 aaatccgttt attaaaatcc atcgttgcgg aattgaaaga tcaattacgt gcagaaggtc
    22201 acgcttttga tgagaactta aaactaggta ttatggtgga aaccccatca gcagcggtaa
    22261 atgcacgtca tcttgcaaaa gaagccgact tctttagtat tggtactaat gacttaaccc
    22321 aatatacact tgcggtagat cgtggtaatg aaattattgc tcatttatat aacccattaa
    22381 gcccatcagt cttaaactta atcaagcaag ttattgatgc ttcacacgct gaaggcaaat
    22441 ggacaggtat gtgtggtgaa ttagccggtg acgtgcgtgc aactgcgttg ttgctcggta
    22501 tgggattaga tgaatttagt atgagcggta tctctgtgcc acacgttaaa aaactcattc
    22561 gctcaattaa ttttgccgat gctaaagcat tagctgatga ggcgcttgct cagccaactg
    22621 ctgcagatat tgaacgctta gtagatgagt tttatcagaa attaaactaa ttttaattat
    22681 ttagcataca aatacaaaat tctcgtataa aatgtacgag aattttgtat ctaatatcta
    22741 agggagattt taagatgggt ttatttgata aattattcgg ctcaaaacaa aaagagagtc
    22801 aagaaatcaa aatctatgcg ccattaacag gtgaaatcgt aaatattgaa gatgtgcctg
    22861 atgttgtttt ctctgaaaaa attgtaggtg acggtgtggc aattcgcccg agtggagaca
    22921 ctatcgttgc tccagtaaat gggactatcg gcaaaatttt tgaaaccaac cacgcttttt
    22981 cgattgaatc tgaagaaggc gtggaattat ttgttcactt cggtattgat actgttgaat
    23041 taaaaggcga aggctttact cgccttgcac aggaaggtca aaccgtaaaa gtgggcgagc
    23101 cgatcattaa atttgattta gctctacttg aaggcaaagc caaatcagta ttaacaccaa
    23161 tcgtgatttc aaatatggat gaaatcagca acttatctaa attaaatggc caagttgttg
    23221 ccggtgaaag cgtggtttta accttaacca aataatacct cctacttagc tggaagccac
    23281 gatggaagat cgtggctttt ttgtt
//
LOCUS       DANLZC010000022        22904 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.022, whole genome shotgun sequence.
ACCESSION   DANLZC010000022 DANLZC010000000
VERSION     DANLZC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22904
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.022"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            80..673
                     /gene="hisH"
                     /locus_tag="PXE44_000682"
     CDS             80..673
                     /gene="hisH"
                     /locus_tag="PXE44_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDL5588222.1"
                     /translation="MTNLTIIDTGCANLSSVKFAFDRLGIRAEISRDLNKIQSADKLL
                     LPGVGTAAAAMAILHDRRLIDTIRNATQPMLGICLGMQLMTDFSHEGDVATLNLMSGK
                     TERLPECGLPLPHMGWNKVQYQADHPLFHDIEQGNHFYFVHSYGVLPNENTIATCDYG
                     VPFSAAIASQNFYGVQFHPERSGEAGAKLLRNFVENV"
     gene            772..1617
                     /locus_tag="PXE44_000683"
     CDS             772..1617
                     /locus_tag="PXE44_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HDL5588223.1"
                     /translation="MNEIKLFENSQIRSVWDSEKEEWFFSVVDIIEVLTQSSNPRRYW
                     SDLKRKIKDEEGGIELYEKIVQLKLKAPDGKMRETDATDLQGIFRIIQSVPSPKAEPL
                     KMWLAEVGKERIDEIIDPELTIDRALATYLKKGYSREWINQRLQAIQVRKELTDTWQD
                     HGVQVGREFAILTNEITQAWSGMTTRQYKDFKGLKKESLRDNMTTTELVLNMLAEAAT
                     KDIAQISHPQGLEENKTVAKRGGNVAKVARESLEAETGKPVITQQNAIDFAAVIEQVA
                     KQVKK"
     gene            1787..2536
                     /gene="hisA"
                     /locus_tag="PXE44_000684"
     CDS             1787..2536
                     /gene="hisA"
                     /locus_tag="PXE44_000684"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDL5588224.1"
                     /translation="MQKSIIIPALDLIDGQVVRLHQGDYAKQTTYADDPIAQFADYLA
                     QGAEQLHLVDLTGAKNPAQRQTALIGKIIRETHCKIQVGGGIRTEQDVADLLAVGANR
                     VVIGSTAVKEREMVKGWFKKYGAEKFVLALDVNIDAIGQKIIAMSGWQEASGVSLEEL
                     IEDYQTVGLQHVLCTDISRDGTLAGSNVDLYREICAKYPEIQFQSSGGIGSLADIKAL
                     CGTGVAGVIVGRALLEGKFNVAEAIECWQNG"
     gene            2518..3291
                     /gene="hisF"
                     /locus_tag="PXE44_000685"
     CDS             2518..3291
                     /gene="hisF"
                     /locus_tag="PXE44_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDL5588225.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGA
                     DELVFYDITASSDSRTVDKSWVERVAQVIDIPFCVAGGIKTIADAEQIFAFGADKISI
                     NSPALANPELITELAKRFGVQAVVVGIDSWFEAETGKYWVNQYTGDEKRTRQTEWQLL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLVQLKKVREVCQVPLIASGGAGEMVHFRD
                     AFIEANVDGALAASVFHKQIINIGELKEYLAKEGVEVRR"
     gene            3288..3674
                     /locus_tag="PXE44_000686"
     CDS             3288..3674
                     /locus_tag="PXE44_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5588226.1"
                     /translation="MIKITALDHLVLTVADMQKTIDFYTTILGMQEITFGDNRKALLF
                     GTQKINLHQKGAEILPNAQNADCGTADLCLLTVTPLQEVIYVLQRHQVEILEGGIVPR
                     TGAVGKIESVYCRDPDGNLIEISLYV"
     gene            3702..4340
                     /locus_tag="PXE44_000687"
     CDS             3702..4340
                     /locus_tag="PXE44_000687"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDL5588227.1"
                     /translation="MQNKIDWQKVDGLLPVIVQNAQTCEVLMLGYMNQQALEKTLAEK
                     RVTFYSRTKQRLWTKGETSGNFLNVVDMSLDCDNDTLLILANPIGETCHTGAESCFHQ
                     FEQEQPDWIFFSKLERMIAERKNADPESSYTAKLYAKGTPKIVQKVGEEAVESVIAAM
                     KNDREELVGEVADLMYHLTVLLHNADLSWAEINAKLKERHKGIGLHPEGSNK"
     gene            complement(4414..5307)
                     /gene="hslO"
                     /locus_tag="PXE44_000688"
     CDS             complement(4414..5307)
                     /gene="hslO"
                     /locus_tag="PXE44_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL5588228.1"
                     /translation="MNYTKDNDKLYRYLFKDRAVRGEWVRLNNTFTETLNTHHYPKAV
                     QNLLGEMLVATSLLTATMKFNGNITVQIQGDSPLKLAVVNGNDQQQLRALARVQADIA
                     DTASLAEMIGNGVLVISIIPTDGERYQGVIALDKPTIRECLEDYFARSEQLQTHLVIR
                     TGEFEGKAVAGGMLLQIMPDGTGSRDDFEHLATLTETVKDEELFGLEAEELLYRLYHE
                     EQVEVYPPQDTEFKCGCSRERSGSALLLVPAEEIEEMLEEKQGVIDMQCECCGTQYFF
                     DKAAIEGFKEETEKLSKLGLN"
     gene            complement(5551..5967)
                     /gene="hslR"
                     /locus_tag="PXE44_000689"
     CDS             complement(5551..5967)
                     /gene="hslR"
                     /locus_tag="PXE44_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDL5588229.1"
                     /translation="MKNKHEKEQNDAEVRLDKWLWAARFYKTRSIAKAMIEGGKVHYN
                     GQRAKVSKAVEIGAIIKLRQGNEEKEVEVLALSDQRRGAPEAQLLYSETEKSIKHREA
                     MAFARKANALSMPHPDRRPNKKERRDLIKFKEQEFS"
     gene            6055..6597
                     /gene="nudE"
                     /locus_tag="PXE44_000690"
     CDS             6055..6597
                     /gene="nudE"
                     /locus_tag="PXE44_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDL5588230.1"
                     /translation="MIKQLPEILNIKTVAKSRIFEVQAVDLRFSNGEERTYERLTPNR
                     RSSVMVIPIQNNELIFVKEYAVGSERYELGFPKGIVDAGEEPIESANRELQEEIGFGA
                     KKLDFLRSLYSSPSHMYGLMHLFLAQDLYPSKLEGDEPEPLEIVRYPLDKIDQLLADP
                     DFAEARNLSALFMLREYLKK"
     gene            6611..7429
                     /gene="cysQ"
                     /locus_tag="PXE44_000691"
     CDS             6611..7429
                     /gene="cysQ"
                     /locus_tag="PXE44_000691"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDL5588231.1"
                     /translation="MQTLTQPLLENIINIAKAAGEQLKQLYARSVAIKIKADCTPVTE
                     ADLIISQFLTERLKQLTPDIPVLSEEFCDIPFAERQQWQEYWIIDPIDGTQQFINRTG
                     QFSVVIALVQKREGRNQPVLGVIHAPILGKTYFSMEGSGSFLQENGEIRPLVKPKRAE
                     NALLIVTVGSDLHAEQAKNAISAPQIECVKYGSGSLKAGLVAEGKADCYIRFGDTGEW
                     DTAVAEVLLSEMGGKIFDFNFSPLTYNQRESLINPYFVMVADGSIDWDKIFLFK"
     gene            7504..8991
                     /locus_tag="PXE44_000692"
     CDS             7504..8991
                     /locus_tag="PXE44_000692"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL5588232.1"
                     /translation="MNAENSCIVIFGASGDLTFRKLIPALYNLYKIGRLGEHFSVLGV
                     SRSELTDESFRQKMRDALVKFEKASGEELDKFCEHLYYQAVNTSDAVDYAKLLPRLDE
                     LHDKYQTAGNTLYYLSTPPSLYGVIPECLAAHGLTTEEFGWKRIIVEKPFGYDIETAK
                     KLDVQIHKCFEEHQIYRIDHYLGKETVQNLLVLRFSNGLFEPLWNRNFIDYVEITGAE
                     SIGVEDRGGYYDGSGAMRDMFQNHLLQVLAMVAMEPPAIINANSMRDEVAKVLYCLHP
                     LTEEDVKHNVVLGQYARGTVDGKEVPAYLEEKGVPADSNTETFMAVKCKIDNWRWAGV
                     PFYVRTGKRLPTRVTEIVIHFKTTPHPVFSQNAPENKLIIRVQPDEGISMRFGLKKPG
                     AGFEAKEVSMDFRYSDLSSSSSLLTAYERLLLDALKGDATLFARTDAVHACWKFVQPI
                     LAYKANRGRVYEYESGTWGPTEADKLIARHGKVWRKPSGTMKKKV"
     gene            9131..9829
                     /gene="pgl"
                     /locus_tag="PXE44_000693"
     CDS             9131..9829
                     /gene="pgl"
                     /locus_tag="PXE44_000693"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDL5588233.1"
                     /translation="MNKVIFPTAQAAVEQIAKEFIEYSHQNRPVHISLSGGSTPKLLF
                     KTLAQAPFNTEIQWQNLHFWWGDDRMVAPTDPESNYGEVQKLLFDHIDIPSENIHRIR
                     GEENVEQELARFSAELTACVPNLAFDWIILGMGTDGHTASLFPHQTDFNDQNVAVIAK
                     YPETGQIRISKTASLIEKAKRITYLVTGASKAEILKEIQTTPAENLPYPAAKIQAENG
                     VTEWYLDSEAANLL"
     gene            10005..10892
                     /locus_tag="PXE44_000694"
     CDS             10005..10892
                     /locus_tag="PXE44_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDL5588234.1"
                     /translation="MNPIQLIEPNINSKIFTIRGEQVMLDVHLAELYQVDTRTFNQAV
                     KRNEERFPDIFRFQLTENEWLSLRSQFVILNENSGRGQHRKYLPYVFTEQGIAMLSAV
                     LRSEVAVQVSIKIMQAFVQMRHLLQTNSGLVQRLESVERKQLEMSGQLEQVFQSLEGN
                     KPKMQGVFFEGETFNAYQFVTELVRGAKSSLILIDNYVDDTVLTLFTKRSHNVSVTIY
                     TKQISKQLALDLEKYNSQYAHINVKIFDKSHERFLIIDEKELYHIGASLKDLGKKWFA
                     FSKMDLSMSRILEKLAENQ"
     gene            10902..11591
                     /locus_tag="PXE44_000695"
     CDS             10902..11591
                     /locus_tag="PXE44_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDL5588235.1"
                     /translation="MKTLGILGGMSAESTVSYYLDINRSVNKVLGGNSSAKILISSVN
                     FEEIVQCQKQGNWQAAAEILSEQARLLEQAGAEGILLATNTMHKIADQISSHISVPFL
                     HILDCVAEAISAQGLQKVALLGTKFTMSDNFYKDGLLERGITSIVPDEETQQEIHRII
                     FEELCLGKITESAKAFYLQTIQQLSEQGAEGVILGCTEIGLLINQNDNPLPFFDTAAL
                     HSQMAVEFILG"
     gene            11674..13128
                     /gene="gnd"
                     /locus_tag="PXE44_000696"
     CDS             11674..13128
                     /gene="gnd"
                     /locus_tag="PXE44_000696"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HDL5588236.1"
                     /translation="MSQKGDIGVIGLAVMGQNLILNMNDNGFKVVAYNRTTSKVDEFL
                     EGAAKGTNIIGAYSLEDLAAKLEKPRKVMLMVRAGEVVDQFIEALLPHLEEGDIIIDG
                     GNSNYPDTNRRVKALAEKGIRFIGSGVSGGEEGARHGPSIMPGGDESAWQYVKPIFQA
                     ISAKTDKGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFLKDGLGLSYDEMH
                     EIFKEWKNTELDSYLVDITTDILAYKDEDGEPLVEKILDTAGQKGTGKWTGINALDFG
                     IPLTLITESVFARCVSAFKDQRVAASRLFNKEIGKVEGDKKVWVEAVRRALLASKIIS
                     YAQGFMLIREASEQFGWDINYGNTALLWREGCIIRSRFLGNIRDAYEANPYLVFLGSD
                     PYFKDILENCLADWRKVVAKSVEIGLPVPCMASAITFLDGYTSARLPANLLQAQRDYF
                     GAHTYERTDKARGEFFHTNWTGRGGNTASTTYDV"
     gene            13263..13856
                     /locus_tag="PXE44_000697"
     CDS             13263..13856
                     /locus_tag="PXE44_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL5588237.1"
                     /translation="MLTDQDIFNRYQTKKSEFEKQADISLIGHSLFDMWGDMENGTPN
                     LAGKTVANLGLSGTSTRQYLDVITRQERIQHVGDNVFIFLGVNDIVKEPDYSPKQVLE
                     WIGEIVDSLRKINPNANFFLLEATPVNNITTTNNAAIKELNAYFEANIPSDLTYVKTW
                     EYFADKEGNLDLTLCHDGLHFTQAGYDRLEAILTKHL"
     gene            complement(13927..16791)
                     /locus_tag="PXE44_000698"
     CDS             complement(13927..16791)
                     /locus_tag="PXE44_000698"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL5588238.1"
                     /translation="MTKQFTMEDRFNPSAVEQALYQHWESQGYFKPSEDINAPSYCIA
                     IPPPNVTGSLHMGHAFQQTLMDTLIRFNRMEGNNTLWQTGTDHAGIATQMVVERKIAA
                     EEGKTRHDYGREAFINKIWDWKAYSGGTISQQMRRLGNSIDWDRERFTMDEGLSNAVK
                     EVFVRLHEEGLIYRGKRLVNWDPKLHTAISDLEVENKESKGSLWHFRYPLANGAKTAD
                     GLDYLVVATTRPETMLGDTAVAVHPEDERYQALIGKSVILPLANREIPIIADEYVDRE
                     FGTGVVKITPAHDFNDYEVGKRHGLPMVNVMTLNADIRAEAEIIGTDGKPLSDYTAPI
                     PADYQGLERFAARKKIVADFEALGLLDQIKPHDLKVPYGDRGGVPIEPMLTDQWYVSV
                     KPLAEVATKAVEDGEIQFVPKQYENLYFSWMRDIQDWCISRQLWWGHRIPAWYDEQGN
                     VYVARDEAEVRAKHNLPADLPLKQDEDVLDTWFSSGLWTFSTLGWPEQTKELKMFHPT
                     DVLITGFDIIFFWVARMIMFTMHFIKDENGKPQVPFKTVYVTGLIRDEQGQKMSKSKG
                     NVLDPIDMIDGISLDDLLEKRTGNMMQPQLAEKIAKATRKEFAEGIVAHGTDALRFTL
                     TALASNGRDINWDMKRLEGYRNFCNKLWNASRYVLTNDKLDLSEGDVEFSLADRWIES
                     QFNRTVETFRTALSQYRFDLVANAIYEFTWDQFCDWYLELTKPIFFKGTDVQRRGASR
                     TLVNVLEKLLRLIHPVMPFITEEIWQKVKGFVGIEADTIMLQKFPQFDPLAIDETAES
                     QINFIKEVIVAVRNIRAESNIAPSKGLDLIARNFSADEVSILNANEVLLKSMAKLDSV
                     KVLENGENAPLSVAKLVANGEILIPMAGFINKEAELARLTKEMDKLKGEVARIEGKLS
                     NEAFVAKAPEQVIAKEREKMQEYLSGLEKLQVQYQEIEAL"
     gene            complement(16908..17606)
                     /locus_tag="PXE44_000699"
     CDS             complement(16908..17606)
                     /locus_tag="PXE44_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588239.1"
                     /translation="MKQNRKKSKKVIGTHKKENGLKQFIKNNKIPIILISAIWSIFTT
                     DILERIANMPIIWKNFEENYLYNKKLDGLWSTNTNYIIDYKLLNIPEEQPNVIIEFMV
                     NKDDNSILGEMTSTALCELIPLTWSFRLESPKPTLLNLIFDRELKLSYLVSGGYTELS
                     TFKIKEINSNLGTLTLTTQEDKTGIFPPTIKLINFSSNIEDDIKEIEQSCLNSRKQLW
                     EKRRKMQEEQKIPN"
     gene            complement(17851..18444)
                     /locus_tag="PXE44_000700"
     CDS             complement(17851..18444)
                     /locus_tag="PXE44_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588240.1"
                     /translation="MHNNLAIFYCGTNGAGKSTLRSFNQDKVEIVIDSDNIAMQINPL
                     NPRLADIEAGRKAIELFQFAVKNQVAFSMESTLSGKSILKRMENAKAGYFKTRLNYIG
                     VDSYKINIARVKARVKAGGHFIDEETIRKRYHISRENLIEAIFINDETLIYDNSETKP
                     NLILQIADKKVIKISDKIPNWCNQLCDELVELGFKTT"
     gene            complement(18434..18604)
                     /locus_tag="PXE44_000701"
     CDS             complement(18434..18604)
                     /locus_tag="PXE44_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588241.1"
                     /translation="MTSREELLKKQRELDILFTAWFEEKKKHEVLTYRRENGDLIQHY
                     PDGTEKVIEYAQ"
     gene            complement(18606..19348)
                     /locus_tag="PXE44_000702"
                     /pseudo
     CDS             complement(18606..19348)
                     /locus_tag="PXE44_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(19345..19788)
                     /locus_tag="PXE44_000703"
     CDS             complement(19345..19788)
                     /locus_tag="PXE44_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5588242.1"
                     /translation="MLKNLSWRAKRFDELSTQELFEIYHARTAVFVVEQNCPYQEVDN
                     KDLQAVHFFTKNAKNLTAYCRLIPANDGVHIGRVLVVKENRGSGLARELVQKAMDYCG
                     EHFPAQPIHAQAQSYLQGFYESFGFKAVSEVYLEDGIAHLDMVLE"
     gene            complement(19782..20219)
                     /locus_tag="PXE44_000704"
     CDS             complement(19782..20219)
                     /locus_tag="PXE44_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDL5588243.1"
                     /translation="MKQVQFYLLSQADAAELSAAEMQACELAAQAWRLGKRVLIACET
                     EQQALNIDEALWARDPDSFVPHNLSGEITTYPTPIEISWKGKRNAQRRDLLISLQTQL
                     PEFIGSFTQIIDFVPVEETAKAQARDRYKQYRQLGWQLETIQC"
     gene            20458..20901
                     /gene="lysM"
                     /locus_tag="PXE44_000705"
     CDS             20458..20901
                     /gene="lysM"
                     /locus_tag="PXE44_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HDL5588244.1"
                     /translation="MGLFDFVGDIGRKLFNKEEDASKAVTEHLAEDNPGVENVNVTVE
                     NGVAKISGIASTAAAVEKAVLMAGNVAGITEVNIDEVTLPDDVQLASDDELYVIQKGD
                     TLWEIATKAYGNGAKYKAIVEANKEVIKDENKIFPGQKIRIPKGL"
     gene            complement(20955..21806)
                     /locus_tag="PXE44_000706"
     CDS             complement(20955..21806)
                     /locus_tag="PXE44_000706"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL5588245.1"
                     /translation="MKQYLDLCNRIVSEGKWVANERTGKHCLTVINADLTYDVEKGEF
                     PLVTTRRSYWKAAIAELLGYIRGYSNAADFRQLGTKSWDANANENAQWLANPYRKGED
                     DMGLVYGAVGRNFPKPDGGSVDLLRQIVDDLKKGIDNRGEIYTFYHPGAFHMGCLRPC
                     LHSHHFSLLDGTLYLNSTQRSADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIV
                     NAHIYEDQLELMRDVQLKREPLNAPKLIINPDIKSLEDLETWVSLDDFKVEGYEYHPA
                     IQYPFSV"
     gene            21899..22450
                     /locus_tag="PXE44_000707"
     CDS             21899..22450
                     /locus_tag="PXE44_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588246.1"
                     /translation="MKGIMKSILVFSTAVFLAACSSNKSGWTRLNDNDVDQKSYAIAY
                     GATAQTYADRVNESYDINSFMNGVNDWYTKKVTLPIEQIRAITINRMLDHNVYAYYSG
                     VIYAADLQANFNHLDPNCWGLVQPASMTQGIHDAMLDLQKNKVRGDEYIKNGVEQILH
                     LCVKTIAEDENESQSKGKTKKHK"
     gene            22659..22753
                     /locus_tag="PXE44_000708"
     tRNA            22659..22753
                     /locus_tag="PXE44_000708"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22694..22696,aa:Ser,seq:gct)
     gene            22767..22843
                     /locus_tag="PXE44_000709"
     tRNA            22767..22843
                     /locus_tag="PXE44_000709"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22801..22803,aa:Arg,seq:acg)
BASE COUNT         6554 a         4654 c         5088 g         6608 t
ORIGIN      
        1 ataagaaagc ttttgaagga aacagatata ttgagtcaat tttacttcac tataagaaat
       61 acttaactca ggaaactaaa tgaccaacct caccatcatc gacacaggct gtgccaacct
      121 gtcttcggtc aaatttgctt ttgaccgtct tggcattcgg gctgagatct cgcgagatct
      181 caacaaaatc caatcggccg ataaattgct actccccggt gtgggaacag cagcggcggc
      241 gatggcgatc ttgcacgatc gccgacttat cgacaccatt cgcaacgcca cccagccgat
      301 gcttggcatc tgcttaggaa tgcagttaat gacggacttt tcccacgaag gcgatgtggc
      361 aacactgaac ttaatgagtg gcaaaaccga acgcttgcct gaatgcggtt tgccgttgcc
      421 gcatatgggc tggaacaagg tgcaatacca agccgaccat ccgctatttc acgacattga
      481 gcaaggcaat catttctatt ttgtgcatag ctacggtgtg ctaccgaatg agaacaccat
      541 cgccacctgc gactacggcg tgccgttttc cgctgccatt gccagccaaa acttctacgg
      601 cgtacagttc caccccgagc gttcgggcga ggctggggcg aagttgctca ggaattttgt
      661 ggagaatgtc tgattttgtg atctcgatcg aaaagtttga aaatagggct tgatttctcg
      721 ctttatttcg acaaaattac tgttaattta accagtgaaa gaaggtgaga aatgaacgaa
      781 attaaactat ttgaaaacag ccaaatccgc tcggtgtggg atagcgaaaa agaagagtgg
      841 tttttctcgg tggtggacat tatcgaggtg ctgacgcaaa gtagcaatcc acgccgttat
      901 tggagtgatt taaagcggaa aatcaaggac gaagagggcg gaattgaact gtacgaaaaa
      961 atcgtacagt tgaaattaaa agccccagac ggaaaaatgc gggaaaccga tgccacggat
     1021 ttacaaggca ttttccgtat tattcagtct gtgccaagcc cgaaagccga gccgttgaaa
     1081 atgtggctgg cagaggtagg caaagagcgg attgatgaga ttatcgatcc tgaattgacc
     1141 attgaccgag ctttggctac ttacttgaaa aagggatatt cccgtgagtg gattaaccaa
     1201 cgcttgcaag ccattcaggt gcgaaaagaa ctgacggata cttggcaaga tcacggtgtg
     1261 caagtgggca gagaatttgc gattctcacc aatgaaatca cacaggcgtg gagcggtatg
     1321 actactcgcc aatataagga tttcaaaggg ctgaaaaagg aaagtctgcg agacaatatg
     1381 acgaccaccg agttggtgct gaatatgctt gctgaggctg ccaccaaaga catcgctcaa
     1441 atcagccacc cacagggctt ggaagaaaat aaaaccgttg ccaaacgggg cggaaatgtt
     1501 gcaaaagtgg ctcgggaaag tttggaggca gagaccggca aaccggtgat tacgcaacaa
     1561 aatgcgattg atttcgctgc ggtgattgaa caagtggcga aacaggtgaa gaaataagtg
     1621 cggtcggttt tttgcaagtt tttggaagaa tttgaccgct tgtaccccat acgagccgta
     1681 tggggctaga taaaccaagc tactccgtag cttagagagg cagtcgcagc gcgactggga
     1741 ttattcagcc tagcacggct cgtgctaggc acacaggaac aataatatgc aaaaatccat
     1801 cattatcccc gccctcgacc ttatcgatgg gcaagtagtt cgcctacatc agggcgatta
     1861 tgcgaaacag acgacttatg ccgatgaccc gattgcccaa tttgccgact acttggcaca
     1921 aggggcggag cagttacatt tggtcgatct tacaggggcg aaaaatccgg ctcaaagaca
     1981 gaccgcactt atcggcaaaa ttattcgtga aacccactgc aaaattcaag tgggcggcgg
     2041 tatccgcacc gagcaggatg tggcggattt acttgctgtt ggagcaaacc gtgtggtgat
     2101 tggttcgact gcggtaaaag agcgtgaaat ggttaaaggc tggttcaaaa agtacggtgc
     2161 ggagaaattt gtgctggcac tggatgtgaa cattgatgca atcggtcaaa aaatcattgc
     2221 gatgagcggc tggcaagagg caagtggtgt gtcgctcgag gagttgattg aagattatca
     2281 aaccgtgggc ttgcaacacg ttttatgcac cgacatttcc cgagacggca cgctggcagg
     2341 ttcaaatgta gatctctatc gagaaatctg tgccaaatac ccagagattc aatttcagtc
     2401 ctcaggcggt atcggctcgc ttgccgacat caaagcgctt tgtggtacgg gcgtggctgg
     2461 cgtgattgtc ggtcgtgcgt tgttggaagg taaatttaat gtagcggagg caatcgaatg
     2521 ttggcaaaac ggataattcc ttgtttagac gtgcgtgacg ggcaagttgt gaaaggcgtg
     2581 cagttccgca accacgaaat catcggcgac attgtgccac tcgcccagcg ttatgcccaa
     2641 gagggggcgg atgagttggt gttttacgat attaccgcct caagtgacag cagaacggtg
     2701 gataaaagct gggtggaacg tgtggcacag gtgattgata ttccgttctg tgtggcaggc
     2761 ggaatcaaaa ccattgccga tgcggagcag attttcgctt tcggagcgga caaaatctcc
     2821 atcaattccc cagcccttgc caaccccgag ctgattaccg aactggcaaa gcgctttggc
     2881 gtgcaggcgg tggtggttgg tattgatagc tggttcgagg cagaaaccgg caaatattgg
     2941 gtcaatcaat ataccggcga tgaaaaacgc acccgccaaa ccgaatggca attgttggat
     3001 tgggtggagg aagtgcaaaa gcgaggagca ggcgaaatcg tgctgaatat gatgaaccaa
     3061 gacggcgtac gcaacggcta cgatttggtg caactgaaaa aagtgcgtga agtttgccaa
     3121 gtcccactca tcgcctcagg cggtgcaggc gaaatggtgc atttccgtga tgcttttatt
     3181 gaggcaaatg tggatggtgc gttggcagcg agtgtgtttc ataaacaaat tatcaatatt
     3241 ggggagttga aggaatattt ggcgaaggaa ggggttgagg tgcgtagatg attaagatta
     3301 ctgccttaga tcatctggtt ttaaccgttg ccgatatgca aaaaaccatt gatttttata
     3361 cgaccatttt gggtatgcaa gaaatcacct ttggcgataa tcgcaaagcg cttttgtttg
     3421 gcacacagaa aatcaacctc caccaaaaag gtgcagaaat cctacccaat gcccaaaatg
     3481 ccgattgtgg cacggcagat ttatgcctct taacggtaac tccacttcag gaggttatct
     3541 atgtgttaca gcgacatcag gtggagattt tggaaggtgg gattgtgcct cgtacggggg
     3601 cagtgggaaa aattgagtcg gtttattgcc gtgaccctga tggtaatttg attgaaataa
     3661 gcctatatgt ctaaacaagc ggtcaaaaaa aggaagaaaa aatgcaaaat aaaatagact
     3721 ggcaaaaagt ggacggctta ctgcccgtta tcgtccaaaa cgcccaaacc tgcgaagtac
     3781 tgatgttagg atatatgaac caacaagccc tcgaaaaaac gctggctgaa aagcgagtga
     3841 ctttttactc ccgtactaaa caacggctgt ggacgaaagg cgaaacctcg ggcaatttcc
     3901 taaacgtggt ggatatgagt ttggattgtg ataacgacac cctgttaatc ctcgctaacc
     3961 ctatcggcga aacctgccat acgggggcgg agagctgttt tcatcagttt gaacaagaac
     4021 aaccagattg gatctttttc agcaaattag aacgaatgat agccgaacgt aaaaatgctg
     4081 atccggaaag ttcttatacc gctaaacttt acgccaaagg cacgccaaaa atcgtccaaa
     4141 aagtagggga agaggcagta gaaagcgtga ttgcggcaat gaaaaatgat cgggaagagt
     4201 tggtcggtga agtggctgat ctgatgtatc acttaacggt gttattgcac aatgccgatc
     4261 tctcgtgggc agagatcaat gctaaactaa aagaacgcca caaagggatt gggttacacc
     4321 cagaaggctc aaataagtaa aatgcaagcg gtcaaatttc accgtttttt tacaaattgc
     4381 aaaaaagaca agaaacttga ccgcttgttt agcttaattt aaccctaact tacttaattt
     4441 ttcagtttct tccttaaagc cttcaatcgc cgctttgtca aagaaatatt gtgtcccgca
     4501 gcattcacat tgcatatcga tcacgccttg tttttcctcc aacatttctt cgatttcttc
     4561 tgccggcacc agtaataatg cggagcctga acgctctctt gagcagccac atttaaattc
     4621 agtatcttgt ggtgggtaaa cttctacttg ctcttcgtgg tagaggcgat agagcaattc
     4681 ttctgcctct aaaccaaata actcttcatc ttttacggtt tcagtgagcg tggctaaatg
     4741 ctcaaaatca tctcttgagc ctgtgccgtc cggcataatt tgtagcaaca taccgcctgc
     4801 cactgctttg ccttcaaatt cgcctgtgcg gataacgagg tgggtttgta attgctcgga
     4861 gcgggcgaag taatcttcta aacattcacg aatggttggt ttatcgagag caatcacccc
     4921 ttgataacgt tcaccatcgg ttgggataat agaaatcact aatacaccat tgccgatcat
     4981 ttccgctaac gaggctgtgt ctgcaatatc tgcctgaaca cgggctaaag cacggagttg
     5041 ctgttggtcg ttaccgttca caaccgccaa ttttaacggg ctgtcacctt gaatctgcac
     5101 agtaatgttg ccgttaaatt tcattgttgc agtcagcaaa ctggttgcga ctaacatttc
     5161 tccgagtaaa ttttgtactg ctttagggta gtgatgggtg tttaaggtct cggtaaaggt
     5221 gttgtttaaa cgcacccatt cgccccgtac cgctcggtct ttgaataaat agcggtagag
     5281 tttgtcattg tctttggtgt aattcatttt gattcctcgt tgcaagcggt cgattattac
     5341 tcacaaaatt atttctaatt ttgttcgctc ctacggagcc gttggcaaag ccaacgttca
     5401 aaatgcattg cattttgtgg ctaaaaatgt gcaaatcgaa cgctgtaggg gcgaatcaat
     5461 gttcgcccga aataattata taggggcgaa aaatttttcg cccctactta ggatattcaa
     5521 tatagggatt atttttctga ttgcaagggc ttagctaaat tcttgctctt tgaatttgat
     5581 taaatcccgc cgttcttttt tgtttggacg gcgatcaggg tgtggcattg aaagagcatt
     5641 cgctttgcgg gcaaatgcca ttgcctcacg gtgtttaatg cttttttcgg tttcactgta
     5701 gagcagttgg gcttccggag cccctcggcg ttggtcactg agggctaaca cttccacctc
     5761 tttttcttca ttgccttggc gaagtttgat gatggcaccg atctccaccg ctttgctaac
     5821 tttagctctt tgcccattat aatggacttt acccccttca atcattgctt tggcaatcga
     5881 acgggtttta taaaaacgag ccgcccaaag ccatttatct agccgaactt cggcgtcgtt
     5941 ttgttctttt tcgtgtttat ttttcatttt ctgccttaaa atcaaaacaa tagtcgaaat
     6001 attagagcga aagtgagatt tttcaactaa aataactcaa aaaggtgaga taaaatgata
     6061 aaacaacttc ctgaaatttt gaacattaaa acagtagcga aatctcgcat ttttgaggtg
     6121 caagcggtcg atttacgctt ttctaatggc gaagagcgaa cttacgaacg acttacgccg
     6181 aatcgccgtt cttcggtaat ggtgattccg attcaaaaca acgagctgat ttttgtgaaa
     6241 gagtatgcgg tcggctcgga gcgttacgag ctgggttttc cgaaaggcat tgtggatgca
     6301 ggtgaagagc cgattgagag tgcgaaccgt gaattacaag aagaaattgg ttttggggcg
     6361 aaaaaattag attttctgcg gtcgctttac agttcgccaa gccatatgta cggcttaatg
     6421 cacctgtttt tggcacaaga tctgtaccca tcaaaattgg aaggcgatga gcctgaaccg
     6481 ctggaaattg tgcgttatcc gttggataaa attgatcaat tattggcaga tcctgatttt
     6541 gccgaagcac ggaatttgtc cgctctattt atgctacgag aatacttaaa aaaataggca
     6601 aggagctatt atgcaaacgc taactcaacc cctactcgag aatattatca atattgccaa
     6661 agcagccggt gagcagttaa agcagcttta tgcccgctcg gtggcaatta aaatcaaagc
     6721 cgattgcaca ccggtgacgg aagcggatct gattatcagc caatttttaa ctgaacgctt
     6781 gaagcaactc acaccagata ttccggtatt atcggaagag ttttgcgata ttccgtttgc
     6841 cgaacgccaa caatggcaag aatattggat tatcgacccg attgacggca cacaacagtt
     6901 tattaaccgc accggtcaat tttcagtggt gattgcgtta gtgcaaaaac gtgagggtag
     6961 aaatcagccg gtgctgggcg ttattcatgc tccgatatta ggaaaaacct attttagtat
     7021 ggaaggtagc ggtagctttt tgcaagaaaa tggagaaatt cgaccgcttg tcaaaccgaa
     7081 acgtgcggag aatgcccttt tgattgttac cgtaggcagc gatttacatg cagaacaagc
     7141 taaaaatgcc atttcagccc cacaaattga gtgtgtaaaa tatggttcgg gcagtctaaa
     7201 agccggttta gtggcagaag ggaaagccga ttgttatatc cgttttggtg acaccggcga
     7261 atgggatact gctgtggcag aagtgctgtt aagtgaaatg ggcggcaaaa ttttcgactt
     7321 taatttttcg ccgctgactt ataatcagcg agaaagtttg attaatcctt attttgtgat
     7381 ggtggctgac ggctcgatag attgggataa aatattttta ttcaaatagg tagttccgat
     7441 tagcaatttt tatgataatc ttgcacccaa caaaatttta acaattagca ataggataaa
     7501 taaatgaatg ctgaaaatag ctgtatcgtc atttttggtg cttccggtga cttaactttt
     7561 cgtaaattaa ttcctgctct ttataactta tataagattg gcagattggg cgagcatttt
     7621 tccgtcctag gtgtttctcg ttcagaatta accgacgaat ctttccgcca aaaaatgcgt
     7681 gatgcgttag tcaaatttga aaaagcaagc ggtgaagaat tagataaatt ttgcgaacat
     7741 ctttattacc aagctgtaaa tacctctgat gcagtggatt atgccaagtt gctgcctcgt
     7801 cttgatgagc tacacgataa atatcaaaca gccggtaaca cgctttatta cctctccacg
     7861 ccaccaagtt tatatggcgt gattccggaa tgtttagccg ctcacggctt aactaccgaa
     7921 gagtttggct ggaaacggat tattgttgaa aaaccattcg gctacgatat cgaaaccgcc
     7981 aaaaaattag acgtacaaat ccacaaatgc tttgaagagc atcaaatcta ccgtatcgac
     8041 cactatttag gtaaagaaac ggtacaaaac ttactcgttt tacgtttttc taacggctta
     8101 tttgaaccgc tttggaaccg taattttatt gattatgtgg aaattaccgg ggcagaatcc
     8161 atcggcgttg aagatcgtgg cggttactat gatggctcgg gggcaatgcg tgatatgttc
     8221 caaaaccact tattgcaagt attagccatg gttgcaatgg agccaccggc aattattaat
     8281 gctaattcaa tgcgtgatga agtcgcaaaa gtgctatatt gcttgcatcc gttaactgaa
     8341 gaagacgtta aacacaatgt ggtgttaggt cagtatgctc gtggcacggt agacggcaaa
     8401 gaagtgccgg cttacttgga agaaaaaggc gtgccggctg attccaacac tgaaaccttt
     8461 atggcggtaa aatgtaaaat cgataactgg cgttgggcag gtgtgccgtt ctatgtgcga
     8521 accggtaaac gcttaccaac ccgtgtgacg gaaatcgtaa ttcattttaa aactacacca
     8581 cacccggtat ttagccaaaa tgctccggaa aataaattga ttattcgtgt gcaaccggac
     8641 gaaggcattt caatgcgttt cggcttgaaa aaaccgggag caggctttga agcgaaagaa
     8701 gtctcaatgg atttccgcta ttctgattta agttcatcat caagcttact caccgcttat
     8761 gaacgcttac tattagacgc acttaaaggt gatgcaacct tatttgcccg tactgatgct
     8821 gtacacgctt gttggaaatt tgtgcaaccg attttagcct acaaagctaa tcgtgggcgt
     8881 gtgtatgaat atgaatccgg cacttggggg ccaaccgagg cagataaatt aatcgccaga
     8941 cacggcaaag tatggcgaaa accgtccggt acaatgaaga agaaagtgta aatttcagtt
     9001 cccctcttta gcaaagaggg gctaggggag atttggcaga agttatatac gctcatacga
     9061 aatattgaaa tcgtaatcaa atctccccct cccctctttt ctaaagaggg gtgatctaac
     9121 gaggcaaatt atgaataaag ttattttccc caccgcccaa gctgcggttg aacaaattgc
     9181 taaagagttt attgaataca gccatcaaaa ccgcccggtg catatttccc tttccggtgg
     9241 ttcaaccccc aaattattat tcaaaacctt agctcaagca ccgtttaata ccgaaattca
     9301 atggcaaaac ctgcatttct ggtgggggga tgatcgaatg gttgccccaa ccgatcccga
     9361 aagtaactac ggcgaagtgc agaaattact gttcgatcac atcgacattc caagcgaaaa
     9421 tattcaccgc attcgtggtg aagagaatgt agaacaagaa cttgcaagat tttcggcaga
     9481 attgaccgct tgtgtgccga acttggcgtt tgattggatt attttaggaa tgggaacaga
     9541 cgggcatacc gcctcactgt tcccacatca aacagatttt aatgatcaga acgttgccgt
     9601 gatcgccaaa tacccagaaa cagggcagat ccgcatttcc aaaacggcat ctttaattga
     9661 aaaagccaaa cggatcactt acttggtcac aggagcaagc aaagcggaaa ttttaaaaga
     9721 aatccaaacc actccggcag aaaatttacc ttatcctgcc gccaaaattc aggcggaaaa
     9781 tggggtaacg gaatggtatt tggatagtga ggcggctaac ttactctagg tcacaatcag
     9841 tgacctttac aagcggttaa attttagaaa aatgttgcaa agtatttttg tgatattcat
     9901 cgaaaaagca gaaaaatctg ctcgtggtat aggccaaaaa ttggcatagt ctttcttatt
     9961 ttgagatcac aaaagtgatc ttcactgaag aataaggaag aaagatgaat ccaattcaat
    10021 taattgaacc aaacattaac tctaaaattt ttactattcg tggcgagcaa gtaatgctag
    10081 atgttcattt ggcagagctt tatcaggtag atactcgaac ctttaaccaa gcagtaaaac
    10141 ggaatgaaga gcgatttcct gacatatttc gttttcaatt aacagaaaac gaatggctat
    10201 ctttaagatc acaatttgtg atcttaaacg aaaatagtgg gcgtggccaa cataggaaat
    10261 atcttcctta tgtctttacc gagcaaggca ttgcaatgct ttcggcagta ttgcgtagtg
    10321 aagttgcggt gcaagttagc attaagatta tgcaagcctt tgtgcaaatg cgtcatcttt
    10381 tgcaaacaaa tagtggtttg gtacaacgct tagaaagtgt agagcgtaaa cagttggaga
    10441 tgtctgggca gttagagcag gtgtttcaat ctcttgaagg caataagcct aaaatgcaag
    10501 gggttttctt tgaaggtgaa acctttaacg cttatcagtt tgttacggaa ttagtgagag
    10561 gtgcaaaatc atcgctgatt ttaattgata actatgtaga tgataccgtt ttaactttat
    10621 ttacgaaacg atcgcataac gtatcagtta cgatttatac gaaacaaata tctaagcaac
    10681 ttgcattaga tttggaaaaa tataacagcc aatatgctca cattaatgtg aaaatatttg
    10741 ataaatctca cgagcgattt ttgattattg atgaaaaaga gctttatcat atcggtgcat
    10801 cacttaaaga tttaggtaag aaatggttcg ctttttctaa aatggattta tcaatgagcc
    10861 gcattttaga aaagttagcg gaaaatcaat aaggtatatt aatgaaaaca ctcggcattt
    10921 taggtggaat gagtgcggaa agcacggttt cttattattt agatattaat cgctcggtga
    10981 ataaagtatt aggtggaaat agcagtgcca agattttaat ttcgagtgtc aattttgaag
    11041 agattgtaca atgccaaaag cagggaaatt ggcaagcagc cgctgaaatt ttatctgaac
    11101 aagctagatt gctagaacaa gccggagcag aaggcatttt attggcaacc aacacaatgc
    11161 acaaaattgc cgatcaaatt agtagccata tctctgtgcc gtttttgcat attttggact
    11221 gtgtggctga ggcgatttca gctcaaggtc tgcagaaagt agcactcctt ggcacaaaat
    11281 ttactatgag tgataatttt tacaaagacg gtttattgga gcgtggtatc acatcaatag
    11341 taccagatga ggaaacgcaa caagaaattc atcgcattat tttcgaggaa ctctgtttag
    11401 gcaagattac tgaaagtgcc aaagcgtttt atcttcagac tattcaacaa cttagtgaac
    11461 aaggtgcaga aggtgtgatt ttaggttgta ccgaaatcgg gttgctgatt aaccaaaacg
    11521 acaatccgtt accgtttttc gatacagcag cattacacag ccaaatggcg gtggagttta
    11581 ttttaggtta ataaatgtta caagcggtca aatttgcaaa aaattttacc aattcgaccg
    11641 cttgcataga atcaaaaaca ataggagcaa acaatgtcac aaaaaggcga tatcggcgtg
    11701 attggactcg ccgtaatggg tcaaaattta attttgaata tgaatgacaa cggctttaaa
    11761 gtggtggcgt acaaccgcac tacttcaaaa gtggatgagt tcttagaagg tgcggcgaaa
    11821 ggcacgaaca ttatcggtgc ttactcgtta gaagatttag cggcgaagtt ggaaaaaccg
    11881 cgtaaagtga tgctaatggt gcgtgccggt gaggtggtgg atcagtttat cgaggctctt
    11941 ctgccacatt tagaagaagg cgacatcatc attgatggcg gtaactcaaa ctatccggac
    12001 accaaccgcc gtgtgaaagc gttagcggaa aaaggcattc gctttatcgg ctcaggtgta
    12061 tcgggcggcg aagagggtgc tcgtcacggg ccgtcaatta tgccgggcgg tgatgaatct
    12121 gcgtggcaat atgtgaagcc gattttccaa gcaatttcag ccaaaaccga caaaggtgag
    12181 ccttgttgtg actgggttgg tcgtgacgga gcagggcatt ttgtgaaaat ggttcacaac
    12241 ggtatcgaat atggcgatat gcagttaatt tgtgaagcct accaattctt aaaagatggt
    12301 ttaggtttaa gctatgatga aatgcacgaa atctttaaag agtggaaaaa caccgaatta
    12361 gacagctatt tggtggatat taccactgat attcttgcct acaaagacga agacggcgag
    12421 ccattggtag agaaaattct cgacaccgca ggtcaaaaag gaacgggtaa atggactggt
    12481 attaacgccc ttgatttcgg cattccatta acactgatta ccgagtcggt attcgcccgt
    12541 tgcgtatctg cgtttaaaga tcaacgtgtt gcggcttcaa gattgtttaa caaagaaatt
    12601 ggtaaagttg aaggcgacaa aaaagtatgg gtagaagcgg tacgccgtgc gttacttgct
    12661 tccaaaatca tctcttatgc acaaggcttt atgttgattc gtgaggcttc tgagcaattc
    12721 ggttgggaca tcaactacgg caataccgcc ctattatggc gtgaaggttg tattatccgc
    12781 agccgtttct taggcaacat tcgtgatgcg tatgaagcca acccatattt agtgttctta
    12841 ggctcagacc catacttcaa agatattttg gaaaactgct tggcagattg gcgtaaagta
    12901 gtggcaaaat cggtggaaat cggtttacct gtaccttgta tggcgtcagc cattaccttc
    12961 ttagatggct acacctcggc acgtttaccg gcaaacttac tccaagcaca acgtgactac
    13021 tttggtgctc atacttatga gcgtacagac aaagctcgcg gtgagttctt ccacaccaac
    13081 tggacaggac gtggcggtaa taccgcatcg accacttatg atgtgtaatt aatctttttg
    13141 agccacttaa ttcgatatta agtggctttt ttattggatt tttcttcaaa atatcttgga
    13201 gtgaatgttt acaagtttgt ataataatgg agcgattttt catttatatt taaggagaac
    13261 ctatgcttac cgatcaagat atttttaatc gctaccaaac aaaaaaatca gaatttgaaa
    13321 aacaggcaga tatttccctt attgggcatt ccctgtttga tatgtggggc gatatggaaa
    13381 acggcacgcc gaatttagca ggaaaaacag ttgccaattt gggcttatca ggcaccagca
    13441 cccgccaata tttggatgtg attacccgac aagagcgaat tcaacacgtt ggcgataatg
    13501 tatttatttt tctaggcgtt aatgacattg taaaagagcc ggattactcg ccaaaacaag
    13561 tgttagagtg gattggcgaa attgtggatt ctcttcgcaa aattaatcca aatgccaact
    13621 tttttctatt ggaagccaca ccggttaata atattaccac caccaataat gctgcaataa
    13681 aagagctcaa tgcgtatttt gaggcaaata ttccatctga tttgacttat gtaaaaacgt
    13741 gggaatattt tgctgacaaa gaagggaatt tagatctaac tttatgccac gatggattac
    13801 acttcaccca agcaggctat gaccgcctag aggcgatttt aaccaaacat ttataatgaa
    13861 aagcttcaat aagcaagcgg tctgaattag caaaaaattt ccaaatcctg accgcttgtt
    13921 agttaattac agtgcttcaa tctcttgata ctgcacctgc aatttctcta atccactcag
    13981 gtactcttgc attttttcac gctctttggc gatgacttgc tctggggctt tggcgacaaa
    14041 ggcttcgttt gaaagtttgc cttcaatgcg agccacttca cctttaagtt tatccatctc
    14101 tttggtgagg cgagctagct cagcttcttt gttgataaag cctgccattg ggatcaagat
    14161 ctcaccattt gcgaccagtt tcgctaccga tagcggtgca ttttcaccat tttccagcac
    14221 tttcacgcta tcaagttttg ccatcgattt gagcaacact tcgttagcgt tgagaataga
    14281 gacttcgtcc gcagagaaat tacgagcgat taaatctaaa cctttgcttg gtgcgatgtt
    14341 gctttccgca cgaatgttac gcaccgccac aatcacttct ttgataaagt tgatttgact
    14401 ttcggcggtt tcatcaatgg caagcgggtc aaattgtggg aatttttgca acataatggt
    14461 atcggcttca atacctacaa agcctttcac tttctgccag atttcttcgg tgatgaacgg
    14521 cattactggg tggattaaac gcaggagttt ttccaataca ttcaccagcg tgcgacttgc
    14581 gccacggcgt tgtacatctg tgcctttgaa gaaaataggt ttggtcagct ctaaatacca
    14641 gtcgcagaat tgatcccaag taaattcgta gatcgcattt gccactaagt cgaaacggta
    14701 ttggctgagt gcggtgcgga aagtttccac cgtgcggttg aattggcttt caatccaacg
    14761 atcggcaagg ctaaattcca cgtcgccttc gcttaaatcc aacttgtcat tggtcagcac
    14821 ataacggctc gcattccaca gtttgttgca gaagttgcgg tagccttcta aacgtttcat
    14881 atcccagttg atgtctcgac cgttgctggc aagggcggtg agggtgaaac gcagagcgtc
    14941 tgtgccgtgt gccacaatcc cttcggcaaa ttctttgcgg gttgccttag cgattttttc
    15001 tgccagttgt ggctgcatca tattacctgt acgtttttcg agcaaatcgt cgagactgat
    15061 gccgtcaatc atatcaatcg ggtctaacac gttccctttc gatttcgaca ttttctgccc
    15121 ttgctcgtca cggatcaagc ctgttacata cacggttttg aacggcactt gcggtttgcc
    15181 gttttcatct ttgatgaagt gcatcgtgaa cataatcata cgagccaccc agaagaagat
    15241 gatgtcaaaa ccggtaatca gcacatcggt cgggtggaac attttcagtt ctttggtttg
    15301 ttctggccag cctaacgttg agaacgtcca tagaccagac gagaaccaag tatccagtac
    15361 gtcttcatct tgctttaatg gtaagtccgc aggtaagttg tgtttcgcac gcacttccgc
    15421 ttcatctcgt gctacataaa cattgccctg ctcgtcatac cacgctggaa tgcggtgtcc
    15481 ccaccataat tggcgagaga tacaccagtc ttgaatatca cgcatccaag agaaatacag
    15541 gttttcgtac tgtttcggca cgaattggat ttcgccgtct tccaccgctt tggtcgcgac
    15601 ttccgccaaa ggtttgacag acacatacca ttggtcggtc agcatcggct caatcggcac
    15661 gccaccacgg tcgccatacg gcacttttaa gtcgtgtggt ttgatttgat ctaacagccc
    15721 aagagcttca aaatctgcca caattttctt acgcgcggca aaacgctcta agccttgata
    15781 gtcggcagga attggggcgg tgtagtcgga aagcggttta ccgtctgtgc cgataatttc
    15841 ggcctctgca cgaatatcgg catttaaggt catcacattc accatcggca agccgtggcg
    15901 tttgcccact tcgtagtcgt tgaaatcgtg ggcaggggtg atttttacca cgcccgtacc
    15961 gaactcacga tccacatact catcggcaat aatcgggatt tcacggttgg caagcggtag
    16021 gattacgctt ttgccaatca gagcttgata acgctcatct tcagggtgaa ccgccaccgc
    16081 agtatcgccc aacatcgttt caggacgggt cgttgccacc actaagtaat ccaagccgtc
    16141 tgcggttttt gcaccattag ccagcggata gcggaaatgc cacagcgagc ctttgctttc
    16201 tttgttttcc acttccaagt cagaaatcgc agtatgcagt ttcggatccc agtttacgag
    16261 gcgtttgcca cggtaaatca agccttcttc gtgcaagcga acgaacacct cttttaccgc
    16321 attggataag ccttcgtcca tcgtaaaacg ctcacgatcc cagtcaatcg agttgcctaa
    16381 gcgacgcatt tgctggctga tcgtgccgcc cgaataggct ttccaatccc agattttgtt
    16441 gataaaggct tcacgcccat aatcgtggcg ggttttacct tcttccgctg cgattttacg
    16501 ctccaccacc atctgagtgg caatccctgc gtggtcagtt cctgtttgcc aaagggtatt
    16561 gttgccctcc atacggttaa aacgaatcag cgtatccatc aaggtttgct ggaaagcgtg
    16621 ccccatatgc aaactccccg tcacattcgg cggcggaata gcgatacagt agctcggtgc
    16681 attaatatct tcactcggct taaaatagcc ttgcgattcc cagtgttggt agagcgcttg
    16741 ctctacagcg gaagggttaa aacggtcttc cattgtgaat tgttttgtca ttggttttgt
    16801 tttctcttgt ttaataattt tttcactagc ttaatcttat aaaaaagatc tttgccgtaa
    16861 atgcttttat aggtaattag ttatttaaat taaggtcaac taaaagatta attgggaatt
    16921 ttttgttcct cttgcatttt tcttcgtttt tcccataatt gctttcggct atttaaacag
    16981 ctttgctcaa tctctttaat atcatcttct atattggatg aaaaattgat taatttgatt
    17041 gtcggaggaa atataccggt tttatcttct tgcgtagtta atgttagtgt tcctagatta
    17101 ctgttaattt ctttaatttt aaatgttgat agctcggtat atcccccact aacaagataa
    17161 ctcaatttca actctctatc gaaaataaga tttaataatg ttggcttagg gctttccaat
    17221 ctaaaactcc aagttagagg tattaactca cacagagctg ttgaagtcat ttcccctaaa
    17281 atcgaattgt catctttatt aaccataaat tcaatgataa catttggttg ctcttcaggt
    17341 atgttcaata atttatagtc gataatgtaa tttgtatttg tagaccataa accatccagt
    17401 tttttattat ataggtaatt ctcctcgaaa tttttccaaa taataggcat gtttgcgatg
    17461 cgttctaaaa tatcagttgt gaagatactc catatggcag atataagaat aataggaatt
    17521 ttgttatttt ttatgaactg ttttaagcca ttctcttttt tatgagtacc aataactttt
    17581 ttagactttt tcctattctg tttcatcttt gtgtttttgt tccaatatcc ccgatggtga
    17641 tcgtcttcga cgagagctta tttatttaag aacctatcag agataggcat aagcggtctg
    17701 tttcgagaag ttttttgcaa agtttttctt taatccgaca gcttgtcacc cacacacaag
    17761 ccgtgtgggg ttacgcatag ctgagccact ttgtggctct aagttacagc tccaaaggag
    17821 ctgggattgt cgagcctagc acggctcgtg ctaggtggtt ttaaacccta attccactaa
    17881 ttcatcacaa agctgattac accaattcgg tattttgtct gaaattttta tcactttctt
    17941 atcagcaatc tgcaaaatca aattcggctt ggtttctgaa ttatcataaa tcagtgtttc
    18001 atcattgata aaaatcgctt caattaaatt ttcacggcta atgtgatagc gttttctaat
    18061 ggtttcttca tcaataaaat gtccgccggc tttcactctt gcttttaccc gtgcgatgtt
    18121 aattttatag ctatcgacac caatataatt taatcgagtt ttgaaatatc ctgctttggc
    18181 attttccatt cgttttagaa tagatttacc cgagagggtt gattccattg aaaatgcaac
    18241 ctgatttttt actgcaaact gaaatagttc gattgctttt ctgcccgcct caatatcggc
    18301 caatcttgga ttgagtgggt taatctgcat cgcaatatta tcggaatcaa tcacaatctc
    18361 gactttatct tgattgaaag aacgtaaagt agatttgccg gctccgtttg tgccacagta
    18421 aaaaattgct aggttattgt gcatattcaa tcactttttc cgtaccgtcc ggataatgct
    18481 gaattaaatc cccattttct cggcgatacg tcagcacttc gtgctttttc ttttcttcaa
    18541 accatgctgt aaagagaata tccagttcac gttgtttttt cagtaattct tcacgacttg
    18601 tcattttatg tcctctgttt cagaatcgcc aaataccttt ccaactcagc taattcatac
    18661 tgactaatca attcaaccaa actactaata tcttgttgat ttttatggaa atcatcaaaa
    18721 cattggtaat attgggctcg attggtaaat ttaatatcaa tcggtaaata gccctgtttt
    18781 aataattcaa aattaatcag taatcgccct gtgagcccgt ttccatcaat aaaagggtga
    18841 atgctttcaa aggctaaatg cagccaactg attgcctcta tgggatgaag tgcggtcagt
    18901 ttttgttgat atttttcaat caataattgc atttcttcgg caattaaata aggggggggg
    18961 cattttctgc ccctaaaatt cgtactggaa ttttgcgata aacccctcgg ctttccgcat
    19021 tgcccattaa taccagagaa tgaatatctt taatcgctcg ttcgcttaat ggttctttat
    19081 tggcgactaa ttcataaata tagttgaatg catccttaaa accgataata tctaaatgct
    19141 cccgaatcgg tttttcggca atggtcattc cctcttttaa aatgagcacc gtttcacgca
    19201 aagtaatggt attgccttca attgcattgg aattatagct gctttcaata ataaactctt
    19261 cccgcagtcg ttgaacttcg ctttcagata atggacggcg ttgtgaaagt gctgttttta
    19321 attgttcgat tttagctaat ttattcattc taacaccata tccaaatgcg caatcccatc
    19381 ttccaaatac acttctgaaa ccgctttaaa cccaaaactt tcataaaacc cttgcaggta
    19441 actttgggct tgggcgtgaa tgggttgagc aggaaaatgt tcgccacaat aatccatcgc
    19501 tttttgtacc aattcccttg ctaagcctga gccacgattt tctttcacca ccaacactct
    19561 acctatatgc acaccatcat tggctggaat taaacggcag taagcggtca gattttttgc
    19621 attttttgta aagaaatgca ccgcttgcag gtctttatta tcgacttctt ggtaagggca
    19681 gttttgctca accacaaaca ccgctgtacg tgcgtggtaa atctcaaaca actcttgggt
    19741 ggagagttca tcaaagcgtt ttgcgcgcca agagaggttt tttagcattg tatcgtttcc
    19801 agttgccaac caagttggcg gtattgttta tagcggtcac gagcctgtgc tttggcggtt
    19861 tcttccaccg gcacgaaatc aataatctgg gtaaaactgc cgataaattc ggggagttgg
    19921 gtttggagtg aaatcaacaa atcccgacgt tgagcattgc gtttaccttt ccacgaaatt
    19981 tcgataggcg ttgggtaagt ggtgatttct cccgataaat tgtgcggcac gaacgaatcc
    20041 ggatctcttg cccaaagtgc ttcgtcaata tttaacgctt gttgctcggt ttcgcatgca
    20101 atcagtaccc gtttacctaa tcgccacgct tgagccgcaa gttcgcaggc ttgcatttca
    20161 gcagcggaaa gctcggctgc atcggcttgg cttaatagat aaaattgtac ttgtttcaca
    20221 gaatgttccc agaaattcaa tcagatattt aaacttaata ttctagctga aaatgagtaa
    20281 aatataaaat ctaagcgttt gtttttgcaa aaaattgaga aaaaatgacc acttgtgttg
    20341 cgttgccttg aggaactaat tagcttttag acgttctaag agaacaatgc actatttcaa
    20401 tagttggaaa ttgtgtagaa tacccacttc atttagctat tttataagga acttactatg
    20461 ggcttatttg attttgttgg cgacattggt cgtaaattat ttaataaaga agaagatgct
    20521 tcaaaagcgg ttacagagca tttggcggaa gacaatccgg gcgttgaaaa tgtaaacgta
    20581 acggttgaaa acggggtagc gaaaatttca ggcattgctt caaccgcagc agctgttgag
    20641 aaagcggtat tgatggcggg taatgtggct ggtatcactg aggtaaatat tgatgaagtg
    20701 accttgcctg atgacgtgca acttgcaagt gatgatgagc tttatgttat ccaaaaaggg
    20761 gatacgttgt gggaaatcgc cacaaaagct tatggtaacg gtgcaaaata taaagcgatt
    20821 gtagaagcaa ataaagaagt tattaaagac gaaaataaaa tcttcccagg gcaaaaaatt
    20881 cgtatcccga aaggtttata atcaaattga aagagctaag ctggtcgaaa gtttagctct
    20941 ttttgtaggt ggtgttagac tgaaaatgga tattgaatgg cagggtggta ttcataacct
    21001 tcaactttga aatcatctaa gctcacccaa gtttctaaat cttctaagga ttttatatca
    21061 ggattaatga ttaatttagg tgcattgagt ggttctcgct taagttgaac atcacgcatt
    21121 aattctagtt gatcttcata aatatgcgca ttgacaattt tatggaacgc ttgtcctgcc
    21181 ttatgccctg taatttgagc cattaaagct aaaaaggtat agcattgaat catattccaa
    21241 tttaacccaa gcggtacatc tgccgaacgc tgagtactat tcaggtaaag cgtgccatca
    21301 agtagagaaa aatgatggct gtgtaaacaa ggacgtaagc agcccatatg gaaagcgccg
    21361 gggtggtaga aggtataaat ttcaccacgg ttatcaattc cttttttcaa atcgtccaca
    21421 atttgtcgca ataaatcgac tgagccaccg tctggtttcg ggaaattacg ccctactgcg
    21481 ccgtaaacca agcccatatc atcttcccct tttcgatagg gattagcaag ccattgtgca
    21541 ttttcatttg cattagcgtc ccacgatttt gtgcctaatt gacggaaatc agcagcatta
    21601 ctatagcctc gaatatagcc taaaagttcg gcaatcgcag ctttccaata actacggcga
    21661 gtggtaacta aaggaaattc tcctttttct acgtcataag ttaagtcagc attaatgact
    21721 gtgaggcagt gtttgcctgt acgttcattt gcaacccatt tgccttcgct aacgatgcgg
    21781 ttacataagt ctaaatactg tttcattatt tacctcataa aaaattttag gtaattttgc
    21841 catttattcg gtaaaatagc taaaacttta tgacacaaca caataaggag ttatggatat
    21901 gaaaggtata atgaaatcca ttctggtttt ttccaccgct gtttttttag cagcttgttc
    21961 atcgaataaa agcggttgga cacgtttaaa tgataatgat gttgatcaga aatcttatgc
    22021 tattgcttat ggtgcaacgg ctcagactta tgcggataga gtaaatgaaa gctatgatat
    22081 caattccttt atgaatgggg taaatgattg gtacactaaa aaggttacat tgccaattga
    22141 gcaaattaga gcaataacga ttaatcgaat gttagaccat aatgtgtatg cttattatag
    22201 tggtgtgatt tatgccgcag atttgcaggc aaatttcaat catttagatc ctaattgttg
    22261 ggggcttgtt caaccagcga gtatgaccca gggcattcac gatgcgatgt tagatttaca
    22321 gaagaacaaa gttcgtggag atgagtatat caaaaatggg gttgagcaaa tattgcacct
    22381 ttgtgtaaaa acgattgctg aagatgaaaa tgaatcgcaa tctaaaggta aaaccaaaaa
    22441 gcataaataa gttaagctat gacaagcggt aaaattttta aaaaatttta ccgctttttt
    22501 ataaccgtat gaaatgttta atatttattc tatgaaactt aaaaaaacag cgttttgttt
    22561 attcaatagg caaacactcg ggatttttca attttcagga aaaagaattt gacatctttt
    22621 taaattctag taatatggcg cccacagaca cagtgagtgg tgagatggcc gagcaggctg
    22681 aaggcgctcc cctgctaagg gagtataggg ttaaaaagct ctatcgaggg ttcgaatccc
    22741 tctctcaccg ccatttactt tatcacgcac ccgtagctca gctggataga gtactcggct
    22801 acgaaccgag cggtcagagg ttcgaatcct ctcgggtgcg ccattttaat ttttactcta
    22861 ttcaaattaa aatggttgct gataaaatta aataaactta caat
//
LOCUS       DANLZC010000023        22280 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.023, whole genome shotgun sequence.
ACCESSION   DANLZC010000023 DANLZC010000000
VERSION     DANLZC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22280
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.023"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            20..742
                     /gene="arcA"
                     /locus_tag="PXE44_000710"
     CDS             20..742
                     /gene="arcA"
                     /locus_tag="PXE44_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDL5588247.1"
                     /translation="MKIPQILIVEDELVTRNMLKSIFEGEGYQVFQASNGEEMHSVLS
                     TNEINLVLMDINLPGKNGLILAREIRENMKLPLMFLTGRDNEIDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLQRTMIDKENDVVKEQSEIESYRFNGWILDINSHSLISPEGEV
                     NKLPRSEFRALLHFCQHPGKIQTREDLLKKMTGRELKPHDRTVDVTIRRIRKHFEDHP
                     NTPEIIVTIHGEGYRFCGNIEP"
     gene            762..1280
                     /locus_tag="PXE44_000711"
     CDS             762..1280
                     /locus_tag="PXE44_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDL5588248.1"
                     /translation="MKKLTIVALTCLFSTHLLAAPVGNTFTGIGVGVDVTTTKYKVEG
                     VKGKDSTGPALVVDYGIEQGNNFVGVVQGKAKVGSTKVFSDVKQKNKYTIAYQQGYRV
                     GSDLLPYVKVDASSSKIGNETYRGFGFGAGAKYAVSSDVELGAEYTRDNLKRSGTKLK
                     GNAFTANATYRF"
     gene            complement(1358..2587)
                     /gene="serA"
                     /locus_tag="PXE44_000712"
     CDS             complement(1358..2587)
                     /gene="serA"
                     /locus_tag="PXE44_000712"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL5588249.1"
                     /translation="MAKISLDKSKIKFLLLEGVHQNAIDVLLAAGYTNIEVHKKALDG
                     QELIDAIKDAHFVGIRSRTFLTEEVLAQAPKLIAIGCFCIGTNQVDLNAAKRRGIPVF
                     NAPFSNTRSVAELVLAEMILLMRKVPTANAEVHRGVWNKSAAGANEVRGKKLGIVGYG
                     HIGSQLSILAEAIGMQVYFYDVENKLPLGNAQQVPTLEHLLSTCDMISLHVPEIDSTK
                     NLMSAERIAQLKEGSVLINAARGTVVDLDALAARIEDGSLRGAAIDVFPEEPASINDP
                     FVSPLCKFDNVILTPHIGGSTSEAQANIGTEVANKFVKYSDNGSTVTAVNFPEVSLPL
                     HTNTKRLLHIHENRPGMLNQINQIFVESNVNIAAQYLQTDAAIGYVVIDVESDNVDEA
                     LQRLKAIEGTIRARVLY"
     gene            complement(2624..3283)
                     /gene="rpiA"
                     /locus_tag="PXE44_000713"
     CDS             complement(2624..3283)
                     /gene="rpiA"
                     /locus_tag="PXE44_000713"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL5588250.1"
                     /translation="MDQQEMKKIAAQAALKFVKPDTIVGVGSGSTVNCFIDALASMKD
                     EIKGAVAASKASEQRLRDIGIEVFSANEVTELDVYIDGADEITPQGAMIKGGGAALTR
                     EKIVSSLAKKFICIVDKSKQVDVLGSTFPLPVEVIPMARSYVARQLVALGGSPEYREG
                     VVTDNGNVILDVHNFKIMEPLKMEHTINNIAGVVTNGIFAQRFANVTIVGTPEGAVIL
                     E"
     gene            complement(3428..4762)
                     /gene="glmM"
                     /locus_tag="PXE44_000714"
     CDS             complement(3428..4762)
                     /gene="glmM"
                     /locus_tag="PXE44_000714"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL5588251.1"
                     /translation="MARKYFGTDGVRGEVGKFPITPEFALKLGWAAGKVLATQGTKKV
                     LIGKDTRISGYMLESALEAGLTAAGLSAVFVGLMPTPAIAYLTRTFRAEAGIVISASH
                     NPYYDNGIKFFSSVGEKLPDEIEEAIEAMLDKPMDCVDSANLGRASRINDAAGRYIEF
                     CKGTFPSELSLEGYKIVVDCANGATYHIAPNVMRELGAEVLEIGTKPDGLNINEKCGA
                     TDIKALQKVVVEAGADVGLAYDGDGDRLIMVDHLGNKVDGDQILFILAREALRSGKLQ
                     GGVVGTLMSNMSLELALKELAIPFSRANVGDRYVLEQLKEKGWKLGGENSGHIIVLDK
                     NTTGDGIIASLQVLAAMASHKLSLNDLAKAVPLFPQVLLNVRFEKGAANPLESNEVKA
                     VAADVEKRLAGKGRILLRKSGTEPLIRVMVECEDAVLAQSCAEEIVEAVKRN"
     gene            complement(4788..6320)
                     /gene="ilvA"
                     /locus_tag="PXE44_000715"
     CDS             complement(4788..6320)
                     /gene="ilvA"
                     /locus_tag="PXE44_000715"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDL5588252.1"
                     /translation="MMNETQHSQSAIDYLRSILSSNIYDLAQVTPLQKMEKLSERLGN
                     SIFIKREDRQPVHSFKLRGAFAMISNLSKAQKEAGVIAASAGNHAQGVALSAKHLGLR
                     ALIVMPQNTPAIKVDAVRGYGGEVLLYGANFDEAKAKAIELSTELGMTFIHPFDSPAV
                     IAGQGTIGLELVQQKPDLDYVFIPVGGGGLIAGIAVLIKQVMPNIKVIGVESKDSACL
                     YHALKQGQPTDLERVGLFADGIAVKRIGDETFRLCQQYVDDVVLVDNDEICASMKLIF
                     ENVRAISEPSGATSLAGLKKYVKEHQLQDKNLACILSGANVNFHTLRFVSERCEIGEK
                     REALLAVTIAEQKGSFLKFCEILGNRAVTEFNYRHSDDKQACIFVGVRISGSAEKVEI
                     IKDLQQNGYDVADLSDDDIAKTHIRYMVGGRASSIQYEQLYSFEFPEQKGALLKFLQM
                     LTNWDISLFHYRAHGADYGDILAAFALNRGDEVELNRHLEQLGYRFQNVSESPAYQYF
                     LK"
     gene            complement(6581..7174)
                     /gene="slyD"
                     /locus_tag="PXE44_000716"
     CDS             complement(6581..7174)
                     /gene="slyD"
                     /locus_tag="PXE44_000716"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL5588253.1"
                     /translation="MNITKDLVPSIAYQVRTQEGVLVDEAPVNQPLEYLHGHNNLVEG
                     LEKALEGKAVGDVFEVRVKPEEGYGEYNDNMVQRVPKDVFMGVDELEVGMRFIADTDL
                     GPLPVVITEVDGDEVVVDGNHMLAGQELLFSVEVVGIREATAEEIAHGHVHGGHEHGC
                     GCGGHDHHHDHDGCCGGHDHDHHHHHGHGGCGCGGHH"
     gene            complement(7277..7417)
                     /locus_tag="PXE44_000717"
     CDS             complement(7277..7417)
                     /locus_tag="PXE44_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588254.1"
                     /translation="MKKLIKVCGLISATIFLSGCLVTSVVGGVVGTAVDVVDTVTPDI
                     ID"
     gene            complement(7489..8175)
                     /gene="aqpZ"
                     /locus_tag="PXE44_000718"
     CDS             complement(7489..8175)
                     /gene="aqpZ"
                     /locus_tag="PXE44_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDL5588255.1"
                     /translation="MKKYFAEFFGTFWLVFGGCGSAVLAAGIPELGIGYAGVSLAFGL
                     TVLTMAYAVGHISGGHFNPAVSIGLLVGGRFNAKDLVPYIIAQVIGAIAAGAVLYTIA
                     SGVPTFDATAGFAGNGYGEHSPHGYSLTAALLIEVVLTAFFLIIIMGATDKRAPAGFA
                     PIAIGLALTLIHLISIPVTNTSVNPARSTGVALFQGSWAIEQLWLFWVAPIVGAIIGA
                     VVYRFIAEEK"
     gene            complement(8363..9082)
                     /locus_tag="PXE44_000719"
     CDS             complement(8363..9082)
                     /locus_tag="PXE44_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253715.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HDL5588256.1"
                     /translation="MKLKNFLTILPLAVATAVTSAETHTVEHKDKPASFNFSTEVSRT
                     VEKDVMQASVYSQKSGNSLPELKKFVSENLNRVLAEAKKHPSIEVQAEGVTNHVNYNN
                     KGNIDGWDTSGSIQLKSKDFDAMAAVLENLGKDVAIRYIDFSVSPEKMASLEDEMTLE
                     IISKFQHKADVIQKALGAKTYKLVNINIQTPNDGGRPYYEHRAMYAVSSMAQSKSMDE
                     IPLEAGKTTISATASGSVEFE"
     gene            complement(9140..9877)
                     /gene="tsaA"
                     /locus_tag="PXE44_000720"
     CDS             complement(9140..9877)
                     /gene="tsaA"
                     /locus_tag="PXE44_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDL5588257.1"
                     /translation="MNSTSNQLTPIQLNPIGFVESPYDEKFSVPRQPNLVAEGKGILR
                     LIPPYNTPDAVRGLEKFSHIWLIFQFHHIPEREWHATVRPPRLGGNERVGVFASRATH
                     RPNPLGLSKVKLEAVVAGGSEVYLKLGSVDLVNGTPIFDIKPYIAFADSEPQAVSGFA
                     QQKPQARLAVRFSEEAKQAVEFCRNFAKFGIEQPLSFLADVIAQDPRPAYQQGKPSER
                     VYGMNLAGYNIRWQIENDTQAIVLSIE"
     gene            10057..10965
                     /locus_tag="PXE44_000721"
     CDS             10057..10965
                     /locus_tag="PXE44_000721"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL5588258.1"
                     /translation="MNKNSLFSIWFTTARPKTLPLALASIIVGSALAHWAGKFDVVTT
                     LLAFITTILLQILSNFANDYGDHVKGSDTKERIGPLRAIQHGVIGGEQLKKAVIILSL
                     LSFVSGVALSAYAFETVQDLFFFIGLGIVSIIAAITYTVGKKAYGYLGLGDLFVLIFF
                     GFVAVIGVFYLQAHSLPIMILLPAFGCGLLSVAVLNINNLRDINQDRQAGKNTLIVRI
                     GSQNGRKYHVALLVLAVISYLIFAISEFEHWYSFIFLLSVPLLAKHARFVYQHKDPKE
                     LYPILGQMAGLALLTNLLFSLGIVLG"
     gene            complement(11041..12027)
                     /locus_tag="PXE44_000722"
     CDS             complement(11041..12027)
                     /locus_tag="PXE44_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL5588259.1"
                     /translation="MKKSLLALLIGSVFLISACEDGKMTQTVLEAEKQIVQLGTELKN
                     AQADLAKKEAEVAELSGVKAENEKLKSELENAQQNTALNVEIVKIFDKKEVIKHQVDP
                     KQEFAIEESTVSAFVSLPKTNFDWLNQLLINQAYDYNEEQGRKLKNPTEEEFKKHREA
                     VYQGLVESVKTEPAIGYDDHISSAFIGQRNNIATFTMMHYTYMGGAHGMHYTQYINVD
                     LAKKAIIGLNDLISPKNQVQLKDILWENYSSVRVDENGNYNGFADKKEFRISDQFYFT
                     PHGIAFVYPPYELGAYAEGDIEVEADWYIINKLLNSEYQRGEKDGFFEELDN"
     gene            complement(12121..13992)
                     /gene="rpoD"
                     /locus_tag="PXE44_000723"
     CDS             complement(12121..13992)
                     /gene="rpoD"
                     /locus_tag="PXE44_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL5588260.1"
                     /translation="MENQADQQLEQQSQLELLISQGREQGYLTLSEVHDHLPEELVDA
                     EQIEDIIQMINDMGIQVLEAAPDADDLLLAENIADEDVVEEATKVLSTVESELGRTTD
                     PVRMYMREMGTVELLTREGEIDIAKRIEEGINEVQCAIAEYPEALSGLLACYAQVETG
                     EMRLSDLVTAFVDPNILEEAPLEDIEAALGEDEESSDVPDVDDENEDENGDSSSDSSD
                     DSSIDPELAREKFEALRSQHEKVLLVIQKHGRTSKKSKEQIEILSHIFREFRLVPKQF
                     DLLVASMQKLMKQVRTEERQIQRYAVEYAGMKKADFLKAFQGHELSEAWIEKAINAKK
                     NAAPKLANYVDNIRVNIANLQNLEQTSGLTIAQIREIGGRISNGELKARRAKKEMVEA
                     NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
                     RSIADQARTIRIPVHMIETINKLNRISRQCLQEMGREATPEELAERMGMPEDKIRKVL
                     KIAKEPISMETPIGDDDDSHLGDFIQDESLELPLDSATAESLRMATNEVLEGLTPREA
                     KVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSETLRSFLDD"
     gene            complement(14251..15999)
                     /gene="dnaG"
                     /locus_tag="PXE44_000724"
     CDS             complement(14251..15999)
                     /gene="dnaG"
                     /locus_tag="PXE44_000724"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL5588261.1"
                     /translation="MKGTIPRSFIDDLIARTDIVEVINSRVKLKKAGRDYQACCPFHH
                     EKTPSFSVSQSKQFYHCFGCGVHGNVITFLMDYDKLEFPEAIEELAAMHGLEVPRENV
                     IQRDGKPQANFKTKRNLYELMEAVAKFYQQNLSHQIEPQSYLQQRGLSEEIIERFEIG
                     FAPNAFDAALRTFGQSQEDIQKLLDTGVLTKNDSGRIYDKFRNRVMLPIRDKRGRVIA
                     FGGRVLPNDEQGAKYMNSPETVIYHKGSELYGLYQALKVNETPDYLFVVEGYMDVVAL
                     AQFGVDNAVASLGTATTGEQIQQMFRCTEQIVCCYDGDRAGRDAAWRALENALPYLQD
                     GRQLKFIFLPDGEDPDTFVRSQGKEGFEQYVAQNAKPLSDFLFDSLLADVDLSSKEGK
                     SKLAALAVPLINRIPGEMLRVYLRNILGQKLGILDPTQIEALLPTKKAAEKPLHQAVK
                     MKRTPMRLLIALLIQNPYFVKFVPDVSALASLKDPGFTLLTDIVATCQQNAGISMGGL
                     LEHYREDPQYKIVEALANWDHLELPENIERTFIDTLDFLYAKLVELRKEELIAKDRTV
                     GLTDDEKKEYALLMFQ"
     gene            complement(16153..16368)
                     /gene="rpsU"
                     /locus_tag="PXE44_000725"
     CDS             complement(16153..16368)
                     /gene="rpsU"
                     /locus_tag="PXE44_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL5588262.1"
                     /translation="MPVIKVRENESFDVALRRFKRSCEKAGILAEVRAREFYEKPTTI
                     RKREKASLAKRHAKRNFRENARNTRLY"
     gene            complement(16516..17112)
                     /locus_tag="PXE44_000726"
     CDS             complement(16516..17112)
                     /locus_tag="PXE44_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HDL5588263.1"
                     /translation="MSLLPMNEITSLNQLDLQKSYSYADYLLWKFKERVEIIKGKIVA
                     MSPAPTRFHQRISMKMTAKFLEVFDNHQCQIYSAPFDVRFPDENGKVKTVVQPDLCVI
                     CDVSKLDERGCVGAPDLVVEILSPGNTKREMKDKYELYQEQGVKEYWIVSPETRTIQI
                     FVLENGKYIGIQPVAEDEPATSVVFPALSFSTEKLFEL"
     gene            17254..18510
                     /locus_tag="PXE44_000727"
     CDS             17254..18510
                     /locus_tag="PXE44_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL5588264.1"
                     /translation="MHLNFKKIDNSHIALFYNVLVATFFVTVLTFKKGYSYVPMALGI
                     IATLSFLFYRFKLKQKWLLDKEDKFFIFSLIGYFLTFAMSAIFNGDGIREIDNPIQIL
                     LLIPLIILFRLYPIKKETVLHFIPIGALVAGCIAIYQRFILNLKFPFPEIMRIQSGNI
                     AILLSLFSIAIAFYWLSKKRIKTGIAYLVFAMFGLFASILTGARGGWLAFIPICFFIL
                     FLNLRQLNKKAIFLILLALISSLSFFISKPEFGIQKRYNAAKNDIIVFLEKGNRHSSL
                     GARFDMWENALIGIREKPLLGHGSTGYETFKKKQIETKQMAKTTLRFNSLHNQYLEAF
                     VKRGVVGFMALMAILIIPFVIFAKRLKAKNREVKCMAILGIVHIISHSFFFLSQSFLA
                     HNSGSLFYFFVLILLYHLVKQNEEII"
     gene            complement(18612..19619)
                     /gene="rfbB"
                     /locus_tag="PXE44_000728"
     CDS             complement(18612..19619)
                     /gene="rfbB"
                     /locus_tag="PXE44_000728"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL5588265.1"
                     /translation="MIILITGGAGFIGSALVRYLIEHTPHTVINVDKLTYAGNLASLE
                     SVAHHERYHFEKADICASGQISEIFGKYQPDVVMHLAAESHVDRSISGAADFIQTNIV
                     GTYTLLEAARAYWDTLPEPRKSAFRFLHISTDEVYGDLAKSEELFTEKTPYNPSSPYS
                     ASKAASDHLARAWHRTYGLPTVITNCSNNYGAYQYPEKLIPLMILNALEGKSLPVYGN
                     GLQVRDWLFVEDHIRALYLVLQKGQIGETYNIGGHNEKTNIEVVRSICSILQEMVPNN
                     PLVVGQYADLITYVQDRPGHDFRYAIDTSKIEKELDWKPKETFESGLRKTVEWYLANR
                     N"
     gene            complement(19616..20197)
                     /locus_tag="PXE44_000729"
     CDS             complement(19616..20197)
                     /locus_tag="PXE44_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL5588266.1"
                     /translation="MLEKIEKLIDEINKLHLAFSKDYFETGKIEKVNLKHTFAKVPTP
                     HILAYRLNLHESINDYLYRADLEGISYFYRVKTSESILDKIERFKQRSEGYPVNSILN
                     DIFGARIIVSSEVIQQVMESLDYWQEKYGLKNWYLRDKDEYVGIHIYFKNSSNFYYPW
                     ELQIWDKKDAEKNIQSHIKYKRNFVNQISYSPL"
     gene            complement(20218..21489)
                     /locus_tag="PXE44_000730"
     CDS             complement(20218..21489)
                     /locus_tag="PXE44_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL5588267.1"
                     /translation="MSIQQKKRHSRWYERIFFSTAVQSILGVMFSSVLPAIYFWGEKF
                     NSIETRSNTIIATSLAFVMIAISLRVLMKYPGNKSASFVFSTVISWYAVLLIVLTIFR
                     FDYSNTLLGTSFILTILFCFIGYFLSRSWIIPKIALVPFGRAINLNRIQNVEWIQLSE
                     PILGEKRINSIAVDLHYPDLSAEWHKFLAKCTLNGIPVYNVRQLEESLTGRVKIRHMY
                     ENDLGSLLPSPIYSLVKRIIDIALIVATFPITLPIMTITAIAIRLESEGGAMFIQKRV
                     GQGGKEFNIYKFRSMCKDSEKDDAQFASSADMRVTRIGKFIRKTRIDELPQFFNVLKG
                     DMSLIGPRPEQTAFVDKFEEEIPFYNYRHIVKPGISGWAQVVQGYAADVDDTKVKVEH
                     DFYYIKHFSLWLDILIIFKTLKTMLTGFGAR"
     gene            complement(21493..22280)
                     /locus_tag="PXE44_000731"
                     /pseudo
     CDS             complement(21493..22280)
                     /locus_tag="PXE44_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         6509 a         4914 c         4051 g         6806 t
ORIGIN      
        1 taaatcagag gtttaaaaga tgaagatccc acaaatctta attgttgagg atgagttagt
       61 aacaaggaat atgctgaaaa gcatttttga aggagaagga tatcaggtat ttcaagcatc
      121 taacggcgag gaaatgcact ctgttctgag tacaaatgaa atcaatcttg tgctaatgga
      181 cattaactta ccgggaaaaa atggattaat tctcgcccga gaaattcgag agaatatgaa
      241 gctaccatta atgtttttaa ccggtcgcga caatgaaatt gataagatcc tcggtttaga
      301 aataggtgcc gatgactata tcactaaacc gtttaatcct cgagaattga ctatccgagc
      361 gagaaattta ttgcaacgaa cgatgataga taaagaaaac gacgttgtaa aagagcaatc
      421 tgaaattgaa agctatcgct ttaacggttg gatcttagac attaacagtc atagtttaat
      481 ctcaccagaa ggtgaggtaa ataaattacc acgtagtgaa tttagggctt tattacattt
      541 ttgccaacat cctggaaaaa ttcaaacacg tgaagattta ttgaaaaaaa tgaccgggcg
      601 ggaactaaaa cctcatgata gaaccgttga tgttactatt cgccgtattc gtaaacattt
      661 tgaagaccac cccaataccc cagaaattat tgtcaccatt cacggtgaag gctaccgttt
      721 ctgtggtaac atcgaacctt aatcaagata ggagtaattt tatgaaaaaa ttaactattg
      781 tcgcattaac ttgcttattt tctacccatc tattagccgc tcctgtcgga aatacattta
      841 ctggcatcgg tgtcggtgta gatgtaacta caacaaaata caaagttgaa ggagtaaaag
      901 gcaaggattc cacaggtccg gcattagttg tcgattatgg tattgagcaa ggcaataact
      961 ttgtaggcgt tgtacaagga aaagcaaaag taggtagtac aaaagtattt agcgatgtaa
     1021 aacaaaaaaa taaatatact atagcttatc aacaaggtta tcgtgttgga tccgatttat
     1081 taccttatgt aaaagtagat gccagctcaa gcaaaatcgg taatgaaacc tatcgtggct
     1141 ttggtttcgg tgctggtgca aaatatgcag tttcaagcga tgttgaatta ggtgcagaat
     1201 acactcgcga taatttaaaa cgtagcggca caaaattaaa aggtaacgct ttcaccgcga
     1261 atgcaactta tcgtttctaa tcttagcggt aaagttttct gaaaaaataa ccgcttgtaa
     1321 cacaaaagag cctgaagtga ttcaggctct ttgcctttta atataatact cgggctcgaa
     1381 tcgtgccttc gatcgctttt aaacgttgta atgcttcatc aacattgtcg ctctctacat
     1441 caattacaac ataaccgata gctgcatcgg tttgtaaata ttgagcggca atattcacat
     1501 ttgattccac aaaaatctgg ttaatttgat ttaacatacc tgggcgattc tcgtgaatat
     1561 gtaataaacg tttggtatta gtatgaagcg gtaaggatac ttcagggaaa ttcactgccg
     1621 tcacggttga accgttatct gaatatttca caaatttatt ggcaacttcg gtgccgatat
     1681 ttgcctgagc ttcagaggtt gaaccaccaa tatgtggcgt taaaatcacg ttatcaaact
     1741 tgcacaatgg tgacacgaac gggtcattaa ttgatgccgg ttcttccgga aatacgtcaa
     1801 tcgccgcacc acgcaatgag ccgtcttcaa tccgagcggc taatgcgtct aaatcgacca
     1861 ctgtaccacg agcggcatta attaacaccg agccttcttt taattgagca atgcgttctg
     1921 cactcattag gtttttagtc gagtcgattt ccggaacgtg tagtgaaatc atatcgcagg
     1981 ttgaaagcaa atgctctaat gtcggcactt gctgtgcatt accgagtggt agtttgtttt
     2041 caacatcata gaaataaacc tgcataccaa tggcttcggc aagaatacta agctgtgaac
     2101 cgatatgacc atatcccaca atacctaatt tcttgccacg tacttcgttt gcacctgctg
     2161 ccgatttatt ccacacccca cgatgcactt ccgcatttgc agtcgggact ttacgcatta
     2221 ataaaatcat ctctgccagc actaattctg ccaccgagcg ggtatttgag aacggtgcat
     2281 tgaataccgg aatacctcgt cttttcgccg cattcaaatc aacttggttt gtaccgatac
     2341 agaaacaacc aatcgcaatt aatttcggag cttgagccag tacttcttcg gttaaaaaag
     2401 tacgcgagcg aataccaaca aagtgtgcat ctttaattgc atcaattaat tcttgaccgt
     2461 ctaatgcttt tttatgcact tcaatattgg tataaccagc cgctaagagt acatcaattg
     2521 cgttttgatg tacgccctca agcagaagga atttgatctt ggatttatcc aaagaaattt
     2581 tagccatttt ttgctcctaa gtatttgtat ttgcaataat aaattattct aaaataactg
     2641 caccttcagg tgtgccgaca atggttacat tggcaaaacg ttgtgcaaaa ataccgttag
     2701 tcaccacgcc tgcaatatta ttaatggtat gttccatttt caacggctcc ataattttaa
     2761 agttatgtac atctaaaatc acattgccgt tatcagtgac cacgccctca cggtattccg
     2821 gcgaaccacc taaggctacg agctgacgag ccacataaga acgagccatt gggatcacct
     2881 ccaccggcag agggaaagtt gaacccagca catcaacttg tttggattta tccacaatgc
     2941 agataaattt tttcgctaat gaactcacga ttttctcacg ggtaagtgct gccccgccac
     3001 ctttaatcat cgccccttgt ggcgtgattt catcagctcc gtcaatatac acatctaatt
     3061 ccgtcacttc attggcactg aatacttcaa tgccgatatc acgcaaacgc tgttccgagg
     3121 ctttagaggc agcaaccgct cctttaatct catctttcat tgaagcaagg gcatcaataa
     3181 aacagtttac ggttgaacca cttcccaccc caacaatagt gtccggtttt acaaatttta
     3241 atgcagcttg tgccgcaatt tttttcattt cttgttgatc catttttacc tccacttgta
     3301 gggcaaatca tcatttgccc acttcggcaa ggcgaaaaaa attttcgccc tactacatct
     3361 attctacaca tatatgtaac aagcggtcat atttggcaaa ttttttacca aaaacgaccg
     3421 cttgtcatta gttacgtttt actgcttcaa caatctcttc cgcacagctt tgagcaagta
     3481 cagcatcttc gcactccacc atcactcgaa ttaatggctc agtgcctgat ttacgcaata
     3541 aaatacgccc tttgcctgcc aagcgttttt ccacatctgc cgctaccgct ttcacttcgt
     3601 tactttcaag cggatttgcc gcccctttct caaagcgaac atttagcaga acttgcggga
     3661 acagaggcac tgcttttgcc aaatcattca ggcttaattt gtggcttgcc attgctgcta
     3721 atacttgcag tgaggcaata ataccatcac cggtggtgtt tttatctaac acgataatat
     3781 gcccggagtt ttcgccaccc agtttccagc ctttttcttt aagctgctct agtacatagc
     3841 ggtcacccac gttagcacga gagaatggaa tggctagctc ttttaaagct aattccaagc
     3901 tcatattact cattaatgta ccgactacac cgccttgtaa tttgcctgaa cgtagagctt
     3961 ctcgggctaa aataaataga atttggtcgc catcaacttt attgcctaag tgatcgacca
     4021 taatcaaacg gtcgccatca ccatcgtaag ctagaccaac atccgcacct gcttctacca
     4081 ccactttttg aagtgcttta atatccgttg caccacattt ttcgttgatg tttaaaccgt
     4141 ccggcttagt accgatttca agcacttctg cccctagctc acgcattaca ttcggcgcga
     4201 tatggtaggt tgcaccgttg gcacaatcca ccacaatttt atagccttca aggcttagtt
     4261 cagacgggaa agtgccttta caaaactcga tataacgtcc tgcggcatca ttaatacgac
     4321 tggcacgacc taaatttgct gaatctacgc aatccatagg cttatcaagc attgcttcaa
     4381 tcgcttcttc aatttcatca ggtagttttt caccaaccga cgagaagaat ttaatgccgt
     4441 tatcgtaata aggattgtga gaggcagaaa tcacaatgcc ggcttccgca cggaaagtac
     4501 gggttaaata agcaattgcc ggagtcggca tcagaccaac gaatactgct gataaaccgg
     4561 ctgcggttaa accggcttcc agagccgatt ccaacatata accggaaata cgggtatctt
     4621 taccgattaa tacttttttc gtgccttggg ttgccaacac tttacctgct gcccaaccga
     4681 gttttaatgc aaattcaggt gtaattggaa atttacctac ttcaccacgc acaccgtctg
     4741 tgccgaaata tttacgagcc attctaattc ctaagattga taaaaattta ctttaaaaaa
     4801 tattgatatg ccggactttc gctcacattt tggaagcggt aacctagctg ttctaaatgt
     4861 cggtttaatt ctacttcatc gcctctgttt aatgcaaatg cggcaagaat atcgccataa
     4921 tcggctccgt gagctcggta atggaataac gagatatccc agttagtcag catttgtaag
     4981 aattttagca atgccccttt ctgctctgga aattcaaagc tatagagttg ctcatattga
     5041 atggagcttg ctctaccgcc taccatataa cgaatatggg ttttggcaat atcatcgtcc
     5101 gataaatctg ccacatcata gccgttttgt tgcagatctt taataatctc tactttttct
     5161 gccgagcctg aaattcgcac ccccacaaaa atacaggctt gtttatcatc ggaatggcga
     5221 tagttaaatt ccgttaccgc acggttgcct aaaatctcgc agaattttaa gaaactacct
     5281 ttctgttcag caatggtgac tgccaataaa gcttcacgtt tctccccgat ttcacaacgc
     5341 tctgacacaa aacgtaaggt gtggaaattc acattcgccc cggaaagaat gcaagctaag
     5401 tttttatctt gtaattgatg ctctttcaca tattttttca aaccggcaag ggaagttgcc
     5461 ccggacggct cggaaatcgc acgcacattt tcaaaaatca acttcattga ggcacagatt
     5521 tcatcattat ccaccaacac cacatcatca acatattgct gacacaggcg gaaagtttca
     5581 tcgccaatgc gttttaccgc aataccgtca gcaaaaagcc caacccgttc taaatcggtc
     5641 ggttgccctt gctttaaggc gtgatataag caagcagaat ctttagattc gaccccaatc
     5701 actttaatat tcggcatcac ctgcttaatt aaaaccgcaa tgccggcaat taagccgcca
     5761 ccgcccactg gaataaatac ataatctaaa tccggctttt gttgcaccag ttctaagccg
     5821 attgtgcctt gccctgcaat cactgccggg ctgtcaaacg ggtgaatgaa ggtcataccc
     5881 aattcggtgg aaagctcaat cgctttcgcc ttagcttcat caaaattcgc cccataaagc
     5941 aacacttcac caccatagcc acgtaccgca tcaaccttaa ttgccggtgt gttttgcggc
     6001 attacaatca aggctcttaa acctaaatgt ttggcagaaa gtgccacccc ttgtgcgtgg
     6061 ttgcctgctg aagccgcaat gactccagcc tctttttgtg ctttggataa atttgagatc
     6121 atcgcaaaag cacctcgcaa tttaaagctg tgaaccggct gtcggtcttc acgtttgata
     6181 aaaatagagt tgcctaaccg ctcagacagt ttttccattt tctgtaaagg ggtcacttgc
     6241 gctaagtcat aaatatttga gcttaaaatc gagcgtaaat agtcgatagc ggattgggaa
     6301 tgttgtgttt cgttcatcat tttgaaaggt tatttattgt tagattgaga taaagacatt
     6361 ggtaaatttc caaggaaaat tgaccgcttg tttggttatt tagcaatggc tttaacaggg
     6421 aatatttcct ctcgcccgtt tgcgggagag agacagctta aaatttcgtt cagaaatttt
     6481 aagcagagag aggggaaacc ttggatatcc ccctctccct ccgcaaatct tcgatttgct
     6541 ccaccctctc ccgcaagcgg ggagagggga aatcacaaag ttagtgatga ccaccgcaac
     6601 cacagccacc gtgtccgtga tgatggtgat gatcgtggtc gtgaccgccg cagcaaccat
     6661 cgtgatcgtg atgatgatcg tgcccaccac aaccgcaacc gtgttcgtgt ccgccgtgaa
     6721 cgtgtccgtg tgcgatttct tccgctgtcg cttcacgaat gccaaccact tccacagaga
     6781 aaagtaattc ctgaccggct aacatatggt taccgtccac cacaacttca tcgccatcta
     6841 cttcggtaat taccaccggt agcggaccta aatccgtgtc cgcaataaaa cgcataccca
     6901 cttctaattc atcgacgccc ataaatacat ctttcggtac acgttgaacc atattatcgt
     6961 tgtactcgcc gtaaccttct tccggtttca cacgcacttc aaacacatcg cctacagctt
     7021 tgccttcaag ggctttttct aagccttcta ccaagttgtt gtgaccgtgt aaatattcta
     7081 acggttggtt taccggtgct tcatctacta atacgccttc ttgagtacgc acttgatatg
     7141 caatgcttgg cactaaatct tttgtaatgt tcatattgaa tcctttaatt catcggtttt
     7201 ataaaatagc aagccattgt aacgctttat tttatatttg caatctatct ggaaatttta
     7261 accgcttgtc agtaaattag tcgataatat caggggttac ggtgtctacc acatcaactg
     7321 cggttcccac cactccacca accaccgaag ttaccaaaca accacttagg aaaatagttg
     7381 ctgaaatcag cccacatact ttgattaatt ttttcatttt aaaatccttt caaaaacaaa
     7441 aaacagccct aatcaagact agagctgcat catatttatt cgcttaaatt atttttcttc
     7501 agcgataaag cgataaacta ccgcaccgat aattgcaccg acaatcggag caacccagaa
     7561 caaccataat tgctcaatcg cccagctgcc ttggaataac gctacaccgg ttgaacgtgc
     7621 cgggtttact gaggtattgg ttaccggaat gctgattaag tggattaagg ttaaagccaa
     7681 accaatagca atcggagcga aacctgctgg agcacgttta tcggttgcac ccataatgat
     7741 gattaagaag aatgccgtta ataccacttc aattaataat gctgctgtta agctatagcc
     7801 gtgtggcgaa tgctcaccat aaccgttgcc ggcaaagcct gctgtagcat caaaagtcgg
     7861 aacacctgaa gcaatggtgt ataacactgc accggcagca atcgcaccga ttacttgggc
     7921 aatgatataa ggcactaaat ctttagcgtt aaaacgaccg ccgactaata aaccgattga
     7981 aaccgccggg ttgaagtgac cacctgaaat atgaccgact gcatacgcca ttgttaatac
     8041 ggttaaaccg aaggctaacg aaacgcctgc ataaccgatt cctaattcag gaatgcctgc
     8101 tgctaaaact gcactgccgc aaccaccaaa cactaaccaa aatgtcccga aaaactcagc
     8161 aaaatatttt ttcatttttt atccttaccc taaagggtga atcacacata agtgtattaa
     8221 ctttccgaat gctataaaac atagccaata aaatatgagt atactaaaaa aagtaaagac
     8281 gcatatttct taattgatag tatagaccga ttttttcctc caattaaagt aaaaatacaa
     8341 ctctatacaa atctatacat ttctattcaa attccacact tcctgaagcg gtagctgaaa
     8401 ttgtggtttt acctgcttct aacgggattt catccataga tttgctttgt gccattgatg
     8461 aaactgcata cattgctctg tgttcataat atggtctacc accatcattt ggagtttgaa
     8521 tattgatatt aactagctta tatgttttcg cccctaaggc tttttgaatc acatcggctt
     8581 tatgctgaaa tttgctgata atttccagcg tcatttcatc ttcaagggac gccattttct
     8641 ctggcgatac actaaaatca atgtaacgaa tcgccacatc tttacctaaa ttttccaaca
     8701 ccgctgccat tgcatcaaaa tctttgcttt tcaactgaat gctaccagag gtatcccaac
     8761 catcaatatt ccctttgttg ttataattaa cgtgattagt cacaccttca gcttgtactt
     8821 caatcgatgg atgttttttt gcctcagcca atacacgatt caggttttca gatacaaatt
     8881 ttttcagctc cggcaaggag ttacctgatt tttggctata cacacttgcc tgcatcacgt
     8941 ccttttccac cgtgcgagag acttcggttg agaaattaaa agaagccggt ttatctttat
     9001 gttccacggt atgagtttcc gctgaagtta ccgctgttgc gactgcaagc ggtagaatgg
     9061 taagaaaatt tttcaatttc ataatcagtc cttaaaaggg ttaaaaatca tttcttagat
     9121 ctcacttgcg taggaaagtt cattcaatcg aaagcacaat cgcctgtgta tcattttcaa
     9181 tttgccagcg aatattgtag ccggcaaggt tcataccata aactcgctca ctaggtttcc
     9241 cttgttgata cgccgggcga gggtcttggg cgatgacatc ggcaagaaaa ctaagcggct
     9301 gttcgatgcc aaattttgca aaatttcggc aaaattcgac agcttgtttt gcctcctcgc
     9361 taaaacgcac cgctaatctt gcttgtggtt tttgctgagc aaaaccggaa accgcttgcg
     9421 gctcgctatc tgcaaatgca atgtaaggct tgatgtcaaa aattggtgtg ccgttaacta
     9481 aatccacgct gcctaatttc aaataaactt cactcccccc cgccactacc gcttctaact
     9541 tcactttcga taagccaagc ggattcggac gatgggtggc acggctggca aagaccccga
     9601 cccgttcgtt cccgcccaag cgaggcggtc tgacggtggc gtgccactct ctttcgggaa
     9661 tgtgatggaa ttggaaaatc aaccaaatat gactaaattt ctccaagcct ctgactgcat
     9721 cgggggtatt gtaaggcgga attaaacgta gaatgccttt cccctccgct accaaattcg
     9781 gctggcgagg cacggaaaat ttttcatcat aggggctttc cacaaaaccg atagggttta
     9841 attgaatcgg ggttagttga tttgaggtag agttcattac gcttttttct gttaaaataa
     9901 tataaaatat tggacataat gatatgaaat tgaggatggt gcaagcgtcc acgcttgtac
     9961 caattcgttt taccatttaa cacaagagtg gacgcttgca ctatcgctta aatccttaac
    10021 atcattgtaa atgaaattac gctaagatta ttctctatga acaaaaattc tctcttttcg
    10081 atttggttta ccaccgctcg cccgaaaact ttaccgctgg cgttagcttc aattattgtg
    10141 ggttcagcac ttgctcattg ggcagggaaa tttgatgttg ttaccacctt actcgcattt
    10201 atcaccacca ttttgttgca gatcctgtct aacttcgcca atgattacgg cgaccacgtc
    10261 aaaggctctg acaccaaaga acgaatcgga ccgctacggg cgattcaaca cggtgttatt
    10321 ggcggtgaac aacttaagaa agccgtgatt atcttaagcc tactctcgtt tgtttccggt
    10381 gttgccttaa gtgcctatgc ttttgaaaca gtgcaggatc tgtttttctt tatcgggctt
    10441 ggcatcgtct caattattgc cgccattact tacaccgtcg gcaaaaaagc ctacggctat
    10501 ttggggctgg gcgatctatt tgtgctgatt ttctttggct ttgtagcggt aatcggcgtg
    10561 ttctacttgc aggctcatag cctacctata atgattctgc tccctgcttt cggttgcgga
    10621 ttgctgtctg tagcagtgtt aaatatcaat aacttgcgtg atattaacca agaccgacaa
    10681 gctgggaaaa acactctcat tgtacgaatc ggtagccaaa acgggcgaaa ataccacgtt
    10741 gcattgttag tgctagcggt catttcttat ctgatttttg caatttctga atttgaacat
    10801 tggtatagct tcattttttt gctgtctgta ccgttactgg caaaacacgc tcgttttgtc
    10861 tatcagcata aagacccaaa agaactctac ccaattctag ggcaaatggc agggctggcg
    10921 ttgctcacta atctattatt tagtttgggc attgtgttag ggtaagttgc aaaattttaa
    10981 caaaactaaa ccgcttgtaa taaaggatta caagcggttg ttttttatct aaatttacaa
    11041 ttagttatct agttcttcaa agaatccatc tttttcgcca cgttgatact ccgaattgag
    11101 caacttatta ataatgtacc aatccgcttc aacctctata tcgccttctg cataagcacc
    11161 taattcgtat ggcggataaa caaaagcaat gccgtgaggg gtaaagtaga attggtcaga
    11221 aatacggaac tcttttttat cagcaaatcc attatagttg ccattttcat ccacacgcac
    11281 gctactgtaa ttttcccaaa gaatgtcttt cagttgaact tgattttttg ggctgattaa
    11341 atcatttaag ccaatgattg cttttttagc taaatctaca ttgatatatt gcgtatagtg
    11401 cataccgtga gccccaccca tataggtgta atgcatcata gtgaatgtag caatattatt
    11461 gcgttgcccg ataaaagcac tggaaatatg atcatcatag ccaatagctg gctcggtttt
    11521 cacactttct accaaccctt gataaaccgc ttcacgatgc tttttaaact cttcttcggt
    11581 tggatttttc agctttctgc cttgttcttc attgtaatca taggcctgat taatcaaaag
    11641 ctggttaagc caatcaaaat tggtttttgg tagagacaca aacgcactta ctgtactttc
    11701 ttctattgca aattcttgtt tcggatcaac ttggtgctta atcacctctt tcttatcaaa
    11761 gattttcaca atttcaacat ttaacgctgt gttttgctga gcgttctcta actctgattt
    11821 taatttttca ttttctgctt tcacccctga aagttctgca acttcagcct ctttttttgc
    11881 taaatcagcc tgagcatttt ttaattctgt gcctaactga acaatttgtt tttcggcttc
    11941 taataccgtt tgggtcattt tgccgtcttc acaagctgaa atcagaaata ctgagccaat
    12001 taaaagtgct aatagtgatt ttttcatctc tccctctttt gtaaaaaata cggtaaatat
    12061 caccgcttgt tatacagatt aaaacaaaat cccctatacc atcaggcata ggggaaatga
    12121 ttagtcgtct aagaaactgc gtaaggtttc cgaacggctc gggtggcgaa gtttgcgcag
    12181 tgctttcgcc tcaatttggc ggatacgttc acgagttacg tcaaattgtt taccaacttc
    12241 ttcgagtgtg tggtctgtgt tcatatcaat accaaaacgc atacgcaaca ctttcgcttc
    12301 acgcggggtt aagccctcta acacttcgtt cgttgccata cgcaggctct ccgcagtggc
    12361 ggaatccaat ggtaattcaa ggctttcgtc ttgaatgaaa tcacctaaat gtgaatcgtc
    12421 atcgtcaccg attggtgttt ccattgaaat cggctctttg gcaattttca atactttgcg
    12481 gattttatct tccggcattc ccatacgttc tgccagttct tccggcgttg cctcacgccc
    12541 catttcttgt aaacattggc gtgaaatacg gttgagtttg ttgatagttt caatcatatg
    12601 caccggaata cgaattgtac gggcttggtc agcaatcgaa cgggtaatcg cttgacgaat
    12661 ccaccaagtt gcataagtcg agaatttata accacggcgg tattcaaatt tatccaccgc
    12721 tttcattaaa ccgatgtttc cttcttggat taaatctaag aactgcaagc cacggttagt
    12781 gtattttttc gcaatcgaaa tcaccaaacg taagttagct tccaccatct cttttttcgc
    12841 acggcgggct tttaactcac cattggaaat acgcccgcca atttcacgaa tttgagcaat
    12901 ggttaagcct gatgtttgct ctaaattctg taagttagca atattgacac ggatattatc
    12961 cacataatta gctaatttcg gtgctgcatt tttcttggca ttaatcgctt tttcaatcca
    13021 cgcttcgctt aattcgtgcc cttggaatgc tttcaagaaa tccgcttttt tcatgccggc
    13081 atattccact gcatagcgtt gaatttgacg ttcttcagtg cgaacctgct tcatcagttt
    13141 ttgcatagaa gcaaccaata agtcaaattg cttcggcacc aaacggaatt cacggaaaat
    13201 atgagataaa atttcaattt gctctttcga ttttttactg gttcgcccgt gtttttgaat
    13261 gaccaataac actttttcat gttggctgcg tagggcttca aatttttcac gggcaagttc
    13321 cgggtcaatt gagctgtcat cactgctgtc tgaactgcta tcaccgtttt catcttcgtt
    13381 ttcgtcatct acatcaggca catcagaaga ttcttcatct tcacctaaag cagcctcaat
    13441 atcctctaac ggtgcttctt ctaaaatatt aggatcgaca aatgctgtca ctaaatcaga
    13501 aaggcgcatt tcaccggttt cgacttgagc atagcaggct aacaggccac ttaaggcttc
    13561 cggatattcc gcaatcgcac attgaacttc gttaatccct tcttcaatgc gtttagcgat
    13621 atcaatctca ccttcacggg ttaaaagctc aactgtcccc atctcacgca tatacatacg
    13681 cacagggtca gtagttctac ctaactctga ttctacggtt gaaagcactt tggtggcttc
    13741 ttctaccaca tcttcatcgg cgatattttc agcgagtagt aaatcatccg catcaggggc
    13801 agcctctaaa acttggatac ccatatcgtt gatcatctga ataatatctt cgatctgctc
    13861 cgcatccacc aactcttccg gtaagtggtc gtgcacttca gaaagcgtta aatatccctg
    13921 ttcacgccct tgactgatca gaagttctaa ttgagattgc tgttctaatt gttgatctgc
    13981 ttggttttcc atagtctatc cgtttattat gcgagcaaaa atagtagtgg attataccac
    14041 taaatttggt tcgacttcat aaattcacaa aaaaattcta tttttctaat ttacacatta
    14101 ctttgatgct tatgagaaca gagaggtgta gcaagagaga taaataactt tctttttaga
    14161 tagaattaat tattttcttc gttcatcact tctgccaaat ctcccctaac ccctctttgc
    14221 taaagagggg aattattcga gcttaaaatt ctactgaaac atcaaaagag catactcttt
    14281 tttctcatca tcggttaagc ccacggttcg gtctttggca attagctctt ctttgcgtaa
    14341 ttccactaat ttggcgtaga gaaaatcgag agtgtcaata aatgttcgtt cgatattttc
    14401 gggtaactct aaatgatccc aatttgcgag ggcttcaaca atcttatatt ggggatcttc
    14461 ccgataatgt tcaagtagcc cgcccatact gatacctgca ttttgctgac aagttgcgac
    14521 aatatcggtt aataatgtaa agccaggatc tttcaaggag gctaaagcac ttacatcggg
    14581 tacgaatttc acaaaatacg gattttgaat caataacgca atcaacagcc gcattggcgt
    14641 acgtttcatt ttgaccgctt gatgtaaagg cttctctgcc gcttttttcg tcggtaacag
    14701 agcttctatc tgcgttgggt ctaaaatgcc taatttctgt cctaaaatat tgcgtaaata
    14761 cactcgcagc atctcgcccg gaatacgatt aattaacggc acagctaaag cagcgagctt
    14821 ggattttccc tcttttgaag agagatccac atccgccagc aaactgtcaa ataagaaatc
    14881 gcttaatggc ttggcattct gtgccacgta ctgctcaaat ccttctttac cttgagaacg
    14941 aacaaaggtg tccgggtctt cgccatcggg caagaaaata aatttcagtt ggcgaccgtc
    15001 ttgcaaataa ggcaacgcat tttctaacgc ccgccaagct gcatctcgac cggctctatc
    15061 gccatcataa caacagacaa tttgctcggt gcaacgaaac atttgctgaa tttgttcacc
    15121 ggtagttgcc gtgcctaaag aggcgactgc attgtccacg ccgaattggg caagggcgac
    15181 cacgtccata tagccttcca ccacgaacag ataatccggc gtttcattga ctttcagtgc
    15241 ttgatacaag ccatataact cactgccttt atgataaatc accgtttcgg gcgagttcat
    15301 atatttcgcc ccttgctcat cattcggcaa cacccgccca ccaaaggcaa ttacccgtcc
    15361 acgcttatct cgaatcggta acattacccg attgcgaaat ttatcgtaaa ttcgaccgct
    15421 atcatttttg gttaaaacgc ccgtatcgag caatttctga atatcttctt gtgattgccc
    15481 aaaagtgcgt agggctgcat caaaggcatt cggagcaaaa ccaatttcaa agcgttcgat
    15541 aatctcttca gacaaacctc gttgctgtaa atagctttgt ggctcaatct ggtgcgataa
    15601 attctgctgg tagaacttcg ccaccgcttc catcaattca taaagattac gcttagtttt
    15661 aaaattagct tgaggcttgc cgtcacgttg aatcacattc tctctcggta cttccagccc
    15721 gtgcatagct gccagttctt caatcgcttc cggaaattcg agtttgtcat aatccatcaa
    15781 aaaggtgatg acattgccgt gtacgccaca gccgaaacaa tggtaaaact gttttgattg
    15841 gcttaccgaa aaggaagggg ttttctcgtg atgaaaagga cagcaggctt gataatctcg
    15901 cccagccttt ttgagtttga cccgactgtt gatgacctca acaatatcgg tgcgagcgat
    15961 aagatcatca ataaatgaac gtggaattgt gcctttcatt gatttcctcc tgctctttga
    16021 atgatacaag cggagcaatt ttgcaaattt tttgcaaaaa acaaccgctt gaaatagaaa
    16081 cgttaaaaac accaaaaccg tgagcctaac ggaatcacgg tttcgatata gtttgctaac
    16141 cagcctaact aattagtata aacgtgtgtt acgtgcgttc tcacggaaat tacgtttagc
    16201 gtgacgttta gcaagtgatg ctttttcacg tttacgaatt gtagttggtt tttcgtagaa
    16261 ttcacgagca cgaacttcag ctaagatacc cgctttttcg caagagcgtt tgaaacgacg
    16321 taatgctacg tcaaatgatt cattttcacg aactttaatt actggcatat gccatcacct
    16381 cagaaaaata tgattaaatt aaaccgcaca caattttggc ggcatagaac aaaaaaggta
    16441 gtgcattcta ccttaagcac tcacataagt aaagtagtat tgtggcataa aaccgccgta
    16501 ttatgttgca tttttctaca gttcgaataa tttctcggtc gaaaaactta atgccggaaa
    16561 gaccaccgaa gttgccggtt catcttctgc caccggctga ataccgatat atttaccgtt
    16621 ttccaacaca aaaatctgaa tagtgcgggt ttcgggagaa acaatccaat actctttcac
    16681 cccttgttcc tggtaaagct cgtatttatc tttcatctcc cttttggtat tacccggaga
    16741 aaggatttcc accactaaat ccggtgcccc tacacagcct ctttcatcta acttactgac
    16801 atcacaaatt acacaaagat caggctgcac tacagttttg acttttccgt tttcatcggg
    16861 aaaccgaacg tcaaaaggcg cagaataaat ctggcattgg tgattgtcaa aaacctctaa
    16921 aaattttgct gtcatcttca tagaaattcg ctgatgaaaa cgagtaggag ccggagacat
    16981 cgccacaatt ttccctttga taatctcaac ccgctcttta aatttccata gcaaataatc
    17041 agcgtaactg taactttttt gtaagtccag ttgatttaag ctggtaattt cgttcattgg
    17101 taataagctc ataccgcctc ctttatcaga aatataaaga tattatgccg aataatccct
    17161 caaattcaac aaaactttcg ctaaaaagag atagataacc tttataccct tagtataaaa
    17221 tgacagcaat tttcattgtt ttaggatatg tcgatgcacc ttaacttcaa aaaaatagac
    17281 aactcacata ttgctctttt ctataatgta ctagtcgcca cattttttgt cactgtttta
    17341 acctttaaga aaggttatag ttatgtgcca atggcattag gtattatcgc tacacttagt
    17401 ttcttatttt atcgtttcaa attaaaacaa aagtggttac ttgataaaga ggataaattt
    17461 tttattttct cgcttattgg ctattttcta acgtttgcaa tgtctgctat ttttaatggc
    17521 gatggcattc gtgaaattga taatcctatc cagattttat tgcttatccc tttaatcatc
    17581 ctctttagac tttacccgat taaaaaagaa actgtattgc actttatacc gattggagcg
    17641 ttggtagctg gttgtatagc aatatatcag aggtttatat taaatcttaa atttcctttc
    17701 cctgaaataa tgcgtattca atcggggaac atagcaatat tattatcact atttagtata
    17761 gcaattgcct tttattggct ttcaaaaaaa agaattaaaa ctggcattgc ctacctggtt
    17821 tttgccatgt ttgggttatt tgccagtatt ctaacaggag caagaggcgg ctggcttgca
    17881 ttcattccca tttgcttttt tatcctattt ttaaatttaa gacagctaaa taagaaagct
    17941 attttcctga ttctattagc acttatatct tcactctcat tttttatctc caaaccagaa
    18001 tttggcatac aaaaacgata taatgcagct aagaatgata ttatcgtatt tttagaaaaa
    18061 ggaaatagac attcatctct gggagctcgt tttgatatgt gggaaaatgc gcttatcgga
    18121 atccgtgaaa aaccgctatt aggacacggt agcacaggct atgaaacatt caaaaagaaa
    18181 cagattgaaa caaaacaaat ggcaaaaaca acgcttaggt ttaatagctt acacaaccaa
    18241 tacctagaag cctttgtgaa aagaggtgta gttggtttta tggcgctaat ggctatttta
    18301 attattccat ttgttatttt tgccaaacga ttaaaggcaa aaaatagaga ggtaaaatgt
    18361 atggcgatat taggcattgt acatattatt tcgcattcat tctttttcct aagccaatca
    18421 tttttagccc ataattcagg ctcattattc tatttctttg tgttaatttt actctaccat
    18481 ttagtcaaac agaacgaaga gataatatga taaaacaata gaagaaagtt gggaatataa
    18541 ccgaggtggt aacttcatct tctaccacag gataaatgtc gggatatttc ctctttttct
    18601 gcttcttact actaattccg attagccaaa taccattcca ctgttttacg caagccactt
    18661 tcaaaagttt ctttcggttt ccagtcgagt tccttttcga tttttgaagt atcaattgca
    18721 tagcggaaat cgtggccagg gcgatcttgc acataagtaa ttaaatcagc atattgtcct
    18781 acaacaagcg ggttatttgg caccatttct tgcaaaatag aacagataga acgaaccact
    18841 tcgatattgg ttttttcatt atgcccgcca atattgtagg tttctccgat ttgccctttt
    18901 tgcaacacca aatagagggc acgaatatga tcttccacaa acagccaatc tctgacctgc
    18961 aagccgttgc cgtaaacagg tagggattta ccttctaagg catttaaaat cattaacgga
    19021 ataagttttt cgggatattg gtatgcccca taattattgg aacagttggt gatgacagtc
    19081 ggtaaaccgt aggtgcgatg ccaagcccga gccagatgat cgctggcagc tttagatgcc
    19141 gaataggggc tacttggatt ataaggggtt ttctcggtaa ataattcctc gcttttggct
    19201 aaatcaccat agacctcgtc ggtcgagata tgtagaaagc gaaacgccga tttccttggc
    19261 tcaggtaacg tgtcccaata ggctctcgct gcctctaata aagtataagt gccgacaata
    19321 ttggtttgaa taaaatctgc tgccccagaa atagagcgat caacatggct ttccgctgct
    19381 aagtgcatta ccacatcagg ctgatatttg ccaaaaatct cagaaatttg accgcttgca
    19441 caaatatctg ctttttcaaa atggtagcgt tcgtgatgtg cgacactctc caatgaggct
    19501 aaatttcccg cataggtgag cttatccaca ttgataactg tgtgtggagt atgttcaatc
    19561 agatagcgaa ctaatgcaga tccgataaaa ccggctccgc cagtgatgag gatgatcata
    19621 aaggtgaata tgaaatttga ttcacgaaat ttcgtttgta tttaatatgg ctttgaatat
    19681 tcttttcagc gtctttttta tcccaaattt gcaattccca aggataataa aagttacttg
    19741 aattcttaaa ataaatatga atacccacat attcatcttt atctctcaaa taccaatttt
    19801 ttagtccata tttttcttgc caataatcta atgactccat tacttgctga atcacttcac
    19861 tagaaacaat aatacgagct ccaaaaatat cgttgagaat gctatttact ggataacctt
    19921 cactacgctg tttaaaacgc tcgattttat ctaaaatcga ttctgaggtt tttactcggt
    19981 aaaaataaga aattccctct aaatctgcac gatataaata atcattaata gattcgtgta
    20041 aattaagacg ataagctaaa atatgtggcg taggcacttt ggcaaaagta tgcttaagat
    20101 tcactttttc aatcttacct gtttcaaagt agtctttcga aaaagctaag tgcaacttgt
    20161 tgatttcatc aatgagtttt tctattttct caagcatgct attcactctc taatttatta
    20221 tcttgcccca aatccagtta gcattgtttt caatgtttta aaaataatta aaatatcaag
    20281 ccataaagaa aaatgcttaa tatagtagaa gtcatgttct acttttactt ttgtatcatc
    20341 aacatctgcc gcataacctt ggacgacctg agcccaaccg gaaatacccg gtttaacgat
    20401 atggcgataa ttatagaatg gaatttcctc ttcaaattta tctacaaagg ctgtctgttc
    20461 tgggcgagga ccaattaagc tcatatcacc ttttaataca ttaaaaaact gaggtagctc
    20521 atcaatacga gttttacgga taaatttccc aatacgagtt accctcatat ccgcactcga
    20581 agcaaactgt gcatcatctt tttcggagtc tttacacata ctacgaaatt tataaatatt
    20641 aaactccttt ccaccttgcc caacgcgttt ttgaataaac attgctccac cttcactttc
    20701 taagcgaata gcaatagcgg tgattgtcat aataggtaaa gtaatgggaa acgtagcaac
    20761 aatcaatgca atatcaatga tacgcttcac taatgagtaa attggagaag gaagtaatga
    20821 acctaagtca ttttcataca tatggcgaat tttgacacgc ccggttaaag attcctcaag
    20881 ctggcgaacg ttatatacag gaataccatt caatgtacat ttcgctaaaa atttatgcca
    20941 ttctgcggac aaatcaggat agtgtaaatc taccgcaata gagttaattc tcttttcccc
    21001 caatattggt tcacttaatt gaatccactc tacattctga attcggttca aattgatagc
    21061 tcttccgaat ggaaccaaag cgattttagg gataatccaa cttcttgaaa gaaaataacc
    21121 aataaaacaa aatagaattg tgagtataaa gctcgtacct agcaatgtat tagaataatc
    21181 aaatctaaat attgttaaca cgattaataa aaccgcatac caagaaataa cggtggaaaa
    21241 aacaaaactt gctgacttat tacctggata tttcattaac actcttaagc tgatagctat
    21301 cattacaaag gcaagagaag tcgctataat cgtattagaa cgagtttcaa tagaattaaa
    21361 tttctctccc caaaaataga tagcgggtaa gacacttgag aacataacgc ccaaaatact
    21421 ttgaacagct gtactaaaaa agatacgttc ataccaacga gagtgtcttt tcttttgctg
    21481 aatgctcata atttatcctt ttcttaattt cttataaagt cttcctaaaa gagatctagg
    21541 caataaccgt ggtaaggctc taagtataaa aaacatgctt gatgagatta ttgattgtaa
    21601 tcccaactct tttttcaatt ttgctaattg gtactcactc ttaatgtaat tccacccttt
    21661 acgccgagcg tacattgcag aacctgaacg aacatttacc agtacatctg gtaaattata
    21721 cacctcatat ttctgtgtaa taactcgtaa ccataaattg taatcttcca tataaagatg
    21781 atgctgatag cctccaacat gctcgatcac agatttttta taggctactg ccatatggtt
    21841 aaaaggattt cttagtaatg ctgaaatacg aatttcatca tcattaatgg gcacttgttt
    21901 tatacccaga gaattagaca ttgtttcgtc aaattcttca acttgtcccc ccaataaaac
    21961 cacatcagga tttttactaa taaaatcaat ttgcttttca aatcgagatg gtaagcaaat
    22021 atcatcagta tccattctaa acacccactc attagagcag tgttttaagc cttcatttaa
    22081 ggccggccta aacccacatt ttgtggtaaa ctaacaacaa caagcggtaa tttttcagta
    22141 aaatgttgca caattcgctc taaatctcca ggaattatac catcaaaaac cacaacaatt
    22201 tcacttgctg gtaaagtttg ggatactaaa gaatctaaac acatctctaa ataagctggt
    22261 ttttctttat aatacaaaga
//
LOCUS       DANLZC010000024        22109 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.024, whole genome shotgun sequence.
ACCESSION   DANLZC010000024 DANLZC010000000
VERSION     DANLZC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22109
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.024"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(110..952)
                     /locus_tag="PXE44_000732"
     CDS             complement(110..952)
                     /locus_tag="PXE44_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDL5588268.1"
                     /translation="MKVGLVLEGGGLRGIFTAGVLDVFLDEHIHIDGAIGVSAGALFG
                     VNLPSKQKGRALRYNKKYAKDKRYMGLHSLLTTGNIVNRDFAYYELPSKLDPFDQQAF
                     EDSGMDFWVTVTNVETGEPEYIKIDNVFEQMEAFRATSSMPIVSKIVEIEGKKYLDGG
                     VSDSIPVQKFIEMGYDKIIVVLTRPLEYRKKPSSMWLFKRFYKKYPKLVQRWENRYAE
                     YNQAVEQIIQLNEKQQIFVIRPSRTVKISRLETDVNKIQAMYDLGVEDAKNALAGLRA
                     YLAK"
     gene            1012..1329
                     /locus_tag="PXE44_000733"
     CDS             1012..1329
                     /locus_tag="PXE44_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL5588269.1"
                     /translation="MADAEFMDFLLDQLSPISGLRSKKMFGEYCLFFDNKIVAIISKD
                     YRIFVKANPQTLPLFLAENAEQFHYFAKGKVQKMHYWSIPELAIEEADELVKWIRLGL
                     QAV"
     gene            1419..2132
                     /locus_tag="PXE44_000734"
     CDS             1419..2132
                     /locus_tag="PXE44_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HDL5588270.1"
                     /translation="MNQKKLVSLIVVIVLAIISQIFSFFDKKEPSKAQEKSTQSSQSH
                     QVSTSSLGNYDVQMKNDRLKQHIVDTDYYTLALSWSPAFCQKQKRNNHGKIPSHLEYQ
                     CGGSADFGWVIHGLWPQSGKARSVDEHPRYCQGDLPMVAEQTIKQYLPESPGATLLQG
                     EWEKHGACAFNQADDYFAKQKELFNQLKLPDTEMSKLALFNWMRANNPHLQNVYLGGS
                     KDELYICYDKAFQPMDCPR"
     gene            2132..2626
                     /locus_tag="PXE44_000735"
                     /pseudo
     CDS             2132..2626
                     /locus_tag="PXE44_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
     gene            2731..4011
                     /locus_tag="PXE44_000736"
     CDS             2731..4011
                     /locus_tag="PXE44_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL5588271.1"
                     /translation="METIVIVGGGAGGLELATYLGNKLGRKQKAKVVLIDRNATHLWK
                     PLLHEVATGTIDEGTDALSYRAHAKNHSFEFQQGTLVAVDREKKTVSLAPIYNDQNQL
                     LVKERHISYDKLVIAIGSKSNDFGTKGVAENCIFLDGSEQAKDFQKRMMELFLRFSHS
                     ENKDVKIAIVGGGATGIELSAELYHVVKNLNSYGFGKLNRASLKVTLIEAGPRLIPAL
                     PEKVSVSAFHELKKAGVSVRLGTMITEARPDGLMTRLGEKIEADIMVWAAGVKAPEIT
                     REFGFETNRLNQIEIKDTLQTTVDESVFVIGDCASLIQDGKPIPPRAQAAHQMATRCG
                     KNIVALIEGKEMKPFRFNDKGSLLSFSKFGTVGNLMGNLVSGDMFIEGKIARLAYLSL
                     YRMHQVALHGCVKTGLIVLVGQINRFLRPTMKLH"
     gene            complement(4098..5135)
                     /locus_tag="PXE44_000737"
     CDS             complement(4098..5135)
                     /locus_tag="PXE44_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5588272.1"
                     /translation="MNVLKLISLKSILIASGIFLSAQAAQAEGRLTVYCSVQNNVCEE
                     MTKQFSHKYNVETKFIHGGTGTIFGKVKAEASNPQADIWYGGTIEPHFQAGELGLLEA
                     YRSPKQAEILPQFKSLVESEQGKFTSIVYMLVLGLGVNTEKLAKLGIEAPKKWEDLSD
                     PRLKGEVQLPDPRSSGTTYTFMTTLSTLWGEEKTFDYLKKLNNNVSQYVKSTLVTSNL
                     ARGESAVTVGFVHSYATEKEKGAPIEAIIPEGKVGYALGGVSIIKNARNLDNAKLFMD
                     WVLSKEAQEIPWKKHGVYQTPTNINAEVAPQSTKAEALDFVEIDYQKAGSSEEGKRLI
                     DKWLAEIKLAN"
     gene            5357..6055
                     /locus_tag="PXE44_000738"
     CDS             5357..6055
                     /locus_tag="PXE44_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL5588273.1"
                     /translation="MENSWKYAFKLITPIFMGYFAAGVAYGMLATNAGMPAWFTIVMS
                     FTVVSGTAQYAAIPFFVSGVGAVSVFLSTLLMSLRFSFYTLNMMEHKPISKAKSLVAI
                     AGLTDEGFAVLSSLPKEQKQALFFKIAVLCVAYWTFSTIVGVLLGDSVANYIPHLDFA
                     LPCLFAILAYEQYKNQKQWKPIVIALIGFLLARQITETSVLLVAILVAIVIVALLPQS
                     SSIKLEKGVENEHK"
     gene            6066..6368
                     /locus_tag="PXE44_000739"
     CDS             6066..6368
                     /locus_tag="PXE44_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL5588274.1"
                     /translation="MFAALVLGSQICRFLPEFLPKKVLSSPILQKLNKMLPLVIMLLL
                     LLTSLNFPKNGEGFNLILAQILALIGVLGSYVWLKNTFLSVALGIFGVNVFLSLLG"
     gene            complement(6489..7790)
                     /locus_tag="PXE44_000740"
     CDS             complement(6489..7790)
                     /locus_tag="PXE44_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL5588275.1"
                     /translation="MSLFKFEERGSNARQEIIAGLTTFLAMVYSVIVVPGMLSQAGFP
                     AESVFIATCLVSGLGSILIGLWANAPMAIGCAISLTAFTAFSLVLGQQVSIPVALGAI
                     FLMGVVFTLISATGIRTWILRNLPSSIAHGAGIGIGLFLLIIAATNVKLVVNSGMDIP
                     VKLGDFTSFPVLVSLIGLAAIIGLEKLKVKGSILWVIIAITIVGLIFDPNVKYTGFFK
                     MPSFGENSQFLNLDIMGALNTAILPVVFALVMTAIFDATGTIRAVAGQAGLLDKDGQI
                     INGDKALTSDSFGSILSGLFGTAPAAVYIESAAGTAVGGKTGLTAVVVGIGFLFMLFF
                     QPLAFLVPSYATAPALMYVGLLMLSNVSKLDFSDFVGAMSGLVCAVFIVLTANIVTGI
                     MLGFATLVIGRLISGEANKLNLGTVIIAIILVAFYAFDLAI"
     gene            complement(7923..8897)
                     /gene="secF"
                     /locus_tag="PXE44_000741"
     CDS             complement(7923..8897)
                     /gene="secF"
                     /locus_tag="PXE44_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDL5588276.1"
                     /translation="MAIENVQQDVAEIKLPYKLIPFMKYRYVGFIFSLIVTALCIFTI
                     ATKGFNWGLDFTGGTVIETQFSQPADLAKLRTELENSGYGNALVQTTGSQKDLMIRLA
                     ASAGDMSVGNKVMEMIHQKLDPNATIKSIEFVGPNVGEELTQGAIYATLATLAMLLIY
                     VGTRFEWRLAVGGIVALFHDVLVTIGIFSFLQIEIDLTFVAAILSVVGYSLNDSIVVF
                     DRVRENFSKVRRSSSIEIIDISLSQTLSRTLMTSVTTLFVVLALFYLGGPTLHSFSLA
                     LLIGIGFGTYSSIYIAIGVALQLGLKREHMVQPKVEKEGADQDAFIDY"
     gene            complement(8907..10748)
                     /gene="secD"
                     /locus_tag="PXE44_000742"
     CDS             complement(8907..10748)
                     /gene="secD"
                     /locus_tag="PXE44_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDL5588277.1"
                     /translation="MLNRFPLWKNLMVIFVIAIGALYALPNLYGDDPSVQISGTRGQQ
                     ATPETLSQVQSTLSAMNIQPKSAVLENGSILVRLESSEQQLPAKEKISEALGNNYSVA
                     LNLAPATPQWLSDIGGSPMKRGLDLLGGVRFLMEVDMNTALAKQQDTLQDSLRSDFRK
                     EKLQYQAVKKGENFATEVEFADNDTAEKAVRLVRLSHPTLDILVNDNKVTLTPSAQAL
                     SESRTMAIEQNLSILRKRVEELGVSEPTIQRQGADRIVVELPGVQDTARAKELLGATA
                     TLEFRLVNTEANVASAAKGIVPADSGLQNTRDGEPVVLRRKPSLGGEHIIDATSGTDE
                     RGLPQVSIRLDTEGGEMMGEITKMAIQKPMATLYTEFKDSGRKDANGKVILEKHQEVI
                     NVATIQTRLGNQFQITGINSPAEAQNLAVLLRSGALIAPIVIVEERTIGASLGADNVK
                     QGMEASILGLGLTILFCLAFYKVFGIFASLALIVNMILTIGLMSLIGATLTMPGIAGI
                     VLAVGMSVDANVLIYERIKEEIRNGRPIQQAIHEGYNGAFSSIFDSNLTTILTSLVLY
                     GVGTGPVKGFAVTLALGVIISMFTAITGTRMLVNWVYGGKRVKKLWI"
     gene            complement(10788..11081)
                     /gene="yajC"
                     /locus_tag="PXE44_000743"
     CDS             complement(10788..11081)
                     /gene="yajC"
                     /locus_tag="PXE44_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL5588278.1"
                     /translation="MQQGGGMEMIFILIIFGAIFYFMIYRPQAKRQKQQRDLLASLTK
                     GDEVLTSGGLIGKITKVTADNDNIVIALNDTTEVTIKRDFVVAVLPKGSLKSL"
     gene            11282..11839
                     /locus_tag="PXE44_000744"
     CDS             11282..11839
                     /locus_tag="PXE44_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDL5588279.1"
                     /translation="MKKLFAQAVIFGAFFASSAFAHNVQLNQALPAVSVAKDGELAVA
                     GGKVSYKNWHSSSLAGKVRVIHHFAGRSSVKEKNEDLMTAIKNAGFDRSKYQTVTIVN
                     ADDAIVGTGVFVKNSVENGKLDNPHSQVVLDQQGSVKKAWGLKEKESFIAVLDKSGKV
                     QFTSEGKLSAKQIQEVIGLVKTLTQ"
     gene            complement(11880..12848)
                     /locus_tag="PXE44_000745"
     CDS             complement(11880..12848)
                     /locus_tag="PXE44_000745"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDL5588280.1"
                     /translation="MKLFTLENAFLRITLSDVGASWLSCIVKMPNEEREVLVTTSAKN
                     WQKQTAFFGATIGRYANRIANAEYRLNGKTYQLAKNNGEHNLHGGELGADKIVWQLES
                     QSEQAVKFSYFFADGEEGFGGEVKAFVEYRLTENELQISFDAVANQDTPLCLTNHAYF
                     NLQGSETILDHELIINADGYLPVDASGIPNAPLKKVESTSFDFRNAKKIGQDLLADDD
                     QKAVKGYDHSFHLAKNSENPTACLAVEDLKMELRTSMPALQLYTGNWLKGQPNLTKGE
                     YEDYAGVALEPEFFPNSLNQPELLQFGGITKAGEEYHHTISYRFII"
     gene            complement(12974..14137)
                     /gene="galK"
                     /locus_tag="PXE44_000746"
     CDS             complement(12974..14137)
                     /gene="galK"
                     /locus_tag="PXE44_000746"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL5588281.1"
                     /translation="MKPQEIALQQFEQHFGYKAERTVFAPGRVNIIGEHTDYNDGFVM
                     PCAINYGTAVSFAKRSDNKWRVFAIDLNQQDEFDLSQAIEPSEQKWANYVRGVVKFIQ
                     AKHPEFKQGADLAITSDVPMSSGLSSSAALEVSIGKTAQLLGDLPLSLPQIALIGQEA
                     ENKFVGCNCGNMDQLTSALGQQDHLVMIDCRSLEITPTPVPQGYSIAIINSNVKHDLV
                     TGEYNSRREQCEFAAHFFGKAKLRDVTAEQFIERASELSEENELAYKRAKHIISENQR
                     VLEAVEALKANDMVKLGELMAQSHDSMRDDFEITCPEVDCLAEIAQQAIGKNGGARMT
                     GGGFGGCVVCLVPNDKVEDLRQAVANNYEKQTGIKETFHVCTASDGVRECPAR"
     gene            complement(14147..15193)
                     /gene="galT"
                     /locus_tag="PXE44_000747"
     CDS             complement(14147..15193)
                     /gene="galT"
                     /locus_tag="PXE44_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDL5588282.1"
                     /translation="MSEFVLNDHPHRRFNPLKNQWILVSPHRAKRPWQGQQEEIVQDE
                     KPSYDPSCYLCPGNKRITGEQNPNYTKPFVFKNDFSALLPDTPAPIPNDDPLFQISHT
                     QGESRVICFSPDHSKTLPQLSIEEITDVVTVWQEQANELKEHYQWVQIFENKGSMMGC
                     SNPHPHGQIWASNFLPNEIAIEDECQAKYFAQYGSPLLLDYAQRELDKQERIVAETED
                     WIAVVPYWAAWPFETLLLPKAKQFKSITDLNEAEKADLALALKKLTTRYDNLFNISFP
                     YSMGFHFAPFNGKENAHWQLHAHFYPPLLRSATVRKFMVGYEMMAESQRDLTPEQAAE
                     RLNAVSDTKHYKDK"
     gene            15406..16257
                     /locus_tag="PXE44_000748"
     CDS             15406..16257
                     /locus_tag="PXE44_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HDL5588283.1"
                     /translation="MERLNKFDAWLMNHKPNARLSGASRLLTEFWFFGLKEARACLFA
                     GLFFIAMFSVPKTGLWGLPRYDILLIFAIVVQIAMVKLGLETWDELKAISLFHLVGFV
                     LEVFKTSSNIQSWAYPDFAYTKLFNVPLFTGFMYAAVGSYIIQAWRLFDLKIKSHLPY
                     WLATLTALMIYLNFFTHHYIGDYRWYIAAFALGLYARTFVFFTPYDITRKMPLLLSFF
                     LIGFFIWLAENIGTFAGIWRYPNQFGAWSMVHMSKWSAWALLVMMTFTIIVHLKHIKQ
                     TVRVSSD"
     gene            complement(16343..17428)
                     /locus_tag="PXE44_000749"
     CDS             complement(16343..17428)
                     /locus_tag="PXE44_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5588284.1"
                     /translation="MEYRYYITTALVIAVLQLFLFNFAKTVSWLFNLSSKNRKTVGIL
                     TFLVANGLVILHLTRTFALFRIMAFILVLLLFAFFISFFTACIYKIFNGNTTVNRWLK
                     IAYPFAFFGLLGLGYYNAYTPTVVHYSVKLDKPLEKPLRIGMASDLHLGKLFGTKQLD
                     QLAEIFNQQKVDLILLPGDIMDDNTEVYVADKMQPHLAKLKAPLGVYATLGNHDFFGA
                     QETIAKEITDAGITVLWDQTAEINGKFTLIGRNDDLVKNRPSAEQLLQNVNTKLPVIL
                     LDHRPTEIEQHAKLPIDIQVSGHAHKGQVFPASIITKLIYRLHYGYEKIGFGHYFVTS
                     GYGFWGVPMRLGSQSEVMIIDVEGENP"
     gene            complement(17450..18430)
                     /locus_tag="PXE44_000750"
     CDS             complement(17450..18430)
                     /locus_tag="PXE44_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL5588285.1"
                     /translation="MLFDSIQFSIGVTLPTILLMLFGIFPRRRKFIDDTFCNMASKVV
                     FNFTLPPMIFLNMLKSPLDFSSQINLISTGVGGSILIYLWAEWWAARHIENRRYRCIF
                     TQGTFRTNAAILGIALILNAYGDKALAAASVYISCLVITFNVLAVITLTSSLSEKKVN
                     FGRLILSILRNPLILAVIAGLTFNYFELSHQIPTPLESAGKLMASLTLPLALICTGAS
                     INFKQLKQFNQGVESTINKIVLFSASIRLIFAPIFLLLLGKFVFQLPPMELGIVFVAA
                     SAPVASATYAMTRNYGGDGEAAANLIGITTLGSMFSASIGLFLLRQIGWV"
     gene            complement(18533..19631)
                     /gene="prfB"
                     /locus_tag="PXE44_000751"
     CDS             complement(join(18533..19555,19557..19631))
                     /gene="prfB"
                     /locus_tag="PXE44_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550889.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL5588286.1"
                     /translation="MFELNPIKNQLTDLSERTAVIRGYLDFEHKVERLEEVNAELEQP
                     DVWNKPEKAQALGKERAMLEQIVNTIKALEQGIEDVEGLIELAVEAEDQDTFNEAQVE
                     ADELEKKLADLEFKRMFSGPHDSADCYLDLQAGSGGTEAQDWTEMLLRMYLRWAESKG
                     FKAELMEASDGDVAGIKSATVKISGDYAYGWFRTETGIHRLVRKSPFDANNGRHTSFS
                     AAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQN
                     GRSQHQNKDQAMKQLKAKLYELEMSKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DHRTGVEHRTTQQVLDGDLDKFVEASLKAGL"
     gene            19887..20795
                     /locus_tag="PXE44_000752"
     CDS             19887..20795
                     /locus_tag="PXE44_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDL5588287.1"
                     /translation="MAKRPSDIDATLLQLEILRRIPKLPAKIDAKSLHQQLLDAGFER
                     DIRTVQRSLKMLCEHFDIECDERSKPFGYSWKIRSEGLALPILNEQQSLLLKLAEQQL
                     KYLLPANLMASMKPFFEQADRMVSGAKDKPEYQWLNKVCSTPTSLPLIPAKIKEEIFN
                     SVSQALFQNKLLKIEYQNQHGKKHQATIMPLAISQQGASIYLVCRYEGFDDNRLLALH
                     RIRKAEISTFSFERPKDFNLQAYQDAGHLGFGNGEKASLTFSISRDAGFHLTETPLAK
                     DQKILEESETHYRIQAIVADNDMLTW"
     gene            21096..21737
                     /locus_tag="PXE44_000753"
     CDS             21096..21737
                     /locus_tag="PXE44_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDL5588288.1"
                     /translation="MPFQLTFCQQAGNQHQKNQDALFNGKAVYQWQLKNAESYLLDND
                     KVIFGVADGVSSHPRNHLASHYLMALLSQCQSLDSQWLRQAHGDFCEKLAKDYFGASC
                     TFVACELHHNGRGRMVNVGDSRAYKITANGEWQQLSFDHTILAEMKQKGLVKEGSNYA
                     SMYNGLTDCIVADFDADDFRIHIAEFQLEKGESILLCSDGLHEQLAPEAIQAI"
     gene            21904..>22109
                     /locus_tag="PXE44_000754"
     CDS             21904..>22109
                     /locus_tag="PXE44_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDL5588289.1"
                     /translation="MKNDFHYAAELIRNADGILITAGAGMSVDSGLPDFRSVGGLWNS
                     YPPLKKFNLQFMQIATPLAYQERP"
BASE COUNT         6342 a         4553 c         4694 g         6520 t
ORIGIN      
        1 aaaatgtttg gacaggcaca ggaccggtcc ctataaaatt tgtttggaag tgtttccgtc
       61 tagacgtcta taatatattt tttgaacgtt ttaggcaaat agaatttgtt tatttggcta
      121 aatatgccct taaaccagcc aacgcatttt tggcatcttc tacccctaaa tcgtacattg
      181 cttgtatttt atttacatcc gtttcaaggc gactgatttt gactgttctg cttggacgaa
      241 tcacaaaaat ctgttgtttt tcattgagtt gaataatctg ctcaacggct tgattatatt
      301 ccgcatagcg gttttcccag cgttgcacta atttcggata ttttttataa aaacgtttaa
      361 acagccacat cgagcttggt ttcttacggt attcaagtgg gcgagtaagc accacgataa
      421 ttttgtcata tcccatttca ataaatttct gcaccggaat actgtctgac acaccaccgt
      481 ctaaatattt tttaccctca atttcgacta ttttggatac aatcggcatt gaggaagtag
      541 ctcgaaaggc ttccatttgc tcgaacacat tgtcaatttt gatgtattcg ggctcaccgg
      601 tttcgacatt ggttacagta acccaaaagt ccataccgga atcctcaaag gcttgttggt
      661 caaacggatc cagtttggaa gggagttcat agtaagcaaa atcccgatta acgatattgc
      721 cggtggtaag caagctgtgt aaccccatat accgcttatc tttagcgtat tttttgttgt
      781 atcgcaacgc cctacctttt tgttttgaag gcaaattcac gccaaataac gcaccggctg
      841 aaacaccgat tgctccatct atatgaatat gttcatctaa aaatacatct aacacgccgg
      901 cagtaaaaat acctcgcaaa ccaccgcctt ccagcactaa tccaactttc atatcattct
      961 ccctgtaatt tgtgtattat aacccgaatt atcttgctag gagaaatcac aatggctgat
     1021 gcagaattta tggatttcct gttagatcaa ctaagcccga tttccggttt gcgttcgaaa
     1081 aaaatgtttg gtgaatattg cttgtttttt gataataaaa ttgtcgctat tatcagcaaa
     1141 gattatcgca tttttgtcaa agctaatccg caaaccttgc cgttattctt agctgaaaat
     1201 gccgaacaat ttcactattt tgctaaagga aaagtgcaaa aaatgcacta ctggagcatt
     1261 ccggaattag cgattgaaga agccgatgaa ctggtcaaat ggattcggct aggcttacaa
     1321 gcggtgtaat ttagcgaaaa ttttgtaaaa aaacgctaaa atatgcccgc ttgttagggg
     1381 ggggacctaa tatttctata ctttaagatt ttatatttat gaaccaaaaa aaattagttt
     1441 ctttgattgt cgttattgtg ttggcaatca tttctcaaat ttttagcttt tttgataaaa
     1501 aagagccttc aaaagcacaa gaaaaatcga cccagagtag tcagtctcat caagtttcaa
     1561 cttcatcact agggaactat gatgtgcaga tgaaaaacga ccgtttgaaa cagcatattg
     1621 tagataccga ttattacacc ttagcgttgt cgtggtcgcc ggcgttttgc caaaaacaaa
     1681 agcgaaataa tcacggtaaa attccgtctc atttggaata tcaatgtggt ggctcagcgg
     1741 attttggttg ggttattcac ggcttatggc cgcaaagtgg caaggcaaga agtgttgatg
     1801 aacaccctcg ttattgccaa ggcgatttgc cgatggtggc agagcagacg attaagcaat
     1861 atttacctga atcgccgggg gcaactttgt tgcaaggcga atgggaaaaa cacggtgctt
     1921 gtgcctttaa ccaagcggat gattacttcg ccaaacaaaa agagctattt aatcagctca
     1981 aattaccgga taccgaaatg tctaaattag cactgtttaa ctggatgcga gccaataacc
     2041 ctcatttgca aaatgtctat ttagggggta gcaaagatga gctgtatatt tgctatgata
     2101 aagcatttca accaatggat tgcccacgct aatggcagat cttcgccaac tgaaaatgca
     2161 ggttcagcgc caattaaagc gtgatcttga acgtgcaaat cactatttta ataccacttt
     2221 tacgccacca agtattagct atgcggtgcg tggcgtaaaa gccggtgtgg cttatcttga
     2281 acgcaacgaa gtacggttta atcctgtgtt attgtaagaa aatgaacagg catttatccg
     2341 ccaagttgtg ccgcacgaac tggctcatat cttggtttat caacattttg ggcgggtaca
     2401 atctcatggt aaagagtgga aaatgatgat ggaaaccgta ctgggtgtgc cggcggaaat
     2461 ttaccattgt tttgataccc aaagtgtgca acagcaattt agttaccaat gtgcctgcca
     2521 gactcaccag ctttcgattc gccgacacaa tgccgtgatg cgagataaaa aaagctatat
     2581 ttgccgttta tgtaaaactc aactccattt tgtagaaaaa agttaatgtg gagaaaagtt
     2641 aagagaatgt tacgatttag ctagacattc ctgtttaaac gcctacaatt tgccgtagtt
     2701 tttattttta attttatttc cggagtattt atggaaacaa ttgtaattgt aggtggcggt
     2761 gcaggcggtt tggagcttgc aacctatcta ggcaacaaat tagggcgtaa acaaaaggca
     2821 aaagtggtgc tgattgaccg caatgcaacg catttatgga aaccactgtt gcacgaggtg
     2881 gcaaccggta ctattgatga gggaactgat gcgttaagct atcgggctca cgctaaaaat
     2941 catagttttg aatttcagca aggtacgctg gtggcggtag atagagagaa gaaaacggtt
     3001 tcactcgccc cgatttataa tgatcaaaac caacttctcg ttaaagaacg ccatatcagt
     3061 tatgacaaat tagtgatcgc gatcggcagt aaatccaatg attttggcac caaaggggtt
     3121 gcagaaaatt gcattttctt agatggctca gagcaagcaa aagatttcca aaaacgaatg
     3181 atggagctgt ttttacgttt ttcacatagt gaaaataaag atgtaaaaat tgcgattgtg
     3241 ggggggggag caacaggtat tgagctttca gcggagcttt accacgtggt gaaaaattta
     3301 aattcttacg gctttggtaa attaaaccgt gccagcttga aagtcacttt aattgaggca
     3361 ggccctcgtt tgattccggc attaccggag aaagtgtcgg tttctgcttt ccacgaattg
     3421 aaaaaagcgg gtgtttcggt gcgtttaggt acgatgatta ccgaagctcg tccagatggg
     3481 ctaatgaccc gtttaggtga gaaaattgag gcggatatta tggtttgggc ggctggcgtg
     3541 aaagcccctg aaattacccg tgaatttggt tttgaaacta accgcttgaa ccaaattgag
     3601 attaaagaca cgctacaaac caccgttgat gagtcagtgt ttgtcattgg cgattgtgca
     3661 tcattaattc aagatggcaa gccaattcca cccagagcac aagcagccca ccaaatggca
     3721 acccgatgtg gtaaaaatat tgtggcattg atagaaggaa aagagatgaa gccgtttcga
     3781 tttaacgata aaggctcact gctttccttc tcaaaattcg gcacggtggg taatttaatg
     3841 gggaatttag tgagcggtga tatgtttatt gaaggtaaaa ttgcccgctt ggcttacctc
     3901 tcgctttatc gaatgcacca agtggcatta cacggctgtg tcaaaaccgg cttgattgtg
     3961 ttggtagggc aaattaaccg tttcttaaga ccaacaatga aactacatta aacaaaaatc
     4021 cgcatcaaat gatgcggatt ttttatggca aaataacaag cggttatttt cttgcctatt
     4081 tttgcaaata ctcacgccta gtttgctaat ttaatttccg ccaaccattt atcaattaag
     4141 cgtttgccct cttcgctgga acctgctttt tggtaatcaa tttccacaaa gtctaacgct
     4201 tcggctttgg ttgattgtgg agcaacttcg gcgttgatgt ttgttggggt ttggtaaaca
     4261 ccgtgttttt tccaaggaat ttcctgtgct tcttttgaaa gcacccaatc cataaagagt
     4321 ttggcgttat ctaaattacg ggcatttttg ataatgctga caccgcctaa cgcatagccc
     4381 actttacctt ccggaataat ggcttcaata ggcgcacctt tttctttttc agtggcataa
     4441 ctatgtacaa agcctaccgt aaccgcactt tcaccacgag caagattgct ggtgactagg
     4501 gtgcttttca catattgcga aacgttattg ttgagttttt tcagataatc aaaggttttc
     4561 tcttcgcccc ataaggttga aagagtggtc ataaacgtat aagtcgtgcc ggagctacga
     4621 ggatcaggca gttgtacttc accttttaag cgaggatctg ataaatcttc ccattttttc
     4681 ggtgcctcaa tgcctagttt ggcgagtttt tcggtattca cgcctaaccc taatacaagc
     4741 atataaacga tagaggtaaa tttaccttgt tcactttcaa ctaaggattt aaattgtggc
     4801 agaatttcag cttgttttgg tgaacggtag gcttccagta agcctaattc gcctgcttga
     4861 aagtgaggtt caatcgtgcc gccataccaa atatctgcct gagggttcga tgcttccgct
     4921 tttacttttc cgaaaattgt accggttccg ccgtgaatga atttagtttc gacattatat
     4981 ttgtgtgaaa attgcttggt catttcttca caaacattat tttgtaccga gcaataaaca
     5041 gttaaacgac cttctgcttg tgctgcttga gctgataaga aaatgccaga ggcgattaag
     5101 atcgatttta atgagattaa tttgagtacg ttcatcactt tcctgaaaac ataaaataaa
     5161 aggtagtgaa gtctagcatt tcaaagcgct ggttgaaaat agttaattgc aatgaagtag
     5221 ggcaattgtg cctattactt taatatttac caactatttg caagttttag caaaaaataa
     5281 ccgcttgtag tagctcttgc tattttgtca aaattcagaa taataaatgc ctttagtcta
     5341 atgttaaatt ttagttatgg aaaatagttg gaaatatgct tttaaattaa tcacgccgat
     5401 ttttatgggc tattttgcgg caggggttgc ttacggaatg cttgccacca atgccggaat
     5461 gcctgcgtgg tttacgattg taatgagttt taccgtggta tcggggacgg cacaatatgc
     5521 agcgattcca ttttttgtct ccggtgttgg ggcggtgtcg gtctttctca gtacgctttt
     5581 aatgagtcta cgttttagtt tttacacctt aaatatgatg gagcataagc cgatatccaa
     5641 agcgaaaagt ctggtcgcta ttgcaggttt aactgatgaa ggctttgcgg tgttatcttc
     5701 attaccgaaa gagcaaaagc aagcattatt ctttaaaatc gctgtgctat gtgtggctta
     5761 ttggacattt tccacgattg tcggtgtgtt gttaggcgat tcagtggcga actatattcc
     5821 gcatttggat tttgctctgc cttgtttgtt tgcaatttta gcctatgagc aatacaaaaa
     5881 ccaaaaacaa tggaaaccga ttgtgattgc gttaatcgga tttttacttg ctcgtcagat
     5941 taccgaaacc agcgtgttat tggtggcaat tttagtagcg attgtcattg tggctttatt
     6001 acctcaatct tcttcaatca aactagagaa aggagtagaa aatgagcaca aatgatactt
     6061 tgataatgtt tgcagcatta gtgctcggct cgcaaatttg tcgttttttg ccggaatttt
     6121 taccgaaaaa ggtgttatct tcgcccattt tgcaaaaatt aaacaaaatg ttaccgcttg
     6181 tgattatgct tttattgctt ttaacgagcc taaactttcc taaaaacggg gaaggcttta
     6241 acttaatatt ggcacaaatt ctggctttaa taggcgtatt aggtagctat gtttggctta
     6301 aaaatacctt tttaagcgta gcgttgggga ttttcggtgt gaatgtattt ttatccctgt
     6361 taggataaat accaaaggtc attttatagg agcgaaacac cttcgctcaa tcattaacag
     6421 atgtggggcg aaaaattttt cgccccttat ttatcacaag cggtcttttt ctttcaattt
     6481 tttgcaaatt aaatcgctaa atcaaacgca tagaaggcaa ctaaaataat cgcaataatt
     6541 actgtaccta ggtttaattt attggcttcg cctgaaatta agcgaccaat cacaagggtg
     6601 gcgaaaccta gcataatgcc tgtcacaatg ttggcggtta atacgatgaa caccgcacag
     6661 actaaacctg acattgcacc tacaaaatcg ctgaaatcta atttagagac attacttagc
     6721 attaataagc ccacatacat taatgccggt gcggtggcat agcttggtac taagaatgca
     6781 agaggttgga agaacagcat aaataagaaa ccgataccaa ccactacagc ggttaaacct
     6841 gttttaccgc caacggctgt acctgctgcc gattcaatat acactgccgc tggtgctgta
     6901 ccaaataagc ccgataaaat tgatccgaat gagtcagaag ttagggcttt atcgccattg
     6961 ataatttgtc cgtctttatc taataaaccg gcttgccctg caaccgcacg gatagtacct
     7021 gttgcatcaa aaatagcggt cattactaac gcaaacacta ccggtaaaat ggcggtattt
     7081 aatgctccca taatatcaag atttaggaat tgcgaatttt caccaaaact cggcattttg
     7141 aaaaagcctg tgtattttac gttagggtca aaaattaaac ctacgatggt aatagcaata
     7201 atgacccata aaatactgcc ttttactttc agcttttcta acccgataat ggcggctaga
     7261 ccgattaatg acactaaaac cggaaatgaa gtgaagtccc ctaatttaac cggaatatcc
     7321 ataccgctat tgaccactaa tttaacgtta gtggcggcaa taatcagtaa gaataaacca
     7381 ataccaattc cagcaccgtg ggcaatgctg cttggcaaat tacgtaaaat ccaagtacga
     7441 ataccggtag cagaaatcaa ggtaaatact acgcccatta agaaaatcgc cccgagtgca
     7501 acaggaatag aaacttgttg ccctaatact aagctaaagg cagtaaaagc agtaagtgaa
     7561 atcgcacaac cgattgccat tggggcattt gcccataaac cgattaagat agagccgaga
     7621 cctgaaacta aacaggtggc aataaatacc gattcggcag gaaagcctgc ttggcttagc
     7681 attcccggca caacgatgac ggaataaacc atggctaaaa aggtagttaa accggcgatg
     7741 atttcttgac gagcgtttga accacgctcc tcaaatttaa ataacgacat aagaacctca
     7801 aaagaagaaa atgaaagggg aaattttagg atgcggaatt ttacagaaat ttaaaataat
     7861 tagcaaacgt ttgcgtaaaa agtgaaagaa aagcaggaat aaatcctgct tttgtttgat
     7921 ggttagtaat cgatgaaagc gtcttgatct gccccttcct tttcaacttt aggctgaacc
     7981 atatgttcac gttttagccc aagttgtagt gccacaccaa ttgcaatgta gatggatgaa
     8041 taagtaccga aaccgatacc gatcaataat gcaagtgaga aactatgaag cgttggacca
     8101 cctagataga ataaggctag taccacaaag agggtggtta ctgatgtcat taaagtacgc
     8161 gaaagtgttt ggctcaagga aatatcgata atttcaattg agctggagcg acgaacttta
     8221 gaaaagtttt cacgtacacg gtcaaacacc acaattgagt catttaacga gtaaccgaca
     8281 acagataaaa tcgctgctac aaaggtcaag tcaatttcaa tctgtaagaa agagaagatt
     8341 ccgatcgtca ctaatacatc gtgaaataaa gccacaatac cgcctaccgc taaacgccac
     8401 tcaaaacgtg ttcccacgta aataagtagc atagcaagtg ttgccaaagt ggcataaata
     8461 gccccttggg ttagctcttc ccccacgttt gggccaacga attcaatgct cttgatagtc
     8521 gcattcggat ctaatttttg atggatcatc tccatcactt tattcccaac agacatatca
     8581 ccggcactgg cggcaagtcg gatcattaaa tctttttgac tgcctgtggt ttgcactaag
     8641 gcattgccgt agccgctatt ttcaagctcc gtacgcagtt ttgctaaatc agcaggttgt
     8701 gaaaattgag tttcgattac tgtcccgccg gtaaaatcta acccccagtt aaagcctttt
     8761 gtcgcaatag taaaaatgca taaagcagtt acaatcagcg aaaaaatgaa tccgacatag
     8821 cggtatttca taaaggggat taatttataa ggcagtttaa tttctgctac atcttgttgt
     8881 acattttcaa tagccatttt tccaccttaa atccaaagtt tcttcacgcg tttgccaccg
     8941 taaacccagt ttacaagcat tcgagtacct gtaattgcgg taaacataga aataattacc
     9001 cctaatgcaa gcgttaccgc aaagcctttt accggacctg tacctacacc ataaagcaca
     9061 agagaagtca aaatagtggt gaggttggag tcaaaaatac ttgagaatgc gccattataa
     9121 ccttcgtgga ttgcttgttg aattgggcga ccgttacgaa tctcttcttt gatccgctcg
     9181 tagattagta cgttggcatc aactgacata cccacagcaa gtacgatacc ggcaattccc
     9241 ggcatagtaa gggttgcacc gattaatgac attaaaccaa tcgttaagat catattcaca
     9301 atcaaggcaa gagaggcaaa tataccaaat actttataaa aggctagaca gaacaggatc
     9361 gttaagccta agcctaaaat actagcttcc ataccttgtt taacattatc tgccccaaga
     9421 gaagcaccga tagtacgctc ttccacaatc acgattggag caatcaacgc acctgaacgt
     9481 aataataccg caaggttttg agcttccgct ggtgaattaa tccctgtaat ctggaattga
     9541 ttacctaaac gggtttgaat ggttgcaacg ttaattacct cttggtgttt ttctaaaatg
     9601 actttaccgt tagcatcttt acgacctgaa tctttaaatt cggtataaag tgttgccatc
     9661 ggtttttgga ttgccatttt agtaatttca cccatcatct cgccgccttc ggtatcgagt
     9721 cggatactca cttgcggcaa gccacgctca tcagtgcctg aggttgcatc aataatatgt
     9781 tcacccccta acgaaggttt acggcgtaat acaactggct cgccatcacg ggtgttttgc
     9841 agtcctgaat cagccggcac aatgcctttc gccgcagagg ccacattggc ttcggtattg
     9901 actaaccgga actcaagggt tgcagttgcc cctaaaagct ctttggcacg agcggtatct
     9961 tgcacacccg gtaattcaac tacgatacga tctgcacctt gacgctgaat ggtcggctca
    10021 gaaacgccca attcctcaac acgtttacgc aaaatagaaa ggttttgttc gattgccatc
    10081 gtgcgagatt cagaaagagc ttgtgctgat ggcgttaagg tgactttatt atcatttacc
    10141 aaaatatcaa gcgttgggtg agagagacgc actaaacgca ctgctttttc ggcggtatcg
    10201 ttatcggcaa attcaacctc agttgcaaaa ttttcgcctt ttttgaccgc ttgatattgc
    10261 aatttttctt tacggaaatc actacgcagg ctgtcttgca aagtgtcttg ttgttttgca
    10321 agcgccgtgt tcatatccac ttccattaag aaacgaacac cgccaagcaa gtccaagcct
    10381 cgcttcatcg ggctaccgcc aatgtcagac aaccactgtg gcgttgctgg tgcaaggttt
    10441 aaggcaacag agtagttatt ccccaatgct tctgagattt tttctttggc aggcaattgc
    10501 tgttcgctgc tttcgagacg aaccaaaatc gagccatttt ctaacacggc agatttcggc
    10561 tgaatattca ttgctgaaag tgtggattgc acttgagaca gtgtctcagg tgtggcttgt
    10621 tgcccacgag taccggaaat ttgtaccgaa ggatcatcgc catataaatt tggaagggca
    10681 tataaagcac caatggcgat cacgaagatc accattaaat tcttccatag agggaaacgg
    10741 tttaacacaa taactccccg aatgttaaag ttggataaga gggttgatta aagtgatttt
    10801 aatgaacctt ttggtaacac cgcaacaacg aaatcacgtt tgatggtcac ttcagttgtg
    10861 tcatttaatg caatcacgat gttatcgtta tcagcggtca ctttggtaat tttaccgatt
    10921 aaaccaccgc tggttaatac ttcatcgcct tttgtcaagc ttgccagtaa atcacgttgt
    10981 tgtttttggc gttttgcctg aggacgataa atcataaaat agaaaattgc accgaaaata
    11041 atcaggataa agatcatttc cattccgcca ccttgttgca taaaaactcc ttaaatgttt
    11101 gagtggtata aaaataagtg ttataaaaaa cgcctgttaa aataccataa attcaatgaa
    11161 ttaacgagta gaaaaattca tcgaatgctt ttcttttaaa caaaaatttg agtagaatca
    11221 gcacttttag tttaggatta ggttcttggc gtgttcttgc gctgacacaa taggagaaaa
    11281 aatgaaaaaa ttattcgctc aagcggttat ttttggggct ttttttgcaa gttccgcttt
    11341 tgctcacaat gtgcaattaa atcaagcatt acctgctgtt tcggttgcaa aagacgggga
    11401 attggcagtg gcaggtggaa aggttagcta taaaaattgg cattcatcat ctctggcagg
    11461 taaagtccgt gttattcatc attttgccgg tagaagttcg gtgaaagaaa aaaatgaaga
    11521 tttgatgaca gcaattaaaa atgccggttt tgatcgctcg aaataccaaa cggtgacgat
    11581 tgtaaatgct gatgatgcga ttgtcggcac aggggtattt gtgaaaaata gtgtggaaaa
    11641 cggcaaactt gacaatcctc acagccaagt agtgttagac cagcaaggca gtgtgaaaaa
    11701 agcgtggggc ttaaaagaga aagagagctt tattgcagtg ctggataaat cgggtaaggt
    11761 acaatttacc tctgagggca aactctctgc aaaacagatt caagaagtga ttgggttggt
    11821 gaaaacatta acgcaataac cagtgttttt atgggcggat tttgtatccg cccatcattc
    11881 tagataataa atcgataact tatcgtgtga tgatactctt ctcctgcttt tgttatcccg
    11941 ccaaattgga gtaattcagg ctggtttagt gaatttggga aaaactcagg ctctaatgct
    12001 acaccagcgt aatcttcata ttcgccttta gtcaaattag gttgtccttt tagccagttg
    12061 ccggtgtaaa gttgtaatgc tggcatggag gtgcggagtt ccatttttaa gtcttccacg
    12121 gctaaacaag cggtcggatt ttcactattt tttgcaagat gaaaggagtg atcgtaacct
    12181 tttaccgctt tttggtcgtc atcagccaat aaatcttgcc caattttttt agcattgcgg
    12241 aagtcaaaac tggtactctc cacttttttc agtggagcat taggaatacc gctggcatca
    12301 accggtaagt atccgtctgc attaattatc agctcgtgat ctaaaatcgt ctctgaacct
    12361 tgcaaattaa aataggcgtg attagttaag caaagcggtg tatcttggtt agcaacggca
    12421 tcaaagctga tttgaagctc attctctgtt aagcgatact ccacaaaagc tttcacttcg
    12481 ccgccaaatc cttcctcgcc atctgcaaaa aaataactaa atttgaccgc ttgttcgctt
    12541 tggctttcaa gttgccacac tattttatct gccccaagct cgccaccgtg gagattatgt
    12601 tcaccgttat ttttagcgag ctggtaggtt ttgccgttta agcgatattc ggcattggca
    12661 atgcggttag cgtaacgccc aatggtcgct ccaaagaaag cggtctgttt ttgccaattt
    12721 tttgcagacg tggtgactaa tacttcccgt tcctcgttcg gcattttgac aatgcacgaa
    12781 agccaagaag ccccaacatc agaaagggta atgcgtaaaa acgcattttc aagggtgaat
    12841 aatttcatcg gttgacctta attacctagc acggcatagc cgtactaggt taagcataat
    12901 tcagccctgc tagggctgag gttatttatt ttagagtagc ggagctactt ccttatgctt
    12961 aacccggtac gctttagcgt gccgggcatt ctcgcacccc atcggaggca gtacaaacgt
    13021 ggaaggtttc tttaatgccg gtttgctttt cgtaattgtt agcaacggct tggcggagat
    13081 cttccacttt gtcatttggc actaggcaca cgacacaacc gccaaagccg ccgccagtca
    13141 ttctggctcc gccgttttta ccgatagctt gttgggcgat ttcggctaag caatccactt
    13201 cagggcaggt gatttcaaaa tcatcacgca ttgagtcgtg agattgagcc atcaactccc
    13261 cgagtttgac catatcattc gcttttaagg cctctactgc ttccagaact cgttgatttt
    13321 cgctgatgat atgttttgct cgtttatacg ccagctcatt ctcttcacta agttctgagg
    13381 ctcgttcaat aaattgttcc gctgtgacat cacgcaattt tgctttgccg aagaagtggg
    13441 cggcaaattc gcactgttca cggcgactgt tgtattcgcc ggtaaccaag tcgtgtttta
    13501 cgttggagtt aataatcgca atcgaatagc cttgtggcac aggggttggg gtgatttcta
    13561 aactacggca gtcgatcatc actaaatgat cttgttgccc gagagcggag gtgagctgat
    13621 ccatattgcc gcagttgcag ccgacaaatt tattctcagc ctcttgcccg attaatgcga
    13681 tttgcggtaa agaaagcggt aaatcgccga gtaattgagc ggttttacca atcgacactt
    13741 ccaatgcggc ggaagaactt aaacctgatg acatcggcac atcgctggta atggctaaat
    13801 ctgctccttg tttgaattcg gggtgtttgg cttgaataaa tttcaccacg ccacgcacat
    13861 aattcgccca tttttgctcg cttggctcaa tcgcctggct taaatcaaat tcatcttgtt
    13921 ggttgagatc aatggcgaac acccgccatt tgttgtcact acgtttggca aagctaaccg
    13981 ctgtgccgta attgatggca caaggcatta caaacccatc gttgtaatca gtatgttcgc
    14041 cgataatatt cactctgccc ggggcaaata ccgtgcgttc tgctttataa ccaaaatgtt
    14101 gttcaaattg ttgcaaagcg atttcttgtg gtttcatttc aattccttat ttatctttat
    14161 agtgtttggt atcacttact gcatttaaac gttcggcagc ttgttccggg gttaaatcac
    14221 gttggctttc tgccatcatt tcataaccga ccataaattt acgcactgtt gccgaacgca
    14281 agagcggcgg atagaagtga gcgtgtaatt gccagtgagc attttccttg ccgttaaacg
    14341 gagcgaagtg aaagcccatt gaataaggaa agctgatgtt aaacagattg tcgtaacggg
    14401 tggtgagctt tttcaatgca agggctaaat ccgctttttc cgcttcgttt aaatcggtaa
    14461 tggatttgaa ctgcttggct ttcggcaaca gcaaggtttc aaacggccaa gccgcccagt
    14521 aaggcacaac cgcaatccaa tcttcggttt ctgccacaat tcgctcttgt ttgtctaatt
    14581 cacgttgtgc gtagtcaagc aacagtggcg agccatattg agcaaaatat ttcgcttggc
    14641 attcgtcttc aatcgcaatt tcattaggta gaaaattact tgcccaaatt tgcccgtgtg
    14701 ggtgagggtt agaacacccc atcattgagc ctttgttctc aaaaatttgc acccactgat
    14761 aatgctcttt cagctcattc gcttgctctt gccaaactgt taccacatca gtaatttcct
    14821 cgatggaaag ttgcggtaag gttttactat gatcaggcga aaaacagatc acacggcttt
    14881 cgccttgtgt atgagaaatc tgaaacagcg gatcatcatt cggtattggt gccggtgtgt
    14941 ctggtagcag ggcagagaag tcgtttttaa acacaaacgg cttggtgtaa ttcgggtttt
    15001 gttcgccggt aatccgctta ttgccggggc agaggtaaca acttgggtcg tagctcggtt
    15061 tttcatcttg cacgatttct tcttgttgcc cttgccacgg gcgttttgcc cgatgcggag
    15121 agactaaaat ccattgattt tttaatggat taaaacgacg atgtgggtga tcgttgagga
    15181 caaattcgct catataaaac tcctcgaaat tagttatgta aagagtatag cgaaagtcag
    15241 acaaaatgac tgtgacatag ctcacaaaat aatgatgtca agcggtcaaa tttcagaaaa
    15301 attttgcaag ttttcctcca ttttgcgatt taaggtagct tacgtggaat tgaatgtggc
    15361 ttttggtaga atagatgaat atgcaagata ttaagacaaa aatagatgga aagactgaat
    15421 aaatttgatg cttggttaat gaatcataaa cctaatgcta ggttaagcgg agcgagtcgt
    15481 ttgttgacag agttttggtt ctttgggtta aaagaagcta gagcctgttt atttgcggga
    15541 ttatttttta ttgcgatgtt ttcggtacca aaaacgggat tatggggctt acctcgttat
    15601 gatattttgc tgatttttgc catagtggtg caaattgcaa tggtgaaact ggggctggaa
    15661 acgtgggacg agctaaaagc aataagccta tttcatttgg ttggttttgt gttagaagtc
    15721 tttaaaacat cgagtaatat tcaatcttgg gcatatccgg attttgccta tactaaattg
    15781 ttcaatgtgc cattatttac tggttttatg tatgcagcag ttggttctta cattatccaa
    15841 gcgtggcggt tgtttgattt gaaaattaaa agccacctgc cctattggtt agccacttta
    15901 acggcactga tgatttacct gaatttcttt acgcatcatt acattgggga ttaccgttgg
    15961 tatattgccg cttttgcatt agggctgtat gcacgtactt ttgtattttt caccccttat
    16021 gacattacac gaaaaatgcc actgctgctt tctttcttcc tgataggttt tttcatttgg
    16081 ttggcggaaa atatcggtac gtttgccgga atttggcgct acccgaatca gtttggggct
    16141 tggtctatgg tgcatatgag caagtggagt gcttgggcgt tattggtgat gatgactttt
    16201 accatcattg tgcatttaaa gcatatcaag caaacagtca gagtctcgag cgattaacaa
    16261 gctgtgggtt ttggcgaaaa ttttactcat cagttatttt cagtggaatt aggggcaaat
    16321 aataatttgc ccctatatca aattatggat tttccccttc cacatcaata atcatcactt
    16381 ccgactgtga gcctaaccgc atcggcacgc cccagaatcc gtagccggag gtgacaaaat
    16441 aatgcccgaa gccgattttt tcatagccgt aatgtaatcg atagatcagt ttggtaataa
    16501 tacttgcagg gaacacttgc cctttgtgag cgtgtcctga aacttgaata tcaatcggca
    16561 atttagcatg ttgctcaatt tcagtcgggc ggtggtcaag caaaattacc ggtagtttag
    16621 tgttcacgtt ttgtaaaagc tgttcggcag aaggtcgatt tttgactaaa tcatcattac
    16681 gcccaataag ggtaaatttt ccgttaattt cggccgtttg atcccataaa acagtaatac
    16741 cggcgtcggt aatttctttt gcaatcgttt cttgagcccc aaaaaagtcg tgattgccaa
    16801 gggtagcata cacgccaaga ggagctttta atttggctaa atgaggctgc attttatcgg
    16861 caacataaac ttcggtattg tcgtccataa tatcgcccgg caataaaatc agatcgactt
    16921 tttgctggtt gaaaatctct gccagttggt caagttgctt ggttccgaac agttttccta
    16981 aatgcagatc gcttgccata ccaatgcgaa gcggtttctc taatggttta tccaatttca
    17041 ccgaataatg tactactgtt ggggtataag cgttgtaata gccaagccca agtaagccaa
    17101 agaatgcaaa tgggtaggcg attttcagcc agcgattgac ggtagtattg ccgttgaata
    17161 ttttgtaaat acaagcggtg aaaaaactga taaaaaatgc aaaaagtaac aataccaata
    17221 tgaatgccat tattctaaat agcgcaaaag tacgagtaag atgcaaaatc actaatccgt
    17281 ttgcgactaa gaaagtgaga ataccaactg ttttacggtt tttacttgat aaattaaata
    17341 accagctgac agtctttgca aagttgaata aaaagagttg taacaccgca atcacaaggg
    17401 cggtggtaat gtagtaacga tattccatat ttttccttca ataaattggc taaacccagc
    17461 cgatttgtct taataaaaat aagccgatgc tggcactaaa cattgagccg agagtcgtaa
    17521 tgccgatcag atttgcggct gcctcgccgt ctccgccata gttgcgtgtc attgcgtagg
    17581 tggctgaagc aacgggagca gaagcagcca caaagacaat gcctagttcc atcggcggaa
    17641 gttgaaacac aaatttcccg agtagtagca agaaaattgg agcaaaaatc agccgaatcg
    17701 aggcagaaaa cagcacaatt ttattgatgg tgctttccac tccttgatta aattgtttca
    17761 actgtttgaa attaatgctt gctccggtgc agataagtgc taatggtagt gtcaggctcg
    17821 ccattaattt accagcagac tcaagcgggg ttggaatttg gtggcttaat tcaaaatagt
    17881 taaaagttaa gcctgcaatg acggctagaa tcagcggatt tctcaatatc gacaatatta
    17941 atctgccaaa attcactttt ttctcgctta acgatgaagt gagggtaatc actgcaagca
    18001 cattaaaggt aatcaccaag cacgaaatat aaactgaagc ggcagcgaga gctttatcgc
    18061 cataagcatt gagaattaag gcaatgccta aaatcgccgc attggtacgg aaagtgcctt
    18121 gagtgaaaat acaacgataa cggcggtttt caatatgtcg agccgcccac cattccgccc
    18181 ataaatagat caaaatcgaa ccgccgactc cggtcgagat caggttgatt tggctggaga
    18241 aatctaacgg gctttttagc atattcaaga aaatcatcgg tggcagggta aaattaaata
    18301 ccacctttga tgccatattg caaaaggtat cgtcaataaa ttttcgtctg cgtggaaaaa
    18361 taccgaacag cattaacaaa atggttggta aggtaacgcc aatgctaaat tggatagagt
    18421 cgaaaagcat aatgtttgcc ttaaatgatg gcacgagtgt cttttgcttg taccgtagat
    18481 tatcaataaa gtaccagcgt ggacgctggt actatcatca atggaaatga aattacaatc
    18541 ctgcctttaa gcttgcttcg acaaatttat ccaaatcacc gtccagcact tgttgggtgg
    18601 tgcgatgttc cacaccggta cggtggtctt taatccgtga atcgtctagc acataagagc
    18661 ggatctggct gccccagccg atgtcggatt tgttgtcttc catcgcctgt ttttccgcat
    18721 ttttcttgct catttccagc tcatatagct tcgctttaag ctgtttcatc gcctgatctt
    18781 tattttgatg ttgtgaacga ccgttctggc actgcaccac aatgccgctc ggtatatggg
    18841 taattcgcac cgccgattcg gttttattga cgtgctgacc acccgcccct gaggcacgat
    18901 acacatcaat ccgcaaatcc gcagggttga tttcaatatc aatatcgtca tcgacttccg
    18961 gataaacgaa agccgccgag aacgaggtat gacgaccgtt attggcatca aacgggcttt
    19021 tacgcaccaa gcggtgaata cccgtttcgg ttctgaacca gccgtaagca tagtcgccgg
    19081 aaattttcac ggtggcggat ttaatccctg ccacatcacc gtctgaggct tccattagtt
    19141 cggctttaaa ccctttgctc tctgcccaac gcaaatacat acgcagtaac atttctgtcc
    19201 agtcctgagc ttccgttcca cccgaaccgg cttgcaaatc caaatagcaa tcggcactgt
    19261 cgtgtgggcc gctgaacatc cgcttaaatt ctaaatcggc aagttttttc tctaattcgt
    19321 ctgcttccac ttgggcttca ttgaaggtat cctgatcttc tgcttccacc gccagctcaa
    19381 tcaacccttc aacatcttca atcccttgct ctaaggcttt aatcgtattg acgatctgct
    19441 ccaacattgc ccgctcttta ccaagcgctt gtgctttttc cggcttgttc catacatcgg
    19501 gttgttccag ctcggcattg acctcttcta agcgttcgac tttatgctca aagtcaaaga
    19561 taccccctga tcaccgcggt gcgctcgctt aaatcagtta gttggttttt aattgggttt
    19621 aattcaaaca ttgtcgttcc tgtttttgct tattttaaaa agatgaatta tagctgaagt
    19681 taagcaagcg gtcgaatttt gcaagaaatg gagcaatgat cgtttctagt aatgagattt
    19741 ggttcataaa agttgcaact gtttctgcta tttggctttg atagctaaaa atctttaaaa
    19801 aaacaagcgg tcgttttacc ccattttttg caaaacgacc gtagcttctt gctataatcg
    19861 ctgaaaaaat gctgcaagga gattttatgg caaagcgccc aagtgatatt gatgcaaccc
    19921 tattgcaact ggaaatccta cgccgtatcc ccaaattacc cgctaaaatt gatgctaaaa
    19981 gtttgcacca acagctatta gatgccggct ttgaacggga tattcgcact gttcagcgtt
    20041 cgctcaaaat gttatgcgag cattttgata ttgaatgcga tgagcggagc aaaccctttg
    20101 gttatagctg gaaaatccgc tcagaggggc tggcattgcc gattctcaac gaacaacaat
    20161 cattactgct aaaacttgcc gagcaacagc tgaaatatct gttacccgca aatttaatgg
    20221 catcaatgaa accctttttt gagcaggcag acagaatggt ttccggggcg aaagacaagc
    20281 ccgaatacca atggctaaat aaagtttgct ccacgccaac cagcctaccg ctgatccctg
    20341 caaaaattaa agaagagatt tttaattccg taagccaagc cttatttcaa aataagttgt
    20401 taaagatcga atatcagaac cagcacggca aaaaacacca agcgacaatt atgccgttgg
    20461 cgatttcaca acaaggtgcg agcatttatt tggtctgccg ttacgaaggg tttgacgaca
    20521 accgcctgtt ggctcttcac cgcattcgta aagcggagat ttctactttt agttttgaac
    20581 gcccgaaaga ttttaatttg caagcctacc aagatgccgg acacctcggt tttggcaatg
    20641 gcgaaaaagc tagccttacc ttttcgatta gcagagacgc cggtttccat ttaaccgaaa
    20701 caccgcttgc caaagatcaa aaaattctcg aggagagtga aactcactac cgtattcaag
    20761 ccatagtcgc tgataatgat atgctaacct ggtagctacg caaatttggc gaagatattt
    20821 gggatatcga gaaagaagcg ctaaaataag ctaaacaagc ggtcggtttt gtgggaattt
    20881 ttgctaaata gtatcgaccg ctcctcccta aacagacctt tatcataaat acaactcttt
    20941 caatcagata ttcttactgt ctattgatga acgattcgaa tttttctagt ggttgaaatt
    21001 taaactaatt ttcttgcttt ttggcatttt tcagactttt aattctctcc attagcctgt
    21061 ttttttacgc tctttttcat aaacaggaat aaccaatgcc ttttcaactt actttctgcc
    21121 aacaagccgg caatcagcac cagaaaaatc aagatgcact gtttaacggc aaagcggtgt
    21181 accaatggca acttaaaaat gccgagagtt atctgctgga taacgacaaa gtaatttttg
    21241 gggttgcgga cggcgtttcc agccaccctc gaaatcatct tgcctcacat tatttaatgg
    21301 cgttactcag ccaatgccaa tcccttgata gccaatggtt gcgacaggct cacggcgatt
    21361 tttgcgaaaa attagcgaaa gattattttg gtgcttcttg cacttttgtt gcttgtgaat
    21421 tacaccataa cggcagaggg cgtatggtta atgtgggcga cagtcgagcc tataaaatta
    21481 cggcaaacgg ggagtggcaa caactgagtt ttgatcacac catactggct gaaatgaaac
    21541 agaaaggctt ggttaaggaa gggagcaact atgcctcaat gtataacggc ttaaccgatt
    21601 gtattgttgc cgattttgac gccgatgatt tccgcattca tatagccgaa tttcaactgg
    21661 aaaaaggcga gagcattttg ctctgttctg acggtttgca cgaacaactc gccccagaag
    21721 ctattcaggc gatttaggac ggatacgaaa gcccgtctca tcgtctgcaa atttgcagaa
    21781 aaatggtaaa aatgcaccgc ttgtatgacg atttttctgc ggtgatttgt gaaattgtgt
    21841 aattaaaacg acattatttg tcgcattggt gcttaaaatc cgcataaaac agaggagaaa
    21901 aaaatgaaaa acgatttcca ttatgccgct gaattaatcc gcaatgcaga cggcatttta
    21961 attaccgcag gggctggaat gtcggtggac agtggcttgc ccgatttccg tagcgtggga
    22021 gggttatgga attcctaccc accgctcaaa aaatttaatt tgcaatttat gcagattgcc
    22081 acaccgcttg cctatcagga acgccctga
//
LOCUS       DANLZC010000025        22099 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.025, whole genome shotgun sequence.
ACCESSION   DANLZC010000025 DANLZC010000000
VERSION     DANLZC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22099
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.025"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            43..1065
                     /locus_tag="PXE44_000755"
     CDS             43..1065
                     /locus_tag="PXE44_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5588290.1"
                     /translation="MANGTSRNRRSSGRVTLADVAKVVGVGQMTVSRALRTPEMVSEP
                     LRKRIEEAIIELGYIPHFSARQLASAESHNLVIVTTSISSTENTLILSALRKSVADSA
                     LQMVILIADQESWFQELINHAPQAIVFLNLDCPPNVREWIKNSNIPTVEIGAIKKEPI
                     HLSVGIDSHEAMEKMIHFLVKKGYREIGLLSAKQDLSIFKQYLESWHTTLFAHHISPL
                     KIHHVVEDISFSAGATLLTEALLNWEAIDALVFLSDELACGALYEALRRHMSIPKDLA
                     IAGLGGLEVGSISYPKLTTIAIPYEQMGKIAGKQLLTLLSGKTLSAEEMIVKLPTKLV
                     IRDSAA"
     gene            1178..1591
                     /gene="secE"
                     /locus_tag="PXE44_000756"
     CDS             1178..1591
                     /gene="secE"
                     /locus_tag="PXE44_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL5588291.1"
                     /translation="MATEIKTKTPEQVEQKGGKSKGANTALWLVAIVFLAVAAVGNAY
                     FSSHFSLIVRVLLLVVLLVGAVALAAITNQGQKAIHFIKESRTELRKIIWPTRPEATQ
                     TTLIVLAMCVVVSLVLWGIDSIIVALITFVTSLRF"
     gene            1593..2156
                     /gene="nusG"
                     /locus_tag="PXE44_000757"
     CDS             1593..2156
                     /gene="nusG"
                     /locus_tag="PXE44_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL5588292.1"
                     /translation="MSEVEVKENGSKRWYVLQAFSGFENRVAVTLREYIKLHKMEEQF
                     GEVLVPTEEVVENVGGKRRKTERKFFPGYVLVEMEMNDDTWHLVKSVPRVMGFIGGTA
                     DRPAPITKREADRILNRVQETAEKPRHRKEFQPGESILVTEGPFADFSGTVEEVDYEK
                     GRLKVSVSIFGRATPVELEFSQVEKQS"
     gene            2389..2817
                     /gene="rplK"
                     /locus_tag="PXE44_000758"
     CDS             2389..2817
                     /gene="rplK"
                     /locus_tag="PXE44_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL5588293.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     RTESLEKGLPIPVVITVYADKSFTFVTKTPPAAVLLKKAVGIKSGSGKPNKDKVGTVT
                     QEQIRQIAETKAADMTGATIETKMKSIAGTARSMGLIVEE"
     gene            2822..3511
                     /gene="rplA"
                     /locus_tag="PXE44_000759"
     CDS             2822..3511
                     /gene="rplA"
                     /locus_tag="PXE44_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL5588294.1"
                     /translation="MAKLTKKMKAIKAGVDSTKAYDINEAVEVLKKFATAKFVESVDV
                     AVNLGIDSRKSDQNVRGATVLPHGTGRTARVAVFTQGANAEAAKAAGADLVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKSGQ
                     IRYRNDKNGIIHTTIGKADFSAEQLKDNLNALLAALTKAKPTTAKGIFIKKVSISTTM
                     GAGVAVDQASL"
     gene            3573..3992
                     /locus_tag="PXE44_000760"
     CDS             3573..3992
                     /locus_tag="PXE44_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588295.1"
                     /translation="MLRLLSFCFIFLLSACTVLPQIKQLPPQVESVKSFKVENNGQQS
                     LLLIQFYENKWRWIQTDPLGAPIARVWLTKKGWEPDGFVMPNKQAQLLFTALVNALNP
                     HSPPFYLDKGWKIEREKSQFTIWLPDSSKWKVEILGE"
     gene            4079..5301
                     /locus_tag="PXE44_000761"
                     /pseudo
     CDS             4079..5301
                     /locus_tag="PXE44_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            5294..5698
                     /locus_tag="PXE44_000762"
     CDS             5294..5698
                     /locus_tag="PXE44_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL5588296.1"
                     /translation="MHKFTFPIEQIAPLVPHSGNMILLDRITAFGEDFLTAETNIRPD
                     NPLIKHGKLATYASIEIMAQAGEPVRLGYLLGTRKLHLHRQEIPIGSQLQIQIKMSIQ
                     DATGFGVFDCQLIDLADNQVLLEGALNVFSPK"
     gene            5808..7241
                     /locus_tag="PXE44_000763"
     CDS             5808..7241
                     /locus_tag="PXE44_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL5588297.1"
                     /translation="MQEAYDIQHLCTRKEDKSFDRKSARKEPKEILRHLIGFANADGG
                     LLVIGIEDNGEITGFNYQKAHQPEEFIHALHNLQKMPIPVTYQQIEVVNSRGLADFVL
                     LFEVTASSGRVITSHDDRVFLRSKDQTLELNFEQRLQLEYEKGQRAFEEQPIARADFT
                     DLDMDLLEQYRQKLHTDRSIEEILKARDLINKEGQITNACILLFGNNPTRYFPNARLR
                     FLRYEGKAPLTGRTFNVVKEFMLEEPIPKLLESAKAVLKSQLREFQRLDENGQFTKID
                     EYPEFAWLEGIVNAIAHRSYSNQGDCIRISMFDDRLEIFSPGGLPRPVTLENMYYTRF
                     SRNPKIARVLYEFGWVKELNEGVNRMAEEMNAYQLNTPSYFEPNENAVRLILENNIEH
                     RTLRALERIEEIFTQDIMATLNKNEVTILQYLYENEMITVKIASEILDRSKELSRRIL
                     KSLEQKNILTWHGLSPKDPTQYYTLKK"
     gene            7255..7983
                     /gene="fabG"
                     /locus_tag="PXE44_000764"
     CDS             7255..7983
                     /gene="fabG"
                     /locus_tag="PXE44_000764"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL5588298.1"
                     /translation="MNNTVLITGSSRGIGKAIALTLAQQGFDIVVHCRSRVEEAEAVA
                     QAVRNLGKNARVLQFDVADREKVRSILEADVEQHGAYYGVVLNAGLTRDNAFPALTDE
                     DWDSVLRTNLDGFYNVLHPIMMPMIRRRKAGRIVCITSVSGLIGNRGQVNYSASKAGI
                     IGAAKALAIELAKRKITVNCVAPGLIDTDILDENVPIEEILKMIPAGRMGDPDEVAHA
                     VAFLMDEKATYITRQVIAVNGGLC"
     gene            8046..9191
                     /locus_tag="PXE44_000765"
     CDS             8046..9191
                     /locus_tag="PXE44_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDL5588299.1"
                     /translation="MKRVVITGIGVVTAFGKEWAEIHGYQPPKHWNRKQLRSLGRVSQ
                     FVVEASERALAQAGLLDENGVIFDWLKDRRMGVACGSSTGSTNDIKDAAELLMTGKSD
                     GFNANTYVRMMPHTTAANIGIFFGLTGRIIPTSSACSSGSQGIGYAYEAIKYGMIPMM
                     LAGGGEEFCASKVYVFDSLYAASRRNDNPKGTPRPYDKDRDGLVIGEGAGMFVLEELE
                     HALARGANIIAEVVGYGANSDGSHVTRPQATTMQRCMELALKDAGIQPNQVGYVNGHG
                     TATEQGDIAETQATTAVFGKIPLSSQKSYLGHTLGACGALESWFSIEMMRDGWFAPTL
                     NLENVDERCGDLDYLQGDGREIHTDYVMNNNFAFGGVNTSLIFKRWK"
     gene            9205..10110
                     /locus_tag="PXE44_000766"
                     /pseudo
     CDS             9205..10110
                     /locus_tag="PXE44_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dialkylresorcinol condensing enzyme"
     gene            10133..11275
                     /locus_tag="PXE44_000767"
     CDS             10133..11275
                     /locus_tag="PXE44_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDL5588300.1"
                     /translation="MLSNVYITNTSAFLPNAPVSNDAMEAVLGMVGETPSRVRKMILR
                     SNGIQHRYYAIDPITRQATHSSTELAVQAVKKLGNSATSATCLAVGTSYPDQIMPGQG
                     VMVHGLLDAEPMEVLTTAGVCVAGMAAMKHAYNAVRIGEHRAAIATASETASAIMRSE
                     HFRAEIDHKKLDDARPEIGFEKDFLRWMLSDGAGAVLLANQPNEKGLSLKIHWIDLVS
                     YANEMPVCMYAGAEFIDGNYTGWKAMSEAQRAECSAMAVKQDVKLLNENIVKYTVEKA
                     LERIIPKYKLNAEEIDYFLPHYSSGSFRDKLLEGLQRINFLIPQEKWFTNLTTKGNTG
                     SASIYIMLDEFIRTHDLKQGQKILCYVPESGRFSSCFMLLEAVVNS"
     gene            11324..11539
                     /locus_tag="PXE44_000768"
     CDS             11324..11539
                     /locus_tag="PXE44_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL5588301.1"
                     /translation="MDINDIPQDNSKIFNGERKVIYARNPQPEIRQMISSILACYAWD
                     ENNPFVAKSDARLKALAEVVGKAEIGD"
     gene            11820..12311
                     /gene="rplJ"
                     /locus_tag="PXE44_000769"
     CDS             11820..12311
                     /gene="rplJ"
                     /locus_tag="PXE44_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL5588302.1"
                     /translation="MALNLQDKQAIVAEVNEAAKGALSAVVADSRGVTVEKMTELRKA
                     AREAGVTMQVVRNTLLRRAVEGTEFECLTDTFTGPTLIAFSNEHPGAAARLFTEFAKT
                     NKEFELKGAAFEGKKQDVAFLATLPTYEEAIARLMGTMKEAAAGKLVRTLAALRDQME
                     AAA"
     gene            12370..12738
                     /gene="rplL"
                     /locus_tag="PXE44_000770"
     CDS             12370..12738
                     /gene="rplL"
                     /locus_tag="PXE44_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL5588303.1"
                     /translation="MSLTNEQIIEAIASKSVSEIVELITAMEEKFGVSAAAAVAAAPA
                     AGAAAAEEKTEFDVVLAEAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGISK
                     AEAEALKKELEEAGAKVEIK"
     gene            complement(12845..13675)
                     /locus_tag="PXE44_000771"
     CDS             complement(12845..13675)
                     /locus_tag="PXE44_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL5588304.1"
                     /translation="MVQKPLNHYLEMQEHFLYVPYLHHHRRIRVLLPKDYHNEGWERY
                     PVLYMHDGQNVFYSKESYSGHSWKIIPTIKSHQELPKMIIVGIDNAGANRLNEYGPWK
                     TTTGSTPETRNAGGLGEEYAKWVVETVKPFIDIHYRTKPQQEHTLLAGSSMGGIITAY
                     MGSRYPHIFGHLGVFSLASWFSEPDFLRFTHQYPLQPNTKVFIQVGTNEGDEIDSHFI
                     SNTNQTYIDCSLNYYQALIRIGVPLDNIRLRIMANEIHHEMHWADHFVEFLHFSLLRK
                     "
     gene            13901..15442
                     /locus_tag="PXE44_000772"
     CDS             13901..15442
                     /locus_tag="PXE44_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDL5588305.1"
                     /translation="MQASLQDILEVVKAKNLTYQQKLMSLGNIAERLFNPIELLGYTE
                     EEWEHIENQMICDLNEGYAIYRPRYILPDYNVYMQKGCQFLDLPPPTNLDEALDGLLI
                     LYSHVPSITTFPVYIGRLDQLLEPFITDEEQDYIKIKRFLNHIDKTISDSFCHANIGP
                     NDTKAGRLILKAVIELENITPNMTIRYDKTKTSREFAELAAKACLLVSKPSFANDAYY
                     IQDLGEEYGIASCYNALPECGGAYTLTRLRLGTIGRACASVEQMVNEMLPKVAKLALS
                     TMDKRHKFLVEESNFFESSFLVQEGFIKRTNFTSMIAIVGLADAVNHLLKQEGLNETF
                     GQSKRGDEIATLIMDKLQAVNDAHKGLYVERTNNQYLLHAQVGANNHAEDKMNTPAHR
                     IRVGEEPTLLAHLKQSAPFHKYFPSGTGDLFAFDQTYTDHLDAVVDIIDGSFAQGYRY
                     ITTYLKNTDLIRVTGYLVKKSEVERYRKGEAVLRDTTWYGSGTDDCAKVFDRQLRDEE
                     NVNAG"
     gene            15445..16233
                     /locus_tag="PXE44_000773"
     CDS             15445..16233
                     /locus_tag="PXE44_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HDL5588306.1"
                     /translation="MAALSEIFVPLHRIIPFSNVEGQGNRTSIFLQGCKLNCLYCHNP
                     ETIPRYSKEAKQVSLQYLYDQVMEAVPFIRGVTISGGEPTIHHKKLVPLFEALREQGL
                     TCYLDSSGFFDFEATEPLINVTDKFLFDLKGDGAGLQTLCFDRKNQAGKVPEQALPNV
                     NHIKPENLARNLQNLAKLLPLDKVEEVRLVYLNEFYNAHHLLEKVADLLKPYPDVLLK
                     IIRVHTKGARDETGLARFVPTIEQTDELAAFAKRHGIQKVITIY"
     gene            16246..17503
                     /locus_tag="PXE44_000774"
                     /pseudo
     CDS             16246..17503
                     /locus_tag="PXE44_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(17582..19591)
                     /locus_tag="PXE44_000775"
     CDS             complement(17582..19591)
                     /locus_tag="PXE44_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5588307.1"
                     /translation="MSAENMPSVIRFEQAVAKKDYESACTELLSILSKLDSNFGGISN
                     IELNMPEQIENLENDKAIYFCTRMAVAITRLFEDPALEISEHGAMRFLTLQRWIALIF
                     ASSPYVNADHILRTYNRNKESANPNTVDLDATLQALIKFCILYLPESNILLNLDAAWN
                     ASSDLTASLCFALQSPRFIGTSSAFAKRAAILQWFPEKLAQIENLNKLPSAISHDVYM
                     HCSYDIEANKHNVKRSLNAVIRRHLLSVGWEDRKIEQLGTRNNKPVMVVLLEHFHSSH
                     SIYRTHSTSMVAAREHFHLIGLGSDAVDEMGQQVFDEFHLLPQDGSLFDRLSFLKDIC
                     DKNNPAVFYMPSIGMDLTTIFASNTRLAPIQAVALGHPATTHSDFIEYVIVEDDYVGS
                     ESCFSEQLLRLPKDALPYVPSALAPQNVVYNLRENPEVIHIGIASTTMKLNPYFLEAL
                     KAIRDRAKVKTHFHFALGQSSGITHPYVERFIKSYLGNDATAHPHSPYDEYLNILHNC
                     DMMLNPFPFGNTNGIIDMVTLGLVGVCKTGPEVHEHIDEGLFKRLGLSNWLITQTAEE
                     YVTQAIRLAENHEERLAIRRDIIENNKLQTLFSGDPRPMGQIFLAKVQAWLADKNSKN
                     AEVEVKTKKVRKAATASQSAKKQTTSKTQTAKAEKDNTAKTETKS"
     gene            complement(19601..20938)
                     /gene="rimO"
                     /locus_tag="PXE44_000776"
     CDS             complement(19601..20938)
                     /gene="rimO"
                     /locus_tag="PXE44_000776"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDL5588308.1"
                     /translation="MSASPNIGFISLGCPKNLVDSERILTELRADGYNIIPSYENADL
                     VIVNTCGFIDSAVQESLESIGEALEANGKVIVTGCLGAKENQIREVHPKVLEITGPHS
                     YEAVMNHVHKYVPKPEFNPYTSLVPKQGIKLTPKHYAYLKISEGCDHRCTFCIIPSMR
                     GDLASRSIVQVLDEAKRLVDAGVKELLIVSQDTSAYSLDRKKEEATKTVFWNGMPIKS
                     NLITLCEQLGSMGVWVRLHYVYPYPHVDDLIPLMAAGKILPYLDIPLQHASPKILKAM
                     KRPGSIERTLERIKKWREICPELTLRSTFIVGFPGETEEDFQLLLDFLKEAQLDRVGC
                     FKFSPVEGAVATDMPDQVPEEVKEERFHRFMQLQQEISAARLQAKIGKTVQVIIDDID
                     EEGIIGRSMAYAPEIDGVVYVDNPSNQLVTVGQIISVTITHADEYDLWGILNN"
     gene            complement(21093..21785)
                     /gene="mtnN"
                     /locus_tag="PXE44_000777"
     CDS             complement(21093..21785)
                     /gene="mtnN"
                     /locus_tag="PXE44_000777"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL5588309.1"
                     /translation="MKIGIIGAMAQEIEILRQVMVEPKVTELAGCKIFEGKINNTRVA
                     LLQSGIGKVAAAIGTTLLIQLTQPDMIINTGSAGGLDPDLNVGDILISTEVRYHDVDV
                     TAFGYEIGQLPANPPTFSPNEQLVELAQKEAKKAGYNVVSGLICSGDAFINGNDKINQ
                     IRQNFPNVVAVEMEAAAIAQVCHGFNLPFVIVRAVSDVADKESHLSFEEFLPLAAEKS
                     STIVLAMLNNID"
     gene            complement(21843..22064)
                     /locus_tag="PXE44_000778"
     CDS             complement(21843..22064)
                     /locus_tag="PXE44_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HDL5588310.1"
                     /translation="MDNNYTLSAKITELETKVAFQELIIEELNQALIEQQFAIDKLQT
                     QIRHIAEKLKGAQLSQVASQAEETPPPHY"
BASE COUNT         6482 a         4472 c         4933 g         6212 t
ORIGIN      
        1 taaggattta gccctataat atggaacaat taggaaaata gtatggcaaa tggaacaagt
       61 agaaatcgaa gatcttcggg cagagtcact ttagctgatg tagccaaagt tgttggtgtt
      121 gggcagatga cggtatctcg tgctttgcgt acaccagaaa tggtgtcgga gccactacgt
      181 aaacggattg aagaggcaat cattgaacta ggatatattc cgcatttttc tgctcggcaa
      241 ttagcttctg cagaatcaca taatttggtc attgtgacga cgtcgattag ctcaacggaa
      301 aataccttaa ttttatcggc attacgcaaa agcgtggcgg attccgcatt gcaaatggtg
      361 attttgatcg ctgatcaaga atcttggttt caagaactga ttaatcatgc tcctcaagcg
      421 atagtgtttt tgaatttaga ttgccctcct aatgtccgtg agtggattaa aaacagcaat
      481 atccctaccg tagaaatagg tgcgataaaa aaagaaccga ttcatctcag tgttggaatt
      541 gatagccacg aagcaatgga gaaaatgatc cattttttgg tgaaaaaagg ctatcgagag
      601 attggattgt tatcggcgaa gcaagattta tccatcttca aacaatattt agaaagttgg
      661 catacgactt tatttgcaca tcatatttct cctttaaaaa tccaccacgt agtagaggat
      721 atttcgtttt cggcaggagc aactttgttg actgaagccc ttctaaattg ggaagcaata
      781 gatgcattgg tttttttatc cgatgaattg gcttgcggag cattatatga agcattacgc
      841 cgacatatga gcattccaaa agatcttgcg attgccggtt taggtggttt agaagtgggg
      901 agtatttcct accctaaatt gacaaccatt gcgatccctt acgagcaaat gggaaaaata
      961 gcaggtaaac aactacttac tcttttgagt ggtaaaacct tatcagcaga agagatgatt
     1021 gttaaattac cgactaagct agtgattcgt gatagcgccg cataatattg attttgcata
     1081 aaaatctata aaagatcgtg gcaagtcttg tagaaaatga tagaatttaa cgctttaaaa
     1141 taatttctga tttttttatt cattttttag gttatatatg gctactgaga ttaaaacaaa
     1201 aacccccgag caagtcgaac aaaaaggggg gaaaagcaaa ggtgcaaata cagcattatg
     1261 gctggttgcg attgtatttt tagcagttgc tgcggtaggt aatgcctatt tttcatctca
     1321 tttttcatta attgttcgag tgcttttatt ggtggtgttg ctagttggtg ctgttgcact
     1381 agcggcaatt acaaatcagg ggcaaaaggc aattcatttt attaaagagt ctcgaaccga
     1441 actacgcaaa attatctggc cgacacgccc tgaagcaaca caaaccacct tgattgtatt
     1501 ggcaatgtgc gtagtagttt ccttggtttt atggggtatt gactcaatca tcgtcgcatt
     1561 aattacattt gtaacaagtt taaggttcta aaatgagtga agtagaagtg aaagaaaatg
     1621 gctcaaaacg ctggtatgta ttacaagcgt tttcaggctt tgaaaaccgt gtggcagtaa
     1681 cattgcgtga atacattaag cttcacaaaa tggaagagca gtttggtgaa gtattagtgc
     1741 caaccgaaga agtcgttgaa aatgtgggcg gtaaacgccg taaaaccgag cgtaaattct
     1801 tcccaggtta tgtattggtt gaaatggaaa tgaatgacga tacttggcat ttagtgaaaa
     1861 gtgtgcctcg tgtaatgggc tttattggtg gtacggcaga tcgccccgca ccaattacca
     1921 aacgtgaagc agaccgcatt ctaaaccgtg tacaagaaac agcggaaaaa ccacgccaca
     1981 gaaaagaatt ccaaccgggc gaaagcattt tggttacaga aggtccgttt gcagatttct
     2041 ccggtacggt cgaagaagtg gattacgaaa aaggtcgctt aaaagtatcg gtgtcgattt
     2101 ttggtcgtgc gacaccggta gaattagaat tcagtcaagt ggaaaagcag agctaatttc
     2161 aattaatttc acacacaagc ggttattttt ctcaattttt tgcaaaattc agcggaaaaa
     2221 agaccgcttg taacaagatt tattcaacaa agcgaaagtt ttgcttgaaa aatagtcgca
     2281 aattctttaa aatttgcgac ctattacgag ccagcctgct gactcgtatt ttttatgtaa
     2341 caggggagct gattttcagc gttatcaccc atttagagga aaattaaaat ggcaaaaaaa
     2401 gtccaagcct acgttaaact gcaagttgca gcgggtatgg ctaacccatc accaccggtt
     2461 ggtcctgcat taggtcaaca aggtgttaac attatggaat tctgtaaagc attcaacgct
     2521 cgtactgaga gcttagagaa aggtttaccg attccggttg ttatcacagt ttacgctgat
     2581 aaatctttca cattcgttac caaaactcca ccggcggcag tgctactgaa aaaagcggta
     2641 ggtatcaaat ctggttctgg taaaccgaac aaagataaag tgggtacagt aactcaagag
     2701 caaatccgtc aaatcgctga aactaaagca gcagatatga ctggtgcgac tatcgaaacc
     2761 aaaatgaaat caatcgctgg tacagctcgc tcaatgggct taatcgtaga ggaataatac
     2821 aatggctaaa ttgactaaaa aaatgaaagc aattaaagct ggcgtagatt ctactaaagc
     2881 ttatgacatc aacgaagcag ttgaagtgtt aaaaaaattc gcaaccgcta aattcgttga
     2941 gagcgtggac gttgctgtaa acttaggtat cgattctcgt aaatcagacc aaaacgtgcg
     3001 tggtgcaacc gtattaccac acggtacagg ccgtaccgct cgtgttgctg tgtttacaca
     3061 aggtgcaaac gcagaagcag caaaagcagc gggtgctgac ttagtgggta tggaagatct
     3121 tgcagagcaa atcaagaaag gcgaaatgaa ctttgatgtt gttatcgctt ctccagatgc
     3181 aatgcgtgtt gtaggtcaat taggtcaagt attaggtcca cgcggcttaa tgccaaaccc
     3241 gaaagtaggt acagtaacac caaacgttgc agaagcggtt aaaaatgcga aatctggtca
     3301 gatccgctac cgtaacgaca aaaacggtat tatccacact actatcggta aagcagactt
     3361 ctcagctgaa caattaaaag ataacttaaa cgcattgtta gctgctttaa caaaagcaaa
     3421 accaacaaca gcaaaaggta tcttcatcaa gaaagtaagc atctctacta caatgggtgc
     3481 tggtgttgcg gttgatcaag catcacttta atttttaata gaatcaaaag accgtagtgt
     3541 taatactcta cggtcttttt ctttaggtac gaatgttaag attattatct ttttgcttta
     3601 tttttttgct tagtgcttgt actgtattac ctcaaattaa gcaattaccg ccacaggttg
     3661 aatctgtaaa atcctttaaa gttgaaaata atggccagca aagtttgctt ctaattcaat
     3721 tttatgaaaa taaatggcgt tggattcaaa ctgatccttt aggtgctcct attgccagag
     3781 tatggcttac taaaaaaggt tgggagccag atggctttgt aatgccaaat aagcaggcac
     3841 agctattatt tacagcttta gtcaatgcat taaaccctca ttctccacct ttttatttag
     3901 ataaaggttg gaaaattgag agggaaaaat cccaatttac gatttggcta ccagatagca
     3961 gtaaatggaa agtggaaata ttgggggaat aagcgaaaac ttgaacctct atgcttgaat
     4021 cacttgaata tgaattttat aattttagtg ccattacaca ataatactgg aataaataat
     4081 aatgacacta tatttatcag agcccgccat caccagtccg attggatatg gcatcgtgga
     4141 gcatttgaat gttttaatta atgcgaaagt atctccttta gatacgactt cacagcctta
     4201 tttacgacat aatatttcaa atagttcacc gaaatttcta gggcaagtct ctgctagatt
     4261 gcgtgatttt cctgttgaac tatctattga acaccgcagc cgcaataacc aactgctatg
     4321 gcacgccctt gcagaaattg agccgaaaat taccgcttgt atcgagcaat tcgggcgtga
     4381 gcggattgcg gtgatcatcg gcacgtccac taccggcgcc gatgaaaata ttcccgtctt
     4441 caaagccggt gtgcagggcg attatgccac cgagcagttt aatcaacagc aaattctctt
     4501 ctctgcgccg tctgatttcg ttgccgaagt gtatggctta acggggctga gttacggcat
     4561 ttccaccgcc tgcacctcag aggcaagagc gttaatttct gctgcccgtt tactgaaagc
     4621 cggcttgtgc gatgcggtga tttgcggtgg tgtggatacc ctttcgcctt taactatcag
     4681 cggatttagc tcgctggaag tgttatcaaa ccaacaaacc aacccatttt ccgctaaccg
     4741 taacggcatt aacatcggcg aaggcgcagc ggtgtttgtg atgagccgtg aaccactgga
     4801 cgaacatcgc attcagttac tgggttatgg ttccagctcg gacgcttacc atatgtcctc
     4861 acctcacccc gaaggggaag gggcaatttc cgctttccaa aatgccctgc acaatgctga
     4921 tttagacgca gccgacatcg gctggatcaa cctgcacggc acagggacgc ttcataacga
     4981 tcaaatggaa agcctcgctg tggcaaaaat cttcggtaat cagaccgctt gcaccaccac
     5041 caaaccctac accgggcata cccttgggct gccggtgcgg tggaagcggc aattttgtgg
     5101 ggaatgctct gtcgccaaca caacccgacc ggcaagctcc ctgcgcaatt atgggataaa
     5161 caagcggacg aaaacctgcc gaacattgcg atcaccgatg aacagagccg ttgggcagca
     5221 ggcagacgga ttggcgcaag cagctctttt gcctttggcg gcaataacag cgtactgatt
     5281 ttgggagaaa gcgatgcata aattcacctt ccctatcgaa caaattgcac cattggttcc
     5341 tcacagtggc aatatgatcc tactcgaccg cattaccgca ttcggcgagg attttttgac
     5401 cgctgaaacc aatattcgcc ctgacaatcc gctgatcaaa cacggaaaac ttgccaccta
     5461 cgccagcatt gaaattatgg cacaagccgg cgaaccagtt cgcttaggct acctgctcgg
     5521 cactcgcaaa ctgcacctcc accgccaaga aatcccgatt ggctcacagc tacaaattca
     5581 gatcaaaatg tctattcaag atgccaccgg cttcggcgta ttcgactgcc aactgatcga
     5641 tttagccgac aaccaagtgc ttttagaagg ggcgctgaat gtgtttagtc cgaaatagag
     5701 ttggtagtag taagtagtag taagtagtag taagtagtag taagtagtag ttggtagtag
     5761 ttggtagtag ttggtagtag ttggtagtag ttggtaggaa attcgttatg caagaggcct
     5821 atgacattca acatctttgt actcgcaaag aagataaatc ctttgatcgc aaaagtgcga
     5881 gaaaagaacc gaaagagatt ttgcggcatt tgattggttt tgcgaatgca gacggcggtt
     5941 tattagttat tggtattgaa gataatggcg aaattaccgg ttttaactac caaaaagccc
     6001 atcagccgga agaatttatc cacgctctgc acaatttaca aaaaatgccg ataccggtga
     6061 cctatcagca aattgaggtg gtaaatagtc gaggtttagc ggattttgtc ttattatttg
     6121 aagttacagc ttcgagtggg agggtgatta cctctcacga tgatcgggtt tttctacgct
     6181 caaaagatca aactttggaa ctcaattttg aacaacgttt gcaattagag tacgaaaaag
     6241 ggcagcgtgc ttttgaagag caaccgatag cacgcgctga ttttactgat ctggatatgg
     6301 atttattaga acagtatcga cagaaattac acacagatcg ctcaatagaa gagattctga
     6361 aagcccgaga tttaattaac aaagaagggc aaattacgaa tgcctgtatt ttgctatttg
     6421 ggaataaccc gacacgttat tttcctaatg cccgtttgcg ttttttgcgg tatgaaggta
     6481 aagcacctct gaccggtaga acttttaatg tcgtcaaaga atttatgctt gaagagccta
     6541 ttcctaagtt attggagagt gctaaggcag tattaaaaag ccaattacga gagttccaac
     6601 gtttagatga aaacgggcaa ttcacaaaaa ttgatgagta tccagaattt gcttggttag
     6661 aaggtattgt caatgcgatt gcacaccgta gttacagtaa tcaaggggac tgcattcgca
     6721 tttcgatgtt tgatgaccgt ttggaaattt ttagtccggg tggattacct cgccctgtga
     6781 cgcttgaaaa tatgtactat acccgttttt cacgcaatcc gaagattgca cgagtgctgt
     6841 atgaatttgg ttgggtcaaa gagctgaatg aaggtgttaa tcgtatggcg gaggaaatga
     6901 acgcttacca acttaataca ccttcttact ttgaacccaa tgaaaatgcg gtcagactga
     6961 ttttagaaaa taatattgaa catcggacat taagggcatt agaacgaata gaagaaattt
     7021 ttacgcagga tattatggcg acattaaata aaaatgaagt tactatcctg caatatttat
     7081 atgaaaatga aatgattact gtaaagattg ctagtgagat attagatcgg agtaaagaat
     7141 tatctcgtag aatattaaaa agtttagaac aaaaaaatat tctcacttgg cacggcttaa
     7201 gtccaaaaga tccaacccaa tactacacac tcaagaaata gtaggaaata taagatgaat
     7261 aatacagttc tcatcaccgg ctcaagccgt ggtatcggta aagcgattgc tttaaccctt
     7321 gcacaacaag ggtttgatat tgtggtacat tgccgttctc gtgtggaaga agcggaagcg
     7381 gtggcacaag cggtcagaaa tctcggaaaa aatgccagag tgttacagtt tgatgtggcg
     7441 gatcgtgaaa aagtgcggtc gattttagag gcagatgtgg aacaacatgg tgcctattac
     7501 ggcgtggtgc taaatgcagg gcttacccgt gataacgcct ttcctgcttt aaccgatgag
     7561 gattgggata gcgtgttgcg taccaattta gacggttttt ataatgtcct gcacccgatt
     7621 atgatgccga tgatccgtcg ccgtaaagca ggcaggattg tctgtatcac ctcggtttcc
     7681 ggcttgatcg gcaaccgtgg gcaggtcaat tacagcgctt caaaagccgg cattattggt
     7741 gcggcaaaag cactcgccat cgagcttgcc aaacgtaaaa tcacggtaaa ctgcgtggcg
     7801 cccggcttga ttgataccga tattctggac gaaaacgtcc caatcgaaga aattctcaaa
     7861 atgatccccg caggacgaat gggcgatcct gatgaagtgg cacacgccgt tgcgttttta
     7921 atggatgaaa aagccactta catcacacgc caagtcatcg ctgtgaatgg tgggttgtgt
     7981 taactttagg ataataattc gtaggggcgg atccagtacc cgcccgaagc caaaaagaga
     8041 taaatatgaa acgagtcgtc atcaccggaa ttggtgtggt taccgctttt ggtaaagagt
     8101 gggcggaaat tcacggctat caaccgccga aacattggaa ccgcaagcag ttgcgcagcc
     8161 ttgggcgagt gtcgcaattt gtggtggagg ccagcgagcg ggctttagca caggccggtt
     8221 tgttggacga aaacggcgtg attttcgact ggctaaaaga caggcgaatg ggcgtggcgt
     8281 gtggctcgtc cacaggctct accaatgaca tcaaagatgc cgccgaattg ctgatgaccg
     8341 gcaaatcgga cggtttcaat gccaacacct atgtgcgaat gatgccccac accaccgctg
     8401 ccaatattgg cattttcttc gggctgacag ggcggattat cccgacttcc agcgcttgct
     8461 cctcgggcag ccaaggtatc ggttacgctt acgaagccat taaatacggt atgatcccga
     8521 tgatgcttgc cggcggtgga gaagaatttt gtgcctccaa agtctatgtc ttcgacagcc
     8581 tctatgctgc cagccgccgc aacgacaacc caaaaggcac gccacgcccc tatgacaaag
     8641 accgagacgg cttagtgatc ggcgagggag cgggaatgtt cgtattggaa gagctggaac
     8701 acgcgttagc acgaggggca aacattatcg ccgaagtggt gggctacggt gcaaacagtg
     8761 acggttccca cgtcacccgc ccacaagcca ccacaatgca acgctgtatg gaacttgccc
     8821 ttaaagatgc cggtattcaa ccgaaccaag tcggctatgt aaacggacac ggcacggcca
     8881 ccgagcaggg cgacattgcc gaaactcaag ccaccacagc ggtgttcggc aaaattccgc
     8941 tcagctcgca aaaaagctac ttagggcata cgctcggtgc gtgtggtgca ttggaaagct
     9001 ggttcagcat tgaaatgatg cgagacggct ggttcgcccc aacactgaat ttagaaaatg
     9061 tcgatgaacg ctgtggcgac ttggattacc ttcaaggtga cggacgggaa atccacaccg
     9121 attatgtgat gaacaacaat ttcgccttcg gcggtgtgaa tacctcattg atttttaaac
     9181 gttggaaata accgttttca aataatgaaa aagatattat tagtttatta ctcccaaacc
     9241 gggcaactca cccatttggc agaaaatttt gtacaatcgc ttgaacaagc gggcgttttt
     9301 gtggaaaaac tcgcaatcaa accgcaacag gaatacccgt ttccgtggcg gtttatgcgt
     9361 ttttttaaca ccttccccga aaccgtccac ctcacaccgc cgccgattga gcctctgccc
     9421 tttcagcacg aaatatacga tttggtgatc atcgcctatt ccgtgtggtt tctctccccc
     9481 agccagccga ttaccgcttt tttacagagt gaacaggcaa aaaagtgttg aaggatacgc
     9541 ccgtcatcac gctgatcggc tgtcgcaata tgtggctgat ggcgcaggaa aaagtgaaaa
     9601 aactgcttgc cgagagtgag gcgaagctca ttggcaacgt ggtcaaagtg gatcaatcta
     9661 acgactgggc gagttttatc accacaccgc tttggatgct gaccggcaag aaaaaaaatc
     9721 ggcaagctgc cgagcgctgg cattgccgaa agcgaaatgg aagatgtaaa acgcttcggg
     9781 gcaaaacttt tgcacatttt tagccaaaac caaccgcttg acagcaccct tttccgccaa
     9841 atgcaagcgg tcaaaattga cgaaaaattg atgatgagcg aaaaagtcgg tgcgagaagt
     9901 ttccaccttt ggggcaaatt actgatcaag tgcggtcaaa tttcaccgca cttacgccaa
     9961 gcggtactct atttttacat cctcttttta gtgacgttaa ttttaaccgt cgtcccgatt
    10021 tcaacggtga ttaaacggct attacgccca ctgctgaaag aaaaattaga aaaacaaaaa
    10081 cggtattttg ccgagccttc gggcgagtaa acgtacccta cacggaaact ttatgctatc
    10141 taacgtctat atcaccaaca cctccgcctt tttacccaac gcccctgtgt ccaacgatgc
    10201 aatggaagcg gtgttgggaa tggtggggga aacgccttcc cgagtgcgga agatgatttt
    10261 gcgttctaac ggtattcaac atcgttatta tgccattgac ccgataaccc gccaagcgac
    10321 tcacagcagt accgagttgg cggtgcaagc ggtcaaaaaa ttgggaaatt ctgcaacatc
    10381 agcgacttgc ttggcggtgg ggacatctta ccccgaccaa attatgccgg ggcagggggt
    10441 gatggtacac ggcttgcttg atgccgaacc gatggaagtg ctgaccactg ccggtgtgtg
    10501 cgtggcggga atggcagcga tgaagcacgc ctataatgcc gtacgcatcg gtgagcatcg
    10561 tgctgcgatt gcaacggctt ccgaaaccgc ctccgccatt atgcgcagtg aacatttccg
    10621 tgccgaaatc gatcataaaa aactggacga tgctcgccct gagatcggtt ttgagaaaga
    10681 tttcctgcgt tggatgctct cagacggtgc aggggcggta ttgcttgcca atcagccgaa
    10741 cgaaaaaggg ttgagcctca aaattcactg gatcgatctg gtctcttacg ctaacgaaat
    10801 gccggtttgt atgtacgccg gtgcggaatt tattgacggc aactacaccg gctggaaagc
    10861 aatgagcgaa gcccaacgtg ccgaatgctc ggcaatggcg gtgaaacaag atgtcaaact
    10921 gctcaacgaa aacatcgtca aatacacggt ggaaaaagca ttggagcgga ttatcccgaa
    10981 atacaagctc aacgctgaag agattgacta cttcctgccg cactactcgt ccggttcctt
    11041 ccgagataaa ttattggaag gtttacagcg gatcaatttc ctgatcccac aggaaaaatg
    11101 gttcaccaac ctgactacca aaggcaacac tgggtcagcc tcgatctaca ttatgctcga
    11161 tgaatttatc cgcacccacg atctcaaaca gggacaaaaa atcctctgct atgtgccgga
    11221 aagcggacgt ttttcgtctt gttttatgct tttggaagcg gtggtaaatt cgtaaaatcg
    11281 tagggacggg ggctattccc gtccaacaat catcggaaaa cgtatggaca tcaatgacat
    11341 tccccaagac aacagcaaaa tcttcaacgg cgaacgcaaa gtgatctacg cccgcaatcc
    11401 gcaaccagaa atccgccaaa tgatctcctc tattctcgct tgctacgctt gggacgaaaa
    11461 caacccgttt gtagcgaagt ctgatgcacg gttgaaggca ttggcggaag ttgttggtaa
    11521 agcagaaatt ggcgattaag tttgattttg tgctttacag ggtcgcagat tatctgtata
    11581 atttgcgacc ttaacttgcg agagcaagct gatggtgctt agcctatcta agccccgtct
    11641 aagactgtag gggtgaaaac ttaataatcc tacatagatg gtgaacagac agaattctct
    11701 gcttctggac accttaggct caaagaagat tgcagtttgt agcaatgcaa gcggtaatat
    11761 ttttgggatt ttttgtaaat tctgcttgaa aaggcagagt gtatcaggag ctaaaaccaa
    11821 tggcattaaa tcttcaagac aaacaagcga ttgttgctga agtaaacgaa gctgccaaag
    11881 gtgcactttc agctgttgtt gcggattctc gcggtgtaac agttgaaaaa atgactgagt
    11941 tacgtaaagc agctcgtgaa gctggtgtga caatgcaagt tgtacgtaat actttattac
    12001 gtcgtgcagt ggaaggcacc gagttcgagt gcttaacaga tacgtttacc ggtccaactc
    12061 ttatcgcatt ctcaaatgaa cacccaggtg cagcagcacg tttattcact gaatttgcga
    12121 aaacaaacaa agagtttgaa cttaaaggtg cagcctttga aggtaaaaaa caagatgttg
    12181 cattcttagc aaccttacca acttacgaag aagcaattgc acgtttaatg ggcacaatga
    12241 aagaagctgc ggcaggcaaa cttgttcgca ctcttgcagc attacgcgat caaatggaag
    12301 cagcagctta attttaagca ctcttcttta ttcgtttaac aacttatttt actttaggaa
    12361 tcgattgtta tgtcattaac taacgaacaa atcattgaag cgattgcttc taaatcagta
    12421 tcagaaatcg ttgaattaat cactgcgatg gaagaaaaat tcggtgtttc agcagcggca
    12481 gcagtagcag cagctccagc agcaggcgca gcggcagcag aagagaaaac tgagtttgat
    12541 gtagtattag cagaagctgg tgcaaacaaa gttgctgtaa tcaaagcagt acgtggtgca
    12601 acaggtttag gcttaaaaga agctaaagac ttagtagaat cagctccagc tgcattaaaa
    12661 gaaggcatct ctaaagcaga agctgaagca cttaagaaag aattagaaga agctggcgcg
    12721 aaagtagaaa tcaaataatt tttgatttat tttgtcagaa cttcaagttc aacaaacccc
    12781 gatggaaaca tcggggtttt gttttttcag aattgtgtaa tgtagatatg attaccttta
    12841 tccattactt gcgcagcagt gaaaaatgta aaaattccac aaagtgatct gcccagtgca
    12901 tttcgtggtg gatttcattt gccataatcc gcaggcggat attgtctaac ggcacgccaa
    12961 tccgaatcag tgcttggtag taattgagcg aacaatcaat ataggtttgg ttcgtattag
    13021 agataaagtg cgaatcgatt tcatcgcctt cgttagtgcc aacctgaata aacactttgg
    13081 tattgggttg taacggatat tggtgcgtaa aacgcagaaa atccggctca ctaaaccaag
    13141 aggcaagcga aaacacccct aaatgcccga aaatatgtgg gtagcggctg cccatataag
    13201 cggtgataat gccacccatt gaactgcctg ccaataaggt atgttcttgc tgaggcttgg
    13261 tgcgatagtg aatatcaata aagggtttaa ccgtttccac cacccatttg gcatattcct
    13321 cccctaagcc tccggcattt cgggtttcag gggttgagcc ggtggttgtt ttccacgggc
    13381 cgtattcatt taagcgatta gctcctgcgt tatcaatgcc gacaataatc atcttcggta
    13441 attcttggtg tgatttaatg gttggaataa ttttccaaga atgccccgaa taagactctt
    13501 tgctgtaaaa cacattttgc ccgtcgtgca tatataacac cggataacgt tcccaacctt
    13561 cgttgtgata atccttcggc aatagtaccc gaatgcggcg gtgatggtgt aaatagggaa
    13621 catataaaaa atgctcctgc atttctaaat agtggttaag tggtttttgt accatcagtc
    13681 tgtccttttg cttaaattgt ggcgtatttt acaccttaat cagtctgagt taaaacaagc
    13741 ggtcagaaaa agtcaaaaat ttgcaaattg ctgttaccta acgcaatcgg ttgccttatg
    13801 cttttgagga atataaaaga gaaactgtga tctaagtcac agcaattttt attcggtttg
    13861 actaaaatcc gaactatcac ttaatagaga ggaaaatgtt atgcaggctt cattacaaga
    13921 tattcttgag gtagtaaaag caaaaaatct cacttatcag caaaaattga tgagtttagg
    13981 caatattgct gagcggttat ttaacccgat tgaactactt ggctacactg aagaagagtg
    14041 ggaacacatt gaaaatcaga tgatttgcga tttaaatgaa ggttatgcga tttatcgccc
    14101 acgttatatt ttgcctgatt ataatgtgta tatgcaaaaa ggttgccaat tcttagattt
    14161 acctccgcca accaatttag atgaagcctt agacggcttg ttaattctgt attcacacgt
    14221 tccgtccatt actacttttc cggtgtatat cgggcgttta gaccaactgt tagaaccgtt
    14281 tataaccgat gaagagcaag attacattaa aatcaagcgt ttcttaaatc atatcgataa
    14341 aaccatttcc gattctttct gccacgctaa catcgggccg aatgacacca aagccggtcg
    14401 tttaatctta aaagcggtga ttgagcttga aaacattaca ccgaatatga cgattcgtta
    14461 tgataaaacc aaaacatcgc gtgaatttgc cgaactggcg gcgaaagcgt gcttgttggt
    14521 gtccaaaccg tcttttgcta atgatgccta ctacattcaa gatttaggcg aagaatacgg
    14581 aattgcaagc tgttataacg ccttaccgga atgtggcggt gcttacactc tgacccgcct
    14641 gcgtttaggt actatcgggc gtgcttgtgc gagtgttgaa caaatggtca atgagatgct
    14701 gccgaaagtg gcaaaactcg ccctttccac gatggataaa cggcataaat tcttggtgga
    14761 agaaagcaat ttctttgaaa gtagcttctt ggttcaggag ggctttatca aacgtactaa
    14821 cttcacaagt atgattgcga ttgtaggtct tgctgatgcg gtgaatcatt tactcaagca
    14881 agaaggttta aatgaaacct ttgggcaaag caaacgtggc gatgaaattg cgacgctgat
    14941 tatggataag ctccaagcag taaatgacgc tcataaaggg ctgtatgttg aacgaaccaa
    15001 taaccaatat ctgttacacg ctcaagtggg ggcaaataac cacgctgaag acaaaatgaa
    15061 cacgccggca caccgcattc gtgtgggcga agagccaacc ttattggcac acttaaaaca
    15121 gtctgctcct ttccataaat atttcccatc cggtacgggg gatttattcg cctttgacca
    15181 aacctacact gatcatttag atgcggtggt ggatattatt gacggctcgt tcgcccaagg
    15241 ttatcgctac attaccactt acctgaaaaa tactgatttg attcgagtga caggctactt
    15301 ggtgaaaaaa agcgaggttg aacgttatcg caaaggcgaa gcggtattgc gtgataccac
    15361 ttggtatggt tcaggcaccg atgattgtgc gaaagtgttc gaccgccagc tgcgtgatga
    15421 agaaaatgtg aatgcagggt aacaatggct gcactttctg agatttttgt gccgttacac
    15481 cgcattatcc ccttctcgaa tgtagaaggg cagggcaatc gtaccagcat ttttctacaa
    15541 ggttgcaagc tgaattgcct gtattgccat aacccggaaa ccattccacg ttatagcaag
    15601 gaagcaaaac aggttagctt gcaatacttg tacgatcaag tgatggaggc agttcccttt
    15661 attcgaggag tcactatttc gggtggagag ccaaccattc atcataaaaa attagtgcca
    15721 ctgttcgagg cgttgcgtga gcaagggcta acctgttatt tagacagcag cggctttttt
    15781 gattttgagg caacagagcc gttaattaat gttaccgata aatttctgtt cgatctcaaa
    15841 ggagatgggg cgggcttaca aacgctatgc tttgatcgga aaaaccaagc cggcaaagtg
    15901 cctgaacagg ctcttccaaa cgtgaatcat attaaacctg aaaacttggc tcgcaatttg
    15961 caaaatttag ccaaattatt accgcttgat aaggtggaag aagtacgttt ggtttacctc
    16021 aatgagtttt ataacgccca ccatttgctc gaaaaagtgg ctgacctact taaaccttac
    16081 ccagatgtac tacttaaaat tattcgggta cacaccaaag gagcgagaga tgaaacaggc
    16141 ttggctcgtt ttgtacccac tatcgagcaa accgatgaac tggcagcatt tgcaaaacgg
    16201 cacggtattc aaaaagttat tactatttac taatggagac ttacgatgga tatactaatt
    16261 agcataattg gtgtttttgt gttattaggc ttaggtgttt tattatcaaa taatcgcaaa
    16321 gccattaaat ttcgcacaat tttgggcgct ttggcgattc aaattggttt tgccgctctt
    16381 attctttact ttccagcggg aagaaatgca ttgctagcta ctgcaaattg tgtgagcaat
    16441 attattaatt atggtaacga aggtattagc tttgtgtttg gcaatttagc gaatccgagt
    16501 aatagcagca ttggttttgt gtttgcggtt aaagtattgc ctattattat cttcttctct
    16561 gcgttaattt caatgcttta ctaccttggt gtgatgcaat gggtgattaa aattattggt
    16621 ggagcattac aaaaaacact aggcacttcc aaggcagaat caatgtcagc ggcagctaat
    16681 tatttttgtt ggtcagacag aagctcctct agttgtaaaa ccatacatta gccgtatgac
    16741 agagtcagaa ttatttgcca ttatggttgg tggaactgct tcaattgctg gttccgttat
    16801 ggctggttat gccggaatgg gcgttccttt aacttatctt attgcggcct cctttatggc
    16861 tgcaccagct gggttactat ttgctaaatt aatgtatcct caaacggaaa ccttggagag
    16921 taaagcagat gacaatgttg aagttgaaaa gccaaataac gtggttgagg ccctagctaa
    16981 tggtgctagt gcaggtgtta ctttggctat taatgtcggt gctatgttaa tagcatttat
    17041 tgcagtgatc gctttattaa atggctttat tggtggcatt ggtaattggt ttggttatga
    17101 ggggctaacc ttacaaattt tattaggtta tttattccaa ccacttgctt tcttaatcgg
    17161 tgtgccgtgg caagatgccg atattgcagg tcaaatgata ggtatgaaac ttgctgttaa
    17221 tgaatttgtc ggatatcttg agtttgctaa atatctacag cctgatgctg tggtgcaatt
    17281 aagcgataaa actaaggcaa taattacttt tgctttatgt ggttttgcaa actttagagc
    17341 aattgcggta ttgattagtg gtatcggaag tatggcacca agtcggcgtt cagatattgc
    17401 tcgtttaggt ttaaaagcag tgattggcgg cacattggcg aacttaatga gtgcaactat
    17461 agcgggtttc tttattggtt taagtggtgc cacattaggt tgatacaagc ggttaaattg
    17521 actcactttt ttgcaaaaag cccaagtaag ttaaatgact acttgggctt tgtttaatga
    17581 actagctctt agtttcggtt tttgctgtgt tatccttttc cgctttggct gtctgtgttt
    17641 tcgaagttgt ctgttttttt gcggattggc tagcagtagc tgcttttctt actttctttg
    17701 ttttaacttc tacttccgca tttttagagt ttttatccgc taaccatgcc tgcactttag
    17761 ctaagaatat ttgtcccatc gggcgaggat cgccagaaaa taatgtttgt aatttattat
    17821 tttcaataat gtcgcgtcta attgctaagc gttcttcgtg gttttccgct aaacgaatag
    17881 cttgagtaac gtattcctct gctgtttggg taattaacca attagatagc ccgaggcgct
    17941 taaataaacc ttcatcaata tgttcatgaa cttcagggcc tgttttacag acaccaacta
    18001 aaccaagcgt aaccatatcg ataatgccgt ttgtatttcc aaatgggaat ggatttagca
    18061 tcatatcgca attatgtaga atatttagat attcatcata tggcgaatgt gggtgtgcgg
    18121 ttgcatcatt tcctaaatag cttttgataa aacgttccac ataaggatgc gtaattccac
    18181 tagattgccc taatgcaaaa tggaaatgag tttttacctt cgccctatca cgaatggctt
    18241 ttaatgcctc taagaaatat gggtttaatt tcatagtggt tgatgcaatt ccgatatgga
    18301 taacctctgg attttcacgt aggttataga ccacattttg aggcgctaat gcggaaggca
    18361 catagggtag cgcatctttt ggaagacgta ataattgttc actaaagcag ctttcggaac
    18421 caacatagtc atcttctacg atgacatatt caataaaatc agagtgtgta gttgccggat
    18481 gacctagcgc aacagcttga attggtgcta ggcgagtgtt actagcgaaa atagtggtta
    18541 aatccattcc aatgctaggc atatagaata cggctggatt atttttatca cagatatctt
    18601 ttaaaaagct taagcggtca aatagtgagc catcttgtgg taataaatga aattcgtcga
    18661 atacctgttg ccccatttca tctacagcat cactgcctag cccaataaga tggaaatgtt
    18721 cgcgtgcagc aaccattgag gttgaatgag tacgataaat agaatgtgat gaatggaaat
    18781 gctccaataa tactaccatg acaggtttat tatttctggt accaagctgt tcaatttttc
    18841 tatcttccca accgacagaa agtaaatgac gacgaattac agcatttaac gaccgtttta
    18901 cattatgttt atttgcttca atatcataac tgcaatgcat ataaacatcg tgtgaaatgg
    18961 cgctcggtag cttgttcaag ttttcaattt gcgctaattt ttctgggaac cattgcaaaa
    19021 tagccgcgcg ttttgcaaaa gcagaggatg taccaataaa tctaggtgat tggagtgcaa
    19081 aacaaagtga tgcggtgaga tctgatgaag cattccatgc agcatctaga ttcaataaaa
    19141 tattagattc cggtaagtat aaaatacaga acttaattag tgcttgtaat gtggcatcta
    19201 aatcaaccgt atttggatta gcagattctt tatttctatt gtaagtacgc aagatatggt
    19261 ctgcgtttac gtatggagaa ctggcaaaaa ttaatgctat ccagcgttgt aatgtcagaa
    19321 aacgcatagc accgtgttcg gagatctcta atgcaggatc ttcaaacaag cgagtaatcg
    19381 ctaccgccat tcgagtacag aaatagatag ctttatcatt ttctaaattt tcaatctgct
    19441 ctggcatatt caattcaata ttggaaatgc caccaaaatt actatctaat tttgaaagga
    19501 tagacagtag ctcggtacaa gccgattcat aatctttttt cgcgaccgcc tgctcaaaac
    19561 ggatgacgct aggcatattt tctgctgaca taatgtatcc ttagttgttt aaaattcccc
    19621 acagatcata ctcatctgca tgtgtaatgg ttactgaaat aatttgccct actgtcacta
    19681 attggtttga aggattatct acatagacta caccgtcaat ttccggtgca tatgccattg
    19741 agcgaccgat aatgccttct tcgtcaatgt catcaataat tacttgtacg gttttgccga
    19801 tttttgcttg taagcgagca gcggaaattt cttgttgtag ttgcataaaa cggtggaaac
    19861 gttcctcttt tacttcttcc ggaacttgat ccggcatatc ggtagcaaca gcaccttcca
    19921 ctgggctgaa tttaaagcaa ccaacacgat ctaattgtgc ttcttttaag aaatcgagta
    19981 atagttggaa atcttcttct gtttcccccg ggaagccgac aataaaggta gaacgcagag
    20041 ttaattcagg gcaaatttca cgccattttt taatgcgttc taaggttctt tcgattgaac
    20101 ccgggcgttt catcgctttt aagattttcg ggctggcgtg ttggagtgga atatccaaat
    20161 acggcaaaat tttgccggca gccataagcg gaattaaatc atctacgtgt gggtatgggt
    20221 aaacatagtg caaacgcacc cacacgccca ttgagcctaa ttgctcacat agcgtaatta
    20281 aattactttt aatcggcata ccgttccaga aaacggtttt ggttgcttct tcttttttgc
    20341 gatctaacga ataggctgag gtgtcttgtg agacgattaa cagctctttt accccggcat
    20401 caactaggcg ttttgcttca tctaatactt gcacgataga acggcttgct aaatcgccac
    20461 gcattgaagg aataatgcag aaagtgcagc gatgatcgca gccttcggaa attttcaaat
    20521 aagcgtaatg cttcggtgtt aatttaatcc cttgttttgg cactaagctg gtgtaagggt
    20581 taaattccgg ttttggcaca tatttatgga cgtggttcat taccgcctca tagctatgtg
    20641 gccctgtaat ttccaatact ttagggtgta cctcacgaat ttggttttct tttgccccta
    20701 aacagccggt gacaattact tttccgttag cttctaacgc ttcaccaatg ctttcaagag
    20761 attcttgtac tgcggaatca ataaaaccgc aggtgtttac aatcacaaga tccgcatttt
    20821 cataactagg gatgatgtta taaccatccg cacgcagttc ggtcaaaata cgttcggaat
    20881 caactaaatt tttagggcag cctaagctga taaaaccaat atttggtgaa gcactcatat
    20941 aactctcaat aactaaaaag aaaaatgcct acaatttttg tctgtaggca aaaaactaac
    21001 aagattctac aaaatcttgc ccaaaatagc gataacaagc ggtcaatttt taataaacat
    21061 ttacaaactt gaaatttcgc taacaattaa aattaatcga tattatttag cattgctaat
    21121 acaatcgtgg aagatttttc tgcggctaat ggcagaaatt cttcaaaaga aaggtgggat
    21181 tctttgtccg ctacatccga tactgctctt acgattacaa atggaaggtt aaaaccgtga
    21241 caaacctgcg caatggctgc tgcttccatt tcgactgcaa cgacattcgg gaaattctga
    21301 cgaatttgat taatcttatc attaccgtta ataaaggcat cgccgctaca gattaagcct
    21361 gaaaccacat tgtaaccggc ttttttcgct tccttttgag ctaattcgac taattgctcg
    21421 tttggcgaaa atgttggcgg gtttgccggt agttgcccga tttcataacc aaaggcggtc
    21481 acatcaacat cgtgataacg aacttcggtt gaaatcaaaa tatcacctac gtttaaatct
    21541 ggatctaatc caccggcaga gcctgtatta ataatcatat ccggctgggt gagctgtatc
    21601 agtaaggtag tgccgatggc ggcagcaact ttgccgatac ccgattgtaa taatgccaca
    21661 cgggtattgt taattttgcc ttcaaaaatt ttacaacctg ctaattcagt gactttaggt
    21721 tcaaccatta cttggcgtaa aatctcaatt tcttgtgcca tcgcaccaat aataccaatt
    21781 ttcattactt tccttctcta atgtattgtt tattttttga aaaaaacgct attttaacag
    21841 cattaatagt gaggaggggg tgtttcttct gcttgtgaag caacttggct tagttgagcc
    21901 cctttcaatt tctcagcaat atggcgaatc tgagtttgta acttatcaat tgcaaattgt
    21961 tgctcaataa gtgcttgatt taactcttca ataatcagct cttggaaagc aactttagtc
    22021 tccaattcgg taatttttgc cgataatgta taattattat ccatttttta ctaaaaaaag
    22081 gttgtgtgaa aaggaatta
//
LOCUS       DANLZC010000026        20361 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.026, whole genome shotgun sequence.
ACCESSION   DANLZC010000026 DANLZC010000000
VERSION     DANLZC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20361
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.026"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..252
                     /locus_tag="PXE44_000779"
     CDS             <1..252
                     /locus_tag="PXE44_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588311.1"
                     /translation="SYSGETTASPLLKLDTERNINLYFQAIHKCTPDKIHTKIINIPS
                     EGNAKELWTATGCNKTQSFIITYRNDGVGGTFINMTTTP"
     gene            269..1840
                     /gene="guaA"
                     /locus_tag="PXE44_000780"
     CDS             269..1840
                     /gene="guaA"
                     /locus_tag="PXE44_000780"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL5588312.1"
                     /translation="MTNIHHHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQ
                     IREFNPTGIILSGGPESTTEENSPRAPEYVFNAGLPVLGICYGMQTMAMQLGGLTETS
                     DHREFGYASVALKSADQLFANLNDDLTACEPKLDVWMSHGDKVTKLPPHFTITGVTPT
                     CPIAAMSDESRRFYGVQFHPEVTHTKQGLALLKNFVVGICGCETNWTAENIIEDAVKR
                     IREQVGDDEVILGLSGGVDSSVTALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFG
                     NKFGLNIVRVNAEDRFLEALKGLDEPEAKRKTIGKVFVDVFDDESKKLSSVKWLAQGT
                     IYPDVIESAASKTGKAHVIKSHHNVGGLPDYMKLGLVEPLRELFKDEVRKIGLALGLP
                     AEMLNRHPFPGPGLGVRVLGEIKKEYCDLLRKADAIFIEELHKAGWYYKVSQAFTVFL
                     PVKSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDLLGKISNRIINEVNGISRV
                     VYDVSGKPPATIEWE"
     gene            complement(2001..2396)
                     /locus_tag="PXE44_000781"
     CDS             complement(2001..2396)
                     /locus_tag="PXE44_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcB family protein"
                     /protein_id="HDL5588313.1"
                     /translation="MEQWTTDVWAAIMAAFIVGAVIGCTLVRVFNSSVRKQHELEKEL
                     KQTKTQVDEQKQKLEQHFEQSAQLLSSLASDYKKLYTHLAQSSEKLLPEASKNITFFQ
                     QTKLPIPAEKTEEDQPRDYSEGSSGLLKS"
     gene            2695..3123
                     /gene="rplM"
                     /locus_tag="PXE44_000782"
     CDS             2695..3123
                     /gene="rplM"
                     /locus_tag="PXE44_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL5588314.1"
                     /translation="MKTFVAKPETVQRDWYVVDATGKTLGRLATELARRLRGKHKPEY
                     TPHVDTGDYIIVINADKVAVTGKKQTDKIYYWHTGYVGGIKDATFKEMIARRPEAVIE
                     IAVKGMLPKGPLGREMFRKLKVYAGSEHNHQAQQPQVLDI"
     gene            3140..3535
                     /gene="rpsI"
                     /locus_tag="PXE44_000783"
     CDS             3140..3535
                     /gene="rpsI"
                     /locus_tag="PXE44_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL5588315.1"
                     /translation="MTAANQNYGTGRRKSSSARVFIKPGSGNITINQRSLEVYFGRET
                     ARMIVRQPLELVELTDKLDLYITVKGGGISGQAGAIRHGITRALMEYDETLRPALRAA
                     GFVTRDARRVERKKVGLHKARRRPQYSKR"
     gene            5217..6937
                     /gene="ltrA"
                     /locus_tag="PXE44_000784"
                     /pseudo
     CDS             5217..6937
                     /gene="ltrA"
                     /locus_tag="PXE44_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341372.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            7126..7902
                     /gene="yaaA"
                     /locus_tag="PXE44_000785"
     CDS             7126..7902
                     /gene="yaaA"
                     /locus_tag="PXE44_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDL5588316.1"
                     /translation="MLAIISPAKTLDFKTNIDGFAFSQPNLTACSQELIDICKQLSPA
                     ELGSLMSISDKLAALNVARFAEWQLEHNEQNAKAALFAFKGDVYTGLEAETLSKAQVE
                     YAQQHLRMLSGLYGLLKPLDLMQPYRLEMGTKLANPKGKDLYAFWGNIITENLQQAID
                     EQGDDILVNLASDEYYGAVKPQNLNATIIKPVFLDEKNGKYKVISFYAKKARGMMVRF
                     ILDTQPTSIEQLKQFNYGGYWFDADSSSESELVFKREEQA"
     gene            7902..8465
                     /locus_tag="PXE44_000786"
     CDS             7902..8465
                     /locus_tag="PXE44_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HDL5588317.1"
                     /translation="MSAIKKGITLVALSLAGCVSQSPQFAVPDAIHFGQDTFVKVTHS
                     QIDEMQHLLYLPEKSEKNPENWQKGILFFLDKNSKNQTLEQRAAFRQASFAKKPELEA
                     KVEIQQNELHSSVIYPPIERFNNVMLEVTRGRNLACGYGQIQFADKREVGKTVAKKSP
                     NLTAYSQEIARLSLELNQLGWQVQCSK"
     gene            8474..9379
                     /locus_tag="PXE44_000787"
     CDS             8474..9379
                     /locus_tag="PXE44_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL5588318.1"
                     /translation="MNEKYSKLVKNAANLAIIVAILLIVIKAFAWWKTGSISILAAMT
                     DSVVDLFASLTNILVLRFALQPADENHAFGHGKAESLAALAQSAFISGSAVFLLLQGF
                     HKLTNPELIAQSSIGVIVSIVSIVITALLVFYQRYVVTLTKSPAIEADSLHYQTDLLM
                     NAAILVAMLLNSAGFVYADAVFAIGIALYIAFNAVKMFWCAVQTLLDQALPDEEIERI
                     KEIASQHPNILGIHDILTRRAGAVRFIQLHLELDDHLTLLVAHEITDSLEQKLLAAFP
                     MSHVIIHQEPTTVVKQEFQDMAENI"
     gene            9379..10131
                     /locus_tag="PXE44_000788"
     CDS             9379..10131
                     /locus_tag="PXE44_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon-containing protein"
                     /protein_id="HDL5588319.1"
                     /translation="MYQAIVHFSYHNFEQDPVTAISQILNQWLYNGQVIGREMPITFH
                     QNEFQARVCVPTQESLLPQYNSDEVNEALQQAVEIGVIFASFELVGRDYQGEESSHNR
                     SPAFQILYTTHLDTCSPLYDGEQFAPIPLYSLQDQSLSEAILEWQQNWQACDLLQMNG
                     NLLECHALVQISDETSDLAMIGRDLCQQIEQKTHIPTFYYLYRLGRDESEYQRKCPCC
                     GGEWKLTEPLHDIFHFRCEPCRLISNLSWELL"
     gene            10232..11557
                     /locus_tag="PXE44_000789"
     CDS             10232..11557
                     /locus_tag="PXE44_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL5588320.1"
                     /translation="MLIFNLIVFAAILFLLAKIYKSTQKLGQTVFIGLFLGLASGAFL
                     QTFYEKATVDATLDWINLIGNGYVRLLQMIVMPLVFVSILSAITRLKEAGSLGKISFS
                     VLSVLLVTTAIAAAIGIALVYLFDLSAEGLVAGSRELAAQEKVVGRVEQVSNLSVPSM
                     LLSFIPKNPFLELTGANPTSIINTVIFSALLGIAALSLGKEDKALGERIAQGVETLNK
                     LVMRLVRFVIRLTPYGVFALMIKMAATSQWSDIVNLGSFIVASYIAIGLMFIVHGILL
                     FVAKINPLDYYKKVLPTLSFAFTSRSSAATIPLNIETQTNKLGNNSVIANFSATFGAT
                     IGQNGCAGIYPAMLAVMVAPTVGMDPFSLNYILTLILVVAISSFGIAGVGGGATFAAI
                     VVLSTLNLPIELVGLLISVEPIIDMARTALNVNGAMVAGTLTNKWVKAE"
     gene            complement(11617..12276)
                     /locus_tag="PXE44_000790"
     CDS             complement(11617..12276)
                     /locus_tag="PXE44_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5588321.1"
                     /translation="MFNISKQQVLDAFHFRSACRSYDPNKKINKEDMDYILELGRLSP
                     SSVGSEPWKFLVLQNPETRQKIAPVSWGIKHPMEEMSHLVVILTKKNVRYDSVYMRDM
                     LAKRGLTYEQIEASLVRYKSFQEEDMKLLSERELFDWSTKQTYIALGNMMTGAALIGI
                     DSCPIEGFHYDTVNQILSDEGLFDLNEYGVSCMITFGYRNKEIKKKSRKPTEEVIAWI
                     E"
     gene            12505..13491
                     /gene="pheS"
                     /locus_tag="PXE44_000791"
     CDS             12505..13491
                     /gene="pheS"
                     /locus_tag="PXE44_000791"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL5588322.1"
                     /translation="MQHLKDLTNKAREALDKIDQNLEALEEFRVEYFGKKGHFTTLMQ
                     ELRNIAAEERPAIGAKINEAKQVVLDVLNAKKEEWEQAVLNAKLANESIDVSLPGRKT
                     ELGGLHPVSITIERVVKFFSELGFTVANGPEIETDYYNFDALNIPDHHPARADHDTFW
                     FDANRLLRTQTSGVQIRTMENVKPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKK
                     ANFTELKGLIHDFLKAFFEEDLQVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMV
                     HPNVLRNVGIDPEEYSGFAVGMGVERLTMLRYNVTDLRSFFENDLRFLKQFK"
     gene            13539..15926
                     /gene="pheT"
                     /locus_tag="PXE44_000792"
     CDS             13539..15926
                     /gene="pheT"
                     /locus_tag="PXE44_000792"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL5588323.1"
                     /translation="MKFNESWLREWVNPAISTEQLCDQITMLGLEVDDVEPVAGAFSG
                     VVVGEVVECAQHPDADKLRVTKVNVGGERLLDIVCGAPNCRQGLKVACAVEGAVLPGD
                     FKIKKTKLRGQPSEGMLCSYSELGIKEDHSGIIELPADAPIGQDFREYLNLNDVAIEI
                     SLTPNRADCLSIAGIAREVGVINRVDVKAPTITDVKATISDKVSVELQAPEACPRYLA
                     RVVKNVNVKATSPLWLQEKLRRCGIRSIDPIVDITNLSLLELGQPMHAFDAAKIEGGI
                     QVRMAKEGEELVLLDGTNAKLQSNTLVIADSKGALAMAGIFGGEASGVSENTKDVVLE
                     SAFFAPLAITGRARQYGLHTDASHRFERGVDPELTRQAMERATALLLEICGGEAGEIV
                     EAVSQENLPKRNTVTLRRSKLDSVIGHHIDDATVTDILTRLGLNVTFADNSWTAIAPS
                     WRFDIEIEEDLIEEIARIYGYNSIPNNSPLAHLTMKGTPEKLLEANRIRMALVDSDYQ
                     EVITYSFVDPKKQSLLHPQQEALILPNPISSEMSAMRVSLLTGLLDTIVYNQSRQQNR
                     VRIFEAGLRFIPDSNAESGVRQEFVFGAAIVGDKRPVHWENKSEAADFFDLKGDIERI
                     LSLTSIRHDLQFVAKQFPALHPGQSAAIMLDGKEIGFIGTVHPSIVQKFGIKGKPIVC
                     EILASAIAERPVTQAGEISKFPANNRDIAVVVDESVPAGEVLEACRKAGGNKLVAVNL
                     FDVYRGSNLESGKKSLAISLTVQDNEKTLEENEINAVIQAVLAELATRFNAVLRD"
     gene            15984..16280
                     /locus_tag="PXE44_000793"
     CDS             15984..16280
                     /locus_tag="PXE44_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDL5588324.1"
                     /translation="MALTKIEIAENLIEKFGLEKRVAKQFVELFFEEIRSSLENGEEV
                     KLSGFGNFSLREKKARPGRNPKTGENVAVSARRVVVFKAGQKLRERVEQASSQS"
     gene            16319..16840
                     /locus_tag="PXE44_000794"
     CDS             16319..16840
                     /locus_tag="PXE44_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5588325.1"
                     /translation="MKLTKRWLSLGCLLAITAMLVACSSSDTTSKSQVKSKVYRSIYQ
                     SDLSDPIMAISRLSEHQHEWKGTRYRLGGNSKAGIDCSGFMQVTFKDLFGIDLPRTTT
                     EQAKAGERISKDELRTGDLVFFKTGRGPNGKHVGVYVKNGQFLHASTKGGVIYSDLDS
                     PYWSKAFWQARRL"
     gene            16856..17899
                     /gene="degS"
                     /locus_tag="PXE44_000795"
     CDS             16856..17899
                     /gene="degS"
                     /locus_tag="PXE44_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDL5588326.1"
                     /translation="MLKKIGQAVIFGLFCAILILFVAPIIQSKGSSFSLSAFTQKEIV
                     SYNAAVKIASPAVVNVYSRNFSSENETFEVKNLGSGVIMSSDGYILTNRHVIEDADQI
                     VIALQNGAISTNVKLVGDDSLTDLAVLKIEGENLPTIPQNPERKLQIGDVVLAIGNPL
                     NLGQSITQGIVSAIGRKTLTESGRQNFIQTDVSINQGNSGGALINTAGELIGINTLRF
                     GKNTNELSEGLNFAIPINLANQIMSKIIKDGRVIRGYFGVSTDLLDTAKMQGYEKGVI
                     IVNVDKNGPAAKAGIQAGDLVLKVGDIEAFSPSQMMEELAEMKPYTTVKVLVQRDEQL
                     LNFDVTITELQTQ"
     gene            17908..18582
                     /gene="cmk"
                     /locus_tag="PXE44_000796"
     CDS             17908..18582
                     /gene="cmk"
                     /locus_tag="PXE44_000796"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL5588327.1"
                     /translation="MKKIVITVDGPSGAGKGTLCHALAEKLGFDFLDSGALYRITALS
                     AIKKEVPLDDEEKLAEVGRNLDIQFLPENGEIQVILEGENVGDLIRTAEAGQNASKVA
                     AFPLVREALLERQRAFSSEKGLIADGRDMGTVVFPDAQIKLFLDASAEERTKRRVKQL
                     QEKGFNANFDEILAEIKERDFRDRNRSVAPLVPAKDALLLDSTHLSIEDVINQALNHI
                     AQYIKF"
     gene            18674..20341
                     /gene="rpsA"
                     /locus_tag="PXE44_000797"
     CDS             18674..20341
                     /gene="rpsA"
                     /locus_tag="PXE44_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL5588328.1"
                     /translation="MSELSFAQLLEESFAATPRLGDVVKGTVVAIQKGLVIVDTGFKS
                     ESAIDASEFTNAQGELEVQVGDEVDVVLKAVEDGFGATVVSRGDAKRNEAWIALEKAF
                     EEQATVIGFVNGKVKGGFTVELNGVRAFLPGSLVDTRPVRDSLNLEGKEYEFKVIKLD
                     QKRNNVVVSRRAVIETESNQDRDAVLANLVEGSEVKGTVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEVVNVGDEVTVKVLKFDKDKTRVSLGLKQLGQDPWAVIAENHPV
                     NSKLTGKVTNLTDYGCFVEILDGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLE
                     VDEERRRISLGLKQCKANPWEQFAATHSKNDKVSGKIKSITDFGIFIGLEGGIDGLVH
                     LSDISWNVSGEEAVRNYKKGDEVEAVVLQVDAVKERISLGIKQLESDPFTNFVDGTKK
                     GAILTGKVVEVDAKGVKVELEGGVEAFIRANEATAERVEDITSVISVGDSIEAKYTGV
                     DRKSRVVNLSVRAKNEAEESAAIAQVNKEEVVVPNAFAEAFKAAKGE"
BASE COUNT         6235 a         3644 c         4628 g         5854 t
ORIGIN      
        1 tcttattcag gagaaacaac ggccagtcct ttattaaagt tggacacaga aagaaatata
       61 aatctgtatt ttcaagcaat acataaatgt actcctgata aaattcatac aaaaatcatc
      121 aatattccaa gtgaaggtaa tgcaaaagaa ctttggactg caacaggttg taataaaacg
      181 caatctttta ttattacata tagaaacgat ggggtaggcg gcacttttat taacatgacc
      241 acaacgccat aatttaaagg aaattacaat gacaaacatc caccaccaca aaattttaat
      301 tttagacttc ggttcacaat atactcagct tatcgcacgc cgtgtgcggg agattggggt
      361 ttactgcgag ctttgggctt gggacgtaac ggaagagcag atccgtgaat ttaacccaac
      421 ggggattatt ctttcaggag gtcctgaaag caccactgag gaaaacagcc cacgagcgcc
      481 ggagtatgtg tttaacgctg gtctgccagt gctgggcatt tgctacggaa tgcagacaat
      541 ggcgatgcag ttgggcggtt taaccgaaac gtccgaccac cgtgaatttg gctatgcctc
      601 ggtggctctc aaatctgccg atcaactttt tgcaaattta aacgatgatt tgaccgcttg
      661 cgagccaaaa ttggacgtgt ggatgagtca cggcgacaaa gtcaccaaat taccaccgca
      721 ctttaccatc acaggcgtta ccccaacctg cccgattgcc gcgatgtcgg acgaaagccg
      781 ccgtttctat ggcgtgcagt tccaccccga agtgacccac accaaacaag gcttggcgtt
      841 gctgaaaaac ttcgtggtcg gcatttgcgg ctgtgaaacg aactggacgg cagaaaacat
      901 cattgaagat gcggtgaaac gcattcgtga gcaagtgggc gatgacgagg tgattttagg
      961 tttgtcgggc ggggtggatt cctccgtcac cgccttgcta ttacatcgtg ctatcggtaa
     1021 aaacctgcac tgcgtatttg tggataacgg cttgctccgc ttgaacgaag gcgatcaagt
     1081 catggaaatg ttcggcaaca aattcggttt gaacattgtg cgagtgaatg ccgaagaccg
     1141 tttcttagaa gcgttaaaag gcttagacga accggaagct aaacgcaaaa ccattggtaa
     1201 agtgtttgtg gacgtgtttg atgacgaaag caaaaaactt agctcggtca aatggcttgc
     1261 acaaggcacg atctaccctg atgtgattga atccgccgcc agcaaaaccg gcaaagccca
     1321 cgtgattaaa tcacaccata acgttggcgg cttgccggat tatatgaagc tcggcttagt
     1381 cgaaccgttg cgtgaattgt ttaaagacga agtgcgcaaa atcggcttgg cactcggctt
     1441 accggctgaa atgctcaacc gccacccgtt ccccggcccg ggcttaggcg tgcgtgtgct
     1501 gggcgaaatc aaaaaagagt actgcgattt actgcgtaaa gccgatgcga tcttcatcga
     1561 agaactgcac aaagccggct ggtactacaa agtcagccaa gctttcacgg tattcttgcc
     1621 ggtgaaatcg gtgggcgtaa tgggcgatgg acgcaaatat gactgggtcg tcagcctgcg
     1681 tgcggtggaa accatcgact ttatgaccgc tcactgggcg cacttgccgt atgatctatt
     1741 aggcaaaatc tccaaccgca ttattaacga agtgaacggt atttcccgtg tggtgtatga
     1801 cgtgagcgga aaaccaccgg caaccattga gtgggaataa ctcttacaaa ataggcttct
     1861 tataacctaa gttcagtatc taaagctgaa aaatgttgtt aactgcttaa tgtagcggtt
     1921 tagaataaag gattgattcc aacggaatca atccttaaac taagtgcctt ttagtaaaac
     1981 taaatattca atttttagtt ttatgatttc agtaagccag atgaaccttc ggaataatct
     2041 ctaggttgat cttcttccgt tttttctgcc ggaataggta atttggtttg ttggaaaaag
     2101 gtaatatttt ttgatgcttc tggtaatagc ttttcagagc tttgtgctaa atgggtataa
     2161 agctttttat aatcagaagc tagacttgaa agtaattggg ctgattgctc aaagtgttgt
     2221 tcaagttttt gtttttgctc atccacttga gttttggttt gcttcaactc tttttccagt
     2281 tcatgctgtt ttctgacact actgttaaat actctaacta gagtgcaacc tattacagca
     2341 cctacaataa atgctgccat aatggctgcc caaacatctg ttgtccattg ttccatcata
     2401 aactccttat gttttagtga ttgattactg ctatttttaa catttcagtt tttatttgaa
     2461 aagctccaaa tgattaaaat gtgatatagg ttcaattttg aaagattatc agcaaaaaat
     2521 gttagatttt tgagtgaaat ctctgtataa tttcgccacc ctgtttttgt gtggttttcc
     2581 ccgccacaca agtttggtct gaatcagact cgaaggggtc gggttcaccg taaaataata
     2641 ggaaatacct caatgaggta ttgggtttta aactttaaat ttggtattta attaatgaaa
     2701 acttttgtag caaaaccaga aacagtacaa cgtgactggt atgtagtaga tgcgacgggt
     2761 aaaacattag gtcgtttagc gactgaattg gcacgccgtc ttcgtggtaa acataaacct
     2821 gagtacactc cacacgttga taccggtgat tacatcatcg tgatcaatgc agacaaagta
     2881 gcagtaacag gtaaaaaaca aactgacaaa atctactact ggcacactgg ctatgtaggt
     2941 ggcatcaaag atgcaacctt caaagaaatg attgctcgtc gtcctgaagc tgtgattgaa
     3001 atcgcagtga aaggtatgtt accaaaaggt ccacttggtc gtgaaatgtt ccgtaaatta
     3061 aaagtttacg caggttcaga acacaatcac caagcacaac aaccacaagt tttagatatc
     3121 taattcacgg agcataaaaa tgacagcagc aaatcaaaac tacggcacag gtcgccgcaa
     3181 aagctcttca gcccgtgtat ttatcaaacc gggttcaggt aacattacta tcaaccaacg
     3241 ttcacttgaa gtttacttcg gtcgtgaaac agctcgtatg atcgtacgtc agccgttaga
     3301 attggttgaa ttaactgata aattagacct ttacatcact gttaaaggtg gtggtatttc
     3361 tggtcaagcg ggtgcaatcc gtcacggtat cactcgtgca ttaatggaat acgatgaaac
     3421 gctacgccca gcacttcgtg cagcaggctt cgttactcgt gacgcacgtc gcgttgaacg
     3481 taaaaaagtg ggtttacaca aagcacgtcg tcgtccacaa tactcaaaac gttaagtgcg
     3541 acaagttgtc gtacttatgg caataactcc ttatatatca aggagttatt gctggaagtg
     3601 ggtggtattc ttatcacctg cttccgactt tccaagcact cttcggagtg cttggaaaga
     3661 caggcaagag aaaaggggct ttgcccaagc actcttgttc taagttggga aagtaaatga
     3721 accaactcga aaggctgtaa ggtatgatct tcactttgat gtacttggtt tttttcttaa
     3781 taccaagata gagtaagaaa gcaagatccg cagaccggag ttacccatac cccagagcag
     3841 agaaaattta caacacctac tataaataaa gtccaataga tcgcctcagc actcagaaaa
     3901 gacatcagaa ctaagacttg gtaaatctta gggaaggagt gccatcttta cttatacttt
     3961 tcacactcgc aatctacggt aatcgtagaa tgctcctcaa tagtgaataa aaacactaag
     4021 gataaactct tgtttattcg tttttatgag ctagggataa ttatacctta aattctcgtt
     4081 aaaatcacct ctaatactca cattagctag ggttagctac cttcgcctat tggacaggtt
     4141 ataggctatt tgcgggaaaa tttggatata gacaaagcga ctttagtcgc ataagtgaat
     4201 ttattttgta aaggttcgga cttatttatg cagtatagga tactgactta gtaagtatgc
     4261 gtaagctatt actgtcattg ttacggtcaa acaatccgaa gatatgagta gtacggaggg
     4321 aatgataagg taacctgtta tatggctaac agtagtctag ggatagtgcg acaccggtaa
     4381 cggtgtggtg cggagcttcc tgacaatgaa cccctgatga aaatcagtca atgaaatcgt
     4441 gagaggacat tgggagaaag tgaatagcac accttcaagg ggctagactg ggtagaaaga
     4501 taaacgtaag tgaacagttg ataaacctcg ttatcgagag agagccaaag ctattgatag
     4561 gctctgacca aaaggtaggc agtcggttat tcttctttaa cgtgaggata cgccatcaaa
     4621 gcactgtcgg gggagagact gtatcttcac tactcgtgtg atagtaatgg aacttggtaa
     4681 gcctgtattt ctgccttgcc cacaatactc aaaccgtttg aaatagatgg cgaaggtaaa
     4741 agaggaaaag gcaagtaaag cgtaagcgat actgttggga atgcaggtaa gggaggttag
     4801 aaaaagcgaa tgctatgctg taatggcata gatagggtgt gaaacatagc ctaacccgaa
     4861 agggtgcaga cttctaactg gtctttcatc gtaagatgaa tagcgtttcc tttaagggta
     4921 acaccgaaag agattgtaga taacagaaga gaaattgaat gttatcaagg aatacaatgt
     4981 tgaataggta caaaaacaaa ttaaagaaat gagcgttagg ctcatcataa cctagcgctc
     5041 gactctttaa cccaatcggt tacaagagcg gttgaaatta tcggtaaaac ggtaagatcc
     5101 tcttgtaacc aaagggaata aactaaggat agtttatctc aatagggatt tgtttacttg
     5161 aaggatctac ccacttgctg agactttcga cttatcttgt aatatcagag gtaggtatga
     5221 aagagcgtaa gcattatact gaaaagttag cttgcaatcc agcgacctcg cacatctcca
     5281 aatggcatta tgtcgattgg cataaagctg aaacgtatgt gaaaggaatg caaattcgga
     5341 ttgcgaaggc aacactggaa ggcaattggc gtaaggttaa aaacttacaa cgaatgctaa
     5401 cccactcaca ttatgctaaa ttagttgcag taagaagagt gactgaaaat caaggtaagc
     5461 gaacaagtgg tgtcgataaa gagctttggg agaccccaga gacaaaatgg caagccgcac
     5521 tatcattgac ggaaaaaggg tataaaccta gtccattaag acgagtgtat atccccaaac
     5581 cgaatggaaa gaaacgtccg ttgggtattc caacaatgcg agatagagca atgcaagccc
     5641 tttatttatt ggcaatacaa cctgtggcag aaacaactgc ggataaaggt tcttatggat
     5701 ttagattaaa ccgttcaacg gtagatgcta ttactcatat acactaaatt tattcgcata
     5761 aaggcaagaa aactaatcaa cctgttgagt gggtacttga tgcggatatt gcaggttgtt
     5821 ttgaccacat aagccacgac tggctattaa agcatattcc aatgaataaa cggatattgc
     5881 ggaaatggct aaaatcaggt gtagtggaat ttggacggtt gaaacgaaca gaggaaggta
     5941 caccacaggg tgggattatc tcgccaacgc ttgcaaatat ggcattagat ggattggaaa
     6001 aactgttaga aactcatttt ggaggaaaag gcagtcggac aattcgagaa aataaaactt
     6061 acttagtacg ttatgcagat gactttatca tatcaggtaa aacgaaagag ttacttgaaa
     6121 atcaagttat cccacttatc cagaactttc tgaatgaacg aggtttatcc ttatcaacag
     6181 aaaagacgaa agtggtacat attgaagaag gttttgattt tctaggttgg aatgtgagac
     6241 gtttcaaagg aaaaatactg aacagaccaa gtcaaaagaa tgtgaaagcg ttctacaaca
     6301 aggtaaaaga ggtaatttct aaatgaaaat ggctaaacag gaagatctta taatggtgct
     6361 aaacccaatg ttacgaggtt gggcaaatta tcatcgaagc caagttgcaa gtaaggcatt
     6421 cggacgaatg gacgcattag tttggaaagt actttggaaa tggtgtgaac gccgtcatca
     6481 caataaaggt attatgtgga taaaagagaa atacttttgt accacagcga ctcgaaattg
     6541 ggtatttggc acagtttata ccaatgaaaa aggagaaact aagccgatag acttattcta
     6601 ttgtggctat gttaaaatca aacgacacgt taaaattaag tctgaatata atccgttttt
     6661 gcctgaatgg gagttgtacg gcgaaaaact gagccaagaa cgtctgtatg aagaacaatc
     6721 acacagacga caatggcagg cactgtataa agaccagcgt ggaaaatgtg cattatgtgg
     6781 gttgccaata accaaagaaa caggctggca cgatcatcat atcgtgtaca aaatgttagg
     6841 aggaagcgat gcattaagca atcgttgttt ggtacatccg acttgtcaca taaagattca
     6901 tactttgaac ttggaggtcg tgaagccggc tatatagctg atgaaaggct tgagctgtat
     6961 gcagagaaat ttgcttgtac agttcttagg gggcggaggt gcagtaatgt actttcgcta
     7021 cccgactttt cttttcgttt tcaagaaggc agggtttcct gccttttttg tctttatgac
     7081 tgtggtagaa taaccgccct gattaactat ttgatagagg catttatgtt agccattatt
     7141 tcccctgcca aaacccttga ttttaaaacc aatattgacg gttttgcatt ttctcaacca
     7201 aatttgaccg cttgtagcca agaattaatt gatatttgta aacagctttc acctgctgaa
     7261 ttaggtagtt tgatgtcaat tagcgataaa ttagccgctt taaatgtggc tcgctttgcc
     7321 gagtggcagc ttgagcataa cgaacaaaat gccaaagccg ccctctttgc ctttaaaggt
     7381 gatgtttata ccggactaga ggcagaaacg ttaagcaaag cacaagttga atatgctcaa
     7441 caacatttgc gtatgctttc aggcttgtat ggtttattaa agccgttaga tttaatgcag
     7501 ccttatcgtt tagaaatggg cactaagtta gccaatccga aaggcaaaga tctttatgct
     7561 ttctggggca atattattac tgaaaatttg cagcaagcga ttgatgaaca aggcgatgat
     7621 attttagtta atcttgcctc tgatgaatat tatggtgcgg ttaagccaca aaatctaaat
     7681 gcgactatta tcaagccggt gtttttagat gagaaaaacg gtaaatataa agtgatcagc
     7741 ttctatgcca aaaaagcccg tggaatgatg gttcgcttta tattagacac ccaaccgact
     7801 agcatcgaac agctcaaaca gtttaactac gggggctatt ggtttgatgc agacagttct
     7861 agtgaaagcg agctagtctt taaacgtgag gagcaggcat aatgtcggct atcaaaaagg
     7921 gaataacttt ggttgcgtta tcattggcag gttgtgtcag ccaaagccca caatttgcag
     7981 tgcctgatgc tattcacttt gggcaggata cttttgtaaa agtcacccac agccaaattg
     8041 atgaaatgca acatttactt tatttgccag aaaagagtga gaaaaatccg gaaaattggc
     8101 aaaaaggcat tttatttttc ttagataaaa acagtaaaaa tcaaacctta gagcaacgag
     8161 ctgcattccg ccaagcaagt tttgcgaaaa agccggagct tgaggcgaaa gtggaaattc
     8221 agcaaaatga attacacagc agtgtgattt atccacctat agaacgtttt aacaatgtga
     8281 tgttagaagt aacccgtggg cggaatttag cttgtggtta cgggcagatt caatttgcag
     8341 ataagcggga agttggcaag acagttgcaa aaaaatcgcc gaatttgacc gcttacagcc
     8401 aagaaattgc tagactttct cttgaattaa accaactggg ttggcaagtc caatgcagta
     8461 aataatgtag taaatgaacg aaaaatacag caaattagtc aaaaatgcag cgaatttagc
     8521 gattattgtt gcgatcttac tgattgtgat taaagccttt gcatggtgga aaaccggttc
     8581 gatttcaatc ttggcagcaa tgacggattc ggtagtggat ctgtttgctt cgctaaccaa
     8641 tattttagtg ctgcgttttg cactacaacc ggcagatgag aatcacgcgt ttgggcacgg
     8701 caaagcggag tctttggcgg cacttgccca aagtgcgttt atttccggct cggcggtttt
     8761 cctactgcta caaggtttcc ataaactcac caatccggaa ttaattgctc aatcgagtat
     8821 tggggtaatt gtcagtattg tatcaattgt gattactgca cttttagtct tttatcagcg
     8881 ttatgttgtc acgcttacga aaagtccggc gattgaagcg gattcgttac attaccaaac
     8941 tgatttatta atgaatgcgg caattttagt ggcaatgttg cttaatagtg ccggttttgt
     9001 gtacgccgat gcagtttttg caataggtat tgccctctac attgctttca atgcagtaaa
     9061 aatgttttgg tgtgcggtgc aaaccttgct agaccaagct ttgcccgatg aagaaattga
     9121 gcggattaaa gagattgctt cgcaacatcc gaacatttta gggattcacg atattctaac
     9181 ccgccgtgca ggtgcagtgc gttttatcca attacatttg gaattagacg atcacttaac
     9241 gctgctagta gctcacgaaa taactgatag ccttgagcag aaattgctgg cggcatttcc
     9301 gatgtctcac gtgattattc atcaagagcc gactacggtg gtcaaacaag aatttcaaga
     9361 tatggcggag aatatttaat gtatcaggcg atagtgcatt tcagctatca taattttgag
     9421 caagatccgg tgacggcaat tagccaaatc ttaaatcaat ggttatataa cgggcaagtc
     9481 attggcagag aaatgccgat tacttttcat caaaacgagt ttcaagctcg ggtttgtgtg
     9541 cctacacaag aaagcctttt gccacaatat aatagtgatg aagtaaatga agcgttgcaa
     9601 caagcggtcg aaatcggcgt aatttttgca agttttgagc ttgtcgggcg agattatcag
     9661 ggcgaagaaa gcagtcataa ccgctcgcca gcgtttcaaa tattatatac cacccattta
     9721 gatacctgct cgccacttta tgatggagag cagtttgccc caattccgct ttattcttta
     9781 caggatcagt ctcttagcga agcgatattg gaatggcagc aaaattggca agcgtgcgat
     9841 ctgctacaaa tgaacggcaa tctgctggag tgccacgcac ttgttcaaat tagtgatgaa
     9901 acaagcgatc ttgcaatgat cggtcgagat ctatgtcagc aaattgagca gaaaactcac
     9961 attcccacct tttattacct ctaccgttta gggcgtgatg aaagtgaata tcaacgcaaa
    10021 tgcccttgtt gtggcggaga atggaaatta actgagccgt tgcacgatat tttccatttt
    10081 agatgtgagc cttgccgttt gatctctaat ctaagctggg aattattata acttttagtt
    10141 ataccaaagt aataaatcat cttgaatgaa tgccgcaagc gtaataccct tgcggcattt
    10201 ttataataag cggttttact ttggaggttc tgtgcttatt ttcaacttaa ttgtctttgc
    10261 ggcaatatta tttttattgg ctaaaattta taaatctacg caaaaactcg gtcaaacagt
    10321 gtttattggg ttatttttag ggcttgcaag cggtgctttt ttgcaaactt tttacgaaaa
    10381 agctactgtt gatgcaactc ttgattggat taacttaatt ggtaacggtt atgttcgctt
    10441 gctacaaatg atcgtaatgc cattggtgtt tgtctctatt ttatcggcaa ttacccgctt
    10501 aaaagaggca ggatctttag gcaaaattag ttttagtgtg ctatcagtgt tattagtgac
    10561 caccgcgatt gccgcagcga ttggtattgc tttagtgtat ctgtttgatt tatctgccga
    10621 gggtttagtc gccggctccc gtgagcttgc cgctcaagag aaagtggtag ggcgtgtgga
    10681 gcaggtgagc aacttatctg tgccttcaat gttgctttct ttcattccga aaaatccgtt
    10741 tttagaatta accggtgcaa acccaacgtc gatcatcaac acggttattt tctcggctct
    10801 gctaggcatt gctgcactga gcttaggcaa agaagataaa gcgttgggcg aacgtatcgc
    10861 acaaggtgta gagaccttaa ataaactggt tatgcgttta gtacgttttg tgattcgcct
    10921 aaccccttat ggcgtatttg cgttgatgat caaaatggcg gcaacttctc aatggagcga
    10981 tattgtaaat ttaggtagct ttattgtggc atcatacatt gcgattggtt tgatgtttat
    11041 tgtgcacggt attttactct ttgtggctaa aatcaacccg ttggattact acaaaaaagt
    11101 gttgccgaca ttaagttttg cttttacgtc tcgttccagt gcggcaacca ttccgttgaa
    11161 tattgagacg caaaccaata aattaggtaa taacagtgtg attgccaact tttctgcaac
    11221 ctttggggca accatcgggc aaaatggctg tgcgggaatt tatccggcaa tgttggcggt
    11281 gatggtcgct ccaacagtgg gaatggatcc gtttagctta aactatattt taaccttgat
    11341 tttggtggtg gcaatttcct catttggcat tgccggtgta ggtggtggtg caacttttgc
    11401 ggcgattgtg gtgctatcaa ccttgaatct gccgattgag ttagtaggct tgctcatttc
    11461 agttgagccg attattgata tggctcgcac cgccttaaac gtgaatggtg caatggtggc
    11521 gggcacatta accaataaat gggtgaaagc cgagtagtca aaactaaaga tataaaaatg
    11581 ggcgattaat tcgcccgttt ttataagtta aggtgattat tcaatccacg caatcacttc
    11641 ttcggtaggt ttgcgggatt ttttcttaat ttctttgttg cgatagccaa aagttatcat
    11701 acaagacaca ccatattcgt ttaggtcaaa taagccttcg tccgataaaa tttggtttac
    11761 ggtatcgtaa tgaaaacctt caatcgggca agagtcaata ccaatcagag ccgcaccggt
    11821 catcatattg cctaatgcaa tataggtttg cttggtactc caatcgaata attcacgttc
    11881 actaagcaat ttcatatcct cttcttggaa cgacttatag cgtaccagcg aggcttcaat
    11941 ctgctcatag gttaagcctc gtttggctaa catatcacgc atatagacag aatcataacg
    12001 gacattcttt ttggttaaaa tcaccactaa atggctcatt tcttccattg ggtgtttaat
    12061 tccccacgaa accggggcga ttttctgacg ggtttccggg ttttgtagca ctaaaaattt
    12121 ccacggttca gaaccgacag agcttggtga gagacgcccg agttccaaaa tataatccat
    12181 atcttctttg ttgatttttt tgtttggatc gtaactgcga caggccgaac ggaagtgaaa
    12241 agcgtctaaa acttgctgtt tgctaatatt aaacatcgtt tttcctttta taagtgaaat
    12301 aagtagatta atgataactt gatttagagc agaatgcgaa tttcacgcct gttaatttgc
    12361 aaaaaattag gaaaaaatga ccgcttgttt aaaattgctt taccttaaaa atcaatcatt
    12421 taacgaatcg gttgatttac tttttctggc taaagtatta ttattcatga aattttttat
    12481 ttcattgagt ttagaggata ggtaatgcaa cacctaaaag atttaactaa taaagcacgt
    12541 gaagctttag ataagattga tcaaaattta gaggctttag aagagtttag agtcgaatat
    12601 tttggtaaaa aagggcattt tacaacgtta atgcaagaat tacgcaatat tgcagcggaa
    12661 gaaagaccgg caatcggagc aaaaattaat gaagcaaagc aagttgtgct tgatgtgtta
    12721 aatgccaaaa aagaagaatg ggaacaagct gtattaaatg caaaattagc taatgagagc
    12781 attgatgttt cactgccggg gcgtaaaact gaattaggtg gtttgcaccc tgtttctatc
    12841 actattgagc gtgtggtgaa atttttctct gaattaggtt ttaccgtagc aaacggacct
    12901 gaaattgaaa ccgattacta taacttcgat gcgttaaaca ttccggatca tcatccggct
    12961 cgagcggatc acgatacgtt ttggtttgat gccaatcgtt tattaagaac ccaaacctcg
    13021 ggtgtgcaaa tccgtacaat ggaaaatgta aaaccgccta tccgtattgt ggcaccgggg
    13081 cgtgtatatc gtaatgacta cgaccaaacc cacacaccga tgtttcacca aatcgaattg
    13141 ttatatgtgg ataaaaaagc aaactttacc gagttaaaag gcttaattca cgattttctc
    13201 aaagcattct ttgaagaaga tttacaagtg cgtttccgcc cgtcattctt cccattcact
    13261 gagccttctg cagaagtaga tgtaatgcgt caaaacggca aatggttaga agtgttaggt
    13321 tgcggtatgg tacacccgaa tgtgttacgc aatgtaggga ttgaccctga agaatatagc
    13381 ggttttgctg tgggaatggg cgtcgagcgt ttaacgatgt tacgctacaa tgtcacggat
    13441 ttacgttcat tctttgaaaa tgacttacgt ttcttaaaac aatttaagta atacaagcgg
    13501 ttaaaaattt agaaaatttt acaaatagga tagaaataat gaaattcaat gaatcttggt
    13561 tgcgtgaatg ggtaaaccca gcaattagca cagaacagtt atgcgaccaa attacaatgt
    13621 tagggttaga agttgacgat gtagagccgg tagctggtgc gttttcgggt gtagttgtgg
    13681 gtgaagtggt ggaatgcgcc cagcacccgg atgctgacaa attgcgtgtc actaaagtga
    13741 atgttggtgg cgaacgttta ttagatattg tttgtggtgc accaaactgc cgtcaaggtt
    13801 taaaagtggc ttgcgctgtt gaaggtgcag tgctaccggg cgatttcaaa attaagaaaa
    13861 ccaaattgcg tggtcagcct tcagagggga tgttatgttc ttactcagaa cttgggatta
    13921 aagaagatca tagcggtatt attgaattac ctgctgatgc acctattggt caagatttcc
    13981 gtgaatattt aaatttaaat gatgtggcga ttgaaatcag ccttacgcca aaccgtgccg
    14041 actgcttaag cattgccggc attgctcgtg aagtgggggt gattaaccgt gtcgatgtga
    14101 aagctccaac tattacagat gtaaaagcca cgatttcaga taaagtttca gtggaattac
    14161 aagcaccgga ggcttgccct cgctatttag cccgtgtggt gaaaaatgtg aatgtcaaag
    14221 cgacatcgcc actttggtta caagaaaaat tacgccgttg tgggattcgc tcaatcgatc
    14281 caatcgttga tattactaac ttgagccttt tagaattagg gcagccaatg cacgcctttg
    14341 atgcagcgaa aattgaaggt ggcattcaag tgcgtatggc aaaagaaggc gaagaattgg
    14401 tattgctaga tggcacaaac gctaaattac aatcgaatac cttagtgatt gctgatagca
    14461 aaggggcatt ggctatggca ggtattttcg gtggcgaagc cagcggggtg agcgagaata
    14521 cgaaagatgt ggtgttagag tctgcatttt ttgctccatt agcgattacc ggtcgcgctc
    14581 gtcaatatgg gttacatacc gacgcatcac accgttttga gcgtggggtt gatccagaat
    14641 taactcgtca agcaatggag cgtgcaacgg cattattact ggaaatttgc ggcggtgaag
    14701 caggtgaaat tgtggaagcc gtgagccaag aaaacctacc aaaacgcaat actgtcacgc
    14761 ttagacgcag taaattagat tcggtgattg gtcatcatat tgacgatgca acggtaaccg
    14821 atattttaac ccgcttaggc ttgaacgtaa catttgcgga taatagttgg acggcaattg
    14881 ctccaagttg gcgttttgat attgaaattg aagaagattt aattgaagaa atcgcgcgaa
    14941 tttatggcta caacagtatc ccaaataatt cgccacttgc tcatttaacg atgaaaggta
    15001 cgccggaaaa attactggaa gcaaaccgta ttcgtatggc attggtagat agtgattacc
    15061 aagaagtgat tacttatagc tttgttgatc cgaaaaaaca atcattattg cacccacagc
    15121 aagaagcctt gattttacca aacccgattt caagtgaaat gtcggcaatg cgtgtttctc
    15181 ttttaaccgg tttgttggac actatcgtgt ataaccaaag ccgtcagcaa aaccgtgtgc
    15241 gtatttttga agcaggtcta cgttttattc cggatagcaa tgcggaatca ggcgtgcgtc
    15301 aagaatttgt ttttggtgcg gcgattgtag gcgataaacg tccggtacat tgggaaaaca
    15361 aatcagaagc agccgatttc tttgatttaa aaggcgatat tgagcgtatt ctttctttaa
    15421 cctctatccg ccacgatctg caatttgtcg caaaacagtt cccagcatta cacccggggc
    15481 aatcggctgc gattatgctt gatggcaaag aaattggttt tatcggaact gttcacccgt
    15541 caattgtgca aaagtttggc attaaaggca aaccgattgt atgtgaaatt ctagcatcag
    15601 ctattgcaga gcgtccggtt acacaagcgg gcgaaatttc taaatttcct gcaaataacc
    15661 gtgatattgc ggtagtggtt gatgaatctg ttcctgcagg tgaggtgtta gaggcttgtc
    15721 gtaaagctgg tggtaataaa ttagtggcag taaatttatt tgacgtttac cgtggttcga
    15781 atttagaaag tggtaagaag agtttagcca ttagcttaac ggttcaagat aatgaaaaaa
    15841 cattagaaga aaatgaaatc aatgcggtaa ttcaagcggt attagctgaa ttagcaacac
    15901 gtttcaatgc ggtacttcgt gattaatcag taaaatatgg ctgatttatt ttatcagcca
    15961 taacaataaa ctaaggataa atgatggcgc ttaccaagat tgaaattgca gaaaacctga
    16021 ttgaaaaatt tgggttagaa aagcgtgtag cgaagcaatt tgttgaattg ttctttgaag
    16081 agatccgatc ctccctcgaa aatggtgaag aagtgaaatt atctggtttt ggtaattttt
    16141 cactgagaga aaaaaaagct cgtccaggaa gaaatcctaa aacgggggaa aatgtggctg
    16201 tttcagcccg tcgagtagta gtttttaaag ctgggcaaaa attacgagaa cgtgttgagc
    16261 aagctagctc ccaatcatag gggcagttat attagttaga tttaatgtgg agtaataaat
    16321 gaaattaacc aaacggtggc tttccttagg ttgtttgtta gctattacag cgatgttagt
    16381 ggcttgttca agttcagata caacctctaa gtctcaagta aaaagtaaag tttatcgttc
    16441 aatataccaa tcagaccttt ctgatcctat tatggcaatt agtcgattaa gtgaacatca
    16501 acacgaatgg aaaggcactc gctatcgttt aggtggcaat agcaaggctg gcatcgactg
    16561 ttctggtttt atgcaagtca cttttaaaga tctttttggg atagatttgc cgagaaccac
    16621 cacagagcag gctaaggctg gtgagcgtat atccaaagac gaattgcgta caggtgattt
    16681 agtttttttc aagacagggc gtggtccaaa tgggaaacac gttggtgttt atgtaaaaaa
    16741 tgggcaattt cttcacgcct caacaaaggg aggggtgatt tattctgatt tggattcgcc
    16801 atattggtct aaggctttct ggcaggctcg tcgtttataa tttagggtaa catacatgct
    16861 gaaaaaaatc ggacaagcgg tcatatttgg acttttttgt gcaattttaa ttttgtttgt
    16921 agctccaatt attcagagta aagggagttc gttttcatta tcagcattca ctcaaaaaga
    16981 gattgtaagc tacaatgcag cggttaaaat agcctccccc gcagtcgtta acgtatatag
    17041 ccgaaatttt agttctgaaa atgaaacatt tgaagttaag aatttaggtt cgggtgtgat
    17101 tatgagtagc gatggctata ttttaactaa caggcatgtg attgaagatg cggatcaaat
    17161 cgtgattgct ttgcaaaacg gtgctatttc gactaatgta aaattagtcg gcgatgattc
    17221 tttaactgat ttagctgttt taaaaattga gggtgagaat ttacctacta ttccacaaaa
    17281 tccggagcgc aaactacaaa ttggcgatgt ggtgttggca attggtaatc ctttgaattt
    17341 aggacagagc attacgcaag ggatagtcag tgctattggg cgaaaaacct taacagaaag
    17401 tggtagacaa aattttattc agacagatgt ttccattaat caagggaact caggtggtgc
    17461 tttaattaat actgctggtg agctaatagg tattaacact ttgcgttttg ggaaaaatac
    17521 gaatgaattg tcagagggcc taaattttgc gattcccatt aatctggcga atcaaattat
    17581 gagtaagatc attaaagatg gccgagttat tcgtggctat tttggggtaa gtacagattt
    17641 gcttgacacc gcaaaaatgc aaggttatga aaaaggggtg atcattgtta atgtagataa
    17701 aaatgggcca gccgcaaaag cggggattca agcaggtgac ttagtgctta aagtcggaga
    17761 tattgaggca ttctctcctt ctcaaatgat ggaagagctt gctgaaatga aaccttatac
    17821 tacagtaaaa gtattggttc aacgtgatga acaattactt aactttgatg taactattac
    17881 tgagttacag actcaatagg gtagaaaatg aaaaaaatag tgattacagt tgatgggccg
    17941 agtggtgcag gtaaaggaac gttatgtcat gcattggctg aaaaattagg gtttgatttt
    18001 ttagattctg gagccttata ccggattact gcattaagcg caattaaaaa agaggttccc
    18061 cttgatgatg aggaaaaact agcggaagtt gggcgtaatc ttgatattca atttttgcca
    18121 gaaaatggcg agattcaggt gattttggaa ggagaaaatg taggcgactt aatccgaacg
    18181 gcagaagcag gtcaaaatgc ctctaaagtc gctgctttcc ctttggtacg tgaagcactt
    18241 ttagaacgcc aaagagcctt tagtagcgaa aaaggtctga ttgcagatgg acgagatatg
    18301 gggacagttg tgtttcctga tgcacaaatt aagctgtttt tagatgcaag tgccgaagaa
    18361 cgcacaaaaa gacgtgtaaa acagttgcaa gaaaagggat ttaatgctaa ctttgacgag
    18421 attttagccg agataaaaga acgtgatttt cgcgatagaa atcgatcagt cgcgccactt
    18481 gttccggcta aagatgcgtt gctgttagat tcaacgcatt tatcaattga agatgtgatt
    18541 aaccaagcat taaatcatat cgctcaatac attaaatttt agtttgttgc ttcacatagg
    18601 atttgtgtag cttttattat caaccccgtt taacaggacg ttagatggat gtttattttt
    18661 aagaagattt attatgtcag aattatcgtt tgctcaacta cttgaagaat catttgcagc
    18721 tacaccacgt ttaggtgatg ttgttaaagg tactgttgtt gctatccaaa aaggcttagt
    18781 tattgttgat acaggtttca aatctgaatc tgcaatcgat gcctcagaat ttactaacgc
    18841 acaaggtgaa ttagaagttc aagtgggtga tgaagtagat gttgttctta aagctgtaga
    18901 agacggtttt ggtgcaacag tagtttctcg tggtgatgca aaacgtaacg aagcttggat
    18961 tgctttagag aaagcatttg aagaacaagc tacagttatc ggtttcgtaa atggtaaagt
    19021 aaaaggcggt ttcactgttg aattaaacgg tgttcgtgca ttcttacctg gttcattagt
    19081 tgatactcgt ccggttcgtg attctttaaa cctagaaggt aaagagtacg aattcaaagt
    19141 aatcaaatta gatcaaaaac gtaacaacgt agtagtatct cgtcgtgctg ttatcgaaac
    19201 agaaagcaac caagatcgtg atgcggtatt agcaaactta gttgaaggtt ctgaagttaa
    19261 aggtacagtt aaaaacttaa ctgactacgg tgcattcgta gatttaggtg gcgttgacgg
    19321 tttattacat atcacagata tggcttggaa acgtgttaaa cacccaagtg aagtggttaa
    19381 tgtaggcgat gaagttactg ttaaagtatt aaaatttgac aaagacaaaa ctcgtgtatc
    19441 attaggctta aaacaattag gtcaagatcc ttgggctgta attgctgaaa accacccggt
    19501 aaacagcaaa ttaactggta aagtaactaa cttaactgac tacggttgct tcgttgagat
    19561 tttagacggt gttgaaggtt tagttcacgt ttcagaaatg gattggacaa acaaaaacat
    19621 tcacccatct aaagtggtta acgtaggcga tgtagttgaa gtaatggtac ttgaagttga
    19681 tgaagaacgt cgtcgtatct cattaggttt aaaacaatgc aaagcaaacc cttgggaaca
    19741 atttgcggca actcacagca aaaatgataa agtatcaggt aaaatcaaat caattactga
    19801 cttcggtatt ttcatcggtt tagaaggtgg tattgacggt ttagttcact tatctgacat
    19861 ttcttggaat gtttcaggtg aagaagcagt tcgtaactac aaaaaaggtg atgaagtaga
    19921 agctgttgtt ctacaagttg atgcagtgaa agagcgtatc tctttaggta tcaaacaatt
    19981 agaatctgat cctttcacaa actttgtaga tggtactaaa aaaggtgcaa tcttaacagg
    20041 taaagttgta gaagttgatg ctaaaggcgt taaagttgaa ttggaaggtg gcgttgaagc
    20101 atttattcgt gcaaacgaag caactgctga gcgtgtagaa gacatcactt ctgttatttc
    20161 tgttggcgat tcaatcgaag cgaaatacac cggtgttgat cgtaaatctc gcgtagttaa
    20221 cttatctgtt cgtgcaaaaa acgaagctga agaatcagca gcaattgcac aagtgaacaa
    20281 agaggaagtt gttgtaccta atgcatttgc tgaagctttc aaagcagcta aaggcgaata
    20341 attagtatag actaatctaa a
//
LOCUS       DANLZC010000027        20331 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.027, whole genome shotgun sequence.
ACCESSION   DANLZC010000027 DANLZC010000000
VERSION     DANLZC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20331
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.027"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(66..1415)
                     /locus_tag="PXE44_000798"
     CDS             complement(66..1415)
                     /locus_tag="PXE44_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL5588329.1"
                     /translation="MLLFIMFAAIILLLVLIMKFKVHAFVALIIVSLLTALAAGIPVD
                     KILPTLLNGFGSTLASVALLVGLGAMIGRLLEITGGAKVLADTLINKFGKERAPFALG
                     VASLLFGFPIFFDAGLVVMLPIIFSVAKQFGGSVLRYAFPSAGAFAVMHAFLPPHPGP
                     VASGDLLGVNMGLLVLVGLVCAIPTWYIGTYLFSQFISKRIHVDLPKAFLNGLSANEM
                     AVQNPPSFGKVLTVLVLPIILILFDTGLNTLSVAKVIDGSEMWVQSLRLIGKTPVALL
                     ITLIVAIMLLRDNRSFDQIEKICNNALGPICSIVLVTGAGGMFGGVLRASGIGDVLSE
                     MMKDTGMPIVVAAFIIATVFRVAQGSATVALTTTAALIAPTVAAATDLSQFDLCFIVI
                     AIASGATVLSHVNDSGFWLMSRFLEMDEKTTLKTWTMLETAIGLTGFACALIGSWLL"
     gene            1602..2117
                     /locus_tag="PXE44_000799"
     CDS             1602..2117
                     /locus_tag="PXE44_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL5588330.1"
                     /translation="MANGKAFILMGVSSTGKTSVGTAVAQRLGIKLIDGDDLHPRANI
                     LKMASGTPLNDADRTPWLERINDAVFSFEQKSEKGIIVCSALKKKYRDQIRQGNADVT
                     FLFLHGSFELVLARMQQRKGHFMKPEMLQSQFNTLEIPQADEPDVIFIDIDGSFDEVV
                     ERCVKAVEPLL"
     gene            complement(2171..2695)
                     /locus_tag="PXE44_000800"
                     /pseudo
     CDS             complement(2171..2695)
                     /locus_tag="PXE44_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(2699..3859)
                     /locus_tag="PXE44_000801"
     CDS             complement(2699..3859)
                     /locus_tag="PXE44_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDL5588331.1"
                     /translation="MIEQSYKIALLFNANKIYDRGVMQGIGQFIQASQCTWDIFVEDD
                     FVYHKNSIESLSIDGIIADFDDLETVELLKDIDVPIVAVGGSYQNPDYYPDLPYVATD
                     NYALVEAAFLHLKQKGINQFAFYGYPAETEKHWAIERKNAFIQLMEKYEHHPNCYIGD
                     QFHSDNWVELQAKLGEWLQTLPAHTGIIAVTDARARHLLQTCEHLKIAVPEQLCVIGI
                     DNEELIQYLSRVSLSSVTQGTSQIGYHAAKLLYRKLNNLPVSTKPILIPPVKVEERRS
                     TDYRSLQDPMVIQAMHFIRHRACQGIKVEQVLDHLKISRSNLEQRFKQEMNKTIHQVI
                     HEEKLARATYMLKFTEISTQEIANVCGYPSLQYFYAVFKKEFGKTPKEFRQE"
     gene            complement(3982..4368)
                     /gene="rplQ"
                     /locus_tag="PXE44_000802"
     CDS             complement(3982..4368)
                     /gene="rplQ"
                     /locus_tag="PXE44_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL5588332.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMASALVEHEIIKTTLPKAKEL
                     RRVVEPLITLAKADSVANRRLAFARTRNVDTVAKLFNELGPRFAQRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRPEVATEAAAE"
     gene            complement(4404..5393)
                     /gene="rpoA"
                     /locus_tag="PXE44_000803"
     CDS             complement(4404..5393)
                     /gene="rpoA"
                     /locus_tag="PXE44_000803"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL5588333.1"
                     /translation="MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILEVLLNLKGLAVKVQNKDDIILTL
                     NKSGIGPVTAADITHDGDVEIVNPDHVICHLTDKNASINMRIRVQRGRGYVPASARLH
                     TQNDDRPIGRLLVDARFSPVDRIAYNVEAARVEQRTDLDKLVIEMETNGTLDPEEAIR
                     RAATILAEQLAAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAETIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIAED"
     gene            complement(5424..6050)
                     /gene="rpsD"
                     /locus_tag="PXE44_000804"
     CDS             complement(5424..6050)
                     /gene="rpsD"
                     /locus_tag="PXE44_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL5588334.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIESKCRNRLDVAPGQHG
                     ARKPRLSDYGSQLREKQKVRRIYGILERQFRNYYTEANRLKGNTGENLLVLLEGRLDN
                     VVYRMGFAATRAEARQLVSHKSIVVNGRVVNIPSFQVSVDDVVAVREKSKKQARIKAS
                     LELATQREKPTWLEVDATKMEGVFKRRPERSDLSADINEHLIVELYSK"
     gene            complement(6079..6468)
                     /gene="rpsK"
                     /locus_tag="PXE44_000805"
     CDS             complement(6079..6468)
                     /gene="rpsK"
                     /locus_tag="PXE44_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL5588335.1"
                     /translation="MAKTPVRARKRVKKQIADGVAHIHASFNNTIVTITDRQGNALAW
                     ATAGGSGFRGSRKSTPFAAQVAAERCAEMVKEFGLKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(6485..6841)
                     /gene="rpsM"
                     /locus_tag="PXE44_000806"
     CDS             complement(6485..6841)
                     /gene="rpsM"
                     /locus_tag="PXE44_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL5588336.1"
                     /translation="MARIAGINIPDQKHTVIALTAIYGIGKTRAKAICAATGIAEDVK
                     IRELSEEQIEKLREEVGKFTVEGDLRREVTLSIKRLLDLGCYRGLRHRRSLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(6979..7092)
                     /gene="rpmJ"
                     /locus_tag="PXE44_000807"
     CDS             complement(6979..7092)
                     /gene="rpmJ"
                     /locus_tag="PXE44_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL5588337.1"
                     /translation="MKVRASVKRMCRNCKVIKREGVVRVICSDPKHKQRQG"
     gene            complement(7117..8436)
                     /gene="secY"
                     /locus_tag="PXE44_000808"
     CDS             complement(7117..8436)
                     /gene="secY"
                     /locus_tag="PXE44_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL5588338.1"
                     /translation="MAKQPGYQGSTQKGTGELKSRLLFVLGALIVFRIGSFIPVPGID
                     ASVLSELIRQQQGTIIDMFNMFSGGALSRASIFALGIMPYISASIIIQLLTAIHPPLA
                     ELKKEGEAGRRKISKYTRYGTLLLAFIQSIGISLALPNMVHGLVPNPSILFYVTSVVS
                     LVTGTMFLMWLGEQITERGIGNGISLIIFTGIVADLPATIGQTIEQARQGELSFIVLL
                     LVAIIAFAVTYFVVFVERGQRRIVVNYASRQQGRMMAPARTSHLPLKVNMAGVIPAIF
                     ASSIILFPASITQWFGQSEGFEWLFNLSQLLQPGQPLYVVLFTMAVIFFAFFYTGMQY
                     NPRDTADNLKKSGAFVPGYRPGEQTSRYIDKVMTRLTLIGALYIAFVCLVPYIITSLW
                     KVPFQLGGTSLLIVVVVIMDFIAQVQSHLMSQQYDSVLKKANLKGLN"
     gene            complement(8439..8873)
                     /gene="rplO"
                     /locus_tag="PXE44_000809"
     CDS             complement(8439..8873)
                     /gene="rplO"
                     /locus_tag="PXE44_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL5588339.1"
                     /translation="MRLNSLSPAEGAKHSAKRLGRGIGSGLGKTGGRGHKGQKSRTGG
                     GVRRGFEGGQMPLYRRLPKFGFTSLKSFHVAEIRLNDLAKVDGNEVTLETLKAANIIT
                     KDILSAKVILAGKIERAVVVKGLRVTKGAKAAIEAAGGSIEE"
     gene            complement(8878..9054)
                     /gene="rpmD"
                     /locus_tag="PXE44_000810"
     CDS             complement(8878..9054)
                     /gene="rpmD"
                     /locus_tag="PXE44_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL5588340.1"
                     /translation="MAKIKVTQTRSSIARLPKHKATLKGLGLRHIRHTVELEDTPAVR
                     GMINQVSYMVKVEE"
     gene            complement(9061..9561)
                     /gene="rpsE"
                     /locus_tag="PXE44_000811"
     CDS             complement(9061..9561)
                     /gene="rpsE"
                     /locus_tag="PXE44_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL5588341.1"
                     /translation="MSNIEKQAGELQEKLIAVNRVSKTVKGGRIMSFTALTVVGDGNG
                     RVGFGHGKAREVPAAIQKAMEKARRNMINVALNEGTLQHPVKGSHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVRNVLSKAYGSTNPINVVRATIDALENMKSPEMVAAKRGKT
                     VEEILG"
     gene            complement(9576..9929)
                     /gene="rplR"
                     /locus_tag="PXE44_000812"
     CDS             complement(9576..9929)
                     /gene="rplR"
                     /locus_tag="PXE44_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL5588342.1"
                     /translation="MDKKVARIRRASRARHLMREQGATRLVVHRTPRHIYAQVIAPNG
                     SEVLAAASTVEKVIKEQVKYTGNKEAAAVVGKIVAERAIAKGIKAVAFDRSGFKYHGR
                     VQSLADAAREAGLQF"
     gene            complement(9944..10477)
                     /gene="rplF"
                     /locus_tag="PXE44_000813"
     CDS             complement(9944..10477)
                     /gene="rplF"
                     /locus_tag="PXE44_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL5588343.1"
                     /translation="MSRVAKAPVNIPAGVEVKLNGQLLTVKGKNGELSREIHNAVEVK
                     QEADALTFAPREGIANADAQAGTARALVNNMVIGVTEGFTKKLQLVGVGYRAQMKGNA
                     VALSLGFSHPVEHTLPAGVTGECPSQTEIILKSADKQLIGQVAADIRAYRKPEPYKGK
                     GVRYSDEVVRTKEAKKK"
     gene            complement(10494..10886)
                     /gene="rpsH"
                     /locus_tag="PXE44_000814"
     CDS             complement(10494..10886)
                     /gene="rpsH"
                     /locus_tag="PXE44_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL5588344.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKVAISMPSSKLKVAIANVLAEEG
                     YIESVKVVEGVKPELEITLKYFQNKPVVESIQRVSRPGLRIYKRKDELPKVMGGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(10923..11228)
                     /gene="rpsN"
                     /locus_tag="PXE44_000815"
     CDS             complement(10923..11228)
                     /gene="rpsN"
                     /locus_tag="PXE44_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL5588345.1"
                     /translation="MAKQSMIARDVKRAKLADKFYAKREELKKIISDVNASDEDRWAA
                     VLKLQTLPRDSSPIRQRNRCRQTGRPHGVLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(11240..11779)
                     /gene="rplE"
                     /locus_tag="PXE44_000816"
     CDS             complement(11240..11779)
                     /gene="rplE"
                     /locus_tag="PXE44_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL5588346.1"
                     /translation="MAKLHDYYRDTVVNELKAKFNYSSVMQVPRIEKITLNMGVGEAL
                     TDKKLLDNAVADLAAISGQKPLVTKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLNAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTSA
                     KTDEEGQALLAAFNFPFRK"
     gene            complement(11797..12108)
                     /gene="rplX"
                     /locus_tag="PXE44_000817"
     CDS             complement(11797..12108)
                     /gene="rplX"
                     /locus_tag="PXE44_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL5588347.1"
                     /translation="MAAKIRQNDEVIVLTGKDKGKRGKVTKVLPNGKVFVEGINIITK
                     HEKPVPALGKAGGLVKKEAAIDVSNVAIFNPETNKADRVGFRFEDGKKVRFFKSNEKT
                     I"
     gene            complement(12119..12490)
                     /gene="rplN"
                     /locus_tag="PXE44_000818"
     CDS             complement(12119..12490)
                     /gene="rplN"
                     /locus_tag="PXE44_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL5588348.1"
                     /translation="MIQEQTMLDVADNSGARSVMCIKVLGGSHRRYAAIGDIIKVTVK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGAVIRFDGNACVILNNNTEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(12713..14548)
                     /gene="glmS"
                     /locus_tag="PXE44_000819"
     CDS             complement(12713..14548)
                     /gene="glmS"
                     /locus_tag="PXE44_000819"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL5588349.1"
                     /translation="MCGIVGAVAQRDIAEILVDGLHRLEYRGYDSAGVAVLNADDKTM
                     QIVRRVGKVKALDEALDTKPLLGGTGIAHTRWATHGEPSETNAHPHRSGKIAVVHNGI
                     IENYEELKIVLQERGYVFQSQTDTEVIAHLVEWELRSASSLLEAVQKTVVQLRGAYGT
                     VVLNEEEPEHLIVARSGSPLVIGYGVGENFLASDPLALLSVTRRFAYLEEGDVAEITR
                     RTVDIYDREGNKAEREIHEGNFEADAADKGQYRHYMQKEIFEQPVAIMNTLDGRIKDG
                     KVDISAIAPNAAEILSKVEHVQIVACGTSYNAGMVSRYWFESIAGVSCDVEIASEFRY
                     RKFVTRPNSLLITLSQSGETADTLAALRLAKESGYMAAMTVCNVASSSLVRESDFAFL
                     TKAGVEIGVASTKAFTTQLTCLLLLNVAIGRLQGRMSEEQEHHIVQSLQRLPAQIESA
                     LVFDKQIEKLSEDFADKHHTLFLGRGEYYPVAMESALKLKEISYIHAEAYAAGELKHG
                     PLALIDSDMPVVVVAPENDLLEKVKSNIEEVRARGGQLYVFADHDAGFEEAEGFKTIV
                     MPKVDEVTAPIFYTVPLQLLSYHIALIKGTDVDQPRNLAKAVTVE"
     gene            complement(14596..15357)
                     /locus_tag="PXE44_000820"
     CDS             complement(14596..15357)
                     /locus_tag="PXE44_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL5588350.1"
                     /translation="MSKRNTQQRRHLIVQMVQEKNEVSVDELAHFFETSEVTIRKDLT
                     ALEESGFLLRKYGGAVKIPSEMMEEAFIEQLSIQKKMIAQAARDCIRDHNRIIIDSGS
                     TTGALVKALNQTGLVVMTNSLNLATELTSLECEPTVLMTGGTWDSRSESFQGKVAEQV
                     LRSYDFDQLFIGADGLDLARGTTTFNELVGLSQVMAEVSREVIVLIESQKIGRKMPNI
                     ELKWQNITKIITDSQLSFETKEQIEKMGVEVVIAK"
     gene            complement(15514..15714)
                     /locus_tag="PXE44_000821"
     CDS             complement(15514..15714)
                     /locus_tag="PXE44_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL5588351.1"
                     /translation="MKSILKVVGAMVLMVAVSGCNNMTKTQRNTAIGAAIGGVAGHAI
                     GESTGATLGGAALGGVIGSQIK"
     gene            15939..16973
                     /locus_tag="PXE44_000822"
     CDS             15939..16973
                     /locus_tag="PXE44_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5588352.1"
                     /translation="MKLKKLSLALSTLLLGSAFANSALAEGRLTVYCSATNELCEQEV
                     QAFGKKYNVKVAFVRNGSGSTLAKIEAEKNNPQADVWYGGTLDPHSQAAEMGLLEPYK
                     SPNLEQIIEKFREPAKLKGNYSSAVYMGILGYGVNLDRIKKLGIEKVPSTWEDLLDPR
                     LAGEIQIADPQSSGTAYTAIATFVQLLGEEKAFDYFRKLHKNISQYTKSGITPARNTA
                     RGETAIGIGFLHDYAIEKKNGANIEMTAPTDGTGYELGGVSILKGARNLDNAKLFVDW
                     SLSKEAQELSWQKGNAFQVLTNTTAESSPYALDPKNLNLINYDFEKYGSSEERKRLID
                     KWVNEVKLAK"
     gene            17041..19077
                     /locus_tag="PXE44_000823"
     CDS             17041..19077
                     /locus_tag="PXE44_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016534092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5588353.1"
                     /translation="MQNFSFSRPLVWVLIGLLGFLLLPSKALDYGLFDSTSDELLAAM
                     GWASLNITWAWFLPLLVFLLPNSTACKTRAKLELVGLGVLFAFIFLSAIVLKISLGYA
                     VLPLIIAIMGLATPNLATLKVMQGDRFIIGSLLSIILLIFFFIVYPTIAIFVSMFYNG
                     SEFVPSQVLTILQQPYVVRVIGNSLSVAGTVGVLSTLFGLAFALYTTRIAKRSAFIGR
                     IFSILPIVTPPFVVGLGVTLMLGRSGYVTEFLVDYFGFANNWLYGFTGIVIAHTLALT
                     PMSFMILEGALKSIHPSVEEAAYTLRSNRYQAFFNIIFPLLKPALANSFLVVAIQSLA
                     DFSTPLVLGGSFDVIATQIYFYIAGSQLDYASASTLGAILLVFSLAIFVIQYLWIGNR
                     SYVTVSGKSYRGDVQDLPSGMKWLIICTLLFWGTFNLVLYGSIFYGSFTVNWGVDYTL
                     TLDNYIKLFGQGFSDGGFPSLIQTVIFAAAAAPITALFGLLIAYVTVRRDFKGKKTLE
                     FLTLLCFAVPGTVAGVSYILAFNDAPIYLTGTGMIIILSMVMRNMPVGMRAAIAGLGQ
                     LDKSLDEASLSLKAGSFKTMLFVVLPLLKPALLSALVTSFVRAMTTVSAIVFLVTADT
                     RVATSYILNRVEDGEYGIAIAYGSILIVVMMAIILIFDWIVGDTRIARSKAKKM"
     gene            19175..20224
                     /gene="fbpC"
                     /locus_tag="PXE44_000824"
     CDS             19175..20224
                     /gene="fbpC"
                     /locus_tag="PXE44_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDL5588354.1"
                     /translation="MNNDFLVLKNVTKSFGKVTVIDNLNLSIPQGKMVTLLGPSGCGK
                     TTVLRLVAGLENPTSGQIFIDGEDVTQSSIQHRDICIVFQSYALFPHMSIGDNVGYGL
                     KMQGVPKEERLQRVKEALELVDLGGFEDRFVDQISGGQQQRVALARALILKPKVLLFD
                     EPLSNLDANLRRSMREKIRELQQRLNITSLYVTHDQSEAFAVSDEVIVMNKGKIMQKA
                     PAKELYLRPNSLFLANFMGESSIFEGTIAGNQININGYQFTLANAEQFELPDGECLVG
                     IRPEAIHLAEQGNISQLCKIKNAVYMGNHWEIIAEWEGKDLLVNTKPENFYPHIKQAY
                     VHFIEQGVFLLKKEL"
BASE COUNT         5740 a         4553 c         4021 g         6017 t
ORIGIN      
        1 gcggtcgatt ctctgccaaa ttttgcaaat tttagaagaa aacagaccgc ttgtcagggt
       61 tggaattaga gtaaccagct gccaatcaat gcacaggcaa aaccggttaa accgatggca
      121 gtttcaagca ttgtccacgt tttgagggta gttttttcgt ccatttcaag gaaacggctc
      181 atcagccaga aaccggagtc attgacgtgg gaaagcaccg tcgcaccgga ggcgatagca
      241 atcacgataa aacagaggtc gaactggctt aaatcagtcg ctgccgccac agttggcgca
      301 atcaacgctg ctgtggtggt taaagcaacg gttgccgagc cttgtgcgac acggaatacc
      361 gtagcaataa taaaggcggc aaccacaatc ggcattccgg tgtctttcat catttccgat
      421 aatacatcgc caatcccgct ggcacgcaac acgccaccaa acattccgcc tgcaccggtt
      481 actaagacaa tcgagcagat tgggccaagg gcattgttac agattttttc gatttggtcg
      541 aaactgcgat tatcccgcaa cagcataatc gccacaatta aggtaatcag taaagcaaca
      601 ggggttttgc cgattaagcg aaggctctgc acccacattt cactgccatc aatcacttta
      661 gcaacggaga gggtattcaa gccggtatca aacaaaatca ggataatcgg cagcaccaac
      721 accgtgagta ccttgccaaa acttggcggg ttttgtactg ccatttcgtt tgccgataag
      781 ccgtttaaaa acgctttcgg caaatcaacg tgaatgcgtt tgctgataaa ttggctgaac
      841 aggtaagtgc cgatatacca cgttgggatt gcacaaacca agcccaccaa gactaacagc
      901 cccatattca caccaagcag atcgccggag gcaaccgggc cggggtgcgg aggtagaaag
      961 gcgtgcatta ccgcaaacgc accggcagac ggaaacgcat agcgcaacac cgaaccgccg
     1021 aactgttttg ccacgctaaa aataatcggt aacatcacca ccaagccggc atcaaagaaa
     1081 atcgggaagc cgaataacag cgaggcaacg cccaacgcaa aaggcgcacg ttctttgccg
     1141 aatttattga taagcgtatc cgcaagcact ttcgccccgc cggtgatttc aagcaagcga
     1201 ccaatcatcg ccccaagccc caccagtaac gccactgatg ccagcgtact accaaaacca
     1261 ttcaacaatg ttggcaggat tttatctacc ggaataccgg cagctaatgc agtgagcagg
     1321 ctcacaataa tcaatgccac aaaagcgtgg actttaaatt tcataatcag caccagcaat
     1381 aaaatgatgg cagcaaacat aatgaacagt aacataggat ctcctaaggt tgaattgtta
     1441 tcggtaacat aaccaaaaag cagtttagag taaacggttg cagggctttt attttaaaaa
     1501 tgtgatctac ttcaagtttt ctggaaatag cagatgattt ttcagcataa cctgttacat
     1561 taacggctaa gttaacgtta ccagtaacac aaggaaattc aatggcaaac ggcaaagcat
     1621 ttattttgat gggcgtgtcc agcaccggca aaaccagcgt gggaacggca gtcgcacagc
     1681 ggttgggaat aaaattgatt gatggggacg acttacaccc tcgtgccaat atactgaaaa
     1741 tggcaagtgg cacgccactc aatgatgcag atcgcacacc gtggttggag cggattaacg
     1801 atgcagtgtt cagttttgaa caaaaaagcg aaaaagggat tatcgtctgc tcagcattga
     1861 agaaaaagta ccgagaccag atccgacaag gcaatgccga tgtgactttt ctattcttgc
     1921 acggctcgtt tgaactggtg ctggctcgaa tgcaacaacg caaagggcat tttatgaaac
     1981 cggaaatgct tcaaagccaa tttaatacgc tggaaattcc gcaggcagac gaaccagatg
     2041 tgatatttat tgatattgac ggcagttttg acgaggtggt ggagcgttgc gtgaaagcag
     2101 ttgagccgtt gctttaatca caagtggtgg attttgcgga tttttttgca aatttaaccg
     2161 cttgtccggg ctacacactt tcccccaaat ggatctgata gcccaagtta attatggagc
     2221 tttgttgtgg tacgccgtga atacggttga gcagctcttg ggcggcaact ctgcctattt
     2281 caaggcgagg tgtgatgacg ctggcaagct gtggcgtgag ggattgcccg acatcgtgtc
     2341 cgtgaaagcc ggcgatttta atctgctgcg gcacatgaat gcctaaccgt tggcactcaa
     2401 ataatgcccc gattgccaaa tcgtcattgg tgcaaaaaat gccgtctagc tgcggaatgt
     2461 gttgcagggc ttgttttagc tggcttgcgc caagcgtgaa agaagaatgc tctgcagtag
     2521 taatgctgtg tgggcttaag ccgtgtttga gcattgcctg ttcgtagccc tgcattttca
     2581 gctgagagcg tttatccatt ctcgccccga aatacaccac ctgtcggcag cctcgatgaa
     2641 tcattgtctc gaccattgct tgagccgcag aaatattgtc aaatccgacc gcttgttact
     2701 actcttgcct gaactctttc ggggttttac caaactcttt tttaaacacg gcatagaaat
     2761 attgtaaaga gggataaccg cacacattgg caatttcttg ggtcgaaatc tcggtaaatt
     2821 tgagcatata tgttgctctt gctaattttt cctcgtgaat cacctggtga attgtcttat
     2881 tcatttcctg cttaaaacgc tgttcaaggt tagagcggga aattttaaga tgatccagta
     2941 cttgctctac tttaatgcct tggcaagctc ggtgacgaat aaagtgcata gcttggatta
     3001 ccattgggtc ttgcaaagaa cgataatcgg tcgaacggcg ttcttccact tttaccggcg
     3061 ggattaaaat cggcttggtt gaaaccggta gattatttaa ttttcgatat aataacttcg
     3121 cagcgtgata gccgatttgt gatgttcctt gtgtgactga agaaagagaa acccgagaga
     3181 ggtattgaat caactcttcg ttatcaatgc cgatcacgca aagttgttca ggtacagcga
     3241 tttttaaatg ctcacaggtt tgaagcaggt gtcttgccct tgcgtcagta acggcaataa
     3301 tgcccgtgtg ggctggtaag gtttgtaacc actcgcctaa tttggcttgt aattcaaccc
     3361 aattatctga atgaaattga tcaccaatat aacaatttgg gtgatgttca tatttttcca
     3421 tcaactgaat aaaggcattt tttcgctcta tcgcccaatg tttttccgtt tctgcaggat
     3481 agccgtaaaa cgcaaattgg ttgattcctt tttgttttag gtgtaaaaat gctgcctcaa
     3541 ctaaggcgta attgtcggta gcaacataag gaaggtcagg gtaatagtca gggttttggt
     3601 aagaaccgcc tactgctaca atggggacgt caatatcttt aagtagctca actgtttcca
     3661 aatcgtcaaa atcggcaata atgccgtcaa tcgacaggct ttcgatacta tttttatgat
     3721 agacaaaatc atcttccaca aaaatatccc aagtacattg tgatgcttga ataaactgcc
     3781 caatgccctg cattactcct ctgtcataaa ttttgttagc attaaaaagt aaggcaattt
     3841 tataagattg ttctatcatt acttcctcca gtacccactt tcaaaagtaa ctataaaata
     3901 aaattatttt ttgatcaaaa cgaataaaag aataaaaccc cggtatttct accagggttt
     3961 taagctcaat tttgattaag attattccgc agctgcttca gttgcaactt ctgggcgatc
     4021 cactaactca atgtaagcca ttggagcatt atcacctgca cggaaaccac attttaaaat
     4081 gcgagtgtaa ccacccgcac gttgtgcaaa acgtggacct aattcattga ataatttagc
     4141 aactgtatca acgttgcgtg tacgggcaaa agctaaacgg cgatttgcaa cgctgtctgc
     4201 tttagctaaa gtaattaacg gctcaactac acggcgtaac tctttcgctt ttggtaaagt
     4261 tgtcttgatg atttcgtgtt caacaagcgc acttgccata ttacggaaca tcgcttggcg
     4321 atggctgctg ttacggttta attgacgtcc actcttacga tggcgcataa cctattcctt
     4381 cttagaaaat ctataccgaa aaactagtct tctgcaatac ttgcaggtgg ccagttttca
     4441 aggcgcatac cgagtgataa accacgagaa gcaagaacat ccttaatctc tgtaagagat
     4501 tttttaccca agttcggggt tttaagaagt tcaacttctg tacgttgtac taaatcaccg
     4561 atatagtgaa ttgtctctgc tttcaaacag ttagcagaac gaactgtcag ctctaggtca
     4621 tctactggac gaagaagaat aggatcaaat tccggttttt cttctttcac ttcaggttga
     4681 cgaacatcac gcaaatcaac gaatgcagca agttgttctg ctaaaatcgt tgcagcacga
     4741 cggatagctt cttcaggatc taacgttccg ttagtttcca tctcaataac aagtttatct
     4801 aagtctgtac gttgttcaac acgagcagct tcgacattgt aagcaatgcg atctacaggt
     4861 gaaaaacgtg catctactaa taaacgaccg atagggcggt catcgttttg agtatgaaga
     4921 cgtgctgatg caggaacata acctcgacca cgttgaacac gaatgcgcat attaattgat
     4981 gcattcttat ctgttaaatg acagattacg tgatcaggat tcacaatttc tacatcgcca
     5041 tcgtgtgtga tgtcggctgc agttacaggg ccaattccag atttatttag tgtcaaaata
     5101 atatcatctt tattttgcac ttttaccgct agacctttaa ggtttaataa tacctcaaga
     5161 atatcttcct gaacgccttc tttgctgctg tactcatgca gtacaccatc aatttcaact
     5221 tcagtaactg cacaacccgg catagatgaa agtaaaatgc ggcgaagtgc gttacctaat
     5281 gtatggccaa aaccacgctc taacggctct aatgtcactt tagcatgagt tgaactaact
     5341 tgttcaatat ttactaagtg cggctttaaa aattctgtca cagaaccctg cattttttcc
     5401 tctctttgcg gttaagctaa ctattattta gagtaaagct caacgatcag atgttcatta
     5461 atatctgctg atagatcaga acgctctgga cgacgtttaa atacgccttc cattttagtt
     5521 gcatcaactt ctaaccaagt tggtttttca cgttgagttg ctaattctaa tgatgcttta
     5581 atacgtgctt gttttttcga tttttcacga acagcaacaa catcatcaac agaaacttgg
     5641 aatgaaggaa tgtttactac acgaccgttt actacgattg atttatggct aacaagctga
     5701 cgtgcttctg cacgtgttgc tgcaaaaccc atacgataaa ctacgttatc taaacgacct
     5761 tccaataata ctagtaagtt ttcaccggta ttacctttta agcggttcgc ttctgtataa
     5821 taattacgga attgacgctc taagatacca taaatacggc gaactttttg tttttcacgt
     5881 aattgactac catagtcaga taaacgtggc ttgcgagcac catgttgacc aggcgctaca
     5941 tcaagtctat ttctacattt tgattcaatc gcacgaacgc ctgattttaa gaataaatca
     6001 gtaccttcac gacggcttag cttgagctta ggacccaaat atcttgccat tttctttctc
     6061 caattctttt aacgtcaatt aaacacgacg ttttttcggt ggacgacaac cgttatgtgg
     6121 aatcggagtc acatcagtaa tattcgtgat acggaaacct gctgcattta atgcacggat
     6181 tgttgactca cgacctggac ccggtccttt aaccataact tccaaattct ttaaaccaaa
     6241 ctctttaacc atttctgcac aacgctctgc agcaacttga gcagcgaatg gagtcgattt
     6301 acgagaacca cggaaacctg aaccacctgc agttgcccaa gctagagcat taccttgacg
     6361 gtcagtaata gtaacgattg tgttattgaa agatgcgtgg atatgagcta cgccatctgc
     6421 aatctgtttt tttacgcgct tacgtgcgcg aactggtgtt ttagccatta tttatctact
     6481 ccgactattt tttgatcggt ttgcgtggac ctttacgggt acgcgcatta gtcttagtac
     6541 gttgaccacg taccggtaaa ctacgacgat gacgtaaacc acggtagcaa cctaagtcta
     6601 aaagacgttt gatgcttaaa gttacttcac gacgtaaatc cccttcgaca gtaaatttac
     6661 caacttcttc acgcagtttt tcaatctgct cttcagacaa ttctctgatc tttacatctt
     6721 cagcaatacc cgttgcagca cagatggctt ttgcacgagt tttaccgata ccgtaaattg
     6781 cagttaatgc gattacagtg tgtttttgat caggaatgtt aatgcctgca atacgggcca
     6841 ctatgcactc ctattattta agtttattga tatactgatc ttgaaaagcc cgtttcagga
     6901 tactcaaaca gtatatcagg acgaatgagc tgagcagtat aactgctcag cgggttcttt
     6961 gcaagaaata tgcgtaaatt aaccttgacg ttgtttgtgt ttaggatcgc tacaaattac
     7021 acgaaccaca ccttcacgtt tgattacttt acaattacgg cacattcttt ttactgaagc
     7081 acgaactttc attgcttatc ctttattaac ggcctattaa tttagacctt ttaagttagc
     7141 tttcttcaac acagaatcat attgttgaga cattaaatga ctctgaacct gtgcgatgaa
     7201 atccataata acaaccacta caatcaataa tgatgtacca cctaattgga atggcacctt
     7261 ccataacgac gtaatgatgt aaggaaccaa acaaacaaaa gcaatatata acgcaccaat
     7321 taaggttaaa cgtgtcatta ctttatcgat ataacgagat gtttgttcgc ctggtcggta
     7381 acctggtaca aacgcacctg atttcttaag attatccgct gtatcacgag gattatactg
     7441 cattcctgta taaaagaaag caaagaaaat cactgccatt gtgaataata caacatacag
     7501 tggttgacct ggctgtaata gttgtgaaag gttaaataac cactcaaatc cttcgctttg
     7561 accaaaccat tgagtaatgc ttgccgggaa taaaataata cttgaagcaa aaattgccgg
     7621 tattacccct gccatattca cttttaatgg taagtgagaa gttcttgcag gcgccatcat
     7681 tcgaccttgt tggcgacttg catagtttac aactattctt ctttgtccac gttcaacaaa
     7741 aacaacaaaa taagttactg caaatgcaat tattgcaact agtagtaaga cgataaatga
     7801 taactcgcct tgacgggctt gttctattgt ctgccctatc gttgctggaa gatctgcaac
     7861 aatacctgta aaaataatca atgagatacc gtttccgata ccacgctcag tgatttgttc
     7921 gcctagccac attaggaaca ttgttcctgt cactaagctt actacagagg ttacatagaa
     7981 taatatactt ggattaggaa ctaatccatg taccatattc ggtagggcta gtgaaatacc
     8041 aatagattga ataaatgcta ataacagtgt gccatagcgt gtatatttac tgattttccg
     8101 acgacctgct tcaccttctt tctttaattc agctagaggt gggtgaatcg ctgtcaataa
     8161 ttggataata attgacgctg aaatataagg cataatacct agtgcaaata tagatgcacg
     8221 gcttaaagca ccaccagaga acatattaaa catatcaatg atggtgcctt gctgttgtcg
     8281 aattaattcg gataatacag aagcatcaat gccaggaaca ggtataaaag aaccgatacg
     8341 gaaaacgatt aacgcaccta aaacaaacaa tagtcttgat ttaagctcgc cagtcccttt
     8401 ttgtgtacta ccttggtacc ctggttgctt tgccatcttt attcctcgat agaaccgcca
     8461 gcagcttcaa tagcagcttt agcgccttta gttacacgta agcctttaac aactactgct
     8521 ctttcaattt tgcctgctaa aattacttta gcagataaaa tatctttagt aatgatgttt
     8581 gctgctttta atgtctctaa agttacttcg ttgccatcta ctttagctaa gtcgtttaaa
     8641 cgaatttcag ccacatggaa tgattttaat gaagtaaaac caaattttgg caaacgacga
     8701 tataaaggca tttgaccacc ctcgaaacca cgacgaacac cgccgcctgt acgagatttt
     8761 tgacctttat gaccgcgccc accagtttta cctaaaccag aaccaatacc acgacctaga
     8821 cgttttgcac tgtgcttagc accttcagct ggagaaagag aatttaaacg cattctatta
     8881 ctcctctact ttaaccatat aagacacttg attgatcata ccacgcactg ctggtgtatc
     8941 ttcaagttct acagtatgac gaatatgacg aagacctaag ccttttaacg ttgctttatg
     9001 tttcggtaaa cgagcgatag agctacgagt ttgagttact ttaatttttg ccatatccaa
     9061 ttaccccaaa atttcttcaa ctgttttgcc acgttttgca gcaaccattt caggtgattt
     9121 catgttctca agtgcatcga ttgttgcacg aacaacgtta attgggttag ttgaaccata
     9181 cgctttagaa agaacgttac gaacacccgc aacttctaat actgcacgca ttgcaccacc
     9241 agcgatgata cctgtacctt cacttgctgg ttgcataaat acgcgtgaac ctgtatgtga
     9301 acctttaact ggatgttgta atgtaccttc atttaaagct acattaatca tattgcgacg
     9361 agctttttcc atcgcttttt ggattgctgc tggaacttca cgtgctttac cgtgaccaaa
     9421 acctacacga ccgttaccat cgcccactac tgttaaagca gtgaaactca taatacgacc
     9481 accttttacg gtttttgaaa cacggttaac cgcgattagc ttttcctgca gttcaccagc
     9541 ttgtttttcg atgtttgaca tctcaaattc ctctattaga actgtagacc agcttcacga
     9601 gcagcatctg ctaatgattg cacacgaccg tggtatttaa aaccagaacg gtcaaaagca
     9661 accgctttaa tacctttggc aattgcacgc tcagcaacga ttttacccac tactgctgcg
     9721 gcttctttat tgccggtata tttaacttgc tctttaatta ctttttcaac agttgaagct
     9781 gctgctagta cttctgagcc attaggtgca atcacctgcg catagatatg gcgaggtgtg
     9841 cgatgcacaa ctaaacgtgt tgcaccttgc tctctcatta aatgacgtgc acggcttgca
     9901 cgacggatac gagctacttt cttatccata gtgttacctt actttacttt ttcttcgctt
     9961 cttttgtacg cactacttca tcagagtaac gtacaccttt acctttataa ggttcaggtt
    10021 tgcgatatgc acgaatatct gctgccactt gaccaattaa ctgtttgtca gcacttttca
    10081 gaataatttc tgtttgtgat ggacattcac cagttacgcc tgctggcaac gtgtgttcta
    10141 ctgggtgaga gaaacctaaa cttaaagcaa cagcattgcc tttcatttgt gctctataac
    10201 caacacccac taattgcaat ttcttagtaa agccttcagt aaccccgatt accatattat
    10261 ttactagtgc acgagcagta cctgcttgag catctgcatt agcgatacct tcacgtggag
    10321 cgaaagttaa tgcatctgct tcttgtttta cttcaactgc attgtgaatt tcacgagata
    10381 actcgccgtt tttaccttta actgttaata gctgaccgtt aagttttacc tcaacaccgg
    10441 caggaatatt aacaggtgct tttgcaacac gagacatttt tcctacctct ctattatgct
    10501 acgtaacaga taatttcacc gccgaggccc gcttgacgcg cagcacggtc ggtcattaca
    10561 cctttagatg tagaaacaac tgcgatacct aaaccaccca ttacttttgg taattcgtct
    10621 ttgcgtttat aaatacgcag acccggacgg ctcacacgtt ggatactttc tacaaccggt
    10681 ttgttttgga aatattttaa agtaatttcc agttcaggtt taacaccttc aacaacttta
    10741 acgctttcga tataaccttc ttcagctaaa acatttgcaa tagcaacttt taacttagat
    10801 gaaggcatac tgattgcaac tttgttcgca gcttgaccgt tacgaatacg ggtcagcata
    10861 tctgcgattg gatcttgcat gctcatgtat ttactcccat gattccaaat taaaagaggt
    10921 tattaccagc ttgccttctt aagacccgga atttcaccgc gcatagcagc ttcacgaacc
    10981 ttaatacggc ttaaaccaaa cttacgaagt acgccatgtg gacgaccagt ttggcggcaa
    11041 cgattacgct gacggattgg gctagaatca cgaggaagtg tttgtaattt taacactgct
    11101 gcccaacggt cttcatcaga tgcattcaca tcagagatga ttttctttaa ttcttcacgt
    11161 tttgcgtaga atttatcagc taatttagca cgttttacat cgcgtgcgat cattgattgt
    11221 tttgccacga taacctacct tatttacgga atgggaaatt gaaagcagct aataaagctt
    11281 gaccttcttc atcagttttc gctgaggtgg tgatagtaat atctaaacca cgaacacgat
    11341 ctactttatc gtagtcgatt tcagggaaaa tgatttgttc acgaacaccc atactgtaat
    11401 taccacgacc atcgaatgac ttagcgttta aaccgcggaa gtcacgaata cgtggaacag
    11461 cgatagtaat caatctttca aagaattccc acatacgttc accacgtagg gttactttgc
    11521 aaccgattgg atacccttga cggattttaa agcctgcaac agatttgcga gctttagtta
    11581 ctaaaggttt ttgaccgctg attgctgcta aatccgctac tgcgttatct aacagttttt
    11641 tgtcggtcaa tgcttcaccc acacccatgt taagggtaat cttttcgatt cgtgggactt
    11701 gcatgacaga tgagtagttg aattttgctt ttaattcatt aactactgta tctctgtagt
    11761 aatcatgcag tttcgccatc acattactcc agttaattaa attgttttct cgttagattt
    11821 gaagaaacgc acttttttgc catcttcgaa tctaaatcct acacggtcag ctttgttggt
    11881 ttcagggttg aagatcgcaa cgtttgaaac atcgattgca gcttctttct tcactaaacc
    11941 accagctttg cctaatgcag gaactggttt ttcgtgttta gtgatgatgt tgataccctc
    12001 aacaaaaact ttaccatttg gtaacacttt agttacctta ccacgtttgc ccttatcttt
    12061 accggtaaga acaattactt catcgttttg acggattttt gcagccatta cattctcctt
    12121 atagtacttc tggagctaaa gagatgattt tcataaactt ctcagaacga agttcacgag
    12181 tcaccggtcc aaaaatacga gtaccgattg gttgctcagt gttattgttt aaaattacac
    12241 aagcattgcc atcgaaacga ataactgcgc catctgggcg acgaacaccc ttcttggtgc
    12301 gcacaacaac tgcttttaac acatcacctt ttttcacttt accgcgtgga attgcttctt
    12361 ttacagtaac tttaatgata tcgccaatag cagcgtaacg acggtgcgat ccacctagaa
    12421 ccttgataca cattacgcta cgagcccctg agttatcagc aacatccagc atagtctgtt
    12481 cttggatcat attagtgctc cgttaaaata aaaaaacacc ctttcgggac gaacctattt
    12541 atacaaatat aaatagggtg caaaatatac tacaaaaacc actagttaat caagtgaatt
    12601 tttaagctaa tttaatagtt taagtaagct tatttataaa ttgtttcaac tatcaataaa
    12661 ctgtcctata actcatataa aaaccccaag atatcttggg gtttaactaa cattactcta
    12721 cagtcaccgc ttttgcaagg ttacgaggtt ggtcaacgtc cgttcctttg attaaggcaa
    12781 tgtgatatga aagtagttgt aacggcacgg tgtagaaaat cggtgcggtt acttcatcaa
    12841 ctttcggcat tacaatcgtt ttgaagcctt ctgcttcttc aaaacctgca tcgtgatcgg
    12901 caaacacata taactgaccg cctcttgcac gcacttcttc aatattggat ttcactttct
    12961 caagcaagtc attttccggt gctaccacca ctaccggcat atcgctatca attaatgcta
    13021 acgggccgtg ttttaactcg cctgcggcat aagcttctgc gtgaatatat gaaatctctt
    13081 ttaatttcaa tgcagattcc atcgcaaccg ggtaatattc tccacgacct aagaacaatg
    13141 tgtggtgttt atccgcaaaa tcttccgata atttttcgat ttgtttatcg aacactaacg
    13201 cactttcaat ttgtgctggt aagcgttgta aagattgcac gatatggtgt tcttgctctt
    13261 cactcattct gccttgtaag cgtccaatcg ccacatttaa taacaataag caagttaatt
    13321 gagtagtgaa tgctttggtt gatgctacac caatttcaac accagctttg gttaagaatg
    13381 caaaatcgga ttcacgcact aatgaagaac ttgccacatt acacaccgtc attgccgcca
    13441 tatagccaga ttctttcgct aaacgcagtg cagctaacgt atctgcggtt tcacctgatt
    13501 gtgaaagcgt gattaataag ctgttcgggc gagtaacgaa tttacggtaa cggaattcgg
    13561 aggcaatttc cacatcacag ctcacgcctg caatggattc aaaccaataa cgtgaaacca
    13621 taccagcatt gtaagaggtt ccacaagcca caatttgcac gtgttctact ttcgataaaa
    13681 tctctgccgc attcggtgca atggctgaaa tatctacttt accgtctttg atacgaccat
    13741 ctaaggtatt cataatcgca accggctgct caaaaatctc tttttgcata tagtgacgat
    13801 attgtccttt atctgccgcg tctgcctcaa aattgccttc gtgaatttca cgctctgcct
    13861 tgttgccttc acggtcataa atatccactg tgcgacgggt aatttcggct acatcacctt
    13921 cttctagata agcaaaacgg cgagttacgc ttaataatgc aagtgggtct gaagcaagga
    13981 agttctcgcc tacaccgtaa ccaatcacta acgggctacc tgagcgagcc acaattaaat
    14041 gctccggctc ttcttcattt aataccactg tgccgtaagc accacgcagt tgcaccacgg
    14101 ttttttgtac cgcttccaat aatgaagaag cagaacgcaa ttcccattca actaaatgag
    14161 caatgacttc ggtatcggtt tgagattgga acacataacc acgctcttgt aacactattt
    14221 ttaactcttc gtagttttcg ataatcccgt tatgaactac tgcaattttg ccactgcggt
    14281 gtgggtgagc attagtttct gacggctcgc cgtgcgttgc ccagcgagtg tgtgcgatcc
    14341 cagtgccgcc taatagtggc ttagtgtcta acgcctcatc taaggctttg actttaccga
    14401 cacggcgtac aatttgcatt gttttatcat ctgcatttaa tactgctaca ccagcagagt
    14461 catatccacg gtattctaag cgatgtaaac catcaaccaa gatttctgcg atatcacgtt
    14521 gtgctactgc acctacaatt ccacacataa atttgctttc ctatgtttaa gtttaaaaat
    14581 tcatttgagc tactgctatt ttgcaataac cacttcgaca cccatttttt cgatttgttc
    14641 tttcgtttca aaacttaatt gactgtcggt aataattttg gtaatatttt gccatttcaa
    14701 ttcaatattc ggcattttcc gaccgatttt ttgcgattca attaacacaa tcacttcacg
    14761 gctcacttct gccatcacct gacttaagcc aactaattca ttaaaggtag tcgtgcctcg
    14821 agccaaatct aaaccgtccg caccaataaa aagctgatcg aaatcatatg aacgcaatac
    14881 ctgttctgct actttgcctt gaaaggactc agaacggcta tcccaagtgc cgccggtcat
    14941 caacactgtt ggttcgcatt cgagggaagt caattccgtc gctaaattca gcgaatttgt
    15001 catcaccacc aagccggttt ggttcaatgc ttttaccaaa gctccggtcg tgctgccgct
    15061 gtcaataatg atgcgattat gatcacgaat acaatcacgt gcggcttgag caatcatttt
    15121 cttttgaatc gaaagttgct caataaacgc ttcttccatc atttccgatg gaatttttac
    15181 cgccccacca tacttacgca ataaaaaacc actttcttcc aaagcggtta aatcttttct
    15241 aatcgtaact tcagacgtct caaagaaatg agcgagttca tctacactca cttcattttt
    15301 ctcttgtacc atttgaacaa ttaaatggcg gcgttgttgc gtgttacgtt ttgacattat
    15361 aatcttcact taaatttcgt ttcaaaactt ttgcattata aagaaagctc gcgaaaagaa
    15421 aagcagaaaa tgcaaaattt acaaaatcta tacaaaataa aaccgcttgt aatattgatt
    15481 tacaagcggt tgatttcttg aaattttttg caattatttg atttggctac caatcacgcc
    15541 accaagtgcg gcaccaccta aagttgcacc ggtagattca ccaatcgcgt gaccagccac
    15601 accgccaatt gctgcaccga ttgcagtatt acgttgcgtt tttgtcatat tgttacagcc
    15661 agacacagca accattaata ccattgcacc aacaactttc aaaatagatt tcataataat
    15721 ccctcataat taatcaattt caaataagcc aatattcttg gctatgctat aagatttaat
    15781 ttatttagaa aggttcaact actaccacct atgaattgca aattttttca caatttaggc
    15841 cgcttgttga ttgaactgtg agcttgctca cagaatttta tggtatttct tgctatattg
    15901 tatagcaatc tatgcgataa ctcattaagg aaataaaaat gaaattaaaa aaattatctt
    15961 tagcactctc gactttatta ctcggttcag cctttgccaa ctctgcttta gcggaaggtc
    16021 gtttaaccgt ttattgtagt gccactaatg aactttgtga gcaagaagtg caagcattcg
    16081 gcaaaaaata taatgttaaa gtagcatttg tgcgtaatgg ttcaggcagt acattggcga
    16141 aaattgaagc ggagaaaaac aatcctcaag ctgatgtatg gtatggcggt actttagatc
    16201 cacattcaca agctgcagaa atgggtttac tcgagccgta taaatcaccg aatctcgaac
    16261 aaattatcga aaaattccgt gagccggcaa aattaaaagg caactactct tcggctgtgt
    16321 atatggggat tttaggttat ggtgtgaatt tagaccgaat caaaaaactc ggcattgaaa
    16381 aagtgcctag cacttgggaa gacttgctcg acccacgcct agccggtgaa atccaaattg
    16441 ccgacccaca aagctccggc acggcttata ctgcgattgc gaccttcgta caacttttag
    16501 gagaagaaaa agccttcgac tatttccgca aattacacaa aaacatttca caatatacca
    16561 aatccggcat cacaccggca cgcaataccg cccgtggtga aaccgctatc ggtatcggtt
    16621 tcttacacga ctatgcgatt gagaagaaaa acggggctaa cattgaaatg accgcaccaa
    16681 cagacggcac aggctacgaa ttaggtggtg tgagtatcct caaaggtgca agaaatcttg
    16741 ataatgcaaa actgttcgtg gattggtcgc tttctaaaga agctcaagaa ctctcttggc
    16801 aaaaaggcaa tgcgttccaa gtattaacca acaccaccgc agaatcttcc ccttatgctc
    16861 tagatccgaa aaacttaaac ttaatcaact atgatttcga gaaatacggc tcgagcgaag
    16921 agcgtaaacg cttgattgat aagtgggtaa atgaagtaaa attagcgaaa tagtgttaat
    16981 ctttccactg aggggataag caattatccc ctctctaatt ataagaatct gtgaggtgtt
    17041 atgcagaatt tttccttttc ccgaccgctt gtttgggtgc ttatcgggtt actcggtttt
    17101 ctcttgttac cgtctaaagc cttagattat gggctgtttg actcaacctc tgatgaactg
    17161 ctcgccgcaa tgggctgggc gagtctgaac atcacttggg cgtggttttt accgcttttg
    17221 gtatttttat tgccaaattc gaccgcttgt aaaactagag ccaagctgga attagtaggg
    17281 ctgggcgtgc tgttcgcctt tattttcctg tcagcgattg tgctgaaaat cagcctcggt
    17341 tatgcggtgt tgccgttgat tattgccatt atggggcttg cgaccccaaa tttagcaacg
    17401 ctcaaagtaa tgcagggtga ccgctttatt atcggctcgt tactgagcat tattctgctg
    17461 attttctttt ttattgtgta tccgaccatc gccatttttg tgtcgatgtt ctacaacggc
    17521 tcggaatttg tgccaagcca agtgctgaca attttgcaac aaccttatgt cgttcgagtc
    17581 atcggtaact cgctctcggt tgccggcacg gtgggcgttc tctccaccct attcggctta
    17641 gcctttgctc tctacaccac tcggattgct aaacgctcgg cgtttatcgg gcggattttc
    17701 tcgattttac ctatcgtcac cccgccgttt gtggtcggtt taggcgtaac cctgatgctt
    17761 gggcgttccg gctatgttac tgaattttta gtggattact ttggctttgc caacaattgg
    17821 ctgtacggtt tcaccggtat tgtgatagcc cataccctcg cgctcacgcc aatgtcgttt
    17881 atgattttgg aaggggcatt gaaatccatt cacccatcgg tggaagaagc agcttacacc
    17941 ctgcgttcca accgctatca agcctttttt aacattattt tcccattgtt aaaaccggca
    18001 ttggcaaact cctttttagt ggtggcaatt caatccctcg ccgatttcag tacgccgtta
    18061 gtattgggcg gtagctttga tgtgattgca acccaaatct acttctacat tgccggttct
    18121 caactggatt atgcctctgc tagcacgctt ggggcaattt tgttggtgtt ctccttagcc
    18181 attttcgtga tccaatacct ctggatcggc aatcgctctt atgtgacggt gtccggcaaa
    18241 tcctatcggg gtgatgtcca agacttacca agcggtatga aatggctgat tatttgcacc
    18301 ttattgttct ggggaacctt caacttagtg ctgtacggca gtattttcta cggcagtttc
    18361 accgtgaact ggggtgtgga ttatacctta acccttgata actacatcaa gctattcggg
    18421 caagggttta gcgatggcgg tttcccttcg ttaattcaaa ccgtgatttt cgctgccgct
    18481 gccgccccga ttactgcact attcggcttg ctgatcgcct atgtgacagt tcggcgtgat
    18541 ttcaaaggca agaaaaccct ggaattttta accctgctct gctttgccgt accgggaacg
    18601 gtggcaggtg tttcttacat tctcgccttt aacgatgccc cgatttacct aaccggcacc
    18661 ggtatgatca tcattctctc aatggtaatg cggaatatgc ctgtgggtat gcgagcggca
    18721 attgccggct tagggcagtt ggataaatcg ctggacgaag cctcgctctc gctgaaagcc
    18781 ggttcgttca aaacgatgct gtttgtggtg ttaccgctac tcaaaccggc actgctctcc
    18841 gccctcgtca ccagctttgt gcgggcaatg acgactgtca gtgcgattgt attcttagtc
    18901 accgccgaca ctcgtgtggc aacctcctac attctcaacc gtgtggaaga cggtgaatac
    18961 ggcattgcga ttgcctacgg ctcaatttta attgtggtga tgatggcgat tatcctgatt
    19021 tttgactgga ttgtcggcga cacccgtatc gcccgttcta aggcgaaaaa aatgtaaaaa
    19081 gacatctact ctctcccttt atgggagaga gacagaaaaa tacttcgttc agaagtattt
    19141 ttcagagaga gggatataat taaaaggaca atatatgaac aacgatttct tagtattaaa
    19201 aaacgtcacc aaatctttcg gcaaggtgac ggtcattgat aatctcaacc tcagtattcc
    19261 gcaaggtaaa atggtgacat tactcgggcc gtccggctgt ggtaaaacca ccgtactgcg
    19321 tttagtggca gggctggaaa acccgacttc ggggcaaatt tttattgatg gcgaagatgt
    19381 tacccaaagt tcgatccaac accgtgatat ttgcattgtg ttccaatctt atgcgttatt
    19441 tccgcatatg tcgattggcg ataatgtcgg ctacggcttg aaaatgcagg gcgtaccgaa
    19501 agaagaacgg ctgcaacgag tgaaagaggc gttggaattg gtggatttag gcggctttga
    19561 agatcgcttt gtcgatcaaa tctctggcgg acaacaacaa cgtgtcgccc tcgcccgtgc
    19621 cttaattctg aaaccgaaag tgctactgtt cgatgaaccg ctgagtaacc ttgacgctaa
    19681 cttacgccgt tcaatgcggg agaaaatccg tgaattacag caacgtttaa acattacttc
    19741 cctttatgtg actcacgatc agtccgaagc ctttgcggtt tcagatgaag taattgtgat
    19801 gaacaaggga aaaattatgc aaaaagctcc agccaaagag ttataccttc gccctaattc
    19861 tttattctta gcaaatttta tgggtgaaag cagcattttt gaaggcacga tagctggcaa
    19921 ccaaattaat ataaatggat atcaatttac gttagcaaat gctgaacaat ttgagttacc
    19981 cgatggggaa tgcttagttg gcattcgtcc agaagctatt catttagctg aacaaggaaa
    20041 tataagtcaa ctctgtaaaa ttaaaaatgc agtttatatg ggaaatcatt gggaaattat
    20101 agctgaatgg gaagggaaag atttattagt caatactaaa cctgaaaatt tttaccctca
    20161 tatcaaacag gcctatgtac attttatcga acaaggtgtt tttcttttaa agaaagagct
    20221 ataacgaatt tatactaaat taagaggaag gtagttataa ctatttagca attttgagaa
    20281 aaataggccg catgcaaaat ctcttgatga caagcggtct tttctcgccg a
//
LOCUS       DANLZC010000028        20267 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.028, whole genome shotgun sequence.
ACCESSION   DANLZC010000028 DANLZC010000000
VERSION     DANLZC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20267
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.028"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            30..503
                     /gene="nrdG"
                     /locus_tag="PXE44_000825"
     CDS             30..503
                     /gene="nrdG"
                     /locus_tag="PXE44_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL5588355.1"
                     /translation="MENLRFSSEQIVWQEVPNETSLAFLITGCPLGCKGCHSADSWKA
                     CLGKELSSEYLSERLKRYQGLISCVLFMGGEWEAEKLLPLLQLAKKQGLNTCLYSGLE
                     QHEIAETLLHELTYLKTGRWIAERGGLESLTTNQRFTDLRNNQNLNHLFIRGASI"
     gene            500..2281
                     /gene="nrdD"
                     /locus_tag="PXE44_000826"
     CDS             500..2281
                     /gene="nrdD"
                     /locus_tag="PXE44_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL5588356.1"
                     /translation="MIRLQPTQLDGKLHFINQYIQAKNAADGSKMDANANVTQKNIAT
                     MEQELMKDFFVQINRAKVSEKIAKIFGKETACEYLRQIEAHEIYVHDETSLKPYCVSV
                     TMYPFLRDGLTKLGGESKAPKHLASFCGSFINFVFAVSSQFAGAVATVEFLTYFDYFA
                     RKDYGDNYLQTHRSDIENHLQQVVYSINQPAAARGYQSVFWNISVYDKFYFEAMFSRF
                     VFPDFEKPNWETTSKLQLFFMQWFNKERSKAILTFPVVTAAMLTEKGKCKDDEFANQM
                     AKELSQGNSFFIYQSDNPDSLASCCRLRNEISDHSFSYSLGAGGVATGSINVITINMN
                     RLVQDNRDLSEEVSKIHKYQYAYRKLMEEYLAAGMLPVYDAGFISLHKQFLTIGINGM
                     AEAAESQGLTVGYNPDYIDFVQSRLKIIFQANQAAGEKYGVKFNTEFVPAENLGVKNA
                     KWDKEDGYFVPRDCYNSYFYVVEDEEINALDKFLLHGRELIEWLDGGSALHLNLDEAL
                     TQTGYRSLLDTAAKTGCNYFCINVKITICNECSHIDKRTLHSCSKCGSDNIDYGTRVI
                     GYLKRVSAFSSARQKEHSLRFYHREAA"
     regulatory      2338..2434
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2491..3309
                     /gene="thiM"
                     /locus_tag="PXE44_000827"
     CDS             2491..3309
                     /gene="thiM"
                     /locus_tag="PXE44_000827"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDL5588357.1"
                     /translation="MTTSLPYHSHYLQKIRQNRPLVHNITNIVAANFSANGLLALGAS
                     PIMAAAIEEMESVPALSNAVVINIGTLIGKEVEAMELAGKTANRLGIPLILDPVGVGA
                     TPFRRQTVETLVDQIHFTAIRGNAGELATLAGVEWQAKGVDAGEGSHNVVEIAQQIAK
                     RYQTLVAISGETDVVSSGSHTALLHNGSPMFPKITASGCLLSAVCGAFLAVADKEHYF
                     NALIEACAAYAVAGEVAAQALKPTQYGQFAVNLLDTLGALTPETVEQYVRVEYV"
     gene            3302..4615
                     /locus_tag="PXE44_000828"
     CDS             3302..4615
                     /locus_tag="PXE44_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5588358.1"
                     /translation="MSKTHSNPTKVAVASMVGTAIEYFDNYIYTMAAVLVFNVQFFNS
                     DDPVSNQLMSLSVLALAFFTRPMGSILFGHFGDKYGRKQTLIASLLLMGLSTVAIGLL
                     PNYAAIGIWATVLLCLCRIGQGLGLGGEWGGAALVATEHAPKHKRAFFGIFPQMGAPI
                     GLLLANGAFYLVNLIYGEQTFLDWAWRIPFVASIVMIAIGLYIRIKISESPIFLKAEQ
                     QGKNRHTPLKTLFSQHLKPFMIGVLIATAGYVLFYILVAFTPIFVKSPVAVSQAGHAT
                     GLGLPVNLYTQYLLITSVIFGIAIFFSGIYSDKIGRRLLLLLATGATVVYGLTMPFFL
                     ENGTPTSIFIFLTIGMILIGLSFGPMAVILPELFPTEVRYTGASLAYTAAGILGASVF
                     TIIAVKINENFGLFGVGGYLSVASLLSFWALWQVHETKDLELEEI"
     regulatory      4751..4847
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4915..6444
                     /gene="thiE"
                     /locus_tag="PXE44_000829"
     CDS             4915..6444
                     /gene="thiE"
                     /locus_tag="PXE44_000829"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDL5588359.1"
                     /translation="MSNYSNSPYQPNVIWTVAGSDSCAGAGLQTDLHTFHDFNLVGCS
                     VVTSVTAQHPHGVLCVTPVDDHTFRQQFEALLVQGYPNAIKIGLLCSQAQVEILCEYI
                     QKIRAESSHYCYVVYDPVAVASSGQALSDSILLQIVQQKLYPLVDLITPNGTELALLS
                     DTEIQTFGDVKTASHKLFAQGIKAVLAKGGHFEWLGETVRDYLLTANEIYAFSHARQQ
                     SVNTHGTGCTYASAVGAMLATGFDLADAVTVATAYLQKGLSEKQGRGQTALSSLCHTG
                     YPDDIAFFPQTELISAPLKLPKEPFAPTEYQLGLYPVVDSVKWLERLILVGVKTLQIR
                     IKNRPLAEIEQEIAHSVALAKQHNVRLFVNDYWQLAMKYQAYGVHLGQEDLATADLNA
                     IQQSGLRLGVSTHCYFEIMRALAVKPSYIAFGHVFPTQTKVMPSQPQGLTNLAKYVAL
                     VAPLNIPTVAIGGINEQSIENVMHTGVGSAAVVSAVTQAKDWQQAVKNLAKFANGGRN
                     E"
     gene            6437..7609
                     /gene="cytX"
                     /locus_tag="PXE44_000830"
     CDS             6437..7609
                     /gene="cytX"
                     /locus_tag="PXE44_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HDL5588360.1"
                     /translation="MNRTLSTLNSGLIWAGAGISASAIVVGTWIAPLGWQQGLLAIVL
                     GHLLGGILFFAAGLIGAKTGKNAMQTVQISFGRGSVFFSLANVLQLVGWTAIMIYTGA
                     EISNALSLALWEYSAFSLWAIVLGLLIVLWLFTGSNQVGKFKLVTLIAMFLLTLWLSI
                     KVLNGQENFPVSGEMPFSTAVELATIMPLSWLPLVSDYTAKAKKPFAATLSATIGYLI
                     TSAWMYALGLGMVLFTGQTEIAPMLLMTGMSLIGIMVMILSTVTTTFLDAYSAGVSAG
                     NITAKFKETPTAILVTIIGTLLAISLPVTQYENFLLLIGSVFAPMIAVQIADFFVLKR
                     HQAQPNVDWVAAGLWLVGFVLYHLLSYHQVHLLLGLTIPVMLVIFLSTIFVRRFTK"
     gene            7648..7857
                     /locus_tag="PXE44_000831"
     CDS             7648..7857
                     /locus_tag="PXE44_000831"
                     /inference="COORDINATES: protein motif:HMM:NF015737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5588361.1"
                     /translation="MESLVLNNDWVVTPQAQLAYHTINGKADEERLNLLVARAGVRVA
                     KGFALNNGWNLQPYAEVNGIAEKGQ"
     gene            7865..8041
                     /locus_tag="PXE44_000832"
     CDS             7865..8041
                     /locus_tag="PXE44_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5588362.1"
                     /translation="MQVNQYQFGVAENKGRVQGSLGLSAGNGNHRLGLEASVASGKKL
                     KQPFNVLVNYRYQW"
     gene            complement(8082..9938)
                     /gene="sppA"
                     /locus_tag="PXE44_000833"
     CDS             complement(8082..9938)
                     /gene="sppA"
                     /locus_tag="PXE44_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL5588363.1"
                     /translation="MLTILKAFYRIFRCIRECVINLFFILFILLLVPVVAFISSSGST
                     QKPVFNQGALRLNLDGYLADNREEFTDFYRLVQSELGSSEPFKISTFDVVQAISHAKN
                     DPKITGLVLDLQKLQSADFSSLDFIGNEINNFKQSGKPVIAVGEDYSQKQYYLASFAD
                     EIYLNKVGAVDLKGLNYSNTYFKTLLDKIEAEPHIFRVGTYKSAVEPFMRDDMSEEAK
                     QNARGWLNGTWQQTVATLAQNRNIEPQALDLSPANYIEKYKQAKGDDAQLALNQKWVS
                     QLVSSQESRDKLIAQFGKNSEGSYNQIEFLDYMTELNDRFAEVNAPKIAVVNVEGAIA
                     GGESDEMNVGSETLVKLLQQARDDKNVEGLILRINSPGGSAVASELIRQEVEAFQQAG
                     KPVVASMGGMAASGGYWIAATSDKIIASPNTLTGSIGIFGLAVTFEKTAKNLGVSEDG
                     IATSALAQQAGLKSLPQEQAEVLQIGIENGYDRFLELVSRGRKMPKEVVDKVAQGQVW
                     LGTDALKHGLVDELGNFNVAYNALSELINQKREAENQPKVEQFGLQWFAEQDDSLFGT
                     LARDFKAQLQFSVANWLDLPLLKQPQQQLETLSRFNDPKQMYLYCLNCGKID"
     gene            10041..10604
                     /locus_tag="PXE44_000834"
     CDS             10041..10604
                     /locus_tag="PXE44_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL5588364.1"
                     /translation="MQILDLLHHRRSNKKFGDKAPNAEQLEQILKAGLRVPDHGRLKP
                     YRFVVIEKSGMPQFGECLKSAVAEFDMGEERLKKAEKLANQAPMIIGVVATLDDSIAK
                     VPSWEQMITAGCATYAIQLAANAQGFDTVWITNKWINGSALRSAFGCQESDKIIALIM
                     LGSPAEGEQIALAPESESLEGFVRYLK"
     gene            complement(10675..12999)
                     /locus_tag="PXE44_000835"
     CDS             complement(10675..12999)
                     /locus_tag="PXE44_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL5588365.1"
                     /translation="MTDLNLKISQIIAGELNVGSHQILAAITLLGEGNTIPFIARYRK
                     EVTGGLDDTQLRHFETRLIYLREMDERRQTILKSIEEQGKLTDELKAKILSSESKTEL
                     EDLYLPYKPKRRTRGQIAIEAGLEPLADSLWNDPKQDPETLAANFVDAEKGVADTKAA
                     LDGARYILMERFSEDAELLAKLRQYLTAYATLESKVIDGKEEEGEKFRDYFAHSEPFN
                     SVPSHRALAMFRGRNEGVLSLSLNADPTAEEGTKSSHCEEIIREHLGVIFNNQPADKW
                     REQVIAWTWKIKAALHLETELMATLCEKAEEEAIDVFARNLSALLMAAPAGARNTMGL
                     DPGLRTGVKVAVVDNTGKLLDTATIYPHTGREAEAQLAIFSLIKQHNVELIAIGNGTA
                     SRETERFAKAVIKEIKENKPQTVVVSEAGASVYSASEFAANEFPNLDVSLRGAVSIAR
                     RLQDPLAELVKIEPKAIGVGQYQHDVNQSQLARKLDAVVEDCVNAVGVDLNTASAPLL
                     ARVAGMTKTLAQNIVAFRDENGRFTSRSELKKVPRLGPKAFEQCAGFMRIIGGKNALD
                     ASSVHPEAYPVVEKILQATTSTLAELMGNNSKIHSLNAKDFVDEQFGLPTVNDIFKEL
                     EKPGRDPRGEFKTAVFMDGVEEITDLKAGMILEGTVTNVANFGAFVDIGVHQDGLVHI
                     SMLSNSFVEDPHTVVKAGDVVKVKVLEVDAARKRIALTMRLDDKPTDKKEPTSGRNSA
                     EICKNTQKNDRLQSNNRNAFGNNAFADALKGWKK"
     gene            complement(12999..13553)
                     /gene="pilW"
                     /locus_tag="PXE44_000836"
     CDS             complement(12999..13553)
                     /gene="pilW"
                     /locus_tag="PXE44_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HDL5588366.1"
                     /translation="MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARIN
                     LALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLAYYYQQTGDAENAEKAYQTALKL
                     SQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
                     QKIAETLTELEKLDKPRANLLKEN"
     gene            13773..14024
                     /locus_tag="PXE44_000837"
     CDS             13773..14024
                     /locus_tag="PXE44_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5588367.1"
                     /translation="MKIVVFQRKALKQLRKIPTGAEIRRKCDDLAYFPYCANIKALTN
                     HQYDYRFRVGNYRIFFNIEGETMNIVSIEEVKKRDERTY"
     gene            14008..14355
                     /locus_tag="PXE44_000838"
     CDS             14008..14355
                     /locus_tag="PXE44_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5588368.1"
                     /translation="MKELTNIQYLNNEEGKPAFAVLPIATLEWLKQKANIKTAIETGI
                     PSAVAGIALDNNYSALRAWREYLGLTQEEVAQRLGISQSAYSQHENAKNLRKTTRIKI
                     AYALGLNEEQLDF"
     gene            complement(14396..15556)
                     /gene="trmG/rlmN"
                     /locus_tag="PXE44_000839"
     CDS             complement(14396..15556)
                     /gene="trmG/rlmN"
                     /locus_tag="PXE44_000839"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDL5588369.1"
                     /translation="MSELITNAGCGSAEQPKEKINLLNLNRQEMRELFAEMGEKPFRA
                     DQLMKWIYHFGEENFDNMSNINKVLREKLKRIAEIKAPEVAVEQRSADGTIKWAMQVG
                     DQQIETVYIPEDDRATLCVSSQVGCALACTFCSTAQQGFNRNLSVAEIIGQVWRASKI
                     IGNFGVTGVRPITNVVMMGMGEPLLNMNNVIPAMEIMLDDFAYGLSKRRVTLSTSGVV
                     PALDKMREQIDVALAISLHAPNDELRDELVPINKKYNIKMLMDSVNKYREVSNANHGK
                     VTIEYVMLDHVNDSTNHAHQLAEVLKNTPCKINLIPWNPFPEAPYGKSSNSRVDRFQK
                     TLMEYGFTVTVRKTRGDDIDAACGQLAGDVIDRTKRTLEKRKFGQGIAVKTH"
     gene            15816..16712
                     /locus_tag="PXE44_000840"
     CDS             15816..16712
                     /locus_tag="PXE44_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL5588370.1"
                     /translation="MKLSTIKKALVLTFGLSAAMAMAEPFKVVTTFTVIQDIAQNVAG
                     DKATVESITKPGAEIHDYQPTPKDIVKAQKADLVLWNGMNLERWFERFFENVKGKPAV
                     VVTEGITPMPITEGEYKNLPNPHAWMSTDNALIYIENIRAALVKYDPQNADTYNANAK
                     AYAEKVKGIAEPLRQRLAFIPEQQRWLVTSEGAFSYLAKDYNLKELYLWAINAEEQGS
                     PKQVKKVIDGVKANKIPVVFSESTVSDKPAKQVAKETGALYGGVIYVDSLSEKDGPVP
                     TYLDLLKVTIGTIVEGFERSKK"
     gene            16725..17468
                     /locus_tag="PXE44_000841"
     CDS             16725..17468
                     /locus_tag="PXE44_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL5588371.1"
                     /translation="MTTVSASISVENLSVRYNNGHIALQDVSFHLENGTICALIGVNG
                     GGKSTLFKSLMGLVKPLTGSVKLSQLPIAKALKQNLVSYVPQSEEVDWQFPVSVYDVV
                     MMGRYGYMNFLRRPSQTDKLKVEQAMQRLDVLHLKDRQIGELSGGQKKRVFLARALAQ
                     ESKIILLDEPFTGVDVKTENAIVELLKQLKNEGHLILVSTHNLASVPSFCDQVLMVNR
                     TLLAKGKTEEIFNNQNLERVFGGLLHYQK"
     gene            complement(17525..18334)
                     /locus_tag="PXE44_000842"
     CDS             complement(17525..18334)
                     /locus_tag="PXE44_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL5588372.1"
                     /translation="MLNWILEPLQFEFMQIALITAILVAGVSAVLSCYLVLKGWSLMG
                     DAISHAVLPGVVISSLAGLPLSIGAFFSGLGCALSVGYLKENSRLKEDTVMGIVFSGM
                     FAFGILLFTAFNTGEHLTHILFGNLLGVTKSDLLQTLLISLFTFVVMVAKRKDFLLYC
                     FDVNQAKVAGLPVRLLHYGLLSLLALTIISAMQVVGVILVVAMLITPGITAYLLTKRF
                     DSMLLIALGVAIFSSVFGTLLSYHIDAATGPTIILVQAAIFFLSFAYSKIH"
     gene            complement(18327..19187)
                     /locus_tag="PXE44_000843"
     CDS             complement(18327..19187)
                     /locus_tag="PXE44_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL5588373.1"
                     /translation="MLDLLLEPFTYNYMQKAIFVSMGVGLVCAFLSAFLMLKGWSLIG
                     DALSHAVVPGVAIAYALKLPYAFGAFFSGILAALSILWVKSLTKIKEDAVIGFIFTTF
                     FALGMFIVSLNPTSVDVNSIVMGNILGIADDDLWQVVLIIAISLLGLILFWKDLLLVF
                     FDEHHALSVGLSPLRYKVIFFTLLSACVVVALQTVGAILVIAMVVTPGATAYLLTDRF
                     PRLVLIAMLIGTLTSGIGAYLSYFLDGATGAVIVCLQTLIFLFAFCFAPKYGLITQKR
                     KLHRVEVQNA"
     gene            complement(19261..20046)
                     /locus_tag="PXE44_000844"
     CDS             complement(19261..20046)
                     /locus_tag="PXE44_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDL5588374.1"
                     /translation="MMKKITILGLGWLGLPLAEALQNKGWRVAGTKRTVGELAIECYP
                     LDLNALAVTSEIEQLLAADALVIALPLSQIRPENYGCGIQTLVSLAIEKGLKHLVFTS
                     STSVLPMKSGVFDENAVVDSANLLVKMENWLLSQPIHCDILRLAGLVGKKRHPVFYLA
                     GKQNLSGAGQPVNLVHLEDCIAAISLLLEQPNGQRIFHLCAEQHPSRKDYYSEMAKRF
                     GLADLHFSEENQPLVRLIKADKICQELGFRYYYPDPYQFKVDI"
     gene            complement(20134..20209)
                     /locus_tag="PXE44_000845"
     tRNA            complement(20134..20209)
                     /locus_tag="PXE44_000845"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20174..20176),aa:Gly,seq:gcc)
BASE COUNT         5810 a         4541 c         4267 g         5649 t
ORIGIN      
        1 acacaatata ttgtgttaat gaattagtaa tggaaaatct cagattcagc tcggaacaaa
       61 ttgtttggca agaagtgccg aatgagacct cacttgcttt tctgattaca ggctgcccgc
      121 ttggctgtaa aggttgccat agtgcggata gctggaaagc ctgtcttggg aaagagctta
      181 gcagtgaata tctctctgaa cgcctaaaac gctaccaagg cttaattagc tgcgtattgt
      241 ttatgggcgg agaatgggaa gcagaaaaat tactgccgct gttgcaactg gcaaaaaaac
      301 aggggctaaa cacctgtctt tatagtggtt tggagcagca cgaaatcgcc gagactttat
      361 tacacgagct aacctactta aaaaccgggc gttggattgc cgaacgagga ggattggaaa
      421 gtttaaccac caaccaacgc tttactgatt tacgcaataa ccaaaatctt aaccatcttt
      481 ttattcgagg agcttcaata tgattcgctt acaaccgaca caattagacg gaaaactaca
      541 tttcattaac caatatattc aagccaaaaa tgccgcagac ggttcaaaaa tggacgccaa
      601 cgctaacgtc acgcaaaaaa acatagcgac aatggaacag gagctaatga aagatttttt
      661 tgtgcaaatc aaccgagcaa aagtgagtga aaaaattgca aaaatttttg gaaaagaaac
      721 cgcttgtgaa tatttacgtc aaatcgaagc acacgaaatt tacgtacacg atgaaaccag
      781 cctaaaaccc tactgcgttt cagtcactat gtaccctttc ttgcgtgatg gtttgactaa
      841 actcggcggc gaatccaaag ccccgaaaca tttagcctca ttctgcggtt cttttatcaa
      901 ttttgtcttt gcagtcagtt ctcagtttgc cggtgcagtc gccacggttg aatttctgac
      961 ctattttgac tattttgccc gcaaagatta cggcgataat tacttacaga ctcatcgttc
     1021 agatattgaa aaccacctgc agcaggttgt gtatagcatc aaccaaccgg cagccgctcg
     1081 tggttatcaa agcgtctttt ggaatatttc agtgtatgac aaattctatt ttgaagcgat
     1141 gtttagccgt tttgtttttc ctgattttga gaaaccaaat tgggaaacca cttcaaaatt
     1201 acaacttttc tttatgcaat ggttcaataa agagcgaagc aaagcgattc tcactttccc
     1261 tgttgtgacc gcagcaatgc ttaccgaaaa gggcaaatgt aaagatgacg agtttgccaa
     1321 tcaaatggca aaagagctat ctcaaggcaa ttcattcttt atttatcaat cagataatcc
     1381 ggattccctt gcttcttgct gccgcttgag aaatgaaatc agcgatcata gcttctccta
     1441 ttcattaggg gctggaggtg ttgcgacagg ttcaatcaat gtaattacta ttaatatgaa
     1501 ccgtttggtg caagataacc gtgatttaag cgaagaagtc agcaagatcc ataaatatca
     1561 atacgcttac cgcaaactga tggaagagta tttagctgcc ggtatgttgc cggtttacga
     1621 tgccggtttt atttcactgc ataaacaatt cctgactatt ggcattaacg gaatggcaga
     1681 agctgctgaa tcacaaggtt taaccgtagg ttacaatcca gactatattg attttgtgca
     1741 atctcggtta aaaattattt tccaagctaa ccaagcagcc ggtgaaaagt atggggtaaa
     1801 atttaatacc gaatttgtgc ctgctgaaaa tttaggggtc aaaaacgcca aatgggacaa
     1861 agaggacggc tatttcgtac cacgagattg ctacaattcc tatttctatg tggtagaaga
     1921 tgaagaaatt aatgctttgg ataaattctt actgcacggc agagagctga ttgaatggct
     1981 ggacggaggc tccgctctac acttgaattt agacgaagcc ttaacccaaa ccggatatcg
     2041 ctccttgctg gataccgccg ctaaaaccgg ctgcaattat ttttgtatta acgttaaaat
     2101 cacgatttgc aacgaatgta gccacatcga caaacgcacg ttgcacagtt gtagcaaatg
     2161 tggctccgat aatattgatt acggcactcg ggtaatcggc tatttaaaac gagtctccgc
     2221 ctttagttcc gcccgccaaa aagagcacag cttgcgtttc tatcaccgag aagcagctta
     2281 attgaaaact gcagttgtag ccctttacaa ccgctttttt agtgattata ttaaagcctc
     2341 atttagtcgg ggtgcttgcc taagggcaag ctgagaaata cccgttgaac ctgatgcagt
     2401 tagcactgac gtaggaaact aaaggcgtaa acccatctat tccccgttgg tgttggtgcg
     2461 ttgtttttca atcatattaa ggaataaacg atgaccacat cactccccta tcactcgcat
     2521 tatttgcaaa aaattcgaca aaatcgaccg cttgttcaca acattaccaa cattgttgcc
     2581 gctaattttt ctgccaacgg cttattggct cttggtgctt ctcccattat ggcagctgct
     2641 attgaggaga tggaatccgt gccggcactc agtaacgccg tggtgattaa tatcggcacg
     2701 cttatcggca aagaagtaga agcaatggag cttgctggca aaaccgccaa ccgtttaggt
     2761 attccgctca ttcttgaccc tgtcggcgtt ggagcaacac ctttccgccg tcaaacagtt
     2821 gaaaccttag tcgaccaaat tcacttcacc gccattcgtg gaaatgcggg ggaactagct
     2881 accctcgccg gtgtagaatg gcaagccaaa ggcgttgatg ccggcgaagg ctcgcataat
     2941 gtggttgaaa tcgcccaaca gattgccaaa cgttaccaaa ccttagtcgc aatcagtggt
     3001 gaaaccgatg tggtaagctc tggctcacac acagctttat tgcacaacgg cagcccgatg
     3061 ttccccaaaa ttaccgcttc aggctgttta ttaagtgcgg tatgcggggc attcttagcc
     3121 gttgccgaca aagagcacta tttcaacgca cttattgagg cttgcgccgc ttatgccgtt
     3181 gcaggcgaag tggctgctca agcactcaaa ccaactcaat acgggcaatt tgccgtcaat
     3241 ctgctggata cccttggggc attaacgcct gaaaccgttg agcaatatgt gagggttgaa
     3301 tatgtctaaa actcactcaa acccaactaa agtggcggtt gcctcaatgg tcggcacggc
     3361 gattgaatat tttgataact acatctacac gatggcagca gtgctggtgt ttaatgtgca
     3421 gttttttaat agcgatgatc cggtttccaa ccaattaatg tcgctttccg tactagctct
     3481 tgcctttttc acccgcccga tggggtcgat tttattcggg catttcggcg ataaatacgg
     3541 acgcaaacaa actctgattg cctcgctctt attaatgggc ttatctaccg tagcaatcgg
     3601 gcttttgcct aattatgccg ccattggcat ttgggcaacc gtgttactct gcctctgtag
     3661 aatcggacaa ggtttaggct taggcggcga atggggcgga gctgctctcg ttgccactga
     3721 acacgccccg aaacacaagc gggcattttt tggtattttt ccgcaaatgg gcgccccaat
     3781 cggcttgctg ttggcaaacg gagcgttcta cttggtgaat ttaatttacg gtgaacaaac
     3841 cttcttggat tgggcgtggc gaattccgtt tgtcgcttct attgtgatga tcgccattgg
     3901 gctttatatc cgaattaaga tcagcgaaag cccgattttc cttaaagcgg aacaacaagg
     3961 taaaaaccgc cataccccgc ttaaaacttt gtttagccaa cacctcaagc cttttatgat
     4021 cggcgtgctg attgccaccg ccggctatgt gttgttctac attttagttg ctttcacccc
     4081 gattttcgtc aaaagcccgg tggcagtttc tcaagcagga cacgctacag gcttaggttt
     4141 accggtaaat ctctacaccc aatatttgct gattacctcc gtgattttcg gcattgcgat
     4201 tttcttctcc ggcatttatt cagacaaaat cggacgccgt ttactgctgt tacttgccac
     4261 aggagcaacc gtagtttacg gcttaacaat gccgtttttc ctcgaaaacg gcacaccgac
     4321 ttcgatcttt attttcctca ccattggaat gatcttaatc ggcttaagct tcggcccgat
     4381 ggcagtaatt ctacccgaac tcttcccaac agaagtacgt tacacagggg cttcgcttgc
     4441 ctacaccgct gccggtattt taggagctag cgtgttcact attattgcgg tcaaaatcaa
     4501 tgaaaatttc ggcttattcg gtgtaggcgg ctatttatcg gttgcctcat tactgagctt
     4561 ctgggcattg tggcaagtcc acgaaaccaa agatttggaa ttagaggaga tataaaaaac
     4621 cactcactta acgacggggc aagttaccac cttgccccaa gatgagggaa tgcaaatttc
     4681 cctctaatag acttcacaac ggatattttg cattttttta taaaaaataa ccgcttgtaa
     4741 gctatactaa aaacgcttgt cggagtgccg taaggctgag atcgcaacag cgagatccgt
     4801 ggaacctgta ggttagtacc tgcgtaggga acaagtttta cccaaattca gattttggga
     4861 tcattaccac atctctggca agcaaaaccc taagcatatt atgaggattt tgcgatgtct
     4921 aattattcaa actctccata ccaacctaac gtcatttgga cggttgccgg ctcagattcc
     4981 tgtgccggtg ccggtctgca aaccgattta catacctttc acgattttaa cctagtgggt
     5041 tgttcagtag ttacttctgt aacggctcaa catccgcacg gtgtgctgtg tgtaacgcct
     5101 gttgatgatc acaccttccg ccaacagttt gaagccttgc tggttcaagg ctacccgaac
     5161 gcaatcaaaa tcggcttact ctgctcgcaa gcacaagtag agatcttgtg tgaatatatc
     5221 caaaaaatcc gtgcagaaag ctcgcattat tgttatgtgg tttacgaccc tgtggcagtg
     5281 gcaagcagtg ggcaggcgtt atccgacagt attttattgc agatagtgca acaaaaattg
     5341 tatccgctgg tggatttaat cactccaaac ggcactgaac tcgctttatt aagcgacaca
     5401 gaaatccaga cctttggtga tgtgaaaacc gctagccaca aactgtttgc acagggaatc
     5461 aaagccgtgt tagccaaagg tggacatttt gaatggctgg gggaaacggt aagagattat
     5521 ctgctgactg caaatgaaat ttatgccttt agtcacgccc gacagcaaag tgtgaatacc
     5581 catggcacag gttgcaccta tgcttcagcg gttggagcga tgttggcaac cggctttgat
     5641 ttagctgatg ccgtcactgt tgccaccgct tatttgcaaa aaggcttatc ggaaaaacaa
     5701 ggcagagggc aaactgcttt aagttcactt tgccacaccg gctaccctga tgatatcgct
     5761 ttcttcccac aaaccgagct gatttctgcc cctcttaaat tgccgaaaga gccttttgct
     5821 ccaacggaat atcaacttgg gctttaccct gttgtagata gcgttaaatg gcttgaacgc
     5881 ttaattcttg taggggtaaa aaccttgcaa attagaatca aaaatcgacc gcttgcagag
     5941 attgagcaag aaattgccca ttcggtcgca ctggcgaaac agcacaatgt gcgtttgttt
     6001 gtaaatgatt actggcagtt agccatgaag tatcaggctt acggcgtgca tttagggcag
     6061 gaggatttag ccaccgccga tttaaatgca attcagcaat ctgggttacg gcttggtgtt
     6121 tcaacccact gctactttga aataatgcgg gcattggcgg tgaaaccgtc ttatatcgct
     6181 tttggccacg ttttccctac tcaaaccaaa gtaatgccgt ctcaaccaca aggcttaaca
     6241 aatttagcca aatatgttgc tctcgttgca ccgcttaaca ttccaactgt ggcgattggt
     6301 ggaattaatg agcaaagcat tgaaaatgta atgcataccg gtgtcggcag tgccgcggtg
     6361 gtaagtgccg ttacccaagc aaaagattgg caacaagcgg tcaaaaactt agcaaaattt
     6421 gcaaatggag gccgtaatga atagaactct tagtacgcta aacagtggtt tgatttgggc
     6481 gggagcgggc attagtgcca gtgcaatcgt ggttggcact tggattgccc cacttggctg
     6541 gcaacaaggc ttgctggcga tagtgctagg gcatttatta ggcggcattt tgttttttgc
     6601 tgccggctta attggggcga aaaccggcaa aaatgcgatg caaacggtgc aaatttcctt
     6661 tggcagaggc tcggtgtttt tctcccttgc caatgtgcta cagcttgttg gttggacagc
     6721 cattatgatc tatacagggg cagagatcag taatgccctt agccttgcct tatgggaata
     6781 ttcggctttc tcgctttggg caatcgtgct tgggttgctg attgtgctat ggttatttac
     6841 cggctctaat caggtcggaa aattcaagtt agtgacattg atagcgatgt ttttgctcac
     6901 cttatggcta agcattaagg tattgaatgg gcaggaaaat tttcctgttt cgggtgaaat
     6961 gccgtttagc accgcagtgg aattagcaac tattatgcct ctttcgtggc tacctttggt
     7021 atcagactat accgctaaag cgaaaaagcc ttttgccgcg accctttcgg cgaccatagg
     7081 ctatttgatc accagtgcgt ggatgtatgc gttaggtctg ggtatggtgc tatttaccgg
     7141 gcaaaccgaa attgccccta tgttactaat gacaggaatg agccttatcg gcattatggt
     7201 gatgattctt tcgactgtga ctaccacgtt tcttgatgcc tattccgccg gtgtcagtgc
     7261 cggcaatatc acggcaaaat tcaaagaaac cccaaccgcc attctagtca caatcatcgg
     7321 cacgctactg gcaatcagtt taccggttac ccaatatgaa aatttcctgc tgctgatagg
     7381 ctcggtattc gccccgatga ttgccgtgca aattgcggat ttctttgtgt taaaacgcca
     7441 ccaagcacag cctaatgttg attgggttgc tgccggatta tggcttgtcg gcttcgtgct
     7501 ataccacctt ctctcttacc accaagtgca tttactactc ggcttaacca ttccagtgat
     7561 gttagtgatt tttctcagca ccatttttgt gaggaggttc acaaaataga aagataagcg
     7621 gttcaattgg agtgaaaatt tgctaaaatt gagtcgcttg ttttaaacaa tgactgggta
     7681 gtaacaccac aagctcaact tgcctaccac accattaacg gtaaagccga tgaggaacgg
     7741 ttaaatcttt tagttgcccg tgccggcgta agagtagcaa aaggctttgc cttgaataat
     7801 ggttggaatc tgcaacctta tgcggaagta aacggcattg ctgaaaaagg ccaatagtac
     7861 gaaagtgcaa gtaaaccaat atcagtttgg agttgccgaa aacaagggca gagtgcaagg
     7921 ttcacttggc ttgagtgcag gaaacggtaa tcaccgttta ggtttagagg catcagttgc
     7981 ttcaggtaag aaactgaaac aaccatttaa tgtattagta aattatcgtt atcaatggta
     8041 atttaagagg gcaggtgaaa acctgccctt aaattcaata attaatctat cttcccacaa
     8101 tttaaacagt aaagatacat ctgtttcgga tcgttaaaac gtgaaagagt ctctagctgt
     8161 tgttgcggtt gcttcaacag cggtaaatca agccaatttg ccacgctaaa ttgtaattga
     8221 gctttgaaat cacgggcaag cgtgccaaat aagctgtcat cttgctccgc aaaccattgt
     8281 aagccgaact gttcgacttt aggttggttt tccgcttcac gtttttggtt gatcaattca
     8341 gataacgcat tatatgccac attaaaattg cccaactcat caaccaatcc gtgttttaac
     8401 gcatcagttc ccagccacac ttgcccttga gccactttat ctaccacctc ttttggcatt
     8461 ttcctgccac gagaaaccag ctctaaaaaa cggtcgtagc cgttttcaat cccaatttgc
     8521 agcacttctg cctgctcttg cggtaggctt tttaaaccgg cttgttgtgc tagggcagaa
     8581 gtcgctatgc cgtcttcgct cacaccgaga tttttagcgg ttttttcaaa agttactgct
     8641 aaaccgaaaa taccgataga gccggtaagc gtattcgggc tagcaataat tttatcgctg
     8701 gtagccgcaa tccaataacc gcccgatgct gccattccgc ccattgaagc aaccaccggt
     8761 ttgccggctt gttgaaaggc ttccacttct tggcgaatca gttcagacgc cacagcactg
     8821 ccgccggggc tgttaatgcg tagaattaag ccttccacat ttttgtcatc tcgagcttgt
     8881 tgcagtaatt ttaccagcgt ctcgctgccc acattcattt cgtcactttc accgccagca
     8941 atcgcccctt ccacattcac taccgcaatt ttcggagcat tcacttctgc aaagcggtcg
     9001 tttagttcag tcatataatc taaaaattca atctggttat aactgccttc gctgtttttg
     9061 ccaaactgtg caatcagttt atcccgactt tcttggctgg acactaactg actaacccat
     9121 ttttgattta atgccagttg agcatcatct cctttagctt gtttatattt ttcaatgtaa
     9181 ttggcaggcg ataaatccag tgcttgcggt tcaatgttgc ggttttgagc tagtgttgcc
     9241 acagtttgtt gccaagtgcc gtttaaccag cctcgggcat tttgtttcgc ctcctcagac
     9301 atatcatcac gcataaacgg ctcaaccgcc gatttataag taccgacacg gaaaatatgc
     9361 ggttcagcct caattttatc gagcaaggtt ttgaaataag tgttggaata atttaagcct
     9421 tttaaatcca ccgcaccgac tttattgaga taaatttcat cagcaaagga agcaagataa
     9481 tattgttttt gcgagtagtc ttcgcccact gcaatcaccg gcttgcccga ttgtttgaag
     9541 ttgttgattt cattgccgat aaaatccaat gagctgaaat cagcactttg tagcttttgc
     9601 agatctaaca ccaagccggt aattttcggg tcatttttag cgtggctaat cgcttgcacc
     9661 acatcaaaag tcgagatttt aaacggctcg gaactaccta attccgattg caccaagcgg
     9721 tagaaatcag tgaattcttc ccgattatct gctaaataac catctaaatt taaacgtaac
     9781 gccccttgat tgaacaccgg cttttgcgtg ctgccagaag agctgataaa tgcgaccacc
     9841 ggcactaata gcaaaataaa cagaataaaa aagagattaa tgacacactc tctaatacaa
     9901 cggaagattc ggtaaaatgc ttttaaaatc gttaacatcg gaaattccta aaaataaact
     9961 cagtcggcac agtataaagc aatcgctaaa tgaagaaaat atgctattct tgaacgcaaa
    10021 aaattaacaa aggaacgctt atgcaaatct tggatttatt acaccatcgc cgttcaaata
    10081 aaaaatttgg cgataaagcc ccgaatgcag aacaactgga acaaattcta aaagccggct
    10141 tgcgagtgcc ggatcacggg cggttaaagc cttatcgctt tgtggtgatt gaaaaaagcg
    10201 gtatgccaca gtttggtgaa tgcttaaagt ctgcggtagc agaatttgat atgggcgagg
    10261 aacgcttaaa aaaagcggaa aaattagcta atcaagctcc aatgattatc ggcgtggtag
    10321 caacattaga cgacagcatc gccaaagtgc caagttggga acaaatgatt accgcaggct
    10381 gtgcaactta tgcgattcag ttagcggcaa atgcacaggg ctttgatacg gtttggatca
    10441 ccaataaatg gattaacggc tctgctcttc gctccgcttt tggttgccaa gaatctgata
    10501 aaatcattgc cttaattatg cttggctcac cggcagaggg cgagcaaatt gcattagccc
    10561 ctgaaagtga aagtttagag gggtttgtac gttatcttaa gtaagcaggt taacagataa
    10621 aagttagtaa taagggcgaa aaatatttcg cccttgcttt tatctgtttt cgccttactt
    10681 cttccagcct tttaacgcat cggcaaaggc attgttacca aaagcgttgc gattgttgct
    10741 ttgcaagcgg tcatttttct gcgtattttt gcaaatttct gctgaatttc gaccgcttgt
    10801 tggctctttt ttatcggttg gtttgtcgtc taaacgcatc gtaagggcaa tacgtttgcg
    10861 ggcagcgtcc acctcaagca ctttgacttt caccacatca ccagctttca ctaccgtgtg
    10921 cggatcttca acaaaagagt ttgagagcat tgaaatatgc accaagccat cttggtgtac
    10981 gccaatatcg acaaaagccc caaagtttgc cacattggtc actgtgccct ccaaaatcat
    11041 accggctttg aggtcggtaa tttcttccac gccgtccata aacacggcgg ttttaaactc
    11101 tccacgaggg tcacgccccg gtttttctaa ctctttgaaa atatcgttca ccgttggtaa
    11161 accaaactgc tcatccacaa aatctttggc gttgagcgag tgaattttgc tgttgttacc
    11221 cattaattct gcaagtgttg aggtagttgc ctgtaagatt ttttctacca ccgggtaagc
    11281 ttccgggtga acgcttgagg catcaagggc atttttccca ccgataattc gcataaaacc
    11341 ggcacactgt tcaaaggctt tcggccctaa acgaggcact tttttcagct ctgagcggct
    11401 ggtaaaacgg ccgttttcgt cacggaatgc cacgatattt tgtgccaatg ttttggtcat
    11461 tcccgccacc cgagccagca acggagcgga ggctgtattc agatccacgc ccactgcgtt
    11521 tacacaatct tctactaccg catcgagttt acgggcaagt tgggattggt ttacatcgtg
    11581 ttgatactgc ccaacaccaa tcgcttttgg ttcgattttc accaactcgg caagtggatc
    11641 ttgcaaacgg cgggcaatcg aaaccgctcc ccgtaatgaa acgtctaaat ttggaaactc
    11701 gttagccgca aattcagagg cagaatagac cgatgcccca gcctcactca ccaccaccgt
    11761 ttgcggttta ttctctttga tttctttaat caccgctttg gcaaaacgtt cggtttcacg
    11821 agaggccgta ccgttaccaa tcgcaatcag ttccacattg tgttgtttga ttaagctgaa
    11881 aatcgcaagc tgtgcctccg cctctcgccc agtgtgaggg taaattgtgg cggtatccag
    11941 caatttaccg gtgttatcca ccaccgccac tttcacgccg gtacgcaagc ccgggtctag
    12001 ccccattgta tttctcgcac cggcaggggc tgccattaat aatgccgaga gattgcgggc
    12061 aaacacatca atcgcctctt cttcagcttt ttcacacagg gttgccatta attcggtttc
    12121 taaatgcaga gctgctttga ttttccacgt ccaagcaatc acttgctcac gccatttatc
    12181 tgctggttga ttattaaaaa tcacgcccaa atgctcacga ataatttctt cacaatggct
    12241 ggattttgtg ccttcttctg cagtcggatc ggcatttaat gaaagtgata acaccccttc
    12301 atttcgccca cggaacatcg ccaaagcacg gtgggaaggc acgctgttaa acggctcgct
    12361 atgagcaaaa taatcacgga atttctcgcc ctcttcctct ttgccgtcaa tcactttcga
    12421 ttccaacgtg gcgtaagcgg tcaaatattg gcgaagtttt gcaagtagct cagcatcttc
    12481 gctaaaacgc tccattaaaa tatatcttgc cccgtctaat gcggccttgg tatccgcaac
    12541 gcctttttcc gcatcaacaa agtttgccgc taaggtttca ggatcttgtt tcggatcatt
    12601 ccataaactg tccgccaatg gctctaaccc tgcttcaatc gcaatttgcc ctctggtgcg
    12661 acgttttggt ttatacggca aatagagatc ttccagctcg gtcttgcttt cactgcttag
    12721 aattttggct tttaattcat cggttaattt gccttgttct tcaatcgact tcaaaatggt
    12781 ttggcggcgt tcgtccattt cacgcaaata aattaagcgg gtttcaaaat ggcgaagttg
    12841 ggtgtcgtct agcccgccgg tcacttcttt acggtaacga gcaataaatg gaatggtatt
    12901 gccctcacct aatagcgtga tggctgcaag aatttggtgt gagccaacgt tgagttcgcc
    12961 ggcaataatt tggctgattt ttagatttaa gtcagtcatt agttttcctt caataaattt
    13021 gctcgaggtt tatccaattt ctcaagctcg gtcaatgttt cggcaatttt ttgcagattc
    13081 ggctcttttt tcgcacataa caccatattt tctaagctat ccgcttggtg atagtagggt
    13141 gctttgcttt tgacagcctg ctcaaattgc tgataagcct gttgaaattg accctgttta
    13201 cataaaaaag tgccgtaatt attcagcaca tcagggcggt tttgactcaa tttcagtgcc
    13261 gtttggtagg ctttttcggc attttcagca tcacctgttt gttggtaata ataggcaagc
    13321 accgaataag ggaggtaatc tttttcatcg tgagccaagg ctttatcaat attctgctta
    13381 gcttttggga aatcattttg ctccagataa gctaaagcca aattaatacg ggcttttacc
    13441 gcttccgagc ggttaaattc gacttcagag gaagaagggg gaagagaaga acaagccgct
    13501 aataaaaaaa tcagaataca agcggtcatt tttggaaaaa attttgcaaa cataacgttc
    13561 tcctacaaca aagggcttaa ttttactgtt ccatcctcgc taagactagc gattttttgc
    13621 gaagaagaaa aatttttcgt tttcattttt cgacgcagat cgcaaaaata ataatggggt
    13681 gttgtgctag ggtaaatggt aggaatacac tctctattta atataagaat ttcattgaaa
    13741 aaacttataa aacttataaa atagaggcaa aaatgaaaat agtggtattc cagcgtaaag
    13801 ccttaaagca attacgaaaa atcccaactg gtgcagaaat tcgccgaaaa tgtgatgatt
    13861 tagcttactt tccctactgt gccaatatta aggcattaac aaatcatcaa tatgattatc
    13921 gctttcgggt cggaaattac cgtatttttt tcaatattga aggcgaaacg atgaatattg
    13981 tgtctatcga agaggttaaa aaacgagatg aaagaactta ctaatattca atatttaaat
    14041 aatgaagaag gaaaacctgc ctttgccgta cttccgatag caacacttga atggcttaag
    14101 cagaaagcaa atattaaaac cgcaatagag accggtattc catctgctgt tgctggaatt
    14161 gcattagata ataactactc agctctgcgt gcttggcggg aatatttagg tttaactcaa
    14221 gaagaggtgg cacaacgctt ggggatttcg caatcagctt atagccaaca cgaaaatgca
    14281 aaaaatctac gaaaaacgac ccgcataaaa attgcctatg cgttgggttt aaatgaagag
    14341 caattagatt tctaataaca aaaaggcagg aacctctgcc ttttcactat tcagattaat
    14401 gggttttaac cgcaatacct tgcccgaatt tacgtttttc aagggtgcgt ttggtgcggt
    14461 caatcacatc gccagcaagc tgtccgcaag cggcatcaat atcatcacct cgtgttttac
    14521 gcactgttac cgtgaaaccg tactccatca aggttttttg gaaacgatct acccgagagt
    14581 tagagctttt accataagga gcttctggga acgggttcca cggaatcaag ttaattttac
    14641 acggcgtgtt ttttaatact tcagcaagtt ggtgggcgtg gtttgtgctg tcattgacat
    14701 gatctaacat cacatattca atcgtgacct tgccgtggtt ggcgttagaa acttcacggt
    14761 atttattcac cgaatccatc aacattttga tgttgtactt tttattaatc ggcactaatt
    14821 catcacgcag ttcatcgttc ggagcgtgaa gtgaaattgc caacgccaca tcaatttgct
    14881 cacgcatttt gtctaatgcc ggcaccacac ccgaagtgga aagcgtgaca cgacgtttag
    14941 ataagccgta agcaaaatca tctaacatga tttccattgc cggaatcacg ttattcatat
    15001 tcagcaatgg ttcacccatt cccatcatga ccacattggt aatcgggcga acaccggtca
    15061 cgccaaaatt gccgataatt ttagaagcac gccacacttg cccgataatt tccgccacgc
    15121 ttaagttacg gttaaagcct tgttgagcgg ttgagcagaa ggtacaagcc aaggcacagc
    15181 ctacttgtga agaaacacac agggttgcac ggtcatcttc aggaatatat accgtctcga
    15241 tttgctgatc gcccacttgc attgcccatt taatcgtacc gtctgcagaa cgttgttcta
    15301 ccgccacttc aggggcttta atttcggcaa tacgctttaa tttttcacgc aatactttat
    15361 tgatattgct catattatcg aaattttctt cgccaaaatg gtagatccat ttcattagct
    15421 ggtcggcacg gaaaggtttt tcccccattt ctgcaaacag ttctcgcatt tcttgacgat
    15481 taagatttaa taaattgatt ttttcttttg gttgttcagc agacccacag cctgcattgg
    15541 taatcagttc tgacataaag ttgaaagcct cgttattaca cggattacgg caaaaggtta
    15601 gggcaaatgc ccttattttg cgattggatt ttaaagcgtg caattttact ggctcttgag
    15661 ggattaggct agtaattttt aatcaaaaga gttgaattgc ctgagtaaac ttatcaagaa
    15721 taaccttata aaaacaagac tatttacatc attcccaatg ttctatagaa tagctttcga
    15781 taattctcat tctcaataac aaaaggaggc taaaaatgaa actatcaaca attaaaaaag
    15841 cattggtatt aacctttggt ttaagtgcag cgatggcaat ggcagagccg tttaaagtag
    15901 ttaccacttt taccgtgatt caagatattg cacaaaatgt cgccggcgat aaagccactg
    15961 tggaatccat tactaaaccg ggtgctgaaa ttcacgatta tcaaccaaca ccaaaagaca
    16021 ttgtaaaagc tcaaaaggca gacttagtgt tatggaacgg tatgaacctt gagcgttggt
    16081 ttgaacgttt ctttgaaaat gtaaaaggta aaccggcggt tgtcgtcacc gaaggcatca
    16141 ccccaatgcc gattaccgaa ggtgaatata aaaatctacc aaatccgcac gcttggatgt
    16201 ccaccgataa cgcattaatt tacattgaaa atattcgtgc ggctttagta aaatatgacc
    16261 cacaaaacgc agacacctat aatgccaatg caaaagccta tgctgaaaaa gtaaaaggta
    16321 ttgctgagcc actccgccaa cgtttagctt ttattcctga gcagcaacgt tggttagtaa
    16381 ccagtgaagg ggcatttagc tatttagcga aagactacaa cttaaaagaa ctttatttat
    16441 gggcaatcaa tgcggaagag caaggttcgc caaaacaagt gaaaaaagtg attgatggtg
    16501 taaaagccaa caaaatccct gttgtgttta gcgaaagtac cgtttccgac aaaccggcaa
    16561 aacaagtggc gaaagaaacc ggtgcattat acggtggcgt aatttatgtg gattcacttt
    16621 cagagaaaga tggtccggtt ccaacctatt tagatttgct taaagtgact atcggcacca
    16681 ttgtcgaagg gttcgaacga agcaaaaaat aataagattt agcaatgaca actgtttcag
    16741 cctccatttc cgtcgaaaat ttatcggtcc gttataataa cggacatatc gctttgcaag
    16801 atgtctcgtt tcatctggaa aacggcacaa tttgtgcatt aatcggcgtg aatggaggtg
    16861 gaaaatccac tctgtttaaa agtttaatgg ggctggttaa gccattaacc ggctctgtta
    16921 agctgagtca gctcccgatt gctaaagccc tcaaacaaaa tttggtttcc tatgtgccac
    16981 aaagtgaaga agtggactgg caatttccag tttcggttta cgatgtggta atgatggggc
    17041 gatacggcta tatgaatttt ctacgccgcc caagccaaac cgataagctc aaagtagagc
    17101 aagcaatgca acgccttgat gttttacatc tcaaagacag gcaaatcggc gaactttccg
    17161 gcgggcaaaa aaaacgggta tttttagccc gtgccttagc tcaagaaagt aaaatcatct
    17221 tacttgatga gcctttcacc ggtgtggatg ttaaaaccga aaatgcgatt gttgagctac
    17281 ttaaacagct taaaaatgag gggcatttga ttttagtttc cacccacaac cttgccagcg
    17341 taccaagctt ttgcgatcag gttttgatgg tcaatcgcac gctactggca aaaggtaaaa
    17401 cggaagaaat ctttaataat caaaatttag aacgggtgtt cggcggttta ttgcattatc
    17461 aaaagtagtg tttcagcaac aaatcacaaa caaataggga ggcaaatgcc tccctatttt
    17521 tgtatcaatg aatcttagaa tatgcaaaac tgaggaaaaa aatggccgct tgcacgagaa
    17581 taatcgtcgg ccctgttgcg gcatcaatat ggtagctaag caacgtgccg aataccgatg
    17641 aaaatatcgc aacgcctaag gcaatcagta acatactatc aaaccgctta gtcagcaggt
    17701 aagcagtgat ccccggggta atcaacattg ccaccactaa aatcacgccc accacttgca
    17761 ttgcactgat aatagttaaa gcaagcaagg aaagtaaacc ataatgcaat aatctaaccg
    17821 gtaagcctgc tactttggct tgattaacat caaagcaata gagcaagaag tctttgcgtt
    17881 tcgccaccat caccacaaag gtaaagaggc ttatgagtaa agtctgaagc aaatcgcttt
    17941 tggttacgcc taatagatta ccaaataaaa tatgggttaa atgctcaccg gtattaaagg
    18001 cggtaaacag caagatccca aaggcaaaca tacctgaaaa cacaatcccc attaccgtgt
    18061 cttctttcaa gcgactattt tcttttaaat agcccaccga taaggcacag cctaatcctg
    18121 aaaaaaacgc ccctattgag agtggtaacc cggcaagtga ggaaatcacc acaccgggca
    18181 gaacagcgtg cgaaattgca tcgcccatta aagaccagcc tttcagcacc agatagcaag
    18241 ataaaaccgc actcacgcca gcaactaaaa ttgcggtaat taacgcaatt tgcataaatt
    18301 caaattgcaa cggctctaaa atccaattaa gcattttgaa cctccacacg gtgcaatttc
    18361 cgcttttgag taatcaagcc atatttagga gcaaagcaga atgcgaataa gaaaattaag
    18421 gtttgtaagc acacaattac cgcaccggtt gcaccgtcta aaaagtagct taaatatgcc
    18481 ccaatccctg atgtcagcgt gccgataagc attgcgatca gcactaatct agggaaacgg
    18541 tcggtaagta aataagcagt tgctcccggc gtgacgacca tcgcaataac caaaattgcc
    18601 ccaaccgttt gcagagccac tactacacag gcacttaata aggtaaagaa aatcacttta
    18661 tagcgtaatg gcgaaagccc gactgacaat gcgtggtgtt cgtcaaaaaa gacgagtagc
    18721 aaatccttcc aaaataggat taaacccagt aacgaaatcg caataatcag taccacctgc
    18781 cacaagtcat catcagcaat gcctaaaata tttcccatta caatcgaatt aacatcaacc
    18841 gatgttggat tgagggaaac aataaacata ccaagcgcaa aaaaagtcgt gaaaataaag
    18901 ccgataaccg catcttcttt aattttcgtc aggcttttta cccaaagaat cgataatgcc
    18961 gccaaaatcc cggaaaaaaa cgcaccaaag gcgtagggca attttagtgc ataagcgatg
    19021 gcaacacctg gcactacagc gtgtgataag gcatcaccaa tcaaagacca gccttttaac
    19081 atcaaaaagg cggaaagaaa agcacaaact aaccccacgc ccatactgac aaaaatcgct
    19141 ttttgcatat agttgtaggt aaaaggctct aataaaagat caagcataaa atcctaacaa
    19201 gttgttgata atagttcgca tatactatca tataaactcc ttttcttgta tggattaaac
    19261 ttaaatatct actttaaatt gataaggatc aggatagtaa taacgaaaac caagctcttg
    19321 gcaaattttg tctgctttta tcagccttac aagcggttga ttttcttcag aaaaatgcaa
    19381 atccgctaac ccaaaacgtt ttgccatttc actataataa tctttacggc ttgggtgctg
    19441 ttcggcacat aaatgaaaaa tacgctgccc gttcggctgc tccaacagca atgaaatggc
    19501 tgcaatacag tcctctaaat gcactaaatt gaccggctgc cctgctccac tcaagttctg
    19561 ttttcccgcc aaataaaaaa ccgggtgccg ttttttgccc accaagccag ctaaacgcaa
    19621 aatatcacaa tgaattggct ggctcagcaa ccaattttcc atttttacta ataggttagc
    19681 agaatcaacc actgcatttt catcaaaaac gcccgatttc attggcaaaa ccgaagtcga
    19741 gctggtgaaa accagatgtt tcaagccctt ttctattgca agcgaaacta atgtttgaat
    19801 accgcaaccg taattttcag ggcgaatttg gctaaggggc aaggcaatca ctaacgcatc
    19861 agcagcaagt aattgctcga tttcgcttgt cactgctaac gcatttaaat ctagaggata
    19921 acattctatc gctaattctc cgaccgttcg cttagtacct gcaacacgcc agcccttatt
    19981 ttgtaatgct tcagctaaag gtagtccgag ccagccaagc cctaaaatgg taattttttt
    20041 catcatttac gtttctcaag attaaaattg gaatgagggg aaattcaggt acaaaaaaag
    20101 cacctcgtgg tgctatctat tttatttcta gattggagcg ggaaacgagg ctcgaactcg
    20161 cgaccccgac cttggcaagg tcgtgctcta ccaactgagc tattcccgca aattttaaat
    20221 tgttgatttt ctaaatggtg cccgaggcca gacttgaact ggcacgc
//
LOCUS       DANLZC010000029        20235 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.029, whole genome shotgun sequence.
ACCESSION   DANLZC010000029 DANLZC010000000
VERSION     DANLZC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20235
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.029"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..177)
                     /locus_tag="PXE44_000846"
     CDS             complement(<1..177)
                     /locus_tag="PXE44_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5588375.1"
                     /translation="MKVHEKIKLLRENNHWTQDEMAQKLSMTTKGYAKIERGETISNL
                     PKIEQIAEVFNMDIC"
     gene            312..494
                     /locus_tag="PXE44_000847"
     CDS             312..494
                     /locus_tag="PXE44_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588376.1"
                     /translation="MNNQYNPLYGMLGILASAASVILPLYAAYKDYQFGENMLSLMDI
                     IIFPLGIVRGLMYLFG"
     gene            498..827
                     /locus_tag="PXE44_000848"
     CDS             498..827
                     /locus_tag="PXE44_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588377.1"
                     /translation="MKKYLFFAFMTLSNVAYPNLDEWYSASDKERREFIAKHFAEETV
                     RSDRNTPYDYAMCTGRMFAKQLTRDEVKDYPLANTKPQYLTRDRRIEIMSNLPREILQ
                     NCAKYIQ"
     gene            complement(863..1615)
                     /locus_tag="PXE44_000849"
     CDS             complement(863..1615)
                     /locus_tag="PXE44_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL5588378.1"
                     /translation="MAYKPPFTVTTKMLNLVVEISHKLGTLQLDFERNLHLRKNNRLR
                     SIQASLAIEANSLTIEQVTAIIKGKKVLGHPKDIREVKNAYDAYDEMMHYNPYSIKDF
                     LKAHKFLTADLVNDAGAFRSKDVGIYNFSGQLVHMGARPQFVENLINELFSWAKQDDT
                     PALIKSSVMHYEIEMIHPFSDGNGRMGRLWQNVLLAQWNPLFAWLPIETMVYENQQRY
                     YQMLSLADSQNDSTGFIEFMLEMILETLLKLK"
     gene            complement(1654..2901)
                     /gene="clpX"
                     /locus_tag="PXE44_000850"
     CDS             complement(1654..2901)
                     /gene="clpX"
                     /locus_tag="PXE44_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDL5588379.1"
                     /translation="MAFEKEPHCSFCGKKRTEVDQLVEGTEGYICNECIEESYALLHG
                     EEEPLIDENQDDEGSFFTNVPTPHELHAHLDDYVIGQEHAKKVLSVAVYNHYKRLRSA
                     LSNHQATNGVELGKSNILLIGPTGSGKTLLAETLARRLNVPFAVADATTLTQAGYVGE
                     DVENVIQKLLMKCDFEAEQAERGIVFIDEIDKITRKSESASITRDVSGEGVQQALLKL
                     LEGTVANINPQGGRKHPKGETIPVDTSKILFICGGAFAGLDKIVEARTNTQGGIGFAA
                     ALKKDKDRQDLTELFKQVEPEDLVKFGLIPELIGRLPVVTPLQELDEEALIQILTEPK
                     NAIIKQYQALFEMEGVELHFTKDALISIAQKAISRKTGARGLRSIVENLLLDTMYDLP
                     TLNAKKVTIGKGCVENGDKPKVE"
     gene            complement(2901..3494)
                     /gene="clpP"
                     /locus_tag="PXE44_000851"
     CDS             complement(2901..3494)
                     /gene="clpP"
                     /locus_tag="PXE44_000851"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL5588380.1"
                     /translation="MALVPMVVEQTSKGERSYDIYSRLLKERIIFLNGQVEDNMANLI
                     VAQLLFLEAEDPEKDIYLYINSPGGVVTAGLAIYDTMNFIKPDVATLCTGQAASMGAF
                     LLSAGAKGKRFALPHARIMIHQPLGGARGQATDIQIQAEEILRLKATLTRRMAEHSGQ
                     SYEKVLADTERDNFMSAEEAAEYGLIDKVLTSRSEVA"
     gene            3606..3989
                     /locus_tag="PXE44_000852"
     CDS             3606..3989
                     /locus_tag="PXE44_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HDL5588381.1"
                     /translation="MTLTEIYQKFERCKSWEERYRLLIQLSRLLPKPSEQELAQIAEI
                     EGCESRLWFEFQAEPRKVVAYSDARLMQGILFIVSVALTEKTAEELKGFDLKTMFDEL
                     KISQNLTSTRLNGLGQLQKHIMMNL"
     gene            complement(4093..4169)
                     /locus_tag="PXE44_000853"
     tRNA            complement(4093..4169)
                     /locus_tag="PXE44_000853"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4133..4135),aa:Asp,seq:gtc)
     gene            complement(4258..5013)
                     /locus_tag="PXE44_000854"
     CDS             complement(4258..5013)
                     /locus_tag="PXE44_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5588382.1"
                     /translation="MKKSIYDTPIFFERYQQLRQNPISMNEVVEKPTMFSLLPDLTGK
                     KVLDLGCGTGVHLAHYLTLGAEKVVGLDLSELMLQQAESDLSAHWQKGNDFSLYCLPM
                     ERLNEISEGDFDVVTSSFAFHYIADFSELLAKISQKMTACGTLIFSQEHPIVTCYREG
                     YRWEKNQEKQQVAYRLNYYREEGERDRSWFQQPFKTYHRTMATIVNNLIQGGFEIVQM
                     EEPMLAGQPRWHNEFKDLQHRPPLLFIKAIKQK"
     gene            complement(5073..6068)
                     /gene="trpD"
                     /locus_tag="PXE44_000855"
     CDS             complement(5073..6068)
                     /gene="trpD"
                     /locus_tag="PXE44_000855"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDL5588383.1"
                     /translation="MQALLDKLFANQALAQAESHRFFSEVIKGNVSNEQLAAALIALK
                     LRGETVDEIAGAAKAALENANPFSTPDYPFADIVGTGGDGHNTINISTTSAIVAATMG
                     YKIAKHGNRSVSSKTGSSDVLSHLGINVDVSPETARLALDETNICFLFAPTYHSGFKY
                     AAPVRQALKTRTIFNILGPLVNPAHAKRQLLGVYSPELVKVYAETVKALGYEHTIVVY
                     GAGLDEVAVHGETQVAEIQNGQIDYYTLTPEDFGIERHSLEDLKGGEPAENAEKVTAI
                     LQGKGKLAHIDAVAANVALLMKTFGEDDLKANVQKVKETLESGKALETLRKLSTY"
     gene            complement(6086..6472)
                     /locus_tag="PXE44_000856"
     CDS             complement(6086..6472)
                     /locus_tag="PXE44_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HDL5588384.1"
                     /translation="MISVYGLKQTLADRRALIADVIFDCMQMSLGVPKQRHALRFDLL
                     DAENFYPPINRSQDFIVIEINLMQGRSEQLKKRLIKGLFSALHTKVGIVPMDVEITIK
                     EQPEHCWGFRGMTGNEATDLEYQAHR"
     gene            complement(6465..7055)
                     /locus_tag="PXE44_000857"
     CDS             complement(6465..7055)
                     /locus_tag="PXE44_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDL5588385.1"
                     /translation="MANILFIDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLAKA
                     ETTENCLVALSPGPGNPAEAGNLLEIIRRLKDKVPMIGICLGHQALIEAFGGKVVHTG
                     TVLHGKVSRIEHDNQAMFSGINNPMLVARYHSLMGDELPAEFIVNAKFEHIVMAIRHK
                     TLPICGFQFHPESILTVQGTKLLKQSVEWLLGEKND"
     gene            complement(7065..8621)
                     /gene="trpE"
                     /locus_tag="PXE44_000858"
     CDS             complement(7065..8621)
                     /gene="trpE"
                     /locus_tag="PXE44_000858"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDL5588386.1"
                     /translation="MPQLTITQTELPYFADTTRVYYALCGERPHTLLLDSAEIQSKNS
                     VKSLLFAKSAVHIFCEDQTVTFHALTPNGEAVLPLIANALSLLNPAPTCKFLDKNDRL
                     QAIFPTISNQLDEDSKLKTTTVFDGLRTVSQLFADSTSPIYLGGLFSYDLVANFIPME
                     NISLQDDGLSCPDYTFYLAEQLVTIDHQTETAVLNTFCFEPNEQTNLNQQAVEFTQKL
                     ANLSHIELPLANQTATSEATANIEDEDFKAIIRKLKHHINIGDVFQIVPSRRFSLPCP
                     NTLASYKQLKINNPSPYMFYMQGENFTLFGASPESALKYSQANRQLEIYPIAGSRPRG
                     FDEQGNIDPELDSRLELELRLDQKELAEHLMLVDLARNDVARVSESGTRRVVDLMQID
                     RYSQIMHLVSRVIGTLRKDLDALHAYQACMNMGTLTGAPKIKAMQLIYQVEQQKRHSY
                     GGAVGYLTSDGNLDTCIVIRSAFVQNGIAYVQAGCGEVLDSDPQLEADETRHKARAVL
                     KAIGQVNAKA"
     gene            8763..9380
                     /locus_tag="PXE44_000859"
     CDS             8763..9380
                     /locus_tag="PXE44_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL5588387.1"
                     /translation="MTQFFYIHPDNPQQRLINQAVEIIRNGGVIVYPTDSGYALGCAM
                     GDKHAMDRIVAIRKLPENHNFTLVCSDLSELSIYALVNNQAYRLIKNNTPNPYTFILP
                     ATKELPRRLMTKRKTVGIRVPDNAIALALISALGEPILSCSLMLPETEMTESDPDEIR
                     DHLQNRVDLIIHGGYLGQQPTTVVDLTDDVPVIIREGSGDPTSFN"
     gene            9526..10593
                     /gene="rluB"
                     /locus_tag="PXE44_000860"
     CDS             9526..10593
                     /gene="rluB"
                     /locus_tag="PXE44_000860"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDL5588388.1"
                     /translation="MTTFQKKSTATFRADKRGESRPSFKKEEQKSERKFGKTSEKPPR
                     PTSKPLTQRIAKKVEKTTASEVASQEKKAKVVGEKLQKILARAGQGSRRELEEIIAAG
                     RVSVDGKMATLGDRVQVSSSTKIRIDGHLVNLMPAQKEICRVLMYYKPEGELCTRSDP
                     EGRSTVFDRLPRLSGARWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVR
                     VFGNIDDAMLQRLRKGVQLEDGPANFKQIKVVGGTGLNQWFDVTLTEGRNREVRRLWE
                     SQGVEVSRLIRIRYGNIKLDKGLPRGGWEEMGLEQVNYLRELVGLPPETETKVEVGVN
                     RRRTNIRQIRKAVKQHQKYRG"
     gene            10659..12431
                     /locus_tag="PXE44_000861"
     CDS             10659..12431
                     /locus_tag="PXE44_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDL5588389.1"
                     /translation="MSWDIVATLAVTGIAVFLFATEKMRMDVVAILVLCSLVLLGQVD
                     TKSALSGFSNSATVTVTAMFVLAAGLQNSGALDSVGSLLAKVKSPWLFLLVLCGVNAA
                     VSPFVNNTAVVAVLIPIVIAAAQNIKMAPSKALIPLSFSSQMAGVCTLIGTSTNLLVN
                     AIAQKQGHSGFGMFEFAPLGIIFLVVGVAYLLVTSRFLLPESHLQLEDGEGFGKYVSE
                     LKVNKDSVLIGKSTFESGLNEEFNLFTIGVLRDGERLSTPSHQVLQKHDILLLRGESE
                     NLAKVREKYGLHHVVYGRRENDEDNLDEDLMVAEVMISPISRWIGGTIPILQQRWNKN
                     ATVLGIQRQSKVIRERLRTTAFKMGDILLLTLPKEDMETLRQDKDFIVLSSDLVKTEE
                     SWKSKFALGVMVLVVATAALGWVPISVSALIGAVAMCVAGCLTAEEAYQSIDWKIILV
                     LAGLLPLGEAMANSGAAQFIVDNTLGKVGEFGPLVVLAVLYLLTMVLTEFMSNAGTAV
                     LLTPIAVSTAKMLGVDASPFIIAVMFAAATSFMTPVGYQTNTMVYGAGGYKFTDFIKI
                     GLPLNVIYWILGIVLIPIFFPFNP"
     gene            complement(12521..16672)
                     /locus_tag="PXE44_000862"
     CDS             complement(12521..16672)
                     /locus_tag="PXE44_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5588390.1"
                     /translation="MNKIYRIVWNHAKRTWIVASELSRSANKSNATNTSLTTNIIKLS
                     TLSLALSAGFASAATYSPTYGTIPDNNPQTSIAIGDGSKVNSTLVSTITGLEGNSGKV
                     STALGYNTTATADFSTALGGFANASFASTAVGGQATADKRSVAVGYNATAMGLREVFI
                     GDHAGLNHSNSTEYNIGIGYSASSNVTGNNTISIGNTAGDGTSGSHNIAIGTYANAKL
                     AGPTTNVTSDDNIAIGNSALANGVNNYKTTAATGETVIGKATAVGSHANATGIVSSAY
                     GAEANATAIHSTAIGAKSQANGDNSTAIGYEAKTSGHGATSLGYYANATANLTTAVGT
                     NAGATSDYASAFGREANASGGSATALGNRATASGAASVALGVSAKATNQRTIAIGESS
                     NASAFNATAIGRNATAEHTDSIALGSNSVTAIAIPTTNATVNGITYSDFAGTNPIATV
                     SIGAEGKERTITNVAAGRISLSSTDTINGSQLYLTQQAIGNVAATTANILGGGAAVTE
                     NGNITFPTYALVNGTPDADKEGKQGRYTTVSAALSALNTAVISPLTFAGDTGTNFERH
                     LGSTVKIKGGSTGILTENNIGVVADGNSTLTIKLAEKVNLGANGSLTTGDTVVNNTGI
                     TIANGVADKPVSLTKSGLDNGGNKIANVAAGDVDTDAVNVSQLKQAISKFATHYVSIS
                     DDGIQRANYDNSGSSGVNPMAIGVATSANGELATALGSEAEANGERTTAVGPRATADG
                     MNATSIGYNANANATNALAVDSAANANADTSTAIGTASTATATRATALGSKSEATGEN
                     STAVGYEASSIGADSLAAGYNANASGTQSTALGNSANAGGIWSTSVGRNANAAGSSAI
                     ALGNSANAAGVASIALGVSSQATTTAAVALGQNAKATHQGSVALGTNSETVATVATKS
                     ATLNGNTYTFAGTTPSSTVSIGSVGNERTLTNVAAGRILDSSTDAINGSQLYAAYTEI
                     DGLNTKVNELSNGALTFVDDAGTEIVRKLGTSLNVKGGADATILTDNNIGVVATDANT
                     LTVKLAKDIDLTPAGSVAVGNSKLNNNGLTINNGPSVTMTGVDAGKLKITNVADGDIS
                     PISADAVNGSQLYDTANTIATALGGNSSVNANGAVSAPSYTVVDGAPTNEVSKTVNNV
                     GSAITALNDAVTSPLTFAGDTGTPSQRKLGSTVTVKGGVSNESQLTDNNIGVISNGNG
                     SLTVKLAKDIKVNSVTAQTVTANIAVADTVKTGDTTIDTNGLTIVGGPSITKTGINAA
                     GTKVTNVKAGTEDTDAVNFSQLKATEKNINNKINNIDSKVNKVDKRLRAGIAGATATA
                     GLPQAYLPGKSMLATAGDTYRNEAAIAVGYSRISDNGKVIYKLTGNSNTRGDFGGSIG
                     MGYQW"
     gene            17063..17794
                     /locus_tag="PXE44_000863"
     CDS             17063..17794
                     /locus_tag="PXE44_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDL5588391.1"
                     /translation="MGCSSFSTKLKQEFFSGWTTFEASWLIVFLAIQIGIFIYQPDTW
                     IATIAAITGILCVVFVGKGKISNYLFGLISVSLYAYISYTFQLYGEMMLNLLVYVPVQ
                     FIGFYFWRKNMTSENTVNNAGVEEVIAKALTAKQWVIVAITTIIGTFLYIELLKYLGS
                     ALAILDGATVVISIVAQILMVLRYREQWALWIIVNIMTISLWTAMYFQNGETSLPLLV
                     MYVMYLCNSIYGYYNWIKLHRKHQQ"
     gene            complement(17862..19901)
                     /gene="malQ"
                     /locus_tag="PXE44_000864"
     CDS             complement(17862..19901)
                     /gene="malQ"
                     /locus_tag="PXE44_000864"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL5588392.1"
                     /translation="MSNKQNTAHYFYSPNIKEYASPSAFEKIERLLNTDNDLQKNNQI
                     LPLVKVITQPNAEQQAVEISLNFTNVAKWQIVLENGEVRNGEIKAEQSVLTLPADLPL
                     GYHQLSISTENDEESCSLIVTPPKAFQPKPLEERKKLWGAFLQLYTLRSDNNWGIGDF
                     GDLKQFLANISDYGADFVGLNPIHALFPADPDAASPYSPSFRLWQNIIYIDVSAVEAF
                     QQSEQAQQWFHTKETQQQLAELRAKDFVDYSQVAQLKLKALQFAFEHFSQSSQQQSQT
                     AFDAFIEQAGEPLKIQATFDALHHWISSEFGEKGGWTNWAEEYQSYYSPKVAEFQISQ
                     ATQIRFYMWLQFVANQQLAECNRFAQSLSMPIGFYRDLAVGVTQSGAETWADKALFVQ
                     EASIGAPPDMLAPNGQSWGLSPMHPHILQSRGYQPFIDLVRANMKHCGALRIDHILGF
                     ARMWWVANGESAKEGIYVRYPLEDLLSILALESQRHQCLVIAEALGIVPEGILDSLEQ
                     KGIFAYNVFYFEKAENAFKPLENYPYQAMTTLSTHDLPTIQGYWKGYDFELGEKFDTY
                     PSKAVLRKLKEERSYDRLNIRNAVEKVIALEPNEVGVTIKFTHQLQRYVAHTHSALFG
                     TQPEDWLNMLEPVNIPGTSTEYPNWRRKLSATTEQIFANKDIQQLLKDINEIRKG"
BASE COUNT         5588 a         3984 c         4271 g         6392 t
ORIGIN      
        1 gcaaatatcc atattaaata cttctgcgat ttgttctatt ttcggtaaat tagagatagt
       61 ttcaccacgt tctatttttg cataaccttt ggttgtcata cttaattttt gagccatttc
      121 atcttgtgtc caatgattat tttctcgcag taatttgatt ttttcgtgta ctttcatata
      181 acacctatag attgtgggag ttgggatata acttgatgtg gtaaaggata aattttgtgg
      241 attgtagcag tttattgagc aatttagaat agagtaaatt ttatcttaag ctcacaggag
      301 aagaaacatt catgaataat caatataatc cattatatgg aatgctaggt attttagcgt
      361 cagctgcatc agtaattttg cctctttatg cagcatataa agactatcaa tttggggaga
      421 atatgttgtc tcttatggat ataattattt tcccgttagg tattgttcgt ggacttatgt
      481 atttatttgg atagattatg aaaaaatatt tattttttgc ttttatgaca ttaagtaatg
      541 ttgcttatcc taatttagat gagtggtact cagcttcaga taaagagcga agagaattta
      601 tcgcaaaaca ttttgcagaa gaaacagtaa gaagtgatag aaatacaccg tatgattatg
      661 ctatgtgcac tggtagaatg tttgctaaac aactgactcg agatgaagtc aaagactacc
      721 ctttggcaaa cactaagccg caatatttaa cccgtgatag acggattgag attatgtcta
      781 atttacctcg tgaaatatta caaaattgtg ctaaatacat acagtaattt tatgagtaac
      841 accactttgg aattaagtgg tgttatttta attttaataa tgtctctaaa atcatctcca
      901 acataaactc aataaagccg gtagaatcat tttggctgtc tgcgagagaa agcatttgat
      961 aataacgttg ttgattttcg taaaccattg tttcaatcgg caaccaagcg aaaagtgggt
     1021 tccattgtgc taaaaggaca ttttgccata atcgacccat tctgccgttt ccatcactaa
     1081 aggggtgaat catttctatt tcatagtgca taaccgagct tttaatgagt gcaggtgtat
     1141 catcttgttt agcccaagag aacaactcat taatcaaatt ctctacgaat tgaggacgag
     1201 cccccatatg cacaagttgc ccactgaaat tataaatgcc gacatctttt gaacgaaaag
     1261 cccctgcgtc atttactaag tcagcagtaa gaaacttatg tgctttgaga aaatctttaa
     1321 tcgagtaagg attatagtgc atcatttcat cgtaagcgtc ataggcattt ttgacttcac
     1381 gaatatcttt ggggtgtccg agaacctttt tgcccttaat gattgcggta acttgctcaa
     1441 tcgttaatga atttgcctca atagctaaag aggcttgaat tgatctcaag cggttatttt
     1501 ttctaagatg taaattacgc tcaaaatcca attgtagcgt tcccaattta tgagaaattt
     1561 ctacaaccag attcagcatt ttagtggtaa ctgtaaaagg tggtttataa gccattttgc
     1621 aataatccta aaaaattaaa ccgcttgtca ttgttactcc accttcggct tatcgccgtt
     1681 ttcaacacag cctttgccga tggtgacttt tttcgcattg agggttggga gatcgtacat
     1741 tgtgtcaagt aataagttct cgacaataga acgtaaccca cgagccccgg ttttgcggct
     1801 aatcgctttt tgggcaattg aaattaatgc atctttggtg aagtgcaatt caacgccttc
     1861 catttcgaat aaggcttgat attgcttgat gatcgcattt ttcggctcgg ttaagatttg
     1921 aatcagagct tcttcgtcca attcttgcag cggagtcacc accggtaaac gcccgattaa
     1981 ttcaggaatt aagccgaatt tgactaaatc ttccggttca acttgtttga atagctcggt
     2041 taaatcttgg cgatctttat cttttttcaa tgccgctgca aaaccgatac caccttgagt
     2101 gttggtacgg gcttccacga ttttatctaa accggcaaaa gcaccgccgc aaataaagag
     2161 aattttggag gtgtccaccg gaatggtttc gcctttcggg tgtttgcgac cgccttgtgg
     2221 gttaatgttc gccaccgtgc cttcaagtag ttttaataag gcttgttgaa caccttcgcc
     2281 cgatacatca cgggtaatgg atgcactttc cgatttacgg gtgattttgt cgatttcatc
     2341 aataaaaaca atgcctcgtt ccgcttgttc ggcttcaaaa tcgcatttca ttaataattt
     2401 ttgaatcacg ttttcgacat cttctcccac atagcctgct tgggtaaggg tagtagcatc
     2461 agctaccgca aaaggtacgt ttaaacggcg ggcaagggtt tctgccaaca aggttttacc
     2521 gctaccggtc ggcccgatta ataaaatatt acttttacct aactctacgc cattagtggc
     2581 ttgatggttt gacaatgcac ttcttaagcg tttgtagtgg ttataaacgg ccaccgaaag
     2641 cacttttttc gcgtgttctt gaccgatgac ataatcatct aaatgagcgt gtaattcgtg
     2701 aggtgtcggc acattggtga aaaaactgcc ttcatcgtct tggttttcat caattaacgg
     2761 ctcttcttca ccgtgtaata aggcgtagga ttcctcgata cattcattac aaatatagcc
     2821 ttccgtgcct tcgactaatt gatcgacttc ggtacgtttt ttaccgcaaa aactacagtg
     2881 gggttctttt tcaaatgcca ttacgccacc tcactgcgag aagttaatac tttgtcgatt
     2941 aagccatact cagcggcttc ttccgcagac ataaaattat cacgctcggt atcggctaac
     3001 actttttcat agctctgacc gctgtgttct gccatacggc gggtaagggt agctttcaag
     3061 cgtaaaattt cttccgcttg aatttgaata tcggttgctt gacctctcgc tccacctaaa
     3121 ggttggtgaa tcataatacg agcgtgcggc agagcaaagc gtttaccttt tgccccggct
     3181 gaaagcaaaa atgcccccat tgaagccgct tgccctgtgc aaagggttgc tacatcgggt
     3241 ttgatgaagt tcattgtatc gtaaattgcc aagcctgctg ttaccacgcc acccggtgag
     3301 ttaatgtata aatagatgtc tttttccgga tcttcggctt ctaaaaacag aagttgtgcc
     3361 acaatcaggt ttgccatatt gtcttccacc tgaccgttta aaaaaataat acgttctttt
     3421 aataaacgtg aataaatgtc gtaagaacgt tcgccttttg aggtctgttc caccaccatt
     3481 gggactaatg ccattttgtt ttcctctgtc gtaatacaaa atctcgctat tctagcaaat
     3541 tttcagaaaa tcattaacaa caagcggtca gattgttaaa atcttttgca aatgatgagg
     3601 aaattatgac attaacagaa atttaccaaa aatttgaacg ttgtaaatcg tgggaagaaa
     3661 gataccgctt gttaattcag ctaagtcgct tgttaccaaa accaagtgag caagagttgg
     3721 cacaaatagc ggaaattgag ggctgtgaaa gccgtttatg gtttgaattt caagctgagc
     3781 cacgaaaagt agtggcatat agcgatgccc gtttaatgca ggggatttta tttattgtga
     3841 gtgtagcttt aactgaaaaa acggcagaag agctgaaagg ctttgactta aaaacgatgt
     3901 ttgatgagct aaaaatttca caaaatttaa ccagcacaag gttaaatggg ttgggtcagt
     3961 tacagaaaca tataatgatg aatttgtaat tcaccctcgc cccttgtggg agagggtgaa
     4021 taaaatccgc aggattttat tcgggagagg ggaatataaa aaacctctga tttctcagag
     4081 gcttaaattt gttggcggaa cggacgggac tcgaacccgc gaccccctgc gtgacaggca
     4141 ggtattctaa ccagctgaac taccgctcca tactggtgct aaaataatag tggtttcctc
     4201 aacattgagc aaatagtttt tgcaaaatag tgtttaattg gttgtaaaat aaccacttta
     4261 tttctgcttg atcgccttga taaacagcaa cggcggacgg tgctgtaaat ctttaaattc
     4321 attgtgccac cgaggttggc ctgccagcat tggttcttcc atttgcacaa tctcaaaccc
     4381 gccttgaata aggttattca caatggttgc cattgtgcgg tggtaggttt taaagggttg
     4441 ttgaaaccaa cttctgtccc gttcgccctc ttcccgataa tagttcaagc ggtaagcaac
     4501 ttgttgtttt tcttggtttt tctcccaacg atagccttct cggtaacagg tgacaatcgg
     4561 gtgttcttgc gagaaaatca gcgtgccaca agcagtcatt ttttgcgaaa tttttgcaag
     4621 tagttcggaa aaatcagcaa tataatgaaa cgcaaaagag ctggttacta catcgaaatc
     4681 gccctcgctg atctcattta atcgctccat cggcaggcag taaagggaaa aatcgttgcc
     4741 tttttgccaa tgagcagaaa gatcgctttc tgcctgttgt agcattaatt cagataaatc
     4801 caaccccacc actttttctg caccgagcgt taaataatgg gctaaatgaa ctcctgtgcc
     4861 acaacctaaa tccagcactt ttttgccggt taagtctggc agtagagaga acattgttgg
     4921 cttttccacc acttcattca tacttattgg attttgtcga agttgttggt aacgctcaaa
     4981 gaaaatcgga gtgtcgtaaa tgcttttttt cacttaatag cctttaaaca aatataaaat
     5041 aaggcgggtt aaaacccgcc actacgatat gattagtaag tcgataattt tcgcaaagtt
     5101 tctaacgctt ttccactctc aagcgtttct tttacttttt gcacattggc ttttaaatca
     5161 tcttctccaa aggttttcat taataatgcg acatttgctg ccaccgcatc aatatgagcc
     5221 agtttacctt ttccttgtaa aatagcggtt actttttcgg cattttctgc aggttcaccg
     5281 cctttgagat cttctaacga atggcgttca atgccgaaat cttccggcgt taaggtgtaa
     5341 tagtcgatct gtccgttttg aatttccgca acctgcgttt cgccgtgaac ggcaacttca
     5401 tctaaacctg caccgtatac cacaatagtg tgttcgtaac ctaaggcttt tacggtttca
     5461 gcatagactt tcactaactc aggggagtac acaccgagta attggcgttt agcgtgagcc
     5521 gggtttacca acggccctaa aatattgaaa atcgtgcggg tttttaaggc ttggcgaaca
     5581 ggggcggcat atttgaatcc gctgtggtag gttggggcaa acaaaaagca gatgttggtt
     5641 tcatctaaag caaggcgtgc cgtttcaggt gaaacgtcaa cattaatacc taaatggctt
     5701 aatacatcgc ttgagccggt tttacttgat acgctacggt tgccgtgttt ggcaatttta
     5761 tagcccattg tggcggcaac aatcgcactt gtggtagaaa tgttgatggt attatgccca
     5821 tcgccacctg tgccgacaat atccgcaaaa gggtaatcag gcgttgagaa cggattggca
     5881 ttttcaagtg cggcttttgc agcacctgca atttcatcaa cggtttcgcc acgcagtttt
     5941 agagcgatta aggcagcagc aagttgttca tttgatacat tgcctttaat aacttcacta
     6001 aaaaaacggt gagattccgc ttgggctaag gcttggttgg cgaataattt atctaaaagt
     6061 gcttgcatag tctcttcctt tttaattagc gatgtgcctg atattccaaa tcggttgcct
     6121 cgttgccggt cattccacga aagccccagc aatgctctgg ctgttcttta atggtgattt
     6181 caacgtccat tggcacaatg ccgactttgg tgtgtaaggc actaaatagt cctttaatta
     6241 agcgtttttt gagctgttcg ctccgccctt gcatcaggtt tatttcaatc acaataaaat
     6301 cttggctacg gttaattggg ggatagaaat tttccgcatc caataagtca aaacgcaaag
     6361 cgtggcgttg ttttggtacg ccaaggctca tttgcataca gtcaaaaatc acgtctgcaa
     6421 tcagggctct gcggtctgct aaggtttgtt ttaatccata tacactaatc atttttttct
     6481 cctaataacc attccaccga ctgcttaagt aattttgtgc cttgcacggt taaaatggat
     6541 tccgggtgga actggaaacc gcaaatcggt aaggttttgt ggcgaattgc catcacaata
     6601 tgttcaaatt tcgcattaac gataaattct gccggtaatt catcgcccat taatgagtga
     6661 taacgagcga caagcattgg attgttaatg cctgaaaaca tcgcttggtt atcgtgttca
     6721 atacgagaaa ctttgccgtg caatactgtg ccggtatgca ctactttgcc gccaaaggcc
     6781 tcaattagag cttgatgtcc taaacaaatg ccaatcatcg gtactttgtc tttcagacgg
     6841 cgaataattt ccaataaatt gcctgcctca gccggattac ccgggccggg ggaaagggca
     6901 accaaacaat tttcggtggt ttcagctttg gctaaaaaat cctcaagcgg ataatcattg
     6961 cggaaaatcg ttacctgatg ccctaattca cgaaattgat cgaccaagtt ataggtaaaa
     7021 gagtcgaaat tatcaataaa aagaatattt gccatagttt gtccttatgc ttttgcattc
     7081 acctgcccaa tcgcttttaa caccgcgcgt gctttatggc gtgtttcatc tgcctctaat
     7141 tgtggatcgg aatccagcac ctcaccacaa ccagcttgca cataggcaat gccattttga
     7201 acaaaggcgg aacggatcac gatacaggta tctaaattgc cgtctgaggt taaatagccc
     7261 accgcaccgc cgtagctatg gcgtttttgt tgctcgactt ggtagatcag ttgcattgcc
     7321 ttaattttcg gagcaccagt tagcgttccc atattcatac aagcttgata agcgtgcaga
     7381 gcgtcaagat cttttctaag tgtgccgatc acacgagaaa ctaagtgcat aatttgcgaa
     7441 tagcggtcaa tttgcattaa atcaaccaca cggcgagtgc cggattcgct cacacgagcc
     7501 acatcattgc gggcaagatc gactaacatt aaatgttcgg caagctcttt ttggtctaaa
     7561 cgcagttcta attccaaacg ggaatctaat tcaggatcga tattaccttg ttcatcaaaa
     7621 ccgcgtgggc gagagccggc aatcgggtaa atttccaact ggcggttggc ttgcgaatat
     7681 ttcaacgcac tttcaggcga cgccccaaac agagtgaaat tttcgccctg catatagaac
     7741 atatacgggc ttggattgtt gattttgagc tgtttatagc tggcaagagt attcgggcaa
     7801 ggcaagctga aacggcgaga gggcacaatt tggaacacat caccgatatt aatatggtgt
     7861 ttgagtttgc ggataatcgc tttgaaatct tcatcttcaa tattggcggt tgcctcactc
     7921 gttgcggttt ggttcgctag aggcagctca atatgtgaca gatttgcaag tttttgcgta
     7981 aattcgaccg cttgttggtt caaattggtt tgttcattcg gttcaaaaca gaaggtgttt
     8041 agcaccgcag tttcggtttg gtggtcgatt gtaaccagtt gctctgctaa atagaaagtg
     8101 taatctgggc aacttaagcc atcatcttgc aggctgatat tttccatcgg aataaaattt
     8161 gccaccaaat cgtagctaaa taatccgccc aaatagatag gcgatgtgga atcagcaaac
     8221 agttggctca ccgttcttag cccgtcaaac acggtggtgg ttttgagctt gctgtcttca
     8281 tctaactggt tgctgatggt tgggaaaatc gcttgcaagc ggtcgttttt gtctaaaaat
     8341 ttgcaagtcg gagcagggtt taacaggctc aatgcgtttg caatcaaagg taaaaccgct
     8401 tctccgtttg gggttaaagc gtggaaagtc acggtttgat cttcacagaa aatatgcact
     8461 gccgatttcg caaacagcaa actttttaca ctgtttttac tttgaatttc cgctgaatct
     8521 aataatagcg tatgtgggcg ttcgccgcat aatgcgtaat aaacacgggt cgtatcggca
     8581 aaatagggta attctgtttg agtaatcgtt aattgaggca tagcaaattc ctattcaatt
     8641 tttcgtacta ttaaactagt acattaaaaa gaagtcaagc aattattcca gataattatg
     8701 cgagcttggg cgaacttggt gtataattag cccaattttt agaaagtcga aggcttgaga
     8761 ctatgacaca atttttttat atccaccccg acaacccaca gcaacgctta attaaccaag
     8821 cggtagagat tattcgaaac ggtggcgtaa ttgtttaccc taccgattca ggctatgctc
     8881 tcggttgtgc aatgggcgat aagcacgcaa tggatagaat tgtagcaatt cgtaaattgc
     8941 cggaaaatca caattttacc ttagtttgta gtgatttatc cgagctttct atctacgcgt
     9001 tggtcaataa tcaagcctat cgcttaatta agaataatac cccgaatcct tatactttta
     9061 ttctgccggc aactaaggaa cttcctcgca ggctgatgac aaagcgtaaa acagtgggga
     9121 ttcgtgtgcc ggataatgcg atcgcattgg cgttaatttc cgccttaggt gagccgattc
     9181 tttcttgctc gttaatgttg ccggagacgg aaatgaccga gtcagaccct gatgaaattc
     9241 gggatcattt gcaaaatcgg gtggatttaa ttatccacgg tggttattta gggcagcagc
     9301 ctacaacggt agttgactta accgatgacg tgccggttat tattcgagaa ggatcgggcg
     9361 atccaacatc gtttaattaa attgatgttg tatgattaat atatagaatt atgcgtaggg
     9421 gggctgtgcc agtccgtatt aatatcgtta tttggacagg cacaggacct gtccctacaa
     9481 tgaaaatcaa attcagacgc ttgtgaaagc gacaaaagga accttatgac aacttttcaa
     9541 aaaaaatcaa ctgcgacttt ccgtgcagac aaacgtggtg aaagccgtcc ctcatttaaa
     9601 aaagaagagc aaaaatctga acgcaaattt ggtaaaacgt cagaaaaacc accacgtcca
     9661 acttctaagc cattaacgca aagaattgca aaaaaagtgg aaaaaacgac cgctagtgaa
     9721 gtggcatctc aagagaaaaa agccaaagta gtaggtgaaa agctacaaaa aatcttagcg
     9781 cgagcagggc aaggttctcg ccgtgaatta gaagagatta tcgctgctgg acgagtgagc
     9841 gtggacggta aaatggctac tcttggtgat cgtgtgcagg ttagctcatc gactaaaatc
     9901 cgcattgacg gacatttagt taatttaatg ccggcacaaa aagagatctg ccgtgtatta
     9961 atgtattaca aaccggaagg ggaactttgc acccgttccg atccggaagg acgatccacc
    10021 gtatttgacc gcttgcctcg tttaagtggc gctcgttgga ttgcggtcgg gcggttagat
    10081 attaacacct caggcttatt attgttcact accgatggcg aattagccaa ccgtttaatg
    10141 cacccaagcc gtgaagtcga acgtgaatat tcggtgcgtg tgttcggcaa cattgacgat
    10201 gcgatgttac aacgcttacg caaaggcgtt cagttggaag acggaccggc aaactttaaa
    10261 caaattaagg ttgtgggtgg cacaggcttg aaccaatggt ttgatgtgac tttaaccgaa
    10321 gggcgtaacc gtgaggttcg ccgcctgtgg gaatcgcaag gggtagaagt cagccgtttg
    10381 attcgtatcc gttacggcaa tatcaaatta gataaaggct tacctcgtgg cggctgggaa
    10441 gaaatgggct tagaacaagt caattacttg cgtgagctgg tagggttacc gccggagact
    10501 gaaactaaag tggaagtggg ggtaaaccgc cgccgaacca atatccgtca aattcgtaaa
    10561 gcggtaaaac aacatcaaaa atatcgtggc taaacaccat ctttggggct gcattagttc
    10621 tgctaatgca gctattttct ttcaaaaaag gagcatttat gtcttgggat attgttgcaa
    10681 cattggcggt cactgggatt gccgtatttt tatttgcaac tgaaaaaatg cgaatggacg
    10741 tagttgcgat tttagtgctg tgttcattag tattattagg gcaggttgat accaaatctg
    10801 ctctgagtgg attttcaaac tcagctactg taaccgtaac tgcaatgttt gtattagctg
    10861 ctggtttaca aaatagtggt gctttagata gtgtaggcag tttacttgct aaggtaaaat
    10921 ctccttggtt attcttgctt gtactctgtg gcgtaaatgc tgctgtttct ccttttgtga
    10981 ataatacggc agtggtcgca gtattaattc cgattgtgat tgccgcagca cagaatataa
    11041 aaatggctcc ttccaaagcc cttattccgt tatcattttc ttctcaaatg gcaggtgttt
    11101 gtacactgat cggcacttcc actaatctat tagttaatgc aatcgctcaa aagcaagggc
    11161 attccggttt cggtatgttt gaatttgccc ctttggggat tattttttta gtggttgggg
    11221 ttgcctattt attagtgacc agccgtttct tattacccga atcacatttg caacttgaag
    11281 atggtgaggg gtttggaaaa tatgtttctg aacttaaagt aaataaagac tctgtattaa
    11341 ttggcaaaag tacatttgaa tcaggattaa atgaagaatt taatctattt actattggtg
    11401 ttttgcgtga tggggaaaga ttatcgacac cgagtcatca agtattgcaa aaacatgata
    11461 ttttattgtt gcggggtgaa tcggaaaatt tagcaaaagt acgagaaaaa tatggattgc
    11521 accacgttgt gtatggacgc cgtgagaatg atgaagataa tctggatgaa gacttgatgg
    11581 tggctgaagt gatgatttct cctatttctc gttggatagg agggactatt cctattttac
    11641 aacagcgttg gaataaaaat gcaacggtgt taggcattca acgccaaagt aaagtaattc
    11701 gtgagcgttt acgcaccact gcttttaaaa tgggggatat tttattactc acattaccaa
    11761 aagaggatat ggagacgtta cgccaagata aagactttat tgtgttatct tctgatttag
    11821 tcaaaacgga ggaatcgtgg aaaagtaaat ttgctttagg ggtaatggtt ttggtggtgg
    11881 caacagcagc cttaggctgg gttcctattt cagtgagtgc cttaattggt gcagttgcga
    11941 tgtgtgttgc tggttgttta acggctgaag aagcctatca atctattgac tggaaaatca
    12001 ttttagtctt agctggatta ctgccattag gtgaagcgat ggcaaatagt ggggcggcgc
    12061 aatttattgt tgataacacg cttggtaaag taggggagtt cgggccttta gtggtgttag
    12121 ctgtattata tttactgaca atggtgttaa ccgaatttat gagtaatgca gggactgccg
    12181 tgttgcttac gcctattgcg gtttctaccg ctaaaatgtt aggggtagat gcttccccat
    12241 tcattattgc cgtaatgttt gcggctgcga ccagctttat gacccctgtt ggctatcaaa
    12301 ccaacacgat ggtatacggt gcaggtggct ataaattcac agattttatt aaaattggtt
    12361 taccgctaaa tgtaatctat tggattttag ggatagtatt aattccgatt ttcttccctt
    12421 ttaatccata attgatagat aattatagaa aaaaggttta cagatcagaa actctgtaaa
    12481 ccttttttat tcagcaataa atcaattttc tattggaaaa ttaccattga taacccatgc
    12541 caatactacc accaaagtcg cctctggtat tgctgttacc agttaattta tagataacct
    12601 tcccgttatc tgaaatccga gaataaccta ctgcgattgc agcttcatta cggtatgtat
    12661 ctccagccgt tgctaacata cttttacctg gtagatatgc ttgcggtaaa cctgcggttg
    12721 cagttgcacc agcaatacca gcgcgtaaac gtttatcaac tttgtttact tttgaatcaa
    12781 tattattgat cttattattg atattttttt ctgttgcttt taattggcta aagttaactg
    12841 catcagtatc ttcagttcct gcttttacgt tagtcacttt tgttcctgct gcattaatac
    12901 ctgttttagt tatactcggt ccccctacaa tagttaatcc attagtatca atggttgtat
    12961 caccagtctt aacagtatcg gctacagcaa tgttagctgt tactgtttga gctgtgacag
    13021 aattaacttt aatatcttta gctaatttaa ccgttaatga accattacca tttgaaataa
    13081 ccccgatatt attatcagtt agttgtgatt cgttagatac ccctcctttt actgtaacag
    13141 tagaacctaa tttacgttga gatggcgtgc cggtatcacc tgcaaatgtt agtgggctgg
    13201 ttactgcatc atttaacgca gtaattgcag agcctacatt attgaccgtt ttactaactt
    13261 catttgtagg agcgccatca accacagtat agcttggggc tgaaacagca ccatttgcat
    13321 ttacacttga attaccacct aaagcggtag caatagtatt tgccgtatcg taaagttggc
    13381 taccattaac tgcatctgct gagataggtg aaatatcacc atcagctaca ttagttattt
    13441 ttaatttgcc agcatcgaca cctgtcattg tgacacttgg gccattatta atggttaagc
    13501 cgttgttatt tagttttgaa ttaccaacag ctacggagcc ggcaggagtt aaatcaatgt
    13561 ctttcgctaa tttaactgtt aatgtgttag catctgttgc cactacaccg atattattat
    13621 cagtcaatat ggttgcatcg gccccacctt taacattcag agaagtacct aatttacgaa
    13681 caatttcagt accagcatca tctacaaaag taagtgctcc attacttaac tcattcactt
    13741 tcgtgtttaa accatcaatt tcggtataag cagcataaag ttgtgaaccg ttgatagcat
    13801 ccgttgatga gtccaaaata cgacctgctg caacattcgt taaagtacgt tcattgccta
    13861 cagaaccaat acttacagtt gagcttggcg tagtacctgc aaatgtgtat gtattgccat
    13921 ttagcgttgc actttttgtt gcaactgtgg ctactgtttc agagttagta cctaatgcaa
    13981 cagaaccttg gtgtgttgct tttgcatttt ggccaagagc cactgcagca gttgttgttg
    14041 cttgtgatga aacacctaat gcaatagaag ctacaccggc tgcatttgca ctattaccta
    14101 aagcaatcgc agagctacct gcagcattgg catttctacc cacagaagtt gaccatattc
    14161 cacctgcatt agcagagtta cctaaagcgg ttgactgtgt acccgaagca tttgcgttat
    14221 agcctgcagc aagagaatct gcaccgattg aacttgcttc ataacctaca gcggttgaat
    14281 tttcaccggt agcttcagat ttagagccta atgcagttgc acgggtagct gtcgctgttg
    14341 aagctgtacc aatagctgtt gaggtatcag catttgcatt ggctgcagaa tcaaccgcga
    14401 gggcatttgt tgcatttgca ttcgcattgt agcctataga agtggcattc ataccatcag
    14461 ctgttgcgcg aggaccaact gcagttgtac gttcaccgtt tgcttctgct tcagagccta
    14521 atgcggttgc cagctcacca ttagcacttg ttgcaacgcc gatagccatt ggattaacac
    14581 cgctagagcc tgaattatca tagttagcac gttgaatgcc atcatctgaa atactaacat
    14641 aatgagttgc aaatttgctg atagcttgtt tgagttgact gacattgacc gcatcagtat
    14701 ctacatcgcc tgcggcaaca ttggcgattt tattaccgcc attatctaag ccagatttag
    14761 taagacttac cggtttgtct gcaacgccgt tagcgatggt gatacctgta ttgttaacaa
    14821 cggtatcacc tgttgttaat gaaccattag cgcctaagtt aactttttct gctaatttta
    14881 tggttaaagt actattgcca tcagctacaa caccaatatt attctcggtt aatatgcctg
    14941 tagatccgcc tttgatcttg actgttgaac ctagatggcg ctcaaaatta gttcctgtat
    15001 caccggcaaa ggttaacgga ctgatcaccg ctgtgtttaa tgcgcttagt gctgcagaaa
    15061 cagtagtgta tctcccttgt ttgccttctt tatctgcatc tggtgtgcca ttcactaaag
    15121 cgtaggttgg gaaagtgata tttccatttt cagtaacagc tgccccacca cctaagatat
    15181 ttgcggttgt tgcagcaaca ttacctatag cttgttgtgt taaatataat tgtgaaccgt
    15241 ttatggtatc tgtagagctt aaagatatac gtcctgcagc aacattggtg atagttctct
    15301 ctttgccttc cgccccaata cttactgtag caattggatt tgttccagca aagtcactat
    15361 atgtaatacc atttacagtt gcatttgtag ttggtatggc tatagctgta actgagttgc
    15421 tacctagcgc tatcgaatcg gtatgttcag cagtggcatt tcgacctata gcggttgcat
    15481 taaaggcgga agcatttgat gattctccga ttgcaattgt gcgttgattt gttgctttag
    15541 cgcttacccc tagtgcaaca gaagccgcgc ctgatgcagt agcacgattg cctaaagcgg
    15601 ttgcagaacc tccacttgca ttggcttcac gaccaaatgc agaagcataa tcgctggttg
    15661 cacccgcatt agtaccaaca gcagtagtta aatttgctgt tgcatttgca taataaccta
    15721 atgaggttgc accgtggcct gaagtcttcg cttcataacc aattgctgtt gagttatcgc
    15781 catttgcttg agattttgca ccaattgctg tggagtgaat agctgttgcg ttagcttcag
    15841 caccataggc ggagctaaca ataccagttg cattggcatg actacctaca gcagttgctt
    15901 ttcctattac cgtttctcct gttgctgctg tagttttgta attattaaca ccatttgcaa
    15961 gagcggaatt accgatggcg atattatcat cactggttac atttgtagta ggtcctgcta
    16021 gttttgcatt agcataagta ccaattgcaa tattgtggct accactagta ccgtctccag
    16081 cagtattacc aatagaaatc gtattgtttc ctgttacatt agagctagcc gaataaccaa
    16141 taccaatatt gtattctgtg ctgttagagt gatttaatcc ggcatgatcg ccaataaaaa
    16201 cttcacgtaa gcccattgcg gttgcattgt accctactgc tacggaacgt ttatcggcag
    16261 tggcttgccc accaacagct gttgaagcaa aactagcgtt agcaaagcct cctaatgcag
    16321 ttgaaaaatc ggctgtcgcg gttgtgttat accctaatgc tgtagaaact ttaccgctat
    16381 ttccttctaa gcctgttatt gtgcttacta gtgttgagtt aactttagat ccatcaccaa
    16441 ttgcgataga tgtttgtggg ttattatctg gtatggtccc ataggttggt gaataggttg
    16501 cagcacttgc aaaacctgca gaaagcgcaa gacttagtgt gctgagttta ataatattag
    16561 tggttagtga tgtatttgtt gcattagatt tattagcact tcttgataat tcagaagcaa
    16621 caatccaagt tcgttttgca tggttccaaa caatgcgata aattttattc atagaactac
    16681 tcctgttctt attttttaat aattttaatg atttgagtat tgcctagcca aatatataca
    16741 ataggctgaa aaattatata ggtgaggttt tatggtgtaa atagtggctt tttaggaata
    16801 aatatagcaa ttttttgata taaatgtatt attttaaatt ttatgttttg tactaatatt
    16861 gaggtggagt gcagagtttt atttggtttt tggtatgttt tttagtttta aaaaacactg
    16921 atttagttaa atgccaatta aatataggtt tattaatata ataaatgttt cgtatattta
    16981 tcaaaaggta ctctgtattt tgatttctta atttaaataa cttagaattt gaaaagaata
    17041 cttcacttta tatggaggaa atatgggctg ttcatctttt tcaactaagt taaaacaaga
    17101 gttttttagt ggttggacta cttttgaggc aagttggttg attgtttttt tagcgattca
    17161 aatcggtatt tttatttatc aacccgatac ttggattgca actattgcgg caataacagg
    17221 tattttatgc gtggtatttg tcggtaaagg aaaaatcagt aactatctat tcggcttaat
    17281 ttccgtttcg ctttatgctt atatctctta tacctttcag ttatatggtg aaatgatgct
    17341 gaatttactt gtatatgtgc cggtgcagtt tatcggtttc tatttctggc gtaaaaatat
    17401 gaccagcgaa aacacggtaa ataatgctgg tgtggaagag gtaattgcta aagctttaac
    17461 cgcaaaacag tgggtgattg tcgccattac caccattatc ggcacatttc tttatattga
    17521 attgcttaaa tatttaggca gtgcgttagc tatattagat ggtgcaacag tggttatttc
    17581 gattgtggca caaattttga tggtacttcg ctaccgtgag caatgggcat tgtggattat
    17641 cgtgaatatt atgaccattt ctttatggac ggcaatgtac ttccaaaacg gcgaaaccag
    17701 cttgccgctg ttggtgatgt atgtgatgta tttatgtaac tctatttacg gttattacaa
    17761 ctggattaaa cttcacagaa aacatcagca atagagcgaa taaatacaaa ttacaagcgg
    17821 tcattttttg taaaaattta cctaaaaatg accgcttgtt attatccttt acgaatttcg
    17881 tttatatctt ttagcagttg ttgaatatct ttatttgcaa aaatctgctc ggtggttgcg
    17941 gataattttc ttctccagtt agggtattcg gtgctggttc ccggaatgtt caccggctct
    18001 aacatattga gccaatcttc gggctgtgta ccgaataatg cactgtgagt gtgagccaca
    18061 tagcgttgta attggtgggt aaattttatt gttactccca cttcatttgg ctcgagtgct
    18121 atcacttttt ccaccgcatt acggatattc aagcggtcgt acgaacgctc ttctttcagc
    18181 tttctcaata cggctttgct tgggtaagtg tcaaattttt cgcctagctc aaaatcatag
    18241 cctttccaat agccttgaat agtcggtaaa tcgtgggtgc ttagagttgt catcgcttgg
    18301 taaggataat tttccagcgg tttaaacgcg ttttctgcct tttcaaaata aaaaacattg
    18361 taggcaaaaa tgcccttttg ctcgagagag tccaaaatgc cttcaggcac aatgccaagt
    18421 gcctctgcaa tcactaaaca ttggtgacgt tggctttcta atgccaaaat agagagcaaa
    18481 tcttcgagcg gataacggac ataaatccct tcttttgccg attcaccgtt cgccacccac
    18541 cacatacggg cgaagccgag aatatggtcg attcgcaatg ccccacaatg tttcatatta
    18601 gcacgcacta aatcaataaa cggctgataa cctcggcttt gtagaatatg cgggtgcatt
    18661 ggcgaaagtc cccagctctg tccgttcggg gcaagcatat caggtggtgc accgattgac
    18721 gcctcttgca caaatagggc tttatccgcc caagtttccg ctccgctttg agtaacacca
    18781 acggctaaat cgcggtaaaa accaatcggc attgaaagtg attgagcaaa ccggttgcat
    18841 tctgctaatt gctggtttgc cacaaattgc aaccacatat aaaaacgtat ctgcgttgcc
    18901 tgcgatattt gaaattcagc tactttaggc gaatagtaac tttgatattc ctccgcccag
    18961 tttgtccagc cgcctttttc gccaaattca gatgaaatcc agtggtgcaa agcatcgaac
    19021 gttgcttgga tttttaatgg ctcgcctgct tgctcaataa aggcatcgaa cgctgtttgc
    19081 gattgttgtt gagatgactg tgaaaaatgc tcaaaagcaa actgcagagc ctttaatttc
    19141 agttgcgcta cttggctgta atcgacaaaa tctttggctc gcaattcggc aagttgctgt
    19201 tgagtttctt tggtgtgaaa ccattgttga gcctgttcag attgttgaaa ggcttctact
    19261 gctgaaacat caatataaat aatattttgc caaagtcgaa aggacgggct gtaagggctt
    19321 gccgcatcag gatctgccgg aaagagggcg tgaatagggt ttaagcccac aaaatctgca
    19381 ccataatcag aaatgttggc aaggaattgt ttgagatcgc caaaatcgcc aattccccag
    19441 ttgttatcgg aacgcaaagt ataaagctgt aaaaatgcac cccagagctt tttcctttct
    19501 tccaatggct taggctggaa agctttcggc ggtgttacaa tcagcgagca agattcttca
    19561 tcattttcag ttgaaataga aagctgatga tagcctaaag gcaaatcggc aggcagggtt
    19621 agaactgatt gctctgcctt aatttcaccg ttccgaacct caccattttc aagcacaatt
    19681 tgccactttg ctacatttgt aaaattcagc gaaatttcga ccgcttgttg ctcggcattt
    19741 ggctgagtaa tcactttaac aagcggtaga atttgattat ttttttgcaa atcgttatcg
    19801 gtatttaaaa gccgctctat tttctcaaaa gcagaggggc tggcatactc tttaatattt
    19861 ggggagtaaa aatagtgagc tgtattttgt ttgtttgaca ttatgacttt tttcctttac
    19921 tgtatttgtt aaaataacga tcttgtgaag attaatatga gtaagaagag taggcaacag
    19981 gtttgtcttt gtgatatgat gttaaaaaaa tttaatttaa ctctaaaggg ttatgtttta
    20041 ttattaaata taccattttt gtttttatta atatcctgtg ctgtgctttt ttctgatttg
    20101 cgtagctttg aggcctggaa ttcaaataca tttttcattt attttataac tctattatat
    20161 ttgctggtta gtttattttt atatcctttt gtagctcagg ctcttaggat taaacttaaa
    20221 aaaaagtatg agtta
//
LOCUS       DANLZC010000030        20155 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.030, whole genome shotgun sequence.
ACCESSION   DANLZC010000030 DANLZC010000000
VERSION     DANLZC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20155
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.030"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..564
                     /locus_tag="PXE44_000865"
     CDS             <1..564
                     /locus_tag="PXE44_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2572 family protein"
                     /protein_id="HDL5588393.1"
                     /translation="YREKYLSDQYNFLALYPQDKTQICQKFQKEQISQADLSDKQFRF
                     LQYQFACQFHSLFKDKKPTKEKYIHFTHLADYLDLSNVPNQEIYTIHSLAELPSSSER
                     EPKIVLAKNAINENLPQEFYGIVITDYLFDITGKKMYGTLYSSYDNAREERNLTFKKE
                     VLAELEARYSAWRYLPYSEHLMGAVDD"
     gene            557..850
                     /locus_tag="PXE44_000866"
     CDS             557..850
                     /locus_tag="PXE44_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5374 domain-containing protein"
                     /protein_id="HDL5588394.1"
                     /translation="MIKYKAETALSLLVAITLFAIVVLSFSQWQSAQNHRLNQHFQQQ
                     QAVMILENQLALKLAGLDCESSITQNQIRYQIQCSGNRLTIRFPLGKIELNND"
     gene            899..4189
                     /gene="recC"
                     /locus_tag="PXE44_000867"
     CDS             899..4189
                     /gene="recC"
                     /locus_tag="PXE44_000867"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDL5588395.1"
                     /translation="MFTLYQSNQISSLAEMLVKIQQVNPLEDPFEPETILIQSQGMAQ
                     WLQMQIAELNGVMGNCDFLYPTTFLWQQYRLLFPELPKENIFERSSLVWRIMRLLPSC
                     LTLSEFAPLKRYLGDQPNKEQYQLKLYQLSAKIAELFDQYLVYRPHWLVSWEEGNLQA
                     IENEIKIATAAKPIDFDLIRADMKWQSHLWQQIVSDLQSESDEQVFTTSHRAYLQQNY
                     FAKLDDLTETEKAKLPKRIFIFGISSFSPLQLAVFKKLSEHCDIHLFFFNPSEKYWGD
                     SVEEKVWQKLALSHKISQDDLDNLLAEQRNKLLNLWGKQGRDFLAQLIEFEPNTMDVF
                     VEPEENSNLNKLKKQIFSLENDATITDLTADNSLQIHSCHSPMREVEVLHNQLLNLFE
                     QDSTLLPKDIIVMSPDINKYAPYIDAVFSRYNTGNRDPRYIPFSLSDQAFTQVDPIIH
                     SFLQLLAMKESPFNAEELFDLLEVNAIQTRFGFSTEDIHTLRVWVRNAGVRSGVEIEQ
                     PHWQNYNSWENGLNRLLLGSSLKEEQGIWENTLAFGDSYGLNAELVGRLASFIEALLA
                     WVNFLQTPQPLAAWQQAIKNLTADLYQENAENAASILLLNNVNDQIIEQISATHFSDD
                     LQIEVLALLFEQQLSQHSQQLNFLVGRVNFATLLPMRAIPFKVICLLGMNEADFPRQQ
                     QINSFDLMQYAPKKGDRAKRDDDRYLFLEALLSAQQMFYISYIGQSQKDGKEMLPSIL
                     VSQLLDAMNEALTPECKQQLSDEVEVLVRKQPLSVFSKKNFTHPRDHAYNAEWIIKPT
                     VAVQDFLSEPLTNESVASLELDELISFVQNPIRFFFLKVLGISFNTYDDTIDESEIFE
                     MSGLDNYRILNELLTINLAQQQRFFAEEQLKGNLPASYFAQIATQDLIGKITEMQAAL
                     SHYLASESRILSVNREVELERQTLTLNGNIANLYQNEVVLWRVGHLRDKDKIQTWIYH
                     LLLCAYFPEKTVKFYYREGEQVGVLSFETVSQIEALSQLSIYLNAYLDGLSQAQLVIY
                     DRIEEYLKGDGTNSEQVLIDLAEKDERDGSYLHRLLAQTKELDSQAIHQRTQSWFSLM
                     NQKIAKGD"
     gene            4325..7270
                     /gene="ptrA"
                     /locus_tag="PXE44_000868"
     CDS             4325..7270
                     /gene="ptrA"
                     /locus_tag="PXE44_000868"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDL5588396.1"
                     /translation="MKLSFLTQFVAAAFALNVMTYPAFAEVAKNSEKTTASQPQSQLG
                     FELIKQSINKSPNDKAIYQGIKLDNGMEVLLISDSQANKSLMSAIIPIGSMDDPISQQ
                     GLAHYLEHMILMGSKNYPETNSLDGFLTKNGGMNNASTASHRTAYYLQVNNEAFSEAV
                     TRLADTLAFPLLLETNAKKEVNAVNAEMVRAKSHDGHLLHSVNLATANPAHPATKFAV
                     GNKETLSDKPDSKLQTELEQFYKQHYSANLFKVVLYSNQSIEQMAKLAANTLGKMENK
                     HFSIPQTAEPRYRDEDKSVFIQYKPVKSNKMLVIGFDFPNDEMQFKHKTGKYIAYMLN
                     NNTDGTLSDYLIKQGLSDGGIEAVSSANIGRDRGSFDIYIELTDKGLSEQDQIISLVF
                     QQIEKIKQEGIQESYFSEVKESLKQDFQHLQVEKNMDFVEFIADQMLHFPLEHVIDQP
                     YLTETMDNEAIKAKLAAMNIDNARIMLVSDTAKTDKKTPYFEAGYSVAKITQEQKQKW
                     LDFSKNPQLNLPELNPYFTTDFTLNKVETGLTKPKLINDQAGNLIYTMPSVYFTNEPK
                     AKIGVGFNIRPRKDDLKSLTAASILGYMNNLAQTKLDFQSSVAGMNLSLAPAANGLAL
                     SAEGYTQNLAKLMLDSVKQFSRFEFNEDVLAQAKQRLHQELDRNEKENSLNQANAALS
                     RFASYPYFDVPKQREMIDSITLADIEQLRQRILQQSSGFKLLSVGNLSAEQVSALAED
                     LQAVIKNQKSANATGQYVDVSTSQRKINVIKSVPNEDNALLMAFMPNGYNELESLTRA
                     KLLRSIISRWYFDDLRTDKQLGYVVYATDNIIGKTAGIQFMVQSPNTTPAGILEHNER
                     FFTQSFERLKNLSDEEFGKYRDSLVEKLQYKPESLAEEFSEFGSDFSRNNPKFDRLQQ
                     VIELTKALTKADIVAFYQKAVIEKQGFVFVSQAVGTKTKAEDVAKFDGFEQIESIEKL
                     QREFEIKDY"
     gene            complement(7319..8965)
                     /locus_tag="PXE44_000869"
     CDS             complement(7319..8965)
                     /locus_tag="PXE44_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5588397.1"
                     /translation="MKFPTLTKISASVLAVLALAACDDKSNENKPTAAAAKPQTEKTF
                     VNCVSRSPTGFSPILMMDGLSYNASSQQIYNRLVEFVPGTTDIEPALAESWEISEDGL
                     TYTFKLRKGVKFHSNKDFTPSRELNADDVVFSFNRQLDKNHPYHTVSNGTYPYFNAMK
                     FPTLLKSVEKVDDSTVRITLTKRDASFLSSLGMDFTSIYSAEYADKMMKAGIPEAVDT
                     TPIGTGPFVFSGYVLDQASRYVANKEYWKGKPDIDRLIFEIVPDATTRYAKLQSGQCD
                     LMDFPNATDIEKMKNDPKINLLSQPGLNIAYVAFNTEKAPFDNVKVRQALNLAVDKKA
                     IIDVVYQGNGFAAKNPLPPTIWGYNDSLADSEYNVEKAKQLLAEAGFPNGFETELWVQ
                     PVVRASNPNPRRMSEIIQADWEKIGVKAKLVTYEWGDYIKRTKAGELTAGTYGWSGDN
                     GDPDNFLSPLFGTPNIGNSNYARFTNPELDALLEKALGSSDKAERTKLYEQAQVILRE
                     QAPWINVAHSINFAPTSKRVEGYKQSPFGYTYLYPVKIVD"
     gene            9041..11131
                     /locus_tag="PXE44_000870"
     CDS             9041..11131
                     /locus_tag="PXE44_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL5588398.1"
                     /translation="MIFANQSIELDTVNVIATRDPSKFADTPQKQTKDALLVKQATSV
                     AAALKALPNVDVQGGARAIVQKPSIRGLSDNRIVQVIDGVRQNFDLAHRGSYFVPMSL
                     IQEIEVIKGPSSSLWGSGALGGVVAMRTPNALDLLKNNDKFGAKIRQGYQTANNLSET
                     EASVFAANDRFDALLSGFYNNADNLRSGEGKELNNTGYKQAGGLVKFGWQINDTHRLE
                     LSHRHSQFKQTAPGNNEVENQFTDNDVSAQISAWHAANPGNPSNPVYLAKMLAFYGQL
                     HSSLGSVSYLSDQKITDKSTSLNYYFNPSNPYLSTQVTLYSNSTTENEHRKISGVEDQ
                     TKLSTRGVNIRNHSELSWFSLVYGVDYMLDKVATQRGRNNTDAQYRANPYDAKSKTTG
                     AYLIAHIPLFDEKVVFSPSVRYDRFDTSSETVKYKDSHWSPAAKLTWKATNWLDLTAK
                     YNEAFRAPSMQERFVSGSHFGTTVRGLPINNIFLTNPNLRPETAKNKEVTFNVHFDNV
                     ISQGDKFNFEATYFRNDVKDLINLQVFKANPTSPMEFIPTRSQYQNIANARLSGIELQ
                     TRYQTERLAVFANYGSTKGKDKDSGEALSNIAASKIGFGVNYAVVQDKFTVGANVTRY
                     QAQHRVPKNHGVTYQGYTLTDLHATYAPLKGEWKNLRLDVAVENLFDKKYQPAFSLME
                     GSGRNVKLSAAYSF"
     gene            11202..12890
                     /locus_tag="PXE44_000871"
     CDS             11202..12890
                     /locus_tag="PXE44_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL5588399.1"
                     /translation="MKFYPSLIASAVSFAVVLSGQAAVNVPDAGSLQRELDQRQAQQK
                     VKPSGEWFNQAAKAPYTATDGQELTFPLTQVQVFDRTNQEISTPELAPILANYVGKAV
                     SLTDLSNLANEITEFYRSHNYLVAKAILPPQEIEQGKVKILLFKGNVGEVRLQNQSAL
                     SDKFVARLANTTVNGSEFILKDELEKFALTMNDVPGVNTGLELSAGKQAGEANLLIKL
                     QNAERFSGYVSADNQGNKNTGRYRLATGIKANNLIGWGDELKVDLMSSNNANLKNARL
                     DYSSLIDGYATRLGLTANYLDYELGGNFKALQSQGHTHNLGAYVLHPTIRLPDFRLNT
                     KVSFNHQRLTDKQQAVNVAQKRQVNSLTVGINGSWNSLKDGTSYFSLSALFGNLANHT
                     NEQAHYVATDFQPKSRFTVYQYSLSHEQALPKSFAFNLGVNGQFADKTLDSSQKMLLG
                     GLYGVRGHQAGVASVDEGHLVQTELKHYLPLFSKSLLTSSVFYDYGWGKYYKHSGFLA
                     RGVNNSVKLQSIGAGLSLSEAGSYAINASVAKPLGKKIENADKHQFWFSAIKTF"
     gene            12902..15718
                     /locus_tag="PXE44_000872"
     CDS             12902..15718
                     /locus_tag="PXE44_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HDL5588400.1"
                     /translation="MFKRNAISLCILTACSGLAYANTADLPQGERVRVGTATIERTTD
                     KMTVNQSSNKVQIDWNSFDIGKNKEVEFKQPTEMAVAYNRVTGGNASQIQGKLTANGK
                     VYLANPNGVLITKDAEVNVGGLLVTTKDLEKISENGTKDKFSRKLAKEGKILNQGKIT
                     AKGERAFVVLVGDDVANEGEINAKNYYHKETKKVTECYDDSWSLVKQCYDFDKEIVKS
                     TSGEVHLSSGENVTFTLSDRYIDVALDDNTVNSIIKNDGAIIANGDITLTAKGRNQAL
                     DSLIINNGVLQANKVQNKNGTIILSAGETQLHEKSQIKGEKVSFAEASYYGPTEDIKV
                     VSKTGSKVTAPVIDFKGKTVNIQGEFGREDSTDLYNDELQILKTTVNIEVPNTEDIRI
                     ADTDNGGSGSFIQAGALASLLANNGRVNVQGKGFDVSGKLNVNAFKQTDSVLKFINQA
                     GINIHNADIHANGRLFFVTNLPNDTDFQSNIRITDSKINLGNGSMGFGRSSKRWEMHD
                     VVRRADTELRKKFNLDMKNVELNQVDDFVVAGGFQQVNLDNVKARGKTNFYLDSGNSR
                     MFVGEQIYEYGVTDIEKRTQLANLDQRRKRWNSDNYDETDLNWEYNQRFDGFAAKHNH
                     RAPIADTEINIANSDISLDNGFVHLMAEKINLDDSKINITFNQDNSLDESTQINRLGL
                     NGKVSMKNSHINVVGDEKDGISPARHYATMFLVGELLGEKSSIFLKSHQGAVLRTDSN
                     VTIAGKNSKDDLKITAITTGKKMGNEIIGPDGLPTSEDNDANASNTAFAIGYEPNATA
                     IVENASITALAPNGGLAYTGTSKTQIDAKDNATFTFFEKPRAGYDKLLGDIKGNPDKL
                     TPRDFARLYDNIKGIKASSLSEQQLGLADTAKKTSEHTLANSLDVENLVSVTVCDEQN
                     KCEERMLGNKNTNAKVIVGDIKFE"
     gene            15841..15972
                     /locus_tag="PXE44_000873"
                     /pseudo
     CDS             15841..15972
                     /locus_tag="PXE44_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(16027..16788)
                     /locus_tag="PXE44_000874"
     CDS             complement(16027..16788)
                     /locus_tag="PXE44_000874"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL5588401.1"
                     /translation="MKLNQAQIQNALICGQAVVFDEIDSTNEYLLTHHQRLENGTVCL
                     AEKQTAGRGRRGRTWYSPESENLYFSILWHYPMEQAADLPPLSLVVALIIAESLTVQN
                     VKDIQIKWPNDIYYQGKKMGGILLETQANRNGLDVVIGIGLNLGMAKVDENIVTQAWA
                     DLSQYHFNRNELVCRLAYELQKNLKIYPLVGFAHYAERWQSFDLFRHKAVKLITEAEE
                     IHGISQGINEQGELILKQGDNLRYFGIGEISLRAD"
     gene            complement(16800..17831)
                     /gene="murB"
                     /locus_tag="PXE44_000875"
     CDS             complement(16800..17831)
                     /gene="murB"
                     /locus_tag="PXE44_000875"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL5588402.1"
                     /translation="MMKHSLTPFHTFHLPAKATQIIEFTTVEQLLSEWQKAFNAQLPI
                     LILGQGSNVLFLEDFDGVVLVNKLKGITHHEDEHFHYLHVQGGENWHELVKWTLAHNI
                     AGIENLALIPGVASSAPIQNIGAYGVEFEQVCDFVEVLNLRSGELFKLSKSECEFGYR
                     ESVFKHQYRDEFAIISVGLKLAKAWKPVLSYGSLAQLNPETVTPIQIFDEVCAVRSSK
                     LPNPDEFGNAGSFFKNPVISNEQFAPIQAKFPAIPHYPQADGTVKLAAGWLIDQTGLK
                     DLQIGGAAVHTQQALVLINKENATGQDVLALAKTVRQRVKEKFGVEIQPEVRFIGKNG
                     EVDSEKITR"
     gene            complement(17935..18609)
                     /gene="crp"
                     /locus_tag="PXE44_000876"
     CDS             complement(17935..18609)
                     /gene="crp"
                     /locus_tag="PXE44_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010787261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDL5588403.1"
                     /translation="MQDMTLSTAPLENVTPPPSIHDPAVEWFLTHCHIHRYPAKYTLI
                     HAGEDAESLFYIVNGSVSVYIKDDETKEMLLTNLGTGEFVGELSLFEEKVQQRTAYVR
                     TKGPCEIAEISYKKFKQLVHLNPDILMYLSAQMARRLRQTSRQVGNLAFLDVAGRIAQ
                     TLLNLAKMPDAMTHPEGMQIKITRQEIGQMVGCSRETVGRIIKMLEDEGLISAHGKTI
                     VVYGAR"
     gene            complement(18671..18889)
                     /locus_tag="PXE44_000877"
     CDS             complement(18671..18889)
                     /locus_tag="PXE44_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDL5588404.1"
                     /translation="MIIPWQELEESTLHNILDSFILREGTDYGERELSLAEKRERLLT
                     QLKADKVVIVWSELHESLDIKDKKSFLG"
     gene            complement(18892..19497)
                     /gene="slmA"
                     /locus_tag="PXE44_000878"
     CDS             complement(18892..19497)
                     /gene="slmA"
                     /locus_tag="PXE44_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDL5588405.1"
                     /translation="MTEPKIKMPKKSVVERRQQVLEVLISLLNSEEGMQRITTERLAA
                     AVGVSEGALYRYFPSKTKMFEALIEKIEQTLTSYIHANKRQDSSANSVRAILYVILEF
                     ARKNPGVTRILTGHALMFEEELLKARVAKFFDNLELQFANILQLSKLRERKSFEDERA
                     LAGYLVNFCEGQFLRLVRSNFSYNQHQHFEKQWAFIKPLFD"
     gene            complement(19509..19964)
                     /gene="dut"
                     /locus_tag="PXE44_000879"
     CDS             complement(19509..19964)
                     /gene="dut"
                     /locus_tag="PXE44_000879"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL5588406.1"
                     /translation="MKQIDLKILDSRIGNEFPLPAYATEGSAGLDLRAMLNEPLTVKA
                     GETVLIPTGISIYIADPNLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGELKVSLWN
                     RSQADFTVNVGDRIAQLVFVPVVQASFNIVQEFEQTDRGEGGFGHSGKQ"
BASE COUNT         6040 a         4191 c         4386 g         5538 t
ORIGIN      
        1 tatagagaga aatacctttc cgatcaatat aattttttag cgttatatcc acaagataaa
       61 acgcaaatat gccaaaaatt tcaaaaagag cagatcagcc aagcggattt atccgataaa
      121 caattcaggt ttttacagta tcaatttgcc tgccaattcc atagtttatt taaagataaa
      181 aaaccgacta aagagaaata tattcacttt acccatttgg cggattattt agatttatca
      241 aatgtgccaa accaagagat ttatactatt cattctttag ctgaattacc tagtagcagt
      301 gaacgtgagc ctaaaattgt gcttgctaaa aacgcaatta atgaaaactt accgcaagag
      361 ttttacggca ttgtgataac cgactatctg tttgatatta ccggcaaaaa aatgtacggc
      421 acgctttatt cctcttatga taatgcccga gaggagcgaa atttaacgtt taaaaaagag
      481 gtactggcag agcttgaggc gaggtattcc gcttggcgat atttgccata ttccgaacat
      541 ttgatgggag cggttgatga ttaaatataa agcagaaacg gctttgtcgc ttttggttgc
      601 catcacgcta tttgcgattg tggtgttgag tttcagccag tggcaatcgg cacaaaacca
      661 caggctaaat caacatttcc aacagcaaca agcggttatg attttagaaa atcagcttgc
      721 cctcaaatta gccgggttag attgtgaatc aagtattact caaaatcaga ttcgctatca
      781 gattcaatgc tctggcaatc gcttaaccat tcgctttccg ttaggcaaaa ttgagctgaa
      841 taatgattga atcgtccctc gctttggagg acaataattc aattagcgag gcgttattat
      901 gtttacactt taccaatcca accaaatttc atcactggcg gagatgctgg tgaaaatcca
      961 gcaggtaaac ccacttgaag atccgtttga gccggaaacc attttgatcc aaagtcaggg
     1021 aatggcacag tggctacaaa tgcagattgc tgaattaaac ggtgtgatgg ggaattgcga
     1081 ttttctctac cctactacat ttttatggca acagtatcgg ctgttgttcc cggagctgcc
     1141 gaaagagaat atttttgagc gtagttcgtt ggtttggcgg attatgcggt tattgccgag
     1201 ttgcttgaca ctatcggagt ttgctccgct aaagcgttat ttaggtgatc aacctaataa
     1261 agaacagtat cagcttaagc tctatcaact ttctgccaaa attgccgaat tgttcgacca
     1321 atatttggtg tatcgccccc attggctggt tagctgggaa gagggcaact tacaagcgat
     1381 cgaaaatgag ataaaaattg caactgccgc taagccgatt gattttgact taattcgagc
     1441 cgatatgaaa tggcaaagcc atttatggca acagattgta tctgatctgc aatcggaatc
     1501 tgatgagcag gtttttacta cctcccaccg tgcctatttg caacaaaatt acttcgctaa
     1561 gttagatgac cttactgaaa cggaaaaagc caaattgcca aaacgcattt tcatttttgg
     1621 gatttcgtcc ttctcgccat tacagttagc ggtgtttaag aaactcagcg agcattgcga
     1681 tattcacctg ttctttttta acccaagcga aaaatattgg ggcgacagtg tggaagaaaa
     1741 agtgtggcaa aaattggcgt taagccacaa gatttcgcaa gacgatctgg ataacttatt
     1801 ggcggagcaa agaaataaac tactgaatct ttggggcaaa caagggcggg atttcttagc
     1861 tcaattaatc gaatttgagc cgaataccat ggatgtcttt gtcgaaccag aggaaaacag
     1921 caacctcaat aaactcaaaa aacagatttt cagcttagaa aatgacgcca ctattaccga
     1981 tttaaccgcc gataattcac tccaaattca ttcgtgccac agcccaatgc gggaagtgga
     2041 agtgttgcat aaccagctcc tgaatttgtt tgagcaagat agcactttgc tgccgaaaga
     2101 tattattgtg atgtcgccgg atattaataa atacgctcct tacattgatg cggtgttttc
     2161 tcgctacaat acgggtaatc gggatcctcg ctatattcct ttttcattat ccgaccaagc
     2221 atttacgcag gttgatccga ttatccacag tttcctgcaa ctgctggcaa tgaaagaaag
     2281 cccgtttaat gccgaagaac tgtttgattt actggaagtg aatgcgatcc aaacccgttt
     2341 tggctttagt acggaagata ttcataccct gcgggtttgg gtgagaaatg ccggcgtacg
     2401 ttcgggtgta gaaatcgagc aacctcactg gcaaaactac aattcgtggg aaaacgggct
     2461 aaaccgttta ttgcttggct caagcctgaa agaagagcag gggatttggg aaaacaccct
     2521 tgcctttggg gatagctatg gcttaaatgc cgagctggtg gggcgacttg cctcttttat
     2581 tgaggcttta ttagcgtggg taaactttct gcaaacgcca cagccgttgg cagcgtggca
     2641 gcaggcaata aaaaatttga ctgctgatct gtatcaagaa aatgccgaaa atgctgcatc
     2701 aattttattg ttgaataatg tgaatgatca gattatcgag caaatttccg caacccattt
     2761 ttcagacgat ctgcaaattg aggttttagc cttattgttt gagcagcagc tcagccaaca
     2821 ttcacagcag cttaatttct tggtcggacg ggtgaatttt gccaccttgc ttccgatgcg
     2881 ggcgattccg tttaaggtaa tttgcttgct gggtatgaat gaggcagatt tccctcgcca
     2941 acaacaaatc aatagctttg atttaatgca gtacgccccg aaaaagggag accgtgccaa
     3001 acgtgatgat gaccgctact tgttcttaga agccttactt tccgctcagc aaatgtttta
     3061 catcagctat atcgggcagt cgcaaaaaga cggcaaagag atgctaccgt cgattttggt
     3121 ttcccaactg ttagatgcaa tgaatgaagc tttaacgcct gaatgcaagc aacaattaag
     3181 cgatgaagtg gaagttttgg tcagaaaaca acctttaagt gtgtttagca aaaagaattt
     3241 tacccaccca cgagatcacg cttataacgc agagtggatt atcaagccga ctgttgccgt
     3301 acaagatttt cttagtgagc ctttaaccaa tgaaagcgtg gcttcgctag aacttgatga
     3361 attaatctct tttgtacaaa acccgattag gtttttcttt ctaaaagtgt tggggatttc
     3421 gtttaacact tatgatgaca ccattgatga aagcgaaatt tttgagatgt cggggctgga
     3481 taactaccgg attttaaacg aattattaac gataaattta gcacaacagc aacgtttttt
     3541 tgccgaagag caactcaaag gcaatttacc tgcttcttat tttgctcaaa ttgccacaca
     3601 agatttgatc ggcaaaatta ccgaaatgca agcggcgtta agccactatt tagccagcga
     3661 aagccgtatt ttgagtgtta atcgggaggt tgagctggaa cgccaaaccc tcaccctaaa
     3721 cggcaatatt gccaacctat atcagaatga agtggtgcta tggcgagtag ggcatttacg
     3781 agataaagac aagattcaga cttggatcta ccatttatta ttgtgtgcct atttccctga
     3841 aaaaacggtg aaattctatt accgagaggg tgagcaagtt ggtgtgttga gctttgaaac
     3901 cgtctcgcag atagaggcat tatcgcaatt aagcatttat ttgaatgctt atttggacgg
     3961 attatcgcaa gcccagttag tgatctacga ccgaattgag gaatatctca aaggtgatgg
     4021 cacaaacagc gaacaagttc ttatcgattt agcagagaaa gatgagcgag acggcagcta
     4081 tctccatcgg cttctcgctc aaactaaaga gctggattct caagcgattc accaacgtac
     4141 tcaaagctgg tttagcttaa tgaatcagaa aatagcgaaa ggcgattaac tacaagcggt
     4201 cgtttttggc tgaaaatttg caaaatgaga aggcgaataa taattcgcct acctataaaa
     4261 atagaatttt ttccgtattt tactgtcaat ttattactac aatgctaatt tcaaaaggag
     4321 atgtatgaaa ttatcttttc ttacccaatt cgttgcagcg gcttttgccc tcaatgtgat
     4381 gacttaccct gcatttgcgg aagttgcaaa aaattctgaa aaaacgaccg ctagtcaacc
     4441 ccaatcccaa ctcggttttg agctgattaa acagagcatt aacaaaagcc cgaatgataa
     4501 agcgatttat caaggtatta aacttgataa tggaatggaa gtgctactga tttccgacag
     4561 tcaagcgaat aaatcgttga tgtctgccat tattccgatt ggctcaatgg atgacccgat
     4621 ttctcagcaa ggtttagccc actatcttga gcatatgatc ttgatgggct cgaaaaacta
     4681 tccggaaacc aacagcttag acggtttttt aaccaaaaat ggcggtatga ataatgcctc
     4741 gactgcttct caccgcacgg cgtattatct acaagtcaat aacgaagcct ttagcgaagc
     4801 ggtaacacga ctggcggata cgttagcttt cccgttattg ctggaaacca acgccaagaa
     4861 agaggttaat gcggtcaatg cggagatggt gcgagctaaa tcgcacgatg ggcatttatt
     4921 gcacagtgtt aatttagcca ccgcaaaccc ggctcatcct gctaccaaat ttgcggtagg
     4981 caataaagag acgttatctg acaagccgga tagcaaatta caaaccgagt tggagcaatt
     5041 ttataaacag cattattctg cgaatctgtt taaagtggtg ctttattcca atcaatcgat
     5101 tgagcagatg gcaaaattgg cagccaacac tttaggcaaa atggaaaaca aacatttcag
     5161 cataccgcaa accgctgagc cacgttatcg tgatgaggac aaatcggtat ttattcaata
     5221 taagccggtg aaatccaaca aaatgttagt cattggcttt gattttccga atgacgaaat
     5281 gcagtttaag cacaaaaccg gtaaatatat tgcttatatg ttaaacaata atactgacgg
     5341 cacgctttcg gattacttga ttaaacaggg gttgtctgac ggcggtattg aggcggttag
     5401 cagtgccaat atcgggcggg atcgaggctc gtttgacatc tatattgaac tgacagacaa
     5461 aggtttgagc gagcaagatc aaattatttc attggttttc caacaaattg agaagatcaa
     5521 acaagaaggc attcaagaga gctattttag cgaagtgaaa gagagcctga agcaggattt
     5581 ccaacatttg caagtggaaa aaaatatgga tttcgtggaa tttattgccg atcaaatgct
     5641 ccatttcccg ttagaacacg ttatcgatca gccttatctg accgaaacaa tggataacga
     5701 ggcaattaaa gcaaaattgg cggcaatgaa tattgataat gcccgcatta tgttagtcag
     5761 cgatactgcc aaaaccgaca agaaaacccc ttattttgaa gcaggttatt cggtggcaaa
     5821 aatcacgcaa gaacaaaagc aaaaatggct ggatttcagt aaaaatccac agctcaattt
     5881 gcctgaattg aacccgtatt tcactaccga ttttacctta aacaaggtcg aaaccggctt
     5941 aactaaaccg aaattgatta acgaccaagc ggggaattta atctatacaa tgccgagcgt
     6001 gtattttacc aatgaaccga aggctaaaat cggcgttggc tttaatattc gcccgagaaa
     6061 agatgactta aagagcctga ccgcagcgtc tattttgggt tatatgaaca atttagccca
     6121 aaccaagtta gattttcagt cttcggtggc gggtatgaat ttaagtttag ctccggcggc
     6181 aaacggttta gccttaagtg cagagggtta tacccaaaat ctggctaaat taatgctaga
     6241 cagcgtaaaa cagttcagcc gctttgagtt taacgaagac gtgttggcac aggctaaaca
     6301 acgtttgcac caagagctgg atcgtaacga aaaagaaaac agccttaatc aagctaatgc
     6361 agcattaagc cgttttgcct cttaccctta ttttgatgtg ccgaaacagc gtgagatgat
     6421 tgatagcatt acccttgcgg atattgagca actacgccag cgaattttgc agcagagcag
     6481 cggctttaag ttgttatcgg tggggaattt gtcggctgag caggtttctg ctctggcaga
     6541 ggatttacaa gcggtcataa aaaaccaaaa atctgcaaat gctaccggac agtatgtgga
     6601 tgtgagtacc agtcagcgta agattaatgt gattaaaagc gtgccgaatg aagataatgc
     6661 gttgctaatg gcatttatgc cgaacggcta taacgagctg gaaagcctta ctcgagccaa
     6721 attgctcaga agcattattt cccgttggta ttttgatgat ttgcgtactg ataagcaact
     6781 aggctatgtg gtttatgcga cagataatat catcgggaaa accgccggta tccagtttat
     6841 ggtacaaagc ccgaatacca caccggcagg gattttagag cataacgaac gtttctttac
     6901 acaaagtttc gaacgtttaa aaaatctatc tgatgaggaa tttggaaaat atcgtgatag
     6961 cctagtggaa aaactgcaat ataaaccgga gtctttagcg gaagagtttt ctgagttcgg
     7021 ttcagacttt agcagaaata acccgaaatt tgaccgcttg caacaggtga tcgaattaac
     7081 caaagcactc accaaagcag atattgtggc attttaccaa aaagctgtga ttgaaaagca
     7141 aggcttcgtg tttgtcagcc aagctgtcgg caccaaaacc aaagctgaag acgttgcgaa
     7201 atttgacggt tttgaacaga tcgaaagcat tgaaaagcta caacgagagt ttgagattaa
     7261 ggattattag agagttatta gagagagtta ggggcgaaaa attttcgccc ctcatttttt
     7321 aatccacaat tttcaccgga tataagtaag tatatccgaa cggactttgt ttgtagcctt
     7381 caacacgttt gctggttggg gcaaagttaa ttgagtgagc cacgttaatc cacggagctt
     7441 gctctcttaa gataacctgt gcctgctcgt ataacttagt acgttctgct ttatcagatg
     7501 agcctaatgc tttttctaat aacgcatcta actcagggtt agtaaagcga gcatagttac
     7561 tgttgccgat atttggcgtg ccgaatagtg gcgataagaa gttatccgga tcgccgttat
     7621 cgcccgacca accgtaagtt ccggcggtta attcaccggc tttggtacgc ttgatgtaat
     7681 caccccattc ataggtgact aattttgctt ttacgccgat tttttcccag tcggcttgaa
     7741 taatttcaga catacggcgt gggttcgggt tagaagcacg caccacaggc tgcacccata
     7801 attcggtttc aaaaccgttc gggaagcctg cttcagctaa tagttgcttc gctttttcca
     7861 cgttatattc tgaatccgct aagctgtcgt tatagcccca aatggttggc ggaagtgggt
     7921 ttttcgccgc aaaaccgtta ccttggtaaa ctacatcaat aatcgctttt ttatccactg
     7981 ctaagtttaa cgcttggcga actttcacgt tatcaaacgg agctttttcg gtattgaatg
     8041 ccacataagc aatgttcaaa cccggttgag aaagtaaatt gattttcgga tcatttttca
     8101 ttttctcaat gtcggttgca tttgggaagt ccattaaatc acattgaccg gattgtaatt
     8161 tcgcataacg ggttgttgca tccggcacga tctcaaaaat caaacggtca atgtcaggct
     8221 tacctttcca atactctttg tttgctacat aacgactggc ttggtctagc acataaccgc
     8281 taaacacaaa cggacctgtg ccgattggcg tggtatctac cgcttcaggt atgccggctt
     8341 tcatcatttt atccgcatat tccgccgaat aaatggaggt gaaatccata ccaaggctgg
     8401 ataagaatga cgcatcacgt ttggttaagg taatacgaac ggtgctgtcg tccacttttt
     8461 ccaccgattt caataatgtc gggaatttca ttgcgttgaa atacggataa gtgccgttag
     8521 agacggtgtg atacggatga tttttatcta actgacggtt aaatgaaaat actacatcgt
     8581 ccgcattcaa ttcacggctt ggagtgaagt ctttgtttga atggaatttc acgcctttgc
     8641 gtaatttaaa ggtgtaagtt aagccgtctt cgctgatttc ccagctttct gccaatgccg
     8701 gttcaatatc ggttgtaccc ggcacaaatt ccaccaagcg gttgtaaatt tgttgcgagc
     8761 tggcgttata agataaaccg tccatcatta aaatcgggct aaaaccggtt ggcgaacggc
     8821 taacgcagtt tacaaaggtt ttctcggttt gcggttttgc tgctgccgca gtaggtttat
     8881 tttcgttgct tttatcatcg caagccgcta gagctaatac cgctaaaacg cttgcactaa
     8941 ttttggttaa cgttggaaat ttcatattat tctccgctta atcttaggat taacttacga
     9001 agattacctt ttccacaatg aggagtaaag aacaaaaagc attatttttg caaatcaatc
     9061 catagaatta gataccgtca acgtcattgc cacccgtgat ccaagtaaat ttgccgatac
     9121 gccacaaaaa caaactaaag atgcactttt ggtaaaacaa gcgacgagtg tggcggcggc
     9181 attaaaagcg ttgccgaatg tggatgtgca aggaggggcc cgtgcgattg tgcagaagcc
     9241 gtctattcgt gggttgagcg ataatcgtat tgtgcaggtg attgacggag tgcgtcagaa
     9301 ttttgatttg gctcaccgtg gttcttattt tgtgccgatg tcattgattc aagaaattga
     9361 agtaatcaaa ggtccaagca gctcgttgtg ggggagcggt gccttaggtg gggtggtggc
     9421 aatgcgcaca ccgaatgcgt tggatttatt gaaaaataat gacaaattcg gggcaaaaat
     9481 ccgtcaaggt tatcaaacgg cgaataatct ttccgaaacc gaagcctcgg tgtttgcagc
     9541 aaatgatcgg tttgatgccc tgctgagtgg tttctacaat aatgctgaca atttgcgtag
     9601 cggtgaaggc aaggaactga ataacacggg ctataagcaa gccggcggtt tagtaaaatt
     9661 cggctggcaa attaacgata ctcatcgttt ggaattatcc catcgtcata gccaatttaa
     9721 acaaactgca ccgggtaata acgaggtaga aaatcaattt accgataatg atgttagtgc
     9781 acaaattagt gcttggcatg cggctaatcc tggaaatcca agtaatccag tatatcttgc
     9841 gaaaatgtta gccttttacg gtcaattaca ttcaagtctt ggtagtgtga gttatttaag
     9901 tgatcaaaaa attaccgata aaagcacctc acttaactac tatttcaacc caagcaatcc
     9961 ttatttgagc acacaagtga cattatatag caatagtacc actgaaaacg aacatcgaaa
    10021 aataagtggt gtggaagatc aaactaaact aagcactcgt ggtgtgaata tccgtaatca
    10081 ttccgaattg tcttggttct ccttggtgta tggcgtggat tatatgttgg ataaagtagc
    10141 cactcagcgt gggagaaata atacggacgc acaataccgt gctaatcctt atgatgctaa
    10201 atctaaaact acaggtgctt atttaattgc acatattccg ctgtttgatg aaaaagtggt
    10261 cttttctccg agcgtgcgtt acgaccgttt cgatacttca agtgaaacgg tgaaatataa
    10321 agacagccat tggtctcctg ctgcaaaact tacttggaaa gcgacaaatt ggttggattt
    10381 aaccgcgaaa tataacgaag ctttcagagc cccttctatg caagaacgat ttgtgagcgg
    10441 gtctcatttt ggtaccacag tgagaggatt acctattaat aatattttct taactaaccc
    10501 taatttacgc ccggaaacgg caaaaaataa ggaagtaacc ttcaatgtac attttgataa
    10561 tgtcatctcg caaggagata aatttaactt tgaagcgact tatttccgta atgatgtgaa
    10621 ggatttgatt aatcttcaag tattcaaagc aaatcctaca agcccaatgg aatttattcc
    10681 cactcgttcc caatatcaaa atattgcgaa tgcacgtttg agtggtattg aattacaaac
    10741 tcgttatcaa actgaacgtt tggcagtttt tgctaactac ggtagtacaa aaggtaaaga
    10801 taaagacagc ggggaagcct tgtcgaacat tgctgcaagc aaaatcggct tcggggtcaa
    10861 ttatgccgtg gtgcaagaca aattcacggt gggggcaaac gtaacccgtt atcaagcaca
    10921 acatcgtgtg ccgaaaaacc acggcgtgac ttaccaaggc tatacgctga ccgatctaca
    10981 cgccacttac gccccattaa aaggcgaatg gaaaaacctg cgtttagacg tggcggttga
    11041 aaacctattc gataaaaaat accaaccggc attcagctta atggaaggca gcggtcgtaa
    11101 tgtgaaactc agtgcggctt acagcttcta atgtctttaa cggaagacgg cacaagcggt
    11161 cgttttccgg ttattttttg caatattaaa agggagttac tatgaaattt tatccgtcgc
    11221 tgatcgcaag tgcggtcagt tttgccgttg ttttgagtgg acaagcggca gtgaatgtgc
    11281 cggatgccgg ctcgttacag cgtgaattag accaacgtca agctcaacaa aaagtcaaac
    11341 cgagtggcga atggtttaat caagcggcaa aagcacctta taccgcaacg gacggtcaag
    11401 aactgacatt tccattaact caggtacaag tttttgatcg aaccaaccaa gaaatctcta
    11461 ccccggaatt agccccgatt ttggcaaact atgtgggtaa agcggtttcg ctgaccgatt
    11521 taagcaactt ggcaaatgag atcaccgagt tttatcgtag ccacaattat ctggtggcaa
    11581 aagccatttt accgccacag gaaattgaac aaggtaaggt gaaaatcctg ttgtttaaag
    11641 gcaatgtcgg tgaagtgcgg ttacaaaatc agagtgcttt aagcgataaa tttgttgcac
    11701 gccttgccaa caccacagtg aatggttcgg aatttatcct aaaagacgaa ttggaaaaat
    11761 ttgccctcac gatgaatgat gttcccgggg tgaataccgg cttggagctt tctgccggta
    11821 agcaagcagg cgaggcgaat ttgctgatta agctccaaaa tgccgagcgt ttctcaggct
    11881 atgtatcggc agataaccaa ggcaataaaa ataccggacg ttaccgttta gccacaggga
    11941 taaaagccaa taatctgata ggttggggcg atgagttgaa agtggatttg atgagttcca
    12001 acaacgctaa tttaaaaaat gcgcgtttgg attattcgag cttgattgac ggctacgcta
    12061 cccgtttggg cttaacggcg aattatctcg actacgaatt aggcggtaac tttaaagccc
    12121 tgcaatccca agggcatact cacaatcttg gggcttatgt gttgcacccg acaatccgct
    12181 tgccggattt tcggttaaac accaaagtca gctttaatca tcaacgttta accgacaaac
    12241 aacaagcggt caatgtggca caaaaacgcc aagtaaacag cttaacggtg ggcattaacg
    12301 ggtcgtggaa ttcgcttaaa gacggcacaa gctatttctc tctgtctgcc ttgttcggca
    12361 atttagctaa ccacaccaac gagcaagcgc attatgtggc aacagatttt cagcccaaat
    12421 cccgttttac ggtctatcaa tacagcctgt ctcacgaaca agcgctgcca aaatcctttg
    12481 ccttcaacct tggcgtgaac gggcagtttg cggataaaac cctcgacagc tcacaaaaaa
    12541 tgttactggg cggtttatac ggcgtgcgtg gtcatcaagc cggtgtcgct tcggtggatg
    12601 aggggcattt agtgcaaacc gaactcaagc attatttacc gctcttttcc aaaagcctgc
    12661 tgaccagctc ggtcttctat gattatggtt ggggaaaata ctataaacac agcggatttt
    12721 tggcgcgtgg tgtaaacaat agcgtgaaat tacagagtat cggagcaggg ctttcgctct
    12781 ctgaagcagg cagttatgcc attaacgcca gtgttgccaa accgctgggt aaaaaaatcg
    12841 agaatgcaga taaacaccaa ttctggttct ccgccatcaa aaccttctaa ggagacaaac
    12901 tatgtttaaa cgtaatgcaa tttctctctg cattttgacc gcttgcagcg gtttagccta
    12961 tgccaataca gcagatttgc cgcaaggtga aagggtgcgt gtcgggacgg caacgattga
    13021 gcgcacaacc gataaaatga ccgtgaatca atccagcaat aaagtacaaa ttgattggaa
    13081 cagctttgat attggtaaga ataaagaagt ggaattcaaa cagccgacag agatggcggt
    13141 ggcatataac cgtgttaccg gcggaaacgc cagtcaaatt caaggcaagc tgaccgcaaa
    13201 cggcaaagtg tatttagcca acccgaacgg tgtactcatc accaaagatg cggaagtgaa
    13261 tgtgggcggt ttgttagtga ccacaaaaga tttagaaaaa atcagcgaaa acggcacaaa
    13321 agataaattc agtcgcaaat tagcgaaaga aggcaaaatt cttaaccaag gtaaaattac
    13381 ggccaaaggc gagcgggctt ttgtggtatt ggtgggcgac gatgtggcta atgaagggga
    13441 aattaacgcc aaaaattatt accacaaaga aacgaaaaaa gtcaccgaat gttatgacga
    13501 ttcttggagc ttggtaaaac aatgctacga ttttgataaa gaaatcgtga aaagtaccag
    13561 tggagaagtg catttatcct ccggagaaaa tgtcaccttt actttaagcg accgctatat
    13621 tgatgtggcg ttagacgata atacggtcaa tagcattatc aaaaatgacg gggcaatcat
    13681 cgccaatggt gacattaccc tcactgccaa agggcgaaat caagccttag acagcttaat
    13741 tatcaataac ggcgtattgc aagccaataa ggtgcaaaat aaaaacggta cgatcatttt
    13801 atctgccggt gaaacccaat tacacgaaaa aagccaaatt aaaggcgaga aagtgagctt
    13861 tgccgaagct tcttattacg gtccgacaga agacattaaa gtggtttcca aaaccggctc
    13921 aaaagtgacc gcacctgtga tcgatttcaa aggtaaaacc gtgaatattc aaggggaatt
    13981 tgggcgtgaa gacagcaccg atctctataa cgatgaacta caaatcctca aaaccacggt
    14041 gaatattgaa gtcccgaata cggaagatat tcgcattgca gataccgaca acggaggtag
    14101 cggttccttt atacaagcgg gtgctttagc ctcattactt gcaaataacg gacgggttaa
    14161 tgtacagggc aaaggttttg atgtctcagg caagttgaat gtgaatgcct ttaaacaaac
    14221 ggattccgtg ctgaaattta tcaatcaggc gggcatcaat atccacaatg ccgatattca
    14281 cgcaaacggg cgtttattct ttgtgacaaa cttgccgaat gacaccgatt tccaatcgaa
    14341 tatccgcatt accgacagca aaatcaactt aggcaacggc tcaatgggct ttggtcgcag
    14401 tagcaaacgt tgggaaatgc acgatgtggt tcgacgtgcc gatacggaac tgcgtaaaaa
    14461 attcaatctg gatatgaaaa acgtggaatt aaaccaagtg gatgattttg tggtagcggg
    14521 cggtttccaa caagttaatt tggataacgt gaaagcccgt ggcaaaacca acttctatct
    14581 ggattccggc aactcccgca tgtttgtcgg tgagcagatt tatgaatatg gcgtaaccga
    14641 tattgaaaaa cgcacccaat tggcgaattt agaccagcgt agaaaacgtt ggaattcgga
    14701 taactatgat gagaccgatc tcaactggga atacaaccaa cgctttgacg gctttgcagc
    14761 aaaacacaat caccgcgcgc cgattgccga tacggaaatc aatattgcca atagtgatat
    14821 cagcttagat aacggttttg tgcatttaat ggcagaaaaa atcaatctgg acgacagcaa
    14881 aatcaatatc accttcaacc aagacaacag cttggatgaa agtacccaaa tcaaccgctt
    14941 aggactaaac ggcaaagtgt cgatgaaaaa cagccatatt aacgtagtgg gcgatgaaaa
    15001 agacggtatc tcaccggctc gccattatgc cacgatgttc ttagtggggg agctgcttgg
    15061 tgaaaagagt tctattttcc tcaaatccca ccaaggtgca gtattgagaa ccgacagcaa
    15121 tgttacaatt gccggtaaaa acagcaaaga cgacctcaaa attaccgcca ttaccaccgg
    15181 taaaaaaatg ggcaatgaaa tcatcggacc ggacggcttg ccaacctcgg aagataacga
    15241 tgccaacgcc agcaacaccg cctttgccat cggctatgaa cctaacgcta cggctatcgt
    15301 cgaaaatgca agcattaccg cattagcacc aaacggcggc ttggcataca ccggcacgtc
    15361 taaaacccaa attgacgcga aggataatgc tacctttacc ttctttgaaa aacctcgagc
    15421 cggctatgac aaattgcttg gcgacatcaa aggtaacccc gataaattaa ccccacgcga
    15481 tttcgcccgt ttatacgaca acattaaagg catcaaagcc agcagcctat ccgaacaaca
    15541 gcttggccta gcagacaccg cgaagaaaac cagcgaacac accttagcaa acagcctaga
    15601 cgtagaaaat ttggtttcag tgacagtgtg tgatgagcag aataagtgtg aggagcgtat
    15661 gttaggtaac aaaaatacca atgctaaagt tattgtcgga gatatcaaat ttgagtagcg
    15721 ttgaagaaag tggtagaagc tcagacttga tctgataaag cactataaaa agtgagagtt
    15781 tcccgtttag aatatgagtg tcaaaattca atctaaacaa aaaggaaact ctcatgtttt
    15841 attacttaca atcccctcat taaacacaag accggtttat taaatttagc agaagaaccg
    15901 ggcaatatct ctcaagcctg caatgtggtg ggtatgagcc tagatacatt ctattgctat
    15961 caacaagcgg ttatttttgg caactaattt gcaaaaaaac aataaaatcc gaccgcttgt
    16021 agagggttaa tccgccctta gtgaaatttc accgatgccg aaatagcgga ggttgtcgcc
    16081 ctgttttaga atgagttcgc cctgctcgtt aatgccttgc gaaatgccgt ggatttcttc
    16141 ggcttcggta atgagcttaa ccgctttatg gcggaaaagg tcgaaacttt gccagcgttc
    16201 ggcataatga gcgaagccga caagcggata aattttgaga tttttttgca attcataggc
    16261 taaacggcaa accaattcgt tgcggttgaa atgatattgc gataaatctg cccacgcttg
    16321 cgtcacaata ttttcatcca cttttgccat tcctaggttc agcccaatgc cgataaccac
    16381 atctaaacca ttgcgattgg cttgggtttc caacaaaatg ccccccattt ttttgccttg
    16441 gtaataaatg tcattcggcc atttaatttg aatatctttt acattttgaa cagtaaggct
    16501 ttccgcaatg atcaaggcta ccactaaact taagggcggt aaatctgccg cctgctccat
    16561 cgggtaatgc cataaaatcg agaaatagag attttcactc tcgggcgaat accaagttcg
    16621 acctcgtctg ccacgtccgg cagtttgttt ttcagcgagg caaaccgtgc cgttttccaa
    16681 acgttggtga tgagtgagca aatattcatt ggttgaatca atttcatcga acacaacggc
    16741 ttgcccgcaa attaaagcat tttggatttg agcttggtta agtttcatta tttccccact
    16801 tagcgagtga ttttttcgct atcgacctca ccgtttttac cgataaaacg cacttcaggc
    16861 tgaatttcta ccccaaattt ttctttcact ctctggcgaa cggttttggc tagagccaag
    16921 acatcttgcc ctgttgcatt ttctttatta atcagcacta aggcttgctg ggtatgtacc
    16981 gccgctccgc caatttgtaa gtctttcagc ccggtttggt caattaacca acctgcggct
    17041 aattttaccg ttccatcagc ttgagggtag tgaggaatgg ctgggaattt ggcttgtatt
    17101 ggtgcaaatt gttcgtttga aatcaccgga tttttaaaaa agctgccggc attgccaaat
    17161 tcgtcagggt ttggcaattt ggaggagcga accgcacaga cttcatcaaa aatctgtatt
    17221 ggcgtaacgg tttccggatt gagttgggct aatgatccgt aacttagcac cggtttccac
    17281 gctttggcta actttaagcc taccgagata atcgcaaatt catcacggta ttggtgttta
    17341 aacacacttt cacgatagcc aaattcgcac tcggatttgg atagcttaaa gagttctccg
    17401 gaacgtaaat ttaacacttc cacgaaatca catacctgtt caaattcaac cccataagct
    17461 cctatatttt gaatgggagc agagctggcg acacccggaa ttaacgccag attttcaatg
    17521 ccggcaatat tgtgtgccaa cgtccattta accaattcgt gccagttttc cccaccttga
    17581 acgtgcaaat agtggaaatg ttcgtcttcg tggtgcgtaa tccctttgag tttatttact
    17641 aacaccacac cgtcaaaatc ctctaaaaac aaaacgttag agccttgccc gagaatcaag
    17701 attggcagtt gagcgttaaa ggctttttgc cattcgctca atagctgttc gactgtggta
    17761 aattcaataa tttgggtcgc ttttgcaggc aaatggaaag tgtgaaaagg ggttaagcta
    17821 tgtttcatca tcaaattagt tagttaagaa gtggaaataa caagcggtga tatttttaca
    17881 aaattttgca attaagattt tgggcaggca caggatctgc ccctacattc agaattacct
    17941 tgccccatat accacaatgg ttttgccgtg agcagaaatt aagccttcgt cttccaacat
    18001 ttttataatg cgtccaacgg tttcacggga acaaccgacc atttgcccga tttcttggcg
    18061 ggtaattttg atctgcatac cttccgggtg agtcattgca tccggcattt tagctaagtt
    18121 gagcaaggtt tgagcaatgc gaccggcaac gtctaaaaag gcaagattac cgacttggcg
    18181 tgaagtttga cgtaaacggc gagccatttg tgccgataaa tacattaaaa tatcggggtt
    18241 taaatggact agttgtttga attttttata ggaaatttcc gcaatttcac aaggaccttt
    18301 agtgcggacg taagcggtac gttgttggac tttttcttca aataaactta attcaccgac
    18361 aaactcacct gtgcctaagt tagttaagag catctctttg gtttcatcat ctttgatgta
    18421 aaccgacact gagccattca cgatgtagaa aagtgattct gcatcttcac cggcgtgaat
    18481 gagtgtatat ttcgccggat aacgatggat atgacaatgg gttaaaaacc actccaccgc
    18541 agggtcgtga atagagggcg gaggagttac attttccaga ggagctgttg aaagtgtcat
    18601 atcttgcata gtatttccta taactcttat actaggttaa atttttagaa ttttaacacg
    18661 aaattaaagg ttagcctaaa aaagattttt tgtctttaat atcaaggctt tcgtgtaatt
    18721 ccgaccaaac aatcaccact ttatctgcct taagttgtgt gagtaatcgt tctctttttt
    18781 ctgccagaga aagttccctt tccccgtagt ctgtgccttc tcgtaaaatg aaggaatcta
    18841 aaatattatg taaggttgat tcttcgagtt cttgccaagg tataatcata attaatcaaa
    18901 taaaggctta ataaatgccc actgtttttc aaaatgttgg tgttggttat agctgaaatt
    18961 agaacgaacc aagcgtaaaa attgcccttc gcagaaattc actaaataac cggctaaggc
    19021 tctttcgtct tcaaagctct tgcgttcacg cagtttacta agttgcaaaa tattggcaaa
    19081 ttgtaactcc aagttatcaa agaattttgc cactcgagct ttgagcaact cctcttcaaa
    19141 catcaaggcg tgtccggtca ggatgcgggt aacgcccggg tttttgcgag caaattctaa
    19201 aatcacatat aaaattgctc gcaccgaatt ggcactgctg tcttggcgtt tattggcgtg
    19261 aatatagctg gtgagggtct gctcgatttt ctcgattaac gcttcaaaca tcttggtttt
    19321 acttgggaaa tagcgataca acgccccctc cgacaccccc accgcagcgg ctaaacgctc
    19381 ggtagtgata cgctgcattc cctcttcaga atttaacaag ctgattaaca cttccaacac
    19441 ttgttggcgg cgttccacca ctgatttttt cggcatttta attttaggtt ctgtcattgc
    19501 caatcctatt attgcttacc tgaatgccca aaaccgcctt cgccacggtc ggtctgctca
    19561 aactcttgca caatgttaaa acttgcctga accaccggca caaagactaa ctgagcaata
    19621 cggtcgccca cgttcacggt aaaatcagct tggctacggt tccaaagcga cactttcagc
    19681 tcgccttggt aatcgctatc gatcaagccg actaagttgc ctagcacgat cccgttttta
    19741 tgccctaaac ctgatcttgg taagatcacc gctgccaaat tcggatcggc gatataaatt
    19801 gaaatccccg ttgggatcaa caccgtttca cccgctttta ccgtcagcgg ctcgttgagc
    19861 atcgcccgta gatccaagcc tgccgagcct tcggtcgcat acgcaggtaa tggaaattca
    19921 ttgccgatac ggctatctaa aatttttaaa tcaatttgtt tcattctttt tcctttttgt
    19981 aaatttttaa aactatatta ttcagttata aaaggtttta attgtatcgt tccaagtgga
    20041 agttcagtca atatttccca gcttgtgttg tggtaaaatc gaaataaact tgcaacgttt
    20101 tcaacgtagc ttgaattaaa tttaccttct aatggcttta ttcctccagt aaaat
//
LOCUS       DANLZC010000031        19582 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.031, whole genome shotgun sequence.
ACCESSION   DANLZC010000031 DANLZC010000000
VERSION     DANLZC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19582
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.031"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            16..2625
                     /gene="adhE"
                     /locus_tag="PXE44_000880"
                     /gene_synonym="adhC"
     CDS             16..2625
                     /gene="adhE"
                     /locus_tag="PXE44_000880"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL5588407.1"
                     /translation="MAKNTQPFDAQAEVNELVEKGLKALDEFRKLNQEQVDFIVAKAS
                     VAALDKHGILAMHAYEETGRGVFEDKATKNLFACEYVVNNMRHLKTAGVISEDDVTGI
                     TEIADPVGVVCGITPTTNPTSTTIFKALIALKTRNPIVFAFHPSAQQSSAHAAQIVRD
                     AAVAAGAPENCIQWITQPSMEGTSALMKHPGIATILATGGNAMVEAAYSCGKPALGVG
                     AGNVPAYVEKTAKLKQAVYDIVMSKSFDNGMICASEQAAIVDKEIYAEFIKEMQSYGV
                     YLVNKKEKALLEKYIFGIDKAKDENCSGAKLNSAVVGKPAAWIAEQAGFKVPEKTNIL
                     LAECAFVGEAEPLTREKLSPVLALLKSDSTEHGLELSEAMVNFHGLGHSAAIHTENKA
                     LAKTFGERVKAIRVIWNSPSTFGGIGDVYNSFLPSLTLGCGSYGKNSVGNNVSAVNLI
                     NIKRVGRRRNNMQWFKVPSKIYFERDSIQYLKSMHDVHKVMIVTDRSMVDLGFVDRIT
                     EQLRQRRNQVMIQFFTDVEPNPSLQTVQRGTELMRSFQPDTIIALGGGSPMDAAKIMW
                     LFYEQPEVDFRDLVQKFMDIRKRAFKFPQLGRKAKFVGIPTTSGTGSEVTPFAVITDG
                     DIKYPLADYSLTPTIAIVDPALVMSVPSHVAADTGLDVLTHATEAYTSILANDYTDGL
                     ALQAIKLVFENLEKSVKEFDETAREKMHNASTMAGMAFANAFLGICHSMAHKIGGKFH
                     TIHGRTNAILLPHVIRYNGTRPTKVATWPKYTNYVADVRFQDIARMLGLPAETPEQAV
                     EAYAQAVHDLAVRCGVKMSLREQGVDEQAFLNARRELALHAFEDQCTPANPRLAMIED
                     MEAIMTKAYYGKE"
     gene            2772..3668
                     /locus_tag="PXE44_000881"
     CDS             2772..3668
                     /locus_tag="PXE44_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDL5588408.1"
                     /translation="MQITILDGGISRELMRRNAPFHQPEWSAAALYEGPHFVQAVHED
                     FIAHGAEVITTDSYAVVPFHIGEKRFAADGKMLADLAGRLAKQAVKNSGNIATQIAGS
                     LPPMFGSYRADLIQMERFAEVAQPLIDGLSPYVDIWLCETQSAIIEPISIKALLPKDN
                     RPLWVSFTLTDDEPTTEPQLRSGESVKQAVEEMVKLGVQAILFNCCQPEVIGEALSVT
                     QKTLAELKATHIRTGAYANAFAPQPKDATANDGLDEVRQDLTLEAYLGWAKKWTEQGA
                     TIIGGCCGIGVEYIETLAKNLK"
     gene            3678..5087
                     /locus_tag="PXE44_000882"
     CDS             3678..5087
                     /locus_tag="PXE44_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL5588409.1"
                     /translation="MSNKKIGLISLTALVLSSMIGSGIFSLPQNMAAVAGAEAITIGW
                     LITGVGIIFLGLSFFFISRLRPDLDGGIYTYAREGFGELMGFMSAWGYWLCATIGIVG
                     YLTVAFEGLGVFTDNPNTVIFGQGNTLASFIGSSIIVWLVHALIAGGIKEAAFVNLIA
                     TFVKVAPLILFIGLGIWYFDVDLFNSDVKATALNNSVSDQVKSTMLITLWVFTGVEGA
                     SVLSAHAKKRSDVGLATVLGIIIALALYVAITILSLGILPRETIANMANPSMGPLLDA
                     MMGPTGKVIITACLIVSVLASYISWTMYSAEVPYRGSKNGAFPKILDKVNKNGTPINS
                     LWFTGFIVQFCLVLVFVFEQSYNTLLLISTSMILIPYFLIGAYLFKLAIQTNATWYIK
                     LTGFMAALYGLWIVYAAGLEYLLLSVVLYVPGILLFLYSNYKFNGRLTLSTAEKVILI
                     VLLVIFGYAVIELPALLAA"
     gene            5194..6417
                     /locus_tag="PXE44_000883"
     CDS             5194..6417
                     /locus_tag="PXE44_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HDL5588410.1"
                     /translation="MQTYQHKALCRVRTITLFLSLTKDKLQWETALNAAKAEFDLLVP
                     AIQTQGYHIQSIRIVTNSFGEYLDLTNIETAKQDLAYLKQLLTQLNDSGLRIRFAIGE
                     AKTPHELALLPELILNYGDLSNACVNIEPDEFGILDNETILQSAQIIKQIAEITPRGE
                     GNFNFTVNFNCKPFIPYFPASYHSSKLPNSFVIGLETPDLLVAVLKELKASPHNDFYR
                     QCYETMSQALQYHVDEVLEAVKSVQHKLQFAFAGIDSSAAPSKNCASMTEVYELMGVP
                     FFGAAGTVEASSLLTKVFKSIKHVPLVGFSGLMLAVTEDLGLAAGTHKAQFDIRALLT
                     YSAVCGIGLDTVPISNEATVEQIAALMRDTGTMAFRLNKPLTVRLFPIPNKSAGEVTE
                     FESDDLCNCRILAVP"
     gene            complement(6455..7651)
                     /locus_tag="PXE44_000884"
     CDS             complement(6455..7651)
                     /locus_tag="PXE44_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL5588411.1"
                     /translation="MKQVIRFIVFSTLSLFSFSTFAKTIVAIDAGHGGKDPGAIGKNL
                     GILEKSVTLAISKELKALLDADPNFKAVMTRSGDYFIQLPQRTEIARKHRADLLVSIH
                     ADSSPRGDSLKGASVWVLSNSRASDEMGKWLEDHEKQSELLGGAGSVLSSNNERYLNQ
                     TVLDLQFSHSQRAGYELGKSILAKMSNVAALAKSSPQHASLSVLRSPDIPSVLVETGF
                     LSNSTEEAKLASANYRKQVARAIYNGLVAYRAKNAVASSSVSKKSDKEDKQNKKEEKT
                     AKADLTKKPASEKQEKNTQSDKTKLKEKSKKEDKKEDSAKTNKKKEQQLKEKTALEKD
                     QKNQSKKSDSQREIKVSNSGYHIVQQDETVYSIARAYGTTPQKISELNNLKNYQITVG
                     KKLKVK"
     gene            complement(7671..8162)
                     /gene="tsaE"
                     /locus_tag="PXE44_000885"
     CDS             complement(7671..8162)
                     /gene="tsaE"
                     /locus_tag="PXE44_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL5588412.1"
                     /translation="MTDSLSFYCEDETKLLEFGQSFALELKKYLLQDQAHSLVVYLNG
                     ELGAGKTTLTRSIVRAFGHQGNVKSPTYTLVEEYQLPPFSLYHFDLYRLADPEELEFM
                     GIRDYFKPQTLCLLEWAVKGKGMIPEADFVIQIDYKNDGRQISLLPQNQTAVDILVNF
                     HQK"
     gene            complement(8162..8716)
                     /gene="orn"
                     /locus_tag="PXE44_000886"
     CDS             complement(8162..8716)
                     /gene="orn"
                     /locus_tag="PXE44_000886"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDL5588413.1"
                     /translation="MAQNEQNLIWIDLEMTGLDPEKERIIEIATIVTDKDLNILAEGP
                     VLAVHQTDDLLEKMSDWCVKTHTANGLIERVKASKLTERAAELQTLDFLKRWVPKGAS
                     PICGNSIAQDKRFLYKYMPDLADYFHYRHLDVSTLKELARRWKPEILDKFSKGNTHLA
                     LDDIRESINELKFYREHFIQLDQK"
     gene            complement(8726..9205)
                     /gene="coaD"
                     /locus_tag="PXE44_000887"
     CDS             complement(8726..9205)
                     /gene="coaD"
                     /locus_tag="PXE44_000887"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL5588414.1"
                     /translation="MSYNVIYAGTFDPITNGHLDIICKASHLFGKVIVAVAQNPSKQP
                     LFSLEERVELVKQSCTQWQNIEVIGFSGLLADFAKQQNAKALIRGIRGADDIDYEIQL
                     AQLNYKLSGDLETIFFPPSIEWRYLSSTMVREIYRHNGDISQFVPQAVKNALKIAKV"
     gene            complement(9207..10487)
                     /gene="waaA"
                     /locus_tag="PXE44_000888"
     CDS             complement(9207..10487)
                     /gene="waaA"
                     /locus_tag="PXE44_000888"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDL5588415.1"
                     /translation="MLRLLYICLSYLLQPVVLLLMWYKGRKQPAYRKRLWERYGIYDE
                     SEKPKAKGVVIHAASVGEVIAATLLIKAVSKQYPELPLIVTTVTPTGSGRVQAAFGNS
                     VSHFYLPYDLPDAIERFLNFIDPKLMIVIETELWPNLIRKVNIRKIPFVIANARLSPR
                     SAKRYGWVKGSIKDMLNQISLIMAQDEVSRDRYLALGYAPAKMVNTGNLKFDLEITPQ
                     LHQSVIKTKASLNLTERPIWIAGSTHEGEEKLILDAHKLLLEHYPNLVLILVPRHPER
                     FGLVELLIQKSDLNYVKRTENKTLSQNTQVMLGDTMGEMMLLYGLSDIAFVGGSLVKH
                     GGHNPLEPIAFNIPVVSGLYTYNFPEIFEKLREVKGVIEIESSVEALTESIQLLLKQP
                     QIGQEIAKSGLSVLQENQGALKRHLDLLASYLEK"
     gene            complement(10667..12046)
                     /gene="yegQ"
                     /locus_tag="PXE44_000889"
     CDS             complement(10667..12046)
                     /gene="yegQ"
                     /locus_tag="PXE44_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDL5588416.1"
                     /translation="MTLKYAKPELLSPAGSLKNMRYAFAYGADAIYAGQPRYSLRVRN
                     NEFNHANLKVAIDEAHALGKKFYVVVNIAPHNSKLKTFIKDLKVVVDMKPDALIMSDP
                     GLIMLVRENFPEMDIHLSVQANAVNWATVKFWRQMGLTRVILSRELSLEEIEEIREQV
                     PDMELEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYKMEEGKIDEVGQI
                     VPKYEPEIEVKNVAPTLGNGEATDKVFLYTESQRPDEQMTAFEDEHGTYFMNSKDLRA
                     VQHVERLTQMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDESLMDTLESL
                     AHRGYTEGFLRRHTHDEYQNYEYGYSISERQQFVGEFTGKRNAEGLAEVAVKNKFLVG
                     DSVEMMTPKGNIVFKIERMLNRKNENVDAALGDGHFVFLDVPADIELDFALLMRNLVN
                     TNTRNPHQA"
     gene            12174..12545
                     /locus_tag="PXE44_000890"
     CDS             12174..12545
                     /locus_tag="PXE44_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588417.1"
                     /translation="MKLKYLLATTLLAFSTFTAAQSIKTEMMQMNMQANKLMRAESAE
                     EFSEIANDFIIITEKTKGIFPNSVEGDKAAQEDYQAGMQKLIDMVNQASEKAQAGELQ
                     EAKMLISELEMIKREYHKKYK"
     gene            complement(12584..16060)
                     /gene="dnaE"
                     /locus_tag="PXE44_000891"
     CDS             complement(12584..16060)
                     /gene="dnaE"
                     /locus_tag="PXE44_000891"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL5588418.1"
                     /translation="MSDCRFVHLKVHSDFSMINGLAKVKSLVKTAAANNMVAMGLTDF
                     INFCGLVKFYGEALGAGIKPIIGADVYVRTESGNEDYFELTLLAKNNKGYHNITQILS
                     KAYQQGYVEFPMVEREWLAELNEGIIILSGGRMGDVGKALLKENRQEAEEKLAFYQQY
                     YPNHFYLSVCRTGRADEERYIKQAVEFSQKNAIPLVAVNDVVFLKEDDFDAHEIRVAI
                     HDSYTLDDPKRPKKYSPKQYFRTEEEMCKLFADLPSALANTVEIAKRCSVTVRLGEYF
                     LPNFPTGELSTEDFLVKKSKEGLEERLEFLFPDPEERANKRSVYDERLQVELDVINQM
                     GFPGYFLIVMEFIQWSKDNDIPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFLN
                     PERVSMPDFDVDFCMDGRDRVIEHVAETYGRQAVSQIITFGTMAAKAVIRDVGRVLGH
                     PYSFVDRISKLIPPDPGMTLEKAFAAEPKLPELYEADEEVKDLIDMARKLEGVTRNAG
                     KHAGGVVIAPTAITDFSPLYCDSEGLHPVTHFDKNDVEYAGLVKFDFLGLRTLTIIKW
                     ALEMINERLAREGKEPIRIETIPLDDKKSFALLLAAKTTAVFQLESRGMKDLISRLKP
                     DCFEDIIALVALFRPGPLESGMVQNFIDRKHGKEEVSYPDPQYQHECLKPILEPTYGV
                     IVYQEQVMQIAQELAGYTLGGADLLRRAMGKKKPEEMAAQREVFEKGAEAKGIDGKLA
                     GQIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTSEMDNTDKIVG
                     FYDECMNMGLTVVPPDVNSGKHRFSVNEKGEIVYGLGAIKGVGEGPIEAILAARQKEG
                     KFKDLFDLTARVDLKKINRRTFESLIMSGAFDKLGPHRAALMKNLEDALKASDQHAKM
                     EELGQSDMFGVLTETPEEVQNAYANTPKWSEQVILDGERATLGLYLSGHPIGRFLKEL
                     SHYAPNRLNELQPTFRGQMTTVSGIVMASRVAVTKRGSRIGIATIEDRSGKLDITLFS
                     EALENFGHLMQQDQIIVATGSVQFDDFSGGLKMSAREVLSLDEARGRFAKSLALAISK
                     EQLSADFIKKMTAIITPHKEGTLPLHFYYQSPEGRVLLKGGVEWRISPNEAMFSELKA
                     LLGENSVELEFE"
     gene            complement(16285..17382)
                     /gene="lptG"
                     /locus_tag="PXE44_000892"
     CDS             complement(16285..17382)
                     /gene="lptG"
                     /locus_tag="PXE44_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDL5588419.1"
                     /translation="MQFFSMNILERYIGKTILSAIFLTLFMLIGLGAIIKFVEEFRDV
                     GRGSYDGLKAAYYTFLTIPRDIETFFPIAALLGSLLGLGNLASLSELVVMQASGFSRF
                     RIGLAVMKTALPLVIFTMIIGEWGVPQTEQFARNMRSIAQSGGSMLATKDGFWAKDGN
                     SFIYINHIKNEKSLDNVLIYQFGEGQNKRELQSVLKARTANYSDEKGWVLSKVEKSTV
                     GENQIQQAKQPDQVWQTSLTPSKLGIVSLKPESLSISGLADYVGFLKDTGQDPKRFEI
                     TFWRKVFQPISMAVMMLLAISFIFGPLRSSTMGAKIVIGIVAGFMFYVANIVFGNLSL
                     IATWLPIPVGALIPSLLCLIIVWWLLSKKRD"
     gene            complement(17382..18491)
                     /gene="lptF"
                     /locus_tag="PXE44_000893"
     CDS             complement(17382..18491)
                     /gene="lptF"
                     /locus_tag="PXE44_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDL5588420.1"
                     /translation="MILSRYLTKEIFKSQLAILFILLLIFFCQQLVRVLSSAVSGKVP
                     ADLVVSLLGLGMPTMAQLMLPLSLFIALLLTLGRFYAESEITVMRACGIGQGLLVKVA
                     MFLSIFTTALAVYNVFWLTPWAINKQSEMLAEAKANPRFAALSAGQFMSAGGYVLFID
                     KIENNSLNDIYVFQPEQQKKNKPSVVVAESGTLQGLPNGDQVLTLAESKRFEGTPQSA
                     DFRISHFENYSAYLGYQDVESDEKLVQRADFKKLMETKTPEAKAELHWRLALILAVPL
                     MALLAVPMSSVNPRQGRFAKLLPALLLYLIYFLLQSSLKSSGASGKLNAEVLMPLVSV
                     AFLVLGVILNAWDSKFMSKLRYQFGAKKLTNKAVA"
     gene            complement(18665..19462)
                     /locus_tag="PXE44_000894"
     CDS             complement(18665..19462)
                     /locus_tag="PXE44_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588421.1"
                     /translation="MRFWGKTFVVGLIGLGTQNTIFANDDAQISRYEQAKLRLSYDGN
                     VGNLLQQLSQRLKVGFIAYDVDITRKISLQNETETAIKTINEQIESQLTNIDVRFEKI
                     GERLFLVVSAKDVAPLLQNEPKEVQFVGDVIFDGEPNLTEQAQSTAEATKVQNILNIA
                     TNQEKIMAAKGKKAPQYKTVTKDNIGLKNIRVTALGTFLVFDTSVDAAKLRVKGNFED
                     IAQGENIIAILHQNTEAPPKIEIEDSQGKKLILEAHKATSSKKSKKQ"
BASE COUNT         5607 a         4453 c         3804 g         5718 t
ORIGIN      
        1 atgaggaacc aaactatggc taaaaatacg caaccatttg atgcccaagc agaagtgaat
       61 gagcttgttg aaaaaggctt aaaagccctt gatgagttta gaaaactcaa ccaagaacaa
      121 gtggatttta ttgtcgctaa agcctctgtt gctgcccttg ataaacacgg tattttagca
      181 atgcacgctt acgaagaaac cggacggggt gtgtttgaag acaaagccac taaaaacctg
      241 ttcgcctgtg agtatgttgt caataatatg cgacatttga aaaccgcagg ggtcattagt
      301 gaagatgatg tcaccggtat taccgaaatt gccgaccctg tcggtgttgt ctgcggtatc
      361 accccaacca ccaacccaac ctcaaccacc attttcaaag cgttaatcgc ccttaaaacc
      421 cgtaacccta tcgtttttgc tttccacccg tctgctcaac aatcttctgc ccacgctgct
      481 caaattgtgc gtgatgctgc cgtggctgcc ggtgcaccgg aaaactgtat tcaatggatt
      541 acccagcctt caatggaagg tacatctgct ttaatgaaac acccgggcat tgcaactatt
      601 ttagccaccg gcggtaatgc aatggttgaa gccgcttact cttgcggcaa accggcatta
      661 ggagttggtg caggcaacgt accggcttat gtagaaaaaa ccgctaaatt aaaacaagcg
      721 gtgtatgaca ttgtaatgtc aaaatctttc gacaacggta tgatttgtgc ctctgaacaa
      781 gcggcgattg tagataaaga aatttacgct gagtttatta aagagatgca atcttacggc
      841 gtctatttag tgaacaaaaa agagaaagcc ttattggaaa aatacatttt cggtattgat
      901 aaagccaaag atgaaaactg ctcaggtgca aaactcaatt cagcggtggt tggcaaaccg
      961 gctgcgtgga ttgcagaaca agccggcttt aaagtaccgg aaaaaaccaa tattctgtta
     1021 gccgaatgtg cctttgtggg cgaagctgag ccacttaccc gtgaaaaact ttcgcctgta
     1081 ctggctctgt taaaatccga ctccaccgaa cacggtttgg aattgtctga agcgatggtg
     1141 aatttccacg gtttagggca tagtgccgcc attcataccg aaaacaaagc cttagccaaa
     1201 acctttggcg aacgggtaaa agccattcgg gtgatttgga actcaccatc aacctttggc
     1261 ggtatcggtg atgtatataa ctccttcctg ccatcgctta cgctgggctg tggttcctac
     1321 ggtaaaaact ctgtaggtaa caacgttagt gcggtgaatt taatcaatat caaacgtgtg
     1381 ggcagacgga gaaataatat gcaatggttt aaagtgccat caaaaatcta ctttgagcgt
     1441 gactcaattc aatatttgaa atcaatgcac gatgtgcata aagtaatgat cgtaaccgac
     1501 cgctcaatgg ttgatctcgg ctttgtggat agaattaccg aacaacttcg ccaacgtcgc
     1561 aaccaagtga tgattcagtt ctttaccgat gttgaaccaa acccaagcct gcaaaccgta
     1621 caacgtggta cagaattaat gcgtagcttc cagcctgata ctatcatcgc acttggtggt
     1681 ggttcaccaa tggatgcagc caaaattatg tggttattct acgaacaacc ggaagtcgat
     1741 ttccgtgatt tagtgcaaaa atttatggat atccgcaaac gtgcattcaa attcccacaa
     1801 ttaggcagaa aagcgaaatt tgtcggcatt ccaaccacct ccggaacagg ctctgaagtc
     1861 acaccgtttg cagtaattac cgatggcgat attaaatatc cgctcgctga ctactcgctt
     1921 accccaacca ttgcgattgt cgatcctgcg ttagtaatgt ctgtgccgtc tcacgttgct
     1981 gccgacaccg gtttagacgt attaactcac gctaccgagg cttacacatc gattttagcc
     2041 aacgactata ccgacggctt agccttgcaa gccattaagt tagtgtttga aaacttggaa
     2101 aaatcggtca aagagtttga tgaaaccgcc cgtgaaaaaa tgcacaatgc ttccacaatg
     2161 gcagggatgg catttgccaa tgccttctta ggtatttgcc actcaatggc acacaaaatc
     2221 gggggtaaat tccacaccat ccacggtcga accaatgcga ttttactgcc acacgttatt
     2281 cgctataacg gcacacgccc aaccaaagtg gcaacttggc caaaatacac caattatgtc
     2341 gcagatgtgc gtttccaaga tattgccaga atgcttggct taccggcaga aaccccggaa
     2401 caagcggtgg aagcttacgc ccaagcggta cacgatttag ccgttcgttg cggcgtgaaa
     2461 atgtcgcttc gtgagcaagg cgttgatgag caagccttct tgaatgcccg ccgtgaactt
     2521 gccctacacg catttgaaga ccaatgcaca ccagcaaacc cacgtttggc aatgattgaa
     2581 gatatggaag cgattatgac taaagcctac tatggcaagg aataaagcac aatttaatcg
     2641 ttaaacttac taaatggccg cttgcaagcg gtcatttttc ttgaaatttt tacttttccc
     2701 ttcacaaaat actttcgctc ttttacaaag catagtattt ttatcgctcc ttttatctta
     2761 caggaaatac tatgcaaatt acgattttag acggcggaat tagccgtgaa ttaatgcgtc
     2821 gcaatgctcc atttcatcag cctgaatggt ctgctgccgc tttgtatgaa ggcccacatt
     2881 ttgtgcaagc ggtacacgaa gattttatcg ctcacggggc agaagtgatt actaccgaca
     2941 gctatgcggt tgtgccgttt cacattggcg aaaaacgctt tgcggcagac ggcaaaatgc
     3001 ttgccgattt agccggtcgc cttgccaaac aagcggtcaa aaattcagga aatattgcaa
     3061 ctcaaattgc aggctcactt ccaccaatgt ttggctctta ccgtgccgat ttaattcaaa
     3121 tggaacgctt tgctgaagtc gctcagcctt taattgacgg gctttcgcct tatgtggata
     3181 tttggctttg cgaaacccaa agtgcgatta ttgagccgat ttccatcaaa gcattattgc
     3241 cgaaagacaa ccgcccgctt tgggtctctt ttaccctcac cgatgatgaa ccaaccaccg
     3301 agccacaact tcgctcgggc gaaagcgtta aacaagcagt cgaagaaatg gtgaagttag
     3361 gcgtgcaagc gattttattt aactgctgtc agcctgaagt gatcggcgag gcattaagtg
     3421 taacccaaaa aactttagcc gaattaaaag ctacccatat tcgcacagga gcttatgcca
     3481 acgcctttgc tccacaacca aaagatgcca ccgccaatga cggtttagat gaagttcgcc
     3541 aagatttaac cctggaggct tatttaggct gggcaaaaaa atggacggaa caaggggcaa
     3601 ctattatcgg tggctgctgc ggcattgggg ttgagtatat tgaaacgctg gcaaaaaatt
     3661 taaaataagg aaatcgaatg tcaaataaaa aaataggatt aatttcactc accgccttag
     3721 tgctaagctc aatgattggc tcggggattt tcagcttacc gcaaaatatg gcagccgttg
     3781 ccggagcaga agccattacc attggctggc tgattaccgg tgtcggcatt atctttttag
     3841 gtttatcgtt tttctttatt tcccgcttgc gtccggattt agacggtggc atttacacct
     3901 acgcacggga gggttttggc gagctgatgg gctttatgtc agcgtggggc tattggctct
     3961 gtgccaccat tgggattgta ggatacctca ccgttgcttt tgaaggtttg ggtgtattta
     4021 ccgacaaccc gaacaccgtg attttcgggc aaggtaacac cctcgcctct tttattggat
     4081 cttcaatcat tgtgtggctg gttcacgccc tcatcgcagg cggtatcaaa gaagctgcct
     4141 ttgtaaactt aattgccact tttgtgaaag tcgctccgct cattctgttt atcgggttag
     4201 gcatttggta tttcgatgtg gatctgttta atagcgatgt taaagccacc gcacttaata
     4261 atagtgtaag cgaccaagtg aaaagcacaa tgttaatcac cttatgggta tttaccggtg
     4321 tggaaggggc ttctgtgctg tcggctcacg cgaaaaagcg ttctgacgtg ggactggcaa
     4381 ccgtattagg tattattatt gcacttgctc tttatgtggc aattaccatt ctttcactcg
     4441 gaattctgcc acgggaaact attgccaata tggcaaatcc gtcaatgggg ccgttgctcg
     4501 atgcaatgat gggcccaacc ggcaaagtga ttattactgc ttgtttaatt gtatcggtgc
     4561 ttgcctctta cattagctgg acgatgtatt cggcagaagt gccttatcgt ggctcaaaaa
     4621 acggggcttt cccgaaaatt ttagacaaag tgaataaaaa cggcacgccg attaactcac
     4681 tctggtttac cggctttatc gtgcaatttt gcttggtgtt ggtgtttgtt tttgagcaaa
     4741 gctataacac cttattgctg atttccacct cgatgatttt aattccctat ttcttaatcg
     4801 gggcttattt attcaaatta gcgattcaaa ccaacgccac ttggtacatt aaactcaccg
     4861 gctttatggc agccctttac ggattatgga ttgtctatgc cgcaggacta gaatatttgc
     4921 tgctttcggt ggtgctttat gtacccggaa ttttactctt cctctactcc aactataaat
     4981 tcaacggcag attaaccctt tcaaccgctg aaaaggtcat cttaatcgtg ctactggtaa
     5041 tttttggcta tgccgtgatt gagctgccag cactattagc agcataattc ccatcaaaaa
     5101 tgaccgcttg caagcggtta tttttctagg atcttctgca aagattcatt agaatagaaa
     5161 caacgtcaat aataaataaa aagaggagaa acaatgcaaa cttatcaaca taaagccctt
     5221 tgtcgggtaa gaaccattac cctttttctg agtttaacca aagataaatt gcagtgggaa
     5281 acggcattaa atgcggcaaa agcagaattt gatctattag ttcccgctat tcaaacacaa
     5341 ggctatcaca tacaatcgat tcgaatcgtt accaacagtt tcggggaata tcttgatctc
     5401 actaatattg aaacagcgaa acaggattta gcctatctta aacagctatt aacccaactt
     5461 aacgatagcg gtttacgcat tcgctttgcc attggtgagg caaaaacacc ccacgaatta
     5521 gcattactac ctgagctgat tttaaattat ggtgatttat ctaacgcttg tgtcaatatt
     5581 gaacccgatg aatttgggat tttagacaat gaaaccattt tacaatctgc tcaaattatc
     5641 aaacaaatcg ctgaaattac cccaagaggt gaaggcaatt ttaattttac ggtgaatttt
     5701 aactgtaaac cttttattcc ttattttccg gcaagctacc attcaagcaa gctccctaac
     5761 agttttgtga taggcctaga aacaccagat ctattagtag cggtgttaaa agagctgaaa
     5821 gccagcccac ataacgactt ttatcgccaa tgctatgaaa cgatgtctca agccttgcaa
     5881 tatcacgttg atgaagttct cgaagcagta aaatcggtac aacacaaatt acaatttgcc
     5941 tttgctggca ttgatagttc tgctgctccg tcaaaaaact gtgcctcaat gacagaagtt
     6001 tatgaattaa tgggcgtacc attttttggg gcagccggaa cagtagaagc ctcttcactt
     6061 ctgacaaaag tctttaaatc tatcaaacat gtaccgcttg tcggtttttc aggtttaatg
     6121 ttggcagtaa ccgaagattt aggcttagcc gcaggcacac acaaagccca attcgacatt
     6181 cgtgctttac taacttatag tgctgtttgt ggcattgggt tagataccgt accgatttcc
     6241 aatgaagcca ctgttgaaca aattgccgca ttaatgcgag acaccggcac aatggcattt
     6301 agactaaata aaccgcttac agtacgttta tttccaattc cgaataagag tgctggggaa
     6361 gtgacagaat ttgaaagtga tgatttatgt aactgccgaa ttttagctgt accttaaaat
     6421 aaaggctgat ggatatttcc atcagccttt caatttactt caccttcaat tttttaccta
     6481 ccgtgatctg ataatttttc aaattattca gctcactgat tttttgcggc gtagtaccgt
     6541 aagctctagc aatcgagtaa accgtttcat cttgttggac aatatggtag ccactgttag
     6601 acaccttaat ttctctttga gagtctgatt ttttagactg atttttttga tctttctcaa
     6661 gagctgtttt ttcttttagt tgctgctctt ttttcttatt ggttttagcc gaatcttctt
     6721 ttttatcctc ttttttgctc ttttctttca attttgtctt atctgattgc gtattcttct
     6781 cttgtttctc agaagcaggc tttttagtta aatcagcttt agcagttttt tcctctttct
     6841 tattttgctt atcttctttg tccgattttt tactgaccga agaactagca actgcatttt
     6901 ttgcccgata agcgactaat ccattataaa tggcccgagc aacttgttta cgataatttg
     6961 cactggctag ctttgcttcc tcggttgaat tagataaaaa tcccgtttcc accaatacag
     7021 atggaatatc aggtgaacgc agaacactta agcttgcgtg ctgaggagac gattttgcta
     7081 atgcagccac atttgacatt ttagctaaaa tgctcttgcc taattcatag ccggcacgtt
     7141 gagagtgaga gaactgtaaa tcaagcactg tttgatttaa ataacgttca ttgttgcttg
     7201 aaagcaccga accagccccg ccaagtaatt ccgactgctt ctcgtggtct tccaaccatt
     7261 tacccatttc atcgctagca cggctattag aaagtaccca aaccgaagcc cctttcaagg
     7321 aatcgccacg aggcgaagag tctgcgtgaa tagaaaccag cagatcggca cgatgcttac
     7381 gggcaatctc ggtacgttgc ggtaattgga taaaataatc tcccgagcgt gtcatcactg
     7441 ctttaaaatt aggatcggca tctaacaagg cttttagctc tttagaaatt gcgagggtaa
     7501 cgcttttttc caaaatcccc agatttttac caatagcccc cggatctttt cccccgtgtc
     7561 cggcatcaat cgccacaatg gttttggcaa atgtggaaaa actgaataaa ctcaatgtgc
     7621 taaaaacaat aaatctgata acttgcttca ttaccttccc tacaagcggt ctatttttga
     7681 tgaaaattta ctaatatatc tactgcagtt tgattttgcg gcaacaggct aatctgccgt
     7741 ccgtcattct tataatcaat ttggatcaca aaatcagcct ctggaatcat tcctttacct
     7801 ttaactgccc attcaagcaa acaaagcgtt tgtggtttga aataatcacg aattcccata
     7861 aactccagct cttcaggatc ggctaagcga tacaaatcaa aatggtaaag gctgaaaggc
     7921 ggaagttggt attcttccac cagagtataa gttgggcttt ttacattgcc ttgatgcccg
     7981 aaagcccgta cgatcgagcg ggttaaggtg gttttacctg ccccaagttc gccgtttagg
     8041 taaacaacca gcgaatgagc ttggtcttgc aataaatatt tcttaagttc taacgcgaat
     8101 gattgaccaa attccagcaa tttagtttca tcttcgcagt aaaaagagag agaatctgtc
     8161 attatttttg gtctagctga ataaaatgtt cccgataaaa tttcaattcg ttaatggatt
     8221 cacgaatatc gtccaacgct aaatgcgtat tgcctttact gaatttatct aaaatctccg
     8281 gtttccaacg gcgtgccagc tcttttaaag tactcacatc taagtggcga taatggaaat
     8341 aatcggctaa atcaggcata tatttataca aaaaacgttt atcttgagca atgctgttgc
     8401 cgcaaatcgg agaagcgcct tttggcaccc agcgttttaa gaagtctaaa gtttgcaact
     8461 ctgctgcccg ttcagtcaat ttgctcgctt tcactcgttc aatcagcccg ttagcggtat
     8521 gggttttgac gcaccaatca ctcattttct ctaataaatc atcggtttgg tgtaccgcaa
     8581 gtaccggtcc ttccgctaaa atatttaaat ccttatcggt cacaatggtg gcgatttcaa
     8641 taatgcgttc tttttccgga tctaaccctg tcatttcaag atcgatccaa attaaatttt
     8701 gttcgttttg agccatttta tttccttaca cttttgcaat ttttagggca tttttgaccg
     8761 cttgcggcac aaattggctg atgtcaccat tgtggcgata gatttccctt accatcgttg
     8821 aagaaaggta acgccattct attgaagggg gaaaaaaaat agtttctaaa tcgcccgata
     8881 atttgtaatt taactgagcg agttggattt catagtcaat atcatctgcc ccacgaatac
     8941 cacggattaa cgctttggca ttctgctgtt tggcaaagtc tgctaacaat ccactaaagc
     9001 caatcacctc aatattttgc cactgggtac agctttgttt aacaagctcc actcgttctt
     9061 caagactaaa aagcggctgc ttgcttgggt tttgagcaac ggcaacaatc acttttccaa
     9121 ataagtgaga ggctttgcaa attatgtcca aatgcccatt ggtaatggga tcaaaggtgc
     9181 ctgcataaat tacgttataa ctcattttat ttctctaaat aggaggctaa taaatccaaa
     9241 tggcgtttta atgccccttg attttcctgt aacacgctta agccggattt tgcaatttct
     9301 tgtccgattt ggggctgctt aagcaataat tgaatgctct cggtcaaagc ctcgactgaa
     9361 ctttcaattt caatcacgcc tttcacctca cgcaatttct caaagatttc agggaaatta
     9421 taggtgtaaa gcccagaaac caccggaata ttaaaagcga ttggttcaag tggattatgc
     9481 ccaccgtgtt tgaccaaact gcctcctaca aaagcaatgt cagacaaccc atacagtaac
     9541 atcatttccc ccattgtatc acctaacatt acttgagtat tttggcttaa cgttttattc
     9601 tcagtacgtt ttacataatt taaatctgat ttttgaatca gtagctcaac taaaccaaaa
     9661 cgctccggat ggcgaggcac taaaatcaac actaaattcg ggtaatgctc taataagagt
     9721 ttatgagcat ctaaaatcag cttctcttcc ccttcgtgag tactgccggc aatccaaatc
     9781 ggtctctctg ttaaattcag actagcttta gttttaatca ccgattgatg aagctgtggc
     9841 gtaatttcta aatcaaattt caggttaccg gtattaacca tttttgccgg tgcataaccg
     9901 agtgctaaat agcgatctcg gcttacctca tcttgagcca taatcagact aatttggttg
     9961 agcatatcct taatgctacc ttttacccag ccataacgct tcgcagaacg tggcgataag
    10021 cgggcattgg caatcacaaa tggaatttta cgaatattta ctttacgaat taaattcggc
    10081 caaagttcgg tttcaattac aatcatcaat ttcggatcga taaaattcaa aaaccgctcg
    10141 atagcatcag gtaagtcata aggcaaataa aaatgtgaaa cactattacc aaaagcagct
    10201 tgcacacgcc cagagcctgt tggcgttacc gttgtgacta tcaacggaag ttcaggatat
    10261 tgcttgctga ctgccttaat caaaagcgtt gcagcaatca cctcaccaac cgatgccgca
    10321 tggattacca ccccttttgc tttcggtttt tccgattcgt cataaatccc ataacgctcc
    10381 cataaacgtt tccgataagc cggttgttta cgccctttgt accacattaa tagcagaacc
    10441 acaggctgta atagataact taaacaaata tagagtaaac gtagcattta ttttccttat
    10501 accataaccc tcacctcttg tgggagagag tcaaatacgc caataaatcg ttcctctctt
    10561 gttcgagagg ggggaacaag cggttatttt taccaaaaat tttgcaatct atatcctcaa
    10621 agacaaaacc acggaataac cttaatccgt ggtttatctc gcatcattac gcttgatgtg
    10681 gattgcgggt attcgtattc actaagttac gcattaaaag tgcaaaatcc aactcaatat
    10741 ctgccggcac atctaaaaag acaaaatgcc cgtcaccgag tgctgcgtcc acattttcat
    10801 ttttgcggtt aagcatacgc tcgattttaa atacaatgtt gcctttaggg gtcatcattt
    10861 ccactgaatc gcccactaag aatttgtttt tcactgccac ttccgccaag ccttctgcat
    10921 tgcgtttgcc ggtaaattcg cccacaaatt gttggcgttc tgaaatcgaa tagccatatt
    10981 catagttttg gtattcatcg tgagtatgac gacgtaagaa gccctctgta taaccacggt
    11041 gtgcaaggct ttcaagagta tccattaaac tttcgtcaaa cggtttaccg gcagcggcat
    11101 catcaatcgc cttacgatac acctgagccg ttctcgcaca gtaatagaac gatttcgtac
    11161 gcccctcaat tttcagtgaa tgcaccccca tttgagttaa acgctcaacg tgttgtactg
    11221 cacgcagatc tttagagttc ataaaatagg tgccgtgttc atcttcaaag gcggtcattt
    11281 gctcatcagg gcgttgtgat tctgtataaa ggaacacttt atctgttgct tcgccattac
    11341 ctagagtagg agccacattt ttgacttcaa tttccggctc atacttaggc acaatttgcc
    11401 ctacctcatc aatcttacct tcttccattt tatattccca acggcaagca ttggtgcaag
    11461 tgccttggtt tgggtcacgc ttattgatat agcctgaaag caagcagcga cctgaatatg
    11521 ccatacacaa agcaccgtga acgaagatct caagctccat atccggcact tgttcacgaa
    11581 tttcttcgat ctcttcgagt gataattcac gggataaaat cacacgggtt agccccattt
    11641 gacgccagaa tttcaccgtt gcccagttta ccgcattggc ttgcaccgaa agatgaatat
    11701 ccatttcagg gaaattttca cgcaccaaca taattaagcc cggatcagac ataatcaaag
    11761 cgtccggctt catatccact accactttta gatctttgat gaaggttttt aattttgagt
    11821 tgtgtggcgc aatgtttacc accacataga atttttttcc taaggcgtgg gcttcatcaa
    11881 tcgcaacttt taaattggcg tggttaaatt cattattacg cacacgcaag ctataacggg
    11941 gctgtcctgc ataaatcgca tcggcaccat aagcaaaagc gtaacgcata ttttttaaag
    12001 aacctgcagg cgaaagtagc tcaggtttgg catattttaa agtcatatta ttctcttttc
    12061 tgataacaag tcaggacttc tcgacgtgag aagcgtaagc ggtatatttt ctaagaaata
    12121 ttgcgtgatg gcaagctaat ccattacaat ccgattttta tgtggggaac aatatgaaac
    12181 ttaaatacct tttagcaaca acattactgg ctttttcaac atttaccgcc gctcaaagca
    12241 tcaagacgga gatgatgcaa atgaatatgc aagcgaataa attgatgcgt gccgaatcgg
    12301 cagaagagtt tagcgaaatt gcgaatgatt tcattataat taccgagaaa accaaaggga
    12361 tttttcccaa tagcgtagaa ggcgataaag cagctcagga agattaccaa gccggtatgc
    12421 aaaaattaat tgatatggtt aatcaagcca gtgagaaagc tcaagcaggt gaattgcaag
    12481 aagcaaaaat gctgatttcc gagcttgaaa tgatcaagcg tgaatatcac aaaaaataca
    12541 aataattcaa cacattaaaa gggtgagcta aagctcaccc tttctattca aattccagtt
    12601 caaccgaatt ctcaccaaga agtgccttta attcgctaaa cattgcctca ttcgggctga
    12661 ttcgccactc tactccgcct ttgagcaata ccctaccttc cgggctttgg taataaaaat
    12721 gcagcggtaa agtgccttct ttatgtgggg tgatgatagc ggtcattttt ttaataaaat
    12781 ctgcagaaag ttgctctttg ctaatcgcta acgctaaact tttggcaaat cgaccccttg
    12841 cttcatcaag ggataacact tctcgagccg acattttcag tccaccgctg aaatcatcaa
    12901 actgtaccga acctgttgcc acaataattt ggtcttgttg catcaaatgc ccgaaatttt
    12961 ctaaggcttc cgagaaaagg gtaatgtcca atttccccga gcgatcttca atagtcgcaa
    13021 tcccaatacg gcttcctcgc ttggtaactg ccactcgaga tgccataaca attcctgaaa
    13081 ccgtggtcat ttgccctcgg aaagtcggtt gcaattcatt cagccgatta ggggcatagt
    13141 gggataattc tttgaggaat ctaccaatcg ggtgtccgct taaatacagc ccgagcgtgg
    13201 cacgctctcc atctaaaatc acttgctccg accattttgg cgtattggcg taagcatttt
    13261 gcacttcttc cggtgtctcg gtaagcacgc caaacatatc gctctgcccg agttcctcca
    13321 ttttagcgtg ctgatctgag gctttaagtg catcttccaa attcttcatc aaggcagctc
    13381 ggtgagggcc taatttgtca aaagcaccgg acataatcag gctttcaaag gtgcggcggt
    13441 tgattttttt cagatcgacc cgagcggtta aatcaaatag atctttaaat ttcccctctt
    13501 tttggcgagc tgccagaatt gcctcaatcg gtccttcccc aacgccttta attgctccta
    13561 gcccgtaaac aatttcgcct ttttcattta cgctaaaacg atgtttaccg ctgtttacat
    13621 caggcggcac aacggttaag cccatattca tacactcatc gtagaacccg acaattttat
    13681 cggtattatc catctctgag gtcattaccg cagccataaa ttctgccgga taatgggctt
    13741 ttagccaaag tgtttggtaa gaaaccaacg cataagctgc tgagtgagat ttattaaagc
    13801 cgtagccggc aaacttttct accaagtcaa aaatttgacc ggcaagttta ccgtcaatcc
    13861 ctttggcttc cgcccctttt tcaaacactt cacgctgtgc agccatttcc tccggtttct
    13921 tcttacccat tgcacggcgg agtaagtccg ctccaccgag tgtataaccg gcaagctctt
    13981 gtgcaatttg cataacctgt tcttggtaaa caatgacccc ataagtcggc tcgagaatag
    14041 gttttaagca ttcgtgttgg tattgcggat caggataaga aacctcttct ttgccgtgtt
    14101 tacggtcaat aaagttctgc accatacctg attccagcgg ccctgggcgg aatagtgcca
    14161 ccaatgcgat aatatcttca aaacagtccg gcttcaggcg agaaattaag tctttcattc
    14221 cccgtgattc aagctggaat actgctgtag ttttagctgc cagcaacaga gcaaaggatt
    14281 ttttatcatc aagcggaatg gtttcaatcc gaatcggctc tttgccttca cgagccaaac
    14341 gttcgttaat catctccaac gcccatttga tgatggtgag cgtacgcaag cctaagaagt
    14401 caaacttcac caatcctgca tattccacgt catttttatc aaaatgggta accgggtgta
    14461 agccctcgct atcgcaataa agcggcgaaa aatcggtaat cgcagttggg gcgatcacca
    14521 caccaccagc gtgtttacct gcattacgag ttacaccttc gagcttacga gccatatcaa
    14581 taaggtcttt cacctcttca tcggcttcat atagctcagg caattttggc tcagcggcaa
    14641 aggctttttc aagcgtcatt cctggatcgg gcggaatgag tttggagatg cgatccacaa
    14701 agctataggg gtgtccaagc actcgcccta catctcgaat gaccgctttc gccgccatag
    14761 taccgaatgt gataatttgc gataccgctt gtcgcccgta ggtttccgca acgtgttcaa
    14821 tcacacggtc tcgcccgtcc atacagaaat caacgtcaaa atcgggcatt gaaacacgct
    14881 cagggttgag aaaacgctca aaaagcaaat caaattccaa tgggtcaaga tcggtaatct
    14941 tcaacgcata agccaccaat gagcctgccc ctgagccacg acccggtcct accggaatat
    15001 cattatcttt ggaccattgg ataaactcca tcacgatcaa aaagtaaccc gggaacccca
    15061 tttggttgat cacatctaat tcaacttgca atctttcgtc ataaaccgac cgcttgttcg
    15121 ccctttcctc aggatcaggg aatagaaact ctaaacgctc ttctaagcct tctttcgatt
    15181 tttttaccaa gaaatcttcg gtggaaagct caccggtcgg gaaatttggc aggaaatact
    15241 cgcccaaacg cacggtaacc gaacagcgtt tcgcaatttc aaccgtgttt gccaatgcac
    15301 tcggtaaatc ggcaaacagc ttgcacattt cctcttcagt acggaaatat tgtttcggtg
    15361 agtatttttt cgggcgtttc gggtcgtcta gggtgtagct gtcgtgaatg gcaacccgaa
    15421 tttcgtgtgc gtcaaagtca tcttctttca agaagacaac atcatttacc gccactaacg
    15481 gaattgcatt tttttgtgaa aattcgaccg cttgtttgat atagcgttct tcgtctgccc
    15541 gtccggtacg gcaaacggaa aggtaaaaat gattcgggta atattgctga taaaaagcta
    15601 atttttcttc agcttcttgt cggttttctt tgagtaaggc tttacccaca tcgcccattc
    15661 gcccgccgga aagaatgatg atgccctcat tcagctctgc taaccattca cgttccacca
    15721 tcggaaattc cacatagcct tgctgatagg ctttggacaa aatttgggta atattatggt
    15781 agcctttgtt gttcttcgcc agtaatgtca gctcaaaata atcttcattg ccgctctcgg
    15841 ttctaacata aacatctgcc ccgataatcg gcttaatccc tgctcccaat gcttcgccat
    15901 agaatttcac caacccgcaa aagtttataa aatccgttaa gcccattgcc accatattat
    15961 tagcggcagc ggttttaact aaggatttta ccttagctaa accattaatc attgagaaat
    16021 cactgtgaac ttttaaatga acgaaacgac aatctgacat aggaatataa tctaagtaaa
    16081 gaagtttccc ctctttagta aagactgtca ggttctaaaa agtgaagcct tctgtgaata
    16141 atcccgttgg gcgaatgtta ttcgccccta caaacgagca ttattcttgc tcagagttac
    16201 atttaaaatc cgaatgtagg ggcgaattac attcgccctt tcagaaccta acaaccctgc
    16261 tttatctaaa gaagggagtt aaaattaatc acgttttttg cttaacagcc accaaacgat
    16321 gatcaaacag agtaggcttg gaattaatgc tccaaccggt ataggtaacc aagtggcaat
    16381 taagcttaaa ttgccaaaca caatatttgc cacatagaac ataaagccgg caacaatacc
    16441 aatcacaatt ttcgccccca tcgtactact acgaagcggc ccgaaaataa aggagatcgc
    16501 aagtagcatc atcaccgcca ttgagattgg ttggaatact tttcgccaga aggtgatctc
    16561 aaaacgtttc ggatcttgtc cggtatcttt taagaagccg acataatctg ccaagcctga
    16621 aatcgagagc gactccggtt tgagtgaaac aatgcctaat ttgctagggg ttaaactggt
    16681 ttgccaaact tgatctggct gtttagcttg ctggatctga ttttctccca cagtcgattt
    16741 ttccacttta gatagcaccc agcctttttc atcactgtaa ttcgcagttc gggcttttaa
    16801 aacagattgc aattcacgtt tgttttgtcc ttcgccgaat tgataaatca gtacgttatc
    16861 taagcttttt tcatttttaa tatgattaat atagataaat gaattgccat ctttcgccca
    16921 aaacccgtct tttgtggcaa gcattgagcc gccactttgg gcaatcgaac gcatattacg
    16981 ggcaaactgc tcggtttgtg gcacgcccca ttcgccgata atcatcgtaa aaatgactaa
    17041 cggtaaggca gttttcatca cggctaaacc aatgcggaaa cgggaaaaac cggaggcttg
    17101 catcaccacc aattcactaa ggctcgctag gttgcctaaa ccgagtaatg agccaagtaa
    17161 tgccgcaatc gggaaaaagg tttcgatatc tcgtggaata gttaaaaaag tatagtatgc
    17221 cgcttttaag ccatcgtaag atccacgtcc tacatcacga aactcttcca caaatttaat
    17281 aatcgcccca agcccaatca acataaacag ggttaaaaaa atcgcggata aaatcgtttt
    17341 gccgatgtag cgttctaaaa tattcattga gaaaaattgc attatgccac cgccttattg
    17401 gttaattttt ttgcaccaaa ttgatagcgt aacttagaca taaatttact atcccacgca
    17461 tttaaaataa cacctaaaac taaaaaggca actgatacca acggcattaa cacttccgca
    17521 ttcaacttgc ctgatgcccc tgacgatttt agcgagcttt gtagtaagaa atagattaaa
    17581 taaagcaata acgccggcag tagtttagca aaacgccctt gacgagggtt tacactactc
    17641 attggcaccg ctaataatgc cattaacggt acagctaaaa tcaaagcaag ccgccaatga
    17701 agctcggctt ttgcttccgg cgttttggtt tccattaatt tcttgaaatc ggctcgttgc
    17761 actaatttct catcagattc aacgtcttga tagcctaaat aagcagaata attctcaaaa
    17821 tgtgaaatac ggaaatcggc actttgtggt gtaccttcaa aacgcttgct ctctgccaag
    17881 gttaagactt gatccccatt tggcaaacct tgcaacgtgc cggattcagc aacaaccact
    17941 gaaggtttgt ttttcttctg ctgttccggc tggaaaacgt agatatcatt tagactgtta
    18001 ttttcaattt tatcaataaa taacacatag ccaccggcag acataaattg tccggccgaa
    18061 agagccgcaa aacgtgggtt ggctttcgct tccgctaaca tttcactctg tttattaatt
    18121 gcccaaggcg taagccaaaa cacgttataa accgctaatg cagtggtaaa aatcgagaga
    18181 aacatcgcca ctttaaccaa tagcccttgc ccgataccac aagcccgcat tacggtaatt
    18241 tcactttcgg cataaaaccg cccgagggtt aataacaagg cgataaacag tgagagtggc
    18301 agcattaatt gtgccattgt cggcatacct aagcctaata agctcaccac taaatctgcc
    18361 ggtactttac cactcacagc agaacttaga acacgcacta attgctgaca aaaaaagatc
    18421 agtaataaga taaacaatat cgccaattgg cttttaaata tctctttggt taaatatcgg
    18481 cttaagatca cgttttttct ctataacatt tacaaatctg aataccgcta aacacaagcg
    18541 gtctattttt gttaattttt tgcaaagagc gcaaaataac ttattcgaat cgctaataat
    18601 acctttttca tacaaaaaaa tcacctatag aaaatatcac gcacggcaaa accgtgcgta
    18661 agtattattg ttttttcgat tttttcgatg atgttgcttt atgagcttca agaattaatt
    18721 tcttaccttg gctatcttca atttcaatct taggtggtgc ttccgtattt tgatgaagga
    18781 tagctataat attttcacct tgtgcaatat cttcaaagtt tcctttcaca cgtaacttag
    18841 ccgcatcaac actagtgtca aatactaaga atgtacctaa tgcggtaact ctaatatttt
    18901 taagccctat attgtccttc gttaccgttt tatattgagg tgcttttttc ccttttgcag
    18961 ccataatctt ctcttgatta gtcgcaatat taagaatatt ttgaacttta gtcgcttctg
    19021 ctgtagattg tgcctgttct gttaaatttg gttctccatc aaaaataaca tccccaacaa
    19081 attgcacctc tttaggctca ttttgcaata atggtgcaac gtctttagct gaaacaacca
    19141 aaaataaccg ttcacctatt ttttcaaaac ggacatcaat attggttaat tgtgattcaa
    19201 tttgttcatt aattgtcttg atcgcagttt ctgtctcatt ttgaagcgat attttacgtg
    19261 taatatcaac atcataagca ataaaaccaa cttttaaacg ttgtgataac tgctgaagta
    19321 aattgcccac atttccatca taagaaagac gtaactttgc ctgctcatag cgactaattt
    19381 gagcatcatc attagcgaaa atagtgtttt gagtccctaa gccgattaga ccaacaacaa
    19441 acgttttacc ccaaaagcgc atcattttta tctccaatta ttctcttaaa tctaaaaaac
    19501 tcttttagta taaagtaagc aaactaaaag aaaaaccagt aaaattatct atacgaccgc
    19561 aaaaaacatt taatatttgc aa
//
LOCUS       DANLZC010000032        19356 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.032, whole genome shotgun sequence.
ACCESSION   DANLZC010000032 DANLZC010000000
VERSION     DANLZC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19356
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.032"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            25..1143
                     /gene="rnd"
                     /locus_tag="PXE44_000895"
     CDS             25..1143
                     /gene="rnd"
                     /locus_tag="PXE44_000895"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDL5588422.1"
                     /translation="MESLIHYQWVETNEQLEAVCEQARKTKVVALDTEFIRTRTYYPI
                     LGLIQLFDGQQVSLIDPNTISDFSPFVSLLADKNIIKVLHACSEDLEVFEYQFKQLPE
                     PMLDTQVMAAFAGIGISMGFAKLVSHYLNIELDKGASRTDWLARPLSAEQLQYAAADV
                     WYLLPVYEKLAQALTKSTWQSAVAEECVALLKKAKQPINKEKAYKNIANAWRLNREEL
                     AILQVLAKWRIEEAQKRNLALNFVVKEASLVQIAKSCPKHTSSLLEFMHPNEVRIHGK
                     KILWLVEQGKAIESNDYPELIQRLVDEKGYKYYMQAMLQKLLEIQPLDIVPELVASKR
                     QLNQLFKWYIDGKPSEKIPELLIGWRKPFGEQLLSVLS"
     gene            1200..1682
                     /gene="lrp"
                     /locus_tag="PXE44_000896"
     CDS             1200..1682
                     /gene="lrp"
                     /locus_tag="PXE44_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005695827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDL5588423.1"
                     /translation="MENKKPPKALDAIDLKILNELQRNGKISNIELSKRVGLSPTPCL
                     ERVKRLEKNNVIMGYKALLNPELLDAPLLVIVEITLVRGKPDVFEEFNKAIQQLDEIQ
                     ECHLVSGDFDYLLKTRVADMVSYRKLLGTTLLRLPGVNDTRTYVVMEEVKQTNYLLLK
                     "
     gene            1693..4353
                     /locus_tag="PXE44_000897"
     CDS             1693..4353
                     /locus_tag="PXE44_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HDL5588424.1"
                     /translation="MIEQLKPHLKGKENLIKLMLLLTGFLGVYLLIAWASYSPLDNAW
                     SVASSITQTTLNKTGHFGAWSIDMLYAMFGKVAVLVPIALIIFSIYSLGMGLSVDMKW
                     KTVFIRLASFLLLMVGLAGLFSVLFSNSAYYLSGGFVGGVWETILSDTIGQFGALLIA
                     MLFTIAGLYFCSVQSLLPILTQFYEWIMAKSEESKTAESLQHLQNTEQKQPLVTENEA
                     VEVVDDHKPQFTDISAFKRPNISGLRHSENVEIDNREMYEIERDLELPTINILIQPDM
                     VNQVAEQATSGYELPNEESNVLQMPTIRLNTAIENEVDTIENSSSLEDLKIAESMLPQ
                     IQLDNDIEDTEEAIDDVREITPEFMVTPPKAMKDVSEQPAYPKGYGDTLIHPLLQRKV
                     ITEKPTTPLPTLDLLDKAPIQSQQITEQEIRETSVRLEAELANFGVKATVEDVLVGPV
                     VTRYEIQPAAGVKASKITNLASDIARGLMFKAIRITEVIPNKPYMGIETPNKHRETVW
                     LRDVLDSDEFRNTTATLPMALGKDISGEPVVVDMAKMPHLLVAGQTGGGKSVGVNTMI
                     LSLLFKLTPEQVRFIMIDPKVVELSIYNDIPHLLTPVVTDMKKAANALRWAVEEMERR
                     YLLVSHLQVRNIEGYNAKIDQAAAMNLPIPDPTWRPGDSMDSLPPPLQKLSYIVLIVD
                     EFADLMMSAGKEVEEYIMRIAQKARAVGIHLILATQRPSTDVITGVIKANIPSRIAFT
                     VASQIDSRTILDSGGAEALLGRGDMLYSGAGSPDIIRIHGAFMKDEEVQRVADNWRAR
                     GKPNYLESIVESRSEEADGKNDGGTGDLDPLFDEVVEYITETGSVSISNIQRRFSLGF
                     NRAARIVDQMEAQGIVSEPLKGGKREVLAR"
     gene            4505..5344
                     /locus_tag="PXE44_000898"
     CDS             4505..5344
                     /locus_tag="PXE44_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDL5588425.1"
                     /translation="MRLDDIKESKNVEDRRFNNVGRTKVGRGKGGILTFIIVLVGAYY
                     GVDLTGLMGGGVEYEEQTSALNESEEKQLRTLSAKVLTTTENIWTSYFQQNGLTYKEP
                     SLVLYRGAIQTACGTGQSAMGPFYCPVDQKVYLDLSFYDDMRKKLRASGDFAFSYVIA
                     HEVGHHVQNLLGITSKTQRAQMKASSKAEANQISVNVELQADCFAGVWGHQLAKANRL
                     EEGDVEEAFNAAQAVGDDRLQQQSRGYVVPDSFTHGSSAQRLAWFRKGLTTGNPAVCN
                     TFN"
     gene            5433..6515
                     /gene="aroG"
                     /locus_tag="PXE44_000899"
     CDS             5433..6515
                     /gene="aroG"
                     /locus_tag="PXE44_000899"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDL5588426.1"
                     /translation="MSYIKNDDVRITSIEELLPPIALLEAYPASDVAAETVAKTRQSI
                     HKILHGADDRLLVVIGPCSIHDPKAALEYAQKIKAMRADPKINQNLEVVMRVYFEKPR
                     TTVGWKGLINDPYLNETFALNDGLRIARKVLSDINDLSVPTAGEFLDMITPQYVADFM
                     SWGAIGARTTESQVHRELASGLSCAVGFKNGTNGSVKIALDAISSAKSPHHFLSVTKF
                     GHSAIVSTAGNPDCHIILRGGDSATNYDADSVAAACAAIEKSGGRPHVMIDFSHANSQ
                     KQFKRQMDVCADVCNQIAGGSDFISGVMIESHLVEGRQDLIEGKGLEALVYGQSITDG
                     CIGWEDSEKALFALAEAVEKRRNFAK"
     gene            6602..7294
                     /gene="tsaB"
                     /locus_tag="PXE44_000900"
     CDS             6602..7294
                     /gene="tsaB"
                     /locus_tag="PXE44_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL5588427.1"
                     /translation="MKPSILALDTATEACSVALQYNGNITFLDEISPRSHTQRILPMV
                     DELLNQANIELKEVNYLVFGRGPGSFTGVRVGVSVAQGLAMGANLPVVAVSNLKAMAE
                     EAYQKLGATKVIALIDARMNEVYFAQFERKGEEWNEVVAEQVCSPEVAISQFQMSEET
                     VVVGTGWAAYSQFSEQNLPLVISDITLPSSRYMLSIAETEIQKGNIQSAFEIEPVYLR
                     NEVTWEKLPHKQ"
     gene            complement(7311..8120)
                     /locus_tag="PXE44_000901"
     CDS             complement(7311..8120)
                     /locus_tag="PXE44_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL5588428.1"
                     /translation="MTMTVIIILIICGIIVNITSALFGIGGGVLMMPILHSLFPELPL
                     QVISACSLTIVIGSATINLCLFHHQQIKINRKNVLIWSLAMMLGVQIGFELSFLFSDN
                     SIIAIFILTLLTLSFKTLFHKPRVKAIVPPKPKEAVSNATMCSLGGLIAGITGIGGGS
                     IMAPIVTQIRSVSPKQVAVYTNYMMVIGGIGNLYGYLSRPFVLNSTLATWQIGYVNFA
                     IVGTVVISSLFTVYFSMKLRNILSPELTTKLLGVILFTIACYMGFLQYLFT"
     gene            8219..8770
                     /locus_tag="PXE44_000902"
     CDS             8219..8770
                     /locus_tag="PXE44_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL5588429.1"
                     /translation="MNNMPTPEQLMSCQLSVMSDLEKGVVIYSQGYHAREFYFLKSGL
                     IGLYHMLENGKESLVRVYKANDYFGFRTFFGDNRYHCSARVLQPAELIRITPLNFIEF
                     WSNNSALIISLLKQLSTELQDAEHRLSMVSYQKTQERVMNSIHYLHQRYPNYHWTNRE
                     IAEFAGCETETAIRLRRELKKIS"
     gene            8914..10611
                     /locus_tag="PXE44_000903"
     CDS             8914..10611
                     /locus_tag="PXE44_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL5588430.1"
                     /translation="MLLTIITFLVVTGLVGYISWLKTRNDDLSTSKGYFLAGRGLSAT
                     VIGCSMVLTSLSTEQLIGVNANSYAGNFSIIAWTVPAVIPLCFLALYLLPKYIRNGYT
                     TIPEFFENRFDRQTRLIMSGLFLVFYLFIVIPTALYTGAIAFNKIFNLETAFGLSYGA
                     AITYTVIAIGIVGAIYAIFGGLKAVAVSDTWNAVLLVIGALLVPVFALIYLGNGSMAE
                     GVKIITTTHVEKWNAVGSATDAVPWPTIFTGIMVVHFFYWTTNQAIVQRALGARDLKA
                     GQKGILIAALFLLLLPLILNLPGLLSFHILGEGLKPIDVSYPELVNKTFPTVLQGFFI
                     AALFGAILSTFNSFLNSAATIYCKDLLPSISKKQFTDAELISYAKKVSAVMAVITMIF
                     GPLLMFGTDGIFLLTKRFAGFVNIPIVALFVIGLFNKTVSGKAARIALLAHVILYFCI
                     VWVFDVKINFVYVQAGLFVFDVALMLILGIFLKRATPYEDNKINHANVDLTSWEYAPL
                     AVASIVLGLFTLYAFLSPLGLASPDGNPAMVLAVYAVLQAVVCVVFFAKCKGKKAVN"
     gene            10758..12206
                     /locus_tag="PXE44_000904"
     CDS             10758..12206
                     /locus_tag="PXE44_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL5588431.1"
                     /translation="MNNIIYILLDQVRKDMLGTYGHQVVKTPNLDRLAKDGIRFNNAF
                     TPASVCGPARTSLFTGLMPSSHGIIKNGEKGGTGEIPQQNPNIAGVEGYNSFVIGKWH
                     VGTKSVPEDYGIAGHNFDGYGYPGSQVYKNLVFNQPPTHSNRYREWLAEKGYDAPEVS
                     RAYFGDNPHLRVQELCGLLSGSREQTIEWFIIDEAKKYIQASLDEKKPFFAWINFWGP
                     HTPCVVPEPYYSMYDPSQVVLDESFFKPLEGKPNHFRTISQMWGMWEASEDRWKEVIS
                     KFWGYITLIDDAIGELFRFLEENNLYENTFLVATADHGDAMGAHKMIEKGEFMFETTY
                     NIPLIIKDPHSTRVNEVDDNLVYLHDLTSTVYDVANQPIPTAFQGESILPITRQSQNN
                     DRKGLLCQLAGHFVYFEQRMWRRKDYKLVFNATDRCELYDVRKDPEEMHNLFYDEAYQ
                     AVKKEMLEEMRQEMKRLNDPLENWVYRIIDEI"
     gene            12296..13414
                     /locus_tag="PXE44_000905"
     CDS             12296..13414
                     /locus_tag="PXE44_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDL5588432.1"
                     /translation="MTTTYFHLMAKPSSFHCNIQCEYCFYLSKEQTIPPEKSQFMNDE
                     TLQNYIRHYIEANQSQRVDFVWQGGEPTLLGLDFYKQAVKYQQKFANGKQITNAIQTN
                     GIALNRQWASFFKENHFLVGLSIDGLSDIHDRYRISTNGKPTFERVKSAVDLLKEVGV
                     EFNTLTVVNDRNWQRGRETYQALKALGSRYMQFIPVVESHKGELQPFSVPPQGYGQFL
                     VDVFHEWLPDRGQIFVIEFDNLLGQWLGYPSNNCVFRPTCGSSLVVEACGDVYSCDHF
                     VYPDYHIGNLNQQQLAEIVSSAAQRQFGQNKQAKLSQACLNCKFRILCHGGCPKHRIV
                     PQANSENHNYLCPSYQQFFAETSSVMRQMSEQIRFAGK"
     gene            complement(13505..14086)
                     /locus_tag="PXE44_000906"
     CDS             complement(13505..14086)
                     /locus_tag="PXE44_000906"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014064645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL5588433.1"
                     /translation="MAKLYFYYSSMNAGKSTTLLQSSYNYQERGMNTLVYTAAIDDRF
                     GVGKVNSRIGISQEANLFTADTNLFAEIKALHSNKALHCILIDEAQFLTKTQVYQLSD
                     VVDKLKIPVLCYGLRTDFQAELFEGSKYLLAWADELEELKTICDCGKKAHFVVRLNEK
                     GEAIKDGEQIQIGGNDRYLSVCRYHYKQKLGKL"
     gene            complement(14142..14360)
                     /gene="zapB"
                     /locus_tag="PXE44_000907"
     CDS             complement(14142..14360)
                     /gene="zapB"
                     /locus_tag="PXE44_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HDL5588434.1"
                     /translation="MSLSVLDQLEEKIKQAVETIQLLQLEVEELKGKNDAAKQENDTL
                     RSEYEQLKVEQQNFQDRLRSLLGQIDNV"
     gene            14557..15575
                     /gene="glpX"
                     /locus_tag="PXE44_000908"
                     /pseudo
     CDS             14557..15575
                     /gene="glpX"
                     /locus_tag="PXE44_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484632.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
     gene            complement(15652..15975)
                     /locus_tag="PXE44_000909"
     CDS             complement(15652..15975)
                     /locus_tag="PXE44_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5588435.1"
                     /translation="MKLSSELQNQINQLNQLRKQALKQEQDEQKLFEKNKIEDLPHFG
                     TQLNAQRKLLGIELTTLEMQTGISSSTLKRLFKDPSQVKFQTICIVAETLGFNLCAIN
                     THKQE"
     gene            16191..17513
                     /gene="pepP"
                     /locus_tag="PXE44_000910"
     CDS             16191..17513
                     /gene="pepP"
                     /locus_tag="PXE44_000910"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDL5588436.1"
                     /translation="MDLAYLAKMPQEEFTARRERVLEQMQDNSALLVFTESEKRRNSD
                     SEYLFRPDSYFWYLTGLAEPKSAVLLVKSEGKTESIIFVRKKDPLMETWNGRRLGIEE
                     APKTLQLNAAFDIDDIETVLAKKLENLTACYCALGIQEWGDNILFATFEKMKVNRQKV
                     PTTLIDWQPMLSEMRLIKSEFEIALIQQACHISSMAHIRAMKQARPNRYEWEIEGEIQ
                     HEFSRFGARFPAYNSIVAGGENACILHYNENDQVLKGGDLLLIDAGAEFAYYAGDITR
                     TFPINGKFSEPQKELYELTLTMQKEAINLLVPNSSIKAANDKAVQILTQGLVRLGILN
                     GDVESLIEQKAYRQFYMHGLGHWLGLDVHDVGDYGTERDRPLQIGMVLTVEPGIYIPK
                     DADVPEQYKGIGIRIEDNLLITEYGNKNLSSGCPKEIADVEQLMQSLN"
     gene            17629..17814
                     /locus_tag="PXE44_000911"
     CDS             17629..17814
                     /locus_tag="PXE44_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HDL5588437.1"
                     /translation="MYFEIYKDAKEEFRWRLKAGNHEVIATNQAYTTKQGCQKGIEAV
                     KKVTPETEVKDLTKAEA"
     gene            17943..19259
                     /gene="argA"
                     /locus_tag="PXE44_000912"
     CDS             17943..19259
                     /gene="argA"
                     /locus_tag="PXE44_000912"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDL5588438.1"
                     /translation="MQNTELALAQWFRQSTPYVNMHRGKTFVIMLDGDVIESPNFVNI
                     INDISLLHSLNIKLVIVFGARPQINQLLKQNHIEPEYYHNIRVTNPQSLEYVKQAVGK
                     VHYDFFARLSLRLPHSEVINVVSGNMILAQPIGVVDGVDYTLSGKIRRINIESIKQQL
                     AQNSIVLLGPIAPSVTGEMFNLPFEDIATQVAIKLRADKLIGFCEEQGILDENGKPIP
                     DYLPQDGETLLQQLIERGEYHTPKARFLQAAITVCREGVKRSHLLSYKEDGSLLQELF
                     SRDGIGTQFSMESSEQVRIANSSDIPGLLDLIRPLEQQGILVKRSREQLEMEITNYTI
                     IERDGVVIACAALNPYPEEKMAEMACVAVHPDYRDSSRGDILLENIQKRAVQLGMEKL
                     FVLTTRTTQWFQERGFRLAGAQDLPRYKRENYNYQRRSKVLILPLW"
BASE COUNT         5824 a         3722 c         4323 g         5487 t
ORIGIN      
        1 gtttaaatat ttcaggaaaa gtatatggag tctttgattc actaccaatg ggttgaaaca
       61 aatgagcaat tagaggccgt atgtgaacag gccagaaaaa caaaagtagt tgccttagat
      121 acagagttta tccgtacacg tacctattat ccgatattgg gtttaattca actctttgat
      181 ggccaacagg ttagccttat tgatccaaat acgatttctg acttctcgcc ttttgtgtca
      241 ttattagccg ataaaaatat cataaaagta ttacatgcct gtagtgagga tttagaagtt
      301 tttgagtatc aatttaagca attgccagag ccgatgttgg atactcaggt gatggcagcc
      361 tttgccggca ttggtatttc gatggggttt gcaaaattgg tttcccacta cttaaatatt
      421 gaactagaca aaggcgcttc ccgaaccgat tggcttgcca gaccgctaag cgctgaacaa
      481 ttacaatatg cggcagctga tgtttggtat ttattgccag tttatgagaa attggcacaa
      541 gcattaacaa aaagcacttg gcaaagtgct gtagcagagg agtgtgtcgc tttgttgaag
      601 aaagcgaaac agcctatcaa taaggaaaaa gcctataaaa atattgccaa tgcttggcga
      661 ttgaatcggg aagagttagc catattacaa gtattagcga aatggcggat tgaagaagca
      721 caaaagcgca atcttgcctt aaattttgtc gtgaaggagg caagtttagt tcaaatcgca
      781 aaaagctgtc ctaaacacac ttcttcgctg ttagaattta tgcacccaaa tgaagtcaga
      841 attcacggaa aaaaaatact atggttggtt gagcaaggaa aagcaataga atcgaatgat
      901 tatcctgaac tcattcagcg cttggtggat gaaaagggtt ataaatatta tatgcaagcg
      961 atgttgcaaa aattattaga aattcaaccg cttgatatcg taccggagtt ggttgcaagt
     1021 aaacgtcaat taaatcagct atttaaatgg tatattgatg gaaaaccatc agaaaaaatt
     1081 cctgaattat taatcggttg gcgaaaacct tttggtgaac agctgctatc ggttttatct
     1141 taaatttcat tcgggttaca tagactatgt acagtataat tgcctaaaaa ggagtttata
     1201 tggaaaataa aaaaccacca aaagcattag atgcgattga tttaaaaatc ttaaacgagc
     1261 tacaacgtaa cgggaaaatt tctaatattg aattatcaaa gcgagttggg ctttctccaa
     1321 cgccctgctt agaacgggta aaacggctag aaaaaaataa cgtaattatg ggctataaag
     1381 ctttacttaa ccctgaatta cttgatgccc ctttactggt gattgttgaa attactttgg
     1441 tgcgtggtaa gcctgatgta tttgaagaat ttaataaagc gattcaacaa cttgatgaaa
     1501 ttcaagaatg ccacctggta tcgggggatt ttgattatct gttaaaaacc cgtgtggcgg
     1561 atatggtatc gtaccgtaag ttattaggca caacattgct tcgtttgccc ggcgtaaatg
     1621 acactcgaac ctatgtggtg atggaagaag taaaacaaac caactatttg ttgttaaagt
     1681 aaggagaatt gggtgattga acaacttaaa ccacacctaa aaggcaaaga gaacttgatc
     1741 aaacttatgc ttttgttgac cggctttttg ggcgtttatt tgctaattgc ttgggcaagt
     1801 tatagtccat tagataatgc ttggtctgtt gcaagcagca ttacacaaac aaccttgaat
     1861 aaaacaggtc actttggtgc ttggagtatt gatatgctct atgcaatgtt tggtaaggtt
     1921 gcagttttag tacctattgc attaattatt ttctcaattt atagcttggg aatgggttta
     1981 agtgtcgata tgaaatggaa aactgtcttc attcgcctag ccagtttctt attattgatg
     2041 gtcggtcttg ccgggttatt tagcgtgctt ttttcaaact cagcttatta tctgtcgggt
     2101 ggctttgtcg gtggagtttg ggagacaata ttatctgata ctatcggaca atttggtgcc
     2161 ttattgattg caatgctttt tactatagct ggcttatatt tttgctctgt tcaatcatta
     2221 ttgccaatat taacgcaatt ttatgagtgg ataatggcaa aatcagaaga gagcaaaaca
     2281 gctgaatctc ttcagcattt gcaaaatact gagcaaaaac aaccgcttgt aactgaaaat
     2341 gaagcggtgg aagttgtaga tgaccataaa ccgcaattta ccgatatctc cgcctttaaa
     2401 cgcccaaaca ttagtggttt aagacactcg gaaaatgtag agatagataa ccgagaaatg
     2461 tatgaaattg aaagagatct tgagctaccg actattaata ttttaataca gcctgatatg
     2521 gttaaccaag ttgcggaaca agctacatcg ggctatgagt taccaaacga agaatccaat
     2581 gtattgcaaa tgccgaccat tcgcttaaat accgctatcg aaaacgaggt ggatacaatc
     2641 gaaaacagtt catcattgga agacctgaaa atagcagaat ctatgttacc tcaaattcag
     2701 cttgataatg atattgaaga tactgaagaa gctattgacg atgtgcgtga aattacaccg
     2761 gaatttatgg taacgccgcc caaggcgatg aaagatgtgt ctgagcagcc agcctatcca
     2821 aaaggctatg gtgatacgtt aattcacccg ttgttacaac gaaaagtgat aaccgaaaaa
     2881 ccgacaacac cattgccaac gctagatttg ttggataaag caccgattca aagccagcaa
     2941 attaccgaac aggaaatacg agaaacctct gtgcggctag aagcagaatt agcaaacttt
     3001 ggcgtaaaag ctaccgttga agatgtattg gtcggtccgg tagtgacccg ttatgaaatc
     3061 caaccggctg ccggggtaaa agcctccaaa attaccaatt tagcgagcga tatagctcgt
     3121 ggcttaatgt ttaaagcgat taggattact gaagttatcc caaacaaacc ttatatgggt
     3181 attgaaactc cgaataaaca ccgtgaaaca gtgtggttgc gtgatgtgct agacagcgat
     3241 gaattccgca ataccactgc aacattgcca atggcactag gtaaagacat cagtggtgag
     3301 cctgtggtgg tcgatatggc gaaaatgcca cacttactgg tggcagggca gaccggtggg
     3361 ggtaaatcgg ttggggtgaa tacgatgatt ttaagtctat tgttcaaact tacaccagaa
     3421 caagtccgct ttattatgat tgaccctaaa gtggtggagc tttccattta taacgatatt
     3481 cctcacttat taacgccggt agtaaccgat atgaaaaaag ccgccaacgc actacgctgg
     3541 gcggtggagg aaatggaacg ccgttatttg ctggtaagcc atttacaggt gcgtaatatt
     3601 gaaggctata atgcgaaaat cgaccaagcg gcagcgatga atttgccgat tccagatcca
     3661 acgtggcgac cgggcgattc aatggatagc cttccgcctc cgctacaaaa attaagctat
     3721 attgtgctga ttgtcgatga gtttgcggat ttaatgatgt ctgccggtaa agaagtggaa
     3781 gagtacatta tgcgaattgc tcaaaaagcc cgagcggtgg gaattcactt aattcttgct
     3841 acccaacgtc cgtcaaccga tgtgattacc ggcgtgatta aagccaatat tccaagccgg
     3901 attgcgttta cagtagcgag ccaaattgac tcaagaacta ttttagattc aggtggagca
     3961 gaggcattat tagggcgagg cgatatgctc tattcaggag ctggcagccc ggatattatt
     4021 cggatacacg gtgcgtttat gaaagacgaa gaagtgcaac gcgtggcgga taattggcgg
     4081 gcaagaggaa aaccaaacta cttggaaagc attgtggaat cccgctcgga agaggctgat
     4141 ggcaaaaacg atggcggaac gggcgattta gatccgctat ttgacgaggt tgttgaatat
     4201 atcacggaaa ccggttcggt ctctatcagt aatattcaac gccgcttctc gcttggtttt
     4261 aaccgtgcgg ctagaattgt ggatcaaatg gaagctcaag gcattgtttc tgagccactt
     4321 aaaggtggca aacgagaagt gctggcgagg taaaacacat acaagcggtt agatttctgt
     4381 gaaattttgc aaattttgac aaaaatctaa ccgattgtat ttatctaatg ctaaataagc
     4441 atagtataag gctagtcttt gggctaaaat taacgcaatt tttacatctt ttaaggttga
     4501 atatatgcga ttagatgata ttaaagaaag taaaaatgtt gaagacagaa gattcaacaa
     4561 tgtgggtaga actaaggttg gtagagggaa aggtggaata ttaaccttta ttatcgtgct
     4621 tgttggagct tattatggtg ttgacttaac aggtttaatg ggcggtggcg tagaatatga
     4681 ggagcagaca tcagcattaa atgaaagtga agaaaaacag ctaagaacct tatccgctaa
     4741 agtattaacg accacagaaa atatttggac ttcctacttc cagcaaaatg gtctaactta
     4801 taaagagccg tctttggtgc tttaccgtgg ggcaattcaa actgcctgtg gcaccggtca
     4861 atctgcaatg ggtcctttct attgccctgt cgatcaaaag gtttatttag atttatcatt
     4921 ctatgatgat atgcgtaaaa aattaagagc atcgggcgat tttgccttct cttacgtgat
     4981 tgcacacgag gttggccacc acgtccaaaa tttattgggc ataacaagca aaacccaacg
     5041 ggctcaaatg aaagcctcat ctaaagccga agcaaaccaa atttcagtca atgtagaatt
     5101 gcaagctgac tgttttgccg gcgtttgggg acaccaactc gcaaaagcca accgattaga
     5161 ggaaggcgat gtggaagaag cctttaatgc tgcccaagca gttggagatg accgattaca
     5221 gcaacaaagt cgtggctatg tcgtgccgga tagcttcacc cacggctcat ctgcacagcg
     5281 tttggcttgg tttagaaaag gtttaaccac aggaaatcct gcagtttgta atacatttaa
     5341 ttaaaaaata attaaattat ttgaaatctt tttattatcg tactactatg atagtacgat
     5401 tttattttca ttttatttag agagtgaaca caatgtctta tatcaaaaat gacgatgtca
     5461 gaattaccag tattgaagaa ttactaccac ctattgcatt actggaagcc tatccggcaa
     5521 gtgatgttgc agcggaaacc gttgctaaaa cccgtcaatc aatccacaaa attttacacg
     5581 gtgcggatga tcgcttatta gtggtgattg gaccttgctc cattcacgac ccgaaagcgg
     5641 cattagaata tgcacaaaaa attaaagcaa tgcgtgctga tccgaaaatt aaccaaaact
     5701 tagaagtggt gatgcgtgtc tattttgaaa aaccccgtac aaccgttggt tggaaaggct
     5761 taattaacga cccttatttg aacgaaacct ttgccttaaa cgatggttta cgcattgccc
     5821 gtaaagtatt atctgatatt aatgatttaa gcgtgccgac tgccggcgag tttttggata
     5881 tgattacacc acaatatgtg gctgacttta tgagctgggg agcaattggg gcgagaacga
     5941 ctgaatctca agtccaccgt gaattagcct ctggtttatc ctgtgcggta ggttttaaaa
     6001 acggcacgaa tggtagtgtg aaaattgcat tagatgcaat ttcttccgca aaatcgccac
     6061 accatttctt atcggtcact aaattcgggc attctgcgat tgtttcaaca gcaggaaatc
     6121 ctgactgtca cattattttg cgtggtggcg atagcgcaac caactacgat gctgactccg
     6181 ttgcagcggc ttgtgctgcg attgagaaat ccggcggacg tccacacgtg atgatcgatt
     6241 tcagccacgc taacagccag aagcagttca aacgccaaat ggacgtttgt gctgatgtgt
     6301 gcaaccaaat tgcaggtgga tcagacttta tttcaggtgt gatgattgaa agccacttgg
     6361 tagaaggtcg ccaagactta attgaaggca aaggtttaga ggcgttagtt tacggacaaa
     6421 gtattacaga cggctgcatt ggctgggaag atagtgaaaa agcgttattc gcacttgctg
     6481 aagcggttga aaagcgcaga aattttgcaa aataatcttt tcttgaaccg agggcgaata
     6541 taattcgccc tttatttgtt taaccttaaa gcaaatttaa tttattcaaa ctaaacatat
     6601 gatgaaaccc agcattcttg ctttagatac tgcaacagag gcttgctctg tagcgttaca
     6661 gtataacgga aatatcacgt tcttagatga aatcagccct cgttcccata ctcaacgtat
     6721 tttgccgatg gtagatgaat tacttaatca agccaatatc gaactcaaag aagtaaatta
     6781 tttagtattc ggacgtgggc cgggcagttt taccggtgtg agggtcggtg taagtgtggc
     6841 acaagggttg gcaatgggag cgaatttgcc ggtggtagct gtttctaact taaaagcaat
     6901 ggcagaagaa gcataccaaa aattaggtgc aacaaaggta attgccttaa ttgatgcccg
     6961 aatgaatgaa gtttactttg ctcaatttga acgaaagggt gaagaatgga atgaagtggt
     7021 ggctgaacaa gtctgttcgc cagaagtcgc aataagtcaa ttccaaatga gtgaagagac
     7081 tgtggtggtt ggcacaggtt gggcggcata ttcgcaattt tctgagcaaa atctaccgct
     7141 tgttatcagt gatattacct tgccttccag ccgatatatg ctttcgattg ctgaaactga
     7201 gattcaaaaa ggcaatatcc aatctgcttt tgaaatagaa ccggtgtatt tgcgtaatga
     7261 agttacttgg gaaaaactac ctcataagca ataatctatt gaacttatgg ttaagtgaat
     7321 aaatattgca aaaagcccat ataacaggca atagtgaata ggattacacc aagtaattta
     7381 gtagttaatt cgggactcaa gatattgcgt aatttcattg aaaaatagac agtaaagaga
     7441 gaacttatca ctacggtgcc gacaattgca aaattcacat agccgatttg ccaagttgct
     7501 aaggttgaat tcaacacaaa agggcgggat aagtagccat ataaattgcc gattccgcca
     7561 atcaccatca tatagttagt ataaactgca acctgtttcg gtgaaaccga gcgtatttgc
     7621 gtgacaatcg gagccataat cgaaccgccg ccaatgccgg taatacctgc aattaaaccg
     7681 cctaaactgc acattgtggc attagatact gcttcttttg gtttaggtgg tactattgct
     7741 ttaactcttg gtttgtgaaa tagggtttta aatgaaagtg tcagcagggt tagaataaag
     7801 atagcgataa tgctgttatc agagaaaaga aaactgagtt caaacccaat ttgtacgcct
     7861 aacatcatcg caagtgacca aatcaacaca ttttttcgat tgatttttat ctgttgatgg
     7921 tggaataagc ataaattgat agtggctgaa ccaatcacaa tggtaagaga gcaggcggaa
     7981 atcacttgga gaggcagttc aggaaacagt gaatgtaaaa tcggcatcat taaaacaccc
     8041 ccgccaatcc caaatagtgc agaagtgata ttgactataa tgccacaaat gatcaaaata
     8101 attatcacgg tcattgtcat aatccatctc ctaaagtgtg ataagtatgc aaaattttct
     8161 aaataaagga ttatgatcgc aatcatatat ggaaaaaata aaataataag gataatcaat
     8221 gaacaatatg ccaacccccg aacaattgat gtcttgccaa ttaagtgtaa tgtctgatct
     8281 agaaaaagga gtcgtcattt attctcaggg ttatcacgcc agagaatttt attttttaaa
     8341 aagcggatta atagggctgt accatatgct tgaaaatggt aaagagagct tggtgagggt
     8401 atataaagcc aacgattatt ttgggtttag aacctttttt ggcgataatc gctaccattg
     8461 ctcggctcgg gtgttacagc cggcagaatt gatacgtatt acccccttaa atttcattga
     8521 gttttggtcg aataattccg ccttaattat ctctctgtta aaacagctct cgacagaatt
     8581 acaagacgca gagcatcgtt tatcaatggt ttcctatcaa aaaacgcaag agcgggtaat
     8641 gaacagtatt cattatttac accaacgtta tcccaattat cactggacta accgtgaaat
     8701 cgccgaattt gcaggttgtg aaaccgaaac agccattcga ttaaggcgag aattaaagaa
     8761 aatcagttaa attgtcataa attattcata gttgtgatca ccatcacata tctattatga
     8821 caaacatcat attctatcgt taaaaataag atttatgcta ttgccgtctc taatgggata
     8881 tatcatttat atagtcatat tgaggtgaag aatatgttat taacaataat tacctttctg
     8941 gttgtaactg gattagtcgg gtatatctct tggctaaaaa ccagaaacga cgatctttcg
     9001 acatctaaag gttatttctt ggcagggcgt ggcttgagtg ctacggttat cggctgttcg
     9061 atggtcttaa cctccctttc taccgagcaa cttatcggcg tgaatgccaa ctcttatgcc
     9121 ggtaatttct caattattgc gtggactgtg ccggcggtaa ttccgctttg cttcttggca
     9181 ctttatctgt tgccgaaata tatccgcaac ggttatacca ccattccgga gttctttgaa
     9241 aaccgtttcg accgccaaac ccgtttgatt atgtcgggct tgtttttggt gttctatctt
     9301 ttcatcgtca ttccgactgc actttacacc ggtgcgattg ccttcaataa aatcttcaac
     9361 cttgaaactg ccttcggctt aagctatggg gcagcgatta cctatacggt gattgcgatt
     9421 ggtattgttg gggcgattta tgccattttc ggtggcttaa aagcggtggc ggtgtcagac
     9481 acttggaatg cggtcttgtt agtcattggg gcattattag tgccggtgtt tgcacttatc
     9541 tatttaggta atggcagtat ggcagaaggg gtgaaaatca tcaccactac tcacgttgaa
     9601 aaatggaatg cggtgggtag tgccaccgat gcagtgcctt ggccgaccat tttcaccggt
     9661 attatggtgg tgcatttctt ctactggacg accaaccaag ccattgtgca acgtgccttg
     9721 ggggcgagag acttaaaagc cggtcaaaaa gggatcttaa ttgcggcact gttcttatta
     9781 ttgctaccgc ttatcttaaa cttaccgggc ttactcagtt tccatatttt aggtgagggc
     9841 ttgaaaccga ttgatgtgtc ttaccctgaa ttagtgaata aaactttccc aaccgtgcta
     9901 caaggcttct ttattgccgc actgttcggg gcgattttga gtaccttcaa ctcgttctta
     9961 aactcagcgg caaccattta ctgtaaagac ttactgccgt ctatcagcaa aaaacagttc
    10021 acagatgcgg aactgataag ttatgcgaaa aaagtctctg cggtaatggc ggtgattacg
    10081 atgatctttg ggccgttact gatgttcggc acagacggta ttttcttgct gaccaaacgt
    10141 ttcgccggtt ttgtgaacat tccgattgtg gcactgtttg tcatcggctt attcaacaaa
    10201 accgtctcag gcaaagccgc acgcattgcc ttactcgcac acgttattct ctacttctgc
    10261 atcgtttggg tgtttgatgt aaaaatcaac ttcgtgtatg tgcaagctgg cttgttcgtg
    10321 ttcgatgtgg cgttaatgct gattttagga attttcctaa aacgtgcgac tccatacgaa
    10381 gacaacaaaa tcaaccacgc caatgtggac ttaaccagct gggaatatgc cccactggca
    10441 gtggcttcta tcgtgttggg cttattcacg ctatacgctt tcctctctcc gctcggttta
    10501 gcctcaccag acggcaatcc ggcaatggtg ctggcggtgt atgctgtgct acaagcggtg
    10561 gtttgtgtcg tgttttttgc aaaatgtaaa ggcaaaaagg cggtgaatta aatgaatgtg
    10621 agccttctcc tcattagtat aaaaatacca agaacggaaa aggctcgaga tcaaaaatcc
    10681 cctctttgct aaagagggga acagatctcc gaaacttgat aatttatcgg ttattaacaa
    10741 gccttaaaga ggtcattatg aataacatca tctatatttt gttagatcaa gtgcgtaaag
    10801 atatgctcgg cacttacggg catcaggtgg taaaaacccc gaatttggat cgcttggcga
    10861 aagacggcat tcggtttaac aacgccttta cgccggcttc ggtctgcggg ccggctcgca
    10921 cctcgctgtt taccggctta atgccgtcca gccacgggat tatcaaaaac ggcgaaaaag
    10981 gcggcacagg cgaaattcca caacaaaacc ctaatattgc cggcgtggag ggctataact
    11041 cctttgtgat cggcaaatgg cacgttggta caaaatccgt tcccgaagat tatggcatcg
    11101 ccggtcataa ctttgacggc tatggctacc cgggcagcca agtgtataaa aacctcgtgt
    11161 ttaatcagcc gccaacacac tccaaccgtt atcgtgaatg gctggcggaa aagggctatg
    11221 acgcacccga agtcagtcga gcctattttg gcgacaatcc acacctgcgt gtgcaggaac
    11281 tctgtggttt gctttccggc agtcgcgagc agacaataga gtggtttatc attgacgagg
    11341 cgaaaaaata cattcaagcc tcccttgatg agaaaaaacc gttctttgcg tggatcaact
    11401 tctgggggcc gcacactccg tgtgtagtgc cggagccgta ttattcaatg tatgacccaa
    11461 gccaagtggt attggacgag agtttcttca aaccgcttga gggcaaaccg aaccacttcc
    11521 gcaccatttc acaaatgtgg ggaatgtggg aggcgagcga agatcgttgg aaagaggtga
    11581 tttctaaatt ctggggctac atcacgctga ttgacgatgc cattggcgag ctgttccgtt
    11641 tcttggaaga gaacaatctc tatgaaaata ccttcctcgt tgctaccgcc gaccacggcg
    11701 atgcgatggg ggcgcataag atgatcgaaa aaggcgagtt tatgtttgaa accacctaca
    11761 acattccgct gattattaaa gatccgcact ctacaagggt gaacgaagtc gatgacaacc
    11821 tcgtttattt gcacgatctc acttccaccg tgtatgatgt ggcaaatcaa ccgatcccga
    11881 ccgctttcca aggcgagagt attctgccga taaccagaca aagccaaaac aacgaccgca
    11941 aaggcttgtt gtgccaactg gcagggcatt ttgtctattt tgaacagcga atgtggcggc
    12001 gtaaggatta caaattggtc ttcaacgcca ccgaccgctg cgaactctac gatgtcagaa
    12061 aagaccccga agagatgcac aacctctttt atgacgaagc ctaccaagcc gtgaaaaaag
    12121 agatgttgga agaaatgcga caggaaatga aacgcctcaa cgacccactg gaaaattggg
    12181 tgtatcggat tattgatgag atataattgt ttgtagaggc gggttttata cccgcccgaa
    12241 aattaatgta aacaatgggc gaacataaag tccgccccta caatttagag atacaatgac
    12301 caccacctac ttccacctaa tggcaaaacc cagcagtttt cactgtaata ttcagtgcga
    12361 gtactgtttt tatctgagca aggaacagac cattccgcca gaaaaatcgc agtttatgaa
    12421 cgatgaaacg ctgcaaaact acattcgtca ttacattgaa gccaaccaaa gccaacgggt
    12481 ggattttgtg tggcaggggg gcgagccgac cctgctgggc ttagatttct acaaacaagc
    12541 ggtcaaatat caacaaaaat ttgcaaacgg caagcagatc accaacgcaa tccaaaccaa
    12601 cggcattgcc ctaaaccgcc agtgggcgag ctttttcaaa gagaatcatt ttctggtggg
    12661 cttatccatt gacggactga gcgacattca cgaccgctac cgcatttcca ccaacggtaa
    12721 accgaccttt gaacgggtga aaagtgcggt cgatttattg aaagaggttg gcgtggaatt
    12781 taacaccctc acggtggtga acgaccgcaa ctggcaacgg ggcagagaaa cctatcaagc
    12841 cctcaaagcc ctcggtagcc gttatatgca gttcattccg gtggtggaaa gccacaaggg
    12901 ggaattacaa cccttttccg ttccgcccca aggctacggg caatttttag tggatgtttt
    12961 tcacgaatgg ctgcctgacc gtgggcagat ttttgtgatc gagtttgata acctgctcgg
    13021 gcaatggctt ggctatccct ccaacaactg tgtattccgc ccgacctgtg gcagctcgct
    13081 ggtggtggaa gcctgtggcg atgtgtacag ttgcgaccat ttcgtctatc ccgattacca
    13141 catcggcaac ctcaaccaac agcagctggc agagatcgtc tcatcagccg cccaacgcca
    13201 attcgggcag aataaacagg caaaactctc gcaagcctgc ctaaactgca aattccgcat
    13261 actttgccac ggcggctgcc ccaaacaccg catcgtcccc caagcaaaca gcgaaaacca
    13321 caactacctt tgcccctctt accaacagtt ttttgccgag acttcgagtg tgatgagaca
    13381 aatgagtgag caaattcggt ttgcaggaaa gtaaagagag gtaaataaca caaacaagcg
    13441 gttaaatttc gctgaaaatt tgcaaaaatt cccgaaaatt taaccgcttg ctgcttcttg
    13501 tagcttatag cttacctaac ttttgtttgt aatgataacg gcaaacagag agatatctat
    13561 cattgccgcc gatttgtatt tgttcaccgt ctttaatggc ttcgcctttt tcatttaatc
    13621 gaacgacaaa atgggctttt ttgccacaat cacagatggt tttgagttct tccagctcat
    13681 ctgcccaagc caacaaatat ttgctacctt caaataattc agcttggaaa tcggtgcgta
    13741 gcccgtagca aagcaccgga attttgagtt tatcgaccac atcacttaat tgataaacct
    13801 gtgttttagt taaaaactga gcctcatcaa tcaagataca gtgtaacgct ttgtttgagt
    13861 gtaatgcctt aatttccgca aatagattgg tatcagcggt aaataaattg gcttcttgtg
    13921 aaataccgat acgagagttg actttcccca caccgaaacg gtcatcaata gcggcggtat
    13981 aaactaaagt gttcattcca cgttcttggt aattataaga agattgaagc agagtagtag
    14041 atttgcctgc attcattgag gagtagtaaa aatagagttt tgccatagct gtaacaaata
    14101 aaaaacggct aaaaatttag ccgtttgtgc tgaaaataga attaaacgtt gtcgatttga
    14161 cctaaaagtg aacgtaaacg atcttggaag ttttgttgtt ccacttttaa ttgttcatat
    14221 tcgctgcgca aggtgtcgtt ttcttgtttc gctgcatcgt ttttaccttt taattcttca
    14281 acttctaatt gaagtaattg aatagtttca acagcttgtt taattttttc ttctaattgg
    14341 tctaaaaccg ataatgacat agtaaattcc ctctaaaata atataaaaac ttgtaaagat
    14401 tatactctaa atctgacttt atttttataa aaaagtttgg ctatttgtga atttttattc
    14461 aaacgctgat tttttagtaa cgtagcttgc aggttactga taaaatataa tctctcttct
    14521 tttaaagttt ttctttcaag aaactaagga acaaaaatga aacgaactct ttcttttgaa
    14581 ttttctcgtg ttacggaagc agcggctcta gcagcttact cttggctagg ccgtggggat
    14641 aaaaatgccg ctgatgaagc tgcggtgaaa gcaatgcggt ttatgctgaa ccaaatggaa
    14701 atccgtggcg aagtggtaat tggtgagggc gaaattgacg aagcaccgat gctttatatt
    14761 ggcgagaaaa tcggtctatc ccgcctagaa gatgaggaaa tcagtattgc ggttgacccg
    14821 attgacggta ctcgtatgac tgcaatgggg caggcaaatg cgttgccggt attagcagca
    14881 ggcggcaaag aaaccttttt aaaagcccct gatatgtata tggaaaaatt agtggtgggg
    14941 caggaatgca aaggaatgat tgatttaaat cttccgcttg aacaaaatct tcgccgtgtc
    15001 gcctcgaaaa agggcaaatt actttcccaa ttaaccattg ctatcttgct aagcctcgcc
    15061 acgaaaaaat aattgccgat gtgcaaaaat taggggtaag agtgattgcg attcctgatg
    15121 gcgatgttgc ggctgcggtg caatgctgtt tgccagagag cgaattggat ctgctttatg
    15181 gtatcggcgg agcacctgaa ggggtagtgg cgggggctgc ggttcgtgca ttgggtggcg
    15241 atatgcaagc acgtttaatt cctcgcaacc aagtgaaggg gaatagcccg gaaaatttaa
    15301 ctgctaccga aaaagagctt ttacgctgta atgaaatgaa tgtaccggtg aatacggtgt
    15361 tgaaattaga agatttggtg cgtgatgaca atatcgtctt tgttgcaacc ggcattactt
    15421 caggtgaatt gttaaaaggg attaaacgta gaggcaatat tgccagcact gaaaccttac
    15481 ttattcgtgg caaatctaga accattcgta aaattcaatc cgaccattat gtcgatcgta
    15541 aagataacga actcctttct ttattggatt tatagctttt ctctgataaa tcgggctttt
    15601 agcccgattt tttgtcttcc caaagtaatc aaaatcacta cattgtcacg cttactcttg
    15661 tttgtgagta ttaattgcac ataaattaaa gccgagtgtt tctgccacaa tacaaattgt
    15721 ctgaaatttc acttggcttg ggtctttaaa taagcgtttt agggtagagc ttgaaatgcc
    15781 ggtttgcatt tcaagggtgg ttaattctat gcctaatagt ttcctctgag cattaagctg
    15841 cgtgccaaaa tgtggcaaat cttcaatttt atttttttca aacaatttct gctcgtcttg
    15901 ctcttgtttg agtgcctgtt ttcgtaactg atttagttgg tttatctgat tttgtagttc
    15961 gcttgatagt ttcattttat ttttgagcta aaagtagatt atttatgtgt tcataggtta
    16021 aatataacct gtttagatat gtaaaggtta tatttaatct taatctgttt tgtttttgca
    16081 atcaagatct caaatataat ctttcatctg ttttggttag gctgaattta tgctgcgttt
    16141 gtgctaaaat aaggcaggtt tcgatataga tataataaaa aggagaaata atggatctcg
    16201 cttatttggc taaaatgcca caagaagaat ttaccgcacg ccgtgagcgt gtgcttgagc
    16261 aaatgcagga taattctgcg ttattggttt ttaccgaatc ggaaaaacgc cgtaatagtg
    16321 actctgaata cttgtttaga cctgatagtt acttttggta tttaaccggt ttggcagaac
    16381 ctaaatcagc cgtattgctg gtaaaaagcg aaggtaaaac ggaaagtatt atttttgtgc
    16441 gtaaaaaaga ccctttaatg gaaacctgga atggcagacg tttagggatt gaggaagctc
    16501 caaaaacgtt acagcttaac gcagcttttg atattgatga cattgaaacc gttcttgcaa
    16561 aaaaattaga aaatctgacc gcttgttatt gtgcgttagg gatacaagag tggggcgata
    16621 atattttgtt tgcaacattt gaaaaaatga aagtcaatcg gcaaaaagtg ccgacaacct
    16681 tgattgattg gcagccaatg ttatcggaaa tgcggttgat taaatcagaa tttgaaattg
    16741 ccttaattca gcaggcgtgt catatttctt caatggcaca tattcgagcg atgaaacaag
    16801 ctcgcccgaa tcgttatgag tgggaaattg agggggaaat tcaacacgaa tttagccgtt
    16861 ttggagcaag gtttccggca tataattcaa tcgttgccgg cggtgagaat gcctgtattc
    16921 ttcactacaa tgaaaatgat caggtgctta aaggcggcga tttattactg attgatgcag
    16981 gggcggaatt tgcgtattac gccggcgata ttacccgaac cttccctatt aatgggaaat
    17041 tctccgaacc gcaaaaagag ctgtacgaat taaccttaac tatgcaaaaa gaggcgatta
    17101 atcttcttgt gccaaacagc tcgattaaag cggcaaacga taaggctgta cagattctca
    17161 cgcagggctt ggttcgctta ggaattttaa acggtgatgt ggaaagcctg attgagcaaa
    17221 aagcctatcg ccaattttat atgcacggat tagggcattg gctagggcta gatgttcacg
    17281 atgtggggga ttacggcacg gagcgggatc gtcctttgca gattggtatg gtgttaactg
    17341 ttgaacccgg tatctatatt cctaaagatg cggatgtacc ggagcaatat aaaggtattg
    17401 ggattcggat tgaagataat ttgctgatta ccgaatacgg caacaaaaac ttaagcagcg
    17461 gttgccctaa agaaattgcg gatgttgagc agttgatgca atcgttaaat taattcgcaa
    17521 atttttgtaa aaaaatgacc gcttgctttg atattttggg gaatttagct taagctaacc
    17581 cgacacaaac acagtgtttg ggcaatcaca acacgaagga gtgtttatat gtattttgaa
    17641 atttacaaag atgcaaaaga agaatttcgt tggcgtttaa aagcgggtaa ccacgaagtt
    17701 attgcaacca accaagctta tacgactaag caaggttgtc aaaaaggcat tgaagcagtt
    17761 aaaaaagtta cacctgaaac agaagtaaaa gatttaacca aagctgaagc ataatctata
    17821 aagccctgat attatttcag ggctttgtta tttatgcaaa tataatgctt aattattcaa
    17881 tttaaggctt gcggattttt ctaaaaaaga taatatgcgt ttatattata aagaggaaat
    17941 atatgcaaaa cacagaactc gcattagctc aatggtttag gcaatctaca ccttatgtta
    18001 atatgcacag aggcaaaacc tttgtgatta tgcttgatgg cgatgttatc gaaagcccga
    18061 attttgtcaa tattatcaat gacatcagtc tcctgcacag cctgaatatc aaattagtga
    18121 ttgtatttgg ggcaagaccg caaattaacc aattgttaaa acaaaatcat attgagccgg
    18181 agtattacca caatatccgt gtgactaatc cacaatcgct agaatatgtg aaacaagcgg
    18241 tagggaaagt tcattatgat ttctttgccc gcctttcttt gcgtttaccc cattctgaag
    18301 tgattaatgt agtcagtggt aatatgattt tagctcagcc gattggcgtg gtggatgggg
    18361 tagattatac actaagcggt aaaatccgtc gtattaatat tgagagcatt aagcagcaac
    18421 tggcacaaaa ttcgattgta ttacttggtc cgattgcccc gtctgttacc ggtgaaatgt
    18481 ttaatctgcc gtttgaagat attgccacac aggttgcaat taaacttcgt gccgataaat
    18541 taatcggttt ttgtgaagag caggggattt tagatgaaaa cggcaagcca attccagatt
    18601 atcttccgca agatggcgaa accttgttac agcagttaat cgagcgtggc gaatatcaca
    18661 ccccgaaagc tcggtttttg caggcggcga tcacggtatg ccgagaggga gtgaaacgct
    18721 cgcatttatt aagctataaa gaagatggct cattgcttca agagttattc tctcgtgatg
    18781 gtattggcac acagttctca atggaaagct cggaacaggt acgtattgct aattccagtg
    18841 atattccggg attattggat ttaatccgcc cacttgaaca gcagggcatt ttagtcaaac
    18901 gctctcgaga acaattagaa atggaaatca caaactacac cattattgag cgtgatggtg
    18961 tggtgattgc ttgtgcggca cttaacccgt atccggaaga aaaaatggcg gaaatggcgt
    19021 gtgtggcggt tcaccctgat tatcgtgatt cctcaagagg ggatattcta ctggaaaata
    19081 ttcaaaaacg ggcagtgcaa ctgggaatgg agaaactctt tgtgctgact acaagaacca
    19141 cacaatggtt ccaagagcga ggatttaggc tggcaggtgc ccaagattta cctcgctata
    19201 aacgggaaaa ttacaattat caacgtcgtt ctaaagtatt aattttacca ctttggtaaa
    19261 atattcaatg tttttgaccg cttgttacca agagaactat aaaaggagaa ctatgaaaaa
    19321 acatcaaccc atcaaagggg ctatggcaat tattac
//
LOCUS       DANLZC010000033        19249 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.033, whole genome shotgun sequence.
ACCESSION   DANLZC010000033 DANLZC010000000
VERSION     DANLZC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19249
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.033"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            121..369
                     /gene="rpsP"
                     /locus_tag="PXE44_000913"
     CDS             121..369
                     /gene="rpsP"
                     /locus_tag="PXE44_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL5588439.1"
                     /translation="MVTIRLTRGGAKKRPFYQIVVVDSRSPRDGRFIERIGFFNPLAA
                     GQAERLRLDVAKVDAWVAKGADLSDRVASLVKEARKAA"
     gene            402..929
                     /gene="rimM"
                     /locus_tag="PXE44_000914"
     CDS             402..929
                     /gene="rimM"
                     /locus_tag="PXE44_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL5588440.1"
                     /translation="MSEQKIEVVGKLGSTYGIRGWLRLYSSTEQTESIFDYQPWFLKI
                     KGQWQQVELESWRYHSNDLIVKLKGTEDREAAQLLTNAEIGVDLSVFPELEEGDYYWH
                     DLIGCSVVNLEGYTMGTVAEMMETGSNDVLVVRNNGKDAFGKQERLIPFLYEQVVKRV
                     DLATKTITVEWDAGF"
     gene            974..1729
                     /gene="trmD"
                     /locus_tag="PXE44_000915"
     CDS             974..1729
                     /gene="trmD"
                     /locus_tag="PXE44_000915"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL5588441.1"
                     /translation="MWIGIISLFPEMFKAITDFGVTGRAVKQNLLQVECWNPRDFTFD
                     KHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKKAALAVEGGEAKVIYLSPQGRKLDQNG
                     VQTLASNQKLILICGRYEGVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFV
                     PGVLGKQASALEDSFAEGLLDCPHYTRPEVLDGMPVPEVLMSGHHEQIRKWRLEQSLE
                     RTWLRRPELLDSLALTDEQRVLLAKIKKQHKIS"
     gene            1756..2106
                     /gene="rplS"
                     /locus_tag="PXE44_000916"
     CDS             1756..2106
                     /gene="rplS"
                     /locus_tag="PXE44_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL5588442.1"
                     /translation="MSNIIKQLEQEQLKQDLPSFRPGDTLEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTLRKVSNGVGVERVFQTHSPVIDSISVKRKGAVRKAKLYYLRE
                     RSGKSARIKERLGD"
     gene            2262..2618
                     /locus_tag="PXE44_000917"
     CDS             2262..2618
                     /locus_tag="PXE44_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588443.1"
                     /translation="MKQLSIRLPDDEFTFIKTFASAHKLTLQNFVMSAIQAKVAEMSA
                     KNKISEESNIHQPLSKDEKIMLNEAKVFYSARSHLKAENVILPSDFMEQIKSGCGAEK
                     MAEMMGYTKEDWESMK"
     gene            2620..2943
                     /locus_tag="PXE44_000918"
     CDS             2620..2943
                     /locus_tag="PXE44_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588444.1"
                     /translation="MGQITFSNIFTKVIFENVLSASTKEKIKQMLFQSVVALPPLHSE
                     RKMILQLKKQKITIFYQVIEEQSVQILAYQFGGTDKLTGVSKAHFKTLDAIFQMTDEE
                     KEGKF"
     gene            complement(2953..3285)
                     /locus_tag="PXE44_000919"
     CDS             complement(2953..3285)
                     /locus_tag="PXE44_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5588445.1"
                     /translation="MQIEQYQQWVRQFYQQQGWYERSPFMRVTYLAEEIGEVACAVRA
                     IEIGRERPDETEASQQQKRDNLIEELGDVMDNLFVLADKYGISMTEVLERHQAKFEKR
                     YLQKNMEK"
     gene            complement(3378..4574)
                     /locus_tag="PXE44_000920"
     CDS             complement(3378..4574)
                     /locus_tag="PXE44_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL5588446.1"
                     /translation="MNIPFCLPENISPETFLREYWQKRPLLIRNGLPQIVGLFEPEDI
                     LELALEEEVTARLIQCENEKWSVKSSPLVEEDLEELPVQWSVMVQNLEQWSPELGELW
                     QAFGFIPQWQRDDIMVSCSPLNGTVGKHYDEYDVFLVQGYGQRRWQLGKWCDPSTEFK
                     PNQPIRIFDDMGELVMDEVMNPGDVLYVPSRMAHYGVSESEQGLTFSFGLRYPNATDL
                     LENLCKTIEQQADNINSSEFHLPFRLTPNEQPNALLDPKMVKMFKHQLIDLLQNSEQF
                     DELLTHSVATAASSRRYELLQTDNEYYPDEVQEILEAGGWLQQDANVKILYTENPQRV
                     YVNGEWIDELNEAEMALLIRIANGDSISWNKLASKVQDQAELELMLDTICDWLDSGWI
                     ILSEAE"
     gene            complement(4723..6066)
                     /gene="accC"
                     /locus_tag="PXE44_000921"
     CDS             complement(4723..6066)
                     /gene="accC"
                     /locus_tag="PXE44_000921"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL5588447.1"
                     /translation="MLEKVVIANRGEIALRILRACKELGIKTVAVHSTADRELKHVKL
                     ADETICIGPAPSVKSYLNIPMIIAAAEVTNADAIHPGYGFLSENANFAEQVEKSGFIF
                     IGPTADVIRLMGDKVSAINAMKKAGVPCVPGSDGPVGNDPKKNKEIAKRIGYPVIIKA
                     SGGGGGRGMRVVHHEKDLEESIAMTKAEAKAAFNNDMVYMEKYLENPRHIEIQVLADT
                     HGNAVYLAERDCSMQRRHQKVVEEAPAQGITPELRQFIGERCANACREIGYRGAGTFE
                     FLYENGEFYFIEMNTRLQVEHPVTEMITGVDLVKEQLRVAAGLPLSITQDDIKVKGHA
                     IECRINAEDPNTFLPSPGKITRLHVPGGLGVRWDSHIYSGYSVPPHYDSMIGKLITFA
                     ENRDIAIRRMENALSETIVEGIKTNIPLHTQILADKEFRKGGTNIHYLEKKLGLK"
     gene            complement(6138..6605)
                     /locus_tag="PXE44_000922"
     CDS             complement(6138..6605)
                     /locus_tag="PXE44_000922"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL5588448.1"
                     /translation="MDIRKIKKLIELVEESGITELEVSEEEGTVRISRAAPAVAPAAV
                     QYAAAPVVAPAAPQVASTVPVAAPAEAPKAAETSGHAVLSPMVGTFYRSPSPDAKPFV
                     EVGQTVKIGDTLCIVEAMKMMNRIESDKAGVVKEILVNDGEPVEFDQKLFIIE"
     gene            complement(6858..7640)
                     /locus_tag="PXE44_000923"
     CDS             complement(6858..7640)
                     /locus_tag="PXE44_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL5588449.1"
                     /translation="MAVKNEQFIVICNGVFRLFGNIVKILSYPFHALFPKKRFTIPEY
                     SPAKIQSSIPSKITKTIWQTNYSNKVTLPMYANYLFNRLMSLSYDYRYVSTEEREAYI
                     KANADERTFNAYSKLTDGAAQADFWRIFTLLREGGIYIDIDGHLVYPLSKIIGENDSE
                     VLIKRRDKYTNFFLASEAGNWILKDTLELIISNIENRRIEGGVFVMTGPDTLNNAIGD
                     KEVNFRRDKVTCAQGTFTNEYFQYIDKPGSKWNYKKNEDLLK"
     gene            complement(7650..8087)
                     /gene="aroQ"
                     /locus_tag="PXE44_000924"
     CDS             complement(7650..8087)
                     /gene="aroQ"
                     /locus_tag="PXE44_000924"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HDL5588450.1"
                     /translation="MKKILLLNGPNLNMLGKREPQIYGSQTLADIENHLKKLAAAQDV
                     ELESFQANGEEPIINRIHQAFQHTDFIIINPGAFTHTSVAIRDALLSVSIPFVEVHLS
                     NVHSREPFRHHSYLSDIAKGVICGLGAKGYEYALDFAISHLKA"
     gene            complement(8173..8541)
                     /locus_tag="PXE44_000925"
     CDS             complement(8173..8541)
                     /locus_tag="PXE44_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5588451.1"
                     /translation="MAFTIRKMVEQDTHQVFPLMQKLAVFEHYIDSFAITPEVVMESG
                     FRKSPPDFYCLVAENASNIIGILVYYFLPYTAQNRPAIYMKELYVDETARNQKVGEAL
                     MQALKNEAKAHHCKTIKSLE"
     gene            complement(8601..9425)
                     /gene="dapD"
                     /locus_tag="PXE44_000926"
     CDS             complement(8601..9425)
                     /gene="dapD"
                     /locus_tag="PXE44_000926"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDL5588452.1"
                     /translation="MSLQAIIEAAFERRAEITPKTVAAETKAAVEEVIAGLDNGSLRV
                     AEKKDGEWVVNQWVKKAVLLSFRIADNEIIDGAETKFYDKVPTKYGNYTEEQFKADGI
                     RAVPGAVVRKGSHIEKNVVLMPSFVNIGAYVGEGTMVDTWVTVGSCAQIGKNVHLSGG
                     VGIGGVLEPLQANPTIIGDNCFIGARSEIVEGVIVEDNCVISMGVFIGQSTKIYDRET
                     GEVHYGRVPAGSVVVSGSLPSKDGSHNLYCAVIVKKVDEKTLSKVGLNDLLRSIDE"
     gene            9573..10235
                     /locus_tag="PXE44_000927"
     CDS             9573..10235
                     /locus_tag="PXE44_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL5588453.1"
                     /translation="MENFANLLTGKTREHLVPLPNALSDKHYLQPQVVEAFLTLQHAA
                     KQAGFNLQPASTFRDFERQKGIWNAKFRGERKVHDDNGVAIDMAALSDLEKCKAMLRW
                     SAVPGASRHHWGTEIDIFDPDLLPENTPLLLEPWEYLEGGYFADLTKWLHVNAETFGF
                     YFPFDGKHSRVGFEPWHISYRPISNEYEQRFNDEILKAAWQDEEVAGKECLLEHFEEL
                     LK"
     gene            10232..10984
                     /locus_tag="PXE44_000928"
     CDS             10232..10984
                     /locus_tag="PXE44_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5588454.1"
                     /translation="MIQLINETQQHDKFRQICDKWQLVEDKSSPLALVLTEERLELRK
                     LDEPKLGAIAVNFVDGTMAHRRKFGGGRGEAVAKAVGIKGDYLPTVIDATAGLGRDAF
                     VLAAVGCKVLLVERNPIVAALLEDGLNRAYQDVEIGSFMQERMILADVRDISQLDPQS
                     QMAEVVYLDPMYPHKQKSALVKKEMRVFQHLVGADLDSDSFFLPAKGLAKKRVVVKRP
                     DYAPFLAEQKPDFSHTTKNHRFDVYLSHLKND"
     gene            11075..11911
                     /gene="ftsN"
                     /locus_tag="PXE44_000929"
     CDS             11075..11911
                     /gene="ftsN"
                     /locus_tag="PXE44_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDL5588455.1"
                     /translation="MPQRDYVARSNEKKSKKNQKESNVKKILMRIIGVILFFGIGLWF
                     LKSSAPVPTVPSAPQPANSQTKSELPSRPEETWSYIRDLETRTVITDNSQTSQERLAQ
                     LTAEQKQQIEERRKQEEARLAAEQAQQKPATDVSATELAPKTEEVKSLTEEELAAKKA
                     QEQALAQKQAEEKRRQAELKKQQEAKKEQPAKIVASAATLADKAANQAGKFGLQCGAF
                     KNKAQAENMQARLAMAGFNARIASSADWNRVFVGPIGDRAATSRAQSNARSVAECVIV
                     AM"
     gene            complement(11953..13134)
                     /locus_tag="PXE44_000930"
     CDS             complement(11953..13134)
                     /locus_tag="PXE44_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL5588456.1"
                     /translation="MKTELDMIVIGGGMIGAATALGLAKQGLRLALLEKNPLPTFNPD
                     SDYDLRISAISMGSIQLLEKLGVWEQIVAMRSTPYTQLETWEIDGFNTLFSAADLGLE
                     KLGDMVENNVIQIALWEQLKQYPNCLQAVGFEQIFANKAGEYWEVTVDGNTFAAPLLI
                     AADGANSQVRQWAGIGLTAWQYRQHCLLATVTTEKPAPSGTWQQFYPTGPRAFLPLSG
                     NHGCVVWYDSPQKIAELKMLPAEKLGVAIEQHFPSRLNAELGNIQVQASGAFPLTRQH
                     AQHYVQNGVVLVGDAAHTINPLAGQGVNLGFKDVKTLLEVIEQAVKKGKNFASEQVLQ
                     RYEQKRKPDNLLMQTGMDVFYKAFKTDLLPLKIARNVGLVLADKVSPIKKQALKYAIG
                     I"
     gene            13241..13579
                     /locus_tag="PXE44_000931"
     CDS             13241..13579
                     /locus_tag="PXE44_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588457.1"
                     /translation="MSCKTHANHEHEHGEGCGHTAIKHNDHIDYLHDGHLHHNKHGDH
                     YDEHVLEVNETNPADCNAAHCCGDHVHGEVCGHEAVPHGDHIDYIVNGRLHHQHGDHC
                     DDHGPVEIVK"
     gene            13711..14580
                     /locus_tag="PXE44_000932"
     CDS             13711..14580
                     /locus_tag="PXE44_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDL5588458.1"
                     /translation="MTLAIVYSRASIGVEAPLVTIEVHISNGQPKLTIVGLPEATVKE
                     AGDRVRSALLNANFIYPPQRITINLAPADLPKEGGRFDLPIAIGILAASGQIDSDLLT
                     QFEFLGELALTGHLRGVHGTIPAVISADKAKRQMILAKQNANEASLVSNATTYFAGSL
                     LEVVNMLNKRDKLPICQHISQHSAEIRPLVSRDLTDIIGQQHAKRALMIAAAGQHNLL
                     FLGPPGTGKTMLASRLADLLPEMTDEEAIETASVTSLVQNELNFQNWKQRPFRSPHHS
                     ASMVALVGGGCEN"
     gene            14672..16483
                     /locus_tag="PXE44_000933"
     CDS             14672..16483
                     /locus_tag="PXE44_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588459.1"
                     /translation="MFYLNNDNFKREPYRGYCPSHLEKLFTKPHLDIRGNGYYYPDNE
                     YVVAWYESDFIFKSRKAQAHAKVKLVNLTCPKYTGKNSAEYLSVLYISVPLNYLHYFT
                     IGSIWKGGVAKEQFALEEFYITVEAENQDGKRENLSIVSFGESEENGLEKPFDYTIYT
                     IPTEYHNYGDDLNRLLSISYQNQKFIIHPLHIFMMHYGYSTDIKRILATYPLDEVRER
                     LFIDKVVENFDVERYVVLPKYFVKKDAIFLYHFKYDEETTGTRVKKLVSQFRLNIHNQ
                     THPIEIDFWHTQKVELKIRGVRLGNAVLCAEIIGLNQPEGEDITLVLSQSKREKSGNK
                     TEEQNNEVNNVPITRVYTREPELDELPLTDNSPDNRTVEYNKRQFELLGRQRQIRALK
                     KAREESNQKMKALTPDEIDSLGVGESDGRNGKVGLAFCFLDDTPVGKSSKLYKLWQHA
                     KAVTEWNHGEAHWYTPNLGFRNDDELLPVSLETNKCFDYPEIAIIIRLQIYGETFFLI
                     DFSMKQRDISIRGLGYKPDPNEDFLYESDMSKSELSELLSAVHHHEHLPKEYIEKKNK
                     EKTKLTTFNHSEAESSNWAYNAVQKLTSRAITKPTYF"
     gene            16492..>19249
                     /locus_tag="PXE44_000934"
     CDS             16492..>19249
                     /locus_tag="PXE44_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDL5588460.1"
                     /translation="MNAEQRVIVTPTFWGKFFGKIKSVELQQNKVIVTDKKNNITEHD
                     LGKTFDFPAIQKSFFGTKLFFKDDSTEVVLSKLAKKQTDSLLLEIEKVVASNIKVKVK
                     EGFQHFANLAENQYLRDSDIPTLNDRVRLSVLSYGDNKEHFQKYFDESLVKKIQYISS
                     LFPVNAINAQTIRREYEQHVLNHRKHFFDVIESNPLTEQQRLSVIRNNDINMVLAAAG
                     TGKTSVMVAKALDLIDSGACKSEDILILAYNRDAATELSERLQECAREANLDLENSPS
                     ISTFHALGRRIIKECGKSVHLSKFAEDPMKLDRWFNKWLEDYIKEDPDNLAKFIQLAY
                     QPIDPFQFKTKDEYDRYVNDNDYRTLQGERVRGYQELLIANWLFSHSVEYEYEPRYVS
                     KRRIEIGFDYKPDFSLGDGVYLEHFGIDRQGRTRADINAQEYNANIQRKRELHAEHNT
                     TLLETYHYNWVENTLYKRLEQLMNEQFIPLKPKSQQEILDALNESGIFKENKNRYLKC
                     LQAIRTERLDYQQILKRLTDAKIVYAKEYATLLMRIHDAYVKELRSANEIDFDDMILL
                     ATQLVKTGEFKPKWKHILVDEFQDISMARLELLKEIYTKGPRPIWTVVGDDWQSIYRF
                     SGGKLEVTTRFNDMVGSHTLSKLEKTYRYNNSIADTAGQFIMQNPEQYQKNVVTHTKV
                     ADSCVHLYDSHVVKDEKSEANISLKASAILKLIRQKAPEATVAILARYRYLLEDAKSV
                     IKDKNVKFWTFHGSKGLEADYCILLGFFQGKVGFPNQNKEEAVVEALLPMLDSFPHSE
                     ERRLFYVALTRAKKESYLIADANAPSEFINELLSPKYKIDIVSKRFQERYRTIFKCNE
                     CSTGYFVMKQGKFGQFYSCTSGEVCRSSPRICEKCGRPMKLREGKFGQFWGCSGYGIK
                     GDQCT"
BASE COUNT         5777 a         3908 c         4112 g         5452 t
ORIGIN      
        1 cttgtctttc cgcaaaaaat ctgtataatt tgaccgcttg ttttctcttt tgaaacaagt
       61 tcgttaccct cacctcgaac gagggtttat tttttactat caacatttag aggaaaggtt
      121 atggtaacca ttcgtttaac tcgtggcgga gctaaaaaac gcccatttta ccaaatcgtg
      181 gtagtggaca gccgttcacc tcgtgatggt cgctttatcg agcgtatcgg tttcttcaac
      241 ccattagcag caggtcaagc tgagcgttta cgcttagatg tggctaaagt agatgcttgg
      301 gttgctaaag gtgctgattt atcagaccgt gttgcttctt tagtaaaaga agctcgcaaa
      361 gcagcttaat ttttaagcca aaattaaata agtaggtgag tatgagcgaa caaaagatcg
      421 aagttgtcgg aaaattaggt tcaacctatg gtatccgtgg ttggttgcgt ctctattcat
      481 cgacagaaca aactgagagc attttcgact accagccgtg gtttttaaaa attaaaggtc
      541 agtggcagca ggtcgaatta gaaagctggc gttatcatag caatgatttg attgttaaat
      601 tgaaaggcac tgaagaccgt gaagccgcac agttattaac caacgctgaa attggcgtgg
      661 atttgtctgt gtttcctgaa ttggaagagg gagattacta ttggcacgat ttgatcggct
      721 gttcggtagt aaacttagag ggctacacaa tgggaacagt tgccgaaatg atggaaacag
      781 gttcaaatga tgtgcttgtg gtgcgtaata acggcaaaga tgctttcggt aaacaagaac
      841 gattaattcc gtttttgtat gaacaagtag ttaaaagagt agatctcgcc accaaaacca
      901 ttacggtgga gtgggacgct ggtttctaac ctcaggtatg cggtgagctt tggaaaacat
      961 ttaaggcgtt aaaatgtgga ttggtattat ttcactgttt cctgaaatgt ttaaagcaat
     1021 taccgatttc ggggtgacag gacgtgcagt taaacaaaat ctactgcaag ttgaatgttg
     1081 gaatcctcgt gattttacat tcgataaaca taaaactgta gatgatcgcc cttatggcgg
     1141 tggtccggga atgttgatga tggtgcaacc tttgcgtgat gcgattcacg cagcgaaaaa
     1201 ggcggcatta gcagtagaag gcggtgaagc taaagtgatt tatctttcgc cgcaaggacg
     1261 taaactcgat caaaacggtg tgcaaacact ggcttcaaat cagaaactga ttttaatttg
     1321 tggacgatat gaaggcgtgg acgagcgatt gattcaaaca gaagttgatg aagaatggtc
     1381 aatcggtgat tacgttctca caggggggga attacctgca atgacattaa ttgatgcagt
     1441 tgcaaggttt gttcccgggg tattaggcaa acaggcttcc gcgttagagg attctttcgc
     1501 agagggatta ttagattgcc cacattatac ccgccctgaa gtattagacg gtatgcctgt
     1561 tccggaagtg ctgatgtctg gacaccatga acaaattcgt aaatggcggt tggaacaatc
     1621 gctggaacga acgtggttaa gacgccctga gctattggat agcctagctc tgactgacga
     1681 acagcgtgtg ttgttagcta aaattaaaaa acagcacaaa atcagttaat tctaggatta
     1741 tagaaggttt ttaaaatgag taacatcatc aaacaacttg aacaagaaca gttaaaacaa
     1801 gacttaccta gcttccgtcc gggtgacaca ttagaagtta aggtatgggt agtagaaggt
     1861 agtaaaaaac gtttgcaagc gttcgaaggc gtggttattg caattcgtaa ccgtggcttg
     1921 cactctgcat tcactctacg taaagtttca aatggcgtag gcgttgagcg tgtattccaa
     1981 actcactcac ctgtaattga cagcatttct gttaaacgta aaggtgcggt acgtaaagct
     2041 aaactttact acttacgtga gcgttctggt aaatctgcac gtatcaaaga gcgtcttggc
     2101 gactaattgc tacaaaacag tgaaatgcac aaaagccttg aggaaactca aggcttttgc
     2161 tttttgtact tacaagcagt tatttttcct caatgattta caaatcttgc tttctttttt
     2221 gttataatgt taacaagata acattaatga taggtgtcga tatgaaacaa ttaagtatta
     2281 gattgccaga cgatgaattt acttttatta aaacatttgc ttctgctcat aaactgactt
     2341 tacaaaattt tgtgatgtcc gcaatccaag ctaaagttgc agaaatgtct gctaaaaata
     2401 aaatatctga agaaagtaac attcatcaac ctctttctaa agatgaaaaa ataatgctaa
     2461 atgaggcaaa agtattttat tctgctcgtt cgcatttaaa ggcagaaaat gtgattcttc
     2521 cgagtgattt tatggagcaa attaaatcag gctgtggagc agaaaaaatg gcagaaatga
     2581 tgggatatac caaagaagat tgggagtcaa tgaagtaata tggggcagat tacatttagt
     2641 aatattttca caaaagtgat ttttgagaat gtgctttctg cttcaactaa agagaaaata
     2701 aaacaaatgc tttttcagtc tgtggttgca ttacctcctc tccattcaga aagaaaaatg
     2761 attttacagt tgaaaaagca aaaaattacc attttttatc aagttattga ggaacaatct
     2821 gtgcaaatcc tcgcttatca atttggtgga actgataaat tgacaggtgt gtctaaagct
     2881 cactttaaaa cactagatgc catttttcaa atgacggatg aagagaaaga aggaaaattc
     2941 taatgtaagc ggttattttt ccatattttt ttgcaaataa cgtttctcaa attttgcttg
     3001 atggcgttca agcacttccg tcatcgaaat gccatattta tcggctaata caaaaagatt
     3061 atccatcaca tcgcctaact cttcgattaa gttatctcgt ttttgctgtt gtgaagcttc
     3121 ggtttcgtcc ggtcgttcac gcccgatttc aatcgctctt actgcacaag ccacttcgcc
     3181 gatttcttct gctagataag tcacccgcat aaaagggctt cgttcatacc aaccttgttg
     3241 ttggtaaaac tggcgaaccc attgttgata ttgttcaatt tgcattttat ttctgattca
     3301 aaaagtaggg acgaaaaatt tttcgccctt ttataagaaa atgtatcagg gcaaatgatg
     3361 atttgcccct aggagtgtta ctctgcctca gagagaataa tccagccact atccagccag
     3421 tcgcaaatgg tgtctagcat tagctccagc tccgcttggt cttgcacttt ggaggcaagt
     3481 ttgttccacg aaatcgaatc tccattggcg atacgaatca gtaatgccat ttctgcttcg
     3541 tttaactcat caatccactc gccatttaca taaacccgtt gagggttctc ggtgtagagg
     3601 atttttacat ttgcatcttg ctgtaaccag ccgccggctt cgagaatttc ttgcacttcg
     3661 tcagggtaat attcattgtc agtttgcaat aattcataac ggcgggagct tgccgcagtg
     3721 gcaacactgt gagtgagtaa ctcatcgaac tgttcggaat tttggagcaa gtcgattaac
     3781 tgatgtttaa acattttcac cattttcgga tcaagcaacg cattcggctg ttcattcggc
     3841 gttaagcgga acggaagatg aaattccgat gaattaatgt tgtcggcttg ttgttcgatg
     3901 gttttgcaga gattttccag taaatcggtc gcatttggat aacgtaaacc aaaagagaaa
     3961 gttaaacctt gctcgctttc ggaaacacca tagtgagcca ttcgagacgg cacataaagc
     4021 acatcacccg ggttcattac ctcgtccatt accagttcgc ccatatcatc aaaaatgcgg
     4081 atcggttggt ttggtttgaa ttcggtgctt ggatcgcacc atttgcctaa ttgccaacga
     4141 cgttgcccat agccttgcac caagaacaca tcgtactcat cataatgttt gccgacagtg
     4201 ccgttgagcg gtgagcaaga aaccataatg tcatcacgtt gccattgcgg gataaaacca
     4261 aaagcctgcc aaagctcgcc tagctcagga gaccattgct cgaggttttg caccattaca
     4321 gaccattgta caggtaactc ttccaaatct tcctcaacaa gcgggctgga tttaacagac
     4381 catttctcgt tttcgcattg aatcaggcgt gcagtgactt cttcctctaa tgccagttct
     4441 aaaatatctt ccggctcaaa caatccgaca atttggggta agccgttacg aattaatagc
     4501 ggtcgttttt gccaatattc tcgcaaaaag gtttcagggc tgatattttc aggtaagcag
     4561 aatggaatgt tcataatggc tccttgctac gtgcttcttt gagattactg atattctacc
     4621 cgagaaatca ggaaaaacgg gaaatttttt atgatttagc gacatcattc acattttgaa
     4681 gaggaaataa tgaaaaaggc gaacattgtt cgcctttgaa atttatttta aaccgagttt
     4741 tttctcgaga tagtggatat tggtgccacc tttgcggaat tctttatccg caagaatttg
     4801 agtatgtaaa ggaatattgg ttttaatgcc ttccacaatg gtttcggaaa gggcattttc
     4861 catccggcga atcgcaatat cacgattttc agcaaaggta atcaatttac caatcattga
     4921 atcgtaatgt ggcggcacgc tatagcctga gtaaatatgt gaatcccaac gcacacctaa
     4981 accacccgga acgtgtaaac gggtaatttt acccggcgat ggcaagaagg tgtttggatc
     5041 ttctgcgttg atacggcact caatggcgtg acctttcact ttaatatcat cttgtgtgat
     5101 cgaaagtggt aagccggctg ccacacgcag ttgctctttt accaagtcca caccggtaat
     5161 catttcggtt acagggtgtt ctacctgtaa gcgggtgttc atttcaatga agtaaaactc
     5221 gccgttttca tataagaact caaaagtacc tgcaccacgg tagccaattt cacggcaggc
     5281 attggcacaa cgctccccaa taaattgacg caattcaggt gtaataccct gagcaggtgc
     5341 ttcttccacc actttttggt ggcgacgttg cattgaacag tcacgctctg ctaaataaac
     5401 cgcattaccg tgagtatcgg caagtacttg gatttcaata tggcgtggat tttctaaata
     5461 tttctccata tacaccatat cgttattaaa tgccgctttc gcttcggctt tcgtcattgc
     5521 gatagattct tccaaatctt tctcgtgatg aaccacacgc ataccacgac cgccaccacc
     5581 acctgatgct ttgatgatca ccgggtagcc gatacgttta gcgatttctt tatttttctt
     5641 cggatcgtta ccaaccggac cgtcagaacc cggtacgcaa ggcacgccgg cttttttcat
     5701 tgcattgata gcagagactt tatcgcccat taagcgaatc acatcagccg ttggaccaat
     5761 aaagataaag cctgatttct caacttgttc ggcaaagttt gcgttttcag ataagaaacc
     5821 gtagcccgga tgaatagcat cggcattcgt tacttcggca gcggcaataa tcatcgggat
     5881 atttaaatag ctttttaccg atggagcagg accgatacaa atggtttcat cggctaattt
     5941 aacgtgtttt aattcacggt cggcagttga atgcactgca accgttttaa tgcccaattc
     6001 tttacaagca cgcaaaatac gcagggcaat ttcaccacgg tttgcgataa caactttttc
     6061 taacataggg aaattccttg tgttctagaa aatgtagggc gtgttgcaca cgcccgttgg
     6121 ataatttaag gaagagctta ttcaatgatg aataattttt ggtcgaattc taccggttcg
     6181 ccatcattaa ctaagatttc ttttaccaca ccggctttgt ctgactcgat acggttcatc
     6241 attttcattg cttctacaat acataacgta tcaccgattt tcacggtttg acccacttca
     6301 acgaacggtt ttgcatctgg gcttgggcta cggtagaaag tccccaccat tggtgaaagc
     6361 actgcgtggc cactcgtttc tgcggctttt ggtgcttcag ccggagctgc aacaggtact
     6421 gtcgaagcta cttgaggtgc cgctggtgca acaacaggag ctgctgcata ttgaactgca
     6481 gctggtgcaa cagctggagc agcacggcta atacgcaccg taccttcttc ttcagacact
     6541 tctaactcgg taatgccaga ttcttcaact aattcaataa gttttttgat tttgcgaata
     6601 tccataataa gattccgtat gtgattaaat aaaataaaaa agagaactcc acatttgggg
     6661 tataaattca aatgtagagt taaaaaatgc tgggcgtaga atacctgaat ttcgctaatt
     6721 ttgcgaataa aatctgataa ttttggcgaa aatggacgat tttagcattc atcgtaccag
     6781 ttaggatttt tggaaaatgg tgactcacaa gcgattgaaa attgccaatt ttttgtaaaa
     6841 aacaaccgct tgtcagatta tttcaataaa tcttcatttt tcttgtaatt ccacttgcta
     6901 ccgggcttgt cgatatactg aaaatattcg ttggtaaaag taccttgagc acaagttact
     6961 ttgtctcggc ggaaatttac ttctttatca ccaatggcat tatttagggt gtcaggaccg
     7021 gtcataacaa acacgccacc ttcaatccga cgattctcaa tattgctgat aatcagctca
     7081 agggtgtctt ttagaatcca atttccagct tccgaagcca agaaaaagtt ggtgtattta
     7141 tcacggcgtt taattaacac ttcgctatca ttttcgccaa taattttaga gagtgggtaa
     7201 accaaatgcc cgtcaatatc aatataaatg ccaccttctc ggagcaaagt aaaaattcgc
     7261 caaaaatcag cttgtgccgc accatcggtt aatttgctat aggcgttaaa ggtgcgttca
     7321 tcggcgttgg ccttgatgta tgcctctcgt tcttctgtgc taacgtagcg ataatcgtag
     7381 ctcaatgaca ttaggcggtt aaacagataa ttcgcataca ttggcaaggt gactttgtta
     7441 gaataattcg tttgccaaat ggttttggta attttgctcg gaatgcttga ttggattttt
     7501 gccgggctat attccggaat ggtgaaacgt tttttcggaa aaagtgcatg gaacggataa
     7561 ctcaatattt tgacgatatt accgaataaa cggaagactc cgttacagat cacaataaat
     7621 tgttcatttt taacagccat tttattacct tatgctttta agtgtgaaat tgcaaaatcc
     7681 aacgcatatt catagccttt tgcccctaaa ccgcaaataa cgccttttgc aatatcactt
     7741 agataggagt gatggcgaaa aggttcacgg ctatgaacgt tagacaaatg cacctcaaca
     7801 aacggaatcg acacactcaa taacgcatca cgaattgcca cgctggtgtg agtaaatgcc
     7861 ccggggttaa taataataaa atcggtatgc tgaaaggctt ggtgaatacg gttgataatc
     7921 ggctcttcac cgttggcttg aaagctctcc agctcaacat cttgggccgc agccaatttt
     7981 tttaagtggt tttcaatatc cgccaaagtt tgcgaaccgt aaatttgtgg ctcacgtttg
     8041 cccaacatat ttaaattcgg gccgttaagg agaaggattt ttttcatatt tacttattca
     8101 acttgtaatt cataattaag ccattcacgg ttttctttcg cccccaagcg ttgataaaaa
     8161 cgaataccac cgtcattcca aggattttat tgtcttacaa tgatgtgcct tcgcctcatt
     8221 tttcaaggct tgcattaacg cctctcccac tttctgattc cgagcagttt catcaacata
     8281 aagctctttc atataaatag cggggcggtt ttgggcagtg taaggaagaa aataataaac
     8341 caatatgccg ataatgttag acgcattttc agccactaaa cagtaaaaat cgggtgggct
     8401 tttgcgaaag ccggattcca ttacaacttc aggagtaatc gcaaaagaat cgatatagtg
     8461 ctcaaacaca gccagttttt gcatcagagg gaacacttgg tgggtatctt gttcgaccat
     8521 tttgcggatt gtaaatgcca taaaattcct tacaagcggt aaaaaaatcc ctcacttttg
     8581 caaagcgagg gaggttttaa ttattcgtca atagaacgta ataagtcgtt taagcccact
     8641 ttgcttaagg ttttctcatc cacttttttc acaatcaccg cacagtagag gttgtgtgaa
     8701 ccatcttttg atggaagcga gcctgaaacc acaacagagc ctgccggcac acggccgtaa
     8761 tgtacttcgc cggtttcacg gtcgtaaatt ttggtagatt gaccgataaa cacgcccatt
     8821 gagatcacgc agttatcttc gacaatcacg ccttctacga tttcagaacg tgcaccgata
     8881 aagcagttgt cgccgatgat ggttgggttc gcttgtaatg gctctaacac accaccgatg
     8941 cccacgccac cagaaaggtg aacattttta ccgatttgcg cacaagaacc cactgttacc
     9001 caagtgtcca ccattgtgcc ttcgccaacg tatgcaccga tgttcacgaa agatggcatt
     9061 aacaccacgt ttttctcgat atgtgaacct ttacgcacca ccgcacccgg cacggcacga
     9121 atgccatctg ctttgaattg ctcttcggtg tagttgccgt atttggttgg cactttgtcg
     9181 tagaatttgg tttctgcacc gtcgatgatt tcattgtcgg caatacggaa tgaaagtaat
     9241 accgcttttt tcacccattg gtttactacc cactcgccat cttttttctc ggcaacacgc
     9301 agtgaaccgt tgtctaatcc tgcgatcacc tcttctactg ctgctttggt ttcagcagca
     9361 acggttttcg gagtaatttc cgcacggcgt tcaaatgccg cttcaataat agcttgtaaa
     9421 gacattgttg tgttccttca atatataaat taaaacagaa aattcggctc actttttaac
     9481 atagaaaggc gtttatttcc acaagaaaaa acatatcgca gaaatttagt ttataattcc
     9541 aagccactta tttgatacaa gcggtgattt ttgtggaaaa ttttgcaaat ctgcttaccg
     9601 gtaaaacgcg tgagcattta gtgccgttgc cgaacgctct ttcggataaa cattaccttc
     9661 agcctcaggt ggttgaggct tttttgacgt tgcagcatgc tgcaaaacaa gccggcttta
     9721 atttacagcc tgccagtact tttcgtgatt ttgaacgcca aaaagggatt tggaatgcga
     9781 agtttagggg tgagcgtaag gttcacgatg ataacggggt tgcgattgat atggcggcgt
     9841 tatcggatct tgaaaagtgc aaagcaatgt tgagatggtc tgcggtgccg ggggcaagtc
     9901 ggcatcattg gggaacggaa attgatattt ttgaccctga tttattgccg gaaaatacgc
     9961 ctttattgct tgagccgtgg gaatatttgg aaggtggcta ttttgccgat ttaaccaagt
    10021 ggttacacgt taatgcggaa acatttggct tttatttccc gtttgacggt aaacattctc
    10081 gagtgggctt tgagccgtgg cacattagct atcgcccgat ttcaaatgaa tatgagcaac
    10141 gctttaacga tgaaatctta aaagctgcgt ggcaagatga agaagtggcg ggtaaggaat
    10201 gcttacttga acattttgaa gaattactaa aatgatccaa ttaattaacg aaacccaaca
    10261 acacgacaaa ttccgccaaa tttgcgacaa atggcagctg gttgaagata aatccagccc
    10321 gttagcctta gtgctaacgg aggaacgatt ggaactacgc aaattagacg aacccaagct
    10381 cggggcgatt gcagtgaatt ttgtggacgg tacgatggca catcggcgta aattcggtgg
    10441 tgggcgaggt gaagccgttg cgaaagcggt cggcattaaa ggggattatt tgccaactgt
    10501 gattgatgcg acagccggtt tagggcgaga tgcttttgta ttagcagcgg ttggttgtaa
    10561 agtgctgtta gtggaacgca atccaattgt cgctgccttg ttggaagatg gtttaaaccg
    10621 agcttatcaa gatgtggaaa tcggcagttt tatgcaagaa agaatgatct tagccgatgt
    10681 tcgagacatt tctcagctag atccacaatc ccaaatggct gaggtagtgt atttagatcc
    10741 gatgtatccg cacaaacaaa aaagtgcgtt ggtgaaaaaa gaaatgcggg tgtttcaaca
    10801 tttagttggg gcagatttag attcggacag ctttttctta ccggcaaaag gattggcaaa
    10861 aaaacgtgtg gtagtgaagc gtccggacta tgccccgttt ttagcggagc aaaagcctga
    10921 tttttcacac accaccaaaa atcaccgttt tgatgtttat ctttctcacc tgaaaaatga
    10981 ttaaaggtgg gtcatgcgat aggcaagcgg ttatttttac agtaaaattt acaaattatg
    11041 ttatcgttta tttatattta aaaaggcggt tatcgtgcca caacgtgatt atgtcgctcg
    11101 ttccaacgag aaaaaaagta aaaaaaatca aaaagaaagt aatgttaaaa agattttaat
    11161 gcggattatc ggggtaattt tattcttcgg tattggttta tggtttttga aatccagtgc
    11221 acctgtacca acagttccgt cagcaccgca gcctgctaat tcacagacta aaagcgaatt
    11281 accaagtcga ccggaagaga cgtggagcta tattcgtgat ttagaaaccc gtacggtaat
    11341 taccgataat agccaaactt cacaagaacg tttggcacaa ttaacggcgg aacaaaagca
    11401 gcaaattgaa gaaagacgca aacaagaaga agcacgctta gccgctgagc aagctcaaca
    11461 aaagcctgcg accgatgtat ctgctacaga attggctccg aaaacagagg aagttaagtc
    11521 gttaacggaa gaagaattag cagcaaaaaa agcacaggag caagctttag cacaaaagca
    11581 agcggaagag aaacgcagac aagcagagct taaaaaacag caggaagcga aaaaagagca
    11641 gccggctaaa attgtagcaa gtgctgcaac actggcagat aaagctgcaa atcaggcggg
    11701 taaatttggg ttacagtgcg gtgcatttaa aaataaagcc caagccgaaa atatgcaagc
    11761 aagattggca atggcaggtt ttaacgcccg tatcgcaagc agtgccgatt ggaaccgtgt
    11821 ttttgtcggc ccgataggtg acagagcggc aacttccaga gctcaatcca atgcacgcag
    11881 tgtagcggaa tgtgtgattg tggcgatgta aaattgattg aaatattgaa gagagctata
    11941 ggctctcttt ttttatatcc caatcgcata tttcaaggct tgctttttga tgggtgacac
    12001 tttatcagcc aatactaaac cgacattgcg ggcaattttg agcggtaata aatcggtctt
    12061 aaacgcttta taaaacacgt ccatgcctgt ttgcatcagt aaattatccg gtttacgttt
    12121 ttgctcatag cgttgaagca cctgctcact tgcaaaattt ttgccttttt tgaccgcttg
    12181 ttcgatcacc tcaagcaagg ttttaacatc tttaaagcct aaatttacac cttgtccggc
    12241 aagcggatta atggtgtgag ccgcatcgcc gactaatacc accccgtttt gcacatagtg
    12301 ctgagcgtgc tggcgggtta gcgggaaagc ccctgaagcc tgcacctgaa tgttgcctaa
    12361 ttctgcgttg agccggcttg ggaaatgttg ctcaatcgca actcccagct tttctgctgg
    12421 aagcattttc agttcggcaa ttttttgtgg tgaatcgtac caaacaacgc agccgtgatt
    12481 gccggaaagc ggtaaaaagg ctcgagggcc ggttggatag aattgttgcc aagtgccgct
    12541 tggagcaggt ttttcggtgg taacggtggc gagtaaacag tgttggcgat attgccaagc
    12601 ggttaaacca atgcccgccc attggcgaac ctgtgaattt gcaccgtctg cggcaatcag
    12661 cagtggggca gcaaaggtgt tgccatcaac cgtcacttcc caatactcgc ccgctttatt
    12721 tgcaaaaatt tgctcaaatc cgaccgcttg taggcagttt ggatattgct ttaattgctc
    12781 ccataacgca atttgaataa cgttgttttc gaccatatcg ccgagctttt ccaaacccaa
    12841 atcagctgcc gaaaaaagag tgttgaatcc atcaatttcc caggtttcga gctgggtata
    12901 aggggtgctt ctcattgcaa caatttgctc ccacacgccc aatttttcta acagttgaat
    12961 cgagcccata ctaatggcgg aaattcgcaa atcgtagtct gaatcaggat taaaggtcgg
    13021 cagagggttt ttctccaaca aggctaaacg cagcccctgt tttgctaagc ctagagctgt
    13081 tgctgcccca atcataccgc caccgattac aatcatatct aattcagttt tcattttttc
    13141 ttccttttta gttaggcaaa atactgctaa aacgccttga attgtagtgc atttcagatt
    13201 aggatcaaac cgatttatct atttatctaa ggagcgtttt atgtcttgca aaactcatgc
    13261 aaaccacgag cacgaacacg gcgaaggctg tggtcatact gctattaaac ataacgacca
    13321 tatcgactat ttacacgatg ggcatttaca ccacaacaaa cacggcgatc actatgacga
    13381 acacgtttta gaagtgaatg aaaccaaccc ggcagactgt aatgccgccc attgctgcgg
    13441 cgaccacgtt catggtgagg tttgcggaca cgaagctgtg ccgcacggcg atcatatcga
    13501 ctacatcgtg aatgggcgtt tgcaccacca acacggcgac cactgcgatg accacgggcc
    13561 ggttgaaatt gtgaaataag atttttgatg ggaggtttta gcctcccatt attatttttg
    13621 cgatctcgct cgaaaaattg aacattcctg ctacccctct tccgattttc cttttatgct
    13681 ctcggtattg atttctaata ccgaaatgtg atgaccttag cgattgttta cagccgagcc
    13741 tccattggcg ttgaagcccc gttggtgacg attgaagtgc atattagcaa cggacaacca
    13801 aaattaacca ttgtcggctt gcccgaagcc acagtgaaag aggctggtga tcgtgtgcgg
    13861 agtgcgttgc tgaacgctaa ttttatctat ccgccacaac ggatcacgat taatcttgct
    13921 ccggcagatt tacccaaaga aggcggacgt tttgacttgc cgattgcgat tggcattctg
    13981 gcagcctcag ggcaaattga ttctgattta ttaacgcagt tcgagttttt gggcgagttg
    14041 gcactgacag ggcatttgcg aggagtgcac ggcacgattc cggcagtgat ttctgccgat
    14101 aaagccaaac ggcagatgat cttagccaag caaaatgcga atgaggcatc gcttgtttcc
    14161 aatgccacca cctactttgc cggctcgttg ttggaggtgg taaatatgct caacaaacgg
    14221 gataaactgc cgatttgtca gcacatttcg caacattctg ccgaaattcg accgcttgtc
    14281 agccgagatc ttactgatat tatcgggcaa caacacgcca agcgagcctt aatgattgcc
    14341 gctgccgggc aacataattt gctgtttctc ggaccaccgg gaacgggtaa aacaatgttg
    14401 gcaagccggc tggcggattt actgccggaa atgaccgatg aggaggcgat tgaaaccgcc
    14461 tcggtgacca gccttgtgca aaatgagctg aattttcaga actggaaaca acgcccgttt
    14521 cgttcgcccc atcacagtgc atcaatggtg gcattagtgg gaggtggctg tgaaaactag
    14581 aaataaatgt caaaaacaag aaataaatgt cacaaatctt tctctaattt cgctataatt
    14641 cgatcatcaa aactttaagg ggaaatccgt tatgttctac ctcaataacg acaatttcaa
    14701 acgagaacct tatcgtggat attgtccgag tcaccttgaa aagcttttta ctaaaccaca
    14761 ccttgatatt agaggtaacg gttattatta ccctgacaat gaatatgttg tagcttggta
    14821 tgaatcggac ttcattttca aatcaagaaa agctcaagca catgcaaaag taaagcttgt
    14881 taatttaact tgccccaaat acactggcaa aaacagtgct gaatatttaa gcgttttgta
    14941 tatctccgtt ccccttaatt atctacatta tttcactatc ggcagtatct ggaaaggcgg
    15001 tgtcgccaaa gagcagtttg ctctcgaaga attttacatt acggttgaag ctgaaaatca
    15061 agatggtaaa cgagaaaacc tctcaatcgt ttcattcggt gagagtgagg aaaacggttt
    15121 agagaaacca tttgattaca caatctacac tatccccaca gaatatcaca actacggtga
    15181 tgacctaaac cgactacttt ccatctctta ccaaaatcaa aaattcatta tccatccgct
    15241 acatattttt atgatgcact acgggtactc tacggacatc aagcgtatct tggcaacata
    15301 cccacttgac gaggtaagag aacgcctatt tatagataag gtcgttgaga attttgatgt
    15361 agagcgttat gtcgttttac cgaaatactt tgtaaaaaaa gatgcgattt tcctctatca
    15421 cttcaaatat gacgaagaga ctacgggaac aagggtaaaa aagcttgtaa gccagtttag
    15481 gttaaatatt cacaatcaaa cacatccaat agaaatagat ttttggcaca cgcaaaaggt
    15541 tgaattgaaa atccgtggtg ttcgacttgg aaatgctgtg ttatgcgcag aaattatcgg
    15601 tcttaatcag cccgaaggcg aagatattac gctagtgcta tctcaatcga aaagagaaaa
    15661 atcgggtaat aaaaccgagg aacaaaataa cgaggtaaat aacgttccta ttaccagagt
    15721 ttatacacga gaacctgaat tggatgagtt accactcacc gataactccc cagataatcg
    15781 aacagtcgaa tacaacaagc gacaatttga actcttgggc cgtcaacgcc aaatcagagc
    15841 gctcaagaaa gcacgcgaag agagtaatca gaaaatgaaa gccttgactc cagatgagat
    15901 agactcactg ggtgtaggtg aatcagatgg ccgaaacggt aaggttggac tcgctttctg
    15961 tttccttgat gatacacctg ttggcaaaag ctccaaacta tacaaattat ggcagcacgc
    16021 gaaggctgtt acggaatgga atcacggtga ggcgcactgg tacacgccta atctcggttt
    16081 tagaaatgac gatgaactcc ttcccgtttc cttagaaacc aacaaatgtt tcgactaccc
    16141 tgaaatcgcg ataattatcc gcctccaaat atacggagaa acatttttcc tgattgattt
    16201 ctcaatgaag caaagagata taagcattcg tggcttaggg tataaacccg atccaaacga
    16261 agattttctc tatgaaagtg atatgagcaa aagcgaatta tccgaattat tgtccgcagt
    16321 tcatcatcat gaacatctgc caaaagagta catagagaaa aagaacaaag aaaaaaccaa
    16381 actcactaca tttaaccata gcgaagccga aagtagtaac tgggcttata acgcggtgca
    16441 aaaattaact tcaagggcaa ttactaagcc gacatatttt taattatttt tatgaatgca
    16501 gaacaacgtg taattgttac tccgacattt tggggtaaat tttttggaaa aattaaaagt
    16561 gttgaacttc aacaaaacaa agttatcgta actgacaaaa agaataatat aactgaacat
    16621 gatttaggaa aaacttttga ctttccagct atccagaagt ctttctttgg tactaaatta
    16681 ttttttaaag acgatagcac agaagttgtt ttatcaaaac ttgcgaaaaa acaaactgat
    16741 agtctattac ttgaaatcga aaaagtagtt gcatctaata ttaaagtgaa agtaaaagag
    16801 ggttttcaac actttgcaaa tttagccgaa aatcaatatc tcagagattc cgacattcct
    16861 acattaaatg atcgtgtaag attgtcagtg ctttcctatg gggataacaa agaacatttt
    16921 caaaaatact ttgatgaaag tttggttaag aaaattcaat atatctcttc actttttcct
    16981 gtaaatgcca taaatgctca aacaattcga agagaatacg agcaacatgt tttaaatcat
    17041 cgcaagcatt tctttgatgt cattgaatct aatccgctta ctgagcaaca aagattgtca
    17101 gtcattcgga acaacgacat caatatggtt cttgctgccg cggggacagg aaaaacgtct
    17161 gtaatggtcg caaaagcatt ggacttaatt gattctggtg cttgtaaaag cgaagatatt
    17221 ttgattcttg catacaaccg cgatgcagca accgaactat ccgaacgctt acaagaatgt
    17281 gcacgagaag ccaatttaga tctggaaaat agccctagca tctcgacttt ccatgcgctt
    17341 ggccgaagaa ttattaaaga atgtggaaaa tcagtccact tgtcaaaatt tgccgaagat
    17401 ccgatgaaat tagatagatg gttcaacaaa tggctagagg actacatcaa agaagatcct
    17461 gataatcttg caaaattcat tcaacttgca taccaaccta tcgatccatt ccaattcaaa
    17521 acgaaagacg aatacgatag atatgtaaat gacaatgact atcgaacctt acaaggcgaa
    17581 cgcgttcgtg gttatcaaga gttattgatc gcgaactggc tttttagcca cagcgtagaa
    17641 tatgagtatg aaccaaggta tgtatcaaaa cgccgtatcg agattggttt tgactacaaa
    17701 cccgatttct ctttgggtga tggtgtttac cttgaacatt tcggcattga ccgacaaggt
    17761 agaactcgcg cagatattaa cgcgcaggaa tataatgcca atatccaaag aaaacgtgag
    17821 ttacacgccg aacataatac aacattactg gagacatacc actacaactg ggtggaaaat
    17881 acgttatata aacgactaga gcaattgatg aatgaacagt tcattcctct gaagccaaaa
    17941 tcacaacaag agattttaga tgcactcaat gaatcaggaa tcttcaaaga aaacaaaaat
    18001 cgctatttga agtgccttca agctattcgt acagagcgat tagactatca acaaatctta
    18061 aaacgtctaa ccgatgcgaa aattgtctat gctaaagaat atgcgacatt gctcatgcgt
    18121 attcatgatg cctacgttaa agaactacgt agcgcaaatg aaatcgactt tgacgatatg
    18181 attttgttgg ctactcaact ggtgaaaaca ggtgagttta aacctaaatg gaaacatatt
    18241 ttagtcgatg aatttcaaga tatttctatg gctcgattgg agttattgaa agaaatttat
    18301 actaaagggc ctcgaccgat ttggactgtt gttggagatg actggcaatc aatctatcgt
    18361 ttctctggcg gtaaactaga agttactacg cgatttaatg atatggtcgg ctcacacacg
    18421 ctaagtaaac tagaaaaaac ctaccgctat aacaatagta ttgctgacac agcaggtcaa
    18481 ttcattatgc aaaatcctga gcagtatcag aaaaatgttg taacgcatac aaaggtcgct
    18541 gattcttgtg ttcatttgta tgactctcat gtcgtaaagg atgaaaaatc agaagccaat
    18601 atcagtttaa aagcctcagc aatattaaaa ctgatacgtc aaaaagcacc tgaagccact
    18661 gtcgcaattt tggctagata ccgttacctt ttagaagatg caaaaagtgt aatcaaagat
    18721 aagaatgtta aattttggac gttccacggc tcaaaagggt tggaagcaga ttactgcatt
    18781 ttacttggtt tcttccaagg aaaagtcgga tttcccaacc aaaataaaga agaagccgtt
    18841 gtagaagcat tgcttcctat gctagacagt tttccacact cagaagaacg taggttattt
    18901 tatgtggcat taacgcgtgc gaaaaaagaa agttatttga tcgcagatgc gaacgcgcca
    18961 tcagaattta tcaatgaatt attatcaccg aaatacaaga tagatattgt gtcaaaacga
    19021 ttccaagaac gttatcggac aatctttaaa tgtaatgaat gtagcactgg ctatttcgtg
    19081 atgaaacaag gtaaatttgg tcagttctat tcttgtacat caggggaagt atgccgctct
    19141 tcgccacgga tttgtgaaaa atgtggccgt cctatgaagc tgcgagaagg taaatttggt
    19201 cagttttggg gatgctcagg atatggaatc aaaggcgatc aatgtacga
//
LOCUS       DANLZC010000034        19150 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.034, whole genome shotgun sequence.
ACCESSION   DANLZC010000034 DANLZC010000000
VERSION     DANLZC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19150
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.034"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(74..745)
                     /locus_tag="PXE44_000935"
     CDS             complement(74..745)
                     /locus_tag="PXE44_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL5588461.1"
                     /translation="MLQAIIFDMDGVIVDTEYLEFSLQKQFIEDIKEHSRPITLEQQS
                     EVVGKCLKEIPVIIKKLSESSLPIEEIRARYYAFFQDLFSKVDFKTIFRADIQQIIQF
                     AKQNQIKLAVASSSALSHIENILTVCGIKDEFDLIVSGEQFEHSKPDPTIYRYTCEKL
                     GVEPQNAVAIEDSYYGMLAAKTAGLTVIGYEEKRILIDQSLADYMGKDMKEILKILQK
                     CNTPS"
     gene            complement(866..2329)
                     /gene="guaB"
                     /locus_tag="PXE44_000936"
     CDS             complement(866..2329)
                     /gene="guaB"
                     /locus_tag="PXE44_000936"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL5588462.1"
                     /translation="MLRIKQEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTETKLAISLAQEGGIGFIHKNMSIERQADRVRKVKKFESGIVSEPVTISP
                     DMTLAELAELVKKNGFAGYPVIDENQNLVGIITGRDTRFVTDLSKTVREFMTPKDRLV
                     TVKENASREEIFHLMHEHRVEKVLVVNNEFQLKGMITLKDYQKAESKPNACKDEFGRL
                     RVGAAVGAGPGNEERIDALVKAGVDVLLIDSSHGHSEGVLQRVRETRAKYPDLPIVAG
                     NIATAEGAIALADAGASAVKVGIGPGSICTTRIVTGVGVPQITAIAEAAAALKERGIP
                     VIADGGIRYSGDISKAIAAGASCVMVGSMFAGTEEAPGEIELYQGRAFKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRIAYKGYLKEIIHQQMGGLRSCMGLTGCATI
                     EELRTKAEFVRISGAGIKESHVHDVTITKEAPNYRMG"
     gene            complement(2557..5724)
                     /locus_tag="PXE44_000937"
     CDS             complement(2557..5724)
                     /locus_tag="PXE44_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDL5588463.1"
                     /translation="MKFTDIFIKRPVLAVCISLLITILGLQAIEKLQVREYPEMTVSI
                     VTVSVNYSGADARLMQTLVTSQLEEAVAQADNIDYMTSSSSPSTTTVTAKMKLNTDPN
                     AALSDISAKVNAVRSNLPSGIDDPSIRVSTGSNDALMYIRFVSDELNTSQVTDYINRV
                     VKPQFFTVNGVSSVDVYGSVFGLRIWLDPDKMASNNLSGAAVLSALSANNLQTAAGNS
                     NGYYTVYRNKVLSTTPSVTELEEVTVSTTKDGKTIKLKDIATVELDKYQDAARATVGG
                     KDAVVLAVSVAATANSLTVAKDIYPMFEQVLKNLPSTIQGDIVYDKTIAIDSSIKEVI
                     KTIGEATVIVLVVIILFLGSLRAMIVPVITIPISLIGVLFFLQMFGFSINLLTLLALV
                     LAIGLVVDDAIVVLENVERHVKEGKTPFDAAIIGTREIALPVISMTITLGAVYSPMAL
                     MTGVTGTLFKEFALTLAGAVFISGIAALTLSPMMSSRILKEHKEGEESAFARVVHRSL
                     DKMTSCYTNMLSGIMAVRGFVLFFAVLIFASLPMLFGSLSSEVAPTEDRGFVVGISNG
                     PSNTNLDYTEAALAQFNQEVTKLPEVSSLMTVSGFNGANSALSIIALKDWNDRSRERA
                     AISNDVAAIGKKVVGMDVNAIAFPEISTGENGLPFSLVITTADSYEKLAIVASEFLKK
                     AQTSGQFFFANLDLKFDTVTMNMKFDREKMGAYGVTMQQVSGTLGAFLSGAIITRVDI
                     DSRAYPIVSQAVRNDRLNPEDLMNYYVTTATGQSIPLGSFVTMELKTSPSSLPRMSQL
                     NSATIGGVVSGSIGDAVNWAKAELDRTLPSGYQYDFRGESRQFVQEGNAMAMTFALAV
                     IIIYLVLAIQFESWRDPLVILVSVPLAVSGALLVMNIPTAMKGLFMGIAAGTGSKDMM
                     AFANGIWTAGYTLNIYSQVGLITLVGLITKHGILMCEVAKEEQLLHGKSRYEAIFHAA
                     TIRLRPIMMTTAAMIAGLIPLLFAVGAGAIARFSMGMVIVAGLAIGTLFTLFILPVIY
                     TFLGQEHKPLREFDEEKYFPTTKEA"
     gene            complement(5738..6943)
                     /locus_tag="PXE44_000938"
     CDS             complement(5738..6943)
                     /locus_tag="PXE44_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL5588464.1"
                     /translation="MTTETQKLGKGRKFLIVLTLLIVLIVFAAIVGLQKIGAMKKAEA
                     AANMPEAVSEVTAMQVTTQEWTPTISAVGYIRPNQGAMLSAETSGVVSRVLVTSGQRV
                     KKGDLLVEFDSSVEIANLRASEAQLPNAKANLERYRNLIASNSASKAEFDNAQSTYNQ
                     LLANIEALRATIKRRQIYAPFSGLAGIVNVNVGQYITMGTEIVRVEDQSSMKVRFTLP
                     QTNLEQISIGQKVTAVIDALPAQTFPAKIVAIDPAVDRLTGLINVEAVIEEGQEKLLS
                     GMFARLNVALPTETGQIVVPQISIAYTMYGETVYVLQPLSDEDKEMVNKMAVQNPSLD
                     VNKMYRAKQMEVKTADRKGNYSQLSKGVKAGDLIVTGGLQRLSNNALVKVSDTEAVGI
                     TTPAKNSKL"
     gene            complement(6974..7546)
                     /locus_tag="PXE44_000939"
     CDS             complement(6974..7546)
                     /locus_tag="PXE44_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL5588465.1"
                     /translation="MSRRSEPKEEMVNRILSATESLIVQEGLQNLSMRSIAKEAGIAS
                     GTLYLYFKTKDDLLHSLAAQLLECYNGYMHFEFKPEMALFEQYKALVNKKWQFLIERL
                     DLVKQFQAVIGIDELIRETIADETSFWNKLILEGKKQRIIADLSNELLYGLSIGTLVD
                     IQYLQNLNQENQFDGYLDEIILRTWKAITF"
     gene            7785..8006
                     /locus_tag="PXE44_000940"
     CDS             7785..8006
                     /locus_tag="PXE44_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1414 domain-containing protein"
                     /protein_id="HDL5588466.1"
                     /translation="MATKSKYHTKQFEQLLADLIATIEKHKAPVDLSLMALGNMITNI
                     LIENIQSPAQREALADAFADALKQSLKTK"
     gene            8011..9744
                     /locus_tag="PXE44_000941"
     CDS             8011..9744
                     /locus_tag="PXE44_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HDL5588467.1"
                     /translation="MFARLRSLLPTNSRQYREETSQKITWGHWFALFNIVLALLISSR
                     YAFNADWPNTLVGKLYFFVSLFGHFSFVVFAGFLLILFPLSFIVKNHRTYRGLSVILA
                     TIGQTVLLVDTEVFKQFYLHLSPLVWDLLVNPEEGELTRKWQLLFVPMPLLLLAEMLY
                     SRWCWQKLRSFSRQKWGKYVALFFVISFTATHLIYAWADMNAYRPITAQRANYPLSYP
                     MTARNFLEKHGFIDRVEFNQEIEESGRLDTFYLNYPKSPLIATKTPSTNILLINLSGL
                     SKQIINAETMPKLTEISQQSHRFMQHYSSGDSASAGALGLFYSLSGKYLDSVLADKTV
                     SPLIEIARKSDYQLGLFSHNGFADPIYHQAIFSGILLPTAHSNVGAIGQWKNWISSRS
                     LENPFFSYLDLRMPFAHTAQEQAKLLDWQFAEIWQHLTQNALLANTLVIITADISNEV
                     GAENSFDKQHIQVPMMFYWQGESKEYHFLSSHLDIMPTLLSQFFGVQNPLNDYAQGMD
                     LSTQNQRLWVSSSNHKWNVAIMPNGDQYQIDRKGNFKHFNSNGEHEKDARPPLALFLQ
                     MIQQSNQFIEK"
     gene            complement(9811..10512)
                     /locus_tag="PXE44_000942"
     CDS             complement(9811..10512)
                     /locus_tag="PXE44_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5588468.1"
                     /translation="MIGQALGWRATFGIIGIIALIVLMMIWRLLPYLPSKNAGSIKSL
                     PLLFKRPMLIGIYLLVALIVSGHFTAYSYIEPFMIKISSVSVENTTFILLVFGLAGVV
                     ASFLFGRLYGKAPNKFILSGIAIIMCSQLCLLFLSDSTVAMFMVAFVWGIGITGMSIA
                     LQMKVLELAPDATDVATAIFSGTYNLGIGGGALLGSIAIQQLGLANIGFAGGSIAFVA
                     MVWFVFVSLKYRQLR"
     gene            complement(10509..10889)
                     /locus_tag="PXE44_000943"
     CDS             complement(10509..10889)
                     /locus_tag="PXE44_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5588469.1"
                     /translation="METLAIFLFSEIWKFDFVFTFIFKILKINVFFSDLINMKLARKA
                     QRRLSFLRVFSLAFAAFIVNTTEFIPIALLSDIASSFQMEPSSAGIMMTVYAWVVFLL
                     SLPLMLLTANMERKRLLLMLFMGE"
     gene            complement(10931..15391)
                     /gene="mukB"
                     /locus_tag="PXE44_000944"
     CDS             complement(10931..15391)
                     /gene="mukB"
                     /locus_tag="PXE44_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDL5588470.1"
                     /translation="MIDTTELQNNPQIAPLARPNDVQRGKFLSLTLINWNGFFARTFD
                     LDDLVTTLSGGNGAGKSTTMAGFVTALIPDLTLLNFRNTTEAGSTAGSRDKGLYGKLK
                     AGVCYAVLESFNSRGQRIITGVRLQQIVGRDKKVDIRSFSLQNIPQEQSIISLLTEQV
                     GNKARVLALNELKDQFENSEVQFKQYHSITDYHSFMFDLGVIPKRLRSAADRSKFYKL
                     IEASLYGGISSVITKSLRDYLLPENTGVRQAFQDMESALRENRMTLEAIKVTQSDRDM
                     FKRLITESTNYVSADYMRNANERRGNVQQALEQRKEWYAAKSKILLEQQRFVEFSRES
                     ADIAEAELALEADYNSANDHLNLVMNALRHQEKIERYQDEVEELNIKLEEQQEALEEI
                     AEIAENAQARADEADDHVEELRSQMADYQQALDAQQTRALQYQQAVNALEKAKQLTGL
                     VNLDLNNIEDYHAEFVAQAEDLTDQVFELEQRLSVSDMAKTQFEKAFELVCKISGEID
                     RLQAWEEARALLSAFPEQKMQAQQAVSLRQKLNDLEQRLQQQQNAQRLVAEFNQKSQQ
                     QLNSAEELDAYFEEQQARLEDLEAELSDFVEQRSTQRQQREQLNQQYQQLAKNAPAWH
                     TAQSALTRLEEQCGEKFEASQSVMQFMQNMLSKEREATLARDELARKEQMLDEQINRL
                     SQPDGSEDARLNQLAERFGGVLLSELYDDVSIEDAPYFSALYGEARHAIVVRDLEAVK
                     TQLEKLDDCPSDLYLIEGDPSAFDDAVFSAEELGEGVVVKVSDRQWRYSKFPEVPLFG
                     RAAREKHLATLKAERDETAEKHAERAFDVQKCQRLHQHLSQFIGTHLNLAFQENPEML
                     MQEIAAERAEIERELAQSAGNEQQLRHQLESVKAQLQMLNKILPLANLLADETLEARA
                     EECREQLELAEEDEMFIRQFGATLSQLEPIAAALKSDPALFEQLEADYRRSVAEQKVL
                     QQKVFSLADVMNRRLHFSYQETVGTEGSALTEQLRQRLENAQREREQARDQLRQAQAQ
                     LSQYNQVLTGLRSSYEAKNQMLQELIREIDDLGVRGDSGAEERARFRRDELQQRLSQQ
                     RSRKGYLDKQIAVIEAEMDNLNRTLRKAERDYHTQRELVVQAKASWCIVQRLSRNSDV
                     EKRLNRRELAYQSAEELRSISDKALGALRTAVADNEYLRDSLRASEDSRKPENKVAFF
                     IAVYQHLRERIRQDIIKTDDPIDAIEQMEIELSRLTNELTGREKKLAISAESVANILR
                     KTIQREQQRILQLNQGLQNIAFGQVKGVRLVVNIRDTHAILLNALSNNREEHKDLFDS
                     QKLSFSEALAMLYKRVNPHIEMGQRTPQTIGEELLDYRNYLDLEVETFRGADGWMRAE
                     SSALSTGEAIGTGMSILLMVVQSWEEEARRMRAKDILPARLLFLDEAARLDATSINTL
                     FELCERLDMQLLIAAPENISPERGTTYKLVRKITNNQEYVHVVGLKGFAK"
     gene            complement(15567..16283)
                     /gene="mukE"
                     /locus_tag="PXE44_000945"
     CDS             complement(15567..16283)
                     /gene="mukE"
                     /locus_tag="PXE44_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDL5588471.1"
                     /translation="MTENIQDFISPKLALAIANPIFPELDSQLRAGRHINTEQLDQHV
                     FLMDFQAELESFYRRYHVELIRAPEGFFYLRPKASTLIARSAMSEMEMLVGKVLCYLY
                     LSPERLAQQGIFSQDDVYEELLSLADESKLLKAVNPRSTGSDLDRAKLAEKAGGALRR
                     LARIGIITRVGEQNSKKFVISESVFRFGADVRTGDDPREAQLRLIRDGEATTPALLNE
                     QAVEIDENIADEAPFDGENE"
     gene            complement(16332..17675)
                     /gene="mukF"
                     /locus_tag="PXE44_000946"
     CDS             complement(16332..17675)
                     /gene="mukF"
                     /locus_tag="PXE44_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDL5588472.1"
                     /translation="MQNELAQTIPELISWTKDREFSLSLSSDRLAFLLAISIYNQEQT
                     DGELLESDLIDLFRYVSNSFEQSDATFTQRANNAINDLVRQRLLNRFSSEFTEGLAIY
                     RVTPLGVGISNYYVRQREFSTLRLSIQLSIVADEIQRASVAAEEGIEQAQDERFWRNN
                     VFAPLKYSVAEIFDSIDLSQRIMDENQHQIRERIAELLSQNWHEAIMSCEQLLDETSG
                     NLRELQDTLNAAGDKLQAQLLRIQSCLMARNDLDFIDQLIVNLQNKLDRIISWGQQAI
                     DLWIGYDRHVHKFIRTAIDMDKNRVFGQRLRQSIQDYYNSPWLLYTAKADSLLDLRDD
                     EAMLNEAESVGELPSELEYESLSDVQEQIVSAMQAHLAPFRAEGKPIDLGVILREQLA
                     RFPESRHFDVARIIVDQAVKLGMASQDSQAVYPQWQPINEQGAEVQANVIDQYNK"
     gene            complement(17940..18827)
                     /gene="galU"
                     /locus_tag="PXE44_000947"
     CDS             complement(17940..18827)
                     /gene="galU"
                     /locus_tag="PXE44_000947"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL5588473.1"
                     /translation="MKVIIPVAGLGTRMLPATKAIPKEMLILADKPLIQYIVNECVAA
                     GFKEIVLVTHSSKNAIENHFDTSFELETMLEKRVKRQLLDDVRSIVPKDVTLIHVRQG
                     QAKGLGHAVLCGRTVVGDEPFAVVLPDVLLGEFTANQKTENLAAMVKRFQETGYSQIM
                     VAPVPMENVSSYGVADCHGVDIPLGGAAPIAKMVEKPSIEEAPSNLAVVGRYVFSSNI
                     WNLLEKTPVGVGDEIQLTDAIDMLIHQETVEAFHMTGRSFDCGYKVGYMKAFVEYSLN
                     HDKCGKEFKAFIKELAKSL"
     gene            complement(18963..19148)
                     /gene="csrA"
                     /locus_tag="PXE44_000948"
     CDS             complement(18963..19148)
                     /gene="csrA"
                     /locus_tag="PXE44_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDL5588474.1"
                     /translation="MLILTRKIGEVLLVGDDVEITVLSIRGNQVKLGVNAPKEISVHR
                     REIYERIKASVEEENAE"
BASE COUNT         5441 a         4408 c         3641 g         5660 t
ORIGIN      
        1 ctcaagccga tagactgttc caagtacttg aagtagaaac attgacaaaa tttctataaa
       61 aatcgaccgt ttattaactt ggagtattac atttttgaag aatcttcaaa atctctttca
      121 tatctttgcc catataatcc gctaaacttt ggtcaatcag tattcgtttt tcttcgtaac
      181 caatcaccgt taagcctgcg gttttggcgg cgagcattcc gtagtaggag tcttcaatcg
      241 ccactgcatt ttgtggctca acgcctaatt tttcgcaggt gtagcggtaa atggttgggt
      301 ctggcttgct gtgttcaaac tgttcaccgc tgacgattaa atcaaattcg tctttaatgc
      361 cgcaaaccgt gagaatattt tcaatatgcg aaagggcgga agaagaggca accgcaagct
      421 taatttgatt ctgcttggca aattgaataa tttgctgaat atcggctctg aaaatggttt
      481 taaaatccac tttgctaaac agatcttgga aaaaggcgta gtaacgtgca cgaatttcct
      541 caatcggcaa actggattcg cttagttttt tgataatcac cggaatttct tttaaacatt
      601 tgcctaccac ttccgattgt tgttctaaag taatcgggcg gctgtgttct ttgatgtctt
      661 caataaactg tttttgtaag gaaaattcca gatattcggt atccacaatc acgccgtcca
      721 tatcaaaaat gatagcttgg agcatatagc ctcttttgta aaattttaag aaaatctaac
      781 cacttattcc ctctctctgt tttaaattac tgaacgtaat ttaaaacggt ctctctccca
      841 caaggggaga gagtgagaaa ggctattaac ccattcggta gttcggtgct tctttggtaa
      901 ttgtcacatc gtggacgtgg ctttctttaa taccagcacc actaatgcgg acaaattctg
      961 ctttggtgcg gagttcttca atagtggcac agccggttaa tcccatacag gagcgtaagc
     1021 cgcccatttg ttggtggata atttctttca agtagccttt gtaagcgata cgcccttcaa
     1081 tcccttccgg tacgagcttg tcggcggcat tatcagattg gaaatagcga tctgacgagc
     1141 ctttactcat tgcacctaat gatcccattc cacggtagga tttgaatgct ctgccttgat
     1201 aaagctcaat ttcacccggg gcttcttctg tgccggcaaa catcgaaccg accattacgc
     1261 aacttgcacc ggcggcaata gcttttgaaa tatcgcctga ataacgaatt ccaccatcag
     1321 caatcacagg aatgcctcgt tctttaagcg cagctgccgc ttctgcgatt gccgtgattt
     1381 gtggcacgcc aacgcctgtt acaattctgg tggtacaaat tgaacccggg ccgattccta
     1441 ctttcacagc actggctcct gcatcagcta acgcaatcgc tccttctgca gtggcaatat
     1501 taccggcaac aatcggtaaa tcagggtatt ttgcacgggt ttcacgcaca cgttgtaata
     1561 caccttcaga atgcccgtgc gaagagtcga ttaatagcac atcgacaccc gcttttacta
     1621 aagcatcaat tcgttcttca ttgcccggac cggctcccac tgccgccccc acacgcaaac
     1681 gcccaaactc atctttacag gcattcggtt tgctttccgc tttttggtag tcttttaggg
     1741 taatcattcc ttttaactga aattcattat tcactaccag cactttctcc actcggtgtt
     1801 cgtgcattaa gtggaaaatt tcttcacggc ttgcgttttc ttttaccgtc actaaacggt
     1861 cttttggtgt cataaattca cgcactgttt tgcttaaatc cgtgacaaat cgggtatcac
     1921 gtccggtaat aattcccact aaattttggt tttcatcaat caccggatag cctgcaaaac
     1981 cgttcttttt caccaattca gccaattccg ctaatgtcat atcaggagaa atcgtcactg
     2041 gctcagaaac aataccactt tcaaattttt tcactttacg cacacggtct gcctggcgtt
     2101 caatcgacat atttttatgg ataaaaccaa tgccgccttc ttgagcaagg gagatcgcaa
     2161 gcttagtttc tgtaacggta tccattgcag cagaaagcat cggaatgttt aaacgaatgg
     2221 ttttagttaa ttgagtagaa agatcagcag tattaggaag cacagtagaa tgtgccggga
     2281 cgagaagaac atcatcaaaa gtgagggctt cttgtttaat tcgtagcatt gcaattattc
     2341 cttaaaaaat gattaaattg aaaatctgta ctggaaaatt gcgagcggat tatacagagg
     2401 aactaggttt ctgtaaatag ggaaacgttt acgctatctg aaaatatact ttcaagcggt
     2461 taaattttgt gattttttag caaacaataa ataaaaaacg accgcttgta agccaacaaa
     2521 aaagagcgta tcgctacgct cttttgctat ttaaaactat gcttcttttg tcgttgggaa
     2581 atatttttcc tcgtcaaatt cacgtaatgg cttgtgttct tgccctaaga aagtgtagat
     2641 aaccggcaag ataaacagag taaacaatgt cccgattgcc aagcctgcta cgattaccat
     2701 acccatactg aagcgggcga ttgcaccggc acccaccgca aacagaagcg gaattaagcc
     2761 tgcaatcatt gctgccgttg tcatcataat tgggcgtaaa cgaatcgtgg ctgcgtggaa
     2821 aatagcttca tagcgtgatt tgccgtgcag taactgctct tctttcgcca cttcacacat
     2881 caagataccg tgtttagtaa ttaagccgac aagggtaatt agacctactt gtgaatagat
     2941 atttaaggta taacctgccg tccaaatacc gttggcgaat gccatcatat ctttcgagcc
     3001 agtgcctgct gcaatcccca taaacaagcc tttcattgcg gttggaatgt tcattaccaa
     3061 caatgcacca ctcacagcta acggcacaga aaccaaaatt actaatgggt cacgccaaga
     3121 ttcaaattga attgcaagta caagatagat aatgataact gctagagcga aagtcattgc
     3181 catcgcatta ccttcttgaa cgaactggcg agattcacca cggaagtcat attgataacc
     3241 tgatggtaaa gtgcgatcta attctgcttt cgcccaattt accgcatcgc cgattgagcc
     3301 agatactacc ccaccaatgg tggccgagtt taattggctc atacgaggta aagaagatgg
     3361 tgatgttttt aattccatcg tcacaaatga acctaacggg atagattgcc ctgttgcagt
     3421 cgtgacatag taattcatta aatcttcagg atttaaacga tcattacgca ccgcttgcga
     3481 cacaatcggg taagcacgag agtcaatatc gacacgagta ataatcgcac cggataagaa
     3541 tgcccctaat gtgccactca cctgctgcat tgtcacgcca taagcaccca ttttttcacg
     3601 gtcgaatttc atattcatcg tgaccgtatc aaatttcaaa tctaaattcg caaagaagaa
     3661 ctgaccggaa gtttgggctt ttttcaagaa ttcagaggca acgattgcca atttttcata
     3721 gctatcagcg gtagtaatca ctaatgagaa tggtagacca ttttcaccgg ttgaaatttc
     3781 agggaatgca attgcattca cgtccatacc caccactttt ttaccgattg cagccacatc
     3841 attagaaatc gcagcacgtt cacggctacg atcattccaa tcttttaatg caataatcga
     3901 taatgcacta tttgcaccat taaagcctga aactgtcatt aaggaagata cttcaggtaa
     3961 tttagttact tcctgattaa attgggctaa cgctgcttcg gtataatcta aattggtgtt
     4021 tgaaggtcca ttagaaatac caaccacaaa gccacggtct tctgtcggtg caacttcact
     4081 ggaaagtgag ccaaataaca tcggtaaact ggcgaagatc agcaccgcaa agaataagac
     4141 gaaaccacga acagccataa taccgcttag catattggta tagcagctgg tcattttatc
     4201 tagtgatcta tgcaccacgc gggcaaatgc tgactcttcc ccttctttat gttcttttaa
     4261 tattcggcta gacatcatcg gcgaaagggt taatgccgca atccccgaaa taaataccgc
     4321 accggcaagc gttaaagcga actctttaaa tagtgtccct gttaccccgg tcattaatgc
     4381 cattggtgaa tatactgcac ccagagtaat agtcattgaa ataaccggta aagcaatttc
     4441 acgtgtaccg ataatcgctg catcaaatgg cgttttacct tctttaacgt gacgttctac
     4501 gttttcaagt acgacaatcg catcgtccac taccaagccg atcgctaata ccaacgccag
     4561 caaagtaagc aagttgatcg agaaaccaaa catttgcagg aagaataata ccccgattaa
     4621 ggaaatcgga atggtaatca ccggtacaat cattgctcgt aatgaaccta agaacaggat
     4681 aattactacc aatacaatga cggttgcctc accaatagtt ttaattactt ctttgataga
     4741 actatcaatc gcaatggttt tatcgtaaac aatatctcct tgaatggtgc ttggtagatt
     4801 ttttaatacc tgctcaaaca tcggataaat atctttcgct acggttaaag agtttgccgt
     4861 tgcggctacc gaaacagcaa gcacaactgc atctttaccg ccaaccgttg ctcttgctgc
     4921 atcttggtat ttatccagct caacagtcgc aatatctttt aatttgatgg tttttccatc
     4981 tttggtggtt gaaaccgtaa cctcttctaa ctctgtcaca gaaggcgtgg tagaaagtac
     5041 tttgtttcta taaaccgtat agtaaccgtt agaattaccc gcagcggttt gcaagttatt
     5101 ggcacttaat gccgataata cagccgcccc tgataggtta ttgctcgcca ttttatccgg
     5161 atcaagccag atgcgtaagc caaaaacaga gccatacaca tcaacgctag aaacaccatt
     5221 taccgtaaag aactgtggtt ttactacacg gttaatataa tcggtaactt gtgatgtatt
     5281 aagttcatca gagacaaatc ggatatacat taacgcatca tttgaaccgg ttgagactct
     5341 aattgacgga tcatcaatac cgctcggtaa atttgagcgt accgcattca cttttgccga
     5401 aatatcggaa agtgcggcat tcggatcggt atttaacttc atttttgccg ttaccgtagt
     5461 ggtacttgga gagcttgatg aagtcatata gtcaatatta tccgcttgag caacagcctc
     5521 ttctaactgt gaggtaacca gtgtctgcat taaacgtgca tctgcacctg aatagttaac
     5581 actcacggtt acaatcgaga ccgtcatctc cggatattca cgcacctgca atttttcgat
     5641 tgcttgcaag cctaggatcg taatcaatag gctgatacaa actgccaaaa caggacgttt
     5701 aataaagatg tcagtaaatt tcacttaact cactccacta cagtttactg tttttcgccg
     5761 gtgtggtaat gcctacggct tctgtatcag aaactttaac caacgcatta ttacttaaac
     5821 gttgtaaacc gcctgttaca atcagatcac ctgctttcac gcctttggat aattgtgaat
     5881 agtttccttt gcggtcagcc gttttcactt ccatctgttt tgcacggtac attttgttta
     5941 catctaaact tgggttttgt accgccattt tgttcaccat ttctttatct tcatcagata
     6001 atggttgcag cacatatacc gtttcaccat acatagtgta agcgatagaa atttgtggta
     6061 cgacgatttg tccggtttct gttggtaaag caacatttaa gcgggcaaac atacctgaaa
     6121 gcaatttttc ctgcccttct tcaatcaccg cttccacatt aattaagccg gttaaacgat
     6181 ccacagccgg atcaatagcc acaattttag ctgggaaggt ttgtgccggt aacgcatcaa
     6241 ttacggctgt cactttttgc ccaatcgaga tttgctctaa attagtttgc ggcaaggtaa
     6301 aacgtacttt cattgaagat tgatcttcca cacgcacaat ttccgtaccc attgtaatgt
     6361 attgcccaac attcacattc acgatacccg ctaaaccact aaacggagca taaatttgac
     6421 ggcgtttaat cgtagcacgc aatgcctcga tattggctaa taattggttg taggtagatt
     6481 gagcattatc aaactcggct ttagaagcac tgtttgaggc aatcagatta cggtaacgct
     6541 ctaaatttgc tttggcattc ggtaattgcg cttcagaggc tctcaagtta gcaatttcaa
     6601 cactgctgtc aaattctact aacagatcac cttttttcac acgctgtcct gaagtgacca
     6661 aaacacggct aaccacgcct gaagtttcag cacttaacat tgccccttga tttgggcgaa
     6721 tataaccgac cgcagaaatg gttggtgtcc attcttgggt cgtcacttgc atagcagtaa
     6781 cttcgctcac cgcctccggc atatttgccg ctgcttcggc ttttttcatt gccccgattt
     6841 tttgtaaccc tacgattgca gcaaacacaa tcaatacgat taacaatgtg agtacaatta
     6901 aaaactttct gcctttaccc agcttctgtg tttccgttgt catacattct ccataaaact
     6961 aataaaaaga aacttaaaaa gtgattgctt tccaagtgcg taaaataatc tcgtctaaat
     7021 atccatcaaa ctgattttct tgattcagat tttgcagata ttggatatcc accaatgtgc
     7081 cgatgcttaa tccataaagc aactcattag atagatcagc aataatcctt tgctttttcc
     7141 cttctaaaat taatttattc caaaaggaag tttcatcagc tatggtttcc cgaatcaatt
     7201 catcaatccc tatcaccgct tgaaactgtt ttaccaaatc taaacgctcg attaaaaact
     7261 gccatttttt attgaccaac gctttatatt gctcaaataa cgccatttca ggcttaaact
     7321 caaaatgcat ataaccgtta taacattcga gtaattgggc agccaatgag tgcaataaat
     7381 catctttggt tttaaagtaa agatataatg taccggaagc gatccctgct tcttttgcaa
     7441 tactacgcat tgaaaggttt tgcaaacctt cttgcacaat cagcgactcg gttgccgata
     7501 aaatacggtt taccatctct tcttttggct cgcttcgcct agacatttta tctccttacc
     7561 tataatgaac agccgttcat tatagctttt tttatgaaaa aagaaagcag aaaacaccta
     7621 tcaaattaaa ataaaatagt tgcaaaattc ctcatttatt gaacaagcgg tcatttttgg
     7681 ctaaaacttt gcaaaaatca ctcaaacaat aacgcttaac gctactttat tattattttt
     7741 gtgctactat ttgtacctaa ttataggatc atctttattt caatatggca acgaaatcta
     7801 aataccacac caaacaattt gagcagctat tggctgacct catcgccacc atagaaaagc
     7861 ataaagcacc ggtggattta tcgttaatgg cattaggcaa tatgataact aacattttaa
     7921 ttgaaaatat tcaaagcccg gcacaaagag aagctctcgc tgatgccttt gccgatgcat
     7981 taaaacaatc actaaaaacc aaataagatg atgtttgcac gcctacgcag tttattgccg
     8041 accaattctc gccaatatcg agaagaaacc tcacagaaaa tcacgtgggg gcattggttt
     8101 gcactattta atattgtgtt ggcactactc atttcctctc gttacgcatt taatgcagac
     8161 tggccgaaca cgcttgtagg caaactttat ttctttgtca gcctgttcgg gcatttcagc
     8221 tttgtggtct ttgcaggatt tctattaatt ctgtttccgc tcagttttat tgttaagaat
     8281 catcgcacct atcgtgggct ttccgtgatt ttagccacca tcgggcaaac cgtgttattg
     8341 gtggatactg aagtcttcaa gcaattttat ctgcatttat caccgcttgt atgggatctg
     8401 ttagttaatc cggaagaagg ggaactgacc cgtaaatggc aactgctgtt tgtaccaatg
     8461 ccactccttt tgctggcaga aatgctctac tctcgctggt gctggcagaa actgcgtagt
     8521 tttagccgcc aaaaatgggg caaatatgtc gctctctttt tcgtgatcag ctttaccgct
     8581 actcatttaa tctatgcgtg ggcagatatg aatgcctacc gcccgattac cgctcaacga
     8641 gcaaactacc cactttctta cccgatgacg gctcgtaatt tcttagaaaa acacggtttt
     8701 attgaccgag tagaatttaa tcaagaaatt gaagaaagtg gcagactaga tactttctat
     8761 ctgaattatc caaaatctcc cttgattgcg accaaaacgc cctcaactaa tattttactg
     8821 attaatttat caggtttatc taaacagata ataaatgccg aaacaatgcc taagctgaca
     8881 gaaatcagcc aacaaagcca ccgctttatg cagcattaca gtagtggtga ttcagcctct
     8941 gccggtgcat taggtctatt ttacagttta agcggtaaat atttggattc cgttttagcc
     9001 gataaaaccg tgtcaccgct tatcgaaatc gctagaaaat cggactatca attagggctg
     9061 ttttcacata atggttttgc cgacccgatt tatcatcagg cgatcttctc tggtatttta
     9121 ttgcctacgg cgcacagtaa tgtaggggcg attgggcaat ggaaaaactg gatcagctct
     9181 cgctcattgg aaaatccgtt ctttagctat ttagacttaa gaatgccctt cgcccacacc
     9241 gctcaagagc aggcaaaatt attagattgg caattcgctg aaatctggca acatttgacc
     9301 caaaatgcac tattggcgaa tacgctggta atcattaccg ccgatattag taatgaagtt
     9361 ggagcagaaa atagcttcga taaacaacac attcaagtgc cgatgatgtt ctactggcaa
     9421 ggcgaaagca aagagtacca tttcctttca agccatttag atattatgcc aaccttactg
     9481 agccaattct tcggcgtgca aaatccgctg aatgattatg cacaaggtat ggatttaagc
     9541 acccagaacc aacgtttatg ggtatccagt tctaatcaca aatggaatgt ggcgattatg
     9601 ccgaatggcg atcaatatca aattgatcgt aaaggcaact ttaagcactt taacagcaat
     9661 ggcgaacacg agaaagatgc tcgaccacca ttagcgttgt tcttacaaat gattcaacaa
     9721 agcaatcaat ttattgaaaa gtaatgttca acaagcggtc atttttgcag gaaaatttgc
     9781 aaaatttcag caaaatatga ccgcttgtca ctatctgagc tgtctatatt tcaagctaac
     9841 aaatacaaac caaaccattg ctacaaaagc aatactccca ccggcaaatc cgatattggc
     9901 taatcccagt tgctgaatag cgatgctccc cagtaatgct ccaccgccaa tgcctaaatt
     9961 ataagtgccg gaaaaaattg cagttgcgac atcagtcgca tcaggggcaa gttcaagcac
    10021 cttcatttgc aacgcaatgc tcattccggt aatgccaatt ccccagacaa aagcgaccat
    10081 aaacattgcc actgttgagt cactcaaaaa cagtaaacaa agctggctgc acataataat
    10141 ggcgatcccg cttaagataa atttattcgg ggctttgcca tataaccgac caaataaaaa
    10201 acttgccacc acacctgcca agccaaaaac cagcaaaata aaggtggtgt tttccacact
    10261 caccgaactg attttaatca taaacggctc aatatagctg taagcggtaa aatgtcctga
    10321 aacgatcaat gccaccagca aataaatgcc gattaacatc gggcgtttaa ataacaaagg
    10381 caaacttttt atcgagcctg catttttgct tggtaaataa ggcagcaacc gccaaatcat
    10441 cattagcaca atcaaagcga taatcccaat aatgccaaag gttgcccgcc agcctaaggc
    10501 ttgcccgatc attcgcccat aaataacatc agcaacagcc gttttctttc catattcgcc
    10561 gtgagtaaca tcaacggtaa cgataataaa aataccaccc acgcataaac cgtcatcata
    10621 atgcctgccg atgaaggctc catttggaaa ctgcttgcaa tgtctgaaag caatgcaata
    10681 ggaataaatt cggtggtgtt tactataaaa gccgcaaagg caagactaaa tactctaagg
    10741 aacgataatc tccgttgagc ttttcgagca agtttcatat taattaaatc cgagaaaaac
    10801 acatttattt tcaaaatttt aaaaataaat gtaaaaacaa aatcaaattt ccaaatttct
    10861 gaaaataaaa agattgccaa tgtttccatc aacaatcttt ttatacatat ttagattaaa
    10921 gtgctaagtt ttatttagca aagcctttta aaccaacaac gtgaacatac tcttggttat
    10981 tggtgatttt acgcaccaat ttataagttg tgcctcgttc agggctaata ttttcagggg
    11041 ctgcaattaa taactgcata tccaaacgtt cgcagagttc aaatagcgta ttaattgagg
    11101 tagcatctaa tcgagccgct tcatctaaga acaataagcg agccggtaga atatctttgg
    11161 cacgcatacg gcgagcttct tcttcccagc tttgaactac cattaagaga atcgacatac
    11221 ctgtaccgat cgcctcaccg gtcgataatg cactactttc tgcacgcatc cagccgtctg
    11281 caccacggaa ggtttccact tctaaatcta agtagttacg gtaatccaat aactcttcac
    11341 caatggtctg tggtgtacgt tgccccatct caatatgagg gttcacccgt ttatacaaca
    11401 tcgctaaggc ttcggagaag ctcagttttt ggctgtcgaa taaatctttg tgttcctcac
    11461 gattattgga taacgcattg agtaaaatag cgtgcgtatc tcggatattc accaccaaac
    11521 gcaccccttt tacctgaccg aatgcaatat tttgtaaacc ttggttcagt tgcaaaatgc
    11581 gttgttgttc acgctgaatg gttttacgta aaatatttgc cacactttcc gcactgatcg
    11641 ccagtttttt ctcacgaccg gttaattcat tggttaagcg tgaaagctcg atttccatct
    11701 gctcaatcgc atcaatcgga tcatcggttt tgataatgtc ttggcgaatc cgctcacgca
    11761 aatgctgata aaccgcaata aagaacgcca ctttgttttc cggcttgcga ctatcttcag
    11821 aagcccgcaa gctatcacgc aggtattcat tatccgccac ggcggtgcgt aatgccccga
    11881 gagctttatc cgaaatcgaa cgtaattctt cagccgattg ataagccaat tcacggcgat
    11941 ttaaacgttt ttccacatcg ctgttacgag ataaacgttg cacaatacac cagctggctt
    12001 ttgcctgcac caccaattca cgttgtgtat gataatcacg ttcggcttta cgtaaggtgc
    12061 ggttaaggtt atccatttcc gcctcgatca ccgcaatttg tttatctaaa taacctttgc
    12121 gggaacgttg ctggcttaaa cgttgttgca attcatcacg gcggaatctg gctcgctctt
    12181 ccgctccgct atcaccacgc acgcccaaat cgtcaatttc acgaattaac tcttgtagca
    12241 tttggttttt cgcctcataa gaactgcgta agccggtcaa tacttggtta tattgtgaaa
    12301 gttgagcttg agcttgacgc agctgatcac gagcctgttc acgctcacgt tgtgcatttt
    12361 ccaaacgttg gcgtagctgt tcggttaatg cagagccttc tgtacccacc gtttcttgat
    12421 agctaaaatg gaggcggcga ttcattacat cagccaagct gaacactttt tgctgtaaca
    12481 ctttttgctc ggcaactgaa cggcggtaat cggcttccaa ctgttcaaat aaagccggat
    12541 cactttttaa tgcggctgca atcggctcaa gttggcttaa tgttgctcca aattggcgga
    12601 taaacatttc atcttcttct gccaattcta attgctcacg acattcctcc gctcgtgctt
    12661 ccagcgtttc atcggctaat aaattcgcaa gcggtagaat tttattcaac atttgcaact
    12721 gggcttttac gctttctaat tggtgacgta attgctgttc gttaccggct gattgtgcca
    12781 attcacgttc aatttctgcc cgttctgccg caatctcttg cattaacatt tcagggtttt
    12841 cttggaacgc taaatttaag tgcgtgccga taaattggct taagtgttga tgtaaacgct
    12901 ggcatttctg cacatcaaag gcacgttcag cgtgtttttc tgcggtttca tcacgctctg
    12961 cttttaaagt cgctaaatgt ttttcacgag cagcacgtcc gaataacggc acttcaggga
    13021 atttagaata acgccattgg cgatcggaga ctttcaccac cacgccttcg cctaattcct
    13081 cggcactaaa taccgcatcg tcaaaggcgg acggatcacc ttcaattaaa taaagatcac
    13141 ttgggcaatc gtctaatttc tctagctgtg ttttaaccgc ctctaaatca cgcactacaa
    13201 tagcgtggcg agcctcgccg tataaggcgg agaaataagg cgcatcttca attgatacat
    13261 catcatataa ctcagagagt aacactccac caaaacgttc tgccaactgg tttaagcggg
    13321 cgtcttccga gccgtccggc tggcttaagc gattaatttg ctcatcaagc atttgttctt
    13381 tacgagccaa ttcatcacga gcaagggtgg cttcacgctc tttgctcagc atattttgca
    13441 taaactgcat cactgattgg ctggcttcaa acttctcgcc acattgctct tctaaacgag
    13501 tcagagccga ttgtgccgta tgccaagccg gtgcattctt cgctaattgc tgatattgtt
    13561 gattaagctg ttcacgttgt tggcgttggg ttgaacgttg ttctacaaag tccgaaagct
    13621 ctgcctctaa atcttctagg cgagcttgct gctcttcaaa ataggcgtct aattcttccg
    13681 ctgaattgag ttgttgttgc gatttttggt taaattccgc cactaaacgc tgggcatttt
    13741 gctgttgttg caaacgctgt tctaaatcgt tgagtttttg acgtaaactc accgcttgct
    13801 gagcttgcat tttttgctca gggaaggcag aaagtaacgc acgagcctct tcccacgctt
    13861 gcaagcggtc aatttcaccg gaaattttgc aaaccaattc aaacgctttt tcaaattggg
    13921 ttttcgccat atccgacacg cttaaacgtt gttccagctc aaatacttga tcggtaagat
    13981 cttcggcttg agccacaaat tctgcgtggt aatcttcaat attatttaaa tctaaattca
    14041 ctaagccggt tagctgtttg gctttttcta acgcattcac cgcttgttgg tattgcaatg
    14101 cacgagtttg ctgtgcatct aaggcttgtt ggtaatccgc catttgagaa cgcaattctt
    14161 caacgtgatc atctgcctca tccgcacgag cctgagcatt ttcggcaatt tccgcaattt
    14221 cttccaatgc ctcttgctgt tcttccagct taatattcag ctcttcaact tcgtcttggt
    14281 agcgttcgat tttctcttgg tggcgtaacg cattcatcac taaatttaga tgatcgttcg
    14341 cactattgta atccgcctct aacgccagct ctgcttcggc aatatccgca ctttcacggc
    14401 taaattccac aaagcgttgc tgttcgagca aaattttgga ttttgccgca taccactctt
    14461 tgcgttgctc taaggcttgt tgtacattgc cacggcgttc attcgcatta cgcatataat
    14521 ccgccgacac ataattggtc gattcggtaa ttaagcgttt gaacatatcc cgatccgatt
    14581 gcgtgacctt aatcgcctct aaggtcatac ggttttcacg cagagcggat tccatatctt
    14641 ggaacgcttg gcgaacgccc gtattttccg gcaacaagta atcacgcaag gatttggtaa
    14701 tcacacttga aataccacca taaagcgagg cttcgattaa tttgtagaat ttgctacggt
    14761 cggctgccga acgaaggcgt tttgggatta cgcccaaatc gaacataaaa ctgtggtaat
    14821 cagtaatcga atggtactgt ttgaattgga cttcgctgtt ctcaaattga tctttgagtt
    14881 catttaacgc cagcactcga gccttattcc ccacttgttc ggtcagtagg ctgataatgc
    14941 tttgctcttg aggaatattc tggagcgaga acgaacgaat atcgactttt ttatcccgtc
    15001 cgacaatttg ttgcaaacgc acgccggtaa taatccgttg cccacgggaa ttgaatgact
    15061 ctaacaccgc atagcaaaca cccgctttta acttaccgta taagccttta tcacgagaac
    15121 cggcggttga gcctgcttca gtcgtattac ggaaattaag caaggttaaa tctgggatca
    15181 aggcggtcac aaaccctgcc attgtggtgg acttacctgc cccgttaccg cctgaaagcg
    15241 tggtcactaa gtcatctaaa tcaaaggttc gagcaaaaaa accgttccag ttaatcagcg
    15301 ttaaagagag aaatttaccg cgttgtacat cattcgggcg tgcaagcggt gcaatttgag
    15361 gattattttg caattctgtg gtatcaatca ttatgttttc tcactaattg tcctagcacg
    15421 ctaaagcgta ctaggttagg tataggtgag tagctccgct actctaatcg caacctcagc
    15481 cttggagggc tgctttatgc ttaacctagt tcggctttac cgtgtctagg gcagactcat
    15541 taaatgctga atctattcga atgaaatcac tcattttctc cgtcaaaggg ggcttcatct
    15601 gcaatatttt catctatttc aaccgcttgt tcgtttaaca acgcaggggt tgtcgcttca
    15661 ccatcacgaa ttaagcgaag ctgtgcctct cttgggtcat caccggtacg cacatcagcc
    15721 ccaaaacgga agaccgattc tgaaatcaca aattttttgc tgttttgctc gcctactcgg
    15781 gtaataatgc cgatacgggc taatctgcgt aacgctccgc cggctttttc tgccagcttg
    15841 gcacggtcta aatcggaacc ggtcgaacgt gggtttaccg cttttaataa tttgctttca
    15901 tcggcaagac tgagtaattc ttcatacaca tcatcttggc tgaaaatccc ctgctgagcc
    15961 aaacgttccg gacttaaata gaggtagcac aacaccttac ccactaacat ttccatttct
    16021 gacattgccg aacgggcaat tagtgtcgag gctttcgggc gtaggtagaa aaagccttcc
    16081 ggtgctcgaa tcaactcaac gtgataacgg cggtaaaaac tctctagttc cgcttgaaaa
    16141 tccatcaaaa agacgtgttg gtcaagctgc tcggtattga tatggcgacc ggctcgtaat
    16201 tggctgtcta gctccggaaa aatcgggttg gcaatcgcaa gtgccagttt cggggaaatg
    16261 aaatcttgga tattctctgt cattgtcatt ctcttcaatt ttgcaaaatt ctgggtaaaa
    16321 acgaccgctt gttatttatt gtattggtcg ataacattcg cctgcacttc cgccccttgc
    16381 tcgttaatcg gctgccattg tgggtaaacg gcttggctat cttggcttgc catacctaac
    16441 ttaacagctt gatcgacaat aatacgagcc acgtcaaaat ggcgggattc cgggaatcgg
    16501 gcaagctgtt cacgcaagat aacgcctaaa tctatcggtt taccttcagc acggaacggc
    16561 gctaaatgag cttgcattgc cgaaacaatt tgctcttgca catctgaaag cgactcatac
    16621 tctaactcac ttggcaactc gcccaccgat tctgcctcat ttaacattgc ttcatcatca
    16681 cgcaagtcca gtaacgaatc ggcttttgca gtataaagca accaagggct gttgtaataa
    16741 tcttgaatcg attggcgtaa ccgttgcccg aatacacggt ttttgtccat atcaatcgct
    16801 gtacgaataa atttatgcac atggcggtca tagccgatcc aaagatcgat agcttgttgt
    16861 ccccagctga taatacggtc taacttattt tgtagattca caatcagctg atcgataaaa
    16921 tccaaatcat tacgagccat caggcaactt tgaatccgca ataattgagc ttgcagttta
    16981 tcaccagcgg catttaaggt atcttgcaac tcacgcaaat tccccgaggt ttcgtctaac
    17041 aattgctcac aactcataat tgcttcgtgc cagttttggc taagcagttc ggctattcgt
    17101 tcacggattt ggtgctgatt ttcgtccata atccgttgtg aaagatcgat gctgtcaaag
    17161 atttccgcca ccgaatattt caatggggca aacacattat ttcgccaaaa acgttcatct
    17221 tgtgcctgct ctattccctc ttccgctgct actgaagctc gctgaatttc atctgccaca
    17281 atcgaaagtt gaatcgataa acgcagggta gaaaattccc gctggcgaac gtaataattt
    17341 gaaattccca cccctaacgg cgtaacacga taaatcgcca aaccttccgt aaattcgcta
    17401 ctaaaacggt ttaacaaccg ttgtctgact aaatcattaa tcgcattatt ggcacgctgg
    17461 gtaaaagtgg catcagattg ctcaaacgaa tttgaaacat aacggaaaag atcaataaga
    17521 tcactttcta acaattcacc atctgtctgt tcttggttat aaatagagat agcaagtaga
    17581 aacgctaagc ggtcggaaga aagcgaaagt gaaaattcac gatctttcgt ccaagagatg
    17641 agttctggaa tagtctgtgc caattcgttc tgcatacggg cttaatcttt aatttctata
    17701 caaaaatggt atttattgta actgaatgta atcaaaatgg gaatggataa aacgcagaca
    17761 agagaaatga acaatataag cggtcaaata tgggtgattt tttgcaaaaa gtattttaaa
    17821 ggtaaatgaa tccgaaaatt tattatcact acgctaattt atgataccat aaatagaaaa
    17881 gactggatcc aatagaatat cagacacagc cccttagatc atagtctaaa ctctaaggct
    17941 tagagtgatt tagctaactc tttaataaat gctttaaatt cttttccgca tttgtcatga
    18001 tttaaactat attctacaaa tgctttcata tagccgactt tataaccaca atcaaatgag
    18061 cgccctgtca tatgaaaagc ttcaaccgtt tcttggtgaa tcaacatatc aattgcatcc
    18121 gtcagctgga tttcatcacc tacgcctact ggcgtttttt ctaataaatt ccaaatattt
    18181 gaggaaaata cataacgtcc tactaccgct aaattagaag gcgcctcttc aatacttggt
    18241 ttttcaacca ttttcgcaat aggcgctgcc cccccgagag ggatatctac accatggcag
    18301 tcagcaaccc catagctact tacattttcc attggaaccg gcgcaaccat aatttggcta
    18361 tatccggttt cttggaaacg cttcaccatc gcagctaaat tttccgtttt ttggtttgct
    18421 gtaaattcac caagtaatac atctggtaat acaaccgcaa acggttcatc gccaaccact
    18481 gttcgtccac atagaaccgc atgacctaaa cctttggctt gcccttggcg aacgtgaatt
    18541 aaggtaacat cttttggtac aatagaacgt acatcatcta ataattgacg ttttactcgt
    18601 ttctccagca tcgtttccaa ttcaaaagag gtatcaaaat ggttttcgat agcattcttt
    18661 gaagagtgag taaccaatac aatttcttta aaacctgcag ccacacattc atttacaatg
    18721 tattgaataa gaggtttatc tgcaaggata agcatttctt ttggaatagc tttggttgcg
    18781 ggtaacatgc gcgttcccaa tccagcaaca ggaataatga ctttcatttg gaatcctttg
    18841 atgacttaac atattagata taactgaaat aaaccgctta gacataacag gcggtcaatt
    18901 ttcagaaaat tttgcattgc ctgcttcgct gttatcatct agcagcttta cagttaaaat
    18961 gcttattccg cattttcctc ttctactgag gcttttattc tttcataaat ctctcggcgg
    19021 tgtacagaga tttcttttgg tgcattcaca cctaatttaa cttgatttcc tcggatgctg
    19081 agtaccgtaa tctcaacatc atctcctacg agtaaaactt ccccaatctt acgagttaga
    19141 ataagcataa
//
LOCUS       DANLZC010000035        18877 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.035, whole genome shotgun sequence.
ACCESSION   DANLZC010000035 DANLZC010000000
VERSION     DANLZC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18877
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.035"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            45..995
                     /gene="tal"
                     /locus_tag="PXE44_000949"
     CDS             45..995
                     /gene="tal"
                     /locus_tag="PXE44_000949"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDL5588475.1"
                     /translation="MSQLDTLSKMTVIVADTGDIEAIKLYKPKDATTNPSLILSASAL
                     PQYAPLIEEAINYAKAQSSDKDQQLVDAEDKLAVNIGLEILKLVPGRISTEVDARFSY
                     DTEKTIAKARKLIGLYNDAGISNDRILIKIASTWQGIKAAEQLEKEGINCNLTLLFSQ
                     AQARACAEAGVYLISPFVGRILDWYKAHEGKEYAPAEDPGVISVTNIYNYYKQHGYDT
                     IVMGASFRNVGEIIELAGCDRLTISPPLLKELQESDEKLVCKLDYKGEIKPRPEKMTE
                     AEFYWEHNQEPMAVEKLAEGIRKFAIDQEKLEAMLAEKLQ"
     gene            complement(1057..1330)
                     /locus_tag="PXE44_000950"
                     /pseudo
     CDS             complement(1057..1330)
                     /locus_tag="PXE44_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
     gene            complement(1361..1825)
                     /locus_tag="PXE44_000951"
     CDS             complement(1361..1825)
                     /locus_tag="PXE44_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL5588476.1"
                     /translation="MKKLALAVALMSSFALVGCANTDVFSGSVYSGEQAKEARSIAYG
                     TIVSVRDVKIQARSEGVIGTVGGGVLGGVAGNAIGGGTGQAIATAVGAVAGAVIGNQV
                     EQKASQVSSLEMVIRKDDGKEIVVVQKKEDGFVPGKRVRIVGSNSDLNVSLL"
     gene            complement(1846..2445)
                     /locus_tag="PXE44_000952"
     CDS             complement(1846..2445)
                     /locus_tag="PXE44_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5588477.1"
                     /translation="MDKIIAENAKFFEKMTACFGDLSQFEQKIRQIAEIAGIDLAAYE
                     IDHLAVRMNQTETAEQWKILLLDGATLLKESEVNGRPIGLFALIQAVEFCGQFVKIVE
                     LPFPKNKVYLEEGWEHIELVFPMLDNESSEQWVSRALSHFRLADNSEIKLKISQPQVE
                     GERLPNPSIAISLRNVTFCNPYCLKLHPYDIKSVIMSEN"
     gene            2479..4206
                     /gene="argS"
                     /locus_tag="PXE44_000953"
     CDS             2479..4206
                     /gene="argS"
                     /locus_tag="PXE44_000953"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL5588478.1"
                     /translation="MNIQQILSDKIKQAMVASGADSNVEPLVRQSGKPEFGDYQANGA
                     MGAAKKLGMNPREFAQKILENADLNGIADKLEIAGPGFINIFLNPEWLAENTNSSLQA
                     VNFGIKTANPQTIVIDYSSPNVAKEMHVGHLRSTIIGDAVVRTLAFLGNNVIRANHVG
                     DWGTQFGMLIAYLEKMENEHASEMELSDLEAFYRAAKEHYDSDEAFAEKARNYVVKLQ
                     SGDKYCLTMWKKLVDITMHHNQENYNRLNVTLTEKDVMGESLYNPMLPEIVADLKAKG
                     LAVEDDGALVVFLDEFKNKDGDPMGVIVQKKDGGFLYTTTDIAAAKYRYETLKADRAL
                     VFSDSRQAQHMQQAWLITRKAGYVPDSFSLEHPFFGMMLGKDGKPFKTRSGGTVKLKD
                     LLDEAVERADKLIAERSTELTAEEKAAVVEAVAIGSVKYSDLSKNRTTDYVFDWDNML
                     TFEGNTAPYMQYAYTRIRSIFARANIDENALSGSIQLNEPKERALAVKLLQFEEALNG
                     VAKEGMPHILCQYLYELAGSFSSFYEACPILNAEETTKNSRLKLAALTAKTLKQGLDL
                     LGIKTVEKM"
     gene            4396..5562
                     /gene="cgtA"
                     /locus_tag="PXE44_000954"
     CDS             4396..5562
                     /gene="cgtA"
                     /locus_tag="PXE44_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDL5588479.1"
                     /translation="MKFIDEALIRVEAGDGGNGCVSFRREKFIPKGGPDGGDGGDGGD
                     VYLIADENLNTLIDYRFEKRYAAGRGENGRSAGCTGHRGQDITLRVPVGTRAIDNDTQ
                     EIIGDLTRHGMKMLVAKGGYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLKLELLLLA
                     DVGMLGLPNAGKSTFIRSVSAAKPKVADYPFTTLVPSLGVARVGADRSFVVADIPGLI
                     EGAADGAGLGIRFLKHLERCRILIHLVDIMPIDESDPAHNISVIESELYQYSEKLAEK
                     PIWLVFNKIDTIGEEEAAERAKDIAEQIGWEGDYYLISAATGQNVQALTRDIMDFIEA
                     NPREVEEENKAPEEVKFKWEDYHNEAMQNAVEEDWDDDWSEEDDEGIEFIYERH"
     gene            5647..6264
                     /locus_tag="PXE44_000955"
     CDS             5647..6264
                     /locus_tag="PXE44_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL5588480.1"
                     /translation="MLSSTSCDLFNIPFFQFAQMKKYCPEEIPRIKAEYKTQWEIWKK
                     LHQEVSRQLGQPFAPPHIEKWCNGWQVRAHFFAYYKYEFNRNSAAILSVILNRRRLQV
                     SLDWHCYRADRSQINVHQYNQWWENLDREKFADFEIWHGSESEYDDFRTVSQMSEQAF
                     TLTNEEDFWCIGKNVEKDDLDNIDVVEFIYQTIRALLPLYEKCHQ"
     gene            complement(6311..8269)
                     /locus_tag="PXE44_000956"
     CDS             complement(6311..8269)
                     /locus_tag="PXE44_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDL5588481.1"
                     /translation="MIAELGNYTLALALAISILLAVLPLWGAEKQNSTLIALARPMTW
                     AMFLALTVSFGSLFYLFAVNDFTVQYVVNNSNSRLPLQYRLSAVWGAHEGSLLLWVWL
                     LSLWSVGVATFTRKMPEDAVARVLGLMGVIGIGFLMFVIFTSNPFDRTFPNFPIDGKE
                     LNPMLQDVGLIFHPPLLYMGYVGFSVAFAFAIASLMTGRLDTAWARWSRPWTMAAWVF
                     LTLGIVLGSWWAYYELGWGGWWFWDPVENASLMPWLAGTALIHSLAVTEKRGTFKAWT
                     VLLAILAFSLCLVGTFLVRSGILVSVHAFASDPTRGLYILAYLVLVVGGSLLLYAFKG
                     SEIKSLDNYERYSRETMLLINNILLMAFLSVVFLGTLLPLIHKQIGLGTISIGAPFFD
                     QMFLILMVPFSFVLGIGPLVKWRRDQVSAIRKPVIIATILMALLGFGLPYLFRNTITV
                     SVVLGTMMSVFIVLLSLYELHQRATHRHSFLSGIFKLSRSHWGMVLAHLGVAMTVFGI
                     AFSQNFSIERDVRMNVGDKAEILDYQFEFKGIKITDGANYQGGTAELEITRNGKYEAT
                     LNAEKRFYTVSRMGMTEAAIDWGFTRDLYAALGEKLEDNSWAVRLYYKPFIRWIWIGG
                     LFMALGGLLCMLDKRYRLRIENKKEVIA"
     gene            complement(8266..8823)
                     /gene="ccmE"
                     /locus_tag="PXE44_000957"
     CDS             complement(8266..8823)
                     /gene="ccmE"
                     /locus_tag="PXE44_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDL5588482.1"
                     /translation="MNPRRKSRLKVVVSILLGLSVAAGLTLYALSQNIDLFYTPSEIV
                     NGKNDDPKQKPEVGQRIRVGGMVVEDTVKRDDKSLKVVFDLNDIGPSITVEYEGILPD
                     LFREGQGIVAQGVLIEPTRLKATEVLAKHDENYMPPELGDKLKEQHKKSGVSEQDLKG
                     ESETDRKMKAEAEAEKANNVQGESK"
     gene            complement(9009..9206)
                     /gene="ccmD"
                     /locus_tag="PXE44_000958"
     CDS             complement(9009..9206)
                     /gene="ccmD"
                     /locus_tag="PXE44_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDL5588483.1"
                     /translation="MTFQFESISDFFAMGGYGFYVWLSYGVSFIAMIGLIWLSRREQK
                     AILAQAKKEIVREEKLKQTSQ"
     gene            complement(9221..9958)
                     /locus_tag="PXE44_000959"
     CDS             complement(9221..9958)
                     /locus_tag="PXE44_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HDL5588484.1"
                     /translation="MWKWLHPYAKSETQYHLLGKIQPFLGWLSLIMLAAAFVWGLAFA
                     PKDYQQGDSYRIIFIHVPAAIWSMGVYGSMAVAGLVALVWQIRQANLAMISMAPIGLV
                     FAFISLATGAIWGKPMWGTWWVWDARLTSALVLFFLYIGVMALYSAFQDRQTGAKAAA
                     VLSVVGVINLPIIHFSVEWWNTLHQGATITKFDKPSMAVEMLIPLLLAIFGSLIFTAW
                     FSIWRYRIALLKDERNRPWVKQLAKSV"
     gene            complement(10158..10820)
                     /gene="ccmB"
                     /locus_tag="PXE44_000960"
     CDS             complement(10158..10820)
                     /gene="ccmB"
                     /locus_tag="PXE44_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDL5588485.1"
                     /translation="MILLTIIQRELTIAFRKPSEILNPLWFFLMVITVFPILMGPNPE
                     LLGKIAGGIAWVAALLSALLSFERLFRDDYLDGSLEQLMLLPLALPQVALAKVIAHWL
                     LTGLPLILLSPIAAILLSLETHVWWALVLTLLLGTPILSCLGAIGVALTVGLRKGGTL
                     LSLLILPLFLPVLIFAAAVLEAATLNMGYSGQLAIIGAILAVTLTFSPFAIAGALRIS
                     LN"
     gene            complement(10817..11458)
                     /gene="ccmA"
                     /locus_tag="PXE44_000961"
     CDS             complement(10817..11458)
                     /gene="ccmA"
                     /locus_tag="PXE44_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDL5588486.1"
                     /translation="MNQTAQYQLKLNQIACERGETRLFEGCSFTVQSGEWVQIEGHNG
                     IGKTSLLRILAGLFLPAEGEVLWNDIAIQKQRDHYYSELFYLGHYSGVKPELTPWENL
                     RFYQKIQQLPQDDDALWHALTKVSLIGREDLPCSHLSAGQQRRVALAKLWLTNAKLWI
                     LDEPFTAIDKKGVADLIAHIEQHCEQGRLVIFTSHQAAESDKVRIISLDQFKV"
     gene            complement(11602..12312)
                     /gene="hda"
                     /locus_tag="PXE44_000962"
     CDS             complement(11602..12312)
                     /gene="hda"
                     /locus_tag="PXE44_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HDL5588487.1"
                     /translation="MQLPLPIHQIDEDTFDNFYSQNSEVLLGSLKRNFTDVQQPFFYI
                     WGGKSCGKSHLLKAVSNHFLLNNQTSSYIPLTKANYFSPLVLDNAELLNVICLDDIQA
                     IAGDAEWELAIFNLFNQIREQQGLFNEGQKTLLLISADCPPHQLNIQLPDLRSRLTWG
                     EVYQLNDLTDEQKCQILQLNAHQKGVELSDEVAHFLLKKAGTDLEQLSKILDKLDKAS
                     LQAQRKLTIPFVKEILSL"
     gene            12582..14183
                     /locus_tag="PXE44_000963"
     CDS             12582..14183
                     /locus_tag="PXE44_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5588488.1"
                     /translation="MKNMTKLSLITLALAASSSVFAAPKTFVYCLEASPSALSPVLST
                     DGASFDVNALPIYNKLTDFEDGTTNVIPSLAEKWDISEDGKTYTFHLRKGVKFHDNKE
                     FKPSRELNADDVVFSINRQFDPNHPFHKVSGGNYEYFIGMDMQNIIDKVEKVDDHTVK
                     ISLKVPNAPFLANLAMDFNAVYSAEYAEQSLKAGKPERIDTNPIGSGPFQFVDYQKDA
                     TVRYKAFENYWQGTAKIDRLVFAITPDASVRLAKLQKGECHAMPYPNPADIEDLKKDP
                     NIELMSQSGMNIGYLALNSSIKPLDNQKVRQALNHAVNKQAIVDAVYQGAGQVAKNPI
                     PPTMWSYNEAVQDYDYNPEKAKALLKEAGFENGFDTELWAMPVSRPYNPNARRMAELI
                     QEDWKKVGVNAKIVSYEWGEYLKRLRQGEAATSMIGWTGDNGDPDNFLNTLLSCSAVE
                     AGSNYAKFCHKPFEEVVLGAAQESDKAKRTELYKQAQVIFKEQAPWITIAHSTVYFPV
                     RKEVKGYVMSPFSLHNFYKVDLADK"
     gene            14455..15459
                     /locus_tag="PXE44_000964"
     CDS             14455..15459
                     /locus_tag="PXE44_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5588489.1"
                     /translation="MFSFILKRVLMVIPTFIAITLITFALVHFIPGDPIEIRMGERGL
                     SPEVHQQMMEQLGLNRPLFEQYISYIKGVLHGDLGNSFRNNEPVLKEFFTLFPATVEL
                     AFFALLWSLIFGIILGVIAAVKKESWISHTVTTLSLTGYSMPIFWWGLILILYFSTPL
                     GLPASGRLPSEYWIEAETGFMLIDTWNSDEPGAFLAAIKSLILPSIVLGTIPLAVVTR
                     MTRSSMLEVLGEDYIRTAKAKGLNTTRIVIVHALRNALIPVVTVVGLIVGQLLSGAVL
                     TENIFSWPGIGKWIIDAINSRDYPVLQGSVLIIATIIILVNLTVDLLYGIVNPRIRHS
                     "
     gene            15561..16454
                     /locus_tag="PXE44_000965"
     CDS             15561..16454
                     /locus_tag="PXE44_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5588490.1"
                     /translation="MSGITAVNPPAPKTPLQEFWYYFCQNRGALIGLTFIAIVFVACV
                     FAPWVAPFDPIEQNRSALLLPPAWLEGGNSAHLLGTDDIGRDILSRIIYGSRLSVFIG
                     LVIVLLSCVFGVILGLLAGYYGGTLDIIIMRFVDIMLAIPSLLLTIGVVTILGPSLIN
                     AAIAIAIVSIPSYVRLTRASVLSEKNRDYVTASRVAGASVLRLMFVVILPNCLAPLIV
                     QMTMGISNAILELAALGFLGIGAQPPTPELGTMLAESRGFMQSANWLVTIPGLAILSL
                     VLAFNLMGDGLRDALDPKLKQ"
     gene            16465..17454
                     /gene="dppD"
                     /locus_tag="PXE44_000966"
     CDS             16465..17454
                     /gene="dppD"
                     /locus_tag="PXE44_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDL5588491.1"
                     /translation="MALLTVDQLSVHFGETKTPFKAVDRISYEVNEGEVLGIVGESGS
                     GKSVSSLAIMGLIDFPGRVMAEKLYFNGNDLLALKPKEKQQIVGADVSMIFQDAMTSL
                     NPSYTVGYQIMEALKVHQGGSKASRRERAIELLTMVGIPDPESRLEVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIDLLLELQRKENMALILITHDLALVA
                     ESAHRIIVMYAGQVVEEGKAEQIFKAPLHPYTQALLKALPEFAEGKSRLQSLPGVVPG
                     KYDRPQGCLLNPRCPYATEKCRTIEPAMQIINGRQVKCHTPLNAQGEPIRIAV"
     gene            17582..18571
                     /locus_tag="PXE44_000967"
     CDS             17582..18571
                     /locus_tag="PXE44_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5588492.1"
                     /translation="MKENNSLPPFLKAVDLKKYYPVKKGMFAKPKTVKALDGVSFTLE
                     RGKTLAIVGESGCGKSTLGRMLTMIESPTEGELFYNGQNFLVNDKETAQLRRKKIQIV
                     FQNPYSSLNPRKKVGSILEEPLLINTNLSAKERKEKVLEMMAKVGLKPEFYDRYPHMF
                     SGGQRQRIAIARGLMLQPDIVVADEPVSALDVSVRAQVLNLMMDLQEEMGLSYVFISH
                     DLSVVEHIADEVMVMYLGRCVEQGDTKTIFSNPRHPYTQALLSATPRLNPEQRRERIK
                     LTGELPSPLNPPKGCAFNARCRFATDRCYVERPELKTYQDGTRIACFVVEENN"
BASE COUNT         5685 a         4148 c         3945 g         5099 t
ORIGIN      
        1 acttttacta ctatttattt cacaatcaaa aaaggaatca gactatgagc caacttgaca
       61 ccttaagcaa aatgaccgtt atcgttgctg acactggtga tattgaagca atcaaacttt
      121 ataagccaaa agatgcaaca acaaacccgt ctttaatttt aagtgcatcg gcattaccgc
      181 aatatgctcc attaattgaa gaagcgatta attacgcaaa agcacaaagt agcgataaag
      241 atcaacagtt agttgatgct gaagataaat tagcggtaaa tattggttta gaaatcttaa
      301 aacttgtacc tggacgtatt tctacagaag ttgatgctcg tttttcttat gatactgaaa
      361 aaactatcgc taaagcacgt aaattaattg gattatataa cgatgcaggc atcagtaatg
      421 atcgcatttt aattaaaatt gcctctactt ggcaaggtat aaaggcagca gaacagttag
      481 aaaaagaagg cattaactgt aacttaacgt tattattctc tcaagctcaa gctcgtgcgt
      541 gtgcagaagc aggggtttac ttaatctctc cattcgttgg tcgtatttta gactggtaca
      601 aagctcacga aggtaaagaa tatgctccag ctgaagatcc aggtgtaatt tcagtaacca
      661 atatatacaa ttactacaaa caacacggtt atgacactat tgtaatgggt gcaagtttcc
      721 gtaatgtagg agagatcatt gagttagctg gttgcgaccg tttaaccatc tcaccaccgt
      781 tattaaaaga gctacaagag agcgatgaaa aattagtttg caagctagat tacaaaggtg
      841 aaatcaagcc tcgtccagaa aaaatgacag aagcagaatt ctactgggag cataaccaag
      901 agccaatggc agtagaaaaa ttagctgaag gcattcgtaa atttgcgatt gaccaagaga
      961 agttagaagc aatgcttgct gaaaaacttc aatagacaac gcataaaaaa accggcatta
     1021 acgataatta atgccggttt taatttctat aaatgattat ttaaactcat aacgtctaat
     1081 cgtttttaga tcgctatctt catttttaat ttcatcggca agatgaacac gagcaccaac
     1141 cgctgcatgt gcatcatact cacgagttag agtcacagtt tcaccattat ctcgttgaac
     1201 tgtaagttca acaacatcgt ttgatactgc tttgctagat aaatgttgca ttacctttat
     1261 cagaataaac atctgcagta caagctgcta acataagtga agctaataca gctaaagaaa
     1321 gttttttcat taataatata tcctctagga aaaactaaaa ttaaagcaga gaaacattca
     1381 ggtctgaatt agaccctacg attctcacac gtttgccagg cacaaaaccg tcttctttct
     1441 tttgaacgac cacgatttct ttaccatcat ctttgcgaat aaccatttcc aatgaagaaa
     1501 cttgactcgc tttttgttct acttgattac caattaccgc accggcaact gcccccactg
     1561 cggttgcaat cgcttgccct gtaccaccgc caatcgcatt accggcaacg ccacctaata
     1621 caccaccgcc gacagtaccg atcacacctt cactgcgtgc ttgaattttc acatcacgca
     1681 ctgaaacaat agtgccataa gcgatagaac gagcttcttt tgcctgctca ccggaataaa
     1741 cgcttccgct aaacacgtct gtatttgcac agccaactaa agcaaaactt gacattaatg
     1801 cgacagctaa agctaatttt ttcattttgt ttgttctccg aaacgttaat tttctgacat
     1861 aattacactt tttatatcat aagggtggag ctttagacaa taaggattac aaaaagttac
     1921 atttctcaag ctgatcgcaa tgcttggatt tggcaatctc tccccttcta cctgaggttg
     1981 gctgattttt aatttaatct cagaattatc agcaagtcta aagtgcgaca atgcccttga
     2041 cacccattgt tccgaacttt cgttgtctaa catcggaaaa accagctcaa tatgttccca
     2101 gccttcttct aaataaacct tatttttcgg aaaaggcaac tcgacaatct ttacaaattg
     2161 tccgcaaaat tcaaccgctt gtattaatgc aaacagccct atcggtctgc cgtttacctc
     2221 gctctcttta agtaaggttg ccccatcaag caaaaggatc ttccactgtt ctgcggtttc
     2281 ggtttggttc attcttaccg caagatgatc gatttcatac gctgctaaat caatgccggc
     2341 gatttcagca atttgtcgga ttttctgttc aaattgcgat aaatcgccaa aacaagcggt
     2401 cattttctcg aaaaattttg cattttctgc tataattttg tccatttttt tgtaaataga
     2461 atttaaatag gttaaacagt gaatattcaa caaattttat cagataaaat taaacaggca
     2521 atggtagcaa gcggtgcaga cagcaatgtc gagcctttag tccgccaatc gggcaagccg
     2581 gaatttggcg attaccaagc caatggtgcg atgggtgcag cgaaaaaatt ggggatgaat
     2641 ccgcgtgaat ttgcccaaaa aatcttagag aatgccgatt tgaacggtat tgccgataaa
     2701 ttagaaattg ccggtccggg ctttatcaac attttcttaa acccagaatg gctggcggag
     2761 aacactaaca gttcattgca agcggtcaat tttggcataa aaactgcaaa tccgcagacg
     2821 attgtgatcg actactcctc gccaaacgta gcaaaagaaa tgcacgtcgg gcatttgcgt
     2881 tccaccatta ttggcgatgc ggtagttcgt acccttgcgt ttttaggcaa taatgtgatt
     2941 cgtgccaacc acgttggcga ctggggtact caattcggta tgctgatcgc ttaccttgaa
     3001 aaaatggaaa atgagcacgc cagcgaaatg gagctaagcg atttagaagc cttctatcgt
     3061 gcggcaaaag agcattatga ttcagacgaa gcattcgccg aaaaagcccg taactatgtg
     3121 gtgaaattac aaagcggcga taaatattgc ttaacaatgt ggaaaaaatt ggtcgatatt
     3181 acaatgcacc acaatcaaga gaactacaat cgcttaaatg ttacgctcac cgaaaaagat
     3241 gtaatgggcg aaagcctcta caatccaatg ttgcctgaaa ttgtggcgga tttaaaagcc
     3301 aaaggcttag cggtggaaga tgacggtgct ttagtggttt tcctcgatga atttaaaaat
     3361 aaagatggcg acccaatggg cgtaatcgtg cagaaaaaag atggcggttt cttatatacc
     3421 actaccgata ttgcggcagc aaaatatcgc tacgagacct taaaagctga ccgagcctta
     3481 gtcttttctg acagccgcca agctcaacat atgcaacagg catggctcat tacccgcaaa
     3541 gcgggctatg tgccggacag tttcagcctt gaacacccgt tcttcggaat gatgctaggc
     3601 aaagacggca aaccgttcaa aacccgtagc ggtggcacag tcaaactcaa agatttatta
     3661 gatgaagcgg tagagcgtgc cgataaactg attgccgaac gttcaaccga gctaactgcg
     3721 gaagaaaaag ccgccgtggt tgaagcggtt gcgattggtt cggtgaaata ttctgactta
     3781 tcgaaaaacc gcaccaccga ttatgtgttc gattgggaca atatgctgac ctttgaaggc
     3841 aataccgccc cgtatatgca gtatgcctac acccgcattc gctccatctt tgcccgtgcg
     3901 aacattgacg aaaatgcatt gagcggttca atccaactca atgagccaaa agagcgtgca
     3961 ttagcagtga aactattgca atttgaagag gcattaaacg gcgtggcaaa agaaggtatg
     4021 ccacatattt tatgccaata tttatacgag cttgcaggta gtttctccag tttctacgag
     4081 gcttgcccga ttctcaatgc cgaagaaaca accaaaaaca gccgtttgaa actcgctgcc
     4141 ttaacggcga aaaccttgaa acaagggttg gatttactcg gtattaaaac tgtagagaag
     4201 atgtaataca aactctctcc ccttgtggga gagagaccgc ttatgagcgt tcagcgaaat
     4261 aagcagagag aggggacaag cggtaatttt tattaaaatt tttacacatt ttcccctctc
     4321 cctgattttt cttctgaacg aagaaaaatc tgtccctctc ccacaagggg agagggcata
     4381 taaaaggaaa caaatatgaa atttattgac gaagccctga ttcgtgtaga agcaggcgat
     4441 ggcggtaatg gctgtgtgag cttccgccgt gaaaaattta tcccgaaagg cggacctgac
     4501 ggtggtgatg gtggcgatgg tggtgatgtg tatttaattg ccgatgaaaa cctaaacacc
     4561 cttatcgact accgctttga aaaacgttat gccgctgggc gtggcgagaa tggtcgcagt
     4621 gccggctgta ccggacatcg tgggcaagat attaccctac gagtgccggt tggaacccgt
     4681 gcgattgata acgacaccca agaaattatt ggcgatttaa cccgccacgg aatgaaaatg
     4741 ctggtggcaa aaggtggtta tcacggttta ggtaataccc gctttaaatc ctcagtaaac
     4801 cgagctccac gccaaaaaac caacggtacg cctggcgaaa aacgtgattt gaaattagag
     4861 ctgttactgc ttgccgatgt gggaatgctt ggcttaccaa acgccggtaa atccaccttt
     4921 atccgcagtg tctctgctgc caaaccaaaa gtggcggatt acccgttcac gacgctggtg
     4981 ccaagtttag gggtagcccg tgtgggggcc gaccgcagtt ttgtggttgc cgatattccg
     5041 gggttaattg aaggtgcagc agatggtgca gggctgggga ttcgtttcct aaaacacttg
     5101 gaacgttgcc gtattttgat tcacttagtc gatattatgc cgattgatga aagcgatccg
     5161 gctcataata tttcggtgat tgaatccgag ctttaccaat acagcgaaaa actcgccgaa
     5221 aaaccgatct ggttggtgtt taataaaatt gacaccatcg gcgaagaaga agctgctgag
     5281 cgagcaaaag acattgccga gcaaatcggc tgggaaggcg attactactt gatttctgcc
     5341 gctaccggtc aaaacgtgca agctctcact cgtgatatta tggactttat tgaagccaac
     5401 ccacgcgaag tggaggaaga aaataaagca cctgaagaag tcaaattcaa atgggaagac
     5461 taccacaacg aagcaatgca aaatgcggta gaagaagatt gggacgacga ttggagcgaa
     5521 gaagatgacg aaggcataga gtttatttac gaacgccatt agtgcaataa tacaagcggt
     5581 tgtttttaaa cggagatttg caaatttccg gagaaaaaca accgcttgta ggtttaagga
     5641 gagtttatgc ttagctcaac atcgtgcgat ttattcaata ttccgttctt ccaatttgct
     5701 cagatgaaga aatattgccc tgaggagatc cctcgaatta aggcggaata taaaactcaa
     5761 tgggaaattt ggaaaaagct acatcaggaa gtttctcgtc agcttgggca gccgtttgct
     5821 ccaccacata ttgagaagtg gtgcaacggt tggcaagttc gagcccattt ttttgcctat
     5881 tacaagtatg aatttaatcg aaattctgcg gctattcttt ccgtgatttt aaatcgtcgc
     5941 cgtttgcaag tgagccttga ttggcattgt tatcgagccg atcgctcgca aattaacgtg
     6001 catcaataca atcaatggtg ggaaaattta gatcgggaaa agtttgctga ttttgagatt
     6061 tggcacggaa gtgagagtga atatgatgat tttagaaccg ttagtcagat gtcagagcaa
     6121 gcgttcaccc taactaacga ggaagatttt tggtgtatcg gcaaaaatgt ggaaaaggac
     6181 gatttggata acattgatgt agtagaattt atctaccaaa ccattcgggc attattgcca
     6241 ctttatgaga aatgtcatca ataaacacta aaaagagagg cgaacatcat ttgttcgccc
     6301 ctttcaaaat ttaagctatc acttcttttt tattttcaat gcgtaaacgg tagcgtttat
     6361 ccaacataca gagtaaacca ccgagagcca taaacagccc gccaatccaa atccagcgaa
     6421 tgaacggttt ataatacaaa cgcactgccc aagagttatc ttctaatttt tcgccaagtg
     6481 cagcgtataa atctcgggta aagccccagt caatcgccgc ttcggtcatt cccattcggc
     6541 tcaccgtata gaaacgtttt tcagcattaa gtgtggcttc atatttaccg tttcgagtaa
     6601 tttcaagctc ggcagtgccg ccttgatagt ttgccccatc ggtgatttta atacctttaa
     6661 attcaaattg gtaatctaaa atctcagctt tatcacccac attcattctg acatctcgtt
     6721 caatgctgaa attttggcta aatgcaatgc cgaacactgt catcgcaacc cctaagtgag
     6781 ccagcaccat tccccaatgt gaacgggaca gtttgaaaat gccacttaag aacgagtggc
     6841 gatgggttgc acgttggtgt agctcataca ggctaagtaa cacaataaag acagacatca
     6901 tcgtgcctaa caccacactc acggtaatgg tgttacggaa taagtatggc aatccgaatc
     6961 caagcaatgc cattaaaata gttgcaataa ttaccggttt acgaatagca gaaacttggt
     7021 ctctacgcca tttcactaat ggtccaatcc ctaatacaaa tgagaaagga accattaaaa
     7081 ttaagaacat ttggtcaaaa aatggtgccc cgattgaaat cgtccctaag ccgatttgtt
     7141 tgtgaattaa tggcagtaag gtccctaaaa acaccaccga taaaaatgcc attaataaaa
     7201 tattattaat gagtagcatc gtttctcttg aatagcgttc atagttatct aagcttttaa
     7261 tttccgagcc tttgaatgca tacagcagta aagaaccacc tactactaac accaagtaag
     7321 ccaagatgta taaaccacga gttggatctg aagcaaaggc gtgaactgac accaaaatgc
     7381 ctgaacgcac taagaaagtc cccactaaac agagcgagaa agcaagaatc gctaataaga
     7441 ccgtccacgc tttaaatgtg cctcgttttt cagtcactgc taatgaatga atcagtgctg
     7501 ttcctgcgag ccacggcatt aatgaggcat tctcgacagg atcccagaac caccagccac
     7561 cccaaccaag ttcgtagtat gcccaccaag aaccaagcac gataccgagc gttaaaaata
     7621 cccacgctgc cattgtccac ggacgagacc atcttgccca cgcagtatct aaacgccctg
     7681 tcatcagtga ggcaatcgca aaagcaaagg caacggagaa acccacatag cccatataaa
     7741 gcaacggtgg gtggaagatc aagcccacat cttgcagcat tgggtttaat tctttgccat
     7801 caatcggaaa atttgggaaa gttctatcaa acgggttgga ggtaaaaatc acaaacatca
     7861 aaaagccgat gccaatgacc cccattagtc ctaacactcg agcaacggca tcttccggca
     7921 ttttacgggt aaaggttgct accccgacag accaaagcga caacaaccaa acccaaagca
     7981 ataatgagcc ttcgtgagcg ccccacaccg cagataaacg atattgcaac ggtaaacggc
     8041 tattggagtt gttcaccaca tattgcacgg taaaatcatt caccgcaaac agataaaaca
     8101 gcgaaccaaa agagacagtc aatgccaaaa acattgccca agtcatcggg cgggcaagag
     8161 caattaaggt gctattttgc ttttctgctc cccaaagcgg taaaactgcc aacaagatag
     8221 aaatcgctag ggctaatgcc aacgtataat tgcctaattc tgcaatcatt tgctttcccc
     8281 ttgcacattg ttggcttttt ctgcttccgc ctcggctttc attttgcggt cggtttccga
     8341 ttcacctttc agatcttgtt cagacacacc tgatttttta tgctgttctt ttaatttatc
     8401 gcctaattcc ggtggcatat agttttcatc gtgttttgcc agaacttccg tagcttttaa
     8461 acgggttggt tcaatcaaca cgccttgtgc cacaatgcct tgcccttcac ggaataaatc
     8521 cggcaaaatt ccttcgtatt ccacggtaat cgaaggcccg atatcattga gatcaaatac
     8581 tacttttaaa cttttatcat cacgctttac cgtatcttcc actaccattc cgcccacacg
     8641 gatacgctga ccaacttcag gcttttgttt cggatcatca tttttaccgt ttacaatttc
     8701 agacggcgtg taaaacagat cgatattttg gcttaacgca taaagtgtca agcctgctgc
     8761 gacagaaagc ccaagtaata tcgaaaccac cacttttaat cttgatttgc gtcttggatt
     8821 catcgtaatc acccttcaaa ataaatgcaa aattgcataa aaactcaaaa ataaattagt
     8881 gtaagaatag caaaaaaccg caaaacattc ccgatatttc aaggaagttt tgcgatttct
     8941 caaatcaaat acctataaag aagtatgagg ctatcgttgc aaaaaataaa gttatttcga
     9001 ccgcttgttt attgtgatgt ttgctttaat ttttcttcac gcacaatctc tttttttgct
     9061 tgagccaaaa tcgctttctg ttcacggcga ctaagccaaa tcagcccaat cattgcaatg
     9121 aaactgacac cataagagag ccatacataa aagccgtagc cacccattgc aaaaaaatcg
     9181 ctgattgatt caaattgaaa tgtcattgtt ctctcctact ctaaactgat tttgccagtt
     9241 gttttaccca agggcgatta cgttcgtcct ttaataaggc aatgcgataa cgccaaatgc
     9301 taaaccaagc ggtaaaaatt aagctaccga aaatcgccaa tagcaacgga attaacattt
     9361 ccaccgccat agacggttta tcgaatttag tgatggtcgc cccttgatgc aaggtgttcc
     9421 accattccac cgaaaagtga ataatcggca agttaatcac gccaacaact gacagcacag
     9481 ccgccgcttt ggctccggtt tgtctgtctt ggaatgcgga ataaagagcc atcacgccga
     9541 tataaaggaa aaataacact aaagcggagg ttaaacgagc gtcccacacc caccaagtcc
     9601 cccacattgg tttgccccaa attgcaccgg ttgcaagtga aataaaagcg aatactaagc
     9661 cgataggtgc cattgaaatc atcgccaagt tagcttggcg aatttgccaa accaatgcca
     9721 ccaagcctgc caccgccatt gagccatata cgcccatcga ccaaattgct gccggcacgt
     9781 gaataaagat aatgcgatag ctatcgccct gttggtaatc tttcggggca aaagccaagc
     9841 cccaaacaaa agcagccgct aacataatca aacttagcca acctaaaaac ggttggattt
     9901 tccctagcaa atggtactgc gtttccgatt ttgcataagg gtgtagccat ttccacatat
     9961 atttctccaa agtaaataat tcccctcacc cgtttacagg agagggacga tttgagcttc
    10021 gttcagaagc gaaaatcagg gagaggggaa aaagttgcaa attttcataa taattcgacc
    10081 gcttcccctc tctctgaaaa acacctctga acgaggtgtt tttcggtctc tctccctcaa
    10141 gagggagaga ggaagtttta gttcaaacta attcttaatg caccggcaat cgcaaacggc
    10201 gaaaaagtta aagtaaccgc taaaattgct ccgataatcg ccagttgtcc gctataaccc
    10261 atatttaagg tggcggcttc taataccgca gcggcaaaaa tcagtaccgg taagaataac
    10321 gggaggatta ataaactcag caacgtaccg cccttgcgta agcccacagt gagtgccacg
    10381 ccaatcgctc ctaagcagct taaaatcggg gtgccgagta ataatgtcag cactaacgcc
    10441 caccaaacgt gggtttcaag tgaaagcaaa atggcagcaa tcggtgaaag taaaatcaac
    10501 ggtaagccgg tcagcagcca gtgagcaatc actttcgcta acgcaacctg cggcaaggca
    10561 agcggcagta acattaattg ctcgagcgaa ccgtctaaat aatcatcacg gaataaccgc
    10621 tcaaaggaaa gcaatgcaga aagcaaagca gctacccacg caatgccgcc ggcaattttg
    10681 cctaataatt cggggttagg tcccattaaa atcgggaata cggtaatcac cattaaaaaa
    10741 aaccacaacg gatttaaaat ctccgagggt ttacgaaagg caatggtgag ttcccgctga
    10801 ataatagtca ataaaatcat actttaaatt gatctaacga aataatacgc actttgtcgc
    10861 tttcagcagc ctggtggctg gtaaaaatca ctaatctgcc ttgctcgcaa tgctgttcaa
    10921 tatgagcaat cagatccgcc acaccctttt tatcaatagc ggtaaagggt tcatctaaaa
    10981 tccaaagttt ggcgttagtg agccacaatt ttgccaacgc cacacggcgt tgctgaccgg
    11041 ctgacaaatg cgaacaaggc aaatcttcac gcccgatgag cgacactttc gtcaatgcgt
    11101 gccaaagggc atcatcatct tgcggtaact gttggatttt ctggtagaaa cgcaaatttt
    11161 cccacggtgt taattccggt tttacccccg aataatgccc taaataaaat agttcagaat
    11221 aatagtgatc acgctgtttt tgaattgcaa tatcgttcca cagcacctca ccttccgcag
    11281 gtaaaaaaag cccggctaaa atacgtaaca ggctggtttt tccaatgccg ttatgccctt
    11341 caatctgcac ccattcacca ctttgcacgg taaaactgca cccttcaaac aaacgggttt
    11401 cgccccgttc gcaggcaatc tgattaagtt taagctgata ttgggccgtt tggttcatta
    11461 tttttcatct ggttgaacat tttagccgaa gtgtatcata agcctttgat ttaacgtagc
    11521 aaataaatta cttctttaga ggtagcaagc ggtcaaattt tcaaaaagtt ttgcaaattt
    11581 tgataagaat caaaccgctt gttacagaga gaggatttcc ttcacaaaag gaatggttaa
    11641 tttacgctgg gcttggagag acgctttatc caacttatcc aatattttag agagttgctc
    11701 taaatccgtt cccgcttttt ttaacagaaa atgagcgacc tcatcggaca attccacccc
    11761 tttttggtga gcatttaact gtaaaatttg gcatttttgt tcatcggtta aatcgtttag
    11821 ctggtacact tcgccccaag ttaagcggga acgtaaatcc ggcaattgga tattgagttg
    11881 gtgtggtggg caatcggcac tgattaacag caaggttttt tgcccttcgt taaacaagcc
    11941 ttgttgttct cgaatttggt taaacaggtt aaaaatagca agttcccatt cagcatctcc
    12001 tgcaatcgct tgaatatcat ctaggcaaat cacattcagc agttcggcat tatctaacac
    12061 caacggcgag aaataattgg ctttagtgag cggaatataa ctagaagttt ggttatttaa
    12121 aagaaagtgg ttacttactg ctttcaacaa atgactttta ccgcagctct taccgcccca
    12181 aatgtaaaaa aacggctgtt gaacatccgt aaaatttcgt ttgagggatc caagcaacac
    12241 ttcgctgttt tggctataaa aattgtcgaa agtgtcttca tcaatttgat gaatgggtag
    12301 aggtaactgc aaggctttcc ttaaatctct aagtttgata tttttctaag aatagcggaa
    12361 aagcaggggt ttgagaagta aaaatagtga gaaaatatcc actcattttt ctaatgaaga
    12421 gatgacaagc ggttgaattt ccacataatt ttgtcaatct gaccgaaaac ccaccgtttt
    12481 gtgttaaaaa aagattgcag ttagctcaaa aaattgtata ttcaaagctt tctacttaaa
    12541 aagtagagta aaacaactat tacaaacaca acggagtgaa tatgaaaaac atgactaaat
    12601 tatcgctaat aacattagcg ctagccgctt caagtagcgt ctttgctgca ccgaaaacct
    12661 ttgtttactg cttagaggct tcgccttcag cattaagccc agtgctatca acagacggtg
    12721 caagctttga tgtgaacgcc ctcccgattt ataacaaatt aaccgacttt gaagacggta
    12781 caactaatgt tattccctct cttgccgaaa aatgggacat ttcggaagac ggcaaaactt
    12841 acaccttcca cctgcgtaaa ggcgtaaaat tccacgacaa taaagaattt aaaccaagcc
    12901 gtgagttaaa tgcagacgat gtagtgttct ccattaaccg tcagtttgat ccaaaccatc
    12961 cattccacaa agtttccggc ggtaactacg aatactttat cggtatggat atgcaaaata
    13021 ttatcgataa agtggaaaaa gtggacgatc acaccgttaa aatcagctta aaagtgccaa
    13081 atgcgccgtt cttagctaac ttagcgatgg atttcaacgc cgtttactca gcggaatatg
    13141 cagagcaatc cttaaaggcc ggcaagcctg agcgtattga taccaaccca atcggcagcg
    13201 gcccgttcca gtttgtggat taccaaaaag atgccaccgt tcgttacaaa gcctttgaaa
    13261 actactggca aggcacagcg aaaattgacc gcttagtgtt tgcaattact ccggatgcat
    13321 cggtacgttt agccaagtta caaaaaggcg agtgccacgc aatgccttac cctaacccgg
    13381 ctgatatcga agatctgaaa aaagatccaa atattgaatt aatgagccaa tctggaatga
    13441 atatcggcta tttggcatta aattcatcga ttaaaccatt agataaccaa aaggtacgtc
    13501 aagcattaaa tcacgctgta aacaaacaag cgattgtaga tgcggtatat cagggagccg
    13561 gtcaagtagc gaaaaaccca attccaccaa caatgtggag ctataatgaa gcggttcaag
    13621 actatgacta caaccctgaa aaagccaaag cattgttaaa agaagccggt tttgaaaatg
    13681 gttttgatac tgaactttgg gcaatgccgg tatcacgtcc atataacccg aatgctcgcc
    13741 gtatggctga acttatccaa gaagactgga aaaaagtggg cgtgaatgcg aaaatcgtca
    13801 gctatgaatg gggcgaatat ctcaaacgct tacgccaagg tgaagcggca acctcaatga
    13861 ttggctggac aggcgataac ggtgacccag ataacttcct caacacctta ttaagctgct
    13921 cagcggtgga agccggttca aactacgcca aattctgcca caaaccgttt gaagaagtcg
    13981 tgcttggtgc tgcccaagaa agcgacaaag caaaacgtac cgagctttat aaacaagctc
    14041 aagtgatctt caaagagcaa gctccttgga ttacgattgc tcactccacc gtttacttcc
    14101 cggtgcgtaa agaagtaaaa ggttatgtga tgagtccgtt cagcttacat aacttctaca
    14161 aagtagattt agcggataaa taatccgcca acaagcggta gaatttgcag atttttttac
    14221 aaatttgacc gcttgtcctc ccctctttga actacgaggg gcagggggga aatttggcag
    14281 aagcgattgc cgaagtgaga ggattgtaaa agaggaaata aattttctgt cttattgatt
    14341 attttcttcg ttaattacta ctgtcaaatc tcccctaccc cctctttgct aaagagggga
    14401 atcagatcga gagatcagac aactttgatg aaaatccaac ctaagagatt ttaaatgttt
    14461 tcatttattt taaaacgtgt gctaatggtg atccctacct ttatagcgat tacgttaatt
    14521 acttttgccc ttgtgcattt tatccccggt gatccaattg agatccgaat gggggaacgt
    14581 ggattatcgc ctgaggttca tcaacaaatg atggaacaac tcgggctgaa ccgaccgttg
    14641 tttgaacaat acatcagcta catcaaaggt gtattgcacg gtgatcttgg taactcattt
    14701 cgtaacaacg aaccggtgct gaaggaattt tttaccctat tcccagccac ggttgagttg
    14761 gcattttttg ccctgctctg gtcgttgatt ttcggcatta ttctcggtgt gattgctgcg
    14821 gtgaaaaaag aaagttggat ttcacacact gtaaccacgc tttcactgac cggctactca
    14881 atgccaattt tctggtgggg cttgatttta attctttatt tctccacccc gctcggctta
    14941 ccggcttcag gtcgtttacc atctgaatac tggattgaag ccgaaaccgg ttttatgctg
    15001 attgatactt ggaattcgga cgaaccaggg gcatttttag cggcaattaa atctctgatt
    15061 ttgccgtcta tcgtactggg gacaatccct cttgcagttg tgacccgaat gactcgctct
    15121 tcaatgttgg aagtgttggg tgaagactat atccgcaccg ccaaagccaa aggcttaaac
    15181 actacccgaa ttgtgattgt tcacgcatta agaaatgcct tgattccggt ggtaacggtc
    15241 gttggcttaa ttgtcgggca actgctttca ggagccgttt taacggaaaa catcttctcg
    15301 tggccgggta tcggcaaatg gattattgat gcaatcaaca gccgtgatta ccctgtacta
    15361 caaggctcag tgctgattat tgctactatt attattttag tgaaccttac ggtagatcta
    15421 ctctatggca ttgtcaatcc gaggattcga catagttaga aaaattcctt cgatttttcc
    15481 cctctttagc aacgagggag ttacacaagc ggttcaaatt ccgcaatttt ttacaagatt
    15541 atttttagtt ggagttaatg atgtcaggaa taacggcggt aaatccaccc gcacccaaaa
    15601 caccgttaca ggagttttgg tactattttt gtcaaaatcg gggggcatta ataggactta
    15661 cctttattgc aatcgtcttt gttgcgtgcg tattcgcacc ttgggttgcc ccttttgatc
    15721 caatcgaaca aaatcgcagt gcgttattgc tccctcctgc ttggcttgaa ggggggaatt
    15781 ccgctcatct gctgggtact gacgatattg ggcgtgatat tctttcccgt attatttatg
    15841 gttcaagatt gtccgtcttt atcggcttag tcattgtgct actttcttgc gtattcggcg
    15901 tgattttagg tctacttgct ggctactatg gtggcacact cgacatcatc attatgcgtt
    15961 ttgttgatat tatgctggcg attccaagcc tgcttttaac tatcggcgtg gtcactattt
    16021 taggcccttc attgataaat gccgctattg cgattgcgat tgtctccatt ccaagttatg
    16081 ttcgcttgac ccgtgcttct gtattaagcg agaaaaaccg tgattatgtt actgcttctc
    16141 gtgttgctgg tgcgagcgtg cttcgcttaa tgtttgtggt gattttacct aactgtttag
    16201 ctccgcttat cgttcagatg acgatgggga tctcaaacgc tattttagaa cttgccgccc
    16261 tcggcttctt aggcattggt gcacagccgc caacaccgga gcttggcaca atgcttgccg
    16321 aatctcgtgg ctttatgcaa tcggcaaact ggctggtgac cattccgggt ctagcgattt
    16381 tatcgttagt attagctttc aacttaatgg gtgacggctt gcgtgacgcc ctcgatccaa
    16441 aactgaaaca ataggagatc taaaatggca ttattaacgg ttgatcaact ttccgtccat
    16501 tttggtgaaa ctaaaacacc atttaaagcg gtggatcgta tcagttacga agtcaatgaa
    16561 ggcgaagtat taggcattgt gggcgaatcc ggttcaggaa aatcggtaag ttctctcgct
    16621 attatgggct taatcgattt ccccggcagg gttatggctg aaaaattgta ttttaacggc
    16681 aatgatttat tggcattaaa accaaaagaa aaacaacaga ttgtaggggc tgatgtctca
    16741 atgatcttcc aagatgcaat gaccagctta aatccgagct atactgtggg ctatcagatt
    16801 atggaagccc taaaagtgca tcaaggtggc tcaaaagcca gccgtcgtga gcgagcgatt
    16861 gagttgctga cgatggtcgg tattcccgat cctgaatccc gtttagaagt ttatccgcac
    16921 cagctttcgg gtggtatgag ccagagagtg atgattgcga tggcgattgc ctgtcgcccg
    16981 aaattattga ttgccgatga gccgactact gcgttagatg tgacgattca agctcaaatc
    17041 attgatttat tacttgaatt acaacgcaaa gaaaatatgg cgttgatttt aatcacgcac
    17101 gatttagcgt tagttgccga gtctgcccat cgcattatcg tgatgtatgc agggcaagtc
    17161 gtggaagagg gcaaagccga gcagatattc aaagcaccac tgcaccctta cacccaagca
    17221 ttgctcaaag ccttaccgga atttgccgaa gggaaatccc gcctgcaatc actaccgggc
    17281 gtagtgccgg gcaaatatga ccgcccacaa ggttgcttac tcaaccctcg ctgcccgtat
    17341 gcaaccgaaa aatgccgaac cattgaaccg gcaatgcaga taatcaacgg cagacaggtg
    17401 aaatgccaca cgccactgaa tgcacagggt gagccgataa ggatagctgt ataacgtcat
    17461 aaagagtagg gacgcaatgc ttcgcgtccg ttacaagcgg tgatttttaa ccgatttttg
    17521 caaaataacg atagtggacg caaagcattg cgtccctaca ttttccgaga aggaaaacag
    17581 aatgaaagaa aataattctc tcccaccatt cctcaaagca gtggacttaa aaaaatacta
    17641 cccagttaaa aagggaatgt tcgctaaacc gaaaacggta aaagcgctgg atggtgtctc
    17701 ttttaccctt gaacgtggca aaacccttgc gattgttggc gaatccggct gtggaaaatc
    17761 aactttgggg cgaatgctca ctatgattga aagcccaacc gaaggtgaac tgttctacaa
    17821 cgggcagaat ttcttagtca atgacaaaga aacggctcaa ctacgccgta aaaaaatcca
    17881 aattgtgttt cagaacccgt atagctcgct caatccacgc aaaaaagtag gtagcatttt
    17941 agaagaacca ttattgatta acaccaactt atccgcaaaa gaacgcaaag aaaaagtgtt
    18001 ggaaatgatg gcgaaagtcg gcttaaaacc ggaattttac gaccgttatc cgcatatgtt
    18061 ttcgggcgga caacgccagc gaattgcgat tgctcggggc ttaatgttgc agcctgatat
    18121 tgtggttgcc gatgaacccg tttccgcatt agatgtttcg gttcgggcgc aagtgttgaa
    18181 tttgatgatg gatttgcaag aagaaatggg cttgtcttac gttttcatct cgcacgattt
    18241 atccgtagtg gaacatattg ccgatgaagt gatggtgatg tatttagggc gttgcgtaga
    18301 acaaggggat accaaaacta tcttcagcaa tccacgtcac ccttatacac aagcgttact
    18361 ctccgcaacc cctcgtttga acccggagca acgccgtgag cggattaaac tgactggcga
    18421 actaccaagc ccgcttaatc caccaaaagg ctgtgccttt aacgcccgtt gcagatttgc
    18481 aaccgatcgt tgctatgtgg agcgaccaga gctgaaaacc tatcaagacg gtactcgcat
    18541 tgcttgtttt gtggtggagg aaaataatta ggcaacgctc gctatttaaa aggttaaagt
    18601 ttttcaactt taacctttat ttttcatact taatactcct catcttttgt agcaaaatct
    18661 ttcattaatt tgctgatatg ctcacaatgg tgtttaaatg acaatacggg caaataatca
    18721 aatgcgtttt aacgccaatc ttctctttta aagtgagttt tcgttccttt gagtcagaaa
    18781 ttaaccttgt tacttgtcgg caattcatct atttctctcc gttaatttcc ttgaaagaca
    18841 ttgttgtaac tgcagccttg ctcgatagat taatgta
//
LOCUS       DANLZC010000036        18795 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.036, whole genome shotgun sequence.
ACCESSION   DANLZC010000036 DANLZC010000000
VERSION     DANLZC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18795
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.036"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            11..2446
                     /gene="plsB"
                     /locus_tag="PXE44_000968"
     CDS             11..2446
                     /gene="plsB"
                     /locus_tag="PXE44_000968"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDL5588493.1"
                     /translation="MSGLLNFYRAFLNLPLSLLVKSRSIPTHPVAELELNLEQPIVYV
                     LPYTSQTDLLILQKNCLALNLPDPLQENVIEGQTLLRFVFLDEGRRFFKSKGAKSETE
                     SIFYRYLDLHRANAELDVQLVPVSVLWGRAPGKEDARHLQVLTSFQRFLSMVWFGRDN
                     FVRFSPAVSLRYMVTEYGADEKIAQKIARVAKMHFAKLRYSAMGPRLPNRDAMFNKIL
                     NSEVIQAAIAEEAKKSSPEKARKEAEKIINEIAADVKHESLRVADRVLSWLWNKLYQG
                     INVQNADRVRKLALEGHEIVYVPCHRSHMDYLLLSYLLYHQGLVPPHIAAGINLNFWP
                     AGPIFRSWGAFFIRRTFKGNRLYSTIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEP
                     KTGMMSMSLQALQRGLNRSLSIVPVYIGYEHVLEVDTYAKELRGAAKEKENAGLVLRV
                     LKKLKNLGQGYVNFGEPILVNNYLNQYFPEWKEPSEDGRPKWLNEAVDNISHQVMVNI
                     NKAAAVNAKNLVGSALLASRQRALTREQLIEQLGSYIQLFRNVPYSKDMTLPTESAEV
                     MLNHVIHLPRSGVLIEKDNFGELVRLERESAVLMTYYRNNVQHLFVLPSLVASMVLHH
                     EAVSKDVVIKSVNRIYPFLQAELFLHFKQEELKAHIEAIIAELSRQGVIKNESDMLMI
                     NRSHIRTLQLHSAGVREILQRYYISLSVLLEQPNISRGYLEKESSSIAQRLSVLHGIN
                     APEFFDKAIFSTFTSSLKAQGYFDGDDNLAIAKVKETESLLRSLISIEVQQTIQGAMA
                     KVAESVENPNIAG"
     gene            complement(2551..3114)
                     /locus_tag="PXE44_000969"
     CDS             complement(2551..3114)
                     /locus_tag="PXE44_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDL5588494.1"
                     /translation="MSAPNSGTNPAPLGLCGFALTTFLLSLINNGTFSGENVGLVLAM
                     GFAFGGTSQMIAGMFEFAKGNTFGFTAFTSYGAFWWSFALFKVFFAANVSAEFVGWYL
                     VAWGTFTLMMFIGTLAKARALQAIFLSLTITFYLLAAGDFTHNTTLTNIGGNFGLLTA
                     FLAFYLAAADIINESFGKTVLPIGEPK"
     gene            complement(3312..4208)
                     /locus_tag="PXE44_000970"
     CDS             complement(3312..4208)
                     /locus_tag="PXE44_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5588495.1"
                     /translation="MDILDRLLAFAQISAGISVKCQLQGDWQLENPHQTGQAIAHIIS
                     YGQAYLQHNGKTHHLKQGDIVFFPRFADHTLRSQIQPLTSKNAVNPEQLGAFTLTQAG
                     LSQSECDMFCLHFYYDHQAELMESLPEMIVFQVAETPLKAMLDILKQEAETPNLAVKS
                     VVNSLAVVILTMILREHLVNHNNQLFGILKGWQDLHLRPLIKAILLNPEHEWQVDDMA
                     AFIHLSRAQLVRLFNKHLCISPHAFVHKIRLQKAAMLLKSQAGSVLSIALSCGFQSES
                     HFITAFKKQYCVTPSQYRQLNP"
     gene            4344..4685
                     /locus_tag="PXE44_000971"
     CDS             4344..4685
                     /locus_tag="PXE44_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL5588496.1"
                     /translation="MSSFQNWTEHTAHVKQSFGKLGQKHPKMLEAYKALGTAAAAEAL
                     DHKTRELIALAVAITTRCESCISVHAEEAAKAGATESEIAGALATAIALNAGAAYTYA
                     LRALEAVEEQK"
     gene            4696..5049
                     /locus_tag="PXE44_000972"
     CDS             4696..5049
                     /locus_tag="PXE44_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL5588497.1"
                     /translation="MKKLAWILGFAAMMPMMSSAKQASVQQVQAEKAAGIWIDVRSAE
                     EFQQGHLNGAINVTHTEIAQRIAEVAPDKNQPIHLYCRSGHRAEAALNELKKLGYSNV
                     TNHGGYDDLIKAGLK"
     gene            5109..5747
                     /locus_tag="PXE44_000973"
     CDS             5109..5747
                     /locus_tag="PXE44_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgB family protein"
                     /protein_id="HDL5588498.1"
                     /translation="MSAFNNFVNFVANIVAPMQRQFINFVRIAICIVMVWIGGLKVCQ
                     YEADGIAHFVSNSPFFSYMYKKGPNLVDDGTGKMVMEYTLHKNPEGKMVAKNIEWHKE
                     NGTYTASYIIGATIVTVGLLTLSGIWFPVSGMAGGLLTFGMSIVTLSFMITTPEIWVP
                     NLGGDMPTPAHGFPYLSAVGRLIVKDVIMMAGGLVAAAECANRILARRKAIA"
     gene            5882..7063
                     /gene="purT"
                     /locus_tag="PXE44_000974"
     CDS             5882..7063
                     /gene="purT"
                     /locus_tag="PXE44_000974"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDL5588499.1"
                     /translation="MTTIGTPLRTNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRY
                     DNAPAQQVAHRAYTISMLDGNALRDLVEKEKPDFIVPEVEAIATATLVELEQEGYNVI
                     PTAKATQLTMNREGIRRLAAEELGLKTSPYRFVDNFEQFQQAIQEIGIPCVVKPIMSS
                     SGHGQSVIKSEADIQQAWDYSQQGGRAGGGRVIVEGFIKFDYEITQLTVRHIHGTSFL
                     APIGHIQVDGDYRESWQPQQMSDIALKKAQETAEKITSALGGRGIFGVELFVCGDEII
                     FNEVSPRPHDTGIVTMASQELSQFALHARAILGLPIPEIYRISPAASKAIVVEGKSDN
                     VRFGGVDKVLAEIGTNIRLFGKGEVNGHRRLGVILARDENTVRALEKARRAYDKLDIQ
                     L"
     gene            complement(7155..8648)
                     /locus_tag="PXE44_000975"
     CDS             complement(7155..8648)
                     /locus_tag="PXE44_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL5588500.1"
                     /translation="MFKQLQQVGKAFMLPIAILPAAGLLLGIGGALSNKATVQAYPIL
                     DNPTLQGIFQIMASAGSVVFGNLALLLCIGLGIGLAKRDKGVAALAAVVGYLIMTGTI
                     SALIPLFPPETKGIDTGVIGSLVMGLITVKLHNKYHNIQLPQILGFFGGSRFVPIVTA
                     FAAIFVGAVFFLIWPAFQQWLVSAGQGIAEMGEIGTFFYGFLMRLSGAVGLHHMIYPL
                     FWYTELGGTEVVNGETIIGAQKIFFAQLADPTHQGLFTEGTRFFAGRFDTMMFGLPAA
                     CLAMYHAVPKARRGAISGLFLGAALTSFITGITEPIEFMFLFVAPWLYVFHAFLDGVS
                     FYIADLLNISIGNTFSGGVIDFTLFGILQGNENTNWLRVIIVGLPWAALYYFSFLFLI
                     KKFNVMTPGRAEESEEVVEQQTSSLTENAHKIIAALGSANNIENVDACITRLRVAVKE
                     VKAVDKATLKKLGAIDVLEVGGGVQAVFGAKAVLYKSEVNQILGKED"
     gene            8849..10696
                     /gene="ilvD"
                     /locus_tag="PXE44_000976"
     CDS             8849..10696
                     /gene="ilvD"
                     /locus_tag="PXE44_000976"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDL5588501.1"
                     /translation="MPILRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFT
                     QFVPGHVHLKDMGQLVAAEIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLI
                     RLDLVDAMIESADPNVSDERIDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSMLATHADRKELFLRAGREIVELCRKYYQQDDESVLPRSIATFDAFENAMSLDIAMG
                     GSSNTVLHLLATAQEAGVDFKMEDIDRLSRVVPCLSKIAPNTNKYHMEDVHRAGGIMG
                     LLGELDRAGLIHRHVKTVLGLTIEEQLNQYDIIRNKDEDLHKFFRAGPAGIRTTKAFS
                     QDCRWDTVDDDRVNGCIRNVENAVTKEGGLAVLFGNIAEDGCIVKTAGVDESIWKFTG
                     TAIVFESQEDAVAGILGGKVKEGHIVIIRYEGPKGGPGMQEMLYPTSYLKSMGLGKKC
                     ALLTDGRFSGGTSGLSIGHASPEAASGGAIALINDGDTVAIDIPNRSINLMVSDEELA
                     ARRAEMEARGDKAWQPLKREREVSFALKVFGHFATSADKGAVRDKSLLK"
     gene            complement(10830..11180)
                     /locus_tag="PXE44_000977"
     CDS             complement(10830..11180)
                     /locus_tag="PXE44_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HDL5588502.1"
                     /translation="MWHFYLMTIELNNIQYPTDASGYLTNLNDWSVELAEKIAEKEQI
                     SLTAEHWEIIYLVRDFYQEYKTSPAIRMLVKTMAQKFGEEKGNSRYLQRLFPDGPAKQ
                     ATKIAGLPKPAKCL"
     gene            complement(11209..11874)
                     /locus_tag="PXE44_000978"
     CDS             complement(11209..11874)
                     /locus_tag="PXE44_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor protein"
                     /protein_id="HDL5588503.1"
                     /translation="MENRLAHSYSTGESLISTHKVLRNTYMLLAMTLTFSAVVAFVAI
                     QMNAPAMPWWGLLVGFYGLLFLTNATSNSSAGILSVFALTGFLGYTLGPILNRYIGAG
                     LGDIVALAFGATALVFFACSAYVLTTKKDMSFISGMMVALFVVLLVGIIANIFLAIPA
                     LSLALSSLFVIFSSGAILLATSNIIHGGETNYIRATVDLYVSIYNLFVSFLQIFGVLG
                     SDD"
     gene            12080..12628
                     /gene="pgsA"
                     /locus_tag="PXE44_000979"
     CDS             12080..12628
                     /gene="pgsA"
                     /locus_tag="PXE44_000979"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL5588504.1"
                     /translation="MKLNFPTYLTLFRVGLTPLFIVAFYLPTKYAPEISTLIFFIASI
                     TDAFDGYLARKWNQTTRLGAFLDPVADKVLVAVAFVCVAEYYHTWWITIPICIMIARE
                     IIISALREWMAELGERASVAVSIWGKVKTTAQMLALGGMLWRQNQLMEILAFILLYIA
                     VGLTIWSMMQYLNASKNSLLKS"
     gene            12706..14544
                     /gene="uvrC"
                     /locus_tag="PXE44_000980"
     CDS             12706..14544
                     /gene="uvrC"
                     /locus_tag="PXE44_000980"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL5588505.1"
                     /translation="MSEFNAKSFLLNVPHQPGVYRMYDSKGTIIYVGKAKDLKKRLAS
                     YFRSQLSSKKTEALVANLANIETTITHSETEALLLEHNYIKENQPKYNVLLRDDKSYP
                     YILLTKHKHPRLTAFRGSKKIAGEYFGPYPNVTAVRETLNLLQKIFPIRQCEDTYYKN
                     RSRPCLQYQIGRCLAPCVEGCYSPVEYDQQVNLVRLFLQGKDHQVVEHLVQKMEDAAE
                     ALDFEGAARFRDQIQSVRAVQEKQFVSNDRLDDLDIISIVYQNGIACVHILFVRQGKV
                     LGNRSYFPKVPSNTDLTELADTFVGQFYLQMNQHRTIPNQIVIDHQLSESKALSEILS
                     EQAGHKVSILDNAIRGDKQRYLALARTNAMAALVLQLKQESHIQSRYQALKEMLKMSE
                     IKRMECFDISHTMGNQTVASCVVFDENGPLKSDYRRFNIEGITGGDDYAAMEQALLKR
                     YNRELPPEKIPDIIFIDGGKGQLNRALDVFAKLDVEWDKSKPLLIGVAKGVERKAGLE
                     TLIISKWDKEIHLPPDSLALHLIQHIRDESHQHAISGHRKKRQKAFTESGLESIAGVG
                     AKRRQALLKYLGGMQGVKSATLEEIQSVPGISKALAEVIFDTLKNS"
     gene            complement(14549..14824)
                     /locus_tag="PXE44_000981"
     CDS             complement(14549..14824)
                     /locus_tag="PXE44_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588506.1"
                     /translation="MKKLILLGLSVALTACSTPQQQSTVPMDMQAVQDYHQRVINKQT
                     VDKNTKQHDEPLNQSDKRSESINLPIHPIIYPSIGVGYHYGWKRYRY"
     gene            complement(14949..15302)
                     /gene="rplT"
                     /locus_tag="PXE44_000982"
     CDS             complement(14949..15302)
                     /gene="rplT"
                     /locus_tag="PXE44_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL5588507.1"
                     /translation="MARVKRGVIARARHKKVLKAAKGYYGARSRVYRVAFQAVVKAAQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGLSYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFAALVEKAKSAL"
     gene            complement(15411..15608)
                     /gene="rpmI"
                     /locus_tag="PXE44_000983"
     CDS             complement(15411..15608)
                     /gene="rpmI"
                     /locus_tag="PXE44_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL5588508.1"
                     /translation="MPKIKTVRGAAKRFKKTASGGFKRKQSHLRHILTKKTTKRKRQL
                     RHKSMVAKADQVLVVACLPYA"
     gene            complement(15872..16414)
                     /gene="infC"
                     /locus_tag="PXE44_000984"
     CDS             complement(15872..16414)
                     /gene="infC"
                     /locus_tag="PXE44_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL5588509.1"
                     /translation="MKPVKRVQSNRAHRLNDEIRGVKEVRLTDENGEQVGIVSLQDAL
                     NRAEEAELDLVEISPNAEPPVCRIMNYGKFIYEKEKAAKEQKKKQKVVQVKEIKFRPG
                     TDDGDYQVKLRSIIRFLEDGDKVKITVRFRGREMAHQEIGMDVLERVKADTAEIAMVE
                     SAPGKLEGRQVVMVIAPKKK"
     gene            complement(16573..18372)
                     /gene="recQ"
                     /locus_tag="PXE44_000985"
     CDS             complement(16573..18372)
                     /gene="recQ"
                     /locus_tag="PXE44_000985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL5588510.1"
                     /translation="MNTTAISVLNNIFGYQKFRDGQEEVINAVLENRDCLVIMTTGGG
                     KSLCYQVPALCLDGITLVISPLISLMKDQVDQLITNGIEAAYLNSTQTLEEQQLVEQK
                     ALSGQLKLLYLSPEKVMTQGFFHFISLCKISFIAVDEAHCVSQWGHDFRPEYTLLGGL
                     RNTFPNVPLMALTATADLTTRSDILHHLRLNSPHIYLGSFDRPNIRYTVQEKFKPLEQ
                     LIKFISKQQGKSGIVYCNSRKKVEEITEKLSARKISVMGYHAGMSFEQRERVQNAFQR
                     DDIQIVVATIAFGMGINKSNVRFVVHFDLPRSIESYYQETGRAGRDDLPSEAVLFYDP
                     SDYAWLQKILLEEPESEQRNIKQHKLQAIGDFAESQTCRRLVLLNYFGEHRQEQCKNC
                     DICLDPPQQYDGLLDAQKVMSVIYRTGQIFGQHHIIAVLRGMNNLKIREHQHDQLSVY
                     GIGKEQSQHYWQNVVRQLIHLGLIIQNIANHSALQLTESARPFLRGEKQLQLALPRLG
                     ISATAFVQKQASHRYDKDLFARLRFLRKQIADLENIPPYIVFNDATLQEMAEFLPTSK
                     VEMLEINGVGERKFERFGQAFMSLIKEYQNNRK"
     gene            complement(18481..18786)
                     /gene="cyaY"
                     /locus_tag="PXE44_000986"
     CDS             complement(18481..18786)
                     /gene="cyaY"
                     /locus_tag="PXE44_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDL5588511.1"
                     /translation="MNTAQFHQAVEQVWQQIEEKIYDEGLSVDTEIQGAVCTLTFDDE
                     SQVIINKQEAMLELWLASKLGGFHFKNVEGEWVTDEGRSFWQHLEEAFARHGESVSF"
BASE COUNT         5453 a         3593 c         4125 g         5624 t
ORIGIN      
        1 ggatatttat atgtctggct tattaaattt ttatcgagct ttcctaaact taccactttc
       61 acttttagta aaatctcgct ctattccgac tcatccggtg gctgaactgg aactaaatct
      121 tgaacagccg attgtctatg ttctacctta tacctcgcaa accgatttat taattttaca
      181 aaaaaactgc ttggctttga atttgcccga tccgttgcaa gaaaatgtga ttgaagggca
      241 aacattgctt cgttttgtat ttttagatga aggtcgccgt ttttttaaat ctaaaggggc
      301 gaaaagtgag acagagtcga ttttttatcg ttatttagat ttacatcgtg ctaatgctga
      361 attagacgtc caattagtgc cggtttcggt attatggggg cgggctccgg gcaaagaaga
      421 tgcccgccat ttacaggtgc ttacctcatt ccaacgtttt ttatccatgg tttggtttgg
      481 gcgtgataat tttgtacgtt tctcccctgc agtatcgtta cgctatatgg taactgaata
      541 tggtgcagat gaaaaaatcg ctcaaaaaat cgcgcgtgtg gcgaaaatgc actttgcaaa
      601 attgcgttat tcagcaatgg ggcctcgttt accaaatcgt gatgcgatgt ttaataaaat
      661 cctgaattca gaggtgattc aagctgcgat tgcggaagaa gcgaagaaat catcgcctga
      721 aaaggctcgc aaagaagccg agaaaatcat taatgaaatt gccgctgatg taaaacacga
      781 gagtttgcgt gtagcagatc gagttctgag ctggctttgg aataagcttt atcaagggat
      841 caacgtgcaa aatgcagacc gtgtgcgtaa attggcatta gaaggtcacg agatcgttta
      901 tgtgccttgc caccgtagcc atatggacta tttgctccta tcttatctac tttaccatca
      961 aggattagta ccgccacata ttgccgctgg gatcaattta aatttctggc cggcaggccc
     1021 gattttccgt agttggggag catttttcat tcgccgtacc tttaaaggaa atcgcctcta
     1081 ttccaccatt ttccgtgaat atttagcgga attattctat cgtggttatt cggtcgaata
     1141 ttttattgaa ggcgggcgtt cacgcacagg tcgtttatta gagcctaaaa ccggtatgat
     1201 gtcgatgagc ctacaagcct tacagcgagg gctgaataga tctttaagta ttgtgcctgt
     1261 ttatatcggt tatgaacacg tcttagaagt cgatacttac gctaaagagt tgagaggtgc
     1321 ggcaaaagag aaagaaaatg cgggcttggt gctacgagtg ctgaaaaaac tgaaaaattt
     1381 aggtcaagga tatgtgaatt tcggtgagcc aattttggtg aataattact taaatcaata
     1441 tttccctgag tggaaagagc cttccgaaga tggacgacct aagtggttaa atgaagccgt
     1501 ggataatatt tcacatcaag taatggtgaa tatcaataaa gcggcagcag ttaatgcgaa
     1561 aaacttagtg gggtcagcat tattggcatc tcgccagcgt gcattaaccc gtgaacaact
     1621 cattgaacaa cttggaagtt atattcagtt atttcgtaat gtaccttatt ctaaagatat
     1681 gacattacca actgaaagtg cagaagtgat gttgaatcat gttattcatt tacctcgttc
     1741 gggcgtttta attgaaaaag ataattttgg tgaactggtt cgtttagaac gagaatctgc
     1801 tgtgttaatg acatattaca gaaataatgt gcagcattta tttgtgttgc cttcacttgt
     1861 tgcaagtatg gtacttcatc atgaggctgt ttcgaaagat gttgtgatta agtcggttaa
     1921 tcgtatttat ccattcttgc aagcagagtt attcttacac tttaagcaag aagaattaaa
     1981 agcacatatt gaagcaatta ttgcggaact aagtcggcag ggggtgatta aaaacgaaag
     2041 tgatatgtta atgatcaatc gctcacatat tcgtaccttg caattacatt cagctggtgt
     2101 gcgtgaaatc cttcaacgtt attatattag cctaagtgtc ttgttggagc agccaaatat
     2161 tagtcgtggc tatttagaaa aagagagtag ttcaattgct cagcgtttat cagtcttgca
     2221 tggtattaat gcgccggagt tttttgataa ggcgattttc tctaccttta catcaagtct
     2281 aaaagcacaa gggtattttg atggtgatga taatttagcg attgcaaaag ttaaagaaac
     2341 ggaatcgttg ttacgtagtt tgatttcgat tgaagttcaa cagaccattc aaggtgcgat
     2401 ggctaaagtc gcagaatctg tagagaatcc taatatcgca ggataataaa tcaaagaagg
     2461 gcgctaacaa gcgctcgttt cttgcaattt tgttgccaaa aataaaagct gaatgcgcga
     2521 ccacattcag cttttttact tcctatataa ttattttggt tcaccgattg gtaaaacagt
     2581 cttaccaaaa ctttcattaa taatatccgc agcggctaag tagaacgcta agaatgcggt
     2641 taataaaccg aagttaccac caatgttagt tagggtggtg ttatgggtaa aatcacctgc
     2701 ggctaaaagg tagaaagtaa tggttaaact taagaaaata gcttgtaatg cacgggcttt
     2761 tgctagtgtg ccgatgaaca tcataagtgt gaatgttccc caagctacta agtaccaacc
     2821 aacgaattct gccgatacat tcgcagcaaa gaacacttta aataatgcaa aagaccacca
     2881 gaatgcacca tagctggtaa acgcagtaaa gccgaacgtg ttacctttag caaattcaaa
     2941 catacctgca atcatttgtg atgtgccacc gaaagcaaat cccattgcta aaactaaacc
     3001 tacgttttca ccgctgaatg taccgttatt gattaggctt agtaaaaatg tagtaagtgc
     3061 aaaaccgcat aaaccgagcg gagccgggtt agttcctgaa tttggagctg acattgaaaa
     3121 cctctgttat attgttacac cataagtgtg gtaattgttt atttacgccg ataaatcaga
     3181 cgttatgtgc ctaaaattta gggcgataaa gtatagcgat tttgcagtaa attgactaga
     3241 aatttccgag acaagcggtg agatttttgc aaaattttgc caaaaaatga ccgcttgttg
     3301 ttgtcattta gctatggatt taactgccga tattggctgg gtgttacgca atattgcttt
     3361 ttaaaggcgg taataaaatg cgactcggat tggaagccgc aagataaggc gatacttaat
     3421 accgacccag cttggctttt gagcagcatt gctgcttttt gtagcctaat tttgtgtaca
     3481 aaagcatgtg ggctaataca taaatgtttg ttaaataagc ggactaactg ggcacgagaa
     3541 aggtggataa atgccgccat atcatcgact tgccattcgt gttcgggatt aagcaaaata
     3601 gctttgatca aaggtcttaa gtgtaagtct tgccaacctt ttaaaatacc gaataactga
     3661 ttgttatggt taactagatg ttctcgtaag atcatagtta aaataacaac agcaagcgag
     3721 ttaaccacag attttacggc taaattaggt gtttctgctt cttgttttag aatatctaac
     3781 attgctttta atggtgtctc ggcaacttga aatacgatca tttcaggtaa gctttccatt
     3841 aattcggctt gatggtcata ataaaaatgg aggcagaaca tatcgcattc tgattgagaa
     3901 agtcctgctt gggtgagtgt aaatgcaccc aattgttcag ggtttaccgc atttttgctg
     3961 gtcaaaggct gtatttgact acgtaatgta tgatcggcaa atctggggaa aaatacaata
     4021 tctccctgtt taaggtgatg ggttttccca ttatgttgta gataagcttg cccatagctg
     4081 atgatatgag cgattgcttg tccggtttga tgcggattct caagttgcca atctccctgt
     4141 aactgacatt tcacactgat tccggctgag atttgagcga aagctagaag cctatctaat
     4201 atatccattg ttcattcctt atatttccac cttaaattgc gattatttca ttataatatg
     4261 agctgatttg ataataaagt ttatcagata aacgcaataa taaatgcgtt ttttcatatt
     4321 attcacttta ggaggaaatc aatatgtctt cattccaaaa ttggacagaa cacacagctc
     4381 acgttaagca atcttttggt aaattagggc aaaaacaccc taaaatgctt gaagcttata
     4441 aagcattagg tacagcggcc gcagcggaag cattagatca caaaactcgt gaattaattg
     4501 cattagcagt agcgattaca acacgttgtg aaagttgcat cagtgtacac gcagaagaag
     4561 cggcaaaagc aggtgcaacc gaaagcgaaa ttgccggtgc attagcgact gcgattgcat
     4621 taaatgccgg tgcggcatat acctatgctt tacgtgcatt agaagcggtg gaagagcaaa
     4681 aataaggagc aataaatgaa gaaattggct tggattttag gctttgcagc tatgatgcca
     4741 atgatgtcta gtgctaaaca agcatcagtt cagcaagtgc aggctgaaaa agcagcgggc
     4801 atttggattg atgtacgttc tgctgaagaa tttcagcaag ggcatttaaa tggtgcaatt
     4861 aatgtaacgc atactgaaat tgcacaacgt atcgcagaag ttgctccaga taaaaatcag
     4921 ccgattcatc tttattgccg ctcgggtcat cgagctgaag cggcgcttaa tgagctaaaa
     4981 aaattaggtt acagcaatgt aactaatcat ggtggttatg acgatctgat taaagccggg
     5041 ttgaaataac tgttttgagt gccaagcatt tgcttggcac ttttcgttta tatctgaggt
     5101 taaaaattat gagtgctttt aacaattttg ttaattttgt tgcaaatatt gttgctccaa
     5161 tgcaacgcca attcattaac tttgtacgta ttgccatttg tatcgtgatg gtttggattg
     5221 gtggtttaaa agtctgccaa tatgaagcag acggtattgc ccattttgtg tctaatagcc
     5281 cgttctttag ttatatgtac aaaaaaggcc cgaatttagt tgatgacggc acaggtaaaa
     5341 tggtgatgga atataccctt cacaaaaacc ctgaaggtaa aatggtggca aaaaacatcg
     5401 aatggcataa agaaaacggc acttatactg catcttatat catcggtgca accattgtga
     5461 cggttggttt attgacgtta tccggtattt ggttcccggt ttccggtatg gcggggggct
     5521 tactcacctt tggtatgtcg attgttacac tctcttttat gatcaccacc cctgaaattt
     5581 gggtgccgaa tttaggtggg gatatgccaa ccccagcgca tggtttccca tatctgtcag
     5641 cagtcggtcg tttaattgtg aaagatgtga ttatgatggc aggcggcttg gttgccgcag
     5701 cggaatgtgc aaatcgcatt ttagccagac gtaaagcgat tgcttaattg atagtggtaa
     5761 tgcaagcggt cattttcttg taaaattttg caataaaatg accgcttgtt ttttgtcaat
     5821 ttgccataat ttcaagtata attcaattac gtaatcgttt gcttttatgg agaggaataa
     5881 aatgacaaca atcggtacac cgttaagaac gaacgctaca aaagtgatga tgttaggttc
     5941 gggcgagttg gggaaagaag tggtaataga gctgcaacgc ttaggcgtag aagtgattgc
     6001 ggtggatcgt tatgacaatg cacctgcaca gcaagtggct caccgtgctt atacgatttc
     6061 gatgttagac gggaatgcat tgcgagattt ggtggagaaa gaaaaaccgg attttattgt
     6121 gcccgaagta gaagccatcg caactgcaac cttggttgag ttagagcaag agggatataa
     6181 cgtaatccca acggcaaaag caacgcaatt aacaatgaat cgagaaggca ttcgccgatt
     6241 ggcggcagaa gagttgggct taaaaacgtc gccttatcgt tttgtggata attttgaaca
     6301 gttccagcaa gcgattcaag aaatcggcat tccgtgtgtg gtcaaaccga ttatgtcttc
     6361 ttccggacac gggcaatcag taattaaatc tgaagcggat attcaacagg cttgggatta
     6421 ttcgcaacag ggcggacgtg ccggcggtgg gcgtgtgatt gtagagggtt ttatcaaatt
     6481 cgattatgaa attacccagc ttaccgttcg ccatattcac ggcacgtcgt ttttagctcc
     6541 catcgggcat attcaggttg atggcgatta tcgtgaatct tggcaaccgc agcaaatgtc
     6601 ggacattgca ttgaaaaaag ctcaagaaac cgcagaaaaa attacctctg cactgggcgg
     6661 aagagggatt ttcggcgtgg aactttttgt ctgtggcgat gagattattt ttaacgaagt
     6721 ctccccacgt ccacacgata ccggaatagt aacaatggca tcacaagagc tttctcaatt
     6781 tgctctgcac gcccgtgcga ttttgggctt accgattccg gaaatttacc gcatcagccc
     6841 ggcagcttct aaagcgattg tggttgaagg gaaatcggat aatgtgcgtt ttggcggtgt
     6901 agataaggta ttagccgaaa tcgggacgaa tatccgctta ttcggtaaag gcgaagtaaa
     6961 cggacaccgc cgattagggg taattcttgc tcgtgatgaa aatacagtga gagcattaga
     7021 aaaagctcgg cgagcttatg ataagttaga tattcaatta taaagtcagg attttgatat
     7081 tcgttgatag taggcatttt atgcctacta tcctataggc ctctaatcaa attggatgaa
     7141 aaagttgaag attgttaatc ctctttgccg agaatttggt tcacttcgct cttatacaat
     7201 actgcttttg ctccaaatac ggcttgtaca ccgccaccga cttccagcac gtcaatcgca
     7261 ccgagttttt tcagtgttgc tttatccacg gctttcacct ctttcactgc cacacgcaga
     7321 cgggtaatac aagcatccac attttcgata ttatttgcag agccaagagc ggcgataatt
     7381 ttatgggcat tttcagtcag tgagctggtt tgttgttcga ctacctcttc actctcttcc
     7441 gcacgtcccg gtgtcattac gttgaatttt ttgatcagga ataggaacga gaagtagtaa
     7501 agtgccgccc acggcaagcc gactatgatc actcgaagcc agttagtgtt ctcgttgcct
     7561 tgcaaaatgc caaacagggt gaagtcgatc acgccaccgg agaaggtgtt accaatcgag
     7621 atgttgagta agtctgcaat gtagaacgac acgccgtcta aaaaagcgtg gaacacatag
     7681 agccacgggg caacaaacag gaacataaat tcaatcggct cggtaatgcc ggtgataaaa
     7741 gaggtgagcg ctgcacctaa gaacagaccg ctaatcgcac cacggcgggc tttcggcaca
     7801 gcgtgataca tcgctaaaca ggcggcaggt aagccaaaca tcatcgtatc gaaacgtcca
     7861 gcgaagaaac gtgtaccttc ggtaaataag ccttgatggg ttggatcggc gagttgggcg
     7921 aagaagattt tttgcgcccc gataatggtt tcaccattta ccacttctgt tccgcccaat
     7981 tcggtgtacc aaaacagcgg gtagatcata tggtgtaagc cgaccgcacc gcttaaccgc
     8041 attaagaaac cgtagaaaaa ggtgccgatt tcgcccattt ccgcaatccc ttgccctgct
     8101 gaaaccaacc actgttggaa agccggccag atcaaaaaga acactgcccc gacaaaaatc
     8161 gcagcaaagg cggtcacaat cggcacaaac cgagagccgc caaagaagcc gagaatttgc
     8221 ggcagctgaa tgttgtggta tttgttatgc agtttcaccg taatcaagcc cattaccagc
     8281 gagccgatca caccggtatc aatccctttg gtttcaggag gaaacagcgg aatgagggca
     8341 gaaatcgtgc ctgtcataat caaataaccg accactgctg ccagtgccgc tacgccttta
     8401 tctcgttttg ctaatccaat gcctaaaccg atacacagta ataatgcaag gttgccaaac
     8461 accacactgc ctgccgatgc cataatctgg aaaatgccct gtaaagtcgg gttatccagt
     8521 atcgggtagg cttgcaccgt tgccttattc gacaaagctc cgccaatccc taacagcaaa
     8581 ccggctgccg gtaaaatcgc aatcggcagc ataaaagctt ttccgacttg ttgcagttgt
     8641 ttgaacataa aatttctcct tttttatggt tgtatttgat ggtttatagc atactgaaat
     8701 ttagccgtat gaggtgtgat ataactcaca gatttgcaaa atttttagca aattcgaccg
     8761 cttgtaaaaa aattaccgct tgcggaaaaa ttgaagtaga attaagcatt tttttagcaa
     8821 acacaacata aaaaatttgg agaagattat gcctatttta cgttcagcaa cttccactca
     8881 gggtcgtaat atggcggggg ctcgtgcatt atggcgtgca accggtatga aagaaaatga
     8941 ttttggtaag ccgattattg cagtggtgaa ctcattcacc caatttgtgc cggggcacgt
     9001 ccacttaaaa gatatggggc agttggttgc ggcagaaatt gaaaaagccg gtggggtggc
     9061 aaaagaattc aacaccattg cggttgatga cggcattgca atgggacacg gcggaatgct
     9121 ttattcttta ccaagccgtg atttaattgc agacagtgtg gaatatatgg tgaatgctca
     9181 ctgtgctgat gcgatggtgt gtatctctaa ctgcgataaa atcaccccgg gaatgttaat
     9241 ggcagcaatg cggttaaata ttccgactat ttttgtctct ggtggcccga tggaagccgg
     9301 aaaaactaaa ctttccgatc aattaattcg cttggattta gtcgatgcaa tgatcgaaag
     9361 tgccgacccg aatgtgtctg atgaacgtat tgatgcgatt gaacgtagtg cttgcccgac
     9421 ttgtggttct tgttcaggta tgttcaccgc aaactcaatg aactgtttaa ctgaagcttt
     9481 aggcttaagt ttaccgggca atggttcaat gttggcaact catgctgacc gtaaagagct
     9541 tttcctaaga gcaggtcgtg aaattgtgga actttgccgt aaatattacc aacaagacga
     9601 tgagagcgtg ttacctcgct caatcgctac cttcgatgca tttgaaaatg caatgagcct
     9661 tgatattgca atgggtggtt caagtaatac cgtgcttcat ttactagcga ctgctcaaga
     9721 agccggcgtt gattttaaaa tggaagatat tgaccgcctc tctcgtgtcg tgccgtgctt
     9781 aagtaaaatt gccccgaata ccaataaata ccatatggaa gacgtacacc gtgccggtgg
     9841 gattatgggc ttgctgggcg aattagatcg tgcaggttta atccatcgcc acgttaaaac
     9901 agtgctaggt ttaacgatag aagagcaact gaatcaatac gatattattc gtaataaaga
     9961 tgaagattta cacaaattct tccgtgcagg tccggcagga attcgtacta ctaaggcttt
    10021 ctcgcaagat tgccgttggg acactgttga tgatgaccgt gtaaacggct gtattcgcaa
    10081 tgtggaaaat gctgtgacca aagagggcgg gttagcggta ttatttggga atattgcaga
    10141 agatggatgt attgttaaaa ccgcaggggt agatgagtct atttggaaat tcactggcac
    10201 cgcaatcgtg tttgaaagcc aagaagatgc ggtagcaggt attttaggcg gaaaagtgaa
    10261 agaagggcat attgtgatta tccgctatga aggtccgaaa ggcggcccgg gtatgcaaga
    10321 aatgctttat ccaactagtt atttgaaatc aatgggctta ggcaaaaaat gtgctttatt
    10381 aactgacggt cgtttctctg gcggtacctc agggttatct atcggacacg cttcaccgga
    10441 agccgcttca ggaggagcga ttgccttaat taatgatggc gatacggtgg cgattgatat
    10501 tccgaatcgt tcgattaact taatggtttc agatgaagaa cttgctgctc gccgtgcaga
    10561 aatggaagcc cgtggcgata aagcgtggca gccgctaaaa cgtgagcgag aagtgtcgtt
    10621 tgcactaaaa gtgtttggac attttgccac ctctgctgat aaaggtgcgg tgagggataa
    10681 atcgctgtta aagtaaataa attagaaaaa agaagcattt caataatata ttgatctgct
    10741 ctttattcag ctgaatattt aatctagctg aatggagagc agatcgatag cctaatttac
    10801 atctttttgt tttgatacag ggaaatgtat tacaagcatt ttgccggttt tggcagacct
    10861 gcaattttag tggcttgttt tgcaggacca tcaggaaaca agcgttgtaa ataacggctg
    10921 ttgccttttt cttcaccgaa tttttgtgcc attgtcttta ccagcattcg aattgctggt
    10981 gaagttttgt attcttggta gaaatcccgc accagataaa taatttccca atgttctgcg
    11041 gttaagctaa tttgctcttt ttcggcgatt ttttctgcta attctacaga ccaatcattt
    11101 aaattagtta aatagcctga agcatcggtt gggtattgga tattatttaa ttcaatcgtc
    11161 atcaaataaa aatgccacat aatgtggcat tcctcttaaa ctaaaacgct agtcgtcact
    11221 acctagcaca ccgaagattt gtaagaagct tacaaatagg ttgtagatag acacatataa
    11281 atcaacggtt gcacgaatgt agttggtttc gccaccgtga ataatgttgc tggttgccag
    11341 taaaatcgca ccgcttgaga aaatgacaaa caagctgctt agggctaaac ttaatgccgg
    11401 aattgctaag aagatattgg caataatacc gactaataat accacaaaca gggcaaccat
    11461 cattccgctg ataaagctca tatctttttt ggtggttaat acataagctg aacaagcgaa
    11521 gaaaaccaat gcggttgcac cgaatgcaag ggcgacaata tcccctaaac ccgcaccgat
    11581 gtaacggttt aaaataggac ctaaggtata gcctaagaag ccggttaaag caaatacgct
    11641 taagataccc gcactgctgt ttgaggtggc attcgtcaag aaaagtaagc cgtaaaagcc
    11701 gaccaataat ccccaccacg gcatagccgg tgcgttcatt tgaattgcaa caaaagcaac
    11761 aactgcggag aaagtcaggg tcattgccaa taacatatag gtattgcgaa gcactttgtg
    11821 ggtgctaatt agagattcac cagttgaata gctgtgagct aaacgatttt ccataaaaat
    11881 tccttctgtt tttgtgttta aatcggaaca tccgatggat taatatctat ggataaattt
    11941 ccataaatca agaagtaagg tagccgtaat ttattgtaaa gtcaatgcga ttttgtaaca
    12001 gaagagtaaa aaaaaacatt tagttgtgtt acacttaata ccactctact ttatattcct
    12061 tttcatcgat gaattcgcta tgaagctcaa tttcccaact tatttaacct tatttcgggt
    12121 aggattaacc ccacttttta ttgtggcttt ttatctgccg acaaaatatg cgcctgaaat
    12181 ttcaacactt atttttttta ttgcatcgat taccgatgcc tttgacggct atttagcacg
    12241 caaatggaat caaaccaccc gacttggtgc atttttagat cctgttgcgg ataaagtctt
    12301 ggtagctgtt gcgtttgtct gcgtggcgga gtattaccat acttggtgga ttactattcc
    12361 aatctgcatt atgattgccc gtgaaattat tatttcggct ttgcgtgaat ggatggcgga
    12421 gcttggggaa agggccagtg ttgccgtttc gatttggggg aaagtaaaaa cgactgctca
    12481 aatgctcgca ttaggtggaa tgttatggag acagaaccaa ctaatggaga ttttagcctt
    12541 tattctgctt tatattgcag tgggtttaac gatctggtct atgatgcagt atttaaatgc
    12601 ttcaaagaat agtctattga agtcataagc ggtcatattt ttattctttt ttgcaatttg
    12661 tagagtggaa atatcgctct acaattttca attttatcaa ctctaatgtc tgaatttaac
    12721 gctaaatcct tcctgttaaa tgtgcctcac caaccgggtg tttatcgtat gtatgatagt
    12781 aaaggcacga taatctatgt gggtaaagcc aaagatttaa aaaagcgttt agccagctat
    12841 tttcgtagcc aactttccag taaaaaaacg gaagccttgg tggcaaatct tgctaatatt
    12901 gaaacgacga tcactcattc tgaaacagag gcgttactcc ttgaacataa ttatattaag
    12961 gaaaatcagc cgaaatataa tgtgttgctc agagatgaca aatcttatcc ttatattctg
    13021 ttaactaaac ataagcatcc tcgtttaaca gcatttcgtg gtagtaaaaa gatcgccggt
    13081 gaatattttg ggccttatcc gaatgttacg gcagttcggg aaacactgaa tttattgcaa
    13141 aaaatttttc ctattcgtca gtgtgaagat acttattata aaaaccgttc acgaccttgc
    13201 ttacagtatc agatcggacg ctgtttagcc ccttgtgtag agggctgtta ttcacccgtt
    13261 gaatacgatc agcaggttaa tttggttcgt ttgtttttac agggaaaaga tcaccaagta
    13321 gtcgagcatc tggtacagaa aatggaagat gcggcggagg ctttggattt tgaaggtgcg
    13381 gcacgttttc gggatcaaat tcaatcagta agagcggtgc aagaaaaaca attcgtctct
    13441 aatgataggc tagatgatct tgatattatc tcgatcgttt atcaaaatgg gattgcctgt
    13501 gtgcatatct tatttgttag gcaaggtaag gtactaggca atcgttctta ctttccaaaa
    13561 gtgccaagta atacagattt aaccgagctc gcagatacct ttgtcggtca gttttattta
    13621 caaatgaacc agcaccgcac catacctaac caaattgtaa ttgatcatca attaagtgaa
    13681 tctaaggcac tcagtgaaat tttaagtgag caagcagggc ataaagtcag cattttagat
    13741 aatgccatca gaggtgataa acagcgttat ttagctttag cgagaactaa tgcaatggca
    13801 gcattagttt tacaacttaa gcaagaatcc catattcaaa gccgttatca ggcattgaaa
    13861 gaaatgttga aaatgtcaga gattaaaaga atggagtgct ttgacatcag tcatacaatg
    13921 ggcaaccaaa cagtggcttc ttgcgtggtg tttgatgaaa atgggccgct aaaatcggat
    13981 taccgccgtt ttaacattga ggggattacc ggaggtgatg attatgcagc aatggagcag
    14041 gctctcttga aacgctataa tcgagaatta ccgccagaaa agatccctga tattattttt
    14101 attgatggtg gcaaagggca gttgaaccga gcgttagatg tcttcgctaa acttgatgtg
    14161 gaatgggata aatcgaaacc gcttttaatt ggtgtggcaa aaggcgtaga acgtaaagct
    14221 gggctggaaa ctttaattat cagcaaatgg gataaagaaa ttcacttacc gccagatagt
    14281 cttgccttgc atttaattca gcatattcga gatgaatccc accagcatgc cattagtggg
    14341 catcgtaaaa aacgccaaaa ggcctttacc gaaagtggat tagaatctat tgccggtgtc
    14401 ggtgcaaaac gccgacaagc gttgctaaaa tatttaggcg gaatgcaggg ggtgaagtct
    14461 gcaaccttgg aagagattca atcagttccg ggaatttcta aagcgttggc agaggttatt
    14521 tttgatacat taaaaaatag ttaaattatc aatagcgata tcgcttccag ccgtaatggt
    14581 agcccactcc tatgcttggg taaattatag ggtgaattgg cagatttatg ctttctgagc
    14641 gtttatcgct ttggtttaat ggctcatcat gttgctttgt atttttatca acagtttgct
    14701 tattgattac tcgttgatgg taatcctgaa ctgcttgcat atccattggt actgtgcttt
    14761 gttgttgggg tgtcgaacag gcggttagtg caacgctcaa tccgagtaaa attaattttt
    14821 tcatatcatt tccttatatt gcaaaatatt ttgctttttt gaccgcttat tttgctatta
    14881 attcttaaaa agattatttt atccataaaa aaggacgcac taggcgtcct catttctatt
    14941 ctttaaaatt aaagagcaga ttttgctttt tcaactaaag ctgcgaaagc aactttgtca
    15001 aatactgcga tgtcagctaa gatcttacgg tcgatttcaa cagatgcttt tttcaagccg
    15061 ttgatgaatt tgctgtaaga taaaccgttt tgacgagctg ctgcgttaat acgagcaatc
    15121 cataattgac ggaattgacg tttacgttga cggcggtcac ggtaagcata ttgagcagct
    15181 ttaactactg cttggaaagc aacgcgatac acgcgtgaac gtgcaccata ataaccttta
    15241 gcagccttaa gaactttctt atggcgtgct cttgcaataa caccacgttt tacacgagcc
    15301 attatttaat ctcctaattg tctattttga ttggtggtcg tcatcacgac ctttactaag
    15361 aattctatcc gtacgtccgt actttactac actacgctaa cctaaaaggc ttatgcgtat
    15421 ggtaagcacg ctactactaa aacttggtca gctttcgcta ccattgattt gtgacgtaat
    15481 tgacgtttac gtttagtggt tttcttagtc aaaatgtgac gtaagtgaga ttgtttacgt
    15541 ttaaaaccgc cggaagctgt ttttttgaaa cgcttagcag caccacgtac tgttttaatt
    15601 ttaggcattg ttaaaaaact ccgcattgtt tgttagatat tatgaaaata aggcgaattt
    15661 tgtgaacaaa attacttgga agcacttatt ctccgctata cgattttcat cgttaaaaag
    15721 tcaagcaggc tgctgagttt gcctgttgag cattttctct ttcttggcta ggcaaaaaag
    15781 cttgcgaatt ttagcgaaaa tataaccgct atgcaagcca atttaagctt tatcaagaaa
    15841 aaagcacatt aaaacaatgt gcttatcgaa attatttctt cttaggtgcg atcaccatca
    15901 caacttggcg accttccaat ttgcctgggg ctgactctac cattgcaatt tctgcagtat
    15961 ctgctttgac acgttctaaa acgtccatac cgatttcttg gtgagccatt tcacgaccac
    16021 ggaaacgcac tgtgattttt actttatccc catcttctaa gaaacggata atgctacgaa
    16081 gtttaacttg ataatcgcca tcatcagttc ccggacggaa tttgatctct ttaacttgaa
    16141 caactttttg ttttttcttt tgttcttttg ctgctttctc tttttcgtag atgaacttac
    16201 catagttcat aatacgacaa actggtggtt cagcatttgg gctgatttcc actaaatcca
    16261 attcagcttc ttctgctcta tttagagcat cttgaagtga tacaatacca acttgttcgc
    16321 cgttttcatc ggttaagcga acttctttta cgccacgaat ttcgtcatta agacgatgtg
    16381 cacgatttga ttgaacacgt tttacaggtt taatagcctt attccttcta gtaaattttt
    16441 gcttaaattt taccgcttga gagcgtataa atattttaga ttgcttgttt taagtgatta
    16501 aaaaacagct aaacgtgcgg attctataag attttcagtg cttatgcaat aataaagccc
    16561 ttatttaact gcctattttc ggttattttg atattctttg attaaactca taaatgcttg
    16621 cccaaatcgt tcaaattttc gttcaccaac accattaatt tctaacattt ccacttttga
    16681 agtcgggaga aattctgcca tttcttgcaa ggtggcatcg ttaaagacga tataaggtgg
    16741 aatattttcg aggtcagcta tctgcttgcg taaaaaacgt aagcgtgcga ataggtcttt
    16801 gtcatagcga tgacttgctt gtttttgcac aaatgcagtc gcagatatac ctaaacgtgg
    16861 caatgcaagc tgaagttgct tttctccacg caaaaatggt ctggcacttt cggttaattg
    16921 cagagcggaa tggttagcaa tattttgaat aatcaaccct aaatgaatca gttgccgcac
    16981 cacattttgc caataatgtt ggctttgttc ttttccgata ccataaacgg aaagttgatc
    17041 gtgttgatgt tcacgaattt tgagattgtt cattcctcgc aataccgcaa taatatggtg
    17101 ctgaccgaaa atttgaccgg tgcggtaaat caccgacatc actttttggg catcaagtaa
    17161 gccatcgtat tgttgcggtg gatctaaaca aatatcgcag tttttgcatt gctcttggcg
    17221 atgttcccca aagtagttga gtaagactaa acggcggcaa gtctgtgatt cggcgaaatc
    17281 gccaattgcc tgtaatttat gctgtttaat atttctctgt tcgctttccg gctcttccaa
    17341 taatattttt tgtaaccaag cataatcgct aggatcataa aaaagcacgg cttcagaagg
    17401 gagatcatct cgcccggctc gtccggtttc ttgatagtaa gattcgatag agcgaggtaa
    17461 atcgaaatgt accacaaaac gtacgttgga tttgttgatc cccataccaa acgcaatagt
    17521 ggcaaccaca atttgaatat catcacgctg aaaggcattc tgcactcgtt ctctctgttc
    17581 aaaactcata ccggcgtgat agcccatcac gctgatttta cgggctgaga gtttctcggt
    17641 gatttcttcc acttttttgc gactgttgca gtaaacaata ccgcttttgc cttgctgttt
    17701 gctgatgaat ttaatgagct gttcaagcgg tttaaatttt tcctgcacgg tgtagcgaat
    17761 attggggcga tcaaagctgc ctaaatagat atgtgggcta tttaagcgta aatgatgcag
    17821 aatatcggag cgggttgtta gatctgccgt ggcggtgagt gccatcaacg gcacattcgg
    17881 gaaagtgttt cgtaagccac ctagcagggt atattcaggg cggaaatcgt gcccccattg
    17941 ggaaacacaa tgggcttcat caaccgcaat aaagctgatt ttacatagcg aaataaagtg
    18001 gaaaaagcct tgagtcatta ccttttcagg ggaaagatag agtaatttta attgaccgga
    18061 taaggctttt tgttccacaa gttgctgctc ttcgagcgtt tgagtggagt taagataagc
    18121 tgcttcaatg ccgttggtga taagttgatc cacctggtct ttcatcaacg aaataagcgg
    18181 cgaaatcacc aaagtgatgc cgtctaggca caaagccggc acttggtagc aaagggattt
    18241 gccaccgcct gttgtcataa tcacgaggca atcacgattt tccaacacgg cattgataac
    18301 ttcttcttgc ccgtctcgga atttttgata gccaaaaata ttatttaaaa cagaaattgc
    18361 ggtggtgttc attggtagtt ggttgctact tatgatagta ctcgtcttct gagagtatct
    18421 tttaaaatga aaataaagca ctcatcaaag acgagtgctg tcttagttaa caattaggca
    18481 ttagaaagaa acgctttctc cgtgtcgagc aaaagcttct tctaaatgtt gccagaacga
    18541 gcgaccttca tctgttaccc attcgccttc tacattttta aagtggaaac cacccaattt
    18601 gctggcaagc cataattcca gcattgcctc ttgtttatta atgataactt gcgattcatc
    18661 atcaaaggtt aaggtgcaga ccgctccttg gatttcagta tcgactgata atccttcgtc
    18721 ataaattttt tcttcaattt gttgccatac ttgctctacg gcttggtgga attgtgctgt
    18781 gttcattggt gtatc
//
LOCUS       DANLZC010000037        18357 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.037, whole genome shotgun sequence.
ACCESSION   DANLZC010000037 DANLZC010000000
VERSION     DANLZC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18357
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.037"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            207..1625
                     /gene="leuC"
                     /locus_tag="PXE44_000987"
     CDS             207..1625
                     /gene="leuC"
                     /locus_tag="PXE44_000987"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDL5588512.1"
                     /translation="MQQAKTLYQKLFDSHVVYEAEGETPIIYINRHLIHEVTSPQAFD
                     GLRVAGRQVRQVSKTFGTMDHSISTQVRDVQQLEGQAKIQVLELEKNCEETGIPLFDI
                     TKKQQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     KQARAKSMKVEVRGKVNPGITAKDIVLAIIGKTTMAGGTGYVVEFCGEAIRDLSMEGR
                     MTVCNMAIEFGAKAGIVAPDETTFAYLKDRPNAPKGQDWEDAIEYWKTLKADEGAVYD
                     AEVILEAKDIAPQVTWGTNPGQVIGIDDVIPNPEDMADPVTRASAEKALAYIGLEAHT
                     DMKDVPVDQVFIGSCTNARIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKE
                     GLDKIFIEAGAEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSP
                     AMAAAAAVFGKFMDIRKVSLNA"
     gene            1638..2240
                     /gene="leuD"
                     /locus_tag="PXE44_000988"
     CDS             1638..2240
                     /gene="leuD"
                     /locus_tag="PXE44_000988"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDL5588513.1"
                     /translation="MAGIKQHSGLVVPLDAANVDTDAIIPKQFLQAITRVGFGKHLFH
                     EWRYLDAEETQINPDFVLNFPQYQGATILLARKNLGCGSSREHAPWALADYGFKVMIA
                     PSFADIFYNNSLNNHMLPIKLTEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGEKVYT
                     FELDEFRRHCLLNGLDNIGLTLQHEAAIAAYEKNIPAFLR"
     gene            complement(2312..2776)
                     /locus_tag="PXE44_000989"
     CDS             complement(2312..2776)
                     /locus_tag="PXE44_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588514.1"
                     /translation="MNKFKIALMATVAIGLFACTPSPQSNSTKQQAQVGYLKQNISQA
                     ELNNPKDFKRYSYYCKNFATGSSSYLATYFPLWSESRKQENFGIYFQLDGGKVYPFDH
                     LANISLNARATKFEVRYRSYQPIEGSFVELVAREFTSTYYKNNVPWLECREG"
     gene            complement(2800..3306)
                     /locus_tag="PXE44_000990"
     CDS             complement(2800..3306)
                     /locus_tag="PXE44_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588515.1"
                     /translation="MADPHIQSPMDFWDYLTVIIYRSGFVLATLMLLLLPYYPQTAQY
                     GLLVSGTMLASSLHLYAKSFRLIFQFSVWIGLLGAVFNFPLLAFGAMLLVIGGLSYKE
                     YFCFRVFGLNFQPLLVAVLWIAFALEWSLLVQILSLVCGLLLLVLSIQKWRMPLHFDI
                     GDKSKYQI"
     gene            complement(3329..4306)
                     /gene="dusA"
                     /locus_tag="PXE44_000991"
     CDS             complement(3329..4306)
                     /gene="dusA"
                     /locus_tag="PXE44_000991"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDL5588516.1"
                     /translation="MTNEIQPFYRGRFSVAPMLDWTTRHCRYFHRQFSRQALLYTEMI
                     TAPAIIHAKYDLLEYDPSENPVALQLGGSDPAQLAHCAKLVAERGYAEINLNVGCPSD
                     RVQNGMFGACLMAKADLVADCIKAMQDAVEIPVTVKHRIGIDDLDSYEFLCDFIEKVQ
                     PYSNDFIVHARKAWLTGLSPKENREIPPLDYDRVYQLKRDFPHLKISINGGIKTIEEI
                     KQHLQFVDGVMVGREAYQNPSMLGEIDSQIFNENRPLVTAKEAVEKMFPYIERELAKG
                     VYLNHIVRHMLGAFQHCKGARQWRRHLSENATKSGAGVEVVEQALAFIG"
     gene            4657..5634
                     /locus_tag="PXE44_000992"
     CDS             4657..5634
                     /locus_tag="PXE44_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL5588517.1"
                     /translation="MSRNLFQKRLNIKPYEYPELLEFKDAIRHSYWLHTEFNFTGDIQ
                     DYRTTINDHERHVLTRAMLAISQVEVNVKRFWGELYCYFPKPEMDDVGGTFAESEVRH
                     KDAYSFLLEKLGLNEMFSQIHEIEPLMNRIRYMESFMKDKDAGKGEFVLSLVLFSLFV
                     EHISLFGQFIIMMSFNKHKNLFKGISNAVEATSKEEEIHGKFGIALYEILRDEHSELF
                     TPEFFEDLQTLANQAFEAERGILDWIFEEGDLSFITKATVENYIKNRYNNSLSILGLE
                     PPHDIDPELLKETEWFDIEILSTKETDFFNKRSTDYSKKMKQITADDLF"
     gene            5621..7288
                     /locus_tag="PXE44_000993"
     CDS             5621..7288
                     /locus_tag="PXE44_000993"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL5588518.1"
                     /translation="MTYFNTDRPDFHWLNEDSRLFLQRGYLLEGTTALDRIRFIAEHA
                     ERKLGIEGYADKFYHYMARGYFSLSSPIWSNFGLDRGLPISCFGSYIGDSIHEIMVTT
                     AEVGMMSKIGGGTSAYFGDIRPRGSLIKNNGKSDGSFNFSKLFDTVIDVISQGTSRKG
                     QFAGYIDIEHGDIDEWLDIHTEGNPIQLMYYGVCVGHDWLESMKAGDPYKRQLWAKLL
                     QRKTETGIPYLFFKDNANAGRPDVYKDKNMTIHASNLCTEIMLPSSNEESFVCCLSSM
                     NLLYFDEWKDTDAPETLTYFLDVVMSEFIEKSQSIPFLDRANRFARRHRALGLGVLGW
                     HSYLQANNIAFDSFQAMQKNNEIFKTLQAKTLKASQELAQRFGEPEILEGYGRRNTTL
                     MSIAPTKSSSFILGSVSPSVEPFKSNYYVKDLAKIKTVYKNPFLEKLLQEKGLDTEEI
                     WTSILQNDGSVQHLEQLSEHEKEVFKTFAEISQLSVIQQAAQRQKYIDQGQSINVMIH
                     PATPARDLNQLYLTAEELGLKSIYYQNSMSAAQVFNRNLLSCSSCEG"
     gene            7292..7711
                     /locus_tag="PXE44_000994"
     CDS             7292..7711
                     /locus_tag="PXE44_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588519.1"
                     /translation="MGNLSHTSPNTIVEVVGEFPEITSSGYTFDKGPIQLRIGKHTAP
                     NKGFGVKHIEAEHMQEILNLGFTSVNEFVKAIIVENAKIFCEFSDLKGNHRPIILKAS
                     IGIVVLERKFLESGTIYSVVTAYRRTNPHGIQIGTMK"
     gene            complement(7946..8707)
                     /gene="nudC"
                     /locus_tag="PXE44_000995"
     CDS             complement(7946..8707)
                     /gene="nudC"
                     /locus_tag="PXE44_000995"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDL5588520.1"
                     /translation="MNKGYWIIVSDFDIALEAGEIPFGSAEELGLSGREKLTIGQYQG
                     SPAYLVKALESDNQREFAYLRSQINRPPELSHLLHRAVSLNHFFQTHQFCGKCGEKTI
                     LASDEIAIYCNACGNRAYPTISPSIIVAVRKGRQILLANHLRHKGTIYTTLAGFVEAG
                     EAVETAVHREVFEETGIKVKNVRYFGSQPWAFPNSLMLGFLADYESGEIQLQEEEICD
                     AKWFDCEESLPEIPQQGTIAFELIQATLKLCRESK"
     gene            complement(8700..9383)
                     /locus_tag="PXE44_000996"
     CDS             complement(8700..9383)
                     /locus_tag="PXE44_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL5588521.1"
                     /translation="MKQPTFKRMTNKRSNFAKNLQKNPQKRPLTFAETTLVLLNKPYD
                     VLTQFTDEQGRKTLKDFITMPNIYPVGRLDRDSEGLLLLTNNGEIQHRLAHPKFDKQK
                     TYWVQVEGVPQPEDLAKLAKGVELKDGLTKPAKVRAIATPDFAWNIAPQIRERKTIPT
                     SWIELIISEGKNRQVRRMTAHIGFPTLRLIRVGIGQFRLGNLGCGEFQVLSENEKRKL
                     FTQLGLKDE"
     gene            complement(9380..10228)
                     /locus_tag="PXE44_000997"
     CDS             complement(9380..10228)
                     /locus_tag="PXE44_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5588522.1"
                     /translation="MEKRTLALLKVHGTAVLFGASGIFGALIQSSAETIVLGRVMIAF
                     AIISLYFLWKKQPLVKLNLQQMAQLAFSAALLAAHWVTFFVAVKVGGVAVATLGFASF
                     PAFVALFESLFFREKLRFREVVLLIAITVGLILVTPEFTFGNQATQGLLWGVLSALVY
                     GILAVANRRTMSKISGVQASWWQYIFATLFLLPLSITEFPSVSTQDWFWIFCIGFLCT
                     TLAYTLFISSLDTINARTASMIISLEPVYAIVIAWLCFNEVPTLKMIIGGLIILLSVA
                     WANLKK"
     gene            complement(10228..10713)
                     /locus_tag="PXE44_000998"
     CDS             complement(10228..10713)
                     /locus_tag="PXE44_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HDL5588523.1"
                     /translation="MPILAILFGIFFYIYCEISLLVNIGSIIGVLPTILLLVVISFLG
                     LWIVKLRGVYTLFSIKQDLSQGKIPTEAVGNSVMFIVAGILLIIPGFLSDILAILCVL
                     PFSRKWIQAFVINSVKNKVFMHFTAQTSSFSVTSAQDSNTFDAEFERKPDEQDQHNRI
                     K"
     gene            complement(10715..12037)
                     /gene="pepQ"
                     /locus_tag="PXE44_000999"
     CDS             complement(10715..12037)
                     /gene="pepQ"
                     /locus_tag="PXE44_000999"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDL5588524.1"
                     /translation="MKNLFSTHLARLQQTVQSLLENAFLDGLWIYAGKAKTLFLDDQT
                     APFKINPYFNYFFPYPQAENCWLFLDGKNKPKIYFYAPEDYWVSTLKVPTEAFFAAEF
                     EWVLLRAENEIATFIQNPTACAFIGEDVELAKKLGFSHINPQKVLNSLNFARSIKTEF
                     EIESIYQAQFSALKGHQVAKEAFFAGKSEFEINLEYLKATQQSDLNVPYGNIVAINQN
                     GAVLHYTALDFDTHAKRHSFLIDAGTTVNGYASDLTRTYAFDSGSEFAELVAKMEQLK
                     LQIIAELSIGVNYLHYHTRMQQMISQLLAEHQFIHLSAEQIFEQGISRTFFPHGLGHL
                     LGIQVHDVGGFQQNARGSHKAPPEVYPSLRCTRELQENMVLTIEPGFYFIEMLLKPWK
                     NSALSSAFNWQKIEAFKAFGGIRTEDNIVMRANGAENLTANAAATLGG"
     gene            12048..12290
                     /gene="tusA"
                     /locus_tag="PXE44_001000"
     CDS             12048..12290
                     /gene="tusA"
                     /locus_tag="PXE44_001000"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDL5588525.1"
                     /translation="MTQLTVNKELDTLGLRCPEPVMLTRKTIRNMEEGEILHIIADDP
                     ATTRDIPSFCEFMDHQLIQKEVDSTPYHYWIKKGLE"
     gene            complement(12357..12788)
                     /gene="rraB"
                     /locus_tag="PXE44_001001"
     CDS             complement(12357..12788)
                     /gene="rraB"
                     /locus_tag="PXE44_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDL5588526.1"
                     /translation="MYNLDELRTETDETISSLLADGSDPEALYIIEHHVAHHDFDKLE
                     KLVVDAYKLGYEISEAEEVEDENGKVIFVCDIVSEIHLNADLITAQQKELLPLIEKAG
                     AEYEGWGTYFEDPNADEDEYGDDGEFFDDENDDFEDEPRRH"
     gene            complement(12803..13414)
                     /locus_tag="PXE44_001002"
     CDS             complement(12803..13414)
                     /locus_tag="PXE44_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL5588527.1"
                     /translation="MKLNYHKTHFLTSAPDIRHLPEDTGVEIAFAGRSNAGKSTALNA
                     LTNQKSLARTSKTPGRTQLINLFKVETNCKLVDLPGYGYAAVPEQMKIQWQKALGEYL
                     QKRECLGGVVILMDIRHPLKDLDQQMIEWAVSANLPVLLLLTKADKLSQSARSKQVKM
                     VREAILPFQGDIQVEAYSALNKIGIDKLSNKLDSWFAPKLITE"
     gene            complement(13416..13832)
                     /locus_tag="PXE44_001003"
     CDS             complement(13416..13832)
                     /locus_tag="PXE44_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL5588528.1"
                     /translation="MSWYKALFSFNGRLNRRGFWIGFGIHFLFLFMVAMWINLAELSL
                     LSVLPLIITGFSLSAIIIKRLHDRNRSAKALLMALVPIICYLAAPYSQGIMQILLGQV
                     MPAFIGMLLFLEWGVFAGNPEPNQYGEQGLSFKFRE"
     gene            complement(13836..14666)
                     /locus_tag="PXE44_001004"
     CDS             complement(13836..14666)
                     /locus_tag="PXE44_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL5588529.1"
                     /translation="MFEYFLSLSPIWQAFLAGLFTWGCTIFGSSFVYFFKSVNRKLLD
                     SMMGFAAGVMIAASFWSLLAPSLEYAEADYGSLAWLPAAIGFLAGGAFIRLIDYVVPH
                     LHLSKPIEQAEGNDQLKQGLSKSTLLFLAITIHNIPEGLAIGVAFGALATQVSGVDAS
                     IMGAIGLAIGIGLQNIPEGSSLVLPIRGEGHSRKKAFWYGSMSAVVEPIAAVIGAVFV
                     LSMTAILPYALAFAAGAMIFVVVEELIPESQSSGNTDIATLSLMLGFVVMMVLDVALG
                     "
     gene            complement(15003..15118)
                     /gene="rrf"
                     /locus_tag="PXE44_001005"
     rRNA            complement(15003..15118)
                     /gene="rrf"
                     /locus_tag="PXE44_001005"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(15280..18178)
                     /locus_tag="PXE44_001006"
     rRNA            complement(15280..18178)
                     /locus_tag="PXE44_001006"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         5436 a         4219 c         3549 g         5153 t
ORIGIN      
        1 gaataatatt ctgcacatta ttaacgaata ctatttttag gcttatttag cagaataaaa
       61 acccttattt agcataaaaa tactgtttta ctttcatttt tttaatcgtt atattcactc
      121 catatcagca agcggtcatt tttgcaaaat tttttgcaaa tttaaccgct tgtattaaaa
      181 gttacccttt tgagttggaa cattttatgc aacaagccaa aaccctatat caaaaactat
      241 tcgactcaca cgttgtgtat gaagccgaag gcgaaacgcc gattatttat attaaccgcc
      301 acttaattca cgaagttacc agcccgcaag cgtttgacgg cttgcgtgtt gccggtcgcc
      361 aagttcgcca agtcagtaaa accttcggca caatggacca cagtatttca acccaagtgc
      421 gtgatgtaca gcaacttgaa gggcaagcga aaattcaagt attagaatta gaaaaaaact
      481 gcgaagaaac cggtatccca ctgtttgaca tcactaaaaa acaacaaggt atcgttcacg
      541 tgatggggcc ggagcaaggc ttaactttac ccggtatgac cattgtatgt ggtgactcgc
      601 acaccgccac tcacggggcc tttggtgcgt tagccttcgg tatcggtact tccgaagtgg
      661 aacacgtttt agccacccaa accttaaaac aggcccgtgc aaaaagtatg aaagtggaag
      721 tccgtggcaa agtaaacccg ggcattaccg caaaagatat tgtgcttgcg atcatcggta
      781 aaacaactat ggctggcggt acaggctatg tagtggaatt ttgtggcgaa gcaattcgtg
      841 atctttcaat ggaagggcga atgacagtgt gtaatatggc gattgagttt ggtgcgaaag
      901 ccggtattgt tgccccggat gaaaccacgt tcgcttactt gaaagaccgc ccaaatgcac
      961 caaaaggtca agattgggaa gatgccattg aatattggaa aaccctcaaa gccgatgagg
     1021 gtgcagttta cgatgcggaa gtgatcttag aagcaaaaga cattgctcca caagtgactt
     1081 ggggaaccaa cccggggcag gtgatcggta ttgatgatgt aattccaaat ccggaagata
     1141 tggccgatcc tgttactcgt gcctctgcgg aaaaagcctt agcttacatc ggcttagaag
     1201 cccatacaga tatgaaagat gtaccggttg atcaagtctt tattggttct tgtactaatg
     1261 ctcgtattga agatttgcgt gctgcggcag cagtaatgaa agggcgtaaa aaagccgaca
     1321 atgtaaaacg tattctcgtt gtgccaggtt caggtttggt aaaagagcaa gcagaaaaag
     1381 agggcttgga taaaatcttc attgaagccg gtgcagaatg gcgtaacccg ggttgctcaa
     1441 tgtgtttggg aatgaacgat gaccgcttgg gcgaatggga acgttgtgcc tctacgtcta
     1501 accgtaactt tgagggcaga caaggacgta acggtcgtac ccacttagtc agcccggcaa
     1561 tggcggctgc ggcggcggtg ttcggtaaat ttatggatat tcgcaaagtg tcattaaacg
     1621 cataatagga ggaaaaaatg gcaggtatta aacaacattc aggcttggtc gttcctcttg
     1681 atgcagcgaa cgtagacacc gatgcaatca ttccaaaaca atttttgcaa gccattactc
     1741 gagtcggctt tggtaagcac cttttccacg aatggcgtta tttagatgcg gaagagactc
     1801 aaatcaaccc cgatttcgta ctgaacttcc cacaatatca aggtgcaacc attttactcg
     1861 cccgtaaaaa tttaggctgt ggctcttctc gtgagcacgc cccttgggca ttggcagatt
     1921 acggttttaa agtaatgatc gccccaagtt ttgcggatat tttctacaat aacagcttaa
     1981 ataaccatat gttgccgatt aaattaaccg aagaggaagt cgaagagatc ttccaatggg
     2041 tgtgggcaaa tgaaggaaaa cagatccacg ttgatttaga agcgatgact gtgactgttg
     2101 gcgagaaagt ttataccttt gagttagacg aattccgccg ccactgctta ttaaacggtt
     2161 tggataatat cggcttaacc ctacaacacg aagctgcgat tgctgcttat gagaaaaaca
     2221 ttccggcatt tttacgctaa ttaaaataca aaaaaacggc taacatcttt aaggcgttag
     2281 tcgttttctt tttagccatt ttctttggca attagccttc tcggcactct aaccaaggca
     2341 cattattttt gtaataagtt gaagtaaact ctcgtgccac taactctaca aaactgcctt
     2401 caatcggctg ataagaacga taacgcactt caaattttgt tgctcttgca ttcaaactga
     2461 tgttggcaag atggtcgaac ggataaacct ttccgccatc aagttgaaaa tagataccaa
     2521 aattctcttg cttacggctt tcagaccaaa gtggaaaata ggttgccaaa tatgaactgc
     2581 tgcctgttgc aaaattctta caataatagc tataacgctt aaaatctttc ggattattca
     2641 attccgcttg gctgatattc tgtttcaaat atccaacctg tgcttgttgc ttagttgagt
     2701 ttgattgtgg tgagggcgta caagcaaata agccaattgc cactgttgcc attaaagcaa
     2761 ttttgaattt attcataaaa atcccgataa aatattaaat taaatctgat atttgctttt
     2821 atcgccaata tcaaaatgca gcggcattcg ccatttttga atactcagca caagaagcaa
     2881 caagccacag acaaggctta aaatttgcac caataaagac cattctagtg caaaagcaat
     2941 ccacaatacc gccactaaaa gcggttggaa atttaagccg aatactctaa aacagaaata
     3001 ttctttatag cttaatccgc cgatcaccag tagcattgcc ccgaacgcaa gcaaagggaa
     3061 attaaacaca gcaccaagta agccaatcca aacagaaaat tggaaaatca ggcgaaacga
     3121 tttggcataa agatgcaaag aagatgccaa cattgtgcca gatactaata aaccatattg
     3181 tgccgtttgc ggataataag gcaataaaag caacatcaat gtagctaaca caaaaccgct
     3241 gcggtaaatg atgacggtta aataatccca aaaatccatt ggcgattgaa tatgtggatc
     3301 tgccataact gcctccttaa acaataaatt agccaataaa cgcaagggct tgttccacta
     3361 cttctacacc ggctcccgac ttagtggcat tttcacttaa atgacgacgc cactgcctcg
     3421 ctcctttgca atgttgaaac gcaccaagca tatggcgaac gatatgattt aaatagacac
     3481 ctttggcaag ctcacgttca atataaggga acatcttttc caccgcttct tttgccgtta
     3541 caagcggtcg attttcatta aaaatttgcg aatcgatctc gcctagcatt gacgggtttt
     3601 gataggcctc acgccctacc attaccccat caacaaactg caaatgctgt ttaatttcct
     3661 caatggtttt aatgccgcca ttgatgctaa tctttaaatg cggaaaatct cgcttgagct
     3721 gataaacccg atcataatct aaaggcggaa tttcacggtt ttccttcggg cttaaccctg
     3781 tcagccacgc cttgcgggcg tgtacgataa aatcattgct ataaggttgc actttttcga
     3841 taaaatcaca caaaaattca tagctgtcta aatcatcaat cccaatacga tgtttaaccg
     3901 tgaccggaat ttccaccgca tcttgcattg ctttaataca atccgccact aaatctgctt
     3961 ttgccatcaa acaagcccca aacataccgt tttgcactcg gtctgacggg caaccgacat
     4021 ttagattaat ctcggcatag cctcgctctg ccaccaattt ggcacaatga gccaactgtg
     4081 ccgggtcgct tccacccagt tgcaaagcca ccggattctc gctcggatca tattccaaca
     4141 aatcatattt ggcgtgaata atcgccgggg cggtgatcat ttcggtataa agcaaggctt
     4201 gacgactaaa ttggcgatga aaatagcggc agtgacgggt tgtccaatct aacattggag
     4261 caaccgaaaa acgtccacgg taaaaaggtt gaatttcgtt agtcataaaa atatcaaaat
     4321 aaagcggtaa aaaatcagta aaaaataacc gcttgtaagc gattcaaaac aagcagccat
     4381 catactcgaa aaagccagat aaacatagtt tcctcaaggc ttccccatct atctgcgcac
     4441 ttttaaaaca accttaaaac acaatatata gtgtttcaac atcaaatcac acacaacata
     4501 ttgaagcaaa aatttacaaa attatttttc tttataaatc aataagctac caaagaccaa
     4561 taaaaaagag tgtttgtcaa gacttgtctt gaaacttttt ttgcatacaa tcatctacca
     4621 gttccaaaca taaactttaa gatagagcct acgaatatgt ctagaaacct cttccaaaaa
     4681 cgcttaaata ttaagccgta tgagtatcct gagcttcttg aatttaaaga tgcaattcgc
     4741 cattcttact ggttacacac cgaatttaac ttcaccggcg atattcaaga ctatcgcacc
     4801 accattaacg atcacgaacg tcacgtgtta acccgagcaa tgcttgcgat ttcacaagta
     4861 gaagtgaatg taaaacgttt ctggggcgag ctatactgct atttcccaaa accggagatg
     4921 gacgatgtag gcggtacttt tgctgaaagt gaagttcgcc ataaagatgc ttactcgttc
     4981 ctattggaaa aattaggcct aaatgaaatg ttcagtcaaa ttcatgaaat tgaaccatta
     5041 atgaaccgca ttcgttatat ggaaagtttt atgaaagata aagatgctgg caaaggcgaa
     5101 tttgttttat cgttagtgct gttctcttta tttgttgagc atatttcatt gtttggacag
     5161 tttattatca tgatgtcgtt caacaagcat aaaaacctgt ttaaagggat ttccaatgcg
     5221 gttgaagcca cctcaaaaga ggaagaaatt cacggtaaat tcggaattgc gttatacgaa
     5281 attttacgtg acgaacacag tgaactattc acaccagaat tttttgaaga tttgcaaacc
     5341 ttggcaaatc aagcgtttga agcagaacgt ggcatcttag actggatttt cgaagaaggt
     5401 gatttaagct ttatcaccaa agcgacggta gaaaattaca ttaaaaaccg ttacaacaat
     5461 tctttaagca ttttagggtt agagccgcca cacgacatcg accctgaatt attaaaagag
     5521 actgagtggt ttgatattga aattctttca accaaagaaa ccgatttctt taacaaacgt
     5581 agcaccgatt attctaaaaa aatgaaacag attaccgcag atgacttatt ttaatacaga
     5641 ccgcccggac ttccactggc ttaatgaaga cagccgttta ttcttacaac gtggctattt
     5701 attggaagga acaaccgcat tagaccgcat tcgttttatt gccgaacacg cagagcgaaa
     5761 actcggcatt gaaggctatg ccgataaatt ctatcactat atggcaagag gctacttctc
     5821 gctttcttcg ccaatttggt caaactttgg cttagatcgt ggcttaccga tttcttgttt
     5881 cggtagctac atcggcgact ctatccatga aattatggtg actaccgctg aagtgggcat
     5941 gatgagtaaa attggcggtg gtacttcggc ctactttggc gatattcgtc cgcgtggctc
     6001 gttaattaaa aacaacggta aatcagacgg ctcatttaac ttcagtaaat tatttgacac
     6061 tgtgattgac gtgatttcac aaggaacatc acgcaaaggt cagtttgccg gttatattga
     6121 tattgagcac ggcgatattg acgaatggct ggatattcac accgaaggca acccaattca
     6181 attaatgtat tacggtgtgt gtgtcggtca cgattggtta gagtcaatga aagccggcga
     6241 tccatacaaa cgtcagcttt gggcaaaatt gttacaacgt aaaaccgaaa ccggtatccc
     6301 ttatttattc tttaaagaca atgccaacgc aggtcgtcct gatgtatata aagataaaaa
     6361 tatgacgatt cacgcctcta acttatgtac tgaaattatg ttgccttcca gcaacgaaga
     6421 aagtttcgta tgctgtttat cgtctatgaa cctactctac tttgatgagt ggaaagatac
     6481 cgatgcacca gaaacattaa cctatttcct cgatgttgta atgagtgaat ttattgagaa
     6541 aagtcaatca attccattct tagaccgtgc aaaccgcttt gcccgtcgcc atcgtgcatt
     6601 aggtttaggc gtgttgggct ggcacagcta tctacaggca aataatattg cctttgacag
     6661 cttccaagcg atgcaaaaaa acaacgagat tttcaaaacg cttcaagcaa aaaccttgaa
     6721 agcctcgcaa gaattggctc aacgcttcgg cgagccggaa attttagagg gctatggtcg
     6781 ccgtaatact acattaatga gtattgctcc gactaaatcc agtagcttta ttttaggcag
     6841 tgtgtcgcca tcggtagagc cgtttaaatc caactactat gtaaaagatt tagcgaagat
     6901 caaaaccgtg tataaaaacc cgttcttaga gaaattactc caagaaaaag ggctagacac
     6961 cgaagaaatt tggacgtcta ttttacaaaa tgatggctcg gttcagcatt tggaacagct
     7021 ttccgagcac gaaaaagaag tatttaaaac ctttgccgaa atcagccaat taagcgtgat
     7081 tcagcaagct gctcaacgtc aaaaatatat tgaccaaggg caaagtatta atgtgatgat
     7141 ccacccagcc acaccggctc gtgatcttaa ccaattatac ttaacagcag aagagctggg
     7201 cttaaaatcc atctactacc aaaactcaat gagtgcagca caggtattta accgtaacct
     7261 attaagctgc tcaagttgcg aagggtaaat aatgggaaat ttatcgcata cttcacctaa
     7321 tactattgtt gaagttgttg gtgaatttcc tgaaataaca tctagtgggt atacctttga
     7381 taaaggtcct attcaattaa gaatcggcaa acatactgct ccaaataaag gctttggtgt
     7441 aaagcatatt gaagccgagc atatgcaaga aattcttaat ctaggattta catcagttaa
     7501 tgaatttgtc aaagctatca tagtagaaaa cgctaaaatt ttttgcgaat tctcagattt
     7561 aaaaggcaat catcgcccta ttattctgaa agcaagtatt ggcattgtgg ttctagagcg
     7621 taagtttttg gaaagcggta cgatatattc agttgtaacc gcttatagaa gaacaaatcc
     7681 tcacggaata caaataggca ctatgaaata aagtacgtta gtgttataat cagttccacg
     7741 caagagcatt tgtagaaaat aacaggcgga tttggttttc tactgtgggc ggcaggtttc
     7801 cgctaacctg taatagctat ttaattttta tattaaatcc tgaacttgaa acagactaag
     7861 ggattgctgc ggtagctaag aatacaaacc tctctataat catatagaga ggttttttct
     7921 tattgatgag cacaagttaa gataattatt tactctcccg acataacttc aaggtagctt
     7981 gaatcagctc aaaagctatt gtgccttgtt gtgggatttc aggtaagctt tcctcacagt
     8041 cgaaccattt tgcatcgcag atttcttctt cctgtagctg aatttcaccg ctttcataat
     8101 ccgctaaaaa acctagcatt aatgaattgg gaaacgccca aggttggctt ccaaaataac
     8161 gcacattttt cactttaatg cccgtttctt caaacacttc acgatgcacc gcagtttcta
     8221 ccgcctcacc ggcttcgaca aaaccggcta aagtggtata aattgtgcct ttatggcgta
     8281 gatgattggc tagtaggatt tgtctgcctt ttcgcaccgc cacaataatt gacgggctaa
     8341 tcgttgggta agctcgattg ccacacgcat tgcagtaaat tgcaatttca tcgcttgcca
     8401 gtatggtttt ctcgccacat tttccgcaaa attggtgagt ctgaaagaaa tggttaagcg
     8461 aaacagctcg atgcaacaaa tgagacagtt cgggtggtcg gttaatttgg cttcttaaat
     8521 aagcaaactc tcgctgatta tccgattcta acgccttcac caaataagcc ggcgagcctt
     8581 gatattgccc gatggttaat ttctcacgtc cacttaagcc cagctcttcc gcacttccga
     8641 atggaatttc cccagcctcc aaggcaatat caaaatcgct gacgataatc caatatcctt
     8701 tattcatctt tcaatcctaa ttgtgtaaac aacttacgtt tttcattttc agatagcact
     8761 tgaaactcac cacagcctaa attgcctaat ctaaactggc ctatcccaac acgaattaac
     8821 cgtaaagtcg ggaaaccgat atgtgcagtc attcttctga cttggcgatt tttcccttca
     8881 gaaataatca attctatcca gctggtcgga atggttttcc gctcacgaat ttgcggggcg
     8941 atattccaag caaaatcggg cgttgctatt gctctgactt tcgcgggctt ggtcaaacca
     9001 tcttttaact ccaccccttt tgctaatttg gcaagatctt ccggttgtgg aacaccctcc
     9061 acttgtaccc aataggtttt ctgcttgtca aatttcgggt gagctaaacg gtgctggatt
     9121 tcgccattat tagtcaaaag caacaatcct tcactgtctc tatctagccg cccgaccggg
     9181 taaatattgg gcatcgtaat aaaatctttt agcgtttttc gcccctgttc atcggtaaat
     9241 tgggttagta catcataagg cttattgagt aacacaaggg tggtttccgc aaaggtaagc
     9301 ggtcgttttt gcggattttt ttgcaaattt ttagcgaaat ttgaccgctt gtttgtcatt
     9361 cttttaaagg ttggttgttt cattttttca ggttcgccca cgcaacagaa agtaaaataa
     9421 ttaagccacc gataatcatt ttaagggttg gcacttcatt aaagcaaagc cacgcgatca
     9481 caatcgcata caccggctcg agggaaataa tcattgaggc agtacgggcg ttgatggtat
     9541 caaggcttga aataaacagc gtataggcaa gggttgtaca taaaaaaccg atacagaaaa
     9601 tccaaaacca atcttgggtg gaaacgcttg gaaattcagt gatggagaga ggcaacagaa
     9661 aaagtgtagc aaaaatatac tgccaccagc ttgcttgcac accggaaatt ttgctcatgg
     9721 tacggcgatt cgctaccgcc aaaataccat acaccaaagc agataacacg ccccacaata
     9781 aaccttgagt ggcttgatta ccaaaagtaa attcaggggt aactaaaatt aaaccaaccg
     9841 tgatggcaat taatagcacc acttcacgaa agcgtaattt ttcacggaag aacaggcttt
     9901 caaataacgc cacaaatgcc ggaaagctgg caaagcctaa ggttgctacc gccacaccac
     9961 ctactttaac cgccacaaaa aaagtaaccc agtgggcagc taataaagct gcagaaaatg
    10021 caagttgagc catttgctgt aagttgagct ttacaagcgg ttgttttttc cataaaaaat
    10081 acaaagagat gatagcaaaa gcaatcatca ctcgcccaag cacaatggtt tcagccgagc
    10141 tttgaattaa tgctccgaag atccccgagg caccaaataa aaccgctgtg ccgtgtactt
    10201 taagtagtgc aagtgttctt ttctccacta tttgatccta ttatgttgat cttgctcgtc
    10261 cggcttgcgt tcaaattctg catcaaacgt gttgctatct tgcgcagatg taacagaaaa
    10321 agacgaagtt tgggcggtaa aatgcataaa caccttattc tttacgctat taattacgaa
    10381 ggcttgaatc cattttcggc taaagggtaa cacgcataaa atcgctaaaa tatcacttaa
    10441 aaagccggga ataatcagca aaatgccggc tacaataaac atcaccgagt tgccgactgc
    10501 ttcagtcggt atcttgcctt ggcttaaatc ttgcttgatg gaaaagaggg tataaacgcc
    10561 ccttaacttc acaatccata accctaaaaa tgaaatcacg accagtaaca aaatcgtggg
    10621 taacacgcca atgatcgagc caatattcac cagcagcgaa atttcgcaat agatataaaa
    10681 gaaaatacca aacagaatcg ctaaaatagg catattatcc tcccaacgtt gcggcagcgt
    10741 ttgccgtcag attttctgcc ccattggctc gcatgacaat attgtcttcg gtacgaatcc
    10801 cgccaaaggc tttgaaagcc tcaattttct gccagttaaa ggcagaagat aaggcactgt
    10861 ttttccaagg cttaagtagc atctcaataa aataaaaacc cggctcaatg gtaagcacca
    10921 tattttcctg tagctcacgg gtacaacgca aacttgggta aacctctgga ggagctttat
    10981 ggctgcctcg ggcattttgc tgaaaaccgc ccacatcgtg cacttgaata cccaataaat
    11041 gccctagtcc gtgtgggaag aacgtacggc taatcccttg ttcaaaaatt tgctcggctg
    11101 aaagatgaat aaattgatgt tccgctaata attgcgaaat catctgctgc attcgggtgt
    11161 gataatgcag gtagttcacc ccaatactca gctcagcaat aatctgcaac ttcaactgtt
    11221 ccattttcgc caccagctcg gcaaattcag aaccgctgtc aaaggcatag gtgcgggtga
    11281 gatcggaggc ataaccattt accgttgtgc cggcatcaat taaaaaactg tggcgttttg
    11341 cgtgggtgtc aaaatctaac gccgtataat gtagcaccgc accattttga ttaatcgcca
    11401 caatattgcc ataaggaaca ttgagatcag attgctgcgt cgctttcaaa tattcaaggt
    11461 tgatttcaaa ttcgcttttg ccggcaaaaa atgcctcttt tgctacttga tgccctttta
    11521 aggctgaaaa ttgggcttgg taaatacttt caatttcaaa ctccgttttg atggagcggg
    11581 caaaattcag gctattcagc actttctgcg gattaatatg gctaaagcct aactttttgg
    11641 ctagctcaac atcttcaccg ataaaggcac aagcggtcgg attttggata aaggttgcaa
    11701 tttcattctc tgctctcaac aaaacccact caaattccgc agcgaaaaag gcctccgttg
    11761 gcacttttag agtggaaacc caataatctt caggggcata aaaatagatt ttcggcttat
    11821 ttttgccatc taaaaataac cagcaatttt cagcttgggg atatgggaaa aagtaattaa
    11881 aataagggtt aattttaaac ggggcggttt gatcgtctaa aaataaggtt tttgccttgc
    11941 cggcataaat ccataaaccg tctaaaaagg cattttccag caagctttgc actgtctgtt
    12001 gcaaacgtgc gagatgcgta gagaatagat ttttcataag gaataaaatg actcaattaa
    12061 ctgtaaataa agaattagat accttggggc tacgttgccc agagccggtg atgctcaccc
    12121 gtaaaacaat ccgtaatatg gaagagggag aaattttgca tatcatcgcc gatgacccgg
    12181 ccactacccg tgatattccg agtttctgcg aatttatgga tcaccaatta attcaaaaag
    12241 aggtggactc caccccatac cattactgga ttaaaaaagg cttggaataa accacaagcg
    12301 gtcgatttta gccataaaat tgcaaattta tgcacaaaat cgaccgcttg ttttaattaa
    12361 tgacggcgag gttcgtcctc aaaatcgtca ttttcatcat cgaaaaattc accgtcatcg
    12421 ccgtactcat cttcatcagc attcggatct tcaaagtagg ttccccagcc ctcatactcc
    12481 gcaccggctt tttcaattaa tggcagtaac tctttttgtt gtgcggtaat gagatccgca
    12541 ttcaagtgaa tttcgctcac aatgtcgcat acaaaaatca ctttaccatt ttcgtcttcc
    12601 acttcttccg cttcggaaat ttcatagccc agcttatagg catcaactac taatttttcc
    12661 aatttatcaa aatcgtggtg agcaacgtgg tgttcaataa tataaagggc ttcagggtct
    12721 gaaccatcgg ctaataatga tgaaatggtt tcatcggttt ctgtgcgtaa ttcatcgaga
    12781 ttgtacatat tgaattcctt acttattctg tgattaattt tggtgcaaac cagctatcta
    12841 atttgttcga cagcttatca ataccgattt tattcagtgc ggaataagcc tctacttgga
    12901 tatcaccttg gaatggtaaa atcgcttctc ttaccatttt tacctgctta gagcgtgccg
    12961 attggcttaa cttatccgct ttggtcaaca gcagtaacac cggcaagttg gctgacactg
    13021 cccattcaat catctgttga tctaaatctt tcaacgggtg gcgaatatcc attaaaatca
    13081 ccacaccgcc taaacattca cgcttttgta aatactcgcc taatgctttt tgccactgga
    13141 ttttcatctg ttcaggtact gctgcatagc catagccagg taaatccact aatttacaat
    13201 tcgtttcaac cttaaacaag ttaatcaact gcgttcgccc cggtgttttg gaagttcggg
    13261 ctaaactttt ttggttagtt aaagcgttca aagcagtcga tttaccggca tttgaacgcc
    13321 cggcaaaagc aatctccaca cctgtatctt caggtaaatg acgaatatcc ggtgctgaag
    13381 ttaaaaaatg ggttttatga tagtttaatt tcattttatt ctctaaattt aaaagataag
    13441 ccttgctcgc catattgatt cggttcagga ttaccggcaa atacacccca ttctaaaaag
    13501 agcaacattc caataaatgc cggcatcacc tgtccaagca atatctgcat aattccttgt
    13561 gaataaggag ctgcaagata acaaataatc ggcactaatg ccattaacag ggcttttgcc
    13621 gaacgatttc ggtcgtgtaa tcgtttaata ataatagcag acaggctaaa tcctgtaatt
    13681 ataagcggta gcacactcaa taaagacaat tcagccaaat ttatccacat agcaaccata
    13741 aacaggaaaa gaaagtggat tccaaatcca atccaaaaac ctcgtctatt tagcctaccg
    13801 ttaaaactaa aaagtgcttt ataccaactc attttctacc ctaaagctac atcaagtacc
    13861 atcattacta caaagcctaa catcaggctt aaagtcgcaa tatcagtatt tcccgaactt
    13921 tgcgattccg gaatcagttc ttctacgacc acaaaaatca tcgctccggc agcaaaggca
    13981 agagcataag gtaaaatagc cgtcatcgac agcacaaaaa ccgccccaat caccgccgca
    14041 ataggctcaa ccactgctga catagagcca taccaaaatg ctttcttccg actatgccct
    14101 tcgcctcgaa ttggcaatac aagtgacgag ccttcgggaa tgttttgtaa ccctattccg
    14161 attgccaaac cgatagctcc cataatggaa gcatctacgc ctgaaacttg ggttgccaaa
    14221 gcaccgaaag ccacccctat ggctaatcct tctggaatat tatgaatggt aatcgcaaga
    14281 aaaagcaaag tagattttga taagccttgt ttaagttgat catttccttc agcttgctca
    14341 atcggcttac tcaagtgcaa atgcggcaca acatagtcaa ttaatcgaat aaaagctcca
    14401 ccggcaagaa agccaatcgc tgcaggcagc caagctaaag agccataatc cgcctcggca
    14461 tattctaaag aaggtgcaag caaagaccaa aacgaggctg caatcattac acctgcggca
    14521 aagcccatca tactgtctaa taattttcga ttaaccgatt taaaaaaata gacaaatgac
    14581 gaaccgaaaa tcgtacaacc ccaagtaaat aaacccgcta aaaaagcttg ccatattgga
    14641 gaaagtgaaa ggaaatattc aaacaagaaa tagtaccagc taaaataaat attggctcat
    14701 tctatcaaag attcagggtt attaatatag tgtagccaaa ggctcaacga agatttcact
    14761 tgtaaaactt agcaagaaaa taaccgcttg catttccctt tcataaatac aatgaaaatc
    14821 ctaacaagcg gtcattttcc gtgagttttt tacctttacc acaacaacaa aaaaccgcct
    14881 gtatctctac aggcggctct ttcttggatt acttgtactt tgtttatttg tacttatttt
    14941 gcttttgctt aagttttcgc ttttgtaact tttagctttc gcttaagctc gcttaaatta
    15001 aaacccgacg atgctctact ctcacatggc gaatcaccac actaccatcg acgttactgc
    15061 gttttacttc tgagttcggt atggggtcag gtaggaccac agcactcttg tcgtcgggaa
    15121 aattcttttc tatttttgaa tttctttaag ttaacttcct aatctaactc ttagttcact
    15181 ttaatagtta gattaacttc cttaacttcg attgatttct tctctagttt cgttcttctt
    15241 ctcaatacaa aaacaagcct aaaaacattt gagcgttgta tagttaagcc tctcgggcaa
    15301 ttagtactgg ttagctcaat gtctcacaac acttacacac ccagcctatc tacgtcgtag
    15361 tctccaacaa cccttacaca cttatagtgt gggagaactc atctcgaggc aagtttcgtg
    15421 cttagatgct ttcagcactt atctcttccg catgtagcta cccagcaatg cctctggcga
    15481 gacaactgga acaccagtga tgcgtccact ccggtcctct cgtactagga gcagcccctc
    15541 tcaattctcc aacgcccacg gcagataggg accgaactgt ctcacgacgt tctaaaccca
    15601 gctcgcgtac cactttaaat ggcgaacagc catacccttg ggacctactt cagccccagg
    15661 atgtgatgag ccgacatcga ggtgccaaac accgccgtcg atatgaactc ttgggcggta
    15721 tcagcctgtt atccccggag taccttttat ccgttgagcg atggcccttc cattcagaac
    15781 caccggatca ctatgacctg ctttcgcacc tgctcgactt gtctgtctcg cagttaagct
    15841 tgcttatacc attgcactaa cctcacgatg tccgaccgtg attagcaaac cttcgtgctc
    15901 ctccgttact ctttgggagg agaccgcccc agtcaaacta cccaccagac actgtccgag
    15961 accgcgttcc gcaatcttcg ttagaacatc aaacgttaaa gggtggtatt tcaaggacgc
    16021 ctccacaatc actggcgtga ctgcttcaaa ggctcccacc tatcctacac atcaaaattc
    16081 aatgttcagt gtcaagctat agtaaaggtt cacggggtct ttccgtctag ccgcgggtac
    16141 accgcatctt cacggcgatt tcaatttcac tgagtctcgg gtggagacag cctggccatc
    16201 attatgccat tcgtgcaggt cggaacttac ccgacaagga atttcgctac cttaggaccg
    16261 ttatagttac ggccgccgtt tactggggct tcgatcagga gcttctcttg cgataacacc
    16321 atcaattaac cttccagcac cgggcaggca tcacacccta tacgtccact ttcgtgtttg
    16381 cagagtgctg tgtttttaat aaacagttgc agccagctgg tatcttcgac cggttcaacc
    16441 ttcatccgca agggactaca atctacgccg gcgcaccttc tcccgaagtt acggtgctat
    16501 tttgcctagt tccttcaccc gagttctctc aagcgcctga gtattctcta cctgaccacc
    16561 tgtgtcggtt tatagtacgg tttataataa cctgaagctt agtggctttt cctggaagcg
    16621 tggtatcgat tacttcatct ccgtagagac tcgtcatcac ttctcggtgt taaggaagac
    16681 cggatttgcc taatcttccc acctaccggc ttaaacagac atccaacagt ctgctaacct
    16741 aaccttctcc gtccccacat cgcagttatt acaagtacgg gaatattaac ccgtttccca
    16801 tcgactacgc ttttcagcct cgccttaggg gccgactcac cctgccccga ttaacgttgg
    16861 acaggaaccc ttggtcttcc ggcgaacgag tttttcactc gttttatcgt tacttatgtc
    16921 agcattcgca cttgtgatat ctccagcaga cttctcaatc caccttcatc gacttacaca
    16981 acgctcccct acccaacaga ctttcgtctg atgccgcagc ttcggtgcta tatttgagcc
    17041 ccgttacatc ttccgcgcag gccgactcga ctagtgagct attacgcttt ctttaaatgg
    17101 tggctgcttc taagccaaca tcctagctgt ctaagccttc ccacttcgtt tcccacttaa
    17161 tatagacttt gggaccttag ctggcggtct gggttgtttc cctctccacg atggacgtta
    17221 gcacccaccg tgtgtctcct gagtatcact cttcggtatt cgcagtttgc atcgggttgg
    17281 taatccggga tggaccccta gccgaaacag tgctctaccc ccgaaggtgt ccgctcaagg
    17341 ctctacctaa atagatttcg gggagaacca gctatctccc ggtttgattg gcctttcacc
    17401 cccagccaca agtcatccgc taatttttca acattagtcg gttcggtcct ccagttagtg
    17461 ttacccaacc ttcaacctgc ccatggctag atcaccgggt ttcgggtcta taccttgcaa
    17521 ctacacgccc agttaagact cggtttccct acggctccct tattcagtta accttgctac
    17581 aaaatataag tcgctgaccc attatacaaa aggtacgcag tcacaagatt actcttgctc
    17641 ccactgcttg tacgcacaag gtttcaggtt ctatttcact cccctcgccg gggttctttt
    17701 cacctttcct tcacagtact ggttcactat cggtcaatca ggagtattta gccttggagg
    17761 atggtccccc catcttcaaa caggatatca cgtgtcccgc cctacttgtt gttagcctag
    17821 taccacaatg gagttttaag gtacgggatt atcaccctct acgattgtcc ttcccagaac
    17881 attcccctaa ctttactgct atcactaact ggctctttcg cgttcgctcg ccgctactaa
    17941 cgaaatctcg gttgatttct tttcctcggg gtacttagat gtttcagttc tcccggtttg
    18001 cctcacacag ctatgtattc actgggtgat agtaggttct tcacctactg ggtttcccca
    18061 ttcggacatc ttggattaaa cgcctcttat cgactcatcc aagcttttcg cagattagca
    18121 cgtccttctt cgcctctgat tgccaaggca tccaccttgt gcgcttagtc acttaactat
    18181 acaacctcaa atgttttcta aaacttctca aaaataaaag taaaaaaaca atgaagtcag
    18241 ttttacaact aaacacttga ctgcttttgt tcagtcaaga ttttttctac tcagactttc
    18301 aatttattcg ttatcactta tgtataatta cttatgcgta acgaacttga aatatct
//
LOCUS       DANLZC010000038        18120 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.038, whole genome shotgun sequence.
ACCESSION   DANLZC010000038 DANLZC010000000
VERSION     DANLZC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18120
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.038"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(135..1466)
                     /gene="pcnB"
                     /locus_tag="PXE44_001007"
     CDS             complement(135..1466)
                     /gene="pcnB"
                     /locus_tag="PXE44_001007"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDL5588530.1"
                     /translation="MLHKKFAVNASQYRISPKDFPKNAISIVDKLQIAGYEAYIVGGC
                     IRDLLLEKKPKDFDIATNARPEEIQRIFGRQCRLIGRRFRLAHIVYGREIFEVATFRA
                     GHESHRNDKISKVSDEGMLLRDNVYGTLREDAERRDFTVNALYYDIKRNLIFDFFNGI
                     EDLRAGKLSLIGEPTIRYQEDPVRMLRAVRFMAKLDMFLDKATASPIRDMAVLLTNIP
                     AARLFDESLKLLQTGAGLKTYQLLREYELWQFLFPVLPAFFTAKGDSYAERMITKALN
                     STDERIADKLPVNPAFLFAALLWYPLREKMEQLKNEGGFNSYDAMMLAANDVLAESCN
                     AIALHRRHTSVIRDIWALQFQMMKRSGNRPQQTLEHVKFRAGFDLLVMRAEIEGGDLV
                     ELSAWWHEYQFSNEGQRTELLKSVRHLGNVPSEKKKTRRRRTFKKKPKVNG"
     gene            complement(1711..2157)
                     /gene="dksA"
                     /locus_tag="PXE44_001008"
     CDS             complement(1711..2157)
                     /gene="dksA"
                     /locus_tag="PXE44_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDL5588531.1"
                     /translation="MTQAAKTSLGLLALAGVEPYELKKDEEYMNEAQREHFTKILKAW
                     HSQIMEEAERTKSQLQEDMTNFADPNDRASQEEEFSLELRNRDRERKLLKKIEQTLYK
                     IQEDEYGYCETCGVEIGIKRLEARPTADLCIDCKTLAEIREKQLGL"
     gene            complement(2407..3360)
                     /gene="thiB"
                     /locus_tag="PXE44_001009"
     CDS             complement(2407..3360)
                     /gene="thiB"
                     /locus_tag="PXE44_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDL5588532.1"
                     /translation="MKFILSSLALLSASVYANQPTLTVYTYSSFASEYGPAPKLEQNF
                     EKECGCDVKFVPLDDSVTMFNRLRLEGAKTKADVVVGIDNFLQPEAEKSGLFAEFEAK
                     TKLETQQKIFFPYDTGSYAFIYDKEKLKNPPKSLKELVERQDLKVIYQDPRTSSVGRG
                     FLMWVNQVYGDEAPTAWKTLAKHTVTVGKGWSETYGAFLKGESDLVLSYTTSPLYHKW
                     HENTDKYVAAPFEEGHLVQVENAAIVKSSQQKALANQFLEFLHKPESQHIISYHNVMN
                     PVLTDGVDPAFAAMPTYKTIEFTPPTTETIQKWLADWSKSW"
     regulatory      complement(3404..3547)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3831..4817
                     /locus_tag="PXE44_001010"
     CDS             3831..4817
                     /locus_tag="PXE44_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL5588533.1"
                     /translation="MSQLIRTLKGHIRDEIIKKGGWVNAHAHADRAFTMTPEKITVYQ
                     NANLQQKWDLVDEVKRNSSVEDYYRRFSQAIELMISQGVTAFGTFVDIDQVCEDRAII
                     AAHKAREVYKNDIILKFANQTLKGVIEPNAKKWFDIGSEMVDMIGGLPYRDELDYGKG
                     LEAMDILMDKAKSLGKMLHVHVDQFNSPKEKETEQLCDKSIEHGMQGRVVAIHGISIG
                     SHPKEYRKMLYQKMRDSQMMVIACPMAWIDSGRKEDLQPFHNALTPADELIPEGITVA
                     IGTDNICDYMVPLCEGDMWQELSLLSAGCRFTNLEEMANIASINGRKVLGLV"
     gene            complement(4871..5290)
                     /locus_tag="PXE44_001011"
     CDS             complement(4871..5290)
                     /locus_tag="PXE44_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HDL5588534.1"
                     /translation="MLGNILGSLASSVLSGNNQSTATQLIQNLLASQGGIEGLIAKFQ
                     QGGLDDVLRTWISSDEDNAPVSGEQISNVFGQENIQNVAQEAGVDAADASDLLAQFLP
                     KIVDTLTPNGNVEDLKNFDANDLLAQAAKGILGNLFK"
     gene            complement(5368..6123)
                     /gene="kdsB"
                     /locus_tag="PXE44_001012"
     CDS             complement(5368..6123)
                     /gene="kdsB"
                     /locus_tag="PXE44_001012"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDL5588535.1"
                     /translation="MKFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQLAGASR
                     VIIATDHPEIEALAQSFGAEVCMTSEKHNSGTERLAEVIEKMQIADSEIIVNIQGDEP
                     LIPPVIVTQVAQNLDAYQVNMATLGVKLATREELFNPNAVKVVADKNGMALYFSRASI
                     PFARDSFPDCDDSFVAAQGYLRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALW
                     HGEKIHIEPAKEAPQVGVDTPEDLERVRAILAR"
     gene            complement(6133..7086)
                     /gene="trxB"
                     /locus_tag="PXE44_001013"
     CDS             complement(6133..7086)
                     /gene="trxB"
                     /locus_tag="PXE44_001013"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL5588536.1"
                     /translation="MTTKHTKLLILGSGPAGYTAAVYAARANLNPVLVTGMQQGGQLT
                     TTDEIENWPGEFEQITGTELMNKMLKHAEKFETEIVFDHIHSVDLSKRPFTLNGDLYT
                     FTCDALIIATGASAKYLGLPSEEHFKGKGVSACATCDGFFYRNKPVAVVGGGNTAVEE
                     ALYLANLASEVHLIHRRDSFRAEKILLGRLQKRIEEGKIILHTDRTVDEVLGDNMGVT
                     GLRLKSTKEELSEEIKVDGFFVAIGHAPNTAMFEGQLELENGYIKVKSGLEGNATATS
                     VEGVFAAGDVMDHNYRQAITSAGTGCMAALDAERFLDAQEA"
     gene            7253..8323
                     /gene="dhaK"
                     /locus_tag="PXE44_001014"
     CDS             7253..8323
                     /gene="dhaK"
                     /locus_tag="PXE44_001014"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL5588537.1"
                     /translation="MKKLINSIETVLDEQLSGFVKAHSDLTLNTEPVYIYRTTPSNQP
                     KVALISGGGSGHEPMHAGFIGKGMLDGACPGAIFTSPTPDQMFECGLAVDRGEGVLLI
                     IKNYTGDVLNFETATELLADSGVKVATVLVDDDVAVKDSLYTAGRRGVANTVLIEKLL
                     GAAADKGYNLSELAKLGHQLNNNGHSIGIALSACTVPAAGKPSFTLAENEMEFGVGIH
                     GEPGIERRPFENLDSTVKQMFETLIAHGDYQRKIRHWNTQTQQWDEIEESKVALKKGD
                     RVIALVNNLGAVPLSELYAVYDALEKECQTFGLSIERSLVGSYCTSLDMQGISITLLK
                     VDEDILKLWDAPVNTPAMRWGE"
     gene            8344..8970
                     /gene="dhaL"
                     /locus_tag="PXE44_001015"
     CDS             8344..8970
                     /gene="dhaL"
                     /locus_tag="PXE44_001015"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDL5588538.1"
                     /translation="MTISKQQILKWLENCQQVMTEKRDYLTELDTVIGDGDHGLNMQR
                     GFSKALEKAHTVQDKDIGTLLKTVGMTLLSQVGGASGPLYGSFFIKAAQGATGKEQLS
                     FNELLALLQQGAEAIVQRGHAQVGDKTMCDLWLPLLEDLAQADSTQPIAQILLQAVEK
                     AENFANQTAPLMAKKGRASYLGERSIGHKDPGATSSYYIFKALAEALQ"
     gene            8980..9387
                     /gene="dhaM"
                     /locus_tag="PXE44_001016"
     CDS             8980..9387
                     /gene="dhaM"
                     /locus_tag="PXE44_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL5588539.1"
                     /translation="MVNLVIVSHSAKLAEGVGEILAQMAQGGCKIALAGGIEDTSNPI
                     GTDAVKIMQAIESVFSPDGVIVFVDLGSAILSTQTAIDLLEPELAEKVIISYAPLVEG
                     AFAAVVAASAGDSLEAVLNEANEAAALKQEQAN"
     gene            complement(9427..10272)
                     /locus_tag="PXE44_001017"
     CDS             complement(9427..10272)
                     /locus_tag="PXE44_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HDL5588540.1"
                     /translation="MDYFVNVNEQNFSEVWAAAAQVPTVFHFISPREVASLEMDALLR
                     RISDENPNQFLLATVNCDEQQALAAQFRIQQIPTLYLFAGGQPIDAIQGAVSEQELRI
                     RLANILPKEEELKFQQALELLQDQRYTEALPLLKSAWEMTDKKNSDFALLYAETYIAM
                     KSVEEAREILKAIPIQDRDSRWHGLQAQIELLEQASESPELQQLQADYANSKTPEIAI
                     KLANQLHQAHKNEEALTLLFDWLKSDLNVANGEVKSQFLAILSALGTDPIVPKFRRQL
                     YSLLY"
     gene            complement(10316..10642)
                     /gene="glpE"
                     /locus_tag="PXE44_001018"
     CDS             complement(10316..10642)
                     /gene="glpE"
                     /locus_tag="PXE44_001018"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDL5588541.1"
                     /translation="METTFKEISPQQAWELIENEGAVLADVRDARRYVYSHPQDAFHL
                     TNQSYGKFLDEVDYDEPVVVICYHGVSSQSTAQFLIEQGFENVYSVKGGFEAWERNGL
                     PIETSY"
     gene            10793..11251
                     /gene="exbB"
                     /locus_tag="PXE44_001019"
     CDS             10793..11251
                     /gene="exbB"
                     /locus_tag="PXE44_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HDL5588542.1"
                     /translation="MEQLFQFLEHHTDFVILGLLGLLSVICLWKAIERIMFYSKVDIS
                     KYDTLEELDIDLTHNLTTISTIGSNAPYIGLLGTVVGILLTFYHLGQSGGDIDAASIM
                     VNLSLALKATAVGILVAIIAMVFYNGFGRKVEENKLKWLALQSKKPHQAV"
     gene            11288..11725
                     /gene="exbD"
                     /locus_tag="PXE44_001020"
     CDS             11288..11725
                     /gene="exbD"
                     /locus_tag="PXE44_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDL5588543.1"
                     /translation="MKKFDEINIIPFIDIMLVLLAIVLVTASFISQGKIQVNVPKAST
                     TQPMKADDLAKLLTITENNEFFFNDQAITKEQLIAEVATWDKSQKVSLKVDGAVAFEK
                     FVELTDILSANEIKNVAIITKKETAPAPSSTPGSPAQVPAVAP"
     gene            11741..12652
                     /locus_tag="PXE44_001021"
     CDS             11741..12652
                     /locus_tag="PXE44_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HDL5588544.1"
                     /translation="MKKKHSRIGLLASLAIHTALIGGTFAWLHNHKVEKIAEQNSLSM
                     EMVAALLEQPQVAVAPDPITEEVKEIEPEKAEVPPEPKLTPEPTPEPEAIPDPTLNPK
                     KEKPKEEKRKERHHHKHKHRHKEKREELQEQEKPKDKPKERPKHHHKHIKALERGPEI
                     KQGIVAKAIPNAAEGEKVVAGVVGGQKNGSPNSTSTTGSANTGASSGNASELAAYKVA
                     LQRALQRKANNSYPQREKMMRKTGTVTIKFTITSSGSITNVSVLNSSGNSNLDNAAVR
                     SAEGLSMPPPPAGFPPTVTVPVRFALE"
     gene            complement(12721..13602)
                     /gene="cysZ"
                     /locus_tag="PXE44_001022"
     CDS             complement(12721..13602)
                     /gene="cysZ"
                     /locus_tag="PXE44_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDL5588545.1"
                     /translation="MSSVFSEPQNQVGLGFHYFIYGWRLLLQRQFLPFVIFPILINTV
                     LMVGLIWGFFANIGGLLDMLLPSWLEWLSFVLIPLIFLMILVGFYFAFTTLANFVAAP
                     FNALLAEKVELQLTGEPLGDMSFGAMLKDVPRMLKREWQKMMYSIPRLIALFVLGFLP
                     VVGQTVAPVLTFIFGAWLIAIQYCDYPFDNHKISFQRMRNALSQSRTLNFTFGALVSL
                     FTMIPFVNLVVMPVAVCGATALWVKEYRNFFLNNQTGEFSKADYTFTKVNSTSLSTET
                     RTGEVSPNVRNGDIRKQ"
     gene            13714..14616
                     /gene="zipA"
                     /locus_tag="PXE44_001023"
     CDS             13714..14616
                     /gene="zipA"
                     /locus_tag="PXE44_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDL5588546.1"
                     /translation="MELHIIFFILAGLLIAVLIGFSLWSARREKSNIFSNTFSTRPIA
                     VPPQNNKMSNSIPTSLQPQNEDYQAMSVPEENPQQIQQEVESSLQNIKISLPGGNPQQ
                     QPEQIQPIQSVAEPTVSTYGQKISNQQHIQYNTPDFANPMVNTVVEETPQAVAIEPQE
                     TQSAEEENPFVTLYLYAPQNEAFRGDFVVHYLEEVGLRFGEHQIFHRHQHIDDSLSPI
                     IFSAANLMNPGTFDLNNISNFATAGLVLFMQLPTEGNDVANMKLLISNAEHLAASLGG
                     FLYDDNQQPFGEESRQRYMQRVMH"
     gene            14681..16738
                     /gene="ligA"
                     /locus_tag="PXE44_001024"
     CDS             14681..16738
                     /gene="ligA"
                     /locus_tag="PXE44_001024"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL5588547.1"
                     /translation="MSLFNQTSLFEENATPELVEKIETIRQTLRQYEYEYHVLDNPSV
                     PDAEYDRLMNELKNLEWQHPELISADSPTQRVGAKPLAGFAQVTHEIPMLSLDNAFSD
                     EDLDGFLRRMESYITASPSQLEFCCEPKLDGLAVSILYENGILTRAATRGDGTTGEEI
                     TANIRTIRNIPLKLNTTNPPARLEVRGEVFMPQSGFNELNEKALAKGEKTFANPRNAA
                     AGSLRQLDPKITRQRPLVLNAYGIGVYESDDELPATHFERLQWLKSLGIPVNNEIRLV
                     QGKAALLAYYAEIQAKRPTLGYDIDGTVLKVNDIALQEQLGFISRSSRWAIAYKFPAQ
                     EEMTILKEVDFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLGIVIGDTVI
                     IRRAGDVIPQITGVMLERRPENAKKIEFPTACPICHSAVTRVEGEAVARCTGGLFCEA
                     QRKEALKHFVSRKAMDIDGVGEKLIEQLMARELIETPADLFKLDHTTLMRLERMGEKS
                     AQNALNSIEKAKNTTLPRFLFSLGIREVGEATALNLANHFGSLEAIRQATFEQLKEVQ
                     DVGEVVANRIVSFWQEPHNVEVVEDLIAQGVRWENVVKAEIVDNPLKDKNVVLTGTLT
                     QLTRDEAKALLQSLGCKVSGSVSSKTDYLIAGEKAGSKLAKAQELGIQILSEQQFIEL
                     TQH"
     gene            complement(16801..18069)
                     /locus_tag="PXE44_001025"
     CDS             complement(16801..18069)
                     /locus_tag="PXE44_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL5588548.1"
                     /translation="MTQQIEHSTWQSKFAAMGPGILMASAAVGGSHIIASTQSGAIYG
                     WQLAIIILLANLFKYPFFRFGVQYTLASGKTLLEGYKEKGKFYLIIFFILNVFAAMIN
                     TAAVAIVSAAILKFIFSALGIPLDLSIPMAGTIVIVVTWGILLLGQYKFLDTLSKWIM
                     IALTISTVSAVIIAALKGGVEIAPDFVEPSPWNLAALVFIVALMGWMPAPIEISAINS
                     MWVIAKKRLSKVNYEDGIFDFNVGYIGTAILAIVFLALGALVQYGSGEKVEQAGVAYI
                     AQLIKMYAFAIGDWSKLLIAFIAFMCMFGTTITVIDGYSRANAEALRLISGRKQHSQT
                     ELSIWMTVTALIGILIISQFMSDVAKMLQFAMISSFVSTPVFAWLNFSLVLKGEHRAK
                     GWLFWLAVIGLIYLTSFTLLFLAQQFKLIG"
BASE COUNT         5523 a         3936 c         3623 g         5038 t
ORIGIN      
        1 agcgaaataa gcagagagag gggacaagcg gtaactttta ttaaattttt tacacatttc
       61 ccctctccct gatttttctt ctgaacgaag aaaaatcgct ccctctccca caaggggaga
      121 gggaaatata ttaattatcc atttactttc ggttttttct taaaggttct acggcggcgt
      181 gttttctttt tctcgctagg cacattgcct aaatgacgaa ccgatttcag caattcagta
      241 cgctgacctt cattgctaaa ttggtattcg tgccaccaag cggaaagttc gaccaaatcg
      301 ccgccttcaa tttctgcacg catcactaat aaatcaaacc ctgcacggaa tttgacgtgt
      361 tcaagggttt gttgcggtcg gttgccactt cgtttcatca tctgaaattg caatgcccaa
      421 atatctcgaa tgactgaagt gtgtctgcgg tgcaacgcaa tcgcgttgca actttccgcc
      481 agcacatcat ttgctgccag catcattgca tcgtagctgt tgaagccacc ttcgttttta
      541 agctgctcca ttttctcccg gagcggatac caaagtagtg cagcaaataa aaacgcagga
      601 tttaccggta atttatcggc aattcgctca tcagttgaat ttaacgcttt ggtaatcatt
      661 ctttcggcat aggaatcacc tttggcagta aaaaatgccg gcaataccgg aaacaagaat
      721 tgccatagtt catattcacg caaaagctga taagttttca gccctgcacc ggtttgtaat
      781 aatttgagtg attcgtcaaa caaacgggct gccggaatgt tggttaacaa caccgccata
      841 tcccgaattg gggaggccgt ggctttatcc aagaacatat ccaacttcgc cataaaacga
      901 acggctcgca acattctcac cggatcttct tggtaacgaa tagtaggctc tccgataagc
      961 gaaagtttac ctgctcgtaa gtcttcaata ccgttgaaga aatcaaaaat aagattgcgt
     1021 tttatatcgt aatataaggc gtttacggtg aaatcacggc gctctgcatc ttcacggagc
     1081 gtgccgtaaa cattatcacg cagtagcatt ccttcatcgc tgactttaga aattttgtca
     1141 ttgcgatgag attcgtgtcc tgcacggaaa gtcgccactt caaaaatttc acgcccgtaa
     1201 acaatgtgtg ccaaacggaa acgacgtccg attaatcggc attgcctacc aaaaatacgc
     1261 tgaatttctt caggtctcgc attagtcgca atatcgaaat ctttaggctt tttctccaag
     1321 agtagatctc gaatacagcc acccacaata taagcttcat aaccggcaat ctgcaattta
     1381 tcgacaatcg aaatcgcatt tttaggaaaa tccttcgggc taattctata ttggctggca
     1441 ttcaccgcaa atttcttgtg taacaaatgg gagggaggat tatcttgttg tttcttccca
     1501 cctctataag tcttttggtc cgttaagttc ggggctgggc gtttagaacg ggaagttttg
     1561 ggttctcttt ccgctctttt ttcactctct accttttcat ttttctgagc cgacttttta
     1621 ggcttggaaa ataatgattt aatataatta aaaataatac acctctaaat agtaaatgcc
     1681 tcttaaaaaa gaggcaccct gtaaagttga ttataagcct agctgctttt ctcgaatttc
     1741 agctaaagtt ttacagtcaa tacataaatc agctgttggg cgtgcttcta aacgtttaat
     1801 cccaatttct acgccacagg tttcgcaata accatattca tcttcttgga ttttatacaa
     1861 ggtttgctca atttttttca ataatttgcg ttcacgatca cggttacgaa gctctaaact
     1921 gaactcttct tcttgactgg ctcggtcgtt cggatcggca aagttagtca tatcttcttg
     1981 aagctggctt ttggtacgct ccgcttcttc cataatttga ctgtgccacg ctttcagaat
     2041 tttggtgaaa tgctcacgtt gagcttcatt catatattct tcgtcttttt taagctcata
     2101 aggctcaacg cctgctaatg ctaataaacc gagtgaggtt ttagccgctt gagtcataga
     2161 tatttactcc ttgttccctg tttattggaa gttttattgt taaaattatt gcccgaatac
     2221 agactaactt ccttgtaacc gcactatacg aaaaaatcaa catcttcggg ttttaaataa
     2281 tggcggctat aaataacaca aaaacgactc attaatcaaa tgaatttgca aaaattttgc
     2341 attcaaattg atttgattgc acaaaaaaat agccgaacat ccattcggct atttttcaaa
     2401 cactttttac cagctctttg accaatcggc taaccatttt tggatagttt cagtagtcgg
     2461 tggcgtaaat tcaatggttt tataggttgg cattgccgca aaagccggat caacgccgtc
     2521 ggttaaaacc ggattcatta cgttgtggta agaaataata tgttgcgatt ccggtttgtg
     2581 caaaaattca agaaattgat ttgccaatgc tttttgttgg ctgcttttga caatagccgc
     2641 attttctact tgcaccaaat gcccttcctc aaacggagca gccacatatt tatcggtgtt
     2701 ttcgtgccac ttatgatata aaggcgatgt agtatagctt agtaccaaat ccgactcgcc
     2761 ttttaagaac gctccataag tttcagacca acctttgcct accgttaccg tatgttttgc
     2821 caatgttttc caagcagttg gagcttcatc gccatacact tgatttaccc acattaagaa
     2881 gccccgccca acgctgctgg tacgtgggtc ttggtaaatc acttttaaat cttggcgttc
     2941 caccaactct tttaaacttt ttggtggatt tttcagcttc tctttatcgt aaataaaagc
     3001 ataagaaccc gtgtcataag ggaagaaaat tttctgttgg gtttctaatt tggtctttgc
     3061 ttcaaactcg gcaaacaagc cacttttttc ggcttcaggt tgcaggaaat tatcaatacc
     3121 gaccaccaca tcagctttgg tttttgctcc ctccaaacgc aagcggttga acatcgttac
     3181 gctatcgtca agcggaacaa atttgacatc acagccacac tctttctcaa aattctgctc
     3241 taatttagga gcaggtccat attccgaagc aaacgagctg taagtgtaaa cggttaaggt
     3301 cggttgattg gcataaacac ttgcacttaa taacgcaagt gaagagagga taaatttcat
     3361 aacaattcct tctaaattaa atgaatagaa ggatttaaga aaggcaagtt gctatctcaa
     3421 atccctacgc tgatattaat cagatcaggt tctatgggta tttctcagcc acaagcggtt
     3481 aaatttcggc aaaaatttgc aaatttttac gcaaaaacaa ccgcttgttg caccccaatg
     3541 agaacggctt cattgtaaaa agtttgcagg cagatttcaa ccaaagcaaa cgtttgctat
     3601 ttgtattaaa aatccgtata ctaccgccct agtccaaaca aggaactaga aatgaattgc
     3661 acaacatcgg ttaaatcaaa ttcccaacag ctacgcttca actttagagg cgtagctgtt
     3721 tttctattca tttcaaacca ttcaaaatcg ctgttatagc gatttttttt cgcacgcttg
     3781 caattaccgc aagcggttga gccacattaa cagagagaaa aggaataaaa atgagccaat
     3841 tgattcgtac cctaaaagga catattcgtg atgaaatcat caaaaaaggc ggttgggtaa
     3901 atgcacacgc ccacgcagat cgtgcattta cgatgacgcc tgaaaaaatc accgtttacc
     3961 aaaatgcgaa tctacagcaa aaatgggatt tggtcgatga ggtaaaacgt aattcaagcg
     4021 tagaagatta ctaccgccgc ttttcccaag cgattgaatt gatgatttca caaggggtaa
     4081 cggcatttgg tacttttgtg gatatcgacc aagtatgtga agacagagcc attattgccg
     4141 ctcacaaagc ccgtgaagta tataaaaacg acatcatctt gaaatttgct aaccaaacgt
     4201 tgaaaggcgt gattgaaccc aatgcgaaaa agtggttcga tatcggctcg gaaatggtgg
     4261 atatgattgg tggcttgcct taccgtgacg aactggatta cggcaaaggc ttggaagcaa
     4321 tggatatttt aatggataaa gccaaatcgc tcggcaaaat gctccacgtc cacgttgatc
     4381 aatttaactc acctaaagaa aaagagaccg aacaactgtg tgataaatcg attgagcatg
     4441 gtatgcaagg gcgagtggtt gccattcacg ggatttccat cggctcgcac ccgaaagaat
     4501 atcgaaaaat gctttaccaa aaaatgcgtg atagccaaat gatggtcatt gcctgtccga
     4561 tggcgtggat tgatagcgga cgtaaggaag atttacaacc gttccataac gctttaaccc
     4621 ctgctgatga actcattcct gaaggcatta ctgttgctat cggcacagat aatatctgtg
     4681 attatatggt accgctttgc gagggcgata tgtggcaaga actcagcctt ttatcagcag
     4741 gctgtcgttt tactaaccta gaagaaatgg caaatattgc cagtatcaat ggtcgaaaag
     4801 tactgggttt agtctaactg aataaacgaa aggcgaaccc taggttcgcc tttaataaca
     4861 gcataaccga ttatttaaac aagttaccca aaatcccttt tgccgcttga gctaataagt
     4921 catttgcatc aaagtttttt aagtcttcaa cattaccatt cggggttaaa gtatccacga
     4981 tttttggcaa gaactgggct aataaatcgc tcgcatcagc agcatcaaca cccgcttctt
     5041 gagccacatt ttgaatgttt tcttgcccga acacattact gatttgttcg cccgaaacag
     5101 gtgcattgtc ttcatcgctg ctgatccaag tgcgtaacac atcatctaat ccgccttgtt
     5161 ggaatttggc aatcagccct tcaatcccac cttgtgaggc gagtaaattt tgaattaatt
     5221 gagttgcagt tgattggtta ttgccactca atactgaaga agccaatgaa cctaaaatat
     5281 ttcctaacat aatttctcct atgcaagcgg tacaattttg cgaaaatttt gcaaaaaaac
     5341 agcaaacttc gaccgcttgt aatcacttta acgagctaaa atggctcgca ctctttccaa
     5401 atcttccggc gtatccaccc ccacttgcgg ggcttctttc gccggttcga tatgaatttt
     5461 ttcaccgtgc cataaggctc ttagttgctc aagggactca agctgttcta attgtgtcgg
     5521 ctgccacgcc acatattgtt tcacaaaacc ggctcggtaa gcgtaaatgc cgatatggcg
     5581 taaataacct tgagcggcaa caaaactgtc atcacaatcc ggaaaactgt ctctggcaaa
     5641 cggaatgctg gctcgggaaa aataaagtgc cattccgttt ttgtctgcca ccactttcac
     5701 cgcatttggg ttaaatagct cttctcgggt cgctaatttc acaccgagtg ttgccatatt
     5761 gacttgataa gcatctaaat tttgagctac ctgagtaaca attaccggtg gaatcagcgg
     5821 ttcatcaccc tgaatattca caataatttc gctgtcggca atctgcattt tttcgatcac
     5881 ttccgccaaa cgctcagtgc cggaattgtg tttttccgaa gtcatacaga cttccgcccc
     5941 aaaagattga gcgagggctt caatttcagg gtgatcggtg gcaataatca cacggcttgc
     6001 accggcaagc tgagcttttt cccaaacgtg ttgaatcatc ggtttgcctg caatatccaa
     6061 caacggctta cgaggcaaac ggctggaagc gtaacgagca ggaataatta cagtgaattt
     6121 cataggctat ccttatgctt cttgagcatc taagaaacgt tctgcatcca gggctgccat
     6181 acaacctgtg ccggcagagg taatcgcttg gcgatagtta tgatccatca catcaccggc
     6241 agcaaatacg ccttccacag aagtggcagt agcattgcct tccaaacctg atttcacttt
     6301 aatgtagccg ttttcaagtt ctaattgacc ttcaaacatt gccgtattcg gtgcgtgccc
     6361 aatcgccaca aagaaaccat caaccttaat ttcttcgctc aattcttctt tggttgattt
     6421 taaacgtaag ccggtaacgc ccatattatc gcctaacacc tcatcaaccg tacggtctgt
     6481 gtgcagaata attttgcctt cttcaatacg tttttgcaga cgaccaagca agattttttc
     6541 agcacggaag ctatcacggc ggtgaattaa atgcacttca cttgccaaat ttgccaaata
     6601 aagtgcttct tccacagcag tattcccacc accgactacc gcaaccggtt tattgcgata
     6661 gaagaaacca tcacaagttg cacaagccga cacgcctttg cctttgaaat gctcttcaga
     6721 aggcaaacct aaatatttcg ccgacgcacc ggtggcaata attaacgcat cacaggtgaa
     6781 agtgtataaa tctccgttta gcgtgaatgg gcgtttagat aaatccactg aatgaatatg
     6841 atcaaacacg atttcagttt caaatttttc agcgtgcttg agcattttgt tcattaactc
     6901 tgtgccggta atttgttcaa attcccccgg ccagttctcg atttcatcgg ttgtagtgag
     6961 ctgtccgcct tgttgcatac cggtgactaa aaccgggttt agattggcac gggcggcata
     7021 aacagcagcg gtataaccgg caggccctga gcctaaaatt aaaagtttgg tgtgtttagt
     7081 ggtcataatg aatcctctga ataaatatta atgtttattt tagcctaaat ttcagttcaa
     7141 ctctattttc gttttcgctc aacttttttc aattttgtga caaagttaac agaaatccga
     7201 ctgtgtttct tgtaaattgt gacagtctaa ttcatttaaa aggggcaaaa agatgaaaaa
     7261 attaattaat tcaatagaaa ccgtcttaga tgagcaactt tctggttttg tgaaagcaca
     7321 ttctgattta acacttaaca cagaacctgt ctatatttac cgcacaacgc caagtaatca
     7381 acctaaagta gcccttattt ccggtggtgg gagtgggcac gagccaatgc acgccggttt
     7441 tatcggtaaa ggaatgttag acggagcttg ccccggagca atctttacat ctccaacgcc
     7501 ggatcaaatg tttgaatgcg gtttagcggt tgatcgtggc gagggtgtat tgctaattat
     7561 taaaaactat accggtgatg tgctgaattt tgaaacagcc accgagctac ttgcagatag
     7621 cggtgtaaaa gtcgcaaccg tgttggttga tgatgatgtg gcagtcaaag acagtttata
     7681 tactgccggc cgccgcggtg ttgcaaatac agtattaatt gaaaagctgt taggagctgc
     7741 agcggataaa ggctataact tatccgaatt agccaaatta gggcatcagt taaacaataa
     7801 cgggcattct atcggaattg ccttatctgc ttgtactgtg ccggctgccg gtaagccatc
     7861 atttacttta gctgaaaatg agatggaatt tggggtaggt attcacggag aaccgggcat
     7921 tgaacgtcgc ccctttgaaa atctggatag taccgtgaaa caaatgtttg agaccttaat
     7981 tgctcacggc gattatcaac gtaaaattcg ccattggaat acacaaaccc aacaatggga
     8041 cgaaatcgaa gaaagcaaag tggcattgaa aaaaggtgat cgtgttattg ctttagttaa
     8101 taacttagga gcagtgccac tatccgaact ttatgctgtt tatgatgcgt tagaaaaaga
     8161 gtgccaaact ttcggcttaa gcattgaacg cagtcttgtt ggatcatatt gcacctcgct
     8221 ggacatgcaa ggtatctcta tcacattact gaaagttgat gaggatattc tcaaactatg
     8281 ggatgctccg gtaaatacac cggcaatgcg ttggggtgaa taattagatt gaaaggataa
     8341 gttatgacaa tatcaaaaca acagatctta aaatggcttg aaaattgcca acaggtaatg
     8401 actgaaaaac gggattattt aaccgaattg gataccgtta ttggcgatgg cgatcacggt
     8461 ttaaatatgc agcgtggatt tagtaaagca cttgaaaaag cccataccgt gcaagataaa
     8521 gatatcggca ccctgcttaa aaccgttgga atgacattat tatcgcaagt agggggagcg
     8581 agcggccctc tctatggttc attttttatt aaagcggctc aaggtgctac aggcaaagag
     8641 caactgagct ttaacgagtt actggcactt ctacagcaag gagcagaagc tatcgtacaa
     8701 cgtggacacg cacaagttgg cgataaaacg atgtgtgatt tatggctgcc attactcgaa
     8761 gacttagctc aagccgatag cactcaacca attgctcaaa tcttattaca agcggtcgaa
     8821 aaagcagaaa attttgcaaa tcaaaccgct ccattaatgg cgaaaaaagg gcgtgccagt
     8881 tacttgggcg aacgcagtat cgggcataaa gatccgggag caacgtccag ctactatatt
     8941 tttaaagcct tagctgaagc attacagtag gaggggtaaa tggttaattt agtgattgtt
     9001 tcacatagtg ctaaactggc ggaaggcgtg ggggaaattt tggctcaaat ggcacagggg
     9061 ggctgtaaaa tcgcccttgc cggcggcatt gaggatacct caaatcctat cggcactgat
     9121 gcggtaaaaa taatgcaagc gattgaatcg gtatttagcc cagacggtgt gattgtgttt
     9181 gtcgatttag gcagtgcaat tttaagtacc caaaccgcca tagatttact ggagccggaa
     9241 cttgccgaaa aagtgatcat tagctacgct cctttagttg aaggtgcttt tgctgctgta
     9301 gttgcagcct ctgccggcga tagtttagaa gcggtattaa atgaagcgaa cgaagccgct
     9361 gctcttaagc aggaacaagc caattagtaa aaatgccgta tcgaaaaata cggcatttta
     9421 gttttattag tacaatagtg aataaagctg tcttctaaat ttcggcacaa tcgggtctgt
     9481 acccagtgca gataagattg ccagaaattg gctttttacc tcgccgttcg ctacatttaa
     9541 atccgatttt aaccaatcaa aaagtaacgt gagtgcttct tcatttttat gagcttggtg
     9601 tagctgattt gccaatttaa tcgcaatttc aggggttttg ctgttcgcat aatccgcttg
     9661 aagctgttgt aattcgggcg attcgctggc ttgttccaat aattcgattt gggcttgcaa
     9721 accgtgccaa cggctgtctc tgtcttgaat cgggatcgct ttaaggattt cacgagcctc
     9781 ttccacgctt ttcattgcaa tgtaagtttc ggcatagagc aaagcgaaat cgctgttttt
     9841 cttatcggtc atttcccacg ctgatttcag cagcggcaag gcttcggtat aacgctgatc
     9901 ttgcaataat tccaaggctt gttgaaattt cagctcttcc tctttcggta aaatatttgc
     9961 caaccgaatc cgtaattcct gctcacttac tgcaccttga attgcatcaa tcggctgacc
    10021 gccggcaaac aaatagagcg ttggaatttg ttgaatgcga aattgagcag ccaatgcttg
    10081 ttgctcatca caatttaccg tcgccaataa aaattggtta gggttttcat ccgaaatacg
    10141 acgtagtaag gcatccattt ccaatgaagc cacttccctt ggcgaaataa aatgaaacac
    10201 cgtcggcact tgtgccgctg ctgcccatac ttcactaaaa ttctgctcgt ttacatttac
    10261 aaagtagtcc atacaaaacc ttcttaagct aaaaattact ttttatattg tagcattaat
    10321 aagaagtttc aataggtagc ccgtttctct cccacgcttc aaaaccacct tttacactat
    10381 aaacattctc aaacccttgt tcgattaaaa actgtgctgt actttggcta ctcactccgt
    10441 gatagcaaat cacaaccacc ggttcgtcat aatcgacctc gtctaaaaac ttaccataac
    10501 tctggttggt taaatgaaac gcatcctgtg ggtggctgta aacataacgg cgggcatcac
    10561 gcacatcggc aagcaccgca ccttcgtttt cgattaattc ccaagcctgt tgcgggctaa
    10621 tttctttaaa tgtagtttcc ataatcactt tccctcaaaa aacgtgcaac tataccataa
    10681 aattctaaaa agaatggcta ttattccaag ttgaaaattt gatgtttgtc agatataatt
    10741 gcaaactatt ttcaaatgca ctgatttaaa actaccacag tttgaggatt ttatggagca
    10801 attatttcaa tttttagaac atcatactga ctttgttata ttaggattac taggcttatt
    10861 aagcgtaatc tgcctttgga aagctattga acgtattatg ttttattcca aagtagatat
    10921 ttcaaaatac gataccctcg aagaattaga catcgattta acccacaatt taactaccat
    10981 ttcgactatc ggctctaatg ccccttatat cggcttatta ggaaccgttg tcggtattct
    11041 tttaactttc tatcatttag gtcaatcagg tggtgatatt gatgcggcct ctattatggt
    11101 aaacctttct ttagctctaa aagccactgc tgtcggtatc ttagtggcaa ttattgcaat
    11161 ggttttctac aatggttttg gtcgtaaagt ggaagaaaac aaactgaaat ggttggcatt
    11221 acaatcaaaa aagcctcatc aagcggtgtg atttagtcca ttttttgcaa attaataagg
    11281 cagaacaatg aaaaaattcg atgaaatcaa catcattcca tttattgata ttatgttggt
    11341 attactggct atcgtgttag ttaccgcctc tttcatttct cagggtaaaa tccaagtaaa
    11401 tgtaccgaaa gcaagtacaa cacagccaat gaaagcagac gatttagcga agcttttaac
    11461 cattaccgaa aataatgagt ttttctttaa tgatcaagca ataactaaag agcaattaat
    11521 tgctgaagtt gccacttggg ataaaagcca aaaagtgtca ctcaaagttg atggtgcagt
    11581 cgccttcgaa aaatttgtgg aattaaccga tatcctttct gcaaatgaaa ttaaaaatgt
    11641 agcaatcatt actaaaaaag aaactgctcc agcaccttca agcacacctg gtagccctgc
    11701 acaagtgcca gcagtcgcac cttaaatagg gtaaacaggt atgaaaaaaa aacattctcg
    11761 tatcggctta ttggcatctt tggctattca cactgcctta attggtggca cttttgcttg
    11821 gttacataac cacaaagtgg aaaaaattgc cgagcaaaac tctctttcta tggaaatggt
    11881 ggcagcatta ttagagcaac ctcaggttgc cgttgctccg gatcctatta ccgaagaggt
    11941 caaagaaatc gaaccggaga aggcagaagt accacctgaa cctaagctta ctccggagcc
    12001 aactccagag ccggaggcaa ttccagatcc tacgctaaat ccgaaaaagg aaaaaccgaa
    12061 agaagaaaag cgtaaagagc gacaccacca taaacataag catcgtcaca aagagaaaag
    12121 ggaagagcta caagagcaag aaaaaccgaa agataagcct aaagagcgcc cgaaacatca
    12181 tcacaagcat attaaagcat tagagcgcgg gccagagata aaacaaggta ttgtcgctaa
    12241 agcgattcca aatgcggctg aaggtgaaaa agtggttgcg ggggttgttg gagggcaaaa
    12301 aaacggttca ccaaattcaa cctcaactac aggttcagca aatactgggg ctagcagtgg
    12361 taatgccagt gaacttgctg cttataaagt cgcattacag cgagcattac agcgtaaggc
    12421 gaataactct taccctcagc gtgaaaaaat gatgcggaaa accggtacag ttacgattaa
    12481 atttaccatt acatcgtccg gttcaattac aaatgtatcc gtactcaatt catcgggaaa
    12541 tagtaattta gataacgctg cggtacgcag tgcagaaggt ttgagtatgc ctccgccacc
    12601 ggcaggcttc cctcctacgg taacagtacc ggtaagattt gcccttgaat aaaaacagcc
    12661 ctcaaaagca atgtactttt gagggctttt tattgcaaaa aattgactaa aaatgaccgc
    12721 ttactgcttg cgaatatcgc catttctcac attcggactc acttcacccg tacgagtttc
    12781 tgtgcttaac gaggtagaat ttactttcgt aaaagtataa tccgctttgc tgaattcacc
    12841 tgtttgatta tttaagaaga aattgcggta ctcttttacc cataatgccg ttgcgccaca
    12901 aactgccaca ggcataacta ctaaattcac aaatggaatc atcgtgaata ggctcactaa
    12961 agcaccaaag gtaaaattta aagtgcgaga ctgcgacaac gcatttcgca tacgctgaaa
    13021 actgatttta tggttatcaa acggatagtc acaatattga atggcgatca accaagcacc
    13081 gaagataaaa gtcagcacag gtgctacggt ttgcccaacc acaggcaaaa agcctaatac
    13141 aaaaagagca atcagccttg gaatgctata catcattttt tgccattctc gtttcagcat
    13201 tcgaggcaca tcttttaaca ttgcaccgaa tgacatatcg cccaaaggct cgccggtaag
    13261 ttgcagttcg actttttctg ctaataacgc attaaaggga gcagcaacaa agttagccaa
    13321 ggtagtaaaa gcaaaataaa atcccactaa tatcatcaaa aaaatcagtg gaatcagtac
    13381 aaagctaagc cattccagcc agcttggcag taacatatcc aacaagccac cgatattggc
    13441 aaaaaatccc caaatcagcc ctaccatcag cacggtatta ataagaattg ggaaaatcac
    13501 aaaaggcaaa aattgacgtt gtaacaataa ccgccagcca tatataaaat aatgaaagcc
    13561 taatccgact tgattttgcg gctcagaaaa aactgaagac ataatgattc cttattttct
    13621 atcaaaatta attagaaata tggtaagcta cgcacattat tttcgcaaat atgaaaatac
    13681 attttttatt gattatttga taaagaggac acaatggaac tgcatattat tttttttatt
    13741 cttgccgggc tactgattgc tgtactaatt ggatttagcc tttggtccgc tcgtcgtgaa
    13801 aaatcaaata ttttttcaaa tacattctcg accagaccta ttgctgtgcc accacagaat
    13861 aataaaatga gcaatagcat cccaacctca ttacagccac aaaatgagga ttatcaagca
    13921 atgtctgtgc cggaagaaaa tccgcaacag atacaacagg aagttgaaag ctcgctgcaa
    13981 aatatcaaaa tcagtttgcc gggcggaaat cctcagcaac agcctgagca aattcagccg
    14041 atacaatcgg tagcagaacc aacggttagc acttacggtc agaaaatttc taatcagcaa
    14101 catatccaat acaatacgcc ggattttgca aacccaatgg tgaacacagt ggtggaagaa
    14161 acacctcaag cagtagctat cgaacctcaa gaaacccaat cggcagagga agaaaatcca
    14221 tttgtaaccc tctatcttta cgcaccacaa aatgaagctt tccgcggtga ttttgtagta
    14281 cattatttag aagaagttgg cttacgtttt ggcgaacacc aaattttcca ccgccatcaa
    14341 catattgatg attcattaag tccgattatt ttcagtgcag caaatttaat gaacccagga
    14401 acctttgatt taaataatat ctcaaacttt gcgacagctg gtttagtgct atttatgcaa
    14461 cttccaaccg aaggcaatga tgtggctaat atgaagttgt taatcagtaa cgctgaacac
    14521 ttagcagcat cgttaggggg attcttatat gacgataatc aacagccttt tggcgaagaa
    14581 tcccgccaac gttatatgca acgtgtgatg cactaattgc gaaaaaatta ataaatccga
    14641 ccgcttgtac aagcggtctt ttttgtagag aattttacca atgtctttat ttaaccaaac
    14701 ctctttattt gaagagaatg ccacgcccga gttagtcgag aaaatcgaaa ctattcgcca
    14761 aacattgcgt caatacgagt atgaatatca cgttttagat aacccaagcg tacccgatgc
    14821 cgaatacgac cgtttaatga acgagctgaa aaatctggaa tggcagcacc cggaactgat
    14881 tagtgccgac tcgccgactc aacgggttgg tgccaaaccg ctggcaggct ttgctcaagt
    14941 cacccacgaa atccctatgc tttcgttgga taatgccttc tccgatgaag acttagatgg
    15001 ctttttacgc cgtatggaaa gctatatcac cgcctctcct tcgcaactgg aattttgttg
    15061 cgaacctaaa ttagacggct tggcagtgag tattttatac gaaaacggta tacttacccg
    15121 agctgcaacc cgtggcgatg gcacaaccgg cgaagaaatt accgctaata tccgcaccat
    15181 tcgcaatatt ccgttaaagc tcaacacgac taatccacct gctcgattag aggtgcgagg
    15241 cgaagtcttt atgccacaat caggctttaa tgagctgaat gagaaagcct tagctaaagg
    15301 cgaaaaaacc tttgccaacc ctcgcaatgc cgcagcaggt tcgcttcgcc agttagatcc
    15361 gaaaatcacc cgccagcgtc cgttagtgct gaatgcctac ggaattgggg tgtatgaaag
    15421 cgatgacgaa ctgccagcca cccatttcga acgtttgcaa tggctgaaat cgcttggtat
    15481 tcctgtaaat aatgaaatcc gcttggttca aggcaaagct gctctcttgg cttattatgc
    15541 ggagattcag gctaaacgcc caacattagg ctatgatatt gatggcacag tattgaaagt
    15601 aaatgacatt gccctacaag agcaactcgg gtttatctct cgctcctctc gttgggcgat
    15661 tgcgtataaa tttccggctc aagaagaaat gacaatcctc aaagaagtgg acttccaagt
    15721 gggaagaaca ggggcaatca cccctgtggc gaaattagag cctgtttttg tcgctggcgt
    15781 aacggtaagt aacgcaacgc tacacaacgg tgatgaaatc gagcgacttg gtattgtgat
    15841 tggcgatacg gtaattattc gccgagcggg cgatgtaatt ccgcaaatta caggcgtgat
    15901 gttagaacgc cgcccggaaa atgcaaaaaa aattgaattt ccgaccgctt gtcctatctg
    15961 tcactccgct gttactcggg tagaaggcga ggcagtagca cgctgtaccg gtggtttatt
    16021 ctgcgaagct caacgtaaag aagccttgaa acatttcgtt tcacgcaagg caatggatat
    16081 tgacggcgta ggcgaaaaac taatcgagca gttaatggct cgagagctga ttgaaacgcc
    16141 tgccgatctg tttaagctcg atcacactac cctaatgcga ttagagcgaa tgggggaaaa
    16201 atcggctcaa aatgctctca acagcatcga aaaagcaaaa aacactactc ttcctcgctt
    16261 tttattctcg ttaggtattc gtgaagtggg cgaggcaacc gctctcaact tagccaacca
    16321 ttttggtagc ttagaggcga tccgccaagc cacttttgag caattaaaag aagtgcaaga
    16381 tgtgggcgag gttgttgcca accgtattgt cagcttctgg caagaaccgc ataatgtgga
    16441 agtggtggaa gatttgatcg ctcaaggtgt acgttgggaa aacgttgtga aagccgaaat
    16501 tgtagataac ccgttaaaag ataaaaacgt ggtcttaacc ggcacgctaa cccagctcac
    16561 ccgtgatgaa gccaaagccc tattacaaag tttgggctgt aaagtctccg gctcggtttc
    16621 cagcaaaacc gattacttaa tcgccggcga aaaagccggt tccaagctgg caaaagctca
    16681 agagttggga attcagattt taagcgagca acaatttatt gagcttactc aacattaaca
    16741 aacaaagggg cagatgattg aaatctgccc cttattctat ctattgaatg cttaaaagca
    16801 ttaaccaatc aatttaaact gctgagctaa aaacagtaac gtaaagctag ttaagtaaat
    16861 taagccgata actgccaacc aaaataacca gccttttgca cgatgctcac cttttagcac
    16921 taaagagaaa tttaaccacg caaaaacagg ggtagaaaca aatgatgaaa tcattgcaaa
    16981 ttgtaacatt ttcgccacat cactcataaa ttggctaata attaaaatgc cgatgagtgc
    17041 agttaccgtc atccaaatac ttaattctgt ttgtgaatgt tgcttacgcc cgctaattaa
    17101 acgcaacgct tctgcattcg ctcgtgaata tccatcaata acagtgatcg ttgtgccgaa
    17161 catacacata aatgcaataa atgcgattaa aagcttagac cagtcaccaa tcgcaaaagc
    17221 atacattttg attaattgag caatataggc aacaccggct tgttctactt tctcacctga
    17281 accgtactga actaacgcac ctaatgctaa aaaaacaatc gccaaaattg ccgtgccgat
    17341 ataacccaca ttaaaatcaa aaataccgtc ctcataattc actttactta aacgtttttt
    17401 cgcaataacc cacattgagt taatggcaga aatttcaatt ggtgcaggca tccagcccat
    17461 taatgcaacg atgaacacaa gtgctgctaa attccacggc gaaggctcta cgaaatctgg
    17521 agcaatttca acgccacctt ttaaagcagc aatgatcacg gcagataccg tagagatggt
    17581 taaagcaatc ataatccatt tagataatgt atctaagaat ttatattgcc ctagaagtaa
    17641 aataccccaa gtgaccacaa tcacaatagt tcctgccatt ggaatgctta aatctaacgg
    17701 aatacctaaa gcagaaaaaa taaattttag aatcgctgct gaaacaatcg ccaccgcagc
    17761 tgtgttaatc attgcagcaa aaacgttaag gataaagaaa ataataagat aaaactttcc
    17821 tttttctttg tagccttcca ataaagtttt accgctagct aaggtatatt gcacaccaaa
    17881 gcggaaaaac ggatatttaa ataaattggc taacagaata ataatcgcca gctgccagcc
    17941 ataaatcgca ccagattgtg tagaggcaat aatatgcgaa ccacccactg ccgcagatgc
    18001 catcaaaatc cccggcccca ttgcagcaaa tttactttgc caagttgaat gttctatttg
    18061 ttgtgtcata tagctttcct tatcacatta ctatcaaaac aagatggttt cattctactt
//
LOCUS       DANLZC010000039        18095 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.039, whole genome shotgun sequence.
ACCESSION   DANLZC010000039 DANLZC010000000
VERSION     DANLZC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18095
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.039"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(47..4033)
                     /gene="hrpA"
                     /locus_tag="PXE44_001026"
     CDS             complement(47..4033)
                     /gene="hrpA"
                     /locus_tag="PXE44_001026"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDL5588549.1"
                     /translation="METPLQKNHKKPPLVKLENITTADFRRLLGRLKGAENIKNIDHK
                     RSVLAEISQDLTACQARFFARTQHAQNLKIDYPDLPVSARREEILKLISENQVVVIAG
                     ETGSGKTTQLPKMCLELGRGVKGLIGHTQPRRIAARSVATRIAEELKSELGETVGYKV
                     RFNDQVSENSLIKLMTDGILLAEIQHDSYLNQYDTLIIDEAHERSLNNDFILGYLKQI
                     LHKRPDLKVIITSATIDVERFSKHFNNAPIIEVSGRTFPVEVRYRPIVEDQDQLQGIL
                     NAVDELQAEGRGDILIFMNGEREIRDTAEALQKQELRHSEILPLYARLSAAEQQRIFQ
                     PSGGLNRIILATNVAETSLTIPNIKYVIDTGTARISRYSYRTKVQRLPIEPISQASAN
                     QRKGRCGRTSEGICIRLYSEEDFNSRPQFTDPEILRTNLAAVILQMTSLGLSDIANFP
                     FVDAPDSRQIQDGIRLLEELQAIIPNEKIPSTACGGGLGRGEIRSNDGTPPLPTSPRK
                     RGDGFRLTPIGRQLAQLPVDPRLGRMVIEAAKNGSLHEVMMIVSALSIQDPRERPQEK
                     QQSADDKHRRFADKESDFLAFVNLWHFIQAQQKELSKNQFRNLCRKDYLNYNRVREWQ
                     DIYHQLRLAVREMGLKINSEPASYQPIHTALLAGLLSHIGLKDNEKMHYLGARNAHFF
                     IFPNSALFKKQPKWVMAAELVETTKLWGRMVARIEPEWVEPLAVHLTKSSYSEPHWAK
                     SKGAVMASEKVSLYGIPIVAQRSVNYGSIDPTVSREIFIRSALVEGECHNNYKFFKEN
                     HRLIREVEALEHKSRRRDILVDEQVLFEFYDQRIGTEVVSSKHFDTWWKQASKKDPEL
                     LNFEKSFLINDNANLASELDFPNFWHQGDLKLKLSYQFEVGKDHDGVTVHIPLPLLNQ
                     VEPEGFDWQIPGLRHELIVSLIKSLPKSLRRNFVPAPNYADAFLGRAEPFKKPLLDNL
                     SHELRRMTGVLVEPEQWDLSQLPPHLRMTFRVIDDKGKKLQESENLDELKFALKDEVQ
                     NTLSNIADDGIEQSGVHIWNFAELPQFYEQKKAHFSVKAYPAIVDEQTAVGVKLFETE
                     FEQARAMQAGLRRLLLLNVPSPIKYLHEKLPNKAKLGLYFAPFGKVLELIDDCIACAV
                     DQLVEEFGGFVWTEEKFTQLHEFVRANLNDKTVEIAKQVEKCLTLAYELNKRLKGKLD
                     FTMAFALSDIKTQIEGLIYPDFVTKTGYARLADLYRYLTAIERRLDKLLVDSNTDRAK
                     MLRVEAVQEAYKQLLAKLPKSKAVPDEVLEIRYMIEELRVSLFAQQLGTKYPISDKRI
                     LNVINEIK"
     gene            complement(4137..4589)
                     /locus_tag="PXE44_001027"
     CDS             complement(4137..4589)
                     /locus_tag="PXE44_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL5588550.1"
                     /translation="MTLQFNAVALLLVVLIILGLISQNSAVTISAAVLLIMQQTLLSK
                     FIPFVDQYGLKIGIIILTIGVLSPLVSGRITLPELAQLLNWKMALSIIAGVLVAWLGG
                     RGVNLMGSQPVLVTGLLIGTVIGVAFLKGVPVGPLIAAGILSLIVGKT"
     gene            complement(4652..4921)
                     /locus_tag="PXE44_001028"
     CDS             complement(4652..4921)
                     /locus_tag="PXE44_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588551.1"
                     /translation="MDAAMSLRFDKLRFVKRLQEANQTPEMAEAFANALDEALEQSQS
                     PLATKADLQLELEKLKNEINTTIFKAITLNITILGFLMAIMKFIN"
     gene            complement(5019..5615)
                     /gene="grpE"
                     /locus_tag="PXE44_001029"
     CDS             complement(5019..5615)
                     /gene="grpE"
                     /locus_tag="PXE44_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL5588552.1"
                     /translation="MTTQNEHLQEEVKQEEIQPEVVTEETQAEEQNTPQEEPLALAQA
                     RIAELEAYIAEADKREQDIQLRAQAEIQNIRRRAEQDVEKAHKFALEKFSKELLTVVD
                     NLERGLDALDKAVTDETTQALVDGVEMTHKEFINTLAKFGVVAIGEVGEAFNPELHEA
                     ISMQPAEGIEANHVSTVLQKGYTLHGRVIRPAMVMVAN"
     gene            5837..6931
                     /locus_tag="PXE44_001030"
     CDS             5837..6931
                     /locus_tag="PXE44_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL5588553.1"
                     /translation="MKKLAGLFAAGLATVALTACNEEKAAEPKATAAEAKPQATNQTV
                     HLYTWTEYVPEGLLDEFTKETGIKVEVSSLESNETMYAKLKLQGKDGGYDVIAPSNYF
                     VSKMAKEGMLAELDHAQLPVIKELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNTADYK
                     GETFTSWGDLWKPEFAGKVQLLDDAREVFNIALLKLGKNPNTTNPDEIKEAFEELKKL
                     RPNVLSFTSDNPANSFIAGEVSVGQLWNGSVRIAKKENAPVDMVFPKEGPVLWVDTLA
                     IPANAKNKENAHKLINYLLSAKVAEKLTLAIGYPTSNVEALKVLPKEITEDTAIYPSA
                     EVLQKSQWQDDVGDAIELYEKYYQELKAAK"
     gene            7067..8458
                     /locus_tag="PXE44_001031"
     CDS             7067..8458
                     /locus_tag="PXE44_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL5588554.1"
                     /translation="MNLRWQEYPENARKLFKLSLPIFISQLSASGMGLADIVMAGLVS
                     DDDVSAIAVSNSIYFPLFLFVSGLLNAITPTVSYLNGSSNRHLIANQVRQGYWIVFAM
                     SIPLMAIFLNSHLILDLMQTPAEFSIKSQQYLAVMAVGLIPALLAVSLRCMNDGLSNP
                     KPAMRITFFGLLLNIPLNYIFIFGKFGLPEMGAVGCGVATAIVNLIMFFMLLHYCYTN
                     SAQKDIRLFEKWIDAPSGQILLKLCKLGLPIAFAIFTEVMLFSTSSLFLSPLGSQVVA
                     SHQAALQTSSLFFMIPMSFGIATTIMIGQTLGQKNVEGAKILSYHALITATALAIIAA
                     IIIILLKNFIPFAFTNNPISIAITAHLLIFAAVYQIPDAIQAVANGILRGYKHTQPIL
                     YVTLFCYWGVSMPLGYILARTDWIVQPMAAQGFWLVFCIGLSLAGGLLLWQMRKIQAV
                     PKAQLIAKLERIK"
     gene            8517..12041
                     /gene="recB"
                     /locus_tag="PXE44_001032"
     CDS             8517..12041
                     /gene="recB"
                     /locus_tag="PXE44_001032"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDL5588555.1"
                     /translation="MKNLNPIQLPLNKSVLIEASAGTGKTFTIANLYLRLLLGIGCNP
                     LTVEQILVVTFTKAATEELKNRIRKNIQQCADFLKDQADGLEVESTKSYRNNLDFLAQ
                     IYPLIPNIHEALLRLSIAEREIDTASVFTIHGFCQKMLVQFAFESGVRFDLDLQPNQS
                     DLLKKLSEEVWREQFYPQDLAITYAVAEQLGTPEYALNAVRRYLSTELPEPNASLNQD
                     IAEHLAQYQQFINEVKSYWKTHSDELVETIRHDLKHKITLHGGWYRATTFEAGVEVMN
                     QWANSQHNHFPAGLYLFTTEKIAAGTKKSCEPIDSPHLAKNQAFLTAYKERFENSLSA
                     MLQYQFLIALKAKLAEYKQSHPQRSFDDLLTMLNQALKNLHTGEALSQKIRAMYPFAM
                     IDEFQDTDLTQYEIFSQIFMAENAENGFIMIGDPKQSIYKFRGADIFTYLRAAKQADE
                     QFTLGKNWRSLPEVVQAVNNLFSFANDNSPFLYEDIGFHPVEADSQKGQLVSEQGHFV
                     GYITENSKLDDLAEMCAYQIHQQLEAMAQGKFGVEKNGEFQSFAAKDIAILVRKGSQA
                     KRIKKALAKRNIKSVYLAESASVFGSEIATELCWLLQAALNPYHYKALLSALGSTLWG
                     LTAAEIHRYKENEQLWDLKVNQFVEYSNIWKEQGVLPMLHKIYLEQGIIERLKGLENA
                     DRLITDLLHLTEILQETSQALESEAELLRWYEKQVNSPDFNDEQQLRLESEEELIKIV
                     TIHGSKGLQYPIVWLPFVSQKSEGDTQTALSLYRNSQGALDWCLGSEEASFSLQYEAY
                     AEDLRLLYVALTRAESQLNLVLPKEISGWSPLHYLLADGEIPTGKVPVSDYFSQKRIA
                     ANLVEVAGELPFSLWEGEQSSDDYQAKTFSGNIRVVGQITSFTSLYAQHERFSEGKSE
                     RVMDLAQDRDVHIETIEAGYSSETAAFSPFQFPHGIKVGTVLHHFFEHCQFNEQIDRE
                     AVAKVCEQLGLSEEWIEPTALWFERILTTPLAEANFCLKAIDETKRLNEWQFYLRLKN
                     DKALHQLNALLKQHSPLAKTLPELQLPQLEGFVRGFVDCIVQVEEKFYLIDYKSNFLG
                     YLPQNYAKEHIQREMGRQRYDLQYLLYTLALHRYLTARLGEKYDYERDFGGVAYLFLR
                     AMNGENDNGVYFDKPTKQLIEEMDRLFG"
     gene            complement(12083..12565)
                     /gene="greB"
                     /locus_tag="PXE44_001033"
     CDS             complement(12083..12565)
                     /gene="greB"
                     /locus_tag="PXE44_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDL5588556.1"
                     /translation="MAKSNYITRSGWQALDQELKFLWREERPKVTQAVSDAAALGDRS
                     ENAEYIYGKRRLREIDRRVRFLSKRLEVLQIVDYHPKQEGKVFFGAWVELENEEGEAK
                     QYRIVGCDEFDPAKNWISIDSPVARALIGKQVDDEIRVETPLGKVLLYINKIWYEQAS
                     "
     gene            complement(12616..13896)
                     /gene="pepB"
                     /locus_tag="PXE44_001034"
     CDS             complement(12616..13896)
                     /gene="pepB"
                     /locus_tag="PXE44_001034"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDL5588557.1"
                     /translation="MTAMKISLSTQNAPEIWGKNAILSANDAGFTVHNPNNDLATIQK
                     AARKIKNQGISTVELTGDSWDLESCWAFHQGFHSVKNQGSVQYPDLDEQQAEFDNRVK
                     CATFVRELINKPSDKLTPEKLAEQAVEFIQKMAKPNTVSYQIIRGEALQEQGYHGIWT
                     VGKGSANPPAFLVLDFNPTNDENAQVLACLVGKGITFDTGGYSLKPSDSMSTMRTDMG
                     GAALLTGSLAFAIARGLNKRVKLYLCCAENMVSHNAFKLGDIITYRNGVTVEVLNTDA
                     EGRLVLADGLIDACKQNPQFIIDAATLTGAAKVAVGNDYHSVLSMDDNLTEALFKSAK
                     TENEPFWRLPFEEFHRGQIASSFADIANIGSVPVGAGASTATAFLSYFVENYQQNWLH
                     IDASATFRKTASDLWAAGATGLGIRTVANLLLSK"
     gene            complement(14035..14856)
                     /locus_tag="PXE44_001035"
     CDS             complement(14035..14856)
                     /locus_tag="PXE44_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDL5588558.1"
                     /translation="MKNIKLSAMSLTALFVLTACSHTHKNSDEHHQMVLQEQATLGIN
                     WMQQSGEYQALAHQAFNAAKVAFDQAKVKKGKKKAVVVDLDETMVDNSLYAGWQVKNH
                     KPFDGESWTRWVNARQTGAIAGAVEFNNYVNSHKGTMFYVSNRKDSSEKAGTIDDMNK
                     LGFSGVSEQTLLLKKDKSNKTPRFEEIEKQGYEIVLYIGDNLNDFGDATYRKSNAERR
                     AFVAENSQQFGKKFIMLPNPNYGDWEGGLDKDYYKGNAESRVKIRHNAIKAWDGK"
     gene            15004..15947
                     /locus_tag="PXE44_001036"
                     /pseudo
     CDS             15004..15947
                     /locus_tag="PXE44_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(16060..17223)
                     /locus_tag="PXE44_001037"
     CDS             complement(16060..17223)
                     /locus_tag="PXE44_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL5588559.1"
                     /translation="MKRKTLATLVLIAALGIGGYFYYQQQTNKPQIHYITQAVTRAEI
                     NKNVLATGSVRANKRTEVGAQVSGKIQKLYVELGQSVKQGDLIADIDSSNQSNTLSTA
                     QARLSSYKAQLTSAQVALEVAQSNYNRLNKLYRANSASLNDLETAKNTLASAKATVDN
                     IKAQLKSAEISVNDAKTNLTYTQIISPMDGVIVSVPVSVGQTVNSNQTSPTIVQVADL
                     SKMLIKLEISEGDIAQVKVGQKVKFSTLAEPNREYSATIDSVDPALTTLTDNNYTEQS
                     GNTEAVYYYANVSVDNADNSLRIGMTTQAQITIDKREQVLVVPTSVIKKRGKENYVLV
                     LENEQSVERKIQIGISDTQNTEVLAGLNEGDQVIVTQRADDEKISTARGPRMF"
     gene            complement(17289..>18095)
                     /locus_tag="PXE44_001038"
     CDS             complement(17289..>18095)
                     /locus_tag="PXE44_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL5588560.1"
                     /translation="PFRAIISDLDGTLLNANHKIGDFTIDTLEQLSKKGVDIFFATGR
                     NHPDVSQIIGKVNVKNAMLVTSNGARANNLEGKKILNHYLPEDLAFELMNIEFDDQNV
                     CLNSYQGDEWFINKEIAELKKFHQDSGFSYQVVNFAHHHGRNTEKVFFIGKTAEALVP
                     IEHYIRDTYGDRVQMTYSALLCLEVMAKGVCKANALAELVKLRGYTLADCIAFGDGMN
                     DVEMLSLAGKGCLMGNADPRLKAVLPNNEVIGFNKDEAVASYLRATFGIV"
BASE COUNT         4886 a         3792 c         3931 g         5486 t
ORIGIN      
        1 gttgcaaaat tttttactta tctgaccgct tgttgtatag ggaatattac tttatttcat
       61 tgatcacatt caaaatccgc ttatccgaaa tcgggtattt tgtgccgagc tgttgagcga
      121 acagactgac ccgcagttct tcgatcatat atcgaatttc cagtacctcg tccggcaccg
      181 ctttggattt tggcagtttg gcaaggagct gtttatatgc ctcttgcact gcttccaccc
      241 gtagcatttt ggctcggtcg gtgttgctgt ccaccaacag tttatccaaa cggcgttcga
      301 tagcggtcaa ataacggtat aaatctgcaa ggcgggcgta gccggttttg gtgacaaagt
      361 cggggtaaat cagcccttcg atttgggttt tgatgtcgct taacgcaaac gccatcgtaa
      421 aatcgagctt gccttttaac cgcttgttta gctcgtaggc cagtgtgagg cacttttcaa
      481 cctgtttggc aatttctacg gttttatcgt ttaaatttgc ccgcacaaat tcgtgcagtt
      541 gggtgaattt ttcctccgtc cagacaaacc cgccgaactc ttctaccagc tgatctaccg
      601 cacaggcaat gcagtcgtca atcagctcca atactttgcc gaacggggca aaatataagc
      661 ccagcttggc tttgttcggc agtttttcgt gtagatattt aatcggcgat ggcacattta
      721 gcaacagcag acggcgaagt cctgcctgca ttgcacgagc ctgctcaaat tccgtttcaa
      781 acagtttcac ccccacagcc gtttgttcat cgacaatcgc agggtaggct ttcacgctga
      841 aatgggcttt tttctgctca tagaactgtg gcaattcggc aaaattccaa atatgcacac
      901 cactttgctc aatgccgtca tcggcaatgt tggaaagggt gttctgcacc tcatctttca
      961 aggcaaattt cagctcatca aggttttcgc tctcttgcag tttcttgcct ttatcatcaa
     1021 tcacacggaa agtcatccgc aaatgtggcg gcagttggct taaatcccac tgttcaggct
     1081 cgaccaatac acccgtcatt cggcgtagtt cgtggcttag attgtccagc aacggttttt
     1141 taaacggctc ggctcgccct aaaaaggcgt ccgcataatt cggcgccggc acaaaatttc
     1201 gccgtagcga tttcggtaaa gatttaatca gcgagacgat cagctcgtgg cgtagccccg
     1261 gaatttgcca atcgaaacct tcgggttcca cttggttaag cagcggtagc ggaatatgca
     1321 ccgtcacgcc atcgtgatct tttccgacct caaactgata gctcagtttg agttttaaat
     1381 cgccttgatg ccagaaattc gggaaatcca gctcgctggc aaggttggcg ttgtcgttga
     1441 tcaggaacga tttttcaaag ttcagcagtt caggatcttt tttgcttgcc tgtttccacc
     1501 aagtatcaaa atgcttgctc gacacaactt cggtgccaat ccgctgatcg taaaactcaa
     1561 acagcacctg ctcgtccacc aaaatatcac ggcggcggga tttgtgttcc aatgcctcaa
     1621 cttcacggat caagcggtga ttttctttga aaaatttgta attgttgtga cattcgcctt
     1681 ccaccaacgc cgagcggata aaaatctcac ggctgacggt tgggtctatc gagccataat
     1741 tcaccgagcg ttgtgccaca atcggaatgc cgtagagcga cactttttcc gatgccatta
     1801 ccgctccttt tgacttcgcc cagtgtggtt cgctgtaact ggatttagtg aggtgtactg
     1861 ccaacggttc cacccattcc ggctcaatgc gagcgaccat tcgtccccaa agtttggtag
     1921 tttccaccag ctctgcggcc attacccatt tcggctgttt tttgaaaagt gccgagttcg
     1981 ggaaaatgaa aaagtgggca tttcttgctc ctaaatagtg cattttttca ttatctttca
     2041 agccgatatg cgacaacaag ccggcgagca gggcggtgtg aatcggctga taacttgccg
     2101 gctcactgtt aatttttaag cccatttctc taactgcaag gcggagttgg tggtagatat
     2161 cctgccactc ccgcacacgg ttgtagttca aataatcctt acggcagaga ttacggaact
     2221 ggtttttcga cagttctttt tgttgggctt ggataaagtg ccacaaattc acaaaggcta
     2281 agaaatcgct ctctttatcg gcaaaacggc ggtgtttatc gtctgccgac tgctgttttt
     2341 cctgcggacg ctcacgaggg tcttggattg agagagctga cacaatcatc atcacttcgt
     2401 gcaacgaacc gtttttcgcc gcctcgatca ccattctgcc taagcgagga tcgactggca
     2461 gttgggcgag ttggcgaccg attggggtta atctaaaccc atccccccgt ttacgggggg
     2521 aggttgggag ggggggagtg ccatcattag aacggatttc ccccctccct agccctcccc
     2581 cgcaagcggt ggaggggatt ttttcatttg gaataatcgc ctgtaattct tccaacaacc
     2641 gaatcccgtc ctgaatttgt cggctgtcag gggcatcaac aaacgggaaa ttggcaatat
     2701 cactcaatcc aagcgaagtc atttgtaaaa taaccgctgc aaggttagtt cgtagaattt
     2761 caggatcggt aaattgtgga cggctgttga aatcctcttc tgaatagagg cggatacaga
     2821 tcccttccga ggttcgccca caacgccctt tccgctggtt ggcggaggct tgcgaaatcg
     2881 gctcaatcgg caagcgttgc actttggtgc ggtagctata acgagaaata cgagccgtgc
     2941 cggtgtcgat cacatattta atattcggaa tagtgaggga ggtttctgcc acgttggtcg
     3001 ccagaataat tcggtttagc ccaccgcttg gctggaaaat ccgttgttgt tcggcagccg
     3061 ataagcgggc gtaaaggggt aaaatttcgc tatgtcgcag ttcctgcttt tgcaaggctt
     3121 cggcagtgtc acgaatttcc cgttcaccgt tcataaaaat cagaatatcg ccacgccctt
     3181 cggcttgcag ttcgtccacc gcatttagaa taccttgcag ttgatcttga tcttccacta
     3241 tcgggcgata acgcacttcc accgggaatg tccgaccaga gacttcaata atcggggcgt
     3301 tgttgaaatg tttggaaaaa cgttctacat caatagttgc cgaggtgatg atcactttca
     3361 gatcggggcg tttgtgcaga atttgtttca gataacccaa aataaaatcg ttgttcagcg
     3421 aacgctcgtg agcttcgtca ataatcagcg tatcgtactg atttaaatag ctgtcgtgct
     3481 ggatttccgc cagcagaata ccgtccgtca ttagcttgat taagctgttt tcactgactt
     3541 gatcgttaaa gcggactttg taaccgaccg tctcgcccag ttccgatttc agctcttcgg
     3601 caatgcgagt cgccaccgaa cgtgcggcaa ttcggcgggg ctgggtatgc ccaatcaagc
     3661 ccttcacccc acgcccgagt tctaagcaca ttttcggtaa ctgggtggtt ttgcccgaac
     3721 cggtttcccc tgcgatcacc accacctgat tttcgctaat cagttttaaa atttcctcac
     3781 ggcgagcgga aacaggcaga tcagggtagt caattttcag gttttgggcg tgctgagttc
     3841 gtgcgaaaaa acgtgcctga caagcggtga gatcttgcga aatttctgcc aaaactgacc
     3901 gcttgtgatc gatgttttta atattttccg ccccttttaa gcgaccgagc aggcggcgga
     3961 aatcggcggt ggtgatattt tccagcttca caagcggtgg tttcttatga tttttttgca
     4021 aaggtgtttc catttaattt ctcttttgca ttaagacctt gtgatttatt gtagggtggg
     4081 cgtaagccca cgtaacagaa tagggatttt atgcgtggac ttacgtccac cctacattag
     4141 gttttaccaa caatcagtga caaaatccct gccgcaatca acggcccgac cggtacgcct
     4201 tttaagaacg ccacgccgat caccgtgccg attaaaagcc cagttaccaa caccggctgg
     4261 ctgcccatta aattcacgcc cctgccacct agccacgcta ccagcacgcc agcaattatt
     4321 gagagtgcca ttttccagtt caatagttgg gcaagttcgg gaagcgtaat tcgccctgaa
     4381 accaaggggc taagtacgcc gatggttaaa atgataatcc cgatttttaa gccgtattga
     4441 tcgacaaagg ggataaattt agaaagtaag gtttgttgca taatcagcaa tacggcggct
     4501 gaaatagtaa ccgcactgtt ttggctgatc agccctaaaa taatcagcac cacaagtaga
     4561 agtgccactg cgttaaattg taaagtcata ggattaaaac gtaaaaaccc ttacttcata
     4621 gaagtaaggg agtggttaat taaacgttga gctagttaat aaatttcatt attgccatta
     4681 aaaagccaag aattgtgata tttaaggtaa ttgctttaaa tatcgtggta ttgatttcat
     4741 tttttagctt ttcaagttca agttgcaaat cagcttttgt tgctagtggg ctttgacttt
     4801 gctctaaggc ttcatcgaga gcattagcaa atgcctcagc catttcaggt gtttgattgg
     4861 cttcttgcaa gcgttttaca aaacgtaatt tgtcaaatcg taggctcata gctgcatcca
     4921 tttcaattat cctcttgtgg ttaagttata aactaattta gcataatcac aagcaggaat
     4981 tttaggagat tttgtgatct tcgtcgaaaa tcgacaattt agtttgccac catcaccatt
     5041 gccgggcgaa tcactcgtcc gtgaagcgtg tagccttttt gtagcactgt gctgacatgg
     5101 ttggcttcaa ttccttcggc aggttgcatt gaaatagctt cgtgcaattc agggttaaat
     5161 gcctcgccta cttcgccaat tgccaccaca ccaaatttag ccagtgtatt gatgaattct
     5221 ttatgagtca tttcaacgcc gtcaactaac gcttgagtgg tttcatcggt cacggcttta
     5281 tcaagggcat ctaatccacg ttcaaggtta tcaaccactg ttaacaattc tttggagaat
     5341 ttttctaaag cgaatttgtg agctttttca acatcttgct ctgcacggcg gcggatattt
     5401 tgaatttctg cttgagccct aagctgaata tcttgctcac gtttgtcggc ttctgcaata
     5461 taggcttcta actcggcaat tcgagcttga gcgagagcta acggttcttc ttgaggtgta
     5521 ttttgttctt ccgcttgagt ttcttcagtt acaacttcag gttgaatttc ttcttgtttt
     5581 acttcttctt gtaagtgttc attttgagtg gtcatttttt atcctttaaa agactaatct
     5641 ggaaatgtaa atcttcctta atatagcgat aaattaaggg atttcaagca tatctctttc
     5701 tttttgcgaa aataattcac tttttaatca gaaaaggctt gcaaaatacg ggggggggct
     5761 taaaatgccc tagtcttttc cgcccttagg caaggctata ttttttaatc acttttcttt
     5821 aacggagaaa acttaaatga aaaaattagc gggtttattt gcagcaggtt tagcaacagt
     5881 tgcattaaca gcgtgtaatg aagaaaaagc ggcagagcca aaagcaactg cggctgaagc
     5941 aaaacctcaa gcaacaaatc aaacagttca cctttatact tggactgaat atgtgcctga
     6001 aggtttgtta gatgaattta cgaaagaaac cggcattaaa gttgaagttt caagtcttga
     6061 atctaacgaa acaatgtacg ctaaattaaa attacaaggc aaagatggcg gttatgatgt
     6121 aattgctcca tcaaactact ttgtatctaa aatggcgaaa gaaggaatgt tagcggaatt
     6181 agatcacgca caacttccgg ttattaaaga attaaaccaa gattggttaa ataaacctta
     6241 cgatcaagga aacaaatatt cattacctca attattaggt gcgccgggta ttgcatttaa
     6301 tacggcagat tacaaaggtg aaacattcac ttcttggggt gatttgtgga aacctgaatt
     6361 tgcaggtaaa gtacaattat tagatgatgc tcgtgaagta ttcaacattg cgttattaaa
     6421 attaggtaaa aacccaaata caactaatcc ggatgaaatc aaagaagcct ttgaagagtt
     6481 gaaaaaatta cgtccaaatg tgctttcatt tacctctgat aacccggcaa actcatttat
     6541 tgctggtgag gtttctgtag gtcagttatg gaacggttct gtacgtattg cgaagaaaga
     6601 aaacgcaccg gttgatatgg tattcccgaa agaaggtcct gtgctttggg tcgatacttt
     6661 agccattccg gcaaatgcga aaaacaaaga aaatgcacat aaattaatca actacttatt
     6721 aagtgcaaaa gtagctgaaa aattaacgtt agcgattggt tacccaactt caaacgtaga
     6781 agcattaaaa gtattaccga aagaaattac tgaagataca gcaatttacc catctgcaga
     6841 ggttttacaa aaatctcaat ggcaagatga tgttggtgat gcaattgagc tttacgaaaa
     6901 atactaccaa gagttaaaag cagcaaaata atagctgatt aattctgaag cctctaggga
     6961 gcaatcttta gaggctttct cttttgagtt gcaaaaaatt tggcaaaaac gaccgctttc
     7021 taaggtacaa tcataaaaac atacttagat tttgagagat taaaccatga acttaagatg
     7081 gcaagaatat cctgaaaatg cccgcaaatt atttaaacta tccctaccca tttttatttc
     7141 tcagctttca gcttccggaa tggggctggc ggatattgta atggctggct tggtgagtga
     7201 tgatgatgtc tcggcgattg cggtaagtaa ttcgatttac tttccgctct ttctgtttgt
     7261 gtcagggttg ctgaatgcaa ttaccccaac cgtttcttac ttaaacggct cgagcaatcg
     7321 ccatttaatt gctaaccaag taaggcaggg ctattggatt gtgtttgcaa tgtctattcc
     7381 attaatggca attttcctaa acagccatct gattttagac ttaatgcaaa caccggctga
     7441 gttttcaatc aaatcgcaac aatatttagc agtaatggca gttgggctta ttcctgcttt
     7501 gctggcggtg agtttacgtt gtatgaacga tggattatct aaccctaaac ctgcgatgcg
     7561 gattaccttt ttcggtttat tgctgaacat tccgctaaac tatattttca ttttcggcaa
     7621 atttggcttg cctgaaatgg gagcggtagg ctgtggtgta gcaaccgcaa ttgtgaactt
     7681 gattatgttt tttatgttgt tgcattattg ctacaccaat tctgcccaaa aagatattcg
     7741 tttgtttgag aaatggattg atgctccaag cggtcaaatt ttgctaaaac tttgcaaatt
     7801 agggctgccg attgcctttg cgatttttac cgaagtgatg ctgttttcga cctcatcgct
     7861 gtttctgtca ccacttggtt cacaggtggt ggctagccac caagctgcat tgcaaaccag
     7921 ctcgctattt tttatgattc cgatgtcgtt tggcattgca accaccatta tgatcggaca
     7981 aacacttggg cagaaaaatg tggagggggc gaaaatctta agttaccacg cactgattac
     8041 cgcaaccgct ttagctatta ttgccgccat cattattatt ttgttgaaaa attttatccc
     8101 ttttgcgttt accaacaacc cgatttcgat tgcgattacc gctcatttat tgatttttgc
     8161 ggcggtttat caaataccgg atgccattca agcggtagca aacggcattc tgcgtggtta
     8221 taaacatacc caaccgattt tatacgttac tctgttttgt tattgggggg tgagtatgcc
     8281 gctgggctac attttggcaa gaaccgattg gattgtacaa ccaatggctg ctcaaggttt
     8341 ttggttagtc ttttgtatcg gcttaagttt ggctggtggc ttgttattat ggcaaatgcg
     8401 aaaaatacaa gcggtgccaa aagcacaatt aattgcaaaa ttagaacgga ttaaataaca
     8461 gattctctgc tatggaacat agcagtgctt tttactttta gaagataact ttgctgatga
     8521 aaaacctaaa tcctatccaa ctacctttga ataaatccgt tttaatcgaa gcgtcggcag
     8581 gtacagggaa aacctttacc attgccaacc tgtatttgcg tttattgctc ggcattggtt
     8641 gcaacccgtt aacggtggaa caaattttgg tggtgacatt taccaaagca gcaaccgaag
     8701 agttgaaaaa ccgtatccgt aaaaatattc agcaatgtgc cgacttttta aaagatcaag
     8761 ctgatggttt ggaagtggaa agtacaaaat cctatcgaaa caaccttgat tttttggcac
     8821 agatttatcc gctcattccg aatattcacg aagcattgtt acgtttaagt attgccgaac
     8881 gggaaattga taccgccagc gtgtttacca ttcacggctt ttgtcaaaaa atgctggttc
     8941 agtttgcgtt tgagtcgggc gtgcggtttg acttggattt acagccaaac caaagtgatc
     9001 tacttaaaaa attaagtgaa gaagtctggc gagagcaatt ctatccgcaa gatttagcca
     9061 tcacttatgc ggttgccgaa caacttggta cgccggaata tgccctcaat gcggtaaggc
     9121 gttatttatc tactgaatta cctgaaccaa atgcctcttt aaatcaagat attgccgagc
     9181 atttggctca atatcagcag tttattaatg aggtgaaatc ctattggaaa acgcattcag
     9241 atgaactggt tgaaactatc cgccacgatt taaagcataa aattacgctg cacggaggtt
     9301 ggtatcgagc gaccactttt gaagccggtg tggaagtgat gaaccagtgg gctaattcgc
     9361 aacataacca tttcccggca gggctttatt tattcactac agagaaaatt gcggctggca
     9421 cgaaaaagag ctgtgagccg atagattccc ctcatcttgc aaaaaatcag gcttttttga
     9481 ccgcttacaa ggaacgtttt gaaaacagct tgtcagcaat gttgcagtat caatttttaa
     9541 ttgctctcaa agcaaaattg gcagagtaca aacaatcgca cccacaacgc agttttgatg
     9601 atttactcac aatgctaaac caagcgttga aaaatctaca cacgggcgaa gccttgtctc
     9661 agaaaattcg ggcgatgtat ccttttgcga tgatcgatga gttccaagat accgatttaa
     9721 cccaatatga aattttcagc caaattttta tggcagagaa tgcagagaac ggctttatta
     9781 tgattggcga cccgaaacag tcgatctata aattccgtgg ggcggatatt ttcacgtatt
     9841 tgcgagcagc aaagcaggct gatgaacaat tcacattggg caaaaactgg cgttcgttgc
     9901 ctgaagtggt gcaagcggtc aataatttgt tttcttttgc aaatgataac tctccgtttc
     9961 tttatgagga tattggcttt cacccagtgg aggctgatag ccaaaaaggt cagttagtta
    10021 gtgagcaagg gcattttgta ggctacatca cggaaaacag taaattagat gatttggctg
    10081 aaatgtgtgc ttatcaaatt catcaacagc ttgaggcgat ggctcaaggc aaattcggag
    10141 ttgaaaaaaa cggcgaattt caatcctttg ctgcaaaaga tattgcgatt ttagtccgaa
    10201 aaggttcaca agctaagcga atcaaaaagg cgttggcaaa acgtaatatc aaatccgtct
    10261 atttagctga atcagcttct gtatttggtt cggaaattgc aacagaattg tgttggttat
    10321 tgcaagccgc actgaatccg tatcattaca aggcattatt atcggcgtta ggctcaacct
    10381 tgtggggctt aacggcggca gagatccacc gctataagga aaatgagcaa ctttgggatc
    10441 tgaaagtcaa tcagtttgtg gaatacagca acatttggaa agagcagggc gttctgccga
    10501 tgttgcataa aatctacttg gagcagggca ttatcgaacg tttgaaaggt ttggaaaatg
    10561 cggatcgctt gattacggat ttattgcatt taaccgaaat tttgcaggaa acctcgcaag
    10621 cgttagagtc tgaagctgaa ctgttgcggt ggtatgaaaa gcaagttaat tcgcccgatt
    10681 ttaatgacga gcaacaactt cggttggaaa gtgaagagga gctaatcaaa attgtgacga
    10741 ttcacggctc gaaaggcttg caatatccaa ttgtttggtt accttttgtt tcacaaaaaa
    10801 gtgagggaga cacgcagaca gcattaagtt tatatcgcaa ttctcaaggt gcgctggatt
    10861 ggtgtttagg ctcagaagag gcaagttttt cgctacagta tgaggcttat gcggaagatt
    10921 tacgtttgct gtatgtggca ttaacccgcg cggaatctca gcttaactta gtgttgccga
    10981 aagagatttc cggctggtct ccactccatt acttgttagc agacggggaa atacctacag
    11041 gaaaagtgcc tgtttcagat tattttagcc aaaaacggat tgctgctaat ttggttgaag
    11101 tagccgggga attacctttc tcactgtggg aaggagaaca atcttcagac gattaccaag
    11161 caaaaacctt tagtggcaat atccgtgtgg ttggtcaaat taccagcttt acttcacttt
    11221 atgcccaaca cgagcgtttt agtgaaggga aatcagaaag ggtaatggat ttagcccaag
    11281 atcgagatgt acatatagaa accattgagg caggttattc aagtgaaact gccgctttct
    11341 cgccattcca attcccacac ggtattaaag tgggcacggt gttacatcac ttttttgagc
    11401 attgccaatt taacgaacaa attgatagag aggctgttgc taaagtatgc gagcaattag
    11461 ggctaagcga ggaatggatt gaaccaacag cattatggtt tgagcgtatt ctaactaccc
    11521 ctttggcaga ggcaaatttc tgcttaaaag cgattgatga aaccaaacga ttaaacgagt
    11581 ggcaattcta tttacgctta aaaaatgata aagccttaca tcaattaaat gcgttattaa
    11641 agcaacatag ccctttagca aaaactttac ctgaattgca attaccgcaa ttagaaggtt
    11701 ttgtgcgagg ctttgtagat tgtatcgtgc aagtggagga gaaattctac ttaattgatt
    11761 acaaatcgaa tttcttaggc tatctgccac aaaattatgc gaaagaacac attcaacggg
    11821 aaatgggcag acaacgctat gatttgcaat atctgcttta cactctcgcc ttacaccgtt
    11881 atctaactgc ccgtttaggt gaaaaatacg attatgaacg agacttcggc ggagtggctt
    11941 acttgttctt aagggcaatg aacggcgaaa atgataacgg cgtttatttt gacaagccga
    12001 ctaagcagtt gattgaagaa atggatcggt tgtttgggta aatatgtgta ggggcgaaaa
    12061 tttttcgccc ctttatgggt aattaactcg cttgctcata ccaaatctta ttgatataaa
    12121 gcaacacttt accaagcgga gtttctaccc taatttcatc atcaacctgt ttgccgatta
    12181 aggctcgggc gacaggggag tcaatcgaaa tccagttttt tgctgggtcg aactcatcgc
    12241 aaccaacaat acggtactgt tttgcctcgc cctcttcatt ttctaattcc acccaagcgc
    12301 cgaaaaagac tttgccttct tgtttagggt gataatcaac aatttgtagc acctctagcc
    12361 gcttagataa aaaacgcaca cggcggtcga tttctctcaa acggcgtttg ccgtaaatat
    12421 attcggcatt ttcgcttcga tcacccaatg cggcagcatc agacaccgct tgagtcactt
    12481 tcgggcgttc ttcccgccat agaaatttta attcctgatc taaggcttgc cagcctgaac
    12541 gggtaatgta atttgattta gccatagtta tttaattgtt tatggagcaa atagtgattt
    12601 gcccctacga agtttctatt ttgaaagcaa taaattcgct actgttctta ttcccagtcc
    12661 ggttgctcct gccgcccata aatcgctcgc cgttttgcgg aatgtggcag aggcgtcaat
    12721 atgtagccag ttttgctggt agttttctac aaagtaagat aaaaatgcgg tggcggtact
    12781 tgccccagcc cccaccggca cagagccgat attggcgatg tctgcaaagg aagaagcaat
    12841 ttgcccacgg tgaaattctt caaacggtaa acgccagaac ggttcatttt ccgttttggc
    12901 agacttaaat agtgcttcgg ttaggttgtc gtccattgaa agcaccgagt ggtagtcatt
    12961 gccgactgcg acttttgctg ctcctgtgag ggttgcagca tcaataataa attgtggatt
    13021 ttgcttgcac gcatcaatta aaccatcggc aagcactaaa cgcccttcag catcggtgtt
    13081 taaaacttca acagttacgc cattgcggta agtgataata tcgcctaatt tgaacgcatt
    13141 gtggctgacc atattttccg cacagcagag gtagagtttc actcgtttat tcaagcctcg
    13201 agcaatggca aaagcaagtg agccggttaa aagtgccgct ccgcccatat cggttcgcat
    13261 tgtgctcata ctatcgcttg gttttagact ataaccgccg gtgtcaaagg taatgccttt
    13321 gccgaccaag caagccagta cctgtgcgtt ttcatcgttg gttgggttga agtctaatac
    13381 taagaatgca ggcggattgg cagagccttt tccaactgtc caaatgccgt gatagccttg
    13441 ttcttgtaat gcttcgccac gaatgatctg gtaagaaacc gtattcggtt ttgccatttt
    13501 ttggataaac tcgaccgctt gttcggctaa tttttcaggg gttaatttat ctgatggctt
    13561 attaattaat tcacgcacga aagtggcaca ttttacacgg ttatcaaatt cggcttgctg
    13621 ttcgtctaag tctgggtatt gtaccgagcc ttgatttttt accgaatgaa atccttgatg
    13681 aaacgcccag caggattcta aatcccaact atcgccggtc agctccacgg ttgaaatacc
    13741 ttgattttta attttgcggg cggctttttg aatagtggct aaatcgttgt tcggattatg
    13801 tacggtaaac cccgcatcat ttgcggaaag aatagcattt ttaccccaaa tttcaggggc
    13861 gttttgagta gaaagtgaaa ttttcattgc tgtcattatc ggtttcctta gaaaatggat
    13921 tttgctttat cctaacaaac ttttgcaaag ttttatacaa aaatgaccgc ttgcagagca
    13981 tagccttgac aagcggtcat tgtttcgtgt ttttacaacc tgattaagga cggattattt
    14041 gccgtcccaa gctttaattg cattgtggcg gattttaaca cggctttcag cattgccttt
    14101 gtagtaatct ttatctaaac cgccttccca gtcgccatag ttcgggttcg gtaacataat
    14161 gaatttttta ccgaattgtt ggctgttttc ggctacgaat gcacggcgtt ccgcgtttga
    14221 tttacggtaa gtcgcatcgc cgaaatcgtt taagttgtcg ccaatataga gcacgatttc
    14281 atagccttgt ttttcgattt cttcaaagcg aggggttttg ttcgatttgt cttttttcaa
    14341 caagagtgtt tgttcgctca caccactaaa gcctaatttg ttcatatcat caatagtacc
    14401 ggctttttca ctgctgtctt tacggtttga aacatagaac atagttcctt tgtggctatt
    14461 aacgtagttg ttaaattcca ctgcaccggc aatcgctccg gtttggcggg catttaccca
    14521 acgagtccag ctttcaccat caaacggctt gtggttttta acctgccagc cggcataaag
    14581 gctgttgtcc accatagttt catctaaatc tactaccacg gcttttttct tgcctttttt
    14641 cactttggct tggtcgaatg ccactttcgc tgcattgaat gcttgatggg caagagcttg
    14701 gtattcgccg gattgttgca tccagtttat ccctaatgta gcttgctctt gcaacaccat
    14761 ttgatgatgc tcatcgctgt ttttatgcgt gtgcgaacaa gcagttaata caaataaagc
    14821 tgtgaggctc atagcagaaa gtttgatgtt tttcatacgt atatccttag ttttattgtt
    14881 gataaacaga gcgattatac ctaaaaagag ttgcttatgg ctaatagttt attgctccta
    14941 ttcactttta aagcaaacta aggtagccta gtaacctttt tataacaatt acacagaggc
    15001 tatatgcaaa cattattaaa atttacacaa tttatcagca aaaccttcgc catttgggtg
    15061 gtgttttttg cttttatcgc agcacaattt ccggaaacct ttaagcaatt cctgccttgg
    15121 attccttatc tgttagggat tgtgatgttg ggaatggggt taaccttaac tttcaaagat
    15181 tttgccgagg taacgaaaaa tccgaaatca gtgattatcg gcgtgatcgc tcaattcgtg
    15241 gtatgccgag tgtagcttat ggattggcaa aagcatttaa cttacccgca gatttagcca
    15301 ttggtgtgat tttagttggt tgttgcccgg gcggtacttc ttcaaacgtg atgacctatt
    15361 tggctcgtgg caacacagcg ttatcggtag cttgtaccac tatttcaact ttacttgccc
    15421 cagtgctcac gccggcaatt ttctatttat ttgccagcca atggttagaa attaatgcaa
    15481 cggcaatgtt tatttcggtg ttgcaaatgg tgctattgcc aatttttgtg ggcgtggtga
    15541 ttcgtactat tttcaaacaa aaagtagagc aatttagcca aacaatgcca ttaatttcag
    15601 tgattgcaat cgtattaatt gtcacggcgg tggtgtcagt tagccgagat cgtattattg
    15661 aatccggttt attaattttt gcggtagtag cattacacaa cggtttaggt tatttaatcg
    15721 gttatttggc gagccgttta ttaaaattac cgattgcaga cagcaaagcg gtggcgattg
    15781 aagtggggat gcaaaactca ggattggggg cagcacttgc cgcattacac tttaaagcca
    15841 accctatcat tgcggtgcca agtgcggtat ttagcttctg gcacaatatt tcaggcccga
    15901 ttttggcgat gattttcgct aaaatgaaaa acgataacga aaaataaaat tcgttttaat
    15961 acagacgttt taatgtttta ataaagaata gacaaacggt taaatttttc taattttttg
    16021 caaaatgttc agaaaattta accgcttgtt gtttagaatt tagaacattc ttggtccacg
    16081 tgcagtgctg attttttcat catccgctct ttgggtaaca atcacttgat caccctcatt
    16141 taagcctgct aatacttcgg tattttgcgt atccgaaatg ccaatttgga tttttctttc
    16201 aaccgactgc tcattttcca gcaccaacac ataattctct ttaccacgtt ttttgattac
    16261 gctggtgggt acaactaaaa cctgttcccg tttatcaata gtaatttggg cttgagtagt
    16321 cattccgatg cgtaaactgt tatcggcatt atcaacggaa acattagcgt agtaataaac
    16381 cgcttcggta ttacccgatt gttcggtgta gttattatcg gtcagcgtgg tgagtgccgg
    16441 atcaactgaa tcaatggtag cactatattc tcgatttggc tcagcaaggg tggaaaactt
    16501 aactttttgc ccgactttaa cctgtgcaat atcgccctcc gaaatttcaa gtttaatcag
    16561 catcttagat aaatctgcta cctgtacaat agttggcgaa gtttgattgg aattgaccgt
    16621 ttgcccgaca gaaaccggta cagacacaat cacgccgtcc attggcgaga taatttgcgt
    16681 ataagttaag ttggttttag catcgtttac cgaaatttca gccgatttta attgtgcttt
    16741 aatgttatcc accgtggctt tggctgaagc taaggtattt ttagccgttt caagatcgtt
    16801 taaagaggca ctatttgccc gatagagttt atttaagcgg ttgtagttcg attgagctac
    16861 ttcaagtgca acctgtgcag aggtgagctg tgctttataa gaagaaagcc gggcttgagc
    16921 ggtgcttaag gtgttgctct ggttgctgga atcaatatct gcaattaaat ctccttgctt
    16981 caccgattgc ccgagctcga catagagttt ttggattttg cccgaaactt gagcccccac
    17041 ttcggtgcgt ttattcgctc gaaccgaacc ggttgccagc acatttttgt taatttctgc
    17101 ccgtgtgaca gcttgggtaa tgtaatggat ttgcggttta ttggtttgtt gctgataata
    17161 aaaatagccg cctattccga gagcggcaat tagcacaaga gtggctaatg ttttacgttt
    17221 catactgttc cttgatgata tacaagcggt caaatttaga caaaaatttg caaaaaagtt
    17281 gctctcaact aaacaatgcc aaatgtggct cgaagatagc ttgccaccgc ttcatctttg
    17341 ttaaagccaa tcacttcatt attcggtaat acggctttta aacgtggatc ggcattaccc
    17401 attaaacaac ctttgccggc taaacttagc atttccacat cattcatacc gtcaccaaaa
    17461 gcgatacaat cggctaaggt gtagccccgt aatttgacca attcggcgag agcattggct
    17521 ttacacactc cttttgccat cacttcaagg caaagtaggg cagagtaggt catttgcaca
    17581 cggtcgccat aggtatcccg aatataatgt tcaatcggta ctaaagcttc agcagttttg
    17641 ccgataaaaa acactttctc ggtatttcga ccgtgatgat gagcaaaatt tactacttga
    17701 tagctaaatc ctgaatcttg atggaatttt tttaactctg caatctcttt attgataaac
    17761 cattcatcgc cttgatagct gtttaagcac acattttggt cgtcaaattc aatgttcatt
    17821 aattcaaagg caagatcttc cggtaaataa tggttgagaa tttttttacc ctctaaattg
    17881 ttggctcttg ccccgtttga agtgactaac attgcatttt taacgtttac tttcccaata
    17941 atctgagaca catctggatg attgcgaccg gtggcgaaaa aaatatcaac accttttttt
    18001 gatagttgtt ctaatgtatc aatagtgaaa tcaccaattt tatgattggc atttaatagt
    18061 gtgccatcaa gatcggaaat aattgcacga aatgg
//
LOCUS       DANLZC010000040        17608 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.040, whole genome shotgun sequence.
ACCESSION   DANLZC010000040 DANLZC010000000
VERSION     DANLZC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17608
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.040"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            76..1209
                     /gene="carA"
                     /locus_tag="PXE44_001039"
     CDS             76..1209
                     /gene="carA"
                     /locus_tag="PXE44_001039"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL5588561.1"
                     /translation="MFKPAILVLTDGSVFSGKSIGADGHTIGEVVFNTAMTGYQEILT
                     DPSYTKQIVTLTYPHIGNTGINLEDKESPNVCASGLIIRDLPLLHSNFRASSSLSDYL
                     IQQNIVAIADIDTRRLTRILREKGAQAGCILVGDATEENIANALAFANSFGSMAGKDL
                     AQSVTCNELYKWTDGEWQLGKGYVPQQQTEFHVVAYDYGVKHNILRMLAERGCRLTVV
                     PAKTSAEAVLALNPDGIFLSNGPGDPEPCDYAITAIRKLLETQKPIFGICLGHQLLGL
                     AAGGKTKKMVFGHHGANHPVQDLDSQKVLITSQNHGFEVDESSLPSNVRVTHRSLFDG
                     TVQGIELTDRPAFSFQGHPEASPGPNDVAYLFDRFVSEMKKVK"
     gene            1312..4518
                     /gene="carB"
                     /locus_tag="PXE44_001040"
     CDS             1312..4518
                     /gene="carB"
                     /locus_tag="PXE44_001040"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL5588562.1"
                     /translation="MPKRTDINTILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     VLVNSNPATIMTDPNMADVTYIEPIEWKTVEKIIEKERPDAILPTMGGQTALNCALDL
                     SKNGVLKKYNVELIGATEDAIDKAEDRGRFKEAMTKIGLNTPKSFVCHSFEEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNRDEFIAICERGFEASPTHELLIEQSVLGWKEYEM
                     EVVRDRADNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAINPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLNE
                     LRNDITGGLIPASFEPTLDYVVTKIPRFAFEKFANADDRLTTQMKSVGEVMAMGRTFQ
                     ESLQKALRGLETGICGFNLLSEEPEKIRRELANPGPNRILYVADAFGAGFSLEEVHHY
                     SKIDPWFLVQIQDLVLEELALEKKQFSDLDANEIRRLKRKGFSDKRIAQLTKVSEKMV
                     RDYRHTYAILPVYKRVDTCAAEFASDTAYLYSTYEEECEAKPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHASLALREAGYETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RVENPWGVIVHYGGQTPLKLANALSDAGVNIIGTSADSIDAAEDRERFQQILNTLGLK
                     QPANRTARNALEAVALANEVGYPLVVRPSYVLGGRAMQIVYNDEELNRYMREAVSVSN
                     DSPILLDHFLNNAIEVDVDCICDGENVLIGGIMQHVEQAGIHSGDSACSLPAYSLSGH
                     VLDEIRKQTNAMAFALGVKGLMNVQFAVQNEVVYVLEVNPRASRTVPFVSKATGRPLA
                     KIAARVMAGETLADLNALEEIIPSKFFVKEAVFPFIKFPGVDTILGPEMRSTGEVMGI
                     GNTFAEAFFKAQLGAGERIPKVGKVFMSVIEEHKPALIYIAKRFQEQGYGLCATFGTA
                     NFLRQHGIAVQTVNKVREGRPHIVDAIKNGEIALVINTVSGEPEVIADSHSIRRTALQ
                     QRVPIYTTLAGADAISYGVKHINEFDVYSVQELEQKAN"
     gene            complement(4593..5384)
                     /locus_tag="PXE44_001041"
                     /pseudo
     CDS             complement(4593..5384)
                     /locus_tag="PXE44_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            5587..6585
                     /gene="yiaK"
                     /locus_tag="PXE44_001042"
     CDS             5587..6585
                     /gene="yiaK"
                     /locus_tag="PXE44_001042"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDL5588563.1"
                     /translation="MRVSYEELKAQFKRVLLARNVREEIAEECATVFADTTQAGAYSH
                     GVNRFPRFIQQLENGDIVPEAVPTKVLSLGAIEQWDAHQAIGNLTAKKMMDRAMELAS
                     ENGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLI
                     IAVPTNPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNLRL
                     MPMGFWKGSGLSIVLDMIATLLSNGESTAAVTEDKDDEYCVSQVYIAIEVDRLIDGKT
                     KDEKLNRIMEYVKTAERADPDVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQAL"
     gene            6710..7075
                     /locus_tag="PXE44_001043"
     CDS             6710..7075
                     /locus_tag="PXE44_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL5588564.1"
                     /translation="MFFGHISQVKANRYPPAIQIALNYLKNTDFDAMEAGVYELKGRQ
                     IYVQVLDLNTKSKHEFQPEVHRNYLDVQYLHRGKEIMAAAVDTGTNPIAMEYNPERDI
                     QYYQSVANENEFRCVEGNF"
     gene            7139..7618
                     /locus_tag="PXE44_001044"
     CDS             7139..7618
                     /locus_tag="PXE44_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL5588565.1"
                     /translation="MKALAQLVGKLLEILVVVILSAMSILVFVNVVLRYGFNSSISVT
                     EEVSRYMFVWLAFLGAVLAFSENQHVKVTMLTDKLSPTARNILSVVTDLAMLFCCYLI
                     VDGSWIQFNLNLNNFAPISGLPQGITFLASTVAGVLIGLLIIARIVATVGVIAKGEK"
     gene            7619..8896
                     /locus_tag="PXE44_001045"
     CDS             7619..8896
                     /locus_tag="PXE44_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDL5588566.1"
                     /translation="MTVVIFLSVLLGSILLGIPVAFALLVCGVALMLHLDLFDAQILA
                     QQIVSGADSFSLMAIPFFILAGEIMNEGGLSKRIIDLPMKLVGHKRGGLGFVAILAAM
                     IMASLSGSAVADTAAVVAMLLPMMKTTGYPIDRSAGLIGTAGIIAPIIPPSIPFIVFG
                     VASGVSITKLFLAGIFPGIMMGICLAALWWWQAKRLDLMTFSKATKEELCISFKNSIW
                     ALLLPVIIIGGFRSGMFTPTEAGAVATFYALIVSLFVYREMKFKDLYKVVLAAAKTTA
                     VVMFLVAAANVTGWLITVAELPMMLTELLEPLIESPTTLLLVIMLAVFIIGMVMDLTP
                     TVLILTPVLMPLVEEAGIDPVYFGVLFILNTSIGLITPPVGNVLNVITGVSKLPFDQA
                     AKGVMPYLVMMICLLLLFVFFPSLVLVPLSWMQ"
     gene            8999..9985
                     /locus_tag="PXE44_001046"
     CDS             8999..9985
                     /locus_tag="PXE44_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5588567.1"
                     /translation="MKLFNLKTLFALVAGVAVMSGSAMAETSLRFGYEAPRSDTQHIA
                     AKKFDELLAAKTKGEIKLKLFPDSTLGNAQTMISGVRGGTIDIEMSGSPNFTGLEPKL
                     NVIDIPFIFKDREHVYKVLDGEVGQGLLKDLEAQGLKGLAFWDVGFRAFSNSKHPVTK
                     PEDIKGLKVRTNQNPMYIQAFTLLGGNPVPMPLSELYTALETRAVDAQEHPIGIFWSA
                     KLFEVQKHLSLTNHGYTPLIVVMNKAKFDGLAPELQTALVEAAKEAGQFQRDLNVKNE
                     KEIIEKLRKQGIEVIEQVDNAPFKAAIEAEVRKAFVEKHGDELLKKVDALAQ"
     gene            10059..11042
                     /locus_tag="PXE44_001047"
     CDS             10059..11042
                     /locus_tag="PXE44_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5588568.1"
                     /translation="MKLFNLKTLAAFVAGFALFSTAQAETSFRFAYEAPRSDTQHIAA
                     KKFNDLLQEKSQKGLKLSLFPDSTLGNAQTAISGVRGGTIDIAMSSSSNFTGLEPKLN
                     VIDIPFIFKDRDHAYKALDGEIGQGLLTALEAHGLKGLAFWEVGFRAFSNSKHPITKP
                     EDIKGLKIRTNQNPMYIQAFSILGANPVPMPLSKLYTALETKAVDAQEHPIGIFWSAK
                     LYEVQKYFSFTNHGYTPLIVAINKAKFDALPAEQQTALVEAAKEAGKYQRQLNLENEQ
                     KIIDSLKKAGIEFVDNLDTTPFKDAVSAETRKAFIEKNGDGLVKAIDALAK"
     gene            11173..12657
                     /locus_tag="PXE44_001048"
     CDS             11173..12657
                     /locus_tag="PXE44_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5588569.1"
                     /translation="MQHIIKRFGSHTAVNDVSLDIHSGEIVALLGENGAGKSTLIKIL
                     AGIYDRDEGDILFHNQKIQSAHAIEKGNKKPIAFIHQDLGLIEWMTIAENMAFVMGFP
                     RKFGLIDWAKVREQSQKALDFVGIKLSADTRVFDLSRTEKALLAIARAIAVNAEILVL
                     DEPTASLPASDVEHLFTVLNRLRSQGVGMIYVTHRLDEVIAISDRILVMRDGFPVAEG
                     QTKLYDVKGLVKAIVGEESRGRQRVDLPTDTPEILSLQNVVVGDTGPVNFRLHKGEMI
                     ALAGLRGAGQEEIGRLLFGERTLDKGEIQLDKRSYSAKDPQDAIAKGVALVAGDRTRE
                     SVFMSMTTSENLFINPVLSGHSPLKRYSRREEWGESWYKFQLFDIRPKNLFIDASALS
                     GGNQQKIVLARWMHLNTPVLILEDPTAGVDVGARAEIYDLLNKALAEGVAIIVISTDF
                     EEIAHLCNRALVFNRGEIAGELLNEEVTFANLLALASDSQSKKQ"
     gene            12852..13898
                     /locus_tag="PXE44_001049"
     CDS             12852..13898
                     /locus_tag="PXE44_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5588570.1"
                     /translation="MAQTNIKSTALEQPVSIARDGFMAWFSQMSTRYGLLWLCVLLVI
                     IFSLTTESFASMLTLNAILESKSKIALLALAATTTMIVGKIDLNVGFGIVLWHILVIT
                     LQVQYGFSWYMAILIVLVIAALYGLLNGILVALADIDSFVATLGSGTVLYAVALWHSG
                     GRQIVGDLPDEFIALNSTEIFGIPISAFYVLAVAIVMWLVTEHTPTGRCMYAVGGNPT
                     AARLNGINIKKYTIVPFIVSSVITAFTGVLISAQQGVGQASVGMDYLLPALVGAFLGS
                     TTIRPGRINVWGTVVGIAILAIGISGIQQFGGAFWVEPLFNGATLLLSITIAGYAQRK
                     RLLNQKAVQKKSTN"
     gene            13911..15020
                     /locus_tag="PXE44_001050"
     CDS             13911..15020
                     /locus_tag="PXE44_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5588571.1"
                     /translation="MKKAFRTTLTAIATLTAIAFGNNAFANDDPFVQEAKQLVAAATA
                     KQEKWDGPTTGPQLQKEKTVIFIASDMKNGGVLGVIDGMKEAVGVTGWKLDVLDGAGT
                     VNNQLAALNQAIARKPDAIVIGGWNPNVAKIPLQKATKNGIKLAAWHATPEPGAIDKY
                     GIFYNVTSDSDEIARLSAMLAVAESNGKAKAIILTDSLYEIALRKANVMKEVIEKCKE
                     CKVLEFIDTPLADTSSRMPNLTFSLLQKYGDDLEYALAINDLYFDFMAPSLRTAGKDK
                     PFSISAGDGSVTAYQRIRNGDKQFATVPEPLNLHGWQLVDELNRAFANEKPSGYITPA
                     HLVTKENVAFDGGENNLYAPQNGYKGAYKAIWGVK"
     gene            15125..16015
                     /locus_tag="PXE44_001051"
     CDS             15125..16015
                     /locus_tag="PXE44_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDL5588572.1"
                     /translation="MSNKFIPKISNPRFNELVGNTFELEELFDKAIWAEGPAWSKPQQ
                     ALYFSDVKGCTMYRWTEQDGTQVFRQPSYFSNGNAFDANDNLITCEHGNRGISLTTPS
                     GEYRFLVGQLDGKRLNSPNDVVVKSDGIIWFTDPPYGILSDNEGKQSPSEIIGCYIYC
                     YDPKTDKLNIATFNTMRPNGLAFSSDEKQLFVADMSSVEFDEDGLHHLVVFDVDGNKL
                     KNRRDVAEISPGIPDGFRIKQNGVIFCSCEEGIIALLPDGTELGRFVLGKLTSNCSFG
                     DDEQTLFITCSNSVYRLTIK"
     gene            16087..17556
                     /locus_tag="PXE44_001052"
     CDS             16087..17556
                     /locus_tag="PXE44_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDL5588573.1"
                     /translation="MDNKMNYTLGIDCGGTFIKAALFDTQGNIQALHRENVSVISEQA
                     GYAERDMSQLWAICAEVVRSTIEKSGVNPADIKAVGISAQGKGAFLLDKQNRPLGRAI
                     LSSDQRSLEIVKQWQAEGIPQQLYPITRQTLWTGHPVSILKWVQQNEPERYAQIGAVL
                     MSHDYLRFCLTGELHCEETNISESNLYNMEKGEYDQQLADLLGMPDVLEKLPPVIEPN
                     KISGYVTENAAKLTGLAVGTPIVGGLFDVVSTARCANLDDETKLNAVLGTWSVVSGIT
                     DHIDASQSLPFVYGRYAEAGKFIVHEASPTSAGNLEWFVNQWKLDYVKINQAVAQLPP
                     AASSVLFVPFLYGSNAGLGMQAGFYGMQAHHTQAHLLQAIYEGVLFSLMSHLNRMFIR
                     FPKADVLRVTGGPVKSKVWMQMLADLTGMRLEIPQMEETGCLGAALMAMQGVGLDISQ
                     VQALNNEMQVFEPNPENYAAYQTKYQRYQKLTEALNTMI"
BASE COUNT         4928 a         3862 c         4144 g         4674 t
ORIGIN      
        1 aacgtttgct tgattaaact tagtcgccta cttctaaggc tacacagata aaatttgatg
       61 attaggagta aaacaatgtt caaaccagca attctggttc tcactgacgg ttctgtcttt
      121 tccggcaaat ccattggtgc tgacggtcac actatcggtg aagtggtatt taacactgcg
      181 atgaccggtt atcaagaaat ccttaccgat ccttcttata ctaaacaaat agtcaccctg
      241 acttaccccc acattggcaa taccggcatt aatttagaag ataaagaatc ccccaatgtt
      301 tgtgcctccg gcttaattat tcgtgatctt cctcttctcc atagcaactt ccgtgcgtcc
      361 tcctcacttt cagattatct cattcaacaa aacattgtcg ctattgccga tattgatacc
      421 cgccgtttaa cccgaatttt acgcgaaaaa ggggcacaag ccggttgtat tttagtaggc
      481 gatgccacag aagagaatat tgctaacgct ttggcatttg ccaacagttt cggttctatg
      541 gctgggaaag atttggctca aagtgtcacc tgcaatgaat tatataaatg gacagatggt
      601 gaatggcaat taggtaaagg ttatgtaccg caacaacaaa ccgagttcca cgtagttgcc
      661 tatgattacg gtgtaaaaca caacattttg cgaatgcttg ccgaacgagg ctgtcgctta
      721 accgttgtgc cggcaaaaac ctctgctgaa gctgtgcttg cgttaaatcc ggacggtatt
      781 ttcctttcta acggcccggg ggacccggag ccgtgcgatt atgccattac cgcaatccga
      841 aaattattgg aaacgcaaaa acccattttc ggcatttgct tagggcatca acttttaggt
      901 ttagcggcag gcggtaaaac caaaaaaatg gtgtttggtc atcacggagc taatcacccg
      961 gtacaagatt tagacagcca aaaagtgctc atcaccagcc aaaaccacgg ttttgaagta
     1021 gatgaatcca gcttaccatc taatgtgcga gtgactcacc gctcactgtt tgacggcacg
     1081 gtgcagggga ttgaactcac tgatagaccg gcgttttcgt tccaaggtca cccggaagcc
     1141 agcccggggc cgaatgatgt tgcctattta tttgaccgat ttgtcagtga gatgaaaaaa
     1201 gtgaagtaag gacgtaccta tgtgtgtcca tcaagcggtc gaaatttgca aaaaacttac
     1261 caaaaatgac cgcttgtagc ctgaaataaa cgagataaaa gagagcccaa aatgccaaaa
     1321 agaacagaca taaacactat cctaattatc ggtgccggtc cgattgtgat cggtcaagcg
     1381 tgtgaatttg actactccgg agcccaagcg tgcaaagcgt tgcgtgaaga agggtataaa
     1441 gtggtgttgg tgaactccaa ccctgcaacc attatgaccg acccgaatat ggcagatgtg
     1501 acttacattg agccgattga atggaaaacg gttgaaaaaa tcattgaaaa agaacgccct
     1561 gatgccattt taccgactat gggcgggcag acagcgttga actgtgcctt agatctttcg
     1621 aaaaacggtg tgctgaaaaa atataatgta gagctgatcg gagctaccga agacgcgatt
     1681 gataaagccg aagatcgtgg tcgttttaaa gaagcaatga ctaaaatcgg cttgaatacg
     1741 ccaaaatcat tcgtttgcca cagttttgaa gaggccttag cagcacaaga gcaggttggc
     1801 tttccaaccc tgattcgccc ctctttcacg atgggaggct ccggcggtgg tattgcttat
     1861 aatcgagatg aatttatcgc catctgcgaa cgtggttttg aagcctcgcc tactcacgaa
     1921 ttactgattg aacaatccgt actaggctgg aaagagtatg aaatggaagt tgtgcgtgac
     1981 cgtgccgata actgcattat cgtctgctcg attgaaaact tcgacccgat gggtgtacac
     2041 accggcgact ccattaccgt tgctccggca caaacgctaa ccgataaaga gtatcaaatt
     2101 atgcgtaacg cttcgctggc ggtattgcgt gagatcggcg tggacaccgg cggttcaaat
     2161 gtgcaatttg cgattaaccc tgcaaacggc gagatgatcg tcattgaaat gaaccctcgt
     2221 gtgagccgtt cttctgcact cgcatctaaa gccaccggct tcccgattgc caaagttgcg
     2281 gcgaaattgg ctgtaggcta cacactcaac gaattgcgta acgacatcac cggagggctg
     2341 attccggctt cattcgagcc gactttagat tatgttgtga ccaaaattcc acgcttcgcc
     2401 tttgaaaaat ttgccaatgc agacgaccga ttaaccactc aaatgaaatc ggtaggcgaa
     2461 gtaatggcaa tgggaagaac cttccaagag agtttacaaa aagccttgcg aggcttggaa
     2521 accggcattt gcggctttaa cttgctttcc gaagagcctg aaaaaatccg ccgtgaactt
     2581 gccaaccccg gaccaaaccg tattttgtat gttgccgatg cctttggagc aggcttttca
     2641 ttggaagaag ttcaccacta ttctaaaatc gacccgtggt tcttggtgca aattcaagac
     2701 ttagtacttg aagaattagc gttagagaaa aaacagttta gcgatctaga tgctaacgaa
     2761 atccgccgct taaaacgcaa aggcttctca gacaaacgta tcgcacagct taccaaagtc
     2821 agcgaaaaaa tggtgcggga ttaccgccat acttacgcta ttctaccggt gtataaacgg
     2881 gtagatacct gtgcagccga gtttgcctca gatacggctt acctctattc tacttacgag
     2941 gaagagtgtg aagccaaacc gtctgatcgc aaaaaagtga tgattttagg cggcggacca
     3001 aaccgtatcg ggcaggggat tgaatttgac tactgctgcg ttcacgcctc gcttgctctg
     3061 cgtgaggcag gttatgaaac cattatggtg aactgcaacc ctgaaaccgt ttccaccgat
     3121 ttcgacacct ccgaccgcct ctatttcgag ccgctaacat tagaagatgt actggaaatt
     3181 gtgcgtgtag aaaacccgtg gggcgtgatt gtgcattacg gcggtcaaac accactgaaa
     3241 ttagcgaatg ccttatctga tgccggtgta aacattatcg ggacttccgc cgacagcatt
     3301 gatgccgccg aagaccgtga acgtttccaa caaattctaa acactctcgg tttaaaacag
     3361 ccggcaaatc gcaccgcccg taatgcgttg gaagcggtgg cattggcaaa tgaagtaggc
     3421 tatccgctgg tggttcgccc atcttatgtg ctaggtggtc gggcaatgca aattgtgtat
     3481 aacgatgaag agctaaaccg ctatatgcgt gaagcggtat cggtttccaa cgacagcccg
     3541 attttactcg accacttcct caataatgcg attgaagttg atgtggattg catttgtgac
     3601 ggcgaaaatg tactcattgg cggcattatg caacacgttg agcaagccgg tattcacagt
     3661 ggcgatagtg cctgctcact acctgcctat tcgttaagcg gtcatgtttt ggacgaaatt
     3721 cgcaaacaaa ccaacgcaat ggcgtttgct ctcggtgtga aaggcttgat gaacgtacaa
     3781 tttgcagtac aaaatgaggt cgtttatgtg ttggaagtta accctcgagc cagccgtact
     3841 gtaccgtttg tcagcaaagc taccggtcgc ccactggcaa aaatcgctgc tcgcgtgatg
     3901 gctggtgaaa cccttgccga cttaaatgca ttagaggaaa ttatcccaag caaattcttc
     3961 gttaaagaag cggtattccc attcatcaaa ttcccgggtg tggataccat tctcgggcct
     4021 gaaatgcgtt ctaccggcga agtgatgggc ataggtaata cctttgctga agcgttcttc
     4081 aaagcacaac tgggtgccgg tgaacgaatt ccaaaagtcg gcaaagtgtt tatgtcggtt
     4141 attgaagagc ataaacctgc tttaatttac attgccaaac gtttccaaga acaaggctac
     4201 ggtttatgtg ccaccttcgg cactgcaaac ttcctacggc aacacggaat tgcagtccaa
     4261 acggtaaaca aagttcgtga agggcgaccg catattgtcg atgcgatcaa aaacggcgaa
     4321 attgcgttgg taatcaacac cgtcagcggc gagcctgaag tgattgccga cagccactct
     4381 atccgccgta ccgccttgca acaacgtgtg cctatttaca ccacccttgc aggtgctgat
     4441 gctatctcct acggcgtaaa acatatcaat gagtttgatg tgtattcagt gcaggaatta
     4501 gaacaaaagg ctaactaaaa taaagctaaa atgacaagcg gtcagatttt gcaaattttt
     4561 tacaaattct gaccgcttgt gtttgtagag gattatttag taaatttata tgctaaatca
     4621 ttccagcgta attcattttt gagttgtggt agctcggtat tttggttgat atggataaac
     4681 tcaataccaa aatattcagc gaacattcgt agcatatcgg catcaatatc taaactaaat
     4741 acactatgat gagcaccgcc ggctagaatc catgcttgag tacctacata aaaatttggt
     4801 tgaagcttcc aaaaagcatg cccaactggt aaatttggca ttggatttgg tgaggcgata
     4861 gcgtctaaat ccgcaacaac cattctaaaa cggtctccca tatctacaac ggttgcattc
     4921 actgcttttc ccgccttact actgaaaatt aagcgaacag gatctgcctt accgccaata
     4981 cctagcggtc gaatggctaa tgttgatttc tctttggcaa tagacggaca aacttcaagc
     5041 atatgagccc ccaataccac ttggttttgt tcatctaaat cataggtgta gtcttccata
     5101 aaggaagaac cgtttggtaa gccataaccc atcactttca cttcacgcac aagagctgcg
     5161 gttttccaac ctccttcacc accaaatccg tagccttgtt ccattaaacg ctgaacagcc
     5221 aagcccggta attgtttaat cccatttaga ttttggaaat tatcagtaaa agctttaaat
     5281 ccaccattat ctaagaatgt ttttaagccg atttcaatac gagcagaata tgcgagagat
     5341 tctcttgctg aaccattttc ttgcaagcta tcctcaacat tatacaagcg gtcagatttt
     5401 gcaaaaaaat gaccgcttgt tgccatttct aaagtgtgat ctacctctca aatttacaaa
     5461 attttttata acatattgat ttaaaatgtt ttttatattt ttcctttaat tgactttaat
     5521 ttgtcttaag agtaacttat aaaacgtgat tttactattt gaaatattga ggtattcaag
     5581 atgactattc gagtttccta tgaagaatta aaagctcaat ttaaacgtgt tctgttggct
     5641 cgcaatgtgc gtgaagagat tgccgaagag tgtgccactg tgtttgccga caccactcaa
     5701 gcgggtgctt attcacacgg ggtaaaccgt ttcccacgct ttattcagca gttagagaac
     5761 ggtgatattg tgccggaagc cgtgccgact aaagtgcttt cactgggggc gattgagcag
     5821 tgggacgctc accaagccat cggtaactta accgcaaaaa aaatgatgga cagagcaatg
     5881 gagcttgcat ctgaaaacgg catcggcgta gtggcgttac gtaatgcaaa ccactggatg
     5941 cgtggcggtt cttacggatg gcaggcggca gagaaaggct atatcgggat ctgctggacg
     6001 aacgcccttg cggtaatgcc gccttggggg gcgaaagagt gccgtatcgg gactaacccg
     6061 ttaatcattg cggtgccgac taacccaatc acgatggtgg atatgtcttg ctcaatgtac
     6121 tcatacggaa tgttggaagt gcatcgcctt gctggtcgtc aaacctttgt cgatgctggt
     6181 tttgatgatg acggcaaccc aacccgtgat ccggcaacgg tcgagaaaaa cctccgtcta
     6241 atgccgatgg gcttctggaa aggttcgggc ttatcgattg tgttagatat gatcgccacc
     6301 ttgctctcta acggtgaatc cactgcggca gtcaccgaag ataaagacga tgaatattgc
     6361 gtgtcgcaag tttatatcgc tattgaagtg gatcgcttaa ttgacggcaa aaccaaagac
     6421 gaaaaactca accgtattat ggaatatgtg aaaaccgcag aacgtgccga ccctgatgtg
     6481 gcagtacgtt taccgggcca cgagttcacc caaattctag ccgataacaa agccaacggc
     6541 attccggtgg atgacaccgt ttgggcgaaa ttacaggcgt tgtaattgca aattgaccgg
     6601 tagggtgggc gtaagcccac gcattagaca caatcataag atgacgtggg cttacgccca
     6661 ccctacaagc ggtcattttt ccgcagaatt ttacaaaacg gaggtatgaa tgttttttgg
     6721 tcatatttca caagttaaag cgaatcgata cccgccggcg atccaaattg cgttgaacta
     6781 tctgaaaaat accgattttg atgcgatgga agcaggggtt tacgaactca aaggcagaca
     6841 aatttatgtg caggtgttgg atttgaacac caaatcgaaa cacgaatttc agccggaagt
     6901 gcatcgcaat tacctcgatg tgcagtatct acaccgaggc aaagagataa tggctgcggc
     6961 ggtggatacc ggcaccaatc cgattgcgat ggaatacaac cctgagcggg atattcaata
     7021 ttatcaatcc gttgccaacg aaaatgaatt tcgctgcgtt gaaggcaatt tttgacggca
     7081 cggaaaccat tcgcaaagtg gtggtaaaaa ttgcgatgag cgaaattgag gagcaacgat
     7141 gaaagcctta gctcaattag tcggaaaact gttagaaatc ttggtggtgg tgattttatc
     7201 cgcaatgtcg attttggttt tcgtcaatgt ggtactacgt tacggtttta acagcagcat
     7261 tagcgtgacc gaagaggtgt ctcgctatat gtttgtttgg ttggcgtttt taggggcggt
     7321 gttggcattt agcgaaaatc aacacgttaa agtcacaatg ctgaccgata aattatcgcc
     7381 aacggctcgt aatatcttaa gcgtagtcac agatttagcg atgttgttct gttgctactt
     7441 aattgtggac ggtagctgga ttcaatttaa cctcaacctg aataactttg ccccgatttc
     7501 aggcttaccg caaggcatca ctttccttgc cagcacagtg gcaggggtat taatcggttt
     7561 attaattatt gcccgcattg tggcaacggt tggcgtgatt gcaaaaggag aaaaatagat
     7621 gactgtggta atttttcttt ccgtcttgct tggctcgatt ttattgggca ttccggtagc
     7681 tttcgccctg ttggtctgcg gtgtggcgtt aatgttgcat ttagatctgt tcgatgccca
     7741 aattttggct caacaaattg tgagcggtgc agacagtttc tcgctaatgg caattccgtt
     7801 cttcattctt gccggtgaaa ttatgaacga gggcggtctc tccaaacgga ttatcgacct
     7861 gccgatgaaa ttagtcggtc ataaacgggg cggtttaggt tttgtagcaa ttttagcggc
     7921 gatgattatg gcaagccttt ccggttcggc ggtggcggat accgcagcgg tagtcgcaat
     7981 gctactgccg atgatgaaaa ccaccggtta tccgattgac cgctctgccg gtttaatcgg
     8041 tacggcaggg attatcgccc caatcattcc gccaagcatt ccgtttatcg tatttggtgt
     8101 ggctagtggc gtgtccatta ccaaactgtt cttagccggt attttcccgg gcattatgat
     8161 ggggatctgc ttggctgccc tttggtggtg gcaggcaaaa cgtttggatt taatgacctt
     8221 ctccaaagcg accaaagaag aactttgcat ttcatttaaa aacagtattt gggcgttatt
     8281 gttgcctgtc attattatcg gtggtttccg ctcgggaatg ttcaccccaa ccgaagcggg
     8341 tgcggtcgca actttctatg ccttgattgt ttcactgttt gtttaccgtg aaatgaagtt
     8401 caaagattta tacaaggtag tgttggcggc agcgaaaacc acggcagtag tgatgttctt
     8461 agtggcagcg gcaaatgtga ccggctggct gattaccgta gcggaattac cgatgatgct
     8521 caccgagctg ttagagccgt taattgaaag cccgaccaca ctcttattgg tgattatgtt
     8581 agcggtgttt attatcggaa tggtgatgga cttaacccca accgtgctga ttttaacgcc
     8641 ggtattaatg ccgttggtgg aagaggcagg cattgacccg gtttacttcg gtgtgctgtt
     8701 tattcttaac acctccatcg gcttgattac accaccggtc ggcaacgtgc taaacgtgat
     8761 taccggcgtg tcgaaactgc cgttcgacca agcggctaag ggcgtaatgc cttacctcgt
     8821 aatgatgatt tgtttactac tgttgttcgt gttcttcccg tcactggtgt tagtgccgtt
     8881 aagctggatg cagtaatggt ttgaaccgaa acaagcggtc gaattgacgc aaaaatttgc
     8941 aaattttttt ggcaaaacag accgcttgca actcaatttt taatcacagg agaaccctat
     9001 gaaattattt aatttaaaaa ccttatttgc cttagtggca ggcgttgcgg taatgagcgg
     9061 ctctgcaatg gcagaaacct cactgcgttt tggttatgaa gccccacgtt ccgataccca
     9121 acacatcgcc gccaaaaaat tcgatgaatt attggcagca aaaaccaaag gtgaaattaa
     9181 attaaaactg tttccggaca gcaccttagg taatgctcaa acgatgatca gcggcgtgcg
     9241 tggcggtact atcgacattg aaatgtccgg ctcgccaaac tttaccggct tagagccaaa
     9301 actaaatgtg attgatattc cgtttatttt caaagatcgt gaacacgttt acaaagtatt
     9361 agacggcgaa gttggtcaag gcttattaaa agatttagaa gcccaaggct taaaaggctt
     9421 agccttctgg gacgttggct tccgtgcttt ctccaactca aaacaccctg tgaccaagcc
     9481 ggaagacatc aaaggcttaa aagtccgcac caaccaaaac ccgatgtaca ttcaagcctt
     9541 caccttacta ggcggcaacc cggtgccaat gccactttct gagctttaca ccgcactcga
     9601 aacccgtgcc gttgatgccc aagaacaccc aatcggcatt ttctggtcgg caaaactgtt
     9661 tgaagtacaa aaacatttaa gtttaaccaa ccacggctac accccgttaa tcgtggtgat
     9721 gaacaaagcg aagtttgacg gcttagctcc tgaattacaa accgcattag ttgaagccgc
     9781 aaaagaagcc ggtcaattcc aacgtgatct caacgtgaaa aacgaaaaag agatcatcga
     9841 aaaattacgc aaacaaggca tcgaagtgat cgaacaagtg gataacgccc cgttcaaagc
     9901 agcgattgaa gcggaagtcc gcaaagcttt cgtggaaaaa cacggcgatg aattattgaa
     9961 aaaagtggat gcgttagcgc agtaattgac tttattttag tctaaagctc cctccgtttg
    10021 gggggagttg ttgtatagac tctactatag gagagactat gaaactgttt aatttaaaaa
    10081 ctttagccgc tttcgttgcc ggttttgcac tgttttcaac cgcacaagca gaaacctcat
    10141 tccgctttgc gtatgaagct cctcgttctg atactcaaca tattgcagcg aaaaaattta
    10201 atgatttgct gcaagaaaaa tcccaaaaag ggctgaaatt aagcctcttc cctgatagca
    10261 cgctcggcaa cgcacaaacg gcgattagcg gtgtgcgtgg cggtactatt gatattgcaa
    10321 tgtccagctc ctctaacttt accgggttag aaccaaaact gaatgtgatt gatattccgt
    10381 ttattttcaa agacagagac cacgcctata aagccttaga tggagagatt ggtcaaggtt
    10441 tattaaccgc attagaggct cacggtttga aagggttggc gttttgggaa gttggtttcc
    10501 gagcgttttc caactccaag cacccgatca ccaaaccgga ggatattaaa ggcttaaaaa
    10561 tccgtactaa ccaaaacccg atgtatattc aagccttctc tattttaggt gcaaatccgg
    10621 tgccaatgcc gctttccaag ctttacactg cactggaaac caaagcggtt gatgcccaag
    10681 aacacccaat cggcattttc tggtcggcaa aactctatga agtacaaaaa tatttcagct
    10741 ttactaacca cggttacacc ccgttaattg tggcgattaa caaagcgaaa tttgatgcac
    10801 tgccagccga gcaacaaacc gcattagttg aagcggcaaa agaagccgga aaataccaac
    10861 gccaacttaa cctagaaaat gagcagaaaa ttatcgactc gctcaaaaaa gcaggtattg
    10921 aatttgtgga taacttagat accacaccgt tcaaagatgc cgtatcggct gaaacccgca
    10981 aggcatttat cgagaaaaac ggtgatggct tagttaaagc cattgatgca ttggcgaagt
    11041 aaaaatagag ctacaaagtg ctcggttaaa ctaacctcac acgaatcgtg tgaggtacaa
    11101 gcggtcaaaa tccgcaaaat ttttacacat ctttcctcaa aggaggaaca aatggcacag
    11161 cccattttaa gtatgcagca catcatcaaa cgctttggta gccatacggc ggtgaatgat
    11221 gtcagccttg atatccactc aggcgagatc gttgccttac tcggtgaaaa cggtgcaggt
    11281 aaatcgaccc taatcaaaat ccttgccggt atttatgacc gtgacgaggg tgatattctt
    11341 ttccataacc aaaaaatcca atctgcccac gctattgaga aaggcaacaa aaagcccatc
    11401 gcttttatcc accaagattt aggcctaata gaatggatga cgattgccga aaatatggca
    11461 tttgtaatgg ggttccctcg caaatttggc ttgattgact gggcgaaagt gcgagagcag
    11521 tcgcaaaaag cccttgattt tgtaggcatt aaactctctg ccgacactcg agtgtttgat
    11581 ttaagccgta ccgaaaaagc cttactcgcc attgcccgtg cgattgccgt caatgcagaa
    11641 atcttggttt tagacgaacc tactgcttcc ctacctgcct ctgatgtaga gcatcttttc
    11701 actgtgttaa accgcttacg ttcccaaggt gtgggaatga tttatgtgac ccaccgttta
    11761 gatgaagtga tcgccatttc cgaccgcatt ttggtgatgc gggacggttt cccggttgca
    11821 gaagggcaaa ccaagctcta tgacgtcaaa ggcttggtca aagcgattgt aggcgaagag
    11881 agccgtggca gacagcgggt ggatttacca accgatactc ctgaaatttt aagcctgcaa
    11941 aatgtcgtgg ttggcgacac cggcccggtg aatttccgtc tgcacaaagg tgagatgatt
    12001 gcccttgccg gcttgcgtgg agcagggcag gaagaaatcg gccgcttatt atttggggag
    12061 cgaaccttag acaaaggcga aattcagtta gacaagcggt catattctgc taaagatccg
    12121 caagatgcga tagccaaagg tgtggcattg gttgcgggag accgcacccg tgaaagcgtg
    12181 tttatgtcaa tgactaccag tgagaacctg tttatcaacc cggtactaag cggtcattca
    12241 ccattaaaac gctactcccg ccgtgaagag tggggtgaga gctggtacaa attccaacta
    12301 ttcgatattc gtccgaaaaa tttgtttatt gatgccagtg cgttatccgg tggtaatcag
    12361 cagaaaatcg tgttggcacg ttggatgcac ctcaatacgc ctgttctgat tttagaagac
    12421 ccgactgccg gcgtggatgt aggagcaagg gcagagattt acgacttgct caacaaagcc
    12481 cttgcagaag gggtcgcaat tattgtgatt tccaccgact ttgaagagat cgctcatttg
    12541 tgtaaccgag cattagtctt caaccgaggg gaaattgccg gtgaactact gaacgaagag
    12601 gtgactttcg ccaatttgct ggcgttggca tcggatagtc agtcgaaaaa acaataactt
    12661 actctcgccc gcttgcggga gagagacagc taaaaatagc gtcaggctat ttttagcaga
    12721 gagagggtaa acaagcggtc atttttctta aaaaatattg cgaaccctct ccctccgaaa
    12781 gcctaacggc tttctcctcc ctctcccgca agcgggcgag gggaaaatgc agaattttaa
    12841 cggagtaatt tatggctcaa acaaacatca aatcaaccgc actggaacaa ccggtatcta
    12901 tcgcccgtga cggttttatg gcatggtttt cacaaatgtc cactcgttat ggcttactgt
    12961 ggttatgtgt gttattggtg attattttct cacttaccac cgaatctttc gcctcaatgc
    13021 tgacattaaa tgcgatttta gaaagtaaat cgaaaattgc cctacttgct ttggcagcca
    13081 caactacaat gattgtcggc aaaatcgacc tgaacgtggg tttcggtatt gtgttgtggc
    13141 acattcttgt gattactctg caagtccaat acggcttctc gtggtatatg gcgatcttaa
    13201 tcgtgttagt gattgccgct ctttacggct tgctgaatgg gattttagtt gctcttgctg
    13261 atatcgatag ctttgtggca acacttggct caggcacagt gctttatgcc gtagcactct
    13321 ggcactcggg cggtcgccaa attgtcgggg atttacccga tgaatttatc gcactcaaca
    13381 gcacagaaat tttcggtatc ccgatttctg ctttctatgt gctggcagtg gctatcgtaa
    13441 tgtggttggt gacagaacat accccgaccg gtcgttgtat gtatgcggtt ggtggcaacc
    13501 caacggcagc tcgcctaaat ggtatcaata ttaaaaaata caccattgtg ccgtttattg
    13561 tctctagtgt aatcactgct ttcacaggcg tattgatctc tgctcaacaa ggcgttggtc
    13621 aggcaagtgt cggtatggac tacttactgc ccgcattggt tggggcattc ttagggagta
    13681 ccactattcg cccagggcgt attaacgtat ggggaacagt ggtggggatt gccattcttg
    13741 ccatcggtat ttcaggtatt caacaattcg gtggagcgtt ctgggttgag ccactcttta
    13801 acggtgcaac actgttactt tccatcacta ttgcaggcta tgcccaacgc aaacgcttgt
    13861 taaatcaaaa agctgtacag aaaaaatcga ccaactaaga ggatttgatt atgaaaaaag
    13921 catttcgtac aaccttaact gcaatcgcaa ccttaactgc tattgctttc ggaaataatg
    13981 cgtttgccaa tgacgacccg ttcgtacaag aagcaaaaca gcttgtcgca gcagctacgg
    14041 ctaagcaaga aaaatgggac ggaccgacaa ccggccctca attacaaaaa gagaaaactg
    14101 tgatttttat tgcctctgat atgaaaaacg gtggtgtact tggtgtgatt gacggaatga
    14161 aagaagctgt tggcgtaacg ggctggaagt tagatgtatt agacggtgca ggtacggtaa
    14221 acaaccaact cgccgcactt aaccaagcga ttgcccgcaa accggatgcg attgtgattg
    14281 gtggctggaa cccgaatgtg gcgaaaatcc cactacaaaa agcgactaaa aacggcatta
    14341 aacttgcagc ttggcacgca acaccggagc cgggtgcgat tgataaatac ggcattttct
    14401 acaacgtgac ctctgactca gacgaaattg cacgcttatc ggcaatgctt gcggtggcag
    14461 aatctaacgg taaggcaaaa gcgattattt taaccgactc gctatatgaa attgcgttac
    14521 gcaaagcgaa cgtcatgaaa gaagttatcg aaaaatgtaa agagtgtaag gtgttagagt
    14581 ttatcgacac gccattggcg gatacctcaa gccgtatgcc taacctcact ttcagcttac
    14641 tgcaaaaata tggcgacgat ttagagtatg cgttggcaat taacgatctc tactttgact
    14701 ttatggctcc gtctttacgc accgctggta aagataaacc gtttagcatt tccgcaggtg
    14761 atggctcagt aacggcttat caacgtattc gcaatggtga taaacagttt gcaaccgtcc
    14821 ctgagccact taacttacac ggctggcagt tagtcgatga gctaaaccgt gctttcgcta
    14881 acgaaaaacc gtctggttat atcacaccgg ctcacctagt gactaaagaa aatgtggcgt
    14941 ttgacggtgg tgaaaacaac ctgtatgccc cacaaaacgg ttacaaaggc gcttacaaag
    15001 cgatttgggg cgtgaagtaa tgacgtaggg gcaaataaat atttgcctcg aacaagcggt
    15061 tgttttttgc aaagattttg cgaaatttaa ccgcttgtca taaaaaatag agagggtaat
    15121 acaaatgagc aataaattta tccccaaaat ctccaaccct cgttttaatg agctggttgg
    15181 gaatacgttt gaacttgaag agctttttga taaggcaatc tgggcagaag ggccggcgtg
    15241 gagcaagcca caacaagccc tctattttag tgatgtaaaa ggttgcacaa tgtaccgctg
    15301 gacagagcaa gatggtacac aggttttccg ccagccgtct tatttttcta acggcaatgc
    15361 gtttgatgca aacgacaact tgattacctg tgaacacggc aatcgaggta tcagtttaac
    15421 cacgccaagc ggtgaatatc gttttttggt cggtcaatta gacggaaaac gactgaactc
    15481 acccaatgat gtggtggtaa aatcagacgg catcatttgg ttcactgatc caccttacgg
    15541 tattttgtcg gataacgaag gcaaacagtc gccaagcgaa attatcggtt gttatattta
    15601 ctgctatgat ccgaaaaccg acaagctgaa tattgcaact ttcaacacga tgcgaccaaa
    15661 cggcttggcc ttctcgtcag acgaaaaaca actctttgtg gcagatatgt cgagcgtgga
    15721 atttgacgaa gatggcttac accacttagt cgtatttgat gtggacggta ataaactaaa
    15781 aaatcgccga gatgtggcgg aaattagccc cggcattccg gacggcttcc gaattaaaca
    15841 aaacggcgta attttctgct cttgtgaaga gggaattatt gctcttttac ctgacggcac
    15901 ggagctggga cgatttgtgc tgggtaagct aacctcaaat tgtagctttg gcgatgatga
    15961 acaaacactg tttattactt gttcgaatag cgtttatcgt ttaacaatta aatagcagaa
    16021 tgcattaagt gcgtctgttg tagcattaca agcggtttct ttttcaaaat tttttacaaa
    16081 caaataatgg ataacaaaat gaactacaca ctcggcattg attgcggtgg gacttttatc
    16141 aaagccgctc tttttgatac acaaggtaat attcaagccc ttcatcgtga aaacgtttcg
    16201 gtaatcagtg aacaggcagg ttatgcagag cgtgatatgt cacaactctg ggctatttgt
    16261 gcggaagtag tgcgttcaac cattgaaaaa agtggggtaa acccagcgga tattaaagca
    16321 gtagggattt ccgctcaagg caaaggggcg tttttactgg ataagcagaa tcgaccgttg
    16381 gggcgtgcga ttttatcttc cgaccaacgt tcccttgaga ttgttaaaca atggcaggct
    16441 gaaggcattc cgcaacaact ttacccgatt acccgccaaa ctctttggac aggacaccct
    16501 gtgtcgattt tgaagtgggt gcagcaaaat gaaccggagc gttatgcaca aatcggggcg
    16561 gtgttgatgt cgcacgacta tttgcgtttt tgcttaaccg gtgagttgca ttgcgaagag
    16621 accaacattt ccgaaagcaa tctttacaat atggaaaaag gcgaatacga ccaacagtta
    16681 gccgatttgt tgggaatgcc ggatgtgttg gagaaattac caccagtgat tgaaccgaat
    16741 aaaatttcgg gatatgtgac tgaaaatgcc gccaaattaa ccggtttagc ggtgggtacg
    16801 ccgatagtcg gtggcttgtt tgatgtggtt tctaccgcac gttgtgccaa tttagatgac
    16861 gaaaccaaac tgaatgcagt gctgggaacg tggtcggtgg tgagcggcat taccgatcat
    16921 attgatgcaa gccaatcatt accttttgtt tatgggcgtt atgccgaggc agggaaattt
    16981 atcgtccacg aagcaagccc gacttctgcc ggtaatttag agtggtttgt gaatcagtgg
    17041 aaactcgatt atgtgaaaat caaccaagct gttgcccaat taccgccggc ggcaagctcg
    17101 gtgctgtttg tgccgtttct gtatggctca aatgcaggct taggaatgca agccggtttc
    17161 tatggtatgc aagcccacca tacccaagcc catctcttgc aggcaattta cgaaggagtg
    17221 ttgtttagct tgatgagcca tttaaaccgt atgtttatcc gctttccaaa ggctgacgtg
    17281 ctgcgagtta ccggcggacc ggtgaaatct aaagtttgga tgcaaatgtt ggccgattta
    17341 accggaatgc ggcttgaaat accacaaatg gaagaaaccg gctgcttggg tgcggcatta
    17401 atggcaatgc aaggggttgg gctggatata agccaagttc aagccctaaa taatgaaatg
    17461 caggtgtttg agccaaatcc ggaaaattat gcggcttatc agacgaaata tcagcgttac
    17521 caaaaattaa ctgaagcatt aaatacaatg atttaacctg aatttatgct tttacaagcg
    17581 gtaatatttg gttacttttt tgcaaaaa
//
LOCUS       DANLZC010000041        17272 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.041, whole genome shotgun sequence.
ACCESSION   DANLZC010000041 DANLZC010000000
VERSION     DANLZC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17272
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.041"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(760..1275)
                     /locus_tag="PXE44_001053"
     CDS             complement(760..1275)
                     /locus_tag="PXE44_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5588574.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GERREVTEWHRIVFYRRQAEVCGQYLRKGSQVYVEGRLKTRKWQDQNGQDRYTTEIQG
                     DVLQMLGSRQGNDMGGQQGGWGNSAPAGNSYNQGNSNYGYNQTQSAAPQSKPAPQPEP
                     AMNNFDDDIPF"
     gene            1430..4258
                     /gene="uvrA"
                     /locus_tag="PXE44_001054"
     CDS             1430..4258
                     /gene="uvrA"
                     /locus_tag="PXE44_001054"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL5588575.1"
                     /translation="MQHIDIRGARTHNLKNINLTLPRDKFIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTISQMVDRVLEEPEGKRLMLLAPVVKERKG
                     EHVKLLENLTASGYLRARIDGEICDLSDPPALELQKKHTIEVVVDRFKVRADLATRLA
                     ESFETALDLSGSTAIIADMDDPNAEEIVFSSSFACPHCGYSLHELEPRLFSFNNPAGA
                     CPTCDGLGVEQFFDAKKVVQNEEVSLAAGAIKGWDRRSFYYFGLLKAVAEHYGFDLET
                     PFGQLPKKYQNIILNGTKEEIEFVYVNDRGDKVKRKHAFEGVLNNMARRYKETESNSV
                     REELAKYINNRPCMDCEGSRLRREARYVFLDKTNLPMVSEKSIGEALTFFEELKLAGQ
                     KEKIAEKILKEIRERLQFLVNVGLNYLSLSRSAETLSGGEAQRIRLASQIGAGLVGVM
                     YVLDEPSIGLHQRDNERLLNTLVHLRNLGNTVIVVEHDEDAIRAADHIVDIGPGAGVH
                     GGQVIAQGTAAEIMQIEESITGQFLSGKQKIEIPAQRVPYEPTKLLTLSGASGNNLKK
                     VKLEIPVGLFTCITGVSGSGKSTLVNDTLFPLAQNALNRAENSDVAPYESIDGLSHFD
                     KVIDIDQSPIGRTPRSNPATYTGLFTPIREIFAGTQEARARGYNVGRFSFNVRGGRCE
                     ACQGDGVIKVEMHFLPDVYVPCDHCKGKRYNRETLEIRYKGKTIHQVLEMTVEEAREF
                     FAPMPMIARKLQTLMDVGLSYIRLGQSSTTLSGGEAQRVKLATELSKRDTGKTLYILD
                     EPTTGLHFADIKQLLGVLHRLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGEI
                     IATGTPEEVAKDKRSHTARFLKEILAKG"
     gene            complement(4350..6083)
                     /gene="dauA"
                     /locus_tag="PXE44_001055"
     CDS             complement(4350..6083)
                     /gene="dauA"
                     /locus_tag="PXE44_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDL5588576.1"
                     /translation="MLNKWFLTKNAFLAVKPFSALKDALNAGYGKQHLIRDIISGLTV
                     GVIAIPLSMALAIASGVPPQHGLYTAIVAGIVIALTGGSRFNISGPTAAFVVILYPVT
                     QQFGLSGLLMATMLSGGILVLMALLRLGRLIEYIPLPVTLGFTCGIGIVIATLQIKDF
                     LGLSIEKMPEHYLGKLEAIFSALPTINWADTLVGVVTLLVLTRWHKLRLPIPGHLPAV
                     FIGTLLSLLLIHFGQNVATIGSAFHYTLSDGTTGYGIPSVLPEFVLPWNLPNAEGNRI
                     DWNLTTIQALLPAAFSMAMLGAIESLLCAVVCDNMTDTKHHSNNELLAQGLGNIASPF
                     LGGITATAAIARSAANVRSGAVSPISSVVHALLVLFALLFLAKALSYLPLSSMAALLL
                     VVAWNMADIPQIIQLTRRSGKNEIAVLLTCITLTVLFDMVIAITVGVLLASLLFIRTI
                     AEMTKSFEMAHPTDLNDVLVYRISGPLFFAAADNLFADLHNKTVHTDHQIKHIVLQCD
                     AVTVLDAGGIHALTRFVQHMLPHQQLHLCNVQFQPMRMLVRSQKTVNEIPQIQFSSDL
                     ESAFDKIRQAN"
     gene            6325..7257
                     /locus_tag="PXE44_001056"
     CDS             6325..7257
                     /locus_tag="PXE44_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5588577.1"
                     /translation="MDSLIHFLSAFLTNITWIGALELGLIYSLVALGVLISYKILDFP
                     DLTADGSFPLGGAVCVISILNQVDPWLATLYGTLAGAVAGMVTASLHIGFKIEKLLAS
                     ILMMIALYSINLRIMGMPNIAILGETTIYDVMPIESDLQLALVRLVMALLVVIIVKGL
                     FDLFFATEIGLAVRATGTNSRMAKAQGIAINKMTILGMAISNALIALGGALYVQSNGG
                     VDISIGVGTIVIGLAAVIIGEAIFSVKRIIWLTFAVIIGSILYRFFIALALNNETLNG
                     IGFGPQDLNLITALLVVIVLAVPRIKHKLLGKRG"
     gene            7259..8056
                     /locus_tag="PXE44_001057"
     CDS             7259..8056
                     /locus_tag="PXE44_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5588578.1"
                     /translation="MIELNNLFITFNKGTAIENPVLRGLSLKVEQGEFVSVIGSNGAG
                     KSTLLNAISGDCAVDSGEILIQGNKVNQSTTWQRANQVARVFQDPMAGTCESLTIEEN
                     MALAYQRGRKRGLGFAIKRQMRELFKDKLALLGLGLENRLTDQMGRLSGGQRQAVSLL
                     MASLQPSNILLLDEHTAALDPKTTDFVLELTNKIVREQKLTTLMVTHSMRQALDYGDR
                     TVMLHQGQVAFDVSGEQRQKMDVPDLLQLFQQSRHEQLSDDGLLLGN"
     gene            8143..8364
                     /locus_tag="PXE44_001058"
     CDS             8143..8364
                     /locus_tag="PXE44_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588579.1"
                     /translation="MLSIFKTPVEKETLDDWAKISVDVAKVAILAIPVILFGKEPTLI
                     KLTNLALLVLSIYVFLTLGRKLRQLKEVL"
     gene            8547..10472
                     /locus_tag="PXE44_001059"
     CDS             8547..10472
                     /locus_tag="PXE44_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL5588580.1"
                     /translation="MTKYDDKIQHAFSENGLLSQNISGFRPRQAQLEMAQVVAKAVKF
                     ATPVVVEAGTGTGKTFAYLVPALLSGKKTILSTGSKNLQDQLFNRDLPTIQKALKYKG
                     KIALLKGRANYLCLERLDQVTAMGVLGDKSVLTDLKKVSKWQNATKTGDLSECITIAE
                     DSPILPQLVSTTESCLGSDCPHFKDCYVVQARRKAMEADVVVVNHHLFCADMAVKKTG
                     FGELIPEAELVIFDEAHQLPDIASQYFGQSLSSRQLFDICKDTNIVYRTELKDLSQLG
                     KAADHLTKVVQDFRLLMGGEGQIRGNLRELFRDKKVVDGLVKVAENIDFLSEVAKKSL
                     GRSETLDKIFERLAEVKVQLKKLMDTSVTGYCYWYEANGRSFGLHITPLMVADKFGEQ
                     LKNQQIGWVFTSATLEVGGKFDHFCQRLGIENAEQLVLQSPFDYQHQSLLCVPRYLPD
                     TNKSNTLTALGQLLKPVIEANKGRCFLLCTSYYMMRGLADFLREHSSLNVLLQGETSK
                     SRLLEKFVKEDHSVLVATQSFWEGIDVRGDDLSLVIIDKLPFTSPDEPLLKARMEDCK
                     IQGGDPFNDIQIPEAVITLKQGVGRLIRDVTDRGVVIICDSRLVMRNYGQTFLKSLPP
                     SRRTRDLGNVIQFLKQK"
     gene            10546..10911
                     /locus_tag="PXE44_001060"
     CDS             10546..10911
                     /locus_tag="PXE44_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL5588581.1"
                     /translation="MLSTLLRMVASLALLFVMLYLGKLIVYLFPIGIPDSILGMLILF
                     GCLVIGIVKVEWVIPSAKPLTRYITLFFLPICVELIEHLELLRQNLNTFVLATALSTL
                     VSVVAIGVFAQWIFHYKRG"
     gene            10913..11599
                     /locus_tag="PXE44_001061"
     CDS             10913..11599
                     /locus_tag="PXE44_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL5588582.1"
                     /translation="MSYLYTVLTVIMFIAGLKISQKLKSSFLNPFLIALALLIGVLLI
                     FNIPYQDYYQGNSPLNNLLGVSVVALAYPFYEQLPHIRKKWRQIVVILLFSTIITMIT
                     GLGFSLLFGASQEILAAILPKSVSMPMAIIITNEINGNVAIAAVGVMIAGLVGSILGF
                     TLLRLAKVRNVMVIGLSMGAASHALGTARCLEHSMKAGSYSSIALVACGVLSSIFAPL
                     IFNVIVTLCY"
     gene            11683..13446
                     /gene="msbA"
                     /locus_tag="PXE44_001062"
     CDS             11683..13446
                     /gene="msbA"
                     /locus_tag="PXE44_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDL5588583.1"
                     /translation="MQEQITQEKDLSTLKTFKRLWPTISPFKAGLITGAVALVLNALV
                     DSSLIYLLKPLLDEGFGQANNDFLKLMAVLVVVFILVRGITNYIASYCLAWVSGKVVM
                     TLRRHIFQHLMYMPVSYFDNNPTGRLLSRVTYDTEMVASSASHSLVTIVREGAYLLSL
                     FVVMIYTSWQLSIVLFVMAPIIGVLISFVSKYFRQLSRNIQSSMGELTVTTEQMLKGH
                     KVVLSFGGQEIEKARFDRVSNDMRRKGMKVVSADGISDGVVQLIASFALSAVLYVATF
                     PEVMSENLTAGSFTVVFSSMMAMLRPLKSLTNVNSQFQRGMAACQTLFEFLDLKTEKD
                     HGLISVAKAEGNVEFKKVSFSYEGKEEKALDNISFTIPKGKTVALVGRSGSGKSTIAN
                     LLTRFYDVTDGQILLDGINIQDYRLKNLREQCSVVSQQVHLFNDTIANNIAYAAIDKY
                     SREEIIAAAKAAHAMEFIEKLENGLDTVIGENGASLSGGQRQRLAIARALLRNSPVLV
                     LDEATSALDTESERAIQSALEVLQKDRTVLVIAHRLSTIEKADEILVVDHGNIIERGS
                     HTELLAQGGAYKQLHSLQFGA"
     gene            13535..14167
                     /gene="lolB"
                     /locus_tag="PXE44_001063"
     CDS             13535..14167
                     /gene="lolB"
                     /locus_tag="PXE44_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDL5588584.1"
                     /translation="MKKLTKLLSLSALLVLTACNSVLNASTEVQQPTVQIPHDDPQWQ
                     QHLAKLSQIQGYTATGQFGYISPEERFSSHFNWRYTNPIQFGLELSSNLSTKSLKLER
                     NKRGLTVSDNEGNSRSDRDVDALMQEIIGVSFPIDQFAYWVKGLPEKDGNYIVNEKRQ
                     LSQFSYPLNGTLWKASYVEYHEDRVPNLPKLIVLENGTQTLKIRIEKWAY"
     gene            14155..15009
                     /gene="ispE"
                     /locus_tag="PXE44_001064"
     CDS             14155..15009
                     /gene="ispE"
                     /locus_tag="PXE44_001064"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL5588585.1"
                     /translation="MGVLMKKVTLPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGD
                     EIEIEITESSEVELINPVEGVATEENLIYRAAKLLQKTTACRQGCRIGIKKNLPMGGG
                     VGGGSSNAATVLVGLNHLWNTELSLEKLAELGLSLGADVPIFVRGMAAFAEGVGEVLT
                     PCDPQEKWYVVLKPEVSISTAAVFTDPNLPRNTPKRPLAELLSLPWENDCEKVVREKY
                     SAVEDLIQELLQYAQFRLTGTGACIFAEFETKAEAEQVFAHKPEAVFGFVAKGQNISP
                     LHQMLFQI"
     gene            15035..15985
                     /locus_tag="PXE44_001065"
     CDS             15035..15985
                     /locus_tag="PXE44_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDL5588586.1"
                     /translation="MTDIKLFAGNATPELANRIAKHLYTSLGNATVGRFSDGEIQVQI
                     NENVRGGDIFIVQSTCAPTNDNLMELIVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVIADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPVLIDDILKKS
                     DLVNPIVVSPDIGGVVRARAVAKLLNDTDMAIIDKRRPKANVSQVMHIIGDVADRDCI
                     LVDDMIDTGGTLVKAAEALKERGARRVFAYATHAVFSGTAAQNLANPALDEIVVTDTI
                     PLSNEIKALNKVRVLTLSGMLAEAIRRISNEESISAMFDA"
     gene            16063..17073
                     /gene="pyrD"
                     /locus_tag="PXE44_001066"
     CDS             16063..17073
                     /gene="pyrD"
                     /locus_tag="PXE44_001066"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDL5588587.1"
                     /translation="MYSLIRKLLFALDTEKAHQFSIQALALAGKSPFSILPTPDNPTQ
                     VMGLTFKNPIGLAAGADKNGEAIDGFGKLGFGFIEVGTITPLAQDGNPKPRQFRIVDA
                     EGIINRNGFNNKGVDVLVENVKNATYSGILGINIGKNAVTPIEHSFDDYQICLRKVYQ
                     YADYVAVNISSPNTKNLRSLQYGEALDDLLRNLKNEQAQLSQKMGKYRPLVLKIAPDL
                     TDEEIVSVADSLIRYQIDGVIAGNTTLSRESIAGLPYSEQLGGLSGKPLHSLSTRLIS
                     KLSAELGERIPIIGCGGVHSLESGQEKVNAGASLLQVYSAMIYQGPSLIQTLAKHLQL
                     NS"
BASE COUNT         5003 a         3252 c         3939 g         5078 t
ORIGIN      
        1 tgtctgccac cgctaggcct ttacatccta gattagtatc agagttttct ggtgcctgcc
       61 accgctaggc ctttacatcc tagattaata tcagattgac gatgagccaa caactcattg
      121 cgttattaag gtaacgccac cttgacaatc agattataat gattatctcc taaagtcaag
      181 ctagtatata ttatatttgt aatctaacct gtaaaaaata tactataaaa tcattatcag
      241 gctttacaaa cttgaaagaa tgtgtaagat caggatcgtc tggaattcgg acagaaaatc
      301 ctagtggatg tattggctgg atacatcgta gtaagtgtgt atttgcgaat attatttgaa
      361 cagttctacg cacttgattc aaaatccgcc gcccataaag cgtgtcggtt cgagtccgac
      421 cactggcacc actctaatat cttttcttat ttactattaa gaatttctct attatcttcc
      481 tatatcaatt tttctcaaaa tattgtattt ataaacccgt atagatggga actttagccg
      541 tttatgtgag aaatggggca ataataaatc tgtcaataag ttacttatta atcgaatgaa
      601 aagtggattt attaattata ttaattaata gggaacactt attacttgga agtgttccct
      661 attattttga gtaaggtagg ggcgctttat atgagtagct ttttatttca tcgaaatgcg
      721 gtgtatggaa cccatgagct acttaagtca atactttgat tagaacggaa tatcatcatc
      781 aaaattgttc attgccggct caggctgtgg tgccggttta gattgcggtg cagcactttg
      841 ggtttggttg taaccataat tgctgttgcc ttgattgtag ctattacccg caggagcaga
      901 atttccccaa ccgccttgct gaccgcccat atcattacct tgacggctgc cgagcatttg
      961 tagcacatcg ccttgaattt cggtggtgta acgatcttgc ccgttttggt cttgccattt
     1021 acgggttttt aaacgtcctt ctacgtaaac ttgagagcct ttacgtaaat attgcccgca
     1081 tacttctgct tgacggcggt agaatacaat acgatgccac tcggttactt cacggcgttc
     1141 accggtgttt ttatccgtcc agctttctga ggttgccaca ctaatatttg caaccgcttc
     1201 gccatttggc attgtacgca tttcagggtc gttacctaaa ttaccgacaa taattacttt
     1261 attgatacca gccataaaat cctctgtttg ataaattaaa ctaaaatttg ctaagtattc
     1321 tacctgaaaa tccaaaagat gaacaccaaa aaactgatat tttgtacagt tttattaaaa
     1381 tatgcgataa tgatcgaaaa ttgagaaaat agcactggac attaaaaata tgcaacatat
     1441 cgatattcgt ggagcaagaa cacacaactt aaaaaacatc aatttaacct tgccgagaga
     1501 taaattcatt gtaattaccg ggctttccgg ctcgggcaaa tcgtctctgg cgtttgatac
     1561 gctctatgcg gaaggacaac gccgttatgt agaatcactg tctgcatacg cccgccagtt
     1621 tctctcctta atggaaaagc ctgatgtgga tcatattgaa gggctttccc ctgcgatttc
     1681 gattgagcaa aaatcaacct ctcacaaccc acgttcgacc gtaggaacgg taacggaaat
     1741 tcacgactat ttacgtttat tgtttgctcg agtgggcgag cctcgttgcc ctgaccacga
     1801 tgtgccattg gctgctcaaa ctatttcgca gatggtggat cgagtattgg aagaaccgga
     1861 aggaaaacgg ttgatgctac ttgcccctgt ggtgaaagag cgtaaaggcg aacacgtaaa
     1921 attattggaa aatttgaccg ctagtggcta tttgcgggct agaattgacg gtgaaatttg
     1981 cgatttatcc gacccaccgg cattagaatt acagaaaaaa cacaccattg aagtggtggt
     2041 ggatcgcttt aaagtaaggg ctgatctagc cacccgtttg gcggaatctt ttgaaacagc
     2101 gttagattta tccggctcta ccgcaattat tgcagatatg gacgacccaa atgccgaaga
     2161 aattgtgttt tcgtccagct ttgcttgtcc gcattgtggt tactctttac acgaattaga
     2221 accacgcctt ttctcgttta acaacccggc aggtgcttgc ccgacttgtg atggtttggg
     2281 cgttgagcag ttttttgatg ccaaaaaagt agtgcaaaat gaagaggttt cattagccgc
     2341 cggtgcgatc aaaggctggg atcgccgcag tttctactat tttggcttat taaaagccgt
     2401 tgccgagcat tatggctttg atttagaaac cccattcggg cagctaccga aaaaatatca
     2461 aaacattatt ttaaacggca ctaaagaaga gattgagttt gtttatgtca atgaccgagg
     2521 cgataaggtc aaacgtaaac acgcttttga aggggtgctg aataatatgg ctcgccgcta
     2581 taaagaaacc gaatcaaact cggtgcgtga agagttggcg aagtatatta ataatcgtcc
     2641 ttgtatggat tgcgaaggct cacggttacg ccgtgaagcc cgttatgtat tcttagataa
     2701 aaccaatttg ccgatggttt cggaaaaaag tatcggcgag gcattaacct tttttgaaga
     2761 gctgaaatta gccggtcaga aagaaaaaat tgctgagaaa atcctcaaag aaattcgtga
     2821 acggctacag tttttagtga atgtcggctt gaattatctt tctctttccc gctcggcaga
     2881 aaccctttca ggtggcgagg ctcagcggat tcgccttgcc agccaaattg gtgcgggttt
     2941 agtaggggta atgtatgtgc tggatgagcc ttccatcggc ttgcaccaac gagacaatga
     3001 acgcctactg aatactttgg tgcatttacg caatttaggc aataccgtga ttgtggttga
     3061 acacgatgaa gatgcgatcc gagccgcaga tcatatcgtg gatataggcc cgggtgccgg
     3121 tgtacacggt gggcaagtta tcgcacaagg tacggcagct gaaattatgc aaattgagga
     3181 atctattacc ggacaattcc tctcaggcaa gcaaaaaatt gagattccgg ctcagcgtgt
     3241 gccttatgag ccaactaaat tgctcacctt atcgggagca agtggtaaca acttgaaaaa
     3301 agtgaaatta gagattccgg ttggtttatt tacttgtatc acgggtgtat caggttcggg
     3361 caaatcaact ttagttaatg acacactatt ccctctggca cagaatgcgt taaaccgagc
     3421 ggaaaacagc gatgttgctc cttatgaatc gattgacggc ttatcgcatt ttgacaaggt
     3481 aattgatatt gaccaaagcc cgattgggag aactcctcgc tcaaatccgg caacttacac
     3541 cggattattt acgccaattc gtgagatttt tgccggcacg caagaagctc gagccagagg
     3601 gtataacgta gggcggttta gctttaatgt gcggggcgga cgttgcgaag cctgtcaagg
     3661 tgacggcgtg attaaagttg aaatgcactt cttgcctgat gtgtatgtgc cttgcgatca
     3721 ctgtaaaggc aaacgctata accgtgaaac cttggaaatt cgctataaag gcaaaactat
     3781 tcaccaagtg ttagagatga cggtggaaga ggcgagagaa ttttttgccc ctatgccgat
     3841 gattgcccgc aagttgcaaa ccttgatgga cgtggggtta tcttatatcc gtttagggca
     3901 atcttctact acgctctcag gcggtgaagc ccaacgggtg aaattggcga ccgagctttc
     3961 caaacgagac accggcaaaa cgctctatat tttagatgaa ccgaccaccg gcttgcattt
     4021 tgccgatatt aaacagttac tcggcgtgct acatcgcctg cgtgatcaag gcaatacgat
     4081 tgtggtgatc gaacataatt tagatgtgat taaaaccgca gactggattg tcgatcttgg
     4141 ccctgaaggt ggcagtggcg gcggtgaaat tattgctacc ggcacaccgg aagaagtggc
     4201 aaaagacaaa cgttctcaca ccgcacgctt cttaaaagag attttggcta aaggctaacg
     4261 ttcaacacaa gcggtcatat tttcggaaaa tgttgcaatt tgcaaaaagt tggaaaatat
     4321 gaccgcttgt tttatatccg gagttattcc tagttagcct gtctgatttt gtcgaaggca
     4381 gattcaagat cgctactgaa ttgaatttgc gggatttcgt ttaccgtttt ttgtgagcgg
     4441 actagcattc tcatcggctg aaattgcaca ttacagagat gaagttgttg atgtggcaac
     4501 atatgctgta caaagcgggt tagggcgtga atgccaccag catctaatac tgtaacggca
     4561 tcgcattgca gcacaatatg tttaatttgg tgatcggtat gtacggtttt attgtgcaga
     4621 tcggcaaaga gattatcggc ggcagcaaaa aataacggac cgctgatccg ataaaccaac
     4681 acatcgttca aatcggttgg gtgagccatt tcaaaggatt tagtcatttc tgcaatagta
     4741 cggataaata gcaggcttgc cagtaacacc cctaccgtaa tggcaattac catatcaaat
     4801 aataccgtga gggtaatgca agtcagtaaa accgcaattt catttttacc tgaacggcgg
     4861 gtaagttgaa tgatttgtgg aatatctgcc atattccaag ctactaccaa taatagtgca
     4921 gccattgagg aaagaggcaa ataggaaagt gctttagcca aaaagagtag ggcaaataaa
     4981 accaacaagg cgtgaaccac actagaaatg ggggaaactg ccccggaacg aacattggca
     5041 gccgatctcg caatcgctgc tgtggcggta attcccccta aaaatggcga ggcaatatta
     5101 cctaagcctt gggctaatag ttcgttgtta gagtggtgct tggtatctgt catattatcg
     5161 cacactaccg cacataataa cgattcgata gccccaagca ttgccattga aaaggcagcc
     5221 ggtaataagg cttgaatggt ggttaagttc caatctatcc gattcccctc tgcattcggg
     5281 agattccaag gtaacacaaa ttcaggtaat acgcttggga taccataccc agtggtgccg
     5341 tcagagagcg tgtagtggaa tgctgagccg atagtggcaa cgttttgtcc gaaatgaatc
     5401 agcagtaatg ataacagcgt gccgataaag acggcgggta aatgcccggg aataggcaac
     5461 cgtagcttgt gccaacgagt taaaaccaac aatgttacga cacccactaa ggtgtcagcc
     5521 caatttatgg ttggcaatgc tgaaaaaata gcctctagct tgccaagata gtgttcaggc
     5581 attttttcaa tgcttaagcc cagaaaatct ttgatttgca aggtggcgat cacaataccg
     5641 atgccacagg taaaacccag tgtgacaggt agaggaatgt attcaattaa gcgtcctaat
     5701 cggagtaacg ccatcaatac taaaattcct ccggaaagca ttgttgccat gagtaatcca
     5761 cttaagccaa actgctgcgt gacggggtat agaatcacca caaaagcggc tgttgggcct
     5821 gaaatattaa atcttgaacc gcctgttagg gcaatcacaa tcccggcaac aatcgccgta
     5881 tataaaccgt gctgtggtgg cacaccgctg gcaatcgcca acgccataga taacggaata
     5941 gcaataacgc caacggttaa gcctgaaata atatctctga ttaaatgttg tttaccataa
     6001 cctgcattga gagcatcttt taatgcacta aaaggcttga cggcaagaaa agcattttta
     6061 gttaaaaacc atttatttaa catagaatgt tcgagtgaag agaatgtttc gcttatttta
     6121 ctgcaatgaa taaaattaaa tttgagctaa cgcaagaaaa agcaggaaag acgaaatatg
     6181 ttggagacaa gcggttgttt taggcgaaaa atttgcaaat acaaataatt agctgatttt
     6241 attttgaaaa tagcgtaaaa tgccacttta atttgtacta ctatagtagt gcatttgtgt
     6301 gatttcatta actataataa acaaatggac agtcttattc attttttgag tgccttttta
     6361 actaatatca cttggattgg ggcgttagag ctggggttaa tttattccct tgttgccctt
     6421 ggggtattaa tttcttataa aattctggat tttccggatc tgaccgcaga cggcagtttc
     6481 cctttaggag gggcggtgtg tgtgatcagc attttgaatc aagttgatcc ttggctggca
     6541 actttatacg gcacgcttgc cggagcagta gccggtatgg ttactgcaag tttgcatatc
     6601 ggctttaaaa tcgaaaaatt gcttgccagt attttaatga tgatcgcact ctattcgatt
     6661 aatctacgca ttatgggaat gccgaatatt gctattttag gcgaaaccac gatttacgat
     6721 gtaatgccga ttgaaagcga tctacagtta gccttagtac gattagtgat ggcattgctg
     6781 gttgttatta tcgtaaaagg tttgtttgac ctcttttttg cgaccgaaat cggtttggct
     6841 gtgcgggcaa ccggtactaa tagtcgaatg gcgaaagcac agggtattgc gattaataaa
     6901 atgacgattt tagggatggc gatttctaat gcgttaattg ccttaggtgg tgctttgtat
     6961 gtgcaaagca acggtggtgt agatatttcg attggggtag gaactatcgt gattggcttg
     7021 gcggcggtaa ttatcgggga agcaattttt tcggtaaaac ggattatttg gcttaccttt
     7081 gcggtgatta ttggctcaat tctttatcgt ttcttcatcg cactagcatt aaataacgaa
     7141 actttaaacg gcattggctt tggcccgcaa gatctgaatt taattacggc gttattggtg
     7201 gtgattgtat tagcggttcc tcgaattaaa cacaaattgc tcggtaagcg aggttaatat
     7261 gattgaactc aataacttat ttattacctt caacaaaggg acagcaattg agaaccctgt
     7321 gctgagaggc ttatccctaa aagttgagca aggcgaattt gtgtcggtaa ttggtagcaa
     7381 tggggcgggt aaatctacct tattaaatgc gatcagtggt gattgtgcgg tggactccgg
     7441 tgagatttta attcaaggaa ataaggtaaa ccaatccacc acttggcaac gggcaaatca
     7501 agtcgcccga gttttccaag acccgatggc tggcacttgt gaaagtttaa cgattgaaga
     7561 aaatatggca ctggcttacc aacgtggacg taaacgtggg ttgggctttg cgatcaaacg
     7621 ccaaatgcga gagttattca aagacaaact tgctttgtta gggctaggtt tggaaaatcg
     7681 gcttactgat caaatggggc gtttatcagg cgggcaacgc caagcggtga gcttgctgat
     7741 ggcatcactt cagccgtcta atatcttatt gttagatgaa cacaccgcgg cactggatcc
     7801 gaaaaccacc gattttgtgc ttgaacttac caataaaatt gtaagagaac agaaattaac
     7861 cacgctaatg gtaacgcatt caatgcggca agcccttgac tatggcgata gaaccgtgat
     7921 gttacaccaa gggcaagtgg cgtttgatgt ttcgggcgag caacggcaaa aaatggacgt
     7981 gccggatctg ttacaacttt tccaacaaag tcgccacgag cagttgagtg acgatggatt
     8041 attactcggc aattaatttt gcgatctgca tcgaaaaaat gaatttctcc ttttgttttt
     8101 ggctgtcaaa ttttaaataa taagcccaaa aggaggtatt caatgttaag cattttcaaa
     8161 acaccggtag agaaagaaac gctagacgat tgggctaaaa tttcggttga tgtagcgaaa
     8221 gttgcgattt tggcaattcc tgttatactt tttggcaaag aaccaacgtt gattaaattg
     8281 actaacttag cattattggt attgagtatt tatgtttttt tgacgcttgg gcgaaaatta
     8341 cggcagctaa aggaggtgtt gtgatggaac taggctggaa tttaaccata ggtttgctaa
     8401 ttttaaacgt ctttgcgttt gcttggcttt atattattaa ccggatttct aaagaacaat
     8461 tatccaaaaa tcaataatat ttaatttttc acaagcggtt gtttttgcaa aatattttgt
     8521 aaattcgacc gcttgcctgt ttttaaatga caaaatacga cgataaaatt cagcatgcat
     8581 ttagcgaaaa tgggttgctc agccaaaata tcagtggatt tcgaccacgc caagcacagc
     8641 ttgaaatggc tcaagtggtc gcaaaagcag taaaatttgc gacacctgtt gtggtagagg
     8701 cgggaaccgg cacaggcaaa acctttgctt atcttgtgcc ggcactactt tcaggcaaga
     8761 aaactattct ttcgacaggt tctaaaaacc tgcaagatca actttttaat cgagatcttc
     8821 ccactattca aaaagccctg aaatacaaag gcaaaattgc gttactcaaa gggcgtgcga
     8881 actatttgtg tttagagcgt ttagatcaag tgaccgcaat gggcgtactt ggggataaat
     8941 ccgttcttac cgatttaaaa aaagtcagta aatggcaaaa tgccactaaa acaggggatt
     9001 taagtgaatg tattaccatt gccgaagata gcccgattct accgcagtta gttagcacca
     9061 ccgagagttg cttaggctcg gactgcccgc attttaaaga ttgttatgtg gtacaagccc
     9121 gccgtaaagc gatggaggca gacgtagtgg tggtaaacca tcacctattc tgtgctgata
     9181 tggcagtaaa aaagaccggt tttggtgagt tgatccctga agctgagctg gtgatctttg
     9241 atgaagctca ccaactgccg gatattgcca gccaatattt tgggcaatcg ctttcttccc
     9301 gccagttatt tgatatttgt aaggatacca atattgttta ccgcactgaa ctcaaagatc
     9361 tgtctcaatt aggcaaagcg gctgatcact tgacgaaagt agtgcaagat ttccgattat
     9421 tgatgggcgg agaagggcag attcgaggca atttgcgaga gttatttcgt gataaaaaag
     9481 tggtcgatgg cttggtaaaa gtggctgaaa atattgattt tttaagtgaa gtggcgaaaa
     9541 aatcgctggg gcggtcggaa acgttggata aaattttcga gcggttagca gaagttaagg
     9601 ttcaacttaa aaaattaatg gatactagtg tgacaggcta ttgttactgg tatgaggcga
     9661 atggacgatc ctttgggctg catattacgc cgcttatggt ggcagataaa tttggtgaac
     9721 agttgaagaa ccagcaaatt ggttgggtgt ttacctcagc cacactcgaa gttgggggca
     9781 aattcgatca tttttgtcaa cggctgggca tcgaaaatgc ggagcagttg gtgttacaaa
     9841 gtccgtttga ttaccaacat caatctttac tctgtgtgcc ccgctacttg ccggatacca
     9901 ataaatccaa tactttaact gctctcgggc aattattaaa accggtgatt gaggcgaata
     9961 aagggcgttg tttcttgctt tgtacttcct attatatgat gcgaggttta gccgactttt
    10021 tacgcgaaca cagttcgctg aatgtgttat tgcaaggcga aaccagcaaa agccgtttat
    10081 tggaaaaatt tgtgaaagaa gatcacagcg tgttggtggc gacccaaagt ttttgggagg
    10141 ggattgatgt gcgaggcgat gatttatcgt tggtgattat tgataaattg ccgttcactt
    10201 caccggacga accattactc aaagcccgaa tggaagattg taagatacag ggcggtgatc
    10261 cgtttaacga tattcaaatt cccgaagcgg tgattaccct caaacaaggt gtagggcgtt
    10321 taattcgtga tgtgaccgat agaggtgtag tgattatttg tgattcccgt ttggtgatgc
    10381 gaaattatgg gcaaactttc ttgaaaagtt taccgccgtc tagacgcact agagatttgg
    10441 ggaatgttat tcaatttttg aaacaaaaat agggttacaa gcggtcagat ttctgcagat
    10501 ttttgcaaaa aaacggataa aaataaccgc ttgttgagga gttatatgtt gtctacattg
    10561 ctgcgtatgg ttgcttcttt ggcattgtta tttgtgatgc tttatttagg caagttaatc
    10621 gtttatctat tccccatcgg gatcccggat agtattttag gtatgcttat cttattcggc
    10681 tgcttggtta tcggcattgt taaagtggaa tgggtgatac cgagcgcgaa accgctcaca
    10741 cgttatataa cactattttt ccttccgatt tgtgtcgaac tcatcgaaca tcttgagctg
    10801 ttacgccaaa atctgaacac ttttgtatta gcaacggcat taagcacctt agtttctgtg
    10861 gtcgcgattg gcgtgtttgc acagtggatt tttcattaca agagagggta aaatgagcta
    10921 tctttacact gtgttgacgg taatcatgtt tattgccggt ttgaaaatca gccaaaaact
    10981 caagagcagt tttctgaacc ctttcttaat tgcactcgct ttgctaattg gtgtattact
    11041 gatttttaat atcccttacc aagactatta tcaaggtaat tcccccttga ataacttact
    11101 tggcgtttct gttgtggcac ttgcttaccc tttttatgaa caattacccc atatccgtaa
    11161 aaaatggcga cagattgtcg tgattttgct gtttagtacc atcattacga tgataaccgg
    11221 gcttgggttt tcattattat ttggggcaag ccaagagatc ttagccgcca ttttgcctaa
    11281 atcggtatca atgccaatgg caattattat tacgaatgaa attaatggaa atgttgcaat
    11341 cgcagcggtt ggggtgatga tcgcaggctt agtgggatct attttaggct ttacgcttct
    11401 gcgcctggca aaagtaagaa atgtgatggt gattgggtta agtatgggag ctgcttccca
    11461 cgcattaggc acggcaagat gcttagagca tagtatgaaa gccggtagct atagttctat
    11521 cgcattagtg gcttgtggtg tgctgtcttc tatatttgca cctttaatat ttaacgttat
    11581 cgtcacgtta tgttactaaa aacagtgaaa tctcaaacct tatttggtaa ctggctgaaa
    11641 tcaaggtaca ataccgtgat ttttcaatga aaatagagaa ttatgcaaga acaaataaca
    11701 caagaaaaag atctttctac gcttaaaacc tttaagcgtt tatggccgac tatcagcccg
    11761 tttaaagcgg gtctgattac aggtgctgtc gctttagtat taaatgcgtt agtggattct
    11821 agtcttatct atctcctcaa acccttgcta gatgaaggct ttgggcaagc taataatgat
    11881 tttctgaaat tgatggcggt gcttgtagta gtttttattt tagtgcgcgg tattaccaat
    11941 tatattgcca gttactgttt agcttgggtg tcaggcaagg tggtaatgac attacgccgc
    12001 catatttttc aacatttaat gtatatgccg gtaagctatt ttgataacaa cccgaccggg
    12061 cgtttgctct cacgagtgac ttacgatacc gaaatggtgg cgagttctgc ttctcattct
    12121 ttagtgacta ttgtgcgtga aggggcttat ctgctgtcat tgtttgtggt gatgatctat
    12181 accagttggc agctttctat tgtgttattt gtgatggctc ctatcattgg ggtactgatc
    12241 agtttcgttt caaaatattt tcgtcaatta agtcgcaata tccaaagttc aatgggggag
    12301 cttactgtca cgacagaaca aatgcttaaa gggcataagg ttgtactttc ctttggtggg
    12361 caggaaattg agaaagcacg ttttgatcga gtcagtaatg atatgcgtcg taaagggatg
    12421 aaagtcgttt ctgctgatgg catttctgat ggtgtggtac agcttatcgc ctcttttgcg
    12481 ttatctgctg tgctttatgt tgcgaccttc cccgaggtga tgagtgagaa tttaaccgca
    12541 ggttcattca cagtagtgtt ttcttcgatg atggcaatgt tacgtcctct aaaatcactc
    12601 accaatgtga actcgcaatt ccaacgtgga atggcagcct gccaaacgtt atttgaattt
    12661 ttagatttaa aaactgaaaa agatcacggc ttaatctccg ttgctaaagc tgaaggcaat
    12721 gtggaattta aaaaggtaag ctttagttat gaaggtaaag aggaaaaagc actcgataat
    12781 atttctttca ccattcctaa aggcaaaaca gtagcattgg ttgggcgttc cggctcgggt
    12841 aaatcaacta ttgccaattt gcttacccgt ttttatgatg tgactgacgg tcaaattttg
    12901 cttgatggaa ttaatattca agattaccgc ttaaaaaact taagagaaca atgctcggtg
    12961 gtttctcaac aagttcattt gtttaacgat actattgcta ataatattgc ttatgctgca
    13021 atcgacaaat acagccgaga agaaattatt gctgctgcaa aagctgcaca cgcaatggaa
    13081 tttatcgaaa aattggaaaa tggcttagac actgtaattg gtgaaaatgg cgcaagtctt
    13141 tccggtgggc aacgccaacg cttggcaatc gctcgtgcat tactgcgaaa ttctcctgtg
    13201 cttgtgctgg atgaagctac ctctgcatta gatacggagt cagaaagagc gatccaatct
    13261 gcacttgaag tgctacagaa agatcgtacg gtgttggtca tagcacaccg gttatcaacg
    13321 attgaaaaag ccgatgaaat tttggtagtc gatcacggaa atattattga gcgtggcagc
    13381 catacggaat tgttagcaca aggtggggct tataaacaac tccatagttt acaattcgga
    13441 gcctaatttg taggggagaa atatgtttct ccccaattaa tgataaaaac agggcgaaaa
    13501 atatttcgtc cctacaataa tttatttgga tactatgaaa aaactgacaa aacttttatc
    13561 tcttagtgct ttactggtct taaccgcctg taattcggta ctaaatgcct caacagaggt
    13621 gcaacaacca acagtacaaa ttccgcatga cgatccacaa tggcagcagc atttagcgaa
    13681 actgagccaa attcaaggct atactgcaac ggggcaattt ggctatattt cgcctgaaga
    13741 gcgtttttct tcccatttca actggcgtta tactaatcca atccaatttg ggttagagct
    13801 ttcctcgaat ctctcgacta aatcactgaa attagagcgt aataaacgag gcttaacggt
    13861 ttcggacaat gagggtaata gccgttctga ccgtgatgtt gatgcgttaa tgcaagagat
    13921 tatcggtgtt tcgtttccta tcgatcagtt tgcctattgg gtaaaaggct tgcctgaaaa
    13981 agatggcaac tatattgtga atgaaaaacg ccagctgtcg caatttagtt atccgcttaa
    14041 tggcacgctt tggaaagcaa gctatgtgga gtatcacgaa gatcgtgtac caaacttacc
    14101 gaagctgatt gtgcttgaaa acggcactca aacgttgaag atccgcattg aaaaatgggc
    14161 gtattaatga agaaagtcac cttacccagc ccggcaaaat taaacctgtt tttatacatt
    14221 accaataagc gggcagacgg ttatcacgag ctacaaacgc tgtttcagtt tttagatttt
    14281 ggtgatgaaa ttgagattga gatcacagaa agttcagagg ttgaattaat taatccggta
    14341 gaaggggtgg caactgagga gaatctcatt taccgagcag caaaactttt gcaaaaaacg
    14401 accgcttgta ggcaaggctg ccgtattggg attaaaaaaa acctaccgat gggcggaggt
    14461 gttggtggtg gatcttctaa tgctgcaaca gtattagtcg gtttaaatca cttgtggaat
    14521 accgagcttt cattggaaaa attggcagaa ttaggcttaa gtttaggggc tgatgtgccg
    14581 atatttgtta gaggtatggc tgcttttgcc gaaggcgtgg gggaggtgtt aactccttgt
    14641 gatccacaag aaaaatggta tgtagtgcta aaaccagaag tgtcgatttc aaccgcagcg
    14701 gtgtttaccg acccgaattt acctagaaat acgccaaaac gccccttagc cgaattatta
    14761 tctttaccgt gggaaaacga ttgcgaaaaa gttgtacgag aaaagtattc tgcggttgaa
    14821 gatcttatac aagaattgtt acaatatgct cagtttaggc taacaggcac aggtgcttgt
    14881 attttcgccg aatttgaaac aaaagcagaa gccgagcagg tttttgcaca taaaccggaa
    14941 gccgtcttcg gttttgtggc gaaagggcaa aatatttcgc cattacatca aatgttattc
    15001 caaatctaac catcttaacc ttaaggatca atccatgaca gatattaaac tgtttgcggg
    15061 caacgcaact ccggaacttg ctaatcgtat tgcaaaacat ctttatacct cattaggtaa
    15121 tgcaacagtt ggtcgtttta gcgatggtga aattcaagta caaatcaatg aaaatgtgcg
    15181 tggaggcgat atttttattg tgcaatccac ttgtgcacca actaatgata atttaatgga
    15241 attgatcgtg atggtagatg cgcttcgccg tgcatcagct ggtcgtatta ccgcagtgat
    15301 tccgtatttc ggttatgcac gccaagacag acgcgtgcgt tcggcccgtg tgccaattac
    15361 cgcaaaagtg attgctgatt tcttatccag cgtaggggta gatagagtct taacctgcga
    15421 cttacacgca gagcaaattc aaggtttctt tgatgtgccg gtagataacg tatttggttc
    15481 ccctgtgtta atcgatgata ttttgaaaaa atccgatttg gtcaacccta tcgtggtttc
    15541 tccagatatt ggtggggttg ttcgtgcaag agcggtggca aaattattaa atgataccga
    15601 tatggcaatt attgataaac gtcgtccaaa agcgaatgta tcacaggtaa tgcacattat
    15661 cggtgatgtg gctgatcgtg actgtatttt agttgatgat atgatcgata ctggtggtac
    15721 tttagtcaaa gcggctgaag cgctgaaaga acgtggcgcc cgtcgtgtct ttgcttatgc
    15781 cacacacgcg gtattttcag gcacagcagc tcaaaattta gcgaatcctg ctttagatga
    15841 aattgtagta actgatacta ttccactgag taatgagatt aaagcattaa ataaagtacg
    15901 tgttttaaca ttatccggta tgttagcaga agcaattcgc cgtattagca atgaagaatc
    15961 catttcagca atgtttgatg cctaatttat gagaaaaaac ccagtcgaaa gattgggttt
    16021 ttcatatctg ttcactacta tatttagttt aagataattt gtatgtattc cctgattcgt
    16081 aaattacttt ttgcactaga tacggaaaaa gcacatcaat tctctattca ggcacttgct
    16141 ttagccggaa aatcgccttt ttcgattctg ccaacacccg ataatccgac ccaagtaatg
    16201 ggactcacgt ttaaaaatcc aatagggctt gcagccggag cggataaaaa cggtgaagcg
    16261 atagatggtt ttgggaaatt aggatttggg tttattgagg ttgggactat tactcctctc
    16321 gctcaagacg gaaaccctaa accgaggcaa tttcgtattg tagatgcaga gggaattatt
    16381 aaccgcaatg gatttaataa taaaggcgtt gatgttttag tagaaaatgt aaaaaatgca
    16441 acctatagtg gtattttagg tattaatatt ggaaaaaatg cggttacacc aattgagcat
    16501 agctttgatg actaccaaat ttgtttgcgt aaggtttacc aatatgctga ttatgtggcg
    16561 gtgaatattt catcacccaa taccaaaaat ttgcgcagtt tacaatatgg tgaggcatta
    16621 gatgatttgc tgcgaaacct caaaaatgag caggcacaac tttcgcaaaa aatggggaaa
    16681 tatcgaccgc ttgttttaaa gattgcgcct gatttaaccg atgaagaaat cgtttctgta
    16741 gccgatagtc ttattcgtta ccaaattgat ggggtgattg ccggcaatac taccttgtct
    16801 cgtgaaagta ttgccggatt accgtattcg gaacaactgg gtggattaag tggcaagcca
    16861 ttacactcat taagtacaag gttaatttca aaattatctg ccgagttggg cgagagaata
    16921 ccaattatag gttgtggggg agtccattcg ttagagagtg ggcaggagaa agtgaatgca
    16981 ggtgctagtt tattgcaagt ctattccgca atgatttacc aaggaccatc attaattcaa
    17041 accttagcca aacaccttca gctgaacagt tagggataga tttctagtta aaaatgattg
    17101 gttaggttta tttctgtcat cttagtggaa tagatggcgg tattagctat ctatcaaaaa
    17161 tagacaaaat tgcctatttt tgctatcttg cactgcaaat ttatctacaa aatttaaaca
    17221 aattttataa aattatataa aaatcgttta aaatcaaata gataataagt tt
//
LOCUS       DANLZC010000042        17204 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.042, whole genome shotgun sequence.
ACCESSION   DANLZC010000042 DANLZC010000000
VERSION     DANLZC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17204
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.042"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..214
                     /locus_tag="PXE44_001067"
     CDS             <1..214
                     /locus_tag="PXE44_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5588588.1"
                     /translation="ILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEEREGS
                     RIAVVLFQQVRVFHRPHPNPDTDKGR"
     gene            257..586
                     /locus_tag="PXE44_001068"
     CDS             257..586
                     /locus_tag="PXE44_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5588589.1"
                     /translation="MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQL
                     KKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSPELHKAAVIAATAENLSLNEWIN
                     QTLEKSV"
     gene            complement(625..1011)
                     /gene="cueR"
                     /locus_tag="PXE44_001069"
     CDS             complement(625..1011)
                     /gene="cueR"
                     /locus_tag="PXE44_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDL5588590.1"
                     /translation="MNISQAAEKTGLSAKQIRDYEKAGLLPPTSRTEAGYRFYNATDL
                     SRLHFISNARKVGFSLAQISELLKLNDNPNRSSRDVKQLTEQHIGELEQKIADLNQML
                     TLLKSWSSSCCGNANSECSILNGLNG"
     gene            1100..1300
                     /locus_tag="PXE44_001070"
     CDS             1100..1300
                     /locus_tag="PXE44_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL5588591.1"
                     /translation="MTITLQLDGLHCGNCVKSVEKALNGVAGVNQVSVTLEPQIAVVE
                     GSAEAQALIEAVEDIGFDAKLA"
     gene            1353..3545
                     /locus_tag="PXE44_001071"
     CDS             1353..3545
                     /locus_tag="PXE44_001071"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL5588592.1"
                     /translation="MTSTAHYSPEQQLLIDGMHCAACVRRVEKALLKVEKVEFASVNL
                     ADQTAFVQGNANPEALVAAVVKIGFGAEVLESEEERRIKQQAQTQRVLGHKRTQFMLA
                     LVVGFALVAFGFYKGMTVNETNRSLWLSWAGISLATMYFSGKDFFLGAFSALKNRSAN
                     MDMLVAISTGTAWLYSCWLTLFPQPNAHVYFEASVMILGFINLGKYLELKAKQRSSLA
                     LEKLLDLAPKQAVIFEENIAKTIPAKAIKPQMRVQALTGDRLAVDGILDSGSIWVDES
                     MLTGEALPVEKKIGDKVRAGTLVQDGSGTYIAEQVGSQTALARVINAVRHAQSSKPPL
                     AQFVDKIAAVFVPVVVSIALLSALIWLAVGQDFAFALSIFTTVLIIACPCALGLAIPL
                     STIAGVARSAEFGVLVRNIEALQASSEIDTLVFDKTGTLTTGRMEVSDVLTFNGFEQK
                     RLLQLAKSVEQHASHPIAKAIAKFAENQTACGVTDVQVLKGLGISAKYEGSTIRMGNW
                     QFVGETIQANLASLLPAKEGDFSSKIFVSVDNQLAGVLSVSDQLRPEAKATIHQFQAE
                     GYQCVMLTGDRQQTADYFANALGLDGVIAEVLPEQKAEKIRALQAEGKKVAMIGDGIN
                     DAPALAQANVGIAMHNGSDIAVETADLSLMREGLAPLMQILPFSKKVLKNMKQSLLGA
                     FIYNVIAIPIAAGVLYPFTGWLLNPMIAAIAMTLSSITVVLNSQRLLK"
     gene            complement(3598..4383)
                     /gene="truA"
                     /locus_tag="PXE44_001072"
     CDS             complement(3598..4383)
                     /gene="truA"
                     /locus_tag="PXE44_001072"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL5588593.1"
                     /translation="MKIALGIEYDGSRYFGWQRQEAVESVQQQLEQALSVVANSPVEV
                     FCAGRTDAGVHGTGQVVHFETQANRPLQSWCFGTNAHLPDDIAVKWAVAVNEDFHARF
                     SATARRYRYIIFNQKLRSAILPKGVTHFHFPLDERKMHQAGQYLLGENDFSSFRAAKC
                     QSNTPWRNIHHLNVSRQGNYVIVDIQANAFVHHMVRNIVGTLLEIGQGRQPVEWAKWV
                     LEQRDREKAAPTAKAEGLYLVEVHYPEHFGIPKTPLGPLFLAD"
     gene            complement(4448..5137)
                     /gene="folE"
                     /locus_tag="PXE44_001073"
     CDS             complement(4448..5137)
                     /gene="folE"
                     /locus_tag="PXE44_001073"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDL5588594.1"
                     /translation="MTTISPELTITENTLSPEAQKVRQALLAKGIETPTVTHTKDKAS
                     RRAEIQQHMQAVLELLGLDLSDDSLEETPHRLAKMYVDEIFSGLDYATFPKITNIENR
                     MKVSEMVLVDDITLTSTCEHHFVTIDGKVAVAYYPKKWVIGLSKINRVVQFFAQRPQV
                     QERFTEQILTAFQTILETDDVAVYVKATHFCVKCRGIKDTNSYTVTSAFGGVFLEDRE
                     TRKEFLGLLNK"
     gene            5238..5945
                     /locus_tag="PXE44_001074"
     CDS             5238..5945
                     /locus_tag="PXE44_001074"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HDL5588595.1"
                     /translation="MQNIQYLFNADLIPEHQAEFVQSLLTCKNFDNSDRLLGFSKGRG
                     TTWFLDTSAELGINVVLRHYYRGGLFGKIIKDSYFFTRFEATRAYKEFFLLQQMLAWG
                     LPVPRPVAIKIERHFCCYRADIMLEKLENTQDLSKILQSHSLSNEQYEQIGKLIRSLH
                     NHQIHHSDLNIHNILLGNDGKFCLIDFDKCGIEPGENWKQNNLDRLLRSFKKEQGRLN
                     IFFSEENWQAVLRGYTL"
     gene            complement(6000..7097)
                     /gene="apbC"
                     /locus_tag="PXE44_001075"
     CDS             complement(6000..7097)
                     /gene="apbC"
                     /locus_tag="PXE44_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDL5588596.1"
                     /translation="MNQLNEQQLVEIKAVLQQFSHPTLQKDLIALNAFKKAELGGDIL
                     RIEITMPFAWNSGFETLKAETEAKLKQVTGSSGVKWVLNYQIATLKRANKHPAVNGVK
                     NIIAVTSGKGGVGKSTTSVNLALALRAQGTRVGILDADIYGPSIPHMLGAKDQRPTSP
                     DNKHITPITAHGLQSNSIGYLMEEDNATIWRGPMASSALSQLLNETWWNELDYLVIDM
                     PPGTGDIQLTLSQQIPVTGAVVVTTPQDIALLDAIKGISMFEKVSVPVLGVIENMSMH
                     ICSNCGHHEAIFGTGGAEKIAQKYGTKVLAQLPLHIRLREDLDSGVPTVVAAPEHEIS
                     QAYLALAEKVASELYWQGSVIPSEIMIREVK"
     gene            complement(7094..8641)
                     /locus_tag="PXE44_001076"
     CDS             complement(7094..8641)
                     /locus_tag="PXE44_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5588597.1"
                     /translation="MLKSLQAVTFVKKIAKVTACILLITSCDQLICPQSIQSVWVENR
                     QSDTLLKRAIYSAVFQLDPHKIKASADAAPLRDLLVGLTAYNRKGDVVPAIAQNWFTE
                     DEKEWLFILDEKAVWSNGQPVTAQDFVASWRRLIDPQNHSVLSKYLIYMGIENAKAIE
                     EQIKSVEELGVEALNAHTLRIRLNHPNHQLPEMLAHSALLPTYQGIAPTIDNFVSNAS
                     YRLEKLDAQKMVLKARTQEIPFQTIEYDLITTIQNCSAFDIVENPLLNKKTNIVKFPR
                     LCSYYYEFNFDDPLLAKREIREAIRTMVASANSGQEYGVPSQSVIPHSLVKDREKRWN
                     PVVVEQLLTKAGISPYNPLKLTLLYDENSINIANQVTRALSQSELFNITPKMVDWQTL
                     NKVREQKDYQIARAGWCADYPDPIVFLQKFHSMSPDNHSGYKSEIVDQKLNRLQTETL
                     SAEARESLIQEISEQLYSDVAVLPLFQYHHRVGIVSSLLGIDLNNDSEVIYSKDLKRN
                     LANKDNQ"
     gene            complement(8667..9317)
                     /locus_tag="PXE44_001077"
     CDS             complement(8667..9317)
                     /locus_tag="PXE44_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HDL5588598.1"
                     /translation="MDIIINFAIYLQFFIGLFAIVNPFGSLPIFFSMTAHQYEGERNR
                     TSLITAVSIGVILLVSLYFGNLILNAFSISLSSFRVAGGILIVSIAMTMISGKLGEHK
                     QNKEEKNEDITEYENIGVVPLAMPIMAGPGAIGSTIVWGTRYHTWEDYVGFSIAIIVF
                     ALICYILFRYSAPLVKKLGKTGSNVVTRIMGLILMALGIEIIVAGITTLFPVLLVK"
     gene            complement(9439..11310)
                     /locus_tag="PXE44_001078"
     CDS             complement(9439..11310)
                     /locus_tag="PXE44_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HDL5588599.1"
                     /translation="MIEKMHERTNGPVFKIIFALVSISFVIGGIGTGLISQDNSVAKV
                     NGEEISQQLFNNTLNREQNRLNAELGSRFWDLMDSPEYAAQFNQSVLNGLIDEELLRQ
                     YAKELKLGISADQIKSEIVNSQMFQQDGKFSNELYQQTLRHNNLSADGYAAIVQEGML
                     QSQLQEGIINSDFNVPAQQALLAKLLLQQREVRLAEFSVAKEMENQTASEQELRAYYE
                     ANKTKLLAPEKLAVEYVTLSPKDVESKVEITNEQIQTYYDRNKADYVTKGEAHLAHIQ
                     VASEAEAQAIEQALKNGEDFATLAKNKSSDTLSANNGGDLGWAKAGTFPKAFEDAVAN
                     LTAGQMSAAVKVDNAYHIIKVLERKPEQVIELAQVKDKIAQTIRQELVLTEYFTVARE
                     MANKAFENNSSLEEVAKAGNVELQKTDLFTRENVPTALQNEKVLKVLFEGELRQNKQN
                     SDALDVGNDANPQTLFVRVSDYDAERPQTFDEAKAAVENFVKAEKAEKALLAKVEENV
                     KALNEGKSVDVKFNAPQTLVYMQAEVENPVLAGTIFAMKKAQDKTTYQMARNQKGDVV
                     IVALDNITEGNAEQFKTLAPQFEQANRLILRNEFLQSLRAKASIEVNEDFMQQRSSH"
     gene            11492..11761
                     /gene="rpsO"
                     /locus_tag="PXE44_001079"
     CDS             11492..11761
                     /gene="rpsO"
                     /locus_tag="PXE44_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL5588600.1"
                     /translation="MSLSVEAKAKIVAEFGRDAKDTGSSEVQIALLTAQINHLQAHFA
                     EHKKDHHGRRGLLRMVSRRRKLLDYLKRTDLAKYSETIARLGLRR"
     gene            11833..12702
                     /locus_tag="PXE44_001080"
     CDS             11833..12702
                     /locus_tag="PXE44_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDL5588601.1"
                     /translation="MAFASLFTLLDDIAAVLDDVSIMTKVAAKKTAGVIGDDLALNAN
                     QVSGKNVVPERELPIVWAVAKGSLINKVILIPLALLLSVFLPQAIIPLLMIGGAYLCF
                     EGVEKLLHRLLHKNNQAHSDETETLSEKDKIKGAIRTDFILSAEIIIIALGVLQDSST
                     LTQILSLSAIGVGITLFVYGLVGLIVKLDDIGLWLMQKKSTFSQAVGKGLLFAMPWFM
                     KGLSVIGTIAMFLVGGGIFTHNLPMIHHFVEQFGISPHFSTLVDFAVGIVVGIIACLI
                     ILPLMKLFSKKAH"
     gene            complement(12792..14057)
                     /locus_tag="PXE44_001081"
     CDS             complement(12792..14057)
                     /locus_tag="PXE44_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HDL5588602.1"
                     /translation="MFRTLFLMLLLLVGLISGPYLAGNQGYVRIETATTVIEMSIVML
                     VVFFVLAMAVVYLLEWIVTKICRMSNGAYNWFSTRKHKKAQQETLEGLMRMSEGNYSK
                     AEKLFSKNAKHADEPVLNLIKAAEAAQQNGDDLAANKYLIKATEIAGPNNVAVELVRT
                     RILLQQGKLPAARTAIDSLLELAPHSDEVLRLAIQIYKESRAYVALDRLLVEIGQRNF
                     LTAEEYAELEYFVDDGLQDERLQEEGQEGLLSWWENQPNRRRKSVYSRVGVIKRLIDS
                     DDHQSAQEIALETLKKFEDEQLQGLFVQLTRLQIDSDSKLLKVLAKRADKANVKYTDD
                     YARALGYIYTRDGQYQKAKSQFELLLAHDECVADDRIMALHVAEQMGDTLMANRIREE
                     NLKQVNMATQSKVNEPLALPQTATESNSL"
     gene            complement(14076..15386)
                     /locus_tag="PXE44_001082"
     CDS             complement(14076..15386)
                     /locus_tag="PXE44_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDL5588603.1"
                     /translation="MSRSKKVTEHLEHSAEKVVEEAQQAVDSAGSFAKKEIEEDVVNE
                     AIEKDIQTEMQKAPKEQVVVQKSGGKGLALVALLVALAVGGAGHYMANKKFAEVESQI
                     QAVSAKANQPTTQTMVEMPNFENEKAQIAELVTEYQKALERIKALETAQNGYTQQISS
                     LQLQLQKLNNTSGSDNTFWLLSEADFLLNNAARKVVLDNDIETAKNLLLEADQVLSKA
                     PNTIHIREAIKTDLNTLTALHQIDQNALMQRVANLANKLDDLPILESEQASNSKQDGQ
                     VSDSLADWEKNLEKSAASFLDHFIRVSKRNAADEKAFVAPNQEIYLRENIRLRLQIAI
                     LAVPRQQSELYKKSLQTVGSWVRSYFETSNENVQHFLKELDELAEQTIYVDAPTSLQS
                     LKVIAQQLNKTPEKIEKVEIKAEKELEQAEPVKAEQDAMPLSAQ"
     gene            complement(15398..16162)
                     /locus_tag="PXE44_001083"
     CDS             complement(15398..16162)
                     /locus_tag="PXE44_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDL5588604.1"
                     /translation="MNVLVTRLDSRGQELVDMLNEQQIFAIHQPLFTIEAGKELPLLP
                     SAISRLKSDDYVFVVSPNAVDYAAKTLVDTGFHFRSDLKYFAVGQRTAKYLTEKAEQA
                     VIYPIEFENSEGVLALPEMQNLTDKTILILRADSGRELLAETAVLRGATIQYLECYRR
                     EPVTDDIPEKISLCKRLGVDTIVITSSEILRSLYEQAKADCRAWLFECDLVVVSRRIA
                     KIAKQMGWQEDKIRVSDKADNVTLMNTLLFKVGKSN"
     gene            complement(16162..17097)
                     /gene="hemC"
                     /locus_tag="PXE44_001084"
     CDS             complement(16162..17097)
                     /gene="hemC"
                     /locus_tag="PXE44_001084"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDL5588605.1"
                     /translation="MKDILRIATRQSPLALWQANFVKSELEKHFPQLSVELVTMVTKG
                     DIILDTPLAKIGGKGLFVKELELALLENRADIAVHSMKDVPMSFPEGLGLAVICERED
                     PRDAFVSNNYHSLEDLPKGAVVGTSSLRRQCQLMAKYPHLEVKSLRGNVGTRLSKLDN
                     GEYDAIILASAGLIRLGLQERIRSYISVEQSLPAAGQGAVGIETRANDERILNYIAKL
                     NHSSTACCVMAERAMNTRLQGGCQVPIGGYATLEGDELTLNALVGSLDGSQIIRASAK
                     GHKQEAEQLGILVAEQLLAQGADKILAAVYNENVQ"
BASE COUNT         4897 a         3717 c         3241 g         5349 t
ORIGIN      
        1 aatcctaaag cggaaatctc aaaagacact acaacaaatc tacacccagc ctatttctgc
       61 caatattaaa tggacaaata tagaagctct ttttctagag ttaggagcag aaatagaaga
      121 acgagaaggg agcagaattg cagttgtttt atttcaacag gtcagagtat ttcatcgccc
      181 acacccgaat cctgatacag ataaggggcg gtagcgagta ttaaaaaatg gttatctgaa
      241 aacggagtca ttccttatgc gtaatattat ggatattaat ggttataaag ctgtcattgc
      301 ttacgatccc gaaacagaat tatttcgagg tgaatttatc ggattaaatg gtggagccga
      361 tttttatgcc gataatgtca ttcaattaaa aaaagaaggt gaactaagtt tgaatatctt
      421 tttagaactt tgtagagaaa aaggtattga gccgttcaaa caatattccg gaaagtttaa
      481 tgttcgcctt tcgcctgaat tacataaagc tgcggtaatt gcagcaactg ctgaaaattt
      541 aagcttaaat gaatggatta accaaacgtt agaaaagagc gtgtagccta attgcaaaac
      601 aatcacctat tttgaccgct tgtattaccc gtttaaccca ttcaatatcg aacactccga
      661 attggcgttg ccacagcaag agctactcca gcttttgagc aaagtcagca tttggtttaa
      721 atcggcaatt ttttgttcaa gctcaccgat atgctgttcg gttaattgtt ttacatctcg
      781 gcttgagcgg tttgggttgt cgtttaattt aagtagttcg ctgatttgag ccagcgaaaa
      841 gcccactttt cgggcattgc tgataaaatg taatcggctc aaatcagtag cattgtagaa
      901 tcgatacccc gcttcggtgc gacttgtcgg cggcagaagt cctgcttttt cataatctcg
      961 gatttgtttg gcggaaagcc cggttttttc tgcggcttgg ctaatattca ttataatcct
     1021 ctcactttaa tcgcttgacc ttaacctaag gttaagcttt attatacgcc tgaataatca
     1081 ctcaaaacag gagaaaatga tgacaattac cctacaactt gatggcttac attgtggcaa
     1141 ctgtgtgaaa agtgtcgaaa aagcgttaaa tggcgtggct ggggtgaacc aagttagcgt
     1201 aacgttagaa ccgcaaattg ctgtggtgga aggctcagca gaagcacaag ccttaattga
     1261 agcggttgaa gacattggct ttgacgcaaa attagcctaa ttaataaggg gcagaaattt
     1321 tgccctaatc tgcccaatat ttcggaggat aaatgacaag cacagctcac tattcgccag
     1381 aacaacaact tttgattgat ggaatgcact gtgccgcctg tgttcggcga gtggaaaaag
     1441 ccttacttaa agtggaaaag gtggaatttg cctcagtcaa tcttgccgat caaaccgctt
     1501 ttgtgcaagg caacgccaac cctgaagcct tggtagcagc ggtagttaaa atcggttttg
     1561 gggcagaagt gctggaaagc gaggaagaac gccgaattaa acaacaagcc caaacgcaac
     1621 gagtgttagg gcataaacgc actcaattta tgcttgccct tgtagttggt tttgccttag
     1681 ttgcttttgg gttctacaaa ggaatgacgg taaacgaaac caatcgttcg ctttggctca
     1741 gctgggcagg tattagcctt gcgacaatgt atttttcagg taaagatttc tttcttgggg
     1801 cattttcagc actgaaaaac cgctcagcca atatggacat gttagtagca atcagcaccg
     1861 gcacggcttg gctctattct tgttggctca cgcttttccc acaaccgaat gctcacgttt
     1921 attttgaagc cagcgtgatg attctcggct ttatcaatct aggaaaatac ttagaactga
     1981 aagccaaaca gcgttcctcg ctagcattgg aaaaattgct ggatctcgcc ccaaaacaag
     2041 cggtcatttt tgaagaaaat attgcaaaaa ccattccggc taaagcaatt aagccacaaa
     2101 tgcgggtgca agccttaacc ggtgaccgct tggcggttga tggtatttta gacagtggct
     2161 ctatttgggt tgatgaatca atgctcaccg gtgaagccct acctgtagag aaaaaaatcg
     2221 gtgataaagt gcgagccggc actctggtgc aagacggttc aggcacttac attgccgaac
     2281 aggtcggctc acaaaccgct ttggctcgtg taatcaatgc cgttcgccac gcccaaagca
     2341 gcaaaccgcc attggcacag tttgtagata aaatcgccgc tgtttttgtg ccggtggtgg
     2401 tttcaatcgc attactttct gcgttaattt ggctggcagt tgggcaggat tttgcctttg
     2461 cactctcgat ttttactacc gtgttgatca tcgcttgccc ttgtgcattg gggctggcaa
     2521 ttccgctttc taccattgcc ggcgtggcac gttctgccga atttggcgtg ctggtgcgaa
     2581 atattgaggc actacaagca agcagtgaaa tcgacaccct agtgttcgat aaaaccggca
     2641 cactcaccac tggcagaatg gaagtgtcgg atgttctcac ctttaacgga tttgagcaaa
     2701 agcgcttgct acaacttgct aaaagtgtag agcaacacgc ctcacacccg attgcgaaag
     2761 cgattgcaaa atttgccgaa aatcagaccg cttgtggtgt aaccgatgtg caagtgttga
     2821 aagggttagg catttcagca aaatatgaag gcagtacaat cagaatggga aattggcaat
     2881 ttgtaggcga aaccattcaa gcaaatcttg cctccctatt gcccgctaaa gagggggatt
     2941 tttcctcaaa aatttttgtt tcggtggata atcaactcgc cggtgtattg tcggtttcag
     3001 accaacttcg ccctgaagcg aaagcaacca ttcatcaatt ccaagcggaa ggctatcaat
     3061 gcgtgatgct gaccggtgat cgccaacaaa ctgccgacta tttcgccaac gcattggggt
     3121 tagacggcgt gattgcggag gtattaccgg agcaaaaagc ggaaaaaatt cgagccttgc
     3181 aagcagaggg taaaaaagta gcaatgattg gcgacggcat aaacgatgct ccagctctgg
     3241 ctcaagccaa tgtgggcatt gcgatgcaca atggttcaga tattgcagtg gaaaccgccg
     3301 atctttcgct gatgcgagaa gggctagcac cactgatgca aattctaccg ttctctaaaa
     3361 aagtgctgaa aaatatgaag caaagtttgc tcggggcttt tatctataat gttattgcga
     3421 ttcctattgc tgccggcgtg ctttaccctt tcaccggctg gctactcaac ccaatgattg
     3481 ccgccattgc gatgacattg tcgtcaatta ccgtggtgtt aaatagtcaa aggttgttga
     3541 aataatttaa gcggtcaaat ttgcatattt ttttgcaaat ttgaccgctt gtaatcacta
     3601 atccgccaaa aacagcggcc caagaggtgt tttcggaatg ccaaaatgct cagggtaatg
     3661 cacctcgacc aaatatagcc cctcggcttt cgctgtaggg gcagcttttt cccggtcacg
     3721 ttgctccaac acccattttg cccattccac aggctgtctg ccttgcccga tttcgagcaa
     3781 tgttcccaca atattgcgaa ccatatgatg tacaaaggcg ttggcttgaa tatcaacaat
     3841 cacataattg ccttgccgag acacatttaa atgatgaata ttccgccacg gcgtattaga
     3901 ttggcatttg gcggcacgga aagaggaaaa gtcattctcg cccagcaaat actgccctgc
     3961 ttggtgcatt ttgcgttcat caaggggaaa gtgaaagtgg gtaacacctt taggtaaaat
     4021 tgccgaccgc agtttttggt taaaaataat atagcggtaa cggcgggcgg tggcactaaa
     4081 acgggcgtga aaatcctcat tcaccgctac cgcccatttc accgcaatat catcgggtaa
     4141 atgagcgtta gtgccgaaac accagctttg tagcgggcgg tttgcttgcg tttcaaaatg
     4201 cacgacctgc cctgtgccgt gcacaccggc atcggttctg ccggcacaaa acacttctac
     4261 gggcgaattg gcaacaaccg acaacgcctg ctctagctgt tgttgtacac tctctaccgc
     4321 ttcttgtcgt tgccagccaa aataacggct gccgtcatat tcaataccta atgcgatttt
     4381 cattttaata ccttgataaa aagaggggcg aaaaattttt cgcccccaca gtaattgata
     4441 tttgcaatta tttattcaat aagcctaaaa attctttgcg ggtttctcgg tcttctaaga
     4501 aaacaccgcc aaaggcagaa gtaactgtat agctattggt gtcttttatg ccacggcatt
     4561 tcacgcaaaa atgggtggct ttcacataca ccgcaacatc gtctgtttct aaaatagttt
     4621 gaaaggcagt taaaatctgt tcggtaaaac gctcttgcac ctgcggacgt tgggcaaaaa
     4681 actgcacgac acggttgatt ttcgagagtc caatcaccca ttttttcggg taataggcta
     4741 ccgctacttt gccgtcaata gtaacaaaat gatgctcaca ggtagaagtt aaagtaatat
     4801 cgtccaccag caccatttca ctgactttca ttcggttttc aatattggta attttcggga
     4861 aggtagcata atcaagcccg ctgaaaattt catcaacata cattttcgcc aaacgatgag
     4921 gcgtttcttc taagctgtca tcacttaaat ctaaaccgag caattctaaa actgcctgca
     4981 tatgctgctg gatttctgct cggcgactgg ctttatcttt ggtatgagta acagtcggag
     5041 tttcaatgcc ttttgccaac aaggcttggc gaactttttg agcctccggc gaaagcgtgt
     5101 tttctgtgat agttaattcg ggcgagatcg ttgtcattgg ttttcctctc aaatgtaagc
     5161 tgaagattct aacaaattga catcaaaaaa aatagctcta aaatgagcta tttcagccat
     5221 aaacaccctc tttatttatg caaaatatac agtatctctt taatgccgac ctgattcccg
     5281 aacatcaagc tgaatttgtg cagtctctct taacttgcaa aaattttgac aattccgacc
     5341 gcttgctagg cttctcaaaa gggcgaggta cgacttggtt tttagatacc tctgctgaac
     5401 taggtatcaa tgtggtgctt cgccattatt atcgtggcgg cttgttcggt aaaatcatca
     5461 aagatagtta cttttttact cgctttgagg ccacccgagc gtataaagaa tttttcttac
     5521 tgcaacaaat gttagcttgg ggtttacctg tgcctcgccc tgtggcgatt aaaatagaaa
     5581 gacatttttg ttgctatcgt gccgacatta tgctcgaaaa gctagaaaac acacaagatt
     5641 taagcaaaat cttgcaatcc cattctttat cgaacgaaca atatgagcag atagggaaac
     5701 tgattcgcag tctgcataac catcaaattc accattccga cttaaatatc cataatattt
     5761 tgcttggtaa tgatggcaaa ttttgtctga ttgattttga taaatgcggc attgagccgg
     5821 gcgaaaattg gaagcaaaat aacctagacc gcctgttacg ctcgtttaag aaagaacaag
     5881 ggcgattaaa tattttcttc agcgaagaaa attggcaggc tgttttgaga ggatatactc
     5941 tctaagaaac aataatggca caagcttaga aacttgtgcc atcaaatcat caatattaac
     6001 tacttcactt cacgaatcat aatttcagac ggaatcacac tgccttgcca gtaaagttca
     6061 gaagctactt tttccgctaa tgcgaggtag gcttggctga tttcgtgttc cggagctgcc
     6121 accaccgttg gcacaccgct atctaaatct tcacgcagac gaatatgtag tggtaattgt
     6181 gctaacactt tagtgccata tttttgggcg attttttccg ctccgccggt gccgaaaatt
     6241 gcctcatggt gtccgcagtt tgagcagata tgcattgaca tattttcaat caccccaagc
     6301 actggtactg agactttctc aaacattgag atccctttaa tcgcatctaa taaggcaata
     6361 tcttgtgggg ttgttactac caccgcaccg gtcaccggaa tttgctgtga aagcgtaagc
     6421 tgaatatcac ccgtacccgg tggcatatca atgaccaagt aatccaattc attccaccaa
     6481 gtttcattta aaagctgact taacgcactg cttgccatcg ggccacgcca aatggtggcg
     6541 ttatcttctt ccattaaata gccgatagaa ttagattgca agccgtgagc ggtaatcggc
     6601 gtgatgtgct tgttatcagg agaagttgga cgttggtctt ttgctcccaa catatgcgga
     6661 atagatgggc cgtaaatatc cgcatctaaa atccccactc ttgtcccttg tgctcgtaag
     6721 gctaaagcaa gattaactga ggtcgttgat ttccctaccc caccttttcc tgaagtgacc
     6781 gcaatgatat tttttacccc attgactgcc gggtgtttat tcgcccgttt taaggtcgca
     6841 atttggtaat ttaataccca ttttacaccg cttgaacctg ttacttgctt taatttagcc
     6901 tctgtttcgg cttttaaggt ttcaaaacca ctgttccacg caaatggcat cgtgatttca
     6961 attctaagaa tatcgccccc taattctgct tttttaaacg cattaagggc aattaaatct
     7021 ttctgtaaag tcgggtggga aaattgttgc aagactgctt taatttcaac aagttgctgt
     7081 tcatttaatt ggttcattgg ttatccttgt ttgctaaatt gcgttttaaa tctttgctat
     7141 aaattacttc gctatcgtta ttcaaatcaa tacccaaaag ggaggaaaca atcccaaccc
     7201 gatgatggta ttgaaataat ggcaatacgg caacatcgct ataaagctgc tcgctaattt
     7261 cttgaataag tgactctctc gcttctgcac taagcgtttc tgtttgtaaa cgatttaatt
     7321 tttgatcgac tatctcactt ttatagccgg aatgattatc cgggctcatt gaatgaaact
     7381 tctgcaaaaa tacaatcgga tcaggataat ctgcacacca gcctgctcgt gctatctggt
     7441 aatctttttg ttcccttact ttatttaaag tctgccaatc aaccatttta ggtgtaatat
     7501 taaataattc tgattgagaa agggctcgag taacttggtt agcaatatta atgctattct
     7561 catcatataa caaagtcagc tttaaaggat tataagggct aattcctgct tttgttaata
     7621 gctgctcaac cacaacagga ttccaacgct tttctctatc ttttactaag ctatgtggaa
     7681 ttacagactg actcggaacc ccatattctt gcccactatt tgctgaagcc accattgtcc
     7741 ttattgcttc acgaatctct cgtttagcta ataatggatc atcaaaattg aactcatagt
     7801 aataagagca caaacgcgga aatttcacga tattcgtttt tttatttaat aaaggattct
     7861 cgacaatatc aaaagcagag cagttttgga tagttgtaat taaatcatac tctatagttt
     7921 gaaagggaat ttcttgggtt cgagccttta acaccatttt ttgagcatct agtttctcga
     7981 gacggtaaga cgcattgctc acgaagttat ctatggttgg agcaatccct tgataagtag
     8041 gtaataatgc ggaatgtgcc agcatttcag gcaattggtg atttgggtga tttagccgaa
     8101 ttctcaatgt atgcgcattt aatgcctcta caccgagttc ttcaactgac tttatttgct
     8161 cttcaatcgc ttttgcattt tctataccca tatagatgag atatttagac aatacagaat
     8221 gattttgagg atctattaaa cgtcgccagc ttgctacaaa atcttgtgca gtcacaggtt
     8281 ggccattaga ccacacagct ttctcatcta aaataaacaa ccactctttt tcatcttcag
     8341 taaaccaatt ctgagcaatg gccggaacga catcaccttt tctgttgtag gccgttaaac
     8401 cgactaatag atctcttaaa ggagcagcat ctgcagaagc tttaatttta tgtgggtcga
     8461 gttgaaatac agcagaataa attgcacgtt tgagtaaagt atcactctgc cggttttcta
     8521 cccagacaga ttgaatggat tgagggcaaa ttaattgatc acaagatgta ataagcagaa
     8581 tgcaagcggt aacttttgca atttttttta caaatgtaac cgcttgtaag gatttcaaca
     8641 taaactcccc aaaggttaat taagatttat tttacaagaa gcacaggaaa taaagttgta
     8701 attcctgcca caataatttc aattcccagc gccattaaaa tcagccccat gatacgagta
     8761 acgacattag atcctgtttt gcctaatttt ttcaccaaag gggctgaata gcggaataaa
     8821 atataacaaa ttaatgcaaa tacaataatc gcaatgctaa agccaacata gtcttcccaa
     8881 gtatggtagc gggttcccca cacaatggtt gagccaattg cccctggtcc tgccataatc
     8941 ggcattgcta atggcacgac accgatattt tcgtattcag taatatcttc atttttctct
     9001 tctttatttt gtttgtgttc acctaattta ccgctaatca tcgtcatcgc aatgcttaca
     9061 atcaaaatgc caccagctac tctaaaagag ctaagtgaaa tgctaaacgc attgagaatc
     9121 agattaccaa aatagaggct gactaataaa attactccaa tagaaacagc ggtgattaag
     9181 ctggtgcgat ttctttcccc ctcatattgg tgagcggtca tactgaaaaa aataggcaag
     9241 gaaccaaaag gattgactat agcaaataga ccaatgaaga attggaggta gatggcaaaa
     9301 ttaataatga tatccacaaa aactttctca aacaacagtg tttcgggcgc tattatatcg
     9361 gttaatcaag gtagatacaa accaaaagca cccagcctaa aggacggttg ggtgcttttt
     9421 gataaaatga aaggcttgtt agtgtgacga acgttgctgc ataaaatctt catttacctc
     9481 aattgaagct tttgcacgta gcgattgtaa gaattcatta cgtaaaatta aacgattagc
     9541 ttgttcaaat tgtggcgcca atgtcttaaa ctgttcagca ttcccttccg taatgttatc
     9601 taaggcaaca ataactacat cgcccttttg attacgtgcc atttgataag tcgttttatc
     9661 ttgcgccttt ttcattgcga aaatcgttcc agccaatacc gggttttcta cttctgcttg
     9721 catataaact aaagtttgtg gcgcattaaa tttcacgtcc actgatttcc cttcattcaa
     9781 ggcttttaca ttttcttcta ctttcgccag caacgctttt tccgcttttt ctgccttcac
     9841 aaaattttca actgctgctt ttgcttcatc aaaagtttgt ggacgttctg catcataatc
     9901 actcactctc acaaacaatg tctgtggatt tgcatcattc ccaacatcta aagcatcaga
     9961 attttgttta ttttggcgta gctctccttc aaacaaaact tttaatactt tctcattttg
    10021 taaagcagta ggtacgtttt cacgggtaaa cagatccgtt ttttgcagtt ccacattacc
    10081 ggctttcgca acttcttcaa gtgaagaatt attctcaaag gctttatttg ccatttcgcg
    10141 cgcaactgta aaatactcag ttaacactaa ttcttggcga atagtttggg caattttatc
    10201 tttcacttgt gctaactcaa tcacttgctc aggtttacgc tccaatactt tgatgatatg
    10261 gtaagcatta tccactttca ccgctgcact catctggccg gcagttaaat ttgctacagc
    10321 atcttcaaaa gctttcggga aagtgccagc ttttgcccaa cctagatcac caccattatt
    10381 tgctgataaa gtatcgctag atttgttttt tgccaaagta gcaaaatctt ccccattttt
    10441 gagcgcttgc tcaatagcct gtgcttccgc ttcactagcg acttgaatat gagctaaatg
    10501 ggcttcacct ttagttacat aatcggcttt attgcgatca tagtaagttt gaatttgctc
    10561 attggtaatc tcaactttgc tttccacatc ttttggagaa agagtaacgt actcaaccgc
    10621 taatttttca ggtgctaata atttagtttt atttgcttcg taataggctc gcaattcttg
    10681 ctcggaagcg gtctgatttt ccatttcttt tgcaactgaa aattcggcta aacgcacttc
    10741 acgttgttgc aacagtaatt ttgctaacaa tgcctgctgt gccggtacat taaaatcact
    10801 attgataatc ccctcttgta gctgggattg caacatacct tcctgcacaa tcgctgcata
    10861 gccatcggca cttaaattat tgtggcgtaa tgtttgttgg tataactcgt tactgaattt
    10921 accatcttgt tgaaacatct ggctgtttac aatttcagat ttgatttgat cagcactaat
    10981 acctagtttt aactcttttg catactggcg cagaagctct tcatcaatta atccatttaa
    11041 taccgattga ttaaattgag cagcatactc cgggctatcc attaaatccc aaaagcgact
    11101 ccccagttca gcatttaatc tattttgctc acgattaagc gtgttattaa ataattgctg
    11161 actaatttct tccccattta ctttcgctac agaattatct tgtgaaatca atcctgtccc
    11221 aattccaccg attacaaatg aaatagagac taatgcaaaa ataattttaa agaccggccc
    11281 atttgtccgc tcgtgcattt tttcaatcat ttaaaaattc ctaaattact caaaaattgt
    11341 cgctgattat actctttctc aaatgagaaa taaacttgca aatttttatt gaaatttaac
    11401 cgctttctct ttaaaattca cagtccctgt cgtagctgag ttagagatcg gctattgatg
    11461 tattatcaac ccttaacaaa aggaaattaa aatgtctcta agcgtagaag caaaagcaaa
    11521 aatcgttgct gaatttggtc gtgatgcaaa agacactggt tcttctgaag ttcaaattgc
    11581 attattaact gcacaaatca accacttaca agcacacttt gctgagcata aaaaagatca
    11641 ccacggtcgt cgtggtttat tacgtatggt ttcacgccgt cgtaaattat tagactactt
    11701 aaaacgtact gatcttgcta aatattcgga aactatcgca cgtttaggtt tacgtcgcta
    11761 atttcaaata ttagatatga gagccgagct gaaaagttcg gcttttttat aaattttatt
    11821 cgaggaaaaa atatggcttt tgcgtcatta tttacattat tagacgatat tgctgccgtg
    11881 cttgatgatg tatcgatcat gactaaagtg gctgctaaga aaacagcagg cgtaatcggt
    11941 gatgatcttg ccttaaatgc taatcaagtc agtggaaaga atgtggtgcc agaacgagaa
    12001 ctaccgattg tttgggctgt agcgaaaggc tcattaatca ataaagtgat cttgattcca
    12061 ctagcgttgc tgctatcggt ctttttaccg caggctatta tcccgctatt aatgataggc
    12121 ggtgcatatt tatgttttga aggcgtagag aaattactcc atcgtctatt acataaaaac
    12181 aatcaagctc attctgatga aaccgaaacc ctttctgaaa aagataaaat taaaggtgca
    12241 attcgcaccg attttattct atcagcagag attatcatta ttgcgttagg tgttttgcaa
    12301 gactcttcaa ccctaaccca aattttatct ctctccgcta tcggggtcgg aattaccctg
    12361 tttgtttatg gcttagttgg attaattgta aaactagatg atatcggttt atggttaatg
    12421 cagaaaaaaa gcacattctc acaagcggtt ggaaaaggct tattatttgc aatgccgtgg
    12481 tttatgaaag gactttctgt gattggtact attgcgatgt tcttagtcgg tggtggtatt
    12541 tttacccata atttaccaat gattcatcac ttcgtagaac aattcggtat ctctccccat
    12601 ttttctactc tggttgattt tgcagtgggt attgtggttg gtattattgc atgcttaatt
    12661 attttacctt tgatgaaatt attttctaaa aaggcgcact aatccccaaa agcgtagctt
    12721 tggttaaagc tagcttttta tctcttgcta taaaaaaata gcaggtttca agcctgctat
    12781 ttttttaatt attataatga gttagactca gtggctgttt gcggtaaggc taatggctca
    12841 ttaactttag actgagtagc catattcacc tgtttgagat tttcctcacg aatacggttt
    12901 gccattaatg tatcccccat ctgctctgcc acgtgcagtg ccataatacg atcatcagca
    12961 acacattcat catgagccaa taacaattca aactgagact tcgctttttg gtattgccca
    13021 tctcgtgtat aaatatagcc aagcgctcta gcataatcat cggtatattt aacattcgct
    13081 ttatctgccc gttttgctaa tacttttagt agcttactgt cagaatcaat ttgtaaacgg
    13141 gttaattgca caaataaacc ttgtaattgc tcatcttcaa atttcttcaa cgtttctaaa
    13201 gcaatttctt gtgcagattg atgatcgtca ctatcaatta aacgcttgat gacaccgaca
    13261 cgactataca ccgatttacg acgacgattt ggttggtttt cccaccaaga aagcaagcct
    13321 tcctgccctt cttcctgtaa acgctcatct tgtaaaccat catcaacgaa atactctaac
    13381 tcagcatact cttctgcagt taagaaatta cgttggccga tttcaactaa taatctatct
    13441 aacgcaacat aagctctgga ttctttataa atttgaatcg ctaagcgtaa tacctcatcg
    13501 ctatgaggtg caagctctag taggctatca atcgcagttc gggcagccgg taattttcct
    13561 tgttgtaata aaattcgggt tcgtaccaac tccactgcaa cattattcgg tccagctatt
    13621 tcagttgcct taattaaata tttatttgca gctaaatcat caccattttg ctgagcagct
    13681 tccgccgctt taattaaatt cagtacaggc tcatctgcat gttttgcatt tttactaaag
    13741 agtttttccg cttttgagta attcccttca ctcatacgca ttaaaccttc cagtgtttct
    13801 tgctgagctt ttttatgctt acgagttgag aaccagttat aagctccatt actcatacgg
    13861 catattttag tgacaatcca ttcaagtaaa taaactaccg ccattgccaa cacaaaaaaa
    13921 accacaagca ttacaatgct catttcaatc acggtagttg ctgtttcaat tctgacataa
    13981 ccttgatttc cggctaaata aggcccagag attagtccaa ctaataataa aagcatcaaa
    14041 aagagcgttc taaacataat ctattctcct ttttattatt gtgctgaaag aggcatcgca
    14101 tcttgctctg cttttacagg ctctgcttgc tctaattctt tttcagcttt tatttcgact
    14161 ttctctattt tttcaggggt tttattaagt tgctgagcaa tcacctttaa gctttgtaat
    14221 gaagttggag catccacata aatagtttgt tctgccaact catctagctc tttcaagaaa
    14281 tgttgcacat tttcatttga agtttcaaaa taacttctta cccaagagcc taccgtttgt
    14341 aacgattttt tataaagttc gctttgctgg cgaggaacag ctaaaatcgc aatttgtaaa
    14401 cgcaagcgaa tgttttcacg caaataaatt tcttgattcg gtgcaacaaa ggctttctca
    14461 tctgctgcat tacgtttcga tacacgaata aaatgatcta aaaatgaagc agcacttttt
    14521 tctaaatttt tctcccaatc cgcaagagaa tcacttactt gcccatcttg tttgctattt
    14581 gaagcttgtt cactttccaa aatcggcaaa tcatctaatt tattcgctaa atttgcgaca
    14641 cgctgcatta aggcattttg atcgatctga tgcaatgcgg ttaatgtatt taaatcagtt
    14701 ttgattgcct cacgaatgtg aatcgtattc ggtgcttttg ataaaacttg atctgcctct
    14761 aacaataaat ttttcgctgt ttcaatgtca ttatctaaca ccactttacg agccgcatta
    14821 ttcaatagga aatctgcctc agataatagc cagaatgtgt tatccgaacc agaggtatta
    14881 tttagctttt gcagttgaag ctgcaaacta ctaatttgct gggtataacc attttgggct
    14941 gtttctaaag ctttaatccg ttctaaagct ttttgatatt ccgtgaccaa ttcggcaatt
    15001 tgtgcttttt cattttcgaa atttggcatc tcaaccatag tttgagtagt aggctgattt
    15061 gctttagcag atactgcctg aatttgagat tcaacctctg caaacttttt atttgccata
    15121 taatgaccag ctccgccaac cgctaaagcc actaatagtg caactaatgc caagcccttt
    15181 ccgcccgatt tttgaacaac cacttgttct tttggcgctt tttgcatttc tgtttgaata
    15241 tctttctcta tagcttcgtt cactacatct tcctcaatct ctttttttgc aaaagatccc
    15301 gctgaatcga ccgcttgttg tgcctcttct accacctttt cagctgaatg ctctaaatgt
    15361 tcagttactt ttttagatct tgacatactc gccacctcta gttagatttg cccactttaa
    15421 ataataatgt attcatcaaa gttacattat cggctttatc cgatacccta atcttatctt
    15481 cttgccagcc catttgttta gctatctttg caatccgccg gctcactaca accagatcac
    15541 attcaaataa ccatgctcta caatctgctt tcgcttgttc ataaagtgat cttaaaattt
    15601 cactactggt gatcacaata gtatctactc ccaagcgttt acataagctg attttttcgg
    15661 gaatatcatc ggtaaccggc tctctacgat aacactccaa atattgaata gttgccccac
    15721 gcaagactgc agtttcagca agtagctcac gcccagaatc ggctcttaga attaaaatag
    15781 ttttatcagt tagattctgc atttcaggta atgccaaaac cccctcgcta ttttcaaatt
    15841 ctatcggata aatgaccgct tgttcagctt tttctgttaa atatttcgca gtccgttgcc
    15901 ctaccgcaaa atatttcaaa tctgaacgaa aatggaaccc ggtatctact aatgtttttg
    15961 cagcatagtc cactgcattc ggcgaaacaa caaatacata atcgtctgat tttagccgag
    16021 aaatagccga aggaagcaaa ggtaattctt taccggcttc aatcgtaaat agcggttgat
    16081 gaatagcaaa aatttgctgt tcattcaaca tatctaccaa ttcttgcccc cgactatcca
    16141 ggcgtgtgac caacacattc attattgtac gttttcgtta taaaccgcag ctaaaatttt
    16201 atctgcacct tgagcaagca actgttccgc caccaaaatg cctaattgct ccgcctcttg
    16261 cttatgtcct tttgccgaag cccgaataat ttgtgagcca tctaacgatc caactaacgc
    16321 attaagcgtt aattcatcgc cctctaatgt agcataacca ccaatcggca cttggcaacc
    16381 gccttgcaaa cgggtattca ttgcacgttc tgccatcacg caacaagcgg tcgaactatg
    16441 gtttaatttt gcaatgtagt tcaaaatccg ttcatcattg gctcgagtct caatccctac
    16501 tgctccttga ccagctgcgg gtaatgattg ttccacagaa atatagctac ggattcgctc
    16561 ttgcaagcct aaacgaatta agccggcaga ggctaaaata atcgcatcat actcgccatt
    16621 atctaatttg cttaagcgag tgccgacatt gcctcgcaat gatttaacct ctaaatgcgg
    16681 atatttcgcc attaattggc actgacggcg taagctcgat gtaccgacca cagcaccttt
    16741 cggcaaatct tctaacgaat ggtagttgtt cgacacaaac gcatcacggg gatcttcacg
    16801 ttcacaaatc accgcaagcc ccaagccttc gggaaagctc atcggcacat ctttcattga
    16861 atgcaccgca atatcagctc gattttctaa aagggctaat tctaactctt tcacaaacaa
    16921 gccttttccg ccaattttgg ctaatggggt gtctaaaata atatcgcctt tcgttaccat
    16981 cgtaaccagt tcgacagata attgcggaaa atgtttttct aattcgcttt ttacaaaatt
    17041 tgcctgccat aaggctaacg ggctttgacg ggttgcaatg cgtaaaatat ctttcataaa
    17101 ttctaaaatt aaaacattga gttaatgaat tatcctacca ctttttagct gaactgccaa
    17161 aaataatcgt ggatttcgct ttgcatttaa tcaaaaatcc gact
//
LOCUS       DANLZC010000043        17169 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.043, whole genome shotgun sequence.
ACCESSION   DANLZC010000043 DANLZC010000000
VERSION     DANLZC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17169
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.043"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..253)
                     /locus_tag="PXE44_001085"
     CDS             complement(<1..253)
                     /locus_tag="PXE44_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDL5588606.1"
                     /translation="MNELYEKYKDEPSMLVAIADGEGNIGVLEDIFLYKKQLAFSYVT
                     PLERWNWLHQCNLNSYAPAPLSVIEFYIQNGVEINAQDCY"
     gene            complement(255..9899)
                     /locus_tag="PXE44_001086"
     CDS             complement(255..9899)
                     /locus_tag="PXE44_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HDL5588607.1"
                     /translation="MNKHCFRTIFSKTLQRIVVVSELAKLAGKSESESMTSGAILQKI
                     CKIRPLAFSLFCALGFVTLSDNAMAETLIIQADSTAPKNQQPIILQTANGLPQINIQT
                     PNDKGLSHNKYSQFDVAEKGAILNNSRTNTATQQAGMVQGNPYLARGEAKVILNEVNS
                     SKPSVMKGYVEVAGKKAEVIIANPSGLHCDGCGIINADRATLTTGKPQINQGNLEGFV
                     VEKGKVTVAGKGLDNSRVDYTDILAREAKINAGVWSKKETKVITGKNTVKRSNSDNNL
                     QIIHTNQPLAGEEKPTVAIDVGELGGMYAGKIHLIGTEQGVGVRNAGHIGASADTLTI
                     DSQGRIVNSGTFNAQRQVQLNAQHGIDNQGKIENKQGNIQLNSKADIQNSGSIVARGG
                     NIQKKAETKIQQSGETVAKGNITYTARQIHADKNSLIAAGVDVTDTAQGEVRKLEAQS
                     VAGKTIQLTAPEKTTVQGKNIASGKINVAAAEIDANQSQNNAHTVEYSAKSGDIQANS
                     AQFSANQIKLTTPKWLSTEGSQLTAEVIEAQQANLNARGSVWKQTGEQDFRLKAESIH
                     TDSARFSVGGHFEIDGNRLTNNQGLLSSGKRLGLNLNDAVDSVDGKLVAKGELSIRAP
                     LLNNQQGLILSGEKQAITVNTLKNQSGVITSQTEQTLDITSELQNQGGRISASGVAIN
                     AQSLDNREQGEISSVKELALTVSQQVDNRHGVIQSVGNLTLNSTLLDNQGGVLKSATD
                     ITLNVPTVLNNRISENGSLIEAGGSLTMNSFEVENQGTLARQPQPIQGIIASQFVLNS
                     TKQLNNEKGGIYIGSSARFTLSDSLNNRNGEILSWGDLAIFGGKSLTMTNTEGKVSAN
                     NGLEITAKALSGDGDVQAGDIRLNLQENFDITKDINANRSMIIQTEGDILNRKKLAAN
                     DRLQLTANQITNAQNARISSADTRLTATSTVYNEGLINSRSDNDNAQTVIKAQEIHNI
                     GTGNIYGDKVALGAERILNQDKDGTSATIAARQSLGLAAKVIENNTLVYEANKKSGSL
                     IYSGGEIAIGRQLNEQDTVIGNAGQLHNRSSIIEAEGRIQLNADKVANVNSHYRSELE
                     TTDRQTVNEHFILPRDKHNRGGSDVYDNGHLPTINVNQLRKGYYRKTWSYWQKYDEAN
                     LPVITDISQLNATTVLAKPYAQSCANDESVDCTLLPAGNYKKDNPVWTWFNLTPPAED
                     APEMTPEIIQAMNEEDLANQHLSEAEQEKLQKEQGQGIAPVVPARPIEPVRGSTETEA
                     QYQKRLQTYQAELKAYEAAEAAYQRYVLLKPLIDWEAKYGANIQALDEAIAKHNKANL
                     SQEYPHFWNLWIKERMVKENITKASLPAQILAGGDLTYQSDEFINDKSWVIAGKALNP
                     IGRSRIANLDDADAVRQDWELGRRQFSFTEYHGGTRRRHSRYDENDGPLARLKEVHKD
                     MNIFVELENTSPTAYTDYVDAKAANAVKPEGNQVERGNLPDAPNPPTMAIERIPSPNA
                     AAFEVRSMTVDTRLPNQGLYRINPQADSHVLIETDPDFTDKKRWLSSDYMFNALRHDH
                     NNVHKRLGDGFYEQRLIREQINRLTGRQFLGDYTDFDSQYKALMDAGITFAQKFNLRL
                     GITLSPEQVASLTSDIVWFETQTVRLPSGETVSVLVPKVYALARKGDIDGKGSLISAD
                     ALTINSKEIVNQGTIAGRRLAQFNAERLENSGTLSGGVLAAKVSGDLENIGGKIEADK
                     AILLDIAGNFNHTSTTRTTQIDLDGFKRAETNLDRKALLHVKDENGLLQVQAGNITSA
                     GADIINDGQGQTYLSAKNQLNLTALQVGFDEKMGGGNHYRNEALNDVVISRVQGKGDV
                     VVSGKNLYSEGADLDAKARLALLAENDMVLGSATRSRDYEEYHKTKSGGALAKKTKTT
                     YDNISETEHKGTELTGEDILVKAGHDIQGESLLAVSRSGDIDIIGGNNVTLSSATNQL
                     SEQHIRETKRSGFLNGGEIGFTIGSQKTRQENSLEGAIQSTARNTLGSEGGNIRIQAG
                     NKASVSNVDVLISNEKTAVISGKNGVRVESGKDVINSTDNYEFSQSGLSIALSTPVTD
                     AVQSARQSVQKAKATQNEKLKGVYAVKAAEDAVIAAQNAQKVAETLGNLGNALTENHA
                     AAENPAVKISVSLGTQKQQRESRSQSVTHSKSTLNGGNIALISGAGKVALEGVDTRVK
                     DTLLLDGKLGIESKGVADTYQNNTKNKNHSASVGVFIGLDRDSFGIGFEAGVSGGKGK
                     ENIETETWQNNQLQAGKIVTNSANGKLMLDATNVKANHWEGEVQDFEARSRQDISKYK
                     SEYTEGSVHGTLAVGTTGVNGHVAYNGAKLNTAQVENQTAIDIGKSGMDVKVKKNAHF
                     EGAVMTSQAEKENNRFQAGTLTTGDIENHSELKTRSVAISGGTSGVNPMSALSLLGNK
                     NESERSTTKAAIGENIAITLTEDLNAETTLNHLNRDTQNANQKVTKYDISEVKETQEL
                     VKGIGEIAEKAYQIYTHSEREKVTEAKLELGKAQAQKASKEEIGKLEANLDRLQKEFD
                     KNYGTGSKTKRAVEAVTAALQGLAAKDVGQAAVGLASPYLNAEIKKYTEGDTQANLIA
                     HALLGAVEAAATGNNVLAGAAAGAGSEAAADFIVKTLYKGKSTQDLTEAEKQNVALLS
                     QLASGLASGLIGDSTQSAAVGADIGKRAVENNYLSNWQEQKRSQELKSCHGDARCENQ
                     VRLYWGLVDIGQDVSFVSGAVAGVPENLAESVEGLISMFSSPIETIVAMKELIAREDT
                     LSYIANMTKQEYLAKLDKMQVYYEQAGAVGSFNAGKEFGNMMTDVVTALTGAGALVKT
                     GVGTTAKIVTKSGKELAIPKTRSSASLGTCSFHGDMEVKTDKGYRQISSIKVGDKVLA
                     KNERTGITTYQKVQAHYSNPYDYTVYVEVADSKGKYHTIVSNKIHPFFTQVLSGRVPI
                     SSEGHSYNGEIAKAQWVDAQHLQKGYRLLAESGEWQTVTKVKIKQERLKAYNMTVEKD
                     HTYFIRGTNADNEGVWVHNDCWHALPNDAKQVKNIDGYKAYQFTDPKDGKVVTVVQKD
                     LNRFETPNHQAGVDPHFNRLSQQIDAETGRYLSTDNPVDGLYNRAYLRSETLQKIYAN
                     YEKLPNGNYRNKLTRQEIEQPIDIGHMYGWEHRRLSLAAKELNWSQKQFNDYVNARPE
                     NFRLENMSENRSHKNEMPGKHDIQRIKSDMEKFLKEGK"
     gene            complement(9951..11726)
                     /locus_tag="PXE44_001087"
     CDS             complement(9951..11726)
                     /locus_tag="PXE44_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL5588608.1"
                     /translation="MKTPQNLILSTVLLSASFVAEANSSSLVQQARIDATQQQIDAAQ
                     QHRQQQYQQNQAEKFQPKADVRLETQINDTLTLSNHELPCYPIHQITLIDYSPDNSLT
                     TSQFQWAFEKAVKSFHLTLPHCFGGEGLAVLMKQIQNEIIGKGYVTTRVVTAEQDLRG
                     GKLVLTVIPGKIRNTLVVDSGKVPRFTKLHALTGFTFAEGDILNVRDIEQSLENLKRV
                     PTAEANIEILPSSSEKAEVGDSDLKISYSQAFPFRLNLGLDDAGSKSTGKLQTSATLS
                     IDNIFSANDLFYTSFTHSMKQKGDDNGRRASKNLTLYYSVPFGYWQLAFSHTHHRYHQ
                     EVFGAFDKSYLYAGESDTDKLTFSYLLYRDAKRKTSLSGSLWPRQSQNYIDGSEIEVQ
                     KRRMAGWEAGFSHKEYFPTATLELSANFKRGTAARGALSAPEEDYGEGTSRPKIITAS
                     ASLTKPFFWGKQPWQWQSSLNAQWNKTPLVPQDRFSIGGRYTIRGFDGELTLSGERGW
                     NWRNELSWQFNPNHAFYWAVDGGRVSGWATENQLGHHLMGTAIGFRGGVKGFSYDVFA
                     GRPVRKPEGFKTSDTVAGFNIGYNF"
     gene            11881..12966
                     /gene="queA"
                     /locus_tag="PXE44_001088"
     CDS             11881..12966
                     /gene="queA"
                     /locus_tag="PXE44_001088"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL5588609.1"
                     /translation="MLVSDFHFNLPDELIARYPTAERSASRLLHLNGETGEFADQQFV
                     DLYDHINEGDLLIFNNTRVIPARLYGRKPSGGKVEALVERVLDEHRCLAHVKASKAPK
                     EGAELIFGEDKLGEGKGFTATMVARHDTLFELVFNEEQPLFELLQQAGHMPLPPYIDR
                     PDEDADQERYQTVYSKVLGAVAAPTAGLHFDNAMLEKLKAKGVDTAFVTLHVGAGTFQ
                     PVRVETIEDHKMHAEYAEVSQEVVDKILATKAKGKRVIAIGTTSVRSVESAAQAAEKD
                     GKLIAPFFSDTAIFLYPGKTFKVIDALVTNFHLPESTLIMLVSAFAGYRNTMNAYAHA
                     VETKYRFFSYGDAMFINKNPNALSDVP"
     gene            complement(12993..13531)
                     /locus_tag="PXE44_001089"
                     /pseudo
     CDS             complement(12993..13531)
                     /locus_tag="PXE44_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13544..15826)
                     /locus_tag="PXE44_001090"
     CDS             complement(13544..15826)
                     /locus_tag="PXE44_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL5588610.1"
                     /translation="MTLDRFCIALILFHLPILWLPKAFLFVGIVIGTLVLILSVYLRS
                     KLTAVLGLLVCIGYIQIIQVAKNAEKTTASKTPVQFEISKILKQTDYQTAIATLNTGE
                     KIYLNWQSKQPLLLNQFYQAELALRPISARSNIGNFDRQRWYFANHINMTATVRKVET
                     LAKTDYSLRTNWLNKVKAETDALPTQGFLLALAFGERAWLKTEHWGQFQQTATAHLIA
                     ISGLHIGLAMLFGVYFAKGVQWLCLKSELTALQAVGFSLYFARIVSFITAFGYSYLAG
                     FAVPTARALLAIAFVLLCQVLRRHYTPWQYWGRIVALLILIDPLSLLSDSFWLSILAV
                     ASLILWYQYFPLSRFLNDEYRKKITKFNRLWLSLLHLQIGIWLIFSPVQLYFFEGISA
                     FALLANLIIVPLYSFLLVPLILFTLLTDNLFSTWLLADYLAQFSLWLLDPFSNAWFAL
                     SHWQQWQLLSLNLLVLVLLYCKLYRLSYKKWLSAVAVSLLFNLSFYLPKLFNQTQTEW
                     IMFDVGQGLAMAFIYQQNKAVIYDTGSSWQANDGSRNSMAKMEILPYLKRNGIEVEAI
                     FLSHDDNDHSGGVVELLQAYPKARLISSSKVTYHNINPEPCIAGTSWQFGEWQLLAIY
                     PSHIAERAKNQDSCVILAKNDRLKILLTGDSGVEQERQFAHQLGKIDFLQVGHHGSKS
                     STGETLLSITQPAFAVISAGRWNPWKLPNKQIIERLNRRHINVLNTVETGMIRVKFYP
                     SSIEIEQARGAFSPWYKQFY"
     gene            complement(15968..17158)
                     /gene="sstT"
                     /locus_tag="PXE44_001091"
     CDS             complement(15968..17158)
                     /gene="sstT"
                     /locus_tag="PXE44_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL5588611.1"
                     /translation="MLGGNLVLRIAIGLVLGVLLAFINKEWATSVGVLGQFFVKSLRA
                     IAPILVFVLVLAAIANKEVGSDSKLKPILVLYVLGTFFAALTAVVLSFMFPTTLELTA
                     SPDGLTPPQGIGEILKTVIFNLVDNPLVALSNANFIGILAWSIGLGIAFRHGSASSKA
                     FLNDLANAVSFVVKVVIAFAPIGVFGLVAETVAINGIDAFEDYARLLVVLVGAMLFVA
                     LVLNPLLVYWKIRRNPYPLTFTCLRESGVTAFFTRSSAANIPVNMNLAKRLGVRDEIA
                     SVAIPLGASINMAGAAITVTVLTLAAAYTQGIQPDFATALLLSVVASICACGASGVAG
                     GSLLLIPLACSLFNIPNDIAAQVIGVGFVIGVIQDSVETALNSSTDVLFTAAVSYAED
                     QKSA"
BASE COUNT         4412 a         4033 c         3420 g         5304 t
ORIGIN      
        1 cgtagcaatc ttgggcattt atttccactc cattttgtat ataaaattca atcacagaaa
       61 gtggtgctgg tgcataagaa ttaagattac attgatgtaa ccaattccat ctttctaatg
      121 gtgtaacata agagaatgct aattgctttt tatataaaaa aatatcttct aatactccaa
      181 tgttaccttc gccatctgca atggcaacta gcattgaggg ttcatcttta tatttttcat
      241 ataattcatt catattattt accttctttt aagaattttt ccatatcgga tttgattcgt
      301 tgaatatcat gttttcctgg catctcattc ttatggctac gattttcaga catattttct
      361 aatctgaaat tctccggtct cgcattaaca taatcattaa attgtttttg actccaattt
      421 aattcttttg ccgctaatga taatcggcga tgttcccagc catacatatg acctatatca
      481 ataggctgtt caatttcttg acgggttaat ttattgcgat aattaccatt tggcaatttt
      541 tcataattag cataaatttt ctgtaacgtt tccgagcgta aataagctcg gttatatagc
      601 ccatctaccg gattatctgt cgataaatag cgtcccgttt cggcatcaat ttgctgagac
      661 aaacggttaa aatgtggatc taccccggct tgatggtttg gcgtttcaaa acgattgaga
      721 tctttttgaa ctactgtcac cactttcccg tctttcgggt ctgtgaactg ataagcttta
      781 tagccatcaa tattttttac ctgtttggca tcattcggta aagcgtgcca acaatcatta
      841 tgcacccata ccccttcatt atcggcattt gtacctctaa taaagtaagt atgatctttc
      901 tctaccgtca tattgtaggc ttttaatctt tcctgtttga tttttacctt agtgacagtc
      961 tgccactcac cactttcagc aagtaagcga tagccttttt gcaaatgttg agcatcaacc
     1021 cactgagctt ttgctatctc tccgttgtaa gaatgcccct ccgaagaaat cggcactctg
     1081 cccgataata cttgcgtaaa gaacgggtgg attttgtttg acacaatggt gtggtatttt
     1141 cctttgctgt ctgcgacttc tacatacacc gtataatcgt atggattact gtaatgagct
     1201 tggactttct gataggtggt tataccggtg cgttcatttt tcgccagcac cttatcgccc
     1261 actttaatgc tggaaatctg tcgataacct ttatcagttt tgacttccat atcaccgtgg
     1321 aatgagcaag tgcctaaact agcgctcgat ctggtcttag gaatcgcaag ttctttacct
     1381 gatttggtga ctattttcgc cgttgtccct acacctgttt taacaagagc accagcaccg
     1441 gttaatgctg tcaccacatc agtcatcata ttgccaaact cttttccggc attaaaagaa
     1501 cctacagccc ctgcctgttc atagtaaacc tgcattttat cgagtttagc taaatactcc
     1561 tgtttagtca tattagcaat ataggagagc gtatcttctc ttgcgatcaa ttctttcatc
     1621 gccacaatag tttctattgg gcttgagaac atggagatca atccttcaac gctctcagcg
     1681 agattctcag gaacaccagc aacggcgcct gatacaaaac taacatcttg cccaatatca
     1741 actaatcccc aatataatcg aacttggttt tcacagcggg catcaccatg gcaggatttt
     1801 aattcttgtg agcgtttctg ctcctgccag ttcgagaggt agttattctc cactgccctt
     1861 ttcccaatat ccgccccaac cgctgccgat tgtgtactat cccctatcaa acctgaagcc
     1921 agtccgcttg caagctggct tagcaacgcc acattctgtt tctcggcttc ggttaagtct
     1981 tgcgtggatt tgcccttgta gagggtttta actataaagt ccgctgccgc ttcgctccct
     2041 gcgcctgcag cagcccctgc cagtacattg ttccctgtcg cagccgcttc caccgcaccc
     2101 agtaaggcgt gggcaatcag gttggcttgg gtatcccctt cggtgtactt tttgatttct
     2161 gcatttaagt atggtgaggc taaacccact gccgcttgtc ccacgtcttt cgccgctaaa
     2221 ccttgcaagg ctgccgtcac cgcttccacc gctcgtttgg ttttactgcc cgtgccgtag
     2281 tttttatcaa actctttttg caagcggtct aaattggctt ctaatttacc aatctcttcc
     2341 ttgctcgctt tttgagcttg tgctttgccc aattccagct tcgcttccgt cactttttca
     2401 cgctcactat gcgtgtaaat ctgataagcc ttttctgcaa tctcgcctat gcctttcacc
     2461 agctcttgtg tttctttcac ctcactgata tcgtatttcg tcactttttg attggcgttt
     2521 tgcgtatctc ggttgagatg attaagcgta gtttccgcat ttaggtcttc ggtgagggtg
     2581 atagcgatat tttctccaat cgctgccttt gttgtgcttc gctcgctttc gtttttattg
     2641 cccagtaagc tcagtgccga catcgggttc accccacttg tccctccgct aatcgcgaca
     2701 cttcgggttt tcaattcact gtggttttca atatcgccgg tggtcagcgt gccggcttgg
     2761 aagcggttgt tttctttctc cgcttggcta gtcattaccg ctccctcaaa atgggcgttc
     2821 tttttcactt taacgtccat tccactcttg cctatgtcga ttgcagtttg gttttccacc
     2881 tgagcagtgt tcaacttcgc tccattatat gccacatgac catttactcc tgttgtccct
     2941 accgccaatg tgccatgtac gctgccttct gtgtattcgc ttttatattt gcttatatct
     3001 tgtcgacttc ttgcctcaaa atcttgtacc tcaccttccc aatgatttgc tttgacattg
     3061 gttgcatcca gcatgagttt accatttgca gaatttgtaa caattttacc cgcttgcagt
     3121 tggttgttct gccacgtttc agtttcgata ttctccttgc ctttacctcc actcactcct
     3181 gcctcaaaac caataccaaa actatctcta tctaaaccga taaatacgcc cacactcgca
     3241 ctgtgattct tattcttagt gttgttttga taagtatccg caacaccttt ggattcaata
     3301 cccagcttac cgtcaagcag cagggtgtct ttcactcggg tatccacccc ttccagcgca
     3361 actttgcccg ctcccgaaat caaggcaata ttgccgccgt tcagggtgct tttgctgtgg
     3421 gtcacgcttt gactacggct ttctctttgc tgtttttgcg tgccgagact gactgaaatc
     3481 ttcaccgccg gattttctgc cgcagcatgg ttttctgtga gtgcattgcc aagattgccc
     3541 agcgtttctg ccactttctg agcattttgt gctgcaatca cggcatcctc cgccgctttt
     3601 accgcatata cccctttgag tttctcgttt tgtgttgcct tcgctttttg caccgattgc
     3661 cgtgccgatt gcaccgcatc cgttaccggt gtactcaacg caatgcttaa gcccgactgg
     3721 ctaaattcat aattatcggt gctgttaata acatctttcc ctgattcaac ccgtaccccg
     3781 tttttgcctg aaatcacggc ggttttttcg ttggaaatca gcacatcgac attgctcacg
     3841 ctcgctttat tgccggcttg aatccgaata ttgccccctt cactgcccaa tgtattgcgt
     3901 gccgtgcttt ggattgcccc ctctaagctg ttctcttggc gtgttttttg gctaccgata
     3961 gtaaagccga tttcaccacc gttcaagaaa ccgctgcgtt ttgtctcacg aatatgctgt
     4021 tctgaaagtt ggtttgttgc cgaactgagc gttacattat taccgccgat aatatcaata
     4081 tcgccactgc gtgagacggc aagaagactt tcaccttgaa tatcgtgacc ggctttcact
     4141 aaaatgtctt caccggttag ttcagtgcct ttgtgttcgg tttcagaaat attgtcataa
     4201 gtcgtttttg ttttcttcgc tagcgcacca ccactttttg ttttgtgata ctcttcataa
     4261 tcgcgagaac gtgttgcgct acccaacacc atatcatttt cagccagtaa cgctaaacgc
     4321 gctttggcat ccaaatccgc tccttcactg taaaggtttt tccctgaaac caccacatcg
     4381 cctttgcctt gcacccgtga aatgaccaca tcattcaagg cttcattacg atagtgattg
     4441 ccaccgccca ttttttcatc aaaaccaacc tgtaaagcgg tcaaattcaa ctgatttttt
     4501 gcagataagt aggtctgccc ctgtccgtca ttgatgatgt ctgcgcctgc tgaggtgatg
     4561 ttgcctgcct gtacttgcag cagtccattt tcatctttaa cgtgcagtag ggctttgcgg
     4621 tcaaggttgg tttcagcgcg tttgaaaccg tctaggtcaa tctgtgtggt tcgagtggtg
     4681 gaggtatggt taaaattgcc cgcaatatcc agcaaaatcg ctttatccgc ttcaatcttg
     4741 ccgccgatat tctctagatc accgctcact ttggcagcaa gcacgccacc actgagtgtg
     4801 ccactattct ctaaccgttc ggcattaaac tgtgctaaac ggcggcctgc tatcgtgcct
     4861 tggtttacaa tctctttact gttgatggta agcgcatcgg ctgaaatcag gctgcctttg
     4921 ccgtcaatat cacctttgcg tgcaagggca tacactttcg gcactaatac cgataccgtt
     4981 tcaccgcttg gcaagcgaac cgtttgggtt tcaaaccaaa caatatcgct ggtcaagctt
     5041 gccacttgct ccggcgataa ggtaatgcct aaccgaagat tgaatttctg cgcaaaggtg
     5101 atgccggcat ccatcagggc tttatattgg ctatcaaaat ccgtatagtc gcccaaaaat
     5161 tgtctgccgg ttaagcggtt gatttgctct cgaattaagc gctgttcata aaaaccatcg
     5221 cccaaacgtt tatgcacatt gttatggtcg tggcgtaggg cattgaacat ataatcactg
     5281 cttaaccagc gttttttatc ggtgaaatcc ggatcggttt cgatcagaac atggctatcc
     5341 gcttgagggt taatgcgata aagcccttgg tttggcaaac gggtatcgac cgtcattgag
     5401 cgaacctcaa atgcggctgc attcgggctt ggtatgcgtt caatcgccat tgttggcgga
     5461 ttaggggcat cgggtagatt accccgttca acctgattgc cttccggctt cacggcattt
     5521 gctgctttag cgtccacata atctgtataa gcggtcggtg aagtattttc cagctcgaca
     5581 aaaatattca tatccttatg gacttctttt aaacgagcga gaggaccatc attttcatcg
     5641 tagcgggagt ggcgacgtct tgtgcctccg tggtactcag taaaagagaa ttggcgcctt
     5701 cccagttccc aatcttggcg aacagcatct gcatcatcaa ggttggcaat tctactgcga
     5761 ccaatcgggt ttaacgcttt gcctgcaata acccaacttt tatcgttgat aaattcatcg
     5821 ctttgataag tgagatcgcc acctgccaag atctgtgccg gtaagctggc tttggtgata
     5881 ttctctttta ccatacgctc tttaatccac aaattccaga aatgcgggta ttcttggctt
     5941 aaattcgctt tattgtgctt tgcaatcgct tcatctaatg cttgaatatt ggcaccatat
     6001 tttgcttccc aatcaattaa cggttttaat agcacataac gctgataagc cgcttctgcc
     6061 gcttcatagg ctttcagttc cgcttgatat gtttgtagtc gtttttggta ttgggcttcc
     6121 gtttccgtag agcctctaac aggttcaatc ggtcttgccg gaaccaccgg tgcaatgcct
     6181 tgcccttgtt ccttctgtaa tttttcctgc tctgcctcac ttaaatgctg attcgctaaa
     6241 tcttcttcat tcattgcttg aataatttca ggcgtcattt ccggtgcatc ttcagctggt
     6301 ggtgttaaat taaaccacgt ccatactggg ttatcttttt tataattacc tgcaggtaat
     6361 agagtacaat ctacactttc atcattcgca cagctttgag catagggttt tgccaatacg
     6421 gtcgtggcat tcagctggct aatatcggta attaccggca agtttgcttc atcatacttt
     6481 tgccaatagc tccacgtttt gcgatagtag cctttgcgga gttgattcac attaattgtc
     6541 ggcaaatgcc cgttatcata tacatcactt ccacctcgat tatgcttatc tcttggcaaa
     6601 ataaaatgtt cgttcacagt ttggcgatca gtggtctcca attccgaacg ataatgacta
     6661 tttacattcg ctaccttatc cgcattcaac tgaatacgcc cttcagcttc aataatactg
     6721 ctgcggttat gtagctgccc tgcgttgcca ataacggtat cttgctcatt taattgacgt
     6781 ccaatcgcta tctctcctcc gctatatatt aatgaaccgc ttttcttatt cgcttcataa
     6841 actagggtat tattttcaat tactttagcc gctaaaccga gagattgacg agcagcaata
     6901 gtggcagagg tcccgtcttt atcttgattt agtatcctct ccgcacctaa cgccacctta
     6961 tcgccataaa tattccctgt gccgatattg tggatttctt gagctttaat cacagtttga
     7021 gcgttatcgt tgtccgagcg gctattgatt aagccttcgt tatagacggt tgaagttgca
     7081 gtcaaacggg tatctgccga gcttattcgg gcattttgtg cattggttat ttggtttgcc
     7141 gtgagctgca agcggtcatt tgcggctaat tttttgcgat tgagaatatc gccctcagtc
     7201 tgaataatca tcgacctatt cgcattgata tctttggtaa tatcaaagtt ctcttgcaaa
     7261 ttcaggcgaa tatcgcctgc ttgtacatca ccatcaccgg ataacgcttt ggcagtgatc
     7321 tccaaaccat tatttgcaga aacttttccc tcggtattcg tcattgtgag tgattttccg
     7381 ccgaaaatag ccagatcgcc ccaacttaaa atttccccat tacggttatt taagctgtcg
     7441 ctgagggtaa atcgtgctga tgagccgata taaataccgc ctttttcatt attgagctgt
     7501 tttgtgctgt tcagtacaaa ctgcgatgca ataatcccct gtattggctg aggttggcgt
     7561 gccaaggtac cttgattttc cacctcgaaa ctgttcatcg taagtgagcc acctgcttca
     7621 ataagtgagc cattttccga gatccgatta tttaagacag tcggtacatt gagcgtgata
     7681 tctgtggccg atttcagtac cccaccttgg ttatccaaaa gcgtgctatt tagggtgaga
     7741 tttcccacac tttgaataac accgtgccga ttatctactt gttgggaaac cgtgagcgct
     7801 aactctttta ctgaactaat ttctccctgc tcacggttat ctaaggattg agcgttaatc
     7861 gcaacaccgc tggcggaaat tcgcccgcct tgattttgca gttcgctagt tatatcaagg
     7921 gtttgctccg tttgagaggt gatcaccccc gattgatttt ttaacgtatt gaccgtaatc
     7981 gcctgttttt cgccactcaa aatcagacct tgctgattat tcagcaatgg agcacgaata
     8041 gataactcgc cttttgcaac caatttgcca tcgactgaat ccacagcatc atttaaattt
     8101 agccctaaac gcttgccact acttaatagc ccttggttat ttgttaaacg attaccgtca
     8161 atctcaaaat gtccacccac tgaaaagcga gccgagtcag tatggatact ctctgctttt
     8221 aggcgaaaat cctgctcacc agtctgcttc caaaccgagc cacgagcatt taaattcgct
     8281 tgttgggcct caatcacctc tgcggttagc tggcttcctt cagtggaaag ccattttggg
     8341 gtggtgagct tgatctgatt tgcactaaac tgcgcactat tcgcctgaat atcgcccgat
     8401 tttgcactgt attcaacggt atgggcatta ttttggctct gattggcatc aatctcagct
     8461 gccgccacgt taattttccc cgaagcgata ttttttccct gcacagtggt tttttctggg
     8521 gcagtgagtt gaatagtttt ccctgccaca gattgagctt ccagcttccg tacttcacct
     8581 tgtgcagtgt cggttacgtc caccccagcg gcaatcagtg agtttttgtc ggcgtgaatt
     8641 tggcgggcgg tgtaggtgat attgcctttt gcgaccgttt cgccactttg ctggatttta
     8701 gtttcggctt ttttctgaat attgccaccc ctcgccacga tggaaccgct attttgaata
     8761 tcggctttcg agttaagctg aatattgccc tgtttatttt caattttgcc ttggttgtca
     8821 atcccgtgtt gggcattgag ttgcacttgt ctttgagcgt tgaacgtgcc gctgtttaca
     8881 atccgccctt gactgtcaat ggtgagcgta tcggcacttg caccgatatg cccagcgtta
     8941 cgcacgccca cgccctgttc cgtaccaatc aggtggattt tgccggcata cattccgccc
     9001 agctcaccca catcaatcgc aactgtgggc ttttcttccc ctgcaagcgg ttgatttgta
     9061 tgaataattt gcaaattgtt gtccgaattt gaccgcttga cggtgttttt gccggtaatg
     9121 actttagtct cttttttcga ccagacaccg gcgttgattt tcgcttctcg ggcgaggata
     9181 tcggtgtaat ccactcggct gttgtccagc cctttgccag caaccgttac cttgcctttt
     9241 tccactacaa aaccttctaa attgccttga tttatctgtg gttttccggt ggtcagcgtg
     9301 gctcggtcgg cgttgataat accgcagccg tcacagtgca agccgctcgg gttggcgata
     9361 atcacttccg cttttttgcc agccacttcc acataacctt tcattaccga aggcttactg
     9421 gaattgactt catttaaaat caccttcgct tcgccacggg caagatacgg gttgccttgc
     9481 accataccgg cttgttgggt ggcggtgttg gtgcggctgt tgttgagaat tgcccccttc
     9541 tccgctacgt caaattggct gtatttattg tgggaaagcc ctttatcgtt cggggtttga
     9601 atattgattt gtggcagccc gttggcggtt tgcaaaataa tcggttgctg atttttcggg
     9661 gcagtgctat cggcttgaat aatcaaagtt tccgccatag cgttgtctga taaggtaacg
     9721 aatcccaacg cacagaacaa gctgaacgca agcggtcgaa ttttgcaaat tttttgtaaa
     9781 attgcaccgc ttgtcatact ttcactttct gatttacctg ctaattttgc taattcagat
     9841 accaccacaa tacgttgtaa ggtcttgctg aaaatagtgc gaaaacaatg cttattcata
     9901 ataattcctt aatagcacaa tatacttaat acaatatgcc caatcattca ttaaaaattg
     9961 taaccaatat taaaaccggc aacggtgtct gaggttttaa acccttccgg ttttcgcacc
    10021 ggtctaccgg caaacacgtc ataactaaat cctttaactc caccccgaaa cccaatggct
    10081 gttcccatta aatgatgtcc gagctgattt tccgttgccc aaccggacac tcgaccacca
    10141 tctaccgccc aataaaaagc gtggttcgga ttaaattgcc agctcaactc attacgccag
    10201 ttccagcccc gctcaccgga aagagtcagt tcaccgtcaa atccacgtat ggtgtaacgc
    10261 ccgccaatcg aaaaacggtc ttgcggaacc agaggggttt tattccattg ggcatttagg
    10321 ctactttgcc actgccatgg ctgcttgccc caaaaaaacg gtttcgtcag gcttgcactt
    10381 gccgtgatga ttttagggcg agaagtccct tcgccataat cttcctcggg agcagataat
    10441 gcccctcgtg ctgccgtacc tcgtttgaaa ttagccgaaa gctcaagcgt agcggtcggg
    10501 aaatattctt tatgcgaaaa acccgcttcc caacctgcca ttcggcgttt ttgtacttca
    10561 atttctgagc cgtcaatata gttttgcgac tggcgtggcc aaagtgaacc ggacaagctc
    10621 gttttacgtt tggcatcacg ataaagcaaa taagaaaagg ttaatttatc ggtatcactt
    10681 tcaccggcgt agagatagct cttgtcaaag gcaccaaata cttcttgatg ataacgatga
    10741 tgagtgtgtg aaaatgctaa ctgccaatag ccaaacggca cggaatagta aagcgtgaga
    10801 tttttactgg cacgtctgcc gttatcatca cctttttgtt tcatcgaatg ggtaaaagag
    10861 gtatagaaca ggtcatttgc cgaaaaaata ttatctatag acagagtggc ggaggtttgt
    10921 aatttgcctg tggatttcga gccggcatcg tccagcccaa gatttaagcg aaatggaaac
    10981 gcttgcgaat agctgatttt taaatcactg tctcccacct ccgctttttc gcttgagctg
    11041 ggtaaaatct caatatttgc ctccgccgtc ggcacccgtt ttagattttc cagagactgc
    11101 tcaatatctc gcacattcag aatatcgcct tcggcaaaag taaaaccggt taaggcgtgc
    11161 agcttggtaa aacgcggtac tttgccgcta tctaccacta aagtattgcg tattttgccc
    11221 ggaatcacgg ttaaaactaa ttttccgcca cgtaaatctt gctccgcagt aaccactcgt
    11281 gtagtgacat aacctttgcc aataatttca ttttggattt gcttcatcaa cacagctaac
    11341 ccttcaccac caaagcagtg aggtaatgtc aaatgaaatg attttactgc tttttcaaac
    11401 gcccattgga attggctggt agtcagagaa ttgtcaggag aatagtcaat gagagtgatt
    11461 tgatgaatgg ggtaacaagg caattcgtga ttggaaagag taagtgtatc attgatttgt
    11521 gtctcgagcc gtacatcagc tttcggctgg aatttttctg cttgattttg ttggtattgc
    11581 tgttggcgat gttgctgggc agcatcaatt tgttgttgag ttgcatcaat gcgtgcttgt
    11641 tgtacaaggc ttgaagaatt tgcttcagca acaaaagaag cacttaacaa taccgtgctt
    11701 aaaatcagat tttgtggggt tttcataaga tcaaataagt aagacattca acgtttttag
    11761 tctataaaaa tttatgttgt tttgcaaact ccgttttatg gagctttata catatctgac
    11821 gactttggtt ttttatgcta ttatctccgc catttttata ctatagagaa tagaataacc
    11881 gtgttagttt cagattttca tttcaactta cccgatgagc tgattgctcg ctacccgact
    11941 gccgaacgct ctgccagccg tttattacac ttaaatggcg aaacaggcga atttgccgat
    12001 caacaatttg tggatttata cgatcatatc aacgaaggcg atctattgat cttcaataac
    12061 actcgagtga tcccggctcg cctttacggt cgaaaaccta gtggcggcaa agtagaggca
    12121 ttagtggaac gggtgctaga tgaacaccgc tgcttggctc acgttaaagc ctcaaaagca
    12181 ccgaaagaag gggctgaatt gatctttggg gaagataagc taggtgaagg caaaggcttt
    12241 acggcgacaa tggtagctcg ccacgatact ctgtttgaat tggtgtttaa cgaagagcaa
    12301 ccgttattcg aattgctaca gcaagctggt catatgcctc ttccacctta tatcgaccgc
    12361 cccgatgaag atgctgacca agagcgttat caaaccgttt atagcaaagt gttaggtgca
    12421 gttgccgccc ctaccgccgg tttgcatttt gacaatgcaa tgcttgaaaa actaaaagca
    12481 aaaggggtgg atactgcctt cgtaacattg cacgttggtg ccggcacttt ccagccggta
    12541 cgggtagaaa ccattgaaga tcataaaatg cacgccgaat atgccgaagt gagccaagaa
    12601 gtagtcgata aaattctcgc aacgaaagcc aaaggaaaaa gagtgattgc aattggtacg
    12661 acatcagtac gttcggttga aagtgccgcc caagcggctg aaaaagatgg gaaattgatt
    12721 gctccgttct tttctgacac cgctattttc ctttacccgg gaaaaacctt taaagtcatt
    12781 gatgcgttag tcaccaattt ccatttaccg gaatccacgc taattatgtt agtctcagca
    12841 tttgccggct atagaaatac gatgaatgcc tacgcccacg cagtagaaac taaataccgt
    12901 tttttcagct atggcgatgc gatgtttatc aataaaaacc caaatgcgtt gagtgatgtt
    12961 ccttaattta tgagggttat aagggaataa cattatgaac aaaaacaatc ttccaaccat
    13021 tttaagggat tgttttcaat atattcaata atttggaagt acgatgtttc attacgaata
    13081 atgtgttcat aataattcct ttgccaaatt tcagaaatat cggtattttg acgaacccat
    13141 ttagttacgc caattttaaa accacgcaca atagagccga ttgttcgtga tgttccccgt
    13201 aagggcgaaa catttttcgc ccttatacat ggggcaaatg atgatttgcc cctacagttt
    13261 cgacgatttc aataatgcca tgtaaatgat taggcataat cacaaagcta tgcaagctta
    13321 catttggaaa atgatcgggt atatcttccc aacattgacg tacaatattc cctatcttat
    13381 taagcctcat ttcatcatca acaatctcac cgagaaaatg gtgtttattt ttacaacaaa
    13441 tagtaatgaa atagaatcct tgtgatcggt aatcataatg ctttagacga attgaacgcc
    13501 tcctatgaat ctccggctga tatttctgca tttttcttat tccttaataa aactgcttat
    13561 accaagggga aaatgccccc cttgcctgct caatttcaat agaacttggg taaaatttta
    13621 ctcttatcat tcccgtttct acggtattta atacattaat atgtcttctg tttaatctct
    13681 caataatctg tttattcggt aatttccatg ggttccatct tccagcagaa atcactgcaa
    13741 atgctggctg ggtaatcgat aataaagttt cgccagtgct gcttttgcta ccgtgatgtc
    13801 caacctgtag aaaatcaatt ttgccgagct gatgggcaaa ttggcgttct tgttccacgc
    13861 cagaatcccc tgtcagtaat atcttcaagc ggtcgttttt ggctaaaata acgcaagagt
    13921 cttggttttt agctcgttct gcaatgtgtg acgggtaaat tgccaataat tgccattcgc
    13981 caaattgcca cgatgttccg gcaatacaag gctcgggatt tatattgtga tacgtcacct
    14041 tgcttgaact aattaacctt gcttttgggt atgcttgcaa taactccact accccacctg
    14101 aatgatcatt atcatcgtga cttagaaaaa tcgcctctac ctcaatgcca tttcgcttta
    14161 aatacggcaa gatttccatt tttgccattg aattgcgact accatcattt gcttgccaac
    14221 tggagccggt atcgtaaatt accgctttat tttgttgata aatgaacgcc attgccagcc
    14281 cttgcccaac atcaaacata atccattctg tttgggtttg attaaagagc ttagggagat
    14341 aaaaactcag attgaacaat agggataccg ctactgcact taaccacttt ttataagata
    14401 gccgatataa tttgcaatac agcaatacta aaaccaataa attcagtgaa agtaactgcc
    14461 actgttgcca atggcttaat gcaaaccacg cattagaaaa tggatctaac agccataggc
    14521 taaattgagc aagatagtcc gccaacagcc acgttgaaaa cagattgtcg gtcaagagcg
    14581 taaataaaat aagcggtaca agcaaaaagc tatacaacgg cacaataatc aggttggcaa
    14641 gcagtgcaaa agcagagatg ccctcaaaaa agtagagttg aaccggtgag aaaatcagcc
    14701 aaatgccgat ttgtaagtgc aaaagcgata accacaagcg gttaaatttt gtgatttttt
    14761 tacgatattc atcgtttaaa aatcggctaa gaggaaaata ttgataccat aaaattaaac
    14821 ttgctaccgc taaaattgag agccaaaaac tatcagaaag caggctcaat gggtcaatta
    14881 aaatcagtaa cgccactatt ctcccccaat attgccaagg ggtatagtgt cgccgcagaa
    14941 cttggcaaag caagacaaac gcaatcgcca gcaacgctct ggcggtcggc acggcaaaac
    15001 cggctaaata gctgtaacca aatgcggtga tgaagctaac tattcttgca aaataaagtg
    15061 aaaatccgac cgcttgtaat gcggtaagct cgcttttcaa gcaaagccat tgcacgcctt
    15121 tggcaaagta aacaccaaac aacattgcca gcccgatatg caagcctgaa atggcaatta
    15181 gatgggcagt agcggtttgt tggaattgcc cccagtgttc tgttttcagc cacgcccttt
    15241 caccaaaagc cagagccaat aaaaagcctt gcgtaggcaa agcgtccgtt tcagctttca
    15301 ctttattcag ccaatttgtt cgcaagctat aatcagtttt ggctaatgtt tccaccttgc
    15361 gaaccgttgc tgtcatattg atatgattgg caaaatacca acgctgtcgg tcaaaattac
    15421 cgatattgct acgagctgaa ataggacgaa gagccagttc ggcttgatag aattgattaa
    15481 gtaacaacgg ctgtttcgat tgccagttca agtagatctt ctcgcctgta tttagtgtcg
    15541 caatcgctgt ttgataatcg gtttgtttta agattttact gatttcaaat tgaacaggcg
    15601 ttttactagc agtcgttttt tccgcatttt ttgcaacttg aatgatctga atatagccaa
    15661 tacaaaccaa taagccgagt acagccgtta attttgagcg tagataaaca ctcaatatca
    15721 gcactaaggt tcctattact atacccacaa atagaaacgc tttaggcaac cataaaatag
    15781 gcagatgaaa tagaatcagg gcaatacaaa aacggtctaa agtcataatt cggcttactt
    15841 tttaaagctg agtatgccgt ttgtgcttaa tattttcaga ataaattttc agattttcga
    15901 cacagttcgc aaaaatactt gaaaaaataa aggcagactt tttagcctgc cttataaaga
    15961 agagatatta tgctgatttt tgatcttcag cgtaacttac tgctgcagtg aataatacgt
    16021 ctgttgatga atttaatgcg gtttcaaccg agtcttgaat cacaccaatc acaaaaccca
    16081 ccccaattac ttgagcagca atatcgtttg gaatattaaa taaactacag gctaatggaa
    16141 tcaataataa tgaaccgcct gccacacctg atgcgccaca agcacagatt gatgcaacta
    16201 cgcttaatag tagtgctgtt gcgaaatcag gttgaatacc ttgtgtataa gctgccgcta
    16261 gggttaatac ggttacagta atcgctgcac ctgccatatt aatacttgca cctaatggaa
    16321 tagctacaga agcgatctca tcacgaacgc ccaaacgctt ggctaaattc atattcaccg
    16381 gaatgttggc tgctgaactg cgggtaaaga atgccgttac accactctct cgtaaacagg
    16441 taaatgtaag tgggtatggg ttgcggcgaa ttttccaata cactaataat ggatttagta
    16501 ctaatgccac aaataacatt gcgccaacta agactactaa taaacgtgcg taatcttcaa
    16561 acgcatcaat accattgatg gcaacagttt cagctactaa accaaatacc ccaataggcg
    16621 caaatgcaat cacaactttc acaacaaacg aaactgcgtt agctaaatca tttaagaatg
    16681 cttttgaact tgccgaacca tggcggaatg caatccctaa accgatagac cacgcaagaa
    16741 taccgataaa gtttgcatta cttaatgcga ctaatggatt atcaactaag ttaaaaataa
    16801 cggtttttag aatttcacca atgccttgtg gcggggttaa accgtctggg cttgcagtaa
    16861 gctctaatgt agttgggaac ataaagctta aaacaacagc cgttaatgcc gcaaaaaatg
    16921 tacctaatac ataaagcacc agaatcggct ttaatttgct atctgaacca acttctttat
    16981 ttgcaatcgc agccaaaact aatacaaata ctaaaatagg cgcaatggca cgtagtgatt
    17041 tcacaaaaaa ttgacctaat acaccaacgc tggtagccca ttctttatta ataaatgcca
    17101 gcaatacccc tagcactaaa ccaatggcga tacgtaatac taaattaccg ccaagaattt
    17161 ttgatgata
//
LOCUS       DANLZC010000044        16525 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.044, whole genome shotgun sequence.
ACCESSION   DANLZC010000044 DANLZC010000000
VERSION     DANLZC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16525
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.044"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            212..508
                     /locus_tag="PXE44_001092"
     CDS             212..508
                     /locus_tag="PXE44_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL5588612.1"
                     /translation="MTAITIPQFEEVTSKQFQSEFGRIVDIVKGKTPVAITQYGRPTM
                     VLFPYDQAVEARRLLAKQRLLEMLAERDKNPPAALAEITLEELDKMIDEERNAL"
     gene            498..914
                     /locus_tag="PXE44_001093"
     CDS             498..914
                     /locus_tag="PXE44_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HDL5588613.1"
                     /translation="MHSDYLVIDTNILISGLLFPKSNPNLAVKKALLGYTICVSQATF
                     DEFLEVSARPKFAKYFSIQEREEFIANLLDAVKFVEIKETITDCQDPKDNKFLEVAIS
                     AKAKYLVTGDKKDLLSMNPYRNVEIISVSEFLAETR"
     gene            914..2572
                     /locus_tag="PXE44_001094"
     CDS             914..2572
                     /locus_tag="PXE44_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HDL5588614.1"
                     /translation="MKRFLTLFLLLFSSFSLAETHQIGILAQRGEAYTRTHWQPWVDW
                     LNSQFPNERFELVPLGINEASRRAELDFLLTNQAQFFYLSNQNVRWLATLNSPFSANG
                     EQGAVGSSIWVRADSHYQQLSDLKNQTISAVDNDAFGGFLLGLYQFHQAGMRQNRDFS
                     VQFSGFPVENALALLAEKQVEAAIVPVCLLEELEKEGKFKRSDFRLILQNPQAQGCLA
                     STPLLPNWSLAAMENVPNELAVQLATRLLNSHNPDLPRWTLPFSSAQADHILRELYRH
                     PQQKSLWATVLDWVRLNKFGLLAVVLFILLNLVALRYQVYRKSKALQQAHCKMQQYQQ
                     ELTRADRLALLGEMTTGFAHELKQPLSAVRMYAEGLKSQNSDPYQQRILDKLIAQVDR
                     AVKTMQSIRDWAQNRPSGEPQAVILNQLITNVIEFVAVENRQNAQISLIADRTFRLNL
                     HATVLEQVLTNCLLNALQAGASEISVRLQAVENGLEIAIEDNGGGFSPAQLEFPFVPF
                     RTDKPHGLGLGLVLCQRLMQSLNGRIVLANGENGAKVVLLLPQI"
     gene            2658..3305
                     /locus_tag="PXE44_001095"
     CDS             2658..3305
                     /locus_tag="PXE44_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL5588615.1"
                     /translation="MIIHILDDEESILDAMSFLLAPLGVEIQTWQSSLDFLAQADLHR
                     QGVLLLDIRMPLPDGQQVHQQLREAQSTLAVVIMTAHGDVPMAVAELKKGAVDFLQKP
                     ASFEQLKQAIAQAEKVSEKAVKIREISQNYAKLTEKERNLVPLIMQGFTNKQIADKLA
                     ISVRTVEVHRANVMEKMQAESLAGLVQMIGELQYKGSGNCSATFSFDFCRINLLL"
     gene            complement(3289..4227)
                     /gene="ttcA"
                     /locus_tag="PXE44_001096"
     CDS             complement(3289..4227)
                     /gene="ttcA"
                     /locus_tag="PXE44_001096"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDL5588616.1"
                     /translation="MTDDIQKSDKKITYNFNKLQKRLRRNVGNAIADFGMIEEGDRVM
                     VCLSGGKDSYTLLDILLNLQQSAPVNFSVVAVNLDQKQPGFPEEVLPRYLESIGVEYK
                     IVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLATLF
                     LNMFYGGKMKSMPPKLISDDGKQIVIRPLAYCKEKDIIKYAEAKQFPIIPCNLCGSQP
                     NLQRQVVKEMLNTWDRQYPGRLETMFSALQDIVPSHLCDPKLFDFKGIKHGQTIEGIE
                     GDTAFDDVKIEAKTFLYEDDDNNFAEAGMIQFKEVN"
     gene            complement(4290..5003)
                     /gene="ubiG"
                     /locus_tag="PXE44_001097"
     CDS             complement(4290..5003)
                     /gene="ubiG"
                     /locus_tag="PXE44_001097"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HDL5588617.1"
                     /translation="MQNVDQQEITKFENMAKTWWDPNGSFKPIHLLNPLRLAYILEKS
                     NGLFGKKVLDVGCGGGILSEAMAKQGAIVTGIDMTSQPLEVAKQHAKESGLEIDYQQT
                     TIENFLAKMTACNQEKFDVITCMEMLEHVPDPLSIIQSCKALLKPDGVLFFSTINRTF
                     KAYMLVIIGAEYVLKMLPKGTHEFEKFIKPAELLAWCDQADLRCQEMRGYHFNPLTEK
                     FWLNNDVSCNYIATLKPNN"
     gene            5068..5448
                     /locus_tag="PXE44_001098"
     CDS             5068..5448
                     /locus_tag="PXE44_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDL5588618.1"
                     /translation="MTRINLVPPTELCDQHLLAEHRELTRIPNAVAKGKFSLKGQPED
                     YKLGEGHVRFFFNKLTFLKKRYDALHEECLARGFKVQYFWAEHLPQDPSLWLDYQPTE
                     TALTVNRERIALRMPANPRFSERK"
     gene            5545..8241
                     /gene="gyrA"
                     /locus_tag="PXE44_001099"
     CDS             5545..8241
                     /gene="gyrA"
                     /locus_tag="PXE44_001099"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDL5588619.1"
                     /translation="MSELAKDIIPVSIEDELKTSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFSMDQNSNTYNKPHVKSARVVGDVIGKYHPHGDFAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDAPAAMRYTEVRMQKITQELLTDLDKETVDFSPNYDGKEMIPDVLP
                     TKIPALLVNGSSGIAVGMATNIPPHNLGEVLDGCLAYIENEQISVEELMRYIPGPDFP
                     TGAIINGRKGIEEAYRTGRGKVYVRAKASVETTDKGREQIVVTELPYQVNKAKLIEKI
                     AELVKEKKIEGISKIDDYSDKKGISIVIEVKRDAVGEVVLNHLYSLTQMQVTFGINMV
                     ALDNGQPKVLNLRQIIEAFVKHRREVVTRRTIFELRKARERAHILEGLAIALANIDPV
                     IELIRASKTAEEAREGLLARPWALGNVAPMLEAAGVGASRPEDLPEHLGVRDGEYYLS
                     EAQARAILELRLHRLTGLEHEKIVEEYKELLTEIGELIHILTSPERLMEVIREELERV
                     KTDFNDERRTEITAASGDINIEDLIAQEDVVVTLSHAGYVKYQPLSDYEAQRRGGKGK
                     SATKMKEDDFIEKLLVANTHDTILCFSSRGRLYQLKVYQLPQASRGARGTPIVNILPL
                     VKEENERITAILPIPNGEFSADKFIFMATASGVVKKVSLDAFSNVRASGLIALKLREG
                     DELIGVDITDGNSEIMLFSAQGRVVRFAETAVRAMGRTATGVRGIKLSTTEISSEEIE
                     EAEIIETDNEEAEDSSSVSLDKDRVVSLVIPRTEGDILTVTQNGYGKRTVISEYPVKS
                     RATKGVVSIKVNERNGKVVAAVQVEETDQIMLITDAGTLVRTRVAEVSLVGRNTQGVR
                     IIRTAEDEQVVSLERVCEPEDDENESLDALENAETAETSTEE"
     gene            8336..10300
                     /locus_tag="PXE44_001100"
     CDS             8336..10300
                     /locus_tag="PXE44_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5588620.1"
                     /translation="MALINLSNAYLGFGDHPLLDHTELHIEPGERVCLVGRNGAGKST
                     LMKILAGEVQLDDGKLVFEKDIVVTRLEQDPPRHIEETVFDYVAEGIAHLADLLKQYH
                     HISLQMQTDYSDELLAKLSAVQSQLEHANGWQFENRIQDTLKLLELAPDKRLCELSGG
                     WVRRAALARALVADPDVLLLDEPTNHLDIEAIAWLENLLLDFKGSIIFISHDRAFIRK
                     MATRIVDLDRGKLVSYPSNYDKYLEEKAEDLRVEALQNELFDKKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKALREERRNRREVQGTAKIQIDNTARSGKIVFEVENAGYEIGGRTL
                     LKNFSTTILRGDKIALIGANGIGKTTLIKLLLGEIQPTSGSVRCGTKLEVAYFDQYRV
                     DLDPEKTVMDNVADGKQDVEVNGVKRHVLGYLQDFLFPPKRAMTPVKALSGGERNCLL
                     LAKLLLKPNNLLILDEPTNDLDVETLELLEEMLTDYQGTLIIVSHDRQFVDNTVTECY
                     FFEGEGVVNKYVGGYFDAKQQQENYFAATADKRLNSSKNLQKIEEKAPLAEQEKQKAD
                     QEKTKAEPTKKTKLSYKEQRELDELPAKMEQLEADIEALQAEVNSAEFFAKDPSYTNA
                     QLQKLADTEAALEQAFERWEALENIKNGNI"
     gene            10382..11140
                     /gene="tcdA"
                     /locus_tag="PXE44_001101"
     CDS             10382..11140
                     /gene="tcdA"
                     /locus_tag="PXE44_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDL5588621.1"
                     /translation="MTERLDNYEQRFGGIGRLYTPEGLEKLRRSHICVIGIGGVGSWA
                     VEALARSGIGKITMIDMDDICVTNINRQIHAMSGTVAQLKTEAMKERIERINPECVVE
                     IIDDFLTQDNLADYLNRGYDYVIDAIDSLRVKAAMIAFCKRNKIKIITTGGAGGQTDP
                     TKIQITDLSKTVQDPLAARVRSLLRKEYGFSNKNKFGVECVSSTQQLIFPQMGEACEV
                     SATMNCANGFGAVTMVTATFGFFAVSRVIDKLLK"
     gene            11187..12554
                     /locus_tag="PXE44_001102"
     CDS             11187..12554
                     /locus_tag="PXE44_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HDL5588622.1"
                     /translation="MAIHRVNPKGSMEQLSHLEMELLAKNTQGNLHQLYRNCSLAVLN
                     SGVHTDDSRALLSQYPDFEIRLLTREKGVSLELHNPPETAFVDGKMILNIQYHLFAVL
                     RDIVFVNALKNAIRPLEETSLEALTTNTVFSILRNAKAIEMNTDPNLVVCWGGHSINE
                     TEYQYCRAVGQEFGLRELNIVTGCGAGVMEAPMKGAAIGHANQRYKNGRFIGITEPSI
                     IASEPPNPIVNELVIMPDIEKRLEAFVRLGHAIVVFPGGPGTLEELLYIIGIKLNPAN
                     HAQQLPLILTAPKESADYFYAIDQFIGETLGEEARSLYEIIIDDSVLVARKVKQAMIE
                     VKSSRYESGDAYNFNWSLKIEEDFITPFIPTHENMANLNLSLAQPKEQLAANLRRAFS
                     GIVAGNIKPETSDLIEKYGVFKLHGDRKLMEHIDKLLERFVVQHRMKIPTGEAYQPCY
                     EIVKG"
     gene            12574..14121
                     /locus_tag="PXE44_001103"
     CDS             12574..14121
                     /locus_tag="PXE44_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDL5588623.1"
                     /translation="MYLKHIEISGFRGINHLSLQLRPNMVLIGENAWGKSSLIDALSL
                     IFNLEQALYQFQFKDFHIQYTDTPNQVRDIQLVFTFCEEHQHDLAVQFPAYLAQAAFY
                     IDNHRCVSLAVTGKLHGEQISTDYQFVDQSLKPIQIEQAENAIRQFIYHHPVYRLRDA
                     RLNNRVHNELLLKGSSQLEDDFQRELNALSSLLKYYFLTSQSRYLIKTSMQDTALWWE
                     KVKSLCLRLKQQPELVESAQQHISTLFLFNPRSTVIEKPIILFEDLNAQLHPRMIAIF
                     WELLGFLPSQRITTTNSIELLSQVPLREICRLVRHHDHTQAYWLERHSLGKEDLRKLT
                     FHIHHNRGLALFAKAWILVEGETEVWILQELARLLDIHLEMEGIKIVEFAQCGLRPLI
                     KYARAMGIEWYVLTDGDDSGKRYADTVKSMEEDFAHRLTMLPQKDIEHFFYKAGLRNV
                     FIQLANWRSQEKYPVSYIIKRAIQRSSKPDLAIALANEMGQRGEHSIPTLFKQLFADV
                     LALINKV"
     gene            14344..15378
                     /gene="metN"
                     /locus_tag="PXE44_001104"
     CDS             14344..15378
                     /gene="metN"
                     /locus_tag="PXE44_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDL5588624.1"
                     /translation="MIQLKNISKQFEVKGKKITALDNVNLEVPRGTIYGVIGASGAGK
                     STLIRCVNLLERPTIGQVIVDGQDLTAMSDKELIHARRNIAMIFQHFNLLSSQTVFEN
                     VALSLRLSNTPKEQVEKKVNELLDLVGLTDRKDVYPANLSGGQKQRVAIARALASDPH
                     VLLCDEATSALDPATTQSILQLLKDINKRLGLTILLITHEMEVVKRICDRVAVIDKGQ
                     LIESGSVSEIFSNPKTELAQKFIQSTFHFELPQEYTEQLSSSPTEHSKPIIKFEFTGR
                     SVDAPLLSMASKKFGIDFSILMSQIDYAGGVKFGFVIAEVSGNPDSIAEAKFYLIDNN
                     VKVEVLGYVG"
     gene            15368..16045
                     /locus_tag="PXE44_001105"
     CDS             15368..16045
                     /locus_tag="PXE44_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5588625.1"
                     /translation="MWADFMQEMTPQMWSLVWESTFETVYMGFVATALAVVIGLPIGF
                     LAFLTGKGEILENPRLHQVLDVVINIGRSIPFIILLIILLPFTRLVVGTTLGTTAAIV
                     PLSVSAIPFFARLTSNALLEIPSGLTEAAKSMGATNWQVVTKFYLPESLPILINGITL
                     TLVALIGYSAMAGAVGGGGLGNLAISYGEHRNMVYVKWIATIIIVAIVMISQKLGDHL
                     AKRYDHR"
     gene            16068..>16525
                     /gene="metQ"
                     /locus_tag="PXE44_001106"
     CDS             16068..>16525
                     /gene="metQ"
                     /locus_tag="PXE44_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter membrane-anchored
                     lipoprotein MetQ"
                     /protein_id="HDL5588626.1"
                     /translation="MSFKKILGVALVSALALTACKEEKKAESTAAPAAQAPAKIKVGV
                     MSGPEHTVAERAAQIAKEKYGLEVEFVLFNDYALPNTAVSKGDLDANAFQHKPYLDKD
                     SQSKGLNNLVIVGNTFVYPLAGYSKKVKNVSELAEGAVIAVPNDPSNLARA"
BASE COUNT         4782 a         3347 c         3967 g         4429 t
ORIGIN      
        1 tatggatacc tgaccgcagg cctggagata gaacctacat ataatatgct ctactacatt
       61 aagccataaa aaatagaaaa tcaagtgttt gttcaagttt ttatgttatt gaatttacaa
      121 taactacaaa aatttatctt aattggctcg attgattttt acacaaaaca tagctaacat
      181 atccaaaatc actcacatta ggagcttaaa aatgacagcg attactatcc ctcaatttga
      241 agaggtgact tctaagcaat ttcaatctga gttcggcaga attgttgata tcgtaaaagg
      301 caaaacccct gtggcgatta cccaatatgg cagaccgaca atggtgcttt ttccttacga
      361 tcaagcggtt gaagctcggc gattattagc aaaacagcgt ttgcttgaaa tgcttgcaga
      421 gcgagataag aatccgccgg cagctcttgc agaaatcacg cttgaagagt tggataaaat
      481 gattgatgag gaacgtaatg cactctgatt atttagtgat tgatacgaat attttgatta
      541 gtggtttatt gttcccaaaa tcaaatccca atttagcagt gaaaaaagcc ttattaggct
      601 ataccatttg tgtctcgcaa gcgacttttg acgaattttt agaagtctcc gctagaccga
      661 aatttgctaa atatttcagt attcaggaaa gggaagaatt tattgccaat ttgttggatg
      721 cagtaaagtt tgtggaaatt aaagaaacga ttacagattg ccaagatccc aaagataata
      781 aatttttaga agtggcgatc agtgcaaaag cgaagtattt agtgacgggc gataaaaaag
      841 atctcctcag tatgaaccct tatcggaatg ttgaaattat ttccgtcagc gaatttttag
      901 cggaaactcg ctaatgaaac ggtttctcac tctttttctt ctcctttttt catctttttc
      961 attagccgaa acccaccaaa tcggcattct cgctcagcgt ggggaagcct acacccgcac
     1021 ccattggcag ccttgggtgg attggttaaa ttcgcagttt cccaacgagc ggtttgagct
     1081 ggttccattg gggattaacg aggcgagccg ccgggcggag ttggattttc tgctgaccaa
     1141 tcaagctcag tttttctatt tgagtaacca aaatgtgcga tggcttgcca cgctcaacag
     1201 tccgttttcg gcaaatggtg agcaaggggc ggtgggcagt agcatttggg tgcgtgccga
     1261 cagccattat cagcagttaa gcgatctgaa aaatcagact atcagtgcgg tggataacga
     1321 tgctttcggc ggttttttgc ttgggttgta tcagtttcat caagcaggaa tgcggcaaaa
     1381 tcgggatttt tcggtgcagt ttagcggttt tccggtggaa aatgcgttgg ctttgctggc
     1441 ggaaaaacag gtggaagcgg cgattgtacc ggtctgtttg ctggaagagt tggagaaaga
     1501 gggcaagttc aagcggtcgg attttcgctt aattttgcaa aacccacagg cgcagggttg
     1561 ccttgccagc acgccgttgt tgccgaactg gtcgctggcg gcaatggaaa atgtgccaaa
     1621 cgagctggcg gtacagcttg ccacacgctt gctaaacagc cacaatcccg acctaccccg
     1681 ctggacgttg ccgttttctt ccgcccaagc cgatcacatt ctgcgtgaac tgtatcgcca
     1741 cccgcaacaa aaaagcctgt gggcaacggt gctggattgg gttcgtctca ataaattcgg
     1801 cttgttggcg gtggtgttgt tcattttgct caaccttgtc gccctgcgtt atcaggtcta
     1861 tcgcaaaagc aaggcgttac agcaggcaca ctgcaaaatg cagcagtatc agcaggaact
     1921 gacccgagcc gaccgccttg ctttgttggg cgagatgaca accggttttg ctcacgaact
     1981 gaaacagccg ttgtcggcgg tgcgaatgta cgccgagggg ctgaaaagcc aaaacagcga
     2041 tccttatcaa cagcggattt tggataagct gattgcgcag gtagatcggg cagtgaaaac
     2101 gatgcagagc attcgggact gggcgcaaaa ccgcccgagt ggtgaaccgc aagcggtcat
     2161 tttgaaccaa ttgattacta atgtgatcga gtttgtggcg gtggaaaatc gccagaatgc
     2221 acaaatctcg ctgattgccg accgcacttt tcggcttaat ttacacgcca cggtactgga
     2281 acaagtactg acgaactgcc tgctcaacgc cctgcaagcc ggtgcaagcg agatatcggt
     2341 gcggttacaa gcggtcgaaa acgggctgga aattgcaatc gaagacaacg gcggcggttt
     2401 ttcccctgcc cagcttgaat ttccgtttgt gccatttcgc accgacaaac cacacggctt
     2461 agggctaggc ttggtgctat gccaacggct gatgcaatcg ctcaacggac ggattgtatt
     2521 ggcgaacggg gagaacgggg cgaaggtagt gcttttactc cctcaaatat aaatgtaggg
     2581 gcaggtcctg tgcctgcccg taaatcctat caaattcagg gcgggcacag gacccgcccc
     2641 tacggaaaaa agaaaaaatg attattcaca ttcttgatga cgaagaaagc attttagacg
     2701 ctatgtcgtt cctgcttgcc ccgcttgggg tggaaatcca aacgtggcaa tccagcctag
     2761 attttttggc acaggcggat ttacaccggc aaggcgtgct gttactagat attcgaatgc
     2821 cgctgccaga cgggcaacag gttcatcaac aactgcgaga ggcacaatcg accctggcgg
     2881 tggtgattat gaccgcccac ggtgatgtgc cgatggcagt cgctgaattg aaaaaaggcg
     2941 cagtggattt tctgcaaaag ccggcaagtt ttgaacagct aaaacaggcg attgcacagg
     3001 cggaaaaagt aagtgaaaaa gcggtgaaaa ttcgtgaaat ttcgcaaaat tacgccaaat
     3061 tgacggaaaa agagcgtaac cttgtgccac tgattatgca aggtttcacc aataaacaga
     3121 tcgcagataa attagcgatt tcagtgcgta cggtggaagt acatcgagcc aatgtgatgg
     3181 aaaaaatgca ggcggagagt ttggcggggc tggtgcagat gatcggggag ttacaatata
     3241 aaggtagcgg aaattgctct gctaccttta gtttcgactt ttgccgtatt aatttacttc
     3301 tttaaactga atcattcccg cttcggcaaa attgttatcg tcatcttcgt ataagaaggt
     3361 tttggcttca attttgacat catcaaaagc ggtgtcgcct tctatgcctt caatggtttg
     3421 accgtgttta atccctttaa aatcaaacaa tttcggatcg cataagtggg atggcacgat
     3481 atcttgcaaa gcactgaaca ttgtttctaa acgccccggg tattggcgat cccaagtatt
     3541 cagcatttct ttcaccactt ggcgttgtaa attaggctga gaaccacaca gattacacgg
     3601 aataatcggg aactgcttgg cttcggcata tttgataatg tctttttctt tacaataggc
     3661 gagtgggcgg atcacaattt gcttgccgtc atcagagatc agtttaggtg gcatactttt
     3721 cattttgccg ccgtagaaca tatttaagaa taaagtcgcc agcatatcgt cacggtgatg
     3781 ccctaatgca attttggttg cacccaattc ggtggcggtg cggtataaaa tcccacggcg
     3841 aagtcgtgag caaagtgaac aggtggtttt gccctccgga attttctctt ttacgatgcc
     3901 gtaggtgttt tcttccacaa ttttgtattc cacgccaatg ctttctaaat agcgaggcaa
     3961 tacctcttcc ggaaatcccg gttgcttttg gtctaaattg accgctacca cactaaaatt
     4021 caccggtgca ctttgctgta aattgagcaa aatatctaac agcgtatagc tgtctttacc
     4081 gccggaaagg cagaccatta cacggtcgcc ctcttcaatc ataccaaaat cggcaatggc
     4141 attaccgaca ttacggcgta aacgtttttg taacttgttg aaattataag taattttttt
     4201 atcagatttt tgaatatcgt ctgtcataat agttgtacta ggcttagaaa ttagcggtgt
     4261 atagtaaaga tttcgcacta ttttgcaagt taattattcg gcttcagcgt agcgatatag
     4321 ttacaactca catcattatt gagccaaaat ttttcggtta atgggttgaa atggtagcct
     4381 ctcatctctt ggcaacgtaa atcagcctga tcgcaccaag ctaacaattc ggcaggcttg
     4441 atgaattttt caaattcgtg cgtgcctttt ggcaacattt tcagcacata ttccgccccg
     4501 ataattacca acatataggc tttaaaagta cggttgatgg tagagaaaaa cagcacgccg
     4561 tcaggtttga gcagggcttt acagctttga atgatagaaa gcggatcggg aacgtgttcc
     4621 agcatttcca tacaagtaat cacatcgaat ttttcttgat tacaagcggt catttttgct
     4681 aaaaaatttt caatcgtggt ttgttggtaa tcaatctcta acccactttc tttggcgtgc
     4741 tgctttgcca cttcaagcgg ttgagaggtc atatcgatgc cggtaacaat cgctccctgt
     4801 tttgccattg cctcacttaa aataccgcca ccacagccga catctaacac ctttttgcca
     4861 aataaaccgt tcgatttttc taaaatatag gctaaacgca gtggattaag cagatgaatc
     4921 ggcttgaaac tgccgtttgg atcccaccag gttttagcca tattttcaaa ttttgtgatt
     4981 tcttgctgat cgacgttttg cataaactgt tcctatggta aatttagggc attactataa
     5041 cgcatttagc ctaaaaggaa aatcagaatg acccgaatta accttgttcc gccaacggaa
     5101 ttatgcgatc aacatctgct tgccgaacat cgtgaactta ctcggattcc gaatgcggta
     5161 gcgaaaggca aatttagcct aaaaggacag ccagaagatt acaaactggg cgagggacac
     5221 gttcggtttt tcttcaataa actcacgttt ttgaaaaagc gttacgatgc gttacacgag
     5281 gaatgtttag caagaggttt taaggtgcaa tatttctggg cggaacattt accacaagat
     5341 ccctctctct ggctagatta tcagccgact gaaaccgctc tgactgtaaa tcgggagcga
     5401 attgcattaa gaatgccggc aaatccacgt tttagcgaaa gaaaatgacc gcttgtacgc
     5461 ctttttgttg ctttttatgc tataatctcg ccaatttttt cgccttattt aaggctcttt
     5521 tactaataaa attaggaaat ttcaatgagc gaattagcca aagatattat tcccgtaagt
     5581 attgaggacg aattaaaaac ctcttacctt gactatgcga tgtcggtgat tgttgggcgt
     5641 gccttgcctg atgtacgaga cggtttaaag ccggtacacc gccgagtgct tttttcaatg
     5701 gatcaaaaca gcaacaccta taataagccg cacgttaaat ctgcccgtgt ggtcggtgat
     5761 gtgatcggta aatatcaccc acacggtgac tttgcggtgt atgacactat tgtgcgtatg
     5821 gctcaaccgt tctcactacg ttatatgttg gtggacgggc aaggtaactt tggttctatt
     5881 gatggcgatg caccggcagc aatgcgttat accgaagtgc gtatgcagaa aattacgcag
     5941 gaattattaa ccgatctcga taaagaaacg gtggatttct caccgaacta tgacggcaaa
     6001 gagatgattc ctgatgtatt gccaaccaaa attccggctc tgttagtcaa tggttcttca
     6061 gggattgcgg tcggtatggc aaccaatatt ccacctcata atttaggcga agtattagat
     6121 ggttgcttgg cgtatattga aaatgaacag attagtgttg aagagctaat gcgttatatc
     6181 ccggggccgg atttcccgac cggggcgatt atcaacggcc gtaaagggat tgaagaggct
     6241 taccgcaccg gtcgtggcaa ggtgtatgtg cgcgcgaaag ccagcgtgga aacaactgat
     6301 aaagggcgtg agcaaattgt tgtaaccgaa ctgccatacc aagtgaataa ggcaaaatta
     6361 attgaaaaaa ttgccgagct ggttaaagag aagaaaatcg aaggcatcag caaaattgat
     6421 gactattccg ataaaaaagg gatttcgatt gttattgaag tcaagcgtga tgcagtgggt
     6481 gaagtggtgt taaaccacct ttattcttta acccaaatgc aggtcacgtt tggtatcaat
     6541 atggtggcgt tagataatgg gcagccaaaa gtgctgaact tacgccagat catcgaagcc
     6601 tttgttaaac accgtcgtga agtggtcaca cgccgtacga tttttgaatt gcgtaaagcc
     6661 cgtgaacgtg cccatatttt agaaggtttg gcgattgcgt tagcgaatat cgacccggta
     6721 attgaattaa ttcgagcctc aaaaaccgca gaagaagccc gtgaaggctt attggctcgc
     6781 ccttgggcat taggcaatgt tgcaccaatg ttagaggctg ctggtgttgg tgcttcccga
     6841 ccggaagatt tacctgaaca cttaggagtg cgtgatggcg aatactattt atctgaagct
     6901 caagccagag cgattttaga gctacgctta caccgcttaa ccggtttaga acacgagaaa
     6961 atcgttgaag aatacaaaga actcttaact gaaattggcg aattaatcca cattttaacc
     7021 agcccagaac gcttaatgga agtgatccgt gaagagttag aacgtgtaaa aaccgatttt
     7081 aatgatgaac gccgtactga aattaccgca gcctctggcg acatcaacat tgaagactta
     7141 atcgcacaag aagatgtggt ggttacgctt tctcacgccg gctatgtgaa atatcagccg
     7201 ttatccgact acgaagcaca acgccgtggc ggtaaaggta agtcggcaac caagatgaaa
     7261 gaagatgatt tcatcgaaaa actcttggtg gcaaacaccc acgacaccat tctttgcttc
     7321 tcaagccgtg ggcgtttata tcaattaaaa gtgtatcaat taccgcaagc aagccgtggg
     7381 gcaagaggca cgccgattgt gaatattctg ccgttagtga aagaagaaaa cgaacgtatt
     7441 accgcgattc taccgattcc aaacggtgaa tttagtgccg ataagtttat ctttatggca
     7501 acagctagcg gtgtagtgaa aaaagtttcc cttgatgcct ttagcaacgt gcgtgcaagc
     7561 ggtctgattg cgttaaaact tcgtgaaggc gatgagctga tcggggtaga tattactgat
     7621 ggtaacagcg aaattatgtt gttctcggca caagggcgag tagttcgctt tgccgaaaca
     7681 gcagttcgtg cgatgggacg taccgccacc ggcgtacgag gtattaaact ttctaccacc
     7741 gaaatttcaa gcgaagaaat tgaagaagct gaaattattg aaactgacaa tgaagaggca
     7801 gaagattcct ccagcgtcag ccttgataaa gaccgtgtgg tttctttagt gattcctcgc
     7861 accgaaggcg atattttaac cgtcacccaa aacggctacg gtaaacgtac ggtgattagc
     7921 gaatatccgg tgaaatcccg agcaaccaaa ggggtcgttt cgattaaagt gaatgaacga
     7981 aacggcaaag tggttgcggc agttcaagta gaggaaaccg accaaattat gttgattacc
     8041 gatgccggca cgctggttcg cacccgagtg gcagaagtca gcctcgttgg acgaaacacc
     8101 caaggcgtac gtattattcg caccgcagaa gacgaacaag ttgttagcct tgaacgagtc
     8161 tgcgagcctg aagacgatga aaatgaaagc ctagacgcat tagaaaatgc cgaaacagca
     8221 gaaacatcaa ctgaagaata atcttaacta aacaagcggt cgaattttca caattttttg
     8281 caaaaataga aggaaattcg accgtttttg gatcatacag tagataataa aaactatggc
     8341 acttatcaat ctttccaacg cctatttagg ctttggcgat catcctttat tggatcatac
     8401 tgaattacat atcgaacccg gcgagcgggt ttgtttggtg gggcgtaacg gggcgggtaa
     8461 atcgaccctg atgaaaattc tggcgggcga agttcagctt gatgacggca agttggtatt
     8521 tgaaaaagac attgtcgtta cacgtcttga acaagatccg ccccgccata ttgaagaaac
     8581 ggtgttcgat tatgtggcgg aggggattgc ccatcttgcc gacttattga agcaatatca
     8641 ccacatttcc ctgcaaatgc agaccgatta cagcgatgaa ctgctcgcca aactttcagc
     8701 ggtgcaatcg caattagaac acgctaacgg ttggcagttt gaaaaccgca ttcaagacac
     8761 cttgaaactg ctggaactcg cccccgacaa acgcctgtgc gaactctcag gcggttgggt
     8821 acgccgtgcg gcattggctc gggcgttggt tgccgatcct gatgtattgc tgttggacga
     8881 accgaccaac cacctcgaca ttgaggcgat tgcgtggctg gaaaatctgt tattggattt
     8941 caaaggcagt attattttta tctcgcacga ccgtgccttt atccgcaaaa tggcgactcg
     9001 gattgtggat ttagatcgag gcaaattggt ttcctaccca agcaattacg ataaatactt
     9061 ggaagaaaaa gccgaggatc tgcgagtaga agcgttgcaa aatgagctgt tcgacaaaaa
     9121 actcgcccaa gaagaagtct ggatccgtca agggattaag gcaagacgca cccgaaacga
     9181 agggcgtgtg cgagcgttga aagcgttgcg tgaagagcgt agaaaccgcc gagaggtaca
     9241 aggcacggcg aaaattcaga tcgacaacac cgcacgcagc ggcaaaattg tgtttgaagt
     9301 ggaaaatgcc ggctatgaaa tcggcggcag aaccctgctg aaaaatttca gcaccaccat
     9361 tttgcgtggc gataaaattg ccctgattgg agcgaacggg atcggcaaaa ccacgctgat
     9421 caagctgtta cttggcgaaa ttcagccgac atctggttca gtgcgttgtg gcacaaaatt
     9481 ggaagtggcg tacttcgacc aataccgagt cgatcttgac cctgaaaaaa ccgtgatgga
     9541 caacgtggca gacggcaagc aagatgtaga agtaaacggg gtgaaacgcc acgttcttgg
     9601 ctatctgcaa gacttcctgt tcccgccaaa acgggcgatg acaccggtga aagccctctc
     9661 gggtggtgaa cgcaactgct tgttgctggc aaaactgtta ctcaaaccga acaacttgct
     9721 gattttggac gaaccgacca acgatttgga cgtggaaacc ctcgaactgc tggaagagat
     9781 gctcaccgac tatcaaggca ccctgattat tgtgagccac gaccgtcaat ttgtggataa
     9841 taccgtaacc gaatgctact tcttcgaggg cgaaggcgtg gtgaataaat atgtcggcgg
     9901 ctatttcgat gccaaacaac agcaggaaaa ttacttcgcc gccactgccg acaagcggtt
     9961 aaattcgagt aaaaatttgc aaaaaataga ggaaaaagca ccgcttgcgg agcaagagaa
    10021 acagaaagcc gatcaggaaa aaacgaaagc agagccgacg aagaaaacca aactttccta
    10081 caaagaacag cgtgaactgg acgagttacc ggctaaaatg gaacagcttg aagccgacat
    10141 tgaagccctg caagccgaag taaatagtgc cgaattcttc gccaaagatc caagctacac
    10201 caacgcccag ctacaaaaac ttgccgatac cgaagccgcg ttagagcaag cctttgagcg
    10261 gtgggaagcg ttggaaaata ttaaaaacgg gaatatctaa tgtaggggcg aatgattatt
    10321 cgcccttgat ggataaaatg attgggcgaa aaatttttcg cccctacaaa tgtgaagcaa
    10381 aatgacagaa agattagata actacgaaca acgttttggt ggcattggtc gcctttatac
    10441 gccggaagga ttggagaaac tacgccgttc gcatatttgc gtgattggga ttggcggggt
    10501 tggctcgtgg gcagtggagg cattggctcg ctcaggtatc ggtaagatca cgatgatcga
    10561 tatggacgat atttgcgtga ccaatatcaa ccgccaaatt cacgcaatga gtggcacggt
    10621 ggctcaactg aaaaccgaag cgatgaaaga gcggattgag cggatcaacc ctgaatgtgt
    10681 ggtggaaatt attgatgatt tcttaacgca ggataatctc gcagactacc tcaatcgagg
    10741 atatgactat gtgatcgatg cgattgacag tttgcgggta aaagcggcga tgattgcgtt
    10801 ttgtaaacgc aataagatca aaattatcac caccggcggg gcgggcgggc agaccgatcc
    10861 gaccaaaatt caaattaccg atttaagcaa aacggtgcaa gatccgttgg cggcaagggt
    10921 tcgtagcctg ttgcgtaaag agtacggttt cagcaataaa aacaaattcg gcgtggagtg
    10981 cgtttcttcg actcagcaac tgattttccc acaaatgggt gaggcctgtg aggtgtcggc
    11041 aacaatgaac tgtgccaacg gctttggagc ggtgacaatg gtgacggcaa cgttcggctt
    11101 ttttgcggtg agtcgggtga ttgataaact attaaaataa caagcggtca tattaaatga
    11161 attttttgca aatcaaggag taaataatgg caatacatcg agtcaacccg aaagggagta
    11221 tggaacagct ttcccatttg gaaatggagt tgttggcaaa gaatacgcag ggtaatttgc
    11281 atcaacttta tcgtaattgc tctttggcgg tgctaaattc cggtgtgcat accgatgaca
    11341 gccgagcgtt gttaagccaa taccctgatt ttgaaatccg cttattaacc cgtgaaaaag
    11401 gcgtgtcgct tgaattgcat aatccacccg aaactgcgtt tgttgacggc aagatgattt
    11461 tgaatattca atatcacctt tttgcggtgc tgcgggatat tgtgtttgtg aatgcgttaa
    11521 aaaatgcgat tagaccgctt gaagagactt ctttagaagc cttaaccacg aataccgtat
    11581 tttcgatttt gcggaatgcg aaagcgattg aaatgaatac cgacccaaat ttggtggtgt
    11641 gctggggagg gcattcgatt aatgaaaccg aataccaata ttgccgtgcc gtcgggcaag
    11701 agtttggctt gcgtgagctg aatattgtga ccggttgcgg agccggtgtg atggaagccc
    11761 cgatgaaagg agcggctatc ggacacgcca atcaacgtta taaaaacggg cgttttatcg
    11821 gcattaccga gccgtctatt attgcctccg agccgccaaa cccgattgtg aatgaattgg
    11881 taattatgcc ggatattgaa aaacggttgg aagcctttgt gcgtttaggg cacgccattg
    11941 tggttttccc gggcgggccg ggaacgctgg aagagttgct gtatattatc ggtattaagt
    12001 taaacccggc taaccatgct cagcaattac cactgatttt aaccgccccg aaagaatcgg
    12061 cagactattt ctatgccatc gatcaattta ttggcgaaac tttaggcgaa gaagctcgca
    12121 gtttgtatga aattattatt gatgactcgg tgttggtggc tcgtaaagtc aagcaagcga
    12181 tgattgaggt aaaaagcagc cgttatgaaa gtggcgatgc ttataatttc aactggtcgt
    12241 taaaaattga ggaagatttt attacgccat ttattccaac ccacgaaaat atggcaaacc
    12301 tgaatttaag tttagcacag ccaaaagagc agctggctgc gaatttaaga cgggcatttt
    12361 cagggattgt tgcaggtaat attaagccgg agacctccga tttaattgag aaatatgggg
    12421 tgtttaaatt acacggcgat cgtaaattaa tggagcatat agataaactg ttggagcgtt
    12481 ttgtggttca acaccgtatg aaaattccaa cgggtgaggc gtaccaacct tgctatgaaa
    12541 ttgtaaaagg gtaatgctaa caaggggtaa ggtatgtatt taaagcatat tgaaatcagt
    12601 ggctttcgtg gtattaacca tttatcgctc cagctacgac caaatatggt attgattggc
    12661 gaaaatgcgt gggggaaatc cagtctgatt gatgcattaa gcctgatttt taaccttgag
    12721 caggcgttgt atcaatttca gttcaaagat ttccatattc aatacaccga tacccccaac
    12781 caagtgcggg acattcaact ggtgtttacc ttttgtgaag agcatcagca cgatttagcg
    12841 gtgcaatttc ccgcttattt ggcacaagcc gcgttttata ttgataatca ccgctgtgtt
    12901 tcgcttgccg taaccggaaa attacacgga gagcagatta gcaccgacta tcaatttgtg
    12961 gatcaaagcc taaaaccgat ccagatagaa caagcggaaa acgccattcg gcagtttatt
    13021 taccatcacc ctgtttatcg cctgcgtgat gcaaggctca ataacagggt gcataatgaa
    13081 ttattactta aaggatcgtc tcaacttgaa gacgattttc aacgtgaatt aaacgccctc
    13141 tcttcgctac tgaaatacta ttttctaacc agccaaagtc gctatttgat caaaaccagt
    13201 atgcaagaca ccgcattgtg gtgggaaaaa gtaaaatccc tctgtttacg cctaaaacag
    13261 cagccggaat tggtcgaatc tgctcagcag catatttcta ctctattttt gtttaatcct
    13321 agatctacag taatagaaaa gccgattatt ttatttgaag atctgaatgc tcaactacac
    13381 cctcgaatga ttgcgatttt ttgggaactg ctcggttttt tacccagcca acggatcacc
    13441 actactaatt ctattgaatt gttatcgcaa gtgcctctgc gtgaaatctg ccgtttggtg
    13501 cgtcatcacg atcataccca agcctattgg cttgaacgcc acagtttagg caaggaagat
    13561 ttgcgaaaat taacctttca tattcaccat aatcgtggct tggcactgtt tgctaaagcg
    13621 tggattttgg ttgaggggga aacggaggtt tggattttgc aagagctggc gagattatta
    13681 gatattcatt tggaaatgga agggatcaaa atcgttgaat ttgctcagtg tggcttacgt
    13741 ccgttaatta aatacgccag agcaatgggg atcgaatggt atgtgctgac tgacggtgac
    13801 gattccggca aacgttatgc agatacggta aaaagtatgg aagaggattt tgctcaccgg
    13861 ctcacgatgt tgccacaaaa agatatcgaa cattttttct acaaagcagg cttgcggaat
    13921 gtgtttatac agcttgcaaa ttggcgttca caagaaaaat atccggtgtc ctacatcatt
    13981 aaacgagcga ttcaacgaag ctcaaagcct gatttagcca ttgcattagc caatgagatg
    14041 gggcaacggg gagagcattc aattccaact ttatttaaac aattatttgc agatgtgctg
    14101 gcattgataa acaaagtata gaatacaagc ggttaaattt gacaaaaaat tgccaatttt
    14161 gaccgcttgt ttgcttgtga tgaaaaccaa tcatacaaaa ctctaattta tttcaagtgc
    14221 aagctattat tttatgcgtt atgcttattt ggaataaaaa aatcgggtta gttgattgac
    14281 atagcattct agacgtctaa aataaccaaa aataatagtt aattgaatgt ggataaatag
    14341 aaaatgattc agctgaaaaa tatcagcaaa caatttgagg taaagggcaa aaaaattacc
    14401 gctctggata atgtaaacct agaagtacca agaggcacta tttatggtgt cattggggca
    14461 tcaggggcag gaaaaagtac cttaattcgc tgtgtaaatt tattagaacg cccaacaatc
    14521 gggcaagtga ttgtcgatgg gcaagattta acggcaatgt cggataaaga gttgattcac
    14581 gctcgccgta atattgcaat gattttccaa cattttaatt tgctttcatc acaaactgta
    14641 tttgaaaacg tggcgttatc gttgcgttta agcaatacgc caaaagagca agttgagaag
    14701 aaagtcaatg agctgttgga tttagtgggg ttaacagatc gtaaagatgt ttaccctgca
    14761 aatctttccg gtgggcagaa acagcgtgta gcgattgctc gtgcgttagc cagcgatcca
    14821 cacgttttgt tgtgtgatga agcgaccagt gcgttagatc cggcgactac gcaatccatt
    14881 ttgcagctat taaaagatat taataaacgt cttggtttga cgattttatt aatcactcac
    14941 gaaatggaag tggtaaaacg catttgtgac cgagtagcag tgattgataa agggcagttg
    15001 attgaatctg gctcggtaag tgaaattttc tcgaatccga aaaccgaatt agctcagaaa
    15061 tttattcaat cgactttcca ttttgaatta ccgcaagaat ataccgagca gctttcaagt
    15121 tcgccgactg aacatagtaa gccgattatt aaatttgagt ttaccggacg ttcggtggat
    15181 gcacctttgc tttcaatggc atcgaagaaa ttcggcattg attttagtat tttgatgtcg
    15241 caaattgatt atgcaggcgg ggtcaaattt ggatttgtaa ttgcagaagt gagtggtaat
    15301 ccggattcca ttgcagaggc aaaattttat ttaattgata acaatgtaaa agtagaggtg
    15361 ttaggctatg tgggctgatt ttatgcagga gatgacgcca cagatgtgga gcttagtatg
    15421 ggaatctact tttgaaaccg tttatatggg ctttgtggca acggcattgg cggtagtcat
    15481 cggtttaccg attggtttct tggcgttttt aaccggtaaa ggcgaaattt tagaaaatcc
    15541 gagattacac caagtgttag atgtcgttat taacatcggg cgttcgattc cgttcattat
    15601 tttattgatt attttgttgc catttactcg cttagttgtg ggcacaacat taggcacaac
    15661 ggcagcgatt gttcctttaa gtgtttcggc cattccgttt tttgcccgtt taacttcaaa
    15721 tgcgttgctt gaaattccgt ccggtttaac agaagcggca aaatcaatgg gagcaacaaa
    15781 ttggcaggta gtgacaaaat tttatttacc ggagtcatta ccgattttga ttaatggcat
    15841 tacgctaacg ctggttgctt taattggcta ctcagcaatg gcgggggctg ttggtggcgg
    15901 cggtttaggc aatttagcga ttagctatgg cgaacaccgt aatatggtgt atgtgaaatg
    15961 gattgctacc attattattg ttgcgattgt gatgattagc caaaaattgg gtgatcattt
    16021 agcaaaacgt tacgatcatc gttaatttta tttaaaaaga ggtttctatg agtttcaaga
    16081 aaattttagg cgttgcattg gtttctgcat tagcattaac cgcttgtaaa gaagagaaaa
    16141 aggcagaatc taccgctgct ccggcagctc aagctccggc taaaatcaaa gtaggggtaa
    16201 tgtcaggccc tgagcatacc gttgcagaac gtgcagcaca gattgcaaaa gaaaaatatg
    16261 gtttagaagt tgagttcgtt ttatttaacg actacgcttt accgaatact gcagtaagta
    16321 aaggtgattt agatgcaaac gcattccaac acaaaccata tttagataaa gacagccaat
    16381 caaaaggttt aaacaactta gtgattgtgg gtaacacctt tgtttatccg ttagcgggct
    16441 actctaaaaa agtgaaaaat gtgtctgagt tagcagaagg tgcggtaatt gcagtaccaa
    16501 atgatccttc aaacttagct cgtgc
//
LOCUS       DANLZC010000045        16113 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.045, whole genome shotgun sequence.
ACCESSION   DANLZC010000045 DANLZC010000000
VERSION     DANLZC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16113
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.045"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            185..2407
                     /gene="glgB"
                     /locus_tag="PXE44_001107"
     CDS             185..2407
                     /gene="glgB"
                     /locus_tag="PXE44_001107"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HDL5588627.1"
                     /translation="MQDYTYSEKDAFFVERLSSGSCNDPFSYLGIHPSNNGVIIRVFL
                     PSAIACQVIDLNGKPVSSMLKIDDRGFFIAELIGKKTDFSYRLLVEYPDSTVDIEDPY
                     RFKTHIQELDNWLFAEGKQLRPYEKLGAHLVTQNEVGGVHFSVWAPNAQRVSVVGDFN
                     HWNGLVHSMRFHPDSGIWDIFLPNVKKGECYKFEVLDRNGVLRLKSDPYAFASQFRPE
                     TASIVSGLPSIVPAEKTCRNANAPDKPISIYEVHLGSWRRNIENNYWLSYEELADELI
                     PYVKDMGFTHIELLPISEFPFDGSWGYQTTGIYSPTSRFGTPEGLSYFIQKAHDKGIG
                     VILDWVVGHFPVDEHGLVYFDGTHLYEHSDPKEGYHKDWNTLIFSYGRNEVSNYLSGN
                     ALYWIERFGIDALRVDAVASMIYRDYSRSDGEWIPNKYGGRENLEAIDFLRHTNNILR
                     DESQGGASIAEESTSFAGVSHSTERGGLGFNYKWNMGWMNDTLRYMQKDPIYRRYHHN
                     LMTFGMTYQYSEQFVLPLSHDEVVHGKGSLLNKMPGDCWQKFANLRAYYGYMWGYPGK
                     KLLFMGNEFAQGKEWNFAESLDWYLLGEEGGEWHKGVLGWVKALNHLYTQTPALFEQD
                     YNPEGFEWLVVDDHDNSVFAFERKAKNGDSVIVVSNFTPIVRENYRFGVNELAEYQEI
                     LNSDLACYCGSNVSNANRFASELIASHGKAYSLSITLPPLSTIFLKRAADKKTKQNKT
                     "
     gene            2431..4443
                     /gene="glgX"
                     /locus_tag="PXE44_001108"
     CDS             2431..4443
                     /gene="glgX"
                     /locus_tag="PXE44_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDL5588628.1"
                     /translation="MHYFRGKPFPLGNKLDRQAGEFGTNFAIVSQNATAIELCLFDEN
                     HHETRIPMFSSGDTWHIFVAGVQAGTKYGYRVKGIQDEQAGLLFNPQKLLTDPYAKAI
                     EGKPDLSSAEAAAWYKWNDERDNASLAPKSIVIDEHFDWENDSQLFTPWAETIIYEMQ
                     VKGFSQRNFQIPSDIRGTFAGLAHPVSIDYLKTLGITAVELLPVTYHIDETHLQKQGL
                     VNYWGYNVLGNFAIDPNLAADKQNPLKEFKQLVKTLHQAHIEVIMDIVFNHTAESDKN
                     GPMLSFRGIDNTAYWLDKQGEYENWTGCGNTLNLSNPYILCWAIDCLIYWVEECHIDG
                     FRFDLASILGRTPSFSEKADFFTALKSHSKLTHIKLIAEPWDIGVEGYQLGQFPEPFY
                     QWNDCYRDDIRRFWLQNQGDWGLLARRLAGSDDVFTKYSVSKSINFITAHDGFTLQDL
                     TSYQQKHNWANGEQNRDGHNSNYSNNHGVEGQTADELVKKQREITACSLLATLFLSAG
                     VPMLLAGDEIGHSQQGNNNSYCQDNEITWLDWENMNHTRLDYVRSLIRLRKKIALLSK
                     QNNWWQEDSVQWLNPSAQLMNIDDWHNQHNGLVVLLQEEWLLLFNANRQPQPFLLPQG
                     NWKVVLEQAQGILMTDTQAVTLNNMGVCVLQKSDVMFANKLETQYA"
     gene            4436..5749
                     /gene="glgC"
                     /locus_tag="PXE44_001109"
     CDS             4436..5749
                     /gene="glgC"
                     /locus_tag="PXE44_001109"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDL5588629.1"
                     /translation="MLNQESTALVARQDLTNDTLVLILAGGRGSRLYELTDKRAKPAV
                     YFGGSRRIIDFALSNCINSNLLKIGVITQYAAHSLLRHLQRGWSFLPYERNQYIDMLP
                     ARQQLDENTWYRGTADAVYQNMAMMKSHYRPKYVVILAGDHIYKMDYTQMLKDHVDQG
                     AKCTVGCIEVPRSQATEFGVMAVNEKLKVKEFVEKPADPPAMPGKPDSSLASMGIYVF
                     NADYLYEMLEKEASTPGTSHDFGKDIIPKAVEQGVLYAHPFERSCKGRNASGAIYWRD
                     VGTIDSYWAAHMDLVSEDPELDLYDESWPIHGRPQQTAPARFFYNRARARTLDNSLIA
                     GGCVITDAEISNSVLFNRVHVEAESVIEHTVILPQVTIGKNCVIKRAVIDRHCVIPDG
                     LEIGVNAEEDAKRFRVSKNGIVLVTQSMLDKLAGKEANSDIKYTI"
     gene            5872..7308
                     /gene="glgA"
                     /locus_tag="PXE44_001110"
     CDS             5872..7308
                     /gene="glgA"
                     /locus_tag="PXE44_001110"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDL5588630.1"
                     /translation="MKVLHICSEMYPLIKTGGLADVMGALPFAQQQAGNDVRVLIPYY
                     PKVAQTLGETVEVATIGTFAGTVSVRFAYFNGLGVYVIDAPHLFMRDGNPYHDSNYQD
                     YPDNYKRFALLGYIGAQLAQGLDHWWGTAQVLHAHDWQAGLACAYLKSWGSNVKTLFT
                     IHNIAYIGRFNAFHLAELGLPWEFFHAEGLEFYGEISYLKAGLYFADRITTVSPTYAR
                     EITEEIAGAGLHGLLATRQYQGRLRGILNGVDAEVWNPETDTHIVVNYRANYMHGKAK
                     NKAELQRIFGLPEDKEALLMVMVTRLTEQKGADFLLANIDTLMQHKVQIIVLGSGSPD
                     LENALRYFQHKYPNQIGVKIGYDEALSHQIIAGGDLILVPSRFEPCGLTQLYGLKYGT
                     LPLVRRTGGLADTVVDSHKESIENRSATGFVFDYPDAPAFLEAAMRAISLWQKQKLWS
                     SVRQNALAQDFGWAKVAEQYQNLYHEII"
     gene            7403..9903
                     /locus_tag="PXE44_001111"
                     /pseudo
     CDS             7403..9903
                     /locus_tag="PXE44_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
     gene            complement(9990..10706)
                     /gene="trmJ"
                     /locus_tag="PXE44_001112"
     CDS             complement(9990..10706)
                     /gene="trmJ"
                     /locus_tag="PXE44_001112"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDL5588631.1"
                     /translation="MQILNQIQIILVETSLPANIGSAARAMKTMGLTHLRLVAPKQPL
                     DEQAEALAAGAKDVLDNAQCYATFDEAIADCQLVIGTSARLRHLQNTLIEPRDCGELV
                     VNRTNLGKVAIVFGRERVGLTNEELLKCQYHLNFPTNPDYGSLNLAMAVQLASYEIRM
                     AYLQNSGEKQPLAADKDYPTAQALEHFFAHTERLYTELGFIRNDSVMLKLRRLYQRAE
                     LETNELNLLRGMLTAVEKAN"
     gene            10886..12424
                     /gene="pntA"
                     /locus_tag="PXE44_001113"
     CDS             10886..12424
                     /gene="pntA"
                     /locus_tag="PXE44_001113"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDL5588632.1"
                     /translation="MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEQDAGFKAS
                     FEDNTFLNAGANIGTQQEVWNADIIFKVNAPTEAEIELIKEGVTLVSFIWPAQNPQLM
                     EKLQSKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAA
                     GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEE
                     EGGSGDGYAKVMSEEFNRRAMALYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMK
                     PGSVIVDLAAATGGNCEYTKAGEIVVTDNQVKVIGYTDFPARLPTQSSQLYGTNLVNL
                     LKLLAPNKDGQIEINFDDVVLRGVTVVRDGELTWPAPPIQVSAQLQKQAAAPQAVKKE
                     EKPADPRVKYGVMAAAGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHT
                     PLMAVTNAISGIIIVGAVLQIAQPTGNFFIDILAFIAILVASINIFGGFKVTQRMLAM
                     FRKG"
     gene            12439..13884
                     /gene="pntB"
                     /locus_tag="PXE44_001114"
     CDS             12439..13884
                     /gene="pntB"
                     /locus_tag="PXE44_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDL5588633.1"
                     /translation="MSFGFVTAAYIIAAILFIMSLAGLSKHETAKAGCWYGIVGMAIA
                     LVATIFGPQSHGTIWILIAMAIGGFIGVKKALKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYGLHTNALMPANLDETAQAAFLAEQATLANIHNVEVFLGIFIGAVTFSGSLVAFG
                     KLSGKLFGRKVSSAALNIPHKHKWNLAAIIVSVFLMIVFLNNPHNIFPVLIMTIIALV
                     FGWHLVSSIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYI
                     MCKAMNRSFISVIAGGFGNEVQSGKGDEEYGEHRETKAEEVAEMLKNASSVIITPGYG
                     MAVAQAQYPVAEITAKLREKGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDVVLEMDE
                     INDDFEETDVVLVIGANDTVNPAALDDPSSPIAGMPVLEVWKAQNVIVFKRSMAVGYA
                     GVQNPLFFKENTQMLFGDAKERVDDILRALN"
     gene            complement(13951..14877)
                     /locus_tag="PXE44_001115"
     CDS             complement(13951..14877)
                     /locus_tag="PXE44_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5588634.1"
                     /translation="MKPIFLEIRHLRTLIALKESGSVSLAAKRVHLTQSALSHQIKLL
                     EEQYDLTLFERKSSPIRFTPAGERLIKLAYDILPKVIEAELDLARVKQGEVGEVRVAV
                     ECHTCFDWLMPAMDTFRQHWPLVELDIVSGFHTDTVGLLLSHRADLAIVAEEEDSAGI
                     IYKPLFSYEMVAICSKDHPLATKDVWEAEDFKDETLITYPVPDDMLDLLRKILGPAGI
                     KVTRRNSELTIAIIQLVASRRGIAALPYWAVKPYLDRGYVVARKITQQGLYSTLYGAI
                     READATLAYLDDFHDTVKSQSFATLPGLMVLE"
     gene            complement(14987..16102)
                     /gene="asd"
                     /locus_tag="PXE44_001116"
     CDS             complement(14987..16102)
                     /gene="asd"
                     /locus_tag="PXE44_001116"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL5588635.1"
                     /translation="MQNVGFIGWRGMVGSVLMDRMVEENNFANINPVFFTTSQAGQKA
                     PVFGGKDAGELKNAFDIEELKKLDIIVTCQGGDYTNEVYPKLKETGWNGYWIDAASAL
                     RMKDDAIIVLDPVNQHVISEGLKNGIKTFVGGNCTVSLMLMAIGGLFEKDLVEWVSVA
                     TYQAASGAGAKNMRELLSQMGELEDSVKAELANPASSILDIERKVTAKMRAEDFPTEN
                     FGAALGGSLIPWIDKLLPETGQTKEEWKGYAETNKILGLSDNPIPVDGLCVRIGALRC
                     HSQAFTIKLKKDIPLAEIEQIIAAHNEWVKVIPNDKETTLRELTPAKVTGTLSVPVGR
                     LRKLAMGGEYLAAFTVGDQLLWGAAEPVRRILVQLTQ"
BASE COUNT         4620 a         3114 c         3681 g         4698 t
ORIGIN      
        1 atgtttcaaa atgtgatcta tgtcacaaaa tttaaggttt gatgatttaa actaaagatt
       61 aaaacgattt tattttgtga tttagctcaa aaaatggcgt aaatccactt cttttatttc
      121 tatttagtga tagagtgaga actattgaaa tttaagtatt aaacgcttta agcaaggagt
      181 gccgatgcaa gactatactt attctgaaaa agatgctttt tttgttgagc gactttcttc
      241 gggttcttgt aatgatcctt tctcctacct aggtattcac cctagcaata acggcgttat
      301 tattagggta tttcttcctt ctgccatagc ttgccaagtg atcgatttaa acggaaagcc
      361 tgtgtcatca atgctaaaaa ttgatgatag aggctttttt attgcagagc tgattggtaa
      421 aaaaacagat ttttcttacc gcttgttggt ggaataccct gattcaacgg tggatataga
      481 agacccttac cgttttaaaa cccatattca agagcttgat aattggttgt ttgcggaagg
      541 aaaacaactt cgcccgtacg aaaaattagg cgcacattta gtcacacaaa atgaagttgg
      601 gggtgtgcat tttagcgtct gggcgccgaa cgctcagcgg gtttccgtgg ttggggattt
      661 caaccactgg aacggcttgg tacattcaat gcgattccac cccgattccg gtatttggga
      721 tattttcctg ccgaatgtga aaaaagggga gtgctataaa tttgaagtgt tggatcgaaa
      781 cggcgtgcta cggttgaaat ccgaccctta cgcctttgcc tcccaattcc gcccagaaac
      841 cgcatctatt gtttccggct tgccaagcat tgtgccagcc gagaaaacct gccgtaatgc
      901 gaatgctccc gataaaccga tttccattta tgaagtgcat ttaggctctt ggcgacgcaa
      961 cattgaaaac aattattggc ttagctatga agagcttgcc gatgaactca ttccttatgt
     1021 gaaagatatg ggctttaccc atattgaatt gctgcctatt tccgaatttc cgtttgatgg
     1081 ctcttggggt tatcaaacca ccggtattta ttcgccaacc agccgttttg gtacgcctga
     1141 gggcttgagt tactttatcc aaaaagctca cgataaaggc attggtgtga ttcttgactg
     1201 ggtagtgggg cattttccgg tagatgaaca cggcttggtt tattttgatg gaacgcattt
     1261 atacgaacat tccgatccga aagagggcta ccacaaagat tggaatacct taatttttag
     1321 ctacggcaga aatgaggtga gcaattattt gtctggtaat gcactttatt ggattgagcg
     1381 ttttgggatt gatgcgttgc gggtggatgc ggtggcgtca atgatttatc gtgattactc
     1441 acgttcagac ggcgagtgga ttccaaataa atatggcgga cgtgagaact tagaggcaat
     1501 cgacttctta cgccacacca ataatatttt gagagacgaa agccaaggcg gtgcgagcat
     1561 tgcagaggag tccacttctt ttgccggagt gtctcactca actgaaagag gcggtttggg
     1621 ctttaattat aaatggaata tgggctggat gaacgacact ttacgctata tgcaaaaaga
     1681 tccaatttac cgccgttatc accacaattt aatgactttt ggtatgacct accaatacag
     1741 cgaacagttt gtgttaccgc tttcacacga tgaagttgtt cacggcaaag gttcattgct
     1801 gaataaaatg ccgggggatt gctggcaaaa atttgcgaac ttacgagcct attatggcta
     1861 tatgtggggc tatccgggca aaaaattgct ctttatgggc aacgagtttg ctcaaggtaa
     1921 agagtggaat tttgccgaga gcttagattg gtatttactc ggggaagaag ggggcgaatg
     1981 gcataagggc gtattgggtt gggtaaaagc ccttaaccat ttatacaccc aaacccctgc
     2041 actgtttgag caagattaca atcctgaagg ttttgagtgg ttagtggtgg acgatcacga
     2101 taattcggtg tttgcttttg agcgtaaagc caaaaatggc gattcggtga ttgtggtgag
     2161 taatttcacc ccgattgtgc gtgaaaatta ccgttttggg gtgaatgaat tggcagaata
     2221 tcaggaaatt ctcaattctg atttagcttg ttattgcggt agtaatgtca gcaatgcaaa
     2281 ccgatttgcc agcgagttaa ttgcatcgca cggtaaagcc tactccttga gcattacttt
     2341 gccaccgcta tcgactatct tcctcaaaag agccgcagac aaaaaaacaa aacaaaacaa
     2401 aacataactt aaagaaacat aaaaaataat atgcactatt ttagaggtaa gccttttcct
     2461 ttaggcaata aacttgatag acaagcgggt gaatttggca caaattttgc cattgtttcg
     2521 caaaatgcaa ccgctattga gttatgtctt tttgatgaaa atcaccacga aacccgtatt
     2581 ccgatgtttt cttccggaga tacttggcac atttttgtag caggtgtaca agcagggaca
     2641 aaatatggct acagggtgaa aggcatacaa gatgaacaag ccggtttgtt atttaatccg
     2701 caaaaattat tgactgatcc ttacgctaaa gcgattgaag ggaagccgga tttaagtagt
     2761 gcagaggcag ccgcttggta taaatggaac gatgaacggg ataatgcctc tcttgcacct
     2821 aagtctatcg tgattgatga gcattttgat tgggaaaatg atagccagtt attcactcct
     2881 tgggcagaga ctattattta tgaaatgcag gtgaaaggtt ttagtcagcg gaactttcag
     2941 attccaagcg acattcgagg cacttttgcc ggtttggctc accctgtttc gatagactat
     3001 ttaaaaacct taggaatcac cgcagtagaa cttctacccg ttacttacca tattgatgaa
     3061 acccatttgc agaaacaagg gcttgtgaat tattggggct ataatgtatt aggtaatttt
     3121 gctattgatc cgaatttagc agcagataag caaaatcctc ttaaagagtt taaacagctc
     3181 gtaaaaacat tacatcaagc acatatcgaa gtgattatgg atattgtgtt taaccacaca
     3241 gctgagagtg ataaaaacgg gccgatgcta agttttagag gcattgacaa tacagcttat
     3301 tggctagata aacagggcga atacgaaaat tggactggct gtggtaatac cttaaatttg
     3361 agcaatcctt atatactctg ctgggcaatc gattgcttaa tctattgggt ggaggagtgc
     3421 catattgatg gattccgctt tgatcttgcc tcaattctag gtagaacacc gagtttttct
     3481 gaaaaagcag attttttcac agcacttaaa agccattcta aattaacaca tattaagttg
     3541 attgctgagc cttgggatat tggtgtagaa ggttatcaat tagggcaatt ccctgagcct
     3601 ttttatcagt ggaatgattg ttatcgtgat gatatacgcc gtttttggtt acaaaaccaa
     3661 ggtgattggg ggcttttggc acggcgattg gcaggaagtg atgatgtgtt tactaaatat
     3721 tccgtttcaa aaagtatcaa ttttattacg gcacacgatg ggtttacgtt acaagatctc
     3781 accagctatc agcaaaaaca taattgggca aatggtgaac aaaaccgaga cgggcataat
     3841 agtaactaca gcaataacca cggtgttgaa gggcagacag cagatgaatt ggtaaaaaaa
     3901 cagagagaaa tcaccgcttg tagtttgctt gctacgctct ttttatcggc tggtgttcca
     3961 atgcttttag ccggtgatga aatcggacat agtcaacaag gtaacaataa tagttattgc
     4021 caagataatg agatcacttg gctagattgg gaaaatatga atcacacacg gctggattat
     4081 gttcgtagtt taatccggtt gcgtaaaaaa atcgctttgc tttccaagca gaataattgg
     4141 tggcaagagg attcggtaca atggttgaat ccttctgctc agctaatgaa tattgacgat
     4201 tggcataatc aacataatgg gcttgtcgtt ttattacagg aagaatggct gcttttattc
     4261 aatgctaatc gacaacctca accgttttta ctgccacaag gcaactggaa agttgtgttg
     4321 gagcaggctc aaggtatttt gatgacagac acgcaagcgg ttacgctcaa taatatgggt
     4381 gtatgcgtac ttcaaaaaag tgatgtaatg ttcgctaata aattggagac acaatatgct
     4441 taaccaagaa tcaacggctc ttgttgcaag acaagattta accaacgata ccttagtatt
     4501 aattcttgca ggaggacgag gttctcgcct atacgaatta accgataaac gtgcaaaacc
     4561 ggcagtctat ttcggtggta gccgccgtat tattgatttt gcgttatcaa attgtattaa
     4621 ctcgaattta ttaaaaatcg gggtgattac ccaatatgca gcacactctt tgttgcgcca
     4681 tttacaacgt ggttggtcat ttttacctta tgaacgtaac caatatattg atatgttacc
     4741 ggcacgccaa cagctagatg aaaacacttg gtatcgtggc acagcggatg cggtgtatca
     4801 aaatatggcg atgatgaaat cgcactatag accaaaatat gtggtgattt tagcagggga
     4861 tcatatctat aaaatggact acactcaaat gctgaaagat cacgtcgatc aaggtgcaaa
     4921 atgtactgta ggttgtatcg aagtgcctcg ctcgcaagca acagagtttg gggtaatggc
     4981 ggtgaatgaa aagctgaaag tgaaagagtt cgtcgaaaaa ccagctgatc cacctgcaat
     5041 gccgggaaaa ccggattcct cgctagcatc aatggggatt tatgtattta atgcagatta
     5101 tctatatgaa atgctggaaa aagaagcctc tacaccggga acctcacatg atttcggtaa
     5161 agatattatt ccaaaagcag tagagcaagg tgtgctatat gcccacccat ttgaacgttc
     5221 ttgtaaaggg cgtaatgcca gtggggcaat ttactggcga gatgtcggta cgattgacag
     5281 ctactgggct gcccatatgg atttagtttc tgaagatccg gaattagatt tatatgatga
     5341 atcttggccg attcacggtc gtccgcaaca aactgcaccg gcacgttttt tctataatcg
     5401 tgcgcgtgcc agaaccctag ataactcact gattgcgggt ggttgtgtga ttaccgatgc
     5461 ggaaatcagt aattcggtct tatttaaccg tgttcacgtt gaagcggagt cggtgattga
     5521 acacacagtg attttaccgc aggtgactat cggtaaaaat tgcgtaatta agcgcgctgt
     5581 gattgacaga cattgtgtaa ttcctgatgg cttagaaatt ggtgtaaatg ccgaagaaga
     5641 tgcgaaacgt tttcgtgtca gcaaaaatgg cattgtatta gtgacacagt caatgttaga
     5701 taaattagcg ggaaaagaag ctaattcgga tattaaatat acaatttagt aagagatgat
     5761 ataatgcttc gagtttgtca tttgaggtcg caagctcgaa gtattgttat gattaaagcg
     5821 cttcaagcgg tcttattttt caactatttt acacatagaa aacggttaaa aatgaaagtt
     5881 ttacatattt gctctgaaat gtacccttta attaaaacag gcggtttagc tgatgtaatg
     5941 ggggcattgc catttgcgca acagcaagct ggtaatgatg tcagagtgtt aattccttat
     6001 tacccgaaag tcgcgcaaac tttaggggaa acggtagaag ttgctactat cggcactttt
     6061 gccggaacag tctctgttcg atttgcttat tttaatggct taggcgttta cgttatcgat
     6121 gcgcctcatt tatttatgcg tgatggtaac ccttaccacg acagcaatta tcaagactat
     6181 ccggataact ataaacgctt tgctttatta ggttacattg gggcgcaatt agcccaaggt
     6241 ttggatcatt ggtggggaac cgctcaagtt ttacacgcac acgattggca agcagggctt
     6301 gcttgtgcat atcttaaaag ttggggaagc aatgtaaaaa cgctcttcac tattcataat
     6361 attgcctata tcgggcgctt taatgccttc catttagcag agcttggctt accttgggaa
     6421 tttttccacg ccgaaggctt agaattttac ggtgaaattt cttacctcaa agcaggtttg
     6481 tattttgctg atcgcattac taccgttagc ccgacctatg ctcgtgaaat tactgaggaa
     6541 attgccggtg caggcttgca tggtttactg gcaacccgtc aatatcaagg tagattgcgt
     6601 ggcattttaa acggggtcga tgctgaggtg tggaatccgg aaacggatac acatattgtg
     6661 gtgaattatc gtgcgaacta tatgcacggt aaagccaaaa ataaagcgga actccaacga
     6721 atttttggtt tgccagaaga caaagaagca ttattgatgg taatggtgac tcgtttaact
     6781 gagcagaagg gggcagattt cttactcgcc aatatagata ccttaatgca acataaggta
     6841 cagattattg tattgggaag tggatcgcca gatttagaaa atgcgctacg ttatttccaa
     6901 cacaaatacc cgaaccaaat tggggtgaaa atcggttatg acgaggcttt atcgcaccaa
     6961 attattgcag gtggtgatct gattctagta ccaagccgtt ttgagccgtg cggattaact
     7021 caactttatg ggctgaaata tggcacttta ccgctggttc gccgtaccgg tggcttagct
     7081 gatacggtgg tagatagcca taaagagagc attgaaaacc gcagtgccac cggatttgta
     7141 tttgattacc cagatgcacc tgcgttttta gaggcagcta tgcgggcaat cagcttatgg
     7201 cagaaacaaa aattgtggtc gagcgttcgc cagaatgccc ttgctcaaga ttttggttgg
     7261 gcaaaagtgg cagaacagta ccaaaattta taccacgaaa ttatttaatt tgtattcaca
     7321 acatcatttc aagcggtcga atttgcggat ttttttgtaa aaacgaccgc ttgcaatatt
     7381 aaaaattagg agaatttact caatgagcag gcagtcatta agcccagaaa tgatcaaaaa
     7441 tgttaaaaat gcgattttgc acaaaatggt gttcgcttta ggggtcgaac cccgtgatgc
     7501 cagtaagcgt aactggctca atgcaacctt acgtgtagtg cgtgatttat ctaccgaaga
     7561 ttggttacaa acccgccgta gccaggttgc aaatggtagc cgccgtgttt attacctttc
     7621 aatggaattt ttaatggggc gtacgttcag taacgcaatg atttctgaag gtgtgtatga
     7681 gcttattggc gctgcgttaa aagagttagg gcaagatctg gaagagatta tcaatgaaga
     7741 gggcgtccgg gcttaggtaa cggtggctta gggcgtttag ccgcctgcta tatggacagc
     7801 cttgccacaa tgaaaattcc ggcaatcggc tacggtattc aatatgaata cggtatgttc
     7861 cgccaagaaa tccgcaacgg ggagcaaatt gagcaacctg atgaatggtt agagaatgaa
     7921 tttgtgtggc catatctacg ttcaagtaag cgtttcccgg tacgctttgg cggtaaaact
     7981 tggcgtgagg gcaaaaaagt agtatggcag ccggaagaag aaattaccgc tcaagctcac
     8041 gaccaactga tcccgggctt tgagaccacc tcaactaaca gcttacgttt atggtctgca
     8101 cacgcatcgg gtagagggtt tggcttatct gattttaacc gtggcgatta ttttgcagca
     8161 atggttaaac aaaacagctc ggaaaacgtc tctcgtgtgc tttatccgga cgattctacc
     8221 tataacggtc gtgaattacg cttacgccaa gagtatttcc tttgctcggc atctgtacaa
     8281 gatattatcc gccgccacga agcggagttc ggctcttgtg tgaatttagc cgacaaagtg
     8341 gcaactcact taaatgacac acacccaacc cttgcggtgc ctgagttaat gcgtatttta
     8401 attgacgaaa aaggctatgg ctgggaacaa gcgtgggcga tgactcgtaa aattttcttc
     8461 tacaccaatc acactttgat gagtgaagcg ttagaaactt ggccggtaga aatgttagcc
     8521 cgtgtgctac cacgccattt agagattatt ttcgacatta acgaatattt cttagaggaa
     8581 gtgcgtagcc aattcccgga agatcacgat ttgatttctc gtgtttcctt aattgatgaa
     8641 caaggcgatc gccgtgtgcg tatggcgtgg cttgcggtag tcgcctcagc gaaagtaaat
     8701 ggtgtggcga aaattcactc ggatttaatg gttgaatcta tttttgccga ttttgcccgt
     8761 atttacccaa atcgttttac taatgtgact aatggggtga cacctcgccg ctggatcaaa
     8821 attgccaacc cagggcttgc taatatttta gataaatgta tcggcaataa gtggctgact
     8881 gatttaagtg aattggaaaa attcaacgtg tttgttgatg atgctgatgt gcaagcacag
     8941 gtggcagcgg tgaaagtaga aaacaaacgt cgcttagcca aatatgtcga agaaactcag
     9001 ggaattaaac ttaatcctga agcgattttt gatgtacaag ttaagcgtat tcacaagtat
     9061 aaacgccagc aaatgaacgt gttgcatatc atcacgcttt ataaccgcat cttgaaaaac
     9121 ccgactgctg attggacacc tcgtgtgttt atcttcgcag ggaaagcggc aagtgcttat
     9181 tatgcggcga aaaaagtgat tcgtttgatt aatgatgtgg caaatgtggt gaataatgac
     9241 cctcgcatta atgatttaat taaggtggtg tttattccga actacggtgt ttcattagca
     9301 caaatgatta ttccggcagc tgatgtttca gagcaaattt ccttagcggg tacagaggcc
     9361 tccggaacct ctaatatgaa atttgccctc aatggtgctt taaccattgg tacattagac
     9421 ggtgcgaatg tcgaaatttt agatcacgtt ggtaaagaga acatctttat tttcggtaat
     9481 gaagtagagc aggtggaaga gcttcgccac aacggttact caccgtatca ctactacgaa
     9541 aaagacagcg aattgaatga ggcaatttca caaattctaa acggtaaatt ctcaccggaa
     9601 gatccgtatc gctatcaaga tttgatctta aattcaggtg actactacca agcctgtgcc
     9661 gattatcgta gctatgtgga ggctcaagaa aaagtggctc aagcctatcg taacaagaaa
     9721 gcttggacac gttcagccat catcaatatt gccaatatgg gctatttctc ctcagaccgc
     9781 tcggtgatgg attatgcgaa aaatatttgg catatcgaac cgatgagcga agaacaatta
     9841 aaaggcgatt ctacaatggg cagtattatt gaaagttcaa ataaactgtt gaatttaaaa
     9901 taatgattta gagaaaacgg taagagtaat cttaccgttt ttgttttagt cagggatttt
     9961 gcaaatttct actaaaaatt aaccgcttgt caatttgcct tctccaccgc cgtgagcatt
    10021 cccctcagta aattcagctc attagtttcc agttcggcac gttggtataa acggcggagt
    10081 tttagcatca ctgaatcatt gcggataaag cctaattcgg tatataagcg ttcggtgtga
    10141 gcaaagaaat gctctaaggc ttgagcggtt gggtaatcct tatctgctgc aagcggttgt
    10201 ttttcgccag aattttgcaa ataagccatt cggatttcat aacttgcgag ctgtactgcc
    10261 attgccaaat tcagagaacc ataatcagga ttggttggga aattcaaatg gtactggcat
    10321 ttcaataatt cttcgttggt tagccccact cgctcacgtc caaatacaat cgccactttg
    10381 ccaagattgg ttcgatttac cactaattca ccacagtctc ttggttcaat cagggtattt
    10441 tgcaaatggc gaagtctggc actggtgccg atcaccagtt ggcaatcggc aatggcttca
    10501 tcgaaagtgg cgtagcattg agcgttatcc aacacatctt ttgccccagc agcaagggct
    10561 tcggcttgtt catcaagcgg ttgttttggg gcgactaatc gcaaatgggt taagcccatc
    10621 gttttcattg ctcgggctgc ggaaccgata tttgccggga gtgaagtttc aacaagaatg
    10681 atctgaattt ggttgagaat ctgcattggt tttgtttctt ttttgttaaa tttagcctgt
    10741 taatagtgta gcaaaaattt agcctgctta aaatttgaaa aaatgggaaa taatcggttt
    10801 aattgggcat aattttttac ctatttgttt tatttagtgg tacatttaga gcgtttttaa
    10861 tcacaacaac catacaggag tctttatgct tattggtgta ccaagagagc tgttagacgg
    10921 tgaaactcga gtggcggcaa cgccaaaaac cgttgagcag atcaaaaaac ttggctttga
    10981 cgtacttgtg gaacaagacg caggttttaa agccagtttt gaagataata catttttaaa
    11041 tgcaggtgca aatatcggca cacaacaaga agtttggaat gcagacatta tttttaaagt
    11101 gaatgcgccg acagaagctg aaattgagtt aatcaaagaa ggggtaacct tagtcagctt
    11161 tatttggccg gcacaaaatc cacaattgat ggaaaaattg cagtctaaga aaattaatgt
    11221 gttggcaatg gacgctgtgc ctcgtatttc ccgtgcccaa gctctcgatg ctttaagttc
    11281 gatggcaaat atttcgggtt accgtgcggt gatcgaagcg gcaaacgctt ttggtagctt
    11341 ctttactggg caaattaccg cagcaggtaa agtaccaccg gcaaaagtgt tggtaattgg
    11401 agccggtgtt gctggcttgg ctgcaatcgg ggcggcaaat agcctggggg cgattgtgcg
    11461 tgcctttgac tctcgtcctg aagtaaaaga gcaagttaaa tcaatggggg ctgatttcct
    11521 cgaaattgat tttgaagaag aaggtggctc aggtgacggc tatgcgaaag tgatgtcgga
    11581 agagtttaac cgtcgtgcaa tggcgttata tgccgagcaa gccaaagagg tggatattat
    11641 tattaccacc gctgccattc cgggcaaacc tgctcctcgt ttaatcacta aagagatggt
    11701 tgattcaatg aaaccgggtt cagtgattgt tgatttagcc gctgcaaccg gtggtaactg
    11761 tgaatacacc aaagccggcg agattgtggt gaccgataat caagtgaaag tgattggcta
    11821 taccgatttt cctgcacgtt taccaacgca atcttcgcaa ctttatggta ctaacttggt
    11881 gaatttactc aaactgttag caccaaataa agatggtcaa attgagatta attttgatga
    11941 tgtagtgttg cgtggcgtaa cggtggtgcg tgacggcgaa ttaacttggc cggctccgcc
    12001 gattcaagta tctgcccaac tgcaaaaaca agcagccgca ccacaagcgg tcaaaaaaga
    12061 ggaaaaacct gcagaccctc gagtgaaata tggcgtgatg gcagcagcag gtgtgctgtt
    12121 cctatggctt gcatctgtgg ctccggcagc gtttttatcg cattttaccg tattcgttct
    12181 cgcttgtgtg gtagggtatt atgtggtttg gaacgtcagc cacgcactac ataccccatt
    12241 aatggcggta acaaatgcga tttcagggat tatcatcgta ggtgcggtat tacaaattgc
    12301 ccaaccgacc ggtaatttct ttattgatat tttggcattt attgcgattt tggttgccag
    12361 catcaatatt tttggtggct ttaaagtaac gcaacgtatg cttgcaatgt ttagaaaagg
    12421 ttaaggagaa acgaaactat gtcttttgga tttgtgactg ctgcgtacat tattgcagct
    12481 attctcttta ttatgagctt agccggtctt tctaaacacg aaaccgccaa agcaggttgt
    12541 tggtatggga ttgtgggtat ggcgattgct ttagtggcaa ccattttcgg cccgcaatca
    12601 catggcacga tttggatctt aatcgcaatg gcaatcggtg gattcatcgg ggtgaaaaaa
    12661 gccctaaaag tagaaatgac cgaaatgcct gaattagtgg caattctaca cagctttgtc
    12721 ggtttagcag cggtattggt tggttttaac agctacggac ttcataccaa tgcattaatg
    12781 cctgcaaatt tagatgaaac tgcacaagcg gcatttttag cggaacaagc cactcttgcc
    12841 aatatccaca atgtggaagt gttcttaggt attttcattg gtgcggtaac cttctccggt
    12901 tcattggttg cctttggtaa actaagcggt aaattatttg gtcgtaaagt ctcttctgca
    12961 gcactgaata ttccacataa acacaaatgg aatttggcag ccattatcgt ttctgttttt
    13021 ttgatgatcg tgtttttaaa taatccgcac aatatcttcc ctgtgctgat tatgacaatt
    13081 attgcgttag tcttcggctg gcacttagtt tcttctattg gtggggcgga tatgccggtg
    13141 gtagtttcaa tgctgaattc ttattcaggt tgggcagccg cagcggcagg ctttatgcta
    13201 agcaacgatt tattaatcgt aaccggtgca ttagtgggat catcaggtgc tatcctttct
    13261 tatattatgt gtaaagcaat gaaccgctca ttcattagcg tgattgccgg tggttttggc
    13321 aatgaagttc agtctggcaa aggtgatgaa gaatacggtg aacaccgtga aacgaaagcc
    13381 gaagaagtgg cagaaatgct gaaaaatgca agctctgtga tcatcacgcc gggttatggt
    13441 atggcagtgg ctcaagcaca atatccggta gcggaaatca ccgcaaaatt gcgtgaaaaa
    13501 ggcgtgaatg tgcgttttgg tattcaccct gtggcagggc gtttaccggg gcatatgaac
    13561 gtattattgg cggaagcgaa agtaccttat gatgtagtgc tggagatgga cgaaatcaat
    13621 gacgattttg aagaaaccga tgtggtattg gtaatcggtg caaacgatac cgtaaaccct
    13681 gctgcattag acgacccatc aagccctatt gctggaatgc cggtgcttga agtatggaaa
    13741 gcacaaaatg ttatcgtgtt caaacgttca atggcagtag gctacgccgg tgtgcaaaac
    13801 ccactgttct ttaaagaaaa tacccaaatg ctatttggtg atgcaaaaga acgtgttgat
    13861 gatattttac gggcgttaaa ttaaatttaa ataaaaaagc gaacataagt tcgctttttt
    13921 gtgcttaggc taacataaat tgttaatttt ttactctaat accatcaatc cgggaagagt
    13981 cgcaaaactc tgcgatttta cggtatcatg gaaatcatct aagtaggcta gggtagcatc
    14041 ggcttctcga atagcgccat aaagggtgct atatagtcct tgctgagtta tttttcttgc
    14101 caccacatac cccctatcta aatatggttt aaccgcccaa taaggtagtg cagcaatgcc
    14161 tcgccgactt gctactagct gaataatagc aatagttaac tcactattac gccgagtaac
    14221 tttgatgcca gcagggccta atatttttcg cagtaaatct agcatatcat caggtactgg
    14281 ataggtaatt aatgtctcat ctttaaaatc ttcagcttcc catacatctt ttgtggcaag
    14341 agggtgatct ttagagcaaa tagctaccat ttcatatgaa aagagaggtt tgtatataat
    14401 accagcacta tcttcctctt cagcgacaat tgccagatct gcacgatggc ttaatagcaa
    14461 accaaccgta tcagtatgga aacccgatac gatatctaat tcaactagag gccaatgttg
    14521 acggaaagta tccattgccg gcattagcca atcaaaacag gtatggcatt ctactgcaac
    14581 acgtacctct ccgacttctc cttgtttcac tcgggctaaa tctaattcag cctcaatgac
    14641 tttgggcaaa atatcgtagg ccaacttaat taaacgttcg ccagctggtg taaagcgaat
    14701 aggggagctt tttcgctcaa atagcgtaag atcatactgt tcttccagta attttatttg
    14761 gtgtgaaagt gcggattgtg ttaaatggac acgctttgct gcaagagata cgctaccgct
    14821 ctcttttaat gcgattaatg tccgcaaatg gcggatttca agaaaaatcg gtttcataat
    14881 ttattcatct aaaagtgaaa aaagatgaaa ataattataa caaacaagcg gtcaaattct
    14941 gcaaaaaatt tgcaaaatcc aaccgcttgt atagagcagt ggcgaattat tgggttaatt
    15001 gaactaaaat gcggcgaaca ggctctgctg caccccataa taattggtca cctacggtaa
    15061 atgcagctaa gtattctcca cccatcgcta atttacgcaa gcgaccaacc ggcacgctca
    15121 atgtaccggt cactttcgcc ggggtcaatt cacgcaatgt ggtttctttg tcgtttggaa
    15181 ttactttcac ccattcattg tgggcagcaa taatttgctc gatttccgct aacggaatat
    15241 cttttttcag cttaattgtg aatgcttggc tgtggcagcg taatgctcca atacgcacgc
    15301 ataaaccatc aactggaatt gggttatcgc ttaaacctaa aattttatta gtttctgcat
    15361 aacctttcca ctcttcttta gtttgcccgg tttccggtaa taatttatca atccaaggaa
    15421 ttaagctacc gcccaatgcc gcaccgaagt tctcggttgg gaagtcttcc gcacgcattt
    15481 tagccgtaac tttacgttca atatctaaaa tagaagaggc aggatttgct aattcagctt
    15541 ttacgctatc ttctaattca cccatttgag aaagcaattc acgcatattt ttcgcaccgg
    15601 caccggaagc ggcttggtaa gttgctacag aaacccactc gaccaaatct ttctcaaata
    15661 aaccaccgat agccattagc attaggctta cggtacagtt tccgcctaca aatgttttaa
    15721 tgccgttttt taagccttct gaaatcacat gttggttcac cgggtctaat acgataatag
    15781 catcgtcttt catacgtaat gcagaagccg cgtcaatcca atagccgttc caaccggttt
    15841 cttttaattt tggatagact tcatttgtat aatcaccacc ttggcaagtt acgataatgt
    15901 ctaatttttt taattcttca atgtcaaagg cgttttttaa ttcgccggca tcttttccgc
    15961 caaaaacagg ggctttttga cccgcttgtg aagtagtgaa aaaaacaggg ttgatattgg
    16021 cgaagttatt ttcttcaacc atacgatcca ttaagaccga accgaccata ccacgccaac
    16081 cgataaaacc tacattttgc attgtgtttg ctc
//
LOCUS       DANLZC010000046        15838 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.046, whole genome shotgun sequence.
ACCESSION   DANLZC010000046 DANLZC010000000
VERSION     DANLZC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15838
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.046"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..416)
                     /locus_tag="PXE44_001117"
     CDS             complement(<1..416)
                     /locus_tag="PXE44_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5588636.1"
                     /translation="MSKNRLEQILLFLKNHNIATVEQLVKVTEASPATIRRDLIKLDE
                     EGVVIRTHGGVSLNQFIPFQPTTNEKQFKQIVEKERIAEYAVSLISPGNSILLDAGTT
                     TLCIAKKIAHMPLRVITTDLHIALLLSEYKQIELIIT"
     gene            complement(524..1750)
                     /locus_tag="PXE44_001118"
     CDS             complement(524..1750)
                     /locus_tag="PXE44_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL5588637.1"
                     /translation="MKETLLVIADDLTGANDTAVMFAESGFDTVLKTKVSALAQIHPD
                     KAQVISVSTDSRAIGEKAKELTQIAISNAIQNSIGQIYLKIDSTMRGSVKYQIEGAIK
                     AWAGLYPDVKAIICPAYPEMGRTIEAGHLYVNGVPVNETASGKDPICPVHSSSMQKLL
                     PDAQVIPCDTVDNLQASIENSEYKQIVIDAKTHEDLSMIAQVINHIGNKIIPVGSAGL
                     AQKLKNIQNQQQPERNLKLGRALIVVTSIHETSQLQVDEYISTLGGKSIVFNPSPSQL
                     INYSQSEHSLKLQLNALIRFSRDHVIIRANPAKVVNAKSANITETSKEISQHLADLSK
                     FCLDNEKFDSLILFGGDGAAALLDQMNVTEMNVQYAVVPGVPLCSIEKGNYSGITVMT
                     KSGGFGNNTLLSNIMN"
     gene            complement(1747..2745)
                     /locus_tag="PXE44_001119"
     CDS             complement(1747..2745)
                     /locus_tag="PXE44_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDL5588638.1"
                     /translation="MSTQTSKSIVPIYDGMNKIPGGAMVIPLILGSIIGTFFPEFLNL
                     GSFTTALFKSSAGPLIALLIFATGMQITVRSSGPVLAHTGVVLLFKTIIPALLVVILG
                     LWVGNDGVMGVSLLAMLTVVANSNGGIWLAFTGKYGDYRDKGAYVASAVNDGPFFVLL
                     FLGVSGLADIPLSLMLAAVIPLIIGMVIGNLDPKWTELMKPTGAIVIPFFAFALGTGI
                     NLQSIITGGATGIILGIVASLFTGFLVFIGYKTILRRGNQSGIGFAAGTTAGNAIITP
                     EIVAQADPTLAPFVQTATAQIAAAVLVSAILAPLFAAWVLKRQGGLEQDTIKGENL"
     gene            complement(2756..3757)
                     /gene="pdxA"
                     /locus_tag="PXE44_001120"
     CDS             complement(2756..3757)
                     /gene="pdxA"
                     /locus_tag="PXE44_001120"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDL5588639.1"
                     /translation="MKPILGITMGDAAGIGPEVIIKALANQRIYELAHPVVIGDRKII
                     QRALPIVHSELKLRILNNLDNFVAEYGVIDILDLNNLPEDLPFAQVDPRAGKAAYEYI
                     EEAVNLAMGGKIHAIVTAPLNKEALHAGGKPFPGHTEILATLSNTKDYSMMLTSEKLN
                     VIHVTTHVQLRKACDLITKERVATVIDLAEENARILGFKEPRIAVAGLNPHSGENGMF
                     GDEDQKEIVPAIQEAQAKGFNVTGPVPPDTLFHRAANLNEFDIVVVMYHDQGHIPIKL
                     LGFDTGVNVTVGLPFIRTSVDHGTAFPIAGKGTADSRSMTEALYLGAKMANIKYATH"
     gene            4191..5555
                     /locus_tag="PXE44_001121"
     CDS             4191..5555
                     /locus_tag="PXE44_001121"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDL5588640.1"
                     /translation="MTIKTPIQAVLASNQYFLDRQDAMESNVRSYPRKLPFAYQKAQG
                     VWVTDVEGNEYLDFLAGAGTLALGHNHPKLIQAVKDVLDSGLPLHSLDITTPLKDAFT
                     EELLSFFPKDKYILQFTGPSGADANEAAIKLAKTYTGRGNVIAFSGGFHGMTHGALSL
                     TGNLSAKNAVQGLMPGVQFMPYPHEYRCPFGIGGEAGAKAVEHYFENFIEDVESGVVK
                     PAAVILEAIQGEGGVVPAPISFLQKVREVTQKHGILMIVDEVQAGFCRSGKMFAFEHA
                     GIEPDIVVMSKAIGGSLPLAVLAIKKEFDAWQPAGHTGTFRGNQLAMATGYVSLKIMR
                     EENLAKNAQERGEYLTNALRELSKELPCIGNVRGKGLMMGIDIVDERQPKDATGAYPR
                     DCDLAVAIQKYCFKNKLLLERGGRGGNVVRVLCAVNITQAECEEFIKRFKQSVAEAVK
                     AVRG"
     gene            5645..7180
                     /gene="ddc"
                     /locus_tag="PXE44_001122"
     CDS             5645..7180
                     /gene="ddc"
                     /locus_tag="PXE44_001122"
                     /EC_number="4.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,4-diaminobutyrate decarboxylase"
                     /protein_id="HDL5588641.1"
                     /translation="MSNLEKHRQSLFCSDPKSIADYESAMNNAVKAVSDWLKEEKMYT
                     GGSIKELRAKINGFNPTQAGVGVQKSLEHLVEIFLKPSLKVHHPHSLAHLHCPTMVTS
                     QIAEVLINATNQSMDSWDQSPAGSIMEEHLIDWLRQKVGYGKGTSGVFTSGGTQSNLM
                     GVLLARDWAIANHWKKADGSEWSVQADGIPADAMQKVKVVCSENAHFSVQKNMAMMGM
                     GFQSVVTVPSNANAQMDVEALAKTLADLKAEGKIVACIVATAGTTDAGAIDDLKAIRK
                     LADEHQAWVHVDAAWGGALLLSKDFRHFLDGIELTDSITLDFHKHFFQSISCGAFLLK
                     DEANYRFIDYKADYLNSEYDEEHGVPNLVSKSLQTTRRFDALKLWFTVEALGEELYAS
                     MIDHGVYLSKEVEAYINATDGLEMLVPAQFASVLFRVVPQGYPAEFVDTLNQNVADEL
                     FARGEANIGVTKVGEHQSLKMTTLSPIATLENVRALLAQVLAEADRIKDSIVNGTYVP
                     PID"
     gene            7283..7645
                     /locus_tag="PXE44_001123"
     CDS             7283..7645
                     /locus_tag="PXE44_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HDL5588642.1"
                     /translation="MKKVLGLTLMAMALSACNSTASNGSSQANTGVAPANVISVKQAL
                     AAADDSYVTVEGTLLNKVGDETYLFKDATGQIRVEIDDEVWAGQHVGSEGNKVRLYGE
                     IDKGFNKTELDVKTLTVL"
     gene            complement(7735..8322)
                     /locus_tag="PXE44_001124"
     CDS             complement(7735..8322)
                     /locus_tag="PXE44_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5588643.1"
                     /translation="MNHLIIYAHPNPSSFNHAILEKTIAASVPNKVVVRDLYQLNFKP
                     ILDWAEFQGTLSKHYEADVAIEHQYWLEADVITLIYPLWWMGFPAILKGYLDRVLSYD
                     FAYRNGEIESIGLLTGKKMQQFVTLGNSNEKYERKGFLAAFEHTLGKGLFEFCGIRDV
                     KMHYFGNIGLKETDYTAILQQITASCSTILKEQAV"
     gene            8498..9046
                     /gene="napF"
                     /locus_tag="PXE44_001125"
     CDS             8498..9046
                     /gene="napF"
                     /locus_tag="PXE44_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDL5588644.1"
                     /translation="MTDYPNQSLPRRSFLRGQFLDSLKSQKVSQQGYEPIRPPWANLA
                     NFLEKCTACGNCIQACETQIIVKGAAGYPEIDFSRGECTFCEKCVHSCEAEVFRAVSE
                     QPWSHKIEIQAGCLLKLGTECRSCGDSCEMRVIRFRPSLGGIAEMVLNLESCNGCGAC
                     LSVCPTSAIKIQPLQTQANRDE"
     gene            9039..9305
                     /locus_tag="PXE44_001126"
     CDS             9039..9305
                     /locus_tag="PXE44_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDL5588645.1"
                     /translation="MSEKLSEQENWYVCSLVVQAKPEKLEEVKAEILKIPHTEIHGEK
                     TDEGKLVVTIESNVHLALSDRIDQVRDIKGVIVVSLISNYIDEQ"
     gene            9344..11842
                     /gene="napA"
                     /locus_tag="PXE44_001127"
     CDS             9344..11842
                     /gene="napA"
                     /locus_tag="PXE44_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDL5588646.1"
                     /translation="MELNRRDFMKANAAVAAAAAAGIMIPVKNVQADDTGIRWDKAPC
                     RYCGTGCSVLVGTKDGKVVATQGDPDAEVNRGLNCIKGYFLSKMMYGADRMQEPMLRM
                     KDGKFDKNGDFTPVSWDQAFSIMAEKIKTTIKNDGPNAVGMFSSGQTTIFEGYAKVKL
                     WKGGFRSNTIDPNARHCMASAAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMH
                     PILWSRISDRRLSDNKVKVVVMSTYEHRSFELADTPIIFKPHSDLAILNYIANYIIQN
                     DKVNWDFVNKHTKFKRGETNIGYGLRPEHKLQQNTNAKTAGKMYDSDFEEFKKIVAPY
                     TLEEAHRISGVPKDQLETLAKLYADPNQKLVSFWTMGFNQHTRGVWVNHMMYNVHLLT
                     GKIALEGCGPFSLTGQPSACGTAREVGTFIHRLPADMVVTNPKHREIVENSWKLPAGA
                     IPDVPGFHATAQSRALKDGKMKFLWQMCTNNMQGGPNINEEIFPGWRNPETFIVVSDP
                     YPSVSAVAADLILPTCMWVEKEGAYGNAERRTQFWRQQVKGPANARSDTWQLVEFSKY
                     FKVEEVWPEELLAKAPELRGKTLYEILYRNGKVDSYQVPTNIPGYMNDEADHFGYYIQ
                     KGLFEEYAEFGRGHGHDLADFDTYHKVRGLRWPVVKDEKTGEYKETLWRYREGYDPYV
                     KAGEEVAFYGNADKKAVILGVPYEPPAEAPDEEYDLWFCTGRVLEHWHTGTMTRRVPE
                     LHKAFPTNLVWMHPNDAKKRGLRHGDKVKVISRRGEYVSNLDTRGRNKCPEGLIYSTF
                     FDAGQLINKVTLDATDPISFETDFKKCAVKVVKA"
     gene            11931..12836
                     /gene="napG"
                     /locus_tag="PXE44_001128"
     CDS             11931..12836
                     /gene="napG"
                     /locus_tag="PXE44_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDL5588647.1"
                     /translation="MKMDPNRRQFLKNATRTAAGVCGVGMILGLQQQQSFARQGVALR
                     PPGALPEQDFLSACTRCGQCVQACPYDMLHLASLISPVEAGTPYFIARDKPCEMCLDI
                     PCMNACPTGALSEELKDIDDARMGLAVLLDHETCLNWQGLRCDVCYRVCPLIDKAITL
                     EKVHNDRTSIHAKLIPTVHSDACTGCGKCEQACVLEEAAIKVLPMDLAKGLLGRHYRL
                     GWQEKQNAGKSLIEDVHPEGLRPAYDARMPEGLHEPVYQHMEVKPDVKVATPSRATQN
                     YVPNSTTVEAPEHFPDLDLNLKGVK"
     gene            12836..13714
                     /gene="napH"
                     /locus_tag="PXE44_001129"
     CDS             12836..13714
                     /gene="napH"
                     /locus_tag="PXE44_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDL5588648.1"
                     /translation="MAANSPKNAGLEARQKLGWWHAYRFLILRRLSQLSIILMFLSGP
                     LWNVWILKGNYSGSLLLDTVPMTDPLMTAEVLATGYRPEWTVLVGALIMVAFYGIVGS
                     KLFCSWVCPMNMVTDTAAWLRRKLGIRQTAKIPRNLRYVILAVILVGSAISGTLLWEW
                     INPVAALGRVFVFGLGATLWLVLVVFLFDLLVAEHGWCGHLCPIGATYALIGAKSLIK
                     VKVVDRNRCDNCMDCYNVCPEPQVLRVPLHGKPEESTVILSKDCITCGRCIDVCAENV
                     FAFGMRFQGSIDVKNI"
     gene            13760..14188
                     /locus_tag="PXE44_001130"
     CDS             13760..14188
                     /locus_tag="PXE44_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDL5588649.1"
                     /translation="MRKYLAFILAVMSGFAMAEAPKLSGSIDETTESVAPGFHNPQKD
                     VGTIPTTFPFQPPLVPHSIRGLQVSKNVNHCLGCHAIEPSKTTGATRLPPSHFTDRDG
                     KVYENESPRRYFCLQCHVQQTDVNPIIQNKFESVRESQAK"
     gene            14199..14807
                     /locus_tag="PXE44_001131"
     CDS             14199..14807
                     /locus_tag="PXE44_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL5588650.1"
                     /translation="MLRRFCNWLRSPSKMAIGAVILISALGGIFAWSGFNAGLAMTNT
                     EEFCSDCHMNDVVPEYRASAHYSNRSGVKAICSDCHVPHEFFPKWQRKIIAAKEVYAH
                     FTGKVDTKEKFEAHRGEMAEREWARMKANSSQECRNCHNFDDMDFTQQKNIAQQMHAL
                     AQEQGKTCIDCHKGIAHNLPHMEAIQKNFIPDDLLKKAEEKK"
     gene            complement(14869..15711)
                     /gene="cpdA"
                     /locus_tag="PXE44_001132"
     CDS             complement(14869..15711)
                     /gene="cpdA"
                     /locus_tag="PXE44_001132"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDL5588651.1"
                     /translation="MDAKRMHEYIKLPVTGVIKLLQITDPHLFSNPEETLLNVKTVKS
                     FSAVIEQINKQAKQYFDLVLATGDLIQDNNIAGYHYFAQITNSLNSPIVWLEGNHDVQ
                     PSMSEILAQYKHILPHKQILLGEQWQLLMLNTQVVGMPYGELSYSQLKWLEEKLAQYP
                     QRFTLVALHHNILPTNSAWLDQHSLKNSNELSEIFAKFKNVKAIVHGHIHQQVDDIWH
                     GIPIYATPSTCIQFKPNCDQFTLDLQPQGWREIELHENGTIETVVKRLNSNDFLPNVN
                     AIGY"
BASE COUNT         4707 a         3094 c         3464 g         4573 t
ORIGIN      
        1 gtaataatta attctatttg cttatattca gagagtaata aagcaatatg tagatcagtt
       61 gtaatcactc gaagtggcat atgagcgatt tttttcgcaa tgcatagcgt tgtagtgcca
      121 gcatctaata aaatagaatt tcccggtgaa ataagcgaaa cggcatattc agctatgcgt
      181 tctttttcga caatttgctt aaattgcttc tcatttgttg ttggttgaaa cggtataaat
      241 tggtttaagc tgacccctcc gtgtgtgcga atcacgacgc cttcttcatc taattttatt
      301 aaatcacggc ggatagttgc aggagaagcc tcagtcactt ttaccagttg ctctacggta
      361 gcaatattgt ggttttttag gaatagtaaa atttgttcta gacggttttt agacatataa
      421 actctctaat ttttaactgc ttttactata agtaattttt taaaaataag atagttaaaa
      481 ttaaagaacc taaaacattg ttgctttagg ttctttgttg ttactagttc attatattgc
      541 ttaagagtgt gttgtttcca aatcctccag atttagtcat tacagtgatg cctgagtagt
      601 tgcccttttc aatggagcat aaaggaacgc ccggtactac cgcatattgt acattcatct
      661 ctgttacatt catttgatct aataatgctg ctgcaccatc tccaccaaat aatataaggc
      721 tatcaaattt ttcattatca aggcagaatt tacttaaatc agctaaatgt tgagaaattt
      781 ctttagaagt ttccgtgata ttggcagatt ttgcatttac aacttttgca gggtttgccc
      841 gaataataac atgatctcta ctaaaacgta ttagagcgtt taattgtagt ttcagtgaat
      901 gttctgattg cgagtagtta atcaactgtg aaggcgaagg attaaataca atagattttc
      961 ctcctaaagt tgaaatatat tcatcaactt gcagttggct tgtttcgtga attgaggtta
     1021 cgacaattaa tgctctacct aatttaagat ttcgctctgg ttgttgttga ttttggatat
     1081 ttttcaactt ctgtgccaat ccggctgagc caacaggaat aattttatta cctatgtgat
     1141 ttatgacttg ggcaatcatt gagagatctt cgtgcgtttt agcatcaata acgatctgtt
     1201 tatattcact attttcaata gatgcctgca aattatcgac cgtatcacac gggattactt
     1261 gagcatcagg caatagtttt tgcatagaag atgaatgcac agggcagata ggatcttttc
     1321 ctgaagcagt ttcatttaca ggaacaccat taacatataa atgaccagct tctatagttc
     1381 ttcccatttc tggatatgca ggacaaataa ttgctttaac atcgggatat aaaccagccc
     1441 aagctttaat agcaccttca atctgatatt taacagagcc tctcattgtg gaatcaattt
     1501 taaggtaaat ttgcccgata ctattttgaa tagcattgct aattgcaatt tgagtcagct
     1561 cttttgcttt ctctccaatt gctcttgaat ctgtagaaac agaaattact tgggctttat
     1621 caggatgaat ttgggctaat gccgaaactt tagtttttaa gacagtgtca aatcctgatt
     1681 cagcaaacat aaccgcggta tcatttgcac cagtaaggtc atcagcaata actaagagtg
     1741 tctctttcat agattttctc cttttatagt gtcttgttct aagccacctt gacgttttaa
     1801 aacccacgct gcaaataatg gagctaagat tgctgaaact aatacagctg cggcaatctg
     1861 tgcagtcgca gtttgaacaa aaggtgctaa tgtagggtct gcttgagcaa caatttcagg
     1921 tgtaataatg gcatttccag cagtagttcc tgctgcaaac ccgattcctg attgattacc
     1981 acgtcttaaa attgttttgt aaccgataaa tacaagaaaa cctgtaaaga gagaagctac
     2041 gatacctaaa ataattcctg ttgctcctcc agtaataata gattgcagat tgattcctgt
     2101 tcctaatgca aaagcaaaga aaggaatgac tattgcacct gttggtttca ttaactctgt
     2161 ccatttagga tctaagttac caataaccat accaataata agaggaataa cagctgcaag
     2221 cattaatgaa agaggaatat ctgctaatcc tgatacacct aaaaatagta aaacaaagaa
     2281 aggtccatca ttaacagccg aagcgacata agctccttta tctctataat caccatattt
     2341 ccctgtaaac gctaaccaaa taccaccatt tgaatttgca acaacagtta gcatagctaa
     2401 taaagaaacg cccataacac catcattacc tacccataat cctaaaatca caactaaaag
     2461 agcgggaata atagttttaa atagtaatac aacaccggta tgggcaagta caggtccaga
     2521 acttctcacg gtaatttgca tacctgtagc gaaaattaat agtgcaataa gtggtcctgc
     2581 tgatgatttg aataaagccg tcgtaaatga acctaaattt aaaaattcag gaaagaatgt
     2641 accaataata gaaccaagaa ttagcggtat taccattgca ccgccaggaa ttttattcat
     2701 accatcatat atcgggacga tggatttaga tgtttgagta gacatattaa tctccttagt
     2761 gtgtcgcata tttgatattt gccatttttg cacccaaata gagtgcttca gtcatactac
     2821 gactatctgc tgttcctttg cctgctattg ggaaagcagt tccgtgatcg acagatgttc
     2881 gaataaatgg aagaccgaca gtaacgttta cacctgtatc aaagcctaat aatttgatag
     2941 ggatatgccc ttgatcgtga tacatcacaa caacaatatc gaattcattt aggttagctg
     3001 cacggtgaaa taaagtatcc ggagggacag gccctgtaac attaaaccct ttggcttgag
     3061 cctcttgaat tgcgggaaca atttcttttt gatcttcatc gccaaacatt ccattttcac
     3121 ctgaatgagg atttaaacca gcgactgcaa tacgaggttc tttaaaccca agaatgcgag
     3181 cattttcttc tgctaaatca ataactgtag caactcgttc tttggtaatt aaatcacagg
     3241 ctttacgaag ttggacgtga gtagtaacgt gaataacatt gagtttttca ctggttagca
     3301 tcattgagta atctttagta ttactgaggg ttgcaaggat ctctgtatgg ccaggaaatg
     3361 gtttaccacc tgcgtgtagg gcttctttat tcagtggtgc tgtaacaata gcgtggattt
     3421 ttcctcccat tgctaaatta actgcctctt caatatattc ataagctgct tttccagcac
     3481 gcggatcaac ttgagcaaaa ggcaaatctt caggcaggtt attcaaatct aaaatatcaa
     3541 taacgccata ttcggctaca aagttatcta gattattgag aattcttaat ttcaattctg
     3601 aatgaacaat aggtaaggct cgttgtataa tttttcgatc accgatcaca acagggtgtg
     3661 ctaactcata aatccgttga ttagctaatg ctttaataat aacttctggt ccgataccgg
     3721 cggcatctcc catcgtaatt cccaatatag gtttcatatt tactccttga ttgaaattaa
     3781 tcattttgaa cgaagtatag cttatatgaa atcataaaag tgtgataaac atcacaaaag
     3841 tgaacttttt taatcttatt gatttaattt aatcaatttt aaggtggtaa attggatagg
     3901 gtatgaaagt gaaaggcatt tattgctaga taaaagcttc ccttttcaat aaaaaagagt
     3961 atcatacgct ctcttaaacg taaccctaca tttaagtctg tttccaacct atcccaattt
     4021 ctttattcct taggggcata tagggtaaaa acaaaaaagc tgaaacgctt tttttaataa
     4081 gtagcgagtc aaatgcccca tggcatctat tcttttagga atagaaagct taggctttcc
     4141 gcaaggcacc cgacttattt ttttcacctc ttgtagaagg tttattttca atgacaatta
     4201 aaactcctat tcaagccgtt cttgcttcaa accaatattt tttagatcgc caagatgcaa
     4261 tggaatcaaa cgtgcgtagc tacccacgta aattaccttt tgcttaccaa aaagcacaag
     4321 gcgtttgggt aactgatgtt gaaggcaatg aatacctcga tttcctagca ggtgcaggaa
     4381 cgcttgcatt aggtcataac caccctaaat taatccaagc agttaaagat gtattagaca
     4441 gtggtttacc tttacactcg ttagatatta cgactccgtt aaaagatgct tttacggaag
     4501 aattactctc tttctttcca aaagataaat atatcctcca atttactggc ccgtctggtg
     4561 cagatgctaa cgaagcggca attaaattag ccaaaaccta cactggtcgt ggcaacgtaa
     4621 ttgctttctc cggtggtttc cacggaatga cccatggtgc gttatcgctc accggtaatt
     4681 taagtgcgaa aaatgcagta caaggcttaa tgccgggcgt acagtttatg ccttatccgc
     4741 acgaataccg ctgtccgttt ggcattggtg gcgaagccgg tgcaaaagca gttgaacatt
     4801 attttgagaa ctttattgaa gatgttgaaa gtggtgtggt aaaaccggca gcggtgatcc
     4861 ttgaagccat tcaaggtgaa ggcggtgtgg ttccggctcc aatcagcttc ttacaaaaag
     4921 tgcgtgaagt cactcaaaaa cacggcatct taatgattgt ggacgaagtt caagctggtt
     4981 tctgccgttc agggaaaatg tttgcctttg aacacgcagg cattgagccg gatatcgtag
     5041 taatgtcaaa agcgattggt ggtagcttac cgcttgcggt attagcgatc aaaaaagagt
     5101 ttgacgcttg gcaaccggca ggtcataccg gtacattccg tggtaaccaa ttagcaatgg
     5161 caaccggcta tgtatcgctt aaaattatgc gtgaagagaa tttagcgaaa aacgcacaag
     5221 agcgtggcga atacttaact aacgcattac gtgaattaag caaagaatta ccttgtattg
     5281 gcaatgtgcg tggtaaaggc ttaatgatgg gaattgatat tgttgatgaa cgtcagccaa
     5341 aagatgcaac aggtgcatac ccgcgtgatt gcgatttagc cgttgcaatt caaaaatact
     5401 gctttaaaaa caaattgtta ttagagcgtg gcggacgtgg cggtaatgtg gtgcgtgttc
     5461 tttgtgccgt gaatattacc caagcagaat gtgaagagtt tattaaacgc tttaaacaat
     5521 ctgttgctga agcagtgaaa gcggtgcgtg gttaatagtt agaatggtag gagcgaaaca
     5581 tttttcgccc taatgtaaaa aggggcgaat atttattcgc ccctacacca ataggataga
     5641 aataatgtca aacctagaaa aacacagaca atccctgttt tgtagcgatc caaaatcgat
     5701 tgcggattat gaatcggcaa tgaataacgc tgtgaaagcg gtttcagact ggctgaaaga
     5761 agaaaaaatg tacaccggtg gctcaattaa agagttgcgt gccaaaatca acggttttaa
     5821 cccaacccaa gctggtgtgg gcgtgcaaaa atcactggag catttggtgg aaattttctt
     5881 aaagccaagc ctcaaagttc accaccctca ttctttggcc cacttacatt gcccgacaat
     5941 ggtaaccagc caaattgcag aagtgttaat taatgccacc aaccaatcaa tggactcttg
     6001 ggatcaaagc ccggcaggtt cgattatgga agagcattta attgactggc tacgtcaaaa
     6061 agtcggctat ggcaaaggaa cttcaggagt ctttacctca ggtggtacgc aatcaaacct
     6121 gatgggcgta ttattggctc gtgactgggc gatagcgaac cattggaaaa aggcagacgg
     6181 ttcagagtgg tcggtgcaag ccgacggtat tcctgccgat gcaatgcaaa aagtgaaagt
     6241 ggtttgttcc gaaaacgctc acttctcggt gcagaaaaat atggcaatga tgggaatggg
     6301 tttccaatcg gtagtgactg taccgtcaaa tgccaatgca caaatggacg ttgaagctct
     6361 tgcgaaaacg ttggcggatt taaaagccga aggcaaaatt gtggcttgta tcgtcgcaac
     6421 cgcagggacg accgatgccg gtgcgattga tgatttaaaa gcgatccgta aattagccga
     6481 tgaacatcag gcttgggttc acgttgatgc tgcttggggt ggtgcattat tactgtcgaa
     6541 agatttccgc cacttcttag acggcattga attaaccgat tccattaccc ttgatttcca
     6601 caagcatttc ttccaatcaa tttcgtgtgg ggcattcttg ctaaaagatg aagcgaatta
     6661 ccgctttatt gattacaagg cagactacct gaattcagaa tatgacgaag aacacggcgt
     6721 gccgaattta gtgtcaaaat cattgcaaac tacccgccgt tttgatgcgt taaaattatg
     6781 gtttaccgtt gaagccttgg gtgaggagct atacgcttca atgatcgacc acggggttta
     6841 tctttctaaa gaagtggaag cctacattaa tgccaccgat ggcttggaaa tgttagtgcc
     6901 ggcacaattt gcctcagtct tattccgtgt cgtaccacaa ggttacccgg cagaatttgt
     6961 ggatacctta aaccaaaatg tggcagatga gttatttgct cgtggcgaag ctaacattgg
     7021 tgtgaccaaa gtcggtgagc atcaatcact gaaaatgact actttaagcc cgattgcaac
     7081 gcttgaaaac gtaagagcat tattagctca ggtgttggca gaagcagatc ggattaaaga
     7141 ttcgattgta aatggaactt atgttccgcc aattgattaa actgcgttaa aaataaaaag
     7201 gtgttttcga catttatttg tgttttaatg tttgcattcg gtagtcttta tcgaatgcaa
     7261 atttttttaa caaggagttt ttatgaaaaa agtcctgggt ttaacattga tggcaatggc
     7321 gttatcggcg tgtaattcaa ctgcttccaa cggttcttca caagcaaata caggtgttgc
     7381 accggcgaat gtgattagcg tgaaacaggc gttagcggca gcagatgaca gctacgtgac
     7441 ggtagaaggc acactcttaa acaaagtcgg tgacgaaact tacttattta aagatgcgac
     7501 agggcaaatc cgtgttgaga ttgacgatga agtatgggca ggtcagcacg ttggtagcga
     7561 aggcaataaa gtccgtttat acggagagat tgacaaaggt ttcaataaaa ccgagttaga
     7621 tgttaaaaca ctcactgttc tgtagaagat caaaccttaa tttggttccc cgccttgttt
     7681 ggcggggttt ttacgttaat taatcaaacc atccttttgc aaaaaaacgc aaaattatac
     7741 cgcttgttcc ttcaatattg tgctacaaga tgctgtgatt tgctgcaaaa tagcggtgta
     7801 atcagtctct tttaaaccaa tattaccgaa atagtgcatt tttacatcac gaatcccaca
     7861 gaactcaaac agcccttttc ccagcgtatg ttcaaatgct gctaaaaagc cttttcgttc
     7921 atatttttca ttggaattac cgagcgttac aaattgttgc atttttttgc cggtcagcaa
     7981 gccgatactt tcaatttccc cgttacgata ggcaaaatca taacttaaca ccctgtctaa
     8041 atagcctttt aaaattgccg gaaagcccat ccaccaaagc gggtaaatta acgtaatcac
     8101 atcagcttca agccaatatt gatgctcaat tgccacatct gcttcatagt gtttactgag
     8161 tgtgccttga aattccgccc agtctaatat cggcttgaag ttgagttggt agagatctcg
     8221 tacaaccacc ttatttggaa cggaagctgc tatcgttttt tccaaaatcg catggttaaa
     8281 gctggaggga ttcgggtgtg cgtaaataat caggtgattc atagcgtttt cctttactgt
     8341 gtcgaggcag ctactagaag attttagcga aactttcttt gatgaaaatc aatattttgg
     8401 cgaatatacc ggcaaatacc ccgtttgagg aatgaacggt tttaagtgaa cattagataa
     8461 tttagcctat tatagtgatg agtaaaaagg tgttttcttg actgattatc ctaatcaatc
     8521 acttcctcgc cgtagctttt tgcgagggca atttttagac tcgttaaaaa gccaaaaagt
     8581 cagtcagcaa gggtatgagc cgattcgtcc gccttgggct aatttagcaa attttttaga
     8641 aaaatgcacc gcttgcggta actgcattca ggcttgcgaa acccagatta ttgtaaaagg
     8701 tgctgcgggc taccctgaaa ttgattttag ccgtggtgaa tgcacttttt gtgaaaaatg
     8761 tgtgcattct tgcgaggcag aggtattccg agcagtttca gaacagcctt ggagccataa
     8821 aattgagatt caagccggct gtttattaaa gctcggaacg gaatgccgaa gctgtggcga
     8881 tagctgtgag atgagagtga ttcgtttccg cccgagttta ggtggcattg ccgaaatggt
     8941 attaaatttg gaaagctgta atggctgtgg ggcttgtttg agcgtttgtc cgacaagtgc
     9001 gataaaaatt cagccattac aaacacaggc aaacagagat gagtgaaaaa ttatccgaac
     9061 aggaaaactg gtatgtgtgc agtctcgtgg tgcaagcaaa accggaaaaa ttggaagaag
     9121 taaaagccga gattctgaag attcctcata ccgaaattca cggtgagaaa acagatgaag
     9181 gcaaattggt cgtgacgatt gaaagcaatg tgcatttagc cctttccgat cgaatcgatc
     9241 aagtaagaga tattaaaggc gtgattgtgg tgtctttaat ttctaattac attgatgaac
     9301 agtgattaat gattattttt gaagccaaac gtgggacata gttatggaac tcaatcgtag
     9361 agattttatg aaagcgaacg ccgctgtggc agctgctgca gccgcaggaa ttatgattcc
     9421 tgtgaaaaac gttcaggcag atgacaccgg tattcgttgg gataaagccc cttgtcgtta
     9481 ctgtggtaca ggttgtagcg tgctagtcgg tactaaagac ggtaaagtag tggcaaccca
     9541 aggtgacccg gatgcagaag taaaccgtgg tttaaactgt attaaaggtt atttcttatc
     9601 caaaatgatg tacggtgcag accgtatgca agagccaatg ttgcgtatga aagacggtaa
     9661 gttcgacaag aacggcgatt tcacgccggt ttcttgggat caagccttta gcattatggc
     9721 ggagaagatt aaaaccacta ttaaaaatga cggcccgaat gctgtaggta tgttctcatc
     9781 aggtcaaaca accatttttg aaggttatgc gaaagtgaaa ctttggaaag gtggtttccg
     9841 ttcaaatacg attgacccga atgcccgcca ctgtatggcg tccgctgcgg ttgcctttat
     9901 gcgtaccttt ggtatggacg agccgatggg ctgctataac gacatcgaaa aaaccgatgc
     9961 gttcgtgctt tggggttcaa atatggcaga aatgcacccg attttatggt ctcgtatttc
    10021 tgatcgccgt ttatcagaca ataaagtgaa agtggtggta atgtccactt acgaacaccg
    10081 ttcgtttgaa ttagccgata caccgattat cttcaaacca cactcagatt tagcaatttt
    10141 aaactacatt gcgaactaca ttatccaaaa cgataaagta aattgggatt ttgttaacaa
    10201 acacaccaaa tttaaacggg gtgaaaccaa tatcggttac ggtttacgtc ctgaacacaa
    10261 attacaacaa aataccaatg cgaaaactgc cggtaaaatg tatgacagcg attttgaaga
    10321 gtttaagaaa atcgttgccc cttatacatt agaagaagct caccgtattt caggcgtgcc
    10381 gaaagatcag cttgaaacat tagcgaaatt atatgcagat cctaaccaaa aattagtgtc
    10441 gttctggacg atgggcttta accaacatac ccgtggtgtg tgggtaaacc atatgatgta
    10501 caacgtccat ttattaaccg gtaagatcgc attagaaggt tgtgggccgt tctcactcac
    10561 gggtcagccg tccgcttgtg gtactgcccg tgaagtgggt acatttattc accgcttacc
    10621 ggcagatatg gtggtcacaa atccgaaaca ccgtgagatt gtagagaatt cttggaaatt
    10681 accggctgga gcaattcctg atgtaccggg ctttcacgct acagctcaaa gccgtgcctt
    10741 aaaagacggc aaaatgaaat tcttatggca gatgtgtacc aacaatatgc agggcggccc
    10801 gaatatcaat gaagagatct tcccgggctg gcgtaatcct gaaaccttta ttgtggtttc
    10861 agatccgtac ccatctgttt cagcagtggc tgctgactta attcttccaa cctgtatgtg
    10921 ggtggaaaaa gaaggggctt acggtaacgc agaacgccgt acccaattct ggcgtcagca
    10981 agttaaaggg ccggcaaatg cccgttcaga tacttggcag ttagtagagt tctctaaata
    11041 cttcaaagta gaagaagtat ggccggaaga gttattagct aaagcaccgg aattgcgtgg
    11101 taaaactcta tacgaaatcc tttaccgcaa cggcaaagta gatagctacc aagtgccaac
    11161 taacattccg ggctatatga atgacgaagc agatcacttc ggttactaca ttcaaaaagg
    11221 cttatttgaa gagtatgcgg aatttggccg tggtcacggt cacgatttag ccgactttga
    11281 cacctaccac aaagtacgtg gcttacgttg gccggtggta aaagacgaga aaaccggcga
    11341 atacaaagaa actttatggc gttaccgtga aggctatgac ccatacgtta aagccggtga
    11401 agaagtggca ttctatggca atgcggacaa aaaagcagtg attttaggtg tgccatacga
    11461 accacctgcg gaagcaccgg acgaagaata tgacttatgg ttctgtaccg gtcgtgtact
    11521 agaacattgg cacaccggta cgatgacccg ccgtgtgccg gagttacata aagcattccc
    11581 aaccaacttg gtttggatgc acccgaatga tgcgaaaaaa cgaggcttac gtcacggtga
    11641 taaagtgaaa gtgatttcac gccgtggtga atatgtatcg aacttagata cccgtggacg
    11701 taataaatgt ccggaaggtt tgatttactc gactttcttc gatgccggtc agttaatcaa
    11761 caaagtgaca cttgatgcga ctgatccaat ttcatttgaa accgacttca aaaaatgtgc
    11821 ggttaaagtg gtgaaagctt aattgtaggg gcaaattatt tgcctcagac aagcggtcaa
    11881 atttgcaaat ttttttacaa aaacgaccgc ttacctaaga ggaacccaca atgaaaatgg
    11941 atccaaatcg tcgccagttt ttaaaaaatg caacccgtac cgcagccggt gtttgcggtg
    12001 taggaatgat tttagggcta cagcaacaac aaagttttgc ccgtcaaggg gtagcactac
    12061 gcccaccggg tgcattgccg gagcaagatt tcctgagtgc ttgcacccgt tgcggacaat
    12121 gtgtgcaggc ttgtccttac gatatgttgc atttggcatc gcttatttca ccagtggaag
    12181 ccggcacgcc atactttatt gcacgggata aaccttgcga aatgtgcttg gatattccct
    12241 gtatgaatgc ttgcccaacc ggggcattaa gcgaagagct aaaagacatt gacgatgcac
    12301 gaatggggct tgcggtgttg ctcgaccacg aaacttgtct taactggcaa ggcttgcgtt
    12361 gtgacgtgtg ttaccgtgtt tgtccgttaa tcgacaaagc gattactctt gagaaagtgc
    12421 ataacgaccg tacctcgatt cacgccaaac tgattccgac cgtgcattct gatgcttgca
    12481 ccggttgcgg taaatgtgaa caggcttgcg tattggaaga ggcagcgatc aaagtgttac
    12541 cgatggattt agccaaaggc ttgctcggtc gtcactatcg tttaggctgg caagagaaac
    12601 aaaatgccgg taaatcgctg attgaagatg ttcacccaga gggtttacgc ccagcttatg
    12661 atgccagaat gccggaaggc ttacacgagc cggtgtatca gcatatggaa gtaaaacctg
    12721 atgtgaaagt ggctacgcca agtcgggcga cacaaaacta tgtgccgaac tctacgacgg
    12781 tggaagctcc ggagcatttc cctgatttag acttgaatct caagggggtg aaataatggc
    12841 agccaattct ccaaaaaatg cgggcttaga ggctcgtcag aaactaggtt ggtggcacgc
    12901 ttaccgcttt ttgattttaa gacgcttaag ccaactcagc attattttga tgtttttaag
    12961 cggcccactt tggaatgtct ggattttaaa aggaaactac agcggtagct tattactaga
    13021 taccgtgccg atgacagacc cattaatgac cgctgaagtg ctggcaaccg gctatcgccc
    13081 tgaatggacg gtattggttg gggcgttaat tatggtggcg ttttacggca ttgtcggcag
    13141 caagctgttt tgtagttggg tctgcccgat gaatatggta acagatactg ctgcttggtt
    13201 aagacgcaaa ctcggtattc gccaaactgc taaaattcca cgcaatttac gctatgtgat
    13261 tttggcggtg attttagtcg gtagtgcgat ttccggaaca ctattgtggg agtggatcaa
    13321 cccggttgcg gctctcggtc gggtgtttgt attcggttta ggggcaacgc tttggctggt
    13381 tttagtggtg ttcttgtttg acttattggt ggcagaacat ggctggtgtg gtcatctttg
    13441 cccgattggt gcaacgtatg ccttaattgg ggcgaaaagc ctgattaaag tcaaagtggt
    13501 agatcgtaac cgttgcgata attgtatgga ttgttacaac gtttgtccgg aacctcaagt
    13561 gttgcgtgtg ccgctacacg gtaaaccgga ggaaagcacg gttatcttat ctaaagattg
    13621 cattacctgc ggacgttgta ttgacgtttg tgccgaaaat gtatttgcct tcggaatgcg
    13681 ttttcaaggc agtattgatg tgaaaaatat ttaattttta tctttttgaa aacaagaatt
    13741 ttaatggagt gaacctcaaa tgagaaaata tctcgccttc attttagccg tgatgtccgg
    13801 ctttgcaatg gcggaagccc ctaagttatc cggatcaatt gatgagacaa ccgaaagtgt
    13861 tgcaccggga tttcataacc cacaaaaaga tgtgggcacg attccaacca ctttcccttt
    13921 ccaaccaccg cttgttccac atagtattcg tggcttacaa gtgagcaaaa acgtaaacca
    13981 ttgtttaggt tgccacgcta ttgaaccatc taaaaccaca ggtgcgaccc gtttaccgcc
    14041 aagccatttt accgaccgtg atggcaaggt gtacgaaaac gaatctcctc gtcgttattt
    14101 ctgtttgcaa tgccacgtgc aacaaactga tgtaaacccg attattcaaa ataaatttga
    14161 atctgtacgt gaatctcaag ctaaataagg agtaagtaat gttgagaaga ttttgtaact
    14221 ggttacgtag cccaagcaaa atggcaattg gtgctgtaat ccttatttct gcattaggtg
    14281 gtattttcgc ttggagtggt tttaatgcag ggttagcaat gacaaatact gaagagtttt
    14341 gttcagattg ccatatgaac gatgttgtgc cggaataccg tgcctctgct cactattcaa
    14401 accgcagcgg tgtgaaagcg atttgttcag attgccacgt accgcacgaa ttctttccaa
    14461 aatggcaacg taaaatcata gccgcaaaag aagtttatgc acactttacc ggtaaagtag
    14521 ataccaaaga gaaatttgaa gctcatcgtg gcgaaatggc agaacgtgaa tgggcacgta
    14581 tgaaagcgaa cagctctcaa gagtgccgta actgtcataa ttttgatgat atggacttca
    14641 ctcaacaaaa aaacattgcg cagcaaatgc acgcattggc acaagagcaa ggtaaaacct
    14701 gtattgactg ccacaaaggg attgcacaca atctcccaca tatggaagcg atccagaaaa
    14761 actttatccc tgatgatttg ctgaaaaaag cagaagagaa gaaataactt aacgtgtttc
    14821 aatagaaaac cctcaatttc cattgagggt ttctttttag tagccaagtt agtaaccaat
    14881 ggcattgaca tttggtaaaa aatcgttgga atttaaccgc ttgacgaccg tttctatcgt
    14941 gccgttttcg tgcaattcaa tttcacgcca gccttgtggt tgtaagtcta aggtaaactg
    15001 gtcgcaattc ggcttaaatt gaatgcaagt agatggggtg gcataaatcg gaataccatg
    15061 ccaaatatca tcaacctgct gatgaatatg accatgcaca atcgctttta cattcttaaa
    15121 ttttgcgaaa atctcggata attcattgct gttttttaag ctatgctgat ccagccacgc
    15181 tgagtttgtc ggcaaaatat tgtggtgcag agcaaccaag gtaaaccgtt gcggatattg
    15241 tgccaatttt tcttctaacc atttaagctg tgagtaagag agttcgccat aaggcatacc
    15301 taccacctgg gtatttagca tcagaagctg ccattgctca ccgagtaaaa tttgtttatg
    15361 gggaaggata tgtttatatt gcgccaaaat ttctgacata ctcggctgaa catcgtgatt
    15421 cccctctagc cacactatcg gcgagtttag cgaattagtt atctgtgcaa aatagtgata
    15481 accagcaata ttgttatctt gaatcaaatc acctgtggca agaactagat caaaatattg
    15541 cttcgcttgt ttgtttattt gctcaataac tgccgaaaaa ctttttactg ttttgacatt
    15601 aagtagagtt tcctccggat tggagaaaag gtgagggtca gttatttgta ataacttaat
    15661 tacccctgtt acgggtaact taatatattc gtgcattcgt tttgcatcca ttcaaattct
    15721 agtaattttt aactggtgag attctatcag taagctaaaa gtaaaaatgt ggactatgcc
    15781 gcatttttga aaaaaagtga gattaatcaa aaaaaattta ttattttcga tagctaag
//
LOCUS       DANLZC010000047        15763 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.047, whole genome shotgun sequence.
ACCESSION   DANLZC010000047 DANLZC010000000
VERSION     DANLZC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15763
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.047"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..1820)
                     /gene="selB"
                     /locus_tag="PXE44_001133"
     CDS             complement(<1..1820)
                     /gene="selB"
                     /locus_tag="PXE44_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDL5588652.1"
                     /translation="MIICTAGHVDHGKTSLLQALTGTNTAHLPEEQKRGLTIDLGYAY
                     LPIENDILGFIDVPGHQRFLSNMLAGLGGIQHALLVISAEEGIKPQTEEHLDILHLLN
                     FQHIMIVITKADRAEETQISHLIEQVKTSYPFLAEAKTFVTSAKTQQGIEELKAHLIQ
                     LNQQHTQTHKPFRYAIDRVFNVKGAGLVVTGTTVAGKVSVGSELFLSNGKKVRVKAIH
                     AQNTPSETGVAGQRLALNIANVEKEEIKRGDWITELEPKFATDRITVQLTANQAFKEN
                     SIVHLYHFASHITGKLNLLEGQQAVKNQQFFAEVILDEPLHIAVGDKLIIRSGDDSLT
                     LAGAEVLEIHSPKRHKRTEARLALVKNLAKTTACNDFATRLDLYLQNRAMDLSLLLWA
                     EQCFAEDIAKLGFDVSSKWLFNSDFKAQAQQRVLEKMAEYHLQHQDQIGVTKARLYRI
                     ALLDLPENLANQFIEDLVEQKALVNSRGWLHLPEHRIELNADELQIWQQIRPLFEATN
                     QALWVRDIASQLSIDETQMRNLLYKVGKLGYLIPIVKDRFLLSEQIGQFATLIKDFIA
                     QNGSISVNQLRDELQYGRKLTVQLIEYFDRSGFLRRKGDV"
     gene            complement(1830..2126)
                     /locus_tag="PXE44_001134"
     CDS             complement(1830..2126)
                     /locus_tag="PXE44_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5588653.1"
                     /translation="MMKFDYNIVWLQKAEIKLFYQADYILQQSQNPIIAEQFYDAIKS
                     EVDKLSFTADVYRLRKKKEIPILNGKYQVKFLIGQENVYIVDFKSARQNSYSFL"
     gene            complement(2123..2299)
                     /locus_tag="PXE44_001135"
     CDS             complement(2123..2299)
                     /locus_tag="PXE44_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588654.1"
                     /translation="MELILAQDLAKLRTDKPQSFYTNDELDLLHQLENGLIETKSHAQ
                     VMEDLRKKLGITEK"
     gene            complement(2368..3000)
                     /gene="pdxH"
                     /locus_tag="PXE44_001136"
     CDS             complement(2368..3000)
                     /gene="pdxH"
                     /locus_tag="PXE44_001136"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDL5588655.1"
                     /translation="MDLHHIREDYTKKVLSIRDCHSNPITQFEQWLSEAINAKVNEPT
                     AMNIATVAANGRPSSRMVLLKEVNSQGFVFFTNYQSRKGWEIADNPFGCLTFFWAELQ
                     RQVRIEGKIAKIPEAESDAYFDSRPYTSRLGAWASEQSQPIENYKALLLKSAQFALKY
                     PLSVPRPPHWGGYILIPDSIELWQGRSSRLHDRIHYWLENNEWKKERLSP"
     gene            complement(3058..4440)
                     /locus_tag="PXE44_001137"
     CDS             complement(3058..4440)
                     /locus_tag="PXE44_001137"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDL5588656.1"
                     /translation="MQQLFRQIPSLDLLLKKPQAVELCQKFAYSSVAEQARSLLTQAR
                     QFISQQQALPDFIADENLFFIRLEQKVQALSEVKIRSVFNLTGTVLHTNLGRGLWSEK
                     AIQAATNAMRNNVALEFDIEAGKRSHRDNYISELLQILTGAEAACIVNNNAAAVLLML
                     ATFAQGKEVIVSRGELVEIGGAFRIPDIMAQAGCKLVEVGTTNRTHLKDYRNAITENT
                     AFLMKVHTSNYHIEGFTSSVSEEELVALGKEFNLPVITDLGSGSLTDMQALNLPAEPK
                     VQQKVAAGVDLVSFSGDKLLGGPQAGIIVGSQAMISQLQQHPLKRVLRCDKVILSALE
                     ATLRHYLFPEQLTDNLPTLHLLTQPLETLQHKAERLKQALHKRLNSAYILQIELSLAQ
                     IGSGALPTETLPSVAVTISAEKQSDLIALEQQFKTYPSPIIGRFAQQKFWLDLRSVAE
                     FDKLIEMLEY"
     gene            4508..4602
                     /locus_tag="PXE44_001138"
     tRNA            4508..4602
                     /locus_tag="PXE44_001138"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4542..4544,aa:Sec,seq:tca)
     gene            4809..5498
                     /locus_tag="PXE44_001139"
     CDS             4809..5498
                     /locus_tag="PXE44_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL5588657.1"
                     /translation="MSTLSRDSILSQIQNGLIASCQPVDDGPMDKPEIVAAMAAASVI
                     GGAKGIRIEGVDNLKAARAAVNVPIIGIVKRDLPDSPVRITPFLEDIDALAEAGADII
                     AVDGTFRTRPVSIEEAVKRIHEKGCLAMADCSTLEEGLHCQQLGFDIVGSTMSGYTGG
                     EMPDEPDYQLVKDLKAAGCNVMAEGRYNTPELAKTAIEIGADYVTVGSALTRLEHIVS
                     WFADSVKSAQK"
     gene            5508..6410
                     /locus_tag="PXE44_001140"
     CDS             5508..6410
                     /locus_tag="PXE44_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDL5588658.1"
                     /translation="MRCLAIDIGGTKIATAIVQHNQVSQRQQIQTPTDKPQADQAGSL
                     HQAIGEIMQHYQGQFDFVAVASTGIINKGVLTALNPKNLGGLAEFPLKQSIEKHTDKP
                     IALLNDVQAAVCAEYLNEDQQAVKNFVFITVSTGVGGGIILNGELQIGSNGIAGHIGH
                     TLADPNGPLCGCGRVGCVEAVAAGRAIEAVSSQWEKPCSPKEVFERFRQGNPQAVELV
                     QKSAKAIANLIADLKISLDIQKVVLGGSVGLAEGYLPQVSNYLAEMPSVYHCVLENAL
                     SGQDAGLIGAAWWAENQLNQGFTL"
     gene            6435..7313
                     /gene="nanA"
                     /locus_tag="PXE44_001141"
     CDS             6435..7313
                     /gene="nanA"
                     /locus_tag="PXE44_001141"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDL5588659.1"
                     /translation="MKNLKGIFSALLVSFNEDGTINEKGLRQIIRHNIDKMKVDGLYV
                     GGSTGENFMLSTEEKKEIFRIAKDEAKDEIALIAQVGSVNLKEAVELGKYATELGYDS
                     LSAVTPFYYKFSFPEIKNFYETIIRETGNKMIVYSIPFLTGVNIGVSQFAELFENEKI
                     IGVKFTAGDFYLLERLRKAFPNHLIYAGFDEMMLPATVLGVDGAIGSTFNVNGIRARQ
                     IFELAQQGKIKEAFEIQNVTNDLIEGILGNGLYQTIKGLLELEGVQAGYCREPMTKEQ
                     TPAQKEVTKQLKAKFL"
     gene            complement(7374..7580)
                     /locus_tag="PXE44_001142"
     CDS             complement(7374..7580)
                     /locus_tag="PXE44_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588660.1"
                     /translation="MDNKQTNGFNDIIVKADITQNIWSTGFYDIGRELKSENTRQEKV
                     RLKYNGDTLHRGRKSRMASLIASR"
     gene            complement(7538..8176)
                     /locus_tag="PXE44_001143"
     CDS             complement(7538..8176)
                     /locus_tag="PXE44_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588661.1"
                     /translation="MKKILLLVLLLTLATQADEGNNTQVCAEQQHQIIAHYFKISQEK
                     LSARTLASACKTAPNNSTETLVSYAVIEPKYSKENYQNDENTPKWTLFVARVNDNAIL
                     QSWEQDMEIDASIDVNADSLKIDTARYILNDKVRAFGVRFNNAARGPSAPEYSANDHL
                     MLFVPEKEKLKLVFDYPMTFWSILDDKETKTETQTWYCKWITNRPTASMILL"
     gene            complement(8418..9137)
                     /locus_tag="PXE44_001144"
     CDS             complement(8418..9137)
                     /locus_tag="PXE44_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5588662.1"
                     /translation="MSKPNIYDESFSERMKWIAKNSFKDNYSEFARAVGVAQASLSRW
                     VKGEADPSRSNLVKIAEVSNVSLEWLATGKESKPQSENTSIVAREFERLEKMADDIVS
                     MINSFRTINVSAGFGSFNEGVTKPDGQEPYADSLLQSLGVKAHNCGVFWANGTSMHPT
                     IADSDQMLVDFSKREPRGDDKIYLVQNGESVWVKRIRKEWDYIELISDNESYRPIQIT
                     AEEAQNLQIIGQVVHIGHSLV"
     gene            9314..9592
                     /locus_tag="PXE44_001145"
     CDS             9314..9592
                     /locus_tag="PXE44_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5588663.1"
                     /translation="MSVLNDVKKTAQRDWHKADILAELRKKGWSLRSLAKAGNVSHNT
                     LKSVLDKPYPKMERLVANAIGVAPEVIWAGRTAERIERNRRPTLTNKF"
     gene            9637..10440
                     /locus_tag="PXE44_001146"
     CDS             9637..10440
                     /locus_tag="PXE44_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5588664.1"
                     /translation="MSELSLKTHYSVAELLSFKLSSLPSAHKNVLEKAIRENWQSQKR
                     KGRGGGVEYELISLPTEVQQEIRTKLLKLLPAEISKGELSVVRQNIDLEQITDKQLST
                     ADARIMVVRWFLMQEVQLGLSRTKTLDQVIAAVASSEIPAEICKAIMAGNSKAGGKLK
                     LSKRTLHSWVLAYEAGENSAERLKQMIPLKTQKRAVPERCGWLQAFLPFYQTFSNVAL
                     TQAYAQFAMQYEGEDLPTESQVRYALKQLPDYVVQQGRKLPVKKIRLAR"
     gene            10407..10919
                     /locus_tag="PXE44_001147"
                     /pseudo
     CDS             10407..10919
                     /locus_tag="PXE44_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            10930..11304
                     /locus_tag="PXE44_001148"
     CDS             10930..11304
                     /locus_tag="PXE44_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5588665.1"
                     /translation="MSIVYEGSCVLEVDGVEIEITKLDVKTVTGRKLVKTMNSSGRAR
                     GYSQGIAEYTLSVTAVEPQDGSVIDWDNIKNAKLTKYPLDNSEKRTSYLGCFTTDVSE
                     SYTVDGESVRDIQLNALRKVNE"
     gene            11304..11672
                     /locus_tag="PXE44_001149"
     CDS             11304..11672
                     /locus_tag="PXE44_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588666.1"
                     /translation="MKITLNLGIEYNGKIRRDCTVRLLTMGGQCTAQEMIADLGFEEN
                     SEALSKREQTLVDMAYLSQQVTFDGIYQGNVTAQFLFDNLATEDYWQLIEATLTLRKK
                     RLENGVSQSESESPANPDLA"
     gene            11702..11890
                     /locus_tag="PXE44_001150"
     CDS             11702..11890
                     /locus_tag="PXE44_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588667.1"
                     /translation="MILLSKFAITAEQVWQMPLLEVFAWIESYLESEGVKTPHSPMGA
                     NSEVKRESYVFTRCGKIE"
     gene            11943..12938
                     /locus_tag="PXE44_001151"
     CDS             11943..12938
                     /locus_tag="PXE44_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5588668.1"
                     /translation="MAKNMKVQLELTAKDTASPVLNQAAKTAEKAFKNTERAAQKSGN
                     AQTQAAQKAANATESSNRKIEQSYRNARRVAADVSRARETLGVRAENAIQREIEQTRA
                     AYDRLKRSGVASQNELRRASEATKQRVRELNAELGKTSFGDKAKGFGRGLMSAGVGIA
                     AGAAMARQPAKNQMEFDRQLAMASNTAFSDRDVKGRIEGKKELLAAVQEAVKVGGGTK
                     EDALLTLDTLIASGTMKAETAMSLLPTLQKGAVATGASSEDMAKIAISSMQQFGIKEE
                     DIGRALDMAVAAGQAGNFELADMARWLPQQMAAGNTARHWAVYYRLYRMTQTADG"
     gene            12887..13444
                     /locus_tag="PXE44_001152"
     CDS             12887..13444
                     /locus_tag="PXE44_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDL5588669.1"
                     /translation="MGGLLPPVSYDPNGRWLALSLEVEGRELDRIDARAKTLIDAVDV
                     STGIFIDDWERVCGLTANSSLPLETRIERVIAKLNQLGGLSIGYITQVAKELGYCIKI
                     SKPQPFIAGLSRAGDMLWHKDIMWTFFVDVCNPTDDYKRFRASVSMAGDALMGRYKDP
                     ILESLLEEVKPAFSRVWVRYLGDIE"
     gene            13441..15501
                     /locus_tag="PXE44_001153"
     CDS             13441..15501
                     /locus_tag="PXE44_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5588670.1"
                     /translation="MKQVMNPINTPTQRFKDGNPATGEYGTIVTAEFLNNVQDSVINT
                     QQELHSVLAEAGIEANDEQVNQVAKAIKKIAGDATRDNFNALANPDGYKHIGRCKSVA
                     ELRTIRPTEHGQRILVDAYYEDGTTGGGEFVADLQDLVTPDDGGVCFVVNNNGGRWKR
                     VDLSHLTLFDFGAVGDGVTNDESAFVNAMRYSQFFIENGTFRINNAVNSVRDNVQILG
                     NKTGKLVLGAGIQQAEAEVFNINHSNYFISGFCIETPNKAIGIRFKSLDDAGVKNLHI
                     DNVVFNGTFYGVRAGESIQADTNYPTDNVIVQNCQSYCGSGNAGHYLCTKVKGVRFFN
                     NIAIGGRNVSAYGATSCSDIFILGNREQGMAVTTVDVEAGVQVEDVQTGECVVANNIC
                     SHDIWMSGAKNAIINSNLCRRLRVTSGNPTDTGSNNVEFSNNQAKSIAISKYGSFDNP
                     SLFIDNINFINNILEPSEFNQDVYIDGTILKQCQFINNRCISKSKQYSVRLARNNPCL
                     NLTFKSNKLSDNPAIISGKQGVIFCDDLSLGVNKHRLRLRFSSPINFTVNQEWQKMPF
                     DRVLNDINGHVDIVSGQFIARTGGTFHLKGKITVTNSSEDVLNLYQLRLLINNEEKER
                     LINQSVSPKNSKSNTNGMTTINFDTVVYLKVGDIVDLQYKTTHELTLLSDSFLSQIYV
                     TQEF"
     gene            15502..15744
                     /locus_tag="PXE44_001154"
     CDS             15502..15744
                     /locus_tag="PXE44_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588671.1"
                     /translation="MTKKHYCTGWRNAPANINSCCHQHDRDYGIKGTVSRAEADKRLR
                     HCLISQGNPIKAWVFWVVVRLFGWIYYKEKNINGKS"
BASE COUNT         4582 a         3194 c         3516 g         4471 t
ORIGIN      
        1 tgcacatcgc cttttcgtct taaaaaaccg ctacgatcaa aatattcaat taattgtacc
       61 gtcaattttc gcccatactg caactcatct cgcaactggt tcaccgaaat gctgccgttt
      121 tgggcgataa aatctttaat taacgttgca aattgcccga tttgttcgct taataaaaat
      181 ctatctttga cgattgggat taaatagccc aatttcccga ctttatacag caaattccgc
      241 atttgggttt catcaatcga gagctggctt gcaatatccc tcacccaaag agcttgattg
      301 gttgcctcaa acagcggtcg aatttgttgc caaatttgca attcatcggc gttcaattcg
      361 attcgatgtt ccggcaaatg taaccaacca cgactattga ccaaggcttt ttgctccacc
      421 aaatcctcaa taaattgatt tgccaagttt tcaggcaaat ccaataacgc tatgcgatat
      481 aaacgagctt tggtcacgcc gatttgatct tgatgttgca ggtggtattc tgccattttt
      541 tccaacaccc gttgctgggc ttgagcttta aaatccgaat taaataacca tttagacgag
      601 acatcaaacc ccagttttgc aatatcctct gcaaaacatt gctccgccca taacagcaag
      661 gataaatcca tcgctctgtt ttgcagatag agatcaaggc gtgtcgcaaa gtcattacaa
      721 gcggtggttt ttgctaaatt ttttaccaaa gctaaacggg cttcggtacg tttatgccgt
      781 tttggtgagt gaatttccag cacctccgct ccggctaacg ttaggctgtc atcaccgctg
      841 cggatgatca acttatcacc aacggcaata tgaagtggtt catctaaaat cacttctgca
      901 aaaaattgct gatttttgac cgcttgttgc ccctcaagca aattcaattt gccggtaatg
      961 tgcgaagcaa aatggtaaag atgcacaatg ctgttttctt tgaaggcttg attggcagtg
     1021 agttgaaccg taatgcgatc ggtggcaaat ttcggctcaa gctcggtaat ccaatcgcct
     1081 cgcttgattt cctctttttc cacgttagcg atatttaacg ccaaacgttg ccctgccact
     1141 cccgtttccg aaggcgtatt ttgggcgtga atcgctttga cccgcacttt tttaccattc
     1201 gacaaaaata actcagagcc aacgctcact tttcccgcca ccgttgtgcc ggtaacgacc
     1261 aaccctgctc ctttcacatt aaacacacgg tcaatcgcat aacggaacgg tttatgggtt
     1321 tgggtatgtt gttgatttaa ttgaattaaa tgtgctttta attcttcaat cccttgttgc
     1381 gtttttgccg aagtaacaaa ggtttttgct tccgctaaaa acggatagct tgttttcact
     1441 tgctcaatta agtggctgat ttgagtttct tccgcccgat ctgccttagt aatcacaatc
     1501 ataatatgct ggaaattcag caggtggaga atatccaaat gctcttcggt ttgtggctta
     1561 atcccttctt cggcagaaat caccaacagg gcgtgttgaa tgccgccaag ccctgccagc
     1621 atattagata aaaaacgctg atgacccggc acatcaataa accctaaaat atcgttctca
     1681 atcggtaaat aggcatagcc caaatcaatg gttagcccac gtttttgttc ttcgggcaaa
     1741 tgggcggtgt tagtgccggt taaggcttgt aaaagcgatg ttttgccgtg gtcaacgtga
     1801 ccggcggtgc agatgatcat tgatagtcct tataaaaatg agtaactatt ctggcgagca
     1861 gatttaaaat ctacaatata gacattttcc tgaccaatca ggaactttac ttggtactta
     1921 ccgtttaaaa tgggaatttc ttttttcttg cgaagtcgat aaacatcggc agtaaaggaa
     1981 agtttatcca cttcactttt tattgcatca taaaattgtt ctgcaataat cgggttttgg
     2041 gattgttgta atatataatc tgcttgataa aacaatttta tttctgcttt ctgtagccaa
     2101 acaatattgt aatcaaactt catcattttt ccgtgattcc caattttttg cgtaaatctt
     2161 ccatcacctg agcgtgggat ttagtttcaa ttaagccatt ttctaattga tgtaataaat
     2221 caagttcatc attagtataa aaactttgtg gcttatctgt gcgtaatttt gctaaatctt
     2281 gtgcgagtat gagttccata tttccccctt acaaacttag attattcaat attgtaacct
     2341 gataaaacta aagttcaagg agtgtattta aggcgataac cgctcttttt tccactcatt
     2401 attttccaac caataatgaa tgcggtcgtg tagccggcta cttctgcctt gccagagttc
     2461 aatactgtcg ggaatcagaa tatagccacc ccaatgcggt gggcgaggca ctgaaagtgg
     2521 atatttcaat gcaaattgag ctgattttag caacaaagcc ttgtagtttt cgataggctg
     2581 actctgctcg ctcgcccaag cccccaaacg gctggtgtaa ggacgagagt caaaataggc
     2641 atcggattct gcctctggaa tttttgctat tttcccttca atccgcactt gacgttgtaa
     2701 ttctgcccaa aagaaggtta aacatccaaa aggattatcg gcaatttccc accctttacg
     2761 gctttgataa ttggtaaaga acacaaagcc ttgtgaattt acctctttca acagcaccat
     2821 tcggctactt ggtcgcccgt ttgctgcaac ggtagcaata ttcattgctg tcggctcatt
     2881 cactttggca ttgattgcct cactcaacca ttgttcaaat tgggtgattg ggtttgagtg
     2941 acaatctcgg attgaaagca cttttttggt gtaatcttct cgaatgtggt gcagatccat
     3001 aaggattcct ttatcttatt ctaaataatc ggacaggcac agtctgccct accaaaatta
     3061 atattccaac atctcaatca acttatcaaa ctccgcgacc gagcgtaaat ctaaccaaaa
     3121 tttctgctga gcaaaacgcc cgataatcgg gcttgggtag gttttaaatt gttgttccaa
     3181 tgcaattaaa tcgctctgtt tttcagcgga aatagtcact gccaccgaag gtaaggtttc
     3241 tgtgggtaac gcaccgctgc cgatttgagc gaggcttagc tcaatttgca aaatataagc
     3301 agaatttaac cgcttgtgta gtgcctgttt taatcgctct gctttgtgtt gcaaggtttc
     3361 aagcggttga gtgagtaaat gaagagtcgg caagttatcg gttaattgct ccggaaataa
     3421 gtagtggcgt agcgttgcct caagtgccga taaaatcact ttatcgcaac gtagcacccg
     3481 ttttagcggg tgttgttgca gttggctaat catcgcttgg ctaccgacaa taatgccggc
     3541 ttgcggcccg cctaataatt tatcgccaga gaaggaaacc aaatctaccc cagccgctac
     3601 tttttgctga acctttggct cagcaggcaa attaagagct tgcatatcag ttagcgaacc
     3661 actgcctaag tcggtaatca caggcaggtt aaactcttta ccgagagcca ccaattcctc
     3721 ttccgacacc gaactggtga acccctcaat gtggtagtta ctggtgtgaa ctttcattaa
     3781 aaaggcggta ttttcggtga tcgcgttgcg ataatccttt aaatgagtgc gattagtcgt
     3841 accaacttcc accaatttgc aaccggcttg agccataata tccggaatgc ggaatgcccc
     3901 gccaatttcc accagctcgc cacgggaaac gatgacttcc ttgccttgtg cgaaggttgc
     3961 taacatcaac aacaccgctg ccgcattatt attcacaatg caagcggcct ccgctccggt
     4021 aagtatttgc aacaactcgg aaatgtagtt atctcggtgc gagcgcttgc ctgcttcaat
     4081 atcaaattcc aacgccacat tgtttcgcat tgcgttggtt gctgcctgta tcgccttttc
     4141 cgaccacaac ccacgcccta agttggtgtg taacaccgta ccggttaagt tgaacacgga
     4201 acggattttc acctcactca acgcttgcac tttttgttcc agccttataa aaaacaggtt
     4261 ttcatcggca ataaaatccg gcaaggcttg ctgttgggaa ataaattgac gtgcttgagt
     4321 aagcaaactc ctagcttgtt cagccacgct ggaatatgca aatttttggc ataattcgac
     4381 cgcttgtggc tttttcaaca agaggtcgag cgagggaatt tggcgaaata gttgttgcat
     4441 aatgtgaatg attgagagaa tattttggac aattctaata aataaagtac aatattgcca
     4501 atttttcgga agtgcgtcat ctccggtgag gtggcaggac ttcaaatcct gttgaggacg
     4561 ccagcgttct cgggtgggtt caactcccat gcacttccgc cacatttcaa aagccgttaa
     4621 tctttaacgg ctttttgcat ttctgccaca ttctctcttt ccgttttgat aaaaaatgag
     4681 acttagctca cattttttta tttttctgtt tgtagtaaaa atttattaaa ttataattat
     4741 ttggagtatt ttttcacaaa caaaatctag tccattgact accttttgaa cataaaaaag
     4801 gaactgttat gtcaacacta tctcgtgatt ctattttaag tcaaattcaa aacgggctga
     4861 ttgcctcctg ccaaccggtt gatgacggcc ccatggataa acctgaaatt gtcgccgcaa
     4921 tggcagcagc ctctgtgatt ggtggtgcta aaggcattcg cattgaaggt gtcgataatt
     4981 taaaagccgc tcgtgcggca gtgaatgtgc cgattatcgg cattgtaaaa cgtgatttgc
     5041 cagattcccc tgtgcgtatt acgccatttt tagaagatat tgatgcctta gccgaagccg
     5101 gtgccgatat tattgcggtg gacggcacat tccgcacccg cccggtcagc attgaagagg
     5161 cggtaaaacg cattcacgaa aaaggttgct tggcaatggc ggattgctcc acccttgaag
     5221 aaggtttaca ttgccaacaa ttaggctttg atattgtggg tagtacaatg tctggctaca
     5281 ccggtggcga aatgcctgat gagccggact atcaattagt gaaagattta aaagctgccg
     5341 gctgtaatgt tatggcggaa ggtcgctaca acacgcctga actggcaaaa acggcgattg
     5401 aaatcggggc ggattacgtt accgttggat cggcattgac ccgcttagag catattgtga
     5461 gctggtttgc cgactctgta aaatcggctc aaaaatagga gctgcttatg cgttgtttag
     5521 caatagacat cggtggcacg aaaattgcca cggcaattgt gcaacacaat caagtgtcac
     5581 aacgccaaca aattcagacc ccgactgata aaccacaagc tgatcaggca ggcagtttgc
     5641 atcaagcgat tggtgagata atgcaacatt atcaagggca atttgatttt gtggcagttg
     5701 cctcaacagg cattatcaat aaaggggtgc ttaccgcatt aaatcctaaa aatttaggcg
     5761 gattggcaga atttccgttg aaacaaagca ttgaaaaaca taccgataag ccgattgcat
     5821 tattaaatga cgtgcaagcg gcggtgtgtg ccgaatattt aaatgaagac caacaagcgg
     5881 tcaaaaattt tgtatttatt accgtttcca ccggcgtagg cggcggcatt attttaaacg
     5941 gcgaactgca aatcggcagt aacggcattg caggacatat cgggcatact ctagccgatc
     6001 cgaacgggcc tctctgtggt tgtggacgag tcggctgtgt cgaagcagtc gcagcagggc
     6061 gtgcaataga ggcagtttcc agccaatggg aaaagccttg ttcaccgaaa gaggtgttcg
     6121 agcgattccg ccaaggcaac ccacaagcgg tcgaattagt acaaaaatct gcaaaagcga
     6181 ttgctaattt aattgcagat ttaaaaatca gtttagatat acaaaaagtg gtgttaggtg
     6241 gcagtgtggg cttagcggaa gggtatttac cgcaggtgtc caactattta gctgaaatgc
     6301 cgagcgtata tcattgcgta ttggaaaatg ctctatccgg gcaagatgcc ggcttaatcg
     6361 gtgcggcttg gtgggcggaa aaccaactta atcaaggttt tactctttaa caaattctaa
     6421 ctccggagat ccaaatgaaa aacttaaaag gtatttttag tgcgttactc gtctctttca
     6481 acgaagacgg caccatcaac gaaaaaggct tacgccaaat tatccgccac aacatcgaca
     6541 aaatgaaagt ggacggttta tatgtcggcg gttcaaccgg tgaaaacttt atgctttcta
     6601 ccgaagagaa aaaagaaatt ttccgcattg ccaaagacga agcgaaagat gaaatcgccc
     6661 ttatcgccca agtcggtagc gtaaacttaa aagaggcggt agaactgggt aaatatgcca
     6721 cagaattagg ctacgacagc ttatctgcgg tcacaccgtt ctactacaaa ttcagcttcc
     6781 cggaaatcaa aaatttctac gaaaccatta ttcgtgaaac cggcaacaaa atgattgttt
     6841 actccattcc gttcttaacc ggcgtaaaca tcggtgtgag ccaattcgct gaattatttg
     6901 aaaacgaaaa aatcatcggt gtaaaattca ctgccggcga tttctacctg ttagagcgtt
     6961 tacgcaaagc gttcccgaac cacttaatct acgcaggttt cgatgaaatg atgctaccgg
     7021 caacggtatt aggtgtggac ggtgcaatcg gctcaacctt caacgtaaac ggtattcgtg
     7081 caagacaaat ctttgaatta gcccaacaag gcaaaatcaa agaagcattt gaaatccaaa
     7141 atgtaaccaa cgatttaatc gaaggcattt taggcaacgg tttataccaa accatcaaag
     7201 gcttattgga gttagaaggt gtgcaagcag gttactgccg tgagccgatg acgaaagaac
     7261 aaactccggc acaaaaagaa gtgaccaaac agttaaaagc taaattcttg taatttaagt
     7321 tgcaaaaggc taaacgaaaa tcaccgcttg tagtaacaag cggtgatttt ttctcaacga
     7381 cttgcaatta atgaagccat tctgcttttt ctaccacggt gtaacgtgtc accattatat
     7441 tttaatcgca ctttttcttg gcgagtattt tctgatttca actctctacc tatatcataa
     7501 aaccctgtac tccagatatt ttgagtaata tccgctttta caataatatc attgaagccg
     7561 ttggtctgtt tgttatccat ttgcaatacc aagtttgtgt ttccgttttg gtttctttat
     7621 catctaaaat agaccaaaaa gtcatcgggt aatcaaaaac aagttttaac ttttcttttt
     7681 ccggcacaaa taacatcaaa tgatcatttg cagaatactc aggagcactt ggaccacgag
     7741 cagcattatt aaacctcacg ccgaaagctc ttactttgtc gtttagaata taacgggcgg
     7801 tatcaatttt aagactatct gcattcacat cgatagaggc atcgatctcc atatcctgct
     7861 cccacgattg caatatcgca ttatcattca cacgagcaac aaaaagcgtc cacttaggtg
     7921 tgttttcgtc gttttgataa ttttctttgc tgtatttagg ctctattacc gcataactca
     7981 ctaaggtttc cgttgaattg ttaggagctg ttttacaggc agaagctaaa gtacgagcag
     8041 aaagtttctc ttgagaaatc ttaaagtaat gagcaataat ttgatgttgt tgctcagcac
     8101 aaacctgtgt gttattacct tcatctgctt gagttgcaag cgtaagtaaa agaactaaga
     8161 ggagtatctt tttcatatag atttatccgt atagaaagat aactcattct atggggtact
     8221 gactcttttt gcatagagag aaagaaaaaa atttggtcgg aaaaataacg gcgagcctag
     8281 cgaaaacaag ggttcgccgt aattttatgg gggaagctat gtaaaaaaca aaatgagcag
     8341 gattagaaaa aaatgagcag tttttgcgat attttttgca taggtttgat cggttttaaa
     8401 actattttaa atctgtttta aactaaagag tgtccgatgt ggactacctg cccgatgatt
     8461 tggaggtttt gagcttcttc ggcggtaatc tgtattgggc ggtaggactc gttatcgcta
     8521 attaattcaa tatagtccca ttcttttcta atccgtttaa cccatacgct ttccccattc
     8581 tgcacaagat aaatcttatc atcaccacga ggctctcgtt tgctgaaatc taccagcatt
     8641 tggtcactgt ctgcaatggt agggtgcatt gatgttccat ttgcccaaaa cacgccgcag
     8701 ttgtgggctt ttacgccgag gctttgcagc aggctgtcgg cgtaaggttc ttgcccgtct
     8761 ggcttcgtta cgccctcgtt aaagctgcca aagcctgctg aaacgttgat tgttctgaag
     8821 ctgttaatca ttgaaacaat atcatcagcc attttttcta gtcgctcaaa ctctcttgcc
     8881 acaatactgg tattctctga ttgtggtttg ctttccttac ctgttgccag ccattcaagg
     8941 ctcacatttg acacctctgc tatttttaca agatttgaac gagatggatc tgcctctcct
     9001 tttacccacc tagatagtga tgcttgggct acccccactg ctcttgcaaa ctcactgtaa
     9061 ttgtccttaa agctattttt tgcaatccat ttcattcttt ctgagaagct ctcatcgtaa
     9121 atgtttggtt tactcatctt ttacgtttcc attaataaag ttttactcgc aaaacatact
     9181 cataaaccat tgatttttaa gttatttatg aataaagcaa aaatatttta gatgttttta
     9241 tgttttacgg ttgatttgtt taaagtttta cggttaaata cacgcatcaa atagataaaa
     9301 agaggtgtat cgaatgagtg tattaaacga tgttaaaaaa accgcacaga gagattggca
     9361 taaagccgat attcttgcgg aacttcgtaa aaaaggttgg tcacttcgtt ctttggctaa
     9421 agcaggcaat gttagccata acacgctgaa gtctgtttta gacaagcctt accccaaaat
     9481 ggaacgctta gttgcaaatg ccataggggt tgcccctgaa gtgatttggg ctgggcgaac
     9541 agcagaacgc attgaacgta atcggcgacc aactttaact aataagtttt aatcataaat
     9601 gatctttaac gtaaaagata taggagaaga acatttatga gcgaattaag tttaaaaacg
     9661 cactattcag tggctgaatt gttaagtttt aagttatcta gcctaccaag tgctcataaa
     9721 aatgtgcttg agaaagcaat aagggaaaac tggcaatctc aaaaacgcaa aggcagaggc
     9781 ggcggggttg aatatgaatt aatcagttta cccaccgaag tccaacagga aatccgcacc
     9841 aagttactca aacttcttcc tgctgaaatc agcaaaggcg aattgagtgt agtgcgacaa
     9901 aacattgatt tggagcagat aaccgataaa caactttcta cggcagatgc tcgcattatg
     9961 gtggtgcggt ggttcttgat gcaggaagtg cagttggggc taagccgcac aaaaacgcta
    10021 gaccaagtca ttgcagcggt ggcaagcagt gaaattcctg ctgaaatttg caaagcgatt
    10081 atggcgggca atagtaaagc cggtggaaag ttaaagctat ctaaacgcac cttgcatagt
    10141 tgggtgctgg cgtatgaggc aggcgaaaat tcagcggaac ggttaaaaca gatgataccg
    10201 ctgaaaacac aaaaacgtgc cgtgcccgag cggtgtggtt ggttgcaagc ctttctgccg
    10261 ttttatcaaa cctttagcaa tgtggcactc acacaggctt atgctcaatt tgcaatgcag
    10321 tatgagggcg aagatttacc tacagagagc caagttcggt atgccttgaa gcagttgccc
    10381 gattatgtgg tgcaacaagg gcggaaattg ccggtgaaga agatccggct cgcccgttaa
    10441 atacccttga agttaaaggc ttaacggtcg tagatgactc gcagaaaccg ttatttagcg
    10501 aagtaaacca agcgttatat cacggtttaa gcccgattga ggtggtggca agccgtgtgc
    10561 agattacccg tgcgattact acatacacca aaaacgtgac gaatactgat gacccaagct
    10621 atttggattt aaccaccatt cgtacgcttg actatgtgcg taaggcaatc caaacccgtc
    10681 agcgtttgcg tttccctcgt gcgaaaaact cccaccgtat tgtggcaaaa gtacgctcgg
    10741 agattttaga tgtgctttat cagcttgagg gcgaagaggt aatcgaaaat gtggaagcgt
    10801 ggaaaaatcg cttattagtg gtgcgtaatc aagacccgac ttacttggat ttagaaatcc
    10861 ctgcggatgt ggttaatggc ttgcacgtta ttcgtaacaa aattacgtta attttatagg
    10921 gggtgctaga tgtcaattgt ttatgaaggc tcttgcgtgc ttgaggtaga tggcgttgag
    10981 attgaaatta ccaaacttga tgtaaaaacc gtaaccggtc gcaagctggt taaaacgatg
    11041 aacagttccg gacgtgcaag aggttattcg caaggcattg cggaatatac tctctcggtg
    11101 actgcagttg agcctcaaga cggttcggta attgattggg ataacattaa aaatgcgaag
    11161 ctcactaaat acccgttaga caatagcgaa aaacgcacct cttatttagg ctgttttact
    11221 accgatgtga gcgagtctta caccgttgat ggtgagtcgg tgcgtgatat tcaactcaac
    11281 gcattgcgta aggtgaatga ataatgaaaa tcactttaaa tcttggcatt gaatataacg
    11341 ggaaaattcg ccgtgattgt acggtgcgat tgctgacgat gggcggtcaa tgcacggctc
    11401 aagaaatgat tgcggattta gggtttgagg aaaactcgga agccctatca aagcgtgaac
    11461 aaaccttggt cgatatggct tacctttcgc agcaagttac ctttgatggt atttatcaag
    11521 gtaatgtgac ggctcaattt ttatttgaca atttagctac cgaagactac tggcagttga
    11581 ttgaggcaac gctcacactc cgaaaaaagc ggttagaaaa tggggtgagc caaagcgaga
    11641 gcgaaagccc ggcaaatccc gatttggcgt agcagaagcc tttaagcagt accgacaagc
    11701 ggtgatttta ctctcaaaat ttgcaattac cgccgagcag gtatggcaaa tgccgttgct
    11761 tgaggtattt gcgtggattg agagctactt ggagagtgag ggcgtaaaaa caccgcatag
    11821 cccaatgggt gcgaatagcg aagtgaaacg cgaaagctat gtctttactc ggtgtggcaa
    11881 aattgaatag gggcgaaagc cccttttttg tactttaaaa ttagtttaaa gagactttaa
    11941 aaatggcaaa aaatatgaaa gtgcaactgg aattgacggc aaaagatact gcctccccag
    12001 ttttaaacca agccgctaaa accgccgaaa aagcctttaa aaatacagag cgagcggcac
    12061 aaaaaagtgg caatgctcaa acgcaagcag cacagaaagc agcgaatgct acagaaagct
    12121 caaatcggaa aatagaacag agttaccgta atgcccgccg agtggctgcc gatgtgagcc
    12181 gagcccgtga aacattaggt gttcgggcgg aaaatgcaat ccaacgcgaa attgaacaaa
    12241 ctcgtgcagc ctatgaccgc ttaaaacgct caggtgttgc ctctcaaaat gagctacgcc
    12301 gtgcctcaga agccactaag caacgggtgc gagagctgaa tgcagaattg ggtaagacaa
    12361 gctttgggga taaggctaaa ggatttgggc gtggtttgat gagtgccggt gtcggtattg
    12421 cagcaggtgc tgcaatggct cgtcagccag ccaagaacca aatggagttt gaccgacagt
    12481 tagcgatggc atcaaacact gccttttccg accgtgatgt taaagggcgt attgagggca
    12541 agaaagagtt attagcagcg gtgcaagagg cggttaaggt tgggggtgga acaaaagaag
    12601 atgctctttt aactcttgat acgctaattg cctcaggaac gatgaaggct gaaactgcaa
    12661 tgagcctatt accaaccttg cagaaaggtg ctgtagcaac aggtgcaagc tccgaggata
    12721 tggcaaaaat tgcgatctcc tcaatgcaac aatttgggat taaagaagag gatattgggc
    12781 gggcattgga tatggcagtt gcagccgggc aagcaggtaa ctttgagctt gcagatatgg
    12841 ctcgctggct accacaacaa atggccgcag gtaacacggc aaggcattgg gcggtttatt
    12901 accgcctgta tcgtatgacc caaacggcag atggctagcg ttatcccttg aagttgaggg
    12961 gagagagcta gaccgcattg atgcgagagc caagacttta atcgatgccg ttgatgtatc
    13021 aacaggcatt tttattgatg attgggagcg ggtttgtggc ttaacggcga atagctcact
    13081 accgcttgaa acccgaattg agcgtgtgat tgcaaagctg aatcagttgg gtggattgtc
    13141 gataggctat atcactcaag tggccaaaga gcttggatat tgcatcaaaa ttagtaagcc
    13201 acaacccttt attgctgggc taagccgagc tggggatatg ttatggcata aagacattat
    13261 gtggacattt tttgtggatg tttgcaaccc cacagatgat tacaagcgtt ttagagcaag
    13321 cgtgtcaatg gctggcgatg cgttaatggg acgatacaaa gacccaattt tagaaagtct
    13381 acttgaagag gtaaaacctg catttagccg tgtgtgggta agatatttag gagatattga
    13441 atgaaacaag tgatgaatcc gattaatacc ccaacacagc gttttaaaga tggcaaccct
    13501 gccactggtg aatatggcac gattgtgact gctgaatttt tgaataacgt ccaagattcg
    13561 gtcattaaca cccaacaaga attgcacagt gtacttgctg aagcaggaat cgaagcaaat
    13621 gacgaacaag ttaatcaggt ggctaaagca atcaagaaaa ttgcgggcga tgctactcgt
    13681 gataatttta acgcattagc caaccctgat ggttacaaac acatcggtcg ctgcaaatcg
    13741 gttgcggagt tacgtactat ccgcccaacg gaacacggtc agcgtatttt ggtggatgct
    13801 tactatgagg acggcacaac aggcggtggg gagtttgtgg ctgatttgca ggatttagtt
    13861 acgcctgatg atggtggggt ttgttttgtt gtgaataata atggggggcg gtggaagcgt
    13921 gttgaccttt cacatcttac attatttgat tttggggccg tcggtgacgg ggtaacaaat
    13981 gatgaatcag catttgtaaa tgctatgaga tattctcaat tttttatcga aaacggtaca
    14041 tttcgtatca acaatgccgt taattctgtt cgcgataatg tgcaaatttt gggcaataag
    14101 accggcaaat tagtattagg tgcaggtatc cagcaagcag aggccgaagt ttttaatatt
    14161 aatcacagca attattttat ctcgggtttt tgtattgaaa caccgaacaa agcaattggt
    14221 attcgattta agtctttgga tgatgctggt gttaaaaatc tccatattga caatgttgtg
    14281 tttaatggca ccttctacgg ggtcagggca ggtgaaagta ttcaggcgga tactaattac
    14341 ccaactgata atgtgattgt acaaaactgc caatcgtatt gtggttcagg taatgctgga
    14401 cattatctct gcaccaaagt taaaggtgtg cgatttttta acaatatagc aattggaggt
    14461 cgcaatgtat cagcttacgg tgcaacatct tgctctgata ttttcatttt gggtaatcgt
    14521 gaacagggga tggcagtgac gacagttgat gtggaagcgg gcgtacaagt ggaagatgta
    14581 caaactggcg aatgtgtggt agcgaataat atttgcagcc acgatatttg gatgtctggt
    14641 gctaaaaatg ccattatcaa cagcaacctc tgccgtcgct tgcgtgtcac atcaggtaat
    14701 ccgactgaca caggcagcaa caatgtagaa ttttcaaata atcaagcaaa atctattgca
    14761 atatcgaaat atggttcttt cgataatcct tcattgttta ttgataatat caattttatt
    14821 aataacattt tggagcccag tgaatttaat caagatgtgt atattgacgg gacaatttta
    14881 aaacagtgcc aatttattaa taatcgttgt attagcaagt ctaagcaata ttctgtcaga
    14941 cttgctcgca ataatccgtg cttaaattta acatttaaga gtaataaatt atcggacaat
    15001 cctgcgatta tatcaggtaa gcaaggtgtt attttctgcg atgatttgag cttaggagta
    15061 aataagcaca gattaagatt gcgattttca tcgccgatta attttactgt taatcaagaa
    15121 tggcaaaaaa tgccgtttga cagagtgtta aatgatatca atggtcatgt tgatattgtc
    15181 agtggtcaat ttattgcaag gacaggcggc acttttcatt taaaaggtaa aataacggtt
    15241 acaaattcat cggaagatgt gctgaattta tatcaactca ggctgttaat aaataacgaa
    15301 gagaaagaaa gactgattaa tcaaagtgtt tcacctaaaa acagcaaatc aaatacaaat
    15361 ggtatgacaa caatcaattt tgatactgta gtttatttaa aagtaggtga cattgttgat
    15421 ttgcagtata aaacaacgca tgagttgaca ttactgtcag actcattttt atctcaaatt
    15481 tatgtgactc aggagtttta agtgactaaa aaacactact gcacaggctg gcgtaatgcg
    15541 ccagccaata tcaatagttg ctgtcatcag catgaccgag actacggtat caagggcaca
    15601 gtcagccgag ccgaagccga caagcggtta agacattgct taatttcgca agggaatccg
    15661 attaaggcgt gggtgttttg ggttgtggtg aggttgtttg ggtggattta ttacaaggag
    15721 aaaaatatca atggaaagtc ataaaacagg aaatatcatt act
//
LOCUS       DANLZC010000048        15738 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.048, whole genome shotgun sequence.
ACCESSION   DANLZC010000048 DANLZC010000000
VERSION     DANLZC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15738
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.048"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..750)
                     /gene="dusB"
                     /locus_tag="PXE44_001155"
     CDS             complement(<1..750)
                     /gene="dusB"
                     /locus_tag="PXE44_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL5588672.1"
                     /translation="MQIGQYAIKNRIFLAPMAGITDQPFRRLCSQLGAGLTFSEMMST
                     NPDVWHTEKSRLRLAHHDEIGLNAVQIAGSDPVEMAKAAKINVEYGADIIDINMGCPA
                     KKVNKNMAGSALLRNPKLVAQILDSVVNAVDVPVTLKIRTGWDQENRNCLQIAKIAEQ
                     AGIKALTIHGRTRSCLFNGEAEYESIKAVKEAISIPVIANGDIDSAEKAKFVLDYTKA
                     DAIMIGRASFGNPWIFKEINDFFETGLSSPLS"
     gene            complement(974..1462)
                     /gene="sixA"
                     /locus_tag="PXE44_001156"
     CDS             complement(974..1462)
                     /gene="sixA"
                     /locus_tag="PXE44_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDL5588673.1"
                     /translation="MNIWIMRHGEAGFNASSDAARCLTDNGIKNTISQGKWLGEHLAN
                     QQIQLDKILVSPYLRAQQTLENVLIGMQAVNFPQHFTNLIESWDEVTPDGNPHTVESY
                     LHFLREEGAKNILLISHLPLVFDLVQILTNNQSNVQFPTSTIAEINWVKTKAELIRVK
                     HT"
     gene            1589..3028
                     /gene="pyk"
                     /locus_tag="PXE44_001157"
     CDS             1589..3028
                     /gene="pyk"
                     /locus_tag="PXE44_001157"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL5588674.1"
                     /translation="MSRKLRRTKIVCTMGPATDRGNTLEKIIAAGANMVRMNFSHGIP
                     EDHIERANKVREIAARLGRHVAILGDLQGPKIRVSTFKDGKIFLNIGDKFTLDADLPR
                     GEGHQEAVGLDYKNLPNDVVPGDILLLDDGNVQLKVLEVEGVKVHTEVTVGGPLSNNK
                     GINKLGGGLSAPALTDKDKEDIKLAAKIGVDYLAVSFPQSSADLHYARELAKEAGLEA
                     KIVAKVERAETVATEEAMDDIILGADVVMVARGDLGVEIGDAALVGVQKRLIRRARKL
                     NRVVITATQMMESMIKKPMPTRAEVMDVANAVLDGTDAVMLSGETANGDYPVETVKSM
                     AEVCLGAETMPSINISRHRMEGTFKSIEEAVAMSAMYTANHLEGVSAIISLTHSGETA
                     KLTSRISSGLPIYAMSRNQKALNLCALYRGVTPVYYNEDSRTMEGAKKAIAQLKEQGY
                     LLTGELVLLTHGDELKAGGTNTCRILRVE"
     gene            3124..4161
                     /locus_tag="PXE44_001158"
     CDS             3124..4161
                     /locus_tag="PXE44_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5588675.1"
                     /translation="MHNKLLSICILRLSAIGDVCHTLAVIQAIQRKYPSAEITWIIGK
                     TEAYLMKDIPNVHLIPYDKKIGWKGIFTLWQKLSHKRFDFLLNLQTAFRASILSLGIK
                     AKKKIGFNQDRAREMQWLFTNQKVEMTDSPHVLDGQMMFAKAIGIMDLSAQWNLPVSD
                     EDLAYGKQFIDFTRKNILIAPCSSKKEKDWLPERYAEIANWLSKQNINVLIAGSPSQY
                     EMETAAKIQQLAPNCTSIAGKTTLKQLAGVIRQVDLVISSDSGPAHIATTQNTPVIGL
                     YAIHNPRRTGPYNDLDKVVSVYDEAILQSYGKPWQQLAWATKAKGKNWMEKITVESVK
                     QKVVETLKITL"
     gene            4201..5259
                     /locus_tag="PXE44_001159"
     CDS             4201..5259
                     /locus_tag="PXE44_001159"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL5588676.1"
                     /translation="MNTVFNQDSVHNVNIQDEKVLLTPQELKQEFPLPEHLRNQIERS
                     RKTISDIIHRRDKRQLIVIGPCSIHDPVAALEYGKKLKALSEQVSDKLYIVMRVYFEK
                     PRTTVGWKGLINDPKMDGSFDVETGLRIARKLCLDLAELGLPLATEALDPMTPQYLAD
                     LFSWSAIGARTTESQTHRELASGLSMAVGFKNGTDGDLAVALNAMQSSAQSHSFIGIN
                     QQGQVTLLKTKGNADGHIILRGGKTPNYEKQYVDDCEWVLKQANLPTAIMIDCSHGNS
                     NKDYRRQPLVAENVLEQLLAGNTSIIGLMIESNLHAGNQSSELPFEQMKYGVSITDAC
                     IDWQTTEELLTHFAEKLR"
     gene            5314..6435
                     /gene="tyrA"
                     /locus_tag="PXE44_001160"
     CDS             5314..6435
                     /gene="tyrA"
                     /locus_tag="PXE44_001160"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDL5588677.1"
                     /translation="MNPLAPLREQIDQVDQQLIALLSERLALVAEVGKVKSEHGIPVY
                     APERETAMISARRSEAEKQGIPADLIEDVLRRIMRESYANENKHGFKRVNSDIQKIVI
                     VGGKGKLGGLFGNYLTLSGYNVEALGRDDWDNAEQILANADVVIVCVPIANTLATIER
                     LQSYLTENMILADLTSVKHQPLQKMLEVHNGAVVGLHPMFGPDIASQAKQVVACCHGR
                     FPERYQWLLEQIKIWGAKIEEIEAAAHDNAMTYIQALRHFSTFAFGLHLSRQPVQLSQ
                     LLALSSPIYRLELAMIGRLFAQDGSLYADIIADKPENLATIETLKESFEQGLDFFKRN
                     DKAGFIKAFEEVHHWFGDYSEQFLKESRVLLQQAHDSRK"
     gene            complement(6626..7711)
                     /gene="recF"
                     /locus_tag="PXE44_001161"
     CDS             complement(6626..7711)
                     /gene="recF"
                     /locus_tag="PXE44_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL5588678.1"
                     /translation="MALTRLLINHFRNIQHTDLAFSPHFNFLVGANGSGKTSLLESIF
                     YLGHGRSFKSHISNRIIHYDKDDFVLHGKIDEAKHSWSVGIQKFRSGETTLKINGEDG
                     NKIADLAHLLPMQVITPEGLTLLNGGPSFRRAFLDWGLFHQHPDFYAHWNNLRRLLKQ
                     RNSALQQVRSYQELKAWDIELVKTTYAVSEMRAEYAEALRPEIEKTCQFFLPEIEIGL
                     SFHQGWEKGADYAEILAQGFERDKALGYTMIGAQKADFRFKANGLPVEDVLSRGQLKL
                     LMCALRLAQGEHLVAQKQRQCLFLIDDFASELDPTKRELLAHRLRESGSQVFVTAITQ
                     DQLNQMQWQDRSDDCLFTVKNGEIQPQ"
     gene            7851..8180
                     /locus_tag="PXE44_001162"
     CDS             7851..8180
                     /locus_tag="PXE44_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL5588679.1"
                     /translation="MFGKGGLGGLMKQAQQMQERMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRIEIDPSLMEDDKEMVEDLVAAAFNDAVRRAEEMQKEKMASVTAGMQLP
                     PGMKFPF"
     gene            8188..8793
                     /gene="recR"
                     /locus_tag="PXE44_001163"
     CDS             8188..8793
                     /gene="recR"
                     /locus_tag="PXE44_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL5588680.1"
                     /translation="MQTSPLLENLMEALRALPGVGPKSAQRMAYHLLQRNRSGGVALS
                     KALNEAMSHIGHCKSCRTFTEEEECTICKNPRRQMSGQLCVVEMPEDIQAIEQTGQFS
                     GRYFVLMGHLSPIDGIGPREIGLDLLQQRLEQESFHEVILATNPTIEGDATANYIAEM
                     CRMYNVKATRIAHGVPVGGSLEMVDGTTLSHSFAGRRDIDI"
     gene            8865..9191
                     /gene="grxD"
                     /locus_tag="PXE44_001164"
     CDS             8865..9191
                     /gene="grxD"
                     /locus_tag="PXE44_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HDL5588681.1"
                     /translation="METIDKIKQQISENPILLYMKGSPKFPSCGFSARAVEAVINCQV
                     PFGYVDILTNPDIRAELPKFANWPTFPQLWVEGELVGGCDIVLEMLQKGELQTLLKET
                     AAKHAA"
     gene            9333..10370
                     /locus_tag="PXE44_001165"
     CDS             9333..10370
                     /locus_tag="PXE44_001165"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL5588682.1"
                     /translation="MALKELDWANLGFSYIKTDYRFIAHWKDGKWNEGELTQDNTLHI
                     HEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAERMQRTADRLLMPRVPTELFIR
                     ACKKVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKAAPEFIFSVFCCPVGAYFKG
                     GLAPSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELAVEQSSTTRKFADAIYLDPK
                     THTKIEEVGAANFFGITKDNKFITPISESILPSITKYSLLHLAQERLGMEAIEGDVYI
                     DQLDQFAEAGACGTAAVITPVGGIQHKDKFHVFYSETEVGPVTRRLYAELTGIQFGDV
                     EAPQGWIVKVE"
     gene            10397..10945
                     /locus_tag="PXE44_001166"
     CDS             10397..10945
                     /locus_tag="PXE44_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HDL5588683.1"
                     /translation="MAKLFANSTACLYLENMKKLIFLGLSALALAGCSSTISNPNQLI
                     GQWQCTIEYDDFNIRTVDNLRFSTNGTMTNQGGIYYPIQKSIFEYSLQQNGRWSVKNG
                     AIVYRIVSESVQRSHRSQIWSELQRDAELQQFEENLFSSLSDSDNNKTVELIITDFDE
                     KEMDIKQEIKGHKAYKGQCIKQ"
     gene            10954..11298
                     /locus_tag="PXE44_001167"
     CDS             10954..11298
                     /locus_tag="PXE44_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL5588684.1"
                     /translation="MIQRFEVGSRFSEMAIHNGVAYLAGQVPLDDTADAYTQTQQVLA
                     EIDKWLAKANSDKSKILMATVYLKEMADYAEMNRAWDEWVAPNNAPPRAAVEAKLANP
                     NWKVEIVVTAAV"
     gene            complement(11331..13109)
                     /locus_tag="PXE44_001168"
     CDS             complement(11331..13109)
                     /locus_tag="PXE44_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HDL5588685.1"
                     /translation="MSTLDFHFIHRFHQQVQALSHHPALRFMQDEKWSEISWLELQKQ
                     VNNLSYALLANGLAVQEKIAIFAQNMPRWTITDIAAMQIRAVAVPIYATSTTKQLEYI
                     LNDGDIKILFVGDQEQYDQAIEIANNCSLLIKIVAMKDSIDLRDFPIAQHWQDFIKVN
                     QDEAELTKRLNDKCLEDLFTLIYTSGTTGEPKGVMLDYANLAHQLQAHNKALPHIDEN
                     DISLSFLPLSHIFERAWVTYVLYRGAVNCYLEDPQQVRQALSTVRPTLMCAVPRFYEK
                     IYTAVWDKVQQAPIHRRALFKWAIRTAEDNLFGKKSAFFHRLQYIMADKLVLGKLRAL
                     LGGRIRMMPCGGAKLDPSIGLFFQSIGINVKLGYGMTETTATISCWQEKGFNINSTGT
                     LMPDVEVKISEENEILVRGGLVMKGYYKKPEATAQAFTAEGFLKTGDAGELDSEGNLY
                     ITDRIKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEE
                     YAKKLNIRYQDRLELIKHYEVVRMFEQRINELQKDLASFEQIKKFTLLSQAFSTKMEE
                     ITPTLKLRRKVILERYKQQIEKMYES"
     gene            complement(13335..13904)
                     /locus_tag="PXE44_001169"
     CDS             complement(13335..13904)
                     /locus_tag="PXE44_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDL5588686.1"
                     /translation="MNQENHKIEDIIRIFNQCFSDEYNTRLERGGNYPIYLPEFMDED
                     GVPSERPYNVILFAHGYYSSALHEIAHWLVAGEARRKLEDFGYWYEPDGRSAERQREF
                     ESAEIKPQAIEWVLATAAGFRYFASADNLSGNPGDNSAFKQAVYDQVKLYAERGYLPK
                     RAETLRKALCAFYGTPDVIDLAQFDIERI"
     gene            complement(13914..15677)
                     /locus_tag="PXE44_001170"
     CDS             complement(13914..15677)
                     /locus_tag="PXE44_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDL5588687.1"
                     /translation="MIRYFFSLVLSLLMINSSHANLFSQPKFLKSEEAFVFSIQNENE
                     TLQLNWDIADNYYLYKKEIRVTPQNIEIGDITFPAAEQYNDEFFGQVEIYRNHLGLKV
                     PFKEIKDNASIVVEYQGCTKGFCYPPERVELKLDSILSATPEELAQLEAQAVEKSENF
                     AKTDNMAPPENVAKAEQDQLAENLANNRFSIFWFFVLGVGLAFTPCVLPMLPLLSAIV
                     IGNKQRPSTAKALLLSIAYVQGMAVTYTLLGLIVAAIGLPFQIALQSPPVLIGLSIVF
                     ILLACSMFGLYEIQLPNSWQQKLNAMSQKQQGGALGSVFLMGMIAGLIASPCTSAPLS
                     GALLYVAQSGDLVTGGLALYLLALGMGLPLILITLFGNKILPKSGEWLLKVKAAFGFV
                     MLALPVFLLSRVLPSHYEPFLWSALAVAFLIWLVDAIPSNSITKKIIKVLLLLALAIA
                     AKPWADLAWNGSTTQQQTTPHLALEKIQSLADLETKLTASKGKKVMLDLYADWCVACK
                     EFEKYTFSDQNVQNKLNEMVVLQIDMTKNSAENIELMKHFNVLGLPTILFFDENGNEM
                     SQSRVTGFLDAEQFLAWLNKL"
BASE COUNT         4731 a         3034 c         3368 g         4605 t
ORIGIN      
        1 tgataatggt gaagataatc cagtttcaaa aaaatcatta atttccttaa atatccaagg
       61 atttccaaag ctagcgcgcc caatcatgat tgcatcggct ttggtatagt cgagaacaaa
      121 ctttgctttt tccgcagagt caatgtctcc gtttgctatc actgggatag aaattgcttc
      181 ttttactgcc ttaatacttt catattcagc ttcgccatta aataaacagc ttcgagtacg
      241 cccgtgaata gtgagagctt ttatgccggc ttgttctgca attttcgcaa tttgcagaca
      301 atttctattt tcctgatccc aacctgttct aatttttagt gtcacaggga catcaacggc
      361 attgactaca gaatctaata tttgagcgac taatttgggg ttacgtaaaa gagcagagcc
      421 tgccatattt ttattcacct tttttgccgg gcaacccata ttaatatcaa taatatcggc
      481 accatactca acatttatct ttgcagcctt tgccatttca acaggatcag aacctgcaat
      541 ttgtacggca tttaagccta tttcatcgtg gtgtgctaac cgcaatcgtg atttctcagt
      601 atgccataca tcaggattgg tagacatcat ctctgaaaat gttaaacctg ctccaagttg
      661 gctacataag cggcgaaaag gctgatctgt gattccagcc ataggtgcaa ggaaaatacg
      721 atttttgatt gcatattgtc ctatttgcat ttttaacctt ttattaacaa atttgaagta
      781 attttaacca gagggatatc caaataaaaa gtacggtaaa taccgtactt tcttataata
      841 gcttatggat cagtttagta ctgatttgag ggctggtatg atacgcattt tttaggtatt
      901 ttggaagtct ataaattcga caatttataa aaaaattgaa aatttttata ttgacattct
      961 aaaaatgaaa gaattaagta tgctttactc gaattagctc agcttttgtc tttacccaat
     1021 taatttctgc tatggttgaa gttggaaatt gtacattgct ttggttattc gttaaaattt
     1081 ggacgagatc gaacacaagt gggagatgtg aaatcagaag aatatttttt gcgccttcct
     1141 ctcttaaaaa atgtagatag ctttctactg tatgcgggtt tccgtcaggc gtaacctcgt
     1201 cccaactttc aatgaggttg gtaaaatgtt ggggaaaatt gaccgcttgc ataccaataa
     1261 gcacattttc aagcgtttgt tgcgccctaa ggtatgggct gacgagaatt ttatctaatt
     1321 gaatttgttg gttggctaaa tgttctccta accattttcc ctgtgaaatg gtgtttttta
     1381 taccattgtc ggttaaacag cgagcagcat cactcgaagc attaaagcct gcttccccat
     1441 ggcgcataat ccaaatattc ataacattct cctttagttg tatttaaact ctcttgattt
     1501 aggcgtataa tgaccctcgt ttaacgcata atcaagatgc ttagaaagat tttaacttaa
     1561 tttttaaatt tattgatgga gtcaaataat gtctagaaaa ctcagaagaa cgaagatcgt
     1621 ttgtacaatg ggacctgcaa cagatcgtgg caatacgttg gaaaaaatca ttgctgccgg
     1681 tgcaaatatg gttcgtatga atttctctca cggtattcct gaagatcaca tcgaacgtgc
     1741 gaataaagtg cgtgaaattg ctgcaagatt aggtcgccac gttgcgattt taggcgattt
     1801 gcaaggtcct aaaattcgtg tttcgacatt taaagacggt aaaattttct taaatattgg
     1861 tgataaattc accttagacg cagatttacc gcgtggagaa ggccatcaag aagctgtagg
     1921 tttagattac aaaaacttac caaatgatgt agtacctggt gatattctat tgttagatga
     1981 cggtaatgtt caactgaaag tgttggaagt ggaaggcgtt aaagttcata ctgaagtgac
     2041 agttggtggc ccgttatcaa acaataaagg gattaacaaa ttaggtggtg gtttatcagc
     2101 tcctgcatta accgacaaag ataaagaaga cattaaatta gcggcgaaaa tcggtgtgga
     2161 ttacctagcg gtttcattcc ctcaatcaag tgcagattta cattatgcac gtgaattagc
     2221 aaaagaagca ggcttagaag cgaaaattgt agcgaaggtt gaacgtgccg aaactgttgc
     2281 gactgaagaa gcaatggacg acatcatttt aggtgcggac gttgtgatgg ttgctcgtgg
     2341 tgacttaggg gttgaaattg gtgatgcggc tttagtgggt gtgcaaaaac gtttaatccg
     2401 ccgtgctcgt aagctaaatc gtgtggtaat taccgcaacc caaatgatgg aatcgatgat
     2461 taaaaaacca atgccgaccc gtgctgaagt gatggatgtt gcaaatgctg tgttagatgg
     2521 tactgatgcg gtaatgcttt ccggcgaaac tgcaaacggt gactaccctg ttgaaacggt
     2581 aaaatcaatg gcagaagtgt gcttaggtgc tgaaacaatg ccaagcatta acatttctcg
     2641 ccaccgtatg gaaggtacgt ttaaatctat cgaagaagct gtggcaatgt ctgcaatgta
     2701 caccgcaaac catttagaag gtgtatcggc aattatttca ttaactcatt caggcgaaac
     2761 ggctaaatta accagccgta tcagctcagg tttaccgatt tatgcgatgt ctcgtaacca
     2821 aaaagcatta aacctttgtg cgttatatcg tggtgtaaca ccggtatatt acaatgaaga
     2881 cagccgcaca atggaagggg cgaaaaaagc cattgctcaa ttaaaagaac aaggctattt
     2941 attaaccggc gaattagtgc ttttaactca cggagatgaa ttaaaagctg gcggtacaaa
     3001 tacttgtcgt atcttaagag ttgaataatc taaacgatta acgcaaaagc cacataaaac
     3061 actttatgtg gctttttttc tgtcttaata tagcttaatg taatctgaac tattgatatt
     3121 attatgcaca ataaacttct ttctatctgt attctccgcc tttctgccat tggcgacgtt
     3181 tgccatacct tagcggttat tcaagctatt caacgaaaat atcctagtgc tgaaattact
     3241 tggattatcg gcaaaacgga agcgtattta atgaaagata ttccgaatgt tcacttaatt
     3301 ccttatgata aaaaaatagg gtggaaaggt atctttacac tttggcaaaa actttctcac
     3361 aagcggtttg attttttatt aaatttacaa acggcattcc gagcttcaat tctatcgctt
     3421 gggattaaag caaagaaaaa aatcggcttt aatcaagaca gagcaaggga aatgcaatgg
     3481 ttgtttacca accagaaagt agaaatgaca gactctcctc acgtgcttga cgggcaaatg
     3541 atgtttgcca aagccattgg gataatggat ttatcggcac aatggaattt acctgtgagt
     3601 gatgaagatt tagcttatgg caaacaattt attgatttta caaggaaaaa tatactcatt
     3661 gctccttgtt caagcaagaa agagaaagat tggttgccgg aacgctatgc agaaattgct
     3721 aactggctaa gtaagcaaaa tatcaatgta ttaattgcag gttctccatc gcaatatgaa
     3781 atggaaaccg cagccaaaat tcaacaactt gccccgaatt gtacgagtat tgccggtaaa
     3841 accacgctaa aacagcttgc aggtgttatt cgtcaggttg atttggtgat ctcttcagat
     3901 tcaggtcctg cccatatcgc cactacgcaa aatacgccgg tgattgggtt gtatgcgatt
     3961 cataatccaa gacggacagg cccttacaat gacttagaca aagtggtttc ggtatatgat
     4021 gaagcgattt tgcaatctta tggtaaacct tggcagcaat tagcttgggc aactaaagct
     4081 aaaggcaaaa attggatgga aaaaatcacg gtggagagtg tgaaacaaaa agtagttgaa
     4141 actttaaaaa ttacgttgta aagtttgtgt attaaaagat ttacacaaaa ggaaattaaa
     4201 atgaacacag tgtttaatca agatagcgtg cataacgtaa atatccaaga tgaaaaggta
     4261 ttactgaccc cacaagagtt aaaacaagaa tttccgttgc cggaacattt acgcaatcaa
     4321 attgaacgtt cacgcaaaac gatttccgat attattcaca gacgagataa acgccaactg
     4381 attgtgattg gaccttgttc gattcacgac ccagttgctg cccttgaata tggtaaaaaa
     4441 ttaaaagctc tttcggagca agtgagtgat aagttgtata ttgtgatgcg ggtttatttt
     4501 gaaaagcccc gcactactgt aggttggaaa gggctgatta acgatccgaa aatggacggc
     4561 tcttttgatg tcgaaaccgg cttacgtatc gcacgaaaac tctgtttgga tttagccgaa
     4621 cttggcttgc cgttagcgac cgaagcctta gatccgatga caccacaata tttagcggat
     4681 ttgttcagct ggtcggcaat tggagcaaga acaactgagt ctcaaactca ccgtgaatta
     4741 gcctcaggtt tgtcaatggc ggtaggcttt aaaaatggta cagatggtga tttagccgtt
     4801 gcacttaatg cgatgcaatc ttccgctcaa agccatagct ttatcggcat taaccaacag
     4861 gggcaagtta ctttactcaa aacaaaaggt aatgcagacg gacatattat tttacgtggc
     4921 ggtaaaaccc caaattatga aaagcaatat gttgatgatt gtgaatgggt gctaaaacaa
     4981 gcaaacttac ctacagcgat tatgattgat tgcagccacg gcaattccaa taaagattac
     5041 cgccgccaac cgctggtggc agaaaatgta ttagagcagc tacttgctgg caatacttcg
     5101 attatcggtt taatgattga aagcaattta cacgcaggta atcaatcatc agaattgccg
     5161 ttcgagcaga tgaaatacgg tgtttctatt acggatgctt gtattgactg gcaaactaca
     5221 gaagagcttt taactcactt tgctgaaaag ttgcgttaaa gataatttgc aaaaaattgc
     5281 ataaaaacga ccgcttgtaa attggtaaca aatatgaacc cattagcccc attacgagaa
     5341 caaattgatc aagttgatca acaacttatc gccttacttt ctgaacgctt agctcttgtg
     5401 gcagaagttg ggaaagtcaa atcagaacac ggcattcctg tttatgctcc ggagcgagaa
     5461 accgcaatga tttccgcccg ccgctctgaa gcggaaaaac aaggtattcc ggcggatctc
     5521 attgaagatg tgctacgccg tataatgcgt gaatcttatg ccaatgaaaa caaacacggc
     5581 ttcaaaagag ttaattcgga cattcaaaaa atcgtgattg ttggtggtaa agggaaatta
     5641 ggtgggctgt ttggcaatta tttaacccta tccggctata atgttgaggc attaggacgt
     5701 gatgattggg acaatgccga gcagatttta gccaatgcag atgtggttat tgtgtgtgtg
     5761 ccgattgcca acaccctagc aaccatcgag cgtttacaat cttatttaac cgaaaatatg
     5821 attttggcgg atttaacctc cgtcaaacat cagccgttgc aaaaaatgct ggaagtgcat
     5881 aatggagcgg tagtcggttt acacccgatg ttcggaccgg atattgcaag tcaggcaaag
     5941 caagtggtcg cttgctgcca cgggcgtttc cctgaacgtt atcaatggtt gcttgagcaa
     6001 atcaaaattt ggggggcaaa aattgaagag attgaggcag cagcacacga taatgcaatg
     6061 acctacattc aagccctacg ccacttttcc acttttgcat tcgggctaca tctttctcgc
     6121 cagccggtgc aactctctca attattggca ctatcttcgc cgatttaccg cttagagttg
     6181 gcaatgattg ggcgtttatt tgcccaagac ggcagcttat atgccgatat tattgccgat
     6241 aaaccggaaa atttagccac catcgaaacc ttaaaagaga gttttgaaca agggctagat
     6301 ttctttaaac gcaacgacaa agccggtttt atcaaggctt ttgaggaagt gcatcactgg
     6361 tttggcgatt attccgagca gttcttgaaa gagagtagag tattgctcca acaggctcac
     6421 gattcaagaa agtaaaggta ggttgaggtt ttgctctgat agcactagcg tccacgctag
     6481 tgctatttaa tattctacaa gcggttaata aagagctgtt ttttgcaaat ttttatgcaa
     6541 aattatccgc ttgttttggc acgagcgtgg acgctcgcgc catcggaaga aacatttttg
     6601 taaaattgcc cccaaatctg accgcttact gtggctgaat ttctccattt tttaccgtaa
     6661 acaaacaatc atcgcttctg tcttgccatt gcatttgatt gagctgatct tgagtaatgg
     6721 cggtaacgaa tacttgtgag ccactttctc ttaaacgatg tgctaaaagc tcacgtttgg
     6781 ttgggtcgag ttcggaggca aaatcgtcaa ttaaaaatag gcattggcgt tgtttttggg
     6841 cgaccaaatg ttcgccttga gccagtctta acgcacacat caatagcttt aattgcccac
     6901 gggaaagcac atcttccacc ggtaagccgt tcgctttaaa acggaaatcg gctttttgag
     6961 ctccaatcat cgtatagcca agagctttat cccgctcaaa accttgtgcc agaatctcgg
     7021 cataatccgc ccctttttcc cagccttgat gaaagctcaa gccgatttcg atttccggca
     7081 ggaaaaattg gcaggttttc tcaatttcgg ggcgtaacgc ttcggcgtat tcggctcgca
     7141 tttcgctcac ggcataagtg gttttcacca attcgatatc ccacgctttg agttcttgat
     7201 aagagcggac ttgttgcaaa gccgaattac gttgctttaa taaacggcga agattgttcc
     7261 aatgagcgta gaaatcaggg tgctggtgaa ataagcccca gtccaaaaaa gcacggcgaa
     7321 agctcggacc accgttaagt aaggttaagc cttcgggggt gatcacttgc atcggcaata
     7381 aatgggctaa atcggcaatt ttgttgccgt cttcaccgtt gattttaagt gtcgtctcgc
     7441 cactgcgaaa tttctgaatg ccgaccgacc aactgtgctt tgcctcgtca attttaccgt
     7501 gtagtacgaa atcgtcttta tcgtaatgga taatgcggtt gctgatatgg cttttgaatg
     7561 aacgcccgtg tccaagatag aaaattgatt ccaacaaact ggttttgcca cttccgttcg
     7621 ccccgactaa aaaattaaaa tgcgggctaa atgctaaatc ggtatgttgg atatttctaa
     7681 aatggttgat taataaacgg gttaaagcca tagttaaagt gcgaggaata aaaagagtag
     7741 ccattttagc aaatttgcaa aattttggga aaaattaacc gcttgcggat tagaaagtgg
     7801 ttcagtagaa taagccgttt attttttatt gaaaattaag aggaaaaatt atgtttggaa
     7861 aaggtggctt aggcggctta atgaaacagg ctcagcaaat gcaagagcgt atgcaaaaaa
     7921 tgcaagaaga gatcgcccaa ttagaagtaa caggcgagtc tggtgcgggt ttggtaaaag
     7981 tcactattaa cggggctcac aattgccgcc gaattgaaat tgatccatca ttaatggaag
     8041 atgataaaga gatggtggaa gatttagttg ccgctgcctt taacgatgcg gttcgccgtg
     8101 cagaagaaat gcaaaaagag aaaatggcaa gtgtgactgc cggtatgcaa ctgccgccgg
     8161 gtatgaaatt cccattctaa tttaagtatg caaaccagtc ctttacttga aaatttgatg
     8221 gaagccttgc gtgcattgcc cggtgtcgga ccgaaatcgg cacagcgaat ggcttaccat
     8281 ctgttgcaac gtaatcgttc cggcggtgtg gcgttgtcaa aagccttaaa tgaggcaatg
     8341 agccatatcg gacattgcaa atcgtgccgt acttttaccg aagaggaaga gtgtacgatt
     8401 tgtaaaaatc ctcgccgtca gatgagcggg caactttgtg tggtagaaat gccggaagac
     8461 attcaagcga ttgagcaaac cgggcaattt tcagggcgtt attttgtgct gatggggcat
     8521 ttgtcgccga ttgatggtat aggccctcgt gaaatcggct tagatttatt gcaacaacgc
     8581 ttggaacagg aatctttcca cgaagtgatt ttggcgacta atccgaccat tgaaggtgat
     8641 gctaccgcaa actacattgc tgaaatgtgc agaatgtata acgtgaaagc tactcgtatt
     8701 gctcacggtg tgccggtcgg tggttcatta gaaatggttg atggcacaac gctttctcac
     8761 tcttttgcag gacggcgtga tattgatatt taaagctatt tctgtgctaa cttaattctg
     8821 atataattac tcaactattt taaattatta ttttgaggct aaaaatggaa actattgaca
     8881 aaatcaaaca acaaatcagc gaaaatccga ttctacttta tatgaaaggt tcaccgaaat
     8941 tcccatcttg tggtttctcc gctcgtgcgg tggaagcggt aattaactgc caagtgcctt
     9001 tcggttatgt ggatatttta accaatccgg atattcgtgc ggaattgcca aaatttgcaa
     9061 actggccgac tttcccacaa ttatgggtag aaggtgaatt agttggcggt tgtgacatcg
     9121 tattagaaat gctccaaaaa ggcgaacttc aaaccttatt aaaagaaacc gcagcaaaac
     9181 acgcagcata atttcaaacc agtatgttaa ggctcttatg tttaagagcc ttaatttttt
     9241 cttgcgattt aggcttaaac ttcttacctc aagagctatt ttgttttaga atgtcaaaat
     9301 tatttcgtaa cataaaaata aaaggacaca acatggcatt aaaagagtta gattgggcaa
     9361 atttaggctt ttcttatatc aaaaccgatt accgtttcat tgcacattgg aaagacggaa
     9421 aatggaatga aggcgaatta actcaagaca atacactgca tattcacgaa ggctcaactg
     9481 ccctgcacta tgggcaacaa tgttttgagg ggttaaaagc ctatcgctgt aaagatggct
     9541 caattaactt attccgcccg gatcaaaatg ccgagcgtat gcaacgcacc gcagatcgtt
     9601 tattaatgcc acgtgtgcca accgagctgt ttattcgtgc ctgtaaaaaa gtggtaaaag
     9661 ccaatcaaga ttggttaggg ccttacggtt cgggggcaac cctttattta cgtccgtttt
     9721 taatcggcgt gggcgaaaat attggggtga aagctgcacc tgaatttatt ttttccgtat
     9781 tctgctgtcc ggtgggggca tatttcaaag gtggcttagc accctcaaac ttcattacaa
     9841 ccgactatga ccgtgcagcc ccaatgggaa caggtggtgt gaaagtaggc ggtaactacg
     9901 ctgcgagttt attaccgcac gaattggcgg ttgagcaaag ttcaactacc cgtaaatttg
     9961 ccgatgcgat ttatttagat ccgaaaaccc ataccaaaat tgaagaagtg ggggcggcga
    10021 acttctttgg gattaccaaa gacaacaaat tcatcacccc gatttcagag tcgattttgc
    10081 caagtattac caaatactca ttgctacacc ttgctcaaga gcgtttaggt atggaagcga
    10141 ttgagggaga tgtgtatatc gaccagttag accaatttgc cgaagccggt gcttgtggta
    10201 cggctgcggt gattacgccg gttggtggta ttcaacataa agacaaattc cacgtcttct
    10261 attcagaaac cgaagtgggg cctgtcaccc gccgtttata cgcagaatta accggtattc
    10321 aatttggcga tgttgaagca ccgcaaggct ggattgtaaa agtagagtaa ttttacacaa
    10381 gtaacaagcg gtcgaattgg caaaactttt tgcaaattca accgcttgtc tttatttaga
    10441 gaatatgaaa aagttaattt ttttaggttt atcagcactt gcattagctg gttgttcctc
    10501 aacaatttct aatcctaatc aactgattgg gcaatggcaa tgcactattg agtacgatga
    10561 ttttaatatt cgtacggtgg ataatttacg attttccaca aatgggacta tgaccaatca
    10621 aggtggtata tattacccta ttcaaaagtc gatttttgag tattcattac aacagaatgg
    10681 gcgctggagt gtgaaaaacg gggctattgt ttatcgtatc gtctcagaat ctgtacagcg
    10741 tagccatcgt tcacaaatat ggtctgaatt acaacgtgat gcggagctac aacaatttga
    10801 agagaattta tttagctcac tttctgatag tgataataat aaaacagtgg aactaatcat
    10861 cactgatttt gatgaaaaag aaatggatat caagcaagaa ataaaagggc ataaagctta
    10921 taaagggcaa tgtataaaac agtagaggta aaaatgattc aacgttttga agtaggtagc
    10981 cgtttttcgg aaatggcaat acataacggt gtggcttact tggcagggca agtgccgtta
    11041 gatgacaccg ccgatgctta cacccaaacc cagcaagtgc ttgcagaaat tgataaatgg
    11101 ctggcaaaag ccaacagcga taaaagtaaa attctaatgg ctacggttta tttaaaagag
    11161 atggcagatt atgcggaaat gaaccgtgcg tgggacgaat gggttgcccc aaataacgct
    11221 ccaccaagag cggcggtgga ggctaaatta gccaatccaa attggaaagt cgaaattgtg
    11281 gttaccgctg cggtataaat gaaaagcaga aagacgatct ctgtttttca ttatgattcg
    11341 tacatttttt cgatttgttg cttatagcgt tctagaatga cctttcgtct taattttaat
    11401 gtaggcgtaa tttcttccat tttggtgcta aatgcttgcg aaagtagtgt aaactttttg
    11461 atctgttcaa aacttgcaag gtctttttgt aattcgttaa tgcgttgttc aaacattctg
    11521 acaacctcat aatgtttaat gagttctaat ctgtcttgat agcgaatatt gagctttttt
    11581 gcatactctt cgaggctatc aaagcaagga actatcaacg cagaaacata ttttttggca
    11641 tcagcaatca ccgcaatttg ctcaataaac ttatctttac ctatttttcc ttcaatgtat
    11701 tgtggggcaa tatatttacc gtttgaagtt ttcattaatt ctttaatacg atcggtaata
    11761 taaagattgc cttccgaatc cagttcaccg gcatctcccg tttttagaaa accttcagca
    11821 gtaaaggctt gagcagtggc ttccggtttt ttgtagtaac ctttcataac caagcctcca
    11881 cgcactaaaa tttcattttc ttcactaatt ttgacctcaa catcaggcat taaggtaccg
    11941 gttgagttga tattaaagcc tttttcctgc cagcatgaaa tagtggcagt agtttcggtc
    12001 attccgtatc caagttttac attgatgcct atactttgga aaaatagccc gatagacgga
    12061 tctaatttag ctcctccaca cggcatcata cgaatacgac cgcctagtaa ggctcgtaat
    12121 tttcctagca ccaatttatc tgccataatg tactgcaagc ggtggaaaaa ggctgatttt
    12181 ttaccaaaca gattgtcttc ggcggtacgg attgcccatt taaataacgc tcggcgatgt
    12241 atcggtgctt gttggacttt atcccacacc gcagtataga ttttttcata gaaccttggt
    12301 acggcacaca ttaatgtagg tcgtaccgtt gagagtgctt gacgtacctg ttgaggatct
    12361 tccagatagc aatttactgc ccctctgtat agcacataag tgacccaagc acgttcaaag
    12421 atatgcgata aaggtaaaaa tgaaagggat atatcatttt cgtcaatatg agggagtgct
    12481 ttattatgtg cctgaagttg atgagctaaa ttagcataat ctaacataac acctttaggc
    12541 tcaccggttg ttcccgaggt ataaattaaa gtgaataaat cctctaagca tttatcattt
    12601 aaacgtttgg ttaattcagc ttcatcttga ttaaccttaa tgaaatcttg ccaatgttga
    12661 gcgataggga aatcacgcag atctattgag tctttcatcg cgacaatttt tattaatagc
    12721 gaacagttgt tcgctatttc tatggcttga tcatattgtt cctgatcgcc aacaaagagg
    12781 atttttatat ccccgtcatt caggatatat tcaagttgtt tggtggtact ggttgcataa
    12841 atcggcactg ctaccgcacg aatttgcatt gcagcaatat cggtaattgt ccaacgaggc
    12901 atattttgtg caaaaatagc gattttctct tgaacggcaa gaccgtttgc cagcaaggca
    12961 taagaaagat tattgacctg tttttgtaat tctaaccaac ttatttctga ccatttttca
    13021 tcttgcataa aacgtaaagc tgggtggtgg ctaagagcct gaacttgctg gtggaaacgg
    13081 tggataaagt gaaaatctaa agtgctcata atcttatttt tactttctat ttcagtgaac
    13141 agttgtacgc taatataata gaaaatatga aaaaaagaaa cagttgtact ctgaaacaaa
    13201 gggttttgga taggaagtaa tcaaggcaat agaaatagat aagtagggta tcgtttagta
    13261 caaatgcaat atttttcaaa aatgagaccg cttgtatatg caacaagcgg tctgttttgt
    13321 taaaaatttt gcaactaaat tcgttcaata tcaaattgag ctaaatcaat cacatcaggt
    13381 gtaccgtaaa aagcacagag ggctttacgc aaggtttcag ctcgtttggg aaggtagcca
    13441 cgttctgcat agagcttgac ttggtcgtac actgcttgtt taaaggctga attatcgccg
    13501 ggattgcccg agagattgtc ggcactagca aaataacgaa aaccggcagc agttgctaac
    13561 acccattcaa tcgcttgcgg tttgatttcc gcactttcaa attcacgttg gcgttcggca
    13621 gagcgtccgt ccggctcgta ccaatagcca aaatcttcaa gtttacgccg agcttctcca
    13681 gcaacgagcc aatgggcaat ttcgtgcaag gcactgctgt aatagccgtg agcaaataaa
    13741 atcacattgt aagggcgttc gctcggcaca ccatcttcgt ccataaattc gggcaagtaa
    13801 atcggataat tacccccccg ctctaggcga gtgttatatt catcagaaaa gcattgatta
    13861 aaaatgcgaa taatgtcttc aattttatga ttttcttgat tcatttcttt tctttataac
    13921 ttattcagcc aagctaaaaa ttgctcggca tccaaaaagc cagtaacacg agattggctc
    13981 atttcattgc cgttttcatc aaaaaacaga atcgttggca aacctaatac attaaagtgt
    14041 ttcatcagct cgatattttc agccgagttt ttcgtcatat cgatttgcaa cactaccatt
    14101 tcattcagtt tgttctgaac attttggtca ctaaaggtgt atttttcaaa ctctttgcag
    14161 gcgacacacc agtcggcata gagatcgagc atcacttttt tacctttgga agcggtcaat
    14221 tttgtctcta aatcggcaag tgattgaatt ttttctaacg ctaagtgcgg tgtagtttgc
    14281 tgttgagtgg ttgagccgtt ccaagctaaa tctgcccaag gtttagcggc aatggctaaa
    14341 gcgagcaata ataagacttt gatgatcttt ttcgtgattg aattagatgg aatcgcatcc
    14401 accaaccaga tcaaaaatgc aaccgcaagg gccgaccata aaaacggttc atagtggctt
    14461 ggcagcacac ggcttaataa gaacaccggt aaggcaagca taacaaaacc gaatgcagct
    14521 ttcactttta acagccattc gccggatttt ggcagaattt tgttgccgaa taaggtaatc
    14581 aaaatcagcg gtaagcccat tcccagtgcg agtagataga gggctaagcc accggtgact
    14641 aaatcgccac tttgtgccac ataaagcaac gcaccggaaa gtggggcaga agtacaaggg
    14701 gaagcaatta agcctgcgat catccccatt aaaaacacgc tacccaacgc accaccttgc
    14761 tgtttttggc tcattgcatt gagtttttgt tgccagctat ttggtagctg gatttcgtat
    14821 aagccaaaca ttgagcaggc aagcagaata aagacgatag ataaaccaat caacaccggt
    14881 gggctttgta aggcgatttg gaaaggcaag ccgatagccg ctacaattaa gccaagtaag
    14941 gtgtaagtaa ctgccattcc ttgcacatag gcaatactta acagcaatgc tttagcggta
    15001 gaagggcgtt gcttatttcc aattacaatc gcagagagaa gtggtagcat cggcagtacg
    15061 caaggagtga atgctaatcc aacgcctaat acaaaaaacc aaaaaatgga aaaacgattg
    15121 ttagctaaat tttcagccag ttgatcttgc tctgcttttg ccacattttc agggggcgcc
    15181 atattgtcgg tttttgcaaa attttcgctc ttttcgaccg cttgtgcttc cagttgcgcc
    15241 agttcttcag gtgtagcaga gagaatactg tctaatttaa gctcaacccg ttccggtgga
    15301 tagcaaaagc ctttggtaca cccttgatat tccactacga ttgaggcgtt gtctttaatt
    15361 tctttaaacg gtacctttag ccctaagtgg ttgcgataga tttccacctg cccgaaaaat
    15421 tcatcgttat attgctcggc agcgggaaaa gtgatgtcgc ctatctcaat attctgtggc
    15481 gtgacacgga tttctttttt ataaagataa taattgtctg caatatccca atttaactgt
    15541 aatgtttcat tttcattttg gattgaaaaa acaaaggctt cttccgattt taggaatttg
    15601 ggttgagaga aaagatttgc gtgagatgag tttatcatca ataaactcaa gacgagtgaa
    15661 aaaaagtatc taatcatatt gaaatcctat ttttaattgt ctcggcgaaa attatagcgt
    15721 aatcagtatt catattcc
//
LOCUS       DANLZC010000049        15623 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.049, whole genome shotgun sequence.
ACCESSION   DANLZC010000049 DANLZC010000000
VERSION     DANLZC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15623
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.049"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            177..1067
                     /locus_tag="PXE44_001171"
     CDS             177..1067
                     /locus_tag="PXE44_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5588688.1"
                     /translation="MDKLNAISLFCKVIETQSFTQAAQQAQISLPMASKLVAQLEEHL
                     NVRLLQRTTRKITPTEAGLLYYQQCLPILNELKEADASVSNINSTLQGKITMSVPMDF
                     GSRFIAPYLGKFMEDYPNLALHIEFSDRRVDVIAEGFDLVLRIGSLADSSIVAKKIAD
                     SHLVLVASPDYLARFGEPKGLEALVERDCLLYEYHPQWQFSQQGQKMQIKPQGKIYSN
                     NGYALVQMAKAGLGIINIPKFLIKHELKNGELIPIMPFLTQEPLQISLLYPNRRYLSP
                     KVRILIDFFTQLMQEQKEYI"
     gene            complement(1117..1377)
                     /locus_tag="PXE44_001172"
     CDS             complement(1117..1377)
                     /locus_tag="PXE44_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL5588689.1"
                     /translation="MGWIAAIIVGAIIGWIASAVMKSEGGLCKNIIIGILGSSLGRWV
                     FGDVLGIGSAYSAGDFNIVGIFFGVLGAALLIFILRKLSIIN"
     gene            complement(1457..2416)
                     /locus_tag="PXE44_001173"
     CDS             complement(1457..2416)
                     /locus_tag="PXE44_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5588690.1"
                     /translation="MKFHKTLLVTLLSSLSVAALAQDKIQNVAITAIVEHPALDSIRK
                     GVIEELAREGFVDGKNIKIDYQSAQGSTATAAQIARKFVGDKADIIIPITTPSAQPVV
                     AATRSIPIVFSGVTDPVAAKLVKSWEPSGTNVTGISDHKAMAPQVKLIQTLVPDLKAV
                     GYVYSAGEVNSAIVLEELKQEAQKQGFTIVPVAVQRSADIGTAARSLNGKVQAIYISE
                     DNAVVSAYEALHKAALEAKIPVIAADRDTVQRGAVAAYAVNQYDIGVATGKVAARVLK
                     GEKAGTIPIQEVSKLELSINTKTAKELGITIPEVLIKEAKQTF"
     gene            complement(2613..3020)
                     /locus_tag="PXE44_001174"
     CDS             complement(2613..3020)
                     /locus_tag="PXE44_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDL5588691.1"
                     /translation="MKPLRPYLYNAYYNWIIDSDNTPYLLVNAEFPDVDVPVEFVKEG
                     KIILNISPRSIGQYIVADEYISFSARFQGMLRDIYIPFGAMVAIYAQETGDGIMFQEE
                     AYYTEEAYQARVRAVEQPKKIKAKKTSTLKLVK"
     gene            complement(3020..3664)
                     /gene="sspA"
                     /locus_tag="PXE44_001175"
     CDS             complement(3020..3664)
                     /gene="sspA"
                     /locus_tag="PXE44_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDL5588692.1"
                     /translation="MTSSINKRSVMTLFSDKNDIYSHQVRIVLAEKGVPYEIENVMLG
                     SISEDLLELNPYGNIPTLVDRELVLFNPRIIMEYLDERFPHPPLMPVYPVSRGQCRLN
                     MYRIEQDWYSQIDIVNKDPESAEGKKALAQLKEDVLALAPIFAAKPYFMSDDFSLVDC
                     YIAPLLWRMQILGVKFSGATAKPINAYMTRVFQRDSFIQSVGGEAPKHLMDDKD"
     gene            3838..4335
                     /gene="purE"
                     /locus_tag="PXE44_001176"
     CDS             3838..4335
                     /gene="purE"
                     /locus_tag="PXE44_001176"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL5588693.1"
                     /translation="MPNKAEIAIVMGSKSDWATMSEATQILDQFGLSYHVEVVSAHRT
                     PDKLFSFAETAETNGYKVIIAGAGGAAHLPGMIAAKTIVPVLGVPVKSSMLSGVDSLY
                     SIVQMPKGIPVGTLAIGPAGAANAGLLAAQILAAFNPDIAEKLRIFRQTQTQMVLDNP
                     DPRVE"
     gene            4350..5315
                     /locus_tag="PXE44_001177"
     CDS             4350..5315
                     /locus_tag="PXE44_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL5588694.1"
                     /translation="MSKKLLILHTGGTISMKEGEDGKVSPSNENPLLAALERLNHPAK
                     LAQESLFNIPSPHINIEHWQQLKMRIEKAVNEENVDGVVITHGTDTLEETSYFLDLAL
                     SVNIPVVITGAMRSSNELGADGLINLQSAILVALNEESRDKGVLVVMNDEIHNAKFVT
                     KTHTTNVATFQTPTFGPCGLVTKESVIFFQKLTTYERYPIQQVTKANVQLLKAYAGMD
                     SFLLEQLAKSKCDGVVIEALGAGNLPPSCLAGIEALVNANIPIVIVSRAFNGITQDVY
                     DYVGGGKQLKQKGIIFTKGLSGQKARIKLMVLLSQTLDKPLSDYF"
     gene            5334..6422
                     /gene="purK"
                     /locus_tag="PXE44_001178"
     CDS             5334..6422
                     /gene="purK"
                     /locus_tag="PXE44_001178"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL5588695.1"
                     /translation="MQKSAIYPPVYVLGNGQLGRMLRYAGAPLDIEVKPLAFDAPVFD
                     IEPNSIITAEIERWADTPLTQLLGNHPNFVNLNVFGKLADRFTQKSLLDELHLATSKW
                     QLLENAEHWQDIFQHIGEKVVVKRRTGGYDGRGQWIVTQDSIHQITPELFGEVIAEKF
                     IPFEGEISIVGARFRDGSTRFYPISHNLQQNGILRYSVSDVNLPNQALYQQQAEQMLA
                     TVMQKLEYVGVMAMECFVVGDKLLINELAPRVHNSGHWTQLGCAISQFELHLRALLDL
                     PTPALDQIAPSVMVNLIGIEHNKQWLSLPFSQLHWYGKEVRAGRKVGHINLTHTDKNG
                     LIELLEQLRPSLTEDFHSGLDWAINKLK"
     gene            complement(6643..7053)
                     /locus_tag="PXE44_001179"
     CDS             complement(6643..7053)
                     /locus_tag="PXE44_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588696.1"
                     /translation="MISRSLFSKLILFAKIVIFSQLVFMVVIWIAHHIMREYSNPFER
                     FGTLFNNVFLTFIIPMMVSSAVIVYKGYTQKNEDKVFWIYMGFFLIWILGFIYFEPAV
                     YDKSLKISGAGFGAMLSIIICSKLLPKETNKEQL"
     gene            complement(7056..9074)
                     /gene="uvrB"
                     /locus_tag="PXE44_001180"
     CDS             complement(7056..9074)
                     /gene="uvrB"
                     /locus_tag="PXE44_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDL5588697.1"
                     /translation="MSKQGKPFILHSPFKPSGDQPSAIAKLTEGLNDGLAHQTLLGVT
                     GSGKTFTIANVIAKLNRPAMVLAPNKTLAAQLYAEMKAFFPENAVEYFVSYYDYYQPE
                     AYVPASDTFIEKDASINEQIEQMRLSATKSFLERRDTIVVASVSAIYGLGDVDAYMQM
                     MLHLQVGAMIGQREILSRLAELQYTRNDQAFQRSTFRVRGDVIDIYPAESDEVALRVE
                     LFDDEIENLSLFDPLTGHSLGKVPRYTIYPKTHYVTPRERILEAIEQIKVELADRRTY
                     FIKENKLLEEQRIAQRTQFDIEMMNELGYCSGIENYSRYLSGRKEGEAPPTLFDYMPA
                     DGILVIDESHVTVPQIGGMYRGDRARKETLVQYGFRLPSALDNRPLRFEEFERLSPQT
                     IYVSATPGAYELEKNPDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIHKRVAVDER
                     VLVTTLTKKMAEDLTDYLDEHGVRVRYLHSDIDTVERVEIIHDLRMGMFDVLVGINLL
                     REGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYGDRITNSMQKA
                     ITETERRREKQMKYNEEHGITPQALNKKVGELLDIGQSDKPKRGKQAVKKAENSATDY
                     KPKSRKELEKELKTLEQQMRDFAKDLEFEKAAAVRDKLQHLKAYLLEV"
     gene            complement(9313..9549)
                     /locus_tag="PXE44_001181"
     CDS             complement(9313..9549)
                     /locus_tag="PXE44_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL5588698.1"
                     /translation="MSRTTSVTLAEPLSQFVNQMVESGRYGSTNEVISAALKILEAQE
                     QQLIQLRALIDEGLESGVSEETVQSIISKAKRNV"
     gene            9712..10557
                     /locus_tag="PXE44_001182"
     CDS             9712..10557
                     /locus_tag="PXE44_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HDL5588699.1"
                     /translation="MFLLKGNFYAFNQTCFCTFALSGFFVATQAAALDLTKETEAYKQ
                     FVVEQIDQLVTDTEKFVGYLNKGDVQKAKKIYPLARMYFERSEPIAESFGDLDPRIDA
                     RLADLAEEGKTEKDWSGFHKIEKVLWQKNTTKGTKATAEQLLKDVKELRAKIPTAEVT
                     PEPMITGAVDLLNEVSTTKVTGEEEIFSKTDLYDFKANIEGAEKIYEIFKTQLEQIDA
                     KLSTEIGSRFAAVNALLAKHNKSKTGYDYVPYNKLSKADIKALAEAVNQLGEPLAQLG
                     VLLNK"
     gene            10568..11764
                     /gene="efeB"
                     /locus_tag="PXE44_001183"
     CDS             10568..11764
                     /gene="efeB"
                     /locus_tag="PXE44_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDL5588700.1"
                     /translation="MMAETTSRRDFLKHTALVGAGLLSAPAFALESRQISEKSQNQYD
                     FYGKHQAGIVSPAQKHIYFLVLDLDTTDIAKVKTLFQNWTEYSRNLTLGKNVKPYSAN
                     AYVPPADTGEADSLSAQNLTLTFGVSANFFTKLGIEKHKPKELNDLPHFPRDQLQAEY
                     TGGDICIQACADDPQVAFHAVRNLVRVARGEIKMKWSQMGFNSFANQDTPRNLFGFKD
                     GTANAESLKDQENTVWVQQEGWLKGGSYLAVRRVKMFLETWDRTHLQSQEETFGRHRD
                     SGAGIGHQHEFDKLDLAKKDEKGNAVIPEVSHAHLANKTGLKILRRSFSYSSGIDDKG
                     QFDSGLLFVSFQQSPEQFIKIQNALGNVDKMNEYITHIGSGLFACFAGVKDENDYLGK
                     ALFDLI"
     gene            11775..12394
                     /locus_tag="PXE44_001184"
                     /pseudo
     CDS             11775..12394
                     /locus_tag="PXE44_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596976.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+/Pb2+ permease"
     gene            complement(12430..13248)
                     /locus_tag="PXE44_001185"
     CDS             complement(12430..13248)
                     /locus_tag="PXE44_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL5588701.1"
                     /translation="MKYDLHAHSTASDGVLSPTELVQRAVEQGVTMLALTDHDTISGI
                     QEAKNFAQNQPLELISGVEISILWQEKSIHLAALNIDENNEELVKFLDNQAQLREERA
                     VEIGEKLAKVGIANAYEGAKALATGEVTRAHYGRFLYEQGYVRNIEHAFKRYLGMGKP
                     AYVKPRWSSLEDAVNVTHTSGGVISIAHPLRYKMTARWIRRLIADFKQAGGDGIEVSG
                     CGQTPDQRQLLARWAKEFDLYGSVGSDFHYPTGWIELGKSLELPQECKPIWTLF"
     gene            complement(13260..13859)
                     /locus_tag="PXE44_001186"
     CDS             complement(13260..13859)
                     /locus_tag="PXE44_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDL5588702.1"
                     /translation="MTMDNLPLEQHPFDPILPEQATVVMVGTFPPTSEKRCMEFHYPN
                     FQNDMWRIMGNIFYADPNYFRVGEEKRFDPIRIEAFLREKGVALCSSAKTAIRLKGNA
                     SDKDLKIVDPIDMDELLLKLPKLKWLFTTGGLATDTLLGLLPEKHKAPKTNEWVVYPY
                     SATRELYLYRLPSTSRAYPLSLANKTEAYRQFFRTAGLI"
     gene            complement(13856..15403)
                     /gene="sbcB"
                     /locus_tag="PXE44_001187"
     CDS             complement(13856..15403)
                     /gene="sbcB"
                     /locus_tag="PXE44_001187"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDL5588703.1"
                     /translation="MSDKFSFFFYDYESFGVSPALDRPAQFAGIRTDADFNIIGEPVM
                     FFCKQTPDYLPSPEAVMVTGITPQQCNAEGVSEPEFAARIHAEFSRANTCTIGYNNIR
                     YDDEMTRYTFFRNFYDPYEYSWKNGNSRWDLLDLVRACYALRPDGIEWPTDETGMPSF
                     KLENLTKANGIAHENAHDAMSDVYATIAMAKLIKQKQPKLFDFFFNLRSKKEVEKLID
                     TGEMTPLVHVSGMLGNYRGNTAWVVPLAWHSTNQNAVIVCDLSGDMNGLLTENAEVLR
                     QRLYTKRDELAENELPVPLKLVHINKCPILAPAKTLLPENAERLGIDRALCLENLKTL
                     KAQKSLIREKVIEIFNEERTFEPSTNVETTLYDGFFSPADKNNMAILRTLPPEDLANH
                     GLKFEDPRVEALLFHYRARHYPQTLSRAEQIKWQKHCNQQIEAKAAQFAQSIDDLFQQ
                     HHDNPEKVKLLENLTAYAEQISQQQAVIYRQNVAKDEKLLSELNRVAEQPLDKTEKLK
                     MLKELIK"
BASE COUNT         4666 a         3369 c         3082 g         4506 t
ORIGIN      
        1 ttttttgcct tagccggaga ctccacaata actagtgatt ttcccataat ttattaccta
       61 atttttaata attattttaa attgtttgaa attatttctt tttaagttga tttttttaga
      121 cactattttt acgatagcaa gccttaacac aaattacaat ataaagaaga gagaaaatgg
      181 ataaactaaa cgcaatcagt ttattttgta aagtaattga aacccaaagt ttcactcaag
      241 cagcacaaca agctcaaatt tcgttaccca tggcaagtaa attggtggct cagttagaag
      301 aacatctcaa tgtgcgttta ctacaacgaa ccactcgaaa aattacgcca actgaggcag
      361 ggttgctcta ctatcagcaa tgcttgccta ttttaaatga actaaaagag gccgatgcca
      421 gcgtaagtaa tattaactca acgctacaag ggaaaataac aatgtctgta ccaatggatt
      481 ttggctctcg cttcattgcc ccttaccttg ggaaatttat ggaagattac cctaatttag
      541 cccttcatat tgaatttagt gaccgtcggg tagatgtgat tgcagaaggc tttgatttgg
      601 tgctgcgtat tggtagcctt gcagatagtt ccattgtagc gaaaaaaatt gcagattccc
      661 atttggtttt agttgcttct ccagattatt tagctcgatt tggcgaacct aaaggcttag
      721 aagcattagt ggaacgagac tgcctactct atgaatacca tcctcaatgg caattttctc
      781 aacaaggaca aaaaatgcaa attaaaccac aaggtaaaat ttacagtaac aatggttatg
      841 cgttagtaca aatggcaaaa gctggtttgg gcatcattaa cattccaaag tttttaataa
      901 aacacgagct aaaaaacgga gagttgattc ctattatgcc ttttttgaca caggaacctt
      961 tacaaatcag cttactctat ccaaaccgtc gttatctctc tcctaaagtg cgaatcctaa
     1021 ttgatttttt cacacaactg atgcaagagc aaaaagagta tatttaacaa aaaagaagca
     1081 gttataaaac aactgcttcc tttctgaaat acgaaattag ttaattatgc ttaattttct
     1141 caaaatgaaa atcaaaagag ctgcccctaa tacaccaaaa aagataccga ctatattaaa
     1201 gtctccggca ctataagcgg aaccaatacc taatacatca ccaaataccc agcgtccaag
     1261 tgacgaaccc aaaataccaa taataatatt tttgcaaagt ccgccttcac ttttcattac
     1321 agccgaagct atccaaccaa ttatagcgcc aacaataatt gcagcaatcc atcccataat
     1381 attctcctta gctgagttga aatgaatttt tactctacaa gattatgaca aataaaacaa
     1441 atttttattt tttacactaa aaagtttgtt tagcttcttt gattaatacc tctggtatag
     1501 taatgcccaa ttcttttgcc gttttagtat taattgaaag ctctaatttg ctgacttctt
     1561 ggattggaat tgtacctgct ttttcccctt ttaatacacg agcagcaact ttaccggttg
     1621 ccacaccaat atcatattga ttaaccgcat aagctgccac tgcaccacgt tgtactgtat
     1681 cacggtcagc agcaatcacc ggaatttttg cttctaaagc cgctttatgt aaggcttcat
     1741 aagccgaaac aactgcatta tcttcagaaa tataaatagc ctgcactttt ccgtttaggc
     1801 tgcgagcagc agtgccaata tcggcactgc gttgaacagc aaccggcact atagtaaagc
     1861 cctgtttttg agcctcttgt tttaactctt ctaacacaat cgcagaatta acttcacctg
     1921 cactatacac ataaccaaca gctttcaaat ccggtactaa tgtttgaatt aatttcactt
     1981 gtggagccat cgctttgtgg tctgaaatcc ccgtcacgtt agtgccactt ggctcccacg
     2041 attttactaa ttttgcagca accgggtctg tcacgcctga aaacacaatt gggatagaac
     2101 gtgttgcagc aacaacaggt tgtgccgaag gtgtggtgat cgggataata atatctgctt
     2161 tatccccaac aaatttacgc gcaatttgtg ctgctgttgc agtactaccc tgtgcagact
     2221 gatagtcgat tttaatgttt ttaccatcaa caaacccttc tcgggctaat tcttcaatca
     2281 cacctttacg aattgaatct aatgcagggt gttccacaat cgctgtaatt gctacatttt
     2341 gaattttatc ttgagctaat gccgctacag aaagtgaact taacaaagtc actaataagg
     2401 ttttatggaa tttcattttc tctccttaag atcattactg tactatcaca acagtacaaa
     2461 cacaaaacaa agataagcaa tttttagtcg gagtgcaagt ggaatttgat tatttccgtc
     2521 acaaaacaaa caagcggtta aattttgact gatatttgca aataatcaga aaaatttaac
     2581 cgcttgtaat gtgttttgat gaaatagatc tcttatttca ccaactttaa ggtagatgtt
     2641 ttcttcgcct tgattttttt aggctgctct actgctctaa ctcgagcttg ataagcctct
     2701 tcagtgtagt aggcttcttc ttggaacata ataccatcac cggtttcttg cgcataaatc
     2761 gccaccattg ccccaaaagg aatataaata tctcttaaca ttccctgaaa acgagcgcta
     2821 aagctaatgt attcatcagc cacaatatac tgcccgatgg aacgaggcga aatatttaaa
     2881 ataatcttcc cttctttgac aaactctacc ggcacatcta cgtctggaaa ttctgcattc
     2941 actaataagt aaggcgtgtt atcgctatca ataatccagt tatagtaggc attataaaga
     3001 taaggtctta atggtttcat tagtctttat cgtccattaa atgttttggc gcttcgccgc
     3061 caacagactg gataaaacta tcacgttgga atacacgggt catatacgca ttaattggtt
     3121 ttgccgttgc accgctgaat ttaacgccta agatttgcat acgccataat aacggtgcaa
     3181 tatagcaatc aaccaagctg aaatcatcgc tcataaaata aggtttagcg gcaaaaatag
     3241 gcgctaatgc caatacatct tctttaagct gtgctaacgc tttcttgcct tccgctgatt
     3301 caggatcttt attcacaata tcaatttgcg aataccaatc ttgttcaata cgatacatat
     3361 ttaaacggca ctgaccacga gaaacagggt aaaccggcat taatggtggg tgagggaaac
     3421 gctcatctaa atattccata ataatacgag ggttaaacag cactaattca cgatcaacca
     3481 atgtaggaat attaccgtaa ggattcaatt ctaataaatc ttcagaaata ctgcctagca
     3541 taacgttctc tatttcataa ggcacccctt tttctgctaa cacaatacgc acttggtggc
     3601 tgtaaatatc atttttatcc gaaaaaagag tcattaccga acgtttgttt atactactcg
     3661 tcatttatat ctccgatgat taaatttata aaaaataaaa tggttgtcga ttatatcata
     3721 ttttaaccag cagataacag ccctaaaaat tgcttttcac ttcttaaata gctacgcaaa
     3781 cgtttgcttt gtatgctatg attacactgt tttacattag tatcataagg atttaaaatg
     3841 ccaaataaag ctgaaattgc gatcgtaatg ggatctaaga gtgattgggc tacaatgtct
     3901 gaagcaaccc aaatattaga ccagttcggt ttgagctatc acgttgaagt cgtctcagca
     3961 caccgcacac cggacaaact cttttctttt gctgaaactg cagagactaa cggctataaa
     4021 gtgattattg ccggagccgg cggtgcagct cacttgcccg gaatgatcgc tgctaaaacc
     4081 attgtgccgg tgttaggtgt gccggtaaaa agctcaatgt taagtggtgt agatagcctc
     4141 tattcgattg tgcaaatgcc gaaaggtatt ccggtcggta cgctcgcaat cgggccggca
     4201 ggtgcagcaa atgcaggctt attagccgct caaattttag cagcctttaa ccctgatatt
     4261 gcagagaaac tgcgtatttt ccgtcaaacc caaactcaga tggtattaga taatcctgat
     4321 cctcgtgttg aataactatt gtataaaaga tgtctaaaaa attattaatc ctccacactg
     4381 gtggtactat ttcaatgaaa gaaggggaag acggtaaggt ctccccttcc aatgaaaatc
     4441 ctctcttggc tgccttagaa cgtttaaacc acccagcaaa attggcgcaa gaatcgttat
     4501 ttaatattcc ctctccgcat atcaatatcg agcattggca acagttaaaa atgcggattg
     4561 aaaaggcagt gaatgaggag aatgtcgatg gcgtagtaat cacccacggc acagatacct
     4621 tggaagaaac ctcctatttt ctggatttag ccctcagcgt aaatataccg gtagtaatta
     4681 ccggagcaat gcgttccagt aacgaactgg gtgctgatgg cttaatcaat ctacaaagtg
     4741 cgattttagt ggcgttaaat gaagaaagcc gagataaagg cgtgttagtg gtgatgaatg
     4801 atgaaatcca caatgctaaa tttgtgacca aaacccacac cactaatgtg gcgactttcc
     4861 aaaccccaac ctttgggcct tgtggcttag taacgaaaga atctgtcatt tttttccaaa
     4921 aattgaccac ttacgaacgt tatccaattc aacaagtaac taaagctaac gtgcaactac
     4981 tgaaagcata cgccggaatg gatagttttc tgctggaaca attagcgaaa agcaaatgcg
     5041 atggtgttgt gattgaggca ttgggtgcag gtaatttacc ccctagttgt ctggctggta
     5101 ttgaggcttt agtgaatgcc aatattccaa ttgtaattgt ttctcgggca ttcaatggta
     5161 ttacccaaga tgtctatgat tatgtaggcg gtggaaaaca gttaaagcaa aaaggcatta
     5221 tttttaccaa aggcttaagt gggcaaaaag ctcgcattaa acttatggtc ttactcagcc
     5281 aaaccttaga caaaccacta tcagactatt tctaacccat tgagaatacc acgatgcaaa
     5341 aaagtgcaat ctacccacct gtgtatgtat taggcaacgg ccagctcggg agaatgctac
     5401 gctacgccgg agcaccactt gatattgaag tcaaaccgct ggctttcgat gcccctgtgt
     5461 ttgatattga gccaaacagc attattaccg cagaaatcga acgttgggca gatacgcctt
     5521 taacgcaatt actcggtaac cacccgaatt ttgtgaacct gaatgttttt gggaagttag
     5581 ccgatcgttt tacgcaaaaa tcactacttg atgaactcca tcttgcgact tcaaaatggc
     5641 aactgctaga aaatgccgaa cattggcaag atatttttca gcatattggc gaaaaagtag
     5701 tggttaaacg ccgaaccgga ggatatgacg gacgtgggca atggattgta acgcaagact
     5761 cgattcatca aattacacct gaattatttg gcgaagtgat tgccgaaaaa tttatcccgt
     5821 ttgaggggga aatttccatt gttggggcga gattccgtga cggctcaacc cgtttctacc
     5881 ctatttctca caatttacaa caaaacggca ttctacgtta ctccgtctcc gatgtgaatt
     5941 taccgaacca agccctctac cagcagcaag ccgaacagat gctcgctacg gtaatgcaaa
     6001 aattagagta tgtcggcgta atggcaatgg aatgctttgt tgtgggcgac aagctactca
     6061 tcaacgagct tgcccctcga gtgcataaca gcggacactg gacacaactc ggctgtgcca
     6121 ttagccaatt tgagttacat ttacgggcat tattagatct acctacgcca gcactcgacc
     6181 aaattgcccc aagcgtgatg gttaatttaa taggcattga acataacaag cagtggctaa
     6241 gcctcccatt tagccaacta cattggtatg gcaaagaagt ccgagctggt agaaaagttg
     6301 ggcatatcaa cctcactcac accgataaaa acggcttaat tgaactactg gaacaactca
     6361 gaccgagcct aacggaagat ttccattcgg gattagattg ggcaatcaat aagctcaagt
     6421 aagcggtcgg attttgtaac ttttttgcaa atctgtttac ccgatggtgc gagcttcctc
     6481 gctcgtacca aacaagcggt cgacttagtg ctaaaatttg caaaataatt caacataatg
     6541 gctacttccg gtcatcatct tgatctgaaa acccctcagg gtggatatgg ctatcaaaaa
     6601 gcattttctt cctccgagaa aacagatttc ttcacaagaa tcttagagct gctctttatt
     6661 tgtttcctta ggcaataact tcgagcaaat gataatagag agcatagccc caaatcctgc
     6721 acctgaaatc ttcagtgatt tatcataaac tgccggctca aaataaataa atcccaaaat
     6781 ccaaattaaa aagaacccca tataaatcca aaacacttta tcttcatttt tctgggtata
     6841 acctttataa acaataaccg cactacttac catcatcgga ataataaaag tcaaaaacac
     6901 attattgaac agggtaccaa atcgttcaaa cggattactg tactcccgca taatatggtg
     6961 ggcaatccag ataaccacca taaaaacaag ttgagagaaa ataacaattt tggcaaataa
     7021 gattaatttt gaaaataatg aacgagaaat catatttaaa cctccaacaa atacgccttt
     7081 aaatgctgca acttatccct taccgcagcc gctttttcaa attccagatc tttcgcaaaa
     7141 tcacgcattt gttgttccag cgttttaagc tctttttcca gttctttacg ggatttcggc
     7201 ttgtagtcgg ttgcagaatt ttcggctttt ttgaccgctt gtttgccccg ttttggcttg
     7261 tcggattgtc cgatgtcgag caattcaccc acttttttgt tcaacgcttg tggcgtgatg
     7321 ccgtgttctt cgttgtattt catctgcttt tcacggcggc gttcggtctc agtaattgct
     7381 ttttgcattg agttggtaat gcggtcgccg tataaaatcg cttttccgtt gaggtttcgg
     7441 gcggcacgtc cgatggtctg aattaacgag cgttcggaac gtaaaaatcc ctctttatcc
     7501 gcatcaagaa tggcaaccag cgacacttcg ggcatatcta agccctcacg caataagttg
     7561 atccccacca gcacatcaaa cattcccatt cgtaaatcgt ggataatttc gacccgttcc
     7621 accgtgtcaa tgtcgctatg tagataacga actcgtacgc catgttcgtc caaataatcg
     7681 gtgagatctt ccgccatttt tttggtgagt gttgtcacca gcacccgttc gtccaccgcc
     7741 actcgtttgt gaatttcaga cagtaaatcg tccacctgcg tagcaacggg gcgaacttcg
     7801 ataatcggat cgagcaagcc ggttggacga actacttgat ccaccacatc gggatttttc
     7861 tccaattcat acgcccccgg tgtggcagaa acgtaaatcg tctgtggtga aagacgttca
     7921 aactcttcaa atctcaacgg gcggttatcc agtgcagacg gcagacggaa accgtattgc
     7981 accaatgttt cttttctcgc tcggtcgcct cgatacattc cgccaatttg tggcacagta
     8041 acgtggcttt catcaatcac caaaataccg tccgccggca tataatcaaa cagggtcgga
     8101 ggggcttcgc cctcttttct gcccgagaga tagcgtgagt agttttcaat gcccgaacag
     8161 tagcccagct cgttcatcat ctcaatatca aactgcgtac gttgggcgat ccgctgctct
     8221 tccagcaatt tattctcttt gatgaaatag gtgcgacggt cggcaagctc gaccttaatt
     8281 tgctcgattg cctccaaaat ccgttcccga ggggtcacat agtgagtttt cgggtaaatg
     8341 gtatagcgag gcaccttgcc caagctatgc cctgtaagcg gatcaaacag cgacagattt
     8401 tcgatttcat cgtcaaacag ctctaccctt aacgctactt catcgctttc ggcagggtaa
     8461 atatcaatca catcgccccg cactcggaaa gtcgaacgtt ggaacgcctg atcgttgcga
     8521 gtatattgca attcggcaag gcgagagaga atttcacgct gcccaatcat cgcccccact
     8581 tgcaaatgca acatcatctg catataagcg tccacatcac ccaagccgta aatggcagaa
     8641 acggacgcca ctacaatcgt atctcggcgt tccaaaaagg atttggttgc ggaaagtcgc
     8701 atttgctcga tctgctcatt gatagaggca tctttctcga taaaggtatc gcttgccggc
     8761 acataggctt cgggctggta gtaatcatag taagagacga aatactccac cgcattttcc
     8821 ggaaagaacg ctttcatctc ggcataaagc tgggcggcaa gggttttatt cggggcaagc
     8881 accatcgccg ggcgattgag cttggcaatc acattagcaa tggtaaaggt tttccccgag
     8941 ccggttacgc caagtaaggt ttgatgtgcc aagccgtcat ttaagccttc ggtcaatttg
     9001 gcaatcgcac ttggttggtc gccggagggc ttgaaaggag aatggaggat aaagggtttg
     9061 ccttgcttgc tcatagaaaa tcctgtctta aaaaatcggt ttattttagc acaagcaagc
     9121 ggtcgttttt atcgggattt ttgcaaattt attgtaactg cttatattga tccattcttg
     9181 gatctaacac cctaacaata aagatacctt ttgggattgg tttataataa atagaatgtg
     9241 agcggtggta atgacagcgt aacaaagggc gaataaagta acgctaaatc tcgctctgcc
     9301 ctaacagaaa gtttatacat tacgttttgc cttgctgata atagattgaa cggtttcctc
     9361 agaaacaccg ctttctaagc cttcatcaat taaggctctt agctgaatta gttgctgttc
     9421 ttgtgcttcc aatattttta atgcggcact gatgacctcg ttggtcgaac cataacgccc
     9481 actttcaacc atttggttca caaattggct taatggctca gctaaagtta cgctggttgt
     9541 tctactcatt actattccta tcttattgat tattgagcag attgtaatgg ctctaattta
     9601 gataagcaag cggtctattt ttagcaataa tttgcaaaac aaaatgtgat ttacatcaca
     9661 aaatcagcat tattttttgc gaataattcg caataaggat attatgtcgc aatattttta
     9721 ttaaaaggaa atttctatgc gtttaaccaa acttgctttt gcacttttgc actttctggc
     9781 ttttttgtgg ctactcaagc ggcagcttta gacttaacta aggaaaccga ggcttataaa
     9841 caatttgtgg tagaacagat tgaccaactg gttaccgata cggaaaaatt tgtcggctac
     9901 ttaaacaaag gcgatgtgca aaaagcgaag aaaatttacc cgcttgcaag aatgtatttt
     9961 gaacgttctg aaccgattgc agaaagtttt ggcgatttag accctcgtat tgatgcccgt
    10021 ctggcagatt tagccgaaga aggtaaaacc gagaaagatt ggtcaggttt ccataaaatc
    10081 gagaaagtat tgtggcagaa aaacaccact aaaggcacaa aagcaacggc agaacaatta
    10141 ttaaaagatg taaaagagct gcgtgcgaaa attcccactg cagaagttac ccctgagccg
    10201 atgattaccg gtgcggtaga tttattaaac gaagtttcta ctactaaagt aaccggtgaa
    10261 gaagaaattt tctctaaaac cgacctttac gattttaaag ccaatattga aggtgcagaa
    10321 aaaatctatg agatttttaa aacgcaatta gagcaaattg atgccaaact ctcaacagaa
    10381 atcggctctc gttttgcggc tgtcaatgcg ctgttagcaa aacacaataa atctaaaacc
    10441 ggttatgatt atgtgcctta taacaaactg tcaaaagcgg atattaaagc gttagcagag
    10501 gctgtaaacc aattaggcga gccgttagct caacttggcg tacttttaaa taaatagtgg
    10561 agcaaaaatg atggcagaaa ccacttctcg ccgtgatttt ttaaaacata ccgcattagt
    10621 aggagcaggc ttattgtctg ctcctgcttt cgctttggaa tcacgacaaa tatctgaaaa
    10681 atctcaaaat caatacgact tttacggcaa acatcaagct ggtattgtta gccctgcaca
    10741 aaaacatatc tactttttag tgctggattt agacaccact gacattgcca aagtaaaaac
    10801 attgtttcaa aactggacgg aatatagccg taacctcacg cttggcaaaa atgtgaaacc
    10861 ttatagtgct aatgcttatg ttccaccggc ggataccggt gaggcagata gcctttcggc
    10921 acagaatctc actcttactt ttggcgtcag tgccaatttc tttacaaagc tcggcattga
    10981 aaaacataag ccgaaagaac tgaatgattt accgcatttc cctcgtgatc aactacaagc
    11041 agaatacacc ggtggcgata tttgcattca agcctgtgca gacgatccgc aagtggcttt
    11101 ccacgctgtg cgtaatttgg tgcgtgttgc ccgtggtgaa atcaaaatga aatggtcgca
    11161 aatggggttt aactcctttg ccaatcaaga cacacctcgt aacttattcg gttttaaaga
    11221 cggcactgcc aacgcagaaa gtttaaaaga tcaggaaaat acggtatggg tgcaacaaga
    11281 gggctggctc aaaggcggtt cttatttagc cgttcgccgt gttaaaatgt tcttggaaac
    11341 ttgggatcgc acccatctcc aatcccaaga agaaaccttc ggtcgtcacc gtgattccgg
    11401 agccggaatc gggcatcagc acgaatttga taaattagat ttagccaaaa aagacgaaaa
    11461 aggcaatgca gtaattccgg aagtttcaca cgctcatctt gccaataaaa cggggctgaa
    11521 aatcctacgc cgttcattct cctattcaag cggtattgat gataaagggc aatttgattc
    11581 agggttgttg tttgtctctt tccaacaaag tccggagcaa tttatcaaaa tccagaacgc
    11641 tctcggcaat gtggataaaa tgaatgaata catcactcat atcggcagtg gtttatttgc
    11701 ctgttttgcc ggtgtgaaag atgaaaatga ctaccttggc aaagcgttgt ttgatttaat
    11761 ttagggaaaa ggaaatgaaa ttaaaagcat tagtgctaat tccgttactc ttttcgagta
    11821 caggattgct tgccaaagtg aatgtcagcc ccttatttgt tcagctttcc gatgcaatgg
    11881 cagagagtaa aaaaggggaa tttgcaaaat ccagccaaaa tttgaccgct ttacagcacg
    11941 attttgagca gattagccag cacgattccc tcgccggagt tgaagtacag accgctctga
    12001 aaaaagcgat tgaaacaccc aatgctgaaa acttagagag cctttctaaa aactctatgc
    12061 gtttgaaaaa gaacaaaacc ctagcggtta tgctgaaaaa catcaggatt ttgtggcaaa
    12121 aatgacgccg ctttatgcag aacttgctca aaccgtgcca actcaagaaa tcgccaaaat
    12181 caaatgggcg gcttatcagt ttggtaagaa ttgggtaaaa caggaaaaag cagtacggga
    12241 aatcagtctt ccccattatg gcaaatttga gcgtattctt ggtttaatgc gaattaatct
    12301 gaacgctgaa aagccggata tggcaaaaat cagtgaactg gtttccgaac taggtgtggt
    12361 aatggcagac tttaagcaag ttaaagtgaa atagtaaaaa atcggggcaa attatttgcc
    12421 ccgataacac taaaataacg tccaaatcgg tttacattct tggggtaatt ccaaactttt
    12481 accgagctca atccagccag ttgggtagtg gaaatcagaa ccgaccgagc catataaatc
    12541 aaactctttt gcccaacggg caagtaattg ccgttgatca ggtgtttgtc cgcagcctga
    12601 tacttcaatg ccatcgcctc cggcttgttt gaaatctgcg attaaacggc gaatccaacg
    12661 agcagtcatt ttatagcgta gcgggtgagc aatcgaaatc acaccgcctg aggtgtgggt
    12721 aacattcacc gcgtcttcta aactactcca tctcggtttc acataggcag gtttccccat
    12781 tcccagataa cgcttaaagg cgtgttcaat attgcggaca tagccttgtt catacaaaaa
    12841 acgcccataa tgtgcccgag ttacttcccc tgttgccaac gcttttgccc cctcataggc
    12901 atttgcaatc ccgacttttg ctaatttttc accaatttcg accgctctct cttcccttaa
    12961 ttgggcttgg ttatccaaaa acttcaccag ctcctcgttg ttttcatcaa tattcaacgc
    13021 cgcaagatga atactttttt cttgccataa aatcgaaatt tccaccccag agataagctc
    13081 aagcggttga ttttgggcaa aatttttcgc ttcttgaatg cccgaaatcg tatcgtgatc
    13141 agttaaagct agcattgtta ccccttgctc taccgcacgt tgtaccaatt cagtgggaga
    13201 aagtacgcca tcagaggcgg tactgtgggc gtgtaaatca tatttcattt tatttatcct
    13261 taaattaagc cggcagtcct gaaaaattgg cgataggctt cggttttgtt ggctaagctg
    13321 agtggataag ccctcgacgt cgaaggcagg cgatataaat aaagttctct tgtggcggaa
    13381 taaggataga ccacccattc gttggttttc ggagctttgt gtttttccgg caataaaccg
    13441 agcaacgtat cggttgccaa accacctgtg gtaaatagcc atttcagttt gggtaatttg
    13501 agcaacagtt cgtccatatc gataggatca acaattttta aatctttatc tgaagcgttt
    13561 ccttttaaac gaatcgctgt ttttgccgaa gaacagagcg caacgccctt ttctcgcaaa
    13621 aacgcctcaa tccgaatcgg atcaaagcgt ttttcttcac caactcgaaa atagttaggg
    13681 tcagcataaa aaatattgcc cataattcgc cacatatcat tttgaaaatt cgggtaatga
    13741 aattccatac aacgcttttc cgatgtaggt ggaaaagtgc cgaccattac caccgttgct
    13801 tgttcaggta aaatagggtc aaacgggtgc tgttctagcg gtaaattatc cattgtcatt
    13861 taatcaactc ttttaacatc ttgagctttt cggttttatc taggggctgt tccgctactc
    13921 tgttaagctc gctcagcaat ttttcatctt ttgcaacatt ttgacgataa atgaccgctt
    13981 gttgttgcga aatctgctcg gcgtaagcgg tcaaattttc taataatttt accttttccg
    14041 ggttgtcgtg atgttgttgg aacaagtcgt caatcgattg ggcgaactgc gctgcttttg
    14101 cctcaatttg ctggttgcaa tgtttctgcc atttgatctg ctcggctcga ctgagtgttt
    14161 gtggatagtg ccttgctcgg taatggaata gtaacgcctc tactcgaggg tcttcaaatt
    14221 tcaagccgtg gttggctaaa tcttcaggcg gtagcgtgcg taaaatcgcc atattatttt
    14281 tatccgcagg cgagaaaaag ccgtcataca aagtggtttc tacattggtc gaaggctcaa
    14341 acgtgcgttc ttcattaaaa atttcaatca ctttttcccg aatcagcgat ttctgtgctt
    14401 ttaaagtttt caaattttcc aaacataaag ctctgtctat gcctaaacgt tctgcatttt
    14461 caggcagtaa ggtttttgcc ggtgctaaaa tcgggcattt attaatatgc actaatttta
    14521 acggcacagg taactcattt tcggctaatt catctcgttt ggtgtataaa cgctgacgta
    14581 aaacctctgc attttcagtc aataagccat tcatatcgcc cgataaatca cacacaatta
    14641 cggcattttg attagttgag tgccaagcca gtggcacaac ccaagcggta tttcctcgat
    14701 agttgcccag cataccggaa acgtgcacaa gcggtgtcat ttcaccagtg tcaattaact
    14761 tctctacttc ttttttactg cgtaaattaa agaaaaaatc gaacaacttt ggctgttttt
    14821 gcttaattaa ttttgccatt gcgatggtgg catacacatc agacatcgca tcgtgtgcat
    14881 tttcatgggc aatgccattg gctttggtga ggttttccaa tttaaaactt ggcataccgg
    14941 tttcatctgt tggccattca ataccatcag gtcttaacgc ataacaggca cggactaaat
    15001 ctaacaagtc ccaacgagaa tttccgtttt tccagctata ttcgtaagga tcgtaaaaat
    15061 tgcggaagaa ggtataacgg gtcatctcat catcataacg aatattgtta taaccgatag
    15121 tacaagtatt cgctcggcta aattcagcgt gaattcgggc tgcaaattcc ggctcagata
    15181 caccttcagc gttacattgt tgcggtgtaa tcccggtaac catcaccgct tcggggctgg
    15241 gtaaataatc cggcgtttgc ttacaaaaaa acattaccgg ttcaccgata atattaaaat
    15301 ccgcatcggt tcgaataccg gcaaattgtg ccggacgatc taacgccgga ctcacgccga
    15361 aactttcata atcgtaaaag aaaaaagaga atttatcgct cattcgttca atttgttaga
    15421 aaaaattggg ctgattgtag cacagtaagg cagaaaggag ggaatacaag cggtcaattt
    15481 ccgttagaaa tttgcaaatg ctaaaataaa aaaagcccct aatcaaaaaa gcgaaaaggg
    15541 gcggaggtct tacctaatga taatattatt tttataaatc aattagttaa gatgtttttg
    15601 tcgtaaaaat atcgagatta tta
//
LOCUS       DANLZC010000050        15612 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.050, whole genome shotgun sequence.
ACCESSION   DANLZC010000050 DANLZC010000000
VERSION     DANLZC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15612
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.050"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            115..852
                     /locus_tag="PXE44_001188"
     CDS             115..852
                     /locus_tag="PXE44_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009020527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5588704.1"
                     /translation="MRGGKQLAKEQYDVVIDPTVTLRNRDLLFLRTISAKHYVGYQKQ
                     KYRLFDLNVEDRKQHFSEIYQDALALIGFENIETQYDVPLNATSNQSIKHYLYENQIR
                     NYIALNLFGAGSARRFSEQKINELLSCLKQHSDKPIVLLTFPEVTAKLCSFTKKYKNI
                     FVYEKTKSVFDSIELIRFADLLISPDTATVHIASGLSKKIIAFYSSDEQNFIHWHPNN
                     QAETHILRFRKSVNDLDFAAIKPEWLK"
     gene            854..1744
                     /gene="ilvY"
                     /locus_tag="PXE44_001189"
     CDS             854..1744
                     /gene="ilvY"
                     /locus_tag="PXE44_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDL5588705.1"
                     /translation="MDFQSLKLFLDIAQSRSFIRSAEKNYMTASTLTRHIQRIEEELG
                     QPLFLRDNRQVRLTEAGERFLAFAQQGWAEWQQLQNQLSPAQGDLEGELKVFCSVTAS
                     YSHLPPILGKFRQKYPKVDIKLSTGDPALALEQVQSLAADISVAGKPEFLPNNIVFHY
                     IDDIQLSIIAPRVASQATQFLQQSPIDWKNIPFILPVNGPVRKRIDRWFKEQKIKDPI
                     IYATVSGHEAIVPMVALGFGVALLPDIVIKHSPMNNQVSYVNLPSPITPFELGICVQQ
                     KRLQEPIINAFWQLLSDNSA"
     gene            complement(1878..4280)
                     /gene="lon"
                     /locus_tag="PXE44_001190"
     CDS             complement(1878..4280)
                     /gene="lon"
                     /locus_tag="PXE44_001190"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDL5588706.1"
                     /translation="MAPRKKKAIELPLLPLRDVVVFPYMVMPLFVGREKSIQALRSAM
                     DSNKQLFLVTQQDPNKEEPNAEDMYGVGVIANIIQMLNLPDGTVKVLVEGQTRAKIEQ
                     IHDDENGFWAAIQPIYSEYDDENEELKAIAKTTLTEFENYVKNNKKIPAEIIAKLQKI
                     TLEDRLADTIASNLIAPVKKKQELLEQPNLIARFEALLIAMATEMDTLETETRIRNRV
                     KQQMEKNQRDYYLNEQIKAIQKELGNGDDVEQSELDKLKEKIDAAKLPTEVKDKLNTE
                     FKKLKAMPQSSSEATVVRGYIDWILQMPWHKKSAVKKDLAQAQEILDKDHYGLERVKE
                     RIVEYLAVQSRLNKLKGPILCLVGPPGVGKTSLGQSIANATGRKYVRMALGGVRDEAE
                     IRGHRRTYIGSMPGSLMMKMAKVGVRNPLFLLDEIDKMAQDMRGDPASALLEVLDPEQ
                     NKAFNDHYLEVDYDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKMHIARGHL
                     LAKQQENNGLKEGELTIEDSAILSIIRYYTREAGVRSLEREIAKICRKAVKALVLDKK
                     LKSITVSEKNIEEYLGVKRFDYGKMDSQNRIGEVTGLAWTEVGGDLLTIETASVAGKG
                     KFSYTGSLGDVMKESIQAAMVVVRSRAEKLGIAEDFHEKRDIHVHVPDGATPKDGPSA
                     GIAMCTALISSLTGNPVRKEVAMTGEISLRGKVLPIGGLKEKLLAAHRGGITTVIIPK
                     ENEKDLEEIPENAKAALDIHAVETIDEVLALALENPPMGIEILSKAEIKVKKSRAKAT
                     VQ"
     regulatory      4484..4602
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4778..5875
                     /gene="ribD"
                     /locus_tag="PXE44_001191"
     CDS             4778..5875
                     /gene="ribD"
                     /locus_tag="PXE44_001191"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDL5588707.1"
                     /translation="MTDVQYMAYAITLAEKARGWTSPNPLVGCVIVKNGNIIAEGYHQ
                     KAGSWHAERNAILNCEEDLTGATAYVTLEPCCHHGRTPPCSELLIERGIKKVFIGSRD
                     PNPLVSGKGAAQLRAAGIEVVEDFMRTECDALNPIFFHYIQTKRPYVLLKYGMTADGK
                     IATYTGNSKWITGEAARANVQKSRHEYSAIMVGVETVLADDPMLNSRMENAKQPVRIV
                     CDSQLRTPLDSQLVQTARQYRTIIATLNNDAKKQAEYTACGVEILVTGERDKRVDLAD
                     LLQKLGELGIDSLLIEGGSQLNFSVLEQGLVNRVHCYIAPKLIGGKAAKTPIGGKGIG
                     ELAQAVKLRLKSTELLGEDILLDYEVDLLRN"
     gene            6092..6739
                     /gene="ribE"
                     /locus_tag="PXE44_001192"
     CDS             6092..6739
                     /gene="ribE"
                     /locus_tag="PXE44_001192"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDL5588708.1"
                     /translation="MFTGIIEEVGQIAQIKKQGEFVVVTVKAKKILTDVHLGDSIAVN
                     GVCLTVTSFTQEQFTADVMSETLKRTSLGELSINSSVNLERAMAANGRFGGHIVSGHI
                     DGTGVVAEITPADNSTWYRIKAEPKLMRYIIEKGSITIDGISLTVVDVDAESFRVSII
                     PHTIKETNLGTKKIGSLVNLENDIVGKYIEQFLLKKEPENPQSKITADFLKNAGF"
     gene            6758..7957
                     /locus_tag="PXE44_001193"
     CDS             6758..7957
                     /locus_tag="PXE44_001193"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDL5588709.1"
                     /translation="MFKFSTVEEAIQAIAQGKIILVSDDADRENEGDFICAAEFATPE
                     NINFMAKYGKGLICTPISTEIAKKLNFHPMVLENEDNHQTAFTVSVDHIQTGTGISAF
                     ERSLTCLKMLDENAKPEDFRRPGHVFPLVAKDGGVLVRNGHTEATVDLARLAGLKPAG
                     LCCEIMAEDGTMMQMPELQAFAQAHNMPFITIQQLQEYRRKHDSLVNVVSVVKMPTKY
                     GEFKAHSFVETISGKEHVALVKGDIGDGENVLCRIHSECLTGDAFGSQRCDCGQQFAA
                     AMNQVEQEGRGVVLYLRQEGRGIGLINKLRAYELQDRGMDTVEANLALGFKDDEREYY
                     IAAQMFEKLGVKSIRLLTNNPAKIEGLIEQGLNVVAREPIMVEPNEHDLEYLKVKQHK
                     MRHMFNF"
     gene            8163..8627
                     /locus_tag="PXE44_001194"
     CDS             8163..8627
                     /locus_tag="PXE44_001194"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDL5588710.1"
                     /translation="MATFQGNYIATGLKFGIVATNWHKIFIDQLLNGATDKLLRHGVE
                     EKNIDTVWVPGALEISIAAKKMAQSGRYDAIICLGAVVRGATSHYDVVVNESAKGISS
                     SALETGVPIINGILTVENLEQAIERSGTKAGNKGEECAMVAIEMANLLKALA"
     gene            complement(8673..10181)
                     /gene="lnt"
                     /locus_tag="PXE44_001195"
     CDS             complement(8673..10181)
                     /gene="lnt"
                     /locus_tag="PXE44_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDL5588711.1"
                     /translation="MNFAKNPPFLTACLLAIILGAIGTFAYSPFDIWILAFASAAGLV
                     WSATLANRKTALWATFAWSIGYFCTGVNWVSVSMTQFGGVPTIVSYIAVFLLACYLAI
                     YNLLFSVVSHRLKLNNPFALAAIFTFTEYLRGVVFTGFPWLQFGYSLIDSPFAGIAPI
                     LGVEGLTFLVIVASSYLVKIIQQENKTVPSLMVLAVIFGLSFATRFLQFVQIDTEKQP
                     LVVSLVQGNIEQKMKWDPQHFNHSVRTYEQLILPLLGNSELIILPESAIPALENQISP
                     LLGSLDHLARQHGSEIIIGTLYQNEQEELFNSSVLLGEKEREYALHSNPRYNKHHLVP
                     FGEYVPFGSVLDWMREVFILPINLSQGSFVQPSFLAKNNRFNMAICYEIIFGDQLQQN
                     QKSQNSDYLLTITNDAWFGSSIGPWQHFQMARMRALELGKPLLRAANTGITAIVDANG
                     KVIEQLPQFTADALTATIVPTKGDTPFKQLGHWLIYSFSLFCVIASLLLRRK"
     gene            complement(10240..11139)
                     /gene="corC"
                     /locus_tag="PXE44_001196"
     CDS             complement(10240..11139)
                     /gene="corC"
                     /locus_tag="PXE44_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249295.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDL5588712.1"
                     /translation="MSDEQQSVNTTEKSEKKSILHSLFSGLFQNEPKNREDLVEVIRD
                     SVENELIDSDTKEMIEGVMEISELRVRDIMIPRAQIVFIDANEPLDVCIDLIIESAHS
                     RFPVIRGEKDTIEGILHSKDLLKYLRSDSEPFNLQQVLRPAVIVPESKRLDRMLKEFR
                     SERFHMAVVVDEFGAVSGLVTIEDILEQIVGDIEDEFDEEEVKPIRQLSRHTYAVLPL
                     TDIKKFNEFFATDFNDEEVDTIGGLVMQAFGYFPKRDEQIELNGIDFKVTSANSRQLI
                     QLRVTLSDEQLEKMEKTSPNEEE"
     gene            11277..11885
                     /locus_tag="PXE44_001197"
     CDS             11277..11885
                     /locus_tag="PXE44_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDL5588713.1"
                     /translation="MKLTKIALSLTALVVSSMSVAGTLSTSSMVDILAFDGQRVKSGT
                     TSVQMSENKTHQVVVEVGGTVEGDFFTSDQIILTFQGSAENAKLETPRLNSKLDLRKF
                     QENQTVAIKTDSGKVISHKQDFLKGEGFLAKTRVEDNLTKYNLAKKTASVEQFATAPL
                     EAKGQIVVDTNKVSQEELQLLFRKADKDTQKRFLEWAKKNIK"
     gene            complement(11966..12349)
                     /locus_tag="PXE44_001198"
     CDS             complement(11966..12349)
                     /locus_tag="PXE44_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588714.1"
                     /translation="MKTLKLGCIFSLALMLSSCTLTPEQQAERKAKQIRAEQDLQVQL
                     AKQCDVETAELMYQQFNPPLSQTEQEEKAFKKRYAEKVNAPMFQACYKLAWQNYKAQV
                     ELEEIRWNYDRDYMYRGWRYCYYCW"
     gene            complement(12379..12948)
                     /locus_tag="PXE44_001199"
     CDS             complement(12379..12948)
                     /locus_tag="PXE44_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDL5588715.1"
                     /translation="MNETTINDFSLLCRLFGNLFYRHPTDPILAGTFNWLAQGGLRQV
                     WALNTDSQSENALTLLQKNANPTQLAASYQALFAENGAISPTISAYGLSVNNFVAFRQ
                     ERAMPELENADHIGLLFLTASWIEDNLDSALAQKILFEQFLLPCAGKFLGKIEAFDNG
                     FYKALAQLSRDALSAMADELEESELEAVE"
     gene            13007..13852
                     /gene="nfo"
                     /locus_tag="PXE44_001200"
     CDS             13007..13852
                     /gene="nfo"
                     /locus_tag="PXE44_001200"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL5588716.1"
                     /translation="MKYIGAHVSASGGVENAVLRSVEIGANAFALFTKNQRQWQAPAL
                     KAETIEKFKRFCKVHHFSAEQILPHDSYLINLGNPEAEALEKSRVAFIDEMSRANQLG
                     LKLLNFHPGAHLKQISEQDCLARIAESINIAVEKVPNVIAVIENTAGQGSNLGWHFDH
                     LATIIEQVEDKSRVGVCLDTCHLFSAGYDISSYEKSEAVFNDFNQTVGFHYLRGMHLN
                     GSKTPLGSRVDRHHTLREGTIGTEVFAFIMQNMHFNHIPMILETISPEIWADEIKFLR
                     TLELK"
     gene            13980..14552
                     /locus_tag="PXE44_001201"
     CDS             13980..14552
                     /locus_tag="PXE44_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5588717.1"
                     /translation="MKKNLFATTFKPVFIASILSVFGGYSSIAVASLSSKPATPNQII
                     ASKAERLAKSSSVFTNRANLPTSSAHTANYSGRINSVYHNWVGTRYRLGGTTKAGIDC
                     SAFVREVMGKAFSKDMPRSTAEQRHVGHSISKSDLRPGDLVFFRKNNHVGVYIGNGKF
                     VHASSSRGVTTSSLSESYWSRNYTQSRRVI"
     gene            complement(14619..15077)
                     /locus_tag="PXE44_001202"
     CDS             complement(14619..15077)
                     /locus_tag="PXE44_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5588718.1"
                     /translation="MASIRQISDQTAAYQPIPLYRDFNSSRSVKLDLNSLCIKRPRDT
                     FFIHVKNPNLIAWGIELDDLLIVEQTNQYLVNDLLVLEKDGEYKFYQFFNEIQNGEGI
                     AEKILFSLDVSESNLRITDWAEVKIAGVITNVVHQMRTRGAFTQAKQRVA"
     gene            complement(15251..>15612)
                     /gene="grcA"
                     /locus_tag="PXE44_001203"
     CDS             complement(15251..>15612)
                     /gene="grcA"
                     /locus_tag="PXE44_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDL5588719.1"
                     /translation="ESSNSNLLNSFWLLDEAKNEARCLAAKAGFEEDQVVAISELGDI
                     AYREVPVNVAPTIKVEGGQHLNVNVLRRETLEDAIQNPEKYPQLTIRVSGYAVRFNSL
                     TPEQQRDVITRTFTESL"
BASE COUNT         4629 a         3061 c         3304 g         4618 t
ORIGIN      
        1 tctccccaaa cgcaaatagg cgtggtttgc tcacagaaaa accgatattt atttgagaat
       61 aatccttata ttgataagct ctatttggtt aagacaaaaa gtattcctga ttatattcgt
      121 ggtggcaaac aacttgcaaa agagcagtat gatgttgtaa tcgatccgac tgttacttta
      181 cggaatcgtg atctcttatt tttacgcact atatcagcca aacattatgt aggttatcag
      241 aaacaaaaat atcgattatt tgatctaaat gtagaggata gaaaacagca tttttcagag
      301 atttatcaag atgcgttagc tttaatcggg tttgaaaata tagaaactca gtatgatgtg
      361 ccactaaatg ccacaagcaa ccaatccata aagcattatt tatatgaaaa ccaaattaga
      421 aattatattg cgttaaatct ttttggtgca ggttcggcaa gacgttttag tgagcagaaa
      481 attaatgaac tgctgagttg cttaaaacag catagtgata aaccgatagt cttacttact
      541 tttcctgaag taacggcaaa attgtgttcc tttactaaga agtataaaaa tatatttgtc
      601 tatgagaaaa ccaaaagcgt ttttgattca atagaactga ttcgttttgc ggacttatta
      661 atttcacctg atactgcaac cgtgcatatt gcctcaggct tgagtaaaaa gataattgct
      721 ttttacagta gcgatgaaca gaattttata cattggcatc cgaataatca ggctgaaacc
      781 catattctcc gttttagaaa aagcgtgaat gatttagatt ttgctgcaat aaaaccggag
      841 tggctaaaat aatatggact tccaaagcct taaacttttc ctagatatag cccaaagccg
      901 tagttttatt cgcagtgcgg agaagaatta tatgacggct tccacactta cccgccatat
      961 tcagcgtata gaggaggagc tcgggcagcc gttgtttctg cgtgataatc gacaagtgcg
     1021 tttaactgaa gcaggggaaa ggtttttggc gtttgctcaa caaggttggg cagagtggca
     1081 gcagttgcaa aaccagttgt cgcctgctca aggtgattta gaaggagagt tgaaagtgtt
     1141 ctgctcggta acggcttctt acagccattt accgccgatt ttgggaaagt ttcgccaaaa
     1201 atatccgaaa gtggatatta aactgagtac cggtgatccg gctttggcat tggaacaggt
     1261 tcagtcattg gctgcagata tttcggttgc gggtaagccg gaatttctgc cgaataatat
     1321 tgtgtttcac tatattgatg atattcaact ttcgattatt gcccctcgtg tggcgagcca
     1381 agccactcaa tttctgcaac agtcgccgat agattggaaa aatatcccct ttattttgcc
     1441 ggtgaacgga cctgtacgaa aacggattga ccgttggttt aaagagcaaa aaatcaaaga
     1501 cccgattatt tatgctaccg tctccggaca cgaagcgatt gtgccgatgg ttgcactagg
     1561 ctttggggtc gcactgctac cggatattgt gattaagcat agcccaatga ataatcaagt
     1621 ttcttatgtg aatttaccct cgccgatcac tccgtttgag ctgggaattt gcgttcagca
     1681 aaaacgcttg caagagccga tcatcaacgc attttggcaa ttattgtctg ataattcagc
     1741 ataacaagcg gttagatttt gctaaatttt tgcaaaaaat agagcaaaac cgaccgcttg
     1801 tagataaaac tttcagataa aaaataaggt gcgtgattgt gatctgcgca ccttatgttg
     1861 ttttagatta ttgcacttta ttgcactgtg gctttagctc gcgatttttt caccttgatc
     1921 tcggcttttg acaagatttc aatccccatt ggcgggtttt ctagtgccag agctagtacc
     1981 tcgtcaatgg tttctactgc gtgaatgtcc aacgctgctt ttgcattttc cggaatttcc
     2041 tctaagtctt tttcattttc tttcggaata atcaccgtcg taataccacc acgatgggct
     2101 gccagtaatt tctcttttaa gccgccgatt ggcaatactt tgccacgcaa gctgatttca
     2161 ccggtcatcg ctacttcttt acgcacaggg ttacctgtta ggctggaaat taatgcagta
     2221 cacattgcaa tccctgcact cggaccatct ttcggtgttg caccgtccgg cacgtgaacg
     2281 tgaatatccc gtttttcgtg gaagtcttca gcaatgccta atttttctgc acgagagcgt
     2341 acaaccacca ttgccgcttg aatagactct ttcattacat cgcctaatga accggtgtaa
     2401 gagaatttgc ctttacctgc aacagaagcg gtttcaatgg ttaataaatc accgccaact
     2461 tctgtccacg ctaaaccggt cacttcgcca atgcggtttt ggctatccat tttgccgtag
     2521 tcaaaacgtt ttacgcctaa gtattcttcg atatttttct cagaaactgt aatggatttt
     2581 aatttcttat ccaatactaa agctttcacg gctttacggc agattttcgc aatttcacgc
     2641 tcaaggctac gcacgcctgc ttcacgggtg tagtagcgga taatacttaa aattgcactg
     2701 tcttcaatgg tcagctcacc ctcttttagt ccgttatttt cttgctgttt agccagtaaa
     2761 tgaccacgag caatgtgcat tttctcatct tcggtatagc ctgaaagacg aatcacttcc
     2821 atacggtcga gcaatgctgg cggaatgttc attgagtttg aggttgccac aaacattaca
     2881 tcggataaat cgtaatccac ctctaaataa tggtcgttaa aggctttatt ttgctcagga
     2941 tctaacactt caagcagagc agaagccggg tcgccacgca tatcttgagc cattttgtcg
     3001 atttcatcta acaagaacag cggattacgc acgccgactt tcgccatttt catcattagg
     3061 ctacccggca ttgagccgat gtaagtacgg cgatgcccac ggatttccgc ttcatcacgc
     3121 acgccaccta acgccatacg cacatattta cgccctgtgg cattggcaat agattgccct
     3181 aatgaggttt taccaacccc cggcggacca actaagcaca aaatcggacc ttttaattta
     3241 tttagacgac tttgcactgc taagtattct acaatccgct ctttcactcg ttctaagccg
     3301 tagtggtctt tatccaaaat ctcttgagct tgggctaaat cttttttgac cgctgatttt
     3361 ttatgccacg gcatttgtaa aatccagtca atataaccac gcacaacggt agcttcggaa
     3421 gagctttgcg gcattgcttt gagctttttg aactccgtat tcagcttgtc ttttacctcg
     3481 gtcggtaatt ttgcagcgtc aattttctct ttaagcttat ctaactcgct ttgttcaacg
     3541 tcatcgccat tacctaactc tttttgaatc gctttaattt gctcgttgag gtagtaatca
     3601 cgttggtttt tttccatttg ctgtttaaca cgattacgaa tccgagtttc ggtttcaagc
     3661 gtatccatct cagttgccat cgcaattaac aaggcttcaa aacgggcaat cagatttggt
     3721 tgctccaata attcctgttt tttcttcacc ggagcaatta agttagaggc aatggtatcc
     3781 gccaagcggt cttccaacgt gattttttgc agtttagcaa taatttccgc cggaattttc
     3841 ttgttatttt taacgtaatt ttcaaattcg gttaaagtgg ttttagcaat cgcttttaac
     3901 tcttcgtttt cgtcatcata ttccgaatat attggctgaa ttgccgccca gaagccgttt
     3961 tcatcatcgt gaatttgctc gattttggca cgggtttgtc cttctaccag cactttaact
     4021 gtgccgtccg gcaggtttaa catttgaata atgttggcaa ttacacctac gccatacata
     4081 tcttctgcat tcggctcttc tttatttgga tcttgctgag tgactaaaaa cagttgttta
     4141 ttggaatcca tcgccgagcg tagggcttga attgattttt cacgtcctac aaataacggc
     4201 ataaccatat aaggaaaaac caccacatca cgcaatggga gtagtggtaa ttcaatcgct
     4261 ttcttttttc ttggtgccat aaatttgctc tctctatcaa aatataatcc cccattatga
     4321 gggcgaattt gggaaaaaca agggaaaatt tgatttatct taaaaaaact cgaatagtca
     4381 aaacagtaga tgttggcgat ttaatcaatt gttaaaatca gggtaattag cttaatttcc
     4441 cgcttgacat caaatcgtaa tgcgagcaaa atcccctgca tattttaatc ttcagggcag
     4501 ggtgaaattc ccgatcggcg gtaaagtccg cgagccgagc aatcggcagg atttggtgaa
     4561 attccaaaac cgacagtata gtctggatga aagaagagtc aaaaaccgca aaaaataccg
     4621 cacaagcggt cattttttgt gtcttttttg caagccttga gtcgtgagat ttcaaggctt
     4681 cattgttttg caggcggcgt ttgcaaattt tttactaaaa acgaccgctt gtattgcaat
     4741 aatcctttca ttacgccctc aatttatcaa aatgtatatg acagatgttc aatatatggc
     4801 ttatgccatc acccttgccg aaaaagcacg gggttggaca agccctaatc cgctagttgg
     4861 ctgtgtgatc gtgaaaaacg gcaacattat cgccgaaggg tatcaccaaa aagccggtag
     4921 ttggcacgct gaacgcaatg cgattttaaa ctgcgaggaa gatctgacgg gggcaaccgc
     4981 ttatgtaacc ttagagcctt gttgccacca cgggcgaacc ccgccttgtt ccgaactgct
     5041 gattgaacgt gggatcaaaa aagtgtttat cggctctcga gatccgaacc cacttgtttc
     5101 tggcaaagga gcggcacagc ttagagccgc cggcattgag gttgtggaag actttatgcg
     5161 aacggagtgc gatgcgctga acccgatttt cttccattac attcaaacca aacgccctta
     5221 tgtgttgctg aaatatggga tgacggcaga tggtaaaatt gcgacttata cggggaattc
     5281 gaaatggatc accggcgagg ctgcccgagc taatgtacag aaaagccgcc acgaatatag
     5341 tgcgattatg gtcggggtgg aaacggtgct tgccgatgac ccaatgctca acagccgaat
     5401 ggaaaatgct aaacagccgg tgcggattgt atgtgacagc caactgcgta ccccattgga
     5461 tagccaacta gtccaaactg cacgccaata tcgcaccatt attgctacgc tcaataatga
     5521 tgcaaaaaag caggcagaat ataccgcttg cggtgttgaa attttagtga caggcgaacg
     5581 tgacaagcgg gtggatttag ccgatctttt gcaaaagctg ggcgaactcg ggatcgacag
     5641 cttattgatt gaaggtggtt cgcaactcaa tttcagcgtg ctggaacagg ggttagtcaa
     5701 tcgagtacat tgctatattg ccccgaaact gattggcggc aaagcggcaa aaaccccgat
     5761 tggtggcaag gggattggcg agttagcaca agcggttaaa ttacgtctaa aatccaccga
     5821 attgctcgga gaagatattt tgttggatta tgaagtggac ttgcttcgta attaaattga
     5881 aatttcctta ctctcgcccg tttacgggag agagacagca taaaatttcg ttcagaaatt
     5941 ttatgcagag agaggggaaa atgagtgtaa tcttccctct ccctccgaaa atcctacgga
     6001 ttttctcttc cctctcccgt aaacgggaga ggggaatagt aagataagca gttctcagac
     6061 taaattggaa gtaaatcgga gaaaaattta aatgttcaca ggcattatcg aagaagttgg
     6121 ccagatagcc caaattaaga aacaaggcga gtttgtggtg gtaacggtta aagcaaaaaa
     6181 gattctgacc gatgtacatt taggggatag cattgcggta aatggcgtgt gtttaaccgt
     6241 gacttcattt acgcaggagc aatttactgc tgatgttatg tcagaaacct taaaacgtac
     6301 atcacttggc gaattaagta tcaacagctc ggttaatctt gagcgggcaa tggcggcaaa
     6361 cgggcgtttt ggtgggcata ttgtgtcagg tcatattgat ggcacgggtg tggttgcaga
     6421 gattacacca gcagataatt caacttggta tcgcattaaa gccgaaccta agttaatgcg
     6481 ttacatcatt gaaaaaggct caattactat tgatggcatt agtttaacgg tggtggatgt
     6541 tgatgcggaa tctttccggg tatcgattat tccgcatacc attaaggaaa ccaacctcgg
     6601 tacgaaaaaa atcggtagtt tggtgaattt agaaaatgac attgtcggca aatatatcga
     6661 acagttttta ttgaaaaaag agcctgaaaa tccgcaaagt aaaattactg cggactttct
     6721 taaaaatgca gggttttaac caaataaagg acaaataatg tttaagtttt caacggtaga
     6781 agaagcaatt caagcaattg cacaagggaa aattatttta gtcagcgatg atgccgaccg
     6841 tgaaaatgaa ggggacttca tttgtgcagc ggaatttgcg acaccggaaa atatcaactt
     6901 tatggcgaaa tatggtaaag ggttgatttg tacgccgatt tcgacagaaa ttgccaaaaa
     6961 attaaatttt cacccgatgg tgctagagaa cgaagataat catcaaaccg catttaccgt
     7021 ttctgttgac catattcaaa ccggcaccgg tatttccgct tttgagcgtt cattaacttg
     7081 cctgaaaatg cttgatgaaa atgctaagcc ggaagatttt agacgtccgg ggcatgtttt
     7141 cccattagtg gcaaaagacg gaggcgtatt agtgcgtaac ggtcataccg aagcaactgt
     7201 ggatttagct cgtttggcgg gcttgaaacc ggcagggctt tgctgtgaaa ttatggcaga
     7261 agacggcacg atgatgcaga tgccggaatt acaggcattt gcacaagcac acaatatgcc
     7321 gtttattact attcagcagt tacaagagta tcgtagaaaa cacgacagct tggtgaatgt
     7381 ggtttcggtg gtaaaaatgc cgaccaaata cggtgagttc aaagcacaca gtttcgttga
     7441 aactatttca ggcaaagaac acgtcgcttt agttaaaggt gatattggtg atggcgaaaa
     7501 tgtattatgc cgtatccatt ccgaatgttt aaccggcgat gcctttggct cacaacgttg
     7561 cgattgtggt cagcagttcg cagcggcaat gaaccaagtg gagcaagagg gcagaggcgt
     7621 ggtgctttat ttacgccaag aaggacgtgg catcggttta attaataaac tgcgtgctta
     7681 cgaattgcaa gatagaggaa tggacacggt tgaagccaac ctggcattag gtttcaaaga
     7741 tgatgagcgt gaatattata tcgccgctca aatgtttgaa aaattaggcg taaaatcgat
     7801 tcgtttgtta accaataatc cggcaaaaat tgaaggactc attgagcaag gtttaaacgt
     7861 agtggctcgt gagccgatta tggttgagcc aaacgaacac gatttggaat accttaaagt
     7921 taaacagcac aaaatgcgac atatgtttaa tttctaatcc aatactctct ccccttgtgg
     7981 gagagagaca gaaaaatact tcgttcagaa gtatttttca gagagagggt atatttttta
     8041 gaaaaagttt gcaatttccc ctctccctga tttttcttct gaacgaagaa aaatctatcc
     8101 ctctcccaca aggggagagg gttattgagt atcaaattta ttttaaagaa ggaaaacaaa
     8161 caatggcaac atttcaagga aactacatcg caactggttt aaaattcggt attgtggcaa
     8221 ctaactggca caagattttt atcgatcaac tgttaaacgg tgcaaccgat aaattattgc
     8281 gtcacggcgt agaagaaaaa aatatcgaca ccgtatgggt gccgggtgca ttagaaatct
     8341 caattgcagc gaaaaaaatg gcacaaagcg gtcgttatga tgcgattatc tgcttaggtg
     8401 cggtcgtgcg tggagcaacc agccactatg atgtggtagt gaacgagtca gcaaaaggca
     8461 tcagctcttc tgctttagaa accggcgtgc cgattattaa cggtatttta acggtggaaa
     8521 atttagagca agcgattgag cgttcaggca ctaaagccgg taataaaggc gaagagtgtg
     8581 caatggtagc gattgaaatg gcgaacctat taaaagcctt agcgtaatca gcgataaaat
     8641 ccccttagac ccaagagcct aaggggattt ttttattttc tgcgtagtaa caggctggca
     8701 atgacacaaa ataggctgaa actgtaaatt aaccagtgac ctaattgttt aaacggcgta
     8761 tcgcctttgg ttggcacaat ggtggcagtt agtgcatcag cagtaaattg cggtagttgt
     8821 tcaattactt ttccgttggc atcaacaatg gcggtaatac cggtattcgc agctcgaagc
     8881 aatggtttgc caagttctaa cgctcgcatt cttgccattt ggaaatgttg ccaaggcccg
     8941 atggaagaac caaaccacgc atcattggtg atagttaata aataatctga attttggctt
     9001 ttttggtttt gttgtaactg atcaccaaaa ataatttcat agcaaatcgc catattaaat
     9061 cggttgtttt tcgccaagaa tgatggttga acgaaactgc cttgtgaaag attaattggt
     9121 agaataaaca cttcccgcat ccaatccagc accgagccaa acggcacata ttcgccaaac
     9181 ggcacaaggt gatgtttgtt gtagcgaggg ttgctgtgca gggcatattc cctctctttt
     9241 tcgcctaata gcacgctact gttaaacagt tcctcttgct cattttgata gagtgtgccg
     9301 ataataattt cacttccgtg ttggcgagct aagtgatcga gcgagccaag cagcgggctg
     9361 atttgatttt caagtgccgg aatggcggat tcaggcaaaa taatcagttc actgttacct
     9421 aataggggca gaatgagctg ttcgtaggtt cgtacgctgt ggttgaaatg ttgcggatcc
     9481 cacttcattt tttgctcaat attcccttgt actaaactca ctacaagcgg ttgtttttct
     9541 gtatcaattt gcacaaattg gagaaagcga gtggcaaaac ttaagccaaa aatgaccgct
     9601 agtaccatca atgatggcac agttttgttc tcttgctgaa taattttcac tagatagcta
     9661 cttgcaacta ttaccaagaa agtcaaacct tcaacgccca aaatcggcgc aataccagca
     9721 aaggggctgt ctattaagct atagccgaat tgtagccacg gaaaaccggt aaataccacg
     9781 cctcgtaaat attcggtaaa ggtaaaaatc gcagccaatg caaacggatt attcagtttg
     9841 agcctgtgtg aaactacact aaacagcaga ttataaatag cgagatagca cgccaacagg
     9901 aatactgcta tgtagcttac tatggtagga acaccaccaa attgggtcat actcacagaa
     9961 acccagttta caccggtgca gaagtagccg atagaccaag caaaggttgc ccataatgcg
    10021 gtttttctgt ttgccagagt ggctgaccaa accaagccgg ctgcggaagc aaaggcaaga
    10081 atccaaatat caaaaggcga ataggcgaaa gtgccgattg cacccagtat gattgcaagg
    10141 agacaagcgg ttaaaaaagg cggatttttt gcaaaattca taataactca ctaaataaat
    10201 gaggcacgcc gaaacgtgcc ttgattgaaa ctcactatat tattcttctt cgttaggaga
    10261 ggttttttcc attttctcta actgttcatc ggaaagtgtg actcgtagct gaatcaattg
    10321 gcggctatta gctgaggtga ctttaaaatc aattccgttt aactcaattt gctcatctcg
    10381 tttcgggaaa taaccgaaag cctgcatcac taaaccacca atggtatcca cctcttcgtc
    10441 attaaagtcg gtagcaaaaa attcattgaa ttttttgatg tcggtgagcg gtaataccgc
    10501 ataagtatgt cttgaaagct ggcgaattgg tttgacctct tcttcatcaa actcatcttc
    10561 aatatcaccc acaatttgtt caagaatgtc ttcaatggtg actaagcctg aaaccgcacc
    10621 aaattcatca accactactg ccatatggaa gcgttctgaa cggaactctt ttagcattcg
    10681 gtctaggcgt ttgctttccg gtacaataac cgcagggcgt aatacctgtt gcagattaaa
    10741 gggttcagag tcagaacgta aatatttcaa taaatcttta gagtgaagaa taccttcgat
    10801 agtatctttc tcaccccgaa tcaccggaaa acgagagtgt gcggattcaa taattaaatc
    10861 aatacagaca tctaaaggtt cattggcatc aataaacaca atttgcgcac ggggaatcat
    10921 aatatcccgc actcgtaatt ccgagatttc cattactcct tcgatcatct ctttggtgtc
    10981 gctgtcgatc aattcatttt caacagagtc tcgaatcact tcgactagat cttcacggtt
    11041 tttaggctcg ttttgaaaaa gcccgctaaa tagagagtgt aaaatcgatt ttttttctga
    11101 tttttcggtg gtgtttacac tttgctgttc atcactcata gtttgttcca cttaaataaa
    11161 gtacgcttat aataagcaaa ttcagtcaca aagcaaataa aacattgcta ttttcactca
    11221 aattagcata ggataagcaa aaatcttttt atttcttcat attttaggag tttgaaatga
    11281 aattaaccaa aatagcatta agtttaacag ccttagttgt cagttcgatg agtgtggccg
    11341 ggacgttaag tacatcatca atggtggaca ttttagcctt tgacgggcaa cgtgttaaat
    11401 ccggcacgac ttcggttcaa atgagtgaga ataaaacgca tcaagtggta gtggaagtcg
    11461 gtgggacagt tgaaggggat tttttcacct ctgatcaaat cattttgact ttccaaggca
    11521 gtgcggaaaa tgcaaaatta gaaacgccaa gattaaattc aaagttagat cttcgtaaat
    11581 tccaagagaa tcagactgtg gcaattaaaa ccgattcagg taaggttatt tcacataaac
    11641 aagatttctt aaaaggggaa ggtttccttg cgaaaacaag agttgaagat aatttaacga
    11701 aatataactt agcgaaaaaa acagcttcag ttgaacagtt tgcgacggca cctcttgaag
    11761 caaaaggaca aattgtggtg gatacaaata aagtttcaca agaagaatta cagcttttat
    11821 ttagaaaggc agataaagac acccaaaaac gtttcttaga atgggcgaag aaaaatataa
    11881 aataaaccta tatcaattaa taagcggtca tttctcgcca attttttgca aggtgttaaa
    11941 aaaatgaccg ctcatacaat gagatttacc agcaataata gcagtaacgc cagcctctat
    12001 acatatagtc tctatcgtaa ttccaacgaa tttcttcaag ttctacttgt gccttgtaat
    12061 tttgccaagc aagcttataa caggcttgga acattggggc attgactttt tcggcatagc
    12121 gtttcttaaa tgctttctct tcttgttcgg tttggctaag cggcggatta aattgttggt
    12181 acattaactc cgccgtttct acatcacatt gtttggcgag ctgaacttgc aaatcttgtt
    12241 ctgcccgaat ttgcttcgct ttacgttcag cttgttgctc tggagttaaa gtacagctac
    12301 ttaacataag tgcgagtgaa aaaatgcagc ctaatttgag tgttttcatt gctgaatcct
    12361 ctagttgaaa ttggtgattt attctaccgc ttctaactca ctttcttcaa gttcatcagc
    12421 catagcacta agggcatcac ggctaagttg tgcgagggct ttataaaaac cattgtcgaa
    12481 agcttctatt tttcctaaga atttgcctgc acaaggtagt aaaaattgtt cgaataatat
    12541 tttttgagct aaggcagaat cgagattatc ttcgatccaa gatgccgtga gaaatagtaa
    12601 cccaatgtga tcggcatttt ctaattcagg cattgcccgc tcttgtctaa aagctacaaa
    12661 attattaact gataatccat aggctgaaat tgttggagaa atagccccat tttcagcaaa
    12721 aagcgcttga tagcttgctg ctaattgcgt agggttagca ttcttttgta agagagtcag
    12781 cgcattttca ctttggctat ccgtatttaa tgcccagact tggcgtaatc ctccttgggc
    12841 aagccagtta aatgtgccag ctaaaatagg gtcggtaggg tgacgataaa ataagttgcc
    12901 aaacaaacgg caaagcaggg aaaaatcgtt aatggtagtt tcgttcataa tttgaaatag
    12961 atagaaaaaa gaattgttag tatagcagaa cacaagagag gactgaatga aatatatagg
    13021 cgcacacgtt agtgcttcag gtggggtgga aaatgcagtt ttgcgttccg tagaaattgg
    13081 tgctaatgca tttgcgttat ttactaagaa tcaacgtcaa tggcaggcac cggcattaaa
    13141 agccgaaact atcgaaaaat ttaagcgttt ttgtaaagtg catcattttt cagcagagca
    13201 aatcttacca cacgatagct atttgattaa tttaggaaat ccggaagcgg aggcattgga
    13261 gaaatctcgt gtagcattta ttgatgagat gtctcgagca aatcaattag gtttaaaatt
    13321 gttgaatttt cacccggggg cgcatttaaa gcaaatttcc gagcaagatt gtttggctag
    13381 aattgcagaa tcgatcaata ttgcagttga aaaagtaccg aatgttattg ccgtgattga
    13441 aaatacggca ggacagggtt ctaatttagg ttggcatttc gatcatttgg caactattat
    13501 tgagcaagtt gaggataaaa gccgagtggg ggtgtgttta gatacttgtc acttattttc
    13561 tgccggctat gatattagtt cttatgaaaa gagcgaggca gtatttaatg attttaacca
    13621 aacggttggt tttcattatt taaggggaat gcacttaaat ggttcaaaaa caccattagg
    13681 cagcagagtg gatcgtcatc ataccttaag agaaggcaca ataggaaccg aagtgtttgc
    13741 atttattatg caaaatatgc attttaacca tattcctatg attttagaga caatttcgcc
    13801 tgaaatttgg gcagatgaaa taaaatttct acgcacattg gaactaaaat aggttatact
    13861 ggtctgagta gtgggtctag cagagtctag accttatttt gttatctttt ttaatctttt
    13921 agaagattta gttaaggaat ttaccgctgt aattgttctg gcgagacgag acataaatta
    13981 tgaaaaaaaa tctttttgca acaaccttca agcccgtatt tattgcttct attttgtctg
    14041 tttttggagg gtattcttcc attgctgtag caagtttaag ttctaaaccg gccacaccaa
    14101 atcaaatcat tgcaagtaaa gcagaaagat tagctaagtc ttcttcagta tttaccaatc
    14161 gagcaaatct tccaacttct tccgctcata cagcgaatta tagtggtcgt attaatagtg
    14221 tttatcataa ttgggtaggg acacgctatc gtttaggcgg cacaacaaaa gcaggaattg
    14281 attgttccgc ctttgtgcgt gaagtaatgg gcaaagcatt tagtaaagat atgcctcgtt
    14341 caactgcaga acaacgccat gtcgggcatt ccatttcaaa atctgattta cgcccaggtg
    14401 atttagtctt tttccgtaag aataaccatg taggggttta tattggtaat ggtaaatttg
    14461 tacacgctag ttcaagtaga ggggtaacca ccagctcatt gtcagaaagc tactggtcaa
    14521 gaaactacac acaatctcgc cgtgtaattt aataaattac aattccaaga tgaaaagaac
    14581 actcactttt gcgagtgttc ttttttaatt aaaaaagttt atgctacgcg ttgcttagct
    14641 tgtgtaaatg ctccacgagt acgcatttga tgaaccacat tagtaattac acctgctatt
    14701 tttacttcag cccaatcagt aattctaaga tttgattcgc tcacatctaa tgaaaataaa
    14761 attttttcag caataccttc cccattctga atttcattaa agaattgata gaatttatat
    14821 tccccatctt tttcaagcac caatagatca ttaactaaat attggttggt ctgttctaca
    14881 atcaataaat cgtctagttc aattccccac gcgattaaat ttgggttttt cacgtggata
    14941 aaaaaagtgt ctcttgggcg tttgatacaa agtgagttaa gatcgagctt tacagagcgg
    15001 ctgctattga aatcccgata aagcggaatc ggctgataag ctgccgtttg atctgaaatt
    15061 tgtctaattg aagccatagt aaaatcctct atctgttttt ttatacaggg tattttactg
    15121 gctaaaatac cagtgtcaac cattattttg gaattttttg taaatttaac cagtgttttg
    15181 accgcttgtt atcctttaaa aagcaataaa aaagcaccca tagggtgctt cttatgctta
    15241 aaagctgtta ttataggctt tcagtgaatg tacgagtaat aacatcgcgt tgttgctcag
    15301 gggttaatga gttgaaacga actgcataac ctgaaacacg gatcgttaat tgaggatatt
    15361 tctcagggtt ttgaatcgca tcttctaatg tttcacggcg taaaacgttt acgtttaagt
    15421 gctgaccacc ttcaacttta atcgttggtg caacattaac gggtacttca cggtatgcga
    15481 tatcacctaa ttcgctgata gcaacaactt gatcttcttc aaatcctgct tttgcagcta
    15541 aacaacgagc ttcattttta gcttcgtcta ataaccagaa agagtttaat aagttgctgt
    15601 ttgatgattc tg
//
LOCUS       DANLZC010000051        15420 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.051, whole genome shotgun sequence.
ACCESSION   DANLZC010000051 DANLZC010000000
VERSION     DANLZC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15420
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.051"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            39..3197
                     /locus_tag="PXE44_001204"
     CDS             39..3197
                     /locus_tag="PXE44_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL5588720.1"
                     /translation="MITINENTIEQSAIATLQSLGWDYTYGKKILAGLEHEWRERTAE
                     VILKPLLAQAIAKFNPNLPACEVENVVAQVCRADSGDLAERNRQAYDWLRNGVKITYQ
                     LYGEQVSDVVQLIDFQRPENNDFRIVNQLDISGKKGKRIPDLIGFVNGLPLVVFELKN
                     PLKENADIGKAFAQLQTYKDEISDLFVFNQALVISDGIVARIGSLTADFDRFTPWRVV
                     DEKNQSKRIVFEDELTALLQGVMTPKNLLDYVQNFVVFERDGKNRLIKKIGAYHQFYG
                     VNEAVDCTLLAATGNRKIGVFWHTQGSGKSLSMLFYAGKVLSQSSLKNPTLVLVTDRN
                     DLDGQLYATFCGGEALLKQTPIQADGRDELRSALASRSAGGVIFTTIQKFGLMEGELA
                     HPVLNERENIIVITDEAHRSQYGFSQKINHKGEYREGYAKHLRSALPNASFIGFTGTP
                     IELDDKDTQEVFGKYVSIYDFEDAVEDGATVPIIYEPRQISLGESGEFSKVMEEAQQL
                     IDDDENSYNFRLREKLHSVDSRLQKMAEDIIAHYDERTKQQDGKAMVVVMSRAICVKL
                     YEKITALRPEWHSNDVLQGSIKIVMTSNASDPAEWQKHNQDKKTLEKRFKDPDDPLNI
                     VIVRDMWLTGFDAPCCNTMYIDKPMSGHNLMQAIARVNRVFRNKSRENGGLIVDYVGL
                     TDELEKAMKQYTNAGGKEKPVRDISAVLEKMVEHITVIRGQFATPIDGQAVDIAKMLQ
                     ISEPPKLLNAILTSANHILALDRIQPPDNTAKDKTPRKNAFLQSVRLAKKGYALCGAL
                     KAVEPYKQELAFYDAVRATIIKNSTAPRNSSSENDRLLQLTALMNRAVQSDGVVDLFD
                     LLKKDRPNINLLSDEFLETVKNSPTKDLWLSAMERYLASQLREQSGANLATKKAFEQK
                     LKEAMNQYHNHNLSVLEILEELIALAKEFEARQKRGEALGLSPAEMAFYDALARNESA
                     VREMGDEVLMKLAKDITDKLRKSVTVDWQYKDSVRAKMRTLIRIALRTYKYPPDLQAE
                     AIEFVLQQAEEIAGEFAITETA"
     gene            complement(3231..6122)
                     /gene="glnE"
                     /locus_tag="PXE44_001205"
     CDS             complement(3231..6122)
                     /gene="glnE"
                     /locus_tag="PXE44_001205"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDL5588721.1"
                     /translation="MLTTLIPLSQLNLPPNLQNSEQIQPLVTAISLSNFVHQTLQKQP
                     DLLAEWLAKFPTPDDCCNYTERLAKILARVENEEMLGRVLRQFRHRELARLSFIQSNK
                     LATVELVFQHLSDLAESLILAARDWLFQRCCAEYGTPKNTLGETQELLILGMGKLGGR
                     ELNFSSDIDLIFTYPDIGETEGGRKSIENSKFFTRMGQRLIKVLDEITADGFVYRTDM
                     RLRPFGDSGALVLSFTAMEDYYQEQGRDWERYAMIKAKILGEDSQNLNHRYLKQMLRP
                     FVYRRYLDFSAIQSLREMKEKISREVVRRNLTDNIKFGAGGIREVEFIAQTFQMIRGG
                     RDKILQERSLLKVLPRLAELNLLTQTQVETLHNAYIFYRQIENVLQAIDDKQTQTLPT
                     DEASQARLVFACQSYYQQDLYSEKTHWVEHSFNDWQQFMAVLSQYQQAVRQIFNEIIG
                     EEETQANCNPVNEKLAEWKDILHYNIRLEDLSAVLKGYPKVAENDYTEIFRHFSTTFQ
                     DWVKRPIGVRGREVLRNLMPRIADSIFKGEDHLLLLPRVLNIVDKITTRTTYLELMLE
                     KEQILPQLLALCSKSVMIAEQIARYPMLLDELMSHRGLTDVQAFEKYQSALQDYLIRI
                     PEEDEEALIDGLRQFKQTQILRIAAADILGVLLVMKISDHLTYLAEAIINAVVNMAWK
                     QVSQRFGVPEHLEADEKGFAVIGYGKLGGIELGYNSDLDLVFLHNAPEDSQTVGGKKE
                     ISSHQFYLKLAQKINSIFNLNTSAGVLYEVDMRLRPSGEAGLLVSTFNAYEHYQKNEA
                     WTWESQALVRTRCVFGAENLKQAFEKIRQSTLAQPRASGQLRQEICEMRQKMYQHLSS
                     HSAEQFHIKQDQGGITDIEFIAQYLVLAHSHQHPKMAVWSDNVRIFDSAVECGILSSE
                     QSEQLQHCYTALRNKIHHLNLLRKDSVVDASEFTTERAFVREMWQRLLA"
     gene            complement(6294..6575)
                     /locus_tag="PXE44_001206"
     CDS             complement(6294..6575)
                     /locus_tag="PXE44_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDL5588722.1"
                     /translation="MYYVIFAQDNPNMLEKRLEVRPAHLARLEKLQAEGRLLIAGPNP
                     TEDGSSVTGSTVIAEFNSLEEAQQWASQDPYVEAGVYSDVIIKPFKRVF"
     gene            complement(6577..7041)
                     /gene="yciA"
                     /locus_tag="PXE44_001207"
     CDS             complement(6577..7041)
                     /gene="yciA"
                     /locus_tag="PXE44_001207"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDL5588723.1"
                     /translation="MKDKPYGKAPEGKLILRTLAMPSDTNANGDIFGGWIMSQMDLGG
                     AILAKELAKGRVVTVTVDKMIFHRPISVGDVVCCYGKLMHIGRSSMQVKVEVFIKQVY
                     EGNRERYLVTEALFTYVAIDKEGKPRAVPRENNPELDEALSLIDLTPEKQGE"
     gene            complement(7031..7588)
                     /locus_tag="PXE44_001208"
     CDS             complement(7031..7588)
                     /locus_tag="PXE44_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDL5588724.1"
                     /translation="MKQLLEFIPLILFFVVYKMSGIQMASIALVIATIVQMIALKMLY
                     GKIEKQQIIMGGSVVFFGLLSAYFNEVKYLQWKVTIVYALFALVLLVSQFVFKTPLIK
                     KLLGKEIQLPEAVWNKINFGWAVFFLICMLLNIYISQYLSEEIWVDFKSFGIIGLTLV
                     ATVITGFVLYPYLKDAEKQEQKNER"
     gene            complement(7597..8406)
                     /locus_tag="PXE44_001209"
     CDS             complement(7597..8406)
                     /locus_tag="PXE44_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5588725.1"
                     /translation="MAQALLYFTTKYLQSIEGYFMSIKFVDLLQQSWNFMRNQQAFSL
                     FAIVTIVVVQLVFILVSPNSPELMAESQPQQLQIDASKMVSILLPTILLGVVNLLINV
                     LMIFNIQSINDGNYRQFFQNAGNALKHFLPVLLLQFVMVLPLSLGASFAMASPETVII
                     ALPVLVVGFYFFIKLSLVIYAYLLEKPQKSLSESIKFTLQLSRGKMLPLILFCVISYL
                     LPGMLSRLFTVFGNDFIGIFITIVLSAVINVFMAIFSFRFYQVYRQMPAVR"
     gene            8477..8914
                     /locus_tag="PXE44_001210"
     CDS             8477..8914
                     /locus_tag="PXE44_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDL5588726.1"
                     /translation="MLSLFDAGQRYLNTLPNQKKLANFMPDYHIIRLVKFSSKVMPAF
                     ACFALVWQYCFTDPTQSILANSVLTAVFAISIPFQGLYWLGKRARSPLSLSLLEWYES
                     LRQKLISAQVKLEETQAVPSFQDFANLLKLAEETWGDEYFDEL"
     gene            complement(9034..9109)
                     /locus_tag="PXE44_001211"
     tRNA            complement(9034..9109)
                     /locus_tag="PXE44_001211"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9074..9076),aa:Asn,seq:gtt)
     gene            complement(9225..9974)
                     /gene="moeB"
                     /locus_tag="PXE44_001212"
     CDS             complement(9225..9974)
                     /gene="moeB"
                     /locus_tag="PXE44_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDL5588727.1"
                     /translation="MELTDHEMLRYNRQIILKSVDFDGQEKLKASRVLIVGLGGLGCA
                     ASQYLASAGVGHLTLVDFDLVSLSNLQRQILHTDATIGQPKVESAKARLAAINPNIQI
                     ETINQRCSDEQLAKLIANVDLVLDCTDNVTVRNQLNLQCFKQKRPLVSGSAIRFEGQV
                     SVFCYQEQEPCYECLSRLFGDATLSCVEAGVIAPIVGIIGSIQALEAIKVLLNIGRTL
                     SGKLLMIDGLHFSVREMALPKQNNCPVCTSY"
     gene            complement(10073..11302)
                     /gene="moeA"
                     /locus_tag="PXE44_001213"
     CDS             complement(10073..11302)
                     /gene="moeA"
                     /locus_tag="PXE44_001213"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDL5588728.1"
                     /translation="MALLALSDALDNMLARLSTPKLVEQLPLNECANRVLAEDIFSPI
                     NVPNFDNSAMDGYAVRISDLAQNLTLNVAGKAFAGNPFNGEIKAGECVRIMTGALVPK
                     GVDAVVMQEETTLNSDGSVTFNHIPKLGSNIRRIGEDVKQGALVLKKGSLLNVASLPL
                     LASLGIASVPVFVKLKVAILSTGDELTSVGEPLSEGKIYDTNRFAVRLMLEKLNCEIL
                     DFGILPDDPILFEQTFRQAQQQADVLITSGGVSVGEADFTKDVLEKLGEIGFWKIAMK
                     PGKPFAFGKLEQAWFFGLPGNPVSALVTFYQLAQPALAKLSGLSTDKIAKLTQNLTAC
                     SAGKLKKAVGRQDFQRGYYYANEKGELEVRAVGTQGSHIFSAFNESNCFIVLEAERGN
                     VDAGEKVTIQPFNSLMY"
     gene            complement(11324..11941)
                     /locus_tag="PXE44_001214"
     CDS             complement(11324..11941)
                     /locus_tag="PXE44_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HDL5588729.1"
                     /translation="MFDSLAVQFVVLWAVIDPIGSVPVYLAKTIGLPIEARRKIALNA
                     VLIAAGILIFFLIGGQVLLESMQIPLPAFQAAGGLVLLLFALTMIFGESKPEQEMRIS
                     SNLNELAVYPLAVPSIASPGAMMAIVLLTDNHRFHIGDQIITAGIMLLVLFITYLLLL
                     AANRIQHLIGNAGAAIISRVMGLILAAIAVNNLLIGVRDFFVQIS"
     gene            complement(12009..13220)
                     /locus_tag="PXE44_001215"
     CDS             complement(12009..13220)
                     /locus_tag="PXE44_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL5588730.1"
                     /translation="MISRQMQSLLKRLVAQFPAVLVTGARQVGKSTLLQHIGEGYQYI
                     TFDDPMLLELAKQESRLFFLNHQGKLILDEVQYVPEIFALLKLEIDKKQQNGLFLLSG
                     SQAFELMQNVSETLAGRIAILKLQGLSLREILGVKFDQPFLPTTDYLLAREQELKSPE
                     NIWQLIHKGDMPRLYAQEVDWEIYYRSYVATYIERDVRQLTNIASTTDFTRFMVSIAS
                     RSGELLNYSNIAQEVGVSNETIKRWVAILQTSGIIYLLQPYHNNHLKRAIKTPKVYFL
                     DTGLMAYLTKWLTPETIAQGAKSWQFFETFVVSQIIKSFHNQGIEPPIYFYRDTNQKE
                     IDLIIEYDRTLYPIEIKVTASPTKKMAKSFDLLNSVSSEWKLGQGAIINQYPTKLYLS
                     ENLVALPVSYL"
     gene            13417..15348
                     /locus_tag="PXE44_001216"
     CDS             13417..15348
                     /locus_tag="PXE44_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5588731.1"
                     /translation="MIFFTDLILKRGHSVLIDNSSVTIHTGQKVGLVGKNGCGKSSLF
                     ALLKNELQPEGGDVSLPKNWAISWVNQETPALAISALDYVIQGDREYTHLMAELDIAN
                     EKNDGNRIAIIHTQLDTIDAWTIQSRAATLLNGLGFSTEQLQLPVKSFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLERWLSNYRGTLLLISHDRDFLDPIIDRVIH
                     IEHQKLNEYTGNYTSFEIQRATKIAQQNAAYQQQQRKVAHLQSFIDRFKAKATKAKQA
                     QSRIKALEKMELIAPAYADSPFEFEFRPPLALPSPLLMMEKVSAGYAEKTVLESVKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELPPQNGSIQLAKGVQLGYFAQHQVDTLRFDE
                     SPLWHLQKLAPEKTEQELRNYLGGFDFKGDKVVQAVKSFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLEMRQALVDALTYYEGSLVVVSHDRHLLRSTVNEFYLVHDRKVEKF
                     KGDLDDYQKWLNEQNALANEPKSVLAKDSANSTACSSTNENSAAQRKEQKRLEAERRQ
                     QTAPLRKKITQLEKELEKATEKLNKLEAMLTESDIYEAENKAKLTETLAKQLEAKRLV
                     EEIEMQWLEAQEEFETMLR"
BASE COUNT         4636 a         3384 c         3162 g         4238 t
ORIGIN      
        1 ttattaccta aattattaag tggggagatg gagttgtgat gattaccatc aacgaaaaca
       61 ccattgaaca atccgccatt gcaactttgc aaagcttggg ctgggactac acctacggca
      121 aaaaaatttt ggcaggttta gaacacgaat ggcgggagcg aaccgctgag gtgattttaa
      181 agccgctttt ggcgcaagcg attgcaaaat tcaacccgaa tttgcccgct tgtgaggtgg
      241 aaaatgtggt ggcacaggtg tgccgtgccg acagtggcga tttggcggag cgtaatcgtc
      301 aggcttatga ttggctaaga aatggggtca aaatcactta tcagttgtac ggtgagcagg
      361 tgtctgatgt ggtgcagctg attgattttc agcgtccaga aaataacgat tttcgcattg
      421 tcaatcagct ggatattagc ggcaaaaaag gcaaacgcat tccggatttg attggctttg
      481 ttaatggctt gccgctggtg gtgtttgagc tgaaaaatcc gctcaaagaa aatgccgaca
      541 ttggcaaggc gtttgcccaa ctgcaaacct ataaagatga aatttctgat ttgtttgtgt
      601 ttaatcaggc actcgtgatt tcagacggca ttgtcgcccg catcggttcg ctgaccgctg
      661 atttcgaccg ttttacccct tggcgtgtgg ttgatgaaaa aaatcagagc aaacgcattg
      721 tgtttgaaga tgaactcacc gccctgctgc aaggcgtgat gacacccaaa aatctcttgg
      781 attatgtgca gaattttgtg gtgtttgaac gggacggcaa aaaccgctta attaagaaaa
      841 tcggagcata tcatcagttt tatggcgtga atgaagcggt ggattgcacc ttgcttgccg
      901 ccacaggcaa ccgcaaaatc ggcgtgtttt ggcatacgca aggttcgggc aaatcgcttt
      961 cgatgctgtt ttatgcaggt aaagtattaa gccaaagcag cctgaaaaat ccaactttgg
     1021 tgttggtaac cgaccgcaac gatttggacg gtcagcttta cgccactttt tgcggtggcg
     1081 aggcactgct caaacaaacg ccaatccaag ccgatgggcg agacgaactc cgctctgccc
     1141 ttgccagtcg ttcggcaggc ggtgtgattt ttaccaccat tcaaaaattc ggcttaatgg
     1201 agggtgagct tgcccaccct gtattaaacg agcgggaaaa catcattgtg attaccgatg
     1261 aagcacaccg ctcacaatat ggttttagcc aaaaaatcaa ccacaaaggc gagtatcgtg
     1321 agggttatgc caagcattta cgcagtgcat tgccgaatgc ctcttttatt ggctttactg
     1381 gcacaccgat tgaacttgat gacaaagaca cccaagaagt cttcggtaaa tatgtctcga
     1441 tttatgattt tgaagatgcg gtggaagatg gggcaaccgt gccgattatt tatgagccac
     1501 gccaaatcag cttaggcgaa agtggcgagt tttccaaagt gatggaagag gcacaacaac
     1561 tgattgatga cgatgaaaac agctataact tccgcctgcg tgaaaaactg cacagcgtgg
     1621 atagccgttt gcaaaaaatg gcggaagata ttattgccca ttacgatgag cgtaccaaac
     1681 agcaagacgg caaggcgatg gttgtggtga tgagccgtgc catttgcgtg aaattgtacg
     1741 aaaaaatcac cgcacttcgt ccagaatggc actcaaacga tgtgcttcaa ggcagcatta
     1801 aaattgtgat gacaagtaat gccagcgacc ctgccgagtg gcaaaaacac aatcaggata
     1861 agaaaacctt agaaaaacgc tttaaagacc cagacgatcc gctcaacatc gtgattgtgc
     1921 gggatatgtg gctgacaggc tttgatgcgc cctgctgtaa cacaatgtac attgataaac
     1981 caatgagcgg acacaacctg atgcaggcaa tcgctcgggt aaatcgggta tttcgcaaca
     2041 aaagccgtga aaatggcggc ttgattgtgg attatgtggg cttgaccgat gagctggaaa
     2101 aagcgatgaa gcagtacacc aacgcaggcg gtaaagaaaa gccggtgcgg gacatttcag
     2161 cggtgctgga aaaaatggtg gaacatatca cggtcattcg tgggcaattt gccacaccga
     2221 ttgacggaca agcggttgat attgccaaaa tgttgcaaat cagcgaaccg cctaaactgc
     2281 tcaatgcgat tttgacatca gccaaccata ttcttgccct agaccgcatt cagccacctg
     2341 ataacactgc caaagacaaa accccacgca aaaacgcctt tttgcaatcg gtacgcttgg
     2401 cgaaaaaagg ttatgctttg tgtggggcat taaaagcagt tgaaccctat aaacaggaac
     2461 tggcgtttta tgatgcagtg cgtgccacca ttatcaaaaa cagcaccgct cctcgcaatt
     2521 cttcgagcga aaatgaccgc ttgttgcagc ttaccgcctt gatgaatcgt gcggtacagt
     2581 cggatggtgt ggtggattta tttgatttgc tgaaaaaaga ccgcccaaac atcaacctgc
     2641 tttctgatga gtttttggaa acagtaaaaa acagcccaac caaagatttg tggctatcag
     2701 caatggagcg ttatcttgcc tcacaactgc gagagcaaag cggtgccaat cttgccacaa
     2761 aaaaagcatt tgaacagaaa ctcaaagagg cgatgaacca ataccacaac cacaatttaa
     2821 gcgtattgga aattttagaa gagctgatcg ccctcgccaa agagtttgaa gcccgccaaa
     2881 aacgagggga agcgttggga ctaagccctg cggaaatggc gttttatgat gctttggcac
     2941 gtaatgaaag tgcggtgcgg gaaatgggcg atgaggtatt gatgaaactt gccaaagaca
     3001 ttaccgataa attacgcaaa tcggtcaccg tagattggca atataaagac tcggttcgtg
     3061 ccaaaatgcg aaccttaatc cgcattgctc tgcgtaccta taaatacccg cccgatttac
     3121 aggcggaagc gattgagttt gtgttgcaac aagcggaaga gatcgccgga gagttcgcta
     3181 tcaccgaaac ggcgtaatct atattagggc gtgcaatgta cgccctaaat ttatgctaat
     3241 aacctctgcc acatttcccg cacaaatgct cgctcggtgg taaattcact tgcgtccacc
     3301 acgctgtctt tgcgtaataa gtttaggtgg tggattttgt tacgcagtgc ggtgtagcaa
     3361 tgttgtagct gttcgctttg ttcggaactt aaaataccgc attctactgc gctgtcgaaa
     3421 attcggacat tatccgacca tactgccatt ttcgggtgtt ggtggctgtg ggctaatacc
     3481 aaatattggg cgataaattc aatatcggta atacctcctt gatcttgttt aatatgaaat
     3541 tgctcggctg aatgcgagct taaatgttgg tacattttct gtcgcatttc gcaaatttct
     3601 tggcgtaatt gaccgcttgc acgaggctga gcaagcgtgc tttggcggat tttttcaaag
     3661 gcttgtttga ggttttccgc cccaaataca catcttgtcc gcacgagggc ttggctttcc
     3721 cacgtccacg cttcattttt ttgataatgc tcgtaggcat tgaaagtgct gactaaaagc
     3781 ccagcctcgc ctgatgggcg aagtcgcata tccacttcat atagtacgcc ggcactggta
     3841 ttaaggttaa aaatcgagtt aattttttga gccaatttta agtaaaattg atggcttgaa
     3901 atctcttttt taccgccaac agtttggcta tcctctgggg cattatgcaa gaaaaccaag
     3961 tcgagatctg aattatagcc cagttcaatc ccgcctagct tgccgtagcc aataactgca
     4021 aacccttttt catcagcctc taaatgttcc ggcacaccaa agcgttggct gacttgcttc
     4081 cacgccatat ttaccaccgc attaataatc gcttctgcta agtaagtaag atgatcgctg
     4141 atcttcatta ccagcagcac gcctaaaata tcagcagcgg cgatgcgtaa aatttgggtt
     4201 tgtttgaact gacgtaagcc atcaatcagt gcttcttcat cttcttcagg aatgcggatt
     4261 aaataatcct gcaaggcaga ttgatatttt tcaaaagcct gcacgtcggt taagcctcta
     4321 tggctcatca gctcatcaag tagcattgga tatcgagcta tctgttccgc aatcataacc
     4381 gacttactac acaatgctaa aagttgaggc agaatctgct ctttttcgag cattaattcc
     4441 aaataggtag ttcgggtggt gattttatcg acaatattca acacgcgagg caataacaat
     4501 aaatgatctt cacctttaaa aatgctgtcg gcaattctag gcattaaatt gcgtagcacc
     4561 tctcgccctc ttaccccaat cgggcgtttt acccaatctt gaaaggtagt agaaaagtga
     4621 cggaaaatct ccgtataatc attttctgca acctttggat aaccttttag cactgcggat
     4681 aaatcttcaa ggcgaatgtt atagtgcaaa atgtctttcc attcggctaa tttttcatta
     4741 accggattgc agttggcttg tgtttcctct tcaccgataa tttcattaaa aatctggcgt
     4801 actgcttgtt gatattggct caatactgcc ataaattgtt gccaatcgtt aaaggaatgc
     4861 tccacccaat gcgttttttc gctatataga tcttgctgat aatagctttg gcaagcaaat
     4921 acaaggcggg cttgcgaggc ttcatcagtg ggtagggttt gagtttgttt atcgtcaatc
     4981 gcttgtaata cattttcaat ttgacggtaa aaaatatagg cattgtggag cgtttccacc
     5041 tgcgtttggg ttaataaatt taattctgcc aagcgtggta gcacttttaa caggctacgt
     5101 tcttgtaaaa ttttatctcg cccgccacgg atcatttgga aagtttgggc gataaactcc
     5161 acttcccgaa tcccaccggc accaaattta atattatcgg ttaaattacg gcgaaccact
     5221 tcacggctga ttttttcttt catttcacgc aacgattgga ttgcactgaa atccagataa
     5281 cggcggtaaa caaaagggcg gagcatttgc tttaaatagc gatgatttaa attttggcta
     5341 tcctcgccta aaatttttgc tttaatcatc gcataacgtt cccaatcacg cccttgctct
     5401 tggtaataat cttccattgc agtaaaactc agcaccaagg ctccgctatc accaaagggg
     5461 cgaagtcgca tatcggtacg atacacgaaa ccatcagcgg tgatctcatc taatacttta
     5521 attaaccgct gccccattcg ggtaaaaaat ttgctgtttt caatcgattt gcgtccacct
     5581 tcggtttcac caatatcggg gtaggtaaaa atcaggtcaa tatcggaaga aaaattaagc
     5641 tctcgtccgc ccaatttgcc catacctaaa atcagcagtt cttgtgtttc gccaagagtg
     5701 ttctttggcg tgccatattc ggcacagcaa cgctgaaaca accaatctcg agcggctaga
     5761 atcaggctct cggctaaatc cgacaaatgc tgaaatacca attctaccgt cgctaattta
     5821 tttgactgaa taaagctcag acgagccaat tcacggtggc gaaattgccg caaaaccctc
     5881 cccaacattt cttcattttc cactcttgct aatatttttg ctaaacgctc agtataattg
     5941 cagcaatcat caggcgtagg aaacttggca agccattctg ccagtaaatc cggctgtttt
     6001 tgcaaggttt gatgcacgaa attggaaagc gaaatcgccg tcacaagcgg ttgaatttgc
     6061 tcagaatttt gcaaattggg cggtaaattg agttgggata agggaattag ggtggtaagc
     6121 ataaatttag gctcaaaatt gaaaatagat agaattttac gctcgatcaa ttcccctctt
     6181 tagcaaagag ggcttagggg agatttggca gaggtaattt tactctcata cgaaacatta
     6241 atatcgtaat caaatctccc ccagcccctc ttttctaaag aggggagaaa aaattaaaac
     6301 actcttttaa acggtttaat aatcacatcg ctatacacac cggcttccac ataaggatct
     6361 tggcttgccc attgttgagc ttcttcaagt gagttgaact ctgcaattac agttgaaccg
     6421 gttacgcttg agccgtcttc agtcgggtta ggccctgcga ttaataatcg accttctgcc
     6481 tgtaattttt ccaaacgagc caaatgagcc gggcgaactt ctaaacgttt ttccaacata
     6541 ttgggattat cttgggcaaa gataacataa tacatactat tctccttgtt tttcaggggt
     6601 taaatcaatt agcgataatg cttcgtctaa ttcggggtta ttttctctcg gcacagcacg
     6661 cggtttgcct tctttatcaa tcgctacata ggtaaataag gcttcggtta cgagataacg
     6721 ttcgcgattg ccctcataaa cttgtttgat aaagacctcg actttcactt gcatcgaaga
     6781 acgccctatg tgcattaact tgccgtagca gcagaccaca tcaccgactg aaatcgggcg
     6841 gtggaaaatc attttatcta ccgttaccgt aacgactcta ccttttgcca actctttggc
     6901 taaaattgca ccgcctaaat ccatctgcga cataatccaa ccgccgaaaa tatcgccatt
     6961 ggcgttagta tctgacggca ttgccaaagt tcttaaaatc agtttccctt ccggggcttt
     7021 tccatagggt ttatctttca tttttttgtt cctgcttttc agcatctttt aagtaagggt
     7081 aaaggacgaa accggtaata accgttgcga ctaaggttaa gccaataatg ccaaaggatt
     7141 tgaaatcaac ccaaatctct tccgataaat attgactgat ataaatattc agcagcatac
     7201 aaatcagaaa gaatactgcc caaccgaagt ttattttatt ccacactgct tcaggcaact
     7261 gaatttcttt gcctaataat tttttgatta atggtgtttt gaacacaaat tggctcacta
     7321 atagcaccaa tgcaaacaga gcgtaaacaa tggttacttt ccattgtaaa tatttaactt
     7381 cattaaaata ggcactcagt aagccgaaaa agaccaccga gccacccata ataatttgct
     7441 gtttttcaat tttgccgtag agcattttta aggcgatcat ttgcacgatt gtagcgataa
     7501 ccaatgcgat agacgccatc tgaataccgc tcattttata cacgacaaaa aacagaatca
     7561 gcggaataaa ttcaagtaac tgtttcattg atactcctaa cgcactgccg gcatttggcg
     7621 gtaaacttgg taaaagcgga agctgaaaat cgccataaag acgttaatta ccgcacttaa
     7681 aacaatggta atgaatatgc caataaagtc attaccaaac actgtgaaca aacgagataa
     7741 catccccggt aataaataag aaatcacaca aaataaaatc agcggtagca tttttccacg
     7801 tgataattga agcgtaaatt taatactttc cgatagactt ttctgcggtt tctccaataa
     7861 ataagcataa atcactaacg ataatttgat aaaaaaataa aaaccgacta ccaacaccgg
     7921 caatgcaata atcactgttt caggcgatgc cattgcaaaa gatgcaccga gagaaagcgg
     7981 taacaccatc acaaattgta acaacagcac aggtaggaag tgctttaatg cgtttcctgc
     8041 attttggaaa aattgacgat aattgccatc gttaatcgat tggatattga aaatcattaa
     8101 gacattgatc aaaagattga caacacctaa taaaatagtt ggcaatagta tagacaccat
     8161 tttagaggca tcaatttgaa gttgttgcgg ttgcgactcc gccattagct caggcgaatt
     8221 tggcgacact aaaataaaaa ccaactgcac aaccacaatg gtgacaatgg caaacagtga
     8281 aaatgcttgt tgattacgca taaagttcca actttgctgc aataaatcga caaatttaat
     8341 tgacataaaa tacccttcaa tagattgtaa atattttgtt gtgaagtata gcaaggcttg
     8401 agccaaactg cattaaccta ttgtagattt taatcagtta gctaaaataa aagaaaaatt
     8461 tgaaagagat aatcctatgt taagcttatt tgatgcagga cagcgttacc tcaacactct
     8521 gccgaatcag aaaaaattag ccaattttat gccagattac cacattattc gcttggtaaa
     8581 attttcctct aaagtaatgc ctgcttttgc ttgctttgca ttagtttggc aatattgctt
     8641 taccgatccg acccaatcta ttttagccaa ttcggtactt actgctgttt tcgccatttc
     8701 tattccgttt caagggttat attggcttgg aaaacgggca agatcaccgt tatctttatc
     8761 attattggaa tggtatgagt cgcttagaca aaaattaatt tcggcacaag tcaaattaga
     8821 agaaacacaa gcggtacctt ctttccaaga ttttgcaaat ttactcaagc tagcagaaga
     8881 gacttgggga gatgaatatt ttgatgagtt ataaaataaa aaaagcccca cttaaaataa
     8941 gtggggtaaa acttttgttc taattctgga ggtttgaatt actgttttat aaaaggagac
     9001 aaataaaaca agaacaaaag aaaaataaaa tcttggctcc tcctgcggga ctcgaacctg
     9061 cgacatatgg attaacagtc caccgttcta ccgactgaac taaggaggaa ttaacggtgt
     9121 aaataatagg ctaaaacgtt ttagccgtca aataaaaaaa ataaactgtc gttcattttt
     9181 tacatttctt gacatttttt cgccaataag tatgagaaaa atacttaata agaggtacaa
     9241 accggacagt tattttgctt aggtaatgcc atttctctga cagaaaaatg caagccgtca
     9301 atcattaaca attttccgga aagagttctg ccgatattaa gtagcacttt aattgcttcc
     9361 aacgcttgaa tactgcctat gatgccaaca atcggagcaa tgacccccgc ttcaacacaa
     9421 cttaaagtcg catcaccaaa caacctgctt aaacattcat agcacggttc ttgttcttga
     9481 taacaaaata cactcacctg cccttcaaat cgaattgccg aaccggaaac aagcggtcgt
     9541 ttttgcttaa aacattgcaa atttaactga ttacgcacag taacgttatc ggtacaatcc
     9601 agcactaaat ctacatttgc aatcagcttg gctaactgct catcggaaca tcgctgattg
     9661 atagtttcaa tctgaatgtt cggattaatc gccgctaatc gagctttggc agattccacc
     9721 tttggctgcc cgatagtcgc atcggtatgt aaaatttggc gttgcagatt agaaagtgag
     9781 accagatcaa aatccaccaa agtcaaatgc ccaacgccgg cagaagctaa gtattgcgat
     9841 gccgcacagc ccaaaccacc taaaccgaca atcaacactc ggctggcttt taacttttcc
     9901 tgcccgtcaa aatccacgct ttttaggata atctggcgat tataacgtag catttcgtga
     9961 tcggtaagtt ccatttttac acctaaagag taaacgatgg cgcaagcgtc cacgcttgtg
    10021 cctatatttt tattgctagc acaagcgtgg acgcttgcgc tatcttaatt cattaataca
    10081 tcagcgaatt aaacggctga atagtgactt tttccccagc atcaacattg cctcgttctg
    10141 cttctagcac aataaaacag ttactttcat taaaagcgct gaaaatatgt gagccttgcg
    10201 tgccaactgc acgaacttct aactcacctt tttcattggc atagtaatag ccccgttgga
    10261 aatcttggcg accgaccgct tttttcaatt ttccggcact gcaagcggtt agattttggg
    10321 ttaattttgc aattttatct gtcgataaac ccgaaagttt ggctaacgcc ggttgcgcta
    10381 attggtagaa agtcactaat gcagagacag ggttgcccgg cagcccaaaa aaccaagcct
    10441 gttctaattt accgaatgca aaaggcttgc ccggcttcat cgcaattttc cagaaaccga
    10501 tttcgcctaa tttttccagt acatctttgg taaaatctgc ctctcccaca gatacgccgc
    10561 cactggtaat caacacatcg gcttgctgtt gggcttgcct gaaagtttgc tcaaacagaa
    10621 ttgggtcatc aggcaaaatc ccaaaatcta aaatctcgca gttgagtttt tccagcatta
    10681 aacgcaccgc aaaacggttg gtgtcgtaaa tttttccctc actcaaaggc tcgccaactg
    10741 aggttaattc atcgccggtt gaaagaattg ccacttttaa ttttacaaaa acaggcacag
    10801 aagcaatacc gagtgaagct agcaacggta aactggctac attcaataat gagccttttt
    10861 tcaagaccaa tgccccctgt ttaacatctt ccccaatgcg gcgaatgtta ctgcctaatt
    10921 ttggaatatg gttaaaggtt acagagccat cggaatttaa cgtagtttcc tcttgcatta
    10981 caacggcatc aacgcccttt ggcactaatg ctcccgtcat aatccgcacg cattcgcctg
    11041 cctttatttc gccgttaaac gggttaccgg caaaggcttt accagccaca ttcaaggtta
    11101 aattttgtgc taaatctgaa atacgaaccg cgtagccgtc catcgccgaa ttatcaaaat
    11161 tcggtacatt aatcggggaa aaaatatcct ctgccaacac tcggttagca cactcattta
    11221 acggaagttg ttccaccaat ttaggtgtcg ataaacgagc aagcatatta tctaacgcat
    11281 cactcaaagc caaaagtgcc ataatttcct acctattcaa cgtttagcta atttgtacaa
    11341 agaaatcccg tacgccaatc aacagattat tcaccgcaat cgcagccaga attaacccca
    11401 tcactcggct gataatcgca gcccctgcat taccaataag gtgctgaata cggtttgccg
    11461 ctaatagcaa caaataggta ataaagagta ccaaaagcat aatgccggcg gtgataattt
    11521 gatcgccgat atgaaaacgg tgattgtcgg ttaataatac gattgccatc atcgccccgg
    11581 gagaagcaat cgacggtaca gctagcggat aaaccgccaa ttcattcaga ttactgctga
    11641 tccgcatttc ttgctcgggc ttgctttcgc caaaaatcat cgttaaagca aataacaaca
    11701 gcactaagcc gcctgccgct tggaatgcag gtaacggaat ctgcattgat tctaacagca
    11761 cttgcccacc gataaggaag aaaatcaaaa taccggcggc aatcaagacc gcatttagtg
    11821 caattttacg ccgtgcttca atcggcaaac caatggtttt tgccaaataa acaggaacag
    11881 aaccaatcgg gtcgatcacc gcccataaaa ccacaaattg taccgctaat gaatcaaaca
    11941 tattctctcc tgttatcttg ataatttgca aaattttagc ataaaacaac cgcttgcacg
    12001 gctttttctt acagatagct caccggtaac gctaccaaat tttcacttaa ataaagtttg
    12061 gtcggatatt ggttaataat cgctccctgc cctaatttcc attcactaga aactgagttt
    12121 agcaagtcaa aactttttgc catttttttg gttgggcttg ccgtgacctt aatttctatc
    12181 ggataaagcg tgcgatcata ttcaataatt aagtcgatct ctttctggtt agtatcacgg
    12241 taaaaataaa tcggcggctc aattccttga ttatgaaagg attttataat ttggcttacc
    12301 acaaaggttt caaagaactg ccagcttttt gccccttgag caatagtttc gggggtaagc
    12361 catttggtta aatacgccat taacccggtg tctagaaaat acacttttgg ggtcttaatt
    12421 gcccgtttca aatgattgtt gtggtatggc tggagcaaat agataatgcc ggaagtctgc
    12481 aaaatggcga cccaacgttt aatcgtttca ttcgatactc caacttcttg agcaatatta
    12541 ctgtaattga gtagctcacc actacgagag gcaatagaga ccataaaacg ggtaaaatcc
    12601 gttgtgctgg caatattggt taattggcga acatctcgct caatgtaggt cgctacataa
    12661 gagcgataat aaatctccca atccacctct tgtgcatata aacgaggcat atcgccttta
    12721 tggataagct gccagatgtt ctccggtgat tttaattctt gctcccgagc taacaaatag
    12781 tcggttgtcg gtaaaaaagg ttgatcgaac ttcaccccta aaatttcacg caacgaaagc
    12841 ccttgtaatt ttaaaatagc aatacgaccg gcaagtgttt cactcacatt ctgcattaat
    12901 tcaaaggctt gcgaccccga aagtaaaaac aaaccgtttt gctgtttttt atcaatttct
    12961 aactttaata acgcaaaaat ttcaggcaca tactgtactt cgtctaaaat aagcttgcct
    13021 tgatggttta aaaaaaacag gcgagattct tgtttagcca gctccagcaa cattgggtca
    13081 tcaaaagtaa tatattgata gccctcacca atatgttgta acaatgtcga tttaccaacc
    13141 tgtcttgccc ctgtcactaa aacggcagga aattgcgcca cgagtcgttt taacagcgat
    13201 tgcatttgcc gagaaatcat ttttcctcca aaatagccta aatcaagttt aacctcatat
    13261 ataatgttga attaaactta atttaaatta ttttttaagt ttaattcaac acagaatatt
    13321 gaattaaact gaatttctta ttttagttca caaagcaatg gctttgcttt tttatgctaa
    13381 aatacgcaaa atttttaaca aaatagaccg cttgcaatga tcttttttac agacttaatt
    13441 ttaaaacgag ggcattccgt tctcattgat aacagctccg ttaccattca caccgggcag
    13501 aaagtcggtt tggtgggaaa aaacggctgt ggcaaatcct ctctttttgc cttactgaaa
    13561 aatgaattac aacctgaagg tggcgatgta tcgttgccga aaaactgggc gatctcgtgg
    13621 gtaaatcaag aaacgcctgc gttagctatt tcagcgttag actatgtgat tcaaggagat
    13681 cgggaatata cccatcttat ggctgagcta gatatcgcaa atgaaaagaa tgacggcaac
    13741 cgcattgcga ttatccatac tcaacttgat accattgatg cttggacaat tcaatcccgt
    13801 gccgctaccc tgcttaacgg cttggggttt agcaccgaac aactgcaact gccggtgaaa
    13861 tccttctccg gtggctggcg tatgcggtta aatttggcac aagctctgat ttgtcgttcc
    13921 gatctgttat tgcttgacga gccaaccaac cacttagact tagatgcggt tatttggttg
    13981 gagcgttggc tgagtaacta tcgtggcact ttattgttaa tttcgcacga ccgtgatttt
    14041 ctcgatccga ttatcgatcg ggtgattcat attgaacatc aaaagctcaa tgaatacacc
    14101 ggcaattaca cctcgtttga gattcaacgt gcgaccaaaa tcgcccagca gaatgccgct
    14161 tatcagcaac agcaacgtaa agttgctcat ttgcagagtt ttatcgatcg ctttaaagcc
    14221 aaagcgacta aagcgaaaca ggcacaaagc cgaattaaag cgttggaaaa aatggagctt
    14281 attgccccag cctatgctga ttcgccgttt gaatttgaat ttcgcccacc gcttgcactg
    14341 cctagcccgt tattaatgat ggaaaaagtc agtgccggct atgcggaaaa aacggtgttg
    14401 gaatcggtca aactgaattt ggttcccggc tctcgtattg gtttactcgg gagaaacggt
    14461 gcagggaaat ccacgctgat taaattattg gcgggcgaac ttccgcccca aaacggctca
    14521 attcaactgg caaaaggcgt gcaactcggc tatttcgccc aacatcaggt ggatacacta
    14581 cgttttgatg aaagcccact ttggcattta caaaaactcg caccggaaaa aaccgagcag
    14641 gaattacgca attatttagg cggtttcgat tttaaaggcg ataaagtggt acaagctgtg
    14701 aaatcattct ccggcggaga aaaggcccgt ttagtgcttg ccttaattgt atggcaacgc
    14761 ccgaatttac tgctattaga tgagccaacc aaccatttag atttagaaat gcgacaagcc
    14821 ctggttgatg cactgaccta ttacgaaggc tcgctagtgg tggtttcgca cgaccgccat
    14881 ttattgcgta gcaccgtgaa tgaattttat ttagtgcacg accgcaaagt ggaaaaattt
    14941 aaaggtgatt tagacgatta ccaaaaatgg ctcaatgagc aaaacgcctt agccaacgag
    15001 ccgaaatccg tacttgcaaa agattcggca aattcgaccg cttgctctag caccaatgaa
    15061 aatagtgcag ctcaacgcaa agagcaaaaa cgcttagagg cagagcgccg ccaacaaacc
    15121 gccccattgc ggaaaaaaat tactcaactg gaaaaagagt tagaaaaagc aaccgaaaaa
    15181 ttaaataaac tcgaagcaat gcttaccgaa agcgacattt atgaagccga aaataaagca
    15241 aaactaactg aaacacttgc gaaacagtta gaggcaaaaa ggctggttga agaaattgaa
    15301 atgcaatggt tggaggctca ggaggagttt gagaccatgt tacgctaggg ttctacaaga
    15361 tagcgcaagc gtccacgctt gtgccattta agggaactat acacctcaaa aaaacaatga
//
LOCUS       DANLZC010000052        15396 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.052, whole genome shotgun sequence.
ACCESSION   DANLZC010000052 DANLZC010000000
VERSION     DANLZC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15396
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.052"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            23..1102
                     /gene="mltA"
                     /locus_tag="PXE44_001217"
     CDS             23..1102
                     /gene="mltA"
                     /locus_tag="PXE44_001217"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDL5588732.1"
                     /translation="MAAASVVAIATVLVACSSSGQKGKTTSYDAEKAKFGAKYNGRHF
                     MPYGTHFQAGRLGLNAAPVNQLDFLQQLSNVRAYSSLADSYAIAYGKISRWISSGGNI
                     NDLAKYGISTRLMRGEDGYQNVLMTGYYSPVIKARRTPQGQYQHPIYAMPSSKRFSRA
                     EIYAGALQGQGLELAYSNSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQNGFKYASVG
                     RLLVEDGEIPKEKMSVQAIREWGQRNPSRVQGLLERNPSYVFFKNDPTGQVKGSAGVP
                     LVPLASVASDRSIVPSGSVLLVEMPLIDSKGNWTGKHEMRLMVALDVGGAVKGHHFDL
                     YQGIGDKAGHIAGLMKHYGRVWVLN"
     gene            1115..1564
                     /locus_tag="PXE44_001218"
     CDS             1115..1564
                     /locus_tag="PXE44_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588733.1"
                     /translation="MLKKLSAIFLLLPSAVLANNLQNITASTEPKYKISEIDVRILIR
                     QLNNIEQCIYPELAKPGYQQIYANWNLAENLTMQYFEYQLLKELLGEENQKLMQNDTL
                     STEYFHLLHSRLNHQKANVDPEKCDTFKPRYKEIYKSMENALTKKNQ"
     gene            complement(1598..3115)
                     /gene="purF"
                     /locus_tag="PXE44_001219"
     CDS             complement(1598..3115)
                     /gene="purF"
                     /locus_tag="PXE44_001219"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL5588734.1"
                     /translation="MCGIVGIIGNSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFR
                     LRKANGLVSDVFQQEHMVRLQGNVGIGHVRYPTAGSSSVSEAQPFYVNSPFGITLVHN
                     GNLTNNAELKARLYNEARRHVNTNSDSESLLNIFAYFLDLYSTQHLSPDNIFETVRKT
                     NDSIRGAYACIAMIIGHGMVAFRDPFGIRPLVLGKREIEGKTEYMFASESVALDVVGF
                     EFVRDVLPGEAIYVTFDGQLHSQICADNPKLNPCIFEYVYFARPDSVIDGVSVYSARV
                     HMGELLGEKIKREWGRIIDDIDVVIPIPETSNDIAVRIANMLYKPYRQGFVKNRYVAR
                     TFIMPGQAQRKSSVRRKLNAIASEFKGKSVLLVDDSIVRGTTSEQIVEMARAAGAKRV
                     YFASAAPEIRYPNVYGIDMPTCEELVAYDRSVEEVAQMIGVDKLIFQDLEALYKSIQL
                     ENPTIHRFDDSVFTGEYITGDVDKCYLDSIARSRNDKAKAEAAKQATNLEIHNER"
     gene            complement(3130..3633)
                     /locus_tag="PXE44_001220"
     CDS             complement(3130..3633)
                     /locus_tag="PXE44_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL5588735.1"
                     /translation="MVDLIVIGIIAFSVLISLWRGFTNEALSLLGWVIAFFVASSFYP
                     YLSGYLTQINSVYLQNSEFLRNGIAAAILFILTLIVCGVINGLLGKVIDLSGMSITDR
                     ILGGAFGALRGILIVSAILFFLDTFTQASQTELWKSSLLIPHFDFIVKWFFEQLQANS
                     SFLNTTK"
     gene            complement(3779..4240)
                     /gene="rnhA"
                     /locus_tag="PXE44_001221"
     CDS             complement(3779..4240)
                     /gene="rnhA"
                     /locus_tag="PXE44_001221"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDL5588736.1"
                     /translation="MKLVEIFTDGSCLGNPGKGGIGILLRYNGYEKTVSKGYFQTTNN
                     RMELRAVIEALAMLKEPCKVQLNSDSQYMKNGIQKWIFNWKKNDWKTSDKKPVKNKDL
                     WVALDQEIQRHQIEWSWVKGHSGHRENEICDELAKQGANNPTLDDVGYIAD"
     gene            complement(4333..5664)
                     /gene="argG"
                     /locus_tag="PXE44_001222"
     CDS             complement(4333..5664)
                     /gene="argG"
                     /locus_tag="PXE44_001222"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005722481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDL5588737.1"
                     /translation="MSATILEALPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTA
                     NLGQPDEDDYNAIPKKAMEYGAENARLIDCRQQLAHEGIAAIQCGAFHISTGGIPYFN
                     TTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLD
                     QLFIDELGGRLEMSKFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIV
                     KPIMGVAFWDESVEIKAETVSVTFEEGVPVALNGKRIDDAVELIMEANRIGGRHGLGM
                     SDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQ
                     GRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEK
                     VEDAPFTPADRIGQLTMRNLDIVDTRGKLAVYTDAGLLAANNSVIPQLGKK"
     gene            5848..6888
                     /locus_tag="PXE44_001223"
     CDS             5848..6888
                     /locus_tag="PXE44_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5588738.1"
                     /translation="MKKLLVIRNDKIGDFMVCFPAFAMLKQSMPNVEITALVPSYTAP
                     LAELCPSIDKIIIDTANKKDKNEFNRILQAVKNEQFDAVICFVSDWHNAKLTWQSGIK
                     YRLAPATKLFQFLYNHRLTQRRSQSAKAESEYNLDLARAFLSKHNIPIVEPHTPYLSV
                     EQQTVQAQKAKLCEQLNITTVKKWVFVHSSTGGSANNLSIEQYAELIQGILAEFDCYV
                     VLTAGKGESEKAQQLAQQINHNNVVVYDKNEGLQDFTHSLACADLFIAGSTGPLHISG
                     ALNIPTIGFYPSRLSAIPRRWKPINDANKHIAFCPPSDKASQMNLTLISISEALKTIV
                     PFIRRMWNTPSL"
     gene            complement(6953..7711)
                     /locus_tag="PXE44_001224"
     CDS             complement(6953..7711)
                     /locus_tag="PXE44_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL5588739.1"
                     /translation="MPTLSVAMIVKNEAQDLRACLDTVKDWVDEIVILDSGSTDETPQ
                     IAAEYNARFYTNTDWQGFGKQRQLAQQYVTSDYVLWLDADERVTAELKQSILQAVKND
                     EKNTVYKISRLSEVFGKQIRHSGWYPDCVVRLYRTEFAKYGDELVHEKVHYPKEAKIQ
                     KLQGDLLHFTYKDIHHYLVKSASYAKAWAIQRANAGKTASLFDGVTHAIGCFVKMYLL
                     KAGFLDGKQGFLLAVLSAHSTFVKYADLWNRTRK"
     gene            7838..8152
                     /gene="yhbY"
                     /locus_tag="PXE44_001225"
     CDS             7838..8152
                     /gene="yhbY"
                     /locus_tag="PXE44_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL5588740.1"
                     /translation="MSITLTTKQKQFLKGLAHHLSPVVMLGGNGLTEGVLAEIDNALD
                     HHELIKVKVAGADRETKQLIIDAILRETKAAEVQTIGHILVLYRQSEEKKISLPKATK
                     AI"
     gene            8213..8935
                     /locus_tag="PXE44_001226"
     CDS             8213..8935
                     /locus_tag="PXE44_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL5588741.1"
                     /translation="MFKKFSLYTLAMLLIPVVTWLANWHWSLAIQYAEFDFDYFLYLI
                     TETGSVPYAIITCVVFALLLARVVRKRYSLIFVITVCVASMVATQAIKTAMKSTFQEP
                     RPFVTALFQDKTDDFYQLTREQRGLAVMEKIGANQNFVTAHQAKEVGYSFPSGHTIFA
                     VSWLLLVVGFCRNMRGQAVVLAHFFAISWAALMLISRLRLGMHYPIDLFTSSLIAFLI
                     NLALFVWFVPWLESKKWFAKKP"
     gene            9004..9165
                     /locus_tag="PXE44_001227"
     CDS             9004..9165
                     /locus_tag="PXE44_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588742.1"
                     /translation="MEMSIEKIAELLSDKEKDDYPFSIEERQFAAQASADTWEAILNN
                     TIQPFDFKK"
     gene            9173..9535
                     /locus_tag="PXE44_001228"
     CDS             9173..9535
                     /locus_tag="PXE44_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588743.1"
                     /translation="MYQVFIAEPKDPNLEKGLLKHFIKSYLTQEELDFLHKILTNLPN
                     IRPFWGDTGYKYIVIDNVYFLYEVSESETKVILLGTKFNNKRKTFNFANTKTQQKSMK
                     KWINDLAIYKPHKINQSQ"
     gene            9548..10465
                     /gene="nagK"
                     /locus_tag="PXE44_001229"
     CDS             9548..10465
                     /gene="nagK"
                     /locus_tag="PXE44_001229"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDL5588744.1"
                     /translation="MAIYYGLDVGGTKIELAAFNEKLEKLHSERVPTPQTNYQDWLTA
                     IKTLVETADTKFDEKGTVGLGIPGFVNRETGIAEITNIRVADGKTILKDLAECLGREV
                     RAENDANCFALSEAWDESNKPYSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHM
                     QLNYHALKLLDWDKAPIYECGCGNQACLDTYISGRGFELLFNDLVGEKISAKAIIERF
                     YQQETKVVAFVEKYIELMAISISNLITILDPDMIVFGGGLSNFDYIYQALPKALPKHL
                     MRSAKVPVIKKAIHGDSGGTRGAAALFLK"
     gene            complement(10536..12485)
                     /locus_tag="PXE44_001230"
     CDS             complement(10536..12485)
                     /locus_tag="PXE44_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL5588745.1"
                     /translation="MYQRSQSRTVLFPILLFVGINLVILTLSRLVLGVWQSDRVSAVD
                     GWIPLILQGIRIDISSLCWLFGLPALFSVLCFHKNVLGSIWQTVLRIWLTVGSVFILF
                     MEVATPNFIETFDLRPNRLFIEYLVHPREVFTMLINGHLWAVICGIGIAMISLIIYWK
                     LSGWATKNLHFPSWKMRPLLALLVAAIVFVGGRSSFAHRGINPAMVAFSSDPLVNSLV
                     LNSGYSVLYAVQQLKDEDKSSEQYGKMSVEEMFNIVKQVRNRPASDYISDEIPTLTQN
                     KATYQGKPKNIVIILEESLGAQFVGSLGGKNLTPNLDKLSEQGWYFTNLYATGTRSVR
                     GIEAVTAGFTPTPARSVVKLTKSQTNFFSIAELLKRQGYHTSFIYGGEKHFDNMASFF
                     YGNGFQQIIDEKDYKNPKFTATWGVSDEDLFDKANETFNQLHKSGKPFFSLVFSSSNH
                     DPFEFPDGKIELYEQPKQTRHNAAKYADYAIGHFFELAKKSEYWQDTVFLVIADHDSR
                     AVGEHLVPIQHFHIPALLLGEHIQPRTDNRLVSQLDMPTTLLSVAGISGQYPMIGYDL
                     TQDIDPNRAFMQYDATQAMMRGNDVVVMRPNLPIEGFSYDKANYKLTPKEQPESFKKE
                     ALAHALLGSYLYKNQLYKLPEETKQ"
     gene            complement(12518..13306)
                     /locus_tag="PXE44_001231"
     CDS             complement(12518..13306)
                     /locus_tag="PXE44_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDL5588746.1"
                     /translation="MQVTELNIYPIKSTQAIQVSQAFVEPKGLNFDREFMLAEPNGKF
                     ITARKDPELYRLAAFPIATGVMITHTSGQKCVALYQDFVEEQSSEVWGTHFNAKVAAE
                     AVNQWLSEIFGRAVQLRWLGSQSQRIVANFADHPMSFADSNPVSLMSLKSLEQVQQWS
                     PIPLEMARFRPNIVIDGNVAFEEEQWQQVQIGEVLFTKAALCTRCILITRDLNTLELD
                     PKSEPFRTLKEKHCDEKGKPVFGIHLVPQNSGVIKVGDPVIIQL"
     gene            13431..13661
                     /gene="xseB"
                     /locus_tag="PXE44_001232"
     CDS             13431..13661
                     /gene="xseB"
                     /locus_tag="PXE44_001232"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL5588747.1"
                     /translation="MAKKPTQDFESTLKELEAIVTHLESGDLPLEEALNEFETAIKLV
                     QQGQERLQKAEQRIQILLNKNDTAPLSDYERE"
     gene            13667..14560
                     /gene="ispA"
                     /locus_tag="PXE44_001233"
     CDS             13667..14560
                     /gene="ispA"
                     /locus_tag="PXE44_001233"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDL5588748.1"
                     /translation="MSYLLATDLTHLQQRINHFLAEKLKTTDSVGSPLVEAMAYAVLL
                     GGKRVRPFLIYATGRMLGVPLEKLDHSAAAMEAIHAYSLVHDDLPAMDDDRLRRGKPT
                     CHIAFDEATAILAGDALQSFAFELLATDPHLTDREKVVQVTELATASGAKGMCLGQSL
                     DLIAENKAVSLEELELIHRNKTGALILSSVMMGLNLSEYSQNVAIKQPLVNYAKAIGL
                     AFQVQDDILDVIGETDKIGKIVGSDEHLNKSTYPKLLGLDGAKQKAEMLYQTALHSLE
                     QLPFDTTALKELANFIVKREN"
     gene            complement(14595..15395)
                     /locus_tag="PXE44_001234"
     CDS             complement(14595..15395)
                     /locus_tag="PXE44_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDL5588749.1"
                     /translation="MCQLLGMNCNTPTDITFSFDGFRRRGGLTDHHTDGFGIAFFEGK
                     GVRVFRDNRPGASSPMAEAISQYQIKSLNVIAHIRKATRGDVNLENTHPFIRELWGEN
                     WVFAHNGTVEGVGVCEECHYQPIGTTDSEAAFCRIVSELKEMYPAKPSEKEIFDAVVQ
                     ITSRIAEHGVFNFVLSNGHWMIARCTTNLHYVCRKAPFGKALRDDDVSIDFSQYTKIT
                     DKVTIIATQPLTKNENWTKMKNGGYVFFKDGDVVYEIEGTLPEAQPHV"
BASE COUNT         4692 a         3264 c         2977 g         4463 t
ORIGIN      
        1 ttggaaagca tataaaaaag ctattgcggc agctagcgtg gtagcgattg caacggtatt
       61 agtggcgtgt tcgtcttcag gccaaaaagg caaaacaacc tcttatgatg cggaaaaagc
      121 taaatttgga gcaaaatata atggtcgtca ctttatgcct tatggcacac attttcaagc
      181 tggacgttta ggattaaatg ctgctcctgt taatcaacta gactttttac agcagctttc
      241 taatgtcaga gcctatagta gcttggcaga ttcttatgct atcgcttacg gaaaaatcag
      301 tcgttggatc tcatcgggcg gtaatattaa tgatttagca aaatatggta ttagtactcg
      361 tttaatgcgt ggcgaagatg gctaccagaa tgtgttaatg acagggtatt actcgccggt
      421 tatcaaagcc cgtagaacgc ctcaggggca ataccaacac ccgatttatg cgatgccgtc
      481 tagcaaacgt ttttcacggg cggaaattta cgcaggagca ttgcaaggtc agggcttaga
      541 actggcttat agtaattcaa tgctcgataa cttcttatta ggtgttcaag gtagcggtta
      601 cgttgacttt ggtgatggtc gcttaaacta ttttgcttat gcagggcaaa atggctttaa
      661 atatgccagc gttgggcgtt tattagtaga agatggcgaa attccgaaag agaaaatgtc
      721 agtgcaggct atccgtgaat gggggcaacg taaccctagc cgtgttcaag gtttgttgga
      781 gcgtaatcca tcgtatgttt tctttaaaaa cgatcctacc ggtcaggtaa aaggctctgc
      841 cggtgtgccg ttagtaccac ttgcatctgt tgcatcagat cgtagcattg tgccatcagg
      901 tagcgtgttg ttggtcgaaa tgccgttgat tgatagcaaa ggaaattgga ccggcaaaca
      961 cgaaatgcgt ttaatggtgg ctcttgatgt tggcggtgca gtgaaaggcc atcacttcga
     1021 tttatatcaa gggattggcg ataaagcagg ccatatcgca ggtttaatga aacattacgg
     1081 ccgtgtttgg gtattaaatt aatacagggt aactatgtta aaaaaactat ctgccatatt
     1141 cttacttcta ccgagcgccg tgcttgcaaa taatttgcaa aatataaccg cttcaactga
     1201 gcctaaatat aaaatctcag aaatagatgt cagaattttg atacgccagc tcaataatat
     1261 tgagcaatgt atctatcctg agttagctaa acccggttac cagcaaatct acgcaaattg
     1321 gaatctggct gaaaatttaa cgatgcaata ttttgaatat cagcttctaa aagagttgct
     1381 cggagaggaa aatcagaaac tgatgcaaaa tgatacgctt tctaccgagt atttccattt
     1441 actgcattct cgtttaaatc accaaaaagc aaatgtagat cccgaaaaat gcgatacatt
     1501 taaacctaga tataaagaga tttataaaag tatggaaaat gcactcacta agaaaaatca
     1561 atagtaaaaa actcgcagtt ttcactgcga gtttctttta tctttcgtta tgaatttcta
     1621 aattggtggc ttgttttgcc gcctctgctt ttgctttatc gtttcgagat cttgctatac
     1681 tgtctaaata gcatttatct acatcacctg taatatattc tcctgtaaat acagagtcat
     1741 caaagcgatg aatagtcgga ttttccagtt gaatagactt ataaagtgct tcaaggtctt
     1801 ggaaaatcaa tttatccacc cctatcatct gtgcaacctc ttccactgag cgatcataag
     1861 ccactaattc ttcacaagtc ggcatatcaa tgccatacac attcgggtag cgaatttccg
     1921 gtgcggcaga ggcaaaataa acccgttttg caccagctgc tcgtgccatt tccacgattt
     1981 gttcagacgt tgtacctcgt acaatagaat catcaaccag taacacgctt ttgcctttaa
     2041 attcagaggc aatcgcattt aatttacggc gaaccgagct tttacgctgt gcttgccccg
     2101 gcataataaa agttcgagct acatagcggt ttttaacaaa cccttgacga tagggtttat
     2161 acaacatatt agcaatacgt accgcaatat catttgaggt ttcaggaatc gggatcacca
     2221 catcaatatc atcgataatt cgtccccatt cacgtttaat tttctcacct aataattcgc
     2281 ccatatgcac tcgtgcagaa tatacagaaa cgccatcaat gacggaatca ggacgggcaa
     2341 aataaacata ttcaaaaata caaggattca gttttggatt atcggcacaa atttgcgaat
     2401 gtaattgccc atcaaaggta acataaatcg cttcacccgg cagcacatct cgcacaaatt
     2461 caaaccccac tacatcaaga gccacacttt ccgaagcaaa catatattcg gttttaccct
     2521 cgatttcacg tttacccagc actaacgggc gaataccgaa tgggtcacgg aaagcaacca
     2581 taccgtgtcc gataatcatc gcaatgcaag cataagcacc acgaatgcta tcattggttt
     2641 tacgaaccgt ttcaaagata ttgtctgggc ttaaatgctg agtggaatag agatctaaaa
     2701 agtaagcaaa aatattaaga agggattcag aatcagaatt agtgttcaca tggcggcggg
     2761 cttcgttgta taagcgagct ttaagttccg cattattagt taaattaccg ttgtgaacta
     2821 aggtaatacc gaaaggtgaa ttgacataaa atggctgggc ttcagacaca cttgagctac
     2881 ctgctgttgg gtagcgaacg tgaccaattc caacattgcc ttgtaatctc accatatgct
     2941 cttgctggaa aacatcgctg actaagccgt tagctttgcg taagcggaaa cgattttcat
     3001 cgtctatggt gacgatacct gcggcatctt gtcctcggtg ttgaagtaat gttaaaccat
     3061 cataaatcgc ctgattcacc ggcgaattcc caataatacc gacaatgccg cacatataga
     3121 aaaacctctt tatttagtcg tgtttaaaaa actggaattt gcttgtaact gttcaaagaa
     3181 ccacttcaca ataaagtcaa aatgcggaat taatagcgat gatttccata attccgtttg
     3241 gcttgcttgg gtaaaggtat ctaagaaaaa taaaatagcc gacacaatta aaataccacg
     3301 caatgcccca aaagcaccgc ctaaaatacg gtcggtgata gacattccgc tcaaatcaat
     3361 cactttgcct aataaaccgt tgatcacacc acaaacaatt aaagtcaaaa tgaacagaat
     3421 cgccgctgca ataccattgc gtaaaaattc tgaattttgc aaataaaccg aattaatttg
     3481 tgtgagataa ccgcttaaat aaggataaaa cgagcttgcc acaaaaaagg cgattaccca
     3541 accaagtagc gataatgctt cattggtaaa acctcgccat aaactgataa gcactgaaaa
     3601 agcaatgatt ccgataacaa ttaaatcaac cataacacac cttaaagcgt aaagctctcc
     3661 tattctacaa aaaaagaaaa cgtttgcgta gcactatttc gctaaaaata agtgctttat
     3721 gcaaatacaa gcggttaaat tcggtaaaaa atttacacaa tccgaccgct tgtaaaacct
     3781 agtctgcaat ataaccaaca tcgtccaacg ttggattatt tgctccttgt ttagccagct
     3841 catcgcaaat ctcattttca cgatgtcctg aatgcccttt cacccaactc cattcaattt
     3901 ggtggcgttg aatttcttgg tctaaagcaa cccataaatc tttattttta actggctttt
     3961 tatcgctggt tttccagtca tttttcttcc agttaaaaat ccatttttgg atcccgtttt
     4021 tcatatattg gctgtcacta ttgagttgca ccttacaagg ctcttttagc attgctaaag
     4081 cctcaatcac cgcccgcaat tccattcggt tattggtggt ttgaaaatag cctttgctca
     4141 cggttttttc gtacccgtta taacgcagca aaataccaat cccccctttg ccggggttgc
     4201 ctaaacaaga accgtctgta aaaatttcaa ctaatttcat catcacgctt tgaaaataaa
     4261 agccccaaag cattaacttt ggggcagctt ttaggctaaa ttctaaaagt tatcagaatg
     4321 gagatcaact gattattttt ttccaagttg tggaatcacg ctattattag ccgccaataa
     4381 acctgcatcg gtataaactg ctaatttacc acgggtatcg acaatatcta agttacgcat
     4441 tgttaattga ccgatacgat ctgccggcgt aaacggtgca tcttctactt tttccatact
     4501 taaacgttct gcttcgtagg ttaagtttgg cgattcagtg ttaagaatag tgaaatcgtt
     4561 accacggcgt aactcaagcg tgaccgtacc ggtaatcgct tttgccaccc aacgttgagc
     4621 cgtttcacgc aacattaaag cttgcgggtc aaaccagcgg ccttgataca ataaacgccc
     4681 taaacgtaat ccgttaatgc gatattgctc aatcgtatct tcgttgtgaa taccggtaac
     4741 taaacgctcg taagcgatgt gtaataatgc cattcccggt gcttcataaa taccacgaga
     4801 tttcgcttcg ataatacggt tttcgatttg gtctgacatt cccaaaccat gtctgccacc
     4861 aatgcggtta gcctccataa ttaattcaac ggcatcatca atacgtttac cgtttaacgc
     4921 caccggcaca ccttcttcaa aggtaacaga aacagtttcc gctttaattt caactgattc
     4981 gtcccagaat gccacgccca taatcggttt tacgatcttc ataccggtgc ttaattcttc
     5041 taaatctttc gcttcgtggg ttgcacctaa catattcgag tctgtagagt aggctttttc
     5101 caccgacatt ttatagtcaa aaccgttttc gattaagaat ttagacattt ccaagcgacc
     5161 gcctaattca tcaatgaata gttggtctaa ccacggctta taaattttta attttggatt
     5221 ggttaataaa ccgtaacgat agaaacgctc aatatcgtta cctttaaagg ttgaaccatc
     5281 gccccagata ttcacatcat cttctttcat tgccgcaacc aacattgtgc cggttaccgc
     5341 acgaccaagt ggcgtggtgt tgaaatacgg aataccgccg gttgaaatat ggaaagcacc
     5401 gcattgaatt gcggcaatgc cttcgtgagc taactgttgg cgacagtcga ttaatcgtgc
     5461 attttcagcc ccatattcca tcgctttttt cggaattgcg ttgtaatcat cttcatccgg
     5521 ctgacctaaa tttgcggtgt acgcataagg cactgcccct tttttacgca tccataacag
     5581 tgctgctgag gtatctaaac cgcctgaaaa ggcgataccc actttctgcc ctaaaggtaa
     5641 agcttctaaa attgttgctg acatacgaat tcctataaat tttgaaagta aaaatattca
     5701 aacgcaaacg cttacgttca agcaaaatat tctagctgaa aagtagtaaa aattcatcta
     5761 aaatttagcc cgatgatttg caaatttttt gtgaaatttg accgcttata atttactgaa
     5821 aacctaaaag caatagagaa aatcagaatg aaaaaactac tcgtaatccg taatgataaa
     5881 attggggact ttatggtctg ctttccggca tttgctatgt tgaagcaatc aatgccgaat
     5941 gtggaaatta ccgctcttgt gccaagttat actgctccac ttgccgagct ttgcccttct
     6001 attgataaga ttattattga tacggcgaat aaaaaagata aaaatgaatt taaccgtatt
     6061 ttacaagcgg ttaaaaatga gcaatttgat gcggttattt gttttgtttc agactggcat
     6121 aatgccaaac tcacctggca aagtgggatt aaataccgcc tcgctccagc aactaaatta
     6181 ttccaatttt tatataacca ccgtttaacc caacgccgtt ctcaatcagc aaaagcggaa
     6241 tccgaatata atttggattt agcccgtgct tttttaagca aacacaatat accgattgtt
     6301 gaacctcaca cgccatactt aagtgttgaa cagcaaaccg tccaggcaca aaaagcaaaa
     6361 ttatgcgaac aactcaatat taccaccgtt aaaaagtggg tatttgtgca tagttcaacc
     6421 ggtggttcgg caaataattt atctatagaa cagtatgcag agttaattca aggtattttg
     6481 gcagaatttg attgctatgt ggtactcacc gcagggaaag gcgaaagcga aaaagctcag
     6541 cagttagctc aacaaatcaa tcacaacaac gtggtggttt atgataaaaa tgaggggtta
     6601 caagatttta cacattcgct tgcgtgtgca gatttgttta ttgccggctc aaccggtccg
     6661 ttacatatta gtggggcgtt aaatattcca actattggct tctacccgag ccgactttct
     6721 gccattccac gccgttggaa accgattaat gatgcgaata aacacattgc attttgccca
     6781 ccgtctgata aagcctcaca aatgaattta actttaattt ctatttctga ggcattaaaa
     6841 acgatagtac cgtttattcg gagaatgtgg aatactccct ctctctgata aaattgctaa
     6901 acgcaatttt atctatctct ctcccactag agggagagag tactgaaaag catcatttcc
     6961 gagtacgatt ccacaaatcc gcatatttta cgaaagtaga atgtgccgaa agaactgcca
     7021 ataaaaatcc ttgcttgcca tctaaaaaac cggcttttaa tagatacatt ttcacaaaac
     7081 agccgatggc gtgagtcacg ccatcaaata agctggctgt tttgcctgca tttgcccgtt
     7141 ggattgccca cgctttagcg taacttgccg attttaccaa atagtggtga atatctttat
     7201 aggtgaaatg cagcaaatcg ccttgtagtt tctggatttt tgcctctttt gggtaatgta
     7261 ctttttcgtg aaccaattca tcaccatatt tggcaaattc cgtgcgatac aatctcacca
     7321 cacaatccgg ataccaaccc gagtggcgaa tttgtttgcc aaatacttca cttaagcggc
     7381 tgattttgta aacggtattt ttttcatcat ttttgaccgc ttgtaaaata gattgcttca
     7441 gttcggctgt cactcgctca tctgcatcaa gccaaagtac ataatcactg gtcacatatt
     7501 gttgagctaa ctgacgctgt ttaccaaagc cttgccaatc ggtgttagtg tagaaccttg
     7561 cgttgtattc agcagcaatt tgtggtgttt catcagtact gccactatct aaaatcacaa
     7621 tttcatctac ccaatctttt actgtgtcaa ggcaagcccg taaatcttga gcttcatttt
     7681 tgacaatcat cgcaacactt aatgtcggca tagtttatcc ttctctcttt ttgtcgtgtg
     7741 tttttctgct attcgttgca ttttacttta aaaatagtgg tttttgtgct attttctacg
     7801 ccaaatttta tcaacaaaac aaattaacgg aaaaagaatg tcaattacat taacaaccaa
     7861 acaaaaacaa ttcctaaaag gcttggctca tcatttaagc ccagtggtga tgctgggggg
     7921 taacggctta acagaaggcg tgttagctga aatcgataac gccttagacc accacgaact
     7981 gattaaagtt aaagttgccg gggctgatcg tgaaaccaaa caattaatta ttgatgccat
     8041 tctgcgtgaa actaaagcgg ctgaagtaca aaccattggg cacattttag tgctttaccg
     8101 ccaaagcgaa gagaaaaaaa tcagcttgcc aaaagcaact aaagcgatct aattgcaaaa
     8161 atatgtttaa attcaaccgc ttgtattcaa gcggtttttt ataggacttt ctatgtttaa
     8221 aaaattctct ctttataccc ttgcaatgtt gctcattcct gtggtgactt ggctggcaaa
     8281 ttggcactgg tctctggcaa ttcaatacgc tgaatttgat ttcgactact ttttatattt
     8341 aatcaccgaa accggctccg taccttatgc catcatcacc tgtgtggttt tcgccttatt
     8401 actggcgagg gtggttcgca aacgctactc tttaattttt gtgattaccg tgtgtgtcgc
     8461 ttcaatggta gccactcaag caatcaaaac agcgatgaaa tcaacctttc aagagcctcg
     8521 tccatttgtc actgctcttt tccaagataa aacagacgat ttttatcaat taacacgcga
     8581 gcaacgtggg cttgccgtaa tggaaaaaat cggtgcaaat caaaattttg ttaccgctca
     8641 ccaagccaaa gaagtgggct attctttccc atcaggccac actatttttg cggtttcttg
     8701 gctattattg gtggtaggtt tctgccgtaa tatgcgagga caagcggtcg ttttggctca
     8761 tttttttgca atcagttggg cggcgttaat gctgattagc cgcttgcgtt taggtatgca
     8821 ctacccgatt gatttattca ccagtagttt aattgcgttt ctaatcaatt tagccttatt
     8881 tgtgtggttt gtgccttggt tggaaagtaa aaaatggttt gctaaaaagc cttaattggc
     8941 aatttagcca aatcaatata caatacaaat tattgaataa gtacctaaaa taaggagctt
     9001 agaatggaaa tgtcgataga aaaaatagct gaactattaa gcgataaaga aaaagatgac
     9061 tatccgttta gtattgagga aagacaattt gccgcccagg ctagtgctga tacttgggag
     9121 gcaatcttaa ataacactat tcaacctttt gacttcaaaa aataggcttg ctatgtatca
     9181 agtttttatt gcagaaccaa aagacccaaa cttagagaaa ggcttattga aacacttcat
     9241 taagtcttat cttactcaag aagaactcga tttcctgcat aaaattctca ctaatttacc
     9301 caacatcaga cctttttggg gcgataccgg ttacaaatat atcgtcattg ataatgttta
     9361 cttcttgtat gaagtttccg aaagtgaaac gaaagtcatt ctacttggca ccaaatttaa
     9421 taataaacgg aaaactttta attttgcgaa tacgaaaacc cagcaaaaat caatgaagaa
     9481 atggattaat gatttagcaa tttataaacc gcataaaatc aatcaatcgc aataagagag
     9541 cgtaattatg gcaatttatt atggcttaga tgtgggcggc acaaaaattg aattagccgc
     9601 ttttaatgaa aaactagaaa aactacacag cgaacgtgtg ccgacaccgc agacaaatta
     9661 tcaagattgg ctgacggcaa ttaaaacgct ggttgagact gccgatacaa aatttgacga
     9721 aaaaggcact gtcgggctgg gcattccggg ctttgtaaac cgtgaaaccg gcattgcgga
     9781 aattaccaat attcgagtgg ctgacggcaa aacgattctg aaagatttag ccgaatgttt
     9841 agggcgtgaa gtgcgtgccg aaaacgatgc taactgcttt gccctgtctg aagcgtggga
     9901 cgaaagtaat aagccttatt ccaccgtatt agggttgatt atcggcaccg gttttggcgg
     9961 tggttttgtg tttgacggta aaattcattc aggcagaatc ggaatggcag gcgaagttgg
    10021 gcatatgcaa cttaactacc acgcacttaa attgctcgac tgggataaag ccccaattta
    10081 tgaatgtggt tgtggcaacc aagcctgttt agacacctat atttcagggc ggggttttga
    10141 gttactgttt aatgatttag taggagaaaa aatctcggcg aaagcgatta tcgaacgctt
    10201 ctaccaacaa gaaaccaaag tggtggcatt tgtcgaaaaa tatattgagc taatggcaat
    10261 cagtatcagc aatctgatta cgattttaga tccggatatg attgtattcg gtggcggttt
    10321 atccaacttt gattatatct accaagctct accaaaagca ttaccaaaac acttaatgcg
    10381 ttcagcgaaa gtgccggtca tcaaaaaagc aattcacggc gactcaggtg gcactcgtgg
    10441 tgcagcagca ttattcttaa agtaatgaac aaatttgaga gagggcaagc ggtcattttt
    10501 aataaaaata ttgcagccct ctcttttaat ttgccttact gcttagtttc ttccggtaat
    10561 ttatataact gatttttata taaataactg ccaagcaatg cgtgtgctaa cgcctctttt
    10621 ttaaacgatt ccggctgttc tttaggggtg agtttatagt tagctttatc gtagctaaat
    10681 ccttctatcg gtaaatttgg tcgcattacc accacatcat ttccccgcat catcgcctgt
    10741 gtcgcatcat attgcataaa ggcacggttt gggtcaatgt cttgcgttaa atcataccct
    10801 atcatcggat attgaccact tattcccgcc acagaaagta atgttgtcgg catatcgagc
    10861 tgactcacta aacggttatc cgttcttggc tgaatgtgtt cgcctaataa aagtgccgga
    10921 atatggaagt gttgaattgg cactaaatgc tcgccaacgg ctcttgaatc gtgatctgca
    10981 atcactaaaa acaccgtatc ttgccagtac tctgattttt tcgctaactc aaagaaatgc
    11041 ccgatagcat aatcggcata ttttgccgca ttatggcgag tctgcttcgg ttgttcatag
    11101 agttcaattt tgccgtccgg aaattcaaac ggatcgtggt tactggaact gaataccaaa
    11161 ctaaaaaacg gtttacctga tttgtgcagt tgattaaaag tttcatttgc cttatcaaac
    11221 agatcttcat cactcacgcc ccaagttgcg gtaaatttag ggtttttata atctttttca
    11281 tcgataattt gctggaaacc attaccgtag aaaaagctcg ccatattatc aaaatgcttc
    11341 tccccaccat aaataaagga agtatggtat ccttgacgtt ttaataattc ggcaatacta
    11401 aaaaagttag tttggctttt cgtcagtttt accacagatc ttgccggtgt tggtgtaaaa
    11461 ccagccgtta ccgcttcaat tcctctcacc gaacgagtac ctgttgcata aagattggta
    11521 aaataccagc cttgttctga aagtttatct aaatttggag tgagattttt ccctcctagc
    11581 gaaccgacaa attgagcccc taaactttct tctaagataa tcacaatgtt tttaggcttt
    11641 ccttgataag tcgctttatt ttgcgtgagg gtgggaattt catctgaaat ataatctgaa
    11701 gctggtcgat tacgaacctg cttaacaatg ttgaacattt cctccacaga catttttcca
    11761 tactgttcgg aagatttatc ttcatctttc aattgctgaa ccgcataaag cacggaataa
    11821 cctgaattta gcaccagtga attgacaagc ggatcgcttg aaaatgccac cattgccgga
    11881 ttaatccctc gatgggcaaa actggaacgc ccaccaacaa aaactattgc agcaaccaat
    11941 aaagccagta gcggacgcat tttccagctc gggaaatgca aatttttggt tgcccaaccc
    12001 gataatttcc aataaatgat gagagaaatc atcgcaatgc caatcccaca aataacagcc
    12061 cataaatgtc cgttaatcag cattgtgaac acttctctcg gatggaccaa atactcaatg
    12121 aaaagacgat ttgggcgaag gtcaaaggtt tcaataaaat tcggtgtggc gacttccata
    12181 aaaagaataa atacactacc gacagttagc caaattctta aaacagtctg ccaaatcgaa
    12241 cccagtacat ttttatgaaa acacaacacg ctaaataatg ccggtaagcc aaatagccaa
    12301 catagcgaag atatatcaat gcgaatacct tgtagtatta atggtatcca tccatcaaca
    12361 gcggatactc gatcagattg ccaaacgcct aacactaaac gggataaggt gaggataact
    12421 aaattaatcc caacaaatag taggattgga aatagcacag ttcttgattg actgcgttgg
    12481 tacattttga atctcctttt aaaaaaatat cggcatatta taattgaata atgacaggat
    12541 cgcctacttt tatcacacca ctgttttgtg gcacaaggtg aataccgaac accggcttac
    12601 ctttttcatc gcaatgcttt tcttttaagg ttctaaacgg ctccgatttg gggtctaact
    12661 caagcgtgtt taaatcccgt gtaatcaaaa tgcaacgagt acataatgcc gctttggtaa
    12721 atagcacttc gccaatttga acttgttgcc actgctcttc ttcaaatgcc acattgccat
    12781 caatcacaat attcgggcga aaacgagcca tttcgagtgg aatcggcgac cattgttgca
    12841 cttgctcaag cgattttaga gacatcaaac tcaccgggtt gctatctgca aaactcattg
    12901 ggtgatcggc aaaattcgca acaattcgtt ggctttgcga gccaagccaa cgtagttgca
    12961 cagccctacc aaaaatttca ctcagccatt gattgaccgc ttctgccgcc actttggcat
    13021 taaaatgtgt tccccacact tcactactct gctcttccac aaaatcttgg taaagtgcaa
    13081 cgcatttttg accgcttgta tgggtaatca ttacccctgt cgcaatcggg aaagccgcta
    13141 gacggtataa ttccggatct ttacgggcag taataaattt gccgttaggc tcggctagca
    13201 taaattcacg gtcaaaattt aagcctttcg gttcaacaaa tgcctgtgaa acttgaatag
    13261 cttgagttga ttttatcggg taaatattga gctcggtaac ttgcatagaa aatcctccat
    13321 ttggaaatag gataacaata cctgcaagcg gtcgaaaagt aaaaggaatt tgcaaatccc
    13381 ttgtgagaac ggtaaacttg agcaactttt acagcaatag gtcagcaaca atggcaaaga
    13441 aacccactca agattttgaa agcacactta aagaattaga agcaatcgtt acccatttgg
    13501 aatccggtga tttaccgctt gaagaagccc tcaacgaatt tgaaactgca atcaaattag
    13561 tgcaacaagg gcaagaacgg ttacaaaaag ccgagcaacg tattcaaatc ttattaaata
    13621 aaaacgatac tgccccactt agcgattacg aaagagaata atcacaatga gctatctatt
    13681 agcaaccgat ctgactcacc tgcaacaacg gattaaccat tttctcgctg aaaaattaaa
    13741 aacaacggat agcgttggtt cgcctttagt agaagcaatg gcttatgcgg tattgctagg
    13801 cggtaaacgg gtgcgaccgt ttttaatcta tgccaccggc agaatgcttg gtgtgccgtt
    13861 ggaaaaatta gatcatagtg cggcggcaat ggaggctatt cacgcttact cgctggttca
    13921 cgatgattta ccggcaatgg acgatgatag attacgccgt ggcaagccaa cctgccatat
    13981 tgcctttgat gaagctaccg ctattttagc cggtgatgcg ttacaaagtt ttgcgtttga
    14041 actgctggca accgatccgc atttgaccga ccgagaaaaa gttgttcaag ttacagaatt
    14101 ggcaaccgct tccggtgcaa aaggaatgtg cttagggcaa agtttggatt tgattgcgga
    14161 aaataaagcg gtgagcttgg aagagcttga attaattcat cgcaacaaaa ccggggcatt
    14221 gattctatca tccgtgatga tgggattaaa cctttccgaa tattcgcaaa atgtagccat
    14281 aaaacaaccg cttgtaaact atgccaaagc gattggctta gcctttcagg tgcaagatga
    14341 tattttagat gtcattggcg aaacagataa aatcggcaaa atagtgggtt cagatgaaca
    14401 cttaaataaa agcacatacc cgaaattgtt gggcttagac ggggcaaaac aaaaagccga
    14461 aatgctttat caaaccgctc tccattcgct ggaacagttg cctttcgata ccaccgcatt
    14521 aaaagagtta gctaatttta ttgttaagcg tgaaaactaa gaaaaaacct agccgaagct
    14581 aggtttttta gtgattaaac gtgaggctga gcttccggca atgtgccttc aatttcatat
    14641 accacatcgc cgtctttaaa gaaaacatag ccaccgtttt tcattttggt ccagttttca
    14701 tttttggtaa gtggctgagt ggcaataatc gtcactttat cggtaatttt ggtatattga
    14761 ctaaaatcaa tcgacacatc atcatcacgc aaggctttgc caaacggtgc tttacggcaa
    14821 acgtaatgca gattagttgt acaacgggca atcatccaat gaccgttgga taaaacaaaa
    14881 ttaaataccc cgtgttccgc aattcttgag gtgatttgca ccacggcatc aaaaatctct
    14941 ttttcgcttg gtttagccgg atacatctct tttaattccg acacaatacg gcaaaatgcc
    15001 gcttcggaat cggttgtacc aatcggctgg tagtggcact cctcgcatac cccaacacct
    15061 tccactgtgc cgttatgggc aaacacccag ttctcgcccc ataattcacg aatgaagggg
    15121 tgggtatttt ccaaattcac atcaccacgc gtagctttac gaatatgtgc aataacattg
    15181 agcgatttaa tttgatattg gctgattgct tctgccatcg gcgatgatgc tcccgggcgg
    15241 ttatcacgaa aaacccgaac ccctttgcct tcaaaaaagg caattccaaa cccatcagta
    15301 tggtgatccg tcagcccacc ccgacggcga aatccatcaa aagaaaaagt aatatcggtt
    15361 ggcgtattac aattcattcc aagtaattga cacata
//
LOCUS       DANLZC010000053        15211 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.053, whole genome shotgun sequence.
ACCESSION   DANLZC010000053 DANLZC010000000
VERSION     DANLZC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15211
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.053"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            29..877
                     /locus_tag="PXE44_001235"
     CDS             29..877
                     /locus_tag="PXE44_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL5588750.1"
                     /translation="MEKKALFRGFLLSTVALAVAACGNLSKVSDEGTTENPVFPKISE
                     SEFNHDGSQFGSWPNWENVRQIEKGMNKDQLYNLIGRPHFEEGLYGVREWDYAFNYRE
                     NGVHKICQFKILFDKNMNAQSFFWYPNGCNGNSSFALTGDFLFDFDKATLTTKGKEVV
                     DNVAQQLKASKAQQVKVAGYTDRLGSAAYNLDLSQRRSNTVKARLVQQGVTAQIEAVG
                     YGKANQVKACDGETGQALKDCLRPNRRVEISANCGVMKQNEGGNVAGPNGPAPLYQTP
                     AYDGSK"
     gene            965..1783
                     /locus_tag="PXE44_001236"
     CDS             965..1783
                     /locus_tag="PXE44_001236"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HDL5588751.1"
                     /translation="MNYQAIAFDLDGTLLSSNATILESSKEAIKKVREKGIKVYFVTG
                     RHHTAVRPYYVELGLDTPVVCCNGTYVYDFKNEKVLSGNPLSAEIASKLVNDAQAQGI
                     HTAVYFEDAMTYESLNIHFTKFQKWVESCAPEVRPNVYQVENFQQEIDKGVTVWKVLI
                     SDPDLAKMQKFVEKLPPDQVSPEWSWVDRVDITSVGNSKGARLAEVLKTEGIDLQKVI
                     AFGDNFNDVSMLETVGLGIAMGNSEPDIQQKAKKTIGSNNEDSIANELKAQFNL"
     gene            1786..3477
                     /gene="narQ"
                     /locus_tag="PXE44_001237"
     CDS             1786..3477
                     /gene="narQ"
                     /locus_tag="PXE44_001237"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDL5588752.1"
                     /translation="MIKAKYSIARRIGLYFLIMIGFASIISGISLGIMWSNKSDASLI
                     NVSGSLRMQSYRLLSEMDSKKALLPERLLEYHRTLHATELSSLKQSVLLPDSVIESYQ
                     QLLKDWYEMQSYVAQGDKVNYASHIEDYVQRVDDFVFRLQEFAELKLKIATGVIAIAM
                     LLIIALAYIGVWYTRKKIIGPLRQLVKASWQIQNKDFDHVKLAVNEPNELGLLSSTFT
                     EMSNELLKLYTSLEDKVQDKTRRLLAVNRSLLVLYQCSQLLTAKPINKEVLNQVLKTV
                     FDNEHLLGIDLQVYGAEYWNVSIDNKPSLDWVSAEIAIENEKLGILSWKPSLVCPGER
                     LIQNVSEMIGRSLYVVQMQKQQQQLVLMEERAIIARELHDSLAQSLTFFKIQVSLLKL
                     AKTDEKRNEILTVFEKALNDAYSQLRELLTTFRLTIQEANLQQALEKILESLRPKTSA
                     QIRLSCKLPSHMFNAQQQVHVLQIVREAVINAIKHSQATEINVIAETNDDGEHCLTIQ
                     DNGVGLAAHTQPEGHYGLTIMQERAAELKAEFSIENVESGGVKVQVLLPSMISKR"
     gene            3528..4046
                     /locus_tag="PXE44_001238"
     CDS             3528..4046
                     /locus_tag="PXE44_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL5588753.1"
                     /translation="MQFRKAVEADFETILAIYNQAIPTHQITADLELATTQNRRKWFD
                     FHLETAQYPIWVVEDEKGIAGWFSFSPFYERPAFLHTSEISIYLDRYSKGKGYGSKII
                     EFMQAEMLNHNIHTLMAYVFELNTVSQNLMRKHGFEQWGRFPNIANMGKDEKGEDKWR
                     TLLMMSYQKGIE"
     gene            4089..4688
                     /gene="rdgB"
                     /locus_tag="PXE44_001239"
     CDS             4089..4688
                     /gene="rdgB"
                     /locus_tag="PXE44_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HDL5588754.1"
                     /translation="MEKTKIVLATSNADKVKEMADVLSQFGFEVVAQSEFGIESPEET
                     GLTFVENALIKARFAAKMTGLPAIADDSGLSVMALGGEPGLYSARYAGEQATDADNRQ
                     KLLAKMQGQENRLAKFVSCIVFLKHETDPTPYIASGECFGEILTEERGENGFGYDSLF
                     FYPPKACTFAELETKEKKAISHRAIALEQLKQQLAGENK"
     gene            4685..5005
                     /locus_tag="PXE44_001240"
     CDS             4685..5005
                     /locus_tag="PXE44_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HDL5588755.1"
                     /translation="MIITTTPTIEGKQIVEYKGLVFGEVVSGANFIRDFLAGITDIIG
                     GRSGAYESKLNGARKEALAELAKEAQKVGANAVVGVSMEYQSMGGDKGMFIVVATGTA
                     VVIR"
     gene            5009..5461
                     /locus_tag="PXE44_001241"
     CDS             5009..5461
                     /locus_tag="PXE44_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDL5588756.1"
                     /translation="MQFEQHFEANFWQNKSLLEMNDAEWEALCDGCGKCCYRKYIQGR
                     GRRERLYYTSVACNLLDVNTGRCMNYSNRFKIESDCTKLTKKNLPDFGWLPKTCAYRL
                     LYEGKPLFDWHPLISKDPDSVKNAGMLIANGIHEKDVIDWFEFVIDEV"
     gene            complement(5502..6083)
                     /gene="lpcA"
                     /locus_tag="PXE44_001242"
     CDS             complement(5502..6083)
                     /gene="lpcA"
                     /locus_tag="PXE44_001242"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDL5588757.1"
                     /translation="MYLEQIKAELQEAADVLDKFMSDEKNIKLIQDAAMLIANSFKQG
                     GKVISCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHLSCVSNDFGYEYVFS
                     RYLEAVGQKGDVLFGLSTSGNSKNVLNAIKVAKEKGMKVIAMTGKDGGQMAGLADVEI
                     RVPHFRYADRIQEIHIKVIHILMMLIEFEMEKA"
     gene            6392..7351
                     /locus_tag="PXE44_001243"
     CDS             6392..7351
                     /locus_tag="PXE44_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL5588758.1"
                     /translation="MVVNPESFEQIIGQSVKIKEVVEQAKKFANLDAPLLIQGETGTG
                     KDLFAKSCHHFGSRRMQKFIAVNCAGLPAEEAESEMFGHRGNGVESVGFFEYAHGGTV
                     LLDSISELSLEMQAKLLRFLNDGSFRRVGEDQEIQVNVRVICTSLQPLSLLVEKGKVR
                     EDLFHRLNVLTLNLPPLRDRKEDIPLLADYFIAQISNQLGVSKLEYDNVFLQALQNYH
                     WRGNLRELYNALYRACTLSHSYRLAVKDLHLPRDMEAMIEVREEATLEEMMSQYEAQL
                     LHQFYAEYPSTRKLAQRLGLSHTAVANKLRQYGLTKNGNSKDE"
     gene            7344..9095
                     /locus_tag="PXE44_001244"
     CDS             7344..9095
                     /locus_tag="PXE44_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5588759.1"
                     /translation="MNKLAVIFSVCFANLGLNSTAYAAPSIPQELLENSLVYCTTSSG
                     FSFNPQKSDVSSNMNVVTEQIYDKLVEFNPKKNRLEPALAESYSISDDGLVITLRLRK
                     NVAFHTTPWFTPSRKLNAEDVVFSLKRMIGKESDLPEVKSEPESVLHHQRQSYIYQKT
                     AGQIYFPYFESVDLANKIASIHSPSNDIVKIELVRPDSALLAHLASQYAVILSKEYAL
                     QLNADDNLVQLDLLPVGTGVYQLESYENNDYVRLKPNMNYWGKKAYIQNMVVDFSTEA
                     TGRMAKFLNNECDVVAFPEPSQLSVLNNRQLISSKGANLVYLAFNMQRGIGQDLALRQ
                     RIARGINRKRIAEKLFYGTGEVADNLLPTALWSKKNNGSYPYQPHQNSGEQAVEMHDS
                     IINRSLDSKTETEEVNQKQRLKLWVIDEKRVYNQHPLKMAEMIRADLATQNLEVEIRA
                     VSRAYLVQQLANKSADYDLILGGWLANNLDPNSFLEALLSCKAAETVTNLSNWCNEDF
                     DFWLQTARLSDDPASANLLYEFAQKLLEEQLPILPLMNANRVLVIKDGVKQADISPFG
                     QVKLSELRLGLGHKKGN"
     gene            9098..10063
                     /locus_tag="PXE44_001245"
     CDS             9098..10063
                     /locus_tag="PXE44_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5588760.1"
                     /translation="MLIVFFRKIFLTLITLIFLSLISFNILLRDPLNHLAELGWFEAY
                     CYYVKSLLNGDFGISFSNGEPLTAQILQVFPATITLCLSALIVSLIIGLPLGFFAATQ
                     QKNMVGRLLNTLGSLSLAVPVFWLALVLMYYASLNQWEVSAIGEIHSLYPKQMVTGFL
                     LLDIWLSDVPYKLKMMQSALHHLALPTLVLAVPATLEVMHITQARAGYVMKQNYIKIA
                     KTRGWTPFRVWRTHIICNTLPALLPMIARTFILIFAFGMLIENIFSWGGIGRWLINAL
                     AIQDYNAISAGVVAIGVFVLLVDMVTGFVRVMLDPSDKKDWYGSY"
     gene            10050..10943
                     /locus_tag="PXE44_001246"
     CDS             10050..10943
                     /locus_tag="PXE44_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5588761.1"
                     /translation="MAVIKEPEQFRETNAFKAFWFHFRQDKLALGSFYLFLLLLALIF
                     VGSLLAPYNYNEQFVGLELMPPSWDDKGQISHFLGTDDLGRDILSRIMYGFYYTLGSA
                     LMITLLIAIIGGMIGIYAGLKKGRSFWLISHLFDTFLFMPILLIAIIIATLMEASLVN
                     AMLAIFLAMLPHFIHKIYQATEKQLKKEYIITLKLDGASCWYLIREVIIPNLTGVAIK
                     ALSHIFVIAILDISALSFIMLGAKSPTPEWGAMIRESMELIYIAPWTVILPGLAIIFS
                     IFTVTILGTRLSVIFEKYRKE"
     gene            11014..12066
                     /locus_tag="PXE44_001247"
     CDS             11014..12066
                     /locus_tag="PXE44_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5588762.1"
                     /translation="MALLDIRHLSIEIDMPKGRVKMVDNINLTVDEGEICGLVGESGS
                     GKSLIAKVICGMIKDEWIVSADRFRFDDIELLKLSPAERRKLVGDHISMVFQDPLTSL
                     DPSQQIGKQLMQTIQFRGKWWQRFGWKKKKAIELLHRVGIRDHQDIMKSYPSDITEGE
                     AQKVMIAMAVANQPRLLVADEPTNIMESTTQLQVYRLLSSMNQNLGTSILLVSNDMRS
                     IQNWVDSYNVLYCGQTVEIADKESILESSYHPYTRVLLNSSPDFSQPLAFKSSLNTLR
                     GSVPMLQEMPIGCRLGPRCPFAQKKCINKPPLVKVKQHEFACHFPINFREQNLLKNKT
                     ENTPFVVAEIAEKEAF"
     gene            complement(12075..12305)
                     /locus_tag="PXE44_001248"
     CDS             complement(12075..12305)
                     /locus_tag="PXE44_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDL5588763.1"
                     /translation="MKLTACKLVFLLYLMFNLTACGTVLSLVEQDYRVYAGVIKDFQV
                     MQEGGIFAVLAVIDLPLSFVLDTLMLPVTLSQ"
     gene            12435..13517
                     /gene="leuB"
                     /locus_tag="PXE44_001249"
     CDS             12435..13517
                     /gene="leuB"
                     /locus_tag="PXE44_001249"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDL5588764.1"
                     /translation="MQTYNIAVLAGDGIGPEVMAEAIKVLEATQQKFGFKLNFNQYNI
                     GGAAIDAQGKALPENTLKGCEEADAILFGSVGGPKWTHLLPDEQPERGSLLPLRKHFS
                     LFCNLRPAALYQGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGRDGEGAQTRA
                     FDTEVYYKYEIERITRVAFESAMKRKKHVTSVDKANVLQSSILWRETVTEVAKEFPEV
                     TLEHMYIDNATMQLIKAPESFDILLCSNIFGDIISDEAAMITGSMGMLPSASLNEQGF
                     GLYEPAGGSAPDIAGKNIANPIAQILSAAMMLRYSFNLGDAADAIETAIQKALADGYR
                     TADLADDSKPLSTSEMGDIIAKNILA"
     gene            complement(13604..13999)
                     /gene="msrB"
                     /locus_tag="PXE44_001250"
     CDS             complement(13604..13999)
                     /gene="msrB"
                     /locus_tag="PXE44_001250"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL5588765.1"
                     /translation="MIKPIHALTEQQVDILINHGTERPFTGKFLNEHRVGIYRCARCH
                     NDLFHSDTKFDAGCGWPSFYQTISESSLRYLDDYSLGRHRIEIRCGNCDSHMGHVFPD
                     GPPPTGLRFCLNSAALNFKWEETGEEIDG"
     gene            14224..>15211
                     /gene="gap"
                     /locus_tag="PXE44_001251"
     CDS             14224..>15211
                     /gene="gap"
                     /locus_tag="PXE44_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL5588766.1"
                     /translation="MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVDYMAYM
                     LKYDSTHGRFDGTVEVKDGQLVVNGKAIRVTAERDPANLKWNEIGVDIAVEATGLFLD
                     DETARKHITAGAKKVVLTGPSKDATPMFVNGVNFDTYAGQDIVSNASCTTNCLAPLAK
                     VIHEKFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPALNGKLTGMAFRVPTTNVSVVDLTVNLEKPATYAEICAEIKRASENEMKGVLGYTE
                     DAVVSTDFNGATETSVFDAAAGIALTDTFVKLVSWYDNEVGYSNKVLDLVAHV"
BASE COUNT         4781 a         2607 c         3254 g         4569 t
ORIGIN      
        1 gattactata aggaaactta atatgaaaat ggaaaaaaaa gcccttttcc gtggattttt
       61 attatcgaca gtggcattag cagtggctgc ttgtggtaac ttaagtaaag tgagtgatga
      121 aggaacaact gaaaacccgg tattcccgaa aatctcagaa tctgaattta accacgatgg
      181 ttcacaattt ggttcatggc caaactggga aaacgttcgt caaattgaaa aagggatgaa
      241 caaagatcaa ttatataatc taattggtcg tcctcatttt gaagaaggct tatatggtgt
      301 tcgtgaatgg gactatgcat tcaattaccg tgaaaatggc gtacataaaa tttgccaatt
      361 taaaatttta ttcgataaaa atatgaatgc acaaagtttc ttctggtatc caaatggatg
      421 taatggaaac tcatcatttg ctttaactgg cgatttctta ttcgatttcg ataaagcgac
      481 tttaactaca aaaggtaaag aagttgttga taatgttgct caacaattaa aagcatctaa
      541 agcacaacaa gttaaagtag caggctatac agaccgttta ggctctgctg cttataactt
      601 agacttatca cagcgtcgtt ctaatacagt taaagctcgt ttagtacaac aaggcgttac
      661 agcacaaatt gaagcggtag gttatggtaa agcgaatcaa gtaaaagctt gtgatggtga
      721 aactggtcaa gcattaaaag attgtttacg ccctaaccgt cgtgttgaaa tttctgcaaa
      781 ttgtggtgta atgaaacaaa atgaaggtgg caatgttgca ggtcctaatg gtccggctcc
      841 actttatcaa accccagcat atgatggcag taaataattt ctagttttga ctaacttaga
      901 gtagaatatt aaggcaacca aatggttgcc ttttctttta tctatttatt tataggggaa
      961 tactatgaac taccaagcta ttgcatttga tttagacgga acactacttt catcgaacgc
     1021 aacaatttta gaatccagta aagaggctat taaaaaggta agagaaaagg gaattaaagt
     1081 ctactttgtt accggtaggc accatactgc ggttcgccct tattatgtag aactagggct
     1141 ggatacgcca gttgtgtgtt gcaatggcac ttatgtgtat gattttaaaa atgaaaaagt
     1201 attaagcggt aatccgttat cagcagaaat tgcttcaaaa ttagttaatg atgcacaagc
     1261 tcaagggatt catactgcgg tttattttga agatgcaatg acttatgagt ctttgaatat
     1321 tcacttcact aaattccaaa aatgggttga gtcttgcgca ccagaagttc gtccgaatgt
     1381 ttatcaggta gaaaatttcc aacaagaaat tgataaaggt gtaaccgtat ggaaagtgct
     1441 tattagcgat ccagatcttg ccaaaatgca aaaatttgtt gaaaaattac cgcctgatca
     1501 agtaagcccg gaatggtctt gggttgatag ggttgatatt accagtgttg gtaacagcaa
     1561 aggggcaaga ttagcggaag tattaaaaac agaaggtatt gatttacaaa aggtgattgc
     1621 ctttggcgat aatttcaatg atgtttctat gctagaaacc gttggattag gcattgcaat
     1681 gggaaattca gaaccagata ttcagcaaaa agcgaagaaa actataggtt caaataatga
     1741 agatagtatt gctaatgaat tgaaagctca atttaatctt taattatgat aaaagcaaaa
     1801 tactctattg ctaggcggat aggactctat tttttaatta tgatcggatt tgcttcgatt
     1861 atcagtggaa tctcacttgg tattatgtgg agcaataaat ctgatgcgag cctgattaat
     1921 gtttcaggct cacttcgtat gcaaagttat cgattgttat cagaaatgga ttcaaaaaaa
     1981 gcgctgctac ctgagcgatt gttggaatat catcgaacgc tacatgctac agagcttagt
     2041 tcattaaagc aaagtgtttt attgccagat agcgtgatag aaagctatca acaattactt
     2101 aaagattggt atgaaatgca atcttatgtc gctcagggag ataaagttaa ttatgcttcg
     2161 cacattgaag attatgtgca gagagttgat gattttgttt tccgcttaca agagttcgct
     2221 gagttaaaat taaagattgc aacaggcgtg attgcgattg caatgttact gattattgcc
     2281 cttgcttata ttggcgtttg gtatactcga aaaaagatta ttggaccttt aaggcaattg
     2341 gtaaaagcga gttggcaaat tcaaaataaa gattttgacc acgtaaaatt agcggttaat
     2401 gaacccaatg aattagggct actttcctct acttttactg agatgtctaa tgaattatta
     2461 aaattatata cttcattaga agataaagtt caggataaaa cacgtcgttt attggcggta
     2521 aatcgttctt tactggtatt atatcaatgc tctcaattat taactgctaa gccgattaat
     2581 aaagaagtgt taaatcaagt acttaaaacg gtatttgata atgaacattt gctagggatt
     2641 gatttacaag tatatggggc tgaatattgg aatgtatcaa tagacaataa accaagttta
     2701 gattgggttt ctgctgaaat tgcaatagaa aatgaaaaat tgggtatctt aagctggaaa
     2761 ccctcactgg tttgtcctgg tgaacgattg atccaaaatg tgtcggaaat gattggtcgt
     2821 agcctttatg tagtgcagat gcaaaagcaa cagcaacaat tggtactcat ggaggagcgt
     2881 gcaattattg ctcgtgagtt acacgattcc ttagcccaat ctcttacgtt tttcaaaatt
     2941 caggttagcc tgcttaagct tgctaaaaca gatgaaaagc gtaatgaaat tttaactgta
     3001 tttgaaaaag cgttaaatga tgcttacagc caattaaggg agctgttgac gacgtttagg
     3061 ctaacgattc aagaagcgaa tttacaacaa gcgttagaaa aaattttaga aagcttaaga
     3121 ccgaaaacct cagcacagat tcgcttaagt tgcaaattac cttcgcatat gtttaatgct
     3181 cagcagcaag ttcacgtatt gcaaattgta cgagaagctg tgattaatgc tattaagcat
     3241 tcgcaagcca ctgaaattaa tgtgattgct gaaacaaatg atgatggtga acattgtttg
     3301 actattcaag ataatggcgt agggcttgct gcccatacgc aaccagaagg gcattatgga
     3361 ctgacgatta tgcaagaacg ggcggctgaa ctcaaagccg aattttcgat tgaaaatgtg
     3421 gaaagtggcg gagtaaaagt acaggtttta ttgccgagta tgattagcaa gcggtaataa
     3481 aatattaatt ttttgcgtat tattggcaac gaataaaagg ataacaaatg caattccgaa
     3541 aagcagtaga agcggatttt gagacaatat tggcaattta taatcaagca attcccactc
     3601 accaaattac cgccgattta gaactggcaa ctacgcaaaa tcggcggaag tggttcgatt
     3661 ttcaccttga aacagcccaa tatcctattt gggtggtcga agatgaaaaa ggcattgctg
     3721 gctggtttag tttttcacca ttttatgaaa gaccggcatt tttacatact tcggaaatca
     3781 gtatctattt agaccgctat tccaaaggaa aaggctatgg ctcaaaaatt attgagttta
     3841 tgcaagctga aatgcttaat cacaatattc atacgttgat ggcgtatgtg tttgaattaa
     3901 atacagtgag ccaaaatcta atgagaaaac acggctttga acaatgggga agatttccga
     3961 atattgctaa tatgggaaaa gatgagaaag gtgaggataa atggcgaacg ttgctgatga
     4021 tgtcttacca aaaaggtatc gaataacaag cggtgatttt ttagaatatt tttgcaaata
     4081 aggacaatat ggaaaagaca aaaattgtac ttgcaacaag taatgcagat aaagttaaag
     4141 aaatggcaga tgtactctca caatttggtt ttgaggtggt ggctcaaagt gaatttggga
     4201 ttgaatcacc ggaagaaacc ggtttgactt ttgttgaaaa tgcgctgatt aaagcacgat
     4261 ttgctgctaa aatgacaggt ttaccggcga ttgccgatga ttcaggttta tcggttatgg
     4321 cacttggtgg tgaaccgggg ctatattctg cacgttatgc cggagagcag gcaaccgatg
     4381 ccgataatcg ccaaaaatta ctagccaaaa tgcaagggca agagaatcgt ctggcgaaat
     4441 ttgtcagttg tatcgtgttt ttaaaacacg aaactgaccc aacaccttat attgcgtcgg
     4501 gtgaatgttt cggtgaaatt ttgacggaag aaagaggtga aaatggtttc ggctacgatt
     4561 ccctgttttt ctacccaccg aaagcttgca cttttgccga attagaaacc aaagagaaaa
     4621 aagcgatttc acaccgtgca atcgcactag agcaattaaa acagcaatta gcaggagaga
     4681 ataaatgatt attacaacta cacctacgat agagggtaaa caaattgtag aatacaaagg
     4741 gttagtattt ggcgaagtag tttctggggc taattttatc cgtgatttct tagccggtat
     4801 caccgatatt attggcggac gttcaggtgc ttatgaaagc aaattaaacg gtgcaagaaa
     4861 agaggcatta gctgaattag ctaaggaggc acaaaaagtg ggggcaaatg cggtggttgg
     4921 cgtttctatg gagtatcaat caatgggcgg cgataaagga atgttcatcg tggtagcgac
     4981 tggtactgcg gttgtaatac gttaatttat gcagtttgaa caacactttg aagcaaattt
     5041 ctggcagaac aaatcgctac ttgaaatgaa tgatgctgaa tgggaagcat tatgtgacgg
     5101 ttgtggtaag tgctgctacc ggaaatatat tcagggaaga gggcggcgtg aacggcttta
     5161 ctatacctcg gttgcctgta atttattaga tgtgaataca ggccgttgta tgaattactc
     5221 gaaccgtttt aaaattgagt cggattgtac aaaattaact aaaaagaact tacctgattt
     5281 tggctggttg ccaaaaacct gtgcttatcg tttactttat gaaggaaagc ctctttttga
     5341 ttggcaccct ttaatctcta aagatcctga ttctgtaaaa aatgccggaa tgctgattgc
     5401 taacggtatt cacgaaaaag atgtgattga ttggtttgag tttgttattg atgaagtatg
     5461 agaaaaaggc gtttggtgca aacgcctttt tgttatagag attaggcttt ttccatctca
     5521 aattcaatta acatcatcaa aatatgaatc actttaatgt gaatttcttg gatgcggtct
     5581 gcgtaacgga aatgaggtac tcggatttcc acatcggcta atcctgccat ttgcccgcca
     5641 tctttgccgg tcattgcaat cactttcatg cctttttctt tggcaacttt gatcgcattt
     5701 aacacatttt tagagttacc agaggttgat agaccaaaga gtacatcgcc tttttgccca
     5761 acggcctcta gataacgaga gaagacatat tcataaccga agtcattact tacacagctt
     5821 aaatggctga catcggaaat agcgattgcc gggtatccgg gacggttttc acggtaacga
     5881 cctgttaatt cttcggcaaa gtgcatcgca tcacagtgcg aaccgccatt accgcaagaa
     5941 atcaccttac caccttgctt gaaactattt gcaatcagca tggcggcatc ttgaattaat
     6001 ttaatatttt tttcatcaga cataaatttg tctaatacat ctgcagcttc ttgaagctca
     6061 gctttaattt gttctaaata catcatttgc tcctttgttt gcaaaacgct tacctttaaa
     6121 catcggcttt accaatgggg gtagcgagta aaaaattcgt tttttatgac cgcttgaggc
     6181 ataaaaagtg caataagttt agaagatcta tgctgtattg taaacctaat aacttaaata
     6241 atctgaatag ggttgtaaac aaaagtttcc aaaagagaaa aatagcggta tgatcgcctc
     6301 acaatccctc tcgcattgtt aactctagta gctaatatca ggctttaagc gtattaaaca
     6361 atgcacaaat ttgctattta taggagacta tatggtcgta aatccagaat cttttgaaca
     6421 gataattgga caatctgtaa agataaaaga ggttgttgaa caagcaaaaa aatttgccaa
     6481 ccttgatgca ccattattga ttcaaggtga gacgggaaca ggaaaagatt tatttgccaa
     6541 atcttgtcac cattttggat ctcgtcgaat gcaaaaattt attgccgtga actgtgccgg
     6601 tttaccggct gaagaagcag aatcggaaat gttcggacat cgtggcaatg gcgttgagtc
     6661 cgtcggtttc tttgaatatg ctcacggtgg tacagtatta ttagactcta tttctgagtt
     6721 atcgctggaa atgcaagcca aattattgcg ctttttaaat gatggctctt ttcgtcgagt
     6781 gggcgaagat caagagattc aagtcaatgt tagggtgatc tgtacttcat tacagccttt
     6841 gtcattatta gttgaaaaag gcaaagtaag agaagatctg tttcatcgtt taaatgttct
     6901 cactttaaat ttaccacctt tgcgagatcg aaaagaggat attccattat tagctgatta
     6961 ctttatcgcc caaattagca atcagttggg ggtgagcaaa ttagagtatg ataatgtttt
     7021 tttgcaagca ttgcagaatt atcactggcg aggcaactta agagagcttt ataacgcact
     7081 ttatcgagct tgtacacttt cgcatagcta ccgtttagcg gtaaaagatc ttcatttacc
     7141 cagagatatg gaggcgatga ttgaagtacg tgaggaggcg acacttgagg aaatgatgag
     7201 ccagtatgaa gcccaattat tgcaccaatt ttatgcagaa tatccgagta cccgaaaatt
     7261 agcacaacgg ctcggacttt cacatactgc ggtggcaaac aaattgcgtc aatatgggtt
     7321 aacaaaaaat ggaaacagta aagatgaata aactagcggt cattttttca gtatgttttg
     7381 caaatttagg gctaaattcg accgcttacg cagcgccaag tattcctcaa gagctacttg
     7441 aaaatagcct tgtgtattgc actacttcat cgggattctc ttttaaccca caaaaatccg
     7501 atgtgagttc gaatatgaat gtggtaacag agcagatcta cgataaatta gttgagttta
     7561 atccgaaaaa aaatcgacta gagcctgctt tagctgaaag ttattcaatc agcgacgatg
     7621 gtttagtgat tacattacgt ttaagaaaaa atgtcgcttt tcatacaact ccatggttta
     7681 ctccgagtcg taaattaaac gcagaagatg tggtgttttc gctaaaacga atgattggta
     7741 aagaaagtga tttacctgaa gtgaaaagtg agcctgaaag tgtattacac caccagcgac
     7801 aaagctatat ttaccaaaaa acagcggggc agatttattt tccttatttt gaaagtgttg
     7861 atttagcgaa taaaattgcc agcattcatt ctccgtcaaa tgacattgta aaaattgaat
     7921 tagtccgtcc tgattcagca ttgttggcgc atttagccag ccaatatgca gttattctgt
     7981 ctaaagaata tgctttacag ctcaatgcag atgataactt agttcaactg gatttattac
     8041 cggtaggaac aggcgtatat caacttgaaa gttatgaaaa taatgattat gtgcgtttaa
     8101 aaccaaatat gaattactgg ggcaaaaaag catatattca aaatatggtc gtagattttt
     8161 ccacagaagc gaccggcagg atggcaaaat ttttaaataa tgaatgtgat gtggttgctt
     8221 ttccggaacc tagccaatta tcagtgttaa ataatcggca gcttattagt agtaaaggtg
     8281 ctaatttggt ttatttagca ttcaatatgc aacggggtat tgggcaggat ttggcattac
     8341 gccaacgtat tgcacgcggc ataaatcgca aacgtattgc agagaaatta ttttatggta
     8401 caggtgaagt agcggataac ctattaccaa cagctttgtg gtcgaaaaaa aataatggaa
     8461 gctaccctta tcaaccacac caaaattcag gtgagcaagc ggttgaaatg catgacagta
     8521 ttataaatcg gtctttagat agcaaaacag agaccgaaga agtaaaccaa aaacagcgct
     8581 taaaactgtg ggtaattgat gaaaaacgag tttataatca acacccatta aaaatggcag
     8641 aaatgatacg tgctgattta gctacgcaaa atttagaggt cgaaattcgc gcagtaagtc
     8701 gagcttattt ggtgcaacag cttgctaata aaagtgcgga ttatgatttg attttaggtg
     8761 gttggttagc aaataatctt gatcctaaca gttttttgga ggccttactc agttgtaaag
     8821 ctgcggaaac ggtgactaat ttatcaaatt ggtgcaatga agattttgat ttttggttac
     8881 aaacagcccg tctaagtgat gatccggcat cagctaattt attgtatgaa tttgcacaaa
     8941 aattattaga agagcaattg cctattctcc ctttaatgaa tgctaatcgt gtattagtca
     9001 taaaagatgg agtgaaacag gcagatattt cccctttcgg ccaggtaaaa ttatcagagt
     9061 tacgtttggg cttggggcat aaaaaaggaa actaattatg ctgattgtct tttttcgaaa
     9121 aatttttcta accttgatta cgttgatttt tttgtcactt atcagtttta atattctgtt
     9181 acgagatcca ttaaaccact tagccgagtt aggctggttt gaggcttatt gttattatgt
     9241 aaaatctctg ctaaatggtg attttggtat tagtttcagt aatggagaac cgctcacagc
     9301 gcaaatttta caggtgttcc cggctacaat tacgctttgt ttatcggctc tcattgtttc
     9361 tttaattatt ggtttgcctc ttggtttttt tgcggctact cagcaaaaaa atatggttgg
     9421 gcgattatta aatacactag gttcattgag tttagccgtg ccggtatttt ggctggcatt
     9481 agtgctaatg tattacgctt ctctcaatca gtgggaagtg tcagcaattg gagaaatcca
     9541 ttcgctttat ccgaaacaga tggtaaccgg ctttttgcta ttagatattt ggttatctga
     9601 tgtaccttat aaattaaaaa tgatgcagag tgctttacat cacttagctt tgccgacatt
     9661 agtcttggct gtgccagcta cattagaagt tatgcatatt actcaagcta gagcaggtta
     9721 tgtaatgaaa caaaattaca ttaaaatagc gaaaacgcgt ggctggacac cgtttagagt
     9781 ttggagaacg catattattt gtaatacttt acctgcatta ttacctatga ttgcccgtac
     9841 ctttatttta atttttgcct tcggtatgtt aattgagaac atatttagct ggggaggaat
     9901 tggacgttgg ctgataaatg cccttgctat tcaagactat aatgcgattt cagcgggagt
     9961 agttgcaatc ggggtgtttg tgttattagt agatatggtt acaggctttg tgcgggtgat
    10021 gttagaccca tcagataaaa aggattggta tggcagttat taaagaaccc gagcaattta
    10081 gagaaaccaa cgcatttaag gcattttggt ttcatttccg acaagataaa cttgcacttg
    10141 gcagttttta tctttttttg ctattactgg ctctcatttt tgttggtagt ttgcttgctc
    10201 cttacaatta taatgaacaa tttgttggtt tagaattaat gcctccctct tgggacgaca
    10261 aaggtcaaat cagccatttt ttaggcactg acgatttagg acgggatatt ttaagccgta
    10321 ttatgtatgg tttttactac acgcttggtt ccgcactgat gattacctta ttaattgcga
    10381 ttatcggcgg tatgatcggg atttatgccg ggctgaaaaa aggacgttca ttttggttga
    10441 ttagccattt gttcgatacc tttttattta tgccgatttt attgattgcg atcatcattg
    10501 caaccttaat ggaagcgagt ttggtcaatg caatgttggc gattttctta gcgatgttgc
    10561 cgcattttat ccataaaatt tatcaagcaa ccgaaaaaca actcaaaaaa gagtacatca
    10621 ttaccttaaa gctagacggt gcaagctgct ggtatttaat tcgagaagtg attattccta
    10681 atcttaccgg cgtggcaatt aaagcacttt cacatatttt tgtgatcgcc atattagata
    10741 ttagtgcgtt aagttttatt atgctggggg ctaaaagccc gacccctgaa tggggagcga
    10801 tgattcgtga atccatggaa ctgatttata tagctccttg gacggtgata ttacctggct
    10861 tagcaattat ttttagtatt tttaccgtta caatacttgg tacacgctta agcgtgattt
    10921 ttgaaaaata ccgtaaagaa tagtatttaa ataggatact gacacataac aagcggtcat
    10981 ttttaagtaa aattttacag attaaggaat agtatggcat tacttgatat tcgccattta
    11041 agtattgaaa ttgatatgcc aaaaggcaga gtaaaaatgg tggataacat caacctgact
    11101 gttgatgagg gcgaaatttg tggcttggtg ggagaatctg gttcgggaaa aagcctcata
    11161 gccaaagtga tttgcggtat gattaaagat gaatggattg tgagtgcaga tcgctttcgc
    11221 tttgacgata ttgaattact gaaactttcg ccagcggaac gccgaaaatt ggtaggcgat
    11281 catatttcaa tggtattcca agatccgctt actagccttg acccaagcca gcaaattggt
    11341 aaacaactga tgcaaaccat tcaatttaga gggaagtggt ggcaacgatt tgggtggaaa
    11401 aagaaaaaag caatcgagct actacaccgt gttggtattc gtgatcatca agatattatg
    11461 aaaagttacc caagcgatat taccgaaggc gaagctcaga aagtgatgat tgcaatggcg
    11521 gtggcaaatc agccccgatt attggtggca gatgagccga ctaatattat ggaatcaacc
    11581 acgcagttgc aggtttatcg cttgctttcc agtatgaatc agaatttggg aacctcaatc
    11641 ttgctggtga gtaatgacat gcgtagcatt caaaattggg ttgattctta caatgtgctt
    11701 tattgtggac aaaccgttga aattgcggat aaagagagca ttcttgaatc gtcttaccac
    11761 ccctatactc gagtgttact caatagttcg ccggactttt ctcagccttt agcctttaaa
    11821 agtagcttaa atacgctacg aggctcagtc ccgatgttac aagaaatgcc gattggttgt
    11881 cgtttaggcc ctcgttgccc ttttgcacaa aaaaaatgta tcaacaaacc gccattagtg
    11941 aaagtaaaac agcacgagtt tgcctgccat tttccgatta actttagaga acaaaatttg
    12001 cttaaaaata aaacggaaaa tacgccgttt gtggtggctg aaatagcgga aaaagaggcg
    12061 ttttaatgtg gttattattg gctaagtgta acgggtaaca ttaaagtatc taatacgaaa
    12121 ctcaagggta aatcgatcac cgcaagcacc gcgaatatac cgccttcttg cattacttgg
    12181 aaatctttta ttacgccggc ataaacacgg taatcctgtt caaccaagct tagtactgtg
    12241 ccacaagcgg tgaggttaaa catcaaatag agtagaaaaa cgagtttaca agcggtcaat
    12301 ttcatcaaaa aatctccaaa tacgctttta ttttacgcta tttttatcgg gttggcgtaa
    12361 aattcgtttt aaaaacagct attttcaggc agaatgtagc cgaatcgaca ttaaatatac
    12421 taagaggatc ttatatgcaa acttacaaca ttgcagtgct tgccggtgac ggcattgggc
    12481 cggaagtgat ggcagaagct atcaaagtgc ttgaagcgac tcaacaaaaa tttggcttta
    12541 aactaaattt taatcaatat aacattggtg gagcagcgat tgatgctcaa ggtaaagcgt
    12601 tacccgaaaa taccttaaaa ggctgtgaag aggcagatgc gattttgttt ggctcggtag
    12661 gtggcccgaa atggacgcat ttactacctg atgagcaacc tgaacgaggt tcgctattac
    12721 cactgcgtaa acatttctcg ctattctgta atttacgccc tgctgcattg tatcaagggc
    12781 tggaaaaatt ctgcccatta cgtgccgata ttgctgctaa aggttttgat atggtggtgg
    12841 tgcgtgaatt aaccggcggg atttattttg gacagccgaa aggccgtgat ggtgaaggtg
    12901 cgcaaacgag agcgtttgac accgaagtat actacaaata tgaaatcgag cgtattaccc
    12961 gtgtggcgtt tgaatctgca atgaaacgta aaaaacacgt aacctctgtg gataaagcca
    13021 atgtgttaca aagctcgatt ttatggcgtg aaaccgtcac ggaagtagct aaagagttcc
    13081 cagaagtcac gcttgagcat atgtatattg ataatgcgac aatgcagcta atcaaagcac
    13141 ctgaatcgtt cgatatttta ttatgctcaa acatcttcgg cgatattatt tccgatgagg
    13201 ctgcaatgat tacaggttct atgggcatgt tgccttcagc cagcctcaat gagcaaggtt
    13261 tcggcttata tgaaccggca ggcggttcag caccggatat tgccggtaaa aatattgcga
    13321 atccgattgc gcaaatttta tcagccgcaa tgatgttgcg ttatagcttt aacttaggtg
    13381 atgctgctga tgcgattgaa acagcgatcc aaaaagccct tgctgatggc taccgtaccg
    13441 ctgatttagc tgatgacagc aaaccgcttt ctacctcaga aatgggcgat attattgcga
    13501 aaaatattct cgcttaaggc tagaagttaa atagcacaag cggtcattat tcgccaaatt
    13561 tttgcaaatt ttccgctaat aatgaccgct tgtttattac tcgttagccg tcaatctctt
    13621 cgcctgtttc ttcccactta aaattcaatg ccgcagagtt gaggcaaaaa cgcaaccctg
    13681 ttggtggtgg gccgtccgga aaaacgtgtc ccatatgcga atcacaattc ccacaacgaa
    13741 tttcgatacg atgacgtcct aaactgtaat catctaagta acgtagtgac gattctgaaa
    13801 tcgtttggta aaaacttggc cagccacaac cggcatcaaa tttagtatct gagtgaaata
    13861 aatcgttatg gcatctggca caacgataaa ttcctacccg atgttcattc aaaaattttc
    13921 cggtaaacgg acgttccgta ccgtggttga ttaaaatatc aacttgttgt tctgtcaggg
    13981 cgtgaatagg tttaatcatc agcaattctc ctaagatagt agcgaagtca ttgtagcttt
    14041 ttttgacctg aaaatcgtta taaatttcaa aatttgacat agatcacaaa aaatttatct
    14101 gttatattgg aagggttctt ttggcttgtc aggaaagtga aaagatgtgc tataattcaa
    14161 gcatctttag tgtagtacat aaagaacaaa ttttgtttaa ctttaatata ggtagaaaaa
    14221 tctatggcaa ttaaaattgg tattaacggc tttggccgta tcggtcgtat cgttttccgt
    14281 gcagcacaac accgtgatga catcgaagtg gtaggtatca acgacttaat cgatgttgat
    14341 tatatggctt atatgctgaa atatgattca actcacggtc gttttgacgg tactgttgaa
    14401 gttaaagacg gtcaattagt agttaacggt aaagcgatcc gcgtaacagc tgagcgtgac
    14461 ccggctaact taaaatggaa tgagatcggt gttgatatcg cagttgaagc aacaggttta
    14521 ttcttagatg atgaaactgc tcgcaaacac atcaccgcag gtgcgaagaa agttgtttta
    14581 accggtccgt ctaaagatgc aacacctatg ttcgtaaacg gcgtaaactt tgatacttac
    14641 gcaggtcaag acatcgtgtc taacgcttct tgtacaacta actgtttagc accattagcg
    14701 aaagttattc acgaaaaatt cggtatcaaa gaaggtttaa tgacaactgt tcacgcaact
    14761 actgcaacgc aaaaaacagt agatggtcca tcagcgaaag actggcgtgg tggtcgtggc
    14821 gcgtcacaaa acatcattcc ttcatcaaca ggtgcagcga aagcagtagg taaggtatta
    14881 cctgcattaa acggtaaatt aaccggtatg gctttccgtg ttccaaccac taacgtttct
    14941 gttgttgatt taactgtaaa cttagaaaaa ccagcaactt atgcagaaat ctgtgctgaa
    15001 atcaaacgtg cttcagaaaa cgaaatgaaa ggcgtattag gctacacaga agatgcagtt
    15061 gtttcaacag acttcaatgg tgcaactgaa acttcagtat ttgatgcagc agcaggtatc
    15121 gcattaactg atactttcgt taaattagta tcttggtacg ataacgaagt tggctactca
    15181 aacaaagtat tagacttagt tgctcacgta t
//
LOCUS       DANLZC010000054        15168 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.054, whole genome shotgun sequence.
ACCESSION   DANLZC010000054 DANLZC010000000
VERSION     DANLZC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15168
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.054"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            280..711
                     /locus_tag="PXE44_001252"
     CDS             280..711
                     /locus_tag="PXE44_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL5588767.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEPKKGNPFYACRIAAL
                     VGESDSPEYRYFDMNVVGKETETLIKRCQKAVSEEKKVLVSFVMADLWFDTFTYSTDS
                     KFHKKGDTGVSVKGRLIRIKMIKIDGEIAYQEKPKSDEQSE"
     gene            729..1721
                     /locus_tag="PXE44_001253"
     CDS             729..1721
                     /locus_tag="PXE44_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5588768.1"
                     /translation="MLCWALLLDVQDLRVTFKTPDGDVTAVNDLTFSLNAGETLGIVG
                     ESGSGKSQTAFALMGLLADNGRISGSAKFNGQEILNLPETQLNRLRAEQIAMIFQDPM
                     TSLNPYMKIGEQLMEVLMLHKGMDHKTAFEESVRMLDAVKMPESRKRMNMYPHEFSGG
                     MRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKSEFNTAIIMITHDLG
                     VVAGICDNVLVMYVGRTMEYGTAEQIFYTPSHPYSIGLIGAVPRVDGQEEELITIPGN
                     PPNLLRLPSGCPFQPRCVHATKQCLKAPALTLLNDQRLRACFVPAEQLIRGNAS"
     gene            1718..2707
                     /gene="oppF"
                     /locus_tag="PXE44_001254"
     CDS             1718..2707
                     /gene="oppF"
                     /locus_tag="PXE44_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDL5588769.1"
                     /translation="MNTEKKVILEVKDLSVHFNIKDTKSLFFAKHQTLKAVNHVSFQL
                     YEGETLGVVGESGCGKSTLARAIIGLVESSGGNILWLGKELTQQSAKEWKTIRSDIQM
                     IFQDPLASLNPRMTIGSIIAEPLKIYQPHLTAEEVKEKVRAMMMKVGLLPNLINRYPH
                     EFSGGQCQRIGIARALIVEPKLIICDEPVSALDVSIQAQVVNLLKSLQREMGLSLIFI
                     AHDLAVVKHISDRVLVMYLGNAVELGDYEQVYHDTKHPYTKALMSAVPIPDPQLERNK
                     QIQLLEGDLPSPINPPSGCVFRTRCPLADQHCIADKPLLKGRDNHVVACFKAE"
     gene            complement(2811..3569)
                     /locus_tag="PXE44_001255"
                     /pseudo
     CDS             complement(2811..3569)
                     /locus_tag="PXE44_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            complement(3699..4340)
                     /gene="bioD"
                     /locus_tag="PXE44_001256"
     CDS             complement(3699..4340)
                     /gene="bioD"
                     /locus_tag="PXE44_001256"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDL5588770.1"
                     /translation="MAKVFFITGIDTDIGKTIATGWYAKKLMQQGASVITQKMIQTGC
                     RGIAEDLLIHRKIQGIELTEEDKQGITCPYVFDYPCSPHLAAKLEPRTIERKKIETCT
                     ALLCEKYDYVLLEGAGGLCVPYNEEETTLDYLCQHQYPVILVTSGKLGSINHTLLSLQ
                     VLNSKRVSVHAVIYNLYPETDQVISQETQHFLRRYLEKYSPNTLFEIMDLISV"
     gene            complement(4358..5113)
                     /gene="bioC"
                     /locus_tag="PXE44_001257"
     CDS             complement(4358..5113)
                     /gene="bioC"
                     /locus_tag="PXE44_001257"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDL5588771.1"
                     /translation="MDRIDKGRIKQRFSAAVSNYDQQARAQQQIHQRLLSLLSYIDRQ
                     AFQRALEIGCGTGGLTKQLMSQLQVEHWELNDLCDMREHLQSVLPQPFHFHCGDAEQL
                     PFPQQYDLIASASVVQWFEDKAGFIARCKVRLKKHGFLLFSTFTPENLTQIKHLTGVG
                     LTYPRLEDWQKWLVPEFEILALEHEEIVLHFDDPLAVLKHLKQTGVTATQQQRWTKGQ
                     LHAFCQQYQQHYRNAQHKVELTYTPMYVLARLT"
     gene            complement(5117..5764)
                     /locus_tag="PXE44_001258"
     CDS             complement(5117..5764)
                     /locus_tag="PXE44_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HDL5588772.1"
                     /translation="MKTQHISQQGDNLIVYFAGWGTPPSVVEHLILPENYDLCLCYDY
                     RDLHCDVDFSAYQHIRVVAWSMGVWVAERVMQGYPLLSATAINGTGLPCDDQYGIPEA
                     IFAGTLSGLNEDNRTKFERRICGSKTLLRDYQHYSMRPTLQEIHAELTALYTFLQQDH
                     RTDLISWSKAIVGSKDKIFPAQNQRAYWQDRCAITEIAGEHLLFPHFTHWETCWL"
     gene            complement(5761..6924)
                     /gene="bioF"
                     /locus_tag="PXE44_001259"
     CDS             complement(5761..6924)
                     /gene="bioF"
                     /locus_tag="PXE44_001259"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDL5588773.1"
                     /translation="MIRYYQQQLADLKAQNQFRQLPQLIHRGRFIQREDNTMLNMSSN
                     DYLGLANNEALRQAFFTQYQDQLPALTSSSSRLLTGSFPIYDELESLMAQAFGRETAL
                     LFNSGYHANIGILPALADKKTLIVADKLVHASMIDGIRLAQCEFVRFRHHDYVHLEQI
                     LQKNDRTFERIIVVTESVFSMDGDCADLTQLVALKQRYPQVMLYVDEAHAIGVLGEKG
                     LGLAEQQGCINQIDILVGTFGKALGSMGAYVICDQVIRDYLVNKMRPLIFSTALPPFN
                     VAWTHFVFQQLPHLQAERAHLAQLSQHLRQAIVDIFQVPMPSESCIVPYILGDNELTV
                     RTAQRLQQQGYYCLPIRPPTVPKGTSRIRFSLTADMQVAEVEQFIACLRELAE"
     gene            complement(6921..8222)
                     /locus_tag="PXE44_001260"
     CDS             complement(6921..8222)
                     /locus_tag="PXE44_001260"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDL5588774.1"
                     /translation="MNEQQLLECDRQHIWHPYAALNADFPLFAVERAEGVYIQLKNGQ
                     QLIDGMSSWWSAIHGYNHPRLNHAATQQLSQMSHVMFGGLTHQPAVELAQQLVNILPE
                     SLDKIFFADSGSVAVEVAMKMAVQYQHAKGETQRYKFATIRSGYHGDTWHAMSVCDPV
                     TGMHSLFAKGLPVQYFLPQPPVKFGQAWQPEAMQPLQDLLAQHGDEIAGLILEPVVQG
                     AGGMYFYSPEYLVQAKALCEPYGILLIFDEIATGFGRTGKLFACEHANVVPDVMCIGK
                     ALTGGYLTLSATITNQAVADVICSGEAKCFMHGPTFMANPLACAVAAESIKLLLASPW
                     QQRIQQIEQQLRQELRQAEALDCVAEMRVLGAIGVIEMKAPVNMASLPPRFVQNGVWV
                     RPFGKLVYLMPPYIISSDQLSQLIHGTIRTLQEEYAHKESL"
     gene            8364..9251
                     /gene="psd"
                     /locus_tag="PXE44_001261"
     CDS             8364..9251
                     /gene="psd"
                     /locus_tag="PXE44_001261"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755522.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL5588775.1"
                     /translation="MNILEKKAQLSYWQRIKIAFQYVMPQLYLTRLAGWFAKQQWGAV
                     THFVIKLFAKKYHVDMSEAAKPNFSDYASFNEFFIRPLADNARPINQNPTALCLPADG
                     RISQLGHIEQDLLLQAKGHYFSLNDLLAGDEALAHHFKDGEFATTYLSPRDYHRVHMP
                     CDATLCKMIYVPGDLFSVNPFLAEHVPNLFARNERVICVFDTEFGKMVQILVGATITA
                     SMSTVWAGVINPPRPEKITTWTYEGESAVKLLKGQEMGAFQLGSTVINLFEKDRVQLA
                     SHLQVDSPVRMGEILAHQK"
     gene            9276..9830
                     /locus_tag="PXE44_001262"
     CDS             9276..9830
                     /locus_tag="PXE44_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5588776.1"
                     /translation="MSQQFFDQVSLESQLEVSAEAVAKGIFDVLNQNPPALELNEVSH
                     ALQTLQQQAKFKEIEAVGAQFLAENAQKAGVNTTESGLQYEVLVAGEGQIPAREDKVR
                     VHYTGTLIDGTVFDSSVKRGQPAEFPVNGVIAGWIEALSMMPVGSKWRLTIPHNLAYG
                     ERGAGASIPPFSTLVFEVELLAIL"
     gene            complement(9890..10213)
                     /locus_tag="PXE44_001263"
     CDS             complement(9890..10213)
                     /locus_tag="PXE44_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588777.1"
                     /translation="MNWKAVLIFSVLGSLVSCASYSEHAVQRIEAGKSFAVTGNTKRI
                     NTMACQDNDDWYLDGYRVGKSFREHQQKMLIQRTAYCEEQTGKAVPDKFRHSWNSGYQ
                     QGLKR"
     gene            complement(10237..11340)
                     /gene="proB"
                     /locus_tag="PXE44_001264"
     CDS             complement(10237..11340)
                     /gene="proB"
                     /locus_tag="PXE44_001264"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL5588778.1"
                     /translation="MKIDKTLVVKFGTSTLTQGSAKLNLPHMIDIVRQLAQLHQAGFR
                     LVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQSQLIQTWEKLFAIYDIHIGQIL
                     LTRADIEDRERFLNARDTLRALLDNQIIPVINENDAVATSEIKVGDNDNLSALVAILV
                     QAEQLYLLTDQQGLFDRDPRKHPDAKLIADVDKITDHIRAIAGGSGTSLGTGGMSTKI
                     SAADVATRSGIETIIAPGNRPNVIVDLAYGQAIGTKFSVQADRLESRKQWLYAAPSAG
                     IITIDAGAENAMLIQHKSLLPAGIVNVEGRFSRGEVVKIRTQQGKDVALGMPRYNSDA
                     LHLIQGKHSQDIEQVLGYEYGAVAVHRDDMIVL"
     gene            11506..12494
                     /locus_tag="PXE44_001265"
                     /pseudo
     CDS             11506..12494
                     /locus_tag="PXE44_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
     gene            12569..13216
                     /locus_tag="PXE44_001266"
     CDS             12569..13216
                     /locus_tag="PXE44_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDL5588779.1"
                     /translation="MTINTYAKFAPTVFVAKCSESHTKGEIITLTSKYGNETEVQVHN
                     LVKQDGEHYFYSFTRCDGMDSQSFAQQKAERYQGYADNAAKRSQDWVNAANEGCDFLS
                     LGEPIKIGHHSEKRHRNLIERNARRMDNAVAEMNKAASYESRIAYWEKMAGKIDLSMP
                     ESLEYFKFELDKAKTKHKELKDNPEKREHGYSLTYAKKAVNELEKKVKLAELLWA"
     gene            13240..13647
                     /locus_tag="PXE44_001267"
     CDS             13240..13647
                     /locus_tag="PXE44_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL5588780.1"
                     /translation="MNRQGIYINGKQVFDSQGEDIQSIGFFGCNIQTVVGINKGSILV
                     SSNSNQLLVNGQSIELGSEPVLKIEVLGNVQKLSAGAADVSITGDAGSVDTVSGDVSI
                     KGNVASYVKTVSGDVRCRAITGSVETVSGDIYR"
     gene            13745..14095
                     /locus_tag="PXE44_001268"
     CDS             13745..14095
                     /locus_tag="PXE44_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588781.1"
                     /translation="MTTINLSQQERELVEFVWNNRNTPWFFHCSVSNVSRNNTSRQIA
                     VCVIKDNQLISLDELIAKLCGYKLRQNGLYVTVHNMDAVFDTLYRFMCKLNDEMEANE
                     NVCPYLSLAHYEVF"
     gene            14186..14548
                     /locus_tag="PXE44_001269"
     CDS             14186..14548
                     /locus_tag="PXE44_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588782.1"
                     /translation="MIDTFFDLDDTSCYLGNWEDILKEYDDCLSVEDFWHSAKEFDTV
                     PHFGNLYQERVILKLIARFCIELNIEQDSGLVEFDYYINAIDTHFYINSQRICDIDDW
                     NEMLDKIREEMTPAKLVA"
     gene            14661..14921
                     /locus_tag="PXE44_001270"
     CDS             14661..14921
                     /locus_tag="PXE44_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588783.1"
                     /translation="MTFELGRVCATRAVAECFNQHTLVDLLHRYCSGDWGNLCDNDKK
                     ANVKAIERGGRILARYDVEDETLYVITEADRSYTTILFPYEY"
BASE COUNT         4397 a         3261 c         3125 g         4385 t
ORIGIN      
        1 ctaaattatc ctcaatttaa gacgctgtat ataacagcac ttaataagtc gcataactcc
       61 tgagaagcga cggctatcct tagaggtagt tgagttattg ccaaaatgtc tcctagcatt
      121 ctgaaaggga acgccacttt tgtgggaatg ccacctaaac cctctaccat ctcagtgaag
      181 catcattgag atttcatttt acttccacct cgcggggaac atttttcccg tgttgggcaa
      241 ggatgtttct ccgctttttt acatttggag aaacaaacta tgacaactca aacttctact
      301 caaaaaaatt acttcaacct tcatacaaca ggcattggct atctaaacga tattcgtttg
      361 gtagagccta aaaaaggaaa tcctttttat gcctgccgta tagcagctct ggttggcgaa
      421 tctgactctc ctgagtacag atattttgat atgaatgtag tcggtaaaga gactgaaaca
      481 cttatcaaaa gatgccaaaa agcagttagt gaagagaaaa aagtgcttgt gtcatttgta
      541 atggctgatt tgtggttcga tacatttact tactcaacag atagtaaatt ccacaaaaaa
      601 ggcgataccg gtgtatctgt aaaaggtcgc ttaatccgca tcaaaatgat taaaattgat
      661 ggggaaatag cctaccaaga aaagccaaaa tcagatgagc aatctgagta attttaccta
      721 agcccgacat tttatgttgg gctttattac tcgatgtaca agatttgcgc gtaactttta
      781 agaccccaga tggcgatgtt actgccgtca atgatctgac tttttcttta aatgccggtg
      841 aaaccctcgg cattgtcggc gaatctggct caggaaaatc gcaaaccgct tttgctctaa
      901 tggggctatt agcagataac gggcgaattt caggatcagc aaaatttaat ggacaagaaa
      961 ttctcaactt gcctgaaaca caacttaacc gcttgcgtgc tgaacaaatt gcgatgatct
     1021 tccaagatcc aatgacctcg ctgaaccctt atatgaaaat cggcgaacag ctaatggaag
     1081 tactgatgtt gcataaaggc atggatcata aaactgcgtt tgaagaatcg gtacggatgt
     1141 tagatgcggt gaaaatgccg gagtcacgta aacgtatgaa tatgtatccg catgaatttt
     1201 ccggcggtat gcgtcagcgt gtgatgattg cgatggcatt actttgccgt ccgaaactcc
     1261 tcattgcgga tgaacccaca actgcgctcg atgtgacggt gcaagctcaa atcatgacct
     1321 tattaaacga attaaaaagc gagtttaata ccgccattat catgattact cacgatctcg
     1381 gtgtggtcgc aggaatttgc gataatgtgt tggtgatgta tgtaggcaga accatggaat
     1441 acggcaccgc tgagcaaatt ttctataccc caagccaccc ttattctatc ggcttaattg
     1501 gtgcagtgcc tcgcgttgac ggacaggaag aagaactcat tactattccg ggtaatccac
     1561 ctaacttatt acgcttaccg agtggctgtc ctttccaacc acgttgcgtt catgcaacta
     1621 aacagtgcct caaagcaccg gcgttaacct tgttaaatga tcaacgttta cgcgcttgtt
     1681 ttgtccctgc cgagcaatta atccgaggta acgcatcatg aacacagaga aaaaagtgat
     1741 cctagaagtg aaggatttaa gcgttcattt caacatcaaa gatacgaaat ctttattctt
     1801 tgctaaacac caaactttaa aggccgtcaa tcatgtctct tttcagttat acgaaggcga
     1861 aaccttaggt gtggtcggtg aatcgggctg tggaaaatca actctcgcac gcgcgattat
     1921 tggcttagtg gaaagctctg gcggtaatat tttatggcta ggtaaggagt taactcaaca
     1981 aagtgcgaaa gaatggaaaa cgatccgtag cgatattcaa atgattttcc aagatccttt
     2041 agcctccctg aatccacgga tgaccattgg cagtatcatt gccgaaccat taaaaattta
     2101 ccaaccccat ttaaccgcgg aagaagtgaa agaaaaagta cgcgcgatga tgatgaaagt
     2161 gggcttatta cctaacttaa tcaatcgcta tccacatgaa ttttcgggtg gacaatgtca
     2221 gcgtatcggg attgcgcgcg ccttgattgt ggaaccaaaa ctcattattt gcgatgagcc
     2281 agtgtccgcc ttagatgttt caatccaagc acaagtagtg aatttattga aatctttaca
     2341 acgcgaaatg gggctatcat tgattttcat tgcacacgac cttgctgtgg tgaaacacat
     2401 ttccgatcgt gttttggtca tgtatcttgg taacgcggtg gaattaggtg actatgaaca
     2461 agtctatcac gatactaagc atccttatac caaagctttg atgtctgcgg taccgattcc
     2521 tgatccgcaa ttagaacgca ataaacaaat tcaattgctt gaaggcgatc tcccttcccc
     2581 gattaatccg ccgtcgggct gtgtgttccg cacccgttgc ccactggcag atcaacactg
     2641 tattgcagac aaaccgctcc tcaaaggacg agataatcac gttgttgctt gttttaaagc
     2701 cgagtaatac acacaaccgc acaaaaaagt gcggttgttt ttttgcgaat attcacagtc
     2761 agactttcgt gatactttcg ctctgagata aggattacga tacagagaag ttattcaaca
     2821 atcacacgtg cattgatcgg ttgcactgga cgattattgt gtcctttcat caaggtcgca
     2881 aacgcctcaa cttgagattg gctaatgttg tcatagtgtt ttaacactaa ccaatttaca
     2941 ccttcagaac aaggtggtgt agttaatgac ccgctgaaac gatagtaatc gcgttttttc
     3001 ggaattaatg ttgctgggtc aaaagcggta ctaagcgtat atttctcccc tgctttttgc
     3061 gggaagtttt gccaaattgg ggctaaccat tggttttcac ttgataatac atagagcacg
     3121 ccaagcacag caagattccc ttcattgtct gcgtgtacaa aatgtgcttc catcgggaag
     3181 gatttacctc aaattaagtt ttcactcggc acatggaaat ggaattgttt aagggtaaaa
     3241 gtctttccat taagctgcat gcgattactt gcctctttga aatccacttg aatagtatgc
     3301 ccattattga caatctccac attggaaggt cgataatcca gcttaatcgg cgctaactta
     3361 ccatcaatcg tatgcttgat atctattggt gtttggtttt taccttcgcc acaaatacgg
     3421 aatttgggtg aaagttcagc ccaatgttct ggcgattcgt gtccggtgta tccccaatgc
     3481 gtgtgcgttt cttctttctt aagggggctt gtacacgctg tcaatgctaa tgcacctaac
     3541 gtaagcacaa aaaggtttga ttttttcata gcctagctcc tgattaaaag agatgaattg
     3601 atgagacaac ctaaactcaa tgatgcctta atccgagacg ctttcatctt aggcaaaaca
     3661 acactccatt tcaagtataa cttgctcaag aatatatctt agaccgaaat caaatccatt
     3721 atttcaaata aggtattagg cgagtatttc tctaaataac gacgtaaaaa atgttgtgtt
     3781 tcttggctaa tgacttgatc tgtttccgga tacaagttgt atatcacagc atgcacactt
     3841 actcttttgg agttgagtac ttgtaaactc aataaggtat gattaatact acctagcttg
     3901 ccagaggtaa ctaaaatcac ggggtattga tgctgacaaa gataatcaag ggtggtttct
     3961 tcctcattat aaggtacaca caagccgccc gccccttcca ataatacata atcatatttt
     4021 tcacacaaaa gtgcggtgca ggtttcaatt tttttacgtt cgatcgtgcg cggttctaat
     4081 ttcgcggcta aatgcggtga gcaaggataa tcaaacacgt aaggacaagt gataccttgc
     4141 ttatcttctt ctgtaagctc aataccttgt atttttcggt gtatgagtaa atcttcggca
     4201 attcctcggc agcctgtttg gatcatcttt tgcgtgatga cacttgcgcc ttgttgcatc
     4261 agtttcttgg cgtaccaacc tgtggcgatg gttttgccaa tatcggtgtc gatgccagtg
     4321 ataaaaaaaa cctttgccat actttttcct tctaagatta agttaaacgc gccagcacat
     4381 acatcggcgt gtaagtgagt tctactttgt gttgtgcgtt acggtaatgt tgttgatatt
     4441 gttggcaaaa ggcgtgcaat tgcccttttg tccacctttg ctgctgtgtg gcagtaacgc
     4501 ctgtttgttt caaatgtttt aacaccgcta agggatcatc aaagtgcaac acaatctcct
     4561 cgtgttcgag ggctaaaatc tcaaattcag gcactagcca cttttgccag tcctcaagac
     4621 gtggataagt taaccccaca ccggtgaggt gtttgatctg tgttaaattc tcgggcgtga
     4681 aagtactgaa taacaaaaat ccgtgctttt ttaagcgcac tttacaacgc gcaataaaac
     4741 ccgctttatc ttcaaaccat tgcaccacgg aagcactggc aatcaaatca tactgctgcg
     4801 gaaagggaag ttgttccgca tcgccacaat ggaagtgaaa aggttggggc aacactgatt
     4861 gcagatgctc tcgcatatcg caaaggtcat tcagctccca gtgttcaacc tgcaactgtg
     4921 acattaattg cttcgttaat ccaccagtac cacatccaat ctctaatgca cgttggaatg
     4981 cttgtctgtc gatatacgac aataaactaa gcaaacgttg gtgaatctgc tgttgtgctc
     5041 tcgcttgctg atcatagttc gatacagcgg cagaaaaacg ctgtttaatg cgtcctttat
     5101 caatacgatc catgtgctac aaccaacaag tttcccaatg ggtgaagtga ggaaacaaca
     5161 aatgctcccc tgcgatttct gttattgcac aacgatcctg ccaataagcc cgctgatttt
     5221 gtgcagggaa aattttatct tttgagccga caatcgcctt gctccacgag attaaatctg
     5281 tacggtgatc ttgttgtaaa aaggtataaa gtgcggtcag ttcagcatga atttcctgta
     5341 atgtgggacg catggaatag tgctgataat ctcttaacag cgtttttgaa ccacaaatac
     5401 gacgttcaaa tttagtccga ttatcttcat ttaaaccaga taacgtgcca gcaaaaatgg
     5461 cttcgggaat accatattgg tcatcacaag gtaagcccgt gccattgatc gccgtagcag
     5521 atagcagtgg ataaccttgc atcacacgct ccgctaccca cacgcccatt gaccatgcca
     5581 ccacgcgaat atgttgataa gcggaaaaat caacatcgca atgcagatct cgataatcat
     5641 agcaaaggca taaatcataa ttttccggca gaatcaggtg ctcgaccacg ctcggcggtg
     5701 tgccccaccc tgcaaaatag acgatcagat tgtcgccttg ctgtgaaata tgttgtgttt
     5761 tcattcagca agctcccgta aacaagcaat aaattgttcc acttccgcca cttgcatatc
     5821 tgcagtcagt gaaaaacgaa tacgagaggt tccttttggg actgtcggcg ggcgaatcgg
     5881 taagcaataa tagccttgtt gttgcaaacg ttgcgccgtt cttactgtca gttcgttatc
     5941 gcccaaaata taaggtacga tacaactttc acttggcatc gggacttgaa aaatgtcaac
     6001 aatcgcttgg cgtaaatgtt gactaagctg agcaagatgt gcgcgttcgg cttgcaaatg
     6061 tggtaattgt tgaaagacaa aatgcgtcca tgccacatta aacggcggaa gtgccgtgga
     6121 aaaaatcaat gggcgcattt tattaaccaa ataatctcgg atcacttgat cgcaaatcac
     6181 ataagccccc atagagccca acgccttgcc aaacgtaccg actaaaatat caatttggtt
     6241 gatacagcct tgttgttctg ctaaccctag ccccttctcc cccaatacgc ctatcgcatg
     6301 ggcttcatct acatataaca tcacttgcgg atagcgctgt tttaatgcga ccaactgggt
     6361 gaggtctgca cagtcaccat ccatactaaa cacggattcc gtgaccacga taatccgctc
     6421 aaaagtgcga tcattttttt gcaaaatttg ttcaaggtgg acgtaatcat gatgacgaaa
     6481 acgcacaaac tcacattgtg ccaagcgaat gccatcaatc atactggcat gtaccagttt
     6541 atccgccaca atcagcgttt ttttgtccgc taaagcaggc aaaatcccaa tattagcatg
     6601 ataaccgcta ttaaatagca aggcagtttc tcgtccaaat gcctgcgcca ttaagctttc
     6661 tagctcatca taaatcggaa aagagcctgt taataaacgg gaggaagaag aagtcaatgc
     6721 agggagttga tcttggtatt gcgtgaaaaa tgcctgacgc aaggcttcgt tattggctaa
     6781 acctaaataa tcattggagg acatattcag catcgtattg tcctcacgtt gaataaaacg
     6841 ccctcggtga attagttggg gcagttggcg aaattgattc tgcgccttta aatcagctaa
     6901 ctgctgctga taatacctga tcatagagat tccttatgtg catattcttc ttgaagggtg
     6961 cgaattgtgc catgaattaa ttgagacaat tgatccgatg agatgatata cggtggcatc
     7021 aaatacacta atttgccaaa tggacgcacc caaacgccat tttgcacaaa gcgtggtggt
     7081 aaacttgcca tattgacagg cgccttcatt tcaatcaccc caatcgctcc aagtactctc
     7141 atttcagcga cacaatctaa ggcttccgct tggcgcaatt cttgacgtaa ttgctgttca
     7201 atctgttgaa tacgttgttg ccaagggctt gccaataaca atttgatact ttctgctgct
     7261 accgcacacg ccagtggatt tgccataaac gtcggaccgt gcataaaaca ttttgcctcg
     7321 ccagaacaaa tcacgtctgc taccgcttgg ttggtaatgg tagcggatag agttaaatac
     7381 ccccccgtta aagctttacc gatacacatc acatccggca cgacattggc atgttcacaa
     7441 gcaaataatt tgcctgtacg cccaaatcct gtggcaattt catcaaaaat cagcaaaatg
     7501 ccgtacggtt cgcataaggc ttttgcttgc accaaatact caggggaata aaaatacata
     7561 ccacccgccc cttgcactac cggttctaaa attaaacccg caatctcatc accatgttgt
     7621 gccaataaat cctgtaacgg ttgcatcgct tcgggttgcc aagcttgccc aaattttacc
     7681 gggggttgcg gtaaaaaata ttgcacgggt agccctttgg caaacaagct gtgcatgccg
     7741 gtcacgggat cacacacgga catcgcatgc caagtatcac catgatagcc cgaacgaatc
     7801 gtagcaaatt tatagcgctg tgtttcgcct ttagcatgtt gatattgcac tgccattttc
     7861 atcgccactt ccacggcgac cgaaccacta tcggcaaaga aaattttatc caaactttcc
     7921 ggcaagatgt tgaccagttg ctgcgccagt tctaccgctg gttggtgcgt taatccacca
     7981 aacatcacat gactcatttg actcagttgt tgcgtggcag cgtggtttaa acgggggtga
     8041 ttatagccat gaatcgctga ccaccacgat gacatgccat caatcaactg ctgaccgttt
     8101 tttaactgga tatacacacc ttcggcacgt tctactgcaa ataagggaaa atccgcattt
     8161 aacgccgcat aagggtgcca aatatgttgt cgatcacatt ctaaaagttg ctgctcattc
     8221 atcgtttcac ctcggttgtg aatcgccctt tcaactggcg taaattcacg caaatactta
     8281 cataaacgga ataacttgtt ataatcgcca agcatttggc ttttcaagcc cattattcta
     8341 acttaaaaag atgatttatt actatgaata tattagaaaa aaaagcacaa ctgagttact
     8401 ggcaacggat taaaattgcc tttcaatatg tgatgccaca actttattta acacgtcttg
     8461 caggctggtt tgcaaaacag caatggggtg ctgtcacaca ttttgtgatt aaattattcg
     8521 cgaaaaaata ccatgtggat atgagtgaag ctgcaaaacc aaatttcagc gactatgcga
     8581 gctttaatga attttttatc cgtccattag cggacaatgc ccgtccaatt aatcaaaatc
     8641 ctaccgcact ttgtttaccg gcagatggtc gtattagtca gttaggacac attgagcagg
     8701 atttattgct acaagccaaa ggacattatt tcagcttgaa cgatttgtta gctggcgacg
     8761 aggcgctcgc tcatcatttt aaagatggcg aatttgccac cacctacctc tcaccacgcg
     8821 attaccaccg tgtgcatatg ccttgtgatg ccaccttatg caaaatgatt tatgtgccag
     8881 gtgacttatt ctcagtcaat ccatttttag ccgaacacgt gccaaactta tttgcacgta
     8941 atgaacgcgt gatctgcgta ttcgataccg aatttggtaa aatggtccaa attttagtcg
     9001 gcgcaaccat taccgcgagt atgagcaccg tgtgggcagg tgtgatcaac ccgcctcgtc
     9061 cagaaaaaat caccacctgg acctatgaag gagaaagtgc ggtcaaatta ctgaaaggac
     9121 aagaaatggg agcctttcaa ttgggctcga cggtgatcaa tttatttgaa aaagatcgtg
     9181 tgcaactggc atctcattta caagtggata gcccggtacg tatgggtgaa attcttgccc
     9241 accaaaaata atttttaacc acaataggaa aaacaatgag tcaacaattt ttcgaccaag
     9301 tgtcattaga aagccaactt gaagtgagcg ccgaagccgt cgcgaaaggg atctttgatg
     9361 tgttaaatca aaatccacca gcacttgagt taaatgaagt gagtcatgcc ctacagactt
     9421 tgcaacaaca agcgaaattc aaagaaattg aagccgttgg tgcacaattc ttagctgaaa
     9481 acgcgcaaaa agcgggggta aatactacgg aatctggttt acagtatgaa gtgttagtgg
     9541 ctggtgaagg gcaaattccg gcacgcgaag acaaagtgcg tgtgcattac actgggacat
     9601 taatcgacgg taccgtcttt gacagctcag taaaacgtgg tcaacctgca gaattcccag
     9661 taaatggcgt gatcgctggt tggattgaag ccttgtccat gatgccagtg ggttcaaaat
     9721 ggcgtttaac cattcctcac aacctcgctt atggcgaacg tggtgctggt gcctccattc
     9781 cgccattcag taccttagtg tttgaagtcg aattattagc cattctttaa agctaaacgc
     9841 tagacataaa aaagtgcggt cattttccgc acttttttat gtctagcgtt tatcgtttca
     9901 agccttgctg atagccgctg ttccacgaat ggcggaactt gtccggcaca gccttgcccg
     9961 tttgttcttc acagtaagcc gttcgttgaa tcaacatttt ttgttggtgt tctcgaaatg
    10021 attttcccac acgatagccg tccaaatacc aatcatcatt atcttgacat gccatggtat
    10081 taatccgttt tgtattcccc gtcactgcaa aagatttgcc tgcctcaata cgttgcaccg
    10141 catgttcaga gtatgaagca cagctcacca gcgaacctag tacactgaat atcaatactg
    10201 ctttccaatt catcttgtta cctttctttt tctgatttat aacacaatca tatcgtctcg
    10261 gtgtaccgca accgccccat attcataacc gagaacctgt tcgatatctt gcgaatgttt
    10321 gccttgaatt aaatggagcg catcgctgtt ataacgtggc atccctaagg caacatcctt
    10381 gccttgctga gtacgaattt ttaccacttc accgcgtgaa aagcgccctt ccacgtttac
    10441 aatcccagca ggcaataacg atttatgttg tattaacatc gcgttttccg cccccgcatc
    10501 aatggtaata ataccggctg acggcgccgc atatagccat tgtttacgac tttctaagcg
    10561 atctgcttgc acactaaatt tggtgccaat ggcttgccca taagctaagt ccacaatcac
    10621 attcgggcga ttacccggtg caataatggt ttcaatgccg gagcgggttg ccacatccgc
    10681 ggcactgatt ttggtggaca tgccacctgt gcctaacgag gtgccacttc cgcccgcaat
    10741 cgcgcgaata tgatcggtaa ttttgtccac gtcggcgatc aatttggcgt ctggatgttt
    10801 gcgcggatca cgatcaaata aaccttgctg atcagtaagc aaatacaact gttctgcttg
    10861 caccaaaatt gccactaacg cggataaatt atcgttatcg cctactttaa tttctgacgt
    10921 agcgacagcg tcattttcat taatcactgg aataatctga ttgtctaata aggcgcgcaa
    10981 agtatctctg gcatttaaaa aacgttcacg atcttcaatg tcggcacggg tcaataaaat
    11041 ctgcccaata tgaatatcat aaatggcaaa caatttttcc cacgtttgaa tgagctgact
    11101 ttgtcctacc gcagccaata attgtttcga ggcaatcgtc ggtggcaatt gcgggtgatt
    11161 taaataatga cgacctgccg caatcgcccc tgaggtaaca atcactaaac gaaagcccgc
    11221 ttgatgaagc tgtgcgagct gacgcacaat atctatcata tgaggtaaat ttaacttcgc
    11281 cgatccttgc gtaagcgtac tggttccaaa ttttacaacg agtgttttgt cgattttcat
    11341 cttctctatc ttcttatttt tgtcgcacaa aattgaagct aacttaccat taatcatcag
    11401 aaaaatcatc tcgtttactt gctattttct atgatttagc acaaattttt tctcactaaa
    11461 aggcatataa taaagcttct tgatttttca gctaggaacc cgattatgac ctcaacctct
    11521 tcacaagaaa cggagcggca acgagaaatc actcgccttt gtgttcaagt tgcccaactc
    11581 ttatacaaca tggtgcagaa agtgccatcg ttgtgcaaat ggcacatcgc ttaggcttag
    11641 ccctcggtgt agatagcgta gaatgtgccc tcacgccaaa tgccattatt cttactacat
    11701 tagcccaaga ccattgcatc acgacagtcc gtaaaaacca agacaaagga attaatatgc
    11761 aagtggttac cgaagtgcaa cgtattgtga ttacagcaga acgtaaaatc tatgatatgc
    11821 aacaagtgca tgccaaactg gctcgcatta aaccgcttaa atacaatcgc tattttgtga
    11881 tgttgatgat tggcgtatcc tgtgccagtt ttgctcattt atctggtggg gacgcactag
    11941 tttcattggt tacatttctc gccgcaacac tcgccatgta tgttcgccaa tcacttgccg
    12001 caagacacta taacccctta attgtatttg cggtgaccgc ctttgtcgcc tcactaattg
    12061 cgggtatcgc gttgaaatat aacatcggca atcagcccca aattgccttg gcatccagcg
    12121 tattattgct cgttcccggc tttccactga ttaatgcact cgccgatatt ttaaaagggc
    12181 atatcaatat ggggattgcc cgttgggcac tcgcgacaat cctcacattt ggggcatgct
    12241 taggcattgt atttgcgctg agtttattct tagaggaaat ctgtatgcta aataaattca
    12301 ttcaagatga atttctacgg ctagatcaac gttatgcagc ttggtatcaa agtgaagaaa
    12361 cggatgaagt tgccagaatg gggattcaag aaagtgagcg gattttgaaa attctgtatg
    12421 cactgaaata taacgaagac cctatgcaaa atggtttagg ctatattgca tttatggcat
    12481 caaaaggcca ataatttact tatctcaaca atttacccac cggggaaact cctcgatggc
    12541 gtttccccta ctttatagga gaaacgctat gactattaac acttatgcaa aatttgcacc
    12601 taccgttttt gttgcaaaat gctctgaatc acataccaaa ggtgaaatta ttacgctaac
    12661 cagcaaatat ggtaatgaaa ccgaggttca agtacataat cttgttaaac aagatggcga
    12721 gcattatttt tattcattta ctcgttgtga tgggatggat agtcagtcat ttgctcaaca
    12781 aaaagcagaa cgctatcaag gctatgcaga taatgcagcc aaacgtagtc aagattgggt
    12841 aaatgccgct aatgaggggt gtgatttttt gagtttaggt gagccaataa aaattggtca
    12901 tcacagcgaa aagagacacc gtaaccttat tgaacgtaat gctcgccgta tggacaatgc
    12961 ggttgcagaa atgaacaaag ctgcaagtta tgaatctcgt atcgcatatt gggaaaaaat
    13021 ggcaggaaaa attgacctgt ctatgccgga aagtctggaa tactttaagt ttgaattgga
    13081 taaagccaaa acaaagcaca aagaattgaa ggacaacccc gagaaacgag agcatggtta
    13141 ttcactgact tatgctaaaa aagcagttaa tgaacttgag aaaaaagtaa aattagctga
    13201 attactttgg gcataattat ttacaaagga gatgacgata tgaatcgaca aggtatttat
    13261 attaatggca aacaggtgtt tgattcacaa ggcgaagata ttcaaagcat tggatttttt
    13321 ggctgcaata ttcaaacggt cgtaggcata aacaaaggaa gtattttagt ctcgtccaat
    13381 agtaatcaat tattggtgaa tggacagtcg attgaattag gttctgagcc agtcctcaaa
    13441 attgaggttt tgggcaatgt gcagaaactg agtgccggtg ccgcagatgt ctctattaca
    13501 ggagatgccg gcagtgttga cacagttagc ggtgatgtct ctattaaggg aaatgttgcc
    13561 tcttatgtga aaaccgtgag tggtgatgtt cgctgtcgtg ctattaccgg ttcggtggaa
    13621 accgtctccg gtgatattta tcgctgatat ttactcatct tattatttac caccctattg
    13681 ggaagcaatt ttcccaaggt gggcagattg tttcccattt ttattttgaa tttggagaaa
    13741 catgatgacc actattaacc tttcacaaca agaacgtgag ttagttgaat ttgtctggaa
    13801 taaccgtaat acaccttggt tttttcactg tagtgtatcg aatgtgtcga gaaataatac
    13861 ctctcggcaa attgctgtat gtgtcattaa ggataatcaa ttgattagcc ttgatgaatt
    13921 gatagcaaaa ttatgtggtt ataaactcag acaaaacgga ttatacgtta ctgttcataa
    13981 tatggatgct gtttttgata ccttatatcg ctttatgtgc aagctaaatg atgagatgga
    14041 ggcaaatgaa aatgtttgcc cttatttatc attagctcac tatgaagtgt tttgattggt
    14101 atcagaacta tccaccctgt cgggaaacaa tttttccgat agggcagatt gtttcccact
    14161 tttattttga atttggagaa acataatgat agacaccttt tttgaccttg acgacacctc
    14221 atgctattta ggtaactggg aggatatact gaaagaatat gatgattgtc tttcagttga
    14281 agatttttgg cattccgcta aagaatttga taccgtacct cattttggca acctatacca
    14341 ggagcgtgtc atccttaagt tgattgctcg attctgtatt gagctgaata ttgagcaaga
    14401 ttcaggttta gtcgaatttg attattatat caatgcaata gatacgcatt tctatattaa
    14461 tagccaaaga atctgtgata tagatgattg gaatgaaatg ttagataaga ttcgtgaaga
    14521 aatgacgccg gctaaattag ttgcctaatt tttgcaaatt tttactaaaa atgacgctta
    14581 ctgtcgtctt aattcaccca cttggggaaa tatctcccca atgggatgac atttcccctc
    14641 tttctgttta ggagaaatgt atgacttttg agcttggtag agtatgtgca actcgtgcag
    14701 ttgctgaatg tttcaatcaa catacattag ttgatctact tcatcgctac tgcagtggtg
    14761 attggggaaa tttgtgtgat aacgacaaga aagccaatgt gaaagctatt gaacgcggag
    14821 gacgcatttt agctcgctat gatgtggaag atgaaacact ttatgtaatc acagaggctg
    14881 atcgtagtta cactacaatt ttatttcctt atgaatatta atggagcttc catttatacc
    14941 ctacattttt atgttatcaa acgccgtcaa atgatggcgt ttttctattt tatatctttc
    15001 gattttatag ttgtttcaat atgcaatttt aattattttt cctctttgta gaacaattac
    15061 aaatgaatta agcatggaaa aacaaatata taacagaaga aaaacattat tcggcaatga
    15121 ggagacggct gcactttatt atgcagctaa agacattatc tttgaata
//
LOCUS       DANLZC010000055        14853 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.055, whole genome shotgun sequence.
ACCESSION   DANLZC010000055 DANLZC010000000
VERSION     DANLZC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14853
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.055"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            28..606
                     /gene="ruvC"
                     /locus_tag="PXE44_001271"
     CDS             28..606
                     /gene="ruvC"
                     /locus_tag="PXE44_001271"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDL5588784.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQNGRQLEYLGSGAIRTTAEDLPTR
                     LKRIYAGVSEIITQFQPNMFAIEQVFMAKNPDSALKLGQARGTAIVAAVNHDLPVFEY
                     AARLVKQTVTGIGSADKVQVQDMVTRMLKLSAKPQADAADALAIAITHAHSIQHSLIV
                     AKQSQQNVSSEKEQILALMKTRYSRGRFRLKG"
     gene            complement(672..1952)
                     /gene="murA"
                     /locus_tag="PXE44_001272"
     CDS             complement(672..1952)
                     /gene="murA"
                     /locus_tag="PXE44_001272"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL5588785.1"
                     /translation="MEKFRVYGPFSLNGTVDISGAKNAALPILFAAILAEKTVTLKNV
                     PDLKDVDTTFKILRQLGVTVEKGSDKGVVHIDASKINNYVAPYELVKTMRASIWALAP
                     LVARFQLGQVSLPGGCTIGARPVDMHISGLEKMGAKIELDEGYVKATSNGRLNGARIY
                     MDKVSVGATLSVMMAGTLAKGTTVIENAAREPEIVDTADFLNAMGAKISGAGTDMITI
                     EGVEHLGGCEHSVVPDRIETGTFLVAAAISGGRITCRNTKADTLDAVIEKLREAGMQV
                     DVTENTITLDSLGRRPKAVNIRTMPHPGFPTDMQAQFTLLNTVAEGTSRITETIFENR
                     FMHIPELIRMGAKAEIEGNIAICYGVEQLKSAEVMATDLRASISLVLAGCIATGETIV
                     DRIYHIDRGYEHIEEKLRQIGARIERFSAPFEGE"
     gene            complement(2062..2322)
                     /locus_tag="PXE44_001273"
     CDS             complement(2062..2322)
                     /locus_tag="PXE44_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL5588786.1"
                     /translation="MNPSQIEEILKQALSDATEVHAQGENAHFGVIVVSDSIASLSKV
                     KQQQAVYAPLAEYFTTNAIHALTIKVFSVEKWKTERLLNMVG"
     gene            complement(2355..2678)
                     /locus_tag="PXE44_001274"
     CDS             complement(2355..2678)
                     /locus_tag="PXE44_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDL5588787.1"
                     /translation="MPQKTLQWDIQQNNESLFVKLSGELTRNTLLPLWKQRASFLSPT
                     GKQSLYWDLKEITRIDSAGFTLLAELLNHYHKIIPNSLINTPDSVRTLAALYDLDGWF
                     EQFII"
     gene            complement(2690..3325)
                     /gene="mlaC"
                     /locus_tag="PXE44_001275"
     CDS             complement(2690..3325)
                     /gene="mlaC"
                     /locus_tag="PXE44_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDL5588788.1"
                     /translation="MLKNIKKIIVTGLVAVSALFSTTAFAETSPYVLMQQTADKLFGD
                     IKANQAKIKSNPEYLRTIVRNDLMPHVHVKYAGQLVLGKNLSAATDAQKEAFFNAFGQ
                     FVEQSYAQVLTQYQDQSVQIESPKSVDGKSIVSIRVNVSGKGAAQPIKLDFKWRKNSK
                     TGEWQAYDMAAEGVSMVATKQNEWAGVIRQKGIDALIAQVAQSAKQPITLK"
     gene            complement(3371..3895)
                     /gene="mlaD"
                     /locus_tag="PXE44_001276"
     CDS             complement(3371..3895)
                     /gene="mlaD"
                     /locus_tag="PXE44_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDL5588789.1"
                     /translation="MRQSIKYEFWVGLFVLLGLAALVFLGLRVANVQGFSNEKTYTLY
                     ATFDNIGGLKVRAPIKVGGVVVGRVADISLDNKTYTPKVALAVNESFNQIPDTSSLSI
                     KTSGLLGEQYVALNVGFVIEGETAMMKEGDTFVDTNSAMVLEDLIGQFLYGDKKSDKT
                     EDEKASDSAEPKAE"
     gene            complement(3916..4692)
                     /gene="mlaE"
                     /locus_tag="PXE44_001277"
     CDS             complement(3916..4692)
                     /gene="mlaE"
                     /locus_tag="PXE44_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDL5588790.1"
                     /translation="MVNFISSIGAVVINFIRTLGRSAFMLWGALIGKPEFRKHTPLLI
                     KQLYVLGVQSLLIIMLSGLFIGMVLGLQGYVVLVDFAAESSLGTLVSLSLLRELGPVV
                     TALLFAGRAGSALTAEIGLMKATEQLSSLEMMAVDPLRRIIAPRFWAGVISMPVLAAI
                     FTAIGIWGGSLVGVDWKGVDGGSFWSVMQNSVNTSDLINGFIKSVIFAFAVVWIALFN
                     GYDCVPTSEGISQATTRTVVNASLVILGLDFILTAIMFGG"
     gene            complement(4685..5488)
                     /gene="mlaF"
                     /locus_tag="PXE44_001278"
     CDS             complement(4685..5488)
                     /gene="mlaF"
                     /locus_tag="PXE44_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDL5588791.1"
                     /translation="MTKTENLVEVKNLTFKRGERVIYDNLNLQVQKGKVTAIMGPSGI
                     GKTTLLRLIGGQILPESGEILFDGRDICKANNKELYEIRQRMGMLFQSGALFTDISTF
                     DNVAFPIREHTRLPEELIRKLVLLKLEAVGLRGAANLMPSELSGGMARRAALARAIAL
                     DPDLIMYDEPFTGQDPISMGVIVELIKELNQALNLTSIVVSHDVKEVLSIADYAYIVA
                     DKRVIAEGTPEILQASEDPQVVQFLAGKADGPVRFHYPAKDYTEELFNG"
     gene            5713..7107
                     /locus_tag="PXE44_001279"
     CDS             5713..7107
                     /locus_tag="PXE44_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HDL5588792.1"
                     /translation="MNKTMKKSFLTAFILGSTIAAMPAYAEATLPTAVNGQAVPSLAP
                     MLEKVRPAVVSIAVEGKTKGDSRRSREIPEEFEFFFGPHADLFGNRSSAPRNFRGVGS
                     GAVINAEKGYVITNNHVIDNADKITVKLEDGREFKAKLVGADPLSDVALIQVEDPKNL
                     TEIKFADSDKLQVGDFTVAIGNPFGLGQTVTSGIISALGRSTGQSDEGYENYIQTDAA
                     VNQGNSGGPLINLNGELIGINTAIISPSGGNAGIAFAIPSNMANSLVTQIIEFGEVKR
                     GMLGIKGGELNADLAKEFNISTQQGAFVSEVLPDSAAAKAGFKAGDVITEINGQKIRS
                     FSELRAKVATSGVGKEIELTYLRDGKEAKAKVTLQSDSEAKVTASNLIPALKGADFNN
                     YNAKGIKGVEISNVEKGSIAEMRSLKKGDIIIGVNRHSVENIADLRKILETKPSVVAL
                     NIVRDGANFYVILQ"
     gene            complement(7159..7920)
                     /locus_tag="PXE44_001280"
     CDS             complement(7159..7920)
                     /locus_tag="PXE44_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HDL5588793.1"
                     /translation="MKTREQLSQENYQQAVKFAQHFADDVRQEFEREAEQRYQFERTQ
                     FEQSVLQNNAQNRPLAKIASLSCVAILLLAMTYYGLSGRYQVVQSGLQQHQDFQQQMS
                     DAHGTSKNERYILSLQNQLRENPNNGDLWYELGQAYALSNDFDSALVCYDNAEKLLGK
                     RAAILGAIATARYYDNGQKMTVEIQAIIEQALQLDKNENASLLLLASDSFLNNNFQQA
                     LDNWRKVLDSNNDSINRRAIIQSMEMARQMLNSQQ"
     gene            complement(7907..8359)
                     /gene="nrfF"
                     /locus_tag="PXE44_001281"
     CDS             complement(7907..8359)
                     /gene="nrfF"
                     /locus_tag="PXE44_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDL5588794.1"
                     /translation="MRNSIISIGFLQKIRKILPLVFGLLLFVGTVQAEMVDTFQFQNE
                     ADRVRAISLARSLRCLQCQNQNLVESNATAAYNMRIEVYEMVNQGKSNEEIIRIMTER
                     FGSFVNYDPPFTPQTWVLWGLPVGLFSLLFLAILWRTRKNKGNKNENA"
     gene            complement(8349..8882)
                     /locus_tag="PXE44_001282"
     CDS             complement(8349..8882)
                     /locus_tag="PXE44_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDL5588795.1"
                     /translation="MKKILLLIPLLLLVVIVGFLTVPLMNKDALAPTEDWQGKPFPEF
                     VGKNLLDSNAHLNNNSLPKEPYILNVWVSWCTWCIKEFPILLELKQKGVPIVGLTYSD
                     RPNDALNALKNWGNPFSLIIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNP
                     DFAQDFLPKLEALRNEK"
     gene            complement(8879..10783)
                     /gene="nrfE"
                     /locus_tag="PXE44_001283"
     CDS             complement(8879..10783)
                     /gene="nrfE"
                     /locus_tag="PXE44_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HDL5588796.1"
                     /translation="MLPELGYFSLLLAAISAIFQVGFALLGEVRKQYHWLALAPLFTY
                     LQAAFTTFSFSTLAYAFLTDDFSVIYVAAHSNSQLPDFFKFAATWGGHEGSMLFWLTA
                     LVIWSAVFCKFSQKIDRLFANRTLAMLGLIALGFLLFILLVSSPFERGFPPPPEGRDL
                     NPMLQDIGLIFHPPLLYLGYVGFAVSFAMIVAALVSGGMDAAIMRWLRPWTMISWGFL
                     TAGIILGAWWAYYELGWGGWWFWDPVENASLMPWLLGTALVHSLIVSQQRGIFYYWTI
                     LLAIFAFALSLLGTFIVRSGVLTSVHAFAVDPDRGMALLILFFGLSFIALALFAFRAN
                     LWQGKVRFNLLSKETAFLLINGLFSVAASVVLLGTFYPMIFTVMNWGSISVGAPYFNS
                     VFTPLALLLMIVMGFAVVLGWKQISNKQILVKLWLLPIAVGLALGLIYASISQREHFA
                     LQPLPTIFISLAIWIILTHLPYSQKMWQIRPLAMRLAHIGFAVCVIGAMMNSYYGDEI
                     GVRLKPNQAAELAGYTFKYEDYHDLIGPNYTAEQAVFSISRDEQLLATVTPERRYYDV
                     RTMTMAEVGLFHHNLDDIYIVMGDKFGNLEYAFRLHYKPYVQALWLGGMMMVMAALLA
                     LFGYRRENKK"
     gene            complement(11053..12000)
                     /gene="nrfD"
                     /locus_tag="PXE44_001284"
     CDS             complement(11053..12000)
                     /gene="nrfD"
                     /locus_tag="PXE44_001284"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDL5588797.1"
                     /translation="MNEYVPFQTPNLVWDGTIAIYLFLLGISSGAVQLAIAYRNSGVK
                     MEKPSQNWIIRSAAILGTLPTIIGLLLLIFHLTKPWTFWKLMFNYNFTSVMSMGVMLF
                     QLYMAVLVVWIAIMFKDWIVRYLPVVSGLIDFAEAKLLKPIEFILFVLAAVLGAYTGF
                     LLSALVSYPMLNNPVLPALFLASGASSGIAATFLVILLAGKLSGESHEVHFMHKFEVP
                     IMVTELGLIVAFFVGLHFGGADKSLALTHALNGFWGNVFWIGVMLIGIAIPLVANIFG
                     SHRLKHNVKFIILISVFDLIGVLCLRYFILYAGQLTVAS"
     gene            complement(12000..12677)
                     /gene="nrfC"
                     /locus_tag="PXE44_001285"
     CDS             complement(12000..12677)
                     /gene="nrfC"
                     /locus_tag="PXE44_001285"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDL5588798.1"
                     /translation="MTCTRRDFVSGAGAIAVVASTGLATSITLATEKEQKKIRYAMVH
                     DETACIGCTACMDACRTTNKVPEGVSRLEIIRSEPFGEFPNVQYEFFRQSCQHCTNAP
                     CVSVCPTGASFIDPETGIIDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRD
                     TNLAEGKQPACVEACPTKALTFGDMNDPSSDIFHKVKNNPVYRTKTALGTEPNLYHIP
                     FGKGEHR"
     gene            complement(12677..13333)
                     /gene="nrfB"
                     /locus_tag="PXE44_001286"
     CDS             complement(12677..13333)
                     /gene="nrfB"
                     /locus_tag="PXE44_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDL5588799.1"
                     /translation="MKKLISKAGRAVALIQAVVFAGFFAISSANALMPEQPKPLWQSE
                     TADDAKVAQDARRDPNKYCTQCHESANATGKPFHHAGKHFQKDVKSPNNGEPLTCISC
                     HGNISESHRKGAKDVMRFNPHGKASNPSLERSVNEQNQVCFACHTAEKLREKFWAHDT
                     HATKIACTNCHEIHPEKDPMKDIPEKDRIKLCTDCHTKIHSGEFKNPEMKATEAKGTK
                     "
     gene            complement(13352..14827)
                     /gene="nrfA"
                     /locus_tag="PXE44_001287"
     CDS             complement(13352..14827)
                     /gene="nrfA"
                     /locus_tag="PXE44_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDL5588800.1"
                     /translation="MATISILGCLQTALAEEAKTESLTDKAVKHEKLGVKIESANHLF
                     AEKYPLQYDSWKSTAKSTDRGSALEADPRYVILWAGYAFAKDYNKPRGHFYAVTDVRD
                     ILRTGAPKDENDGPQPMACWTCKGPDVPRLIEEKGERGYFDPKWAKYGAEIVNSIGCA
                     DCHDTTSEEFKQGKPALRVARPHVLRALNTVGWKFEDLDKHGKRPAVCANCHVEYYFK
                     NKTDVTFPWDKGVDVDSIEKYYDEINFTDWTHALSKAPMLKTQHPDFEVWSQGTHGKN
                     GVTCIDCHMPKVKDKDGKVYTDHKIGNPFDQFEATCKTCHEQSKETLEKRVKEYKHEV
                     KEAMIRLEDQLVKAHFEAKAAWEAGATQEEMKEILMAIRHAQWRWDYSAAGHGNHFHA
                     PDVMLRTIATGIDRAADARAKLGVVLAKHGVTTPVAIPDISTKEKAQLAIGLDIPKEQ
                     AAKDEFLKTVVPQWEKEARAKGLLPAEEADKPVAAPKAEAK"
BASE COUNT         4374 a         3358 c         2926 g         4195 t
ORIGIN      
        1 tcttctcgct tattttattg cacaaagatg gctattattt taggaattga ccccggctca
       61 agggtaacag gttacggtgt gattcgccaa aacgggcgac agctggaata tttaggaagt
      121 ggggctattc gaaccacggc tgaagatctg cctacccgct taaaacggat ttatgcaggc
      181 gtgagtgaaa tcatcactca atttcaacca aatatgttcg ccatagagca ggtatttatg
      241 gcaaaaaacc ctgattctgc cttaaagctc gggcaggctc gaggaacagc gattgtagca
      301 gcggttaatc acgatttacc tgtgtttgaa tatgccgccc gattagtcaa acaaaccgtt
      361 acgggaattg gttctgctga taaagtacaa gtgcaagata tggtaacccg tatgcttaaa
      421 ctctccgcca aaccacaagc cgatgcggct gatgcattag ccattgcgat tacccacgct
      481 cattcaatcc aacattcact gattgttgct aagcaaagcc agcagaatgt gagtagtgag
      541 aaagagcaaa ttttagcact aatgaaaacc cgttatagcc ggggaaggtt taggttaaaa
      601 ggctaactca atataaaatg accgcttgga actaatgttt caagcggtca ttttttgaga
      661 aaattttaca attactcgcc ttcaaaagga gctgagaaac gttcaattct cgccccaatc
      721 tgacgtaatt tttcttcgat gtgttcatag ccacggtcta tgtggtaaat tctatccaca
      781 atcgtttcac ccgttgcaat acagcctgcc aacactaagc tgatggaagc acgcaagtca
      841 gttgccatta cttctgctga ttttaactgc tctacaccat agcaaattgc aatattacct
      901 tcaatttccg ctttggcacc catacgaatc agctcaggaa tatgcataaa acggttttca
      961 aaaatcgttt ctgtaatacg gcttgtgcct tctgcaactg tatttaataa ggtaaattga
     1021 gcttgcatat cagttgggaa acctgggtgt ggcatagtgc gaatgttcac tgctttcggg
     1081 cgacgaccta gagaatcaag cgtgatagta ttttccgtca catcaacctg cattccagct
     1141 tcacgtaatt tttcgatcac tgcatcaaga gtgtctgctt tggtgttacg gcaagtaatt
     1201 cgaccaccgg aaatagccgc tgccactaaa aatgtaccgg tttcaatacg atccggcact
     1261 acgctgtgtt cgcaaccgcc taaatgctca accccttcaa tggtaatcat atcggtacct
     1321 gcaccagaaa ttttagctcc catcgcattt aagaaatcgg cagtatctac aatttcaggc
     1381 tcacgagcag cattttcaat aacagtagta ccttttgcta gagtacctgc catcatcacc
     1441 gataaagttg ctcctacgct cactttatcc atatagattc ttgcaccgtt taaacgaccg
     1501 tttgaagttg ctttgacata gccttcatca agctcgattt tcgcccccat tttttcaaga
     1561 ccggaaatgt gcatatctac cggtcttgcc ccgatagtac aaccgcccgg taaagaaact
     1621 tgcccaagtt ggaaccgggc aactaatgga gctaacgccc aaattgaagc acgcattgtt
     1681 ttcactaatt cgtaaggtgc aacgtaatta ttgatcttac tcgcatcaat atgtaccacg
     1741 cctttatcgc tacctttttc aaccgttacc cctaattggc gtaagatttt aaaagtggtg
     1801 tccacatctt ttaaatcagg cacatttttt aacgtaactg ttttttcagc caaaatagcg
     1861 gcaaataaaa tagggagtgc ggcatttttc gcacctgaaa tatcgacagt accatttaaa
     1921 gaaaatggac catacacacg aaatttttcc atttgggaga tcctaaaaaa taataatttg
     1981 taggggcgaa aaatttttcg cccaattttc caacgtttac aaaagagttt gggcgaatag
     2041 tgattcgccc ctacatttca tctatccaac catatttagc aaacgctcag ttttccattt
     2101 ttccacgctg aatactttaa tggttaaagc gtgaatcgca ttagtagtga aatactcagc
     2161 taaaggggca taaacagctt gctgttgttt tacttttgaa agagaagcaa tgctgtcact
     2221 tactacaata acgccaaagt gggcattctc gccttgagcg tggacttctg ttgcatcaga
     2281 taatgcttgt tttaaaattt cttcaatttg tgatggattc attctgactt cctaaaatat
     2341 gattggtaac tcactcaaat aataaattgc tcaaaccaac cgtctaaatc gtataatgca
     2401 gccaaagttc tgacagaatc tggcgtatta atcaaactgt ttggtatgat tttatggtaa
     2461 tgattcagta attcagcaag taaggtaaaa ccggcagaat caatccgagt gatttctttt
     2521 aaatcccaat ataggctttg tttccctgtt ggagataaaa aagaagcacg ttgcttccat
     2581 aaagggagca acgtgttacg agttaattcg cctgatagtt tcacaaaaag actttcgtta
     2641 ttttgctgaa tgtcccattg taatgttttt tgaggcataa ctaaactact tattttaaag
     2701 tgataggctg ttttgcagat tgtgccactt gtgcaattaa ggcatcaatc cctttttgac
     2761 gaatcacacc cgcccattcg ttttgcttgg ttgcaaccat acttactcct tcagcggcca
     2821 tatcgtatgc ttgccattca cctgttttgc tgtttttacg ccatttaaaa tctaatttga
     2881 tcggctgtgc agcaccttta cctgacacgt ttacacgaat gcttacgata gatttaccat
     2941 ccaccgattt cgggctttca atttgcacac tttgatcttg atactgggtt aacacctgag
     3001 cataagactg ctcaacaaat tgaccaaaag cattaaagaa tgcctctttt tgtgcatcag
     3061 tcgcagccga taagtttttc cctaacacta attggcctgc atatttcacg tgaacgtgtg
     3121 gcattaaatc gttacgcaca atggtacgca aatattcagg gtttgattta attttcgctt
     3181 gatttgcttt aatatcgcca aataatttat ctgcggtttg ttgcattaat acataagggc
     3241 tagtttcagc aaatgcggtg gttgaaaata atgcagaaac tgccactaag cctgttacga
     3301 taattttttt aatgtttttt aacattattg atgctctcca aaactctaaa ttagaggttg
     3361 ttgtacttaa ttactctgct tttggttcag cagaatcact tgctttttca tcttcagttt
     3421 tatctgactt tttatcacca tataagaact gaccgattaa atcttctaac accattgcag
     3481 aattggtatc tacaaaagta tcaccttctt tcatcattgc agtttcacct tcgattacga
     3541 agccaacatt taacgcaacg tattgctcgc ctaataagcc tgaggtttta attgagagcg
     3601 agctggtatc gggaatttga ttaaaacttt catttaccgc taatgccact ttcggggtgt
     3661 aggttttgtt atcaagtgaa atatcagcca ctcggccaac taccacaccg ccaactttaa
     3721 taggcgcacg aacctttagt ccaccaatat tatcaaaagt tgcatacagg gtgtaagttt
     3781 tctcatttga gaagccctgc acatttgcta ctcttaagcc taaaaagaca agagctgcaa
     3841 gcccaagtag tacaaataag cctacccaaa attcatattt aattgattga cgcattgtct
     3901 gttcctttca aaaaatcaac cgccgaacat tatcgcagtt aaaataaaat ccaatcctaa
     3961 aatcaccagt gaagcattta ccaccgttct tgtggtcgct tggctgattc cttctgaagt
     4021 cggtacgcaa tcgtagccgt taaataaggc aatccagacc accgcaaagg caaaaatcac
     4081 acttttaata aagccgttaa ttaaatcgct ggtatttacc gagttttgca ttaccgacca
     4141 aaaactgcca ccatcaacgc ctttccaatc taccccgact aatgagccgc cccaaattcc
     4201 gatagcggta aaaatagcag ctaacaccgg cattgaaatc acgcctgccc aaaagcgagg
     4261 agcgatgata cgtctaagcg gatcaaccgc catcatttca agactggaaa gttgctctgt
     4321 ggctttcatc agcccgattt ctgcggtcaa tgccgaaccg gctcgccctg caaataataa
     4381 tgcagtaact acaggaccaa gttctcgtaa aagcgagagt gaaaccaaag ttcccaagct
     4441 actttcggcg gcaaaatcga ccaataccac atagccttgt aagcctaaca ccattccgat
     4501 aaataagccc gacagcataa taattaagag cgattgcact cctaacacat aaagctgttt
     4561 aatcagtaac ggggtatgtt ttctaaattc aggtttaccg attaatgctc cccataacat
     4621 aaaagcggaa cgccctaatg tacggataaa attaatcacc accgccccga tagagctaat
     4681 aaaattaacc attaaatagc tcctccgtat aatcttttgc tggataatgg aaccgcaccg
     4741 gaccatcagc tttaccggct aagaattgta ccacttgcgg atcttcgctt gcctgtaaaa
     4801 tttccggtgt accttcagca atcactcgtt tatctgccac aatataagca taatcggcaa
     4861 tacttaatac ttctttcaca tcgtgagaca ccacaatgga ggtcaaattc aatgcctgat
     4921 ttaactcttt aatcagctcg acaatcacac ccatactaat cggatcttgc ccggtaaacg
     4981 gctcatcata cataatcaga tccggatcta acgcaatcgc tcgagcaagg gcggcacgtc
     5041 ttgccatacc gccggaaagt tcggacggca ttaaatttgc cgcacctcgc aacccaaccg
     5101 cctcgagctt caataacacc aatttgcgaa ttagctcttc gggtaaacgg gtatgctccc
     5161 gaatcgggaa agccacatta tcgaaggtag aaatatcggt aaataacgcc cccgattgaa
     5221 acaacatccc cattcgttgg cgaatttcat acaactcttt gttattggct ttgcaaatat
     5281 ctctgccatc aaataaaatt tcgcccgatt caggtaaaat ctgcccaccg attagcctca
     5341 acagggtggt tttaccaatc ccagaaggcc ccataatggc agtcacttta cctttctgca
     5401 cttgcagatt caggttatcg taaatcactc gctcccctcg cttgaaggtc aagtttttta
     5461 cttcaaccaa attttccgtt tttgtcatca aataggactc aaattaataa aagtgatatt
     5521 tatggtctat caaatttgcc agaatgtcaa aaaactttta ataatctgac aaaaatctgc
     5581 ctttacaagc ggtcagattt gctaaatttt ttgcaaaccg gctgaactga aaaagaaagg
     5641 tcttgtctta gccaatacga gcgttttcac tcgtagatta taaaaacata ggatatgagg
     5701 aacattcaca atatgaacaa aacaatgaaa aaatcatttt taactgcatt cattttaggc
     5761 tcaaccattg ccgcaatgcc ggcgtatgca gaagcaacat tacccacagc cgtaaatggg
     5821 caagctgtgc caagccttgc accaatgctg gaaaaagttc gcccggcggt ggtgagtatt
     5881 gcagtagaag gtaaaaccaa aggcgatagc cgccgcagtc gtgaaattcc ggaagaattt
     5941 gaatttttct tcggtcctca cgccgattta ttcggcaacc gctcatcagc tccacgcaat
     6001 ttccgtggcg ttggatcagg tgcagtcatc aatgccgaaa aaggttatgt gattaccaat
     6061 aaccacgtga tcgacaatgc cgataaaatt accgtaaaac ttgaagacgg acgtgaattt
     6121 aaagctaaat tagttggtgc tgatcccctt tcagatgtgg cgttaattca agttgaagat
     6181 ccaaaaaact taactgaaat taaattcgcc gattccgata aattacaagt gggcgatttt
     6241 acagttgcaa tcggtaaccc gtttggctta ggacaaactg ttacttcagg catcatctcg
     6301 gcacttggtc gttcaaccgg tcaatccgat gaaggctatg aaaactatat tcaaaccgat
     6361 gcggcagtta accaaggtaa ctctggcggc ccgttaatta acttaaacgg cgaattaatc
     6421 ggaattaaca ccgctattat ttctccaagt ggtggcaatg ccggtatcgc ctttgcgatt
     6481 ccaagtaata tggcaaacag cttggtgact caaattattg agtttggtga agtaaaacgt
     6541 ggaatgttag gcattaaagg cggtgaattg aatgctgacc ttgctaaaga gttcaatatt
     6601 tccacccaac aaggggcatt tgtaagcgaa gtcttaccgg attctgctgc tgctaaagcc
     6661 ggctttaaag caggtgatgt gattactgaa atcaacggtc aaaaaatccg tagtttcagc
     6721 gaattacgag caaaagtagc tacttcaggt gtaggtaaag agattgaact tacttacctg
     6781 cgtgacggca aagaagccaa agcaaaagtg accttacaat cggattctga agccaaagtt
     6841 actgcttcaa acctcattcc ggcattaaaa ggggcagatt ttaacaacta caatgccaaa
     6901 ggcattaaag gggttgaaat ttctaacgta gaaaaaggct cgattgctga aatgcgaagc
     6961 ctcaaaaaag gcgatattat catcggcgtg aaccgccact cggtagaaaa cattgccgat
     7021 ttacgcaaaa tattagaaac caagccatct gtggttgcac tcaatattgt gcgtgacggt
     7081 gcaaatttct atgtgatttt gcaataaacc ttttgatcca ctcaaacaaa agccgatgac
     7141 aaaaatcatc ggcttttgtt attgctggga atttaacatc tgtcgtgcca tttccatact
     7201 ttggataatc gctctgcggt tgatagaatc attattgcta tctaacactt tacgccaatt
     7261 atcgagagct tgttgaaaat tgttatttaa aaagctgtcg gaggctagca ggagtaaact
     7321 ggcattttca tttttatcca actgtagggc ttgctcgata atggcttgaa tttcaacggt
     7381 cattttctgc ccgttatcgt aataacgagc ggtggcaatc gcccccaaaa tagccgctcg
     7441 ttttccgagt agcttctcgg cattatcgta gcaaaccagt gccgaatcaa aatcattgct
     7501 taacgcataa gcctgcccta gttcgtacca taaatcaccg ttattcgggt tttctcgaag
     7561 ctggttttgc agactcaaaa tatagcgttc atttttcgat gtgccgtgtg catcgctcat
     7621 ctgttgttga aaatcttgat gttgttgcaa acctgattgc accacttgat aacgtccgga
     7681 aagcccatag taagtcattg ctaacaacaa aatcgccacg caactcaggc ttgcgatttt
     7741 agcaagcggt cgattttgag cattattttg caaaacgctc tgttcaaatt gcgttcgttc
     7801 aaattggtaa cgttgctctg cctcacgctc aaactcttgt ctgacgtcat ctgcaaaatg
     7861 ttgggcaaat ttaaccgctt gttggtagtt ttcttggctt aattgctcac gcgttttcat
     7921 ttttgttgcc tttatttttg cgagttcgcc acaaaatagc tagaaataac aggctaaaca
     7981 agccaaccgg caagccccat aatacccatg tctgtggcgt gaatggtgga tcgtaattca
     8041 caaaactgcc gaaacgctcg gtcataatgc ggataatttc ctcattactt ttcccttgat
     8101 tcaccatttc atacacttca attcgcatat tgtaagccgc cgtcgcattg gattcgacca
     8161 aattttggtt ctgacattgc aaacatcgca acgaacgagc cagtgaaatc gcccgtaccc
     8221 gatccgcctc attttgaaat tgaaaggtgt ccaccatttc agcttgaacc gtgccgacaa
     8281 atagcaacaa gccaaataca agcggtaata ttttgcggat tttttgcaaa aagccgatgg
     8341 aaattatgct atttctcatt tctgagagcc tctaatttgg gtaggaaatc ctgtgcaaaa
     8401 tcagggttgt aacccttttg ttggtaacgc actacaccgt gtttatcaat caaataactg
     8461 gaaggtgcag aagagacttt cagggtttgg atcaaaaagc ctttttcgta atcatcaata
     8521 attaaactaa acggatttcc ccagtttttc aatgcgttga gggcatcatt tgggcggtcg
     8581 ctataagtta agccgacaat cggcacgcct ttttgtttta gttccaacaa aatcgggaac
     8641 tctttaatgc accaagtaca ccagctcacc cacacattga gaatataagg ctctttcggc
     8701 aagctgttgt tatttaaatg cgcgttacta tcaagtaagt tcttgcctac aaactctgga
     8761 aacggcttgc cctgccaatc ttcagtggga gcaagggcat ctttattcat taacggcaca
     8821 gtcaaaaagc caacaataac tacaagtaac aggagcggaa taagtaacag gatttttttc
     8881 attttttgtt ttctctacga taaccgaata atgccaacag ggcggccatt accatcatca
     8941 taccacctaa ccataaggct tgcacataag gcttatagtg caaacggaag gcatattcta
     9001 aattgccgaa cttatccccc atcacaatat aaatatcgtc taaattgtgg tggaaaagcc
     9061 ctacttctgc cattgtcatc gttcttacat cataatagcg tctttccggt gtgacggttg
     9121 ccagcaactg ttcatctcgg ctgatagaaa aaacggcttg ctcggcggtg tagttcggac
     9181 cgatgagatc gtgataatct tcatatttaa aggtataacc ggcaagttcc gctgcttgat
     9241 tcggctttaa ccgcacgccg atttcatcgc cgtaataact gttcatcatt gccccaatca
     9301 cgcaaaccgc aaagccgata tgggctaacc gcatcgcaag cggtcgaatt tgccacattt
     9361 tttgcgaata gggcaaatga gtgagaataa tccagatcgc aaggctgata aatatggtgg
     9421 gtaacggctg caaagcaaaa tgctctcttt gcgagatgga ggcataaatc aaccctaacg
     9481 ctaaaccgac tgcaatcggc agcaaccaaa gttttaccaa tatctgctta tttgaaatct
     9541 gtttccagcc cagcaccacc gcaaagccca ttactatcat taacagtaac gctagtggcg
     9601 tgaatacgct attgaaatag ggggccccca ctgaaatcga tccccaattc atcactgtga
     9661 agatcatcgg gtagaaagtc cccagcaata ccacagaagc agccacacta aataagccgt
     9721 taatcaataa aaatgccgtt tctttagaaa gtaagttgaa acgcactttg ccttgccaga
     9781 gattcgctcg gaatgcaaac agagcaaggg caatgaaact taagccgaag aataagatca
     9841 gcagtgccat tcctctgtcg ggatcgaccg caaaggcatg aaccgaggtc agtacacccg
     9901 aacgaacaat aaacgtgcct aataggctta aagcaaaggc gaaaatggca agtagaatcg
     9961 tccaataata gaaaatgcca cgctgttggc tcacaattaa actatgcacc aatgcggtgc
    10021 caagtagcca aggcattagg gaggcatttt ccaccggatc ccaaaaccac cagccacccc
    10081 agccgagttc gtaatatgcc caccacgccc ctaaaataat gccggcggtg agaaatcccc
    10141 acgaaatcat cgtccacgga cgaagccaac gcataatcgc agcgtccatt ccgcctgaaa
    10201 cgagggcggc gacaatcatt gcaaagctca ccgcaaagcc gacatagccc aagtagagca
    10261 aaggcgggtg aaagatgagc ccaatatctt gcaacattgg gttgagatct cgcccttcag
    10321 gtggtggtgg aaagccacgt tcaaatgggc tggaaaccaa tagaatgaaa agtagaaaac
    10381 ctagtgcaat tagccctaac atcgctaagg ttcggttggc aaacaagcgg tcaatttttt
    10441 gcgaaaattt gcaaaatacc gcagaccaaa tcacaagagc ggtgagccaa aacagcattg
    10501 agccttcgtg tccgccccaa gtggcggcaa atttaaaaaa atcggggagc tggctgttgg
    10561 aatgagcggc aacgtagata accgaaaaat catcggttag aaaagcataa gctaaggtag
    10621 aaaaggaaaa tgtggtaaaa gcagcttgca aataagtgaa aagcggagcc aatgccagcc
    10681 aatgatattg cttgcgaact tcgcctagaa gtgcaaaacc aacttgaaaa atggcactaa
    10741 ttgcagccaa taaaagtgaa aaatagccga gttccggtag cataaaaaat ccaaaaaatg
    10801 accgcttgtg aatgtaaaaa aagccctctc cacttgtggg agagggacag atttttcttc
    10861 gttcagaaga aaaatcaggg agaggggaat taaataattt tgctaaaaat aacaatactc
    10921 cctctctctg aaaaatattt ctgaacgaaa tatttttcgg tctcaatact cgctacgctc
    10981 gctccttcgg agccgttggc aaagccaacg ttcaaatgta aacatttgtc ccacaagggg
    11041 agagagtata tcctaacttg caaccgttag ttgccctgca taaaggataa agtaacgcaa
    11101 gcagagtaca ccgattaagt cgaaaacgga aattaagata ataaatttca cgttatgttt
    11161 caagcggtgg ctaccaaaaa tattggcaac aagcggaatc gcaataccga ttaacattac
    11221 gcctatccag aacacattcc cccagaagcc gttaagtgca tgggttaaag cgagcgattt
    11281 atctgctcca ccgaagtgta agcccacaaa gaacgctaca attaagccta attcggttac
    11341 cataatcggc acttcaaatt tgtgcataaa gtggacttcg tggctttcac cggataactt
    11401 gcctgcgagt aaaatcacta ggaaagttgc tgcaatacct gacgatgccc ctgatgccaa
    11461 gaacaatgcc ggtaacacag ggttattcag cattggataa ctcactaacg ccgagagtaa
    11521 gaaaccggtg taagcaccaa gtacagcagc caatacgaat aagataaatt caattggttt
    11581 taacagcttc gcttcggcaa aatcgatcaa accagagacc acaggtagat accgaacaat
    11641 ccaatcttta aacattatgg cgatccaaac caccaatact gccatataaa gttggaataa
    11701 cattaccccc atcgacatta ctgaggtaaa gttatagttg aacattaatt tccagaatgt
    11761 ccaaggtttg gttaagtgga aaatcagtaa cagtaagccg ataatggttg gtaatgtgcc
    11821 aagaatagcc gcactacgaa taatccagtt ttggcttggt ttttccattt tgacaccgct
    11881 attacggtaa gcaatcgcta actgaaccgc acctgatgaa ataccaagta ggaataagta
    11941 aatagcgata gtgccatccc aaacaaggtt tggggtttgg aaaggtacat attcattcat
    12001 tatctatgtt ctcctttacc aaatggaatg tgatataaat tcggctccgt gcctaatgcc
    12061 gttttggtgc gataaaccgg attatttttc actttatgga aaatatcgct acttggatca
    12121 ttcatatcac caaaggttaa agctttagtc ggacaagcct ctacacaagc aggctgtttg
    12181 ccttctgcaa gattggtatc tcggcaaaag ttacatttat ccgccgattt tttctcaggg
    12241 tgaataaaac gtacacgata cgggcaaacc gcaatacagt attggcaacc gacacacaaa
    12301 tctgagtgta catcaataat cccggtttca ggatcgataa atgaagcacc ggtcgggcaa
    12361 acactcacac aaggtgcatt ggtacagtgt tggcaagatt ggcggaaaaa ctcatattgt
    12421 acgttcggaa actcaccgaa aggctcacta cggataattt ctaaacgaga aaccccttcc
    12481 ggcactttgt tggtggtgcg acaagcgtcc atacaagccg tacagccgat acaggcagtt
    12541 tcatcatgaa ccatcgcata gcggattttc ttttgttctt tctcagtagc aagagttatg
    12601 gaggtggcaa gccctgtact tgccacaact gcgatagcac ctgcccctga gacgaaatct
    12661 ctacgggtgc aagtcattat tttgttcctt ttgcttctgt ggctttcatt tcaggatttt
    12721 taaattcgcc tgaatgaatt ttggtatgac aatccgtaca aagtttaatg cgatcttttt
    12781 caggaatgtc tttcatcgga tctttttccg ggtgaatttc atgacagttg gtacaagcaa
    12841 ttttagttgc gtgcgtgtcg tgagcccaga atttttcacg caatttctct gccgtatggc
    12901 aagcaaaaca gacttgattt tgctcattta ccgaacgttc aagtgatggg ttacttgctt
    12961 taccgtgtgg gttaaaacgc attacgtctt ttgccccttt gcggtggctt tcagaaatat
    13021 taccgtggca acttatacaa gtgagtggct ctccattatt cgggcttttc acatcttttt
    13081 ggaaatgttt gcctgcgtga tggaaaggct tacctgttgc gtttgccgat tcgtgacatt
    13141 gagtacaata tttatttgga tcacggcgtg catcttgagc tacttttgca tcatcagccg
    13201 tttctgattg ccaaagaggt tttggctgct caggcattaa cgcattcgca ctggaaattg
    13261 caaaaaaacc tgcaaatacg accgcttgta tgagggcgac tgctcgtcct gcttttgaga
    13321 ttaatttttt catagttcac ctcaagctac attattttgc ttctgctttt ggtgctgcta
    13381 ccggtttgtc tgcttcttct gcgggtaata aacctttagc acgagcttct ttttcccatt
    13441 gaggaaccac agtttttaag aactcatctt ttgctgcttg ctctttagga atatctaaac
    13501 caatagcaag ttgtgctttc tctttagttg agatgtccgg aatcgccact ggcgttgtta
    13561 caccgtgttt cgctaacact acacctaatt tagcacgggc atctgctgca cggtcgatac
    13621 ctgttgcgat ggtacgtaac attacatcag gtgcgtggaa gtggttaccg tgacctgccg
    13681 cagagtaatc ccaacgccat tgagcgtgac gaattgccat taagatctct ttcatctctt
    13741 cttgagttgc tccagcttcc caagcagctt tcgcttcaaa gtgagctttc actaattgat
    13801 cttctaagcg aatcattgcc tcttttactt cgtgtttata ctcttttaca cgtttttcca
    13861 atgtttcttt gctttgttca tgacaagttt tacaagtcgc ttcaaattgg tcaaacggat
    13921 taccgatttt gtggtcagta taaactttgc cgtctttgtc tttcactttt ggcatatggc
    13981 aatcaataca ggttacacca tttttcccat gtgtaccttg tgaccaaact tcaaaatctg
    14041 ggtgctgcgt ttttagcatc ggagctttcg ataaagcatg agtccagtct gtaaaattga
    14101 tttcatcgta gtatttttca atgctatcta catctacacc tttatcccaa gggaaagtta
    14161 catctgtttt atttttgaaa tagtactcca catggcagtt agcgcacaca gcagggcgtt
    14221 taccatgttt atctaaatcc tcaaacttcc aaccaaccgt attcaatgca cgtaatacgt
    14281 gaggacgtgc aacacgtagt gcaggtttac cttgtttaaa ctcttctgat gtcgtgtcat
    14341 ggcagtctgc acaacctatt gagtttacaa tttcagcacc atatttcgcc catttaggat
    14401 caaaatatcc tcgctcacct ttctcctcaa ttaagcgtgg aacatcaggg cctttacatg
    14461 tccaacaagc cataggttgt ggaccatcat tttcatcttt aggcgcgccg gtgcgcaaaa
    14521 tatcacgcac atcggtaact gcataaaagt gacctcgtgg tttattataa tctttcgcaa
    14581 aagcatagcc tgcccacaaa ataacataac gagggtcagc ctctaacgca cttcctcgat
    14641 ctgtggattt tgcagttgac ttccaagaat cgtattgtag cggatatttt tctgcaaata
    14701 ggtggtttgc agactcaatt ttcacgccta atttttcatg cttaactgct ttatctgtta
    14761 atgattcagt tttcgcctct tcagctaaag cagtttgtaa acaccccaat attgaaattg
    14821 ttgccacagc taggcttttt attaaaaatt tca
//
LOCUS       DANLZC010000056        14564 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.056, whole genome shotgun sequence.
ACCESSION   DANLZC010000056 DANLZC010000000
VERSION     DANLZC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14564
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.056"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..675
                     /gene="purU"
                     /locus_tag="PXE44_001288"
     CDS             <1..675
                     /gene="purU"
                     /locus_tag="PXE44_001288"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDL5588801.1"
                     /translation="IFNDETLLTDLKFTLPEGSSYKLLPKQRKRIVILVTKEAHCLGD
                     LLMKNYYGGLDVEIAAVIGNHETLKSLVERFDIPFHLVSHENLTRVEHDKLLAEKIDE
                     YSPDYIVLAKYMRVLNPEFVARYPNRVVNIHHSFLPAFIGAKPYHRAYERGVKIIGAT
                     AHFVNDELDEGPIIMQNVINVDHTYTAEAMMRAGRDVEKTVLSQALELVLADKVFVYK
                     NKTIIL"
     gene            complement(712..1395)
                     /locus_tag="PXE44_001289"
     CDS             complement(712..1395)
                     /locus_tag="PXE44_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5588802.1"
                     /translation="MNQTRDRLEKLPLFHKMQQFIIKALKIIAIFPLLGIAILLVVDS
                     LTSYIVRDKIYTDSKQIPYREYAVVLGTAKFYSKDVINQYYKYRLEAAKQLVQEGKVN
                     QLLLSGDNKTPYYNEPKTMTSDLLKMGIATSKIKQDYAGYTTFYSIIRAKKVYKLPPF
                     TIISQKFHCERALFIAKFRDIDAICYAATYPEGAYQVRMREILARSAMVFSLLIGKEP
                     ESLEDIRSK"
     gene            1663..2736
                     /locus_tag="PXE44_001290"
     CDS             1663..2736
                     /locus_tag="PXE44_001290"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HDL5588803.1"
                     /translation="MAQQITLFEDVPLPLTLGGELSPITVAYQTYGRLNAEKSNAVLL
                     CHALTGDAKPYYDSPTEKGWWQDFIGKGLAFDTDKYFFICSNVLGGCKGTTGPASINP
                     KTNKPYGSLFPIITVQDIVNVQKALLDYLEIPQLHAVVGGSFGGMQATQWGISYPDKV
                     RNIINLCSSLTLSAEAIGFNHVMRQAIINDPHFNGGNYYDGIAPDNGLKIARMLGMLT
                     YRTDIQLAKAFGRETKQQGQIWGDHFQVESYLSYQGTKFLGRFDANTYLRLLRALDLY
                     DPALGYENEETALKRIKANYTFVAVTSDQLFKQVDVYKSKQKLEQAGVNLEFHEFHSD
                     FGHDAFLVDYDFFEPMIKAGLAK"
     gene            complement(2863..3918)
                     /locus_tag="PXE44_001291"
     CDS             complement(2863..3918)
                     /locus_tag="PXE44_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5588804.1"
                     /translation="MGRPRKQQNRYLPENLRPKTVTRKSGKTVIYWLYRTHGKTEISL
                     GTNRNEAFIKAAQLNIERESQTARITFGLVAKRYRDEVIPQKKASTAQTDLCKIKNLL
                     LFFNDAPLEEITPQHIREYMIWRKDTPGSANNEFSMFSHIWQYAREWGYTNLPSPNIG
                     MKRYPIRKREIYVENNIFQMVYEVADQDMRDLMDLAYITGQRPVDLVNIRKEHIFDGY
                     LHIVQQKTQAKLRIEIVGKLADIIQDRLEKATGTHLFSTKKGEKLTARKLGKRFTYIR
                     EQAVKNNPEYANEIMAFQFRDLRAKSGTDKALSLGEEAARQQLGHTSVQMTKTYIRKA
                     PIVTPIKESVPSSKHQS"
     gene            complement(3878..4153)
                     /locus_tag="PXE44_001292"
     CDS             complement(3878..4153)
                     /locus_tag="PXE44_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL5588805.1"
                     /translation="MEQPTEFLTKDELTDLTGYRQKKRQIEWLRERHYIIQDENKYKP
                     LVLYKDVYGHPRQQYPEAQSTGKWKSPAYLMGVKNGKTTQTTESLLT"
     gene            complement(4179..4670)
                     /locus_tag="PXE44_001293"
     CDS             complement(4179..4670)
                     /locus_tag="PXE44_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5588806.1"
                     /translation="MQTKPILDACCGSRMFHFDKQNPHVLFADNRQIETTMKDSGKVR
                     HLEIKPDVLHDFTNMPYENESFWHVVFDPPHLVQGGENSWLVKKYGKLPCNWREVLKK
                     GFDECMRVLKPNGTLIFKWNETQIPVSEILKVIDCQPIYGHKSGRLNQTHWLAFVKIE
                     QSL"
     gene            complement(4733..5575)
                     /locus_tag="PXE44_001294"
     CDS             complement(4733..5575)
                     /locus_tag="PXE44_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588807.1"
                     /translation="MAKQIFKQAPLPFVGQKRMFLAQVSQILNENITDDGQGWTIIDV
                     FGGSGLLAHTAKHIKPKAHIIYNDYDGYAERLKHIPDTNRLRKQIYDIIGKSTPKNKR
                     LDPDKKSQVINIIQSFDGYIDVNCVASWLLFSGQQINSLEDLFNKIFWNGVRQTDYPS
                     AEGYLDGIEVTHESFHKLLPRFQHKDKVLLLLDPPYLCTRQESYKQATYFDLIDFLRL
                     INLTKVPYIFFSSTKSEFVRFVDFMVSEKKDNWQTFENAKWIKVKASLNYQSTYEDNL
                     VYKF"
     gene            complement(5622..5747)
                     /locus_tag="PXE44_001295"
     CDS             complement(5622..5747)
                     /locus_tag="PXE44_001295"
                     /inference="COORDINATES: protein motif:HMM:NF021626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5588808.1"
                     /translation="MQSLRLIEIRCKYCQKLLAKAKDVQHLEIKCVRCKSINKFN"
     gene            complement(5894..6103)
                     /locus_tag="PXE44_001296"
     CDS             complement(5894..6103)
                     /locus_tag="PXE44_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588809.1"
                     /translation="MFRNELQVIDGKRYIVLECQFRREWRVAIESRGTVTNGEAIEIC
                     QYWVKYKGVKPEQLKIVEVPDILKE"
     gene            complement(6106..6399)
                     /locus_tag="PXE44_001297"
     CDS             complement(6106..6399)
                     /locus_tag="PXE44_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588810.1"
                     /translation="MSESFSLEKALWEEPRPRLQLDLPCPVIKLEVGKRYYTINIHGV
                     DCYDGDMSFTEIDEWTYESIDDVQTKSRAEQGLLFASEADAQEWIDAMRNSSR"
     gene            complement(6392..6829)
                     /locus_tag="PXE44_001298"
     CDS             complement(6392..6829)
                     /locus_tag="PXE44_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588811.1"
                     /translation="MKTTQHILDEREQQHGNYDSFAKIYGGLRKVSDSHAEKLTWPQQ
                     IAVEMMLFKIARILNNGANHQDNYQDIAGYAMLGGGLYNPNVSAEVKALPKPLTDSIY
                     PESHLNKSSVWRLDLEFETKEEATEVLEALTGKSAGVSEVNNE"
     gene            complement(7068..7745)
                     /locus_tag="PXE44_001299"
     CDS             complement(7068..7745)
                     /locus_tag="PXE44_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HDL5588812.1"
                     /translation="MSNQVQFPVFNFNSSAVRVIIDPNQEPWFCGADVCRILGYVNES
                     LTLQKHCKENGVSKRYLTDKMQRQQKAIFINEPNLYRLIIKSRKPEAEKFEAWVFEEV
                     LPQIRKTGKYALQNNQQNLPLAPPPKKYSFDFTEDELQSLIWAWFAFVRGIHTFRYIY
                     PMFQKLGSNIAPEIYGQGFEYSHTAQSAHKILERITREFDCDPMTNWRVLKHLRGFDP
                     AFKKPTF"
     gene            complement(7993..8832)
                     /locus_tag="PXE44_001300"
     CDS             complement(7993..8832)
                     /locus_tag="PXE44_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDL5588813.1"
                     /translation="MANSVMIVQGVEVKVTARDGEDYISLTDMCKAFGDSDQLIKNWL
                     QNKNTIEFLQVWEELNNPNFNLVELHQIKNNIGLNRFVMSVKKWTATGAIGLVAKAGR
                     YGSGTYAHKDIALEFGSWLSPEFKLYLIKEFQRLKQQEANESKLEWSVKRILSKANYR
                     IHTDAIKAHIIPALLNTKQHTFVYSSEADILNQALFGQTAKQWKDRNPNLKGNMRDHA
                     TVEQLTVLAALESQNALLIEQGYSQEERLAMLNRLAIQQMSSLLQTRAIEELKEKPLL
                     IEE"
     gene            9062..9439
                     /locus_tag="PXE44_001301"
     CDS             9062..9439
                     /locus_tag="PXE44_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588814.1"
                     /translation="MFSLLRDALTFAAMFTGILFPAIIHKYDLSLGTQIQLLSFVPAF
                     FVLCHFLIKRKYQRDFDRLSYLSAVVCVAMCAGVGAFSQSEMIELGFKVERVLDYVTI
                     KFLLYFWSLLLLPVVLRKLFGKD"
     gene            9571..9759
                     /locus_tag="PXE44_001302"
     CDS             9571..9759
                     /locus_tag="PXE44_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588815.1"
                     /translation="MKELFKQWLINQDSPFINSCGVECILSKVDDWLNIINANEEETE
                     TLVEWLNAFLQEVSVFVA"
     gene            complement(9763..10260)
                     /locus_tag="PXE44_001303"
     CDS             complement(9763..10260)
                     /locus_tag="PXE44_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5588816.1"
                     /translation="MQQLIKNIEQWAEDRNLINGSTPQKQMLKLMEEFGELCAGVATN
                     EFETILDSLGDCLVICTIMRKQLKMEKLFSISHCDNTNFNCGVAAQIVQANIDLGNVA
                     SELSRGCDLSRLEDYLSGFENQLDLIAQFYKTDVQTALLYAYIQIKNRKGKMIDGVFV
                     KAEDL"
     gene            complement(10244..10444)
                     /locus_tag="PXE44_001304"
     CDS             complement(10244..10444)
                     /locus_tag="PXE44_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588817.1"
                     /translation="MTDTKRLDFIKKHKMEIRYNGLSDEFVISNHAENSQHFRMARNA
                     DLRTAIDEAMKEMRGLNNATTN"
     gene            complement(10441..10959)
                     /locus_tag="PXE44_001305"
     CDS             complement(10441..10959)
                     /locus_tag="PXE44_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL5588818.1"
                     /translation="MNEDKFFSVDVSNDIHILNLHETLEQAKQSCLNGATEAYEFAGD
                     MDDHESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERESELFGEVEQVIEPPTLVENNG
                     WISIEDKLPPIETDVLGLCDISGMQLILIVSRELADNEWYFLSVNQYGLDDDVIAVTH
                     WQPLPKLPKDNQ"
     gene            complement(11069..11872)
                     /locus_tag="PXE44_001306"
     CDS             complement(11069..11872)
                     /locus_tag="PXE44_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HDL5588819.1"
                     /translation="MDKSTIQQISTLAVAATKEVQTDFGTVMLPEGIKLQNLEPFQAH
                     RNQFRAAFSTQRFGSLIEYATANAQENAQCFIDQEKMSAEIVFDMGNREQAGHAKHRA
                     NLVMKKTAAYKALCEINGSRRSQRDFSDFLEDWGGYLTAYHHEDEISIKNAVQAVRKM
                     TIDYARNEEHELSDFAAKKSAMESVEAKSTLQLPTHLVFTCNPYNGLDTRSFTLRVQV
                     LTGSGEPVLTARLVQAEQIEEAIATEFAEKLSDALSETSIKVNIGTIEI"
     gene            complement(11942..12301)
                     /locus_tag="PXE44_001307"
     CDS             complement(11942..12301)
                     /locus_tag="PXE44_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588820.1"
                     /translation="MAKTNVPEFLDELDCGIFKDKLATALSEVALGVLTHDKKGKVTV
                     EFSLDKMDSDSPSVQIQHKLSYVKPTKRGKSAEEDTTATPMYVHKGGALSATPEKTET
                     AVKESPSGLKALSKAAA"
     gene            complement(12337..12795)
                     /gene="ssb"
                     /locus_tag="PXE44_001308"
     CDS             complement(12337..12795)
                     /gene="ssb"
                     /locus_tag="PXE44_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5588821.1"
                     /translation="MAGINKVIIVGRLGNDPELRTMPNGDTTAKISVATSTEWTDKVS
                     GDKKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIA
                     DQLQMLGSSSSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIPF"
     gene            complement(12798..12995)
                     /locus_tag="PXE44_001309"
     CDS             complement(12798..12995)
                     /locus_tag="PXE44_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588822.1"
                     /translation="MARKIDAGVEIVEVDKLFIAKFFYDGKLQHTTYPQYSRQNAIML
                     INRKIERFNVGRDKPIKLYKE"
     gene            complement(12979..13164)
                     /locus_tag="PXE44_001310"
     CDS             complement(12979..13164)
                     /locus_tag="PXE44_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588823.1"
                     /translation="MNKMKQLAPFALLILLLGIVGRMDYDDHIQMQRYKCERNQGVWQ
                     VESNGNQYCGGKYGTKN"
     gene            13378..13653
                     /locus_tag="PXE44_001311"
     CDS             13378..13653
                     /locus_tag="PXE44_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588824.1"
                     /translation="MKNNILITYDLNKSGQNYAALIEKIKTLGAWAKVQQSAWYLHTS
                     YSSKEVLEMLSTVTDYNDSLFVAQLSEANWRGLSNEVSKFIQEQWWK"
     gene            complement(13656..13907)
                     /locus_tag="PXE44_001312"
     CDS             complement(13656..13907)
                     /locus_tag="PXE44_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588825.1"
                     /translation="MKNKNFEIAKVVILNEQLGSPKDLELKVVENGLSDEDKYHIKQA
                     VLKAVENGCLDPEMLASRCCSAIETINKSGINTGNGSCE"
     gene            complement(14360..>14564)
                     /locus_tag="PXE44_001313"
     CDS             complement(14360..>14564)
                     /locus_tag="PXE44_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588826.1"
                     /translation="HKDYILCGRKEKRTSDFINVFEVFENETTQEFVIERAMFRNGKL
                     IDWNQSDKMNAEQAQQLWQAYIH"
BASE COUNT         3970 a         2912 c         2766 g         4916 t
ORIGIN      
        1 atttttaatg atgaaacctt actcactgat ttaaaattta ccttacctga aggctcaagc
       61 tataaattac tccctaaaca aagaaaacgt atcgttattt tagttaccaa agaagcacat
      121 tgtttaggtg acttactgat gaaaaattac tatggtggat tagatgtaga aattgctgcc
      181 gtgattggaa accacgaaac attaaaaagt ttagttgaac gtttcgatat tccattccat
      241 ttagtcagcc acgaaaattt aacccgagtg gaacacgata aattattagc ggaaaaaatt
      301 gatgaatatt cacctgatta tattgtgttg gctaaatata tgcgggtgct gaatccggaa
      361 tttgtcgctc gttatccaaa ccgagttgtc aatattcacc actcgttctt acccgctttc
      421 attggtgcaa aaccttatca tcgtgcatac gaacgtggag tgaaaattat cggtgcaacc
      481 gcccattttg tgaatgatga attagatgaa ggcccgatta ttatgcaaaa tgtgattaat
      541 gttgatcaca cctacaccgc agaggctatg atgcgtgccg gacgtgatgt cgagaaaacc
      601 gtacttagcc aagccttaga attagtgctt gctgacaaag tatttgtata taaaaacaaa
      661 accattattt tataagcaaa atagccggtc aacattatct gaccggctat ctcatttaga
      721 acggatatct tccaatgatt caggctcttt acctatcaac aaactaaaaa ccattgccga
      781 tctggctaaa atttcccgca ttcgtacctg ataagccccc tctggatatg tagcggcata
      841 acaaattgca tcaatatccc taaattttgc aataaacaat gccctttcac agtggaattt
      901 ttgggagata atcgtaaatg gcggtaattt atatactttt ttagccctaa ttattgaata
      961 aaaagtggta taccccgcat aatcctgctt aatttttgaa gtcgcaatcc ccattttaag
     1021 tagatcactt gtcattgttt ttggctcatt atagtacggc gttttattat ctccacttaa
     1081 caaaagctga ttgacctttc cttcttggac taattgctta gcggcttcca aacgatattt
     1141 ataatattgg ttaatcacat ctttagagta aaattttgcc gtaccgagta caactgcata
     1201 ctcccggtaa ggaatctgtt tactatctgt ataaatttta tccctcacaa tataacttgt
     1261 aagtgaatca accactaata atatagctat tcccaataag gggaaaattg caattatttt
     1321 caatgcctta ataataaact gctgcatttt atggaaaagt ggcaattttt ctaacctatc
     1381 acgcgtttgg ttcaaatgag atcctttact tttgctaccc tatttaagat gggtaaatta
     1441 gagacaaaca aaatagtcta atcgttcaat aaatttattc tatttcatgt tatttgttaa
     1501 gtaataaata tcttctcagc aataaaattt tgattttagc cgtttaaaaa tctagacgtt
     1561 tagacgtcta aaaattcttg acaattgaaa tgaacaccgc taatttaacg gtcattacaa
     1621 caagcggtca aaaaagcaga attttttgca aacaggattt ttatggctca gcagattacc
     1681 ctttttgagg acgtaccttt accactcaca ttaggaggcg aactgtcccc tatcacagtg
     1741 gcttaccaga cttacggcag attaaatgca gagaaaagca atgcggtctt gctttgccac
     1801 gcccttaccg gtgatgctaa gccttactat gactcaccaa ccgagaaagg ctggtggcaa
     1861 gatttcatcg gcaaagggct tgcgtttgat accgataaat atttctttat ttgcagtaat
     1921 gtattaggcg gttgtaaagg cacaaccggc ccagcttcga ttaaccctaa aaccaataaa
     1981 ccttacggta gtttgtttcc aattattaca gtgcaagata ttgttaatgt acaaaaagca
     2041 ttattggact atttagaaat ccctcaactt cacgcagtgg tagggggatc tttcggcgga
     2101 atgcaagcaa cacagtgggg tattagttac cctgataaag ttcgcaacat tataaatctt
     2161 tgttcgtcac ttaccctaag tgccgaggct atcggtttta accacgttat gcgccaagct
     2221 attattaacg atccacactt taacggtggg aactattatg atggcattgc acctgataac
     2281 ggcttaaaaa tcgcccgtat gctcggtatg ctgacttacc gtactgatat tcaacttgct
     2341 aaagcctttg gacgagaaac caaacagcaa ggacaaattt ggggcgacca tttccaagtg
     2401 gaatcctatt taagctatca aggcacgaaa tttttaggtc gtttcgatgc caacacttac
     2461 ctccgtttac tccgagcatt agatttatac gacccggctc ttggctatga aaatgaagaa
     2521 accgccctaa aacgtattaa agccaactac acttttgtag cggtaacaag cgaccaacta
     2581 ttcaagcaag ttgacgtgta taagagcaaa caaaaattag aacaggcagg cgtaaatttg
     2641 gagttccacg agtttcactc cgatttcgga cacgatgcct ttttggttga ttatgacttt
     2701 tttgaaccga tgattaaagc cggattagca aaataaattg cattttttat taaaaatatt
     2761 atcagtaata acgagtaaca aaaattactt atctttcaat ctattataag tgatttttat
     2821 gtaaacgttc cgaaaaaatg caaaatgttc cgaaactgtc ttttaagact ggtgttttga
     2881 ggagggaaca ctttctttga taggggttac tattggggct tttcgaatgt atgttttggt
     2941 catttgaacg ctagtatgac ctagttgttg gcgagcggct tcttcaccta gagaaagggc
     3001 tttatctgtt cctgatttag ctcttagatc ccgaaattgg aatgccatta tttcatttgc
     3061 gtattccggg ttattcttga ccgcttgttc ccgaatataa gtgaaccttt tacctagttt
     3121 tctagcggtt agcttttccc ctttcttagt gctgaataag tgtgttccag tagctttctc
     3181 cagcctatct tggataatat cagccagttt tcccactatc tcaatacgta gtttggcttg
     3241 tgttttttgc tgaacaatat ggagataacc atcaaaaatg tgttctttcc ggatattgac
     3301 caaatcgaca ggacgttgtc cggtgatata ggcgagatcc attaagtctc tcatatcctg
     3361 atctgccact tcatatacca tctgaaatat attgttctca acgtaaattt cacgctttct
     3421 gatagggtag cgtttcattc cgatattagg gcttggtaag tttgtataac cccactctcg
     3481 agcgtattgc caaatatgac tgaacattga aaattcgtta ttagcagatc caggtgtgtc
     3541 ttttcgccaa atcatatact ctcggatatg ttgaggggtg atttcttcga gaggagcgtc
     3601 attaaagaat agcagtagat tttttatctt gcacaaatct gtttgtgctg tgctcgcttt
     3661 tttctgaggt ataacttcat ctcgataacg tttggctaca agcccgaagg taattctggc
     3721 agtttggctt tctctttcta tattgagttg agcagcctta ataaaagcct catttctatt
     3781 tgtgcctaga gagatttccg tttttccgtg cgtgcgatat agccaataaa tgacagtttt
     3841 gcctgatttt cgtgtaactg tttttggacg taaattctca ggtaagtagc gattctgttg
     3901 tttgcgtggt cttcccattt ttcactccca ttaaataagc aggtgatttc catttcccag
     3961 tggattgagc ttctggatat tgttggcggg ggtgcccata gacatctttg tatagcacta
     4021 atggcttata cttgttttca tcttgaatta tatagtgacg ctccctcaac cattcgattt
     4081 gccttttctt ttgacgatag cctgttaaat cagtaagttc gtctttcgtt aaaaattcag
     4141 tcggttgttc catattctct ccaaataacc ccctcaattt agagggattg ctcaattttc
     4201 acaaacgcca gccaatgtgt ttggttcaag cgtccgcttt tgtgtccata aatcggctga
     4261 caatcaataa cttttaaaat ttctgaaacg gggatttgtg tttcattcca cttgaaaata
     4321 agcgtgccgt ttggttttag cactcgcata cattcgtcaa agcctttctt caagacttct
     4381 cgccagttgc aaggtaattt gccgtacttc ttcacaagcc agctattttc tccaccttga
     4441 actaaatgtg gcggatcaaa aacaacgtgc caaaaactct cgttttcgta tggcatattc
     4501 gtgaagtcgt gtaaaacatc aggtttgatt tctaaatggc ggactttgcc actgtctttc
     4561 attgtggttt ctatttgtcg attatcagca aataaaacgt gtggattttg cttgtcaaaa
     4621 tgaaacatac gggaaccaca acaggcatct aatatcggtt ttgtttgcat tttttctcca
     4681 aaaaataacc gcttgcggaa caagcggtta attgtcaatc attcattaaa tgtcaaaatt
     4741 tataaaccaa attatcttca taggtagatt gataatttag actagcttta actttaatcc
     4801 atttcgcatt ttcaaaagtt tgccagttat cttttttctc gctaaccata aaatctacga
     4861 acctcacaaa ttcacttttt gttgaactaa aaaagatata aggcacttta gttaaattga
     4921 ttaatcttaa aaaatcgatt aaatcaaaat aagtggcttg cttatagctt tcttggcgag
     4981 tacaaagata tggcggatca agtaataaca ataccttatc tttatgctga aaacgtggta
     5041 atagtttatg aaaactttcg tgagtaactt ctattccatc taaatagcct tccgcacttg
     5101 gataatcggt ttggcgaaca ccattccaaa atattttgtt aaatagatcc tctaaactat
     5161 taatttgctg tccgctaaat agcaaccaag aagccacaca atttacatca atatacccat
     5221 caaaagactg aataatattg ataacttgag atttcttatc aggatctaat cgcttgtttt
     5281 taggcgtact cttcccaata atgtcataaa tttgcttgcg tagcctattt gtatcgggaa
     5341 tatgctttag ccgctccgca tatccatcat aatcattgta gatgatgtga gcttttggtt
     5401 tgatatgttt ggcagtatga gctaataacc cactaccacc aaatacatct atgattgtcc
     5461 aaccttgtcc gtcatcagta atattctcat ttaaaatttg agaaacctga gctagaaaca
     5521 ttcgtttctg acctacaaac ggaagtgggg cttgtttaaa gatttgcttt gccatagttc
     5581 tcctatggcg ttcaggcgtt cttaacgctc agacactcaa ttcagttaaa tttattgatt
     5641 gatttacaac gtacacattt aatttctaaa tgttgtacat ctttcgcttt tgccaataat
     5701 ttttggcaat acttacagcg gatctcaatt aatcttaatg attgcatata aatacacttt
     5761 tacttcattt tttgataaaa ttccgcctac ctagctaggt ggcggcgtat ggctatatgc
     5821 aagcgtgctt tgcgtagctg gcattttaag tgttaccgca cttataatgt cgccgttttt
     5881 aactcatttg tccttattct ttcagaatat ctggcacttc aacaattttc agttgctcag
     5941 gttttactcc tttatattta acccaatatt ggcagatttc gattgcttct ccattcgtta
     6001 cagtacctct agactctatt gcaactctcc attcgcggcg aaattggcat tcaagtacga
     6061 tataacgttt accatctatt acttggagtt catttctaaa catagctacc tactactatt
     6121 tcgcattgca tctatccatt cttgagcatc tgcttcactg gcaaatagta acccttgttc
     6181 agctctgctt tttgtctgca catcatcaat actttcataa gtccattcat caatttcggt
     6241 gaagctcata tctccatcat aacagtcaac gccatgaata ttgattgtat aatagcgttt
     6301 accaacttca agttttataa cagggcaagg taaatctaat tgcaatcttg ggcgtggttc
     6361 ttcccacagt gctttttcta aactaaatga ctcactcatt atttacctca ctaacaccgg
     6421 cacttttacc agttaaagct tccaacacct cagttgcctc ttctttggtc tcaaactcca
     6481 aatccagccg ccaaacagag cttttattaa ggtgtgattc ggggtaaata ctatcggtta
     6541 atggttttgg taatgcttta acttctgccg acacatttgg gttataaagt cctccaccta
     6601 acattgcata accggcaatg tcttgataat tgtcttgatg atttgctccg ttatttaaaa
     6661 ttcgggcaat tttgaataac atcatctcaa cggcgatttg ctgtggccaa gtgagctttt
     6721 ctgcgtgtga gtcgctgact ttgcgtaaac caccataaat cttggcaaaa ctgtcgtagt
     6781 tgccgtgttg ttgttcacgt tcatctaaaa tgtgttgagt tgttttcatc gtgaattttc
     6841 ctttagaaat agccgcttgc ggaacaagcg gttgggttaa ttagcgttga gccatacaaa
     6901 ttttgccatc gcagtcgttg cgtaactcta gatggtcagc agcaactgca aaggcggtta
     6961 aaaagagtat gatttttgcg tatttcatcg tctaatcctc aaaatttggg tgtggaaatc
     7021 cgccgcttga tgtttcgcaa gcggtcgggt tttataattt tttgtgatta gaacgttggc
     7081 tttttaaatg ccgggtcgaa accacgaagg tgtttgagta cacgccaatt cgtcattggg
     7141 tcgcaatcaa attctcgggt aatccgttca aggattttat gagccgattg tgcggtgtgg
     7201 ctatattcaa aaccctgtcc gtagatttca ggtgcgatat ttgagccgag tttttgaaac
     7261 atcgggtaga tatagcggaa agtgtgaatg ccacgcacga aagcgaacca agcccatatg
     7321 aggctttgga gttcatcttc ggtaaagtcg aaagagtatt tctttggcgg tggtgcaagc
     7381 ggtaggtttt gctgattatt ttgcaacgca tatttaccag tcttgcgaat ttgtggcaga
     7441 acttcttcaa atacccacgc ttcaaacttt tccgcttctg gtttgcgtga tttgataatc
     7501 agtcggtata ggtttggttc gttgatgaag atcgcttttt gttgtctctg cattttatcg
     7561 gtgaggtatc gtttcgatac cccattttct ttacagtgtt tttgcagagt aaggctttca
     7621 tttacataac ctaaaattcg gcaaacatct gcaccgcaaa accacggttc ttgatttgga
     7681 tcgataatga cacgaacagc agatgaattg aaattgaaaa cagggaattg aacttgattt
     7741 gacatttttt gtaccttttg gatttaagta tttaatccga tcattaagtg ggtgatcggg
     7801 gttcaactta ccctccaaaa gatgggcgga gcttattcag atgtattgta tatcgctctc
     7861 tcgccccgat catttcggat taaacctaaa atttaggtac aaaaaaaccg ctttatatcg
     7921 gaggcggaaa ccgcttttgg aaagtaagtg tggttatctt agccgaaagg gcggtttttt
     7981 gtcaattaga ttttattctt caattagcaa tggcttttct ttcagttctt caatcgctct
     8041 ggtttgtaac agcgagctca tttgctgaat agccaaacgg ttgagcattg ccagtcgctc
     8101 ttcttggctg taaccttgct caattagtag ggcgttttgg ctttccaatg cagcaaggac
     8161 ggtaagttgc tcaacggtag cgtggtctcg catattgcct ttcaggttcg gattgcggtc
     8221 tttccactgt tttgccgttt gtccgaagag agcttgattg agaatgtccg cttcgctgga
     8281 ataaacgaaa gtatgttgtt ttgtattcag caatgccggt atgatgtgag ctttgatggc
     8341 atcagtatga atgcggtagt tcgccttgct taaaatgcgt tttacgctcc attcgagctt
     8401 actttcgttc gcttcttgct gtttgagtcg ttggaactct ttgatgagat aaagcttaaa
     8461 ttctggactg agccaagaac caaattcgag agcaatatct ttgtgggcgt aagtgccgct
     8521 accatatcgt cctgcttttg ctactagccc aatagctccc gttgcagtcc attttttgac
     8581 agacatcaca aagcggttta agccgatgtt gtttttaatt tggtgtaatt ccaccaaatt
     8641 aaaatttgga ttattcagct cttcccaaac ttgaagaaat tcaatggtat ttttattttg
     8701 aagccagttt ttaatgagct ggtcactgtc tccaaaagct ttgcacatat cagttaggct
     8761 gatgtaatcc tcaccatcac gagcggttac tttgacctcc acgccttgta caatcattac
     8821 gctatttgcc atttcgtttc tccgttttta ggtacaaaaa aagccgttgg tggacggctt
     8881 gaaagtgcgg ttatcttaat ccgaattggg ggcggtgtca attagattaa tccgtataag
     8941 tatggaacca agttaaagag tagaactaca acacataacc aaaaaatgaa attcatatct
     9001 tgctcagttt tgattttgtg taaaatacgc ttaagtttgt ttttcattta ggaaaccttt
     9061 aatgtttagt ttattgcgtg atgctttaac ctttgctgca atgtttacag ggattttatt
     9121 tcctgcaatt attcataaat acgatctatc acttggtaca caaatccaat tacttagctt
     9181 tgttccagct ttctttgtgt tgtgccattt tcttattaag cgaaaatatc aacgtgattt
     9241 tgacagactt tcgtatttat ctgctgtggt ttgcgtagca atgtgtgcag gcgttggagc
     9301 atttagccaa tcagaaatga ttgagctagg ttttaaagtt gagcgtgttc ttgattatgt
     9361 gactatcaaa ttcttactct acttttggag cttgctgtta ttgccagttg tgctaagaaa
     9421 attgtttggg aaagattagt tggttattta aagagagcta tttgtcaaac ctcaatatct
     9481 atttttgcga tctgcatcga aaaaataaat tttcgttctg acagtaaaac gctttcgtag
     9541 taatctcata aaaaacataa ggggaacact atgaaagagc ttttcaaaca atggctaatc
     9601 aatcaggact cgccattcat taatagctgt ggcgttgagt gtattttgtc aaaagttgat
     9661 gactggctaa acataatcaa cgccaacgaa gaagaaaccg aaacgcttgt tgagtggcta
     9721 aatgcttttc tgcaagaggt ttctgttttt gtggcttagg tactataaat cttctgcttt
     9781 cacaaacacg ccatcaatca tttttccttt tcgatttttg atttgaatat aagcatagag
     9841 gagagctgtt tgtacgtctg ttttgtagaa ttgagcgata agatcaagct gattttcaaa
     9901 gccgcttaag taatcttcta atcgagataa atcacaacct ctagataact cacttgctac
     9961 attacctaaa tcaatattgg cctgaacaat ctgagccgca actccacaat taaaattggt
    10021 attatcgcaa tggctaatgc taaacagttt ttccattttg agctgtttac gcataatcgt
    10081 acaaatcact agacagtcac ctagactgtc taaaatagtt tcaaattcat tggttgctac
    10141 acctgcacat aattccccaa actcttccat tagttttaac atttgctttt gtggggtaga
    10201 gccgttaatt aaattgcggt cttccgccca ttgttcgatg tttttaatta gttgttgcat
    10261 tgtttagtcc tctcatttct ttcattgctt catcaatcgc cgtgcgtaaa tcggcatttc
    10321 ttgccatacg gaaatgctgg ctgttttcag cgtgattact aataacaaat tcgtctgata
    10381 agccattgta gcggatttcc attttgtgct ttttgataaa atctaagcgt ttggtgtctg
    10441 tcattggttg tcctttggca gctttggaag tggttgccag tgggttactg caatcacatc
    10501 gtcatcaagc ccatattgat ttacactaag aaaataccac tcgttatcgg caagttctct
    10561 tgaaactatt aaaattaact gcattcctga aatatcgcat aaccctagca catccgtttc
    10621 aataggtggt aatttgtcct caatggatat ccagccatta ttttctacaa gtgttggtgg
    10681 ttcaataact tgttcaactt ccccaaataa ctctgattct ctttcttcgt cggttagtgg
    10741 gcggatatct gattttgctc tacctaaaac aacaccataa actgcatagg gtaaatcata
    10801 ggcttcatag ctttcgtgat catccatgtc acccgcaaac tcataggctt cagtagcacc
    10861 atttaaacag ctttgttttg cttgctctaa ggtttcgtgc agatttagga tatgtatatc
    10921 atttgataca tccaccgaaa aaaatttgtc ttcattcatt ttctttctcc aaaataccaa
    10981 tagttgagag aataataacc gcttgtattc aacaagcggt caattttcat taaaaactta
    11041 caagtaaact tataagctaa cttacaagct agatctcaat cgtaccgata ttgaccttga
    11101 ttgatgtttc gctaagtgca tcggatagtt tttccgcaaa ctcagtcgca atcgcctctt
    11161 caatttgctc agcttgaact aagcgggcgg ttaataccgg ctcaccgcta ccggttagca
    11221 cctgtacacg cagagtaaat gatcgagtgt ctaagccgtt atatgggttg caagtaaata
    11281 caaggtgagt tggtagttgc agtgtagatt tagcttccac cgattccatt gctgattttt
    11341 tggctgcgaa atcgctgagt tcatgttctt cgtttcgggc gtagtcaatc gtcattttac
    11401 gcaccgcttg gacggcattt ttaatactga tttcatcttc gtggtggtaa gcggtcaagt
    11461 agccgcccca atcttcgagg aaatcagaaa aatcacgctg tgagcggcgt gagccattaa
    11521 tttcgcatag agctttgtag gctgcggttt tcttcatcac caaattagca cgatgtttag
    11581 cgtgaccggc ttgctctcgg ttgcccatat cgaatacaat ttccgcactc attttctctt
    11641 ggtcaataaa gcattgtgcg ttttcttggg cattggcggt agcatattca atcaggctac
    11701 caaaacgttg cgttgaaaac gctgcacgga attgattacg gtgagcttgg aaaggctcta
    11761 aattttgtaa tttaatgcct tcaggtaaca ttactgtgcc aaaatcagtt tgaacctctt
    11821 tagtcgcagc tacagcaaga gtagagattt gttgaatagt tgatttatcc attttgagtt
    11881 cctttttgtt taagtaaaaa taaagccacg cattaacgtg gcttggattg aaaataattg
    11941 attatgcagc agctttagag agagccttta aaccgctagg gctttctttt actgccgttt
    12001 ctgttttttc aggtgtagca gataatgccc cacctttatg aacatacatt ggtgtggcgg
    12061 tagtatcttc ttcggctgac ttaccacgtt tggtcggttt gacatagctc aatttgtgct
    12121 gaatttgaac agatgggctg tcgctatcca ttttgtctaa gctaaattca accgtaactt
    12181 tccctttctt atcgtgggtt agtacgccta acgccacttc tgaaagagct gttgccagct
    12241 tgtctttgaa aataccgcaa tctaattcat ctaagaattc aggtacattt gtttttgcca
    12301 ttttgggttc tcctaattag ggtttgaata aaattattag aacggaatat catcatcaaa
    12361 atctcgtgat tctgaatccg tcattacgtg gttatatgga ttggttggcg gtgggtttcc
    12421 tgccggttcg gttgcccaat tattattgct gtttccgctg cttgagctac caagcatttg
    12481 tagctgatca gcgatgattt cagtcgtcca gcggtctgtg ccgtcttggg cttgccattt
    12541 gcgagtacgt agtttgccct caacatagac ttgtgagcct tttttcaggt atttgccaat
    12601 aatgtcagct aaattccgaa aggcgataat gctatgccac tctgtggctt gttttttatc
    12661 gcctgatact ttatctgtcc attcggtcga agttgctacg ctgattttgg cggttgtatc
    12721 gccgtttggc attgttcgca attccgggtc gttacctagg cgaccgacaa taatcacttt
    12781 gttaattcct gccattgtta ttccttataa agtttaattg gtttatctct gccaacgttg
    12841 aaccgctcaa ttttgcggtt aatcaacata attgcgtttt ggcggctgta ttgtggatag
    12901 gttgtgtgct gtaatttgcc gtcataaaaa aacttggcga taaatagttt atctacctca
    12961 acaatctcaa caccggcatc aatttttcgt gccatatttc cctccgcagt attgattgcc
    13021 attactctca acttgccaaa cgccttgatt tcgttcgcat ttgtaacgtt gcatttgtat
    13081 gtggtcgtca taatccattc gcccgacaat gcctaatagc aaaattagca aggcaaatgg
    13141 ggcaagttgt ttcattttgt tcataggatt gtcctttagg gttaaaaaaa gcccacgttt
    13201 tacggtgggc aaatggagcg acttgcgtct agttgtgggt attccggaac acacttggtg
    13261 aaatatgctt tggaataagt gcctttcttt atacttgtaa ggctcaagtc ccttattgcc
    13321 tctcacaact ctaaggaata taatcaaggc tctcacaact caaataagga ttaagttatg
    13381 aaaaataata ttttaattac ttatgattta aataaatcag ggcaaaatta cgcagcactc
    13441 attgaaaaaa ttaaaactct tggagcttgg gctaaagtac agcagtcagc ttggtatctt
    13501 catacttcat attcctcaaa agaggtttta gaaatgctaa gcaccgtaac tgattacaat
    13561 gactctcttt ttgttgctca attatccgag gctaattgga gaggattatc caatgaggtt
    13621 agtaaattta ttcaagaaca atggtggaaa tagacctatt cgcaacttcc attacctgta
    13681 tttattccgc ttttattgat tgtttcaatt gcagaacaac atcgtgatgc aagcatttct
    13741 gggtccaagc aaccattttc tacagcttta agtacagcct gtttgatatg gtatttatcc
    13801 tcatcagaca ggccattctc tacaaccttt aattccaaat ctttagggct tcctagttgt
    13861 tcatttaaaa taacaacttt cgcaatttca aaatttttgt ttttcataat tacctcgtgt
    13921 ttaaattatt ccaaaaccca ctcataatag gctttggaat aaatgcgtgt ctgcccacgc
    13981 cgtcgcccgc cctgcatttc gcctgttcag tcgggtgctg cttgtatccg tacttactca
    14041 aacttgagat aagcgtgatt gcttaggact gataccagtg ttgccttttc agctctccac
    14101 accttgcgag tgtgttgcct aaaccttgcc acctctgttt ggcaagtcgt tcaaatttgt
    14161 atgtcagggt ctaaccaacc ttgccgagtg ataaagcatt actttacaac tgctcgaggt
    14221 tgttaatatc gtccgcacaa gcactaaatt gttaaagaac atcccacagt ctctgcttat
    14281 ctctcggcta ttgcctgcta tgtgggctag gtctagaacc tttattcaag ccctcagtaa
    14341 agggctttga taaaaattct tagtgaatgt aggcttgcca aagttgttga gcttgttctg
    14401 cgttcatttt gtcgctttgg ttccagtcga ttaacttgcc atttctgaac attgctcttt
    14461 caatcacaaa ctcttgcgtg gtttcatttt caaagacctc aaacacattg atgaaatcac
    14521 tagtgcgttt ttctttacgt ccgcataaaa tgtagtcttt gtgg
//
LOCUS       DANLZC010000057        14231 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.057, whole genome shotgun sequence.
ACCESSION   DANLZC010000057 DANLZC010000000
VERSION     DANLZC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14231
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.057"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(24..809)
                     /gene="znuB"
                     /locus_tag="PXE44_001314"
     CDS             complement(24..809)
                     /gene="znuB"
                     /locus_tag="PXE44_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDL5588827.1"
                     /translation="MFDILFPAWLAGVLLAFITAPLGAFVVWRKMAYFGDTLSHSALL
                     GVALGIFLELDPYLAVIGMTILLALLLVWLEQKTNYSVDTVLGIIAHTSLSLGVITIS
                     LLDNVRVDLMSYLFGDLLAINFSDVVFIGIGATLICLILMLFWKRLLSITISPELAQI
                     EGLNVARLRLLLMILTALTIALSMKFVGALIITSLLIIPSATARRFAKTPEKMVLIAI
                     FFSIIAVSGGLLLSSWKDTPAGPSVVICAGLIFLLSLFKKEYH"
     gene            complement(896..2233)
                     /locus_tag="PXE44_001315"
     CDS             complement(896..2233)
                     /locus_tag="PXE44_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL5588828.1"
                     /translation="MSSLSFDFSEDFRPLAAKMRPRNLAEYVGQSHLIGEGKPLRRAI
                     EAGHSHSMIFWGPPGTGKTTLAEIIAHHLDAEVERISAVTSGVKEIREVIEQAKLNRQ
                     AGRRTLLFVDEVHRFNKSQQDAFLPHIEDGTIIFIGATTENPSFELNNALLSRARIYI
                     LKPLQAVEILKILQMAIADTERGLGNETLVLEDDVLALLADYVNGDARFALNCLELMV
                     DMAQDSAKGKVLNKSLLTEVLGERQARFDKGGDRYYDLISALHKSIRGSSADGALYWY
                     ARILTAGGDPLYVARRLLAIASEDIGNADPRAMQIAIVAWDCFTRVGAYEGERAIAQA
                     IIYLAVAPKSNAVYQAFNEAKRLAKEAKDYDVPAHLRNAPTQLMKSLGYGEEYRYAHN
                     EPNAYAAGENYFPPELKDTEFYFPSERGMEKQIKEKMAWLKAQDQASPIQRYK"
     gene            complement(2243..2731)
                     /gene="mreD"
                     /locus_tag="PXE44_001316"
     CDS             complement(2243..2731)
                     /gene="mreD"
                     /locus_tag="PXE44_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL5588829.1"
                     /translation="MRTNPIFQILVIIAIFVVAFVLEIMPWPVGFQGLRPTWVVLVLI
                     YWALALPDKMSVGTAFIAGIVWDLILGSILGIHALVLSVAIYFVAKYHLILRNLSLWL
                     QSLLVMAYIVLIRFFIFVIEYILHSAEFNSQELLGAVISGVLWSWIFLLMRHIRRQLR
                     LR"
     gene            complement(2731..3786)
                     /gene="mreC"
                     /locus_tag="PXE44_001317"
     CDS             complement(2731..3786)
                     /gene="mreC"
                     /locus_tag="PXE44_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL5588830.1"
                     /translation="MKPIFAKAPSLGMRLIIAVIFSIMLIISDGRSSLMIQARNLLET
                     AVSGLYYFANTPRSILDGVSGNFIDNNKLQLENNLLKEQLREKSADLLLLDQLKVENQ
                     RLRLLLSSPLRQDEYKKIAEVLAAEMDAYRQQVVINQGRLDGAFVGQPIIDERGVVGQ
                     VISVGEKSSRVLLLSDITHAIPVQVLRNDVRGIASGTGVNNELVIDNLPRSIDVVKGD
                     VLVTSGLGGRFPEGYPVAIVETVENDTKSQFARIVARPLASFDRLRYLLLLWPTAEEK
                     NYSQSLSPAQVREVVEERRNSVNPFERLKQMSNKKTEVQLEAKEEQIENDEINENPAA
                     QPEAENEQNYHTEEGAE"
     gene            complement(3888..4940)
                     /locus_tag="PXE44_001318"
     CDS             complement(3888..4940)
                     /locus_tag="PXE44_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDL5588831.1"
                     /translation="MFKKFLGLFSNDLSIDLGTANTLVYVKGQGIVLDEPSVVAVRQD
                     RMGSLKSIAAVGSHAKLMLGRTPKSIMAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPAGATQVERRAIKESAIGAGAREVYLIEEPMAAAIGAGLPVHEAMG
                     SMVIDIGGGTTEVAVLSLNGIVYSTSVRIGGDKLDEAIIAYVRRHFGSAIGEATAERI
                     KQEIASAKIESDDDVKTMEVHGHNLAEGAPRTFELNSRHVLEAIEQPLNGIVEAVKSV
                     LEQCPPELAADIFERGMVLTGGGALLHNLDRLLSDSTGVQVVVAEDPLTCVARGGGKA
                     LEMIDMHGGDVFSIDD"
     gene            complement(5055..5684)
                     /locus_tag="PXE44_001319"
     CDS             complement(5055..5684)
                     /locus_tag="PXE44_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDL5588832.1"
                     /translation="MDIFIQGFIVCFGLIVSIGAQNAFLLKQGILKQHIFWIALLCFL
                     GDVFLMTIGVLGLGTLIAELPVVSFIISLLGAIFLLSYGSRSFISVFKSGEYLVASGE
                     NATSLKKALLITFAITFLNPHVYIDTVVIVGSIGGKLDFNGKIYFLAGALLCSFIWFF
                     GIGYGASLLSPYFAKRRTWQILDAVTGLIMYFIAFSLLIYTYQLALQIF"
     gene            5788..6435
                     /gene="udk"
                     /locus_tag="PXE44_001320"
     CDS             5788..6435
                     /gene="udk"
                     /locus_tag="PXE44_001320"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL5588833.1"
                     /translation="MTDTSQTSSCVVIAIAGASASGKSLIASTIYKELKNELGTDNIG
                     IISEDAYYRDQSHLTMEERIKTNYDHPNSMDHHLLVEHLRQLKQGNAIEIPEYDYTEH
                     NRKTTTKHFEPKKIIILEGILLLTDENIRNEINVSIFVDAPLDICFIRRLQRDMVERG
                     RSMESVISQYRKTVRPMFLQFIEPSKQYADIIVPKGGKNRIAIDILKAQIKQLLK"
     gene            6450..7031
                     /gene="dcd"
                     /locus_tag="PXE44_001321"
     CDS             6450..7031
                     /gene="dcd"
                     /locus_tag="PXE44_001321"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDL5588834.1"
                     /translation="MRLCDTDIKRYLDEGIIAITPRPSDDKISGATADVRLGNSFRVF
                     REHKTPYIDLSGPREEVAAQLNKVMSDEIIIGDDEAFFLHPGDLALATTLESVSLPAN
                     IVGWLDGRSSLARLGLMVHVTAHRIDPGWEGKIVLEFFNAGKLPLALRPNMAIGALSF
                     EILSGDAAKPYNARKDAKYKNQQSAISSRINQD"
     gene            7109..7264
                     /locus_tag="PXE44_001322"
     CDS             7109..7264
                     /locus_tag="PXE44_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDL5588835.1"
                     /translation="MLHYAVVFFVIAIIAALLGFGGIAGSATEIAKILFFVFLILTVI
                     SFFFKKK"
     gene            complement(7393..7995)
                     /locus_tag="PXE44_001323"
     CDS             complement(7393..7995)
                     /locus_tag="PXE44_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5588836.1"
                     /translation="METLIEFFSSYGYWAVFLVLLACGFGVPIPEDITLVSGGVISGL
                     GYTNVHWMLVVSMLGVLVGDSTMYWLGRIYGEKILKFPLIRNVATPERFATVQERFEK
                     QGWKLLFMARFLPGLRAVVYLVSGITRKVSFVKFLLVDFCAAIISVPIWVYLGDYGAS
                     NLDWLKEQVANGQHLIFAILGIVILFLGWKWYKNKKQYKK"
     gene            complement(7997..9016)
                     /gene="purR"
                     /locus_tag="PXE44_001324"
     CDS             complement(7997..9016)
                     /gene="purR"
                     /locus_tag="PXE44_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDL5588837.1"
                     /translation="MATIKDVAKYAGVSTTTVSHVINKTRFVAEDTTKAVWEAIKALN
                     YSPSAVARSLKINTTKSIGMIITTSESPFFAEIVLAVEEFCYSKGYSLFLCNTQNKLE
                     KIQNHLDMLIKKRVDGILVMCSEYTANSFDIFENSNIPMVIMDWGRSDERSDIILDHG
                     VEGGYIATQHLIENGHQDIAVITGNRDKEIARTRFEGFKKALSEAGLSIREEWIQEGD
                     FEPEGGYECMNNLLKSSEKLPTAVFCFNDSMALGAISAITEKGLNVPQDISVIGYDNV
                     HSSRFYAPPLTTIHQSKSRLGSAALELLLERIEDNENAKEPKVLEFFPELVKRKSVRN
                     LNIKE"
     gene            complement(9152..9880)
                     /gene="cmoA"
                     /locus_tag="PXE44_001325"
     CDS             complement(9152..9880)
                     /gene="cmoA"
                     /locus_tag="PXE44_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDL5588838.1"
                     /translation="MQRDTLFSAPIEKLGDFTFDESVAEVFPDMIQRSVPGYSNIITA
                     IGMLAERFVTDNSNVYDLGCSRGAGILSIRRNVNAKNVQIIGVDNSQPMVERCKTHLS
                     AYHSEIPVTILLDDIRHIEIKNASMVVLNFTLQFLPREDRLALLQKIYQGLNSNGILV
                     LSEKFTFESEEMNELLIDLHHTFKRANGYSELEVSQKRNALENVMRTDSIETHKKRLQ
                     EAGFTQVELWFQCFNFGSMIAIKA"
     gene            complement(9890..10672)
                     /locus_tag="PXE44_001326"
     CDS             complement(9890..10672)
                     /locus_tag="PXE44_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL5588839.1"
                     /translation="MTEKSQLNFNYQPSNNHANAQTMIFLHGLFGDLNNLGAIAKPFA
                     ERYSILMVDLRNHGRSFHSDEMNYALIAQDLAQLLTELNIQNAIVVGHSMGGKSAMAL
                     AAFAPELVEKLVVIDIAPVTYTQNRHQQVMAALFAVKEAKPQNRQQAKAIMAEYIRDE
                     GVQQFMLKSFDAQSEQSFRFNLQALKDNYQNVMGWQQVFVDKPTLFIKGALSDYIQAE
                     HTQTILAQFPQAKSFIVANADHWVHAEKTGTVVRAINKFLEK"
     gene            10791..11357
                     /gene="seqA"
                     /locus_tag="PXE44_001327"
     CDS             10791..11357
                     /gene="seqA"
                     /locus_tag="PXE44_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDL5588840.1"
                     /translation="MKRIEIDDELYQYIASRTQSIGETASDILRRLLRLPQSPQPFVL
                     VQEHMINELKELVKTPSRATKKNESNAEKTVEKLENILNSEHFMNENKNVVRFTMLLA
                     ALYRANPEAFAKATEKVRGNERIYFSQSEAEILATGSGVKAKQIPDSPFWVITNNNTA
                     RKGLILKGVMESMKVPSQLIERIQALFI"
     gene            11367..12785
                     /gene="menE"
                     /locus_tag="PXE44_001328"
     CDS             11367..12785
                     /gene="menE"
                     /locus_tag="PXE44_001328"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDL5588841.1"
                     /translation="MLINSTRFPTQFWAAHSAQNVAIIWEKGTSDYLNHLPSNITWYS
                     LNQLIAQAIFVLQQQGATPHSLIAYCGSHRLIGLLTYCASLALGAKILMLNPAQTASQ
                     RQAVLDDNGVELIINDADFAKFSAKTTACNAFPTIDFEKPATLTLTSGSSGKPKAVVH
                     SIAAHLYSAEGVCELMAFEQPNSWLLSLPLFHVSGQGIIWRWLFKGATLYVHEDKSDF
                     FLSAQKVSHLSLVPTQLQRYLNQLDSMVSQKCLLGGSMLPAELIAQAQQSGMTTFSGY
                     GMTEMASTICAVENDLNSVGFPLKHRELKLENGEIWVKGKSLGLGYWQKNGQIRPLVN
                     TQGWFATKDRGEWNHKKQLVIKGRLDNMFISGGENIQPEEIEQLIFRSQLVEQIIVLP
                     IEDKQFGHRPVAFLQFKQSDSKNELHKLKIWLSDKLERFKQPVAYYLLDIEKYQKQGL
                     IKLSRTQLQQDLKLKVLKEIYV"
     gene            12778..>14231
                     /locus_tag="PXE44_001329"
     CDS             12778..>14231
                     /locus_tag="PXE44_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDL5588842.1"
                     /translation="MFRPILKTFVLSLGLMLNSYALAELPTQQAVQTQLDSAKKLDQA
                     DADNKALVQTLEDTLAFLAQIDKQKADNQALNKSIQDSQKALKTSQHNLEKLKEVTIA
                     SVDNLTQKATTDLQKELTSTQSQLETVQHDLSIINNNLVAQNTAPDKAQTALTEMVKR
                     KQEISSQLSATNIKNELQTKLEVELELIELKNTYNQILLGGSEDLSALYSAQLDEKKQ
                     EQQNLQAEIANLQTAINNKIVEESQNKLDQAQAVQNQQNNATTNPVILRELDINTKVT
                     QELLKQTKDMTQLSQDNLRIKSVLDNLQQTQRNIEEQISSLQGTLVLSRIINKQKQSL
                     PQDEMISGLSKQIADLRVRVFDITEFKDSFADINAYISRIEQDEKTTFTSKEKEQLSK
                     ILQERSDTLTEMIKSLNNQLNLLINIELNQQQVQTISDALQQKLQQQSFWVKSNNPID
                     LEWFSKFVDITHYQFIDIGKKLNFSNWRDNLLPA"
BASE COUNT         4652 a         3014 c         2461 g         4104 t
ORIGIN      
        1 tttattttca tacattatgc tgtttaatgg tattcttttt tgaatagcga taatagaaaa
       61 atcaagccgg cacaaatgac taccgaaggc cctgctggtg tatctttcca actggataac
      121 aataaaccgc cactgactgc aataatactg aagaaaatcg caatcaacac cattttttca
      181 ggcgttttag caaagcgtct tgccgtagca gacggaataa tcagcagtga ggtaataatt
      241 aaagcaccga caaatttcat acttaatgcg atagttaatg cggttaaaat catcagcaat
      301 aaacgtaagc gagccacatt taatccttca atttgagcaa gttccgggct gatggtaatc
      361 gacaacaacc gcttccaaaa cagcattaaa atcaaacaaa ttagggttgc tccgattccg
      421 ataaacacca catcagaaaa attaattgcc agtaaatcgc cgaataagta agacatcaaa
      481 tccactcgta cattatccaa caaactaatg gtaattaccc cgagagaaag gctagtatgg
      541 gcgataatcc ccaacacggt atccaccgaa taattggttt tttgctccaa ccaaaccagc
      601 aataatgcaa gaagaatagt cattccaatt acagcaagat acggatcgag ttctaaaaaa
      661 atccctaatg ccacgccgag caatgcagag tgcgaaagtg tgtcgccgaa atacgccatt
      721 tttcgccaca ccacaaaagc ccctagaggt gcggtaataa aggctaacaa aacaccggca
      781 agccaagccg gaaataaaat atcaaacata ttggttctca aaggcaagta aaacaagcgg
      841 ttattttttc ggaaaaattt gcaaattttt tgctgattta gaccgcttgt catatttatt
      901 tataacgttg aatcgggctg gcttgatcct gtgctttcag ccacgccatt ttttctttaa
      961 tctgtttttc cattccccgc tcacttggga agtagaattc ggtatctttc agctcaggcg
     1021 ggaaataatt ctccccggcg gcataagcgt ttggctcatt gtgggcatag cgatactctt
     1081 cgccatagcc taaagatttc attaattgag tgggagcatt acgcaaatgc gccggcacat
     1141 cgtagtcttt tgcctcttta gcgaggcgct ttgcttcatt aaaagcttga tagaccgcat
     1201 tgctctttgg ggcaactgcc aaataaatga tcgcttgggc aatcgctctt tcaccttcat
     1261 aagcccccac tcgggtaaag caatcccaag ccacaatcgc aatttgcatt gctcgagggt
     1321 cggcattacc aatatcttca gaagcaatcg caagcagcct tcttgccaca taaagcggat
     1381 caccaccggc ggttaaaatt cgggcatacc agtataatgc tccatcagcc gatgagcctc
     1441 gaatggattt atgtaacgca gaaatgagat catagtaacg atcaccgcct ttatcaaaac
     1501 gagcttgtcg ttcgcctaac acttcggtca gcaaactttt attcagcact ttccctttag
     1561 ctgaatcttg tgccatatct accattaatt ccaaacagtt caaggcaaaa cgggcatcac
     1621 cattaacata atcagcaagc aatgccagta catcatcttc aagtaccaaa gtttcattgc
     1681 ctaaaccacg ttcggtatcg gctatcgcca tttgcaaaat ttttagaatt tcgaccgctt
     1741 gtaacggctt taaaatataa attctggctc gggaaagcag tgcgttattt agctcaaaag
     1801 agggattttc ggtagtcgca ccaataaaaa taatagtgcc gtcctcaata tgcggtaaaa
     1861 acgcatcttg ctggcttttg ttaaagcggt gaacttcatc cacaaaaagc aacgttcgtc
     1921 tgccggcttg gcggtttaat ttggcttgtt caatcacctc tcggatctct tttacaccgc
     1981 tggtcacagc ggaaatacgt tcaacttccg catcaagatg atgggcgata atttcagcta
     2041 gggtcgtttt tccggtgccg ggcggtcccc aaaaaatcat agaatgacta tgtccggctt
     2101 caatcgctct gcgtaacggt ttgccttcgc caattaaatg agattgcccg acatattcag
     2161 ccaagtttcg aggtcgcatt ttggctgcaa gcggtcgaaa atcttcacta aaatcaaaag
     2221 acaaactact cattttaacc tcttaacgta accgcagttg gcgacgaata tgacgcatta
     2281 ataagaaaat ccaagaccaa agcacaccac tgattacagc acctaacaat tcctgtgaat
     2341 taaattcggc actgtgtaaa atgtattcaa tcacgaaaat aaagaaacgg atcagcacaa
     2401 tatatgccat tactaatagg ctttgtagcc atagcgataa gtttcgcaga atcagatggt
     2461 atttcgcaac aaaataaatc gccaccgata acactaaggc gtggatgcct aaaatcgagc
     2521 caagaatcag atcccagacg atgccggcaa taaaagccgt ccccacactc attttatcag
     2581 gtaatgccaa tgcccaataa attaacacca aaacgaccca agtagggcgt agcccttgga
     2641 atcctaccgg ccaaggcata atctcaagta caaaagcaac cacaaaaatc gcaattatca
     2701 caaggatctg aaaaatcggg tttgttctca ttattccgct ccttcttccg tatgatagtt
     2761 ttgttcattt tccgcttctg gttgtgctgc cggattctca tttatctcat cattctctat
     2821 ttgttcttct tttgcctcta gctgcacttc agttttcttg ttagacatct gttttaaacg
     2881 ttcaaatgga ttcacgctat tacgtctttc ttcaacaact tcacgtactt gcgctggcga
     2941 caagctttgg gagtagtttt tctcttccgc cgtcggccaa agcagtaata agtaacgtaa
     3001 gcgatcaaat gaggctaacg gacgagcaac aattctggca aattgacttt tggtatcatt
     3061 ttcaaccgtt tctacaatcg ccaccggata tccctcaggg aaacgtccac ctaagcctga
     3121 agtcaccagc acatcacctt ttaccacatc aatagaacga ggcaagttat caatgaccaa
     3181 ttcattattc acccctgtac cactggcaat gccacgtaca tcattgcgta gcacttgaac
     3241 aggaatagca tgagtaatat cagagagcaa taaaacacgg ctagattttt cccctacaga
     3301 aataacttgt ccaacaacac ctctttcatc aataataggt tgcccgacaa aagcaccgtc
     3361 taaacgccct tgattaatca ctacttgctg acgataagca tccatctcag cagctaatac
     3421 ttcagcaatt tttttatact catcttgccg aagcggagaa ctcaataaca atcttaaacg
     3481 ctgattttca acctttagct gatcaagcaa taataaatcg gcactttttt ctcttagctg
     3541 ctcttttaat aaattattct caagctgtaa tttattatta tcaataaaat tgccacttac
     3601 cccatctaaa attgaacggg gggtattagc aaaataatat aatccactta ccgcggtttc
     3661 taatagatta cgcgcttgaa tcattaaaga gctacgccca tcggaaataa tcagcataat
     3721 agaaaatatc actgcgataa tcaggcgcat ccctaaagag ggagctttag caaaaatagg
     3781 tttcattaga gaaatatatg tgaatttatt gggaatctat aacgaaaatt gacaatagaa
     3841 tcaatccatt gccaacctcc atttaaacag ctgctaagag aaatgtatta atcatcaata
     3901 ctaaatacat caccaccgtg catatcgatc atttctaatg ctttaccacc accacgagct
     3961 acacaagtta atggatcttc tgctactacg acttgcacgc cagttgaatc agaaagtaaa
     4021 cgatctaaat tatgtaataa tgcaccacct cctgttaaca ccataccgcg ctcaaaaatg
     4081 tctgctgcca attcaggtgg gcattgctct aagactgatt taactgcctc tacaatacca
     4141 tttaatggtt gttcaattgc ttctaataca tgtcttgagt taagctcgaa agtacgagga
     4201 gcgccttcag ctaaattgtg tccgtgtact tccattgttt taacatcatc atcggattca
     4261 atcttagcac tagcaatttc ttgtttaatt cgctctgccg ttgcttcacc aatagcagaa
     4321 ccaaaatgac gacgtacata agcaataata gcttcatcta acttatcacc accaatacgc
     4381 acagaagttg aatacacaat gccgtttaaa gaaagcactg ccacttcggt tgtaccgcca
     4441 ccaatgtcaa ttaccattga ccccattgct tcgtgaaccg gcaaacctgc accgattgca
     4501 gccgccattg gctcttcaat taaatagact tcacgcgctc ctgctccaat tgcagattct
     4561 ttaattgcac ggcgttccac ttgtgttgca cctgctggca cgcacactaa tacacgaggg
     4621 cttgggcgca taaagttatt actatgtact tgtttaataa agtgttgcaa cattttttct
     4681 gttacaaaaa aatccgcaat gacaccatct ttcattggac gaatcgccat tatgcttttt
     4741 ggtgtacggc ctagcattaa ttttgcatgt gagcctaccg ccgcaatact ttttagcgat
     4801 cccatacgat cttgacgtac agcaaccact gagggttcat ctagcacaat accttgacct
     4861 ttaacataaa ccagtgtatt tgctgtcccg agatcgatag aaagatcgtt tgaaaataat
     4921 cctaaaaatt ttttaaacat aaaagaatcc gttaaaataa aattattcaa aaataagcac
     4981 attttatagt gcgaacattg ctactaatat agcaaaaaag cccgtcattc atcaatgaaa
     5041 aaatttaaac tccactagaa aatttgcaga gcaagttgat aagtataaat cagtaaacta
     5101 aaagcgataa agtacataat tagcccggta acagcatcta aaatttgcca agttcttcgc
     5161 tttgcaaaat aaggggaaag taagcttgct ccataaccta tgccaaaaaa ccaaataaat
     5221 gagcaaagta atgcacctgc taaaaagtat attttaccgt taaaatctaa tttcccaccg
     5281 atactgccta caatcacaac cgtatcaata taaacgtgag gatttaagaa tgtaatcgca
     5341 aaggtaatca gtaaagcctt ttttaagctc gttgcattct ctccacttgc cacaaggtat
     5401 tcaccacttt taaatacgct gataaatgaa cggctaccgt agcttaatag aaaaatggcc
     5461 cctaaaagcg aaataatgaa acttacaaca ggcaattccg caattaacgt gcctaagcct
     5521 agcacaccta tcgtcattaa aaatacatca cctaaaaaac agagcaatgc aatccaaaat
     5581 atgtgttgtt ttaaaatccc ttgctttaat aaaaaggcat tttgtgcgcc aatagagaca
     5641 attaagccaa aacacactat aaatccttga atgaaaatat ccattttttc tccaaaatcg
     5701 cataacaagc ggtcaattat acaaaaaatt ttgcaaaact tcggtataat ttatcgaata
     5761 ctcttttgac tgaataggaa attttttatg actgatacta gccagacttc atcttgcgtt
     5821 gtcattgcga ttgccggcgc atctgcctca ggtaagagcc tgattgcctc aaccatctat
     5881 aaagaactca aaaatgaatt gggaacagat aatatcggta ttatttccga agatgcttat
     5941 tatagagatc aatctcatct aacaatggaa gaacggatta aaaccaatta cgatcaccct
     6001 aattcaatgg atcatcattt attggttgaa catttacgcc aattaaaaca aggtaatgcg
     6061 attgaaattc cagaatatga ctataccgaa cataatcgta aaacgaccac gaagcacttt
     6121 gaaccaaaga aaatcattat tttagaaggt attttattgc tgacagatga gaatattcgc
     6181 aatgaaatta atgtttctat tttcgtagat gctcctctgg acatctgctt tatccgccgc
     6241 ttacaacgtg atatggtgga acgtggacgt tcgatggaat cagtgattag tcaatatcgc
     6301 aaaaccgttc gcccaatgtt cttacaattt atcgaaccct ctaaacaata tgctgatatt
     6361 attgtgccaa aaggtggcaa aaaccgtatt gccattgata ttcttaaagc acaaattaag
     6421 caattactaa aataaaatag gagctaaata tgcgtttatg tgatacagat attaaacgtt
     6481 acttagatga aggtattatt gccataacgc ctcgaccttc agacgataaa atttctggcg
     6541 ccactgcaga tgtgcgttta ggtaattcgt ttcgtgtatt tcgtgaacat aaaacgccct
     6601 atattgattt aagtggccct agagaagaag ttgccgctca gttaaataaa gtgatgagtg
     6661 atgaaattat catcggtgat gatgaagcat ttttccttca tcccggtgac cttgcactcg
     6721 caacaaccct tgaatcggtt tccttaccag ccaatatcgt tggatggtta gatggtcgtt
     6781 cgtcacttgc tagattaggc ttaatggtac acgttaccgc ccacagaatc gatccgggtt
     6841 gggaaggcaa aattgtattg gaatttttta atgctggaaa attaccccta gctttacgcc
     6901 ctaatatggc aattggcgca ttaagctttg agattttaag tggtgatgct gcaaaaccct
     6961 ataatgcgcg aaaagatgca aaatataaga accagcaaag tgccatttca agtcggatta
     7021 atcaagatta atatagtaca tgccttgcaa tcatacatta actatgttag ttttttaatc
     7081 agtttattac acagtaagga gtataactat gttacattat gcagtcgtat tttttgtcat
     7141 cgctataatc gcagcgctat taggatttgg tggtattgct ggaagtgcta ctgaaattgc
     7201 caaaattctc ttttttgtct ttttaatact tactgttatt tcgttcttct tcaagaaaaa
     7261 gtgacacagt taaacctaat tttttaagtt aaaatatagt tcaaaaaaac agttggtaat
     7321 ggatatttat aattattctt accaactgtt ttgctattat atctttttaa gatgaaatgc
     7381 tcgtcttttg tttcactttt tatactgctt tttattttta taccatttcc agcctaaaaa
     7441 tagaataaca atgcctaaaa tggcaaaaat gaggtgctgc ccattagcta cttgttcttt
     7501 tagccaatcc aaattagatg ccccatagtc gcctaaataa acccaaattg gcacagagat
     7561 aatagcggca cagaaatcaa ctaataaaaa ttttacgaag ctgacttttc tggtaatacc
     7621 tgaaactaag taaaccaccg cgcgcaagcc aggtaaaaaa cgagccataa aaagtagttt
     7681 ccatccttgt ttttcaaaac gttcttgcac ggttgcaaaa cgttcaggtg tcgcaacatt
     7741 cctgattaat ggaaatttga gaattttttc gccatagatt ctacctagcc agtacattgt
     7801 gctatcgcca actaatactc ctaacatact aacaaccagc atccaatgca cattggtgta
     7861 gcctaatcct gaaataacac caccggatac taacgtaata tcttcaggaa tcggcacacc
     7921 gaaaccacag gcaagtagca caagaaaaac agcccaataa ccatagctac taaaaaattc
     7981 aatcaatgtt tccatattac tccttaatgt ttaaattccg aaccgattta cgtttgacca
     8041 attcaggaaa gaactctaat actttaggct cttttgcgtt ttcgttatct tcaatacgtt
     8101 ctaataataa ttctaatgct gccgagccta agcgagattt tgactgatgg attgtcgtaa
     8161 gaggcggtgc ataaaagcgt gagctatgca cgttatcata cccgatcact gaaatatctt
     8221 gcggcacatt cagccctttt tcggtaatag ctgaaattgc ccctagtgcc attgagtcat
     8281 taaaacaaaa aaccgcggtt ggtaattttt cactgctctt aagcaaatta ttcatacact
     8341 catacccccc ctcaggttca aaatcgcctt cttgaatcca ttcttcacga atagacaaac
     8401 ccgcctcaga tagcgctttt ttaaaacctt caaaacgggt tcttgcaatt tctttatctc
     8461 tattgccggt aattacagca atatcttgat gcccgttttc aattaaatgc tgagtcgcaa
     8521 tatagccacc ttcaacaccg tgatctaaaa taatatcact gcgttcatca cttcgtcccc
     8581 aatccataat caccattggg atattgctgt tttcaaagat atcaaatgaa ttagctgtat
     8641 attcggagca catcaccaaa atgccatcaa cccgtttttt gatgagcata tctaaatgat
     8701 tttgaatttt ctcaagtttg ttttgagtat tgcacaaaaa tagtgaatac cctttgctat
     8761 agcaaaattc ctctaccgct aacacaattt cagcgaaaaa cggtgattcg ctggttgtaa
     8821 tgatcatacc gatagattta gtcgtattaa ttttcaaact ccgcgctaca gcactaggag
     8881 aatagttcag cgctttaata gcctcccaaa ctgcttttgt agtgtcctct gctacaaagc
     8941 gggttttgtt tataacgtga gaaaccgttg tagtcgatac accagcatat tttgcgacat
     9001 cttttatcgt tgccatacct ttttcctcct aaataagtag tggtgcatta taactgattc
     9061 taactgactt ttattataaa gaaatagata gaatgcaagc ggttgctttt tatcaagaat
     9121 ttgcaaaaaa ttttacaaat atcaccgctt gttaggcttt aattgcaatc attgagccaa
     9181 aattaaagca ttgaaaccaa agttcaactt gtgtaaaacc agcttcttgc agacgttttt
     9241 tatgggtttc aatactatct gttcgcatca cattttccaa agcattacgc ttttggctga
     9301 cttccaattc actgtagcca tttgctcgtt tgaatgtgtg atgaaggtcg atcaatagct
     9361 cgttcatttc ctcactctca aaggtaaatt tttccgataa cactaaaatg ccattggaat
     9421 ttaatccttg ataaatcttc tgtaataatg ctaggcgatc ttctcttggt aaaaattgca
     9481 aggtgaaatt aagtaccacc attgaagcat ttttaatttc aatgtggcga atatcatcaa
     9541 gtaaaatcgt caccgggatc tcagagtgat aagcacttaa atgggtttta caacgctcaa
     9601 ccatcggctg agaattatct accccgataa tctgcacatt cttcgcattc acattacggc
     9661 gaatagataa aatccctgcg cctcgtgaac aacctaaatc ataaacattt gaattatctg
     9721 tgacaaaacg ctccgccaac attccaatag cagtaataat attggaatag cccggcacgg
     9781 agcgttggat catatccgga aacacttcag caaccgattc atcaaaagta aaatcgccca
     9841 atttctcaat cggagcggaa aataatgtat ctctttgcat ttttctccct tatttttcta
     9901 aaaatttatt gattgctctc acaacagttc ctgttttttc tgcgtgaacc caatgatcag
     9961 cgtttgccac aatgaaagat ttggcctgcg gaaattgtgc caaaatcgtt tgtgtatgct
    10021 ctgcttgaat ataatccgat aatgcacctt taataaacaa ggtcggttta tccacaaata
    10081 cctgctgcca ccccatcaca ttctgataat tatcttttag cgcctgtaaa ttaaaacgga
    10141 aagattgctc gctttgggca tcaaaagatt tcaacataaa ctgttgcacg ccttcatctc
    10201 gaatatattc agccattatc gctttagctt gctgacgatt ctggggttta gcttctttca
    10261 ccgcaaataa ggctgccata acctgctgat gccgattttg ggtataagtg accggggcaa
    10321 tatcaatcac taccaatttc tccaccagtt caggagcaaa tgcagccaat gccatcgcac
    10381 tttttccgcc cattgaatgc ccaaccacaa tagcattttg gatatttaat tctgtgagta
    10441 attgtgctaa atcttgtgca atcaaagcat aattcatttc gtctgaatga aaagatcgcc
    10501 cgtgatttct gagatcaacc attaaaatgg agtaacgttc tgcgaatggt tttgctattg
    10561 cgcctaggtt atttaaatct ccaaacaatc catgtaagaa gatcatcgtc tgagcattag
    10621 catgattatt cgaaggttgg taattaaagt ttaactgcga tttttctgtc atataaatgt
    10681 cattgttcat ataaattttc agcgatgttc ctctataaaa aacaatagaa atgcgtataa
    10741 tagcaagcac tattaacgca tttaataccc gcagatattt gaggatcccg atgaaacgaa
    10801 tcgaaattga tgatgaatta tatcagtata ttgcaagccg tacacaatct attggtgaaa
    10861 ctgcgtcaga tatattacgc cgtctattac gcctgccaca atcgcctcag ccttttgttt
    10921 tagtacaaga acacatgatc aacgagctta aagagcttgt caaaacgcct tctcgtgcta
    10981 cgaaaaaaaa tgaatccaat gcagaaaaaa ccgttgaaaa attggaaaac attttaaatt
    11041 ccgagcattt tatgaacgaa aataaaaatg tagtgcgttt tactatgcta ttagccgcat
    11101 tatatcgagc aaatccggag gcttttgcta aagcaaccga aaaagtgcgt ggtaatgaac
    11161 gtatctactt ttcgcaaagt gaagccgaaa ttttagctac tgggagtgga gtaaaagcca
    11221 agcagatccc agattcaccg ttttgggtaa ttaccaataa taataccgca cgcaagggac
    11281 ttattctcaa aggcgtaatg gaatccatga aagtaccaag ccagttaatc gaacgcattc
    11341 aagcgctttt tatctaatta attgaaatgc tcatcaattc tactcgcttt cctacccaat
    11401 tttgggctgc tcattcagcc caaaatgtgg cgattatttg ggaaaaagga acatcagact
    11461 atcttaacca ccttccatca aacatcactt ggtatagttt gaatcagttg attgcgcaag
    11521 ctattttcgt gttgcaacaa caaggtgcta ctccgcacag tttgattgcc tattgtggtt
    11581 ctcatcggct cattggcttg ctcacttatt gtgctagcct tgctttgggg gctaagattc
    11641 taatgcttaa ccctgctcaa acagcaagtc aacgacaagc agtcttagat gacaatggtg
    11701 ttgagctcat tattaatgat gccgactttg caaaattttc agcaaaaacg accgcttgta
    11761 atgccttccc tacgattgat tttgaaaaac cagctaccct aaccctaacc tcaggctcaa
    11821 gcggtaaacc taaagcagtg gtgcattcta tagcggccca tctctatagt gccgaaggag
    11881 tatgtgaatt aatggcattt gagcagccaa actcttggct actgagcttg cctctgtttc
    11941 acgtttccgg acaaggcatt atttggcgtt ggttatttaa aggtgcaaca ctgtatgttc
    12001 acgaagacaa aagtgatttt tttctttcag cacaaaaagt gagccatctc tctttagtcc
    12061 ccacccaatt acaacgctat ttaaaccaat tagattctat ggtcagccaa aaatgtttac
    12121 tgggtggatc tatgcttcca gccgaattga ttgctcaagc acaacaatct gggatgacta
    12181 ccttttcagg ctacggaatg actgagatgg catcaaccat ttgtgcagta gaaaatgatt
    12241 taaacagtgt tggttttccg ttaaaacatc gggaactaaa attagaaaat ggtgaaattt
    12301 gggtaaaagg caaatcactc ggcttaggct attggcaaaa aaatgggcaa attcgaccgc
    12361 ttgtaaacac acaaggctgg tttgccacta aagatcgtgg cgaatggaat cacaaaaaac
    12421 agcttgtaat aaaaggacga ttggataata tgtttatttc aggaggggaa aatatccaac
    12481 ctgaggaaat tgaacaactg atttttcgtt ctcagcttgt cgaacagatt atcgtgttgc
    12541 cgattgaaga taagcaattt ggacatcgac cagtggcgtt cttacaattc aagcaatctg
    12601 attcaaaaaa tgaactccat aaattaaaaa tttggctgtc tgataaatta gaaagattca
    12661 aacagcctgt tgcctattat ttactggata tagaaaaata ccaaaaacaa ggcttaatta
    12721 aactatcaag aacacaatta caacaagatt taaaactcaa agtattaaag gaaatctatg
    12781 tttagaccga ttttaaaaac atttgtgctt agtttaggct taatgctaaa tagttatgca
    12841 ttagctgaat tgccaaccca acaggcagtg caaacgcagt tagatagcgc caaaaaatta
    12901 gatcaagccg atgcggataa taaggcattg gttcaaacac ttgaagatac gcttgctttc
    12961 ttagcccaaa ttgataaaca aaaagcagat aatcaggcat tgaataaatc tattcaagat
    13021 tcccaaaaag cattaaaaac aagccaacat aatcttgaaa aattaaaaga ggtgactatt
    13081 gcaagtgtcg ataatttaac acaaaaagcg acaacagatt tacaaaaaga gttaacctct
    13141 acacaatctc agctggaaac agtacaacac gatttaagta ttattaataa taatttagtt
    13201 gcacaaaata ccgcaccgga taaggctcaa acggccttaa ccgagatggt taaacgtaaa
    13261 caggaaatta gtagccaact ttcagcgact aacattaaaa atgaactaca gacaaagtta
    13321 gaagtagaat tagaattaat agaattaaaa aacacttaca atcaaatatt acttggtggc
    13381 agcgaggatt tatctgcgct ttactctgct caattagatg aaaaaaagca agagcagcaa
    13441 aatctacaag cagaaattgc aaacctgcag actgctatca acaataaaat tgtagaagag
    13501 tctcagaaca agctagatca agcacaagcg gttcaaaatc agcaaaataa tgcaacgacg
    13561 aacccagtta tcttgcgtga attggatata aataccaaag tcacacaaga gttgctaaaa
    13621 cagaccaaag atatgacgca gttatcacaa gataatttgc gaatcaaaag tgtgctagat
    13681 aacttacagc aaacccaacg taatattgaa gaacaaatca gttctctgca aggtacgttg
    13741 gtgctttctc gtattattaa taagcaaaaa caatcattac cacaagatga aatgatttcc
    13801 ggactatcaa aacaaattgc agatttacgt gtacgtgttt ttgatatcac tgaatttaaa
    13861 gactcttttg cggacataaa tgcttatatc agccgcatag agcaagatga aaaaacaacc
    13921 tttacgagta aagagaaaga acaactgagt aaaattttac aggaacgttc tgacacttta
    13981 actgagatga ttaaatcatt aaataatcag ctcaatttat taatcaatat tgaactaaac
    14041 cagcaacaag tgcagaccat tagtgatgcg ttacagcaaa aactgcaaca gcaaagcttc
    14101 tgggttaaaa gtaacaaccc cattgatctt gaatggttta gtaaatttgt cgatattacc
    14161 cactatcaat ttattgatat tggaaaaaaa ctcaattttt caaattggcg ggataattta
    14221 ttacctgcaa g
//
LOCUS       DANLZC010000058        13929 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.058, whole genome shotgun sequence.
ACCESSION   DANLZC010000058 DANLZC010000000
VERSION     DANLZC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13929
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.058"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            257..332
                     /locus_tag="PXE44_001330"
     tRNA            257..332
                     /locus_tag="PXE44_001330"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:290..292,aa:Trp,seq:cca)
     gene            349..534
                     /locus_tag="PXE44_001331"
     CDS             349..534
                     /locus_tag="PXE44_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588843.1"
                     /translation="MYNLDDAIQECREKFGEDKPYFSEAGTLTFAGCKALSRLRDEKL
                     GIKAPPNFPFPRVEDLS"
     gene            560..730
                     /locus_tag="PXE44_001332"
     CDS             560..730
                     /locus_tag="PXE44_001332"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588844.1"
                     /translation="MTEQLNTQSKSGEEDQEKSQDRYAVGWPSRRISDCVQHFRSRKI
                     YPFASNNDVKQG"
     gene            873..1118
                     /locus_tag="PXE44_001333"
     CDS             873..1118
                     /locus_tag="PXE44_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588845.1"
                     /translation="MKLMKDMGTQSYIWSGFSTWLAWLGEQQNLMLLSLAIGIVTAIA
                     GLIQRFDEWRRRKREDERAEELHQAKMARLRKGLSDE"
     gene            1111..1680
                     /locus_tag="PXE44_001334"
     CDS             1111..1680
                     /locus_tag="PXE44_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL5588846.1"
                     /translation="MSKSLKYGSGIVCGIAAIITLVQYQHPEIRTNQAGLEIIGNAEG
                     CRRDPYKCPADVITVGIGSTEFGGEKIDPNRIYSDKEIAERWAKDLKIAESCVNRHFN
                     GKDMNDNQFSGMTSAVFNMGCYNMRFYRNKQGQYVQTTIHKLAVNKQFEEMCHRLPDF
                     IRASGKVLNGLVIRREKEKALCLTGLVAR"
     gene            1653..2003
                     /locus_tag="PXE44_001335"
     CDS             1653..2003
                     /locus_tag="PXE44_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL5588847.1"
                     /translation="MLNWISGTIAIVILGLCVWLWGQSQMIDSLRAENRAQAQTIEQQ
                     QKANQRLTDSLEQERQAVEKIQKIANELRNKVEMAKNEITSILAQDSCAKADLPNGVA
                     DSIKRLHQQNNHKH"
     gene            2008..2181
                     /locus_tag="PXE44_001336"
     CDS             2008..2181
                     /locus_tag="PXE44_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588848.1"
                     /translation="MYPPAAYLVPCGRTEFSGRTYGDTVEYLVKVMGERDLCANQIDR
                     IREWQAQTKQGFK"
     gene            2573..3097
                     /locus_tag="PXE44_001337"
     CDS             2573..3097
                     /locus_tag="PXE44_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL5588849.1"
                     /translation="MTKKGEVKATSKGVGKLTDKQKRFVEEYLIDLNATQAAIRAGYS
                     EKTAYSIGEENLRKPEIRSAIQEAQNKRSERTQITQDDVLNGLLEVIAMSTGKKIVTE
                     TDVAKNDNGELVGFDIAKTKFEPAAANKALELLGKHLGMFKDKVDLINSDGSLNRPMV
                     IELIAPSLDDESTD"
     gene            3081..4301
                     /locus_tag="PXE44_001338"
     CDS             3081..4301
                     /locus_tag="PXE44_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDL5588850.1"
                     /translation="MKAQIELPPKLLPVFAKDYRYKGAYGGRGSGKTRAFAKMSAVSA
                     YKRAMRGESGVILCGREFMNSLEESSLEEVKQAIKSEPFLANFFDVGEKYVRTKDGRI
                     SYVFSGLRHNLDSIKSKARILIAWIDEAETVSETAWRKLIPTVRENNSEIWVTWNPEN
                     RGSATDTRFRQNPPENSCIIEMSYQDNPWFPDVLEQERLNDKQRLDDATYRWIWEGAY
                     LEASEAQIFRGKYQEMEFVPNPDFDGPYYGLDFGFAQDPTAAVKCWVFNGDLYIEFEA
                     GKVGLELDDTADFIAKGVPQIADHVVRADSARPESISYLKRHGLPRIIGVEKWKGSVE
                     DGIEHIKSYGKVFIHPRCQQTLNEFRLYSYKTDRLSGDVLPVVIDAHNHFIDALRYAL
                     TPLMQVKSAKGVLL"
     gene            4298..5674
                     /locus_tag="PXE44_001339"
     CDS             4298..5674
                     /locus_tag="PXE44_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDL5588851.1"
                     /translation="MSLEQDRLAFLAEALGLGNIKRRTLWKEFGYPNTLTFNHFLKAY
                     KRNSIAFAAINRLLDGCWVDCPVIVEGEQKNESKQTTEWESKVERFMKRYWSAIKEAD
                     RRNLVGNYSALLLQVRDGQQWDQPILQGALSSIGEFGLVKLIPVWQSQLSVTEFQEDV
                     TADNYGEPLMYQFSESAFGKKSVSRNIKVHASRVILLNEGGDFNSPHSGVPLLEAGYN
                     KLVDLEKTSGGSAEGFLKNASRQLGIKLTKDVDLRQLEDSAKALGFSSFSDALNDKIK
                     KINSGTDSALITHEGDASVLSVAPADPKPTWEISANEFAASVQIPFTILFGQQTGRLA
                     SDEDKTDWANRCNGRRNGFLTDVITQLLNRLWFIGVLPMPKNSDVTVSWSDLLAPSEK
                     EKIANAQALASVATTSQSAFGFAAVTANEIRESLGFEPLPDNLIPPTIDDENEDQDEN
                     QATADSDK"
     gene            5628..6566
                     /locus_tag="PXE44_001340"
     CDS             5628..6566
                     /locus_tag="PXE44_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL5588852.1"
                     /translation="MKIKTKIKPLRIPTNKADPLNMGVSVAKLFRHIDVIYSQIKRSI
                     KQQILQKMSLRLQANNFEYFPSYLTATELADLLETLQKIIDKEILSNGSQGENLWFDS
                     YIDEAALKGTQSAVTDLGLQSEAYRSQRNLSSIVFSPAYFNRLAIAHMASYSEWQGLS
                     DELRKSLAGVITEAVLNGKNVKETAREIRQKLDVSSKRAKRIAQSSQLAAYRRAEWQE
                     AEEAKEELGLNTKLLHFSALKATTRLAHAQRHGKYFDVAEVREWYSRDGNQFNCYCKQ
                     SVVVVNADGKTDIEPLLVGLAKERKQWVGALKNVKK"
     gene            6553..7923
                     /locus_tag="PXE44_001341"
     CDS             6553..7923
                     /locus_tag="PXE44_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDL5588853.1"
                     /translation="MSKNNVNIVTVINSKNISTESIDGDQHLIIRGVVPIVDDVVMND
                     GLYPADEINKSYNSLEGNFMPLGHPKIEGKYVSAQDVRAVNKHHVGAWAKNVRKESGK
                     VLVDMYINRRFAEGSEQGKTLLERLDGMVTNTNAEPIHVSTGLILNKVKQSGKSKGKK
                     YSWIATNMSFDHIAILLDVPGAATPDDGVGIFVNQDGSEMGVENVNLTQAADYRTEKL
                     FDKVRFYLMANSDLSFEEIHRALREAIRPEGKDKWSFWVEAVYPKYFIYSDDNSGKKY
                     KRSYFIDENDNVQLTSEPVEVVKKIGYEEITTNEEHNPMKTKILAVLNAANVKTEGLN
                     DDQLLEAYNKLQADKGKEPEKPKNEDDSEIDEKIKKAVNAAIEPLQQALQANTDKEQA
                     AMRAAVKAKFNMSDAAVNALQGEVLTELYAQTQQSVGLNNNSANGNGDNQWDGYSLNQ
                     EEKGNE"
     gene            7916..8350
                     /locus_tag="PXE44_001342"
     CDS             7916..8350
                     /locus_tag="PXE44_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588854.1"
                     /translation="MSNVIYRGSVKREPQTVNIVINDTSAPGAVVKLHNGKLEAAADL
                     KGRRFLLGNNRFKGQTIEQAYAKGDTATAFRLEPEQEYYAQIADGTYNFGDELTVKQA
                     GGKLTKAETGDVVLFFFDEESQRVISGGKGYADVVVANAYTK"
     gene            8365..9354
                     /locus_tag="PXE44_001343"
     CDS             8365..9354
                     /locus_tag="PXE44_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin"
                     /protein_id="HDL5588855.1"
                     /translation="MLKFTKEQERFVINERINYDKKHALMAANSAGLLGNAMTLPKDV
                     WAEWDRDAVQIQREELVVFNDLAGISKSMPIGKLIHHFQTVSDSGSVNISLDGRSSAK
                     TDNVVLNYHGTPLPIIDSTFSFGWRDMSAAQSEGYQIDGAARANSLRKVAEKMEDLAL
                     NGDSSIVVGNAKLYGLRTAPNRMTNTHNLDLATATPKEIYDVFRDLISKFHAKNYYSP
                     VTLYVNYGDYFAMSTRDYSEQKSEGSILNKVMTIPQIAKIVPASRVPQNEILGLCKRS
                     DVYQVLNGMPLVTRPIARHNETDDYSFQIMAAVAVEFKFDDKGNAGYIQYTKS"
     gene            9366..9653
                     /locus_tag="PXE44_001344"
     CDS             9366..9653
                     /locus_tag="PXE44_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588856.1"
                     /translation="MKWLLTHDSHELKKGDVYEGETLPLWLVGKAIEIEEKSFEVATP
                     SDHADGETGETGETGETGETGETGETGETGETGETGETGETGQKGKKDKGK"
     gene            9656..10033
                     /locus_tag="PXE44_001345"
     CDS             9656..10033
                     /locus_tag="PXE44_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588857.1"
                     /translation="MAAEIELSEARTTLDELGFTSPNSLLELYVQQTNTLDQALNKSG
                     YSEETQKLIKLYLVAILAISSGARRIKSEGAPSGASRTFEYDPNALDNLKSAIRSIDP
                     KGCAESLLPKDKVVGFFDVVGGC"
     gene            10033..10377
                     /locus_tag="PXE44_001346"
     CDS             10033..10377
                     /locus_tag="PXE44_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588858.1"
                     /translation="MSNLSNWSYTAKATLWQAKGKNDDGVLVFSTPQIISCDYGADRK
                     RARFEIGREQAVKNVIWTEFAHAKLGDYVLIGESEQADPILAGAEEIIHIQRFADTFD
                     RNRDDFALITGG"
     gene            10379..10747
                     /locus_tag="PXE44_001347"
     CDS             10379..10747
                     /locus_tag="PXE44_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL5588859.1"
                     /translation="MGIKVKGIRQVKQRLNQVVGEVQSQKATRAMFRILNAVAPLAAH
                     YTPVDTSTLINSQFTEMEVNGTRLIGRVGYSANYAAYVHDPNIKQNFRKPSAKKEFLT
                     SALNESKATIKSIVKEELSL"
     gene            10744..11124
                     /locus_tag="PXE44_001348"
     CDS             10744..11124
                     /locus_tag="PXE44_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588860.1"
                     /translation="MISYVKAFKQWLEAHSLADGYVVQLYQWEDTQKAKPVIVIQPNS
                     GSPQVSDLSSEHYLLISLVAGKNSGYTIEERARAIMSKILVEPFASFGYIESMGGLPA
                     PIFTEDNRMIFRLPLRIISTNQEE"
     gene            11128..11610
                     /locus_tag="PXE44_001349"
     CDS             11128..11610
                     /locus_tag="PXE44_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5588861.1"
                     /translation="MGSPSPQNNAENLVVGRAVVLEYGTTDAKPQESEWKVAGAMTTK
                     SWDFSPNSVTSEADDAGGFPETLITNSDFSISGDGEWRKRPKSKELGIKDLVVLYANA
                     VKNRTQPYIWVRLKYAGLTILGKMVITALSSEAPTNDLVKFSIEFKVGDSSTIEIAAA
                     "
     gene            complement(11650..12066)
                     /locus_tag="PXE44_001350"
     CDS             complement(11650..12066)
                     /locus_tag="PXE44_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5588862.1"
                     /translation="MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLL
                     SCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKVLLHNTMQEQAVSKAEIARLTNI
                     RPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG"
     gene            complement(12122..12298)
                     /locus_tag="PXE44_001351"
     CDS             complement(12122..12298)
                     /locus_tag="PXE44_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5588863.1"
                     /translation="MKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELK
                     TGLVEGVKKQLGLK"
     gene            12394..13065
                     /locus_tag="PXE44_001352"
     CDS             12394..13065
                     /locus_tag="PXE44_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588864.1"
                     /translation="MKMVSYPITEIGEMLIATDGRDFLFRPSFKNIYKIGSPQEIVKI
                     YALLHGCEIREILPHILTKSESHQSYFWSVLNSPVFGRKILQNAMIVMSCCCDDDISL
                     LIGEWLPSKSGVRYKLGKMPPETIINFAKNLMGHGVIGCCELPKKEQSAKNEFSESFD
                     AVEYISLARTHFNMSREDAENLTMTELQQLLKSQMPAENNSYGQFTEEEYDRIMAEYE
                     KRRAA"
     gene            complement(13080..13313)
                     /locus_tag="PXE44_001353"
     CDS             complement(13080..13313)
                     /locus_tag="PXE44_001353"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588865.1"
                     /translation="MKKDCTKQDFANWLNENQEVCWIKEVSIERILSCVDDYVAICNA
                     RSKDEFELLNDIRELFYSAHYKDFTPVLSGRFF"
     gene            13482..>13929
                     /locus_tag="PXE44_001354"
     CDS             13482..>13929
                     /locus_tag="PXE44_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588866.1"
                     /translation="MKKMIVFVASVLSSFVIASDWEIIEAAQNKVKAELIRKSEKKAE
                     LCLNVAQAVLITNQKVQKDTFSMCGEFSTEIVFSDSKAYSLAVCGKVKGKDIIGNPIE
                     SDYIYQRATKDFYFNYNGKKSAYYDPQSDLARLQEVSDSMFNAMQNR"
BASE COUNT         4227 a         2738 c         3321 g         3643 t
ORIGIN      
        1 aactgaaaaa atatcaattt tccccttgat tttagtgcgt aacaagtgta tattttgtgc
       61 taatggtgga cgtagtataa atgatgttta ccaatgttag ttaaagacgc agcctgaagt
      121 aatattcagt tcggaggaga ataactagaa acgacgtcga gtatagttat ttcagcgttt
      181 gcgtggcagt atgccgaccg aattagttta acagccctga gcagaaatgc ttggggcttt
      241 tttgtttacg gattttaagg gtgtagctca gttggtagag cagcggtctc caaaatcgtt
      301 ggttgttggt ttgagtccaa ccaccctttc caaatgcgag taaatgttat gtataacctt
      361 gatgatgcaa ttcaagaatg cagagaaaaa tttggtgaag ataagcctta tttttctgaa
      421 gctggaacac ttacttttgc tggttgcaaa gccctctctc gattaagaga tgaaaagtta
      481 ggaattaagg ctccgccaaa tttccctttc ccaagggttg aagatctatc ttagctacaa
      541 aaatcaatga gggattgcta tgacggaaca actaaatact cagtcaaaaa gcggtgaaga
      601 agatcaagaa aaatctcaag atcgctatgc cgtgggctgg ccatctcgac gtatatcaga
      661 ttgcgttcag cacttccgaa gtaggaaaat ttatccattt gccagtaata atgacgtgaa
      721 gcaaggttaa atgcttctgg tggtacagag taaattcaat catttttacc ctctgctagt
      781 ttatttgttg gggaacagta ttctagcaga atttttaacc aaagctcagt ctcacgactg
      841 ggctttttta ttgcctcgaa acggggtgga gtatgaagtt aatgaaagat atgggaacgc
      901 agagctatat ttggtcggga tttagcactt ggctggcgtg gctcggcgaa cagcagaatt
      961 taatgttact tagtcttgct atcggtattg ttactgccat tgcagggctt attcagcgat
     1021 ttgatgaatg gcgtaggcgt aagcgtgaag atgagcgagc ggaagagctt catcaagcga
     1081 aaatggcaag attgagaaag gggttaagtg atgagtaaaa gccttaaata tggcagtggt
     1141 atcgtttgtg gtatcgctgc cattattaca ttagtccaat atcaacatcc cgaaatccgc
     1201 actaaccaag ctggattaga gattatcggt aatgctgagg gttgcagacg tgatccgtat
     1261 aaatgccctg ccgatgtaat cacggttggc attggctcaa cggagtttgg cggtgaaaag
     1321 attgacccaa atcgtattta ttctgacaaa gaaattgctg agcgttgggc aaaagatttg
     1381 aagattgcgg agagttgtgt caatcgtcat tttaatggta aagacatgaa cgataatcaa
     1441 ttttcgggaa tgacgtccgc tgtatttaat atgggttgtt ataacatgag attttatcgg
     1501 aataaacaag gtcaatatgt gcagactacc atccataaac tcgcagtgaa taaacaattt
     1561 gaggagatgt gtcatcgact acctgacttt attcgtgcca gtggtaaggt attgaacgga
     1621 ttggttatcc gccgtgaaaa ggagaaagca ttatgcttaa ctggattagt ggcacgatag
     1681 ccattgtgat tttgggcttg tgtgtatggt tatggggtca gtcacagatg atagatagct
     1741 taagagctga gaaccgcgca caagcccaaa ccattgagca gcagcaaaaa gccaatcaac
     1801 ggctaaccga tagcttagag caagagcgac aagcggtcga aaaaatccaa aaaattgcaa
     1861 atgagctgcg gaataaagtg gagatggcca agaatgaaat tacatcaata ctggcacaag
     1921 acagttgtgc taaagctgat ttgcctaatg gcgttgctga tagcattaag cggctgcacc
     1981 agcagaacaa tcacaagcac tgagtatttg tatccgccgg cagcttacct tgtgccgtgt
     2041 gggcgaacag aatttagcgg tagaacctat ggcgatacgg ttgagtatct cgtcaaagtg
     2101 atgggagagc gtgatttgtg tgctaatcaa attgaccgta tcagagaatg gcaagcacaa
     2161 accaaacaag ggtttaaata gctaattatc taattaaccg ctattgaaaa tgggagcgta
     2221 acagctccct ttttgttttg atggaaagta aatattacat aatttagcta tggtttcggg
     2281 gttggttgga taaaacaccg aggaaatacc taagtgatgt gatttatata aaacaaaacc
     2341 ccgaacattt gcggtgttcg aggttttttt attacccatt gaaacggaat gagtaacaag
     2401 ttatggaaat tattacattt ttaaccgtaa ccatcaagga gattcttatg gaatatggtt
     2461 tatggcaaat aagcctagca gtaacagtcc ctattttggc gttcgtgtcg ccaaagctga
     2521 ttaatgctat tgctaatcta ttaaatgcaa tgaagtaaag gaatttaacc caatgactaa
     2581 aaaaggcgag gttaaagcca cgtctaaagg cgtgggtaaa ttaaccgata aacagaaacg
     2641 atttgtagaa gagtatctca ttgacttgaa tgcgactcaa gctgctatta gagctgggta
     2701 cagcgaaaaa acagcatact caatcggaga agagaacctg agaaaacctg aaatcagaag
     2761 tgcaattcag gaggctcaaa ataaacgctc ggagagaacc caaattacac aagatgatgt
     2821 gctaaatggg ttgcttgaag tgattgcaat gagtactggt aaaaaaattg tgacagaaac
     2881 tgatgttgca aaaaatgaca atggtgagtt ggtcggtttt gatattgcaa aaacaaaatt
     2941 cgaacctgcc gccgcaaata aagctcttga gttactcggt aaacatttag gaatgtttaa
     3001 agacaaggtt gatttaatta attctgatgg ttcactgaat cgaccgatgg tgattgaatt
     3061 aattgctccg agtttagacg atgaaagcac agattgaact accacctaaa ctgttgcctg
     3121 tttttgccaa agattatcgt tacaaaggtg cttatggtgg gcgaggttca ggcaagactc
     3181 gggcatttgc caaaatgtcg gcagtgtctg cttataaaag agcaatgcga ggggaaagtg
     3241 gcgttattct ttgcggtcgt gagtttatga actcattgga agaaagctct cttgaagaag
     3301 ttaaacaggc gatcaagtct gagccttttt tagcaaattt ttttgatgtc ggcgagaagt
     3361 atgttcgcac taaagatggg cggatttctt atgtgttttc cggacttcgg cataacttag
     3421 atagcatcaa atctaaagcc cgtattttga ttgcgtggat agatgaagca gaaacagtaa
     3481 gcgaaactgc ttggcggaaa ttaattccaa ccgtgcgtga gaataattcc gaaatatggg
     3541 taacttggaa cccggaaaat agagggagcg caacagatac tcgcttcagg caaaatccac
     3601 ctgaaaacag ctgcataatt gaaatgagtt accaagataa tccttggttt cctgatgtgc
     3661 tcgagcaaga gcggctaaat gacaaacagc ggcttgatga tgcgacttat cgctggattt
     3721 gggaaggtgc ttatttggaa gcgagcgaag cgcaaatctt tcgcggtaaa tatcaagaaa
     3781 tggaatttgt gccgaaccct gattttgatg gcccatatta cggcttagat tttggtttcg
     3841 cgcaagatcc gacggcggcg gtcaaatgct gggtatttaa tggcgatctg tacatcgagt
     3901 ttgaagcagg caaagtgggc ttagaacttg acgacaccgc agactttatt gccaaaggcg
     3961 tgccacaaat tgccgatcac gttgtgcgtg cagattcggc gcgtcctgag tcaattagtt
     4021 acctcaaacg tcacggctta ccgcgcatta tcggtgtgga gaagtggaaa ggctcggtgg
     4081 aagacggcat tgagcatatt aagtcctacg gcaaagtctt tattcaccca cgctgtcagc
     4141 aaacgctcaa cgaatttcgc ctttattcgt acaaaaccga ccgcttatcg ggcgatgtat
     4201 tgcctgtcgt gattgatgcc cataaccact ttattgatgc gctacgctac gcattaacgc
     4261 cgttgatgca agttaaaagt gccaaaggag ttttactatg agcctagagc aagacagact
     4321 ggctttttta gccgaagccc tagggcttgg caatatcaaa cgaagaacgt tatggaagga
     4381 gtttggctac cctaatacct taacattcaa ccatttctta aaagcctata agcgcaatag
     4441 tatcgctttt gcagcgatta accgcttact tgacggttgt tgggttgact gccccgtaat
     4501 cgttgagggt gagcaaaaga acgaatcaaa gcagacaacc gaatgggagt ccaaagttga
     4561 acgttttatg aaacgctact ggtcggcaat caaagaggca gatagacgaa accttgtcgg
     4621 taactattcg gcgttattac ttcaagtaag agatggtcaa caatgggatc agcctatttt
     4681 gcaaggcgcg ctatctagca ttggagagtt cggactggtt aaactgatcc ccgtgtggca
     4741 gtcacagctt tcagtgacag agtttcaaga ggatgtgacc gccgataatt acggcgaacc
     4801 gctgatgtat caattcagcg aatctgcctt tggcaaaaaa agcgtgtcac gaaatatcaa
     4861 ggttcacgca agtcgtgtaa ttttgctcaa tgagggtggc gactttaatt cccctcattc
     4921 tggagttcct ctgcttgagg cgggttacaa caagctagtt gatctcgaaa aaacatcggg
     4981 cggcagcgcc gaaggttttc tgaaaaacgc aagccgacaa ttagggatca aattaacaaa
     5041 agatgttgat ttgagacagc tcgaagacag cgcaaaagcc cttgggtttt cttcattttc
     5101 tgatgcgttg aacgacaaga tcaagaaaat caactcagga acagactcag ccttgattac
     5161 tcacgaaggt gatgcctctg tgttgtcagt cgcaccagca gatccgaagc caacgtggga
     5221 aatttctgca aatgagtttg ccgcctcagt acaaatccct ttcacgattt tgttcggaca
     5281 gcaaacaggg cgattggcaa gtgacgagga caaaaccgac tgggcaaacc gttgcaacgg
     5341 tcgtcgaaat ggttttctca ccgatgtgat aacacaacta cttaatcgct tgtggttcat
     5401 cggtgtgcta cctatgccaa aaaatagcga tgtcacagtg tcttggtctg acttacttgc
     5461 accaagcgaa aaagagaaaa tcgcaaacgc acaggctttg gcttcagttg caactacatc
     5521 gcagtctgcc tttggttttg ccgcggtaac agcgaacgaa atccgcgaat ctttagggtt
     5581 tgaacctctg cctgataatc tgattccgcc aacaattgac gacgaaaatg aagatcaaga
     5641 cgaaaatcaa gccactgcgg attccgacaa ataaagccga tccgctcaat atgggagtgt
     5701 cggttgccaa gctatttcgt cacattgatg tcatttactc acaaattaag cgctccatta
     5761 agcaacaaat cttgcaaaag atgtcactgc gactgcaagc aaataatttt gagtattttc
     5821 cttcgtatct tactgcgact gagttggcgg atttgcttga aaccttgcaa aagattatcg
     5881 acaaagagat tttgtcgaat ggttctcaag gcgaaaatct ctggtttgac tcctatatcg
     5941 acgaagcagc actaaaaggc actcagtctg cggtcactga tttaggtctg caatcagagg
     6001 cttatcgcag tcagcgaaac ttatcatcaa tcgttttcag tcctgcgtac ttcaaccgat
     6061 tggcgattgc acacatggca agttatagcg agtggcaagg tttaagcgac gagttgcgaa
     6121 aatctttggc gggggtgatc acggaggcgg ttttaaacgg caaaaacgtg aaagaaactg
     6181 cccgtgagat tcggcaaaaa cttgatgtgt catcaaaacg ggcgaagcga atagcgcaat
     6241 caagccagtt ggcggcatat cgtcgggcag aatggcaaga ggcggaagag gcaaaagaag
     6301 aacttgggct aaacaccaag ctcttacatt tttcagcgct caaagctact acgcgattag
     6361 cacacgccca acgccacggc aaatacttcg atgttgcaga ggtgcgcgaa tggtatagcc
     6421 gagacggcaa ccaattcaac tgttactgca agcagtctgt ggttgtggtc aacgccgacg
     6481 gtaaaacaga tattgagcct ctccttgttg ggttggctaa agaacgtaaa caatgggtag
     6541 gagcattaaa aaatgtcaaa aaataacgtg aacatcgtca ccgtcattaa cagcaaaaat
     6601 atcagcacag aatctattga cggagatcaa cacctgatta tccgcggcgt tgtgccgatt
     6661 gttgatgatg ttgtgatgaa cgacggactt tatcctgcgg atgaaatcaa taaaagctac
     6721 aacagcttag agggcaactt tatgccgctt gggcatccaa aaatcgaagg taaatacgtt
     6781 tcagcccaag atgttcgagc ggtgaacaaa catcacgtcg gtgcgtgggc gaagaacgta
     6841 cgcaaggaaa gtggcaaagt gctggtcgat atgtatatca accgccgctt tgcggaaggt
     6901 tcagaacagg gcaaaacctt gcttgaacga cttgacggaa tggtgacaaa caccaacgct
     6961 gaacctattc acgtttccac agggctgatt ctaaacaaag tcaagcaatc gggtaaatcc
     7021 aaaggcaaga aatatagctg gattgcgaca aatatgtcgt tcgatcatat tgcaatattg
     7081 cttgatgtgc cgggcgcggc gacacctgat gatggcgtgg ggatttttgt aaaccaagac
     7141 ggaagcgaaa tgggggtgga aaatgtaaac ctaacacaag cggcggatta ccgcacagaa
     7201 aagctgtttg acaaagtacg attttacctg atggcgaact cagatttatc gtttgaagaa
     7261 attcatcgcg cgctgcgtga agctattcgc cccgaaggca aagataagtg gtcattttgg
     7321 gtcgaagctg tttacccgaa atacttcatc tattcggacg ataacagcgg caagaaatac
     7381 aagcggagct attttattga cgaaaatgac aatgtgcagc tcactagcga acctgtcgaa
     7441 gttgtcaaga agattggcta cgaagaaatt acaaccaatg aggaacataa tccaatgaaa
     7501 accaaaatct tagccgtgtt gaatgcggca aacgtgaaaa cggaaggctt aaatgacgat
     7561 cagctcttgg aagcctacaa caagctgcaa gcggataagg gtaaagagcc tgaaaagcct
     7621 aaaaatgaag atgacagcga aattgatgag aaaatcaaga aagcagtcaa tgccgcgatt
     7681 gagccactcc agcaagcctt gcaagctaat actgacaaag agcaagcagc aatgcgtgcg
     7741 gcggtgaaag ccaagttcaa tatgagcgat gccgcagtca atgctttgca aggcgaggta
     7801 ttaactgagt tgtatgcgca aacgcaacaa tcggtcggat taaacaataa ttcggcaaac
     7861 ggcaacggcg acaatcaatg ggatggttac tcactcaacc aagaggagaa aggcaatgag
     7921 taatgtaatt tatcgcggtt cggtcaagcg tgaacctcaa acggtgaata tcgtgatcaa
     7981 cgatacttcg gcaccgggtg cagtagttaa actccacaac ggcaaattag aggcggcggc
     8041 tgatttaaaa ggtcgccgtt ttttattggg caacaaccgc tttaaggggc agacaatcga
     8101 acaagcgtat gcgaagggcg atactgcaac ggcgtttcgt ttagagcctg aacaggagta
     8161 ttacgcgcaa attgccgacg gcacttataa ctttggcgat gaactcacgg taaaacaagc
     8221 gggcggcaaa ctgaccaaag cggagacggg cgatgtagtg ctatttttct tcgacgaaga
     8281 atcgcagcgt gtaatcagtg gcggcaaagg ctatgccgat gtcgtggtgg caaacgccta
     8341 cactaaataa gaggtaacaa agcaatgttg aaattcacaa aagaacaaga acgcttcgtg
     8401 attaacgagc ggatcaatta cgacaaaaaa cacgcgctaa tggcagcaaa ttcggcgggg
     8461 ttattgggca acgcgatgac tttgccgaaa gatgtctggg cggaatggga tcgtgatgca
     8521 gtgcaaattc agcgcgaaga attggtggtg tttaacgatt tagcgggcat ttcaaaatca
     8581 atgccgattg gcaaacttat tcaccatttc caaacggtat ctgatagcgg ttcggttaat
     8641 atctcattag acggtcgttc atcagcgaaa accgataacg tggtgcttaa ctaccacggt
     8701 acaccgttgc cgattattga ttccactttc tcttttggct ggcgcgatat gtcggcagct
     8761 caaagcgaag ggtatcagat cgacggtgca gcacgcgcga acagtctccg caaggtggct
     8821 gaaaaaatgg aagatctcgc tttaaatggt gattcctcta ttgtggttgg caatgccaag
     8881 ctttacggct tgcgtactgc gccaaatcgt atgactaaca cacacaatct ggatttagcc
     8941 actgcgacac cgaaagaaat ttacgatgtg ttccgtgact tgattagcaa attccacgcg
     9001 aagaactact actctcctgt aacgctttat gtgaactatg gcgactattt cgcaatgagt
     9061 acacgtgatt actccgagca gaaatccgaa ggatctatct tgaacaaagt gatgacgatt
     9121 ccgcaaattg cgaaaatcgt gccagcaagc cgtgtaccgc aaaatgaaat tctcggcttg
     9181 tgcaagcgtt cggatgtgta ccaagtgctt aacggtatgc cgttagtgac tcgtccgatt
     9241 gcgcgccata atgaaaccga tgattactcg ttccaaatta tggcagccgt tgccgtggag
     9301 ttcaaattcg acgataaagg caatgctggc tatatccagt acactaaatc ctaaggcggt
     9361 gcaaaatgaa gtggctatta acacacgata gccacgagtt gaagaaaggc gatgtttacg
     9421 aaggcgaaac attaccgctt tggcttgtgg gcaaagctat cgaaatcgaa gaaaaatcct
     9481 ttgaagttgc aacgccttct gatcacgctg acggcgaaac tggcgaaact ggcgaaactg
     9541 gcgaaactgg cgaaactggc gaaactggcg aaactggcga aactggcgaa actggcgaaa
     9601 ctggcgaaac tggcgaaact ggtcagaaag gcaaaaaaga taaaggcaaa taactatggc
     9661 agcagaaata gagctatccg aagcgcgaac aacgcttgac gaactgggtt tcacttcgcc
     9721 gaactctctg cttgagcttt acgttcagca aacgaacacc ttagaccagg cgttgaacaa
     9781 atcggggtac agcgaagaaa cacagaaact gatcaagctc tatctggttg ctattcttgc
     9841 aatttcatct ggggcgcgcc gtataaagtc agaaggtgcg cctagcggtg caagccgaac
     9901 gtttgagtat gatccaaacg cgctagacaa ccttaagtcg gcgattcgct caattgatcc
     9961 gaaaggttgc gctgaaagtt tgctacccaa agataaagtt gtcggctttt tcgatgtggt
    10021 cggaggctgc taatgtcaaa tttatcgaat tggtcctaca ccgcaaaagc cacgctttgg
    10081 caggctaaag gaaagaacga tgacggcgtt ttagtttttt ctacgccaca aatcatttcc
    10141 tgtgactacg gtgcagatag aaagcgcgca cgttttgaga ttgggcgtga acaagcggtc
    10201 aaaaatgtga tctggacgga gttcgcccac gccaaattag gcgattatgt tctgattggc
    10261 gaaagcgagc aagccgatcc gattttggct ggcgcagagg aaattattca tatacagcgc
    10321 tttgctgata cgttcgaccg caatcgtgat gattttgcgc tgataacagg cgggtaaaat
    10381 ggggatcaaa gtaaaaggca taagacaggt caagcagcgg ctgaatcagg ttgttggcga
    10441 ggttcagagc caaaaagcaa ctcgggcaat gtttcgcatt ttgaatgcgg ttgcccctct
    10501 tgcggcgcac tatacgcctg ttgatacctc aacgttgatc aacagtcaat tcactgaaat
    10561 ggaagtgaac ggcacacgct tgattggaag agtgggttat tctgctaatt atgcggctta
    10621 cgttcacgat cctaatatca agcaaaactt ccgcaagcct tcagcgaaga aagagttttt
    10681 gacttccgcg ctgaacgaat caaaggctac gatcaaatcc attgttaaag aggagttgtc
    10741 actatgattt cttatgtcaa agccttcaaa caatggttag aggctcactc tcttgcagac
    10801 ggctatgttg tgcagcttta ccagtgggag gatacgcaaa aagccaagcc tgttattgtt
    10861 attcagccaa atagtggttc gccgcaagtt tctgacctga gcagcgaaca ctatctgcta
    10921 attagccttg ttgcaggtaa aaattcaggt tacacaatcg aagagcgagc aagggcgatt
    10981 atgtcaaaga ttttagttga gcctttcgct tcctttggtt acatcgaatc aatgggcgga
    11041 ttacccgccc caattttcac cgaagataat cgaatgattt ttcgcttacc acttcggatt
    11101 atttcaacaa atcaagagga gtagtttatg ggttctccat ctccacaaaa taacgcagaa
    11161 aatctcgtgg tggggcgtgc cgtagtttta gagtacggta cgactgatgc aaagccacaa
    11221 gaaagcgaat ggaaggttgc gggcgcaatg acgacaaaga gttgggattt tagcccaaat
    11281 tcggttacat cagaggctga tgatgctggc ggcttccctg aaaccttaat tacaaactca
    11341 gactttagta tttctggtga cggtgagtgg cgcaaacgac caaaatcgaa agaactaggc
    11401 atcaaagatc tagttgtgtt atacgcaaat gcggtaaaaa atcgcaccca accttacatt
    11461 tgggttcgtt tgaaatacgc aggcttaaca attctcggaa aaatggttat cactgcatta
    11521 agtagcgaag ccccaaccaa tgacttagtg aaattctcta tagagttcaa ggttggcgat
    11581 tcaagcacta tcgaaattgc tgcagcgtaa aaaatcccac cctctataac aagagcggtg
    11641 ggattatcat tatcctaggg atagttgtag gggctttcct aagctagcca aaatacgacc
    11701 aatagtgtcg atttttgtaa tatggcgagg ggctaaaatt cgctgtactt ctggcggacg
    11761 gatattagtt aatcgggcaa tttctgcctt gcttaccgct tgttcttgca ttgtattatg
    11821 caaaagcact tttgcgtaga tgctctctgg cataaacaca ggcacttcgt cggtatttac
    11881 tgggcgagtg agcggaaagc gtctatcttc atcgaaataa atttcaacac aagatagtag
    11941 cacgtcttct gccatttcca ttgcttcttc aaatgtatcg ccttgggtga tagcttctgg
    12001 caaatcagga aatgtcacta caaagccccc ttcttcagcg ggggtaaata acgctggata
    12061 aaacatattt tctccttatt caagggtgta gtgaaagccc cttaacatca ggggctttat
    12121 attattttaa gcctagttgc tttttaaccc cttccactaa gcctgttttt aactcttggc
    12181 ttgggtgttt ggggaggtgg cttcttttgc ccttgtagta gagtttcaaa tgctttgatc
    12241 cattttcaac ctctacccca ttagccttta gccaccttag gaattcgctt tgcttcacta
    12301 cctcctccta tttgtttaac ttgggttcat tatatgcaaa aatgctaact ttgtcaacag
    12361 aaagttagca aaaatgctaa tttttttgtt gaggtgaaaa tggttagcta tccaattact
    12421 gaaattggtg aaatgcttat tgcaaccgat gggcgagact ttttattcag accgagtttc
    12481 aaaaatatct acaaaattgg aagcccacaa gaaattgtaa aaatctatgc actattgcac
    12541 ggctgtgaga ttcgagagat attgccgcac attttaacca aatcggagtc tcatcaatct
    12601 tacttttggt cagtgctgaa ttcgcctgtt tttggcagaa aaattctgca aaacgcaatg
    12661 atcgtgatgt cgtgctgttg cgatgatgat atttccctgt tgattggcga gtggttgccg
    12721 tcaaagtctg gcgttaggta caagttgggt aaaatgccac ctgagacaat tatcaatttt
    12781 gcaaaaaatt taatgggtca cggagtgatt ggttgctgcg aattgccaaa gaaagaacaa
    12841 tctgcaaaaa atgaattttc agaaagtttt gatgctgttg agtacataag tttagcccga
    12901 actcatttca atatgagccg cgaagacgct gaaaatctca cgatgaccga attgcagcaa
    12961 cttctaaaaa gtcaaatgcc tgccgaaaat aatagctatg gacagttcac cgaagaagag
    13021 tacgacagaa ttatggcgga atatgagaaa agaagagcgg cttaaatagc cgctaacatt
    13081 taaaagaacc tacctgatag aacgggagtg aaatccttgt aatgtgctga ataaaaaagc
    13141 tctcttatgt cattgagtag ctcaaattca tctttggatc ttgcgttgca gattgccaca
    13201 tagtcatcta cgcaggataa aatacgttca atactgactt cttttatcca gcatacttct
    13261 tggttttcat tcagccagtt ggcaaagtct tgtttagtgc agtctttttt cataagttct
    13321 cctttttgat tatttgcaaa aaaaatcata tcaaatcatc cgttgttatc tattggcgaa
    13381 aataattttt gcgacacaga tcgcaaaaat aggttttgac ggtggaaaaa cgaatcttaa
    13441 ttcgtagaat ggtaaaattt ctttattttt aaggtatttt tatgaaaaaa atgattgttt
    13501 ttgtagcttc tgttttgtca tcttttgtta ttgcatcgga ttgggaaata atagaagcgg
    13561 ctcaaaataa ggtgaaagca gagcttataa gaaaatcaga aaaaaaagca gagttatgtt
    13621 taaatgttgc tcaggctgtc ttaattacta atcaaaaagt acaaaaagat actttctcaa
    13681 tgtgtggtga attttcgaca gagattgtct tttctgactc taaagcatat agccttgcag
    13741 tatgtggaaa ggttaaaggt aaagatatta tcggaaatcc gatagagagc gactatatat
    13801 accaaagagc aactaaggat ttttacttta attataatgg caaaaaatca gcttattatg
    13861 atcctcaatc tgatcttgca aggcttcaag aggtctcaga ctctatgttt aatgctatgc
    13921 aaaatagat
//
LOCUS       DANLZC010000059        13809 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.059, whole genome shotgun sequence.
ACCESSION   DANLZC010000059 DANLZC010000000
VERSION     DANLZC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.059"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            126..2402
                     /gene="gshAB"
                     /locus_tag="PXE44_001355"
     CDS             126..2402
                     /gene="gshAB"
                     /locus_tag="PXE44_001355"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL5588867.1"
                     /translation="MKLQKLIRENHLALLFQQGNFGLEKESQRVDRNGNIVTTPHPAV
                     FGNRSYHPYIQTDFAESQLELITPPNAKLEDSFRWLSAIHEVVLRSLPEEEYIFPLSM
                     PAGLPPEEQIQEAQFDNPEDVQYRQHLSKNYGKYKQMVSGVHYNFQLSTEFVEKAFAL
                     QTEYSELKAFQNALYMKLANNFLRYQWILLYLLAATPTVESQYFYEKSPLARGQLVRS
                     LRSSPYGYVNSEKVVVNHDSLESYVESLEANVANGLLIAEKEFYSNVRLRGGKKAREL
                     LENGVKYAEFRLFDLNPFAPYGIELNDAKFIHYFLLGMLWLEETSGQKEVEIGKQKLY
                     QVALEDPRTETAFRAEGEAILNQLLAMLEELSAGQESLDLVREKLAQFADPSKTLNGR
                     LIAAIEEVGSYKALGAKLAQTYKAQAFERFYALSAFDNMELSTQALFFDLIQQGIEVE
                     VLDENDQFLALKFGDHLEYVKNGNMTSRDQYISPLIMENKVVTKKVLAKAGFNVPKSV
                     EFTNLEQAVAHYPLFEGKAVVIKPKSTNYGLGITIFQQAVKNREDFAKAVEIAFREDK
                     EVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVVGDGIHSVKELVERKNDDPLRGDGS
                     RSPLKKIALGEIEQLQLKEQGLTVDSVPAKGQTVQLRANSNISTGGDSIDMTDQMHES
                     YRQIAVGIAQAMGAKVCGVDLIIPDLTKPAEPNLSSWGVIEANFNPMMMMHIFPYQGK
                     SRRLTKAVIKMLFPEIGN"
     gene            2724..3701
                     /gene="manX"
                     /locus_tag="PXE44_001356"
     CDS             2724..3701
                     /gene="manX"
                     /locus_tag="PXE44_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL5588868.1"
                     /translation="MSIAIIIATHGVAAEQLLKTTEMLIGEQEDVAYIDFVPGENAET
                     IMGKYQALVEGPLSHCDQVLFLVDMWGGSPFNAANRFQDGKSGMDVVAGVNIPMLVNT
                     CMARDDVESLEELVEVALEAGSTGIRSLNHQPEVAEVQTATPAQETAAVQQPEIVDDG
                     RRMTIALARIDDRLIHGQVATVWTKESKVNRIIVVNEGVAKDSVRTTMLKSVAPLGVT
                     AHVVDVPKMIRVYNNPEYADDRVMLLFTNPSDVLALIEGGVPLNSINVGGISHKEGKQ
                     MVSHSVAVDQNDIDAFKAIDAKGVELEVRKVSSDSRQYMMDLLKKHNLI"
     gene            3713..4513
                     /locus_tag="PXE44_001357"
     CDS             3713..4513
                     /locus_tag="PXE44_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005716709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL5588869.1"
                     /translation="MELSALQIVLLFLVACISGMGSILDEWQTHRPLIACTMIGLVLG
                     DVTTGVIVGGSLELLALGWMNIGAAVAPDAALASIVSTILVIVGRQDISTGIAIAIPL
                     AAAGQVLTYVVRAITVGFQHAADKSIEDGNLSRLDWLHRGALLLQAMRIAIPALIVAL
                     TAGTDAVQTMLNAIPEVVTTGLKIAGGFIAVVGYAMVINMMRAGHLMPFFFAGFVIAG
                     FTDFNLVALGVLGTVIAMVYIHMHPKYNKSQQVVVAAAPKNDLDNRLD"
     gene            4527..5363
                     /locus_tag="PXE44_001358"
     CDS             4527..5363
                     /locus_tag="PXE44_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDL5588870.1"
                     /translation="MTTEIKKVTKSDLNAVVHRSNLFQGSWNFERMQALGFAYAMVPV
                     IKRLYPDPNGPGRKEAIKRHSEFFNTQPFVGAPILGVTIAMEEERANGKEIDNAAING
                     IKVGLMGPLAGVGDPIFWGTVRPVFAALGAGLALKGELLGPILFFALFNIVRLAARYY
                     GVVYGYKKGLDVVQDMSGGLLQKLTEGASILGLFIMGALVQKWTSINVPLVLSTIQKQ
                     DGTIEVTTVQSILDSLMPGLLPLLLTFACMWLLRNRVNAIWIILGFFVIGILGAATGA
                     LA"
     gene            5456..5941
                     /locus_tag="PXE44_001359"
     CDS             5456..5941
                     /locus_tag="PXE44_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDL5588871.1"
                     /translation="MTNTILILFILLGLVFAIYDQVFMHKLKGTTLLEIRLKKQKTID
                     TWLLIGLIVLSISYGVQNQIQPFTLYLLATCIILSVYLAFFRSPRLLLKQHGFFFANV
                     FFNYDKIYQVNIAEGKADEKILVIDLHSGRRLLAYVENAEDLQPVVDFFGGYKKESEQ
                     K"
     gene            complement(6027..6818)
                     /gene="tpiA"
                     /locus_tag="PXE44_001360"
     CDS             complement(6027..6818)
                     /gene="tpiA"
                     /locus_tag="PXE44_001360"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL5588872.1"
                     /translation="MARRPLVMGNWKLNGDKAFTKTLIADLKAELHGVEGCDVAIAPP
                     VMYLAHAEASLAGCGCGCSDKGLIALGSQNVDVNVKGAFTGDISPEMLKEFGAKYIII
                     GHSERRTYHKESDEFIAQKFKVLKDAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
                     NAHGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHKFIRDHIAAKSQAVADQVIIQY
                     GGSVNDANAAELFTQPDIDGALVGGVSLKAPAFAVIVKAAAAAKA"
     gene            6978..7997
                     /gene="truD"
                     /locus_tag="PXE44_001361"
     CDS             6978..7997
                     /gene="truD"
                     /locus_tag="PXE44_001361"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDL5588873.1"
                     /translation="MKLNYLYSPPQAVGRLKAEYADFIVREELGYELAGEGEFVAVKI
                     RKTNASTLFVGEKLAKFVGISEREMSYAGLKDRHTVTEQWFCLHLAGKETPDFSAFEC
                     EGVEVLEVTRHNRKIRVGSLAGNHFELLLRDVAESEELIYRLQQLQAVGFPNYFTEQR
                     FGRDGHNLTQALRWANGEISVKDRKKRSFYLSAARSEVFNLVVSQRINDCLTQKILEN
                     DYVQLAGSNSFFCVKSDEIAETQARLAQQDLLLTAPLIGERSLELECNPAEKAIIEQH
                     AQLVALMQKERMAAARRAMLCKPQNLEWQFEQEGLRLKFFLDSGSYATALVRELILLT
                     EESEA"
     gene            8168..8956
                     /gene="surE"
                     /locus_tag="PXE44_001362"
     CDS             8168..8956
                     /gene="surE"
                     /locus_tag="PXE44_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDL5588874.1"
                     /translation="MRILISNDDGFHAVGIQTLAKALRDAGHIVTIIAPDRNRSAASS
                     CLTLVDPLRVHRFDEYNYAVIAGTPADCVHLALNGLFDGKFAEKFDLVVSGINHGANL
                     GDDVVYSGTVAAALEGRHLPLPSIAVSLVGRKHSSFLDGENHFGTAAQVVLDLLEKME
                     TQRFPSNHVLNVNVPNLPYSELKGTMITRLGERSPAAEIVKQKDPRNADVYWIGVTGE
                     PLDESFGTDFYALNRDCVSITPIQVDMTAHKTIATLKGIFNETV"
     gene            8943..9527
                     /locus_tag="PXE44_001363"
     CDS             8943..9527
                     /locus_tag="PXE44_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5588875.1"
                     /translation="MKLFETIYDKTMQWSKHRFAAFWLSFVSFIEAIFFPIPPDVMLI
                     PMSMAKPQNAFKYAVYTTLASVAGGVIGYFIGLYATDWVQGVIANWGMQANFDKAKLW
                     FETWGVAVVFLAGFSPIPYKVFTICAGVMQMAFFPFVITAAISRFARFILVAKLSAWG
                     GKKYEQKIRRSIELIGWGTIVLAVVAYIIYTLMK"
     gene            9545..10045
                     /locus_tag="PXE44_001364"
     CDS             9545..10045
                     /locus_tag="PXE44_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL5588876.1"
                     /translation="MKKSLFVLSLISFALVGCSSNSGESTSAVETTEANATWQSADSI
                     QTAPMPASMNQPIATQPTYQQPTYSAPQPISAAPAPMPVSSGSDSSQVETVGNCQVVR
                     DANNAPIYSQITKGCYTESHYTVGKSDTLYLIGYLTGTNANQIAQLNGLNTGSPLKVG
                     QVLRVR"
     gene            10058..10258
                     /locus_tag="PXE44_001365"
     CDS             10058..10258
                     /locus_tag="PXE44_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588877.1"
                     /translation="MNRTLLAFAISCAFLTACSSPTQPQVEDPNMLPAGTLQPIEGSG
                     ASQDSYSWPSDIQSTSMPDSMK"
     gene            10277..11557
                     /locus_tag="PXE44_001366"
     CDS             10277..11557
                     /locus_tag="PXE44_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL5588878.1"
                     /translation="MKKQFILLPLVSAVLAACSSNNPAPVVNASGNNELSPGVMQPVD
                     VSAGAINSSGGWQSDIQSAPMPSSMNTTPVQTMPQPVSVPQPVATVPQPITNPVITEQ
                     PPQPTTTTKIVKKTKTVEKKVDQNFEIPRDANNAPMYNQIQKGFYDGSTYTVRKGDTM
                     FLIAYIVGKDVKEIAALNNMSEPYQLSVGQKLKTGKSATETITVEEKVTVPVEPQITY
                     QQGANGTTYASGGNITGPVKAGTANADVTPVNNGIKATTATAPAVTASVGTVNATEDR
                     TASTIRAIDGTASGAVQSSNTVSAPASSIKWQWPTSGRVISGFSSAEGGNKGIDIAGN
                     KGQDVRAAAAGKVVYAGNALQGYGNLIIIKHTDDFLSAYAHNNTIEVDEQETVKAGQK
                     IATLGSTGTNTNKLHFEIRYKGKSVDPARYLPRK"
     gene            11798..11989
                     /locus_tag="PXE44_001367"
     CDS             11798..11989
                     /locus_tag="PXE44_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588879.1"
                     /translation="MDPTEPSHRIYILLSEDRFQAEVFSTKLSESSMLLEAVKGGYIS
                     KDGKVRLLKKAEGWKIYYE"
     gene            11982..12548
                     /locus_tag="PXE44_001368"
     CDS             11982..12548
                     /locus_tag="PXE44_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL5588880.1"
                     /translation="MSETKIRCDWCKSSEIYMKYHDEEWGKPEFDSLKLFEKICLEGQ
                     QAGLSWITVLKKREAYRQAFHQFNPEKIAKMGEEEIDLLMQNTSLIHHRAKLEAIIKN
                     AKAYITMQQNGEDFSRFIWSFVNYQPQINDVPTLEAVPAHTETSKAMSKALKKRGFVF
                     VGETTCYAFMQSMGLVNDHLNNCVFKHK"
     gene            complement(12595..12825)
                     /gene="acpP"
                     /locus_tag="PXE44_001369"
     CDS             complement(12595..12825)
                     /gene="acpP"
                     /locus_tag="PXE44_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL5588881.1"
                     /translation="MSIEERVKKIIVDQLGAKAEDVKPEASFIEDLGADSLDTVELVM
                     ALEEEFDIEIPDEEAEKITTVQSAIDYVQNNQ"
     gene            complement(13012..13689)
                     /gene="rpe"
                     /locus_tag="PXE44_001370"
     CDS             complement(13012..13689)
                     /gene="rpe"
                     /locus_tag="PXE44_001370"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL5588882.1"
                     /translation="MSKNPYLIAPSILSADLARLGDDVAEVLKAGADLIHFDVMDNHY
                     VPNLTFGPAICKALRDYGIQCPIDVHLMVKPVERLIPDFAKAGADYITFHPEATEHID
                     RNLQLIRDLGCKSGLVFNPATPLSYLDYVMDKVDVVLLMSVNPGFGGQSFIPSTLDKL
                     KEVRRRIDESGYNIRLEVDGGVKINNIADIAAAGADMFVAGSAIFDQPDYQAVIAQMR
                     DELRKVG"
BASE COUNT         4099 a         2846 c         2904 g         3960 t
ORIGIN      
        1 gaagcagtat ttctgttata accaaatcat ctacttcatt ccaataaact aaaacccact
       61 atttcactaa gtctttcatt tgtgttattg tgccgataca tttttttgac tacaaaggat
      121 agatgatgaa actacaaaaa cttatccgag aaaatcattt agcattgctg tttcaacaag
      181 ggaattttgg gcttgagaaa gagagccaac gggttgatcg caacggcaat atcgttacta
      241 ctccccaccc tgccgtattc ggtaatcgct cttaccaccc ttatattcaa accgattttg
      301 ccgaaagtca gctagagtta atcactcccc cgaatgctaa attagaagac agttttcgtt
      361 ggctctctgc tattcacgaa gtggtattgc gttcgttgcc agaggaggaa tatatcttcc
      421 ctctcagtat gccggcaggc ttgccgccgg aagagcaaat tcaagaagca caatttgaca
      481 accctgaaga tgtgcaatat cgccaacacc tttccaaaaa ctatgggaaa tataagcaaa
      541 tggtaagcgg cgtgcattat aattttcaac tttcaacgga atttgtcgaa aaagcctttg
      601 ctttacaaac ggaatatagc gagctgaaag ccttccaaaa cgcactttat atgaagttag
      661 ctaataactt tttgcgttat caatggattt tgctctattt attggctgcc acgcctacgg
      721 tggaatcgca atatttttat gaaaaatcac cgcttgcgag agggcaactt gtccgcagct
      781 tgcgttcaag cccttatggt tatgtgaatt cggaaaaagt ggtggttaat cacgacagct
      841 tagaaagcta tgttgaaagc cttgaagcca atgttgccaa cggattatta attgccgaaa
      901 aagaattcta ttccaatgtg cgtttacgag ggggcaaaaa agcccgtgag ctattggaaa
      961 acggtgtgaa atacgccgaa ttccgtttat ttgatttaaa ccctttcgcc ccttacggca
     1021 ttgagctgaa tgatgccaaa tttatccatt atttcttact cggtatgctt tggctggagg
     1081 aaacaagcgg tcaaaaagag gtcgaaatcg gcaaacagaa actttaccaa gtcgcacttg
     1141 aagatccaag aacagaaacc gctttccgtg ccgaaggtga agccatttta aatcaacttt
     1201 tagcgatgct tgaagaatta tctgccggtc aggaaagcct agatttggtg cgggaaaaat
     1261 tagctcaatt tgctgatcca agcaaaaccc tgaacggacg tttaattgcc gcaatagagg
     1321 aagtcggcag ttataaagca ttgggggcta aattggctca aacctataag gcacaagcct
     1381 ttgagcgttt ttatgcgtta tcggcatttg ataatatgga actttccact caagccctgt
     1441 tttttgattt gatccaacaa gggattgagg tggaagtact ggacgaaaac gatcaattcc
     1501 tcgccctaaa atttggcgat catttggaat atgtgaaaaa cggcaatatg accagccgag
     1561 atcaatacat ttcaccgctg attatggaaa acaaagtggt gacgaaaaaa gtattggcaa
     1621 aagccggctt taacgtgccg aaaagcgttg aatttaccaa ccttgaacag gctgtggcac
     1681 attatccgct atttgaaggc aaagcggtgg tgattaagcc gaaatcgact aactatggtt
     1741 tagggattac catcttccaa caagcggtca aaaatcgaga agattttgca aaagcggttg
     1801 aaattgcgtt ccgtgaagac aaagaggtga tggtggaaga ctatttagtc ggtactgaat
     1861 accgcttctt tgtactgggc gatgaaaccc ttgcggtgtt actgcgtgta ccggcgaatg
     1921 tagtgggaga tggcattcac agtgtaaaag agctggtgga gcgtaaaaat gacgatccgc
     1981 tacgtggcga tggctctcgt agcccgttga aaaaaattgc cttaggtgaa attgagcagt
     2041 tacaacttaa agagcaaggc ttaaccgtag attccgtacc ggcgaaaggt caaacagttc
     2101 aactgcgtgc caattccaac atcagcaccg gcggcgattc gattgatatg accgaccaaa
     2161 tgcacgaaag ctatagacaa attgcagtgg gaattgctca ggcaatgggg gctaaagtgt
     2221 gtggggtgga tttgatcatt cctgatctca ccaaacctgc cgagccaaat ttaagctctt
     2281 ggggcgtcat tgaggcaaac ttcaacccaa tgatgatgat gcacattttc ccttaccaag
     2341 gcaaatcccg ccgtttaacc aaagcggtaa tcaaaatgct gtttcctgag ataggcaatt
     2401 aaattttctc tctatacctc ttttaaggag aatttactca agaattctcc ttaatttatt
     2461 tctaaagttt tactccaatt aaatttcact ttgtgcaaaa cactccaaaa gaagaatttt
     2521 tttgtataag taaaaacgat cgaaaatatc tccgttaaca aaatgttata aagaaaagac
     2581 atttagccca atttatagcc aaaaaagctt attgtctgtg atatagatca cacttttgta
     2641 attaatacat tgtgtgaaat aattatcata ttaaaatctc gccccagtaa ataaacaact
     2701 taaaaaaccg caaggagtaa caaatgagca ttgctattat catagcaacc catggtgttg
     2761 ctgcagagca acttctcaaa acaactgaga tgttgattgg tgaacaagaa gatgttgctt
     2821 acattgactt tgttcccggc gaaaatgcag aaacgattat gggcaaatat caagcattgg
     2881 ttgaaggtcc actatcacac tgtgatcaag tgctgttttt ggtggatatg tggggaggaa
     2941 gcccattcaa cgccgcaaac cgattccaag atggaaaatc gggtatggat gttgttgcgg
     3001 gtgtgaatat tcctatgtta gtgaacacct gtatggctcg tgatgatgtg gagtcgttgg
     3061 aagaattggt ggaagtcgca ttagaagccg gctcaaccgg gattcgttca cttaatcacc
     3121 aacctgaagt ggctgaggtg caaaccgcta ctcctgcaca agaaactgca gctgttcaac
     3181 agcctgaaat tgttgatgac ggacgtcgta tgaccattgc ccttgcccgt attgatgacc
     3241 gcttgattca cggtcaagtt gcgacggttt ggacaaaaga aagtaaagtc aatcgcatta
     3301 ttgtggtaaa cgaaggtgta gcaaaagata gtgtacgaac tactatgcta aaaagtgttg
     3361 ctcctcttgg cgtaacggct cacgttgttg atgtacctaa aatgattcgt gtctataaca
     3421 accctgaata tgccgatgat cgtgtaatgt tactctttac caacccaagt gatgtcttag
     3481 ccttaattga aggcggtgta ccactcaata gcattaacgt gggcggtatt tcgcataaag
     3541 aagggaaaca aatggttagc cattctgtgg cggttgatca aaatgatatt gatgccttca
     3601 aagcaattga tgcgaaaggc gttgaattag aagtacgcaa agtctcaagc gatagccgtc
     3661 agtatatgat ggatttattg aaaaaacata acttaatcta aaggaaatta ctatggaact
     3721 ttcagcatta caaattgtgc ttttattcct cgttgcctgt atctcaggta tgggctcaat
     3781 tcttgatgaa tggcaaaccc accgtccgtt aattgcctgt acgatgatag gcttagtgct
     3841 tggtgatgtg actaccggcg ttattgttgg tggctcatta gagttattag ctttaggttg
     3901 gatgaacatc ggtgccgctg ttgcaccaga tgcggcatta gcctctattg tctcaaccat
     3961 tttagtcatt gtaggtcgtc aagatatttc aactggtatc gccatcgcta ttccgcttgc
     4021 agctgccggt caagtattaa cctatgttgt gcgtgcaatc accgtaggtt tccaacacgc
     4081 agcagataaa tcaattgaag acggtaactt aagccgttta gactggttac atcgtggtgc
     4141 tttattgctt caagcaatgc gtattgccat tccggcatta attgtggcat taacagcagg
     4201 tacagatgcg gttcaaacaa tgttaaatgc cattcctgaa gtggttacaa ccgggctgaa
     4261 aattgccggt ggcttcatcg ccgtggtggg ttatgcgatg gtgatcaata tgatgcgtgc
     4321 aggtcattta atgccgttct tcttcgcagg ctttgtaatt gcaggcttta ccgatttcaa
     4381 tcttgttgcc cttggggtat taggtacggt tattgcaatg gtttatatcc atatgcaccc
     4441 taaatataac aaaagccaac aggtagtggt ggcagctgca ccaaaaaatg atttagacaa
     4501 ccgtttagac taagagggca tttaaaatga caactgaaat caaaaaagta acaaaaagcg
     4561 atttgaatgc cgttgtacat cgttcaaact tattccaagg ttcgtggaac tttgagcgta
     4621 tgcaggctct tggttttgcg tatgcgatgg taccggttat caaacgttta tatccggatc
     4681 caaatggccc gggtcgcaaa gaagcaatta aacgccattc tgagttcttt aacacccagc
     4741 cattcgtcgg tgcaccgatt ttaggggtaa ccattgctat ggaagaagaa cgtgccaacg
     4801 gtaaagaaat cgacaatgcc gcaattaacg gtattaaagt tggtttaatg ggaccattag
     4861 ccggtgtggg tgacccaatt ttctggggaa ctgttcgccc tgtatttgcc gcattaggag
     4921 caggcttagc cttaaaaggt gagcttttag gcccaatcct gtttttcgcc ttatttaaca
     4981 tagtgcgttt agccgcacgc tattatggcg tagtgtatgg ctataaaaaa gggttagatg
     5041 ttgtacaaga tatgagcggc ggtttattgc aaaaattaac cgaaggtgct tcaatcttag
     5101 gtctgtttat tatgggggca ttggtacaaa aatggacgag cattaacgtt ccgcttgtgc
     5161 tttcaaccat tcaaaaacaa gacggtacca tcgaagttac cacagtgcaa agcattctcg
     5221 acagcttaat gccgggccta ctcccattat tattaacctt tgcttgtatg tggttacttc
     5281 gtaatcgagt aaatgctatc tggattatcc tcggtttctt tgttatcggt atcctcggcg
     5341 ctgcaaccgg tgcattagct taattgcaaa attttctgtg aaaacgaccg cttgaacttg
     5401 aacattcaag cggtttttta tacgctttag cccaaataaa ttcacttgct taattatgac
     5461 caataccatc cttattctct ttattttact tggcttagta ttcgccattt atgaccaagt
     5521 ttttatgcac aaactcaaag gcacaacctt actggaaatc cgtttaaaaa agcaaaaaac
     5581 aattgatact tggttattga ttggtttaat cgtcttgtca attagctatg gcgtacaaaa
     5641 tcaaatccag ccttttacac tttacctgct ggcgacttgc attattctgt cggtctatct
     5701 cgcctttttc cgttcccctc gcttgttgtt aaaacagcac ggttttttct ttgcgaatgt
     5761 cttttttaat tacgacaaga tttatcaagt caatattgca gaaggaaaag cagacgaaaa
     5821 aattttggta attgatttac atagcggaag aaggctgctt gcctatgttg aaaatgcgga
     5881 agatcttcaa ccggtagtgg atttttttgg cggttataaa aaggaaagtg agcagaaata
     5941 gtttgcgaac aaaacacaag cggtcatttt tccctgaaat tttgcaaaaa actgaggaaa
     6001 aatgaccgct tgtaaaatcg gttaaattag gctttagcag ccgctgccgc tttaacgatc
     6061 accgcaaaag caggcgcttt aagtgaaaca ccgcctacta atgcaccgtc aatatccggt
     6121 tgagtgaata actctgctgc attggcatcg tttacagagc caccgtattg aatgatcact
     6181 tggtcagcca ctgcttgcga tttagctgca atatggtcac gaataaattt atgcactgct
     6241 tgtgcttgtg ccggagtcgc tgatttgccg gtaccgattg cccaaattgg ttcgtaagca
     6301 ataactgcac cgttgaatgc ttccacaccg tgcgcgttga tgaccgcatc aatttgtctt
     6361 gcacacactt cttcagtttt acctgcttca ttttcggctt cagattcacc gatacataat
     6421 acaggcacta agccggcatc ttttaatact ttgaattttt gagcaataaa ttcatcgctt
     6481 tctttgtggt aagtacggcg ttctgaatgc ccgataataa tatattttgc cccaaactct
     6541 tttaacattt caggtgaaat atcaccggtg aatgcccctt ttacgttcac atcaacgttt
     6601 tgtgaaccaa gtgcgattaa gcctttatca ctgcaaccac agccacaacc ggcaagtgat
     6661 gcttcagcgt gtgccaaata catcaccggt ggagcgattg ctacatcaca gccttcaacg
     6721 ccatgtaatt ctgcttttaa atctgcaatt aaggttttag taaacgcttt atcaccgttt
     6781 aatttccagt tacccattac aagtggacga cgtgccattt tgtttctcct atatgaagtt
     6841 gaacttaaat ataaacgttg caaactatac caaattttaa tgtcgttttg ttgtttttat
     6901 tttgcttttt ctgatctaga tcgaaaggtt taacgtataa tgccgaaatc ttatttttta
     6961 tgttattcga gaagtgaatg aaactgaatt atttatactc tcctcctcaa gctgttgggc
     7021 gactaaaagc cgaatatgca gactttattg tgcgtgaaga actgggctac gaactagccg
     7081 gcgaagggga atttgtggcg gttaaaatcc gcaaaaccaa cgccagcacg ctgtttgtgg
     7141 gggaaaagtt agccaaattt gtcggtattt ccgagcgtga aatgagttat gcaggcttaa
     7201 aggaccgcca caccgtgact gagcaatggt tctgcttaca cttggcaggc aaagaaacgc
     7261 ccgatttctc cgcctttgaa tgcgaaggcg tggaagtgct ggaagtgacc cgccacaacc
     7321 gcaaaattcg tgttggttcg ctcgccggca atcattttga attattgcta cgtgatgtag
     7381 cagaaagcga agagttgatt taccgtttgc aacagttgca agcggtcgga tttcccaatt
     7441 attttaccga acaacgtttc gggcgagacg gacataacct gactcaagcc ttgcgttggg
     7501 caaacgggga aattagcgtg aaagatcgca aaaaacgcag tttctacctt tccgctgccc
     7561 gctcggaagt gtttaattta gtggtttcac agcggattaa tgactgctta acccaaaaaa
     7621 tcttggagaa tgattacgtt caacttgccg gctctaacag ctttttctgc gtgaaatcgg
     7681 acgaaattgc tgaaactcaa gcccgtttag cacagcaaga tctacttctc accgctccgc
     7741 tgataggcga aagatcgctt gaattagaat gcaatcctgc tgaaaaagcc atcatagagc
     7801 aacacgctca attggttgcc ttaatgcaaa aagagcgtat ggcagccgct cggcgagcaa
     7861 tgctttgtaa gccacaaaat ctggaatggc aatttgaaca agaagggcta cggttaaaat
     7921 tctttttaga ttcaggcagt tatgccactg cactggtgcg agagttaatt ctgcttaccg
     7981 aagagagcga agcttagtcc ataacacata acaagcggta agattttaat cattttttgc
     8041 aatttgcaaa actttaacaa aatcttaccg ctttttgcac ttttagacta aaaaaactgt
     8101 cgaaaaattg ttagaattgc gatctttgta tttagagtct atcaagatgt aagatctaaa
     8161 ggaaattatg cgaattttaa ttagtaatga cgatggtttt cacgctgtcg gcattcaaac
     8221 tttagccaaa gccttgcgag atgcaggcca tatcgtcacc attattgcac cggatcgtaa
     8281 ccgcagtgca gcttcaagct gcctaacctt agtagatcca cttcgggtgc atcgttttga
     8341 tgaatataat tatgcggtga tcgccggcac acctgccgat tgcgtgcatt tagccttaaa
     8401 cggcttgttt gacggcaaat ttgcagaaaa atttgatttg gtggtctccg gcattaatca
     8461 cggggcaaat ttaggcgatg atgtggttta ctccggtacg gtagcggcag cattggaagg
     8521 ccgccacttg ccattaccaa gtattgcagt atcactggtt gggcgtaaac actcttcgtt
     8581 tttagacggt gaaaatcatt ttggaacggc agcacaggta gtgttagatt tattggaaaa
     8641 aatggaaacg caacgttttc catcaaacca cgttctcaat gttaatgtgc cgaatttacc
     8701 ttattcagag ctaaaaggca caatgatcac ccgcttaggt gaacgttcac ccgctgcgga
     8761 aattgtgaag caaaaagatc ctcgtaatgc tgatgtctat tggattggtg taacaggtga
     8821 accactggat gaaagttttg gtactgattt ttatgcatta aaccgagatt gcgtttctat
     8881 cacaccaatt caagtggata tgaccgctca taaaacgata gcaaccttaa aaggcatttt
     8941 taatgaaact gtttgaaaca atttatgaca aaacaatgca gtggtctaag caccgcttcg
     9001 ctgccttttg gttgagtttt gtcagtttta tcgaggcgat ctttttcccg attccgccgg
     9061 atgtgatgtt gattccgatg tcgatggcaa aaccgcaaaa tgcgtttaaa tatgcagttt
     9121 acactaccct tgcttcggtt gccggtggtg ttattggtta ttttattggc ttatatgcta
     9181 ccgattgggt gcaaggcgtc atcgcaaatt ggggaatgca agcaaacttt gataaagcaa
     9241 aattgtggtt tgaaacttgg ggtgtcgcgg tagtattttt agccggtttt tcgccgattc
     9301 cctataaggt atttaccatc tgtgccggtg taatgcagat ggcgttcttc ccgtttgtga
     9361 ttactgctgc gatttctcgc tttgcccgtt ttattttggt agcaaaactc tctgcttggg
     9421 gtggcaaaaa atatgaacaa aaaattcgcc gctcaattga attaattggt tggggaacaa
     9481 tcgttttagc cgttgttgcc tatataattt atacattaat gaaataaatt ttaaggatat
     9541 aaaaatgaaa aaatcacttt ttgttttatc tctgatttct tttgctttag tgggttgttc
     9601 aagcaattcg ggtgaatcca cttctgcggt agaaacaaca gaagcaaatg caacttggca
     9661 atcagcagat agcattcaaa ctgcaccaat gcctgcctcg atgaatcaac ctattgccac
     9721 tcagccaacc tatcagcagc cgacttactc tgcaccacaa ccaatttcgg cagcaccagc
     9781 accaatgcct gtttcaagtg gtagtgatag ctctcaagtt gaaacagtag ggaactgcca
     9841 agtggtgcgt gatgcgaata atgctcctat ctattcacaa attaccaaag gctgctacac
     9901 agaaagtcac tatacggtag gtaaaagtga tacgctttat ttaatcggtt atctaaccgg
     9961 caccaatgcc aatcaaattg cgcagctaaa tggtttaaat accggctctc cactgaaagt
    10021 cgggcaagtc ttacgtgttc gttaataggc agagaaaatg aacagaaccc ttttagcatt
    10081 tgcaatttct tgtgcatttt tgaccgcttg tagctcgcct actcagccac aagttgaaga
    10141 tccaaatatg ttacctgcag gcacattaca accgattgaa ggctcaggcg caagccaaga
    10201 cagttacagt tggccgtctg atattcaatc aacgtcaatg cctgacagta tgaaataatt
    10261 ttaggacaat catttaatga aaaaacaatt tattcttctc cctttagtct ctgctgtttt
    10321 ggcagcctgt tcttcaaaca atccagcacc ggtggtgaat gcttccggta ataacgaatt
    10381 aagcccgggc gtgatgcagc ctgttgatgt gagtgcaggt gcaattaatt caagcggtgg
    10441 ctggcagtct gatattcaat ctgcaccaat gccaagctcg atgaatacca ctccggtgca
    10501 aacaatgccc cagccggttt cagtgccaca acctgtggcg actgtaccgc agccaattac
    10561 caatccggtg attaccgaac aaccaccaca gccgacaacc acgaccaaaa tcgtgaaaaa
    10621 aacgaaaacg gtcgagaaaa aagtggatca aaattttgag atcccgcgtg atgccaacaa
    10681 tgccccaatg tacaaccaaa ttcaaaaagg gttctacgat ggctctacct ataccgtacg
    10741 caaaggcgac acaatgtttt tgattgccta tattgtgggt aaagatgtaa aagaaatcgc
    10801 cgctcttaat aatatgagcg aaccttacca attaagcgta gggcaaaaac tcaaaaccgg
    10861 caaatctgca acggaaacta ttaccgtgga agaaaaagtc accgtaccgg ttgaaccaca
    10921 aattacctac caacaaggtg caaacggcac aacctatgct tctggcggta atattactgg
    10981 gccggtaaaa gcaggcacag ccaacgctga tgtcacgccg gttaataacg gtattaaagc
    11041 aacaactgca actgctccgg cagtaactgc ttctgtcggt acagtaaatg caacagagga
    11101 ccgtaccgcc tccaccattc gtgctataga tggcacagca agtggtgccg tacaaagtag
    11161 taatactgtt tctgcaccag cttcatcaat taaatggcaa tggccaacca gcgggcgtgt
    11221 aatttcaggc ttctcctcag cagaaggagg taataaaggc attgatattg ccggcaataa
    11281 aggacaagat gtacgtgctg ccgccgcagg taaagtggta tatgccggta atgccttgca
    11341 gggttacggt aacttaatca tcatcaaaca taccgatgat ttcttaagtg cttatgcaca
    11401 caacaatact attgaagttg atgagcaaga aacggtgaaa gccggtcaga aaatcgcgac
    11461 tttaggtagt acaggaacca atacgaataa attacatttt gaaattcgtt acaaaggaaa
    11521 atctgtcgat ccggctcgtt atttaccaag aaaataagag taacttttta gcataatccg
    11581 accgcttgtg tactacaagc ggtcaatttt taggaaaatt atgcaaatga aaaaatatat
    11641 cgcacacatg ataagttcgt tactgctgat agcttgttcg gactaccgag caacaccaac
    11701 tagcccaagc gatgcttaca cttgctctcc ttcttttcgc tactctttct taaaaaaaac
    11761 gtgcatacct ttattcagta tagccgacat tcaattattg gatccaaccg agccttcaca
    11821 tcgcatttat attttgttat ctgaagatcg atttcaagcc gaagtcttct caacgaagct
    11881 ctctgaatct tctatgttat tggaggcggt taaaggaggc tatatttcaa aagacggaaa
    11941 agttcgttta ctcaaaaaag ctgaaggttg gaaaatttac tatgagtgag actaaaatac
    12001 gttgtgattg gtgtaaaagc agtgaaattt atatgaaata tcacgacgaa gaatggggaa
    12061 aaccggagtt tgacagcctc aaactgtttg agaaaatctg ccttgaaggg caacaagccg
    12121 gactttcttg gattacggtg ttgaaaaaac gggaagccta ccgccaagca ttccatcagt
    12181 ttaatcctga aaaaattgcc aaaatgggtg aagaagaaat tgatttgtta atgcaaaaca
    12241 ctagcttaat ccaccaccga gcaaagttag aagccattat aaaaaatgcg aaagcttata
    12301 tcacaatgca gcaaaatggc gaagacttca gtcggtttat ttggtctttt gtgaattatc
    12361 agccgcaaat taatgatgta ccaacacttg aagctgttcc tgctcataca gaaacctcaa
    12421 aagcaatgtc aaaagcgttg aaaaaacgtg ggtttgtgtt tgtaggcgag actacttgct
    12481 atgcttttat gcagtcaatg gggctggtta atgaccattt gaataattgc gtgttcaaac
    12541 ataaataaac acaaaaaatt aggcgaccat ttggtcgcct aacattaatt cagattattg
    12601 attgttttga acgtaatcaa ttgctgattg aactgtagta attttttcag cttcttcatc
    12661 tggaatttcg atatcgaatt cttcttctaa agccattact aattcaacag tgtctaaaga
    12721 atctgcacct aaatcttcaa tgaatgaagc ttctggtttt acatcttctg ctttcgcgcc
    12781 aagttgatca acaatgattt tttttacgcg ttcttcaatg ctcattttgt ttttcctata
    12841 gtgaatatcg cataagtgcg agagttaaat agtgtaatcg aaataagttt aaatacaacc
    12901 ttttataaaa aggtcaaacc agactattcc tatacaccag aatatatctg tttttccatt
    12961 ctatcattat gtttgtgttt aatctataga gataaatcaa atttcatttg attaccctac
    13021 tttccgtaat tcatctcgca tttgtgcaat cactgcttga taatctggct ggtcaaaaat
    13081 cgcagaacct gccacaaaca tatctgctcc tgcggctgca atgtcggcaa tgttatttat
    13141 tttcacacca ccatcaacct ctaagcgaat gttataaccg ctctcatcaa tacgacgacg
    13201 gacttctttc aatttatcta aggtggacgg aataaatgat tgtccgccaa agcccggatt
    13261 cacagacatc aataaaacca cgtcaacttt atccatcaca taatctaaat agctcaatgg
    13321 cgttgccggg ttaaacacca agcctgattt acaacctaaa tcacggataa gctgtaaatt
    13381 tcgatctata tgttccgttg cttcagggtg gaatgtaata taatccgctc cggctttggc
    13441 aaaatccgga atcaaacgct caaccggttt taccattaaa tgcacatcaa tcgggcattg
    13501 aatgccgtaa tcacgtaagg ctttgcagat cgccggcccg aaggtcaaat tcggcacgta
    13561 gtggttgtcc attacatcaa agtgaattaa gtctgcaccg gcttttaaca cctctgccac
    13621 atcatcaccc aagcgagcta aatcggcaga taaaatagac ggtgcaatta aataaggatt
    13681 cttactcata tttcctcact atcaaaaaat aaatctacgt tagattacca taattttaac
    13741 agcgaaaaga gtctctcttc ctaaaaatcc gtttttttac aaaacaccat aaaaaagtta
    13801 tggaattgt
//
LOCUS       DANLZC010000060        13711 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.060, whole genome shotgun sequence.
ACCESSION   DANLZC010000060 DANLZC010000000
VERSION     DANLZC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13711
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.060"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(14..730)
                     /gene="rph"
                     /locus_tag="PXE44_001371"
     CDS             complement(14..730)
                     /gene="rph"
                     /locus_tag="PXE44_001371"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL5588883.1"
                     /translation="MRPNNRANNQVREIKITRNYTRYAEGSVLIEFGETKVLCNATVE
                     ESVPRFLKGQQQGWVTAEYGMLPRATHSRTQREAAKGKQGGRTMEIQRLIARSLRAVV
                     DLKALGERTVTVDCDVIQADGGTRTASITGACVALYDAMNKLVANGTLKTNPMKGLVA
                     AISVGIVAGEAVCDLEYLEDSNAETDMNVVMVEDGRLVEVQGTAEGEPFSHQELLTLL
                     DLAKQGISQLVEAQRKALAE"
     gene            1011..1874
                     /locus_tag="PXE44_001372"
     CDS             1011..1874
                     /locus_tag="PXE44_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL5588884.1"
                     /translation="MIYSMTAFAHLEMKKEWGNAVWEIRSVNQRFLETYFRLPEQFRN
                     LEMTLRERLRSALTRGKVECSLRIDLSKTQNSEIALNKEYAAQVVQSLKWLKETANEG
                     EINLVDVLRFPGVVDNESQDLDQIAQDLLAGFEQILADFIAMRAREGENVQALILQRL
                     EAISVEAAKVQAQMPEILQWQKDRLQQRFDELNLQLDPQRLEQEMVLLAQRVDVAEEL
                     DRLQLHVKETTNILKKGGAVGRKLDFMMQELNRESNTLASKSINADVTNSAVELKVLI
                     EQMREQIQNLE"
     gene            1883..2773
                     /locus_tag="PXE44_001373"
     CDS             1883..2773
                     /locus_tag="PXE44_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HDL5588885.1"
                     /translation="MAMNFKQVSQSYPNLCVPLSQYCLIEIAGVDAEKYLQGQLTCDV
                     AKISVGEHTLTSHCDPKGKMSALLRLYRAENEKFMAIIHRSLLPEALTQLKKYAVFSK
                     VTFTELDTPLYGMAGEAAFAKLSENMTALRLTTGQPRAIVWGEELETTADEQLWTLMD
                     IQDGIPVLLQQNQFELIPQATNLQALESAISFTKGCYIGQETVARAKYRGANKRALFT
                     LAGNFESEISLPEVASAIEMQLGENWRATGTVLTSATDENTLWVQVVLNKDVEPNTKF
                     RANGVALEMVELPYSLAENE"
     gene            complement(2805..3065)
                     /locus_tag="PXE44_001374"
     CDS             complement(2805..3065)
                     /locus_tag="PXE44_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HDL5588886.1"
                     /translation="MALLITHKCTNCDMCLPECPNEAISVGDDIYIIDPVLCTECVGH
                     YEKPTCQKVCPITNCIIKDPDNIETEEQLWERFVMIHHPDQI"
     gene            3295..3714
                     /gene="rbsD"
                     /locus_tag="PXE44_001375"
     CDS             3295..3714
                     /gene="rbsD"
                     /locus_tag="PXE44_001375"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL5588887.1"
                     /translation="MKKTLNLNAQLSHSIATLGHTDSLTVCDAGLPIPNTIERIDLAL
                     TAGVPSFLQTFDVVVSELFVERVLLAEEIKHKNPQVLAALLERLTQLEQTQQNKIQID
                     YVNHEIFKEQTHYSKAVVRSGECSPYANIILYSGVPF"
     gene            3724..5220
                     /gene="rbsA"
                     /locus_tag="PXE44_001376"
     CDS             3724..5220
                     /gene="rbsA"
                     /locus_tag="PXE44_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDL5588888.1"
                     /translation="METLLKMSGIDKEFPGVKALSNACLSVYGGRVMALMGENGAGKS
                     TLMKVLTGIYSKDAGSISYLGKEVTFKGPKDSQEAGISIIHQELNLVGNLTIAENIFL
                     GRESKTAWGAIDWKKMYAEADKLLARLGVKHSSHQLCAELSIGEQQMVEIAKALSFES
                     QVIIMDEPTDALTDTETASLFKVIRELKAENRGIVYISHRLKEIFEICDDVTVLRDGQ
                     FIGERVIADIDEDQLIEMMVGRRLEEQYPHLHQERGELLLEVKNLSGSGVNNVSFQLH
                     KGEIVGISGLMGAGRTELMKVLYGALPKTAGSIELNGKAISNHCPQDGLNNGIVYISE
                     DRKGDGLILGMSVKENMSLTSLDHFSKAGSIRHDAEKLAVDDFIEMFNIKTPSREQQI
                     GLLSGGNQQKVAIAKGLMTRPNVLILDEPTRGVDVGAKKEIYQLINEFKKDGLSILMV
                     SSDMPEVLGMSDRILVMHEGQISAEFSRAEATQEKLLAAAIGKSTGLN"
     gene            5230..6198
                     /gene="rbsC"
                     /locus_tag="PXE44_001377"
     CDS             5230..6198
                     /gene="rbsC"
                     /locus_tag="PXE44_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDL5588889.1"
                     /translation="MATQTKQFQLGRFLIEQRSFVALFILIVSVSMINPDFFSVDNIL
                     NILRQTSVNAIIAVGMTFVILIAGIDLSVGSVLALTGAVAATLVGSELPILLVIPLVL
                     LLGTAFGGISGAIVAKGKVQAFIATLVTMTLLRGITMVYTDGRPISTGFSDQADAFAF
                     IGTGYLFGIPVPIWIMAVVFGLAWYILKHTPIGRYIYALGGNESATQLSGINVNKIKI
                     FVFAVSGFLSALAGLIVTSRLSSAQPTAGVSYELDAIAAVVVGGTSLMGGKGRVMGTL
                     VGALIIGFLNNALNLLDISSYYQMIAKALVILAAVLADNYLGTKKV"
     gene            6221..7099
                     /gene="rbsB"
                     /locus_tag="PXE44_001378"
     CDS             6221..7099
                     /gene="rbsB"
                     /locus_tag="PXE44_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDL5588890.1"
                     /translation="MKKLTTIASAVMLSLSVAATASAKETIALAVSTLDNPFFVSLKD
                     GAQKKADELGYKLVVLDSQNDPAKELSNVEDLTVRGAKVLLINPTDSEAVGNAVAIAN
                     RNKIPVITLDRGAAKGEVVSHIASDNVAGGKMAGDFIAQKLGSGAKVIQLEGIAGTSA
                     ARERGEGFKQAIEAHKFNVLASQPADFDRTKGLNVTENLLSSKGDVQAIFAQNDEMAL
                     GALRAVSSAKKEVVIVGFDGTDDGVKAVESGKLAATIAQQPELIGSLGVETADKVLKG
                     EKVEAKIPVDLKVISK"
     gene            7220..8149
                     /gene="rbsK"
                     /locus_tag="PXE44_001379"
     CDS             7220..8149
                     /gene="rbsK"
                     /locus_tag="PXE44_001379"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL5588891.1"
                     /translation="MKKLTVLGSINADHVISVPYFAKPGETLTGHSCHIAYGGKGANQ
                     AVAAARLGAKVSFIGCIGSDDIGKAMKSAFEQDGIDTRSIKTIPNEMTGIAMIQVAES
                     GENSIVISAGANGHLDETVVAKCAAEITQADYLLMQLETPLPAIVQAAKLAQQNGTKV
                     ILNPAPARALPDELLNLLDMITPNETEAEILTGVKVVDEASAIQAAQVFHQKGIEKVL
                     ITLGSKGVFVSENGVGKIVAGFRVQAVDTTAAGDTFNGALVTAMLEDKPLDQAIRFAH
                     AAAAISVTRKGAQPSIPTRQETLDFLSQQAVSF"
     gene            8163..9173
                     /locus_tag="PXE44_001380"
     CDS             8163..9173
                     /locus_tag="PXE44_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5588892.1"
                     /translation="MATMKDIARLAQVSTSTVSHVINNSRFVSEEIRERVQRVVKELN
                     YTPSALARSLKVNETKTIGMLVTATGNPFFAEVMAGVEQYCQQHQYNLIIATTNGDTE
                     RLQHHLQMLMQRKVDGLLLMCGDTRLNADESLNIPLPLVVLDWWFTELNADKIFENSI
                     SGGYLATKTLIEAGHSKIAIITGNLKKSLAKNRLEGYKKALAEANIPLNPNWVFESHF
                     DFAGGVNGMNALLAQSEKPTAVFACSDTIALGAYQAIWQQGLRIPQDISIIGYDDIEL
                     AKYLSPPLSTISQPKAELGRLAVETLLKRIRFKSTDFQTIMLEPELVLRHSIQTINKN
                     AE"
     gene            complement(9209..9838)
                     /locus_tag="PXE44_001381"
     CDS             complement(9209..9838)
                     /locus_tag="PXE44_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5588893.1"
                     /translation="MTAPTQILLIDDHPLMRQGMRQLLELDHQFSVVGEASNGTDGIK
                     LALRLEPDVIILDLNMKGLSGLDTLRSLRAQGIDARIIVLTVSDEQSDVFALMDAGVD
                     GYLLKDTDTPELLDNIRKAAAGEVVLSETVRQHLLNRQPENGPLSCLTDRELDVLQWL
                     ATGMSNKQIAAQLFISEETVKVHIRNLLRKLNVHSRVAATVLYLEQQKG"
     gene            10036..12195
                     /gene="rlmKL"
                     /locus_tag="PXE44_001382"
     CDS             10036..12195
                     /gene="rlmKL"
                     /locus_tag="PXE44_001382"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDL5588894.1"
                     /translation="MTNQTTYFATAARGFEEILKTELEQICAAECKVAQGGVHFTTTQ
                     KGAYQALLHSRLASRILLPLISTKIFSDSDLYASIVSFNWADLFDPRDTFYIDFSGTN
                     REIRNTQFGAMRVKDGIVDYFERKGFARPTVDKDNPDVRIHVYLDRENVVVSLDLSGD
                     ALHIRGYREETGKAPLRETLAAAIILRSGWKVGTPLVDPMCGSGTLLIEAAQMAANIA
                     PQLHRKHWGFNAWKGHQQAMWKAVLDEAFRNVELGAVGENCNSSLQKMFFGFDLDHRV
                     LTKAKQNAKNAGVDHLIQWQQGDVAALKNPIPEKIGTVVCNPPYGERLGTTPALIALY
                     SVFGQRLKQQFAGWNASIFSGEPELLNCLRLRSARQFKAKNGPLDCVQKNYSISEHNK
                     RSDLDENLQFEQNAQVAPDFANRLTKNIKKIEKWAKQQGIDAYRLYDADLPEYNLAVD
                     RYGDHIVVQEYQAPKNIDEQKARQRLLDAVSATLYVTGVETNKLVLKVRQKQKGTNQY
                     EKLANKGEYFYVNEYGAKLWVNLTDYLDTGLFLDHRLTRKMVGEMAKGKTFLNLFAYT
                     GSATVHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLSLRHHRLFQADCLQWLAECRE
                     RFELIFVDPPTFSNSKRMENSWDVQRDHIELMKQLKRILTADGTIVFSNNKRGFKMDF
                     DGLAALGLTAENISHKTLPLDFERNPQIHNCWIVKNV"
     gene            12372..12851
                     /gene="trmL"
                     /locus_tag="PXE44_001383"
     CDS             12372..12851
                     /gene="trmL"
                     /locus_tag="PXE44_001383"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL5588895.1"
                     /translation="MLDIVLYEPEIPQNSGNIIRLCANCGFRLHMIEPFGFGWDDKKL
                     RRSGLDYHEFVDIQKYKNFEDFLERAKPKRLFALTTRGEQNHSDVKYELGDFLMFGPE
                     SRGIPKPLLAEMPMSQKIRIPMCKDSRSMNLSNSVAVVVYEAWRQFGYQNAVAAQSI"
     gene            complement(12945..>13711)
                     /locus_tag="PXE44_001384"
     CDS             complement(12945..>13711)
                     /locus_tag="PXE44_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL5588896.1"
                     /translation="EKDKWYFSIIDVIAILTESLDPSAYWRKLKQRLKAEGNETVTNC
                     HGLKMPSADGKMRLTDVADTEQLFRLIQSIPSPKAEPFKQWLAKVGYERLQEINDPSQ
                     SLDRARENWQKMGRSEKWIQQRMMGQETRNKLTDYWKSSGVEQPQDFAFLTNVIHQEW
                     SGLSVQKHKSLKGLKSQNLRDHMSEAELLFTALAELSSRQIAESMQAVGVDENAQAGK
                     QGGNIAKNARQELENKTGKSVVTGENFLPPAKNEEE"
BASE COUNT         3948 a         2698 c         3192 g         3873 t
ORIGIN      
        1 aaagagaagt gaattattca gctaacgctt tacgttgagc ttcaactagc tggctaatgc
       61 cttgcttcgc taaatctagc aatgtcagta attcttggtg cgagaacggt tcgccttctg
      121 ccgtgccttg tacttccact aaacgaccat cttcgaccat caccacgttc atatcagttt
      181 cagcgttgga atcttccaga tattctaaat cgcacaccgc ttcgcctgct acaataccga
      241 cagaaattgc tgcgactaaa cctttcattg ggttagtttt tagtgttcca ttagctacta
      301 acttattcat tgcatcgtac aatgctacgc acgcaccggt aattgaggcg gtgcgagtgc
      361 ctccatcggc ttgaataaca tcacaatcta cggtaacggt acgttcgcct aaggctttta
      421 aatctaccac cgcacgcaga gaacgggcaa tcaagcgttg gatttccatt gtgcgtccgc
      481 cttgtttgcc ttttgccgct tcacgctgag tgcgagagtg ggtggctcgt ggcaacatac
      541 catattctgc ggtaacccaa ccttgttgct gaccttttaa aaagcgtggc acgctttctt
      601 ctaccgtagc gttacacaat actttggttt cgccaaactc aattaatacc gagccttcgg
      661 catagcgagt gtagttacgg gtaattttaa tttctctaac ttggttgttt gctcgattat
      721 ttggacgcat tttcttttcc ttttgctaaa atttttacca ttttagcaaa gaacacagta
      781 aaatgtgatt tttctttagc aaagaattta catagccctc gccccttgtg ggagagggac
      841 ggatttttct tcgttcagaa gaaaaatcag ggagaggggt aagcggttat ttttatctca
      901 aattttacaa attcctccct ctctctgaaa aatatttctg aacgaaatat ttttcggtct
      961 ctctccctca aggggagaga gctttactta taaaaacaca aggaatacca atgatttata
     1021 gtatgacagc atttgcccat ttagaaatga aaaaagagtg gggcaatgcg gtgtgggaaa
     1081 ttcgctcggt aaatcaacgc tttttagaaa cctattttcg tttgccggag cagtttcgca
     1141 acctagaaat gaccttgcgt gagcgtttac gttctgcact gacacgaggc aaagtagagt
     1201 gtagcttacg cattgattta agcaaaaccc aaaacagtga aattgcgtta aacaaagaat
     1261 atgccgcaca ggtggttcaa tcattaaaat ggttaaaaga aaccgcaaat gaaggcgaaa
     1321 tcaaccttgt tgatgtgcta cgtttcccgg gcgtggtgga taatgaaagc caagatttag
     1381 accaaattgc tcaagattta ttagccggct ttgaacaaat tctggcagat tttatcgcaa
     1441 tgcgagccag agagggcgag aacgtgcaag ccctgatctt acaacgttta gaagcaattt
     1501 cagttgaggc tgcaaaggta caagcccaaa tgccggaaat tctgcaatgg cagaaagatc
     1561 gtttacaaca acggttcgat gaactgaatt tacagcttga tccacaacgc ttagaacaag
     1621 aaatggtgtt acttgcccaa cgtgttgatg tggcggagga attagaccgc ttgcaattac
     1681 acgttaaaga aaccaccaat atcctgaaaa aaggcggtgc ggttgggcgt aaactggatt
     1741 ttatgatgca agaactaaac cgtgaatcca acacgctcgc ctcaaaatcg attaatgcgg
     1801 acgtgaccaa ttcggcggtg gagctgaaag tgttgatcga acagatgcgt gaacaaattc
     1861 agaatttaga ataggacaga ttatggcaat gaatttcaag caagtgagcc agagctatcc
     1921 aaatctctgc gtgccgcttt ctcagtattg tttaattgaa atcgcagggg tagatgctga
     1981 gaaatatctt caagggcaat taacctgcga tgtggcaaaa atttcggtgg gcgaacatac
     2041 gctaaccagc cattgtgatc cgaaaggcaa aatgagtgcg ttactgcgtt tgtatcgtgc
     2101 agaaaatgag aaatttatgg cgatcattca ccgcagctta ttgcctgaag ccctgacgca
     2161 acttaaaaaa tatgcggttt tttcaaaagt gaccttcacg gaattagaca ccccacttta
     2221 tggtatggca ggtgaagcag catttgcaaa attaagcgaa aatatgaccg ctttgcgttt
     2281 aaccacagga cagcctcggg ccattgtttg gggcgaagag cttgaaacga ctgccgatga
     2341 acagctctgg acattaatgg atattcaaga cggcattcct gttctcttgc aacaaaacca
     2401 gtttgagctg attccacagg caaccaattt acaggcactt gaatctgcga tttcattcac
     2461 caaaggttgc tacatcgggc aagaaacggt agcgagagcg aaatatcgtg gggcgaataa
     2521 acgtgcttta tttacccttg ccggcaactt tgaaagcgaa atcagcttac cggaagttgc
     2581 ttctgcgatt gaaatgcaac tgggcgaaaa ttggcgagca accggcactg tgttgaccag
     2641 tgcgactgat gaaaataccc tgtgggtgca agtggtgctg aataaagatg ttgaacctaa
     2701 caccaagttt agagccaatg gtgtagcgtt ggaaatggtc gagttacctt atagtttggc
     2761 agaaaatgag taaaacgaac ggtcatcagg gtatgaccgt ttttttatat ctgatcaggg
     2821 tgatgaatca tcacaaaacg ttcccacaac tgctcttcgg tttcgatatt atcaggatct
     2881 ttgataatgc agttggtaat cgggcaaact ttctggcaag tcggcttttc ataatgcccg
     2941 acacattcgg tacataacac cggatcgatg atataaatat catcgcccac cgaaatcgcc
     3001 tcgttcgggc attccggcaa gcacatatca cagttggtac atttgtgggt aattaataaa
     3061 gccatagttt atattgagaa aataaaaatt aaacaagcgg tcgattttgc aaaaaaattt
     3121 gcaaatctta ccgcttgtca ctaaatgcta cctattataa agcaaaataa tttgaactac
     3181 ttcacatttt tgcaatttgg ttgtttatcc gattaagaaa attttttatg atgccatcga
     3241 aacgtttcga tggagtattt tccaacgtga tggagcgaaa ataaaggggt tagtatgaaa
     3301 aaaacgctaa atctaaatgc acaactttcg cacagtattg cgacactcgg gcataccgat
     3361 agtttaacgg tttgcgatgc cggtttgccg attccaaaca caatagagcg gatcgatttg
     3421 gctttaaccg ccggtgtgcc aagtttttta caaacatttg atgtagtagt tagtgaatta
     3481 tttgttgaac gagtgctact tgctgaagaa attaaacata aaaatccgca agtgttagca
     3541 gctcttttgg agcgtttaac ccaattagaa caaactcaac agaataaaat tcaaattgat
     3601 tatgtgaacc acgaaatctt taaagagcag acacattaca gtaaagctgt tgtgcgttcg
     3661 ggtgagtgtt caccgtatgc caatattatt ttatattcgg gcgtgccgtt ttagggtgga
     3721 atgatggaaa ccttattaaa aatgagcggg atagataaag aatttcccgg cgtgaaagcc
     3781 ttgagcaatg cgtgcttatc tgtttacggt gggcgagtaa tggcattaat gggcgaaaat
     3841 ggggcaggta aatccacctt gatgaaagta ttgacgggga tttatagcaa agatgccggc
     3901 tctattagct atttaggcaa agaagttacc tttaaaggcc cgaaagattc acaagaagcc
     3961 ggtattagca ttattcacca agagctgaat ttagtcggta atttaaccat tgctgaaaat
     4021 atttttttgg gccgtgagtc caaaaccgct tggggagcta ttgattggaa aaaaatgtat
     4081 gccgaggcgg ataaactgct tgcccgactt ggggtaaaac acagtagcca tcagctttgt
     4141 gcggagttat ccattggcga gcagcaaatg gtggaaattg cgaaagccct gagttttgag
     4201 tctcaagtaa ttattatgga cgaaccgacc gatgctttaa ccgatacaga aacagcatcg
     4261 ttgtttaaag ttattcgaga gttaaaagcg gaaaatcgag gcattgttta tatttcccat
     4321 cgtttaaaag agatttttga aatttgtgat gatgtgacgg tgctaagaga cgggcaattt
     4381 atcggcgagc gagtaattgc cgatattgat gaagatcagt taattgaaat gatggttggt
     4441 cgccgtttgg aagagcaata tccgcattta caccaagagc gtggcgagtt attacttgaa
     4501 gtgaagaatt taagtggaag tggggtgaat aatgtgtcat tccaattaca caaaggtgaa
     4561 attgtgggta tttccggctt aatgggggca gggcgtacag aattaatgaa ggtgctttat
     4621 ggtgcattgc cgaaaacagc cggctcgata gagttaaatg gaaaagcgat ttccaaccat
     4681 tgtccgcaag atgggttgaa taacggcatt gtgtatattt cagaagatcg taaaggcgat
     4741 ggtctgattt tgggaatgtc ggtaaaagaa aatatgtctt tgacctctct cgatcatttc
     4801 tccaaagccg gcagtattcg ccacgatgca gaaaaattgg cggtcgatga ttttattgag
     4861 atgtttaaca ttaaaacccc aagtcgggag caacaaatcg ggctgctttc cggtggtaat
     4921 cagcaaaaag tggcgatcgc taaagggttg atgacccgcc cgaatgtgct gattttagac
     4981 gagccgacac ggggtgttga tgtgggggcg aaaaaagaga tctatcaatt aattaatgaa
     5041 tttaaaaaag atgggcttag tattttgatg gtttcgtccg atatgccgga agtgttggga
     5101 atgagcgacc gcattttagt gatgcacgaa gggcaaatca gtgcagaatt ttcacgagca
     5161 gaagcgacac aagaaaagct actggcagca gctattggca aaagtacagg attaaattaa
     5221 ggtgtgttta tggcaactca aacaaagcag tttcaacttg gcagattttt aatcgagcaa
     5281 cgttcttttg tagcgttatt tattttaatt gtcagtgttt caatgattaa ccctgatttt
     5341 ttcagcgtgg ataatatttt aaatattcta cgccaaacct cggtgaatgc gattattgcg
     5401 gtcggaatga cctttgtgat tttaattgca ggcattgatt tatcggtcgg ttcggtttta
     5461 gccttaaccg gtgcggttgc agcgacctta gtcggttctg aattgccgat tttgttggtg
     5521 attccgctgg ttttattatt agggacggca tttggtggta ttagtggggc gattgttgct
     5581 aaaggtaaag tgcaggcatt tattgcgact ttggtcacaa tgaccttatt gcgtgggatt
     5641 acgatggttt ataccgatgg cagaccgatt agcaccggct tttctgacca agcagacgcc
     5701 tttgcgttta ttgggacagg ctatttattt ggtattcctg tgccgatttg gattatggcg
     5761 gtagtgtttg ggttagcttg gtatattttg aaacacaccc cgattgggcg ttacatttac
     5821 gctttaggtg gcaacgaatc ggcaacccag ctttcaggca tcaatgtgaa taaaattaag
     5881 atcttcgtat ttgccgtgag tggtttttta tctgctcttg ccggcttaat tgtgacttca
     5941 cgtctctctt ctgctcagcc aacggcaggt gtgtcctatg agttagacgc aattgcagcg
     6001 gtagttgttg gtggaaccag tctaatgggc ggtaaagggc gtgtaatggg aacgcttgtc
     6061 ggggcattaa ttatcggttt cttaaacaat gcccttaatt tattggatat ttcttcatat
     6121 tatcagatga ttgctaaagc gttggttatt ttagcagctg tattagctga taattatcta
     6181 ggcactaaaa aagtttaatc tatctttaaa ggagatttct atgaaaaaat taactacgat
     6241 tgcatcagcg gttatgttaa gtttatcggt tgcagccact gcttcggcaa aagagactat
     6301 cgctcttgca gtatcgactt tagataaccc attctttgtg agccttaaag acggtgcaca
     6361 gaaaaaagca gatgaattag gctacaaatt agtggtgctt gattcccaaa atgacccggc
     6421 taaagagctt tccaatgtgg aagacttaac ggtgcgtggg gcaaaagtgt tattgattaa
     6481 cccaaccgat tcggaagcgg tcggaaatgc ggtggctatt gcaaatcgca acaaaattcc
     6541 ggttattaca cttgaccgtg gtgcagcaaa aggcgaagtg gttagccata tcgcttccga
     6601 taacgttgcc ggcggcaaaa tggcgggaga tttcattgct cagaaattag gctcaggggc
     6661 gaaagtgatt caattagaag gcattgccgg cacttccgcc gctcgtgaac gtggtgaagg
     6721 ctttaaacaa gcgattgaag cacataaatt caatgtatta gccagtcaac cggctgattt
     6781 tgaccgcact aaaggcttaa atgtgactga aaacttgctc tcaagcaaag gggacgtaca
     6841 agctattttt gctcaaaatg atgaaatggc actaggagct ttgcgtgcag tgagttcagc
     6901 gaagaaagag gttgtaattg tgggctttga cggcacagat gatggcgtaa aagccgttga
     6961 aagcggtaaa cttgccgcaa cgattgctca acaacctgaa ctgattggct cattaggcgt
     7021 agaaactgcc gataaagtgc taaaaggcga aaaagtggaa gcaaaaattc cggttgattt
     7081 aaaggttatt tcaaaataat tatacttgaa gatagcgttc gtctctgacg aacgctttga
     7141 attaataaaa tagcactcgt cagagactag tgctatcaga atcctagttt tgttgtacta
     7201 ggtaatttga gggcttaata tgaaaaaact caccgttctc ggtagcatta acgctgatca
     7261 cgtgatttcc gttccctatt ttgccaaacc gggggaaacg ctcaccggac atagctgcca
     7321 cattgcttat ggcggtaaag gggcaaatca ggcggtggcg gcagcacgtt tgggagctaa
     7381 ggtttctttc atcggttgta ttggcagtga tgacatcggc aaagcaatga aatcagcctt
     7441 tgagcaagat ggcattgaca ctcgctcgat taaaacgatt ccgaatgaaa tgaccggcat
     7501 tgcaatgatt caagtggcgg aatcgggcga gaacagtatt gtgatttcag ccggagcgaa
     7561 cggtcatttg gatgaaacgg ttgtggcaaa gtgtgcagcc gaaatcacac aggcggatta
     7621 tttgctaatg cagctagaaa caccgttgcc ggcgattgtg caagcagcaa aattggctca
     7681 gcaaaacggc acgaaagtaa tattaaaccc tgcgcctgct cgagcgttac ccgatgaact
     7741 gttaaactta ctggatatga ttaccccaaa tgaaacggaa gcggaaatat taaccggtgt
     7801 gaaagttgtt gatgaagcca gtgcgattca agccgcacaa gtgtttcacc aaaagggcat
     7861 cgaaaaagtg ttgattacac ttggctcaaa aggggtattt gtaagcgaaa acggtgtagg
     7921 caaaatcgtt gcaggcttcc gtgttcaagc ggtggatacc actgccgcag gggatacttt
     7981 taacggagca ttagtgactg caatgttaga agataaaccg ctggatcaag ccattcgttt
     8041 tgctcacgca gcggcagcga ttagtgtgac tcgcaaaggg gctcaacctt ctattccgac
     8101 ccgtcaggaa acactggatt ttctaagcca acaagcggtg agtttttagg aaaattttgc
     8161 aaatggcaac aatgaaggat attgcacggc ttgcacaggt ttccacctcg accgtttctc
     8221 acgttatcaa caatagccga tttgtcagcg aggaaattcg ggaaagagtg cagagggtgg
     8281 tgaaagaact gaattacacg ccttctgcat tggctcgtag cctcaaagtg aatgaaacta
     8341 aaaccatcgg aatgctggta acggcaaccg gcaatccgtt ttttgcggag gtgatggcgg
     8401 gggtggagca atattgccag caacatcaat ataacctgat tattgccact accaacggtg
     8461 acaccgagcg tttgcaacac catttgcaaa tgctgatgca acgaaaagta gatggcttgt
     8521 tactgatgtg tggcgatacc agattgaatg ccgatgagag cttgaatatt ccgctgccat
     8581 tggtggtgtt agactggtgg tttaccgagc tcaatgcaga taaaatcttt gaaaattcaa
     8641 tatccggcgg ctatctcgca acaaaaacct taattgaagc agggcattct aagattgcga
     8701 ttattactgg aaacctaaaa aaatcattgg caaaaaatcg tttagaaggc tacaaaaaag
     8761 cacttgccga ggcgaatatt ccactcaatc caaactgggt gttcgaaagc cattttgatt
     8821 ttgccggcgg ggttaatgga atgaatgctt tgcttgccca aagcgaaaag ccgacagcgg
     8881 tatttgcctg tagcgacacc attgctctag gggcttacca agcaatctgg caacaaggtt
     8941 tacgaatacc gcaagatatt tcgattatcg gctatgacga tattgaactg gcaaaatacc
     9001 tttccccacc gctgagtacc attagccagc ccaaagcgga actcgggcgg cttgcggtgg
     9061 aaactttgct aaaacgaatt cgttttaaat ccactgattt tcaaaccata atgcttgaac
     9121 ctgaattagt tttaaggcat tcaatccaaa caataaacaa aaacgctgaa taatgtgaag
     9181 ttattcagcg ttttttatcc gatttcaatt agcctttttg ctgttccaaa taaagcacgg
     9241 tggcggcaac tcgggaatgc acgtttaatt tacgcagtaa attgcggata tgcactttta
     9301 cggtttcttc ggaaataaag agctgagccg caatttgttt gttcgacata ccggttgcga
     9361 gccattgtaa aacatccagt tcacgatctg ttaagcaact taaaggacca ttttctggct
     9421 ggcggttgag taaatgttgg cgaacggttt cgcttaatac cacttcaccg gcagccgctt
     9481 tacggatatt atctagtagt tccggggtgt cggtatcttt taataaatag ccgtccacac
     9541 cagcatccat taatgcaaat acatcggatt gctcgtctga cactgttaaa acaatgattc
     9601 tggcatctat accttgagcg cgcaatgagc gtaaagtatc tagccctgaa agccctttca
     9661 tatttaaatc cagaataatc acatccggct caagtcgtaa tgcaagtttg ataccgtcgg
     9721 tgccgttgct tgcttcaccc actacactaa attggtgatc taattccaat agttgacgca
     9781 ttccctgacg cattaatggg tggtcgtcaa tgagtaaaat ttgagttgga gctgtcattt
     9841 atttctcctt tcctttctct taatcggtta attttgcaat tattaattca aaatagcaag
     9901 tttagggatt tactaaatgc cgcttttttg atcttagcta atttttaact aaaaaagaaa
     9961 ttgcaaaact ttgcaagaaa aagaccgctt gttcggctat aattactgcc aatttttatt
    10021 tcaagtgtga aaagaatgac aaatcaaaca acctattttg ccacggcagc ccgtggtttt
    10081 gaggaaatac taaaaaccga actggagcag atttgtgcgg cagaatgcaa agtcgctcaa
    10141 ggcggtgtgc attttaccac cacgcaaaaa ggggcttatc aggcattatt acacagccgt
    10201 ttagcctctc ggatcttact gccgctcatc agcaccaaaa tttttagtga tagcgattta
    10261 tatgcttcca tcgtgtcatt taactgggcg gatttattcg atccccgtga taccttttac
    10321 attgatttca gcggcactaa ccgagaaatc cgcaacaccc aatttggggc aatgcgagtg
    10381 aaagacggca ttgtggatta ctttgaacgc aaaggttttg cccgcccaac agtagataaa
    10441 gataatccgg atgttcgcat tcatgtttat ttggatcgtg aaaatgttgt ggtttcgctt
    10501 gatttaagtg gagatgcctt acatattcgg ggctatcggg aagaaaccgg taaagctccg
    10561 ttgcgtgaaa cattggcggc agccattatt ttgcgttccg gctggaaagt cggcacgcca
    10621 ttggttgatc cgatgtgtgg ttcaggcacg ttactgattg aagcagcaca aatggcagcg
    10681 aatattgccc ctcaacttca ccgtaaacat tggggcttta atgcgtggaa agggcatcaa
    10741 caagcgatgt ggaaagccgt gttagatgag gcattccgaa atgttgaatt gggtgcggta
    10801 ggggagaatt gcaattcgtc cctacaaaaa atgttcttcg gttttgatct cgatcatcgt
    10861 gtgctgacta aagcaaagca aaatgcgaaa aatgcagggg tagatcatct cattcaatgg
    10921 caacaaggtg atgtggctgc gttaaaaaat ccgattccgg aaaaaatcgg cacggtggtg
    10981 tgtaaccccc cttatggtga acgtttaggc acaacgccgg cgttaattgc tctttattcg
    11041 gtgttcgggc aacgcctaaa acagcaattt gccggttgga atgcctcaat ttttagcggc
    11101 gagcctgaat tgttaaattg cctgcgttta cgttcagccc gtcaattcaa agcgaaaaac
    11161 ggtccgttgg attgtgtgca gaaaaattac tccatcagcg agcataacaa gcggtcagat
    11221 ttagatgaaa atttacaatt tgaacagaat gctcaagtcg ctccggattt tgcaaatcgc
    11281 ttaaccaaaa atatcaaaaa gatcgaaaaa tgggcgaaac aacaaggcat tgatgcctac
    11341 cgtttatacg atgccgatct gcctgaatat aatttagcgg tggacagata tggcgaccac
    11401 attgttgtgc aggaatacca agccccgaaa aatattgatg agcaaaaagc ccgccaacgc
    11461 ttgttagatg cagtctctgc tacgctttat gtgacaggtg ttgaaactaa taaattagtg
    11521 ttgaaagttc gccaaaaaca aaaaggcacg aatcagtacg aaaaacttgc caataaaggc
    11581 gagtatttct atgtgaacga gtatggggcg aaactgtggg tgaatttaac cgattacttg
    11641 gatacgggtt tgttcctaga ccatcgctta acccgtaaaa tggtgggcga aatggcaaaa
    11701 ggcaaaacat tcctgaatct gtttgcctac acgggttcag ctacggttca cgctgctctt
    11761 aatggggcaa aatcgaccac tacagtggat atgtcgaaca cctatttgaa ctgggcggag
    11821 caaaatttag agctgaataa tttatcactt cgccatcatc gtttgttcca agccgattgc
    11881 ttgcaatggc tggcagaatg ccgtgagcga tttgagctga tttttgtcga tccgccgact
    11941 ttctccaact caaaacgaat ggaaaacagt tgggacgtgc aacgagacca tattgagtta
    12001 atgaaacagc ttaaacgtat tttaaccgct gatggtacca ttgtgttctc gaacaataag
    12061 cgtggcttta aaatggattt tgacggtttg gcagcattag gcttaacggc ggaaaatatt
    12121 tcgcataaaa ccttaccgtt ggattttgaa cgcaatccgc aaatccataa ttgttggatt
    12181 gtgaagaatg tatagataat accctcgacc cttgtgggag agggatagat ttttcttcgt
    12241 tcagaagaaa aatcagggag agggtaagaa gttactgatg aaagagttat ttccccctct
    12301 ctctgaaaaa tatttctgaa cgaaatattt ttcggtctct ctcccacaag gggagagagt
    12361 aaggctaaaa aatgttagat attgttttat acgaacctga aattccgcaa aacagcggaa
    12421 atattatccg cctttgtgcc aactgtggct ttcgtttgca tatgattgaa ccgttcggct
    12481 ttggttggga cgataaaaag ctccgccgtt cggggctgga ttatcacgaa tttgtggata
    12541 ttcaaaaata taaaaatttt gaagatttcc ttgagcgtgc taagcctaaa cgcttgttcg
    12601 ccttaaccac cagaggggag cagaaccaca gtgatgtgaa atatgaactt ggcgattttt
    12661 taatgttcgg gccggaaagc agaggcattc caaagccatt attagcggaa atgccgatgt
    12721 cgcaaaaaat ccgcattccg atgtgcaaag acagccgcag tatgaattta tctaactcgg
    12781 tggcggtggt tgtgtatgag gcgtggcgac aattcggcta ccaaaatgcg gtagcggcac
    12841 aatctattta acttgcaagc ggtgtgattt tggctatttt ttgcaagttt gtggtcaagg
    12901 ttgggggagg ctcgaccttg actaattaag aacatctatt gtgtctattc ttcttcattt
    12961 ttagctggag gtaaaaaatt ctctccggta acaactgatt tacctgtttt attctccaat
    13021 tcctgacggg catttttggc aatattgcca ccttgttttc ctgcttgggc attttcatca
    13081 accccaactg cctgcatact ttctgcaatt tgccgactgg aaagttcagc aagtgctgta
    13141 aataacaatt cagcttcgct catatgatct cgcagatttt ggctttttaa acctttaagc
    13201 gatttatgct tttgtacaga tagtccgctc cattcttgat gaatcacatt ggttaaaaat
    13261 gcaaaatctt gtggctgttc aacccctgac gatttccaat aatcggtcag tttatttcgg
    13321 gtttcttgtc ccatcattct ttgctgaatc catttttcac ttcgtcccat cttctgccaa
    13381 ttctcccttg ctcgatctag actttgactt ggatcgttta tttcctgtaa gcgttcataa
    13441 cctacttttg ctaaccattg tttgaagggc tctgctttcg gactagggat agattgaata
    13501 agtcggaaaa gttgttcggt atctgctaca tcagtcaaac gcatttttcc gtcagcagag
    13561 ggcattttca acccgtgaca atttgtcacg gtttcattgc cttctgcctt tagcctctgt
    13621 tttagcttac gccaataagc agagggatct aggctttcgg ttaaaatagc aatcacatca
    13681 ataatagaaa aataccattt atctttttct t
//
LOCUS       DANLZC010000061        13449 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.061, whole genome shotgun sequence.
ACCESSION   DANLZC010000061 DANLZC010000000
VERSION     DANLZC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13449
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.061"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..783
                     /locus_tag="PXE44_001385"
     CDS             <1..783
                     /locus_tag="PXE44_001385"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL5588897.1"
                     /translation="PLETDHDVSVWAARYIVDRINAFQPTKEKPFVLGLPTGGTPEKT
                     YKELIKLYQAGEVSFKNVVTFNMDEYVGLPKEHPESYHSFMFKHFFDHVDIPLENINI
                     LNGMAEDVNAECERYEEKIRFYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIKTLTE
                     DTRIANSRFFNNDVSQVPKFALTVGVGTLLDAEEVMILVTGHNKALALQACVEGAVNH
                     LWTISCLQLHRRGIVVCDEPATQELKVKTVKYFKELEKNVAR"
     gene            864..2006
                     /gene="nagA"
                     /locus_tag="PXE44_001386"
     CDS             864..2006
                     /gene="nagA"
                     /locus_tag="PXE44_001386"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL5588898.1"
                     /translation="MNHYALTNCVIYTGEQVLYQHALVVKEDKIDSIVLEENLPLDLQ
                     KIDLKGANLSAGFIDLQLNGCGGVMFNEEITTRTLEIMQETNLRSGTTSYLPTFITSP
                     DEGVKQAVAVMREYLSKHKNQALGLHLEGPYLSVAKKGVHRPEFIREISPEMLDFLCD
                     NGDVITKITIAAENPTADHIKRFNDAGIIVSIGHSNATFAQTKQRIAEGAAFATHLHN
                     AMSPVSNGREGGVVGAVLDSDIYTGIICDGLHVEWGNIRIAKKAKGDKLVIVSDAVAA
                     AGSDIESFIFVGKKVTVKDGKCYDEFGALGGAAITMIESIRNVVQHCDISLNEAIRMS
                     TLYPAKAIGVEETLGSLEAGKIANLAIFDNQFEVKATAVNGQLQWH"
     gene            2088..2402
                     /locus_tag="PXE44_001387"
     CDS             2088..2402
                     /locus_tag="PXE44_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HDL5588899.1"
                     /translation="MQFKDYLATFPTIDHLAGLNVINSGEVIQHIPAIEGKLGSLKLY
                     NALFAKFDGKLTASAAKQGLEWFAEHVEDAKNNVGKHPNIDLLFRVIEQDLSLSLEPI
                     VK"
     gene            complement(2481..3671)
                     /locus_tag="PXE44_001388"
     CDS             complement(2481..3671)
                     /locus_tag="PXE44_001388"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL5588900.1"
                     /translation="MTQAIESVLAELKRGVENIYSEEDLITKLKENRPLRIKLGADPT
                     APDIHLGHTVVLNKLRQFQQFGHEVYFLIGDFTGMVGDPSGKNSTRPPLSREDVLRNA
                     ETYKEQIFKILDPQKTKIVFNSEWLGKLGTEGMIRLASNYTVARMLEREDFKNRFTNQ
                     QPIAIHEFIYPLLQGHDSVELKADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPL
                     LVGLDGEKKMSKSLGNYIGVTEAPSEMFGKIMSISDDLMWDWYNLLSFRPLDEIAQLK
                     ADVENGKNPRDVKILLAKEIIARFHDEAAADAAEQEFINRFQKGAIPDEVPEFTFEGE
                     IGLAALLKEAGLVPSTSEAIRSAQQGGVKIDGEKVEDVKQTAQKGTFVYQVGKRKFAR
                     ITVK"
     gene            complement(4034..5338)
                     /locus_tag="PXE44_001389"
     CDS             complement(4034..5338)
                     /locus_tag="PXE44_001389"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HDL5588901.1"
                     /translation="MESIIALFKGMGLMHLELGQAIMIVISLVLLWLAIARKFEPLLL
                     LPIGFGGLMSNIPEAGMAMTALDNLLHSGSAEQLAIIAAKVNSTSLDLHSIKDAIALA
                     APSVQNELEVMAGDMGYTAGVLALFYKVAIGYGVAPLIIFMGVGAMTDFGPLLANPRM
                     LMLGAAAQFGIFATVLGALGLNWLGIIDFTLPQAAAIGIIGGADGPTAIYLASKLAPE
                     LLGAIAVAAYSYMALVPLIQPPIMKALTTEQERKIRMVQLRNVSNREKIIFPIVLLLL
                     VALLLPDAAPLLGMFCFGNLLRTSGVVERLNDTAQNALINIVTIFLGLSVGAKLVADK
                     FLQPQTLGILVLGVIAFGIGTASGVIMAKIMNRFSQHKINPLIGSAGVSAVPMAARVS
                     NKVGQEYDKQNFLLMHAMGPNVAGVIGSAIAAGVMLKYISTM"
     gene            complement(5348..7174)
                     /gene="oadA"
                     /locus_tag="PXE44_001390"
     CDS             complement(5348..7174)
                     /gene="oadA"
                     /locus_tag="PXE44_001390"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HDL5588902.1"
                     /translation="MTTQPKKIAITDVVLRDAHQSLFATRLRLDDMLPIASELDNIGY
                     WSLETWGGATFDSCIRFLGEDPWVRLRELKKAMPKTPMQMLLRGQNLLGYRHYADDVV
                     DKFVERSVANGMSVFRVFDALNDPRNMQAALQAVKKHGGHAQGTLSYTTSPVHTLQTW
                     LDVTEQLLEIGVDSLVIKDMSGILTPMAAYELVSEIKKRYDVELHLHCHSTTGMAEMA
                     LLKAIEAGVDGVDTSISSMSGTYGHPATESIVATLQGTPYETGLSIPALEKISAYFRN
                     VRKKYAKFEGQLRGIDSRILVAQVPGGMLTNLESQLKQQNASDKLDLVLQEIPRVRED
                     LGNIPLVTPTSQIVGTQAVMNVLMGERYKTIAKETAGILKGEYGRTPAPVNKALQDRV
                     LEGAEPITDRPADHLPPELEKLVAEVTASAKEKGITLSENMIDDVLIVALFQQVGWKY
                     LENRNNPSAFEPVPSADEAKPAQASAASAPKAQTQSGSAIYTVELEGKAFVVKVSEGG
                     DITNIAPTPATPAPQAVDSAPKSANTLSANAEPVNAPMAGNILKVEVKEGQQVAEGDV
                     LLILEAMKMETQICAAKSGTIQGINVKQGDVVAVNDTLMSIA"
     gene            complement(7214..7468)
                     /locus_tag="PXE44_001391"
     CDS             complement(7214..7468)
                     /locus_tag="PXE44_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit gamma"
                     /protein_id="HDL5588903.1"
                     /translation="MAPAELFAEGINLMVAGMGFVMLFLLVLIYAISFISKIINQYFP
                     EPVSIPASKTVPQSPPDDLERLRPVIAAAIAHHRRINGQH"
     gene            complement(7658..8845)
                     /locus_tag="PXE44_001392"
     CDS             complement(7658..8845)
                     /locus_tag="PXE44_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL5588904.1"
                     /translation="MSNNNRSALLGAAFLMATSAIGPGFLTQTATFTQTLLASFGFVI
                     LLSILLDIGAQLNIWRIVAVSEKKAQDIANAVLPGAGYFLALLIVMGGLAFNIGNVGG
                     AGLGLNILTGLSPEMGAVVSGAIAVGVFLFHEAGKVMDRFAQIMGFVMIALTIFVAVK
                     ANLPIDDAVVHTFVPEKLDVIAIVTLVGGTVGGYITFAGAHRLLDAGIKGKENLKEVS
                     KSSVSAILIASIMRVVLFLAVLGVVSMGVQLDPKNPAATPFAHVAGDVGLMIFGVVIW
                     AASITSVIGAAYTSVSFITSFSPFIEKHKNCFIVAFIVVSTAVLATIGRPAQVLVFVG
                     TLNGLILPISLGLILLAAHKTKIVGDYKHPVWMTVSGAIVVILMAVLSAMTLSKYISG
                     LMA"
     gene            complement(9120..9857)
                     /gene="pxpA"
                     /locus_tag="PXE44_001393"
     CDS             complement(9120..9857)
                     /gene="pxpA"
                     /locus_tag="PXE44_001393"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDL5588905.1"
                     /translation="MNHVDLNVDLAEGCGNDHSLLQLVTSANVCCGLHAGDYNEMRKA
                     INWAKENSVRVGAHPSFPDRENFGRTNMQLPDDELKACLLYQLGAAKALCDAENMPMS
                     YVKPHGALYNMAAKDPHLATLIANTVKQFDPNLKLMGLSGSLMLSIAEQHGLETISEV
                     FADRHYLADGSLVPRTRDDAMVESDDEAISQVLQMVLDGTVPTVDGGNVAIKADSICL
                     HGDGEHAVVFAERIKKELQAKGILVKA"
     gene            complement(9844..10773)
                     /locus_tag="PXE44_001394"
     CDS             complement(9844..10773)
                     /locus_tag="PXE44_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDL5588906.1"
                     /translation="MIYIEIIQGFAHIQDLGRYGYRHLGVSHGGAMDSLALQAGNLLL
                     KNANNAPAIELTLGGMRITFDCDTPFCLTGALVEAELDGTPVFSYYRYTANARQTLTI
                     KRIVLGNYAYLCVAGGFLVPQVLGSASTALKAQFGGFQGRMLKAGDNIATGRRDSRLS
                     LMSIEPIDFTSRIRATASSEYEAFTADSRERFWQQGGQLQNNSNRMGYRFAEKALELT
                     ASLEMLSYAAPSGTVQVPPDGQPIVLMADAQTTGGYPKIACIIQADLGRLAQNPFGST
                     VQFEQVSREQAVEIYQKDQNYLETIRRKANESR"
     gene            complement(10770..11417)
                     /gene="pxpB"
                     /locus_tag="PXE44_001395"
     CDS             complement(10770..11417)
                     /gene="pxpB"
                     /locus_tag="PXE44_001395"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDL5588907.1"
                     /translation="MLTIHHTSEHSLLCLAEPPAELAKQQQIWRVAEQARALDGVLEV
                     VVGMNNFTLFHAIGTDSTRLIQAVQQLWENDQAQAAQSPRLIEIPVHYGGEFGEDLFE
                     VAQFHNTTPEEIIRRHTEPTYTVFMIGFQPGFPYLNGLPQNLHTPRRNVPRTKVPAGS
                     VGIGGRQTGIYPFTSPGGWQLLGRTELPLFDIQHNPPTLLTAGDNVRFVAESITL"
     gene            complement(11511..12335)
                     /locus_tag="PXE44_001396"
     CDS             complement(11511..12335)
                     /locus_tag="PXE44_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDL5588908.1"
                     /translation="MKKLLLFLLFIGNPIFAKSPELMLVEQYQDQDISGWVMSEKLDG
                     VRGFWDGQQLISRQGYPLNPPDYFIQDFPPFAIDGELFSERGKFEEISSIVRSAQPKG
                     WHKLKLQVFDVPNAQGNLIERLATLENYLKQHPTLHIAIIEQIPIKDKAHLQQFYQSV
                     LEQGGEGVIVRNPNAGYIQGRSAQILKIKPVLDEECTVVAHHKGKGKYTDKLGAITCE
                     NQRGRFRIGSGFKDKERENPPPIGSIITYKYRGVTESGKPRFATFWRIRNDQISEN"
     gene            complement(12469..13293)
                     /locus_tag="PXE44_001397"
     CDS             complement(12469..13293)
                     /locus_tag="PXE44_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588909.1"
                     /translation="MEHFIGLGVAGNFAGHLEQAGEEADFAKVKTVEAVQPKAIFPFY
                     VPAENLGDYQFLSTYPLSNTAINFPSDADNLQIEPEVALICEIGYQDQQVVSLTPTHF
                     AAYNDCSIRRPNANKICEKKNWGASSKGLSAIHLPLDNFNKGGTLDNYNIACFHKRNG
                     ELNTYGIDSPAVEYSYFHQKLLDWIVERMNNQPDEGPMNNISEMLKQANYPTKAIISI
                     GATRYTPFGESNLLQVGDVSMVVVYNVKKYSHSQIEEMAKLETFSEDISALIQTVR"
BASE COUNT         3668 a         2993 c         3023 g         3765 t
ORIGIN      
        1 ccgttagaaa ctgatcacga tgtgagtgtt tgggctgctc gttatattgt tgatcgtatc
       61 aatgcattcc aaccaaccaa agaaaaacct tttgtgcttg gcttaccaac cggcggtacg
      121 cctgaaaaaa cctataaaga attaattaaa ttatatcaag ctggtgaagt gagctttaaa
      181 aatgtggtga ctttcaatat ggacgaatac gtcggcttac ctaaggaaca ccctgaaagc
      241 taccacagtt ttatgttcaa gcactttttt gaccacgttg atattccgct tgagaatatc
      301 aatatcctca acggtatggc ggaagacgtg aatgcagaat gcgaacgtta tgaagaaaaa
      361 atccgttttt atggtaaaat ccatctgttt atgggcgggg tcggtgtgga tgggcatatt
      421 gcgtttaatg agcctgcatc ttcgctttct tcccgcactc gaattaaaac gcttaccgaa
      481 gatactcgca ttgcaaactc acgtttcttt aacaatgacg taagccaagt gccgaaattt
      541 gctttaaccg ttggggttgg tacgttgctt gatgccgaag aagtgatgat cttagtgacc
      601 ggccataaca aagccttagc attacaggcg tgtgtggaag gggcggtaaa tcatctttgg
      661 accatcagtt gcttacaatt acatcgccgt ggtatagtgg tgtgtgatga gccggcaacc
      721 caagagctga aagttaaaac agttaagtat ttcaaagagt tagagaaaaa cgtagctcgc
      781 taattctgat tttacaagcg gttaattttg taatattttt tacaaaaacg accgcctgtt
      841 agcatttaaa gagaagagag aaaatgaacc attatgcctt aacaaactgt gtgatttaca
      901 ccggtgaaca agtgctttac caacacgcat tagtggtgaa agaagataaa atcgactcca
      961 ttgttttgga agaaaattta ccgctggatc tgcaaaaaat cgacctaaaa ggagcgaatc
     1021 tttcagccgg ttttattgac ttgcaactga acggctgcgg tggggtgatg tttaatgaag
     1081 aaatcactac ccgcacttta gaaattatgc aggaaaccaa tctgcgttca ggcacaacca
     1141 gctatttacc gacttttatt acctcgcctg atgagggagt gaaacaagcg gtggcggtaa
     1201 tgcgtgagta tttgtcgaaa cacaaaaacc aagcactcgg cttgcatttg gaaggacctt
     1261 atctaagtgt ggcaaagaaa ggagtacacc gtccggaatt tatccgtgaa atcagccctg
     1321 aaatgttaga tttcttatgc gataacggcg atgtaattac gaaaatcacg attgcagcag
     1381 aaaacccgac cgcagaccat atcaaacgct ttaatgatgc aggaattatt gtatctatcg
     1441 ggcattccaa tgccactttt gctcaaacca aacagcgaat tgccgaaggg gcagcgtttg
     1501 ctacgcattt acacaatgca atgtcgccgg tcagcaacgg gcgtgaaggc ggtgtagtag
     1561 gggcggtgtt ggatagcgat atttacaccg gcattatttg cgatggcttg cacgttgaat
     1621 ggggcaacat tcgcattgcc aaaaaagcca aaggcgataa attagtgatt gtcagcgatg
     1681 ctgttgctgc cgcaggaagt gatattgagt cgtttatttt tgtcggtaaa aaagtcacgg
     1741 tgaaagatgg caaatgctac gatgaattcg gagcattggg cggtgcggca atcacgatga
     1801 tcgaatccat tcgcaacgta gtgcaacatt gcgatattag cctgaatgaa gcaatccgaa
     1861 tgagtaccct ttacccggca aaagcgattg gggtagagga aacactcggc tcacttgaag
     1921 ccggcaaaat tgccaacctt gcgattttcg ataatcaatt tgaagtaaaa gcgactgctg
     1981 taaacgggca attacagtgg cattaatttt gctacaaatt ctaagcataa cagcatataa
     2041 tccaaaggca ggcattgtgt ctgcctttta tttataggga gaataacatg caatttaaag
     2101 attacttagc cacttttcca actattgatc atctagccgg cttaaacgtg ataaatagcg
     2161 gtgaagtgat tcaacatatt cctgcgattg aaggtaaatt aggttcacta aaactttaca
     2221 atgcactgtt tgcgaaattt gatgggaaat taaccgctag tgcagcaaaa caagggttgg
     2281 aatggtttgc cgaacacgtt gaagatgcga aaaataatgt gggtaagcac ccgaatattg
     2341 atctattatt tagggtaatt gagcaagatt tatctttaag tttagagccg attgttaaat
     2401 aagattgcac tagtgaaaaa taacaagcgg tcattatctg caaaattttt gcaaataatg
     2461 actgcttgtg tttaagaggc ttatttcacg gtaatgcgtg caaatttgcg tttgccgact
     2521 tggtaaacaa aagtgccttt ttgagcagtt tgtttcacat cttccacttt ttcaccatca
     2581 attttaaccc cgccttgttg ggctgagcga attgcctctg aagtggacgg tactaagccg
     2641 gcttctttca gtagagccgc taggccgatt tcaccctcaa aggtaaattc aggcacttca
     2701 tcaggaattg cccctttttg gaaacggttg ataaactctt gctctgctgc atctgctgcc
     2761 gcttcatcgt ggaaacgagc gataatttct tttgccagta gaattttcac atcacgcggg
     2821 tttttgccat tttcaacatc cgcttttaat tgtgcaattt cgtctaatgg acggaatgag
     2881 agcaggttgt accaatccca cattaaatcg tctgaaatcg acataatttt gccgaacatt
     2941 tcgcttggag cttcagttac accaatgtag ttgccgagag atttcgacat ttttttctcg
     3001 ccgtctaagc cgacaagtag tgggagtgtc attgccactt gtggtttttg accggctgat
     3061 ttttgtaact cacgtccaac taacaagtta aaggtttggt ctgtaccacc taattccacg
     3121 tcagctttca gttctaccga gtcgtgacct tgtagtagcg gataaataaa ttcgtggatt
     3181 gcgatcggtt gttggttcgt gaaacggttt ttgaaatctt cacgttctaa cattcgagca
     3241 acggtgtagt tggaggctaa gcggatcata ccttccgtac ctaacttgcc taaccattcg
     3301 gagttgaata cgatcttggt tttttgtgga tctaaaattt tgaagatctg ctctttatag
     3361 gtttcggcat tgcgtaacac gtcttcacgg ctaagtggcg gacgggtaga gttttttcct
     3421 gatggatcgc ctaccatacc ggtgaaatca ccgattaaga aatagacttc gtgtccgaat
     3481 tgttggaatt gacgaagttt gttcagcaca acagtatgac ctaagtgaat atccggtgca
     3541 gttgggtctg cccctaattt aatacgcaat gggcggtttt cttttaattt ggtgattaaa
     3601 tcttcttctg aataaatatt ttccacgcca cgtttaagtt cggcaaggac tgattcgata
     3661 gcttgagtca tttatcttcc ttttagtctt gaatataaaa aatggatcta ttatagcggt
     3721 atgaattgaa attgataggc tataggcgaa aaaaaacgcc gtattttgtg ggggaatacg
     3781 gcgtaaagag ttggagtgat tacctattat ttttgaagtg atttaatgat aatgattatc
     3841 atctgttaag tcaatacttt ttttgaaaat tttcaataag aattattctc atcaattaag
     3901 taagatggat actacggcaa ttagtgaaga atgtacagcg gaattttcgt attgtaagag
     3961 tgttggtaag cggtattttt agaaaaaacc tccgagagcg gttatttttc ggaggttttt
     4021 tgcaaatgag gatttacatc gttgaaatgt atttcaacat cacccccgct gcaatcgcag
     4081 agccaatcac gccggcaacg tttgggccca ttgcgtgcat taacaagaag ttttgtttat
     4141 cgtactcttg tccgactttg tttgataccc gtgcagccat cggcacggca gaaacacccg
     4201 ctgagccgat aagtgggttg attttgtgct gactaaagcg gttcattatt ttcgccataa
     4261 tcacaccgct tgcagtgccg atcccaaagg caatcacgcc caatactaaa atgcctagtg
     4321 tttgcggttg taagaatttg tctgcgacca attttgcccc caccgataac cctaagaaaa
     4381 tcgtcacgat attgattaac gcattttggg cggtgtcgtt taagcgttct actacgccac
     4441 tggttcgcag taagttgcca aagcagaaca ttcccaataa cggtgcagca tccggcagta
     4501 agagtgccac taataatagc aacacgatag ggaaaataat tttctcacgg ttgcttacat
     4561 tacggagctg aaccattcgg attttacgtt cttgctcggt ggttaaggct ttcataatcg
     4621 gcggctgaat cagtggcact aacgccatat aagaataagc cgctaccgca atcgcaccta
     4681 ataattcagg ggcaagtttg ctggcaagat aaatcgcggt cgggccatct gccccgccga
     4741 taatcccaat cgcagcggct tgtggcaatg tgaagtcgat aatgcctaac cagtttagcc
     4801 ctaatgcacc cagtacggta gcaaaaatcc caaattgagc cgcagcccca agcattaaca
     4861 ttcgagggtt ggcaagtaaa gggccgaaat cagtcatcgc cccgacaccc ataaagataa
     4921 taagcggagc aacgccgtag ccgatagcaa ctttatagaa taatgccaac acaccggcgg
     4981 tatagcccat atcgccagcc attacttcaa gctcattttg aacagaggga gcagccagtg
     5041 cgatggcatc tttaatcgaa tgcagatcaa ggcttgtact atttacctta gcagcaataa
     5101 ttgccagttg ctcggcagaa ccgctgtgga gtaaattgtc taatgccgtc attgccatac
     5161 ctgcttccgg aatgtttgac atcaaaccac caaagccaat aggcagtagt aaaagcggtt
     5221 cgaattttcg ggcgatggca agccaaagca acactaaact gatgacaatc ataatcgctt
     5281 gccccagttc taaatgcatt agccccatac ctttaaaaag tgcaataata ctttccatac
     5341 cggactccta ggcaatactc attaaagtat catttaccgc cacaacatcg ccttgtttga
     5401 cattaatgcc ttgaatcgtg ccggattttg ccgcacaaat ttgggtttcc attttcattg
     5461 cttcaagaat cagtaacaca tcgccttcag caacttgttg cccctctttc acttccactt
     5521 tcaaaatgtt ccctgccatt ggagcattca ccggttcggc attggctgat agcgtatttg
     5581 cagattttgg tgcagaatcg accgcttgcg gagccggtgt tgctggtgtt ggagcgatat
     5641 tggttatatc gccaccttca ctcaccttca ccacaaaggc tttgccttca agctcaacgg
     5701 tataaatggc tgaaccggat tgtgtttggg cttttggtgc agaagcagcg gaggcttgtg
     5761 ccggttttgc ttcatcagca cttggcactg gctcaaaggc agacggatta ttgcggtttt
     5821 ccaaatattt ccagcccact tgttggaaga gtgccacaat caatacatca tcaatcatgt
     5881 tttcagatag cgtaatgcct ttttcttttg cacttgcagt cacttctgcc actaattttt
     5941 ccagttccgg cggaagatga tcggctgggc ggtcggtaat cggctctgct ccctcaagca
     6001 ctctgtcttg caaggctttg tttaccggtg ccggtgtgcg accatattcg ccttttaaaa
     6061 tacctgcagt ttctttggcg atagttttat aacgttcgcc cattaatacg ttcatcaccg
     6121 cttgtgtgcc gacaatttgt gaggttggtg tcaccaacgg aatattgcct aaatcttcac
     6181 gcacacgagg aatttcttgt aaaaccaagt ctaatttatc cgacgcattt tgttgcttga
     6241 gttggctttc gaggttggtc agcatgccac ccggcacttg ggcaaccaaa atacggctat
     6301 ctataccacg gagctgtcct tcaaattttg catatttttt acgcacgtta cggaagtagg
     6361 cagagatttt ctccagagct ggaatgctca agccggtttc atacggcgtg ccttgaagtg
     6421 tggcgacaat agactcggtt gccgggtgac cgtaagtgcc ggacattgat gaaatggagg
     6481 tatcgacacc atcgacaccg gcttcaatcg ctttgagcaa cgccatttca gccataccgg
     6541 tggttgaatg gcaatggaga tgtagctcaa cgtcgtagcg ttttttaatt tcgctgacta
     6601 attcataggc tgccattggg gttaaaatgc ccgacatatc tttgatcacc aacgaatcca
     6661 cgccaatttc caataattgc tcggttacat ccaaccacgt ttgtagggta tgaacagggc
     6721 tggtggtgta actcaaagta ccttgtgcgt gcccgccgtg ttttttgacc gcttgtaatg
     6781 cggcttgcat attgcgaggg tcgttgaggg catcaaatac acggaacaca ctcataccgt
     6841 ttgccaccga acgctcgaca aatttatcta ccacatcgtc ggcataatga cgataaccca
     6901 ataagttttg cccacgtagc agcatttgca ttggggtttt cggcattgct tttttcagct
     6961 cacgtaaacg cacccacgga tcttcgccta aaaaacgaat acaactgtca aaagttgccc
     7021 cgccccaagt ttctaacgac caatagccga tattatccag ctcggaggca atcggtaaca
     7081 tatcgtctaa gcgtaaacga gtcgcaaaaa gggattggtg tgcatcacgc aaaaccacat
     7141 cggtaatcgc aatttttttc ggttgagttg tcataatcaa attccttcta attattttat
     7201 ttttgaaatc ttgttaatgt tgcccgttaa ttcgacgatg atgagcaatc gcagcggcaa
     7261 tcacagggcg taaacgctct aaatcatctg ggggagattg aggcacagtt ttagaggctg
     7321 gaatagaaac aggttcgggg aaatattgat tgataatctt ggaaataaag ctgatggcat
     7381 aaattaaaac cagcaggaac agcataacaa atcccattcc ggctaccatt aaatttatac
     7441 cttccgcaaa gagttctgca ggtgccataa tttatcctta aaatgtagaa atgatgaaga
     7501 aaatgtagaa aaaattcaga tttatttcaa attatttagc ctcaaaatga gtggtagatc
     7561 acaaacttta aacgcaagcg gtcagatttt acaaattttt tacaagagta gttaaaattt
     7621 gcaaaacctg accttaggtt agcttgaagc taagtcgtta tgccattagc cccgaaatat
     7681 attttgaaag ggtcattgca cttagcaccg ccattagaat caccacaatc gcccctgaaa
     7741 cggtcatcca cacagggtgt ttgtagtcgc ccacaatctt ggttttgtga gctgccagca
     7801 gaattaaccc gagggaaatc ggtagaatta agccgttaag cgtccctaca aacaccaaga
     7861 cttgtgccgg tctgccgatg gttgccagca cggcggttga aaccacaata aaggcgacaa
     7921 tgaaacagtt tttgtgtttt tcgataaatg gcgagaatga agtaatgaag gaaaccgagg
     7981 tataagccgc cccaatcaca gaggtaatgg aggctgccca aatcaccacg ccgaagatca
     8041 tcaaaccgac atcgccggca acgtgggcaa atggggtagc cgcagggttt ttcggatcaa
     8101 gttgcacgcc cattgacacc acgcccagca ccgctaaaaa cagtaccaca cgcataattg
     8161 aggcgattaa aatggctgac accgagcttt tgctcacctc ttttaaattc tctttgcctt
     8221 taatgcctgc atcaagcaaa cggtgcgcac cagcaaaggt gatgtatcca ccaacagttc
     8281 cgcccactag cgtgacaatc gcaatcacgt ctaatttttc cggcacaaag gtatgcacca
     8341 ccgcatcgtc gattggcaga ttggctttta cggcaacgaa gatggttaaa gcaatcatca
     8401 caaagcccat aatttgtgca aatctgtcca tcactttacc agcttcatgg aacaggaaca
     8461 cgcccacggc aatcgcaccg ctgaccaccg cacccatttc aggggaaagt ccggtgagaa
     8521 tgtttagccc taaacctgca ccgcccacgt tgccgatatt aaaggctaaa ccgcccatca
     8581 cgataagcag agccagaaag taacctgccc ccggcaatac ggcattggcg atgtcttgag
     8641 ctttcttttc cgatactgct acaattcgcc aaatattcag ttgagcaccg atatcaagca
     8701 agatcgagag taagatcaca aagccgaagc tggcaagtag ggtttgggtg aaggtggctg
     8761 tttgggttaa gaaccccggc ccgatggcgg aggtcgccat taaaaatgct gccccgagta
     8821 gggcgctacg gttgttgttt gacattgtgt tggttccttt tttgttgtta atgagtcatt
     8881 attcgtggat ttatcatggt aatattcccc tctttagcaa agaggggtta ggggagattt
     8941 ggcagtagaa ctcatcgaag tgagagagtt aataatgctc aatttaacct cttgaaatta
     9001 cttattttct ccgtaaatcg cttctgtcaa atctccccca gcccctcttt tctaaagagg
     9061 ggagttcgat ctccaagagt tttatttctg gttttccgtc cattttgact aaaacaggat
     9121 caggctttca ccaaaatccc ctttgcctgt aactcttttt taattcgctc ggcaaatacc
     9181 acggcgtgtt cgccgtcacc gtgtaagcaa atgctgtcgg ctttaatcgc cacattacca
     9241 ccatcaacgg ttggtacagt gccgtctaac accatttgca agacttggct gatcgcttca
     9301 tcatcgcttt ctaccattgc atcatcacgg gtgcgaggca cgagcgagcc gtcagctaaa
     9361 tagtggcggt cggcaaagac ttcggaaatg gtttccaaac cgtgctgctc ggcaatgctc
     9421 aacatcaggc tgccggataa gcccatcagt ttgaggttgg gatcgaactg tttgaccgta
     9481 ttggcgataa gggtggcaag gtgagggtct ttggcggcca tattgtataa agcgccgtgc
     9541 ggtttaacgt agctcatcgg catattttcc gcatcacata aggctttggc ggctcccagt
     9601 tggtagagca gacaggcttt aagttcatca tcgggcagtt gcatattggt acgcccaaag
     9661 ttttccctat ctggaaaact cgggtgagcg ccaactcgca cgctgttttc cttcgcccag
     9721 ttaatcgctt tacgcatttc attgtaatca ccggcgtgca agccacagca gacattcgcc
     9781 gaagtaacga gctgtaataa tgaatgatcg ttgccacaac cttcggcaag atcgacattt
     9841 aaatcaacgt gattcattcg cttttctccg aattgtttcc aaataatttt gatctttttg
     9901 ataaatttcg accgcttgtt cacggcttac ttgctcaaat tgcaccgttg agccgaacgg
     9961 attttgtgcc aaccgcccga gatcggcctg aataatgcag gcaattttcg gataaccacc
    10021 ggtggtttgg gcatctgcca tcagcacaat cggctggccg tccggcggca cttgcaccgt
    10081 accactcggg gctgcgtagg agagcatttc aagggaagcg gtcaattcca acgctttttc
    10141 tgcaaaacgg tagcccatac ggttgctgtt gttttgcagt tgcccccctt gttgccaaaa
    10201 gcgttcacgg ctatctgcgg taaaggcttc atattcgctc gaagcggttg cccgaatgcg
    10261 tgaggtgaaa tcaatcggct caatcgacat caaagaaagt cggctgtctc tgcggcctgt
    10321 ggcgatgtta tcgccggctt ttaacattct gccttgaaaa ccgccaaatt gagctttgag
    10381 agcggtactt gccgaaccta acacctgcgg caccagaaaa ccgcctgcca cgcataaata
    10441 ggcgtaattg ccgagaacaa tccgttttat cgtcagcgtt tgacgggcgt tggcagtgta
    10501 gcggtaatag gaaaaaaccg gcgtgccgtc cagctccgcc tccaccaatg caccggtgag
    10561 gcaaaatggc gtgtcgcaat caaaggtaat ccgcattccg ccaagggtaa gttcaatcgc
    10621 aggtgcatta tttgcatttt ttaacaataa attacccgct tgtaaggcaa ggctgtccat
    10681 cgcaccgccg tggctcacgc ctaaatggcg ataaccataa cgccctaaat cttgaatgtg
    10741 ggcaaaaccc tgtattattt cgatataaat catagcgtta tactctccgc cacaaatcta
    10801 acattatcgc cggctgtaag tagcgttggc ggattgtgtt gaatatcaaa caacggtaac
    10861 tcggttcgtc ctaacagttg ccagccacct ggtgaggtaa agggataaat gcctgtttgc
    10921 ctgccgccga tgccgaccga accggcaggg actttggtgc gaggcacgtt tcggcgtggg
    10981 gtgtgtaaat tttgcggtaa gccgttgagg taaggaaagc cgggttgaaa gccgatcata
    11041 aacacggtgt aagtcggttc ggtatgacgg cggataatct cttcgggagt ggtattgtgg
    11101 aattgggcga cttcaaacag atcttcgcca aattcaccgc cgtaatggac agggatttca
    11161 atcaggcgtg gggattgtgc ggcttgggct tggtcgtttt cccagagttg ttgaaccgct
    11221 tggataaggc gtgtgctatc ggtgccaatg gcgtgaaata gggtaaaatt gttcattcct
    11281 accaccactt ccaacacgcc atctaaggct cttgcctgtt ctgccacccg ccaaatctgt
    11341 tgctgtttgg caagctctgc aggcggttcg gctaagcaga gcagggaatg ttcgctggta
    11401 tgatgaatcg tcagcataat tgccctcaaa ttgttacaaa aaggtaacaa ctttttacgc
    11461 tgatatatcc agactgtcaa ttttattttg ttgaaaatgt gaaatagctc ttaattttca
    11521 gaaatttgat catttcttat tcgccaaaac gttgcaaatc tgggcttgcc tgattctgtg
    11581 acacctcgat atttataggt aatgattgag ccaatcggtg gcggattttc tcgttcttta
    11641 tctttaaaac cgctgccaat ccgaaaacgt ccacgttgat tttcacaagt gattgccccg
    11701 agtttatcgg tatatttccc tttgccttta tgatgggcaa ccacagtgca ttcctcatct
    11761 aaaaccggtt tgattttcaa aatttgggca gaacgacctt gaatatagcc tgcattcggg
    11821 ttgcgaacga taaccccttc gccaccttgt tccagcacag attggtaaaa ttgttgaagg
    11881 tgggctttat ctttaatcgg aatttgctca ataatagcga tatggagcgt tggatgttgc
    11941 ttcagataat tctctaaggt cgccagccgt tcaatcaaat tcccttgtgc atttggcaca
    12001 tcgaacacct gtaatttcag cttgtgccaa cctttgggct gagcggaacg cacaatgctt
    12061 gaaatttcct caaatttgcc ccgttcgcta aacagttcgc cgtcaatcgc aaaaggaggg
    12121 aaatcttgaa taaaatagtc gggtggattg agcggataac cttggcggct gatgagctgt
    12181 tgaccgtccc aaaagcctcg cacgccatcg agtttttctg acattaccca gccactgatg
    12241 tcttgatctt gatattgctc aacgagcatt aactcgggcg atttggcaaa aatagggtta
    12301 ccaatgaaga gtaggaataa gagcagtttt ttcatagtct ctccgaaata gtgcctccga
    12361 atatgcaagg cactataaag gaaagcgtgc aaacagtcag aaatgaatct caatttttcg
    12421 acgcagatcg caaaattttc cgaaaaactg atcgcttgag tgagaaattt aacgtactgt
    12481 ttgaattaaa gcagaaatat cctcgctaaa ggtttccaac ttcgccattt cttcaatttg
    12541 gctatgagaa tatttcttca cattgtaaac gaccaccata cttacatcac ctacttgcaa
    12601 taaattactt tcgccaaaag gcgtgtagcg tgttgcccca atgctgataa tcgctttagt
    12661 tgggtagttt gcctgtttta acatttcgga aatgttgttc atcggacctt catcgggctg
    12721 attattcatt ctctctacaa tccaatcgag caatttttga tggaaatagc tgtactccac
    12781 cgcagggctg tcgatgccgt aggtattcag ctcgccatta cgtttatgga aacaagcgat
    12841 gttgtaatta tccagcgtgc cgcctttgtt gaagttatct aacggaagat gaattgccga
    12901 aagcccttta gacgaagccc cccagttttt cttttcacag attttattcg cattcggacg
    12961 gcggatagag cagtcattgt aagccgcaaa atgggttggc gtgagcgaaa ccacttgctg
    13021 atcttggtag ccaatttcac aaattaacgc cacttcaggc tcaatttgca gattatcagc
    13081 atcactcggg aaattaatcg cagtatttga aagcggatag gtggataaaa actgataatc
    13141 ccccaaattt tccgccggca cataaaacgg gaaaatcgcc tttggctgca ctgcttccac
    13201 tgttttcact ttcgcaaaat ccgcctcctc tccggcttgt tctaaatgtc cagcaaaatt
    13261 gccggccacg cctaaaccga taaagtgttc cataatctac tccttaattg gtaaaagttg
    13321 gataaaattt taccgcttgt aaccaaaaat tgcgaatgtt tattttgatc tgattgatag
    13381 aaaattattg aattgagatt aaatttagcc atattttgaa cataaaatta gcgctttgtt
    13441 ataaaaaca
//
LOCUS       DANLZC010000062        13379 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.062, whole genome shotgun sequence.
ACCESSION   DANLZC010000062 DANLZC010000000
VERSION     DANLZC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13379
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.062"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            78..2534
                     /gene="thrA"
                     /locus_tag="PXE44_001398"
     CDS             78..2534
                     /gene="thrA"
                     /locus_tag="PXE44_001398"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDL5588910.1"
                     /translation="MRVLKFGGTSLANPERFMQAAEIIEKAHLADQAAGVLSAPAKIT
                     NHLVAIVDKAIAGESFQSNLSEATEIFHNIINGLYATNNHFAKEALLALVEAELNQIQ
                     QVAAEAAQTKSLSDKIAATIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSY
                     LESSVDIAESTKRVDAGSIPKKNIVLMAGFTAGNEQGELVLLGRNGSDYSAACLAACL
                     KADVCEIWTDVDGVYTCDPRLVPDAVCLETMSYQEAMELSYFGAKVIHPRTIGPLVPL
                     NIPCLIKNTGNPDGKGTLIDGNVANDNLKVKGITNLDNVAMFNVSGSGMQGMVGMAAS
                     VFSTMSKAGISVILITQSSSEYSISFCVPAKSAEKALAALNTEFAQELSENLLDPIDI
                     IKDLSIVSVVGDGMRTAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPLNKAIE
                     AVKATHKSLFNSKRVVDMFLVGVGGVGSELIEQVKQQKEFLAKKDIEIRVCALANSDK
                     MLLNENGLNLDNWKEDLEKAIQPSDFDVLLSFIKFHHVVNPVFVDCTSAQSVANLYAR
                     ALKEGFHVVTPNKKANTSGYDYYLEMRENARQSQHKFLYETNVGAGLPVIENLQNLLA
                     AGDEVEKFEGILSGSLSFIFGKLEEGLTLSQATLIAKEKGFTEPDPRDDLSGADVARK
                     LLILVRETGLPLEFEDIEIEGVLPKGFSEGMSRDEFLQALPSLDEEFSKRVEAAKAEG
                     KVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFLTRYYNPIPLLLRGYGAGN
                     SVTAAGIFADILRTLQGGVN"
     gene            2534..3481
                     /gene="thrB"
                     /locus_tag="PXE44_001399"
     CDS             2534..3481
                     /gene="thrB"
                     /locus_tag="PXE44_001399"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDL5588911.1"
                     /translation="MALRIYAPASSANISVGFDSLGAAISPIDGSLLGDVVQIEESEQ
                     QFELESAGYFVRKLPKEPQKNIVYQAYVLFSERLKLRNGSVKNLRLTLEKNMPIGSGL
                     GSSACSIVAALVALNKFHDEPFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGLQLM
                     TQSLGNICQPLPFFDEWYWVLAYPGVEVSTAEARAILPKSYTRQDMIQQARYLGSFVH
                     ACHTHQDILAATMMKDLIAEPYREDLLPNFPAVRQGCKDLGALAVGISGSGPTMFAIA
                     PDLEHAQRLVSYLEKEYLQNNEGFIHICKVDNQGARELK"
     gene            complement(3596..4972)
                     /gene="cysS"
                     /locus_tag="PXE44_001400"
     CDS             complement(3596..4972)
                     /gene="cysS"
                     /locus_tag="PXE44_001400"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL5588912.1"
                     /translation="MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTF
                     VSFDVIARYLRYSGYNLRYVRNITDVDDKIIKRALENNETCDELVDRMIAEMHKDFDA
                     LNILRPDVEPRATKHIPEIIAMVEKLIHNGHAYVAADGDVMFDVESFQKYGALSRQNL
                     EQLQAGARVEIKSIKKNPMDFVLWKMSKAGEPSWESPWGNGRPGWHIECSAMNSKELG
                     EHFDIHGGGSDLMFPHHENEIAQSCCAHGGDYVNYWLHTGMLTIDKEKMSKSLGNFFS
                     IRHMLGLYDAESLRYFFLTAHYRSLLDYSIDNLDLARSALERLYTALRGCDVSGEAIG
                     EHYVEAFKTAMDDDFNTPGALAVLFEIARELNKLKTEDMAKANGLAVRLKELAGVLGL
                     LEQDPDAFLKGDANDDETAEIEALIKQRNEAKAAKNWAVADEVRDKLKAINIVLEDTP
                     NGTIWRKA"
     gene            5128..5637
                     /locus_tag="PXE44_001401"
     CDS             5128..5637
                     /locus_tag="PXE44_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5588913.1"
                     /translation="MITLHTNFGDIKIELNFEKAPITAKNFEDYAKEGFYNGTIFHRV
                     IDGFMIQGGGMTAGMAEKPTKDPIQNEANNGLSNKRGTIAMARTSDPHSASSQFFINV
                     ADNTFLDYRSKEMFGRSVVQDWGYAVFGEVVEGMDVVDKIKGVKTGNKGFHQDVPVED
                     VVIESVSVA"
     gene            complement(5729..6658)
                     /locus_tag="PXE44_001402"
     CDS             complement(5729..6658)
                     /locus_tag="PXE44_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL5588914.1"
                     /translation="MEIALLLADKILQLTLMVLLGYLIVKTKLLKSEHSYPISVIGLY
                     IISPAMLITAFQVDYTTEILNGLYLAFLISVLLNGLLILLGWLLKRALKLDNIEQATA
                     IYSNSGNLIIPIVASLFGNEWVIYSTAFIVVQNVLFWSHLRLLICGKGNVPFRKMIFN
                     INIIAIVIGMMLFLLHIKLPATLANTLSTLGHMLGPLAMLVAGMLIAAIPFKEIITDK
                     RIYLVAFLRLIFIPLILLAVIKLGGFASWIENGKTVVMITFLATISPSAATATQMAVA
                     YGQDAKKASAIYGVTTLLCVFTMPLIIWLYQFL"
     gene            complement(6661..7455)
                     /gene="fdhD"
                     /locus_tag="PXE44_001403"
     CDS             complement(6661..7455)
                     /gene="fdhD"
                     /locus_tag="PXE44_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDL5588915.1"
                     /translation="MNAKKLSLSTNSIKQKDDEENLISEVPVALVYNGISHTVMMATP
                     ANLEDFALGFSLAEGILNAPSELYGLDVIEQENGVEIQLEISSRQFVALKDKRRSMAG
                     RTGCGICGVEQLEQVNQSCKIVKKNNRLQNVNPLILEDCLLQLENAQFLSKETGASHA
                     AAFFTPDGKLLAIREDVGRHVALDKLIGWYAKADFPIGFVFVTSRASFEMVQKCLAVG
                     IEMLCAISATTEMAVNMARENQFTLLAFARKGKATIYAGEERLALN"
     gene            7709..10780
                     /gene="fdnG"
                     /locus_tag="PXE44_001404"
     CDS             7709..10780
                     /gene="fdnG"
                     /locus_tag="PXE44_001404"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:8294..8296,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDL5588916.1"
                     /translation="MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAK
                     ETRQTCTYCAVGCGMIMYSLGGEAMNSKGKLIHIEGDSDHPVSRGALCPKGAGALDYV
                     NSDRRVHYPEVREAGSSEWKRISWHEAIERIARHMKNDRDENFVTHNEAGLEVNRWMT
                     AGFLAGSACSNETGIVTQKFIRSLGMIFTDNQASIUHGPTVASLAPSFGRGAMTNHWV
                     DIKNSDLVIVMGGNAAEAHPVGFRWAVEAKKQNGAKLMVVDPRFNRSAAVADIYIPLR
                     PGTDIAFLLGAVNYLLKNDKIQHEYVKHYTNATFLLNEGFKFEDGLFTGYDEATRTYK
                     DKSTWAYQLDEQGQPKRDMTMQDPRCVINMLKDHVSRYTPEMVERITGTPQKDFLIFC
                     EEIAKTSAPDKAATFLYALGWTQHTVGAQNIRSMAIIQLLCGNIGVSGGGVNALRGHS
                     NVQGITDLGLFPHMLPGYIRLPTEADATLESYLNAIAPKTLVAGQVNYGQNNPKFAVS
                     MLKTFYGDKATKENEFGYHYLPKYPKGGTDHFRFIEQMYQGKVNGYFCQGMNPIASYP
                     NSQKLIKALSNLKYLVIFDPLITDTSEFWKNHGEFNNVNPSEIQTEVFRLPTTCFAEE
                     DGSIANSGRWLQWHWKAADAPHEAKPDVDILSEIRAELIHLYHKEGGKAPLEPLEAMT
                     WNYMNPLEPKPEEIAKENNGYALEDLKDADGNIIVKKGELLSSFTQMRDDGTTSGGCW
                     IYCGQWTPKGNQMANRDNSDPSGLGNTLGWAFSWPVNRRILYNRASADLSGKPWNPKR
                     QLVKWNGKNWNYVDVADYGTAPPNSPVTPFIMQPEGVSGLFVRERMQDGPFPEHYEPF
                     ETPIGTNPLHPNVISSPVARILPSDKEDLGTSDEFPYVGTTYRLTEHFHYWTKNALIN
                     VIAQPEQFVEIGEKLAEEKGIKNGDKVRVWSKRGEIKAVAVVTKRIRSLVSDGKPIHT
                     IGVPIHWGFAATTGAKKGYFANNLTVRTGEPNSNTPESKCMLLNIEKIVGA"
     gene            10783..11697
                     /gene="fdxH"
                     /locus_tag="PXE44_001405"
     CDS             10783..11697
                     /gene="fdxH"
                     /locus_tag="PXE44_001405"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HDL5588917.1"
                     /translation="MSTVQTQNIIKISATSALTPAPQARSHQVEVAKLIDVTTCIGCK
                     ACQVGCSEWNDIRSDVNANCVGVYDNPADLNAKAWTVMRFNEVEENDRLEWLIRKDGC
                     MHCAEPGCLKSCPSPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPDDNRVY
                     KCTLCVDRVNVGQEPACVKTCPTGAIRFGSKEEMKFYAEQRIADLKSRGYENAGLYDP
                     EGVGGTHVMYVLHHADKPELYSGLPKDPKIDTTVTLWKDVLKPVAAVALGGLALAEVA
                     HYITVGPNVEEDLEEHHHSENAEGGKHE"
     gene            11690..12367
                     /locus_tag="PXE44_001406"
     CDS             11690..12367
                     /locus_tag="PXE44_001406"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HDL5588918.1"
                     /translation="MSKKFEITNDTKIVRHKPLARVSHWFLVISFFMTMFTGVAFFFP
                     DFAWLTEILGTPQIARAVHPFTGIIMFIAFIIMALIYWHHNIPEKNDIRWAKGIVEVL
                     KGNEHKVSDNGKYNLGQKLLFWTLILAMFTLLATGIIMWRQFFSHYFSIPMLRIAILL
                     HSASAFMLFTGILVHMYMAFWVKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELERQ
                     VAEQNNK"
     gene            12423..13340
                     /gene="fdhE"
                     /locus_tag="PXE44_001407"
     CDS             12423..13340
                     /gene="fdhE"
                     /locus_tag="PXE44_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDL5588919.1"
                     /translation="MSIKILPQDDIKQAASSFQQPELLFANPKNLYLRRAKRLRELAK
                     QNPFGEYMEFSANLVDIQLALLESQPIADYSQKITACVERTQGETPLNAQNFQRTDEW
                     RTLLLAIIEKFKPYANDTVLATIEVLEKAAKSELDTLADNLLNERYELVGADKAVFIW
                     AALSLYWVQLAQQLPRNSRAEIGHKHLCPVCNSAPVSSVIHFGEAQGLRYLHCSLCES
                     EWHVVRTKCTNCDETGKLDYWSLDTVEAAVKAESCGDCHSYLKVLYQDKDVNVEPVAD
                     DLATLFLDSEMEQKGLSRSGINPFLFQVE"
BASE COUNT         3931 a         2858 c         2939 g         3651 t
ORIGIN      
        1 tgaaagaata aaccgaaaac ccgctacaag cgggtttttt tgtataaaaa tttacaaata
       61 atttatcagg agtaactatg cgagttctta aatttggagg cacttctctt gccaaccctg
      121 aacgctttat gcaagcggca gagattatcg agaaagctca tttggcagat caagctgccg
      181 gcgtattatc cgccccagcc aaaattacca atcatcttgt cgctattgta gataaagcga
      241 ttgccggtga atcatttcaa tctaaccttt ctgaagctac cgagattttc cacaacatca
      301 ttaacggctt atatgctaca aataatcact ttgctaaaga agcactgtta gccttagtgg
      361 aagcggaatt aaaccaaatt cagcaagtgg ctgcagaagc agctcaaact aaatcgttgt
      421 cagataaaat tgctgccact attcactgcc gtggtgaaaa actctctatt gcgatgatgc
      481 aagcgtggtt cgaggcaaaa ggctatgaag tcactcgtat cgaccctgtt gaaaaattat
      541 tggctcacgg cagctatttg gaatcttcgg tcgatattgc cgaatccacc aagcgagttg
      601 atgcaggttc aattccaaag aaaaatatcg tgctaatggc tggttttacc gcaggtaatg
      661 agcaaggcga actggtgtta ttagggcgta acggttcaga ttattctgcc gcttgtttgg
      721 cagcgtgttt aaaagccgat gtttgtgaaa tctggacgga cgtagatggc gtttacacct
      781 gcgaccctcg tttggtgcct gatgcagtat gcttagaaac aatgagctat caagaggcaa
      841 tggagctttc ctactttggg gcgaaagtga tccaccctcg caccatcggg ccattagtgc
      901 cgctcaatat tccttgttta atcaaaaata ccggcaatcc ggacggaaaa ggcacattaa
      961 ttgatggcaa tgtggcaaac gataatctca aagtaaaagg gattaccaac cttgataacg
     1021 tggcgatgtt caacgtttca ggttcaggaa tgcagggaat ggtgggcatg gctgccagtg
     1081 tgttctcgac gatgtcaaaa gccggtattt cggtgatttt aattacccaa tcatcatcag
     1141 aatatagcat cagcttctgc gtgccggcaa aatcggcaga aaaagcgtta gcggctctaa
     1201 atactgaatt tgcacaagag ttatctgaaa acttacttga cccgattgat attattaaag
     1261 atttatccat tgtttcggta gtgggtgatg gtatgcgtac cgccaaagga attgcggctc
     1321 gcttcttctc tgccctagcc caagccaaca tcagtattgt ggcaattgca cagggttcat
     1381 ctgagcgttc catttctgct gtcgtgccgt taaacaaagc gattgaagca gtaaaagcaa
     1441 ctcacaaatc gctatttaac agtaaacgtg tggtggatat gtttttagtg ggtgtaggcg
     1501 gtgtgggtag cgaactcatc gagcaagtca aacaacaaaa ggagtttttg gcaaagaagg
     1561 atattgagat ccgtgtgtgt gctttagcta attcagataa aatgttactc aacgaaaacg
     1621 gcttaaattt agacaactgg aaagaagatt tagaaaaagc aattcagccg tcagactttg
     1681 atgtattgct ttctttcatc aaattccacc acgtggtaaa cccagtattt gtagattgca
     1741 cttcggcaca atcggtggcg aacctgtacg cacgagcctt aaaagagggc ttccacgtgg
     1801 taacaccaaa caaaaaagcc aatacatcag gttacgatta ctacttagaa atgcgtgaaa
     1861 acgcccgcca aagccagcac aaattcctct acgaaactaa tgtaggggca ggtttaccgg
     1921 taattgaaaa cttacaaaac ctattagctg ccggtgacga agtagagaaa tttgaaggga
     1981 ttttatccgg ctcgctctct ttcattttcg gcaaattaga agaaggttta accctttcgc
     2041 aagcaacttt aatcgccaaa gaaaaaggct ttaccgagcc agatccacga gatgatttat
     2101 ccggtgcaga tgttgctcgt aaactcttaa ttcttgttcg tgaaaccggc ttaccgcttg
     2161 aatttgaaga tattgaaatt gaaggcgtat tgccaaaagg cttctcggaa ggaatgagtc
     2221 gtgatgagtt tttacaagct ctaccgtctt tagacgaaga attcagcaag cgtgtagaag
     2281 ctgccaaggc tgaaggcaaa gtgttgcgtt atgtgggctc gatcacaggt gataaatgcc
     2341 gagttgccat tgaagccgtg gacgaaaacc acccacttta caaagttaaa gacggcgaaa
     2401 atgcccttgc ttttctcaca cgttactaca accctattcc attattattg cgtgggtatg
     2461 gtgcgggcaa tagcgtaacc gctgccggta tctttgcaga tattctgcgt actttacaag
     2521 gaggagtgaa ctaatggcgt tacgaattta tgccccagct tcaagtgcaa atattagtgt
     2581 gggttttgac tcattaggcg ctgcaatctc cccgattgac ggctctttgc tcggcgatgt
     2641 agttcaaatt gaagagagtg aacaacaatt tgagctagaa agtgcaggtt attttgtacg
     2701 taaattgccc aaagaaccac aaaaaaacat tgtctatcaa gcctatgtgc tatttagcga
     2761 acgcttgaaa ttacgtaacg gcagtgtaaa aaatttacgc ttaacacttg aaaaaaatat
     2821 gccgattggc tcagggctag gctcaagtgc ctgctcgatt gttgcagcat tagtggcact
     2881 caataaattc cacgatgaac cgttctccaa aatggaatta ctggaaatga tgggcgaatt
     2941 ggaaggcaga atttcaggct caatccacta cgacaacgtt gccccttgct atttaggtgg
     3001 cctgcaatta atgacacaat cgctcggcaa tatttgccaa cctctgccat tctttgatga
     3061 atggtattgg gtgcttgcct acccgggcgt ggaagtatca accgccgaag cccgtgctat
     3121 tttgccgaaa agctatactc gtcaagatat gattcagcaa gcccgctact taggatcttt
     3181 cgtccatgct tgccatacac atcaagatat cttggctgct acaatgatga aagacctaat
     3241 tgctgagcct taccgtgaag atttactgcc gaactttccg gctgttcgtc aaggctgtaa
     3301 agacttaggt gcgttagcgg ttggtatttc aggttccggt ccgacaatgt ttgcgatagc
     3361 ccctgatctc gaacacgctc aacgtttagt gagctattta gagaaagaat atctgcaaaa
     3421 caacgaaggc tttattcata tctgtaaagt cgataatcaa ggagcaagag agctgaaata
     3481 gccctctcga acaaccatat cactaaataa cttaaaggac tgactccaat accacttgga
     3541 gcagcccttt ttgattaatt ttttgaaaag tctatcactt ataaacaatc gaaggctatg
     3601 ccttacgcca aattgtgccg tttggggtgt cttcgaggac aatatttatc gctttgagct
     3661 tatctcgcac ttcatcggca accgcccagt ttttagccgc tttggcttcg ttgcgttgtt
     3721 tgattaaggc ttcgatttct gccgtttcat cgtcattcgc atcacctttt aaaaacgcat
     3781 cggggtcttg ttccagtaag cctagcacgc cggctaactc ttttaaacga acggctaaac
     3841 cgttggcttt tgccatatct tcggttttga gtttgttcaa ctcacgggca atttcaaata
     3901 acaccgctaa tgcccccggg gtgttgaaat catcgtccat tgcggtttta aaggcttcta
     3961 catagtgttc tccgatggct tcgcccgaca catcacaacc acgcagagcg gtatataaac
     4021 gctcaagagc cgaacgggct aaatcaaggt tgtcaatgct gtaatctaat aacgaacggt
     4081 aatgagcggt taagaagaaa taacgtaacg attcggcatc gtataagcct aacatatggc
     4141 gaatgctgaa gaaattgccc aaagatttcg acattttttc tttatcaatg gtaagcattc
     4201 cagtgtgtaa ccaataattg acataatcgc caccgtgagc acagcaagat tgtgcaattt
     4261 cgttttcgtg atgcgggaac attaaatccg agccaccgcc gtgaatatca aaatgttcgc
     4321 ccaactcttt gctattcatt gccgaacatt caatatgcca gcccggtctg ccattgcccc
     4381 acgggctttc ccagcttggc tcaccggctt tcgacatttt ccacaagaca aaatccatcg
     4441 ggtttttctt gatggattta atttcaaccc ttgcaccggc ttgtagttgc tctaaatttt
     4501 ggcgggataa tgcaccatat ttctggaacg attccacatc aaacatcaca tcgccatcgg
     4561 ctgcgacata agcgtgtccg ttgtgaatca gtttttctac catcgcaata atttcaggaa
     4621 tatgcttggt ggctcgtggc tcaacgtccg ggcgaagaat gtttaacgca tcaaaatctt
     4681 tgtgcatttc agcaatcatt ctatccacta attcatcgca agtttcgtta ttttctaacg
     4741 cacgtttgat gattttgtcg tccacatcgg taatgttacg cacatagcgt aaattgtagc
     4801 ctgaataacg taaataacgg gcaatcacat cgaaagagac aaaagtgcga ccgtgcccaa
     4861 aatgacaaag atcgtaaacc gtgacgccac agacgtacat tcccacctga tttgggttaa
     4921 tcggtttaaa ttcttctttt tcacgtttta aggtgttata aattttgagc ataaagtcct
     4981 cttgtgtaac attcagtaac aaatggggct aagtatagcg aataagcggt ctgattttga
     5041 gatttttttg caatttggcg taaacttttt aataatagcg agcgaaattt attttccatt
     5101 ccatttttat ttataaagga acaaagaatg attacattac ataccaattt cggtgacatt
     5161 aaaatcgaat taaactttga aaaagccccg attaccgcta aaaactttga agattacgct
     5221 aaagaagggt tttataacgg cacgattttc caccgtgtga ttgacggctt tatgattcaa
     5281 ggtggcggaa tgacggcagg tatggctgaa aaaccgacaa aagatccgat tcaaaacgaa
     5341 gcgaacaacg gtttaagcaa caagcgtggc acaatcgcaa tggctcgcac ttctgatccg
     5401 cattctgctt cgtcacagtt tttcattaat gtggcggata atactttctt agattatcgc
     5461 tcaaaagaga tgttcggcag aagtgtagtg caagattggg gctatgcagt atttggcgaa
     5521 gtggttgaag gtatggacgt ggtcgataaa atcaaaggcg taaaaaccgg caataaaggc
     5581 ttccaccaag acgtgccggt tgaagatgtg gtgattgagt ctgtaagcgt tgcctaattc
     5641 cgcaaattag cctacaagcg gttgattttt cacaattttt tgcatttctc agcgttgcaa
     5701 aaaagtagca gaaatcgact gcttgttttt atagaaattg atacagccaa ataatcagcg
     5761 gcattgtaaa cacacaaagt aaagtggtta cgccataaat cgcactggct tttttggcat
     5821 cttgcccgta agccaccgcc atttgggtcg ccgttgcagc agaagggctg atggtggcta
     5881 agaatgtaat cattaccacc gtttttccat tctctatcca gctcgcaaaa ccgccgagtt
     5941 taatcaccgc taacaaaatc agcggaataa aaatcaagcg gagaaaagcc accaagtaaa
     6001 tgcgtttatc ggtaataatc tctttaaatg gaattgctgc aattaacatc cctgccacaa
     6061 gcattgctaa aggccctaac atatgcccta aagttgagag cgtgtttgca agcgtagcgg
     6121 gcagtttgat atgtagcaaa aacaacatca taccaatcac aatcgcaata atgttgatat
     6181 taaagatcat ctttctaaac ggcacattgc ctttgccaca aatcagcagc cgtaagtgag
     6241 accaaaacaa cacattttgc accacaataa aagccgttga ataaatcacc cattcattgc
     6301 cgaacaggga ggcaacaatc ggaataatta agttgccgga gttggaataa atagcggtgg
     6361 cttgttctat gttgtctaat ttcaacgctc ttttgagcag ccaaccaagc agaattaaca
     6421 agccgtttaa taagacggag attaaaaatg ccagatacaa gccgtttagt atttcggtgg
     6481 tgtaatctac ttgaaaagca gtaatcagca ttgccgggct gataatgtaa agtccgataa
     6541 ccgaaatcgg ataactgtgc tccgatttga gtaatttagt tttaacgatg aggtagccga
     6601 gcagcaccat cagggttaat tgtagaattt tatcggctaa taaaagggct atttccatta
     6661 tcaattaaga gcgagtctct cttctcctgc ataaatggtc gctttgcctt tgcgggcaaa
     6721 agcaagtaag gtaaattgat tttcacgagc catattcact gccatttcag tggtcgctga
     6781 gatggcacac aacatttcaa tgccaacggc taagcatttc tgcaccattt caaagctggc
     6841 acgactggtt acaaacacaa atccaatcgg aaaatcagct ttagcatacc aaccgattaa
     6901 tttatccaac gccacgtgtc tgcccacatc ttcacgaatg gcaagcagtt ttccatccgg
     6961 ggtaaaaaat gcagcggcgt ggcttgcacc ggtttctttc gataagaatt gagcattttc
     7021 tagctgtaat aaacaatcct ctaaaatcag tggattgacg ttctgcaagc ggttattttt
     7081 cttaacaatt ttgcaacttt gattaacctg ctctaattgc tctacaccgc aaataccgca
     7141 acctgttctg cctgccattg agcggcgttt atcttttaat gcaacaaatt ggcggctcga
     7201 tatttcaagc tgaatttcta ctccattttc ttgttcaatt acatctaaac catataattc
     7261 cgatggggca tttaaaatcc cttccgccaa tgagaatcct aaagcgaagt cctccaaatt
     7321 agccggtgtt gccatcatta cagtatgaga aataccgtta tataccaaag caaccggcac
     7381 ttctgaaatt aaattttctt catcatcttt ttgtttaata ctattagttg ataatgataa
     7441 ttttttggca tttatttttg tgatcttatt cacaatattt atctctcact tgactaaaaa
     7501 gtagtttatt ttgttaaaaa gttgtatcat taaattgttt aaatttggtt tacatcaaat
     7561 taatttaaga ttttatctat ttaccaaaaa aagagtatgt tcattttctt ttattagggg
     7621 gaaaatgata cacctcttag atcaatttat taaattaatt ttgatcaaat tttgttttat
     7681 ttctcacatt atgtgatttg gagtgattat gcagatctca agacgtaagt tcttcaaggt
     7741 ctgtgcaggt ggtatggcgg gaacttctgc tgcaatgttg ggttttgctc cagcaacagc
     7801 attagctgca ccgcgtgagt ataagctctt aagagcaaaa gaaactcgac aaacctgtac
     7861 ttactgcgcg gtaggctgtg gcatgattat gtacagctta ggcggcgaag caatgaacag
     7921 caaaggaaag ttaattcata ttgagggtga ttctgaccat ccagtaagcc gtggggcgtt
     7981 atgtccgaaa ggtgcagggg cgttggacta tgttaatagt gaccgccgtg tgcattatcc
     8041 ggaagtgcgt gaagccggtt caagtgagtg gaaacgcatt tcttggcacg aggcaattga
     8101 acgtattgcc cgccatatga aaaatgaccg tgatgagaac ttcgtcaccc acaatgaagc
     8161 agggctggaa gtaaaccgtt ggatgacggc aggtttcttg gcaggctcgg cgtgtagtaa
     8221 cgaaaccggt atcgtcaccc agaagtttat ccgctctctc ggtatgattt ttaccgacaa
     8281 ccaagcgagt atctgacacg gaccaacggt agcaagtctt gctccatcat ttggtcgcgg
     8341 tgcgatgacc aaccactggg ttgatattaa aaactccgat ttagtgatcg ttatgggcgg
     8401 taacgcagcc gaagctcacc ctgtgggttt ccgttgggca gttgaagcga aaaaacagaa
     8461 cggtgcgaag ttaatggtgg tagatccacg ctttaaccgt agtgctgcgg tggcggatat
     8521 ttatatacca ttgcgtccgg gtacggacat tgcgttcttg ttaggtgcgg taaactacct
     8581 gcttaaaaac gacaaaattc aacacgagta tgtgaagcat tacaccaatg ccacattctt
     8641 actcaacgaa gggtttaaat tcgaagacgg gcttttcacc ggctacgatg aagccactcg
     8701 tacctataaa gacaaatcca cttgggctta tcagttagat gaacagggtc agccgaaacg
     8761 ggatatgaca atgcaagatc cacgttgtgt gatcaatatg ctcaaagacc acgtttcccg
     8821 ctatacgcct gaaatggtag aacgcattac cggtacgcca caaaaagatt tcctgatttt
     8881 ctgtgaagag attgcgaaaa cctcagcgcc ggataaagcc gccaccttcc tctatgcctt
     8941 aggttggaca caacacaccg tgggcgcaca aaacattcgc tcaatggcga tcattcagtt
     9001 gctgtgcggt aacattggtg tctccggcgg tggggtaaac gcactgcgtg gtcactctaa
     9061 tgtacaaggg attaccgacc taggcttgtt cccacatatg ttgccgggct atatccgcct
     9121 gccaaccgaa gcggacgcaa ccttagaaag ctacttaaat gcgattgcac cgaaaacctt
     9181 agtggcaggg caagtaaact acgggcaaaa caacccgaaa ttcgctgtga gtatgctcaa
     9241 aaccttctac ggcgataagg caaccaaaga gaacgagttc ggctatcact atttaccgaa
     9301 atacccgaaa ggcggtacag atcacttccg ctttatcgaa cagatgtatc aaggcaaagt
     9361 gaacggctat ttctgccaag gtatgaaccc gattgcctct taccctaact cgcagaagtt
     9421 aattaaagcg ttgagcaatt tgaaatattt agtcattttt gacccgctta ttaccgatac
     9481 ttccgagttc tggaaaaatc acggtgagtt caacaatgtg aacccgtctg aaattcaaac
     9541 cgaagtgttc cgcttgccga ccacttgctt tgcggaagaa gatggttcaa ttgcgaactc
     9601 cggtcgctgg ttacaatggc actggaaagc agcagacgca ccacacgagg caaaaccgga
     9661 cgtggatatt ctgtctgaaa tccgtgccga actgatccac ctttatcata aagaaggcgg
     9721 aaaagcaccg cttgaaccgt tagaagcgat gacgtggaac tatatgaacc cgttagaacc
     9781 gaagccggaa gagatcgcca aagagaacaa cggttatgca ttggaagatc tgaaagatgc
     9841 agacggcaac atcatcgtga aaaaaggcga actattatcc agctttacac aaatgcgtga
     9901 tgacggtaca acctccggtg gttgttggat ctactgcggt caatggactc cgaaaggtaa
     9961 ccaaatggcg aaccgtgata actccgaccc atcaggctta ggtaacacct taggctgggc
    10021 attctcgtgg ccggtaaacc gccgtattct atacaaccgt gcatcggcag atttaagcgg
    10081 taaaccgtgg aacccgaaac gccagctagt taaatggaac ggtaaaaact ggaactatgt
    10141 ggacgttgcc gattacggta cagcaccacc aaatagccct gtgacaccat ttattatgca
    10201 accggaaggg gtatccggct tgttcgtgcg tgaacgtatg caagacggtc cattccctga
    10261 gcattatgaa ccgtttgaaa cgccaatcgg cacgaacccg ttacacccga atgtaatcag
    10321 cagtccggtg gcacgcattc tgccgagcga taaagaggat ttaggcacca gcgatgagtt
    10381 cccatatgtc ggtacaactt accgcttaac cgaacacttc cactactgga cgaaaaatgc
    10441 cctgattaac gtcattgctc aaccggagca attcgtggaa atcggcgaga aacttgccga
    10501 agagaaaggc attaaaaatg gcgacaaagt acgtgtttgg tctaaacgtg gcgaaattaa
    10561 agccgttgcc gtggtgacta aacgtatccg ttcattggtc tctgacggca aaccaatcca
    10621 cactatcggt gtgccgatcc actggggctt tgcggctaca accggtgcga aaaaaggcta
    10681 ttttgccaat aacttaaccg tgcgaacagg tgaaccaaac tcaaatacac ctgaatcgaa
    10741 atgtatgtta ttaaacattg agaaaattgt gggggcttaa caatgagtac agttcaaact
    10801 caaaacatta ttaaaatctc cgcaacctcg gcgctgacac cggcgccgca ggctcgttcg
    10861 caccaagtgg aagtagcgaa actgatcgat gtcaccactt gtatcggctg taaagcctgt
    10921 caagtgggct gttcagaatg gaatgacatt cgctccgatg taaatgcaaa ctgtgtcggc
    10981 gtgtacgaca accctgcgga tctaaatgcg aaagcgtgga cagtaatgcg ctttaacgaa
    11041 gtagaagaaa acgaccgttt agaatggttg atccgtaaag acggctgtat gcactgtgcc
    11101 gagccgggtt gcttgaaatc ttgcccgtca ccgggtgcga ttatccaata cgccaacggt
    11161 attgttgatt tccaatccga caaatgtatc ggttgtggct actgtatcgc aggttgtccg
    11221 ttcaacattc ctcgtatgaa tccggacgat aaccgtgttt acaaatgcac cctgtgtgtg
    11281 gatcgtgtca atgttggtca agaaccggct tgcgtgaaaa cctgcccgac cggtgcaatc
    11341 cgcttcggta gcaaagaaga gatgaagttc tatgcagaac aacgcattgc tgatctgaaa
    11401 tctcgtggct atgaaaatgc cggcttgtac gaccctgaag gcgttggcgg tacgcacgta
    11461 atgtatgtat tacatcatgc cgacaaaccg gagctttatt caggcttacc gaaagatccg
    11521 aagattgata ccacagtaac cttatggaaa gacgttttaa aacctgtggc ggcagtagcg
    11581 ttgggtggtt tagctttagc agaagttgca cactatatta ccgttgggcc aaacgttgag
    11641 gaagatcttg aagaacacca ccatagcgaa aatgcagaag gaggcaaaca tgagtaagaa
    11701 atttgaaatc acgaacgata ccaaaatcgt tcgtcataag cccttagctc gtgttagcca
    11761 ctggttctta gtaatctctt tttttatgac gatgtttacc ggtgttgcct tcttcttccc
    11821 tgactttgct tggcttaccg agattttagg tactccacag attgcccgtg cagtccaccc
    11881 atttaccggg attataatgt ttattgcatt tattattatg gcgttaatat attggcacca
    11941 taatatccct gagaaaaatg atattcgttg ggcaaaaggc attgttgaag tactaaaagg
    12001 taatgagcat aaggtttccg ataatggaaa atacaatttg ggtcaaaaat tactcttttg
    12061 gactcttatt cttgcgatgt tcactctttt agccacaggg attattatgt ggagacaatt
    12121 tttctctcac tacttctcga ttcctatgct aagaattgca attttacttc attcagcaag
    12181 tgcatttatg ctatttaccg gaattttagt acatatgtat atggcattct gggtaaaagg
    12241 ctcaatcaga ggtatggttg agggttgggt taccgttcgc tgggcaaaaa aacaccaccc
    12301 aagatggtac cgtgaagaag tgctacctga attagaaaga caagtagctg agcaaaataa
    12361 taaataacct caagtgcggt attattaccg cacttttatt ttattggttt aaaaagaata
    12421 ttatgagtat caaaatacta cctcaagatg atatcaaaca agcagctagt tcatttcagc
    12481 aacccgagct cttatttgct aatcccaaaa atctgtattt acgccgcgct aagcggctac
    12541 gtgaattagc caagcaaaac ccattcggtg aatatatgga gttttctgct aacctcgtgg
    12601 acattcaatt agcattactt gaaagccagc caattgcaga ttattctcaa aaaataaccg
    12661 cttgtgtaga gcgcacgcaa ggagaaacac cgctaaatgc acagaatttt cagcgtactg
    12721 atgaatggcg gacactactc ttagccatta ttgagaaatt caagccttat gcaaatgata
    12781 ctgttcttgc aacaattgaa gtattggaaa aggcagctaa gtcagagcta gacacacttg
    12841 ctgataattt gctaaatgaa cgatacgaac ttgtgggcgc agataaagct gtctttattt
    12901 gggctgctct ctcactttac tgggtacaat tagctcaaca attaccacgt aattctcgtg
    12961 cagaaatagg ccataaacat ttatgtccgg tatgtaactc agcacctgta agcagtgtga
    13021 ttcatttcgg tgaagctcaa gggttacgtt atctacactg ttctttatgt gaaagtgaat
    13081 ggcatgtagt acgtacaaag tgtacaaact gtgatgaaac cggcaaactg gattattgga
    13141 gcttagatac agtagaagca gcagttaaag ctgaaagttg tggcgactgc cattcctact
    13201 taaaagtact ctatcaagat aaagacgtga atgtggaacc tgttgctgat gatttagcaa
    13261 ctctattctt agattccgag atggaacaaa aaggtctttc aagaagtggg ataaatccat
    13321 ttttatttca agtagagtaa ctcatttaca acaaaaacgg agcataacgc tccgttttt
//
LOCUS       DANLZC010000063        13316 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.063, whole genome shotgun sequence.
ACCESSION   DANLZC010000063 DANLZC010000000
VERSION     DANLZC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13316
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.063"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            314..958
                     /gene="rppH"
                     /locus_tag="PXE44_001408"
     CDS             314..958
                     /gene="rppH"
                     /locus_tag="PXE44_001408"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDL5588920.1"
                     /translation="MIDFDGYRPNVGIVICNKHGQVLWAKRFGQNSWQFPQGGINEGE
                     NIEAAMYRELYEEVGLSKKDVRLLWASKYWLKYKLPKRLVRNEGSGPVCIGQKQRWFL
                     LQLIGDESLINLKTTKNPEFDGWRWVSFWYPVRQVVSFKRDVYRKAMKEFAQVLQNNI
                     QKATDDKTGEKPRIERGETSPHTYKKTSQDSAKAYKREARKPYRPWHQHNQKGK"
     gene            960..1748
                     /locus_tag="PXE44_001409"
     CDS             960..1748
                     /locus_tag="PXE44_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL5588921.1"
                     /translation="MTILLSCLFLGVFVGFLAGLFGIGGGLIIVPSLVFLLPMAGVPP
                     EYIMSTALGTSFSTIIITAFASAQRHHKLGNVDIQTSKYFIPALMVSVFLSGLVVSRL
                     DPQLMSRIFAVMVLYLAGKMIFSIKRAPKNKPLTVQTTIIGGSIIGALASMAGIAGGA
                     FIVPFLNDRGLEMKRAIGTSSFCGAFLGLSGTISFIVSGWGLEGMPDYSLGYVYLPAL
                     FGITATSFFTSKMGASAANALPVPMLKKAFAVLLVCIAINMFLK"
     gene            1756..2553
                     /gene="lgt"
                     /locus_tag="PXE44_001410"
     CDS             1756..2553
                     /gene="lgt"
                     /locus_tag="PXE44_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL5588922.1"
                     /translation="MNEQFIQFPQISPIVFEIGPIALRWYGLMYLAGFAFAYWLGMRR
                     AKNSNGVWTTEQVDSLVYTCFWGVVLGGRIGDVFFYSFDRFLQDPLYLFRIWEGGMSF
                     HGGLIGVIVAMIWVSYRQKRNFWQTADFIAPLVPFGLGMGRIGNFINDELWGRVTDVP
                     WAVLFPSGGYLPRHPSQLYEAFLEGVVLLFILNWFIQKPRPAGAVSGLFLIGYGTFRF
                     IVEYFREIDHNVNTTADLITRGQLLSLPMIIGGILIMAWAYKVNKVK"
     gene            2640..3308
                     /locus_tag="PXE44_001411"
     CDS             2640..3308
                     /locus_tag="PXE44_001411"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDL5588923.1"
                     /translation="MSKKYKVIGFDLDGTLINTLPDLTLVANSMFLEAGLPTVTQEKV
                     LTWIGKGADIFFQNAVAHTGRIFQADELVKMRVSFDKYYATYVCEESTLFPNVKETLE
                     ILKANDYPLVVITNKPTKLVEPVLSAFGIYHLFDETLGGQSLPKIKPHPDPMFFICEK
                     FGITPSELLFVGDSENDVIAAKAAGCDVVGLTYGYNYNVPIEQSNPTFVTSEFKDVLK
                     IVGL"
     gene            3378..3638
                     /locus_tag="PXE44_001412"
     CDS             3378..3638
                     /locus_tag="PXE44_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL5588924.1"
                     /translation="MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVI
                     NPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPAIGKEIL"
     gene            3635..3967
                     /locus_tag="PXE44_001413"
     CDS             3635..3967
                     /locus_tag="PXE44_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL5588925.1"
                     /translation="MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVP
                     ITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTVDIAQRHGKFIEALPESLLDEIM
                     ARVSVIFE"
     gene            4094..5125
                     /gene="trpS"
                     /locus_tag="PXE44_001414"
     CDS             4094..5125
                     /gene="trpS"
                     /locus_tag="PXE44_001414"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL5588926.1"
                     /translation="MSKPIVFSGVQPSGELTIGNYLGALRNWVKMQDDYDCVYCIVDQ
                     HAITVRQDPESLRKSTLDVLALYLACGIDPNKSTIFIQSHVPEHAQLAWVLNCYTYFG
                     EMGRMTQFKDKSARYEENVNVGLFTYPVLMAADILLYQANQVPVGDDQKQHLEITRDI
                     ANRFNALYGKKDSEGNLIEPVFAVPEVFIAKSGARVMSLLEPTKKMSKSDDNRNNVIG
                     LLEDPKAVAKKIKRAMTDGDEPPVVRYDVQNKAGVSNLLDILSAITGKTIAELETEFE
                     GKMYGHLKTEVADKVSELLTNLQERYHHYRQDEALLEKIYREGAEKARAQAKATLDKV
                     YELVGFVRY"
     gene            complement(5189..5944)
                     /locus_tag="PXE44_001415"
     CDS             complement(5189..5944)
                     /locus_tag="PXE44_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDL5588927.1"
                     /translation="MKFIKKLSYSVFLATVITACATTESINQEAAQSYAQVKTQARQQ
                     NAIDNSSATAKRIHTVFHKMKPYAERANQTGVPFHWEITVLKSDELNAWAMPGGKMAF
                     YTGLVERLNLSNDEIAVVMGHEMAHALKEHGKSDRTVSAITSIVGAIADVAVTASTGV
                     STEGLLSTGVDLIATKPFSRSQETEADEVGLILMAESGYNPSAAPNVWVKMSKANGDS
                     GLSIFSTHPSNADRQENLARLVPEAMKIYNARK"
     gene            complement(6000..6527)
                     /gene="ppa"
                     /locus_tag="PXE44_001416"
     CDS             complement(6000..6527)
                     /gene="ppa"
                     /locus_tag="PXE44_001416"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDL5588928.1"
                     /translation="MGLELVPAGKELPDDIYVVIEIPANSDPIKYEVDKETGALFVDR
                     FMATAMFYPANYGYVNNTLSSDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGG
                     DAKVVAVPHTKLSKEYDHIKDVGDLPALLKAQIQHFFESYKALEAGKWVKVEGWGDVN
                     EARQEILESFERAKK"
     gene            6744..6819
                     /locus_tag="PXE44_001417"
     tRNA            6744..6819
                     /locus_tag="PXE44_001417"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6777..6779,aa:Lys,seq:ttt)
     gene            6842..6917
                     /locus_tag="PXE44_001418"
     tRNA            6842..6917
                     /locus_tag="PXE44_001418"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6875..6877,aa:Lys,seq:ttt)
     gene            6939..7014
                     /locus_tag="PXE44_001419"
     tRNA            6939..7014
                     /locus_tag="PXE44_001419"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6972..6974,aa:Lys,seq:ttt)
     gene            7107..8141
                     /gene="mltG"
                     /locus_tag="PXE44_001420"
     CDS             7107..8141
                     /gene="mltG"
                     /locus_tag="PXE44_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL5588929.1"
                     /translation="MLKKILIALGLAGVIACGGLFYGYQKLTSLAEHPITVQPNQLFV
                     LEKGVSSQKLAALLEEQGIVTHDDADLIPYLMRLYPELSKFKAGAYSLAGLTTVKDLL
                     AHLSSGKEVQLNVQFIEGKTFKIWREQLAKADYLQHTLQGKSEKEIADLLGIPHEKIE
                     GWIAPDTYNYTPNSTDLALLKRAYQKQKTALEQAWKNRAENLPLATPYEMLILASIVE
                     KETGIATERPQVASVFINRLRNNMRLQTDPTVIYGMGDRYNGNIRRKDLEEATPYNTY
                     QIDGLPPTPIAMPSEASIKAVANPDNTPYLYFVADGTGGHKFSKTLNEHNKAVAEWIQ
                     IERKRKNEQK"
     gene            8152..8772
                     /locus_tag="PXE44_001421"
     CDS             8152..8772
                     /locus_tag="PXE44_001421"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL5588930.1"
                     /translation="MRGKFIVIEGLEGAGKTTAHQVILKELEQAGINDVVMTREPGGT
                     PLAEKLRHLIKHETEEAINDKAELLMLYAARVQLVENVIKPALAEGKWVLGDRHDMSS
                     QAYQGGGRKLDRHLLETLKESVLGTFEPDLTIYLDLDPVIGLERARGRGELDRIEQQS
                     LDFFYRTRERYLELTQHNDKAVIINAEQPIEQVTADIQQVIRNWLK"
     gene            8772..9749
                     /locus_tag="PXE44_001422"
     CDS             8772..9749
                     /locus_tag="PXE44_001422"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL5588931.1"
                     /translation="MYPWLESTYQQLTDSFLQGRGHHALLFKTDVGLGTDTLIYKVAE
                     WLLCSQKLRHQPCLECKSCLLMQSNNHPDFHILEPIEDKDIGIDQVRELIYKLQNFAQ
                     QGGNTVVYIRGVDRLTEASSNALLKTLEEPRENVYFLLEAPLQATVLATIQSRSQTWV
                     LYAPQASEVMAWLQTQFPAISTDDLEIALRLCHSRPLICKNFIENDRLQQRKTFLQTF
                     WRFYKSHDVFLLFQAFAQEKELILQQLEWLESFFSDALKAKMAISHGWLNPDLQNGIL
                     PFSQQLTTHGLLKGHQILQQTQRDLTEINAVNPELMLLDCLTKLVLVFE"
     gene            9880..10122
                     /locus_tag="PXE44_001423"
     CDS             9880..10122
                     /locus_tag="PXE44_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL5588932.1"
                     /translation="MARTTSVTLGEPLNDFVNEMVASGRYGSTSEVVRTALRMLEEKE
                     QYLAQLRQAIDDGDASGYATETIDEMMLRVKAELNV"
     gene            10115..10399
                     /locus_tag="PXE44_001424"
     CDS             10115..10399
                     /locus_tag="PXE44_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5588933.1"
                     /translation="MYKLSAQALRDQEKLFRYSISQFGVQQTESYFADLKHILNLLAD
                     FPTMGTSAEYIRGGLRMHTHQSHTIYYRIKADHIFIVRILHNQMNHKRYI"
     gene            10404..11192
                     /locus_tag="PXE44_001425"
     CDS             10404..11192
                     /locus_tag="PXE44_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchF/TatD family DNA exonuclease"
                     /protein_id="HDL5588934.1"
                     /translation="MKDLFIIDSHCHLDSLDYETRHKNVDEVIENAKARGVHHFISVC
                     TTVGRFEAMKNLTAHRNDVSLSCGVHPLNVEDEPFDYDKLFQFAQDPKVVAIGETGLD
                     YHYTPETKALQQSLFVQQIEIANKLNKPLIIHSRSARQDTMDMLEQNNAEKCGGVLHC
                     FTEDWAMAKRALDIGFYISISGIITFRNAEELREVVRKVPLDRLLVETDSPYLAPIPY
                     RGKPNQPAYVRETCEYVATLKGVSTEELARITTENVQHLFNIKL"
     gene            11202..11723
                     /locus_tag="PXE44_001426"
     CDS             11202..11723
                     /locus_tag="PXE44_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1523 family protein"
                     /protein_id="HDL5588935.1"
                     /translation="MRAILKYFLILVSLSFFIVLGGAVNYAMPSYEDTVVTGMEVRRM
                     DKDGIISKANPADGEVRDVYFLFTEDPETKKVMVYRNEDTGWGLPPYFKFGSADIQAK
                     AQAYANEKQRVQIKYYGWRINWLNEFRNIVSIKPLEEAETVSKPIMSYVLYAVLAFLF
                     FLSVQFIRGIFKD"
     gene            11798..12499
                     /gene="lpxH"
                     /locus_tag="PXE44_001427"
     CDS             11798..12499
                     /gene="lpxH"
                     /locus_tag="PXE44_001427"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDL5588936.1"
                     /translation="MTYYFIADLHLNENQPEITHLFLQFMQEKALLAEAVYILGDLFD
                     FWIGDDEQSELISTVKQAIKTLTTSGTKCYFICGNRDFLIGKRFSEQTGMVILPDYHV
                     IDLFGTSTLLCHGDTLCTDDVKYQQFRKKVHQKWRQAIFLMFPLSWRIRIAEKIRSKS
                     KQEKRDKSAEIMDVNAEVTAQIVAKYNTTQLIHGHTHRQAIHQHNGFTRIVLGDWKEN
                     YASILSVTEQGAEFI"
     gene            12496..13286
                     /locus_tag="PXE44_001428"
                     /pseudo
     CDS             12496..13286
                     /locus_tag="PXE44_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         3953 a         2835 c         2773 g         3755 t
ORIGIN      
        1 actctgcttt atttgcgaag caaaaattgc cctgcttatt cattttaaaa tttaaactta
       61 ttttgacgag aaaaaacgca acaacttgat ctttatccag atcattgctt aagaaaaaga
      121 tcgcattttt agatctgatt aattgctctg ttttacaacg ttccgcctgt ttatcctcaa
      181 cttataccac ttatattcaa tagttatcca caaagttatt cacaagcggt tatattttat
      241 ggtttttttg caaatcaacc caaagtatga aacaattctc ccattctaga atttttatca
      301 attaggtggt cgagtgatcg atttcgatgg ctaccgccca aatgttggaa tcgttatctg
      361 taataagcac gggcaagttc tgtgggcaaa acggtttggt caaaactctt ggcaatttcc
      421 gcaaggtgga attaatgagg gggaaaacat tgaagcagca atgtaccgtg agctttatga
      481 agaggtcggc ttaagtaaaa aagatgtacg attgctttgg gcgtctaaat attggctaaa
      541 atataaactg ccaaaacgct tggtacgtaa tgaaggctct ggtcctgtct gtatcgggca
      601 aaaacagcgt tggtttctac tgcaattaat cggtgatgaa agcctgatta atttgaaaac
      661 cacgaaaaat ccggagtttg acggctggcg ttgggtcagc ttttggtatc cagtccgcca
      721 agtggtctct tttaaacgag atgtttatcg caaagcgatg aaagagtttg cacaggtttt
      781 gcaaaacaat attcaaaaag cgactgacga taaaaccggt gagaaaccac gcattgagcg
      841 tggcgagact tcgccccata cttataaaaa aacctctcag gattctgcca aagcctataa
      901 acgagaagct cgtaagcctt atcgtccttg gcatcaacat aatcaaaaag gaaaataaga
      961 tgactatttt attaagctgc ctttttttag gggtgtttgt cggcttttta gccggcttgt
     1021 tcggcattgg tggtgggtta atcatcgtgc cgagtttggt cttcctactg cccatggcag
     1081 gtgtaccgcc ggaatatata atgtccaccg cactcggcac atcattttca accattatta
     1141 ttaccgcctt tgcatcggca caacgccatc ataagttagg caatgtcgat atccaaacca
     1201 gtaaatattt tattccagcg ttgatggttt cggtttttct atcgggcttg gtggtaagcc
     1261 gcctcgatcc gcaactgatg tctagaattt ttgcggtaat ggtactttac cttgcaggta
     1321 aaatgatttt ttcaataaaa agagcaccga aaaataaacc acttaccgtt caaacgacga
     1381 taatcggagg ttcaatcatc ggtgcgttag ccagtatggc agggatcgca ggtggagcct
     1441 ttattgtacc atttttaaac gatcgaggct tagaaatgaa acgtgccatc ggcacttcct
     1501 cattttgcgg agcattttta ggtttatctg gcacaatcag ctttattgta agtggctggg
     1561 gcttagaagg aatgcctgat tattctctcg gttatgttta tttaccggct ctgtttggta
     1621 ttacggcaac ttcatttttc acctcaaaaa tgggggcaag tgccgccaac gctctacctg
     1681 tgccgatgct gaaaaaagcc tttgcggtgc ttttggtctg tatcgccatc aatatgtttt
     1741 taaaataagg acactatgaa cgaacaattt atccaatttc cacaaattag cccgattgtg
     1801 tttgaaatcg gtccgattgc cctgcgttgg tatgggctaa tgtatttagc cggctttgcc
     1861 tttgcctact ggctgggaat gagacgggcg aaaaattcca atggagtttg gacaaccgag
     1921 caagtggata gcttagtcta tacttgcttc tggggcgtag tattaggtgg gcgtatcggc
     1981 gatgtgttct tttatagctt cgaccgtttc ttacaagatc cgctctacct ctttcgcatt
     2041 tgggaaggcg gaatgtcctt tcacggcggt ttaatcggcg tcattgtagc aatgatttgg
     2101 gtttcctacc gccaaaaacg caatttctgg cagacagcag attttatcgc accgctagta
     2161 ccgttcgggc taggtatggg gcgtatcggc aatttcatta acgatgaact ttgggggcgt
     2221 gtaaccgatg tgccttgggc ggtgttattt ccaagcggtg gctatcttcc ccgccacccg
     2281 tcacagcttt atgaagcctt tttagagggt gtggtgttac tctttatcct caactggttt
     2341 attcaaaagc ctcgcccggc aggtgcggtt tcagggttat ttttaatcgg ctacggcaca
     2401 ttccgcttta ttgtggaata cttccgagaa atcgaccata acgtcaatac caccgcagat
     2461 ttaatcactc gtgggcaact actctcttta ccgatgatta tcggcggcat actgattatg
     2521 gcgtgggcgt ataaagtaaa caaagtgaaa taaacgtagg ggcaagtcaa tatttgccct
     2581 cattacaaaa attgcaaaaa atttgcagaa aacgaccgct tgtgtttgaa ggatatacta
     2641 tgagtaaaaa atacaaagtc atcggtttcg acttagacgg aacgctaatc aacaccctcc
     2701 ccgatctcac tcttgtcgcc aattcaatgt ttctagaagc cggtttgcca acggttacac
     2761 aagaaaaagt gctgacttgg atcggtaaag gggcagatat tttcttccaa aatgcggttg
     2821 ctcacaccgg cagaattttc caagccgatg agttggtgaa aatgcgagtg agctttgata
     2881 aatactacgc cacctatgtg tgcgaggaaa gcaccctttt cccaaatgtg aaagaaacct
     2941 tagaaatcct caaagcaaac gactacccgc tggtggtgat taccaacaaa ccgaccaaat
     3001 tagtcgagcc ggtgctttcc gctttcggca tttatcattt attcgatgaa acccttggtg
     3061 ggcaatcgct ccccaaaatc aaaccgcacc ctgacccgat gttttttatc tgcgaaaaat
     3121 tcggcattac cccatcagaa ttactttttg tcggcgactc agaaaatgac gtgattgccg
     3181 ccaaagccgc cggttgtgat gtggttggac taacttacgg ctataactac aatgtgccga
     3241 ttgaacaatc taacccgact tttgtgacat ctgaatttaa agatgtgttg aaaattgtgg
     3301 ggttataaag aaaacatttg ttttcttttg atgtttgctt tagactttcc gaacctaaag
     3361 atttaaagga gtaaagtatg ccaacaatcg cattacgtca ggtcggtggc tcggttgcaa
     3421 tggtgattcc gcctgctttt ttagaggcac tcaagctctc cgtcggtagc aaagtggatt
     3481 tgtctttaaa cgacaaccaa ctcgtgatta atcctgcccg cccaaaagtg aaatacagct
     3541 tagaggaatt attaagacaa tccgattttg aggcagcaag gaatgaagaa agccaatcgt
     3601 ggttatccga gccggcaata ggaaaagaaa ttttatgaaa cgtggcgata tttactggct
     3661 cgatcttgat ccaaccttag gacacgaaca atcaggcttc cgccccgttg taattgtttc
     3721 cgcaactgca tttaatctag ccagtaaact gcctgtggtt gtgccgatta ctaacggtgg
     3781 agcttttgct gaaagactgg gctttgccgt ttcattaaaa gatgccggct taaaaacaac
     3841 agggattatt cgatgcgatc aacctcgcac agtcgatatt gcacaacgcc atggaaaatt
     3901 tatcgaagcc ttacccgaaa gcctacttga tgaaattatg gcaagggttt cagtgatttt
     3961 tgagtaagat acaagcggtc tgatttgctc tattttttgc aaaatcatca ataaatctga
     4021 ccgcttgtac ttttatccct tcccaataac cgttaagata acctatattt ttcaaatcaa
     4081 acgagaagat aaaatgagca aacctattgt atttagtggc gtgcagccct caggtgaatt
     4141 gactatcggt aactacttag gggcgttacg caactgggtg aaaatgcaag atgattatga
     4201 ctgtgtatat tgcattgtcg atcaacacgc cattaccgtt cgtcaagatc cggagtcgct
     4261 acgcaaatca actttagatg tgttagccct gtatttagct tgtggcattg atccaaacaa
     4321 aagcactatt tttattcaat cccacgtgcc ggaacacgcc caattagctt gggtgctgaa
     4381 ctgttacacc tatttcggtg aaatggggcg tatgacgcaa tttaaagata aatcggcacg
     4441 ctatgaagag aatgtcaatg tgggcttatt tacttatccg gtattaatgg cggcagatat
     4501 tctgctttac caagccaacc aagtgccggt aggcgatgac caaaaacaac atttggaaat
     4561 cacccgtgac attgccaacc gtttcaatgc cttatacggc aaaaaagatt ccgaaggcaa
     4621 cctgatcgag cctgtttttg cagtgccgga agtctttatt gcgaaatccg gggcaagagt
     4681 gatgtcgcta cttgagccga ccaaaaaaat gtcaaaatcg gacgataatc gtaacaatgt
     4741 gattggacta ttggaagatc cgaaagcggt agcgaagaaa atcaaacggg caatgaccga
     4801 tggcgatgaa ccgccagtag tgcgttatga cgtacagaat aaagccggtg tctcgaattt
     4861 attggatatt ctctccgcca ttaccggcaa aaccattgcc gaattagaaa ccgaatttga
     4921 aggcaaaatg tatggtcatt tgaaaacaga agtagcagat aaggtttccg aactattaac
     4981 caatctgcaa gaacgttatc accactaccg ccaagatgaa gcattattgg aaaaaatcta
     5041 ccgtgaaggg gcagaaaaag ctcgagctca agccaaagca acgttagata aggtatatga
     5101 attagtcggt tttgtgcgtt actaacactt aatgaaaaat gctcttgagg attcaagagc
     5161 atttttgcaa aaaagaaccg attgtcaatt atttacgggc gttataaatc ttcattgctt
     5221 ccggcacaag gcgggctaag ttttcttgac ggtcagcatt tgatgggtga gttgagaaaa
     5281 tggacaagcc tgaatcgcca ttggctttac tcatttttac ccaaacattc ggcgctgctg
     5341 atgggttgta gcctgattct gccattaaaa ttaacccaac ttcgtccgcc tcagtctctt
     5401 ggctgcgaga gaatggcttg gtggcaatca aatccacgcc agtgctgagt aaaccttcgg
     5461 tgcttacgcc tgttgaggca gtcacagcca catctgcaat agcacctaca atactggtaa
     5521 tagcgctaac cgtacggtct gatttaccgt gttctttcag tgcgtgagcc atctcgtgtc
     5581 ccattaccac tgcaatttca tcgttcgata aattaagtct ttccactaac cccgtgtaaa
     5641 acgccatttt accgcccggc atcgcccacg cattgagttc atccgatttc aatacggtaa
     5701 tttcccaatg aaaaggtaca ccagtctggt tggctctttc cgcataaggc ttcattttat
     5761 gaaaaacagt gtgaatccgc ttagcggtgg cagacgaatt gtcaatcgca ttttgctgac
     5821 gagcttgggt tttcacttga gcataacttt gagccgcttc ttggttaatg ctttctgttg
     5881 tggcacaagc ggtaatcact gttgctaaaa aaactgaata acttaatttt ttgatgaatt
     5941 tcattttata tcccacaaaa aatgccaaac aaatttgctt ggcattcaaa aataaataat
     6001 tatttcttag cacgctcgaa tgattctaag atttcttggc gagcttcgtt tacatcgccc
     6061 caaccttcta ctttcaccca tttgcccgct tctaatgctt tatagctttc gaagaagtgc
     6121 tggatttgtg cttttaataa cgccggtaag tcacctacat ctttgatgtg gtcatactct
     6181 ttacttaatt tggtgtgtgg aacagcaacc acttttgcat caccgccggc ttcatcggtc
     6241 atttttaaca caccgacagg acggcaacga atcaccgaac ccggttgtaa cggatatggt
     6301 gttggcacta atacatcaac cgggtcaccg tctgatgata aggtgttatt cacataacca
     6361 tagtttgccg gatagaacat tgcggttgcc ataaagcgat ccacaaataa tgccccggtt
     6421 tctttgtcca cttcatattt gattggatca gagtttgccg gaatttcaat tacaacataa
     6481 atatcgtccg gtaattcttt accggcagga actaattcta agcccatttt ggtttccttc
     6541 tataataggt gaaataaacg gcgtgattat agcctatttt cagaaaaaga taaaagcccg
     6601 tttctctttt cattttccgc caacaaatcc ggctaatttt ttgctaaatt gcttacaaac
     6661 cgcacactca accatttttc gataaaaaaa acttgccaaa gttttaaaaa tccgtaagat
     6721 atgcactcgt tacgcaagat tatgggtcat tagctcagcc ggtagagcag cggactttta
     6781 atccgttggt cgaaggttcg aatccttcat gacccaccat taatcttcaa aacacctcta
     6841 agggtcatta gctcagtcgg tagagcagcg gacttttaat ccgttggtcg aaggttcgaa
     6901 tccttcatga cccaccatta atttcaaaac tcctctgagg gtcattagct cagtcggtag
     6961 agcagcggac ttttaatccg ttggtcgaag gttcgaatcc ttcatgaccc accatttact
     7021 caaatcccat ttgcaaattt ttctgaaaaa acaaccgctt tatagtagaa ttcagtcaat
     7081 ttttatcact tttattttat tcatcaatgc taaaaaaaat ccttattgca ttaggcttag
     7141 ccggcgttat tgcttgcggt ggactgtttt acggctacca aaaactcacc tctttagccg
     7201 aacacccgat cacggttcag ccgaatcaac ttttcgtatt ggaaaaaggc gtttccagcc
     7261 aaaaattagc ggctctgtta gaagagcaag gcattgtcac ccacgatgac gccgatctta
     7321 tcccttattt aatgcgatta taccctgagt taagcaaatt caaggctggt gcttattcac
     7381 tcgcaggctt aacgacagta aaagatttgc tcgctcattt aagctccggc aaagaagtgc
     7441 aacttaacgt gcaatttatt gagggcaaaa cttttaaaat ttggcgggaa caactagcga
     7501 aagccgatta tttacagcac acgctacaag gcaaatcgga aaaagaaatc gctgacttac
     7561 tcggcattcc acacgaaaaa atcgaaggtt ggatagcccc ggatacttac aactacacgc
     7621 ccaattccac cgatttagcc ttgcttaaac gggcttatca aaagcagaaa acggcgttgg
     7681 aacaggcttg gaaaaataga gcggaaaatc taccgcttgc aacaccatac gaaatgctga
     7741 ttcttgcctc gattgtggaa aaagagaccg gcattgcgac cgaacgccca caagtggcat
     7801 cggtctttat taaccgttta cgcaacaata tgcgactgca aaccgatcca accgtcattt
     7861 acggaatggg cgatcgttat aacggcaata tccgccgcaa agatttagaa gaggcaacgc
     7921 catacaatac ttaccagatt gacggcttac cgccaacgcc gattgccatg ccgagtgaag
     7981 cttcgattaa agcggttgcc aacccggata atacacccta cctctatttt gtggcagacg
     8041 gcacgggcgg tcataaattc agcaaaacac tgaatgagca taataaagcg gtggcagaat
     8101 ggattcaaat tgaacgcaaa cgcaaaaatg aacaaaaata gtgagaacaa tatgcgtgga
     8161 aaatttattg taattgaagg cttagagggc gcaggcaaaa cgactgctca ccaagtgatt
     8221 ctcaaagagt tggaacaagc cggcattaat gacgtagtaa tgacccgaga acccggcggc
     8281 accccccttg ccgaaaagtt acgccattta attaaacacg aaaccgaaga agccattaac
     8341 gataaggctg agctgctaat gctctacgct gctcgtgttc aattagtcga aaatgtgatc
     8401 aaacctgccc ttgccgaagg caaatgggtt ctgggcgacc gccacgatat gtcttcccaa
     8461 gcctatcaag gcggtggacg taagttggat cgtcacttac tcgaaacctt aaaagaatcg
     8521 gtactcggca cattcgagcc agatttaacg atttatctcg atctcgatcc ggtgattggt
     8581 ttagaaagag ccagaggcag aggcgaatta gaccgcatcg aacagcaaag ccttgacttc
     8641 ttctaccgca cacgagagcg ttatttagaa ttaacccaac acaacgataa agccgtgatc
     8701 atcaatgccg aacaacccat cgaacaggtg actgcggata ttcaacaagt aattagaaac
     8761 tggctgaaat aatgtaccct tggctcgaaa gcacttatca acaacttacc gattcatttt
     8821 tacagggtcg aggtcatcac gccttactgt tcaaaacaga tgttggtctt ggtacagaca
     8881 ccttaatcta caaggtggct gaatggttgc tttgcagcca aaaactccgc catcaacctt
     8941 gtcttgaatg caaaagttgc ctgcttatgc aaagcaacaa ccaccccgat ttccacattc
     9001 ttgagccgat tgaagacaaa gatattggta tcgaccaagt gcgtgagttg atttataaac
     9061 tccagaattt cgcccaacaa gggggaaata cggtcgttta tattcgagga gttgatcgtt
     9121 taaccgaggc aagcagtaat gccctgctca aaaccttgga agagccacgt gaaaacgtct
     9181 atttcttgct cgaagcaccg cttcaagcaa cagttttagc taccattcaa agccgcagcc
     9241 aaacttgggt actttacgcc ccacaagcct cagaagtaat ggcttggcta caaacacaat
     9301 ttccggcaat ctcaaccgac gacttagaga ttgcactccg cctttgccac tcacgcccac
     9361 taatttgcaa aaattttatc gaaaatgacc gcttgcaaca gcgaaaaacc tttttgcaaa
     9421 ccttctggcg tttctacaaa agccacgatg tcttcttgtt attccaagcc ttcgcccaag
     9481 aaaaagagct gattttacag caactcgaat ggctggaaag tttctttagc gatgccctaa
     9541 aagctaaaat ggcaatcagc cacggttggc taaacccgga tctccaaaac ggcattctcc
     9601 cttttagcca acaactaacg acccacggct tacttaaagg gcatcaaatt ctccaacaaa
     9661 cccagcggga tttaaccgaa attaacgccg tcaatccgga attgatgttg ctagattgcc
     9721 tgacgaagtt ggttttggtg tttgagtaaa agccctctct catagaaggt agctttcaat
     9781 tagccgtaaa aagaggcttc tagccctaga atatagttta taacttgcaa aattatctat
     9841 ctttgttatt gtatgaataa ttcacacaag gagaatatta tggcaagaac aaccagcgtg
     9901 actttaggcg aacctttaaa tgattttgta aatgaaatgg tggcaagtgg gcgttatggc
     9961 tctaccagtg aagtagtgag aaccgcttta cgaatgcttg aggaaaaaga gcaatactta
    10021 gctcagttgc gacaagccat tgatgatggt gatgctagtg gttatgcaac cgaaacgatt
    10081 gatgaaatga tgttgagagt aaaggcggaa cttaatgtat aaactttctg ctcaagcact
    10141 ccgagatcaa gagaaacttt ttcgttacag tatctctcaa tttggtgttc aacaaacaga
    10201 atcttatttt gcagatctaa agcatatttt gaatttatta gctgattttc caacaatggg
    10261 aacttctgcg gaatatattc gtggtggctt gcgaatgcac acacatcaat ctcatacaat
    10321 ttattatcga ataaaagcag atcatatatt tattgttcgc atcttacata accaaatgaa
    10381 tcataaacgc tatatttaga aaaatgaaag acttatttat catagactca cattgtcatc
    10441 tagattctct agactacgaa actcgtcaca aaaacgtgga cgaagtaatt gaaaatgcca
    10501 aagcccgagg ggtgcatcat tttatctcgg tttgcactac agttgggcgt tttgaagcaa
    10561 tgaaaaactt aaccgcccat cgtaatgacg tttcactttc ttgtggcgtt cacccgttga
    10621 atgtggaaga tgaacctttt gattacgata aattatttca gttcgctcaa gatccgaaag
    10681 tggttgccat tggggaaact ggtttggatt atcactacac accggaaacc aaagcgttgc
    10741 aacaatcgct tttcgttcaa caaattgaaa ttgcaaacaa actcaataag ccactcatta
    10801 tccattctcg ttcggcacgc caagatacga tggatatgtt ggaacaaaat aatgccgaaa
    10861 aatgcggtgg ggtgttgcat tgctttaccg aagattgggc aatggcaaaa cgggctttag
    10921 acatcggctt ttatatctca atttcgggca ttattacttt ccgcaatgca gaagaactcc
    10981 gagaagtcgt gcgaaaagtg ccgttagatc gcttattggt tgaaacagat tccccttatc
    11041 ttgccccgat tccttatcgt ggtaagccga accagccggc gtatgtgcgg gaaacttgcg
    11101 aatatgtggc gacattaaaa ggcgtttcaa ctgaagaatt agctcgcatt acaaccgaaa
    11161 atgtgcaaca cttattcaac attaaattat aaggaactaa tatgagagca atacttaagt
    11221 attttctgat tcttgtctca ctatcatttt ttattgtatt aggcggtgca gttaattatg
    11281 caatgccaag ttatgaagat accgtagtaa caggtatgga agtacgccga atggataaag
    11341 atggtattat cagcaaagcg aatcctgctg atggcgaggt gcgcgatgtt tatttcttat
    11401 tcacggaaga tcctgaaacc aaaaaggtaa tggtttatcg taatgaagat accggctggg
    11461 ggttgcctcc ttatttcaaa ttcggatctg ccgacattca agccaaagct caagcctacg
    11521 ctaacgaaaa acaacgtgtg caaattaaat attacggctg gcgaatcaat tggctaaatg
    11581 aatttagaaa tattgtttca atcaagccat tagaagaagc agaaacagtc agcaaaccaa
    11641 ttatgagcta tgtactttat gccgtattgg cgttcttgtt cttcctctca gtacaattta
    11701 ttcgtggcat ttttaaagat taatgccgat taacctttga caaacggttc gattttgtaa
    11761 aaaattgaac cgttttcttt tttatcaaca atatcaaatg acgtactatt ttattgccga
    11821 tctccactta aatgaaaatc agcctgaaat tacccacctc tttttgcaat ttatgcaaga
    11881 aaaagcacta cttgcagaag cggtttatat cttaggtgat ctgtttgatt tctggattgg
    11941 cgatgatgag cagtctgaac tcatttcaac cgttaaacag gcaatcaaaa ccctcactac
    12001 aagcggtaca aaatgctatt ttatttgcgg aaatcgtgat tttttaattg gcaaacgatt
    12061 cagcgaacaa acgggtatgg ttattttgcc tgattatcac gttatagatt tattcggcac
    12121 atctaccttg ctttgccacg gcgatacgct ttgtactgat gatgtgaaat atcaacaatt
    12181 tcgtaaaaaa gtccaccaaa aatggcgaca agctatcttt ttgatgtttc cgctttcttg
    12241 gcgaattcgt attgcggaga aaattcgatc caaaagcaaa caagaaaagc gtgataaatc
    12301 cgctgaaatt atggacgtaa acgctgaagt taccgctcaa attgtcgcaa aatacaatac
    12361 aactcaactt atacacgggc atacgcatcg acaagcaatc catcagcata atggatttac
    12421 ccgcattgta ttaggcgact ggaaagaaaa ttacgcctct attttaagcg taaccgagca
    12481 aggagctgaa tttatatgat taatgtgatt attccttgct ataacgccga aaacaccctt
    12541 gttcgtgcag tgcaaagtgt attaaatcag tctggattaa atctattgtg gcttattgat
    12601 gatgcttcaa cggataatac gcttgcctta gctaaacatt tacaagccca agtgcctgat
    12661 aaaattagag tggaacaaat gcctcaaaat ggcggtgttg ccaaagcacg caactgggga
    12721 gcattacaaa gtgaaacaga tttaattgcc tttttagatg ctgatgatgc ttatgaaaac
    12781 ggggcattgc aagttgctga aaagattttt gagtttaaac cggaaaccgg cttggttcgg
    12841 ttagcattaa aaccggtaac ttagatcaac gttacagctc tcaccctaat tttgagttag
    12901 cttggcaata tatgcaaatg actggtgcag gcaacactgt atttcggcgt agtttcttcc
    12961 tcgcttgtgg cggctttcca caacatcagc tcttccaaga attaggtgga gaagatggtg
    13021 ctttaggcat agccactaca caaattagct ctgtagctac tgcatttaat gatgtcggtg
    13081 ttttacatta ctgccgagag ggtatgcacg cagaaagatt attaaatgcg attttattca
    13141 acgaaaaaga tcccaatgtt actgaagaaa aagttaaaca agccaaccta attaccgaaa
    13201 atattgttaa taatattaga aatctacaag actgtcttaa tagtaaaggg attggtatta
    13261 agccttatac gcttgaatgg agctaaaaaa gcatttgcag attttcccta aaaaaa
//
LOCUS       DANLZC010000064        13249 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.064, whole genome shotgun sequence.
ACCESSION   DANLZC010000064 DANLZC010000000
VERSION     DANLZC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13249
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.064"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(54..1775)
                     /gene="recJ"
                     /locus_tag="PXE44_001429"
     CDS             complement(54..1775)
                     /gene="recJ"
                     /locus_tag="PXE44_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL5588937.1"
                     /translation="MEKIIKRRPLLTTEIVSTNPLLNRIYQSRGIKNTQELEHNLKNL
                     HRPQQLANIEQAVDLLLQAFREKSRIIIVGDFDADGATSTSLALIALRQLGFDKVDYL
                     IPDRFSQGYGLSVAVAEMVLAKGADLVLTVDNGISSTEGIALLKSHQIQVLVTDHHLP
                     PESLPNADALVNPNLAFCEFPSKSLAGVGVIFYVMMALRSRMREQHLFENKPEPNFAE
                     LLDLVALGTVADVVPLDQNNRILVHQGLQRIRSGHCRLGIKALAEVGKRDLSTLQAAD
                     LGFAIAPRLNAAGRLENMSLGVELLICENMELARRLALDLDSLNQTRKEFEQEMKAEA
                     LSICANLPSLAQSEQAHGIVLYQADWHQGVIGILASRIKDQFNRPVIAFAQESEESEY
                     LKGSARSINGVHMRDLLEHIDMRHPDLIEKFGGHAMAAGLTIHQSKLDLFKQIFDQSI
                     NAIIEPEQLQGIIYTDGELNALEFTLANAEMIRQGGPWGQHFPEPSFEGEFSILQQKL
                     LAGKHLKLMLQSEKGQLFDAIWFNVDVRAFPDLSIKKARLVYRLDINEFRGEKSLQLK
                     VEYLKYI"
     gene            complement(1860..2543)
                     /gene="dsbC"
                     /locus_tag="PXE44_001430"
     CDS             complement(1860..2543)
                     /gene="dsbC"
                     /locus_tag="PXE44_001430"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDL5588938.1"
                     /translation="MKKNLITLGLMCLSTITFASDAKLKSQLEKIGATDVKISDSPLT
                     GFKMATSNEGVVYISQDGRYVIQGKVFELKNGKAIDITNQGLLAELNSYTNEMIVYPA
                     KNEKHVITVFMDITCHYCHLLHTKIKEYNDLGITIRYLAFPRGGMNTQTAKQMEAIWT
                     SADKVKALNDAEDGKLPTQIKTPDIVKKHYQLGIKFGVTGTPNMVTSEGELIGGYVEP
                     KELAKMLSE"
     gene            complement(2553..4082)
                     /gene="mepM"
                     /locus_tag="PXE44_001431"
     CDS             complement(2553..4082)
                     /gene="mepM"
                     /locus_tag="PXE44_001431"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDL5588939.1"
                     /translation="MQHVILARDRKRHKKHFKIAVFLLAVFSLFVDIVLALKDQSSIN
                     NTSSQEDIIAIDKQEKYQSINTNLAENNTLHQEQHSSFINYVTDLLTQSALVIEHDDE
                     IVDDQTATSYEDELVGQDDEVEEGIETVENQVPGEKLPEEAEKALEDLLDVADQALRI
                     QNQFSYTVARGDKLKDVLEQSGLTASVAKNLIKQHSALAQLEPGQQFYWVLDNDGELE
                     YMNWLVSEKEERVYERKENGKFSFQRFEKKGEWKLDVVRGTIQNSFAVSLKAVGLSDR
                     QINQLAVGLQSQIATNKLKKGDRFAILIKREYIDGKVTDLGNVEGIHVISSKKSYYAI
                     QADNGRYYSRYGETLSGGFARHPLLFAARVTSSFNPKRRHPITGRISPHKGVDYGVPI
                     GTPIIAPSDGVVEHVAYQARGAGRYIRIKHGHITTVYMHLSKTLVKPGQSVKKGERIA
                     LSGNTGGSTGPHLHYEFHINGRPVNPITVKLPGSNSGMGNKERQAFLNKAKNVESRLK
                     M"
     gene            4307..5053
                     /gene="znuC"
                     /locus_tag="PXE44_001432"
     CDS             4307..5053
                     /gene="znuC"
                     /locus_tag="PXE44_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDL5588940.1"
                     /translation="MVSLENIEVSFNGQKALQNINLSIYPNSITTIVGPNGGGKSTLL
                     KILLKLLSPNKGKVIHQKGLKIGYVPQKLHLEPSMPITVYRFLALKPHTTKEMINQAL
                     DLFSITHLTENSMQKLSGGELQRVLLARAILDRPQLLVLDEPMQGVDIIGQTELYQLL
                     NKTRDLLNCAILMVSHDLNIVMANTDEVLCINKHICCAGSPEVITHDPSFIHFFGDQF
                     ARNVAFYSHKHNHHHNLHGDICLGECSCEH"
     gene            5218..8031
                     /gene="sucA"
                     /locus_tag="PXE44_001433"
     CDS             5218..8031
                     /gene="sucA"
                     /locus_tag="PXE44_001433"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDL5588941.1"
                     /translation="MQYTHFDDWLASSALGGANQSYIEEIYEQYLENPANVDESWRNI
                     FDSFPKTPQVEQAHTPIRDYFRKLAREQQPQSVTIIDPEAGAKQVRLLQWINAHRFRG
                     YLEAKLDPINYYRWKTSSVPELDYKYHGFTDEDLNETFTVGRYVYDKDTMKLSELDKA
                     LKETYLGPIGLEFMHVQDIEQRNWLQAKMESVLNKPLFTKAEKLNLLSELTAADGLER
                     YLGAKFPGAKRFSLEGSDAFIPMMKEIIRHASRQGMTDVVLGMAHRGRLNMLVNVFGK
                     RPAELFDEFAGKHADESRTGDVKYHQGFSSNFAVDDKNVHLTLAFNPSHLEIVSPVVI
                     GSVRARQERMRDIEHEKVLAVTVHGDSAVAGQGVVQETLNMANVRGYKVGGTIRIVIN
                     NQIGFTTSNPNDTRSTEFCTDIAKMIQAPIIHVNGDDPEAVAFAARMAVEYRTKFKRD
                     IFIDLISYRRHGHNEADEPLATQPMMYSIIKKHPTPRKVYADRLITEGVLTNEQEIEM
                     LNLYRDALDNGECVVPEWREMDMAAVDWLQYLNYDWTALYESKFPEERFLTIAKRVCE
                     YPASLKAHSRVEKIYTDRKEMYEGKKLFDWGMAETMAYATLLDEGTHVRLSGEDAGRG
                     TFFHRHAVIHNQNDGTGYVPLTQLHANQGRFQVWDSVLSEEAVLAFEYGFATTDPKTL
                     TIWEAQFGDFANGAQIVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARVERY
                     LQLCAQQNIQVCVPSTPAQVYHMLRRQAIRKMRRPLVAITPKSLLRHPLAVSSLDELI
                     NGSFQNVIGELDNIDPKQVKRVVMCSGKVYYDLIEKRRENNQTDVAIIRIEQLYPFPL
                     EEMQQILEPYAHVKDFVWCQEEPLNQGAWYCSKHNFEASLPEDAKLTYAGRPASASPA
                     VGYPSLHAKQQKQLVEDALTIAE"
     gene            8134..9363
                     /gene="odhB"
                     /locus_tag="PXE44_001434"
     CDS             8134..9363
                     /gene="odhB"
                     /locus_tag="PXE44_001434"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDL5588942.1"
                     /translation="MTIEILTPDLPESVADATVATWHKKVGDNVKRDEILVEIETDKV
                     VLEVPASSDGILAEITQEQGATVVSKQSLGKLVVAKAGDISSATIEQKTESTPSDRKH
                     AAIENSHANADDQGPAIRRLLAEHDLQAADIQGSGVGGRITREDIEKEIAKRVQINQV
                     KGSATSQNTVSTVAFNSRSEKRVPMTRLRKRIAERLLEAKNTTAMLTTFNELDMQPIM
                     NLRKTYGEKFEKQHGVRLGFMSFYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIA
                     VSTPRGLVTPVLRDCDKMSMADIEKKIKELAEKGRDGKLTVEDLTGGNFTITNGGVFG
                     SLMSTPIINPPQSAILGMHAIKDRPVAIDGQVVIRPMMYLALSYDHRLIDGKESVGFL
                     VAVKELLEDPTRLLLEI"
     gene            9656..10816
                     /gene="sucC"
                     /locus_tag="PXE44_001435"
     CDS             9656..10816
                     /gene="sucC"
                     /locus_tag="PXE44_001435"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDL5588943.1"
                     /translation="MNLHEYQAKQIFKQYGLPVSQGIACKTAEEAVEAIKQLQGEQWA
                     IKCQVHAGGRGKAGGVKLVRNEAEVREFADKWLGKRLVTFQTDANGQPVNTLYLEETC
                     NIDRELYLGAVVDRSAQKVIFMASSAGGMNIEEVAEKTPELIHKAVIDPLTGGQGYQG
                     RELAFKLGLKGDEVKQFADIFVKLANLFVEKDLALLEVNPLVITKEGQLLCLDAKMSI
                     DSNALYRHPELKELQDPSQDDEREAEAEKWNLNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLYGGKPANFLDVGGGATKERVAAAFKLILSDPNVKAVLVNIFGGIVRCDLIADGVIA
                     AVNEVGVNVPVIVRLEGTNAAQGREILANSGLSILAASSLKQAAELAVKSAN"
     gene            10825..11694
                     /gene="sucD"
                     /locus_tag="PXE44_001436"
     CDS             10825..11694
                     /gene="sucD"
                     /locus_tag="PXE44_001436"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDL5588944.1"
                     /translation="MSILIDKNTKVICQGFTGGQGTFHSEQALAYGTQLVGGVSPNKG
                     GTTHLGLPVFNTVREAVEATGATATVIYVPAPGCKDAILEAIDAGIQLIVCITEGIPT
                     LDMLQVKQRLNQTGVRMIGPNCPGVITPDECKIGIMPGNIHKKGNIGIVSRSGTLTYE
                     AVKQTTDEGFGQSTCVGIGGDPIPGSNFIDILKLFQDDPETEAIVMIGEIGGSAEEEA
                     AAFIKENVTKPVVSYIAGVTAPKGKRMGHAGAIISGGKGTADEKFKILEDAGVTTVRS
                     LAEIGSALRKILG"
     gene            complement(11765..13210)
                     /locus_tag="PXE44_001437"
     CDS             complement(11765..13210)
                     /locus_tag="PXE44_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDL5588945.1"
                     /translation="MDSIILFFESVLTWIVNTIDGPLWDVTIILLLGAGLFFTLTTGF
                     VQLRLLPQSLREMWGGRSTEGKSLTPFQAFTTGLASRVGVGNIGGVATAIALGGPGAV
                     FWMWITALMGMSSAFAESSLAQLYKTKDPNGMFRGGPAYYITRGLKLPSLAIAFAVTL
                     IFTFGFAFNAVQSNSIVEATKNAWDWNPVYVGMTLVVITGLIISGGVKRIGKVSAQVV
                     PMMALFYLIIAVIILGMNIEKVPSIISLIIESAFDFSAAAGGTFGALVSKTMLMGIKR
                     GLFSNEAGMGSAPNSAATSDAKHPASQGLIQMLGVFVDTIVVCTCTAVIILMSNNYGG
                     ETLRGISLTQSALQFHLGEFGLHFLAFVILLFCFTSIIGNYAYAETNIRYIKNSPWFV
                     WGFRAIVLFFVYFGAVRDGGIVWAFADTVMASMAVINLVAILILAPIVWIILKDYLRQ
                     VKSGKEPVFRIEEHQDLIHKGVDPLIWKSKE"
BASE COUNT         4061 a         2864 c         2548 g         3776 t
ORIGIN      
        1 gattctacaa tacaaatata attaattcta ttaaatttac ataaaataac atttcaaata
       61 tatttaagat actccacttt taattgcaaa gatttctctc ctcgaaattc attaatatcc
      121 aacctataaa ctaatcttgc ctttttgatc gataagtcag gaaacgccct tacatccaca
      181 ttaaaccaaa ttgcatcaaa tagttgccct ttctctgatt gcaacattaa ttttaaatgt
      241 ttacctgcga gtaatttttg ttggagaatg ctaaactccc cttcaaaact aggttctgga
      301 aaatgttgtc cccaaggccc accttgccta atcatctcgg cattggctaa agtaaactct
      361 agtgcattta actcaccatc ggtataaatg atgccttgta attgctcagg ctcgataatt
      421 gcattaatag attggtcaaa tatctgttta aataaatcta attttgattg atggatagtc
      481 aaaccagccg ccattgcgtg tccaccaaat ttttcaatca agtctggatg tcgcatatcg
      541 atatgttcca gtaaatctcg catatgtact ccgttgatcg agcgagcaga accttttaga
      601 tattcacttt cctcactttc ttgtgcaaaa gcaatgacag gacggttaaa ttgatcttta
      661 atgcgagaag ctaagatccc aattacacct tggtgccaat ctgcttgata taacactatt
      721 ccgtgtgcct gttcagactg cgcaagactt ggcaaattcg cacaaatcga cagtgcttct
      781 gctttcattt cttgttcaaa ctcttttcgg gtttgattaa gagaatcaag atcaagcgca
      841 agccggcgag ctaattccat attttcacaa attagtaact caacccctaa agacatattt
      901 tctaaccgac cagctgcatt caaacgtggt gcgatagcaa aacccaaatc tgctgcttgc
      961 aaagtagaaa gatctcgctt gcccacctcc gctaaagctt ttatccctaa acggcaatgc
     1021 cctgaacgga ttcgctgtaa cccttgatgc accaatatcc gattattttg atctaacggc
     1081 actacatcag ccactgtgcc taaagcgact aaatccagta attcagcaaa atttggctct
     1141 ggtttatttt caaataaatg ttgttctctc atacgagaac gcaatgccat cattacatag
     1201 aagatcacgc ctaccccggc taaagattta gacggaaatt cacaaaaagc taaattgggg
     1261 tttaccagtg catctgcatt gggtaggctc tctggcggta aatgatgatc ggtaaccaaa
     1321 acttgaatct gatggctttt tagcagagca atgccttcag tcgaagaaat tccattatct
     1381 accgttaaga ctaaatccgc cccttttgct aataccattt cagcaaccgc tacactcaat
     1441 ccgtacccct gagaaaaacg atccggaatt aaataatcta ccttatcgaa acccaattgg
     1501 cgtaacgcaa taagggcaag cgatgtactg gttgcaccat ctgcatcaaa atcacccaca
     1561 ataatgatcc ggcttttttc acgaaaggct tgtaatagca agtcaacggc ttgctcaata
     1621 ttggcaagtt gctgtggtct atgcaaattc tttaaattat gctctagttc ttgcgtattc
     1681 tttatgcctc gactttgata gattcgattt aataacgggt ttgtagagac tatttccgtt
     1741 gttaataatg ggcgcctttt aattattttt tccacaatac tcttctttaa tgaaacaaca
     1801 agcggtcttc ttttgcaaaa attttacaaa aaaagaccgc ttgtattcaa gttaatgaat
     1861 tactcactta acattttcgc taattcttta ggctctacat aaccaccaat caactcacct
     1921 tctgaagtta ccatatttgg ggtacctgtc acaccaaatt taatccctaa ttggtaatgt
     1981 tttttcacaa tatccggcgt ttttatttgg gttggtaatt taccatcttc agcatcattc
     2041 agtgctttta ctttgtctgc tgatgtccaa atagcttcca tttgttttgc tgtttgggta
     2101 ttcattccac cacgcgggaa tgccaaatag cgaatggtaa tacctaaatc attatattct
     2161 ttgattttag tatgtagtaa atggcaataa tggcaagtaa tatccataaa caccgtaatc
     2221 acatgtttct catttttcgc tggatatacg atcatttcat tagtgtatga attcaattct
     2281 gccaacaatc cttgattagt aatatcaatc gctttaccat tttttagctc aaatacttta
     2341 ccttgaatta cataacgacc gtcttggctg atatatacaa caccttcatt tgaagtcgcc
     2401 attttaaagc ctgttaatgg agaatctgaa attttaacat ctgtggctcc aattttttca
     2461 agctggcttt ttagttttgc atctgaagca aacgtgatgg ttgataaaca cattaatcct
     2521 aaagtgatta gatttttttt cattgagaat ccttacattt ttaatctact ttctacattc
     2581 ttcgctttat ttaagaatgc ttgacgttct ttattaccca tacctgaatt tgagccaggc
     2641 aattttaccg taatcgggtt gaccggtcga ccattaatat ggaattcata gtgtaaatga
     2701 ggcccggttg aaccgcctgt attacccgat aatgctatcc gctccccttt tttaacactc
     2761 tgccctggtt ttaccaacgt tttgctgagg tgcatataaa cagtcgtaat atgtccgtgt
     2821 tttatccgaa tataacgtcc tgctccacgg gcttgatatg ccacatgctc tacgacacca
     2881 tcacttggtg cgatgatcgg agtacctatt ggcacgccat agtcaacccc tttatgcgga
     2941 ctaattctcc ctgtaatagg gtgtctgcgt ttggggttga aagaagaagt tactcttgct
     3001 gcaaataata atggatgacg agcaaatcct ccacttaatg tttcaccata tcgactataa
     3061 tagcgtccat tatctgcttg aatagcataa taactttttt tactgctaat tacatggatt
     3121 ccttccacat ttcctaaatc cgttactttg ccgtcaatat attcccgctt aatcagaatt
     3181 gcaaacctat cccctttttt taatttattc gtcgcaattt gtgactgcaa tccaaccgct
     3241 agctggttaa tttggcggtc tgataagcca acggctttta agctaaccgc aaaactattc
     3301 tgaatagttc ctcttacaac atctaacttc cattcccctt ttttctcaaa tctttgaaaa
     3361 gaaaatttac cattttcttt tcgctcataa acccgttcct ctttttcgga aaccagccag
     3421 ttcatatatt ctaattcacc gtcattatct aatacccaat aaaattgttg acccggttct
     3481 aattgtgcta atgccgaatg ttgcttaatt aaatttttcg caacggatgc agttaagcct
     3541 gattgctcta atacatcttt aagtttatca cctcgagcta cggtataact gaattggttt
     3601 tgaattcgta gtgcttgatc agccacatca agtaaatctt ctaatgcctt ttcagcttct
     3661 tctgggagtt tttccccagg aacttgattt tcaaccgttt caatcccttc ttctacttca
     3721 tcgtcttgcc caactaattc atcttcataa gaagtcgctg tttgatcatc aacaatttcg
     3781 tcgtcatgtt cgattactaa tgccgactga gtcagtaaat cagtcacata attaataaaa
     3841 ctggaatgtt gctcctgatg gagagtatta ttttccgcca aattagtgtt gatactctga
     3901 tatttttcct gtttatcaat ggcaataata tcttcctgtg aagatgtatt attgattgag
     3961 gattgatctt ttagtgcgag aacaatatca acaaatagac taaataccgc taataagaaa
     4021 accgcaatct taaaatgttt tttatgtcgc ttacgatctc tcgctaaaat tacatgttgc
     4081 aaaatatatc ccgctatttt taaaatttac taaaatttac atatttttct gagtagacta
     4141 ttttaccaag aaaaaattca tttaatctat tgtcacaaca cgaaaaatca gctaatttta
     4201 actcaatacc cctaattttt ttatatcaat gaaaatccat catcttaaac cgaatctaaa
     4261 taccactaca tttgaactat tcaaacagaa tttatccgcg acatcactgg tttctcttga
     4321 gaatattgaa gtgtctttca atggacaaaa agcattacaa aatattaatt tatctattta
     4381 tcctaactct atcacgacga tagtcgggcc aaatggtgga ggaaaatcta ccctgcttaa
     4441 aattttgcta aaattactta gcccaaataa aggaaaggtt attcatcaaa aaggactcaa
     4501 aattggctat gtaccacaaa aattgcattt agaaccctcg atgcccatta ctgtttatcg
     4561 atttcttgcg ctaaaaccac atactaccaa agaaatgatt aaccaagcac tagatctgtt
     4621 ttctattacc catctcactg aaaatagtat gcaaaaatta tcgggtggtg aacttcaacg
     4681 tgtattgctg gctcgggcaa ttttagatcg tccgcaactt ttagtgttag atgaaccaat
     4741 gcaaggcgtt gatattatag gccaaaccga actctatcaa ttacttaata aaacaaggga
     4801 tctccttaac tgtgctattt taatggtctc acacgactta aatattgtga tggcgaatac
     4861 agatgaagtg ctttgcatca ataaacatat ttgttgtgcc ggaagccccg aagtcattac
     4921 acacgatccg agctttatcc atttttttgg cgaccaattt gcacgtaatg ttgcatttta
     4981 ttcacataaa cataaccatc accataattt acatggcgat atctgccttg gtgaatgtag
     5041 ttgtgagcac tgataggcgt cctttttacc aaatgtttaa aatttgtaaa aataatgcta
     5101 aaaataaccg cttgcagcac aagatttatc taaaaatcag acttacttca cattttattt
     5161 gcttaaattg gagtataata agggcgtgat ttcataccac aaacaggtca ggtctttatg
     5221 caatatacac attttgatga ttggcttgca tcatctgcat taggcggtgc aaaccaatct
     5281 tatatagaag aaatttatga acagtatcta gaaaatccag caaatgttga tgaaagctgg
     5341 agaaacatct ttgattcatt tcctaaaact ccccaagttg aacaagctca cacccctatt
     5401 cgtgactatt tccgtaaatt agcccgtgaa cagcaacctc aatccgtcac tattattgac
     5461 ccagaagcag gggcgaaaca agttcgctta ctgcaatgga ttaacgctca ccgtttccgt
     5521 ggctatttag aggcaaaact agacccaatt aactattacc gttggaaaac ctccagcgtg
     5581 cctgaattgg actacaaata tcacggcttc accgatgaag atctcaacga aacttttacg
     5641 gtcggtcgct atgtctatga taaagacaca atgaagctca gcgaactgga taaagcccta
     5701 aaagaaactt atttagggcc tattggttta gaatttatgc acgttcaaga tattgaacaa
     5761 cgcaactggc ttcaagccaa aatggaaagc gtgctaaaca aaccgctgtt caccaaagca
     5821 gaaaaactga atttattaag cgaattgacc gctgccgatg gtttagagcg ttacttaggg
     5881 gcaaaattcc cgggggcaaa acggttctca ttggaaggta gtgatgcctt tattccgatg
     5941 atgaaagaaa ttatccgcca cgcttcccgt caaggtatga ccgatgtggt tttaggtatg
     6001 gcacaccgtg gtcgtttgaa tatgttagtg aacgtatttg gtaaacgccc ggcagaattg
     6061 tttgatgaat ttgccggcaa acacgctgat gaaagccgca cgggcgatgt gaaataccac
     6121 caaggtttct cctcaaactt tgccgtagat gataaaaacg ttcaccttac ccttgctttc
     6181 aacccatcgc atttagagat tgttagcccg gtggtaatcg gctcagtgcg tgcaagacaa
     6241 gaacgtatgc gtgatatcga acacgaaaag gtgcttgcgg tgaccgttca cggtgactct
     6301 gcagtggcag ggcaaggtgt agtgcaagaa accttaaata tggcaaatgt tcgtggctat
     6361 aaagtaggag gcactatccg tattgtgatc aacaaccaaa tcggatttac cacctctaac
     6421 ccaaatgaca cccgctccac cgaattttgt accgacattg ccaaaatgat tcaggcaccg
     6481 attattcacg ttaatggtga tgatccggaa gctgttgcct ttgcagcgag aatggctgtg
     6541 gaatatcgta ctaaatttaa acgtgatatt tttattgatt taatctctta ccgtcgtcac
     6601 ggtcataatg aagccgatga gcctttagca actcagccaa tgatgtatag catcatcaaa
     6661 aaacacccga cacctcgcaa agtctatgcg gatcgtttaa ttaccgaagg tgttttaacc
     6721 aacgagcaag aaatcgagat gttaaacctg tatcgtgatg ccttagacaa cggcgaatgc
     6781 gttgtgccgg aatggcgtga aatggatatg gcagcggtcg attggctaca atatctcaat
     6841 tacgactgga ctgccctata cgaaagtaaa ttccctgaag aacgattctt aaccattgcc
     6901 aaacgggtgt gcgaatatcc ggcttcgctt aaagctcatt ctcgggttga aaagatctac
     6961 actgatcgca aagagatgta tgaaggcaag aaattattcg actggggtat ggctgaaaca
     7021 atggcatacg ctactttgtt agacgaaggc acacatgttc gcctttcagg agaagatgcc
     7081 ggacgtggca ctttcttcca tcgccatgcg gtaatccata accaaaatga tggaacaggc
     7141 tatgtgccac ttactcaact gcacgccaat caagggcgtt tccaagtatg ggattccgta
     7201 ctctctgaag aagcggtact cgcctttgaa tatggctttg ccactaccga cccgaaaaca
     7261 ctgaccattt gggaagctca atttggagat ttcgctaacg gagctcaaat tgttatcgac
     7321 caatttatca gctcaggcga acaaaaatgg ggcagaatgt gcggtttagt gatgttatta
     7381 ccacacggct atgaaggtca agggccggaa cactcctctg cccgagtgga acgctattta
     7441 cagctttgtg ctcagcaaaa tattcaagtt tgcgtgccat caacaccggc acaggtttac
     7501 catatgttac gccgtcaagc gatccgcaaa atgcgtcgcc cattggttgc aatcacacca
     7561 aaatcattgc tacgccaccc acttgcggtg tcatctcttg atgaattgat taacggttca
     7621 ttccaaaatg tgattggcga gctcgataat atcgatccta aacaagtcaa acgtgtggtg
     7681 atgtgttcgg gtaaagtgta ctacgactta attgaaaaac gccgtgaaaa taaccaaacg
     7741 gacgttgcca ttattcgtat cgaacagctc tacccgttcc cacttgaaga aatgcaacaa
     7801 attctcgagc cttacgctca cgttaaagac tttgtctggt gtcaagaaga gccactaaac
     7861 caaggtgcgt ggtattgcag caagcacaat tttgaggctt ctctgccgga agatgcaaaa
     7921 ttaacttacg caggcagacc ggcttctgct tcaccggcgg tgggttatcc atcgttacac
     7981 gcaaaacaac aaaaacaact tgttgaagat gctttaacga tcgctgaata ataaaatggg
     8041 cgtatatacg ccctacaagc ggttcacttt taaccaaatt ttacaattta cgctgtcgga
     8101 gttttaaaac tctctcgaaa aaaggaataa gttatgacca ttgaaatttt aacccccgat
     8161 ttacccgaat ctgttgccga tgcgaccgtt gccacttggc ataaaaaagt gggcgacaat
     8221 gtaaaacgtg atgaaatttt agtcgaaatt gaaacagaca aagtcgtttt agaagtaccg
     8281 gcttcaagcg atggtattct tgcagaaatt acccaagaac aaggtgctac tgttgtatcc
     8341 aagcaatctc tgggtaaatt agtagtagct aaagccggtg atattagctc tgcaactatt
     8401 gagcaaaaaa cagaatccac gccatcagac cgcaaacacg cagcaattga aaacagccac
     8461 gcgaacgctg atgatcaagg gcctgcgatt cgccgtttat tagccgaaca cgacttacaa
     8521 gccgctgata ttcaaggcag tggtgtaggc ggcagaatca cccgcgaaga tattgaaaaa
     8581 gagattgcca aacgagtgca aatcaaccaa gtaaaagggt ctgcaaccag ccaaaacacc
     8641 gttagcactg tagcatttaa cagccgtagc gaaaaacgtg tgccgatgac acgactccgc
     8701 aaacgtattg ccgaacgctt actagaggca aaaaacacca ctgcaatgct cactactttt
     8761 aacgagctgg atatgcaacc aattatgaat ttacgcaaaa cctatggcga aaaattcgaa
     8821 aaacaacacg gtgtgcgttt aggctttatg tcgttctaca ttaaagccgt ggttgaagca
     8881 ttaaaacgct accctgaaat caatgcctcc attgatggcg atgatgtggt ttatcataac
     8941 tatttcgaca tcagcattgc ggtttctacc ccacgaggct tagtcacacc tgtgttgcgt
     9001 gattgcgaca aaatgagtat ggcagatatt gagaagaaaa ttaaagaact tgccgaaaaa
     9061 gggcgtgacg gcaaattaac cgttgaagac ttaaccggcg gtaactttac catcaccaac
     9121 ggcggggtat tcggttcatt aatgtccacc ccaattatca acccgccaca aagtgcgatt
     9181 ttgggtatgc acgctattaa agaccgccct gtcgccattg acggacaagt agtgatccgc
     9241 ccgatgatgt acctagcctt aagctatgac caccgtttaa ttgacggtaa agaatctgtc
     9301 ggtttcttag tggcagttaa agagttatta gaagacccaa cccgtcttct tttggaaatt
     9361 taatcgaaaa tatgaccgct tgtactttta gctacaagcg gtctgttttt aataaaattt
     9421 gacaaaatcc gataaacgct ctatttgagt tcccttctga gccacctgag cgactttgaa
     9481 tttgtaggaa aaatttagtg tttgagctta caaggcaagc gagttataaa tttttccgtc
     9541 aagcaaattc aaactaagcg aagaaagtgg cgaagcaggg tgtgctttct tttggttact
     9601 tttctttgca caagcaaaga aaagtaacat aaaaaaaacc aaacgaggaa actctatgaa
     9661 tctacacgaa tatcaagcaa aacaaatatt caaacaatac gggcttcccg taagccaagg
     9721 gattgcttgc aaaacggcag aggaagccgt tgaggcaatt aaacaactac aaggtgaaca
     9781 atgggcgata aagtgccaag ttcacgctgg tgggcgtggc aaagccggcg gggtgaaact
     9841 ggttcgcaat gaagccgaag tgcgtgaatt tgccgataaa tggctcggca aacgcttagt
     9901 taccttccaa accgatgcta atgggcaacc ggttaatacc ttatatttag aagaaacctg
     9961 caatatcgac cgtgaattgt acttaggtgc agtggttgat cgctccgctc aaaaagtgat
    10021 ttttatggca tcaagtgccg gtggtatgaa tattgaagaa gtagcggaga aaacccctga
    10081 attaattcac aaagcggtaa ttgacccact caccggtggg cagggttatc aagggcgtga
    10141 attagcattt aaattaggct tgaaaggtga tgaagttaaa caatttgcgg atattttcgt
    10201 gaaactggct aacctatttg ttgaaaaaga tttagcttta ttagaggtaa atccacttgt
    10261 catcactaaa gaagggcaac tactctgctt agatgcaaaa atgtcgattg acagcaatgc
    10321 tctctaccgc cacccagaac ttaaagagtt gcaagaccca tcgcaagatg acgaacgtga
    10381 agctgaagcg gaaaaatgga atctcaacta cgttgcacta gatggcaata tcggctgtat
    10441 ggtaaacggt gcggggctgg caatggggac gatggatatc gtcaaacttt atggtggtaa
    10501 accggctaac ttcttagacg ttggcggtgg tgcaaccaaa gagcgtgtag cagcagcttt
    10561 taaattgatt ttatctgatc cgaatgtgaa agccgtgtta gtcaatattt ttggtggtat
    10621 cgtccgctgc gatttgattg ctgatggcgt cattgctgct gtaaacgaag tcggcgtgaa
    10681 tgtgcctgtt attgtgcgtt tagaaggcac taatgccgca caggggcgtg agattttagc
    10741 caatagcggg ctgagtattc tagcggcatc atcattaaaa caagcggctg aattagcggt
    10801 aaaatctgca aattaggagg aaaaatgtcg attttaattg ataaaaacac aaaagtaatt
    10861 tgccaaggtt tcaccggcgg acaaggtact ttccacagcg aacaagccct tgcttatggc
    10921 acacaattag tcggcggagt ttcgccgaat aaaggtggca caacccattt aggcttaccg
    10981 gttttcaaca ccgtacgcga agcggtagaa gcaaccggtg caactgcaac cgttatctac
    11041 gttccggctc ccggctgtaa agatgctatt ttagaagcca ttgatgccgg catccaacta
    11101 attgtctgta ttactgaagg cattccaacc ttggatatgc tacaagttaa acaacgtcta
    11161 aaccaaaccg gcgtgcgaat gatcggtccg aactgcccgg gtgtgattac ccctgatgag
    11221 tgcaaaatcg gcattatgcc gggcaatatc cacaaaaaag gcaatatcgg tattgtttct
    11281 cgttcgggta ctttaaccta tgaagcagtt aaacaaacaa ctgacgaagg ctttggtcaa
    11341 tctacttgtg tcggtatcgg tggtgatccg attccgggat ctaactttat tgacatttta
    11401 aaactgttcc aagacgatcc tgaaactgaa gcaattgtga tgattggtga aatcggcggc
    11461 tctgccgaag aagaagcggc agctttcatc aaagaaaatg tcaccaagcc tgtagtcagc
    11521 tatattgccg gtgtcaccgc accaaaaggc aaacgaatgg gacacgccgg tgcgattatc
    11581 agcggcggca aaggaaccgc cgatgaaaaa ttcaaaatcc ttgaagatgc cggtgtgaca
    11641 accgtcagaa gtttagccga aatcggttca gctcttagaa aaattttagg ctaaactaaa
    11701 agttcaagca gtcatattta cccatttttt tgtaaatatg accgcttgtt tttttgctat
    11761 taaattactc tttcgatttc caaattaatg ggtcaacacc tttatggata aggtcttgat
    11821 gttcttcaat tctaaacact ggctctttgc cacttttcac ttggcgaaga tagtctttta
    11881 aaataatcca gacaatcgga gcaagaatta agatagccac caagttaatt accgccattg
    11941 atgccatcac tgtatcggca aatgcccata caatccctcc gtctcttact gcaccgaagt
    12001 acacaaagaa taaaacgatg gcacggaatc cccacacaaa ccacggactg tttttaatgt
    12061 agcggatatt agtttctgca taagcatagt taccgataat ggatgtgaag caaaacagta
    12121 aaatcacaaa tgctaaaaag tgtaaaccaa attcacctaa atggaattgt aacgcacttt
    12181 gagtaagtga aatacctctt aaagtttcac caccatagtt atttgacata agaatgatca
    12241 ctgcagtaca agtacagact acaattgtat ctacaaatac gcccaacatt tgaattaagc
    12301 cttgacttgc cgggtgtttc gcatcagaag tcgctgcaga atttggtgca gatcccatac
    12361 cagcttcgtt tgagaataag ccacgtttaa tccccattag catagttttg gataccaatg
    12421 ctccaaacgt tccacctgca gcagcagaaa aatcaaaggc gctttcaata attaaagaga
    12481 ttatacttgg cactttttca atattcatac ctaaaataat tactgcaata atcaagtaga
    12541 ataatgccat cattggaaca acttgggcag aaactttacc aatacgcttg acccctccag
    12601 aaataattaa ccctgtaatc accactaatg tcatgcctac gtagactggg ttccaatccc
    12661 acgcattttt ggttgcttcc acaatagagt tagattgcac agcattaaaa gcaaaaccaa
    12721 aggtgaagat aagcgtcact gcaaaagcaa tagcaagaga aggcagtttt aaaccacgag
    12781 tgatgtaata ggctggccca ccacgaaaca ttccgtttgg atctttggtt ttatacaact
    12841 gagccagtga agactcagca aaggcggaac tcattcccat taaggcggta atccacatcc
    12901 agaataccgc acccggccca cccaaagcga ttgcagtagc taccccacca atattaccaa
    12961 ccccaacacg gctggctaat ccagtagtaa aggcttggaa cggagttaat gatttccctt
    13021 ccgtagaacg tcctccccac atttcacgta aactttgcgg aagaagacgc agctggacaa
    13081 aacctgttgt aagagtgaaa aataaccctg caccgagcaa taggataatc gttacatccc
    13141 aaagtggacc gtcgattgtg ttcacaatcc aagtcagtac actctcaaaa aatagaataa
    13201 ttgaatccat aaagacctct taattgaaaa taaaacttcc acattgtaa
//
LOCUS       DANLZC010000065        13188 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.065, whole genome shotgun sequence.
ACCESSION   DANLZC010000065 DANLZC010000000
VERSION     DANLZC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13188
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.065"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..274)
                     /locus_tag="PXE44_001438"
     CDS             complement(<1..274)
                     /locus_tag="PXE44_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5588946.1"
                     /translation="MRIIKRRYPKLNANEITTDMLIEWRNQIVGDKIKAVTWNNYVRH
                     LKSLYNFGLSQGVLNINQNPFNQLFIRESKRRKKTYSTEQLHKIDHI"
     gene            complement(373..2352)
                     /locus_tag="PXE44_001439"
     CDS             complement(373..2352)
                     /locus_tag="PXE44_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5588947.1"
                     /translation="MQSIFKKIFGKHTSQSAVKSDTVFSIEQDDDGWLYPQNATQLLN
                     TELRQKYLGLLWQQVSMNRQMFETFYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKARQSYILPPNVAPEEQSKQRDAWTAAAIYLALVHDIGKVVVDIEITLKD
                     GSRWFAWNGEPPEPYKFKYIKGRDYHLHLVIGSFFANQIIPKSAFDWLAQYPEVISSL
                     MYAMSGHNDKAGLLSEIVQKADQYSVTLALGGDVNKLVQRPVNSLSKQLVLALRYLLE
                     QKIKLNTPKGPADGWFTEEGLWLVSKTAADNIRTYLLGQGISVPKDNPKLFDEMQSLG
                     IVEESPSGGAIWSCRIQADSGWTPANVFTLLRIKPEVAWENINDRPNYFSGKVKIVEQ
                     IMEDSNMENHPPLVDESTKLVTLENVQPETIITSKEVEISQVEASSDNAPFSEIIIND
                     ENDLTSFVLDMFAPTESSEPSLADISIGKGQEIATMPSKQEVQDKPTTKKLNVTQSAV
                     ETPETLTLSELQSQNKENSITGDNFVNWLKSGIATNKFAINKPTAKLHIVEDHLFLVT
                     PVIFEMYLQESGLPYDKEMINSLQYDFQGLGIHLPKNTTINGKPDTINFWKCDVIGPR
                     KTSSLTGYLIKNTHLFFGDKTLLNNHCLTLQKEET"
     gene            complement(2780..3577)
                     /locus_tag="PXE44_001440"
     CDS             complement(2780..3577)
                     /locus_tag="PXE44_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL5588948.1"
                     /translation="MENFEKQFISLFQRIVPHYRRSEVFYDFITLSALDMYLVTYRDQ
                     AEPNLRERFAHAKARYSDSEFTQLAELLAVTVNALTQKRYDFLGSVFMNLNLGDGYRG
                     QYFTPSHIADLMAKVTLQDCDRIISQQGFVTLSEPTCGSGVMVIGCVNAMFDAKYNPQ
                     QQLWVHCQDVDFTAAMMCYIQLSLLHIPACIVVGDTLLNETKIQMYTLAHLMGNWSHK
                     LEQQIAPLVNSTTEKGCGNNIEKSVIVEDLSQISILDEIDDNELIFY"
     gene            complement(3650..4396)
                     /locus_tag="PXE44_001441"
     CDS             complement(3650..4396)
                     /locus_tag="PXE44_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588949.1"
                     /translation="MPNWCENKLQIFCDCIQAKQIIPKLFRKTESDKWCLDFELLVPM
                     PESICIESSSLGDRAMEFLKISPNRRLTRPLLKKYIYGSEIDRIYAKAKHHRWDVGHF
                     INWLIKRPEQQSILWLNLALGQQYLSNLAQHGYKDWYDWSNEQWGCKWNVCSDSCQVS
                     FNDNGSINCLFDTPWGPPDGWFQALCTAFPDIEFILSFFEPGMWFAGKYIANLDGSYC
                     NIYIDDRGIRSFAEEVFNEEFEDEFEEKLE"
     gene            complement(4536..5510)
                     /locus_tag="PXE44_001442"
     CDS             complement(4536..5510)
                     /locus_tag="PXE44_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL5588950.1"
                     /translation="MNTQTKFDLYQHITDRIVAALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEYGYKQSKWITAKKANELGGYIRKGEKATVVMNYRPDK
                     REKLDEEGNIVYDEEGNPEIEQHFFIKKHNLFNLDQCENLPEELYDPISEPKQPTSDP
                     NQYEIFADIRQIIKGMDLHVEMKPSNRAYYQPSADLVVMPEIKQFENAQAFHSVLLHE
                     MTHATGAAKRLDREGITSGKAKFGNKVYAFEELVAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVIKEDKRAIVRASGKAREACQYMLDALNISQLADKNEVFEEAA"
     gene            complement(5755..6162)
                     /locus_tag="PXE44_001443"
     CDS             complement(5755..6162)
                     /locus_tag="PXE44_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5588951.1"
                     /translation="MKHDLDLLKYIWDNRNLNNKAYQYLFHICIEPMRKGDWNRYTIW
                     LNNNGNLVNISAVVYEIVSSQQLKDDIKLWQDMQFHCRPVKVETTGFISFLLHQFFNG
                     ITHVLLEQGMITETEAAEKSYHFIAANSFQRLL"
     gene            complement(6278..7120)
                     /locus_tag="PXE44_001444"
     CDS             complement(6278..7120)
                     /locus_tag="PXE44_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588952.1"
                     /translation="MSGSVLNTYFKHALVKAGFPNCLNIEYSLSHCQGDGVAFYGRLN
                     TETLINLFNKLNPQKRTQKMFSNLLNHIESWEPHYGVDFEIIRNSFGYRYSHCNTMEL
                     SVRTADDLAFFYDRDARKEWYFPTSKVEKYKALWDGFIQDLGQYIKDTSCALESNGYQ
                     IIEASPYQTETIFKFNTENYLIELKRKSSEFYSESVDWLCGDVCDFEAMIEEILQGKL
                     CYADFVATVTDKESGIQLGSDEITGVSYSPTDHSLSGYRQELISNAIASARSNAARFA
                     YLHA"
     gene            complement(7198..7593)
                     /locus_tag="PXE44_001445"
     CDS             complement(7198..7593)
                     /locus_tag="PXE44_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588953.1"
                     /translation="MARKKIQYTETDLHLYSVLWNLRHAHDLFSVKLERETKFGKAFS
                     VFSRFDVCPSYGEVDITYLVVKAGIRKTLRGFAYVGGNGFSHSRHLMSTFMYLVREIL
                     VEHNWISETELYDQPISVYKFRAINFDKW"
     gene            complement(7692..7937)
                     /locus_tag="PXE44_001446"
     CDS             complement(7692..7937)
                     /locus_tag="PXE44_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5588954.1"
                     /translation="MLVHIRLSCIGDVLPTKLNEWTSEHQALQQEISHRALEAWQNRD
                     KDIKSVRFMQQMPSRQGLNFRFLSVSEQNGKLIVSRA"
     gene            complement(8009..8362)
                     /locus_tag="PXE44_001447"
     CDS             complement(8009..8362)
                     /locus_tag="PXE44_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588955.1"
                     /translation="MSQQTAGWDVIPFESSKTVKPSLFWKLGNKLGNIFVSAKHKAKS
                     KYVEVTKGINQRIAEEDEMRALELQMCLDEQAAEYEQEISRIKRKMFFWTLLAAIIAF
                     VCGMAAVFGTQLSLI"
     gene            8736..9179
                     /locus_tag="PXE44_001448"
     CDS             8736..9179
                     /locus_tag="PXE44_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HDL5588956.1"
                     /translation="MKVSRFLGYCSIVTGVFSPLAQAQPNIIVYTTKMYPIQNSQLAS
                     QIYYLDQVENFEESISRNLSKSPEIAEQEMRAFFNSADWKAQEQQLKDAYQGVISGWQ
                     NGIKKVPAVFFDNPQSSPSVIYGTTDVQKALDEWHKWVKQQGESK"
     gene            9176..10117
                     /locus_tag="PXE44_001449"
     CDS             9176..10117
                     /locus_tag="PXE44_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HDL5588957.1"
                     /translation="MKKTFLTILISASLAVPTTLYPQPARASLNTASIAASSASTSCL
                     EYKVVGTCFWLFCTYFGCKVRTSTKIKHYLPEMVVSSYNHDGQTPWTELQALNPGVKG
                     GEYRSPQRQYSQLTFKNADAFGHPQGAIGQLLGNTGYYCKTQTTPFMPYFLSGLDFFA
                     WRENMPEMFYPEALTPGMREVGRVGDSWGNIYPRSGWVTQAHDYKASATIVQRVADIV
                     SRTAQPHIYFPAAKTGGAGEWPPGPVEEGQARTHKWQILYPQMSNTCSVFPNGGQTDT
                     YSSQISNQGDYAWALWRPYSCCKRRGQTFLYSIDWSH"
     gene            10135..12147
                     /locus_tag="PXE44_001450"
     CDS             10135..12147
                     /locus_tag="PXE44_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5588958.1"
                     /translation="MKSHFNFKKSHLRITTALLSSVSGLSYADSITDKLEFSKSDSII
                     SDRVFYQIGGGMGYMAPPSRGNTKVVDLGIGWKANLTCGNFDIKTTIKNDLNGLKDGF
                     KDLMGNVIDSAKGAVASMPAMVLQRANPQLYDLLTNGMYQGKLDFNRLKTSCEEMSKV
                     LADHTLGGKWGQAADLESYKQITSSQPDAKQAQKDLEDKKGKDGRVWIGGQKKGGENQ
                     EAINVVGDVVKAGFNLLNKRNALDTSTISGSSCDGNLCQTWDNPQTAADFLTKIIGEQ
                     SISTCKEDCGTKTSSKAGVGLSPEIEEEHVRTIVKLENVLNMDVPKAELLADLSSTTV
                     PVTRGLIESLRDDPDVEVLSQRLSAEIATSKIMEKMLLARRAVLAGMREPNVAQNADA
                     QVELERVLNIIDREISQVKLEMDMQKMITGNTASVILQSKSEREALAGAHSLSQDSLD
                     KRVQGLENGNVALTDLEAGSNINLSRRNIALSIPNIPNVAALIPNLPNRGGSGVGGNF
                     PSLAQISGSTMDQATGLLRNFEGFISKAQWDVNAHRVGYGSDTITRADGTIVKVTKDT
                     VITKEDAERDLARRTAIFANNVRKELGDSNWNALPPNAQAVLVSYAYNYGSLAKTKSV
                     LDAARRSAQSGDMNALATAIRNRQVDNNGINARRRNQEADYILGKN"
     gene            12168..12596
                     /locus_tag="PXE44_001451"
     CDS             12168..12596
                     /locus_tag="PXE44_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588959.1"
                     /translation="MRKLLGITVLTLCTTSAFATDKNVVFSCTSTEGKPLTVKRVGND
                     YEYSYDKTIFKNPIKKAVTNDGSIIARGSGFTTYALELKNDGLKYVVGFVQPNGNSKE
                     FIEPGATISRDNDQPSIGSVDCDPYKKIYYKFDVHLMNTL"
     gene            12812..>13188
                     /locus_tag="PXE44_001452"
     CDS             12812..>13188
                     /locus_tag="PXE44_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588960.1"
                     /translation="MDITTQNIELRYLSEQFYLDVAPHLKEILLKETRPYCVLLVRVK
                     NLDFALPLRSNLPEREGVGIKTIPNPQEYGKFKGIDFSKAILLTDKSYLKTDTVQLKN
                     KSELLNIQGNERRIVREFKKYVS"
BASE COUNT         4106 a         2548 c         2516 g         4018 t
ORIGIN      
        1 gtatgtggtc tattttatgt aattgttcgg ttgaataagt tttctttctt ctttttgatt
       61 cacgaataaa taactgatta aatggatttt gatttatatt gagtacccct tgcgatagac
      121 caaagttata taacgatttt aaatgtctga cataattatt ccatgttaca gctttgattt
      181 tatcgccaac aatttgatta cgccattcaa ttagcatatc agtggtaatc tcatttgcat
      241 ttaactttgg gtaacgtctt ttgataattc ggattacatt attatagctc cgcttagtat
      301 cagggcgaag atagtaacga tagaaaaaaa aatcggtcta gtagcgaatc aaaagagata
      361 gatgtcgtca ttttaagttt cctctttctg taaagttaaa cagtgattat ttaatagtgt
      421 cttatcccca aagaagagat gtgtattttt tatcaaatat ccagttaaag aggaagtctt
      481 tctcggacca attacatcac atttccaaaa attgatggta tctggcttac cgtttattgt
      541 cgtattctta ggtaaatgga ttcctagccc ttggaaatca tactgaagac tatttatcat
      601 ttccttatca taaggcagac cactctcctg caaatacatt tcaaaaataa cgggggtaac
      661 taaaaacagg tggtcttcaa caatatggag cttggcagta ggcttgttaa ttgcaaattt
      721 gttggtcgca atacctgatt tgagccaatt tacaaagtta tctcctgtaa ttgagttctc
      781 cttattctgt gactgtagct ctgatagtgt taatgtttcg ggcgtttcta ctgcactttg
      841 tgttacgttg agctttttag tggtaggttt atcctgtact tcttgtttgg atggcatcgt
      901 tgcaatttcc tgtcccttac caattgaaat atcagcaagg cttggctcag agctttccgt
      961 aggtgcaaac atatccagca caaaagaggt taaatcattt tcatcattta tgatgatttc
     1021 tgagaacggt gcattatctg agcttgcctc tacctgagat atttcaactt ccttagaggt
     1081 aataatagtc tctggttgaa cattctctaa tgtaacaagt ttagtgcttt catcaactaa
     1141 tggaggatga ttttccatat tgctatcttc cattatctgc tcgactattt taactttccc
     1201 ggaaaaataa tttgggcgat cattaatatt ctcccaagcc acttcaggtt taattctcaa
     1261 taatgtaaag acgtttgccg gtgtccaacc tgaatcagct tgaatacggc aactccaaat
     1321 cgccccacca gaaggagatt cttctacgat accgagagat tgcatttcat caaataactt
     1381 tggattatcc ttaggcacgg aaatcccctg ccctagtaag taagttcgta tattatcagc
     1441 ggcagttttg ctaacaagcc acaatccttc ctcggtaaac caaccgtctg cagggccttt
     1501 cggagtattt aacttgattt tctgttccag taaataacgt agtgccaaga caagctgctt
     1561 tgacagagaa ttgacaggac gttgaaccag tttatttaca tcgccaccga gtgcgagggt
     1621 aacactatac tgatcagctt tttgcacgat ttcagataaa agcccagctt tatcattatg
     1681 tccggacatt gcatacatca gagaagaaat gacttctgga tactgtgcca gccaatcaaa
     1741 tgcagatttg ggaataatct gatttgcaaa gaagctgcct atcacaagat gaagatggta
     1801 gtctcgacct ttgatgtatt tgaatttata cggttcagga ggttctccat tccaagcaaa
     1861 ccaacgtgag ccatctttga gtgtgatttc aatatctact acaactttgc caatatcgtg
     1921 aactaatgca agataaattg ctgcggcagt ccaagcgtct cgctgctttg actgttcttc
     1981 gggagccaca ttcggtggca aaatataact ctggcgagct ttagctgaaa aagcaataac
     2041 ttccaaaccg tgatcgagca ttccaccaat atgagaatga tgatgtgatt ctgaagctgg
     2101 caatagttga accatttcag cgtaacgttc tattggttgc tgataaaatg tctcgaacat
     2161 ttgacggttc attgatacct gttgccaaag tagtccaaga tacttttgac gtaattccgt
     2221 attcagtaat tgagtagcat tttggggata tagccaacca tcgtcatctt gctcaataga
     2281 gaaaacagtg tcagatttga ccgcactttg agatgtgtgt ttaccaaaaa ttttcttgaa
     2341 aattgattgc attgaaagag accgaaaagt atcaattttg tgttaaatga aagccgttta
     2401 gccctttgcc cttttgggca agacctttcc ccttggaaag gttacctttc accctttagg
     2461 ggttatcctt tgcccttaag gactccttta tccttttgcc atttagggtg agcttggaat
     2521 atattttgta gtgtataaat atatttctgt taagttatct aaaaactctc aaatattttt
     2581 aaatagtttt tgtatcaatt tacactaatg ctaggagaat atagacttta aatgctattg
     2641 acacaattca cagagttaaa ttaaggtagt aaatgctgtc ggcaatatcg gttggtatca
     2701 atagtcaaat atttattgaa aattcagttg ctgatataaa ataatttagc caccttattt
     2761 ctaaggcggc tattatttgt tagtaaaata ttagctcatt atcatcaatt tcatctaaaa
     2821 ttgagatttg agataaatct tccactatca ctgatttttc tatattgtta ccacagcctt
     2881 tctcagtagt gctatttaca agtggtgcaa tttgttgctc cagtttatga gaccaatttc
     2941 ccatcaaatg agccagcgta tacatttgaa tttttgtttc attcaatagc gtatcaccaa
     3001 ctacaataca agcaggaata tgtaataacg atagctggat ataacacatc atggcagctg
     3061 taaaatcaac atcttgacaa tgaacccaaa gttgttgttg gggattgtat ttcgcatcaa
     3121 acatcgcatt aacacaacca atcaccatta cacccgaacc acaagttggt tctgataaag
     3181 taacaaatcc ctgttgtgag atgattctat cacagtcctg tagtgtaact ttcgccatta
     3241 gatctgcgat gtgagatggt gtaaagtatt gccctctata accatcacca agattgagat
     3301 tcataaatac tgagcctaag aaatcatagc gtttttgtgt taaagcattc accgtaacag
     3361 ctaacaattc tgctaattga gtaaactcag aatctgaata tcgtgcctta gcatgagcaa
     3421 agcgttctcg caaattaggt tctgcttgat ctcgatacgt tactaggtac atatcaagtg
     3481 cagagagtgt aataaaatca taaaacactt ctgaacgtcg ataatgtggt acgatacgtt
     3541 gaaataagct gataaattgc ttttcaaagt tttccattag actactcctt aaagtttacg
     3601 gagtaatccc cctagggaat tacccctagg gtggaaaaat taattaaggc tattcaagtt
     3661 tctcttcgaa ctcatcttca aattcttcat taaagacttc ctcagcaaaa ctgcgaatac
     3721 ccctatcatc tatgtaaata ttgcagtatg agccatcaag atttgcgata tatttgcccg
     3781 caaaccacat tcccggctca aaaaatgaaa gtataaactc aatgtcagga aaggcagtac
     3841 aaagtgcttg gaaccaacca tcaggtggtc cccatggcgt atcaaatagg caattaatag
     3901 atccattatc attaaagcta acttgacagc tatctgaaca tacattccat ttacagcccc
     3961 actgttcatt tgaccagtca taccaatctt tgtaaccatg ttgagctagg ttagacagat
     4021 attgctggcc taatgcgagg tttagccata atatactttg ttgttcagga cgttttatca
     4081 accagttgat aaaatgacct acatcccagc gatgatgctt tgccttagca tatatccgat
     4141 caatttcgga cccatagata tactttttga gtagagggcg tgttaaacgt ctatttggtg
     4201 aaatttttag aaattccatt gctctatcac caagactact tgattcaatg cagattgatt
     4261 ctggcattgg aacgagtaat tcaaagtcta aacaccattt atcactttca gtttttctga
     4321 aaagcttagg aataatttgt ttagcttgga tacaatcgca aaaaatttgt agtttatttt
     4381 cacaccagtt tggcataatg tactcctaaa taaagtaggg gtacacttgc cttgaggcaa
     4441 tgtaccccaa gggttaaaag ataatcgata cataattcct aaaggattat gtattactat
     4501 gaaagattgt gttgaaagta cctttcagca aagagttaag ctgcttcttc aaagacctcg
     4561 tttttatcag ccagctgtga gatatttaat gcatctaaca tatattgaca agcttcacga
     4621 gctttaccag aagcccggac aatagcccgt ttatcttctt ttataactct tatccaactt
     4681 tcaatatatg ccgcattttg aggtaccgta tcaaatccaa ggtaggcaca caaaaatgct
     4741 ccccccattt cggcaaccaa ctcctcaaaa gcatagactt tgtttccaaa ttttgctttt
     4801 ccggaggtaa taccctcacg atcgagcctt ttagcagcac cagttgcatg agtcatttcg
     4861 tgcaataaca cactatgaaa cgcttgagca ttttcaaact gttttatttc cggcattaca
     4921 actaaatctg cactgggttg ataatatgcc cgattactcg gtttcatttc cacgtgtaaa
     4981 tccattcctt tgataatctg ccgaatatca gcaaaaatct cgtattggtt tggatccgat
     5041 gtaggctgtt taggttctga tataggatcg tacagttcct cgggtaaatt ttcacactgg
     5101 tctagattga acagattgtg cttcttaatg aaaaagtgtt gttcaatttc tggattacct
     5161 tcctcgtcat acacaatatt tccctcctca tcaagttttt ctctcttatc aggacggtag
     5221 ttcattacaa cagtagcttt ttcaccttta cggatatatc cgccgagctc gttagctttt
     5281 tttgctgtaa tccacttact ctgtttgtaa ccgtattctg cggtagcaat ccagagtaat
     5341 agtgtgttga taccagaata caatcggtta gagaccgcat tacgaggtaa tgccatatta
     5401 cattctggat gactccacgg ttttaaccat ggtgcagtcc cctgttccag tgctgcaaca
     5461 attcggtctg tgatatgttg gtataaatca aatttagttt gagtgttcat tagtgtttct
     5521 ccaaagttca ggtagaaaat ggggaaacac tattaccctt atcgggcact agtttccccg
     5581 atgggtggtt taaaatctca acagagatag tttttctagg ttcatcaaaa ccagtagaac
     5641 tgcctataat gcagtaacta cagataattt gttaatcagt attgattcaa gttaaacata
     5701 atcctcatat cgaaaattac tttggtaata caaagttaca ttatttcaga tccattaaag
     5761 taaacgttga aagctattag cagcaataaa gtgataactt ttttccgcag cttctgtttc
     5821 cgtaatcatt ccctgctcca gcaaaacatg ggtgattcca ttgaaaaact gatgtaatag
     5881 aaatgaaata aaacctgttg tttcaacttt tactgggcga caatgaaact gcatatcctg
     5941 ccaaagttta atatcatctt ttaactgttg agagctcaca atttcatata caacagcaga
     6001 aatattaact aaattaccgt tgttattcag ccaaattgta taacgattcc aatctccttt
     6061 tctcattggc tctatacaaa tgtgaaatag gtattggtag gctttattat ttaaattgcg
     6121 gttatcccag atgtatttga gtaaatctaa atcgtgtttc ataatgtttc tccaaaagaa
     6181 aaaagtggag aaacatctta cctataggga aaatatttcc ccacagggta aaaaacaatc
     6241 tcgataagcg gtcaaattga aataaaaaac ctacaaatca ggcatgcaag taggcaaaac
     6301 gtgcggcatt actacgagca gaagctatag cattactaat aagctcttgt cgataaccac
     6361 tcaatgaatg gtctgtcggc gaatagctta cgcctgtaat ttcatcgcta cctagttgaa
     6421 tgccactttc cttatcagta acggttgcta caaaatcagc atagcaaagt ttcccttgta
     6481 ggatttcctc tatcattgcc tcaaaatcgc agacatcacc acatagccaa tcaacagact
     6541 ctgaataaaa ttcagaggat ttacgtttaa gttctatcag atagttttcc gtgttaaatt
     6601 tgaaaatagt ttccgtctga tacggtgagg cctcaataat ctgatagcca ttcgattcta
     6661 aggcacagga agtatcttta atatactgac ctaaatcttg aataaatcca tcccacaggg
     6721 ctttgtattt ttccactttt gatgtaggga aataccactc ttttcgggca tcacgatcat
     6781 agaaaaatgc caaatcatct gctgttctaa cagaaagctc catcgtatta caatgcgaat
     6841 agcggtaccc aaaactattt cgtatgattt caaaatccac gccatagtga ggctcccaag
     6901 attcaatgtg attcagcaga ttagaaaaca ttttttgtgt gcgtttctga ggatttagct
     6961 tgttgaaaag attaattaac gtctccgtat taagcctacc gtagaatgct acgccatcgc
     7021 cttgacaatg agaaagtgaa tactcaatgt ttaagcaatt aggaaaacca gctttaacaa
     7081 gagcgtgttt aaaataagtg tttaagactg agccagacat tgcgcttctc ctaaaattag
     7141 agggaaacac attacccttt tccggaaatt gtgtttcccg aaagggtgaa agattgatta
     7201 ccacttatcg aagttaatcg ctctgaattt ataaacggat ataggttgat catagagttc
     7261 tgtttccgaa atccaattgt gttcaaccaa gatttctctg acaagataca taaatgtact
     7321 catcaaatga cgactatgac taaacccgtt tccaccaaca taagcaaacc ctctcagggt
     7381 tttgcgaatt cctgctttaa caacaaggta agtaatatca acctcgccat aagacgggca
     7441 gacatcaaag cgagaaaaaa cagaaaatgc tttgccaaac ttggtctcac gctctaattt
     7501 cacactgaat agatcatgtg catggcgtaa attccaaagt acactgtata agtgcaaatc
     7561 tgtttcggta tattgaattt ttttacgagc cataatgttt ctcctaaaaa ataaaaggga
     7621 aacattactt ccctctgggg ataatgcctc cccttgtgag gtataagaat aggatgtgaa
     7681 tatgaagttg cctatgctcg actaacaatt agcttgccgt tctgctcgga cacactcaga
     7741 aagcgaaaat taagtccttg ccgagacggc atttgttgca taaatcgcac gcttttgatg
     7801 tccttatctc gattttgcca cgcttctaaa gctctatgac taatttcttg ctgtaaggct
     7861 tgatgctctg atgtccattc attaagttta gttggtaaaa catctccaat acaggaaagt
     7921 ctaatatgaa ctaacataca tttctccaga ataaggggaa atgcattccc cttaagggaa
     7981 acaacatttc cccgatgggt gagttaaatt agattaaaga gagctgagta ccaaaaacgg
     8041 cagccatacc acatacaaag gcaataatgg cagccaacaa agtccagaaa aacatcttac
     8101 gcttaatgcg ggaaatttcc tgttcatatt cggctgcttg ttcgtctaag cacatttgca
     8161 gttctaaggc tcgcatttca tcttcttcag caatccgttg attgatgcct ttggtgactt
     8221 caacatactt gcttttggct ttatgtttcg ctgaaacaaa aatattacct agcttgttgc
     8281 caagtttcca aaaaagagaa ggttttacgg ttttggatga ttcaaacggg ataacatccc
     8341 atcctgcggt ttgttgactc atagcgagtt ctcctaaaag ttaagtgaat aaaattcctc
     8401 aggagatcgc aattccaagg gaattgaaat atccctcgga aggtaggtgt gtaggtagca
     8461 ttcctgcaaa tttgcagagt tccctttcag aatgctggga gacattttgg gtaaataact
     8521 caactacctc taaggatagc cgccgctttt caagagttat gcgactttta agggctgtat
     8581 ctacagcgtc ttaatgctta atatcatacg aattcctcca ataaaatcaa ccgttttttt
     8641 gttcaaaaaa tatctctttt ttgaaaagag tttttcctat cctcaacata aaaatctcgc
     8701 taattttctg cttatttgtt cgggaggaga gcgtgatgaa agtaagtaga tttctgggat
     8761 attgcagtat agtaacaggt gtgttttcgc cccttgcaca agcacaaccc aatatcatcg
     8821 tctataccac aaagatgtac cctattcaaa attcacaact tgcttcccag atttattatc
     8881 ttgaccaagt agagaatttt gaagagtcaa tcagtcgaaa cctcagtaaa tcaccagaga
     8941 ttgccgaaca agaaatgcgt gcattcttta attcagcaga ttggaaagcc caagagcaac
     9001 aattaaaaga cgcttatcaa ggcgtgatca gtggatggca aaatggcatt aagaaagtac
     9061 ctgcggtgtt ttttgataat ccacaatctt ctccgagtgt gatttatggt acgactgacg
     9121 tacagaaagc attagatgaa tggcataaat gggttaaaca acaaggtgaa tcaaaatgaa
     9181 aaaaacgttt cttacgatac tgatctctgc cagtttagct gtcccaacaa ccttatatcc
     9241 tcaaccagcc agagcctcat tgaataccgc ttccattgct gcatcaagtg cctcaacaag
     9301 ctgtttggaa tataaagttg ttggaacctg tttttggtta ttttgtacct actttggatg
     9361 taaggttcgc acatcaacca aaattaagca ttatttacct gaaatggttg tttcatctta
     9421 taaccacgat gggcaaacgc catggacaga attacaagct ctcaatcctg gtgtaaaagg
     9481 tggagaatac cgttctccac aacgccaata ctctcaactc actttcaaaa atgcggatgc
     9541 gtttggacac ccacaaggag ctatcggtca attattagga aatacggggt actattgtaa
     9601 aactcaaact acgccattta tgccttactt tttgagtggg ctggattttt ttgcctggcg
     9661 tgaaaatatg ccggaaatgt tttacccgga agctctgact ccgggaatgc gggaagttgg
     9721 ccgtgtaggc gatagctggg gaaatattta cccacgttcc ggttgggtaa cccaagcaca
     9781 cgattataaa gcctctgcga ccattgtgca gagagtggca gacattgtga gccgaacagc
     9841 tcagccacac atttatttcc cagcagccaa aacaggtggt gcaggagaat ggcctccagg
     9901 accggtcgaa gagggacaag caagaacaca taaatggcaa atactttacc ctcaaatgtc
     9961 aaatacctgc tctgtttttc ctaatggagg gcaaacagat acctattctt ctcaaatttc
    10021 aaatcaaggt gattatgctt gggcattatg gcgaccttat agttgttgca aacgccgtgg
    10081 gcaaacattc ctttacagta ttgattggtc acactaataa cattgggagc aataatgaaa
    10141 tctcacttta acttcaaaaa atcccactta cggatcacga cagcattatt atcaagtgta
    10201 tcaggactat cttatgccga ttcgattacg gataaactgg aatttagtaa gtcggattcg
    10261 attatttcag accgtgtttt ttaccaaatc ggcggtggaa tgggttatat ggctccacct
    10321 agtcgtggta ataccaaagt tgttgattta ggaattggtt ggaaagcgaa tttaacctgt
    10381 ggtaattttg atattaaaac gaccattaaa aatgatttaa acggtctcaa agacggcttt
    10441 aaagatttaa tggggaatgt cattgattca gcgaaaggtg cggttgcaag tatgcctgca
    10501 atggtattgc aacgggccaa ccctcaactc tacgatttac tcacaaatgg tatgtaccaa
    10561 ggtaagctgg atttcaaccg tcttaaaacc agttgtgaag aaatgtccaa agttttagca
    10621 gatcatacac tgggaggaaa atgggggcaa gcagctgatt tagaaagcta taagcaaatt
    10681 acctcctctc aacctgatgc aaaacaagct caaaaagacc tggaagataa aaaagggaaa
    10741 gatgggcgag tttggatagg aggtcagaaa aaaggtgggg aaaaccaaga agctatcaat
    10801 gttgttggcg atgttgtcaa agctgggttt aatttactga acaaacgtaa tgccttagat
    10861 accagtacaa tttcaggttc aagctgtgat gggaatcttt gccaaacttg ggataatcca
    10921 caaacagcag ccgatttttt aactaagatt attggtgaac aatctatttc gacttgtaaa
    10981 gaggattgtg gcactaaaac cagctcaaaa gccggtgtag gcttgagtcc ggaaattgag
    11041 gaagaacatg ttcgtactat tgtcaaatta gagaatgtac tgaatatgga tgtgcctaag
    11101 gctgaattgt tagctgattt gagttcaact actgtaccag taacaagagg gcttattgag
    11161 agtcttcgag atgatcctga tgttgaggta ttaagccaac gcttatctgc agaaattgcg
    11221 actagtaaga taatggaaaa aatgctcctg gctcggcgag cagtcttagc tggtatgcgt
    11281 gagccaaatg tcgctcaaaa tgcggatgca caagtagaac tggaaagagt actcaatatt
    11341 attgaccgtg aaatttccca agtaaaacta gaaatggata tgcaaaaaat gatcaccggc
    11401 aatacggctt cggtgatttt gcaaagcaaa tccgaacgag aagctcttgc tggtgctcat
    11461 agtttatctc aagacagtct ggataaacgt gttcaaggac ttgaaaacgg taatgttgca
    11521 ttaactgatt tagaggcagg ctctaatatt aacctctctc gtagaaatat tgcattgagt
    11581 atcccgaata ttccgaatgt agctgctctc atacctaatc tgccaaatcg aggtggttca
    11641 ggtgtaggtg gaaatttccc atcactcgca caaatcagcg gttcaacgat ggatcaagca
    11701 acaggattac tacgtaattt tgaaggcttt atctctaagg ctcaatggga tgtaaatgct
    11761 catcgagtag gatatggttc cgatacaatt acccgagctg atggtacgat cgtaaaagta
    11821 accaaagata cggtcattac caaagaagat gcagagcgtg atttggcccg ccgaacagct
    11881 atcttcgcaa ataatgtgag aaaagagctt ggagattcaa attggaatgc gttaccgccg
    11941 aacgctcaag ctgttttagt gtcttatgca tataattatg gttcacttgc taaaacaaaa
    12001 agcgtacttg atgcagccag aagatcagct caaagtggtg atatgaatgc tttagctact
    12061 gcaatcagaa atcgccaagt tgataataat ggcatcaatg cacgtcgccg gaatcaggaa
    12121 gctgactata ttcttggtaa aaattaacct cataaggagt tacatttatg cgtaaattat
    12181 taggtattac tgtacttaca ctttgtacaa cttcagcatt tgcaacggat aaaaatgtgg
    12241 tattttcttg taccagcaca gaaggtaaac ctctcacagt taaacgggta ggaaatgatt
    12301 acgagtattc gtatgataaa actatcttca aaaacccaat caaaaaagcg gtgaccaacg
    12361 atggctcaat tatcgctaga ggatcaggtt ttaccacgta tgcattagaa ctgaaaaatg
    12421 atggtttgaa gtatgtagtt gggtttgttc aaccaaatgg taactcaaaa gagtttattg
    12481 agcctggagc aacgattagc cgtgataatg atcaaccaag cattggctct gtagattgtg
    12541 atccttacaa aaaaatctat tataagtttg atgttcattt aatgaatacc ttgtaatgcg
    12601 ataattcaat aatatggggg aatggcttga tttttcatcc cccatactct attatataga
    12661 tgtggtgcta gagccagccc acgttaaaaa atacaaagct cggtagatga actattgtgg
    12721 tgccagagcc ggcccacgtt aaaaaataca aagctcggta gatgaggccc cttaagtcgt
    12781 aagatttaag gggttttgct taggatataa aatggatatt acaacacaaa atattgaact
    12841 tagatactta tctgagcagt tttaccttga tgtcgctcca catctgaaag aaattttgct
    12901 taaagagact cgcccatact gtgttctatt agttagagtt aaaaatttag attttgccct
    12961 accattgcgt tctaatttac ctgaaagaga aggagttgga attaaaacga tacctaatcc
    13021 tcaggaatac ggcaaattta aaggaattga tttctcaaaa gccattctac tgacagataa
    13081 aagctattta aaaacagata ccgttcagct caaaaacaaa tcagaattgt tgaatatcca
    13141 aggtaatgag cgtagaattg ttcgtgaatt taagaaatat gtgagtga
//
LOCUS       DANLZC010000066        13085 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.066, whole genome shotgun sequence.
ACCESSION   DANLZC010000066 DANLZC010000000
VERSION     DANLZC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.066"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            49..954
                     /locus_tag="PXE44_001453"
     CDS             49..954
                     /locus_tag="PXE44_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL5588961.1"
                     /translation="MSQKYSVAVIGLGAMGMGAAKSCIAAGLDTYGVDLNPNYLETLK
                     AAGAKKVGTSAVDFAAELDAVLLLVVNANQVNAVLFGENGLAQHLKQGTVVMVSSTIS
                     AQDAKNISQKLTDLGLVMLDAPVSGGAAKAAQGEMTVMASGSSEAFEKLKPVLDAVAG
                     KVYNIGEEVGLGATVKIIHQLLAGVHIAAGAEAMALAARAGIPLDLMYDVVTNAAGNS
                     WMFENRMKHVVEGDYTPLSMVDIFVKDLGLVNDTAKSLRFPLHLASTAYSMFTEASNA
                     GYGKEDDSAVIKIFSGIELPKKEGK"
     gene            956..2197
                     /locus_tag="PXE44_001454"
     CDS             956..2197
                     /locus_tag="PXE44_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005754893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL5588962.1"
                     /translation="MLGIIADDFTGASDIASFLTENGLKTVQMNGVPKTTLNSQVDAI
                     VISLKSRSNPVEEAIQQSLQALDWLKQNGCSQFYFKYCSTFDSTEKGNIGPVTDALMA
                     ALDTDFTVVTPALPVNGRTIFNGYLFVGDVLLNESGMRNHPITPMKDANLVRLMDAQS
                     QGKTGLVAYADVIQGAERVKARFAELKQQGYKYAVVDAADNSQLAVLAEAVADFQLVT
                     GGSGLGAYMAAYRSGGKKGTNAFVPSKGKTVVLSGSCSVMTNTQVAAYQQKAPSYFLE
                     AEKAIQDPNYVETLYQWVIANLDGDFAPMVYATVPPEKLKEIQTQFGGDKASHAIENT
                     FAKLAEKLQAAGVTNFITAGGETSSIVVQQLGFSGFHIGKQIAPGVPWLQAVEKPIFL
                     ALKSGNFGKEDFFSFAQEMFV"
     gene            2194..2826
                     /locus_tag="PXE44_001455"
     CDS             2194..2826
                     /locus_tag="PXE44_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDL5588963.1"
                     /translation="MTEQEQKIEMVNLARSLFERGYTVGGAGNLSVRLDENRILVTPT
                     GSSLGRLEADRLSVLDMQGNVLSGDKPSKESVFHLAMYQKNPACQAIVHLHSTYLTAL
                     SCLENLDTDNAMRAFTPYYVMRVGKLPVIPYYRPGDINIARELGERALDGKAFLLANH
                     GVVVTGSDLRDAVDNTEELEETSKLYFILQGQKVRYLTDDEVNDLKNRGK"
     gene            2828..3604
                     /locus_tag="PXE44_001456"
     CDS             2828..3604
                     /locus_tag="PXE44_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HDL5588964.1"
                     /translation="MPKFAANLTMMFNEVPFLDRFEAAANAGFKYVEYLWPYDYPAAE
                     LKAKLEQYGLKQVLFNTAAGDVAQGEWGVSAIPGREADSHRDIDLALEYALALGCENV
                     HVMAAVVPEGEDREAYKQTFIKNVQYAADKFKPHGINVLLEALSPEVKPNYLLKSQFD
                     TLEIVEAVNRDNVFVQLDYFHAQNVDGNLSRLTDKLKGKFAHIQIASVPDRHEPDEGE
                     INYQYIFNKLDEIGYEGYVGCEYKPRAETTSGLGWFEQYK"
     gene            3657..5015
                     /locus_tag="PXE44_001457"
     CDS             3657..5015
                     /locus_tag="PXE44_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntT protein"
                     /protein_id="HDL5588965.1"
                     /translation="MSNEMLILIGLASVVALLVIMIKGKVHPFLALGLVSIAVALTAG
                     IPMSKVIPTLISGMGSTLGSVALIVGLGAMLGKIIEKSHGADVLANWLLQRFGEKNAP
                     FALGMTGFIFGIPVFVDVGFIVLLPIIFSVARRLGGNILVYSLPIGLAMLLVHVLVPP
                     HPGIVAGAQNLNADIGFVLGIGLVAALPAYLIGQKFIPFFTKNNFVKVVTSTDLLQHQ
                     KDLIKEKGIELPSFGKVLAMISFPLILIMLGTVTATTLPKEHIVREVFSMVGSSPFAL
                     FVAVCLSAYVLGFRYGWTKHKLEEVLESALAPIAGIILITGAGGMFGKVLDASGVGKA
                     LADVLANTGLPLLVLSFILSALLRAAQGSATVATITTTTILAPAAVAAGYNDLQLALL
                     TAAIGAGSMTLSHVNDSLFWVWTKFFNVPVSVGLRTWTFLTTIFGVIAFAVVGIIWML
                     FV"
     gene            5077..6417
                     /locus_tag="PXE44_001458"
     CDS             5077..6417
                     /locus_tag="PXE44_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HDL5588966.1"
                     /translation="MLKKTLLFTLLLSHSLYAEDMIQENKSANITLLAEITAQGQKIN
                     GVALEYPDNVLSGSDLRQLYQVKTALDSAEKENRSVLKAYVNNEPKVTALPQAGKFVI
                     IELDTADKNAATYSVKDANKAPMKFKSKDENGNIVETEKVQAVKIPEFFANRLIYSVE
                     QKGLLKLTSGTTLGKTEWTQTAEPDKIKTPYLDDFVAKQVSLDQSGNKLNYRLYSPLQ
                     KNGEKYPLVIFLHGSGQVGSDNLAHLLSSKGAIATLPYAPSFVLAPQYDQVFDPFDNA
                     AKGQKGGIHWQTENRINLVLKMIDETLKANPQIDTTRIYINGLSRGAEGGLNLLLARP
                     NFFAGALLMSGREAYTNEWVDGNATKTNLAAIKHIPIWFFHSKEDKVSPVKGSRINYR
                     ILKDELNAPNVKYTEFTFEQAGDNGIVNNNPHNTWDAVYNSPEVIGWLLQQKRH"
     gene            6429..7118
                     /locus_tag="PXE44_001459"
     CDS             6429..7118
                     /locus_tag="PXE44_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005577085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDL5588967.1"
                     /translation="MFMYLSHPLDPKDLAWPGEPVVEVTRCTDVGEETPFSSFITKLP
                     NHCGTHMDAPRHFVKDGLSINELPIEYFCHKEVALIEIPKGEAEGITKEDLLPYVDIL
                     SKVSFAFLRTGFEKYRTENPAIYQNECPYISTGAGQYLSDTFPNLKGVGIDFLALGSP
                     SSRVPDAENPKNCHRAILGYYTGRFTTVIEDMHLAELPKNANIKQFFNAPWRIKGLDS
                     SQVTCIVELQQ"
     gene            7275..9092
                     /locus_tag="PXE44_001460"
     CDS             7275..9092
                     /locus_tag="PXE44_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDL5588968.1"
                     /translation="MIIKDTHWTSQQASPNAFPERQALMPIWGGKNIERNQWQSLWLE
                     QAPNALLQDGLAYFHIPFCASHCIFCGFYRNAWKENYSQIYTDRVISELRYESSLWRG
                     QGKIHAVYFGGGTPTALHTEDLVRLIRACYRYLPLAEDCEFTIEGRISHFDIDKAKAC
                     IEAGVNRISIGIQTFNSEIRKRLGRKHSGEEAQTYLKALSELNAVIVADLIFGLPNQT
                     DDIWQNDIEIATSLPISGLDIYAFNNYPFLPINRLIEHGKLPAPAGFDTQSLHYAYAV
                     EKLKAQGWQQVSNNHFAFPNRGERNRYNTLVKSNMPCLAFGSGAGGNFGGLSYQVQSD
                     LKTYLDSPLDQKSLSFLSKHGEHKALLGVIQHSMELGYLDTTPFEQNAKAEKLLQQWE
                     ALDLLKREKNQVYLNTSGRYWSPTLIRKLMLTLPQSSKDNNMITPLTKELENKLRTNL
                     KENPGQILEMLAGMNQCSLESVINCLPKEMVMQTGGERFVEIMQAIAGWNEAVTFIIH
                     TPDAIAEVTAKLPQGKIGRGFYNFEHGVDGGIHGHLRYENCAAIYLLDRPFMGKRTVA
                     LTFINQSGEAMFKIFVGRDEQGQLRENQIQAMRKLIGGC"
     gene            9095..9712
                     /locus_tag="PXE44_001461"
     CDS             9095..9712
                     /locus_tag="PXE44_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5588969.1"
                     /translation="MILVCGANGLLGRAICDKLPREKTIAVLRKDAMDTFFSERNIAV
                     EIADLLDLESCNALLQKTQPEILITVVGGKNEQGIRSDKTANINLIKAVEKYSPTTKV
                     VLVTSVGCAEQWDLISPLAQQALGEALKAKTEAEAYLQQTALNWLIIRPGGLNNDEVN
                     DEYQIVRDLPNERKMYVSRKSVATGIVRLLDSTENHQIFSIINRG"
     gene            complement(9796..10407)
                     /locus_tag="PXE44_001462"
     CDS             complement(9796..10407)
                     /locus_tag="PXE44_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentahemic C cytochrome"
                     /protein_id="HDL5588970.1"
                     /translation="MKSLSKIAFCFLAFIMPTFCFAGSNSYTTETIPLFADENLTQKI
                     GQLEAGAEIKLVQTTAKAAEIELNLWRKSKGFGRIWYHSFSKNITDAVFEKSFISNNP
                     LLEIVESKEDPNTGLVWQKVKMNVWVENAEFTQDLTNFWANTKNTYKTECSVCHKQRD
                     PKMHDANEWIAVFNGMVGFTDMDEATQKKVLRYLQMNAASSSQ"
     gene            10602..11510
                     /gene="glyQ"
                     /locus_tag="PXE44_001463"
     CDS             10602..11510
                     /gene="glyQ"
                     /locus_tag="PXE44_001463"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL5588971.1"
                     /translation="MSTKFNVKTFQGMILALQDYWASKGCTIVQPFDLEVGAGTSHPM
                     TCLRALGPEPMAFAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLDS
                     LKMLGFDPTQNDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPV
                     TGEITYGLERLAMYIQGVDSVYDLVWSDGELGKTTYGDVFHQNEVEQSTYNFEYADVD
                     FLFQCFEQYEKEAQYLLGLEKPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILR
                     IRALTKGVAEAYYASREALGFPGCKK"
     gene            11590..11850
                     /locus_tag="PXE44_001464"
     CDS             11590..11850
                     /locus_tag="PXE44_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588972.1"
                     /translation="MKPILYFADWCPDTAPFVAELDRLGIEYEPCDITKGGSTLKPFL
                     KLRDTHSAFDHAKANGYIGIPALLIEGEKVVLDLAELEGIFG"
     gene            11893..12927
                     /locus_tag="PXE44_001465"
     CDS             11893..12927
                     /locus_tag="PXE44_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL5588973.1"
                     /translation="MKTVIADNSAEFVNEISQLIQSSKQRMTVAVNAELTLLYWHIGK
                     RINDYILQGERAEYGQEVVKNLAQSLTEQFGKGWSKRHLNYTMQFATTFPNLEIVHAL
                     RAQLSWTHFKQIIHIDDPTKRGFYATMAAQERWSTRTLEDRIGSLLFERTAISKKPDE
                     TIIAELSLLREKGEYNQSMVLKDPYILDFLDLNDRYMERDLEDAILREIEQFLLELGA
                     GFTFVARQKRIQIDNDDFYIDLLFYNRKLKRLIAIELKTESFKHSHKSQMELYLAWLA
                     KYEQEADENPPLGMILCTGKKQEQVELLDLKNSDIHIAEYMTVLPSKAVLEQRLHLAV
                     ERAKERFLLK"
BASE COUNT         3883 a         2575 c         2960 g         3667 t
ORIGIN      
        1 gttaatatac cgccatcaag ttcattaatt tatttgaggg aataaacaat gagtcaaaaa
       61 tatagcgtag ccgtcatcgg cttaggagca atgggtatgg gagcagcaaa atcttgtatc
      121 gctgccggat tagacacata cggtgtggat cttaacccga attatctgga aaccttaaaa
      181 gcagcaggtg ctaagaaagt cggcacaagt gcggtcgatt ttgccgctga attagatgcg
      241 gtgttgttgt tggtggttaa tgccaaccaa gtgaatgcgg tgttattcgg tgaaaacggc
      301 ttagcccaac atttgaaaca aggtacggtg gtgatggtat cttccaccat ttctgcacaa
      361 gatgcaaaaa atatttcaca gaagttaaca gatttaggct tggtgatgtt agatgctccg
      421 gtttcgggtg gggcagcaaa agcagcacaa ggtgaaatga ctgtgatggc atcaggttcc
      481 agcgaggcat tcgagaaatt aaaaccggta ttagatgcgg ttgcaggcaa agtgtataac
      541 attggcgaag aagtcggctt gggtgcaacg gtaaaaatta tccatcaatt acttgccggt
      601 gtgcatattg ccgcaggtgc agaagcaatg gcattggcgg ctcgtgccgg tattccgctt
      661 gatttaatgt atgacgttgt aaccaatgcg gcaggcaatt cttggatgtt tgaaaaccga
      721 atgaaacacg ttgttgaggg cgattacacc ccactctcta tggtggatat ttttgtgaaa
      781 gatttgggat tggtgaacga caccgcaaaa tctttgcgtt tcccgcttca cttagccagc
      841 accgcttatt ctatgtttac cgaagccagc aacgcaggtt acggcaagga agatgatagt
      901 gcggtcatta aaattttcag tggtatcgaa ttacctaaaa aagaggggaa ataagatgtt
      961 aggcattatt gcagacgatt tcaccggtgc gagtgacatc gccagtttct taaccgaaaa
     1021 cgggctaaaa acggtgcaaa tgaatggtgt gccgaaaact acacttaaca gtcaagttga
     1081 tgcgattgtg attagcctga aatctcgctc taaccctgtg gaagaagcaa tccaacaatc
     1141 attacaagcc ttagattggc tgaaacaaaa tggttgctcg caattctact ttaaatattg
     1201 ttccactttt gacagcacgg agaaaggcaa tatcgggccg gtaaccgatg ccctaatggc
     1261 ggcattagat accgatttta ccgtagtcac accggctctg cctgtcaatg gcagaaccat
     1321 tttcaacggc tatttgtttg tgggcgatgt gctactgaat gaatctggta tgcgtaacca
     1381 cccgattacc ccgatgaaag atgccaactt ggtgcgttta atggatgcac aatctcaagg
     1441 caaaaccggc ttggttgcct atgctgatgt gattcaaggg gcagagcgtg tgaaagcccg
     1501 ttttgctgaa ttgaaacagc aaggctacaa atatgcggta gtagatgctg ccgataattc
     1561 acaacttgcg gtgcttgccg aagcggttgc cgattttcaa ttagtcacag gcggttcagg
     1621 cttaggggct tatatggcgg cttaccgttc aggcggcaaa aaaggcacga atgcgtttgt
     1681 accaagcaaa ggcaaaaccg ttgtgctttc gggttcttgc tcggtaatga ccaataccca
     1741 agtggcggct taccagcaaa aagccccaag ttatttctta gaagcggaaa aagccattca
     1801 ggatccgaac tatgtagaaa cgctttatca atgggtgatt gcaaacctag acggtgactt
     1861 tgccccaatg gtttatgcca ccgttccccc tgaaaaattg aaagagattc aaacccaatt
     1921 cggcggcgat aaagccagcc acgccattga aaataccttt gccaaattag ccgaaaaatt
     1981 acaagccgca ggcgtaacta actttattac cgcaggcggt gaaacttcga gcattgtggt
     2041 tcagcaactt ggattcagcg gtttccacat cggcaaacaa attgcaccgg gcgtgccgtg
     2101 gttacaagcg gtcgaaaaac cgatttttct tgcattgaaa tcgggtaact tcggtaaaga
     2161 agatttcttc agttttgctc aggagatgtt tgtatgacag aacaagaaca aaagattgaa
     2221 atggtcaatc ttgcccgctc gctgtttgag cggggctata ccgttggcgg tgcgggcaac
     2281 ctctccgtgc gtttagatga aaatcgcatt ttagttaccc caaccggctc ttcgctcggg
     2341 cgtttagagg cagaccgcct ttccgtattg gatatgcagg gtaatgttct ttcaggagat
     2401 aaaccttcaa aagaatcggt attccacttg gcgatgtatc agaaaaatcc ggcttgccaa
     2461 gcaatagtgc atctgcattc cacttattta accgcacttt cttgcttgga aaatttggat
     2521 accgacaacg ctatgcgtgc tttcacccct tactatgtga tgcgggtagg aaaattgccg
     2581 gtcattcctt actaccgtcc gggcgacatc aacattgccc gagagctggg ggaacgagcc
     2641 ttagacggca aagccttttt actggcaaat cacggggtgg tagtcacagg ttcagacttg
     2701 cgtgatgcgg tggataacac agaagagttg gaagaaacct ccaagctcta ttttattcta
     2761 caaggtcaaa aagtccgtta tctcaccgat gacgaagtca atgatctgaa aaacagagga
     2821 aaataaaatg cctaaatttg cagcaaacct tacgatgatg tttaacgaag tgccgttttt
     2881 agatcgtttt gaagcggctg ccaacgctgg atttaaatat gtcgagtatt tatggcctta
     2941 tgattatccg gcagcagaat tgaaagccaa acttgagcaa tatggcttaa aacaggtgtt
     3001 gttcaatacc gcagccggag atgttgctca aggtgaatgg ggtgtttctg ccattccggg
     3061 gcgtgaagct gatagccatc gtgatattga tttagcgttg gaatatgccc tcgccttagg
     3121 ttgtgagaat gttcacgtta tggcagcggt cgtgccagaa ggcgaagatc gtgaagccta
     3181 taaacaaacc tttatcaaaa acgtgcaata tgccgccgat aaattcaaac cgcacggcat
     3241 taacgtcttg cttgaagccc taagccctga agtgaaaccg aattatctgc ttaaaagcca
     3301 gtttgatacg ctagaaattg tggaagcggt aaaccgagac aatgtgtttg tacagcttga
     3361 ctacttccac gcacagaatg tggacggcaa cttatctcgc ctaaccgaca aactcaaggg
     3421 taaatttgcc cacattcaaa ttgcctctgt gccagatcgc cacgaaccgg acgaagggga
     3481 aattaactac caatatatct tcaacaaact cgatgaaatc gggtatgaag gctatgtagg
     3541 ttgcgagtat aaaccacgag cggaaaccac ctcaggacta ggttggtttg agcagtataa
     3601 ataaccacga tgattagcgg ataaatacgt gaatacaaca tttagaagga aactttatgt
     3661 caaatgaaat gttaattttg attggattag caagcgtagt tgctcttctg gtcattatga
     3721 ttaaaggtaa agtacaccca ttcttagcat tggggttagt gagtatcgca gttgccttaa
     3781 ctgcaggtat tccaatgagt aaagtgatcc caaccttgat ttcaggtatg ggaagtactt
     3841 taggtagcgt tgcactcata gtaggtttgg gagcaatgtt agggaaaatc attgaaaaat
     3901 ctcacggtgc ggatgtatta gcaaattggt tgttacagcg ttttggtgag aaaaacgctc
     3961 cctttgcatt aggtatgaca ggttttatat tcggtattcc tgttttcgtt gatgttggtt
     4021 ttatcgtatt gctcccgatt atttttagtg tagcgagacg tttaggggga aatatccttg
     4081 tttattcttt gccaatcggg cttgcgatgt tacttgttca cgttttagtt ccacctcatc
     4141 caggtattgt agcgggtgct caaaacttaa acgcagatat tggctttgta ttgggaattg
     4201 gcttagtagc agcgctacct gcatatttaa tcggtcaaaa atttattccg ttctttacaa
     4261 aaaataattt cgtaaaagta gtaacttcaa ccgatctttt acagcaccaa aaagatttga
     4321 ttaaagagaa aggcattgaa ctaccttctt ttggtaaagt gttagcaatg atttctttcc
     4381 cgctaatcct gattatgtta ggtacagtaa cggcaacaac cttgccgaaa gaacatatcg
     4441 tgcgtgaagt attcagtatg gtaggaagct ctccgtttgc cttatttgtg gctgtttgtc
     4501 tttctgctta tgtattaggt tttagatacg gttggactaa gcataaatta gaagaagtac
     4561 tggagtctgc gttagcaccg attgcgggga ttattttaat caccggtgca ggcggtatgt
     4621 tcggtaaagt gttagatgcc agtggcgtag gtaaagcatt agcagatgta ttagccaaca
     4681 cgggcttacc gttattggtt ctttctttca tactttctgc attattgcgt gcagcacaag
     4741 gttctgcaac ggtggcaact attactacta caactattct agctcctgca gctgtagcag
     4801 caggttataa tgatctgcaa ttagcacttc ttactgcagc tattggtgca ggttctatga
     4861 cattatccca cgtgaatgat tcactcttct gggtatggac gaaattcttc aatgttcctg
     4921 tatccgtagg cttaagaact tggacgtttt taacgactat tttcggtgta atagcctttg
     4981 ccgttgttgg cattatttgg atgttattcg tttaatatca ctctgttact gcttaaaatc
     5041 cacctcttat gaggtggatt tttttaggag aaacctatgc tgaaaaaaac acttttattt
     5101 acgctattac tttctcactc cctctatgca gaggatatga ttcaggaaaa taaatcagcg
     5161 aatattacgt tgcttgccga aatcaccgct caaggtcaaa aaattaacgg cgtggcattg
     5221 gaatatcctg ataatgtctt atcaggcagc gatcttcgcc aactgtatca ggtcaaaacg
     5281 gcattagata gtgcggagaa agaaaaccgt tcggtgctca aagcctatgt caataatgag
     5341 ccaaaagtga ccgcacttcc gcaagccggt aaatttgtga ttattgaatt ggatacggca
     5401 gataaaaatg cggcaactta tagcgtaaaa gatgctaata aagcgccgat gaaatttaaa
     5461 tccaaagatg aaaacggcaa cattgtagaa acagagaaag tacaagcggt caaaattcca
     5521 gagttttttg caaatcgcct aatctattcg gtggaacaaa aaggcttgct taaattaacc
     5581 agcggcacaa ctctaggcaa gactgaatgg acacaaaccg ctgaacccga caaaatcaaa
     5641 accccttatc tcgatgattt tgtcgcaaaa caagtgagct tagaccagtc tggaaataaa
     5701 ctgaactacc gtctctattc gcctttgcaa aaaaatggtg aaaaataccc gcttgtgatt
     5761 ttcctacacg gttcagggca agtcggttcg gataaccttg ctcacctgct ttccagtaaa
     5821 ggggcgattg ccaccctgcc ttatgcccca agttttgtac ttgctccgca atacgaccaa
     5881 gtattcgacc catttgataa tgccgccaaa gggcaaaaag gcggtattca ctggcaaacg
     5941 gaaaatcgta tcaatctcgt gttaaaaatg attgatgaaa ccctaaaagc caatcctcaa
     6001 attgatacca cacgcattta tatcaatggc ttatctcgag gtgctgaagg gggcttaaat
     6061 ctgttgttgg ctcgcccgaa tttctttgcc ggagcattat taatgagcgg tcgtgaagcc
     6121 tacaccaacg aatgggtgga cggcaatgcc accaaaacga atttggcggc aattaaacat
     6181 ataccgattt ggtttttcca tagtaaagag gacaaagtct cgccggtaaa aggttctcgc
     6241 attaactacc gcattctcaa agatgagtta aatgccccca atgtgaaata taccgaattc
     6301 acttttgaac aagccggcga caacggcatt gtcaataata accctcataa tacttgggac
     6361 gctgtgtata acagccctga ggtgattggg tggttacttc agcaaaaacg ccactaagga
     6421 ggctcactgt gtttatgtat ttgtctcacc cgttagatcc taaagattta gcttggccgg
     6481 gagaacctgt tgttgaggtg actcgctgca cagacgtagg tgaagaaacg ccatttagca
     6541 gttttatcac gaaactaccg aatcattgcg gcacacatat ggacgctcct cgccactttg
     6601 tgaaagacgg tttaagtatc aatgaattgc cgatagagta tttctgccat aaagaggtcg
     6661 ctttaattga aattcccaaa ggagaggcag aagggatcac caaagaagat ttattgcctt
     6721 atgtcgatat tctctccaag gtgtcttttg cctttttacg aacggggttt gagaaatata
     6781 gaaccgagaa ccctgctatt tatcaaaatg aatgccccta tatttcaacc ggtgccgggc
     6841 agtatttatc tgatactttc ccgaatttga aaggagtagg cattgatttc ttggcactag
     6901 ggtctccttc ttcccgagtg cctgatgcgg aaaaccctaa aaattgccat cgagcgattt
     6961 taggctacta taccggcagg tttaccactg tgattgaaga tatgcactta gcggaactcc
     7021 ctaaaaatgc caacattaag cagtttttta atgcaccgtg gcgaattaaa gggttagatt
     7081 ccagtcaagt gacttgtatt gtggaattac agcaataaac cacagatata ttcattttag
     7141 gagaggctat ttactatagt aagcataact caatcattaa ttgaaaataa ttattatttg
     7201 attttaatca agtcattcat aacttaattt tgttataaat atgaccttgt ttatagcact
     7261 attttgtttc gataatgatt ataaaagata cacactggac atctcaacaa gcctctccaa
     7321 atgctttccc agaacgacag gcacttatgc ctatttgggg tgggaaaaat attgaacgca
     7381 atcaatggca atctttatgg ctggaacaag caccaaatgc gttattacag gatggtttag
     7441 cttatttcca tattccgttt tgcgctagtc attgtatttt ttgtggtttt tatcgcaatg
     7501 cttggaagga aaattatagc cagatttata ctgatcgtgt gattagtgaa cttcgatatg
     7561 aatcttcact atggcgagga caaggtaaga ttcatgccgt ttattttggt ggaggaacgc
     7621 caacagcgtt acatacagaa gatttagtac gattgattcg agcttgttat cgttatttgc
     7681 cactcgctga agactgtgaa tttactattg agggtagaat tagccatttt gatatagata
     7741 aggctaaggc ttgtattgaa gcaggcgtga accgtatttc tattggtatt cagactttta
     7801 actccgaaat tcgcaaacgt ttagggcgca aacatagtgg cgaagaggct caaacttatt
     7861 taaaagcctt atcggaatta aatgctgtga ttgttgctga tttaattttt ggcttgccaa
     7921 accaaacaga tgatatctgg caaaacgata ttgaaattgc gactagttta ccgatctcag
     7981 gcttagatat ttacgccttt aataattatc ctttcctgcc tattaatcgc ttaattgaac
     8041 atggaaaatt acctgcgccc gcagggtttg acacccaaag cttacactat gcttatgcag
     8101 tagagaagtt aaaagcacaa ggttggcagc aagtgagcaa taaccacttt gcttttccaa
     8161 atcgaggtga acgcaatcgc tataatactt tggttaaatc gaatatgcct tgtttggcat
     8221 ttggctccgg tgcgggggga aattttggtg ggctgagtta ccaagtacaa tctgatttaa
     8281 agacctactt agattcgcct cttgatcaga aaagcctttc ttttttgagt aaacatggtg
     8341 aacataaagc actgcttggt gtgatacaac atagcatgga attgggttac ttagatacaa
     8401 cgccttttga acaaaatgcg aaagctgaaa aattattgca acaatgggaa gcgcttgatt
     8461 tactgaagcg tgaaaaaaat caagtatatc tcaataccag tggtcgttac tggtcaccaa
     8521 cattaattcg taaactaatg ttaacacttc ctcaatcaag taaggataac aacatgataa
     8581 cgccattaac taaagaatta gaaaataaat tgcggacaaa tttaaaagaa aacccagggc
     8641 aaattttaga aatgctggca ggaatgaatc aatgtagctt ggaatcggta attaactgtc
     8701 tgcccaaaga aatggtaatg caaacaggtg gtgagcgttt tgtggaaatt atgcaggcaa
     8761 ttgctggttg gaatgaagct gtgactttta ttattcatac gcctgatgcg attgcagagg
     8821 taactgctaa attgccacaa ggtaaaattg ggcgaggttt ttataatttt gaacatggtg
     8881 tagatggagg aattcacggt catttacgct atgaaaattg tgctgctatt tacttacttg
     8941 atcgcccatt tatgggaaaa agaacggtgg cgttaacttt tatcaatcaa agcggtgagg
     9001 cgatgtttaa gatttttgtt gggcgtgatg aacaaggtca attacgagaa aatcagattc
     9061 aagccatgcg gaaattaatt ggaggatgct agtcatgatt ttggtttgtg gggcaaatgg
     9121 gttgttagga cgagcaatct gtgacaaatt acctagagag aaaacgatag ccgtcttacg
     9181 aaaagatgct atggatacgt ttttcagcga acgaaatata gcagtagaaa tagcagattt
     9241 attagatttg gaatcctgca acgccttgtt acaaaaaaca caaccggaaa tcttgattac
     9301 ggtagtagga ggaaaaaatg aacaaggtat tcgtagcgac aaaacagcaa atattaattt
     9361 gataaaagca gttgaaaaat attcaccaac tacgaaagta gttttagtta ctagtgttgg
     9421 gtgtgcagaa caatgggatt taattagccc attagcacag caagctttag gtgaagcact
     9481 aaaagccaag acggaagctg aagcttattt acagcaaaca gcattaaatt ggctgattat
     9541 tcgacccggt gggttaaata atgatgaagt aaatgatgag tatcagattg tgcgtgattt
     9601 acctaatgaa cggaaaatgt atgtaagccg taaatctgtc gcaacaggca tagtgcgttt
     9661 actggatagt acggaaaatc atcaaatctt ctctattatt aataggggat agtatatttc
     9721 aacgaaggca agcgggcata ttttatggtt attttgcaac tataaaacag accgcttgtg
     9781 agatcttttt ctattttatt gagaactgga tgccgcattc atctgcaagt agcgaagcac
     9841 ctttttctgg gtagcttcgt ccatatcggt aaaaccgacc attccgttaa atactgcaat
     9901 ccattcgttt gcatcatgca ttttaggatc acgttgttta tggcacacgc tgcattctgt
     9961 tttataggta tttttggtgt tcgcccaaaa gttagtgaga tcttgggtaa attcggcatt
    10021 ttccacccac acgttcattt tcactttttg ccataccaag ccggtgttcg gatcttcctt
    10081 gctctctaca atctctaaca gtgggttgtt gctgataaaa gatttctcaa ataccgcatc
    10141 ggtaatattt ttactgaaag aatgatacca aatccgcccg aaacctttgc tttttcgcca
    10201 taaattcagc tcaatttcag cggcttttgc ggttgtttgc accaatttaa tttctgctcc
    10261 ggcttctagc tgcccgattt tctgtgtcaa attttcatcg gcaaataaag ggattgtttc
    10321 cgtggtgtaa ctgtttgaac cggcaaaaca aaatgttggc atgatgaatg ccaagaaaca
    10381 gaaagcgatt ttcgataatg atttcatcat aaaccccaca aattagatga gaaattaggt
    10441 tatatgttac acaaattgag caaaaaagcg ttgatttaca tcataaaact gattttatgt
    10501 cgatttctta attttttgca aaaaaatccc aaaaaatgac tgcttacaag ctataataac
    10561 cagtattttt cgaccatttt tcaaattcaa gagaaaataa aatgagtaca aaattcaacg
    10621 taaaaacctt tcaaggaatg attttagccc tgcaagacta ctgggcaagc aaaggttgca
    10681 ccattgtgca gccgttcgat ttagaagtgg gggcaggcac ctcgcaccca atgacctgcc
    10741 tgcgtgcgct tggccctgag ccaatggcgt ttgcctatgt gcaaccatct cgccgcccga
    10801 cagacggtcg ctacggcgaa aatccgaacc gtttacagca ctactaccag ttccaagtgg
    10861 tgattaaacc ttcgccggat aacattcaag aactttacct cgacagcctg aaaatgctcg
    10921 gtttcgaccc aacccaaaac gacattcgtt ttgtggaaga caactgggaa aacccaacgc
    10981 ttggggcgtg gggcttaggt tgggaagtgt ggctgaacgg aatggaagtg acccaattca
    11041 cctacttcca gcaagtcggc ggcttggaat gtaaaccggt gacaggcgaa atcacctacg
    11101 gcttagagcg tttggcgatg tacattcaag gcgtggacag cgtgtacgac ctcgtgtggt
    11161 ctgacggcga actcggtaaa accacctacg gcgacgtatt ccaccaaaac gaagtggagc
    11221 aatccaccta caactttgaa tacgctgatg tggacttcct gttccaatgc ttcgagcagt
    11281 acgaaaaaga agcccaatac ctgttaggcc tagaaaaacc attgccattg ccggcatacg
    11341 aacgcatttt aaaagccgct cacagcttta acttattaga tgctcgtaaa gcgatctcgg
    11401 taactgaacg ccaacgctac attctacgca ttcgtgcctt aaccaaaggc gttgctgaag
    11461 cgtactatgc cagccgtgaa gcgttggggt tcccggggtg taaaaaataa tcgtagggac
    11521 gccacgcttg gcgtccgtca ataaattaca aaacggacac gaagcatcgt gtccctacaa
    11581 ggatttacga tgaaaccgat tttatacttc gccgattggt gtccggatac cgccccattt
    11641 gtggcagaac tagaccgctt gggcattgaa tatgagccgt gcgacatcac caaaggaggc
    11701 tcaacactta aaccattttt gaaactgcga gacacgcatt ctgcctttga ccacgccaaa
    11761 gccaacggct atattggcat tccggcattg ctgatcgaag gcgaaaaagt cgtgctggat
    11821 ttggctgaat tagagggtat tttcggctaa caagcggttg aatttggcgg aaatgttgca
    11881 aaggagccaa taatgaagac agtaatagca gataattcgg ctgaatttgt gaatgaaatt
    11941 tcacaactta ttcaatcgag taagcaacga atgaccgttg cggttaatgc agaattaacc
    12001 ttgctttatt ggcatatcgg aaaacggatc aatgactata ttttgcaggg tgaacgtgcg
    12061 gaatatgggc aagaagtggt gaaaaatctt gctcaaagtc tgacggagca atttggtaaa
    12121 ggttggagta aacgccattt aaattataca atgcaatttg ctacaacttt ccctaatttg
    12181 gaaattgtgc acgcactgcg tgcacaatta agctggacac attttaagca gattattcat
    12241 attgatgatc caacaaagcg agggttctat gcaacaatgg ctgcacagga gcgttggagt
    12301 acaaggacat tagaagatcg cattggctca ttattgtttg aacgcacggc aatttccaaa
    12361 aagcccgatg aaaccattat tgctgaattg agtttgctac gtgaaaaagg cgaatataat
    12421 cagtcaatgg tgttgaaaga tccctatatt ttggattttc tcgaccttaa cgaccgttat
    12481 atggaacgtg atctcgaaga tgcgattcta cgagagatcg aacagttttt actggaactt
    12541 ggggcgggtt ttacctttgt cgcccgccaa aaaaggattc agattgataa tgatgatttc
    12601 tatatcgatc tgctgtttta taaccgtaaa ctcaaacgcc tgattgcgat tgaactaaaa
    12661 accgaaagct tcaaacattc ccacaaaagt caaatggaac tctatttggc gtggctggcg
    12721 aaatacgagc aagaagcaga cgaaaaccca ccgcttggaa tgattctctg caccggtaaa
    12781 aagcaggagc aagtggaatt gttggatttg aaaaattccg atattcatat tgcggaatat
    12841 atgaccgtgt tgccgagtaa agcggtattg gaacaacggt tgcatttggc ggtggaaagg
    12901 gcgaaagagc ggtttttact aaaatagcaa gcggttaaaa tcagcggaaa tgttgcaaaa
    12961 tgaaagtatg atggaaacga tttggttgca tattccgttg gtgggtggtt agagattttc
    13021 aaatctcccc cctccccctc tttggctaaa gagggggcga tcatttagtg agtaatttta
    13081 attca
//
LOCUS       DANLZC010000067        12924 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.067, whole genome shotgun sequence.
ACCESSION   DANLZC010000067 DANLZC010000000
VERSION     DANLZC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12924
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.067"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..794)
                     /locus_tag="PXE44_001466"
     CDS             complement(<1..794)
                     /locus_tag="PXE44_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determination protein"
                     /protein_id="HDL5588974.1"
                     /translation="MADSVKSHEQSSDEIDLIEIFRALWDKKWWIVITTFITTLLAGI
                     YAFTAKEQWTSKATVIAPKLTELGEYLNVRREYSRILRVDPVDPNALSASLFNNFNRL
                     AQSQDTKNEFFITSNVYKTLAEGKDEQGQRTVLSNLSNENTSFIIPDAKKDPNAIGRT
                     LSFSAETPMEAQDTLGKLVQFINSKAFAVELEDFLVDFQNVLTALQYEKSLIQEDLSV
                     NKSVQLENLNKAYETAQKAGIKEYSKAFNQSDASNIAAALSDTKISL"
     gene            943..1284
                     /gene="erpA"
                     /locus_tag="PXE44_001467"
     CDS             943..1284
                     /gene="erpA"
                     /locus_tag="PXE44_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDL5588975.1"
                     /translation="MSDIQIPLIFTDAAARKVKSLIEGEENPNLRLRVYITGGGCSGF
                     QYGFTFDDQVNEGDLTIENQSVGLVVDPMSLQYLIGGTVDYTEGLDGSRFVVNNPNAS
                     STCGCGSSFSI"
     gene            complement(1389..1712)
                     /gene="yidD"
                     /locus_tag="PXE44_001468"
     CDS             complement(1389..1712)
                     /gene="yidD"
                     /locus_tag="PXE44_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL5588976.1"
                     /translation="MVKTHSPEQKAGTTESINQPPRLSLLAKILLLPIYFYRYAISPL
                     IGPRCRFYPTCSTYAVEAIKIHGAIKGGYLAFKRILRCNPLSEGGEDPVPLCCKGNCK
                     HNDKK"
     gene            complement(1661..2035)
                     /gene="rnpA"
                     /locus_tag="PXE44_001469"
     CDS             complement(1661..2035)
                     /gene="rnpA"
                     /locus_tag="PXE44_001469"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL5588977.1"
                     /translation="MKKLNFSRELRLLAPVQFKAVFEEPLRASTPQMTLLARENNLEN
                     PRLGLTVAKKHLKRAHDRNRIKRIVRESFRLKQHELPNFDFVFVAKGGIGKLDNATLF
                     ETLEKLWSRHIRLSKKPAQQNP"
     gene            complement(2071..2205)
                     /gene="rpmH"
                     /locus_tag="PXE44_001470"
     CDS             complement(2071..2205)
                     /gene="rpmH"
                     /locus_tag="PXE44_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL5588978.1"
                     /translation="MKRTFQPSVLKRARTHGFRARMATKNGRQVLARRRAKGRKSLSA
                     "
     gene            2442..3404
                     /gene="hemH"
                     /locus_tag="PXE44_001471"
     CDS             2442..3404
                     /gene="hemH"
                     /locus_tag="PXE44_001471"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDL5588979.1"
                     /translation="MNQPQQIGILLANLGTPDEPTPSAVKRYLKQFLSDPRVIDLPKF
                     KWQAILNCIILPRRSPKVAKLYQSVWSEEGSPLLAISRQQQRAVQRYFDAKGKNVVVE
                     LGMSYGNPSIESATDNLIKQGVERIIVLPLYPQYSSTTTASVFDAFANGLKAQRKIVP
                     FDFIHSYHNDPLYIQALANSIDVKPDELLLFSFHGIPKRYETEGDFYAKHCQETAELV
                     AEKLGLPREKWYLSFQSRFGDEEWLQPYTDQTLESFPQKGIKKVAVVCPGFSADCLET
                     LEEITEENRENFEHAGGESYRYIPALNASGDHIKALVKLIEAKI"
     gene            complement(3468..4415)
                     /locus_tag="PXE44_001472"
     CDS             complement(3468..4415)
                     /locus_tag="PXE44_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL5588980.1"
                     /translation="MPTKPHKEYHELVELLISRGMLINDKSYAEKKLSQVGYYRLSGF
                     WHISRKPNQDGGLSENFLENVQFEEVYKLYIFDKKLRLLLLDIIERLEVNIRNIMAHE
                     LGRENPLAYEDYSLINQKFLKFQWKSNYAYANWLETTANKIKRSKDDSIEWHRKNEKE
                     IPFWVITETWDFGALSKFYSLLNGQHQRKIAKKFNVDENTLKNWLNEINIVRNLSAHH
                     SRIWNRKFSDIAIPKLDDPRWSKFKKMQQYFSNLSLDDKAKERIFGRVAILWFLVSQT
                     STNYHWLDKFEDLLNDFPNLPNTKVELMGFNREILEKIK"
     gene            complement(4627..6744)
                     /gene="spoT"
                     /locus_tag="PXE44_001473"
     CDS             complement(4627..6744)
                     /gene="spoT"
                     /locus_tag="PXE44_001473"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL5588981.1"
                     /translation="MYLFEPLDRIIQGYLPPEQIERVKRAYVIARDAHEGQTRSSGEP
                     YITHPVAVASIIAEMKLDQEAVIAALLHDVIEDTPYTEEELAAEFGQSVADIVQGVSK
                     LDKLKFRTRQEAEVANFRKMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAK
                     ETLEIYSPLAHRLGIEHIKNELEDLGFEAMHPQRYRVLKMAIASARGHRKELIQRIAD
                     DIRGRLEEVQIEARVYGREKHLYSIYQKMRQKDQHFHSILDIYAFRVVVDNVDNCYRV
                     LGQMHALYKPRPKRVKDYIAVPKPNGYQSLHTSMIGPHGVPVEVQIRTEDMDKMADMG
                     VTAHWRYKEGESHHNTTVQLKAQQWLQSIVELQNSAGNTAEFIDSVKSDLFSDDIFVF
                     TPKGRIVELPANATAIDFAYAVHSGIGDRCVGAMVDRNPYPLSEPLRTGQTVEIITEQ
                     GKRPNASWLNFVVSGKARAKIRQSLKNQQHDEAIALGRRQLVRAMNLRSLDELCSETI
                     QSVLDELRLSSFDELLAEIGLGNQMSNVIAQRLLGEPLLIDTDGNPENNQPLKIEGIE
                     NRLISFANCCLPIPGDEIIAHVSSGKGLAIHNACCGNVKDYATEPSNYLPVEWEIAKE
                     KAIQFETEIVLDIINEPGVLAGITSTLAKLNSNIGSINSEAREGNIYQVRVQISVENK
                     EHSELILRKLLSVKGVVKAARKQ"
     gene            complement(6854..7135)
                     /gene="rpoZ"
                     /locus_tag="PXE44_001474"
     CDS             complement(6854..7135)
                     /gene="rpoZ"
                     /locus_tag="PXE44_001474"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL5588982.1"
                     /translation="MARVTVQEAAEKIGNRFDLILIAARRARQLQLHTREPLVPEEND
                     KPTVIALREIEEGLITTDIMDQLENHDSIKQEAAEQEAISFLTEMSNEA"
     gene            complement(7238..7735)
                     /locus_tag="PXE44_001475"
     CDS             complement(7238..7735)
                     /locus_tag="PXE44_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL5588983.1"
                     /translation="MAHFYEYLEFNSDEDRENQLEVYVEVKLSTDTEEKLQAVGVQGD
                     WLVMAEPYCPDCVEIVAYFQRMAKLNPNIKVKYVARKDNQERKHFDSDEQQQAVISAQ
                     KIPTIFDIRNGKTELVLSEFPQFLKDKMAANPEQFEQLKADFRMGKFGKEVEAELLAI
                     LLNTK"
     gene            complement(7760..8782)
                     /locus_tag="PXE44_001476"
     CDS             complement(7760..8782)
                     /locus_tag="PXE44_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5588984.1"
                     /translation="MSVTKKRLTLSDIAELSGVSKTTVSMILNGRGNDFRIKSETCQK
                     VKEIAKKHGYRANVYAKSLQAQRTNVIGLVIPDLTNYGFALTSRTLEKLCRENGLQLV
                     IACSDDTFEGEKKAIERLLDRQVDLLITAPTHNEPTYYQNAAQYTPMLQLDRYISGLN
                     IPSITSNDTQSISMLVENIVRAYHLKEFFYLGGQLSLSPSQNRLKGFREGLEQSHLAL
                     DENWVLHQDYQPESGYQMFAQIVEKLGRLPEAVFTASFTLLEGVMRYLTEHQQMDKLL
                     TQELHLATFDDHHLLNALPFHIHSIKQDHQQIAERTFELIQQKLAQQKIDDVKVDCEI
                     LWRKSI"
     gene            complement(8822..10252)
                     /locus_tag="PXE44_001477"
     CDS             complement(8822..10252)
                     /locus_tag="PXE44_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL5588985.1"
                     /translation="MHIFNNGKYKSLHAQSEGELNALRQTVLSDPDFYPTYHLAPETG
                     LFNDPNGLIFDGEKYHIFAQWFPYGALHGMKHWQHFITTDFQTFSKGDLLIPDELFES
                     HGCYSGGAILWQDKIVAFYTGNTRRPSDNARIPHQNIAIFDKSGKLLEKRCIIDQAPA
                     GYTEHVRDPKPYLTAEGKIRFVLGAQRENLTGTALIYEMDDLNATPRLIAELEVHNFD
                     NSNVFMWECPDLFSLGGKDLFVWSPQGKLRESHQFQNNYHATYALGELNGNRLRAEHI
                     EELDYGFDFYAPQTVENSDRILFGWVGLPDLTYPTDKYQWHSILTLPRKLSLQNGKVI
                     QNPIVNVGELQAVEMSENFAIENLDTAYLQISVENQPLVLDFFSNEQGQKLSLRFENG
                     VLSLDRSQTEQTELMEKFGSVRHCKIEQLETIEIFFDRSVVEIFVNGGEKVLTSRFFI
                     AKRENVVKSSRPIQVKVAKVLPISVE"
     gene            complement(10357..11280)
                     /locus_tag="PXE44_001478"
     CDS             complement(10357..11280)
                     /locus_tag="PXE44_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDL5588986.1"
                     /translation="MSKIWVTGDAVVDLIPDGEHHYLKCAGGAPANVAVGVSRLGVEA
                     GFIGRVGNDPLGKFMREVLQAENVCTKQMILDAQHRTSTVIVGLDNGERSFTFMVNPS
                     ADQFLEIGDLPEFNQGDFLHCCSIALINDPSRSTTIEGIRRVKKAGGYVSFDPNLRES
                     LWKSLDEMKTVVNSVVAMADILKFSEEELTLLTETETLEQATQAITAQYPEKLIIITL
                     GKDGAIYHFNGKSQVVAGKALKPVDTTGAGDAFVSGLLAGLAQTPNWHDETVLVEVIR
                     KANASGALATTAKGAMSALPNKAELDAFLAE"
     gene            complement(11397..12824)
                     /gene="miaB"
                     /locus_tag="PXE44_001479"
     CDS             complement(11397..12824)
                     /gene="miaB"
                     /locus_tag="PXE44_001479"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDL5588987.1"
                     /translation="MAKLHITTWGCQMNEYDSSKMADLLNSTHGFELTENPEDADVLL
                     LNTCSIREKAQEKVFSQLGRWKNWKKDKPNLIIGVGGCVASQEGEHIRDRAPYVDIVF
                     GPQTLHRLPEMINQIRGGKSSIVDISFPEIEKFDRLPEPKAEGPTAFVSIMEGCNKYC
                     SFCVVPYTRGEEVSRPVDDVLFEIAQLAAQGVREVNLLGQNVNAYRGETFDGGICTFA
                     ELLRLVAAIDGIDRLRYTTSHPIEFTDDIIEVYRDTPELVSFLHLPIQSGSDRVLTMM
                     KRNHTALEYKAIIRKLRAVRPEIQISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFS
                     FIYSARPGTPAADLPDNVSDDEKKERLSRLQQRINHQAMQFSRAMLGTEQRILVEGPS
                     KKDLMELTGRTETNRVVNFVGTPDMIGKFVDVKITDVYTNSLRGEVIRTEDQMGLRVV
                     ESAESVIARTRKEDDLGVGKYVVNL"
BASE COUNT         3681 a         2787 c         2479 g         3977 t
ORIGIN      
        1 agggagattt tagtatcact taaagctgct gcaatatttg atgcatctga ttgattaaat
       61 gcttttgaat attctttaat acctgctttt tgtgcagttt cataggcttt atttaagttt
      121 tccaactgaa ccgacttatt cacagataaa tcttcctgaa ttaatgattt ttcatattgt
      181 aatgcagtaa gcacattttg gaaatcgact agaaaatctt ctaattcaac agcaaatgct
      241 ttgctattaa tgaattgaac caacttacca agggtgtcct gcgcctccat aggtgtctct
      301 gcagaaaaag atagtgtacg acctattgca ttaggatctt tctttgcatc aggaataata
      361 aagctggtat tttcattact caaattggat aaaactgtgc gttgcccctg ctcatctttt
      421 ccttcagcta acgtcttata cacattggaa gtgataaaaa attcattttt agtatcttga
      481 gattgtgcta atcggttaaa attgttgaac aaactcgcag acaatgcatt tggatcaaca
      541 ggatctacac gcaaaatccg actatattct ctgcgaacat ttaaatactc acctaactct
      601 gttaatttag gggcaataac tgttgcctta gacgtccatt gctcttttgc agtaaatgca
      661 taaataccag caagtaatgt ggtaatgaat gttgttataa caatccacca ttttttatcc
      721 catagagcac gaaaaatctc aattaaatca atctcatccg agctttgctc atgacttttt
      781 actgaatctg ccatagtttc ctctaaaatt tgcatgtttc tgtacagcat tatacataat
      841 tttcttattt ccactgcatt catttactct tttttaagat tgcacttcct atgtaattat
      901 tagataatat ccaatcacag taaaattttg atctaaaaca aaatgagcga tattcaaatt
      961 cctttaatct ttactgatgc ggcggctcgt aaagtaaaaa gcttaattga gggcgaagag
     1021 aacccgaatc ttcgcttgcg tgtgtatatt acgggtgggg gctgtagtgg attccagtat
     1081 gggttcacct ttgatgatca agtcaatgag ggagacctta ccattgagaa tcaaagcgtt
     1141 ggcttggtgg ttgacccgat gagcctacaa tatttaattg gtggcacggt ggattatacc
     1201 gaagggctgg acggctcacg ttttgtcgtg aataatccaa atgcaagctc aacctgtggt
     1261 tgtggttctt cctttagcat ttaattcact cgaattattg ccttattcaa tatcagtctc
     1321 acaagtaaat tttttactaa aaatgaccgt ttgtattaaa caaaatagcc taacttattg
     1381 agtttaggct attttttatc attatgtttg cagttgcctt tacaacaaag tgggacagga
     1441 tcttcgcctc cctcactcaa agggttgcaa cgcagaatac gcttaaacgc taaataccca
     1501 cctttaattg ctccgtggat tttaatcgct tcgaccgcat aggtggagca ggttgggtaa
     1561 aatcggcaac gtgggccaat caatgggctg atagcatagc gatagaaata aatcggtagc
     1621 aatagaattt tggctaatag gctgagacga ggtggctgat ttatggattc tgttgtgccg
     1681 gctttttgct caggcgaatg tgtcttgacc atagtttttc caatgtttca aacagcgttg
     1741 cgttgtctaa tttgccaatg ccgccttttg ccacaaatac aaaatcgaaa ttcggcaatt
     1801 cgtgttgctt gaggcgaaaa ctttcacgca ctatccgctt aatgcgatta cggtcgtgag
     1861 cccgttttaa atgttttttg gcaacagtta agcctagacg aggattttct agattattct
     1921 ctctagcaag aagagtcatt tggggtgtgc tggctcgaag tggctcttca aacaccgcct
     1981 taaattgaac gggagctaac aaacgtagct cccgagagaa atttagcttc ttcacactta
     2041 atcagaaagg tgttttggct ggttctgcaa ttatgcagat aaagatttac gacctttagc
     2101 acgacgacgc gctaacactt gacgaccgtt tttagtagcc atacgagcac ggaaaccgtg
     2161 agtacgagca cgttttaata ctgatggttg aaatgtacgt ttcattgctt atctacctaa
     2221 aatttaaaaa gttggcataa aatggtttct gccaaacaag gaccataaaa aagagttgca
     2281 attctacagg caattaaaat aaaaagcaag aaacaatcca agcacaactc gggttgtttg
     2341 caaaattttt gaaaaatacg accgcttgtt ataatcgcag aaatttatat tggtttgtgg
     2401 cataatcgaa aaaatattaa ctattgatga gaaatagaac aatgaatcaa ccccaacaaa
     2461 taggtatttt gctggctaat ttaggtacgc cagatgaacc cacgccaagt gcggtgaagc
     2521 gttatttaaa gcaattttta agcgatcctc gtgtgatcga tctaccaaaa tttaaatggc
     2581 aagcaatcct aaactgcatc attttgccaa gacgttcgcc aaaagtggca aaattatatc
     2641 aatcagtatg gagcgaagaa ggctcaccgt tattggcgat ttctcggcaa caacaacgag
     2701 cggtacaacg ctattttgat gccaaaggca aaaatgtggt ggtggaattg ggaatgtctt
     2761 acggcaatcc aagtattgaa agtgcgaccg ataacctcat caagcagggg gttgagcgga
     2821 ttattgtgct accgctttat ccgcaataca gtagcactac taccgcttcg gtgtttgatg
     2881 cgtttgccaa cggtttaaaa gcacagcgta aaatcgtgcc gtttgatttt attcacagct
     2941 atcataacga tccgctttat attcaagcgt tggctaactc tattgatgtt aaacctgacg
     3001 agttattact gttttcattt cacggcattc ctaaacgtta tgaaacggaa ggtgattttt
     3061 acgccaagca ttgccaagaa acagcagaat tagtggctga aaagctaggt ctgccaagag
     3121 aaaaatggta tttgtctttc cagtcccgtt ttggtgatga agagtggcta cagccttata
     3181 ccgatcaaac ccttgaaagc ttcccacaaa aagggataaa aaaagtggct gtcgtttgcc
     3241 cgggcttttc agcagattgt ttggaaacct tagaagaaat tacagaagaa aaccgcgaga
     3301 actttgaaca tgccggcgga gaatcttatc gctacattcc agcgttaaat gcgagtggcg
     3361 atcatatcaa ggcattagta aaattaatcg aagcgaaaat ataatataat ttgggcggat
     3421 aataaatccg cccattttta tctacaccct ataggaatgt acttatttta tttaattttc
     3481 tctaatattt ctctgttaaa tcccatcagt tctacttttg tattcggtaa attagggaag
     3541 tcatttaata aatcttcaaa cttatctaac caatggtaat tagtgctggt ttgactaact
     3601 aaaaaccaca gaatagctac ccttccgaaa attctctcct ttgctttgtc atccagcgat
     3661 aagtttgaaa aatactgctg cattttttta aatttcgacc aacgaggatc atctagctta
     3721 ggaatagcaa tatcgctaaa ttttctgttc caaatacgag aatggtgagc agaaaggttt
     3781 cttacaatgt ttatttcatt caaccaattc tttaacgtat tctcatctac attaaatttc
     3841 ttagcaattt tcctttggtg ttgcccattt aacaatgagt aaaacttaga taatgcacca
     3901 aaatcccagg tttcagtaat tacccaaaaa gggatttcct tctcattttt tctatgccat
     3961 tcaatagaat catctttact acgcttaatt ttattagctg tagtttctag ccaatttgca
     4021 taagcataat tagatttcca ttgaaatttt aagaactttt gattaatcag agaataatct
     4081 tcataagcca aaggattttc acgccccaat tcatgagcca taatattacg aatattaact
     4141 tctaaacgtt caataatatc caataataaa agccttagtt ttttatcaaa aatatatagc
     4201 ttataaactt cttcaaattg tacattttcg agaaaatttt cgctaagccc accatcttga
     4261 ttaggctttc tagatatatg ccaaaatcca gataaacgat aataccctac ctgagataat
     4321 tttttctctg catagctttt atcatttatg agcattcctc tagatatgag tagttctact
     4381 aattcgtgat actctttatg tggttttgta ggcataaaat tgaaggcatt ttaagaaagg
     4441 caaggacgta gcttgacact ctaagtcgat tacatttagt aaacctcagg gacaggggct
     4501 acgccacgat tgcaagggat tatacatttt agctgattaa agtcaaaagg tttcatcaat
     4561 taataatagc aatactgtac ttttaaggac tataattgca aaaaactgat aaaattttac
     4621 cgcttgttac tgcttacggg ccgcttttac aacacctttt acacttaata gcttacggag
     4681 aatcagctca gaatgctctt tattttccac agaaatttgt actctcactt gatagatatt
     4741 tccttctctt gcctcgctat taatgctgcc aatgttgcta tttaatttgg caagcgttga
     4801 tgtgatcccc gcgagtaccc ctggttcatt gataatatcc agtacgattt ccgtttcaaa
     4861 ttgaatcgct ttttctttgg caatttccca ttccacaggt aaataattac tcggctctgt
     4921 tgcgtaatct ttcacattac cacaacaagc attgtggatt gccaatcctt tacctgaact
     4981 gacgtgagca ataatttcat cacccggtat aggtaagcaa cagtttgcaa aactgataag
     5041 gcggttctca atcccttcaa ttttaagcgg ttgattattt tctggattac catcagtatc
     5101 aattaatagt ggctccccta ataagcgttg agcgataaca ttactcattt ggttacctag
     5161 accaatttca gccagtagct catcaaagct tgataaacgt agctcgtcta atacggattg
     5221 aatagtttct gaacacaatt catcaagtga gcgtaaattc attgcccgaa ctagctgtct
     5281 acgcccaagt gcaatcgcct catcgtgctg ctgattttta agcgattgac gaattttagc
     5341 cctcgcctta ccacttacta caaaatttaa ccacgatgca tttgggcgct taccttgctc
     5401 ggtaataatt tccaccgtct gtcctgttcg tagcggttct gaaagtggat acggattgcg
     5461 atcaaccatc gcacctacac aacgatcccc aatgcctgaa tgcaccgcat aggcaaagtc
     5521 aatagcggta gcattcgccg gcaattcaac aatacgccct ttcggggtaa agacgaaaat
     5581 atcgtcagag aacagatccg atttaacgct atcaataaat tctgctgtat tgccggcact
     5641 gttttgcaat tcaacaatac tttgtagcca ttgctgggct ttgagttgaa cggtcgtgtt
     5701 atggtgcgac tcgccctctt tgtaacgcca gtgagctgtt acccccatat ccgccatttt
     5761 gtccatatct tcggtgcgaa tctgtacctc caccggtaca ccgtgcgggc caatcatcga
     5821 agtgtgtaaa gattgatagc cgttcggttt tggcacggca atataatctt tcacccgttt
     5881 cggacgaggc ttatacagtg cgtgcatctg ccctaataca cggtaacaat tatctacatt
     5941 atcaaccact actcggaaag cataaatatc taaaatcgaa tgaaaatgtt gatctttctg
     6001 gcgcattttt tggtaaatgg aatagagatg tttttcacgc ccatacacac gggcttcaat
     6061 ttgcacctct tctaaccgac cacgaatatc atcggcaatg cgttgaatta gctctttacg
     6121 atgtcctctg gcactggcaa tagccatttt cagtacccga taacgttgcg ggtgcattgc
     6181 ctcgaagcct aaatcttcca actcgttttt aatatgctct attcccaagc gatgtgccaa
     6241 cgggctataa atctctaagg tttctttcgc tatacggcgg cgtttatccg gacgcaaagc
     6301 accaagcgta cgcatattat gggttcggtc ggcaagttta attaacacaa cccgaatgtc
     6361 tttggtcatt gccaaaatca ttttacggaa gtttgccact tctgcctctt ggcgggtgcg
     6421 gaattttaat ttatccagct tagaaacgcc ttgtacaata tctgctacgc tctgtccaaa
     6481 ttccgccgcc agttcttctt cggtgtaagg tgtatcttca atcacatcgt gcaacaaagc
     6541 cgcaatcacc gcttcttgat cgagcttcat ttccgcaata atcgacgcca ctgctaccgg
     6601 gtgagtaata tagggctcac cgctagagcg tgtttgtccc tcgtgagcat cacgggctat
     6661 cacataagca cgttttacac gctcaatctg ctcgggtggc aaatagcctt gaataattcg
     6721 atctaaaggt tcaaaaagat acacgttata cctcaacaac agaaataaaa atggacaacg
     6781 aatttgcaaa ttttgaggaa aatcctaccg cttgcagact caattgtcca ttctctattt
     6841 acgaaaaata aaattacgct tcgttgctca tttcagttaa aaatgaaatc gcctcttgct
     6901 ccgccgcttc ttgcttaata gagtcgtgat tttccagctg atccataatg tcagttgtaa
     6961 ttaaaccttc ttcaatttca cgtaatgcaa tcacggtcgg cttatcgttt tcttcaggca
     7021 caagtggctc acgggtgtga agttgtaatt gtcttgcacg acgtgcagca attaagatta
     7081 aatcaaaacg gttgccaatt ttctccgctg cttcttgtac ggttacacga gccataaatt
     7141 tactccaaag atttattcgg ttttattcat aaaaagattg cgaattatac agaaattccg
     7201 ccaattccgc aatgaaagcg ggctaaattc acaaaattta tttggtattc agtaaaatcg
     7261 ccaataattc agcctcaacc tctttgccga attttcccat tctaaaatcg gcttttagtt
     7321 gctcgaattg ctcagggttc gctgccattt tatcttttaa aaattgcgga aattcagata
     7381 acactaattc tgttttgcca ttacgaatat caaaaatagt tggaattttt tgcgcagaaa
     7441 tgaccgcttg ttgctgttca tcgctgtcaa aatgtttgcg ttcctgatta tctttgcgag
     7501 ccacatattt caccttaatg ttaggattga gctttgccat tcgctggaaa taagccacaa
     7561 tttccacaca atcggggcaa tatggctctg ccattaccag ccagtcccct tgcacgccaa
     7621 cagcttgcag tttttcttcc gtatctgtgg aaagcttgac ctccacatac acttctaatt
     7681 gattttcacg atcttcatcg ctgttaaatt ctaaatattc gtaaaaatgt gccataattt
     7741 tgtcttttcc tatctcatct taaatggatt tacgccataa aatttcacaa tcaactttaa
     7801 cgtcatctat tttctgttgc gctaactttt gctgaattaa ttcaaaggta cgttcggcaa
     7861 tttgttggtg gtcttgctta attgaatgaa tatgaaaagg tagtgcattc agtaaatggt
     7921 gatcatcaaa ggttgctaaa tgcagttctt gagtcaataa tttatccatt tgttggtgtt
     7981 cagtcagata gcgcattact ccctcaagta gggtaaaaga ggctgtgaat accgcttctg
     8041 ggagcctgcc aagtttttca actatctgag cgaacatttg atagcccgat tccggctgat
     8101 agtcttgatg cagaacccaa ttttcatcaa gtgccaaatg tgactgctcc aaaccttcac
     8161 ggaagccttt taaacgattt tgacttggcg agagtgaaag ttgtccacct aagtaaaaaa
     8221 actcttttaa gtgataagcc cgtacaatat tttccaccaa catactgata ctttgtgtat
     8281 cattactggt gatagaaggg atattcaacc cagaaatata gcggtctagc tgtaacattg
     8341 gggtatattg tgccgcattt tgataataag tcggctcatt atgtgtcgga gcagtaatca
     8401 gtaaatctac ctgtctgtct aataaacgct ctatcgcttt cttctcacct tcaaaggtat
     8461 catccgagca agcgatcact agctgtaacc cgttttcacg gcataatttc tctaaggttc
     8521 ttgaggtcag agcaaagcca taattcgtta aatcgggaat gactaaaccg atcacattcg
     8581 tgcgttgagc ctgtaatgat tttgcatata cattagcacg ataaccgtgt tttttagcga
     8641 tctctttcac tttttggcag gtttcggatt tgattctaaa atcattgccc cgcccattta
     8701 aaatcatact gactgttgtt ttagatacgc cgctgagttc cgcaatatca ctgagcgtta
     8761 ggcgtttttt agtcacactt atctcctttt agcataaaaa tgggcattac tgcccattgc
     8821 tttattctac gctaattggc aatacttttg ctactttcac ttgaatcgga cgagagcttt
     8881 ttacgacatt ttcacgctta gcgataaaaa agcgtgaagt cagcactttt tccccgccgt
     8941 ttacgaaaat ttccaccaca gaacggtcaa agaaaatctc gattgtctct aattgttcaa
     9001 ttttgcaatg acgaaccgag ccaaattttt ccatcaattc tgtttgttcg gtttggctac
     9061 gatctaagct caacacccca ttttcaaaac gtaagctgag cttttgcccc tgttcattgc
     9121 tgaaaaaatc caacacaagc ggttgatttt ccacagaaat ttgcaagtag gcagtatcca
     9181 aattctcgat tgcaaaattt tctgacattt cgaccgcttg taattcgcct acattcacga
     9241 ttgggttttg tatcactttg ccgttttgca gggaaagttt acgaggcaat gtcagtattg
     9301 aatgccactg atatttatca gttgggtaag tgagatcagg caagccgacc cagccaaaca
     9361 aaatgcgatc ggaattttcc accgtttgcg gtgcgtaaaa gtcaaagccg tagtccagct
     9421 cttcaatatg ctctgcccgt aatctgttgc cgtttagctc gccaagtgcg taagtggcgt
     9481 ggtagttatt ttggaattga tggctttcac gcagtttgcc ttgtggcgac cacacaaaca
     9541 gatctttacc accaagtgag aacagatccg gacactccca cataaacaca ttgctgttat
     9601 caaaattgtg gacttccagt tctgcgatta aacggggtgt ggcatttaaa tcgtccattt
     9661 cataaattaa cgccgtgccg gtcaagtttt cacgctgagc ccccaacaca aaacgaattt
     9721 tgccctctgc ggtaaggtaa ggtttcgggt cacgcacgtg ttcggtgtag cctgccggtg
     9781 cttggtcgat aatgcaacgc ttttccaata atttgcccga tttatcaaaa atcgcgatat
     9841 tttggtgagg aatgcgggca ttatctgatg ggcggcgtgt attgccggtg taaaaagcga
     9901 caatcttatc ttgccataaa atcgccccac ctgaataaca gccgtgggat tcaaacagct
     9961 catcgggaat cagcaaatcc cctttactga acgtttgaaa atcggtagtg atgaaatgct
    10021 gccaatgttt cattccatgt aatgccccat aaggaaacca ttgggcgaaa atatggtatt
    10081 tttcaccatc gaaaatcaag ccgtttgggt cattaaataa gcctgtttcg ggagcgagat
    10141 gataggttgg gtaaaaatcg ggatcagata aaacggtttg gcgaagagcg tttaattcgc
    10201 cctcgctttg tgcgtgcagg cttttgtatt tgccgttgtt gaaaatgtgc atagagtgtt
    10261 cctgtcattt cagaaattac gatggtgcaa gcgtccacgc ttgtaccctt ttgtaaaaaa
    10321 tgaggcacaa gcgtggacac ttgcgccatc attacattat tccgccaaaa atgcgtctaa
    10381 ctccgcttta ttcggtaatg ccgacatcgc cccttttgct gtggtggcta acgcaccgga
    10441 agcatttgct ttgcggatca cttcaactaa cacagtttca tcgtgccaat tcggcgtttg
    10501 agcaagaccg gctaataatc cgctgacaaa ggcatctccg gcaccggtgg tatccacagg
    10561 tttcaaggct ttgccggcaa cgacttggct ttttccgttg aaatggtaga ttgcaccgtc
    10621 tttgccaagg gtgataataa tcaatttctc cggatactga gcggtaatag cttgtgtggc
    10681 ttgttccaaa gtttctgtct cggttaataa ggtcagctct tcttcggaaa atttgagaat
    10741 atccgccatc gccaccacag aatttaccac tgttttcatt tcatcaaggc tcttccagag
    10801 cgattcacgc aagtttggat caaacgaaac atagccaccc gcctttttca ctcggcgaat
    10861 cccttcaatg gtagtggaac gggatgggtc gttaatcaag gcaatagaac agcagtgtaa
    10921 gaaatcacct tggttaaatt caggtaaatc gccgatttct aaaaattggt cggcactcgg
    10981 atttaccata aaagtaaaac tacgctcacc gttatctaag ccaacaatca cggtggaagt
    11041 gcggtgctga gcgtctaaaa tcatctgttt ggtgcaaaca ttttctgcct gtaacacttc
    11101 tcgcataaat ttacccagag ggtcgttacc cacacgcccg ataaatcctg cctcaacccc
    11161 taaacgagaa acgcctactg caacattcgc cggagcacca ccggcacatt ttaaatagtg
    11221 atgttcgccg tcgggaatta aatccaccac cgcatcgcct gttacccaaa tcttactcat
    11281 ctcatacacc tttaattagt cacaaaacac caagctaaaa cgatttagca tttattcgat
    11341 tatatactaa aagaccgctt gtacatacct acaagcggtc attttttctt taatttttac
    11401 agattaacca cgtatttacc tacacctaaa tcatcttctt tacgagtgcg ggcaatcacg
    11461 ctttctgccg attccaccac acgtaatccc atttggtctt cggtacgaat cacttcgcca
    11521 cggagtgagt tggtataaac atcggtgatt ttcacatcga caaatttgcc aatcatatcc
    11581 ggtgtgccaa caaagtttac tacacggtta gtttcggttc tgccggttaa ttccatcaag
    11641 tctttcttag acgggccttc aaccaaaatg cgttgttctg taccgagcat tgcacggcta
    11701 aattgcattg cttggtggtt gatgcgttgt tgtaaacggg ataatcgctc ttttttctca
    11761 tcatcggaga cattatcagg cagatctgcc gctggtgtac ccggtcgagc cgagtagata
    11821 aagctaaagc tcatatcaaa gttcacttgc tcgatcactt tcattgtttg ctcgaaatcc
    11881 tctgcggttt cgcccgggaa gcctacaata aagtccgaac taatttgaat ctcaggtcga
    11941 acagcacgca atttacggat aatcgctttg tattccaacg ctgtgtggtt acgcttcatc
    12001 attgttaata cacggtctga accactttga atcggtaaat gtaagaaact gaccaattca
    12061 ggtgtatcac gataaacttc gataatatca tcggtaaatt caatcgggtg gctggtggtg
    12121 taacgtaagc ggtcaatacc gtcaatcgct gccactaagc gtaataattc ggcaaaggta
    12181 caaatgccgc catcaaaagt ttcaccacgg taagcattta cgttttgacc gagtaaatta
    12241 acctcacgca caccttgtgc cgcaagttgg gcgatttcaa ataatacgtc atccaccggg
    12301 cgggaaacct cttcgccacg agtgtaaggc acaacgcaga acgaacaata tttattgcag
    12361 ccttccataa ttgaaacgaa tgctgtaggg ccttcggctt ttggctccgg caagcggtca
    12421 aatttttcaa tttccgggaa agaaatatcg acaatcgagc ttttaccgcc acggatttgg
    12481 ttgatcattt caggcaaacg gtgcaaagtt tgtggcccga aaacaatatc tacataaggg
    12541 gcacgatcac ggatatgctc cccttcttgt gaagccacac aaccgccaac accgataatc
    12601 aggttcggtt tatctttttt ccagtttttc caacgcccga gttgcgagaa tactttttct
    12661 tgtgcttttt cacggataga acaagtgttt agcagcaaca cgtctgcatc ttcagggttt
    12721 tcagtcagtt caaaaccgtg agtagagttt aggagatccg ccatttttga tgagtcgtac
    12781 tcattcattt ggcagcccca agtggtaata tgtaatttag ccataacgtt taaaattaat
    12841 aaataattca ataacttaga aaagaaaagc gtaaagcaat ttattatttt tgacttaaaa
    12901 taaacccttt acgcaaaatt tgtg
//
LOCUS       DANLZC010000068        12912 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.068, whole genome shotgun sequence.
ACCESSION   DANLZC010000068 DANLZC010000000
VERSION     DANLZC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12912
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.068"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(116..1108)
                     /gene="mglB"
                     /locus_tag="PXE44_001480"
     CDS             complement(116..1108)
                     /gene="mglB"
                     /locus_tag="PXE44_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL5588988.1"
                     /translation="MKRTILSAFALSVICGVNLSYAQSESRVGVTIYKYDDHFMTQMR
                     KDMQNEAKGFAGLKWFMNDAQNSQVKQLAQIETLLQHKMRVLAVNIVNANESKTIVEK
                     AKAYNVPVVFFNRTPSKQALESYEKAYFVSADPKEAGRIQGELIAKVWKSNPDFDLNK
                     DGKLQFVLLKGEPSDVSAERSQAVVESLNQQGVQAEEIYSDTARWRRTLARNKMNEWL
                     VENRAAEIEVVISNNDEMAIGALDALNAHEKRLPIFGIDALPETLALMKKGEIAGTVL
                     NDSSAQAKAVVKLVSNLAQGKPALKDTQWESHSSKTIYLPHIGIDKENLGQFLP"
     gene            complement(1193..1606)
                     /locus_tag="PXE44_001481"
     CDS             complement(1193..1606)
                     /locus_tag="PXE44_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HDL5588989.1"
                     /translation="MTAWERVFHALLFECFAIIFTVILTSWLTSHRMVDLTAVIVMIS
                     VIAMVWNVIFNWGFDKVFTGERVQRGLGIRILHSFLFEGGLLVFTIPLVMYMLDIGWW
                     QAFVMDIGMTLFVLVYSVVFNWVYDHLRLRWVKSG"
     gene            1726..2013
                     /locus_tag="PXE44_001482"
     CDS             1726..2013
                     /locus_tag="PXE44_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5588990.1"
                     /translation="MKNDKPIECEGCNTFDVGSILDNSETVATIQRTYESENEAKAAL
                     EKFTQKARDVESEPCEISSEITPIESGFELVAHFKFSCQAEVVIFQLGTRF"
     gene            complement(2059..2949)
                     /locus_tag="PXE44_001483"
     CDS             complement(2059..2949)
                     /locus_tag="PXE44_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL5588991.1"
                     /translation="MPINQSINQSINQSINQSINQSTNQPIVIALAADLGYQTQVETL
                     IKSLCYHHSHLKIYLFQKTFPNEWFDIWCQKLALLSSEIIPVKVCVDFSKYKSLEHIN
                     ETGFYRYLIPYLDEERVLYLDSDIVVDDNLSEMYWLNFNGIPLYAVEDYVHNHIPAYL
                     DLDLSPYFNSGVLLINNILWKKYSVCDELVTINEKIVNLTYGDQDALNIYFKGGWGAL
                     PIEYNYQVDAILELVLRREQEELARKNGYLDVIPKIIHYTSKFKPWKKELHTMQISTR
                     KKYWFYYHLEWNDILEQHQK"
     gene            3228..4934
                     /locus_tag="PXE44_001484"
     CDS             3228..4934
                     /locus_tag="PXE44_001484"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDL5588992.1"
                     /translation="MNTSSFNRTWAKVILSALMRYGVKHFCIAPGSRSTPLTLAAVQL
                     QQTQYAECHTHFDERGLGFYALGIAKATDSPVAIIVTSGTAVANLYPAVIEASLSHQK
                     LIILSADRPTELIGCGVNQAIHQQGIFANYPVASVNLPKANEAYHASWLVSTIEQACT
                     TQAQQGGVLHINVPFEEPLYEADINEILNTPWLRPIQGWLNNAQTKWIDQQAIQSEVS
                     MHENWDYWRTKRGVIVVGKLPPEQGTGLKTWAETMGWCLLSDVQSCIEASLPYADIWL
                     SNETVKQRLLQADIVIQFGTQIVSKRVNEFLKAFQGEYWVVEQSHDYHNPNAHHQTRF
                     IAKAHHFLRVHPPLRQKPWLLEPLALSQFCSTFIEQQVGGSLSEASLAHNIQDVLAIN
                     GNLFIGNSLFVRLVDALCKLPEGYPVYTNRGASGIDGLIATMAGIAKGSDRPTVGVVG
                     DISALHDLNSIALLTKINQPTILFVVNNSGGALFDMLPVDSKAKDQYYRLAHNFEFAQ
                     VATMFGLEYIRPFTWADLKTKLKHAYARKGVTIVEIKVNEHDGSNMYKSLLKQISQAE
                     IA"
     gene            4983..5822
                     /gene="menH"
                     /locus_tag="PXE44_001485"
     CDS             4983..5822
                     /gene="menH"
                     /locus_tag="PXE44_001485"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDL5588993.1"
                     /translation="MLSYQWHAQQGTPVVFLHGLLGSQQDWQAVLQHLQNFPQIRPLT
                     IDLPFHGNSKHITCADFANLRQQLHATLESLIGSRPFYLVGYSLGGRAALDYVLNMEN
                     PNLQGVVLEGANIGLSTENDRQIRRQNDRHWANRFRQEPIEQVLEDWYKQPVFADLSD
                     TERECYIDSRKHNQGEQIAQMLEATSLAKQPDYTEKLKQTDKNIVFFIGEKDQKFRKM
                     ASSHQLPTQLIANAGHNAHRANAKNFVEKLGLLILHSDSDLRSQSVKHNGAALLHKSP
                     SGA"
     gene            complement(5871..6548)
                     /locus_tag="PXE44_001486"
                     /pseudo
     CDS             complement(5871..6548)
                     /locus_tag="PXE44_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6634..7296
                     /locus_tag="PXE44_001487"
     CDS             6634..7296
                     /locus_tag="PXE44_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HDL5588994.1"
                     /translation="MRKTHSFQQVRKIKPTWMSVSGHIPFRNGVSIPYESTLERDFLM
                     YFTYLPSVDEVVSQPTTLPFVKNGITYTYTPDFFLSFTDGRKPMLIEVKPKAKWQKHW
                     KEWKEKWKAAIRFCQENGYVFHVYDEDRIRHLALFNLNYVQRYKRIQHEQEDIDAILA
                     QVKLMGNTTIDYLLSRFFAGSLYRMKGLQIIYHLLATKQLHCNWFLPLNEFTEVWGNN
                     DE"
     gene            7286..9214
                     /locus_tag="PXE44_001488"
     CDS             7286..9214
                     /locus_tag="PXE44_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDL5588995.1"
                     /translation="MMSNSGFTEKRYHHRLDRGRIILQKGNIYLNREDGEQYELVDYM
                     DEPSQLLVRNLNTRTTKVVSIHQLENFKMNERTDLSVDVTAISNEYWEKAQQKYEAIK
                     PLLGMDQHRPYAVKARAEDVGVNPRTLYRWLQAYNSIGSIAGLVDQKRGWQQGNSRLT
                     PEQDKLIVQVINEFYLHKQRPTTEQTIREIRRRCKIEKVESPSKETIRMRILHISEEE
                     RLRKRGQREKARNKFKPKPNSFPDADYPLSVVQIDHTPVDLIIVDSKYRKPIGRPFLT
                     VAIDIYSRMIVGYYLSLDAPSVTSVAMCIARGILPKERLLLDLGLQGSEWNAFGYPVK
                     VHVDNGPDFQALDLSKSCSAHGIHLEFRPMGRPEYGGHIERVIGTFMKEVHSLAGTTF
                     SNIKERDSYDSEKEAIMTLDEFEKWLVHYIVNVYHKRVHSALGISPEQKWKIGIFGDE
                     NEVGCGYPQLPVDEQTLLLDFLPSITRTIQHNGVTIDGLRYYDVALNMYISDSDESGK
                     SKEFLFRRDPRNISKIWFYDPKLKRYFPIPFANQAMPEMSIWEYREVRSRIANKGDKY
                     INEQQVLDGLTEMREMVAESAQRTKKARRQAERQKMHKASKPIIETKVETKAVVPVVV
                     TSNLLALDDESLSFGEVD"
     gene            9214..10101
                     /locus_tag="PXE44_001489"
     CDS             9214..10101
                     /locus_tag="PXE44_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HDL5588996.1"
                     /translation="MQYEHVHEKFRHLVTADNQERIAFLDEPRWLGYGVAKDIMDNLV
                     SLMNKPKRPRMLNLLIVGDSNNGKTTLIRRFFDLYGQAYIDSDSNAIYPILLAEAPPS
                     ANEKELYISLLERFYVPYKPTDTIAKLRYQTIHLFREFRVKMLVIDEFHSLLVGTPRL
                     QRQVMNAIKMLCNELQIPIVGVGTRDAIRVLHTDRQHASRFDVAELPIWKLDKEFQKL
                     LFQFQGILPLRESSNLQSPELATKIHTISGGNLGNVHRLLTACAVQAITSGTEKITLD
                     IIEKNSWVQPTNGYRKIIG"
     gene            10059..11165
                     /locus_tag="PXE44_001490"
     CDS             10059..11165
                     /locus_tag="PXE44_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDL5588997.1"
                     /translation="MGTTNKRLSQNNRIKKGVIHTPIYSNESISSWLIRASFDCGTDP
                     LTFTGFYWEKHRLWTLDLDRGLENIEPTIYDDVQALSGKIQLSEYSLFRRLYPINADE
                     TLAKGQVRWVIPRSARNRTHHIGQQYCPLCLDESPHLRNDWRLAWNFGCLKHNMMLAN
                     RCFCCGTLYQPHLLSAEKRHLNHCHQCGGKLEQGTMEMSLAEKLTLQGINQVLEKDSG
                     ICFGYKVSGKVWFDMLRYCINLLRRASDRPNHALAKFVVNLQIDVENICSPKTGLSFE
                     QLPLGERTNLLVNAYHILGMEADSIRSAIYLCGGTQKTFMFEKYPDFWQEFMALAPAG
                     PNRTRKRTKPQNESITAINRRWERLKRKLHLNNE"
     gene            11162..12586
                     /locus_tag="PXE44_001491"
     CDS             11162..12586
                     /locus_tag="PXE44_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5588998.1"
                     /translation="MKINVIKPQEEPCSACGKISRIWRRYNGEGYCANCYAKWFPKKE
                     CSCCHQPKRIYLSSTVCWDCEKQTDCVRCGKAAGTFKIGKITRYGAVCNVCSRYFREE
                     RECFECGQLSRTMLRASESGHSELLCLKCFQKYRFATCKNCRRYRKVHDRDNQLCRKC
                     SEQRTSQCPKCKQDMPAGYGNICADCSRRTLLFNLIRMNVHLFRHKKVKRAYKKFIFW
                     YLAKCGVSVVLKKSDDHVLFFMRCDENWGNIPDYRELVTFFKPNGLRTHLTVLRWLLD
                     TNQVKVDESLKEEMAGLERIQSLFNKLSNSVPCIANYYTQLQHKYEAGKTSLKSVRLA
                     LQPAIDFISSESVSLLPTQVQLNSYLVAKTGQIAAITGFINHLNREYHCELEIDRSLI
                     QKMKEKQLKQQAKQRLAILYKQEKLSIKEQNELLSVVLLNLHNIWIKSPKKLSISEID
                     GIAYYVDNEKKYFIPKDIHIRLME"
     gene            12701..12907
                     /locus_tag="PXE44_001492"
     CDS             12701..12907
                     /locus_tag="PXE44_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA delta(2)-isopentenylpyrophosphate
                     transferase"
                     /protein_id="HDL5588999.1"
                     /translation="MTLKAQFREWLKKEQEHDEMIGFAHIPCILSKVDSKLAIVSGNA
                     YEKKYLRYWIKCFRFDRNISKYIK"
BASE COUNT         4047 a         2639 c         2597 g         3629 t
ORIGIN      
        1 ttcgctctat tcaaatctgt tggaagaaaa gagaaaggat ttgcaagatt ttactgaaaa
       61 atgaccgctt gttatcatca ataaggggcg tatttataac gccccgctcc tccatttaag
      121 gtaaaaattg ccctaaattt tccttatcaa taccaatatg tggaagataa atcgttttag
      181 aggaatggct ttcccactgc gtatctttca gcgcaggctt accttgtgcc aaattagata
      241 ccaatttcac caccgctttc gcttgtgcag agctatcatt taataccgtg ccggctattt
      301 cccctttttt cattaatgct aacgtctcag gcaaggcatc aataccaaag atcggcaatc
      361 ttttctcgtg tgcatttaac gcatctaaag caccaattgc catttcatca ttgtttgaaa
      421 tcacaacttc aatctcagct gcacggtttt ccaccagcca ctcattcatt ttattgcgag
      481 ccaatgttct acgccatctc gccgtgtcac tgtaaatttc ttctgcctga acaccttgtt
      541 gattaagcga ttcaaccaca gcttgagaac gctcagcgct cacatcactc ggctcgccct
      601 taagcaatac aaattgcaat ttcccgtctt tatttagatc gaaatcgggg tttgatttcc
      661 acactttcgc aattaattcc ccttggattc taccggcctc tttaggatca gcgctcacaa
      721 agtacgcctt ttcgtaactt tctaacgctt gtttactcgg tgtgcggtta aaaaagacaa
      781 ccggcacatt ataagccttc gctttttcca caatagtttt cgactcattt gcattcacaa
      841 tattcaccgc caacacacgc attttatgct gaagtaaggt ttcaatttgc gctaattgct
      901 tcacttggga attttgtgca tcattcataa accacttcaa cccagcgaag cctttcgcct
      961 cgttttgcat atccttacgc atttgagtca taaaatggtc atcatatttg taaatcgtta
     1021 cccccactct gctttcagat tgtgcgtaac ttagattcac accgcaaata acagaaagtg
     1081 caaaagcact caagattgta cgcttcatag aatgtccctc ttgctatgcc aaatatgaac
     1141 caattcaagc atagcgaatt ttgtgtaagg aaaccgtgat ctagttcaca atttagccag
     1201 atttaaccca cctcaggcgt aaatgatcgt acacccaatt aaaaactacc gaataaacta
     1261 atacaaataa ggtcattcca atatccatca caaacgcctg ccaccagccg atatcgagca
     1321 tatacattac caatgggata gtaaaaacca gcaaaccgcc ctcaaaaagg aatgaatgca
     1381 aaatacgaat gcctaaacca cgttgcactc gctcaccggt aaacacctta tcaaaacccc
     1441 agttaaaaat aacattccac accattgcaa tcaccgaaat catcacaatc actgcggtta
     1501 aatcaaccat acgatgagaa gtcagccaag aagttaagat aaccgtaaaa ataatagcga
     1561 aacattcaaa taacagcgcg tgaaacacac gctcccaagc ggtcattttt cctccaaact
     1621 ttgcaaaata ccaactaaat caaataatcg ttcactgatg attgcaggtt acgcaattta
     1681 gttttaaagt acctacatta ttcatcaatt tagaaggatt agccaatgaa aaacgataaa
     1741 ccgattgaat gcgaaggctg taacacattt gatgtaggtt ctattttaga taacagcgaa
     1801 acagtcgcta ctattcagcg cacttatgaa agcgaaaatg aagctaaagc ggcacttgaa
     1861 aaattcactc aaaaagcccg tgatgtcgaa tctgaaccct gtgaaatcag cagtgaaatc
     1921 accccgattg aaagcggttt tgaactggtt gctcacttca aatttagctg ccaagcagaa
     1981 gtggttattt ttcaattagg aacacgtttc taatacaaaa agctgattca aacacttgaa
     2041 tcagcttttg tatgatagct atttttgatg ttgttccaaa atatcgttcc actctaaatg
     2101 atagtaaaac caatattttt tacgcgtaga tatttgcatt gtatgcagtt cttttttcca
     2161 aggtttgaat ttactcgtat agtggataat tttcggaatc acatctaaat agccattttt
     2221 tctcgcaagt tcctcttgtt ctcttcttaa tactagttca agaattgcat caacttgata
     2281 attatactct atcggcaatg ctccccaacc gcctttaaag taaatattga gagcatcttg
     2341 atcgccataa gttaagttta caattttttc attgatagta accaactcat cacatactga
     2401 atacttcttc caaagtatat tattaattaa tagtacccca gaattaaaat aagggctgag
     2461 gtctaaatca agataagccg gtatatgatt atgaacataa tcctctactg catataaagg
     2521 tataccatta aaattaagcc agtacatttc tgataaatta tcatcaacca caatatctga
     2581 atctaaatat agtacacgtt cttcgtccag atatggaata aggtaacgat aaaaacccgt
     2641 ttcattaata tgctctaaac tcttatattt tgaaaaatct acacagactt tcacaggaat
     2701 aatttcactc gataataagg ctaatttttg gcaccagata tcaaaccatt cattaggaaa
     2761 agttttctga aatagataaa tcttaagatg agaatgatga taacaaagcg atttaattaa
     2821 ggtttcaact tgagtttgat agcctaaatc agctgctaat gcaataacta ttggttggtt
     2881 ggttgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     2941 gattggcata ctattctcca aaatacttat aagtctataa aaaatttaca cttgttctaa
     3001 tataatgaat aaaattataa actaaaacac aaattcgaaa tacgtttctt cgtaaatgct
     3061 cttaaaaaag agagagttta ctaagtaaag tgttgctcaa tagatagaaa ttttcacgaa
     3121 gttttacaaa aacattccaa ttttctccta aaaaacttcc cacaataatt tcactttttg
     3181 ctacaatcct aaccatttat cgaatttatc aatcagtaga gaagaatatg aataccagtt
     3241 cttttaatcg cacttgggca aaagtgattt taagtgcatt aatgcgttat ggcgtaaaac
     3301 atttttgtat tgctccgggt tctcgctcaa caccgctcac attagcggca gtgcaacttc
     3361 aacaaacgca atatgcagag tgccacactc attttgatga gcgtggttta ggtttttatg
     3421 cgttagggat tgctaaagca accgacagcc cggttgctat tattgtcact tccggcacag
     3481 cagtagcgaa cctttatcct gcggtgatag aggcaagcct gagccaccaa aaactgatta
     3541 ttctttctgc cgaccgccca accgaattaa tcggctgtgg ggtaaaccaa gccattcatc
     3601 agcaaggtat ttttgccaat tacccggttg ccagtgtcaa tctaccgaaa gcgaacgagg
     3661 cttaccacgc aagctggctg gtttcgacca ttgagcaagc ctgcactacc caagcacaac
     3721 aaggcggtgt gttgcatatc aatgtgcctt ttgaagagcc gctttacgaa gcagacatta
     3781 atgaaattct aaatacacct tggttacgcc caatccaagg ctggttgaat aacgctcaaa
     3841 ccaaatggat agaccaacaa gcgattcaaa gcgaagtctc aatgcacgaa aattgggatt
     3901 actggcgaac taaacgaggg gtgattgtgg tcggcaaact gccgccggaa caaggcacag
     3961 gtttaaaaac ttgggcggaa accatgggtt ggtgcttgct aagtgatgtg caatcttgca
     4021 ttgaagccag cctgccgtat gccgatattt ggctctccaa cgagactgtc aaacagcgtt
     4081 tattgcaagc ggatatcgtg attcaattcg gcacgcaaat tgtcagcaaa cgggtgaatg
     4141 aatttttaaa agcattccaa ggggaatatt gggtggtgga acaatcgcac gattaccata
     4201 atccaaacgc tcatcatcaa acccgtttta ttgccaaagc acatcacttt ttgcgggtac
     4261 acccaccact tcgccaaaag ccatggttgc ttgagccatt ggcattatcg caattctgct
     4321 ccactttcat agagcaacaa gtgggtggca gcttaagtga agcctcactt gcacacaaca
     4381 ttcaagatgt tttagcaatt aatggcaact tatttatcgg caatagctta tttgttcgat
     4441 tggttgatgc tctgtgcaaa ctgccggaag gctaccctgt ttacaccaac cgtggtgcaa
     4501 gcggtattga tggcttaatc gcaacaatgg caggcattgc aaaaggtagc gaccgcccga
     4561 ctgtcggtgt ggtaggtgat atttccgcat tacacgatct taactcaatt gcattgctca
     4621 ccaaaattaa ccagccgacc attttatttg tggtgaataa cagcggtggt gcgttgtttg
     4681 atatgttgcc tgttgatagc aaagcgaaag atcaatatta ccgccttgct cacaactttg
     4741 aatttgctca agtcgccaca atgttcgggc ttgaatatat ccgcccgttt acttgggcag
     4801 atctaaaaac caaactcaaa cacgcctatg ctcgtaaagg tgttaccatt gtggaaatca
     4861 aagtaaatga acacgacggc agcaatatgt ataaatcatt gctaaaacaa atttcacagg
     4921 cggaaattgc ctaatcacaa ctgcccctcg ccaatcctgc gaggggtaaa aatgtatcga
     4981 ctatgctttc ctatcaatgg cacgctcaac aaggcacacc cgtggttttc cttcacggct
     5041 tgctcggctc acaacaagac tggcaagctg ttctccaaca tttgcaaaat tttccgcaaa
     5101 ttcgaccgct taccattgac cttccttttc acggaaatag taagcacatt acctgtgccg
     5161 attttgccaa cctgcgacaa caactacacg ccacccttga aagcctaatc ggctcacgac
     5221 ctttctattt agtcggctac tcactcggtg gacgagcggc actcgattat gtgttaaata
     5281 tggagaatcc gaatctgcag ggcgtggtgt tagaaggggc gaatatcggc ttaagcacag
     5341 aaaacgaccg ccagattcgc cggcaaaatg accgccattg ggcaaaccgt ttccgtcaag
     5401 aacccatcga acaagtgctt gaagattggt acaaacagcc tgtttttgcg gatttaagcg
     5461 atacggaaag agaatgttat attgatagca gaaagcacaa tcaaggagag caaattgctc
     5521 aaatgctgga agccaccagc ctcgccaaac agcctgacta caccgaaaaa ctcaagcaga
     5581 cggataagaa tattgtcttt tttatcggtg aaaaggatca gaaatttcgc aaaatggcat
     5641 caagccacca actaccaaca caactgattg ctaatgcagg gcataacgct catcgggcta
     5701 atgctaaaaa ttttgtggag aaattggggt tattgatact ccactccgac agcgacctac
     5761 ggtcgcaatc ggttaagcat aacggtgcag ctctgctgca caaaagcccc agtggggctt
     5821 aatcagccgt aggctaaata taacctagta cggctcagcc gtgctaggtg ttagggctcc
     5881 ccctgcaacc gttgcaataa gcgatccatc gcccgataac caagtgcttc cgctaaatgc
     5941 agttgttgaa tattcgcctc gccggctaaa tcagcaatag tccgagaaac tttcaaaata
     6001 cggtggtagg ctcgtaccga taaaccgagc tttgtcaatg cagtttctaa aaatagggca
     6061 tcttgtgccg ataattggca atctcgctca atttctcggg tggtgagttg agaattgatt
     6121 ttacctgctc gctcaaattg catttttctg gctaaaaaca ctcgcttgcg aacctgctcg
     6181 gtggtctctc ctcgattctc cgtcccactt tgcaatgctc cttgtggcaa aagtggcact
     6241 tctatagaga gatcgaaacg gtctaagaaa ggtccggaaa ggcggtttaa ataccgcatt
     6301 acctgttggg gcgaagtgcg gttatgcgtg ccttgataat gccctgtcgg gcttgggttc
     6361 atcgctgcaa ttaattgaaa gcgtgccgga aattgtactt tagcgttggc tcgggaaatc
     6421 acaatttcgc ctgcctccaa cggctgacgt aacgcatcaa gcactttacg ttcaaattca
     6481 ggcaattcat ctaagaacag cacaccgtta tgggcaagtg aaatttcccc cggtttgggg
     6541 atcgagccac tgtaaaaact agaaataaat gtcataaaaa ctagaaatag ctgtcagatt
     6601 atttagaaaa caagaaatag gtgtcaaatt attatgagaa aaacacatag ttttcaacag
     6661 gttagaaaaa ttaaacccac ttggatgagt gtatcggggc atattccatt tagaaacgga
     6721 gtcagtattc cctatgaatc gactttagag cgtgattttt tgatgtattt cacttatcta
     6781 ccttcagtag atgaagtcgt ctctcaaccg actaccctac ctttcgtcaa gaatgggatc
     6841 acttatacct atacaccaga tttcttcttg tcatttacag acggtcgaaa gccgatgtta
     6901 atcgaggtca aaccgaaagc aaaatggcag aagcactgga aagaatggaa agaaaaatgg
     6961 aaagctgcaa tacgtttttg ccaagaaaac ggttatgtat tccatgtgta tgatgaagat
     7021 cggattcgac atctagcgct tttcaatctc aactatgttc agcgctataa gcgaatacaa
     7081 catgaacaag aagatataga tgctattcta gcccaagtaa agctgatggg aaataccact
     7141 attgactatt tactctctcg attttttgca ggttctctat atcgcatgaa aggcctacaa
     7201 atcatctatc acttactggc aacgaaacaa ctacattgca attggtttct accacttaat
     7261 gaatttaccg aggtatgggg gaataatgat gagtaactct ggatttacag aaaagcgcta
     7321 tcatcaccga ttagatcggg gacgtattat tttgcaaaaa ggtaatattt acctaaatag
     7381 agaagatgga gaacagtatg aattggttga ttatatggat gaaccctccc aactgctcgt
     7441 acgaaatctc aacactcgta caaccaaagt cgtcagtatt catcagcttg aaaatttcaa
     7501 aatgaacgag cgaacggatc tttctgtgga tgtaaccgct attagtaatg aatattggga
     7561 gaaagcccaa caaaaatatg aggcaattaa accgttatta gggatggatc aacatcggcc
     7621 ttacgcagtt aaggctcgtg cagaagatgt tggggtgaac cctcgtacat tataccgttg
     7681 gttacaagcc tataattcca taggatctat cgcaggttta gtcgatcaaa aacgtggatg
     7741 gcaacaagga aattcccgtt taacgccaga gcaagacaag ctgatcgtgc aggtaatcaa
     7801 tgagttttac ctacataaac aacgcccaac tactgagcaa actattagag aaattcgccg
     7861 ccgatgcaaa attgaaaaag tggaaagccc aagcaaagaa accatccgta tgcgcatttt
     7921 gcatatttca gaggaagagc gattacggaa acgcgggcaa cgagaaaagg cacgcaacaa
     7981 atttaagccg aagccaaata gctttcctga tgcggattat ccattgagtg tggtacaaat
     8041 tgaccataca ccagttgacc taattattgt ggatagtaag tatcgtaaac cgattggccg
     8101 accgtttttg acggttgcga tagatatata cagcagaatg attgttggtt attacctttc
     8161 acttgatgcg ccttctgtaa cttcggtggc gatgtgtatt gcacgtggta ttttgccgaa
     8221 agaacgccta ctgcttgatt taggtttgca aggctcagaa tggaatgctt ttggctaccc
     8281 agtgaaagtg catgtggata atggcccaga ttttcaggct ttggatttat ctaaatcttg
     8341 ttctgcacac ggaatccatc ttgaatttag accgatgggg cgacctgaat atggcggaca
     8401 tattgagcgt gttatcggta catttatgaa agaagttcac agtttagcag gaacaacatt
     8461 ctccaacatc aaagagcgtg actcttacga ttcggaaaaa gaggccatta tgactctcga
     8521 tgagtttgaa aaatggttgg tacattacat tgtgaatgtt taccacaaac gtgtgcattc
     8581 tgctttaggc atttctcctg aacaaaaatg gaagattggt atttttggag atgaaaatga
     8641 agtgggatgt ggttatccgc aacttcctgt tgatgaacaa acactactac ttgatttctt
     8701 gccaagtatc actcgtacca ttcagcataa tggtgtaaca attgacggac ttcgttatta
     8761 cgatgttgcg ctaaatatgt atatcagtga ttcagatgaa agcggtaaat ctaaggaatt
     8821 tctgtttcgc cgagacccgc gaaatatcag caaaatttgg ttttatgatc cgaaattaaa
     8881 acgttacttc ccaattccat ttgcaaatca ggcaatgccc gaaatgagca tttgggaata
     8941 tagagaggtt cgtagccgaa ttgcgaataa aggtgataaa tatatcaacg agcaacaagt
     9001 tttagacggt ttaaccgaga tgcgtgaaat ggttgctgaa tcggctcaac ggacgaaaaa
     9061 agcacgccgt caggctgaac gacaaaaaat gcacaaagca agtaagccaa ttattgaaac
     9121 aaaagtggaa actaaggctg ttgtaccagt cgtagtaaca tcgaatttgc ttgctttaga
     9181 tgatgagagc ttatcttttg gtgaggtgga ctaatgcaat acgaacatgt tcatgaaaaa
     9241 ttccgccatc ttgtgacagc ggacaatcag gagaggattg cgttcttgga cgagccaaga
     9301 tggttaggtt atggtgtcgc caaagacatt atggataatt tggtttcttt aatgaataaa
     9361 ccaaaacgcc cacgaatgtt gaatttgttg attgtcggcg attccaataa tggaaaaacc
     9421 acactgatcc gccgcttctt cgacttgtac ggtcaggcgt atatcgacag tgattctaat
     9481 gcgatttacc ctattcttct tgccgaagcc ccaccaagtg cgaatgaaaa agaattgtac
     9541 atctcattat tagagcggtt ctacgtacca tataagccga cagatacgat agccaaactt
     9601 cgttatcaga ctatccattt gttccgagaa tttcgtgtca aaatgttggt tattgacgag
     9661 tttcattctc tgttggttgg tacaccacgc ttacaacgac aggtgatgaa cgcaattaag
     9721 atgctttgta acgagctaca aattccaatt gtcggtgttg gtactcgtga tgcaattcgg
     9781 gtattacata cggatcgaca acatgccagt cgttttgatg ttgcggaatt gccaatatgg
     9841 aaactagata aggaatttca aaaactccta tttcaattcc aagggatttt acctttgagg
     9901 gagagttcaa atctgcaatc tccagaactt gctacgaaga tccacaccat ttcaggtggg
     9961 aacttaggga acgtgcatcg cttactaaca gcttgtgcag ttcaagccat tacaagtggc
    10021 acggaaaaaa tcaccttaga catcattgag aaaaattcat gggtacaacc aacaaacggc
    10081 tatcgcaaaa taatcggata aaaaaaggtg ttattcatac ccctatttat tcaaatgaaa
    10141 gtattagctc gtggttaatt cgtgcatcct ttgattgtgg tacagatcca ctgactttta
    10201 cagggtttta ttgggaaaaa catcggttgt ggacattaga tttagatcgt ggcttggaaa
    10261 atattgagcc aactatctat gatgatgtac aggcattatc gggtaaaatc caactttcag
    10321 aatattcctt gtttcgcagg ctttacccta taaatgcaga tgaaacctta gctaaaggtc
    10381 aagtaagatg ggtgattcca cgttcggcaa ggaatagaac ccatcacatt ggtcaacaat
    10441 attgtccttt gtgcttggat gagtcgccac atttacgcaa tgattggcgg ttagcgtgga
    10501 attttggttg tctcaagcat aatatgatgt tggcgaacag atgtttttgt tgtggtacat
    10561 tgtatcaacc ccatttacta tcagcggaaa agaggcattt gaatcattgt catcaatgtg
    10621 gtgggaaact ggaacaaggc acaatggaaa tgagtctagc tgaaaagctg acattgcaag
    10681 gtattaatca ggtactcgaa aaagattcag gcatctgttt tggatacaag gtgtcgggta
    10741 aggtgtggtt tgatatgctc cgctactgca tcaacttatt gagacgtgca tcagacagac
    10801 caaatcatgc cttagcaaaa tttgtagtta atctacagat tgatgttgaa aatatatgta
    10861 gccccaaaac aggactttct tttgaacaac ttccgctagg tgaacgtaca aatttacttg
    10921 tgaatgccta tcatattttg ggaatggaag cagattctat tcgtagtgca atttatttgt
    10981 gtggcggaac acaaaaaacc tttatgtttg aaaaatatcc tgatttttgg caagaattta
    11041 tggcactagc acccgcaggc ccaaatagaa cacgaaaacg caccaagccc caaaatgagt
    11101 ctatcacagc aattaatcgc cgttgggagc gtttgaaacg caaactacac ttaaataacg
    11161 aatgaaaatt aatgtaataa aaccacagga agaaccttgc tccgcttgtg gcaaaatttc
    11221 tcgcatttgg cgcagatata atggcgaagg ctactgtgcc aattgctatg ctaagtggtt
    11281 tccgaaaaaa gagtgcagct gttgccatca accaaagcgt atctatttga gttcaacagt
    11341 atgttgggat tgcgaaaaac agactgactg tgtacgttgc ggtaaggctg cgggtacatt
    11401 caaaattggt aaaattaccc gttatggtgc agtttgtaat gtgtgttcac gttattttcg
    11461 tgaagaacga gaatgttttg agtgtggtca actatctaga actatgcttc gagcatcaga
    11521 gtcaggtcat agcgagctac tctgcttgaa gtgttttcaa aaatatcggt ttgcgacttg
    11581 taaaaactgt cgccgttatc gcaaagttca tgaccgtgat aatcagcttt gtcgaaaatg
    11641 tagtgagcaa cgaacttcac aatgcccgaa gtgcaaacag gatatgcctg cgggatatgg
    11701 taatatttgt gcagattgtt ctaggcgaac tctactgttc aaccttattc gaatgaatgt
    11761 gcatttattc cgccacaaaa aagtgaaaag agcatataaa aagttcattt tttggtatct
    11821 cgcaaagtgt ggcgtgagtg ttgtgctgaa aaaatcagac gaccacgtcc tcttttttat
    11881 gcgttgcgat gaaaattggg gcaacattcc agactaccgt gagttggtta cattctttaa
    11941 acccaatgga ttacggacgc atttgactgt gttgcgttgg ttgcttgata ccaatcaagt
    12001 caaagtagat gagagcttaa aagaggaaat ggcggggttg gaacgtattc aatccctatt
    12061 taacaaatta agtaactctg taccttgtat tgcaaactac tacacgcagt tacaacataa
    12121 atatgaggcg ggcaaaacat cactgaaatc tgttcgatta gctttacaac ctgcgattga
    12181 tttcatcagt tcagaatctg tctcgttact gccgactcaa gtacaactga atagctatct
    12241 tgtggcaaaa acgggacaga ttgctgctat tacaggcttt ataaaccacc tgaaccgaga
    12301 atatcattgt gaattagaaa ttgaccgctc cctcattcaa aagatgaaag aaaagcagtt
    12361 gaagcagcaa gcaaaacagc gattagcgat actctacaag caagaaaaac taagtatcaa
    12421 agagcaaaat gagctactct cagtcgtatt actgaatttg cacaacattt ggataaaatc
    12481 accgaaaaaa ttaagtattt cagagataga cggtatagcc tactatgttg ataatgagaa
    12541 aaaatacttt ataccaaaag acattcacat acgcctgatg gaataagctg tcgtcaattt
    12601 ttatttttgc gatctgtgtc gagaaaataa tatttatgaa tcgacagatg aaataatatt
    12661 gataaagtct ctttgtgtgt tcaacaaaca ggagataaat atgacattaa aggctcaatt
    12721 tcgtgaatgg cttaaaaaag agcaagaaca tgatgaaatg atagggttcg cccatatccc
    12781 ttgcatttta tctaaagtgg atagcaaact ggcgattgtt agcggaaatg cttatgagaa
    12841 gaaatatctc agatattgga tcaagtgttt taggttcgat agaaatatca gcaagtatat
    12901 taaataggtt aa
//
LOCUS       DANLZC010000069        12802 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.069, whole genome shotgun sequence.
ACCESSION   DANLZC010000069 DANLZC010000000
VERSION     DANLZC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12802
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.069"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(242..1375)
                     /locus_tag="PXE44_001493"
     CDS             complement(242..1375)
                     /locus_tag="PXE44_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis PLP-dependent protein"
                     /protein_id="HDL5589000.1"
                     /translation="MTKFNNIETTLVQIGNRTDPRTGAVAMPIILSTAYGRDGLGEST
                     GWDYTRTKNPTRAVLEQGIADLEGGDAGFAMASGMAAIQLVMSLFKAPDEWIISSDVY
                     GGSYRLFDFSHKNHGTLKPVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVAA
                     IAKIAKKHNLMLIVDNTFLTPALFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDS
                     DATKVNGQNLTERLFYFQNCAGAVLSPFDSYLAVRGMKTLALRMERHQSNATELAKFL
                     AEQPEIDSVLYSGKSGMLSFRLQKEEWVAKFLKAIKLITFAESLGGAESFITYPATQT
                     HMDIPEAERITRGITNNLLRFSVGLEHVEDIKADLLQAFKQLK"
     gene            complement(1392..2204)
                     /gene="dapB"
                     /locus_tag="PXE44_001494"
     CDS             complement(1392..2204)
                     /gene="dapB"
                     /locus_tag="PXE44_001494"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL5589001.1"
                     /translation="MTLKIGIVGAGGRMGRQLIQAVHNAQGVELGSAFERKGSSLVGS
                     DAGEVAGVGTLGVVISDDVAQNVAGFDLLIDFTRPEGTLEHLDICVAHHKQMVIGTTG
                     FDDAGKAAIKAASEKIGIVFASNYSVGVNLVFKLLEKAAKVMGDYCDIEVIEAHHRHK
                     VDAPSGTALSMGEHIAKTLGRDLKTHGVFERNGITGERKRDEIGFATIRAGDVVGEHS
                     VWFADEGERVEIAHKASSRMTFANGAVRAAKWLNGKQNGLFDMTDVLDLNNL"
     gene            complement(2255..2632)
                     /locus_tag="PXE44_001495"
     CDS             complement(2255..2632)
                     /locus_tag="PXE44_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589002.1"
                     /translation="MGIKVLFGTKISAELQYLPEKDLVKILQFKQHVEIYGLENLAGR
                     NKSSDDVPTNDPYWAEKVAKAQKYSLWHYHIGIPEYDTSQGFGNYTSEYILHYVKGEN
                     FIQIVDFTSHPPFKLPSESYLQN"
     gene            complement(2622..3095)
                     /locus_tag="PXE44_001496"
     CDS             complement(2622..3095)
                     /locus_tag="PXE44_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589003.1"
                     /translation="MMKNLPLSMADTLAVIYPAFDFAADDKPATEQQISLLNFRITMA
                     NQLLIACIETIEKNPNFWAEIDLNDFKKYLNQNKVVIFDTQQYFTDGSRTMAILDNMK
                     SNIRKIEQQLSQATAFNFDLERMKERLNDTFHEVPKDITNVNEFRNWLKGVANGH"
     gene            complement(3163..4251)
                     /gene="rlmM"
                     /locus_tag="PXE44_001497"
     CDS             complement(3163..4251)
                     /gene="rlmM"
                     /locus_tag="PXE44_001497"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDL5589004.1"
                     /translation="MFNKLALYCRAGFEKEVAGEITDKAAQQGIFGFANLKENSGYVI
                     FECYQAGEADRLAQALKFEQLIFVRQMLVVGDLLQNLSENDRISPILAQYAELNPRQS
                     SDVFVETPDTNEAKELLTFCRKFTVPLRSALKKQGWLGKSENMQGSVSLHILFTRPGA
                     CYVGYAYNHNKSPFFMGIPRLKFPAEAPSRSTLKLEEAILTFIPQAEEKKRFNDTMVG
                     VDLGACPGGWTYQLVKRGVFVYAVDHGKMAASLHETGRIEHCPEDGFKFQPPKRKQID
                     WLVCDMVEQPIRISKLIAKWLTNGWCRETIFNLKLPMKKRYQEVQLCLNFLEEELTKH
                     GFWFKMQAKHLYHDREEITVHIAVMGQK"
     gene            complement(4251..4643)
                     /locus_tag="PXE44_001498"
     CDS             complement(4251..4643)
                     /locus_tag="PXE44_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDL5589005.1"
                     /translation="MKNKFVAIAAFSGLFTVAFGAIASHALSAQLSPKALGWIETGIK
                     YQMFHTLAILALGLFQIANFGQNPPVCRAKAFNIIGSNWALGILLFSGNLYLLALNVP
                     TIHWLTPIGGLCFMLGWIALIYISVRKA"
     gene            complement(4750..6151)
                     /locus_tag="PXE44_001499"
                     /pseudo
     CDS             complement(4750..6151)
                     /locus_tag="PXE44_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
     gene            complement(6316..8673)
                     /locus_tag="PXE44_001500"
     CDS             complement(6316..8673)
                     /locus_tag="PXE44_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HDL5589006.1"
                     /translation="MNTALEKGKFVFLDKPKNTITTAAHWGALNVTVENGKVVKSEGS
                     LAKNHQNELQDVVADQLYSPVRVKYPMVRKGYLEGNKDTTLRGRDKWVRISWDKAFEL
                     VSGEIQRVREQYGSESIFGGSYGWYSSGSLHASRTLLQRYLNVTGGFTNTKGDYSTGA
                     VQVIMPYVLGNIEVYAQQTSWHTIVESTELLVIWSSNPLNTLRIAWTSAEPEGIDYLA
                     KLKASGKRIICIDPVKSETCQFLEAEWLPINTGTDVALILGIAHTLITENKHDHDFLT
                     KYTVGFEQFADYVLGKADNLPKTAEWASQICDIPEEVIKTLANDFVTHRTMLMSGWGL
                     QRLQHGEQSHWALVTLAAMLGQIGLPGGGFGLNYRYSTNGLNVNSGVRLGAINAGKPN
                     PDMPFFPVSRIADALLKPNQPYDYNGQTLHYPNIKLVYWAGGNPFAHHPDTNYLVKAW
                     QQPETMIVNECYWTPTARMADIVLPATTSYERNDLTVAGDYVAKHIIPMKQVVEPQFE
                     AKNDYDIFAELAKRAGKEQAFTEGKSEMEWLKEFYQVAFEAGEKANTPLLDFEAFWAE
                     NKVLSFEAEHQNNTFVRYQDYRTDPIKHKLATPSGKIEIFSETIAKMNYEDCKGHPTW
                     FEHHEYSGKTNEAEPLALVTPHIKYRLHSQLAYSSLRQYYEVNGREPMLIHTKDAADR
                     GITSGDIVRIFNKRGQVLAGAVVTDGIIQGTVALYEGGWYDPQDLGKTDTPLCKNGNP
                     NVLTRNEGSSKLGQGNAPNTTTVQVEKYVGDAPEVTVFKAPFFSE"
     gene            complement(8809..11415)
                     /gene="acnB"
                     /locus_tag="PXE44_001501"
     CDS             complement(8809..11415)
                     /gene="acnB"
                     /locus_tag="PXE44_001501"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDL5589007.1"
                     /translation="MENFIENYQQQVDERAALGVVPQPLSAEQTAQLVELLKNPPAEQ
                     AVFLLDLFTNRIPAGVDEAAYVKAAFLADITKGVATSPLISPAHAVKLLGTMQGGYNI
                     ETLLSALDNPELAPIAVEALSKTLLMFDNFHDVKERADQGNPFAQQVLESWANADWFT
                     SRPKLAEKLTVTVFKVTGETNTDDLSPAQDAWSRPDIPLHALAMLKNERDGIFPDENG
                     HTGPIKQLEALKEKGFPLAYVGDVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVV
                     LGGKIAPIFFNTLEDAGSLPIEVDVTNLNMGDVIDIYPYAGKICKHNSDEVLAEFELK
                     TEVLLDEVRAGGRIPLIIGRGLTHKARVALGLPESDVFIKPQSVSESNKGYTLAQKMV
                     GRACGVEGVRPGQYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAA
                     YPKPVDVITHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIG
                     ISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGTMQPGITLRDLVHAIPYYAIQQGLL
                     TVEKKGKKNIFSGRILEIEGLEDLKIEQAFELSDASAERSAAACTIKLNKEPIIEYLN
                     SNITLLKWMIAEGYDDKRTLERRIANMQAWLDNPELLEADENAEYAAVIEINLDEIKE
                     PIVCVPNDPDDARLLSQVQGDKIDEVFIGSCMTNIGHFRAAGKLLSQAQGELPTKLWI
                     APPTKMDAGLLTEEGYYSIYGKSGARVEMPGCSLCMGNQARVANNANVVSTSTRNFPN
                     RLGQGANVYLASAELAAVSALLGKLPTPQEYLDYMNNLQKDKDDIYRYMNFDQINSYV
                     KKADNVIVQVAV"
     gene            complement(11435..12682)
                     /gene="icd"
                     /locus_tag="PXE44_001502"
     CDS             complement(11435..12682)
                     /gene="icd"
                     /locus_tag="PXE44_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDL5589008.1"
                     /translation="MQTQVQIPQGETIQLNQDGSLQVPDNPIIPFIEGDGIGVDVTPA
                     MKAVLDAAVEKAYQGKRKIAWMEIYAGEKANKLYGENTWLPDETLELIRQYHVAIKGP
                     LMTPVGGGIRSLNVAMRQGLDLYNCLRPIRYYDGTPSPVKHPELVDMVIFRENSEDIY
                     AGVEWVAGSPEANKVIEFLQQEMGVKKIRFTEDCGIGIKPVSKQGTQRLVRAALQYVI
                     DNNRKSLTLVHKGNIMKFTEGAFKEWGYQVAEEFGAKLIDKGPWMSLTNPKTGEEIII
                     KDSIADAFLQEILLHPTEYDVIATLNLNGDYISDALAAQVGGIGISPGANIGFEAAIF
                     EATHGTAPKIAGQNKGNPGSLILSGEMMLRHLGWVEAADLVVKAVSKTIANKTVTFDF
                     AEMLEGVTLRSTSEFAQDIIANM"
BASE COUNT         3650 a         2967 c         2454 g         3731 t
ORIGIN      
        1 acgaagcgac agcgttagca aaaacttaaa atgatgtacg gatttaaaaa tctgttcgtt
       61 tgcttgtttt ataagcaaac taatttcgca taattattat tatgttaaat agatagttaa
      121 aataacacat ttaattaaat cttttcgtgt ttctctcctc ctttattaaa aaagaaaacg
      181 gtcagattgg taaaatctat acctaatatg accgcttgtg aatacaaaaa aattacttca
      241 attacttcaa ctgtttaaag gcttgcagta aatcagcttt aatatcttca acgtgttcta
      301 aaccaacgga aaaacgcagt aaattattcg taatgcctcg tgtaatacgt tctgcttccg
      361 gaatatccat atgggtttgt gttgcaggat aagtaataaa actttctgca ccgcctaaac
      421 tttccgcaaa ggtaatcaat ttaattgcct tgagaaattt cgctacccac tcttcttttt
      481 gtaaacggaa agataacata ccacttttgc ctgaatataa cacgctgtca atttcaggtt
      541 gctctgctaa gaatttagcc agttcggtgg cattagactg atggcgttcc attcgtaatg
      601 ccaaggtttt cataccacgc acggcaaggt aagaatcaaa tggcgacagc actgcaccgg
      661 cacagttttg gaaatagaat aaacgctcgg tcaaattctg cccgtttact ttggtagcat
      721 cagaatcttt cgcaacaatt aagccggcta acacatcatt atgccccgaa agatacttgg
      781 tcgcactgtg aatcacaata tcagctccgt gctcaatcgg acggaataac gctggggtta
      841 agaacgtatt atcaacgatc aacattaaat tgtgtttttt cgcaatttta gcaatggcag
      901 caacgtcgca ctcctccatc aatggatttg acggcgtttc cacgaaaatc gccttagtat
      961 tcggtgtaat agctgcttca atcgctgcta aatccgctgt attaacataa accggcttta
     1021 atgtgccgtg atttttgtgg ctaaaatcaa acaagcggta agaaccacca taaacatcgc
     1081 tggaaataat ccattcatca ggtgctttaa ataatgacat tactagctga atcgctgcca
     1141 ttccagatgc catcgcaaaa ccggcatcac cgccttctaa atccgcaatc ccctgctcta
     1201 atacagctcg agttgggttt ttggtacggg tgtaatccca gccggtagat tcgcctaagc
     1261 catcacgccc gtaagcagtt gataagataa tcggcattgc caccgcccct gtacgaggat
     1321 cagttctgtt accgatttgc actaatgtgg tttcaatatt attgaatttt gtcattgtta
     1381 ttatccttca attacagatt atttagatcc aatacgtctg tcatatcaaa caagccattt
     1441 tgtttgccat ttaaccattt tgcagcacgc actgcgccat tggcaaacgt cattcgacta
     1501 gaggctttat gtgcgatttc gacacgttcg ccttcatcag cgaaccatac actatgctcc
     1561 cctacaacat caccggcacg aatagtagca aagccgattt catcacgttt gcgttcgcct
     1621 gtaataccgt tacgttcaaa tacgccatga gttttcaagt cacgcccaag agttttggca
     1681 atatgttctc ccattgataa agccgtacct gatggtgcat caactttatg gcgatggtgt
     1741 gcctcaatca cttcaatatc acaataatcg cccattactt ttgctgcttt ttcgagcagt
     1801 ttaaacacca gattcacccc aacgctgtag tttgaggcaa aaacgatacc gattttttca
     1861 ctcgctgctt tgattgcagc tttacctgca tcatcaaaac cggttgtgcc aatcaccatt
     1921 tgtttatgat gagccacaca aatatccaaa tgttctagcg tgccttcagg gcgagtaaaa
     1981 tcaattagta aatcaaaacc tgctacattt tgggcgacat catcagaaat caccacacct
     2041 aatgtaccaa ccccggctac ttcaccagca tcactaccta ctaatgatga acctttacgc
     2101 tcaaaagcag accctaattc tacgccttgt gcattatgga cggcttgaat taattgacgc
     2161 cccattcgcc caccggcacc gacgattcca atttttaatg tcatatttgt tcttcctttt
     2221 tatatttgtt ttttacaagc ggttacattt ttaattaatt ttgcaaatag ctttctgatg
     2281 gcaatttaaa aggtgggtga gaagtaaaat caacaatttg aataaagttt tcacctttca
     2341 cataatgtaa aatatactca gaagtataat taccaaatcc ttgactggta tcatattctg
     2401 gaatgccgat atgataatgc cataaagaat atttttgtgc tttagccacc ttctctgccc
     2461 aataaggatc attcgttggc acatcatcag aacttttatt tcgaccagct agattttcca
     2521 atccataaat ttcaacgtgt tgtttaaact gcaggatttt gactagatct ttttcaggca
     2581 aatattgcaa ttcggctgaa atttttgtac caaataaaac tttaatgccc atttgcaaca
     2641 cctttcaacc agtttctaaa ttcattaaca ttcgttatat ctttaggcac ttcgtgaaaa
     2701 gtatcattta accgctcttt cattcgctct aagtcaaaat taaatgcggt tgcttgagag
     2761 agttgctgtt caattttgcg aatattggac ttcatattat ccaatatagc cattgtcctt
     2821 gagccatcag taaaatactg ttgggtatcg aaaatcacca ccttattttg atttaggtat
     2881 tttttgaaat cgtttaaatc aatttcagcc caaaagtttg gatttttttc gatagtttca
     2941 atacaggcaa ttaataattg atttgccatt gtaatacgaa aatttaacaa tgagatttgc
     3001 tgctctgttg caggtttatc atcagctgcg aaatcaaatg ccggataaat cactgcaagc
     3061 gtgtcagcca tagataaggg taaatttttc atcatctctc cttacggaag aagttgtcat
     3121 tatctactgg attacttaaa aaaacaactt attcatttta tgctattttt gccccatcac
     3181 cgcaatatgt accgtaattt cctcacggtc gtggtagagg tgtttagctt gcattttgaa
     3241 ccaaaacccg tgtttcgtta gctcttcttc caagaaattg aggcatagtt gcacttcttg
     3301 ataacgtttt ttcatcggca gttttaggtt gaaaatcgtt tctcgacacc aaccgttagt
     3361 cagccatttt gcaatgagtt tcgagatacg aatcggctgc tctaccatat cacaaaccag
     3421 ccaatcgatt tgtttacgct taggcggttg gaatttaaac ccatcttcag ggcaatgctc
     3481 aattcttccg gtttcgtgta aacttgctgc cattttgccg tgatcgactg catagacaaa
     3541 aacaccacgt ttgactaatt gataagtcca accacccgga caagccccaa gatcgacccc
     3601 taccatcgta tcattaaagc gttttttctc ctctgcttgc ggaataaaag ttaaaatcgc
     3661 ctcttctaat ttaagcgttg agcgactagg tgcctcagcc gggaatttta aacggggaat
     3721 ccccataaaa aacggcgatt tattatggtt gtacgcatag cccacatagc aagctcccgg
     3781 acgagtaaat aaaatatgca agctcacact accctgcata ttttccgatt tccccaacca
     3841 accttgtttt ttcaatgccg accgtaatgg cacagtaaat ttacgacaga aagttaataa
     3901 ttctttcgct tcgttggtat ccggtgtttc gacaaataca tcagaacttt ggcgaggatt
     3961 aagttcggca tattgggcta aaatcggcga aattcgatca ttttcagaca gattttgcaa
     4021 caaatcccca acaaccaaca tttggcgaac aaaaataagc tgttcaaact ttaacgcttg
     4081 ggctaatcga tctgcttccc ctgcttgata gcactcaaaa atcacatagc cactattttc
     4141 tttaaggttg gcaaagccaa aaatgccctg ttgtgcagct ttatccgtga tttcgcctgc
     4201 tacctctttt tcaaaccctg ctcggcaata taaggctaat ttattgaaca ttaggctttt
     4261 ctcacactga tataaattaa cgcaatccaa ccaagcataa agcagagacc accaataggc
     4321 gtaagccaat gaatagtcgg cacatttaac gccagtaagt acaggttgcc actaaaaagc
     4381 agaatgccta acgcccaatt gcttccgatg atgttaaagg ctttggctct acaaacgggt
     4441 ggattttgcc caaaatttgc aatttggaat aaacctaaag ctaaaatggc aagggtgtga
     4501 aacatttgat atttaatccc cgtctcaatc cagcctaagg ctttaggcga taattgagcc
     4561 gataatgcgt ggctggctat cgccccaaat gccacagtaa ataagccgct gaatgcggca
     4621 attgctacaa atttgttttt cataagttga agataggtat gatgttagat ggcttactat
     4681 atcatattat cgaaaccaac aagcggtgaa attttgcaaa aaaattacaa aaaatcaccg
     4741 tttgtatgct taatggttca agataatatc cacatcttcc gatttatcgt gaaccccaaa
     4801 tctatctact aaggttttgc tggcctcatt taatccgata atttctacct caacatcact
     4861 ttttcgatat ttcaacacaa ctttatctaa cgctgaaact gccgttacgt cccaaaagtg
     4921 agcgtgggtt aaatcaatgt ggactttttt cagttctgaa cgggtaaaat caaattgtgc
     4981 gataaaatca tcagatgagg caaaaaatac ttgcccgata acatcataac gcaattcctc
     5041 tttctctata gcacttgttt taaccgccat ataacgtcca attttattcg caaagaataa
     5101 tgccgagagc aacacgccgg aaaatacacc aagggctaag ttatgtgtat aaaccacaat
     5161 tgcaacagtg actaacatca ctacattaaa gccgattggg tgcgttttta gctcttttaa
     5221 ggaatgccaa ttaaaggtgc cgatagagac cataatcatc actgcaacta atgcagccat
     5281 cggaatcact ctcaaaatat cgctcaaaaa gaccacaaaa atcagtaata atattggcaa
     5341 tgccctgccc tttacattca cggtttttat cgctgtctgt ctctgtcatt tcatcaacaa
     5401 tcgttgccgt cattaaagat tccaataacc ctactgcggc caagctgaaa gaataaggca
     5461 aaataatcca taaggtgtct aaattaagcg gaatatcggg taataaaaac accggtaagg
     5521 tatcgggtaa ttgccccata tccccaacgg tacgaacatc aatgccttgc cataatgtga
     5581 aaagtgttaa agcaataatg gtcactaatg gtgagggaat gatttttcca atcaccggta
     5641 cataaggaaa aagataaatg atagcaagcc ctgctaccac cagtgcataa gtatgccaag
     5701 ttgtattttg taactcaggt agttgtgcca taaaaattaa aatggcaagt gcattaacaa
     5761 acccaatcac tacggctcgt gccacaaacc gcataagcga gccgagtttt aaccaaccgg
     5821 caataatttg caacacgcct gttaataagg tggccgctag catatattgt aaaccgtgat
     5881 ccctcactaa gcctgctaat accagagcca ttgcaccggt tgctgcagaa atcatcgccg
     5941 gtctgccgcc cacaaaactg ataattactg caatacaaaa tgaggcatat aaaccaattt
     6001 gtggatcaac ccctgcaata attgaaaatg ctatggcttc tggaattaac gccaaagcca
     6061 ccactaaacc agagagaaca tcacctcgta catttgaaaa ccattgttgt ttaattaccg
     6121 taggcgaaaa ttgctgaaat aaagttgtca ttattaccct taaataaaaa gaaaagccga
     6181 gaaaagccga gcaaagtcgg ctaaaaatga ggcgagagag tagcaaatta gtgaaaagtc
     6241 gtcaaataaa caagcggtct tctttcgctc tttttttgca aaaaatagta gaaaaatgac
     6301 cgcttggatt aattgctatt cactaaaaaa aggggcttta aacaccgtca cttcaggtgc
     6361 atctcccaca tatttttcca cttgaaccgt ggtagtgttc ggtgcattgc cctgcccgag
     6421 ttttgatgaa ccctcattac gggttagcac attcgggttg ccgtttttgc atagtggagt
     6481 atctgttttg cctaaatctt gcgggtcata ccaaccacct tcgtataacg ctactgtgcc
     6541 ttgaatgatg ccatcagtca ccactgctcc tgccaacacc tgccctcgct tgttaaaaat
     6601 tcgcacaata tcaccgcttg taatgcctct gtcagcggca tcttttgtgt gaattagcat
     6661 tggttctctg ccgtttacct cgtaatattg acgtaatgag gagtaagcaa gctggctgtg
     6721 taaacggtat ttaatatgcg gtgtaactaa tgccaacggc tcggcttcgt tggttttgcc
     6781 tgaatattcg tgatgttcaa accaagtcgg gtgtccttta caatcttcat aattcatctt
     6841 ggcaatggtt tcggagaaaa tctcaatttt gcctgatggc gttgccaatt tgtgtttaat
     6901 cggatcagta cgataatctt gatagcgaac gaaagtatta ttttgatgtt ctgcctcaaa
     6961 gctcaacact ttattttccg cccaaaatgc ctcgaaatcc agcaatggcg tattagcttt
     7021 ttcccccgcc tcaaaggcaa cttggtaaaa ttccttaagc cattccattt cgcttttgcc
     7081 ttcggtaaat gcttgttctt taccggctcg tttcgctaat tcggcaaaaa tatcgtaatc
     7141 attcttcgcc tcaaattgcg gttcaaccac ctgtttcatc gggataatat gttttgctac
     7201 ataatcaccg gcaacggtga gatcgtttcg ctcgtagctg gtggttgcgg gcaatacaat
     7261 atctgccatt cgagcggtcg gtgtccagta acattcattg acgatcatcg tttccggctg
     7321 ttgccacgct ttcactaaat agttggtatc cgggtggtga gcaaacggat taccccccgc
     7381 ccaatagact aatttgatgt tcgggtaatg taatgtttgc ccgttgtaat cataaggctg
     7441 attcggtttg agcaacgcat cggcaattcg agacaccggg aaaaacggca tatcggggtt
     7501 aggtttacct gcattgattg cccctaaacg aacaccggaa ttaacattta gcccattggt
     7561 agaataacga taatttaagc cgaaaccgcc gcccggcaag ccaatttgcc cgagcatagc
     7621 ggcaagagtc actaacgccc agtggctttg ctccccgtgt tgcaggcgtt gcaagcccca
     7681 gccggacatc aacattgtgc ggtgagtaac aaaatcattt gctaaggttt taatcacctc
     7741 ctccggaatg tcacaaattt ggctcgccca ctctgctgtt ttaggtaaat tatctgcttt
     7801 tcctaacaca taatcagcaa attgctcgaa accaacagta tatttagtta agaagtcgtg
     7861 atcgtgcttg ttttcagtaa taagggtgtg ggcgatccct aaaatcagtg caacatccgt
     7921 gccggtatta ataggcaacc actctgcctc taaaaattga caggtctcgc ttttcaccgg
     7981 gtcgatacaa ataatccgtt taccgcttgc ttttaatttg gcgagataat caatcccctc
     8041 cggttcggca gaggtccacg caatgcgaag tgtattcagt ggattagacg accaaattac
     8101 caataattct gtgctttcta caattgtgtg ccaactggtt tgttgagcat acacctcaat
     8161 attgcctaat acataaggca taatcacttg caccgcaccg gtggaataat cgcctttagt
     8221 attggtaaag ccgcctgtga cgtttaaata acgttgcaaa agcgtgcgag aggcgtgcag
     8281 tgaaccactg ctataccagc cgtaagaacc gccgaaaatg gattcgctgc catattgctc
     8341 tcttacccgt tggatttcgc cggaaaccag ctcaaaggct ttgtcccaag agattctcac
     8401 ccacttatct cgccctcgca aagtggtgtc tttattccct tccaagtagc ctttccgcac
     8461 catcggatat ttcacccgaa ccgggctata gagctgatcc gccaccacat cttgcaattc
     8521 gttttggtga tttttcgcta aactgccctc tgatttcacc acttttccgt tttccaccgt
     8581 tacatttaat gcaccccagt gggcggcggt ggtaatggta tttttcggtt tatccaaaaa
     8641 gacaaacttc cctttttcta atgctgtgtt catttaaatt aatgcctttt ctgttacaaa
     8701 attgagagga atttactgga ttaagccaaa agttgcaacc acaagcggtt gtttttagcc
     8761 aaaaatttgc aaaacaaaac ccacctaaaa aggtgggctt caaggaagtt aaacggcaac
     8821 ttgaacgatt acattgtccg cttttttcac ataactattg atttggtcaa agttcatata
     8881 gcggtaaata tcgtccttat ctttctgcaa gttattcata tagtctaggt actcttgcgg
     8941 tgtcggcaat ttacccaata acgcagaaac tgcggctaat tctgctgagg ctaaatacac
     9001 atttgcccct tgccctaaac ggttcgggaa gtttcgggta gaggtagaaa ccacattagc
     9061 attatttgcc actcgggctt ggttacccat acagagagaa cagcccggca tctctaccct
     9121 tgctccgctt ttgccgtaaa tgctgtaata gccctcttcg gtcagcaagc ctgcgtccat
     9181 tttagtcggc ggagcaatcc ataatttagt cggcaattcg ccctgtgctt ggcttaacag
     9241 tttacctgcc gcacggaaat gcccgatatt agtcatacaa gaaccaataa agacttcatc
     9301 aattttatcg ccctgtactt gagaaagcag gcgagcatcg tcagggtcat tcggtacgca
     9361 cacaatcggc tctttgattt catctaagtt gatctcaatc actgcggcat actccgcatt
     9421 ttcgtctgcc tctaaaagct ccggattatc caaccacgct tgcatattgg cgatacggcg
     9481 ttctagcgtg cgtttatcgt catagccttc cgcaatcatc cattttaaaa gcgtaatatt
     9541 agaattcaga tactcgataa tcggctcttt gttgagcttg atggtacagg ctgcagcaga
     9601 gcgttcagca gaagcatcag aaagctcgaa ggcttgttcg attttcagat cttccaaccc
     9661 ttcaatttct aaaattcgac cggagaaaat atttttctta ccttttttct ccaccgtcag
     9721 caagccttgc tgaatggcat aatacggaat agcgtgaacc agatcccgta aggtaatgcc
     9781 cggttgcatt gtgccggaaa aacgcaccaa gaccgattcc ggcatatcta acggcatcac
     9841 ccctgttgcc gcagcaaagg caactaagcc cgaaccggca gggaatgaaa tcccgattgg
     9901 gaaacgggtg tgggaatcgc cgcctgtacc gacagtatcc ggcaataaca tacggtttag
     9961 ccaagagtga atcacgccat cacccgggcg aagtgacaca ccgccacggt tcataataaa
    10021 atcaggcaaa gtgtggtggg taattacgtc caccggtttc gggtaagcgg cagtatggca
    10081 gaacgattgc atcacaagat ctgccgagaa gcctaaacag gctaaatctt tcagctcatc
    10141 tcttgtcatt ggtcctgtgg tatcttgtga accgacagac gtcattcgag gctcgcaata
    10201 ttgccccggt cgaacgcctt ctacgccaca agctcgccct accatttttt gagcaagggt
    10261 gtagcctttg ttactctctg aaaccgactg cggtttaata aatacatcac tttcaggcaa
    10321 gccaagtgct actctggctt tatgggtcaa accacgtccg ataatcagcg gaattcgccc
    10381 gcctgcacgc acttcatcaa gcaatacttc ggtttttagc tcaaattcgg ctaagacttc
    10441 atcggagttg tgtttgcaaa ttttgcctgc ataagggtaa atgtcgatta catcgcccat
    10501 attcaggttg gttacatcca cttcgatcgg taaagaaccg gcatcttcta atgtgttaaa
    10561 gaaaatcggg gcaattttac cgcctaacac caccccaccg gcacgtttgt ttggaatgta
    10621 aggaatatct tcccccataa accaaagcac ggagttggtt gcggatttac gagaagagcc
    10681 ggtgccgact acatcaccga cataggctaa cgggaaacct ttttctttta atgcttccag
    10741 ttgcttaatc gggccggtat gaccgttttc atcggggaaa ataccgtcac gttcattttt
    10801 cagcattgct aatgcgtgaa gtggaatatc agggcgagac caagcgtctt gagccgggga
    10861 aagatcatcg gtattggttt cgcctgtgac tttaaatacc gttaccgtta atttttcggc
    10921 aagtttcggg cgagaggtaa accaatcggc attcgcccaa gattccaata cttgttgtgc
    10981 aaacggatta ccctgatctg cccgttcttt tacatcgtgg aaattatcaa acatcaataa
    11041 tgttttagat aaggcttcta ccgcaatcgg tgcaagctca gggttatcca acgcagacaa
    11101 taaggtttca atgttataac caccttgcat tgtgccgagt aatttcactg cgtgagcagg
    11161 cgaaatgagc ggcgatgtgg caaccccttt ggtaatatcg gctaaaaagg cggctttaac
    11221 ataagcggct tcatcaacac ctgccggaat acgattggta aaaagatcca ataaaaagac
    11281 cgcttgttca gccggtgggt ttttcaataa ttcaaccagt tgagccgttt gttccgcaga
    11341 aagtggctga ggcacaacac ccagtgccgc acgttcatca acctgttgtt gataattttc
    11401 tataaaattt tccatcatca ttccctctcc aactttacat attggcaata atgtcttgtg
    11461 caaattctga ggttgagcgt agagttaccc cctccagcat ttccgcaaaa tcaaaggtga
    11521 cagttttatt ggcaatggtt ttagacaccg cttttaccac taaatcagcc gcttcaaccc
    11581 aacctaaatg gcgtaacatc atttccccac ttaaaattaa tgacccagga ttgcctttat
    11641 tttgccctgc aattttaggg gctgtgccgt gtgtggcttc aaaaatagcc gcttcaaacc
    11701 caatattcgc tcccggtgaa ataccgattc cgcctacttg tgcggctaaa gcatcagaaa
    11761 tatagtcgcc gtttaggttc agtgtagcaa tcacatcata ttcggttggg tgtaacaaga
    11821 tttcttgtaa aaaggcatcg gcaatgctgt cttttataat gatttcctca ccggttttcg
    11881 gattagtcaa gctcatccaa gggcctttat caattaattt tgccccaaat tcttccgcaa
    11941 cttggtagcc ccactcttta aatgctcctt cggtaaattt cataatgtta cctttatgca
    12001 caagcgtgag cgatttacgg ttgttatcaa ttacatattg caatgcagca cgcaccaagc
    12061 gttgtgtgcc ttgcttagaa accggcttaa ttccgatacc gcaatcttcg gtaaagcgga
    12121 tttttttcac gcccatctct tgttgtaaaa attcgatcac tttattggct tcaggcgaac
    12181 cggcaaccca ttctacaccg gcataaatat cttccgagtt ttcacggaaa atcaccatat
    12241 ccaccagctc agggtgttta accgggcttg gcgtaccgtc ataataacga attgggcgta
    12301 agcagttgta taaatctaat ccttgacgca tcgccacatt taaagaacga ataccgccac
    12361 ccaccggtgt cattaaagga cctttaatcg ccacgtgata ttggcggata agctctaagg
    12421 tttcatccgg caaccaagtg ttctcaccat ataatttatt cgccttttcg cctgcgtaaa
    12481 tttccatcca agcaattttt cgcttgcctt gataggcttt ttctaccgcc gcatctagca
    12541 ctgctttcat tgcaggagta acatcaacac cgatgccgtc accttcaata aatggaataa
    12601 tcggattatc cggaacttgt aaagaaccgt cttggttcag ttgaatagtt tcgccttgtg
    12661 gaatttggac ttgtgtttgc attgatttta cccccgtaat gacgaaaaag tagtgacgaa
    12721 aaatgaatgt tgacaatcag ccaacaatgg attaactata aacagtaaat ttctaaatgt
    12781 aaataaagca aaatgggctg ag
//
LOCUS       DANLZC010000070        12744 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.070, whole genome shotgun sequence.
ACCESSION   DANLZC010000070 DANLZC010000000
VERSION     DANLZC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12744
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.070"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            278..496
                     /locus_tag="PXE44_001503"
                     /pseudo
     CDS             278..496
                     /locus_tag="PXE44_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
     gene            complement(548..763)
                     /locus_tag="PXE44_001504"
     CDS             complement(548..763)
                     /locus_tag="PXE44_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589009.1"
                     /translation="MQTEKRGRGRPKSGLTLQELQAKSDAKRGVRLKSFKFHEDFIAQ
                     LEQLAEQHGISQTQVIVQAVEQFSKGA"
     gene            1308..2009
                     /locus_tag="PXE44_001505"
     CDS             1308..2009
                     /locus_tag="PXE44_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589010.1"
                     /translation="MSTQISTQTISFNNQALITFEQNGTHYTAMKPICENIGLTWHAQ
                     YERIKRDEILSQGILIIRIPTNGGDQQMICLPIEYLNGWLFGIDINRCKPEIRETLIK
                     YKKECYQALHDYWFKGKAERSQIAPIVEPKIPMELTKKEWLRFASMWYALYNSLEVLA
                     TLEKPLRAIGSPFGATAYTHATEYQTTLGVMKRILEPMLADFEVDPFEEAHYHKALTT
                     LRQYQPKGLGGLVRI"
     gene            2263..2439
                     /locus_tag="PXE44_001506"
     CDS             2263..2439
                     /locus_tag="PXE44_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589011.1"
                     /translation="MTEEDKEYLQTKIENEGFEYAFVSYSDFEEVQDEKFHGLRKAYL
                     KARSELAEYIDIED"
     gene            2527..2910
                     /locus_tag="PXE44_001507"
     CDS             2527..2910
                     /locus_tag="PXE44_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589012.1"
                     /translation="MHIEDKIAWWLANGETGVSSKTMAFYLGYGIRPKIEGYPHDVSD
                     FRRCFLLLETVPFLRNRIEKMAELGKVWAALAKEWHTLEALYNEEEDQIRCPKTYAKL
                     REILEANEENVVRIGNVSISIGSTT"
     gene            2960..3181
                     /locus_tag="PXE44_001508"
     CDS             2960..3181
                     /locus_tag="PXE44_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL5589013.1"
                     /translation="MNIYTDFLSREEIEFITQCKQKNLIKKQLNIMGIPFKENANGFP
                     VVRRDYAQAKQRKPIATNDSDWVSNAFKA"
     gene            3203..4243
                     /locus_tag="PXE44_001509"
     CDS             3203..4243
                     /locus_tag="PXE44_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5589014.1"
                     /translation="MARPRKYENNGLPQNLLCRRRKRANGSIVEYYFYVLADGKERSL
                     GTNKHEAILEAAKLNFENSRKSPIVLFIDVAKRYELEVVPTKKAKNTRQSNLQAIHWL
                     CKFFGNPPAPLEKIEPKHISQYLQWRKDTPAVANIEVGLFNTIWNMAREWGYTALPSP
                     SQGVKKFPTKYREVYVEDYILDKIYEFADERMADIIETAYLLGQRPIDICNIHRSHIY
                     DGILHITQQKTGKKVRFEISGRLKEILDKRLGYESDWIFTNKWGKKLKRRSLGDHFKE
                     IRERAMKAYPELADEIAKVQMRDMRAKAATDISLMTTDEQAQKQLGHTSKRMTHHYIR
                     KDKILKPTDEIT"
     gene            complement(4402..4689)
                     /gene="rplY"
                     /locus_tag="PXE44_001510"
     CDS             complement(4402..4689)
                     /gene="rplY"
                     /locus_tag="PXE44_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDL5589015.1"
                     /translation="MSFKFEAEVRSAQGKGASRRLRHNGQVPAIIYGGNAEPVSIILD
                     HDKVNNAQVHDSFYNEVLTIVVAGKEEQVKVQAIQRHPTKPKLVHLDFKRA"
     gene            complement(4841..6109)
                     /locus_tag="PXE44_001511"
     CDS             complement(4841..6109)
                     /locus_tag="PXE44_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HDL5589016.1"
                     /translation="MSDLKQQALDFHEFPIPGKISVTPTKALDTQHDLALAYSPGVAE
                     PCLAIEKDPSASYRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAG
                     IDVFDIEINETDPEKLVEIIASLEPTFGGINLEDIKAPECFYVEQKLRERMGIPVFHD
                     DQHGTAIITGAAVINGLQVVGKAISEVRLVVNGAGASAIACTNLLRSLGVKKENITMC
                     DSKGVIFQGRGDKMDETKQFYAIEDNGWRTLADAVANADVFLGCSKGGALTQEMVKTM
                     AKDLLILALANPVPEITPAEAKAVRPDAIVCTGRSDFKNQVNNVLCFPFLFRGALDVG
                     AKTINEEMKMAAAHAIAELAKEPVPFEVISTYGELSFGPEYIIPTPFDPRLIVNVSIG
                     VAKAAIQSGVATRPITDWEAYAEQLKKLVA"
     gene            complement(6266..7177)
                     /locus_tag="PXE44_001512"
     CDS             complement(6266..7177)
                     /locus_tag="PXE44_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HDL5589017.1"
                     /translation="MKWLMLCREPRLYSCCRIKAACEAKGIELDILDQNRMLLGLENG
                     EFKIYYQAGESYDNNRPEPVLLPNYDGILPRFGTGSTEMGCSVLRHFEAKGIPVLNHS
                     AAFALARDKWRTLQKLAKHNLPVPNTNFAGHLVSVKSQLNQFAFPLISKVLNGSQGNG
                     VMLFEGKNNAEAVLATFRQVNEPYLCQQFVGDAKGQDIRAFVIGNEVVAAMSRTSVTG
                     DFRANIHQGGTAQPIELTQMERALAIKATKTIGLDIAGVDFLRTEKGVVILEVNASPG
                     FEGIERVNDVDIASEMVSYFINKMKNA"
     gene            complement(7174..7893)
                     /gene="nfsA"
                     /locus_tag="PXE44_001513"
     CDS             complement(7174..7893)
                     /gene="nfsA"
                     /locus_tag="PXE44_001513"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HDL5589018.1"
                     /translation="MQSKPTLETILSHRSIRKFTEQPISEEIFQILIEAGKAASSSNH
                     LQCVTVIRVTDRQIRETLKAVSGMQYVVDCAEFLVFCIDFNKHKQLVPESQLEWAEVS
                     LIGAVDTGILAQNVFLAAESLGLGAVYIGALRNDIERVSKALNLPEYCVPLVGMCLGY
                     PAQEPAIKPRLPNSLVCYNNQYQDFDWTEFAKYNENLTAYYQARIGEPQQWQAAIHKT
                     LDKPVRPHILPFFNQKGLLKK"
     gene            complement(7905..8690)
                     /gene="mtgA"
                     /locus_tag="PXE44_001514"
     CDS             complement(7905..8690)
                     /gene="mtgA"
                     /locus_tag="PXE44_001514"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDL5589019.1"
                     /translation="MEQSMAKRNKRFLSSLLKKIGLFFIPRKIKSKLGWLWFGSSRLL
                     TFGGTFFLIPILIFSVLPVPYSAYMVQKKVENLVQGKSYPIKKEWVSLNEISWQMQMA
                     VIASEDQKFESHFGIDLQAIETALKLNAKSKKVRGGSTISQQTVKNMFLWHGQSWLRK
                     GIEVPLTLLVENVWSKDRILEVYLNIAEFGNGIFGVEAAAKHFFKKSAKQLTLQESAL
                     LAASLPNPLVFRVDKPGPSMRKRQAWIMRQVSLLDGKNYLDKL"
     gene            complement(8665..8985)
                     /gene="trpR"
                     /locus_tag="PXE44_001515"
     CDS             complement(8665..8985)
                     /gene="trpR"
                     /locus_tag="PXE44_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDL5589020.1"
                     /translation="MKTLYNQRDPNEWTQFLALLEQAVAEDKLEPFFSMFLTADERGS
                     LGLRLQIVQALLKGEDSQREIQQNLNTSAATITRGSNMLKTLDQDFLQWLNGKVNGTV
                     NGKA"
     gene            complement(9065..9706)
                     /gene="lolA"
                     /locus_tag="PXE44_001516"
     CDS             complement(9065..9706)
                     /gene="lolA"
                     /locus_tag="PXE44_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDL5589021.1"
                     /translation="MKTLFKKSVFGLALFGLANVALADAQSVAELQRRLEQVSQYSAD
                     FDQTVRSSKGKQIQQGKGKFQVKRPNLFRMDTKSPQENLIVSDGKDLWFYDPFVSQVT
                     VNTVQDAVNNTPFVLLTSGDKSHWDQYDVSQSADTFVLKPKSKKSNLKQFDVRIDQSG
                     LLKGFSTIERDGQSNLYVLRNITTGGVSSDLFKFSVPKGAELDDQRGGKKSKK"
     gene            complement(9817..12675)
                     /gene="polA"
                     /locus_tag="PXE44_001517"
     CDS             complement(9817..12675)
                     /gene="polA"
                     /locus_tag="PXE44_001517"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL5589022.1"
                     /translation="MATIAKNPLVLVDGSSYLYRAFHAFPPLTNRLGEPTGAMYGVLN
                     MLKSLISQVNPSHIAVVFDAKGKTFRDELFKQYKSHRPPMPDDLRPQIEPLHRIIKAL
                     GIPLISIEGVEADDVIGTLAVQAAKDGKDVLISTGDKDMAQLVNDHIMLINTMNNTLL
                     DREGVIEKYGIPPELIIDFLALMGDSADNIPGVKGVGEKTALALLQGIGSLEQIYANL
                     DQIATLSFRGAKNFAPKLEAEKANADLSYLLATIKTDVELDVTHDQLLTQPQDRAELA
                     QLFEHYEFKRWLNEVNQNANPVTQTSAEKVPNNYQVTQAVRNEENVANVVEIDRSKYE
                     TVDTEQKLSAWIEKLTAARLIAVDTETDSLDAMSANLVGISFALENGEACYIPLGHKQ
                     KAQPQQADMFGDEERSESPSSDELVKNQLNLTACLSKLKPILLNPEIKKIGQNIKYDL
                     TIFANYGIELQGVAFDTMIESYTQNSTGRHNMDDLAARYLGHKTIPFEELAGKGKNQL
                     TFDKIEIAKAAEYAAEDADVTMKLHQVLWEKLQQEPELVKLFDEIEMPLVGVLSRIER
                     NGVLIDPKKLLTQSAEIEKRLEEVETLVHAEAGQTFNLASTKQLQEILFEKLGLPVLK
                     KTPKGAPSTNEEVLDELAQQGHLVPKLLMEHRGLSKLKSTYTDKLPQLINKKTGRVHT
                     SYNQAVTATGRLSSSDPNLQNIPIRNEEGRRIRQAFVAREGYKIVAADYSQIELRIMA
                     HLSNDEGMINAFAEGKDIHRATAAEIFGVELGEVTSEQRRSAKAINFGLIYGMSSFGL
                     SNQLGIGRAEAQKYMDLYFQRYPAVQQFMTDIREVAVEKGYVETLFGRRLYLPDIKSG
                     NAILRKAAERVAINAPMQGTAADIIKVAMIGIDNAIRDNDEIKMIMQVHDELVFEVKA
                     DKIDHYSQLIKTEMEKAISLKVPLIAEVGVGENWDEAH"
BASE COUNT         3813 a         2827 c         2393 g         3711 t
ORIGIN      
        1 acttttgaca aaatacactc aacgccacag ctattaatga atggcgagtc ctgattgatt
       61 agccattgtt tgaaaagctc tttcatagtg ttctccttat atttttttat gagattacta
      121 cgaaagcgtt ttactgtcag aacaaaaatt tattttttcg atgcagatcg caaaaataga
      181 tattgaggct tgacaccgcc aaacttcgga ttaagataac cgcactttca agccgtccac
      241 caacggcttt ttttgtacct aaaaacggag aaacgaaatg gcaaatagcg taatgattgt
      301 acaaggcgtg gaggtcaaag taaccgctcg tgatggtgag gattacatca gcctaactga
      361 tatgtgcaaa gcttttggag acagcgacca gctcattaaa aactggcttc aaaataaaaa
      421 taccattgaa tttcttcaag tttgggaaga gctgaataat ccaaatttta atttggtgga
      481 attacaccaa attaaatgca acaactaatt aaaaacatcg aacagtgaag ggtaggttta
      541 taggggctta cgcccctttt gaaaactgct caaccgcttg aacgattacc tgtgtttgcg
      601 aaatgccgtg ttgttctgca agctgttcaa gttgagcaat aaaatcttcg tggaacttga
      661 atgacttcaa tcgcacacca cgctttgcat cgctttttgc ttgtaattct tgaagcgtta
      721 aacctgattt agggcgacca cgacctcgtt tctctgtttg cattgtcaat tccttgttga
      781 tttttaatac ggaacgttat attatttagg aactgcctag cagagatggc gttctctgct
      841 aggactttta gctttctaat ctaataagct ggcgtgctta ttaagattaa gatcactagg
      901 attaacaact taagatacat aatccagttc ctgatgtttg ccgctctctt caagggcggc
      961 ttcttcattt tcagaccctt tccgaaaaca agattatttt aatacatacc aaataaaacg
     1021 caagctattt tttagtattt attaaaataa ttttatttga caaccgccaa actttaactt
     1081 actattccgc ccaaggtgtc gaaacctcat actctaaggc ggatagttca ctaatcgcca
     1141 ctaggcgatt tttttatatc cgtaatcctg actatgtcgg gagggcgact aatacaagac
     1201 cgaaaggaaa tacgtccagc ccttacctta gagtgggttt tcgaacctcc cgacgccact
     1261 gtcgaaagtg gcttgtttta aacaaataga ctctaaggat tacgactatg tcaactcaaa
     1321 tttctactca aaccatttct ttcaacaacc aagccttaat cacattcgag caaaacggca
     1381 cacattacac cgcaatgaaa ccgatctgcg aaaatatcgg cttgacttgg cacgctcaat
     1441 acgaacggat caaacgagat gaaatccttt cgcagggtat tcttatcata agaataccta
     1501 caaacggtgg cgatcaacaa atgatctgcc tcccaattga atacctcaac ggctggctct
     1561 tcggcattga catcaatcgc tgcaaacccg aaatcagaga aacgctcatc aaatacaaaa
     1621 aagagtgcta ccaagccctg cacgattact ggttcaaggg caaagcggaa cgttcacaaa
     1681 tcgccccgat tgtcgaacct aaaatcccaa tggagctaac caaaaaagaa tggctacgtt
     1741 tcgcttcaat gtggtacgcc ctctacaaca gccttgaagt acttgctacg ttagaaaaac
     1801 cgttgcgtgc tatcggttcg ccgtttgggg caaccgccta cactcacgcc accgagtatc
     1861 aaaccacgct tggcgtaatg aaacggatac ttgaaccgat gttagccgac ttcgaggtcg
     1921 atccgtttga agaagctcac taccacaaag ccctaactac cttaaggcaa taccagccga
     1981 aaggcttagg cggcttagtc cgaatttaat ttgcaaaaaa atcacacaat ccgaccgctt
     2041 gtgaaacatc aagcggcggt ttcctgcacc caaaattcaa ggattaggcg atgaaatacg
     2101 caaaaatcat actcttttta accgcctttg cagttgctgc cgactactta gagttacaca
     2161 acgactgcga tggcaaacca tgcactatcg cacagcgata atgcttcccc gaccctgccc
     2221 gtgcagtgtt tatttttatc cactctcaat aggagaaacg aaatgacaga agaagacaaa
     2281 gaatatcttc aaactaaaat tgaaaacgaa ggttttgaat acgctttcgt cagttatagt
     2341 gactttgaag aagtccaaga tgaaaaattc cacgggcttc gcaaggctta tttaaaagct
     2401 cgaagtgagt tagcggaata tattgatatt gaagattaac cactgatgaa ctgttcattg
     2461 aacaaatgaa cacttgacaa ccaaccgctt gtagctaact gcaagcggtt aatttttgga
     2521 ggaaaaatgc acatagaaga caaaatcgct tggtggcttg ccaatggaga gacaggggta
     2581 agcagtaaaa caatggcttt ttaccttggt tacggaataa gaccaaaaat agaaggctac
     2641 ccacacgatg tatcggattt caggcgttgc tttctgttgt tggagacagt cccttttttg
     2701 cgaaatagga ttgaaaaaat ggctgaactt gggaaagttt gggctgcact tgcaaaagaa
     2761 tggcacacat tagaggctct ctacaacgaa gaggaagatc aaataagatg ccccaaaacc
     2821 tatgctaagt tgagagaaat cctagaagcc aacgaagaaa atgttgtccg catcggtaat
     2881 gtgagcattt cgataggttc aacgacttaa cgattattta accaaatccc tctaaatgag
     2941 gggtttattt ggagagaata tgaacatcta cactgacttc ctttctcgtg aagagattga
     3001 atttattact caatgcaagc agaaaaatct aatcaaaaaa caactcaaca ttatgggcat
     3061 tccatttaaa gaaaatgcaa atggattccc tgttgtccgc cgtgattatg ctcaggctaa
     3121 acagcgtaaa cctattgcaa cgaatgattc tgactgggta tcaaatgcat ttaaagcgta
     3181 aattgagaag aaggaggcac ttatggcacg ccctcgaaaa tatgaaaaca acggattgcc
     3241 acaaaaccta ctctgccgcc gcagaaaacg agcaaacgga agtatagttg aatactactt
     3301 ttacgttctt gccgacggca aagaaaggtc tctcggtaca aacaaacacg aagcaatttt
     3361 agaagcagcc aagctaaatt ttgaaaatag tagaaaatcc ccaattgttt tatttattga
     3421 tgtggcaaaa cgctatgaat tggaagttgt tccaaccaaa aaagccaaaa acactcgtca
     3481 atctaattta caggctattc attggctatg taaattcttt ggtaacccac ctgcaccgct
     3541 cgaaaaaatt gagccaaagc acatcagtca atatttacaa tggagaaaag acacgccggc
     3601 tgttgctaat attgaggtag gtttatttaa cacaatttgg aatatggcaa gagaatgggg
     3661 atacactgct cttccaagcc catcacaagg tgtaaaaaaa ttcccgacca aatatcgtga
     3721 agtttatgta gaggattata tccttgataa aatctatgaa tttgcagatg aaagaatggc
     3781 ggatattatc gaaacagcct atttgctggg acaaagaccg attgatattt gtaatataca
     3841 ccgctcacac atctatgacg gtatattgca tattactcaa cagaagacag ggaaaaaagt
     3901 gagatttgaa attagtggtc ggctaaaaga aattttagat aaaaggttgg gatacgaatc
     3961 agactggatt tttaccaata aatgggggaa aaaattaaaa cggcgaagtt taggcgatca
     4021 ttttaaagaa ataagggaga gagcaatgaa agcatatcct gaacttgctg atgaaatcgc
     4081 taaagtacaa atgcgtgata tgcgtgccaa agcagccacc gatatttccc taatgacaac
     4141 cgatgaacaa gctcaaaaac aacttgggca tacatcaaaa agaatgacac atcattacat
     4201 cagaaaagac aaaatcctca aacctaccga tgaaatcacc taaaaataaa gcccttttgc
     4261 caaatacaaa agagccattt actcacctaa aatccgttcc gaaactattt tgtaaataat
     4321 tgattttatt gacctaaaaa actataaaat cgcaaaaagt ttcggaacag aaaacagcgt
     4381 taaacccttg taaatcaagg attaagcacg tttgaagtct aagtgaacta attttggttt
     4441 agttgggtgg cgttgaattg cttgtacttt tacttgctct tctttacctg caacaacgat
     4501 agttaatact tcgttataga atgaatcgtg aacttgtgcg ttgttcactt tatcgtgatc
     4561 taaaatgatt gatacaggct ctgcattacc accgtagatg attgcaggaa cttgaccatt
     4621 gtgacgcagg cggcggctcg cacccttacc ttgcgcagaa cgaacttcag cttcaaattt
     4681 gaatgacata gttttattct cttaaataaa atggggaaat ccccgtgaat agaaaaaaca
     4741 gcaggcgacc cagctgtttc cgaaaaaata tcggcatatc cgtctcgata tgccaactac
     4801 gtattttact gaagttgcag aaaaatgcaa tgtaaaaata ttaagcaacc agttttttca
     4861 actgctctgc ataagcttcc caatctgtaa ttggtcgagt tgctacacct gattgaattg
     4921 ctgcttttgc tacaccaatc gagacattca cgatcaaacg tggatcaaat ggcgttggaa
     4981 taatatattc cgggccaaac gataattcgc cataagttga aataacttca aacggcacag
     5041 gttctttagc caactctgca atcgcatggg ctgctgccat tttcatttct tcattaatgg
     5101 tttttgcccc aacatctaat gctccacgga ataagaacgg gaaacaaagc acattattca
     5161 cttggttttt aaagtctgaa cgaccggtac atacaatcgc atctggacgg acagccttcg
     5221 cttcagcagg tgtaatttcc ggcacagggt tcgctaaagc aagaattaat agatcttttg
     5281 ccattgtttt aaccatttct tgcgttaaag ctccgccttt tgaacaacct aagaaaacat
     5341 ctgcattcgc aacggcatct gccaatgttc tccaaccatt atcttctatt gcatagaatt
     5401 gtttggtttc atccatctta tcaccacgac cttggaaaat aacgccctta gaatcgcaca
     5461 ttgtgatatt ttcttttttc acacctaatg aacggagtaa gttagtacac gcaatcgctg
     5521 atgctccagc accgtttacc actaaacgta cttctgaaat tgctttacct accacttgta
     5581 atccgttaat caccgccgca ccggtaataa ttgccgtacc gtgttgatca tcgtggaaca
     5641 cagggattcc catacgttca cgtaattttt gctctacata aaaacattca ggtgctttaa
     5701 tatcttccag attaatacca ccaaaagtcg gttctaacga tgcaataatc tccactaatt
     5761 tctccggatc agtttcatta atttcaatat caaacacatc aattccggca aatttcttaa
     5821 atagaacacc tttgccttcc atcacaggct tacttgctaa agcaccaata ttgcctaagc
     5881 ctaacactgc tgtaccatta gaaattactg caactaagtt acctcgtgcc gtataacgat
     5941 aagaagctga aggatctttt tcaatcgcta agcaaggctc agcaacccca ggagagtacg
     6001 ctaaagctaa atcgtgctga gtatctaatg ctttagtagg agtaacggaa atttttccgg
     6061 ggattgggaa ctcgtgaaaa tcaagcgcct gttgttttaa atcgctcatt ttagaaacct
     6121 tataatctat aaaaataaaa gtaatcaata atagccaaag agtagccatt ttaccctaaa
     6181 ttttttcgca tttttatggc tttgctctca ttttttatga tatttacaaa aaataataca
     6241 aaaacgtccg cttgttttac tcaaatcaag cattcttcat cttattgata aaataagaaa
     6301 ccatttcaga agctatatca acatcattta ctctctctat gccttcaaat cctgggctgg
     6361 cattgacttc taaaataact acgcctttct ctgtccgcag aaaatcaacg cctgcaatat
     6421 caagccctat cgttttagtt gctttaattg ctaacgcacg ttccatctga gttaattcaa
     6481 tcggttgagc cgttccacct tgatgaatat tggctctaaa atcgcctgtt acgctggttc
     6541 ggctcatggc agcaaccact tcattgccaa tcacaaacgc ccgaatatct tgccctttag
     6601 cgtcgcccac aaattgttgg catagataag gctcatttac ttgcctaaag gtcgctaaca
     6661 cggcctcggc attatttttg ccttcaaata gcattacgcc attcccctgt gaaccattta
     6721 gcacttttga aataagtgga aaagcaaatt gattgagttg tgatttcaca gaaaccaaat
     6781 gccctgcaaa attcgtatta ggaaccggca aattgtgttt ggctaatttt tgtaatgttc
     6841 gccatttgtc tcgggctaac gcaaatgccg ctgaatgatt tagaacagga atacctttcg
     6901 cctcaaaatg gcgtaacacc gaacagccca tttcggtact gcctgtgcca aaacggggta
     6961 aaatgccgtc ataattcggc aacaataccg gctccggacg gttgttatca tagctctcgc
     7021 cagcttgata gtagatctta aattcgccat tttctaaccc gagtagcatt ctattctggt
     7081 ctaaaatatc cagctcaatc ccctttgcct cacaagcggc ttttattcga cagcagctat
     7141 ataaacgagg ctctcgacat agcattagcc acttcatttt ttcagcaatc ctttctgatt
     7201 gaaaaacggc aaaatatgcg gacgaacagg cttatccaat gttttatgaa tagctgcttg
     7261 ccattgttgt ggctcgccga ttctggcttg gtagtaagcg gtcaaatttt cattatattt
     7321 tgcaaattca gtccaatcaa aatcctgata ttgattgtta taacaaacca aactattcgg
     7381 caaacgaggt tttattgccg gctcttgtgc cggatacccc aaacacatac ccaccagtgg
     7441 aacacaatat tccggcaagt taagggcttt agaaacccgc tcaatatcat tacgcaatgc
     7501 tccaatatac accgctccca gccctagtga ttcggcggct aaaaatacat tctgggccaa
     7561 aataccggta tctaccgccc caatcaacga cacctccgcc cattccaact gcgattcagg
     7621 gacaagttgt ttgtgtttat taaaatcaat gcagaacacc aaaaattcag cacaatctac
     7681 tacatattgc atacctgaaa cggcttttaa tgtttctcga atctgccgat cggttacacg
     7741 aatcaccgtc acacattgca gatgatttga acttgaagcc gctttacctg cctcaattaa
     7801 gatttggaaa atctcttcag aaataggctg ttcggtaaat ttacgaatag aacggtgaga
     7861 taaaatcgtc tcaagagtcg gtttactttg catatttttc tcgtctaaag tttatctaaa
     7921 taatttttgc catcaagcaa acttacttgt cgcataatcc acgcttggcg ttttcgcata
     7981 ctcgggccgg gtttatctac cctaaacacc aacgggttag gcaaagaggc tgccaatagt
     8041 gcggattctt gcaaggttag ctgtttggct gattttttga aaaaatgctt tgcggctgct
     8101 tccaccccga aaatgccgtt accaaattcc gcaatattga ggtacacctc taaaatccga
     8161 tccttactcc acacattttc gactaacagg gttaaaggca cttcaatccc tttgcgtaac
     8221 cagctttgcc cgtgccacaa aaacatattt tttaccgttt gttgcgaaat ggttgaacct
     8281 ccacgcactt ttttggattt agcattcagt ttcaaggcag tttctatcgc ttgtaagtca
     8341 atgccaaaat ggctttcaaa cttttgatct tctgaggcaa tcacagccat ttgcatttgc
     8401 cacgaaatct cattcaggct cacccattct tttttgatcg ggtaagattt cccctgaacc
     8461 agattctcca cctttttttg caccatatac gcagaataag gcaccggtaa caccgaaaaa
     8521 atcagtatcg gaatcaggaa aaatgtgcca ccaaaggtta ataagcgact agaaccaaac
     8581 caaagccagc ccagtttact ttttattttg cgaggaataa aaaaaagccc tattttcttc
     8641 aaaagagagc ttaaaaatcg cttgttacgc tttgccattg actgttccat taaccttgcc
     8701 gttcagccat tgcagaaaat cttggtcgag tgttttaagc atatttgaac cacgagtgat
     8761 ggttgccgca ctggtattta aattttgttg gatttcccgt tgcgaatctt caccttttag
     8821 caaagcttgc acaatttgta aacgcaagcc taatgaacct cgctcatcgg cggtgagaaa
     8881 cattgagaaa aacggctcaa gtttatcttc cgcaaccgct tgttcaagca gagccaaaaa
     8941 ctgcgtccac tcgtttggat ctcgctggtt atagagtgtt ttcatactgt cccttgaagt
     9001 aaaaacggtg acaagcggtc agatttttac aaaattttgc agaaaaacga ccgcttgctc
     9061 tccattattt tttcgatttt ttaccgccac gttggtcgtc taactctgcc cctttcggta
     9121 cgctgaattt aaacaaatcg cttgagacac cgccggtagt aatattgcgt aacacatata
     9181 agttactttg cccgtcacgc tcaatggtac tgaaaccttt taataaaccg ctttggtcaa
     9241 tccgcacatc aaactgtttt aaattgcttt ttttcgattt tggcttaagc acgaaagtat
     9301 cagcactctg gctgacatca tattgatccc agtggctttt atcaccgctg gttaataaga
     9361 cgaatggcgt attattaact gcatcttgca cggtatttac cgtaacttgt gaaacgaaag
     9421 gatcgtaaaa ccataaatct ttgccgtcag atacaatcaa attttcttgt ggtgatttgg
     9481 tatccattct aaataagttc gggcgtttta cttggaattt gcctttgcct tgctgaattt
     9541 gtttaccttt gctagaacgc acggtttggt cgaaatccgc actatattgg cttacttgtt
     9601 ccaaacggcg ttgtaattct gcaacagatt gagcatcagc caatgccaca tttgccaagc
     9661 caaataacgc taaaccgaat accgattttt taaataatgt tttcatttgt tttccttttt
     9721 accaacaaga attttcttgt ttgacaataa aatcatcaaa agtttcatat taatgaactg
     9781 ttacaacaat acaataaaag tcgtgaattg tatagtttaa tgagcttcgt cccagttttc
     9841 gcctacgcca acttcggcaa tcagtggcac tttgaggcta atcgcttttt ccatttcggt
     9901 tttgattaat tggctgtaat ggtcgatttt atcggctttg acttcaaaca ccaattcatc
     9961 gtgaacctgc ataatcattt tgatttcatc attatcacga atcgcattat caataccaat
    10021 catcgccacc ttaataatat cggcagccgt gccttgcatt ggggcgttga ttgccacccg
    10081 ttctgctgct ttacgcaaaa tagcattgcc ggatttaata tcaggcaagt acaaacggcg
    10141 accaaatagg gtttcaacat agcctttttc aactgcaact tcacgaatat cggtcataaa
    10201 ttgttgaacc gccgggtaac gttggaagta gagatccata tatttttgag cctctgcacg
    10261 cccaatccct aattggtttg aaagcccaaa tgacgacatt ccgtaaatca gcccgaagtt
    10321 aattgctttc gcactgcggc gttgttcgga ggtgacttca cctaactcca ccccgaaaat
    10381 ttcggcagcg gtagcacggt gaatatcttt gccctccgca aaggcgttaa tcattccctc
    10441 atcattcgat aaatgagcca taatccgtag ctcaatttgt gaatagtcgg cagcgacaat
    10501 tttataacct tctctcgcga caaaagcttg gcgaatccgt ctgccctctt cattgcggat
    10561 cggaatgttc tgcaaattcg gatcgctgga agaaaggcgt ccggttgcgg tgactgcttg
    10621 attgtaagag gtatgcaccc gccctgtttt tttgttaatc agttgtggta atttatcggt
    10681 gtaggtagat ttcaacttac ttaacccacg atgctccatt aataacttcg gcactaaatg
    10741 cccttgttga gccaactcgt ctaacacttc ttcgttggtt gagggtgcac cttttggagt
    10801 tttcttcaac accggcaagc ctaatttttc aaacaaaatc tcttgtaatt gtttagttga
    10861 ggcaagattg aaggtttgcc ctgcttctgc gtgtactaat gtttcaactt cctccaaacg
    10921 tttttcaatt tcagccgatt gggtcaataa ttttttcggg tcaatcaaca cgccattgcg
    10981 ttcaattcga gaaagtacgc ccacaagtgg catttcaatt tcatcgaata acttcaccaa
    11041 ttcaggctct tgctgtagtt tttcccacaa tacttggtgg agtttcatcg tcacatcggc
    11101 atcttctgcc gcatattcag ccgcttttgc tatctcgatt ttatcgaagg tcagttgatt
    11161 tttcccctta ccagcaagct cttcaaacgg aatcgtttta tgccccaaat aacgggcggc
    11221 taaatcgtcc atattatgac gaccggtgct gttttgggtg taagattcaa tcatcgtatc
    11281 aaaagcaaca ccttgtagct caatgccgta gttcgcaaaa atggttaaat catatttaat
    11341 attctgcccg atctttttaa tctccgggtt aagcaaaatc ggcttgagtt tacttaaaca
    11401 agcggtcaaa tttagctgat tttttaccaa ttcatcggaa gacgggcttt ctgaacgctc
    11461 ctcatcaccg aacatatcgg cttgttgcgg ttgggctttt tgcttatgcc caagcggaat
    11521 atagcacgcc tcgccatttt ccagtgcaaa ggaaataccg accaaattag ccgacattgc
    11581 gtcaaggctg tcggtttcgg tatccaccgc aatcaaacga gctgcggtga gtttttcgat
    11641 ccaggctgat aatttctgtt cagtatcaac ggtctcatat ttgctacgat cgatctcgac
    11701 cacatttgca acattttcct catttctgac cgcttgtgtt acttggtaat tattcggtac
    11761 tttttcagcc gaagtttgag tgaccggatt ggcattttga ttcacttcgt tcagccagcg
    11821 tttaaattca taatgctcaa ataattgagc aagctcagca cggtcttgcg gctgtgttaa
    11881 aagttgatcg tgagtaacat caagttccac atcggttttg atggtagcaa gcaaatagga
    11941 taaatcggca ttcgcttttt ccgcttccaa ttttggggcg aaattttttg ccccacggaa
    12001 agaaagggtg gcaatttgat ccaaattagc ataaatctgt tcaagtgaac caatgccttg
    12061 caataaagct aatgcggtct tttcccccac accttttacg cccggaatgt tatcggcgga
    12121 atcgcccatt aatgccagaa agtcgataat caactcaggc ggaatgccat atttttcaat
    12181 cacgccttca cgatctaaca aagtattgtt catcgtgtta atcaacataa tgtgatcgtt
    12241 caccaattgt gccatatctt tatcgccggt gctgatcaac acatctttgc cgtctttagc
    12301 ggcttgcaca gcaagcgtgc cgatcacatc atcagcttcc acaccttcaa tcgaaatcag
    12361 tggaatgcct aaggctttga taatgcgatg caaaggctca atttgagggc gtaaatcatc
    12421 gggcattggt gggcggtgcg atttatattg cttaaacaac tcatcacgga aggttttccc
    12481 tttggcatca aacaccacag caatatggct tggattcact tgtgaaatca ggctttttaa
    12541 catattcagc acgccataca ttgcccctgt cggttcgcct aagcgattag tgagcggagg
    12601 gaaagcgtga aatgctcgat ataaatagga agaaccatcc accaatacca gtggattttt
    12661 tgcgatagtt gccataaaaa tctctttata atcaaataaa tattggcgtt attatacaag
    12721 aatatttaag aattgagtgt gtat
//
LOCUS       DANLZC010000071        12559 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.071, whole genome shotgun sequence.
ACCESSION   DANLZC010000071 DANLZC010000000
VERSION     DANLZC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12559
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.071"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..795
                     /locus_tag="PXE44_001518"
     CDS             <1..795
                     /locus_tag="PXE44_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589023.1"
                     /translation="FKAITKFVKVDRDTVFEFFNISLHNPRKNGKNRICSPREFREYR
                     KLKKPDLFNDNPAFFINKGQKGIPQGSPISALLSNIYMLEFDVLVKEKIMECKGSYFR
                     YCDDILCIIPNEYEEFILDYITGEIKSKLKLEINKDKTEVVKFQYCNRTKKIINEKKL
                     QYLGFILHNNSISIRSSAFTRYSNKMKRGVSLAKQTQGKYNRIRIRRGVAIKGIYKRK
                     LFSKYSHFGKSNFISYGKRAYSSMDSKVIKNQLKPLWYRLKKEIYL"
     gene            complement(1047..2327)
                     /gene="hemL"
                     /locus_tag="PXE44_001519"
     CDS             complement(1047..2327)
                     /gene="hemL"
                     /locus_tag="PXE44_001519"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDL5589024.1"
                     /translation="MSISEQLFEQAQKVIPGGVNSPVRAFKGVGGTPVFIEKAEGAYI
                     TDSDGKQYIDYVGSWGPMVLGHNHPAIIDAVLKAVPKGLSFGAPTAAEITLAELVCKL
                     VPSIEMVRMVSSGTEATMSAIRLARGYTNRDKIIKFEGCYHGHSDSLLVKAGSGALTL
                     GQPSGPGVPADFAKHTLTCTYNDLDSVKAAFEQYPNEIACLIVEPVAGNMNCIPPQEG
                     FLQGLRDLCTQYGAVFIIDEVMTGFRVALGGAQSYYGVTPDLTTLGKIIGGGMPVGAF
                     GGKKEIMEYIAPTGPVYQAGTLSGNPIAMAAGLACLTELSKAGNETILAEKTKTLAEG
                     FKALADKHNVPLTVQYVGGMFGLFFTEQKAVNNFQEVMKCDAAKFNRFFHLMLEQGVY
                     LAPSAFEAGFMSLAHSDEDIARTLEAADKAFAQL"
     gene            2469..3533
                     /gene="wecA"
                     /locus_tag="PXE44_001520"
     CDS             2469..3533
                     /gene="wecA"
                     /locus_tag="PXE44_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDL5589025.1"
                     /translation="MWLTFLTVFIVSFLALIVMRPVAEFVGLVDKPNFRKRHQGLIPL
                     IGGIALFIGNLTFYFMQWQDMRLPELYLTAVTILLIIGVLDDRFDISPLLRAGIQAAL
                     AGAMIYSGLSLSSLGQIIAPFSIELGWLGIVFTVFITIGVINAFNMVDGIDGLLAGLS
                     SVSFAGLGTLMFINGEPSLGYWCFALIFVLLPYAMFNLSLFGAKWKVFMGDSGSMLIG
                     FTIIWILLLSTQGQGSPISPITGLWLIAVPLIDMVAVIIRRLKKGKSPFKPDRLHLHH
                     LMMRAGLTSRQALLVITLWAGFCSTIGVLGEVYYWNQWVMMLMFIGLFFLYAYSITHA
                     WRVTRFVRRLKRRAKRSKQL"
     gene            3544..4356
                     /locus_tag="PXE44_001521"
     CDS             3544..4356
                     /locus_tag="PXE44_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS chain length-determining protein"
                     /protein_id="HDL5589026.1"
                     /translation="MKTLFYRLLVLILFAAIGAGAGYGLSYTQPEKWKVTAQFEQPTI
                     PDLGNYYSLLSTYHFLNSNSQEANSQTAQQQASEQVYAEFKRNLTSADLLQAYLLQTE
                     AVKLKAQVAKKSPAVVAAELVEQFRFEKDMATSADNVSLIAENAEEAQKLLADFIPLV
                     NTQARDKLNADLIAKWKVLFQQIKTASELNINGKQGSQDWAAKLQMMRSVQPLDNQLT
                     AYRLVKSPSVPLKSESPDPPILADDWGIKWLTAGLYAQFCGESESSTCGTLI"
     gene            4397..5644
                     /gene="wecC"
                     /locus_tag="PXE44_001522"
     CDS             4397..5644
                     /gene="wecC"
                     /locus_tag="PXE44_001522"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL5589027.1"
                     /translation="MMSKTEFNTICILGLGYIGLPTSVVFANAGKQVIGVDINPQVVA
                     SLNQGNIHIIEPDLQTAFTQAVKKGNFFATAKPQPADAFIIAVPTPLCGKQPDLTYIQ
                     NAAAMIAPCLRKGNLVVLESTSPVGTTENLAEWLSQARPDLAFPADVSIAYCPERVLP
                     GNIMTELVENDRIIGGLSEQCSQQAVDLYQIFAKGRCVTTTARTAEMCKLVENSFRDV
                     NIAFANEISMICDALKINVWELIELANLHPRVNILQPGAGVGGHCLAVDPWFIVSTSP
                     QQARLIRTAREVNDSKPQWVIEQVKTSVADCAMEQDCKPSELTIACLGLAFKPNIDDL
                     RESPALAITRQLAAWHQGSLLAVEPNIQQLAGEKITLVDLNTALSQADILVLLVDHTS
                     FKQISREQIRQRHIIDCRGVWVR"
     gene            5641..6285
                     /gene="rffC"
                     /locus_tag="PXE44_001523"
                     /gene_synonym="wecD"
     CDS             5641..6285
                     /gene="rffC"
                     /locus_tag="PXE44_001523"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDL5589028.1"
                     /translation="MKPDQWLSDFFGREIVQVKVAADDFRQIARLQKQGFVLVEGELE
                     FELALATFSENMTACRPADMADLPDLNLLFGSAFPDSRFRSPYFSREENQRFYRTWIE
                     NAVKGKFDDIGLVQCNADGQIQGAVTVRLLAQQQAKIGLLAVKPDCRQQGIGSELLNA
                     AIAWAKQQNAKKLMITTQLSNQKAIRFYQAFGASIKAVYYWFYPALEKYDDEQS"
     gene            6272..7415
                     /gene="rffA"
                     /locus_tag="PXE44_001524"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /pseudo
     CDS             6272..7415
                     /gene="rffA"
                     /locus_tag="PXE44_001524"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
     gene            complement(7459..8880)
                     /gene="glnA"
                     /locus_tag="PXE44_001525"
     CDS             complement(7459..8880)
                     /gene="glnA"
                     /locus_tag="PXE44_001525"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL5589029.1"
                     /translation="MSNAIANVSKLIQDNDIKFVLLKFTDIKGKEHGVSLPVSLVAND
                     LEDLFEEGKMFDGSSVEGWKAINKADMLLMPIAETAVVDPFARIPTLSIRCSIYDPNT
                     MQSYDRDPRSIATRAENYLKSTGIADSVMFGPEPEFFLFDDVRFSTEMNNVSYRIDDV
                     EAAWNTNRKFEEGNKAYRPLKKGGYCAVAPIDTAHDIRSEMCLLMEEMGLVIEAHHHE
                     VATAGQNEIATRFNSLTLKADETQIYKYIVQNVAVEHGKTACFMPKPFAGDNGSGMHC
                     NMSLSKDGKNVFMGDKYAGLSETALYYIGGIIKHAKALNAFTNPTTNSYKRLVPGFEA
                     PVLLAYSASNRSASIRIPAVTSPKATRVEARFPDPMANPYLCFAALLMAGLDGVINKI
                     HPGDAMDKNLYDLPPEELKDIPTVASSLESALDALSNDFEFLTQGGVFTKDFIDAYIG
                     IRRKEVERVNMTPHPVEFELYYS"
     gene            9067..10137
                     /locus_tag="PXE44_001526"
     CDS             9067..10137
                     /locus_tag="PXE44_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDL5589030.1"
                     /translation="MLPIYHILGSEIEHHNQQVLTFFQQELLPLLPDSEHFFYVVGKP
                     SLQTAFPHLRIKQFSSQKAIANALIAKKRQNPTACFLLHGQFNPWIWLAIALGRLPTT
                     NLIWHIWGADLYEMATSWKFKLFYPFRRVAQQKILQVWATRGDLDHLWRNMRTRSEQD
                     HLIYFPTKLGDQIKISPTQNDRLTILLGNSGDPANELIQALSEIHQRLGNNVRIIIPM
                     GYPENNESYIRQVEAYSKRLFLAENLQILRKKLSFEEYVTLLSHCDLGYFNFKRQQGI
                     GTISLLISHNVPCVLHSANPFGLDLQAEKVPYLLAENLTASQIQTTKQQLKSIDKNRI
                     AFFAPGYTKLWQQRLIELMQNE"
     gene            10130..11401
                     /locus_tag="PXE44_001527"
     CDS             10130..11401
                     /locus_tag="PXE44_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WzyE family oligosaccharide polymerase"
                     /protein_id="HDL5589031.1"
                     /translation="MNNLIPLGGFYLVSVGLFFSLLYQLYQKSGISFHLLFSLLYFVT
                     FYAGFPFSLIMAFGFDYPLPELKTQWQVFTAAGVGYLIYYVVYSRLWSAVSQPTALVA
                     NFQAKTTACILAAVAVVALLYFLYLNGFLLFKLEKYSQLFAKDRVILSALKRFFYFLL
                     PALLIFCFLYKNKTAWWALLIVGGAFGILSYVAVGGTWANLALAVGFFLLIGLQQGYL
                     SLSKVIAVSLIGVVAMFFLALARYKLTLSGSEMIYTFLYLSRDTFSPWENLARVFSNE
                     VEYQGLMPIVRDFYVYIPQSLWQERPEIVWNTANYFTKVILGNQSGLAISPTLLGSFY
                     IMGGFSVMALGMGIVAAMIKALDNLFAYAKACNSALLQAYCFGNLFNLIVLVREGIDA
                     FVSRFCFFTLIFMACYLLAKILSMGKNNNVR"
     gene            11391..12113
                     /gene="rffM"
                     /locus_tag="PXE44_001528"
     CDS             11391..12113
                     /gene="rffM"
                     /locus_tag="PXE44_001528"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDL5589032.1"
                     /translation="MFDKVIIRGIALQAVKNREIFANWLMNEKRIQFGRLVAINAEKV
                     ILSEKNPLLRRVIDEAEFNYADGISVVQSIRKKYPNYKIERIAGADLWETLMQRAGAL
                     NVPVFLVGSSADTLTKVEAKLAQWKVNIVGRQNGYFKAEQEQDVIEQIKASGAKFISV
                     AMGTPKQELFIQQLQQQYPQALYMGVGGTYDVFAGKVKRAPKIWQNLGLEWLYRLLKQ
                     PTRWQRQLNLLKYAYYYLTNQL"
     gene            12272..12348
                     /locus_tag="PXE44_001529"
     tRNA            12272..12348
                     /locus_tag="PXE44_001529"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12306..12308,aa:Arg,seq:ccg)
     gene            12378..12453
                     /locus_tag="PXE44_001530"
     tRNA            12378..12453
                     /locus_tag="PXE44_001530"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12411..12413,aa:His,seq:gtg)
     gene            12478..12554
                     /locus_tag="PXE44_001531"
     tRNA            12478..12554
                     /locus_tag="PXE44_001531"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12512..12514,aa:Pro,seq:tgg)
BASE COUNT         3454 a         2490 c         2885 g         3730 t
ORIGIN      
        1 tttaaggcta ttactaaatt cgtaaaagta gatagagata ctgtatttga attctttaat
       61 atctcccttc ataatcctag gaaaaatggg aagaatagaa tttgtagccc tcgggaattt
      121 cgagaatata ggaaattaaa gaaacctgac ttatttaatg ataatccagc tttttttatt
      181 aataaaggcc agaaagggat tcctcaaggt tctccaataa gtgcattgtt atccaatatc
      241 tatatgttag agtttgatgt gttagttaaa gaaaagatta tggagtgcaa agggagttat
      301 tttcgttatt gtgatgacat tctatgtatc attcctaatg aatacgaaga atttattctc
      361 gattacatca ctggggagat aaaaagtaaa ttaaaactag agataaataa ggataaaact
      421 gaggttgtta aattccaata ttgtaataga acaaaaaaaa taattaatga aaagaagtta
      481 caatatcttg gttttatatt gcataataac tctattagta ttagatcatc ggcatttaca
      541 cgatattcaa ataaaatgaa acgaggggtt agtctagcta agcaaactca gggtaaatat
      601 aataggataa gaataagacg aggggtagct atcaaaggca tttataaaag aaagctattt
      661 tctaagtatt ctcatttcgg aaaatctaat tttatctctt atggaaagag agcttattca
      721 tctatggatt ccaaagttat aaagaaccaa cttaaacctt tatggtatag attaaagaaa
      781 gaaatatatt tgtaatatta gaaagtgtaa gacttgtcct tgcctgcaag tgttggaagc
      841 ccatatattg ctttatcaat cctattccct cgatagcgcc cgtctccaga cgggtgcttg
      901 tttaagcata atgaaagttt gtataagcac tcgtcagaga cgaacaccat cataattgct
      961 atcgtaagag aaatttgcac tacaagcggt caaattccct caaaaacttg caaattattg
     1021 agtaaaccca accgcttgtc attcttctaa agctgagcaa atgccttatc cgcagcctct
     1081 agcgtacgag caatatcttc atcactgtga gctaaagaca taaaccctgc ctcgaatgcc
     1141 gatggggcta aatacacgcc ttgttccagc attaagtgga agaaacggtt gaatttggcg
     1201 gcatcacatt tcattacttc ttggaagttg tttactgctt tttgttcggt gaagaataag
     1261 ccgaacattc cgccgacata ttgtaccgtt aatggcacat tgtgcttatc tgcaagagct
     1321 ttaaagcctt cagcgagggt tttggttttt tccgctaaaa tggtttcatt tcctgctttt
     1381 gataattcag ttaagcatgc caaacctgca gccattgcaa tcgggttgcc tgagagcgtg
     1441 ccggcttggt aaaccgggcc agttggagcg atgtattcca taatctcttt tttaccacca
     1501 aatgccccca ccggcattcc gccgccgata attttgccga gtgtggttaa atccggtgtt
     1561 accccatagt aagattgagc cccaccgagt gcgacacgga agccggtcat tacttcatca
     1621 ataataaata ccgcaccata ttgtgtgcaa agatcacgta agccttgtaa aaagccttct
     1681 tgtggcggaa tgcagttcat attgccggca acaggctcaa caattaaaca agcgatctca
     1741 tttgggtatt gctcaaatgc tgcttttact gaatctaaat cattataggt gcaagtaagc
     1801 gtatgcttgg cgaaatcagc gggtacgccc gggccgcttg gctgaccgag ggttaaagca
     1861 cctgaaccgg cttttactaa cagagaatcg gaatgcccgt ggtagcagcc ttcaaatttg
     1921 atgattttat ctcggttggt atagccacga gccaaacgaa tggcagacat tgttgcctct
     1981 gttccgctgc tcaccattct caccatttca atagaaggta ctaatttgca cactaattcg
     2041 gcaagtgtga tttctgctgc cgttggggca ccgaaactta agccttttgg cacggctttt
     2101 agcaccgcat caataatcgc cgggtggtta tgcccgagta ccattggccc ccaagagccg
     2161 acatagtcga tatattgttt gccgtcgctg tcggtaatat aagcaccttc ggctttttca
     2221 ataaaaaccg gtgtgccgcc cacgccttta aatgcacgca caggggagtt cacaccgccc
     2281 ggaatcactt tttgggcttg ttcaaacagt tgttctgaaa tagacatatc aattcctttc
     2341 ttttgatgta aaaatttgcc tgttattgta ctgaaaagcc cttgttttcg ctaaaaaatt
     2401 tacgattttg ttgccttatg ctattctgca ttcctatttt atgactcatt ttttgaggca
     2461 atccctgtat gtggcttact tttttaaccg tatttattgt ttctttttta gccctgattg
     2521 tgatgcgtcc ggtggcggaa tttgttggtt tggtagataa gcccaacttc cgtaaacgcc
     2581 atcaggggtt gattccgtta atcggtggta ttgcattatt tatcggcaat ctcactttct
     2641 attttatgca gtggcaagat atgcggctgc cggaactgta tctgacagcg gtaactattt
     2701 tgctgattat cggcgtgtta gatgaccgat ttgacatcag cccattactg cgtgccggga
     2761 ttcaagcagc actagctggg gcgatgattt acagtggctt gtcgctcagc agtttagggc
     2821 aaattattgc cccctttagt atagaattag gttggctcgg cattgtcttt accgtgttta
     2881 tcaccattgg cgttattaat gcgtttaata tggtagatgg gattgatggc ttattagcag
     2941 ggctttccag cgtgagtttt gccggtcttg gcactttaat gttcattaat ggcgaaccaa
     3001 gtttgggcta ttggtgcttt gcccttatct ttgtgttgtt gccgtatgca atgttcaatt
     3061 tgagcctgtt tggggcgaaa tggaaagtgt ttatgggcga ctcaggcagt atgctgatcg
     3121 gctttaccat tatttggatt ctgctgttaa gcacccaagg gcaaggctcg ccaattagcc
     3181 cgattaccgg tttgtggctg attgccgtgc cgttaattga tatggttgcg gtgattatcc
     3241 gccgtttgaa aaaaggtaaa agtccgttca aaccagaccg cttgcaccta caccatttaa
     3301 tgatgcgagc agggcttacc tctcgccaag cattattggt gattaccctc tgggcgggat
     3361 tttgttccac cattggggta ttaggcgagg tgtattattg gaatcagtgg gtgatgatgc
     3421 taatgtttat cgggctgttt ttcctatacg cctattccat tacccacgct tggcgtgtaa
     3481 cccgttttgt tcgccgtcta aaacgccgtg caaaacgtag caaacaactt taaaggatat
     3541 gttgtgaaaa ccttatttta tcgactttta gtgctaattt tatttgctgc tatcggggca
     3601 ggtgccggtt atgggctgag ttatactcaa cctgaaaaat ggaaagtgac cgctcaattt
     3661 gagcaaccga ctattcccga tttaggtaat tattactcgt tactttccac ctaccatttt
     3721 ttgaatagta atagccaaga agcgaatagc caaacggcac agcaacaggc aagtgagcag
     3781 gtttatgcgg aatttaaacg caacctcacg tccgccgact tgcttcaagc ctatttgcta
     3841 caaacagaag cggtgaaatt aaaagctcag gttgcaaaga aatcaccggc ggtggtggcg
     3901 gctgaattag ttgaacaatt ccgttttgaa aaagatatgg caacttcggc agataatgtc
     3961 agcttaattg cagaaaatgc ggaagaggct caaaaattat tagccgattt tattccgttg
     4021 gtaaacaccc aagctcgaga taaattaaac gccgatttaa ttgcgaaatg gaaagtcttg
     4081 ttccagcaaa ttaaaactgc ttcggagttg aatattaacg gtaagcaagg ctcacaagat
     4141 tgggcagcta aactgcaaat gatgcgttct gttcagccgt tagacaacca acttacagct
     4201 tatcgcttgg ttaaatcgcc tagcgttcca cttaaatcag aatcgcctga tccgcctatt
     4261 ttggctgatg attggggcat taagtggctt actgctgggc tttatgctca gttttgcggt
     4321 gaaagcgaaa gctcaacctg cggaacttta atctaaaatg gcacacaatt tagtgtgcca
     4381 tttctctttg taactgatga tgagtaaaac tgaatttaac actatttgta ttctggggtt
     4441 aggctatatc ggcttgccga cctcagtcgt ttttgccaat gccggcaagc aagtaattgg
     4501 ggtggatatt aatccgcaag tggtggcaag ccttaatcaa ggcaacattc atattatcga
     4561 gccggattta caaactgcct tcacacaagc ggtcaaaaaa ggtaattttt ttgcaactgc
     4621 caaaccgcaa ccggccgatg cttttatcat cgccgtgcca accccacttt gcgggaagca
     4681 gcccgatctc acttatattc aaaatgccgc cgctatgatt gccccttgtt tgcgtaaagg
     4741 gaatttggtg gtgctggagt ccacttcgcc tgtcggtaca acagaaaatt tagccgaatg
     4801 gctaagccaa gctcgccctg atttagcctt tccagccgat gtgtctattg cctactgccc
     4861 tgaacgtgtt ttgccgggta atataatgac ggaattggtt gaaaatgacc gcattatcgg
     4921 cggattaagc gaacaatgca gtcaacaagc ggtcgattta taccaaattt ttgcaaaagg
     4981 acgttgtgtc accaccacag ctcgcacagc ggagatgtgc aagttggtgg aaaacagctt
     5041 tcgtgatgtg aacattgcct ttgccaatga aatttcaatg atttgtgatg cgttgaagat
     5101 caatgtgtgg gagctaattg aattggcaaa tcttcacccg agagtaaata ttctccagcc
     5161 cggagcaggg gtgggcggac attgtttggc ggttgacccg tggtttatcg tttccacatc
     5221 gccccaacag gctcgcctta ttcgcacggc acgagaagtg aatgacagca aaccgcaatg
     5281 ggtaatcgag caagtgaaaa catcggtcgc agactgtgcg atggagcaag attgtaagcc
     5341 aagtgagcta accatcgctt gtttagggct agctttcaag ccgaatattg atgatttgcg
     5401 ggaaagcccg gcattagcga ttacccggca acttgccgct tggcatcagg gtagcctgtt
     5461 ggcggttgag ccaaatattc aacagctggc tggcgaaaaa atcaccttag tcgatctcaa
     5521 caccgcactt tcgcaggcag acattttagt cttgttagtg gatcacactt catttaagca
     5581 aatttcaagg gaacagatca ggcaacggca tattattgat tgtcgaggag tgtgggtaag
     5641 atgaagccgg atcaatggct gtccgatttt ttcggtagag aaattgtgca agtaaaagta
     5701 gcggctgatg attttaggca aatcgctagg ttgcaaaaac aaggttttgt acttgtggag
     5761 ggagaattgg aatttgagct ggcacttgca acattttctg aaaatatgac cgcttgtcgc
     5821 ccggcggata tggcagattt gcccgacttg aacttattgt ttggttcggc atttcccgat
     5881 agccggtttc gttcgcccta tttttcaagg gaagagaatc aacgctttta ccggacttgg
     5941 attgaaaatg cggtaaaagg caagtttgat gatattggct tggttcaatg caatgctgac
     6001 gggcaaattc aaggggcggt aacggttcgc ttacttgccc aacagcaagc gaaaatcgga
     6061 ttattggcgg taaagcctga ttgccgacaa caaggtattg gctcggaatt gttaaatgct
     6121 gcgatagcgt gggcgaaaca gcaaaatgcg aaaaaactga tgattaccac tcagctgagt
     6181 aaccagaaag cgattcgttt ttatcaagcg tttggtgcaa gcattaaggc ggtgtattat
     6241 tggttttacc ctgctttgga aaaatacgat gatgaacaat cctgatttta ttccgtttaa
     6301 tcaacccccg gttgtggggg cggagcaagc ctatatagaa caggcgatgg caacagggca
     6361 gctttcgggc ggtggtaaat tcacccgctt atgtgagcag tggcttgaaa acctggcccg
     6421 tagcaaagtg ttgcttaccc cttcttgcac ggctgcgtta gagatggcgg caatactgct
     6481 ggatattcag ccggacgatg aagtgattat gccgagctat acttttgtct ctactgcaaa
     6541 tgcgtttgcg ttgcgtgggg caaagattgt gtttgtggat attcgtccgg atacaatgaa
     6601 tattgatgaa cgcaaaattg agcaagccat taccccaaaa accaaagtga ttgtgccggt
     6661 gcattatgcc ggcattgctt gtgatatgga ttcaataatg gcaacagccg aaaaatacgg
     6721 gctttatgtg gtagaagatg cggcacaggc agtcaattcg ttttataaag gtagggcgtt
     6781 aggttccatc gggcatattg gttgctatag ttttcacgaa accaaaaact ataccgctgg
     6841 cggtgaaggt ggggcgatcg tgattaatga ggcatttgct ggaaaaagcg gaaatcatca
     6901 gagagaaagg cactaaccgt agccaatttt ttcgtggtga ggtagataaa tatcgctggc
     6961 aggatattgg ttcaagtttt ttaatgtcgg aaattcaagc ggcttatttg tatgctcagc
     7021 ttatagaggc agataaaatc aaccaacagc gtttaaccct ttggcacaat tactatcaag
     7081 cctttttgcc acttgcccaa acagctaagt gcgagcttcc tcgcattggc gaatattgtc
     7141 gccataacgg gcatttgttc tacttaaagc taagtgactt gaagcagaga actgctttta
     7201 ttgattttat gaaggcacag aatatcttaa ccgtgtttca ttatgtgccg ttgcacagca
     7261 gtccggcagg gcagaaatta ggtcgattag caggtgatga tcgctttact accttagaaa
     7321 gcgaacggtt ggtaagattg ccactgtttt ataatttgag taagcaggag caagagcgtg
     7381 ttattcaagc agtgcttgct ttcttcaatc aataaaaaac ggctgagtgt ttcagccgtt
     7441 ttttgctata tgttgttact atgaatagta aagttcaaat tctaccgggt gcggagtcat
     7501 atttacacgt tccacctctt tgcgacgaat accgatataa gcatcaataa agtctttcgt
     7561 gaacacgcca ccttgtgtta agaattcaaa atcattgctt aatgcgtcaa gagcggattc
     7621 taaagacgag gcaaccgttg gaatatcttt taactcttcc ggcggtaaat cgtataagtt
     7681 tttatccatt gcatcgcccg ggtggatttt gttgatcaca ccgtctaagc cagccattaa
     7741 cagggcggca aagcaaaggt atgggtttgc cattggatcc gggaagcgtg cttctacacg
     7801 ggtcgctttc ggactggtca cagccggaat acggatagag gcagagcggt tacttgcaga
     7861 atatgccaat aacaccggtg cttcaaaacc cggcactaaa cgtttataag agttcgtggt
     7921 tgggttagta aaggcattta atgcttttgc gtgtttaatg ataccgccaa tatagtagag
     7981 ggcagtttca gataagccgg cgtatttatc gcccataaac acatttttgc cgtctttgct
     8041 taatgacata ttacagtgca ttcccgagcc gttgtcgccg gcgaacggtt ttggcataaa
     8101 gcaagcggtt tttccgtgtt ctacggcaac attttgcacg atgtatttat agatttgggt
     8161 ttcatcggct tttaaggtca ggctgttgaa tcgggttgcg atctcatttt gtccggctgt
     8221 cgcaacttcg tggtggtgag cttcaatcac taaacccatc tcttccatca ataaacacat
     8281 ttcggagcga atatcgtggg cggtatcaat cggtgcaacc gcacagtagc cgcctttttt
     8341 caacggacga taggctttgt tgccttcttc aaatttacgg ttggtattcc aagccgcttc
     8401 tacatcgtca attcggtatg acacattgtt catttcggtg ctaaaacgca catcatcgaa
     8461 taagaaaaat tcaggctcag gtccaaacat tacgctatcg gcgataccgg tggatttcaa
     8521 gtagttttcg gcacgggttg caatagagcg agggtcacgg tcataagact gcattgtgtt
     8581 tgggtcgtaa atggaacaac gaatagaaag ggtaggaata cgggcgaaag ggtcaactac
     8641 agcggtttca gcaatcggca ttaacaacat atctgctttg ttaattgcct tccaaccttc
     8701 tacggacgaa ccgtcgaaca ttttgccttc ctcgaataag tcttccaaat cgttggcaac
     8761 taagctgaca gggagcgaaa ccccgtgttc tttaccttta atatcagtaa attttaataa
     8821 gacaaattta atatcattgt cttgaatcag tttagataca ttagcgattg cgtttgacat
     8881 agtaaaataa gtcctcgttg ggataaatga ataataaaaa agctcggtta tcatagcatt
     8941 ttttaaccaa gtgtgtgatg ttttttagtg tttcaaagct aaaagaagtt gcagtaatgt
     9001 tgcaaatttt tgctaaaatc ttaccgcttg catattgttt gtatggtggg gagagaaaag
     9061 cgtgtaatgt tgccgattta tcatattcta ggctccgaga ttgagcatca taaccaacag
     9121 gttttaacct tttttcagca agagcttttg ccgttattgc cagactccga acattttttt
     9181 tatgtggtag gcaagccttc actgcaaact gcttttcctc atttgcggat taagcagttc
     9241 tcttcacaaa aagcgattgc gaacgcctta attgcaaaaa aacggcaaaa tccgaccgct
     9301 tgttttctgt tacacgggca gtttaaccct tggatttggc tggcgatagc cttaggcaga
     9361 ttgccgacca ccaacctgat ttggcatatt tggggggcgg atttatatga aatggcaact
     9421 tcgtggaaat ttaagctgtt ttacccgttt cggcgtgttg cccaacaaaa aatcttacag
     9481 gtttgggcaa caagaggcga tctggatcat ttgtggcgaa atatgcgtac aagatcagaa
     9541 caggatcatt taatctattt ccccacgaaa ttaggtgatc aaataaaaat tagccccaca
     9601 caaaatgatc gcttgacgat attgctcggc aattcaggcg atccagcgaa tgagcttatt
     9661 caagccttaa gcgagattca tcaacgacta gggaacaatg tgcggattat cataccaatg
     9721 ggctacccgg aaaataatga aagctatatt cggcaggtgg aagcctatag caagcggtta
     9781 tttttggcgg aaaatttgca aattttgcgg aagaagctga gctttgaaga gtatgttacg
     9841 ttattgagcc attgtgatct cggctatttc aattttaaac gccagcaagg cattggcacg
     9901 ataagccttt tgattagcca taatgtacct tgtgtgttac attcagccaa tccgtttggg
     9961 ttggatctac aggcagaaaa agtgccttat ttgttggcgg aaaatctgac tgcttcacag
    10021 attcaaacaa ccaaacaaca actaaagagc atagataaaa accgcattgc gttttttgct
    10081 ccgggctaca ctaaactatg gcaacagcga ttaatcgaat taatgcaaaa tgaataattt
    10141 aataccactt ggcggatttt atcttgtttc tgtcgggcta tttttcagct tactttatca
    10201 actttatcag aaatcgggga tttctttcca tttactgttt agtttgctct attttgttac
    10261 tttttacgcc ggttttccgt tttcgttaat aatggcattt ggctttgatt acccgctgcc
    10321 ggagctaaaa acacaatggc aagtttttac ggcagcaggt gttggctatt tgatttatta
    10381 cgttgtctat tcacgcttgt ggagtgctgt gtcgcaacca actgctcttg ttgcaaattt
    10441 tcaggcaaaa acgaccgctt gtatccttgc cgctgtggca gttgttgccc tgctatattt
    10501 cctttattta aacggttttc tgcttttcaa actcgaaaaa tatagccaac tgtttgccaa
    10561 agatagggtc atactttcgg cattaaaacg atttttctac tttttattgc cggcgttgtt
    10621 aattttttgc ttcctatata agaataaaac agcgtggtgg gcgttactga ttgttggtgg
    10681 ggcattcggc atattaagct atgtggcagt gggcggcaca tgggcaaatt tagcactagc
    10741 ggttggcttt ttcttattga ttggattgca gcaaggctat ctgtcgttat caaaggtgat
    10801 tgccgtaagc ctaatcggcg tggtggcgat gttcttcctc gctctagccc gttacaaact
    10861 taccctttcc ggtagcgaga tgatttacac cttcttatat ttaagccgag atactttttc
    10921 gccttgggaa aatttggctc gtgtattttc taatgaggtg gaatatcaag gtttaatgcc
    10981 gattgtgcgg gatttctatg tatatatccc acaatcttta tggcaagaac gccctgagat
    11041 cgtctggaat acggcgaatt attttaccaa agtgatttta ggcaaccaat cagggttagc
    11101 tatttcgcct accttactcg gctctttcta tattatgggc ggatttagcg ttatggcgtt
    11161 gggaatgggg atagtagctg ccatgattaa agccttagat aacctattcg cttacgccaa
    11221 agcgtgcaat tccgccttgt tgcaagccta ctgcttcggc aatttattca atttgatcgt
    11281 attagtgcga gagggaatcg atgcatttgt ttcccgtttt tgctttttca cgctgatatt
    11341 tatggcttgc tatttgctgg cgaaaatcct ttcaatgggg aaaaataata atgttcgata
    11401 aagtgatcat tcggggcatt gcgttacaag cggtcaaaaa tcgggaaatt tttgcaaatt
    11461 ggctgatgaa tgaaaagcgg attcagttcg gaagattagt ggcaattaat gctgagaaag
    11521 tgattctgtc ggaaaaaaat ccgttgttac gccgagtgat agatgaagcc gagttcaact
    11581 atgcagacgg catcagtgtt gttcaatcga ttcgaaaaaa atatccaaac tataaaatag
    11641 agcgaattgc cggagccgat ttatgggaaa ccttaatgca aagagccgga gcgttaaatg
    11701 tgccggtgtt tttggtcggc agctcggcgg ataccctaac gaaggtggaa gcaaaattgg
    11761 cacaatggaa ggtgaatatt gtcggtaggc agaatggcta ttttaaggcg gagcaagaac
    11821 aggacgttat agagcaaatt aaagcaagtg gcgcgaagtt tattagtgtg gcaatgggaa
    11881 ctccaaaaca ggaacttttt atccaacagt tacaacaaca atatccacaa gccttatata
    11941 tgggtgttgg cgggacttac gatgtgtttg ccggtaaagt gaaacgagca cctaaaattt
    12001 ggcaaaattt ggggctggaa tggctttatc gactattgaa acagccaacc cgctggcaaa
    12061 gacaattaaa tttactaaaa tatgcctatt actatctgac aaatcagctc taaaaaacgc
    12121 attttttgat atttattaaa cgttttgaat aaaataaaac caaacaatac atttttttac
    12181 atttttttta aaaagtgcta gacaagggaa agtgaaatct ataatatacg cctccgttgc
    12241 gacgcattaa gttgaattga tgtaaatcga agcgttcgta gctcagttgg atagagcgtt
    12301 ggcctccgga gccaaaggtc gcaagttcga atcttgtcga gcgcgccaga tgtcaatgcg
    12361 gcactatcaa cacaatggtg gctatagctc agttggtaga gccctggatt gtgattccag
    12421 ttgtcgtggg ttcgaatccc attagccacc ccattcaata ctcttgtgag tatcagacgg
    12481 cgagtagcgc agcttggtag cgcaactggt ttgggaccag tgggtcgtag gttcaaatcc
    12541 tatctcgccg accaattta
//
LOCUS       DANLZC010000072        12473 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.072, whole genome shotgun sequence.
ACCESSION   DANLZC010000072 DANLZC010000000
VERSION     DANLZC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12473
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.072"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(313..495)
                     /locus_tag="PXE44_001532"
     CDS             complement(313..495)
                     /locus_tag="PXE44_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589033.1"
                     /translation="MYQFGYLRTKIGTDTQRAMLWNCPTYVLIIPFLLNTSVKKQYHA
                     FWQKYHQHNNDVSFPY"
     gene            645..1685
                     /gene="selD"
                     /locus_tag="PXE44_001533"
     CDS             645..1685
                     /gene="selD"
                     /locus_tag="PXE44_001533"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:690..692,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDL5589034.1"
                     /translation="MSEPIKLTQFSHGAGUGCKISPKVLGTILQTQLEQFVDPNLLVG
                     NETADDAAVYDLGNGQAIISTTDFFMPIVDAPFDFGRIAAANAISDIYAMGGKPIMAI
                     AILGFPVNKLPAEVAQQIVEGGRFACQQAGIALAGGHSIDSPDPIFGLAVTGIVPTER
                     VKRNASAVAGCELFLTKPLGIGILTTAEKKGLLKPEHQHLASEVMCQINLIGSAFSEL
                     PEVTAMTDVTGFGLLGHLSEVCQGSGLRAEVDFEDIQVLDGTKEYIALGAVPGGTNRN
                     FDSYGHLISAMTDEQKAILCDPQTSGGLLVAVLPQAVEKVQQIANRANVPLFHVGRLL
                     EMEQGKPRIEVI"
     gene            1685..2158
                     /locus_tag="PXE44_001534"
     CDS             1685..2158
                     /locus_tag="PXE44_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL5589035.1"
                     /translation="MFKPNVTMACVVHCKGKFLFVEEIEYGKRTLNQPAGHLEKDETL
                     LEGASRELFEETGIRAEMANLVKIYQWQAPRSQTDFLRFLFSVELEEWLEPNPQDADI
                     TQALWLTLAEFEDYIQQQGQCARSPLVVQSVKDYLSGERYPLSLLNAFEWARNGK"
     gene            2148..3356
                     /gene="ubiH"
                     /locus_tag="PXE44_001535"
                     /gene_synonym="visB"
     CDS             2148..3356
                     /gene="ubiH"
                     /locus_tag="PXE44_001535"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDL5589036.1"
                     /translation="MENKTTTQFDVVIVGGAVTGSVLALALSSFTHHKISIAIVEKSL
                     PDYEQQGGFDARSIALAEGSLQKMGQIRPLAGSSLGEMISEIGTPIQQIQVADKGHFG
                     KTTLKASEQNVAQLGMVVELAKFGRKLTACLAQQANITLFCPNQVVGIERSQQHCGLS
                     LKNGEKLSCSLLVAADGIQSQIAKQCGVETVKVRDYAQSAIIANVELSESHQNQAFEY
                     FTPQGPFALLPLSGNMMSLVWCVKEPNELIQLSDSEFLARLQQQFGWKLGQFVRVSKR
                     FVYPLILQKAQSHIHHRLAVVGNAAQMLHPVAGQGFNLGLRDLTTLAELVALAFNQGK
                     DIGEFSLLSTFENERTQDQARMINATSGLISLFCCEMLPVQVVRNLGLLTVSHSKIAR
                     DFVANQALGW"
     gene            3619..4851
                     /locus_tag="PXE44_001536"
     CDS             3619..4851
                     /locus_tag="PXE44_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HDL5589037.1"
                     /translation="MTTENMSTPKQAFIGLQMLFVAFGALVLVPLITGLDPNVALLTA
                     GVGTLIFQICTKRQVPVFLASSFAFIAPIQFGVQQWGIPVTMGALLCSGFIYMALSAL
                     VKAKGVNILHKYFPPIVIGPVIMVIGLGLAPVAVDMATGKSANIAYNTAIIVSMITLV
                     TTLIVAVFAKGLLKLVPILAGLTVGYILSVFMGLVDFTKVVEAPWFSLPKVSAPEFKL
                     DAILYLLPIALAPAVEHLGDVMAISQVAGKDFMKKPGLHRTLLGDGLATSAAAALGGP
                     PNTTYSEVTGAVMLTKNFNPKIMTWAACWAIAFSFCGKIGAFLATIPTFVMGGIMMLL
                     FGAVAVVGINTLIKAKVDLSIPRNLCIASVVMTFGIGGMLINIGEFSLKGISLCALVA
                     IVLNIVLPKEKVEDSAAH"
     gene            4918..5904
                     /locus_tag="PXE44_001537"
     CDS             4918..5904
                     /locus_tag="PXE44_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL5589038.1"
                     /translation="MQKLTFSPPLLNTPCPWCSELESLRELYACEYTGAVTTRTSMPQ
                     PYPHDWAKNQYVLFDSNAQKTASVNTQDATSLQTASLNTIGLSPNNLDTTISFVRTIS
                     NELTAPSSKPFIISVFGSPEEVGECYEKIVAFQTEVKMPLAMEINISCPNIPGEISPA
                     YSAEELSHYLHALQTSLKKLAQGDNTLPIGIKIPPFTYQNQYDELIKGLLQAVENGEN
                     LPISFITSTNTLGSSLVLNAGKAVLNSEVGTGVGGMAGTAIHALSLGNVYTIRQMLDQ
                     EPKLKHLQIIGVGGVNDKAGFERMKSVGADFVGLATALGVKGIGVFKEILSE"
     gene            complement(5945..6355)
                     /locus_tag="PXE44_001538"
     CDS             complement(5945..6355)
                     /locus_tag="PXE44_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HDL5589039.1"
                     /translation="MLYSVLTAHILLNEPYRNGAHSTQHEHLVVMFEIDEESGYFYAM
                     DLHQTEQPVVDSLFVFRVSDIEQNSLKEPRRLEICWSEDGYQAFLLINGYPHAVFDFR
                     QFVGYNHTKFPMPELGSMWVHKETTNELVEKWLG"
     gene            complement(6421..7731)
                     /gene="eno"
                     /locus_tag="PXE44_001539"
     CDS             complement(6421..7731)
                     /gene="eno"
                     /locus_tag="PXE44_001539"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL5589040.1"
                     /translation="MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTG
                     SREALELRDGDKGRFLGKGVLKAVSAVNNEIANALVGKDGSAQAEIDQIMIDLDGTEN
                     KSKFGANAILAVSLATAKAAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHA
                     DNNVDIQEFMIQPVGASTLKEALRIGAEVFHNLAKVLKAKGLNTAVGDEGGFAPNLAS
                     NADALACIKEAVEKAGYVLGKDVTLAMDCASSEFYDKERNVYDMKGEGKTFTSQEFTH
                     YLEGLCKEYPIVSIEDGQDESDWEGFAYQTKVLGDKVQLVGDDLFVTNTKILKNGIEK
                     GIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQ
                     IKTGSMSRSDRVAKYNQLIRIEEALERAGTPAPFNGRKEIKGQA"
     gene            complement(7922..9100)
                     /gene="rlmC"
                     /locus_tag="PXE44_001540"
     CDS             complement(7922..9100)
                     /gene="rlmC"
                     /locus_tag="PXE44_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDL5589041.1"
                     /translation="MSSLLHCSHFERGDCTSCQWIEKPYSTQLAAKEADLKRLILPFI
                     LQNKTEILPLVGSPISQFRNKAKMVVSGSVERPILGILKDQNDAQSGVDLTDCLLYPS
                     SFHRLFPILKDFIARAGLVPYNISKKKGELKYILITESQYNQSFMLRFVVRSEAKRAL
                     VERELPELLEKLPPNSVVSLNIQPQHAALLEGETEIFLTEQKVLEEHFNGIPLFIRPQ
                     GFFQTNPVVASQLYHTAQSWIEHLPIAKLWDLFCGVGGFGLHCANALQAKNSNVELTG
                     IEISASAIASATKSAEKLGLKQVKFASLDSAQFALNEAGSPPDLVIVNPPRRGIGKLL
                     AEFLNQLGSPYLIYSSCNAVSMANDFTFLTNYELHKVQLFDMFAHTAHYETLTFLVKK
                     "
     gene            complement(9093..10130)
                     /gene="nagZ"
                     /locus_tag="PXE44_001541"
     CDS             complement(9093..10130)
                     /gene="nagZ"
                     /locus_tag="PXE44_001541"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDL5589042.1"
                     /translation="MLLIDIKDKELTQEEVEMLEHPLVSGLILFTRNFYDKAQIQALV
                     KSIRQRVKKPLLITVDQEGGRVQRFREGFTKLPAMQSFNQLATTPEQAMQLAKETGWL
                     MAAEMFALDIDLSFAPVLDLGHECKAIGDRSFGQEPAAILPVAEAFIDGMREIGMATT
                     GKHFPGHGHVLADSHLETPFDDRPKERIFNHDILPFKQLIAKGKLSAIMPAHVVYTQC
                     DSQPASGSSYWLKEVLRKQLNFNGVIFSDDLGMKGAGFMGNFVERSEKAIQAGCDLLL
                     LCNEPEGLVQVLDGLKYQPTQAQKERRLSLMKRKTVTWRELENSSRYQQTQAELTALQ
                     NNWLEWKEANV"
     gene            complement(10256..10987)
                     /locus_tag="PXE44_001542"
     CDS             complement(10256..10987)
                     /locus_tag="PXE44_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDL5589043.1"
                     /translation="MFDWITNPEAWVALFTLTGLEIVLGIDNIIVISILVSRLPIEQR
                     QSARIIGLGLAMGTRILLLLSLAWMMRLVEPLFTIGNNPISGRDLILLIGGLFLIVKS
                     AMELKESISAESHEEKSESIKKASFFMVLIQIAIFDIIFSLDSVITAVAMADDIPVMV
                     IAIIIAVAVMMLAAKTIGDFVDNNPTIKNLALAFLILIGIVLVGEGFDIHIPKAAVYT
                     AMGFSVVVEMLNIKMRRNQAKLEQA"
     gene            complement(11076..11756)
                     /gene="mutH"
                     /locus_tag="PXE44_001543"
     CDS             complement(11076..11756)
                     /gene="mutH"
                     /locus_tag="PXE44_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDL5589044.1"
                     /translation="MQLSTFSPTEQALLSKAEWLAGFTLGEIAEMLHIPIPADLKRDK
                     GWVGTLIETALGAKAGSKAEQDFAHLGIELKTIPVNQKGLPLETTFVSLAPLTQNNGI
                     IWETSHVKHKLSRVLWIPVEGERKIPLAERHIGQPILWSPTITQESRLQQDWEELMEL
                     IVLGELHKINATLGEVLQLRPKGRNNRSITSAINAKGEIVQSLPLGFYLRKHFTAEIL
                     QNFLHCPL"
     gene            complement(11848..>12473)
                     /gene="lexA"
                     /locus_tag="PXE44_001544"
     CDS             complement(11848..>12473)
                     /gene="lexA"
                     /locus_tag="PXE44_001544"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDL5589045.1"
                     /translation="LTARQQEIFDFVKNHIETTGMPPTRVEIAREIGFKSPNAAEEHL
                     KALARKGYIEMLSGTSRGIRILVEDENQAANDEDEGLPLIGKVAAGTPILAVEHVENH
                     YPVNGAMFNPSADYLLKVNGNSMEKIGILDGDLLAVHKTNTARNGQVVVARVNDEVTV
                     KRLEKKGELIYLHPENDELKPIVVNPAQEYIEIEGIAVGVIRSNAWM"
BASE COUNT         3491 a         2536 c         2791 g         3655 t
ORIGIN      
        1 aggaaaaatg tgctgtggca gaacaccgat ggcaacctta cttgatggaa aacggatttg
       61 ggtggaaaag aatttaagct caaattaatc tgacagacac ggtaatttaa aacgggagac
      121 tgtcagatca agtctgatct tctacaacta agccttctca gaataaataa attttcacaa
      181 gcggtgcaac tttgcaaaaa cttcggtatt atgtagcagt tgcacaaaac cccaaattca
      241 ttaaaaccgc ttaaaaatga aatgccctaa actgattttt cagtctaggg catttattat
      301 cacatattat ggttaatagg gaaaactcac atcattgtta tgctggtgat atttttgcca
      361 aaatgcgtga tattgttttt tcaccgatgt gtttaataaa aacgggatta tcaagacata
      421 ggtcgggcaa ttccatagca ttgcacgttg tgtatccgtg ccgattttgg tgcgtaagta
      481 accaaactga tacattagac ccaatccaac cgtcacctat tctaatagca taataactaa
      541 ggtcgcatca ttaaattaaa agctcatttt ttgcaaaaaa tttcccaaat tccaccgctt
      601 gttggcgtac aataggcata attttgattt acgatggagg cagaatgtct gaaccgataa
      661 aacttacgca gttcagccac ggggcgggtt gaggctgtaa aatttcgcct aaggtgttag
      721 ggacaatttt acaaactcaa ttagaacaat ttgtcgatcc gaatttattg gtaggcaatg
      781 aaacggcgga cgatgctgcc gtctatgatt tagggaacgg gcaggccatt atcagcacca
      841 ccgatttctt tatgccgatt gtggacgccc cgtttgattt cgggcgaatt gctgctgcca
      901 atgcgattag cgatatttat gcgatgggtg gcaagccgat tatggcgatt gcgattctgg
      961 gctttccggt gaataaattg ccggctgaag tggctcaaca aattgtcgaa ggtgggcgtt
     1021 ttgcgtgcca gcaagccggt attgccttgg cgggcgggca ttcgattgat tcgcccgatc
     1081 cgattttcgg cttggctgtt accggcattg tgccgaccga acgggtgaaa cgcaatgcct
     1141 ctgcggtggc tgggtgtgag ctgtttttaa ccaagccttt ggggattggc attctaacca
     1201 cggcagagaa aaaaggtttg ttgaagcctg aacatcaaca tctggcaagc gaggtaatgt
     1261 gccaaatcaa cttgattggt tcagcgtttt ccgaactgcc tgaagtcacg gcaatgactg
     1321 atgtaaccgg ttttggctta ctcgggcatt taagcgaggt gtgtcaaggc tcgggcttac
     1381 gggcggaggt tgattttgag gatattcaag tccttgatgg cactaaggag tatattgcat
     1441 tgggagcagt tccgggtggg acaaatcgca atttcgacag ctatgggcat ttgatttcgg
     1501 caatgacgga cgagcaaaaa gcgattttgt gcgatccgca aacctctggc ggtttattag
     1561 tggcggtgct gccacaagcg gtcgaaaaag tgcaacaaat tgcaaatcgg gcaaatgtgc
     1621 cgttattcca cgttggcaga ttgctggaaa tggaacaggg caaaccacgg attgaggtaa
     1681 tttgatgttt aaaccaaatg taacaatggc ttgcgtggtg cattgtaagg gaaagtttct
     1741 gtttgtggaa gaaattgaat atggcaaacg tacccttaac cagccggcag ggcatttaga
     1801 aaaagatgaa accttgctgg agggggcaag ccgtgagctg tttgaggaaa ccggtattcg
     1861 ggcagaaatg gcaaatttgg tgaaaattta tcaatggcaa gcccctcgtt cacaaaccga
     1921 tttcttgcgt tttctgtttt cggtggaact ggaagagtgg ctggagccga atccgcaaga
     1981 tgcggatatt acccaagccc tttggcttac tttagcagaa tttgaagact acattcagca
     2041 acaagggcaa tgtgccagaa gcccgttggt ggttcaatcg gtcaaagatt atttaagcgg
     2101 tgagcgttat ccattaagtt tgttgaatgc gtttgaatgg gcgagaaatg gaaaataaaa
     2161 caacaaccca atttgatgtg gtgattgtgg gcggggcagt gaccggctcg gtgctggcat
     2221 tggcgttaag tagttttact catcacaaaa tctcgattgc gatagtggaa aaatcgctgc
     2281 cggactatga acagcaaggc ggttttgatg ctcgcagtat tgccttagcc gaaggcagtt
     2341 tgcaaaaaat ggggcaaatc agaccgcttg caggctcaag tttaggcgag atgattagcg
     2401 aaatcggcac gccgattcag caaattcaag tagcggataa agggcatttt ggtaaaacga
     2461 ccttgaaagc aagcgagcag aatgtggctc aactgggtat ggtggtggaa cttgcaaaat
     2521 tcggcagaaa attgaccgct tgtcttgcac agcaagctaa tattacgctt ttttgcccga
     2581 atcaagtggt tggcattgag cgttcccaac aacattgtgg attgagcttg aaaaatggtg
     2641 agaaactaag ctgttccttg ctggtggcgg cagatggtat tcaatctcag attgcaaaac
     2701 agtgcggtgt ggaaaccgta aaagtaaggg attatgcaca atcggcaatt atcgccaatg
     2761 tggaactgag tgaatctcac caaaatcaag cctttgaata ttttacgcct caaggccctt
     2821 ttgctttatt gcctttgagc ggaaatatga tgtcgttggt gtggtgtgtg aaagagccga
     2881 acgagttaat ccaactttca gacagtgaat ttttagcccg tttgcaacaa caatttggtt
     2941 ggaagttagg gcaatttgtt cgagtgagca agcggtttgt ttatccgctc attttgcaaa
     3001 aagcccagtc gcatattcac caccgcttag cagttgtcgg caatgccgca caaatgcttc
     3061 acccggttgc tgggcaaggt ttcaacttag gcttgcgtga tttgactacc ttagcggaat
     3121 tagtggcttt ggcgtttaat caagggaaag atataggcga attttcgctc ttatcaactt
     3181 ttgagaatga gagaacgcaa gatcaagcca gaatgataaa tgctacaagc ggtctgattt
     3241 cgcttttttg ttgcgaaatg ttaccagttc aggttgtgag gaatttaggg ttattaacgg
     3301 tttcccattc aaaaatagcc agagattttg ttgctaatca ggcattaggt tggtaaaaaa
     3361 cacaaaagca gcaattttct atttgacact gtttttaaaa aggcgtatta tccaacccgt
     3421 ttaaaaatct caccaaagcc ttgaatgcaa attcaaggct ttttcctttt tagtgaaagc
     3481 ctatgaattt tgtttgccgt aaaatcggca aaaaaatttt gcctaaaatt cgcaatcgtt
     3541 tgcgaaatag gtttatagcg ttgttttcat ctgggtaact gcaatggtta catcgcaata
     3601 catcaaagag agtaaaaaat gaccacagaa aatatgagta cgccaaagca ggcgtttatc
     3661 gggctgcaaa tgctgtttgt tgcatttggg gcgttagtat tagttccgct gattaccggt
     3721 ttagatccta atgttgccct tcttaccgcc ggtgtcggta cgttaatctt ccaaatttgt
     3781 actaaacgtc aagttcctgt tttcctcgca tcgagtttcg cttttatcgc tccaatccaa
     3841 ttcggtgttc aacaatgggg aattcccgtc acaatgggag cattgctctg ttccggtttt
     3901 atttatatgg cattatctgc tttagtgaaa gcgaaagggg tgaatattct gcataaatac
     3961 ttccctccaa tcgtgattgg accggtaatt atggtgattg gtttaggtct tgctcctgtt
     4021 gcggtggata tggcaacggg taaatcggcg aatattgcct ataacaccgc tattattgtt
     4081 tcaatgataa cgctggtaac tacgctgatt gtggcggtgt ttgcgaaagg gctattaaag
     4141 ctcgtgccaa tattagcagg tttaacggtg ggatatatct tgtctgtttt tatgggattg
     4201 gtggatttta ctaaagtggt tgaagctcct tggtttagtc taccgaaagt ttccgcaccg
     4261 gaattcaaat tagatgcaat cttgtattta ttacctatcg cacttgctcc ggcagtcgaa
     4321 cacttaggcg atgtaatggc aatcagccaa gttgccggta aagattttat gaaaaaaccg
     4381 gggttacacc gcacgctttt aggtgatggt ttagcaactt cagcagctgc ggcattaggt
     4441 ggcccaccaa atacgactta ctctgaagta accggtgcgg taatgctaac caaaaacttc
     4501 aatccgaaaa ttatgacttg ggctgcgtgc tgggctatcg ctttctcatt ctgcggaaaa
     4561 atcggggcat tccttgccac tatccctact tttgtgatgg gcggtattat gatgttgctg
     4621 ttcggtgcag ttgcggttgt ggggatcaat actctaatta aagctaaagt tgatttaagc
     4681 attccacgta atctttgtat tgcttctgta gtaatgacat tcggtattgg cggtatgtta
     4741 attaacatcg gcgaattctc attaaaaggg atcagcttgt gtgcattagt cgctattgta
     4801 cttaacattg tattaccaaa agaaaaagta gaagattctg ctgctcatta atcaaaggac
     4861 gctgtggcgt cctttttgct aataaccctc tatttgtaaa taaaaaggaa taacactatg
     4921 caaaaactca cattttcacc accactttta aatacgcctt gcccttggtg ttcggagtta
     4981 gagagcttgc gtgaacttta tgcgtgtgaa tataccggtg cggtaacaac ccgtacttca
     5041 atgcctcaac catatccaca cgattgggcg aagaaccaat atgtactctt tgattccaac
     5101 gcacagaaaa cagcaagtgt gaatactcaa gatgcaacta gcttacaaac agcaagcctt
     5161 aacaccatcg gtttaagccc aaataatctg gataccacca tcagctttgt acgcaccatt
     5221 tcaaatgagc tgacagcgcc atcaagcaaa ccatttatta tttcggtgtt tggttcaccc
     5281 gaagaggtgg gggaatgtta tgaaaaaatt gttgctttcc aaactgaagt gaaaatgcct
     5341 ttggcaatgg agattaatat ctcttgcccg aatattccgg gtgaaatttc gcctgcttat
     5401 tccgcagaag aattatcaca ttacttgcac gcactacaaa cttcgctaaa aaaactggct
     5461 caaggtgata atactttacc aatcgggatt aaaattccac cttttactta ccaaaatcaa
     5521 tatgatgaat taatcaaagg cttattacaa gcggtcgaaa atggcgaaaa tttaccaatt
     5581 tcctttatta cttcaaccaa tacgcttggc tcatcgcttg tcttaaatgc tggcaaagcg
     5641 gtattaaatt cagaagtggg aacgggcgta ggcggaatgg caggaacggc aatccatgca
     5701 ctttctttgg ggaatgttta cacgattcgc caaatgttag atcaagagcc taagctcaaa
     5761 catcttcaaa ttattggtgt gggcggcgtg aatgataaag ccggctttga gcgaatgaaa
     5821 tcggttgggg cagactttgt ggggttagct accgcattag gggtgaaagg aatcggcgtt
     5881 tttaaagaga ttctgtctga ataatgaaga ggagggcgtg cattgcacgc ccgaataaat
     5941 gaaattagcc taaccatttc tccactaatt cattagtggt ttctttatgt acccacatac
     6001 tgccaagttc cggcattggg aatttggtgt ggttgtagcc gacaaattgg cggaaatcaa
     6061 acacggcgtg cgggtagccg ttgatcagta aaaaggcttg atagccgtct tcactccagc
     6121 aaatttctaa acggcgaggt tcttttaatg aattttgctc aatatcgctg acccgaaaca
     6181 caaataaact atccactacc ggctgttcgg tttggtgtaa gtccattgca taaaagtagc
     6241 cgctttcttc atcaatttca aacatcacta ctaaatgttc gtgctgggtt gaatgagcgc
     6301 cattacggta aggttcgttc agtagaatat gagcagttaa aacagagtag agcattaaat
     6361 tatccttata agtaaaatgc caccgatttc tcagtggcat ttaaatattt aatcaggcaa
     6421 ttacgcttga cctttaatct ctttacggcc gttgaacgga gccggtgtac cagcacgctc
     6481 taacgcttct tcgatacgga ttaactggtt gtatttcgct acacggtctg aacggctcat
     6541 tgaaccggtt ttgatttgac ctgctgctgt acctactgct aaatctgcaa tggttgcatc
     6601 ttcagtttcg cctgaacggt gagagataac tgcggtgtaa cctgcatctt tcgccatttt
     6661 gattgcagct aaagtttcag ttaaagaacc gatttggttg aatttgatta agattgagtt
     6721 tgcgatacct ttctcaatac cgtttttcaa gattttggta ttggttacga ataagtcatc
     6781 accaactaat tgtactttgt cgcctaatac tttagtttgg tatgcgaaac cttcccagtc
     6841 agattcgtct tgaccatctt cgatagacac gattgggtac tctttgcata agccttctaa
     6901 gtagtgagtg aactcttgtg aagtaaaggt tttgccttcg cctttcatat cgtacacatt
     6961 gcgttctttg tcgtagaatt cagatgatgc acagtccatc gctaaagtta cgtctttacc
     7021 taacacatag cctgcttttt ctaccgcttc tttaatgcac gctaatgcgt ctgcgtttga
     7081 agccaagttt ggtgcgaaac caccttcgtc acctacagca gtatttaagc ctttcgcttt
     7141 taatactttt gcaagattgt ggaatacttc agcaccgata cgaagagctt cttttaatgt
     7201 agaagcacct accggttgaa tcataaattc ttgaatgtcc acgttgttgt cagcgtgttc
     7261 accaccattg atgatgttca tcattggtaa tggcattgag taaacacccg gtgtgccgtt
     7321 taattcagca atgtatgcgt aaagcggtaa gccttttgaa gcggctgctg cttttgctgt
     7381 tgctaaagaa accgctaaga tagcgtttgc accgaatttt gatttgtttt ctgtgccatc
     7441 taaatcgatc ataatttggt cgatttcagc ttgtgcagaa ccgtctttgc ctactaatgc
     7501 gtttgcaatt tcgttattta cggcagaaac cgcttttaat acacctttac ctaagaaacg
     7561 acctttgtcg ccatcacgta attctaatgc ttcacgtgaa ccggtagaag cacctgatgg
     7621 agcggctgct aagcctacaa aaccaccttc taagtgaact tctgcttcta cagttgggtt
     7681 accacgagag tcgatgattt cacgaccgat tactttaaca attttagcca ttgttgatgt
     7741 cctctaatat acgagttaat agaaaaaaga ataggtatat agtaaagcga ttttctgctt
     7801 ttgtcttgga aaaactgatt gagtggcaga aaatttgcca ctaatttgat ctaagactat
     7861 attcgcctga gattatagca acctttgcaa aaaacggcaa aaatttgccc gcttgtaatg
     7921 cttatttttt gactaaaaaa gtcaaggttt cataatgtgc tgtgtgagca aacatatcaa
     7981 acagctgcac tttgtgtagt tcgtaatttg tcaaaaatgt aaaatcgttc gccattgaga
     8041 cggcattaca actggagtaa atcaaataag gtgagcctaa ttgatttaaa aattctgcta
     8101 atagtttgcc gataccacgt cgaggtgggt tcacaatcac taaatcgggt ggagaaccag
     8161 cttcatttaa agcaaattgt gcagaatcta atgaggcaaa ttttacctgc tttaggccga
     8221 gtttttcagc cgatttggtc gcacttgcaa tagctgaggc tgaaatctca atgcctgtca
     8281 gctccacgtt cgagttcttc gcttgcaagg cgttggcaca atgtaagccg aaaccaccga
     8341 caccgcagaa caagtcccac aatttagcaa taggtaaatg ctcaatccag ctttgtgctg
     8401 tgtggtaaag ctggcttgct accacagggt tagtttggaa aaagccctga gggcgaataa
     8461 atagcggtat gccgttaaaa tgttcttcca gcactttttg ttcggtgagg aaaatctcag
     8521 tttcgccttc taaaagagca gcgtgttggg gctgaatatt cagactaact actgagttgg
     8581 gtggcagttt ttccaacagt tcgggcaatt cccgttcaac caatgcacgc tttgcttcac
     8641 tacgaaccac aaaacggagc ataaatgatt gattatattg gctttcggta atcagaatat
     8701 attttagctc accttttttc ttactgatgt tatacggcac taaaccagct cgagcgataa
     8761 aatctttcaa aatcggaaaa agtctatgaa atgaggacgg gtagagcaga cagtcggtta
     8821 aatccacccc gctttgagca tcattttgat cttttaaaat gcctaaaatc gggcgttcta
     8881 cactgccgga gaccaccatt ttcgctttgt tacgaaattg cgaaattggc gagccgacaa
     8941 gcggtaagat ttccgtttta ttttgcaaaa taaacggcaa aatcaaccgc ttgagatcgg
     9001 cctctttggc ggctaattgc gtgctgtatg gcttttcaat ccactggcac gaagtacagt
     9061 cgccacgctc aaaatgcgaa caatgcaaca agctagacat tcgcttcctt ccattccagc
     9121 cagttgtttt gtaaggccgt taattcagcc tgagtttgtt ggtagcgaga gctgttttcc
     9181 agctctcgcc aagtcacggt tttgcgtttc attaaagaaa ggcgacgttc tttttgtgct
     9241 tgagtcggct gatatttcag cccgtctaac acttgcacca ggccttcagg ctcgttacaa
     9301 aggagcaata aatcacagcc ggcttggatt gctttttcag aacgctcgac aaaattgccc
     9361 ataaagcccg cacctttcat tcccaaatcg tcagagaata tcacaccgtt aaagttcagc
     9421 tgtttacgca atacctcttt aagccaatag cttgaaccgc ttgccggctg gctatcacat
     9481 tgggtgtaaa cgacgtgggc aggcataatg gcagagagtt ttcctttggc aataagctgt
     9541 ttgaacggca gaatatcgtg attaaaaatc cgctctttcg ggcgatcatc aaacggggtt
     9601 tctaaatgcg aatcggctaa aacgtgtcca tgaccgggga agtgtttgcc ggtggtcgcc
     9661 ataccgattt cacgcattcc gtcaataaag gcttcggcaa caggcaaaat cgctgctggc
     9721 tcttgcccga atgagcgatc gccaatcgct ttgcattcgt gtcctaaatc cagtaccgga
     9781 gcgaaactga ggtctatatc gagggcaaac atttctgctg ccatcaacca gccggtttct
     9841 ttcgctaatt gcattgcttg ctctggcgtt gtggcaagtt ggttaaaaga ttgcattgcc
     9901 ggcagtttag taaagccttc acgaaagcgt tgcacccgac cgccttcttg gtcgacagta
     9961 ataagcaacg gttttttcac ccgctggcga atggatttca ctaatgcctg aatttgtgct
    10021 ttgtcgtaga aattacgggt gaataaaatt agcccggaaa ctaaagggtg ttccagcatt
    10081 tcgacttctt cttgagtcag ttctttatct ttaatatcaa tcagtaacat aattttcgtt
    10141 gagctatttg caaaaaaatg agcagatatg accgcttgtt gtgcctattc tacattaaaa
    10201 agcccttgat gttgatcaag ggcgaatgat taagggcata aatgagacag agcaattatg
    10261 cttgttctaa tttagcttgg ttacgacgca tttttatgtt gagcatttca accacaaccg
    10321 agaagcccat tgccgtataa accgctgctt ttggaatatg aatatcaaaa ccttcgccga
    10381 ctagtacaat accgattaaa atcaagaacg ctaaagcgag atttttaatg gttgggttgt
    10441 tatctacaaa gtccccaatg gttttcgctg ctaacatcat taccgcaact gcaatgataa
    10501 tcgcaataac cattaccgga atatcatctg ccattgccac agcagtaatg actgaatcaa
    10561 gggagaaaat aatatcaaaa attgcaattt gaattaacac cataaaaaag ctggcttttt
    10621 taatggattc gcttttttct tcgtgagatt cggcagaaat ggattctttc agctccattg
    10681 cacttttaac aatcaggaat aatccaccga tgagtagaat gagatctcta cctgaaattg
    10741 gattgttgcc aatagtgaaa agaggctcga ccaaacgcat catccaagca agcgagagaa
    10801 gcaataaaat acgggttccc attgccagcc ccaagccgat aattcgggca gattggcgtt
    10861 gttcaatagg taaacgggaa acgagaatac tgatgacgat gatgttatca atgcctaata
    10921 cgatttcaag cccggtaagt gtaaagagcg caacccaagc ttcaggattt gtgatccagt
    10981 caaacataaa aatgtccttt taaaaataag ggattaagtg atattttcta tcaaatagaa
    11041 ttaaagccga catcttatag agtgtgagga caaatttaaa gggggcaatg caaaaagttt
    11101 tgtaaaattt cggcggtaaa atgtttacgc aaatagaaac caagagggag agattgaaca
    11161 atctcccctt tagcgttgat agcagaggtg atagagcggt tatttctacc tttgggtctg
    11221 agttgtaaca cttcgcctaa ggttgcatta attttatgca gttcacctaa cacaatcaac
    11281 tccattaatt cctcccaatc ttgctgtagg cgggattctt gtgtgatagt cggagaccat
    11341 aaaatcggct gaccgatatg gcgttcggca aggggtatct ttcgttcccc ttcaaccgga
    11401 atccaaagca cacgggaaag cttatgttta acgtgggagg tttcccaaat aataccgttg
    11461 ttttgggtta aaggagcaag gctgacgaat gtggtttcca gcggtaatcc tttctgattg
    11521 accggaatgg ttttcagctc aatgcctaaa tgtgcaaaat cctgttcggc tttactgccg
    11581 gctttcgcac ctaaagcggt ttcaatcaga gtaccgaccc agcctttatc tcgtttcaga
    11641 tctgctggta tcggaatatg aagcatctcg gcaatttcac ctaaagtgaa ccctgccagc
    11701 cattccgctt tgcttaataa tgcctgctcg gttggagaga atgtagataa ttgcattgca
    11761 cttagttaat tagactaaac tggcaagcgg tcaaatttag gcagaaattt gcaaaaaatt
    11821 actaaaaaat aaccgcttgt gaggaaatta catccaagca ttactgcgaa taacgcctac
    11881 tgcaatgcct tcaatttcaa tatattcttg agcagggttt actacaatcg gttttaattc
    11941 atcgttttca gggtgtaagt aaattaattc acctttcttc tctaagcgtt taacagtcac
    12001 ttcatcattt acccgagcaa ccaccacttg cccgttacga gcggtattgg ttttatgaac
    12061 ggctaatagg tcaccgtcta aaataccgat tttttccatt gaattgccat ttactttgag
    12121 taaataatca gcacttggat taaacatcgc accattcacc gggtaatggt tttcaacgtg
    12181 ttcaaccgct aaaataggtg tgccagcagc tactttgcca atcaacggta agccttcatc
    12241 ttcatcgttg gcagcttggt tttcgtcttc gactaaaatg cgaattccac gagatgtacc
    12301 tgataacatt tcaatatagc ctttacgagc aagggctttt aagtgttctt ctgccgcatt
    12361 aggtgattta aagccgattt cacgggctat ttctactcgg gttggtggca taccggtggt
    12421 ttcaatatgg tttttgacga aatcaaaaat ttcttgctga cgggcagtta gat
//
LOCUS       DANLZC010000073        12345 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.073, whole genome shotgun sequence.
ACCESSION   DANLZC010000073 DANLZC010000000
VERSION     DANLZC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12345
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.073"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            7..588
                     /gene="nfuA"
                     /locus_tag="PXE44_001545"
     CDS             7..588
                     /gene="nfuA"
                     /locus_tag="PXE44_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDL5589046.1"
                     /translation="MHISISDAAQAHFARLLEQQEEGTNIRIFVVNPGTPGAECGVSY
                     CPPNAVEETDTEFKFNRFSAFVDDISLPFLDEAEIDYVTDPMGSQLTLKAPNAKMRKV
                     ADDAPFIERLDYVIQTQVNPQLASHGGKVTLIEITEDKYAVLQFGGGCNGCSMVDVTL
                     KEGIEKQLLAMFPDELVGVKDITEHQHGEHSYY"
     gene            complement(756..2393)
                     /gene="pyrG"
                     /locus_tag="PXE44_001546"
     CDS             complement(756..2393)
                     /gene="pyrG"
                     /locus_tag="PXE44_001546"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDL5589047.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPY
                     INVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIRSKMSKANNFTSGKIYSEVLRK
                     ERRGDYLGATIQVIPHITNEIKARVLEGGKGRDVAIVEVGGTVGDIESLPFLEALRQL
                     AVDIGREKTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPE
                     NEKKKIALFCNVPERAVISLKDVDSIYRIPELLKSQGLDSFVCDRFRLDCKEADLSEW
                     EQVLYCQANPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLTHRLTVNIKYIDSQDIE
                     TKGTELLAGLDGILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARN
                     VAGLTEANSSEFDKDCSQPVIGLITEWQDAEGNVETRSDESDLGGTMRLGSQQCHLIE
                     GSKARELYGAETIVERHRHRYEVNNKLLPQIEAAGLKVSGLSADRKLVEIIEVPNHPW
                     FIAAQFHPEFTSTPRDGHPLFAGFVKAAKDHQESRKA"
     gene            2657..3706
                     /gene="ansB"
                     /locus_tag="PXE44_001547"
     CDS             2657..3706
                     /gene="ansB"
                     /locus_tag="PXE44_001547"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDL5589048.1"
                     /translation="MKIKKLLLPTLLSIATMPIQAADLPNITILATGGTIAGSQKNTA
                     TSAYQAGQLNIETLIEAVPDIKKLANIKGEQIVKIGSQDMSDEVWLKLAKAINQQCPN
                     TDGFVITHGTDTMEETVYFLDMTVKCEKPVVLVGAMRPATEKSADGVLNLYNSVVVAK
                     DSKSSGRGVLVAMNDIVLGARDVTKTSTTSVQTFNSPNFGALGYIHNSKVDYERSPES
                     KHTVNTPFKVDELTELPKVGIIYAYSNMPTEPLQALLDASYQGIVVAGVGNGNMNAAN
                     LALLEEAAKKGITVVRSSRVPTGYTTRDAEIDDSKYGFIASGTLNPQKARVLLQLALT
                     QTNDIKTIQQYFEDF"
     gene            complement(3780..4424)
                     /locus_tag="PXE44_001548"
     CDS             complement(3780..4424)
                     /locus_tag="PXE44_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589049.1"
                     /translation="MRIWQKLASVIVVTLGLISNASAENFKLKYSSNYLMPAYINFNN
                     GGGQYNIQAKINVPLYNIVFSAKGTEKNEQFHMLSYRDTRNGKTYAMAEIDSKTVKYG
                     KVKEPLKEEALTMPTYDLFTTAFQLSYYDKLPSSFQTTNGKKLYPTPNVQLRKAQKEV
                     KVGGKTYQEITYKFRTSDKKDITVKKFEGEKFPRYIAYSRDGDNYELTFDSFVK"
     gene            complement(4425..5408)
                     /gene="cysB"
                     /locus_tag="PXE44_001549"
     CDS             complement(4425..5408)
                     /gene="cysB"
                     /locus_tag="PXE44_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDL5589050.1"
                     /translation="MKLQQLRYIVEIVNQNLNITEAAEVLYTSQPGISKQVRLLESEL
                     GINIFERNGKHVKGLTPAGEKIVAIARELLVKAQSIKSVAEEQTRPNKGILRIATTNT
                     QARYMLPSVIEKFKARYPEVSLHLHQGSPNQIYDALVAGEVDLAITTEAQYLFDEAVL
                     LPCYWWNRSVIVKPEHPLAAFVNQSKNLTVEQLGRYDLITYTFGFTGQSDLDHAFNKE
                     GILPNIVFTATDADVIKTYVRLGLGVGIMASMAHTETDKDLVAINASHLFQPSMTQIA
                     FKRGTFLRNYMYDFINYFSPHLTRVQVEKAEQLRDNNAISRLFESISLEEK"
     gene            complement(5405..6088)
                     /gene="yjjG"
                     /locus_tag="PXE44_001550"
     CDS             complement(5405..6088)
                     /gene="yjjG"
                     /locus_tag="PXE44_001550"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDL5589051.1"
                     /translation="MKYQWVLFDADETLFSFNSYLGLKAMLARYQIDFSEQDYQDFQA
                     VNKPLWVAYQNNEITAQDIQTRRFVKLSAQTGVDPLQLNQELMAEMAFVSQPLEGVMD
                     MLNALYGKVKMGIITNGFTDLQQKRLDNTQTSHFFDIVVISEQIGAAKPDRQVFDYAF
                     ALMDEFDRTKVLMVGDTLASDILGGNNAGIDTCWLNLLSKENDTDIKPTYEVHTMQQI
                     IAIVEGKSL"
     gene            complement(6164..7057)
                     /locus_tag="PXE44_001551"
     CDS             complement(6164..7057)
                     /locus_tag="PXE44_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HDL5589052.1"
                     /translation="MNKKLPPLNALKAFESAARHLNFTKAAEDLFVTQAAVSHQIKLL
                     EDFLGASLFHRRNRLLELTELGAQYFYEVQPLLEQIALATDKIRQQSSRQILTISVPQ
                     TFGMHWLVPRLADFHEKYPEVEVRIKGVDQDEGLLGKETDVAIYYGKGDWQGLESIRL
                     TESRIVILASPEYLSQNTIHLPADLAGKHLLHSSTHNKWKRMVEHLHIEDIVDAETGS
                     IFSHTFMVLQAAMHQQGIAIANQLLAQHEIEKGTLIEPFPTTLFDEKSFYVVYPSQSA
                     QVQKVQFFVEWIQQKMKECCA"
     gene            7503..8540
                     /gene="rsgA"
                     /locus_tag="PXE44_001552"
     CDS             7503..8540
                     /gene="rsgA"
                     /locus_tag="PXE44_001552"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDL5589053.1"
                     /translation="MSKPKLTHNQQRRIQSNHHKRINKKEIEWQDEMLGEMQQGIVVT
                     RHVKHADVETAEGQIFRCNIRRTLKNVVVGDQVSWRKGSEQLQGISGVIEAVFPRKNE
                     LTRPDYYDGIKVMASNIDQIIIVSAVLPTLSLNIIDRYLVICETANIPALIVLNKIDL
                     LNESERQAVKEQLKIYEEIGYETLCLSADTGENMQALHQYLAKGTSIFVGQSGVGKSS
                     LINQLLPEVNALTGSVSDNSGLGQHTTTASRLYHLPQGGQLIDSPGIREFGLWHLEPE
                     QITLGYREFQSVLGTCKFRDCKHKTDPGCALRQEVENGEISAIRFENYHRLIESLSET
                     KSQRHFRKEEV"
     gene            8537..9136
                     /gene="rnhB"
                     /locus_tag="PXE44_001553"
     CDS             8537..9136
                     /gene="rnhB"
                     /locus_tag="PXE44_001553"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL5589054.1"
                     /translation="MMNEFIYPNARLIAGVDEVGRGPLVGAVVTAAVILDPNNPIQGL
                     TDSKKLSEKKRQALAEEIKAKALSWSLGRAEPTEIDELNILNATMLAMQRAVAGLHIQ
                     PDFVLIDGNRPPKLPMPAQAVVKGDSLVAEISAASIIAKVARDQEMDELDQQFPQYGF
                     AQHKGYPTKLHFEKLAEFGATPYHRKSFAPVRKVLGLID"
     gene            9149..9508
                     /locus_tag="PXE44_001554"
     CDS             9149..9508
                     /locus_tag="PXE44_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HDL5589055.1"
                     /translation="MTTEYQKTIFTKIINKEIPANIVYQDELVTAFRDIAPQAPTHIL
                     IIPNKFIPTVNHVSNEDELALGRLFTVAAKLAQEEGIAEEGYRLVMNCNKNGGQEVFH
                     IHMHLLGGQKLGPLVAK"
     gene            9511..9882
                     /locus_tag="PXE44_001555"
     CDS             9511..9882
                     /locus_tag="PXE44_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDL5589056.1"
                     /translation="MRISYFAFAIFSIFLTACGSKPVSYLPTGSEPIVNIESDIAEKI
                     ELETNSKQFSTTNLTEFPLNLTYKLFWYNKDGVTQSHNGISDSIGWQHLWLQPKQKQT
                     IALIKPTEESHNYRLYLRGVK"
     gene            complement(9917..10546)
                     /locus_tag="PXE44_001556"
     CDS             complement(9917..10546)
                     /locus_tag="PXE44_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5589057.1"
                     /translation="MFNLQIIPVSPFQQNCTVIWDENKNAAIIDGGDNAEGIIQFVES
                     QQLKVQKLLITHGHLDHIMAVEKLREHFQVEVFGSHLADKPLFERLPEMCQQFGLPPV
                     NGFLPDHWLEEGDRVEVGELSYTVRHLPGHAPGHIGFFDFDNRIAFSGDVLFQNSIGR
                     TDLYLGNHSQLLETIREKMFDLDDDFIIIPGHGPHTTIGREKQTNPFLK"
     gene            complement(10563..11117)
                     /locus_tag="PXE44_001557"
     CDS             complement(10563..11117)
                     /locus_tag="PXE44_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDL5589058.1"
                     /translation="MKQINVQRRKWLSLGGLVLGASLLPGQVMAALSTPAPAALRFRN
                     VNTGDTYTAKFGAGGLNKTDLSQLNYLMRDRHINQVKAIDPKLFVKLNQLQRRLGFHN
                     AEILVLSGYRSAQTNARLRRRSRGVASHSYHILGQAVDFQVSGVPLYKVKQAAESLNN
                     GGVGYYPRSNFVHVDTGPVRTWRG"
     gene            complement(11287..>12345)
                     /locus_tag="PXE44_001558"
     CDS             complement(11287..>12345)
                     /locus_tag="PXE44_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HDL5589059.1"
                     /translation="LDYLYYNKNVFKNANNWLYNLGSYVPKSPSSEQIFQWTNAVREG
                     DSGQFVTDLVPRNHLYRDTAQRVLAMAGGSSAPKAKKGKAVTEVAGGGNGASFYKLAL
                     NAQRLRIIPSFNNGIFVNIPSYQLYYFRDGNLALQSKVIVGREDRRTPVMYSKLSNVV
                     VNPPWNVPTSIMTKDIIPKLARDPGYADRASFEILDSSGNKINPRSVNWSQYLNAKSS
                     PYRIRQKAGDDSALGRYKFNMPSSDAIYLHDTPNRGLFGKTGRALSSGCVRVEKSDEL
                     ATILLKEAGWGSDKKNQVLGSKKTTSAQIRSDNPVYLYYVTAWVEGGKVQTLPDIYNF
                     DAKIPQHSVNWGKLKSII"
BASE COUNT         3782 a         2625 c         2292 g         3646 t
ORIGIN      
        1 caaaccattc atatttcaat atcagatgca gcacaagccc attttgccag actattagaa
       61 cagcaagaag agggaactaa tatccgtatt tttgtggtca atcccggcac accgggggca
      121 gaatgcggtg tctcttactg cccacccaat gcagttgaag agacggatac cgaatttaaa
      181 tttaaccgtt tctctgcatt tgtcgatgat attagcttac cgtttttaga cgaagcggaa
      241 attgattatg taaccgaccc aatgggttca caacttaccc tcaaagcccc taacgccaaa
      301 atgcgtaaag tggctgatga tgcaccgttt atcgagcgtt tagattatgt gattcaaacc
      361 caagtcaatc cacaacttgc cagccacggc ggtaaagtga ccttaattga aatcaccgaa
      421 gataaatatg ccgtgctaca attcggcggt ggttgtaacg gttgctcaat ggtggatgtg
      481 accttaaaag agggaattga gaaacagctt ctggcaatgt tccctgatga attagttggt
      541 gtgaaagata ttaccgaaca ccaacacggt gaacattctt actattaaaa atactaaaag
      601 ggataaatca ttgatttatc cctttcttat aattaatgag caagcggtca aatttactcg
      661 ctttcgctcg cacttcgtgc catcagcaaa gccgatgttc aaacgcaagc gtttgtacaa
      721 aaaaatgagt aaattcgacc gcttgttaag tttgattaag ccttacgact ctcttggtga
      781 tctttcgcag ctttcacaaa acctgcaaat aatggatgac catcacgcgg agttgaggta
      841 aattccggat ggaattgtgc tgctataaac caagggtgat ttggcacttc aataatttcc
      901 actaatttac gatctgccga taaaccgctc acttttaagc ctgccgcttc aatttgtggt
      961 aacagcttat tgtttacttc gtaacggtgg cgatgacgtt caacaatcgt ttccgcacca
     1021 tataactcac gagcttttga gccttcaatt aaatggcatt gttgtgagcc taaacgcatt
     1081 gtaccaccta aatctgactc atcgctgcgg gtttccacat tcccttccgc atcttgccat
     1141 tcggtaatta agccaatcac cggctgtgag caatctttat caaattcaga agaatttgcc
     1201 tcggttaaac ctgccacatt acgggcatat tcaattaggg cgatctgcat acctaaacag
     1261 atacctaagt aaggaatctt gttttcacgg gcatattgtg cggttcgaat tttgccttcc
     1321 acgccacgat agccaaagcc gcccggcaca agaatgccgt ctaagcctgc caataactct
     1381 gtgcctttgg tttcaatatc ttgtgaatca atgtatttaa tattcactgt taaacgatgc
     1441 gttaaacctg cgtgtttcaa agcctcattc accgatttat acgcatcagg taattccaca
     1501 tattttccga ccataccgat agtcacttcg ccggttgggt ttgcttggca gtatagcact
     1561 tgttcccact ctgaaagatc ggcttctttg caatctaaac ggaaacgatc gcacacaaag
     1621 gaatctaacc cttgcgattt caataattcc ggaatgcgat aaatcgaatc tacatcttta
     1681 agcgaaataa ccgcacgctc cggcacatta cagaaaaggg cgattttttt cttctcattt
     1741 tccggaatag cacgatctga acggcaaatt aacacatccg gctgaatacc gattgaaagc
     1801 aactctttta ctgaatgctg agtcggctta gttttcactt cccctgcggt tggaatataa
     1861 ggcactaagg ttaaatgcat aaacaaggtt ttttcacgac cgatatccac cgctaattgg
     1921 cggagtgctt ctaagaatgg cagtgattca atatccccca ccgtgccacc tacttctaca
     1981 atggcgacat cacgcccttt accgccttct aacacacggg ctttaatctc gttggtaata
     2041 tgcggaataa cctgaatggt tgcacctaaa tagtcgccac ggcgttcttt acgcaacact
     2101 tcagagtaaa ttttaccgct ggtaaagttg ttggctttgc tcatttttga acggataaaa
     2161 cgctcgtagt gacctaaatc caaatcggtt tccgcaccgt cttcggtcac aaatacctcc
     2221 ccgtgctgag ttggactcat tgtacccgga tcgacattga tataaggatc aagcttcata
     2281 atggttacat ttaaaccacg agcttccaga atagatgcta aagaggctgc cgcaataccc
     2341 ttccctaaag aagatacaac accgcctgtg acgaaaatat aattcgttgt cattgtgatt
     2401 gcctttgctg attgattagg ttggaaattc agaatatcag tttacttcgt aaaaaagaaa
     2461 ctatcaggac gggaaagtag tttacatcaa attacatttt tttgctaatg gaaaatcggc
     2521 aagcagtcag attaattctt ttttcaataa tgccgactaa tcttaattaa tacactatta
     2581 tttgcgatag atcagaatta acctacatgt ttatagatat cataatacct gtttttctaa
     2641 tactggagac gatacaatga aaatcaaaaa attactactg ccaactctgt taagcattgc
     2701 tactatgcca attcaagcag ccgacctacc taatattaca attcttgcaa ctggagggac
     2761 aattgccggt agccaaaaaa atacggcaac ctcagcttat caagctgggc agctaaatat
     2821 tgaaacactc atagaagccg tcccggatat taaaaaatta gcaaatatca aaggagaaca
     2881 aatcgttaaa atcggatctc aggatatgtc tgatgaagta tggctaaaac tggcaaaagc
     2941 aattaatcaa caatgcccaa acacagatgg cttcgttatt acgcacggca cagacactat
     3001 ggaggaaacc gtatacttcc ttgatatgac tgtaaaatgc gaaaaacccg tagtattagt
     3061 gggggccatg cgaccagcaa ctgaaaaaag tgccgacggc gtattaaatt tatataactc
     3121 agttgttgtc gccaaagatt ctaaatctag tggcagaggg gtgttggttg caatgaatga
     3181 tattgtattg ggcgctcgcg atgttacaaa aaccagtact acatcagtgc aaacatttaa
     3241 ctcaccaaac tttggcgcac taggctatat ccataatagc aaagttgatt atgagcgctc
     3301 tcctgaaagt aaacacaccg taaacacccc tttcaaagta gatgaattaa cggaattgcc
     3361 aaaagtcggc attatttatg cttattcaaa tatgccaacc gaaccgcttc aagcattatt
     3421 agatgcgagt tatcaaggca tcgtggttgc aggagttggc aatggcaata tgaatgcggc
     3481 taatttggct ttacttgaag aagcagctaa aaaaggaatt accgtggttc gctcgtctcg
     3541 agtgccaacc ggatatacta cccgagatgc agaaattgat gatagcaagt atggttttat
     3601 tgcttccggc acattaaatc ctcaaaaagc tcgagtatta ctgcagctcg ccttaacaca
     3661 aacaaatgat ataaaaacta ttcaacaata ttttgaagat ttctaaaaag taaataacaa
     3721 gcggtctatt ttgcaaaaca aatcacaaaa tagaccgctt gtctcattac taatctttat
     3781 tattttacaa agctatcaaa agttagctca taattgtcgc catcacgact ataggcgata
     3841 tagcgaggga atttttcacc ttcaaatttt ttcaccgtaa tgtctttttt atcactggtt
     3901 ctaaacttgt aggtaatttc ttggtaggtt ttgccgccta cttttacctc tttttgggct
     3961 ttgcgtagct gcacattcgg tgttgggtaa agttttttac cgttagtagt ttgaaaactg
     4021 ctcggtaatt tatcgtaata gctcaactga aaggctgtgg tgaacaaatc ataagttggc
     4081 atagtcagcg cttcttcttt tagtggctct ttcactttgc catattttac cgttttagaa
     4141 tcaatttctg ccatcgcata agttttgccg ttacgggtat ctcggtagct caacatatga
     4201 aattgttcgt ttttttctgt tcccttcgca gaaaagacaa tattatataa aggcacatta
     4261 atttttgcct gaatattata ttggccacca ccgttattaa agttaatgta ggctggcatt
     4321 aaataatttg agctgtattt caacttaaaa ttctccgcac tggcgtttga aatcaagccc
     4381 aaagtgacca cgattacgct cgctaacttc tgccaaattc tcatctattt ctcctctaaa
     4441 gatatacttt cgaacaacct tgaaattgca ttgttatctc gtaattgttc cgctttctcc
     4501 acttgtaccc gtgttaaatg tggcgaaaaa taattaataa aatcatacat atagttacga
     4561 agaaaagttc ctcgtttaaa ggcgatttgc gtcatactcg gttgaaacag gtgacttgca
     4621 ttgatagcga ctaagtcttt atccgtttcc gtatgtgcca ttgatgccat aataccaacc
     4681 cctaagccta agcgtacata agttttgatt acatcagcat ctgtcgccgt aaatacgata
     4741 ttaggcaaaa taccttcctt attgaaggca tgatctaaat ccgattgccc tgtaaaacca
     4801 aaagtatagg ttatcaaatc atatctacct aattgttcga cagtcaaatt tttactttgg
     4861 ttcacaaaag ccgcaagagg atgctctggt ttgacaataa ctgaacgatt ccaccaataa
     4921 cacggcaata aaaccgcttc atcaaataaa tattgagctt ctgtggtaat ggctaaatcc
     4981 acttctccag ccaccaaagc atcataaatt tgattcgggg agccttggtg caaatgcaaa
     5041 cttacttcag gatagcgagc cttaaacttc tcaattacac tcggtagcat ataacgggct
     5101 tgggtattgg tggtcgcaat gcgtaagatg cctttgttgg gtcgagtttg ttcttccgca
     5161 accgatttaa tgctctgggc ttttaccaat aattcacgag caatcgccac aattttttca
     5221 ccagcaggcg ttagcccctt aacgtgctta ccgttacgct caaaaatatt aattcccaac
     5281 tcactttcca acaaacgaac ttgtttgcta atccccggtt gagaagtgta aagcacctct
     5341 gcggcctcgg taatattcaa gttctggttt actatttcaa cgatataacg taactgttgt
     5401 agcttcataa tgatttcccc tctacaatcg caataatctg ttgcatcgta tgcacttcgt
     5461 aagttggctt aatatccgta tcattctctt tactaagcaa atttaaccaa caagtatcaa
     5521 ttccagcatt attacctcct aaaatatcag aagcgagggt atcgcctacc ataagcactt
     5581 tagtacgatc aaattcgtcc attaatgcaa aagcgtaatc aaacacttgg cgatcaggct
     5641 ttgcggcacc aatttgttct gatatcacta caatatcaaa aaaatgtgaa gtttgagtat
     5701 tatctaaacg tttttgctgc aaatcggtaa aaccattggt gataatgccc atttttactt
     5761 taccatataa agcatttaac atatccatta caccttctaa aggctgactg acgaatgcca
     5821 tctccgccat taactcttga ttaagttgta gcggatctac gcctgtttga gcagataatt
     5881 ttacaaaacg tctggtttga atatcttgag cagtgatctc attattttgg taagccaccc
     5941 aaagtggttt atttaccgct tgaaagtctt gataatcttg ttcactaaaa tcaatttggt
     6001 aacgagccag catcgctttt aaaccaagat aagaattaaa tgaaaacaag gtttcatcag
     6061 catcaaataa tacccattga tatttcataa tattcctttt ttaacttaga taacaagcgg
     6121 tcaaattttt ctaatttttc gcataccgct gataaagcca cgcttaagca caacactctt
     6181 tcattttttg ctgaatccac tcaacaaaaa attgtacttt ctgaacttgg gctgattgcg
     6241 aaggatagac aacatagaac gatttttcat caaacaatgt ggttgggaaa ggctctatta
     6301 aagtaccttt ttcaatttcg tgctgtgcca acaactggtt ggcaatcgca atgccttgtt
     6361 ggtgcattgc agcttgaagc accataaaag tatggctaaa aattgagcca gtttccgcat
     6421 ctactatatc ttctatatgc aaatgttcaa ccattctttt ccacttatta tgggtggagg
     6481 aatgtaataa atgcttacct gctaaatccg ccggtaaatg aatggtattt tgcgataaat
     6541 actcaggcga agctaaaata acaatacggg attccgtcaa acggatagat tccagacctt
     6601 gccaatcccc cttaccataa taaatagcaa catctgtttc tttacctaat aatccctcat
     6661 cttgatcaac acccttaata cgtacttcca cttctggata tttttcgtgg aaatccgcca
     6721 aacgtgggac taaccaatgc ataccaaatg tttgtggtac gctaatagtc agaatttgac
     6781 gactactttg ctgcctaatt ttatctgttg cgagagcaat ttgttctaat aatggctgaa
     6841 cttcatagaa atattgtgcg cctaactcag ttaattccag caaacggtta cggcgatgga
     6901 acagggaagc acctaaaaaa tcttctaata gtttaatttg gtggctcact gcagcttgtg
     6961 tgacgaagag atcttctgcc gctttagtaa aatttaaatg gcgtgcagca gattcaaatg
     7021 ctttcaatgc attgagtggt ggaagttttt tgttcataat ttttcaatct aatagattac
     7081 taaacaagca actctattat tttttctgat agtttaaata aaaaaaattc gtttgatgct
     7141 tgcaaataga aataataaaa tatccgcata cttagatgga tagtttagaa tttatttcta
     7201 ttcctaaatt ttaagtatga tgttgtgttt gcatattggt ctaggaaact agactagagt
     7261 aacgaaagtt acttgtttca cttcctgtat attttgaacc gtattggttt ataaccgccc
     7321 actttgggcg gtttttttct atgtaaaaaa acagcacaaa acctagatcg cattctatat
     7381 tattcgctac ctttattcta gcctttctgc ttttttattc tttcacttta atctattata
     7441 aaaagaggtt ttatagtatg ctaaagccta tttttcattt gcaataatta ccgagagttc
     7501 ttttgagtaa accgaaatta acccataacc aacagcgtag aatccaatct aaccatcata
     7561 agcgtatcaa caaaaaagag attgaatggc aagatgaaat gttaggagaa atgcaacagg
     7621 gcatcgtcgt tactcgccac gttaaacacg ctgatgttga aaccgcagag gggcaaattt
     7681 tccgctgtaa tatccgccgt acattgaaaa atgtagtggt gggcgatcag gtttcttggc
     7741 gtaaaggtag tgagcaacta caagggatta gtggggtcat tgaagccgtc ttcccacgta
     7801 aaaatgaact tacccgcccc gattattatg acggtattaa ggtgatggca tcgaacatcg
     7861 atcaaatcat tattgtatca gctgtgctac ctacactttc gctgaatatt atcgaccgtt
     7921 atttagtgat ttgcgaaacg gcgaatattc ctgccttaat cgtgctaaac aaaattgatt
     7981 tattaaacga aagcgaacgg caagcagtta aagagcagct caaaatctat gaagagattg
     8041 gctatgaaac cctatgttta tcggctgata cgggtgaaaa tatgcaggcg ttacaccaat
     8101 atcttgctaa aggtacgtct atttttgtcg gacaatccgg cgtggggaaa tccagcctaa
     8161 ttaaccaact gttacctgaa gtcaatgctc tcactggctc agtgagcgat aactcagggt
     8221 taggtcagca caccaccact gcctctcgct tataccattt accgcaaggc ggacagctga
     8281 ttgactcgcc gggcattcga gaatttggct tatggcattt agaacctgag caaatcacct
     8341 taggctatcg agaatttcaa tcggttttag gcacgtgcaa attccgtgat tgcaaacaca
     8401 aaaccgaccc gggttgtgcc ttaagacaag aggtcgaaaa tggcgaaatt tctgcaattc
     8461 ggtttgaaaa ttaccatcgc ttaattgaaa gcctgtctga aaccaaaagc caacgccatt
     8521 tcagaaaaga agaagtataa tgaatgaatt tatttaccct aacgcccgat taatcgccgg
     8581 tgttgacgaa gtcggacgag gacctttagt cggagcggtt gtgaccgcag ccgtgatttt
     8641 agatccgaat aacccgattc agggcttaac cgattccaaa aaattatccg agaaaaaacg
     8701 ccaagctttg gcagaagaga ttaaagccaa ggctctgagc tggtcgctag gacgtgccga
     8761 gccgacagaa attgatgagc tgaatatttt aaatgcgaca atgttggcaa tgcaacgtgc
     8821 ggttgccggc ttgcatattc agccggattt tgtgctaatt gacggcaatc gtccgccaaa
     8881 actgccaatg ccggcacaag cggtggtgaa aggagacagc ttagtggctg aaatcagtgc
     8941 tgcttcgatt attgccaaag tcgcccgaga tcaagaaatg gacgaattag accaacaatt
     9001 tccgcaatac ggcttcgcac agcacaaagg ctacccgact aaactccatt ttgaaaaatt
     9061 agcagaattt ggggcaacac cttatcatcg taagagtttt gcccctgtaa gaaaagtatt
     9121 aggtttaatc gattagaagg aaaacacaat gactacagag taccaaaaaa cgattttcac
     9181 caaaatcatc aataaagaaa ttccggcaaa tattgtctat caagatgaac ttgtgaccgc
     9241 atttcgtgat attgctccac aagcacccac gcatattttg attattccga ataaatttat
     9301 tccaaccgtc aatcacgtga gtaatgaaga tgaattagcc ctagggcgtt tatttacggt
     9361 ggcggcaaaa ttagcccaag aagaaggcat tgccgaagaa ggctatcgct tagtgatgaa
     9421 ttgtaataaa aacggcgggc aggaagtgtt ccatattcat atgcacttgt taggcggaca
     9481 aaaactagga cctttagtgg caaaataact atgcgtattt cctactttgc atttgcaatt
     9541 ttttcgattt ttttgaccgc ttgtggtagc aaacctgtca gctatttacc aaccggctca
     9601 gagcctattg tcaatattga gtctgacatt gctgaaaaga tagagctgga aacgaatagc
     9661 aagcaattta gcaccacaaa tttaactgaa tttccgctta atcttactta taaattattt
     9721 tggtataata aagatggggt aacacaatcc cataacggta tatcagatag tatcggctgg
     9781 caacatctat ggttgcaacc taagcaaaaa cagaccattg cactcattaa accgacggaa
     9841 gaaagccata attaccgcct ctatttacga ggcgtaaaat agcaaaaaag cagagccaac
     9901 tgctctgctt ctttatttat ttcaaaaacg ggttggtctg tttttcccgc ccgatagttg
     9961 tgtgtggacc atgtcccggt atgatgatga aatcatcatc taaatcaaac attttttccc
    10021 tgatcgtttc caataactgg ctatggttgc ctaaatagag atctgttcga cctatgctat
    10081 tttggaataa aacatcgcca ctaaaagcaa tacgattgtc aaaatcaaaa aaaccgatat
    10141 gacccggagc atgacccggc aaatgtcgaa cggtatagct cagctcgccg acttcgaccc
    10201 gatcaccttc ctccaaccaa tgatccggca aaaagccatt tactggtggt aatccaaatt
    10261 gttggcacat ttccggcaaa cgttcaaata aaggtttatc tgcaagatgc gagccgaaaa
    10321 cctctacttg aaaatgctct ctcaactttt ccaccgccat aatatgatct aaatgaccgt
    10381 gcgtaatcaa taatttctga accttaagct gctgagattc aacaaattga ataatgcctt
    10441 cggcattatc gccgccatca ataatagccg cattcttatt ttcgtcccaa atcaccgtgc
    10501 aattttgttg aaatggacta acaggaataa tttgcaaatt aaacataaaa taagaccgct
    10561 tgttagcctc tccaagtgcg aactgggcca gtatctacgt gaacaaagtt actacgagga
    10621 taataaccta cgccaccatt attcaagctc tcagccgctt gttttacttt atataacggc
    10681 acacctgata cttggaaatc tactgcttgc ccaagaatat ggtaactatg gctggcaaca
    10741 ccacggcttc tacgacgaag tcttgcattt gtttgtgcgg agcgatagcc acttaatact
    10801 aggatttccg cattatggaa acctaaacgg cgttgtaatt gattcaattt gacaaataat
    10861 tttggatcaa ttgctttaac ttgattgata tgacgatccc gcattaaata gtttaactgg
    10921 cttaaatcgg ttttatttaa tccacctgca ccaaatttag cagtgtaagt atcgcctgta
    10981 ttcacattac gaaaacgtaa cgcagctggg gcaggagtag aaagcgctgc cattacttga
    11041 cccggcagaa ggctagcccc taaaacaagt ccgccgagtg atagccactt acggcgttga
    11101 acattaattt gtttcatttt ttcctcatag gttagctaat gatttataga aaatcactca
    11161 caaatgagcg aatttctata caccatactt aaagtaaaaa tttacaatcg taaatttatg
    11221 taagctatat tttcaaaaag ttccaagaag atgatatctt cttggaattt atcagaaata
    11281 gcacgattaa ataatgctct ttaattttcc ccaattcaca gaatgttgtg ggattttggc
    11341 atcaaagtta taaatgtccg gaagggtttg cacttttcca ccttcaaccc aagccgtcac
    11401 ataatagaga taaaccgggt tatctgaacg aatctgagcc gaggtcgtct tcttactgcc
    11461 taagacttga ttctttttat cagatcccca acccgcttct tttaataaaa tggttgccaa
    11521 ttcatcagat ttttctacac ggacacaacc agaacttaac gcacgccccg ttttaccaaa
    11581 taatcctcta tttggcgtat catgcaaata aattgcatcc gaacttggca tattaaattt
    11641 ataacgtcct aaagcgctat catcacctgc tttctggcga atacggtatg gtgaactttt
    11701 agcatttaaa tattgtgacc aatttacgct acgcggatta attttattgc cactactatc
    11761 aagaatttcg aaactagcac gatctgcata acctgggtca cgcgctagtt taggaataat
    11821 atctttagtc ataatactag tcggtacatt ccaaggtgga ttcactacca cattgctcag
    11881 cttactatac attaccggtg tacggcgatc ttctcgccca acaatcactt tagattgaag
    11941 agctaaatta ccatcacgga aataatataa ttgatagctt gggatattca caaaaattcc
    12001 attgttaaaa cttggaataa tacgtaaacg ttgagcattg agcgctaatt tatagaaaga
    12061 agccccatta ccgccaccag ctacctccgt aacagcttta ccttttttgg ctttaggtgc
    12121 tgaagagcct cctgccattg ccaatactct ctgtgccgta tcacgatata aatggtttct
    12181 tggaacaagg tctgttacaa actgcccaga atcgccttcc ctcacggcat tagtccattg
    12241 gaagatttgc tctgaagagg gcgattttgg tacataactg cctaagttat ataaccaatt
    12301 atttgcattt ttaaacacat ttttgttgta atacaaataa tctaa
//
LOCUS       DANLZC010000074        12290 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.074, whole genome shotgun sequence.
ACCESSION   DANLZC010000074 DANLZC010000000
VERSION     DANLZC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12290
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.074"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(183..1316)
                     /locus_tag="PXE44_001559"
     CDS             complement(183..1316)
                     /locus_tag="PXE44_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL5589060.1"
                     /translation="MKTIKIGLVGTGYIGRCHAIAYAQAPTLFPLKGKIQLEYLAEIN
                     QALADQKAREFGFQRATDDWRKLVTDPNVDVVDICTPNFLHKEIALEAIKHGKHVYSE
                     KPLALTAQDAKEMVIAAQQAGVKTLVGFNYIKNPTTQLARQIIQNGEIGEVVHFYGTH
                     NEDYLANPNTPIDWHCYKAKAGLGTLGDLAAHIVSIAHYLVGSDIERVIGDMQTVIEE
                     RPSPHNPAEKIKVENEDQASSLIRFANGVMGTLESSRISCGRKMGLTYVVTGTKGTLS
                     YTQERMAELKLYLHDDDKSRQGFKTILVGPEHPDYAAFCVSAGHGIGFNDQKAVEIRD
                     LINGIAADDPMYPDFEEGYKISRVLEAIAKSAEEKRWVEVSEI"
     gene            complement(1447..2475)
                     /locus_tag="PXE44_001560"
     CDS             complement(1447..2475)
                     /locus_tag="PXE44_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5589061.1"
                     /translation="MTTQFLKNMGMVDDKGKIDFIAFFERFGVLIFLILLIIFFTSQN
                     GAFLSQRNIYNVLTEVSIFGIMAVGMTFVILTAGIDLSVGSILAVTAMFAAYIIKGDN
                     TVTLEAGAWGGMSWLIGLSICLGLGTFIGWLHGLGVTKLKLPPFIITLGGMTIWRGLT
                     LVLNNGSPIAGFDEGYRWWGRGDILGVPVPVVIFAVVAIIGYFALHKTRWGRYVYSVG
                     GNPEAARLAGVNINRTLVSVYVVIGALAGLAGFILSARLGSAESVAGVSFELRVIASV
                     VIGGTSLMGGYGRVTGTIIGSIIMGVLINGLVLMDVSAYYQQIIMGLIIILAVAFDTY
                     AKSRRGAL"
     gene            complement(2487..4031)
                     /locus_tag="PXE44_001561"
     CDS             complement(2487..4031)
                     /locus_tag="PXE44_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5589062.1"
                     /translation="MSTPLLEVKDLTKSFSGVMALNGVKLTVGKGEVHALLGENGAGK
                     STLLKALSGAQPQTSGEIIFNGETLSLSDSPFDRQLKGIVTIYQEFNLLPNMTVAENF
                     FLGREPVKGLFVNEKAVNEEAQLVLDNLGLNIKPDTQVSRLSVAQQQMVEIARAMTLN
                     AKLIIMDEPSAALSDKEVETLHQIVRDLKSRGISVIYVTHRLNEVFALCDRFTVFQDG
                     RYSGEGEVKNVNVDQIIKMMVGREVAFTRRPPEETHHTHRPIRLAVKNLHREKPAFDP
                     HGIALHGVSFNIHEGEVLGIAGLVGAGRTEIARCLFGVENYTADEISLDGKPYAATSP
                     LDALEKGVALVPEDRKKEGLVLGLPIKTNMTLPILSKLLKGNLFVDNQKEDDLIETYR
                     QALRIKMASSELEARKLSGGNQQKVILARCMALNPKVLIVDEPTRGIDVGAKSEVHQV
                     LFDMAKQGVAVLVISSDLPEIMAISDRIITLSEGRVTGEIHGDDATEEKLMSMMAVGV
                     QRKAAA"
     gene            complement(4082..5020)
                     /locus_tag="PXE44_001562"
     CDS             complement(4082..5020)
                     /locus_tag="PXE44_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL5589063.1"
                     /translation="MKKRIKFVLSALTLGLAMNSFAKDELVVFSLPNLSSPFEVQLQK
                     VAVETSKKLEIKLQVLDGQSSSTKQASDLENAITRGAKGIIISPNDVNAISGAVEEII
                     KEKIPAATLDRKVESSKPVPHFGANNYTGGQEVAKAVKAKYPNGAKIILLTGQPGSTS
                     NIERTKGIRDELAAGGDKYKIVVDQTGNWLRSEGLRIIESVLPTLKEKPEVIISANDD
                     MALGAIEALRSQGLKAGDILVTGFDSTPEALARVKDGWLYLTADQRPSFAVSTALEQV
                     VGNIRESKEVSGADYPPKIILKDNLQEAERFAEISE"
     gene            complement(5352..6863)
                     /locus_tag="PXE44_001563"
     CDS             complement(5352..6863)
                     /locus_tag="PXE44_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL5589064.1"
                     /translation="MSQFVQNFINGAVVESQSQRVTSVFNPATGEETKKVKLSLPNEV
                     DAAIAAADQAFKSWSQQSPLRRARILFKFKELLEANFDELARLISSEHGKIYSDAIGE
                     LTRGLEVVEFATGIPHLQKGEFSANAGRGIDIHSIQQPLGVVAGITPFNFPAMVPMWM
                     FPVALACGNTFVLKPSEKDPSVSIRLAELLKEAGLPDGVFNVVQGDKEAVDILLTDSR
                     IQAVSFVGSTAIAQYIYEKGSANGKRVQALGGAKNHALIMPDADVQGTINALLGAAFG
                     AAGERCMALSVAVVVGDELADKIIEELIPKVKALKIGSGLLPQGVLENDMGPVISKEH
                     KAKIESYIDQGVAQGAKLCVDGRGYKVEGFENGYFVGGTLFDNVTPEMTIWQDEIFGP
                     VLSVVRVKDYAEGIALINSHQYGNGSAIFTADGDSARQFTQDVQAGMVGVNIPIPVPM
                     AFHCFGGWKASIFGPLNVYGTDGVRFYTRMKTITTRWPDSSVRSQAAFNFPTL"
     gene            7082..7906
                     /gene="iolB"
                     /locus_tag="PXE44_001564"
     CDS             7082..7906
                     /gene="iolB"
                     /locus_tag="PXE44_001564"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL5589065.1"
                     /translation="MSSPLLSKPNFNQHEGLIQHITPENAQWEYVGFDVYQLSKQQIT
                     LESGENELCLVFVTGFGSVYVDDYAFENIGNRTTPFEKIPPYAVYIPPHKSVLVKTEL
                     NLELAVCKAPSQGNYPVRLISPNHIGVEKRGFGNNQRLVHNILPETEAADSLLVVEVF
                     TDEGNTSSYPSHKHDDKHSTEETYLEETYYHRFDPPQGFAMQRVYTDDRTLDECMAVY
                     DKNVVKVPRGYHPVATIAGYNNYYLNVMAGPVRKWKFTWEQDHLWVNSETYKAKFE"
     gene            complement(7982..9433)
                     /locus_tag="PXE44_001565"
     CDS             complement(7982..9433)
                     /locus_tag="PXE44_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL5589066.1"
                     /translation="MPNHKFLLLRFQAYINGKFVSSQNGKTFTVQNPTNGEKIGEVAD
                     LGTEETAQAIEAAAVAQKKWQALLPKERSVILRKWFELIMQNQEELAQILSLEQGKPL
                     AESRGEIAYGASFIEWFAEESKRIYGDVLPQDKAGRRLFVTKQPIGVVAAITPWNFPN
                     AMITRKAAPALAAGCAIVIKPAAETPLSALALAQLADQAGIPKGVFNIITSSDVEGVG
                     RELSTNPTVRKLTFTGSTRVGKILMQQSAGTVKKLALELGGNAPAIVFDDADLDTAVE
                     GVFASKFRNGGQTCVCANRIYVQAGIYEQFLAKLSEKVAQIKLGSPFEEEVTIGALIS
                     ERAVEKVESHINDALSLGAELVLGGERHALGGTFFQPTILKNVTQAMKVAREETFAPL
                     APIFKFETEEEVIQWANDTEFGLAAYLFSQNIGRIWRVSEQLEYGIVGINEGIISNEI
                     APFGGWKESGLGREGSKYGIEEFLEIKYLCMKV"
     gene            complement(9611..10921)
                     /locus_tag="PXE44_001566"
     CDS             complement(9611..10921)
                     /locus_tag="PXE44_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5589067.1"
                     /translation="MIYAEIFVVLAAIYLGLRSGGLGIGLYGGLGLAILTLGFGLPMG
                     SIPVDVMLIIMTVVIGAAVLQAVGGMDLLVRYAEVLMRKNPKYINFIAPMITWLMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRALAGSVVSSQLAIAGSPLSAAMAAMIAVMEN
                     NSVTFFQVIAVCLPVSFIASMVAAFVASKQGCELQNDVVYLERLKAGIVSPSRKLASE
                     STYSKGAKLSIGIFLLATVLVVLLAAVPGLKPVYENGKSMSTRDIIIVMMLASSCLMM
                     LVGKIKPDTIVASPIFRSGMTSIAVIIGIVTLGMTFVDAHLIEIKASIGNILTEYPML
                     LAVVLFFTCALLYSQGSTSALIIPLAVSLGIPNWAILASFIAITGIFILPTYPTSLAA
                     MELDTTGSTRAGKYIIDHPFMLPALVGVAVGLAFGFVWAPMIVS"
     gene            complement(10937..11632)
                     /locus_tag="PXE44_001567"
     CDS             complement(10937..11632)
                     /locus_tag="PXE44_001567"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDL5589068.1"
                     /translation="MARNIIGILGGMGPAATADMFQKFIRLTPADCDQAHIPLLISSI
                     PDIPDRTRYILANGENPAPVMEKYIEGLESAGATCIIIACNTAHYWFEHLKAKSQVEM
                     LSMIDCVVDEVLKCGKWRVGLLATDATLAVDLYKKQLEAQGLTFICPTENGQKAVMDS
                     IYLLKSGELEKATELMLQQRDELIRLGAEVIILGCTEVPIILAQEIEREPMRYVDSTL
                     VLVKKAIDWYQTH"
     gene            11848..>12290
                     /locus_tag="PXE44_001568"
     CDS             11848..>12290
                     /locus_tag="PXE44_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5589069.1"
                     /translation="MKNIETKWLEDFLTLEECRHFSQAAEKRNLSQSAFSRRILALEE
                     VVGVKLFDRTSIPLQLTEQGKLFHSQTRNLLQQLQNNLDELLGHNCNLPNIKFAAAHS
                     LSLSIMPKLIKKLSQTNENFIYSVEAIDVDQTVNTLIEGKSDFIFS"
BASE COUNT         3566 a         2833 c         2394 g         3497 t
ORIGIN      
        1 aagtaagcat ccaaataaac aactatcaat atgaagccta aaaaccattc tgtttgcata
       61 agaacattga tagggctgtt ttttgtgcta aaaatttctt taattgaact cataattttt
      121 ccttaaatgt aaaaaaacgt acctcaacca atttgagata cgtttctatt ttaatgtaat
      181 tattaaatct cactcacctc cacccacctt ttctcctccg ccgattttgc tatcgcttcc
      241 aatactctgg agattttata gccttcttca aaatcgggat acatcgggtc atcagcggcg
      301 atgccgttga tgaggtcacg gatttccacc gctttttggt cgttaaaacc gatgccgtga
      361 ccggcactca cacaaaaagc agcgtagtcg ggatgttccg ggccgactaa aatggttttg
      421 aatccttggc gggatttatc gtcatcgtgc aaatagagtt tgagttctgc cattcgttct
      481 tgggtatagc tgagcgtgcc tttagtacct gtaacgacat aggtcagccc cattttacgc
      541 ccgcaggaaa tgcgggagct ttcgagcgtg cccattaccc cattagcaaa tcgtatgaga
      601 ctacttgcct gatcttcgtt ttctactttg attttttctg ctggattgtg cggactaggg
      661 cgttcttcaa tcactgtttg catatcgccg ataacacgct caatgtctga accaaccaaa
      721 tagtgtgcga tactgacaat atgtgcggct aaatctccca acgtaccaag cccggctttg
      781 gctttgtagc agtgccaatc aatcggggta ttagggttgg cgagataatc ttcgttatgc
      841 gtgccgtaga aatgcaccac ctcaccaatt tcgccgtttt gaataatttg gcgagccagt
      901 tgggtggttg gatttttgat atagttgaaa ccgaccaagg ttttaactcc cgcttgttga
      961 gcggcaatca ccatttcttt ggcatcttgg gcggtgagtg caagcggttt ttctgaatag
     1021 acgtgtttgc cgtgtttaat cgcttctaag gcaatttctt tgtgcagaaa atttggtgta
     1081 caaatgtcca ccacatcgac attcggatcg gtaaccaatt ttcgccaatc gtcagtagca
     1141 cgctgaaagc caaattctcg ggctttttga tcggctaatg cttggttgat ttcggcaagg
     1201 tattccagtt ggattttgcc tttaagtgga aacagggttg gggcttgagc gtaggcaatg
     1261 gcgtggcagc gaccgatata gccggtgcca actaagccga ttttgatggt tttcataata
     1321 ctatcccagt agggtgcgtt tgctaacgca ccattagaat tttcagtgca ttggcaaacg
     1381 caccctataa gaaagcggtc tggttttgca aaatttttac aaaatcagac cgcttgtgtt
     1441 agctatctat aatgctcctc gtctgctctt cgcataggtg tcgaaggcaa cggcaaggat
     1501 gatgattaag cccataatga tttgttggta gtaagcggaa acgtccatta gtactaaacc
     1561 gttaatcaat acacccataa taatggagcc gatgatggtg cctgtgactc gtccgtatcc
     1621 ccccattagg gaagttccgc cgataaccac tgaggcaatt acacgtagct caaaagaaac
     1681 gccggcaacg gattcggcac tgcctaaacg ggcggagaga atgaaaccgg caagtcctgc
     1741 caatgctccg atcaccacat aaacactgac taaggtacgg ttaatattca caccggctaa
     1801 acgggctgct tccggattgc cacctaccga gtacacatag cgtccccagc gggttttatg
     1861 caatgcgaag taaccgataa tggcgactac ggcaaaaatc actaccggca ccggtacgcc
     1921 taaaatatcg ccacgtcccc accagcggta accttcgtca aatccggcaa ttggcgagcc
     1981 gttatttaac acaagtgtta aaccacgcca aattgtcata ccgccaagcg tgataataaa
     2041 cggtggcagt ttgagtttag tgacgcctaa gccgtgtaac caaccaatga aagtgcctaa
     2101 acctaaacag atactcaaac ctatcagcca gctcattccg ccccaagcac cggcttctag
     2161 cgttaccgta ttatcgcctt tgataatata ggccgcgaac attgcggtaa cagcaaggat
     2221 tgagccgaca gagagatcga tccctgcagt taagatgacg aaagtcattc ctaccgccat
     2281 aatgccgaaa atcgacactt cggttaaaac gttatagatg ttgcgttgcg ataaaaatgc
     2341 cccgttttgt gaagtgaaga aaataatcag taagatcaag aaaatcagta cgccgaaacg
     2401 ttcaaagaag gcgataaaat cgatttttcc tttgtcatcg accataccca tattttttag
     2461 aaattgcgtt gtcatacaaa cctctcttat gctgccgctt tacgctgaac gccaacagcc
     2521 atcattgaca ttaatttttc ttcggtggca tcatcgccgt gaatttcgcc agttacccga
     2581 ccttcggaaa gagtgataat gcggtcggaa atcgccataa tttccggtaa atcggaggaa
     2641 atcactaata ccgcaacacc ttgttttgcc atatcaaaca gcacttggtg aacctcagat
     2701 tttgccccaa catcaatacc tcgtgtcggc tcatcaacaa ttagtacttt cgggttgagt
     2761 gccatacaac gggctaaaat cactttttgc tgattaccac ccgataattt gcgggcttca
     2821 agttcgctac ttgccatttt gatgcgtaac gcttggcggt aggtttcgat taaatcatct
     2881 tctttttgat tatcgacaaa caagttaccc ttcaataatt tgcttaaaat cggcagggtc
     2941 atattggttt taatcggtaa accgagaact aagccctctt ttttgcgatc ttccggcaca
     3001 agggcaaccc ctttttccaa agcatctaac ggcgaggtgg cagcataagg cttaccgtct
     3061 aggctgattt catcagccgt ataattttct accccaaaca agcaacgggc aatttcagtt
     3121 cgccctgcgc caactaagcc ggcgatccct aatacttcac cctcgtgaat gttaaaagag
     3181 acaccgtgga gagcaatgcc gtgtggatcg aatgccggtt tttcccgatg cagatttttc
     3241 acagctaaac gtattggtcg gtgggtatgg tgagtctctt ctggtggacg gcgagtaaag
     3301 gcaacttcac gtcctaccat cattttaata atttgatcga cattcacatt tttgacttca
     3361 ccctcaccag aatagcgccc gtcttggaaa acggtgaaac ggtcgcatag ggcaaacact
     3421 tcgtttaagc ggtgggtcac ataaatcacg ctaatgccac ggctttttaa atcacgcacg
     3481 atttggtgta gtgtttccac ttctttatca cttaaggcag cggacggttc gtccataata
     3541 atcaacttag cgtttaaggt cattgcacgg gcaatctcga ccatctgctg ttgtgccaca
     3601 cttaaacggg aaacttgggt atcaggtttg atatttaagc cgagattatc cagcactaat
     3661 tgtgcttcct cattcaccgc tttttcattg acgaataaac ctttcaccgg ctctctgccc
     3721 aaaaagaaat tttcagcaac cgtcatattc ggtaacaagt taaattcttg ataaatcgtt
     3781 acgatgcctt tgagttggcg atcaaacggg gaatcgctca aacttaaggt ttcaccgtta
     3841 aagataattt cgccacttgt ttgtggctga gcgcctgaaa gtgcttttaa taaggtcgat
     3901 tttcctgcac cgttttcacc caacaatgcg tgaacttcgc cttttcccac agtcagtttc
     3961 acgccattga gtgccataac accagagaaa ctttttgtca gatctttcac ttcaagtaag
     4021 ggagtagaca tacaacctcc gtaaaaaaac gccctccggc ctcagccgga aggcagataa
     4081 attattcgct gatttctgca aaacgttctg cttcttgcag attatctttc aggataattt
     4141 ttggcggata gtccgcaccg ctcacttctt tgctctcacg gatattgcca accacttgct
     4201 ctaatgcggt gcttaccgca aaacttgggc gttggtcagc ggtgagatac aaccagccat
     4261 ctttcacacg tgcaagagcc tcaggggttg agtcaaagcc ggttactaaa atatcgccgg
     4321 ctttcaaacc ttggctacgc aatgcttcaa tcgcaccaag tgccatatcg tcattcgctg
     4381 aaataatcac ttctggtttc tcttttaagg ttggtaacac actttcaata atgcgtaatc
     4441 cctcagaacg gagccagtta ccggtttgat caaccacgat tttatatttg tcaccaccgg
     4501 cagctaattc atcacgaata cctttggtac gttcaatgtt agaggttgaa cccggctggc
     4561 cggttaaaag aataattttt gccccattcg ggtatttggc tttcactgct tttgcgactt
     4621 cttgaccgcc tgtgtagtta tttgccccaa agtgtggaac cggcttactg ctttcaactt
     4681 ttcggtctaa tgtcgcagca ggaatttttt ctttgataat ttcttccact gcacctgaaa
     4741 tcgcattcac atcattcggg gaaatgataa tgcctttagc accacgggta attgcatttt
     4801 ctaagtcaga agcctgctta gtagaagaac tttgcccgtc taatacttgc aatttgatct
     4861 ccaacttttt actggtttct accgctactt tttgtagttg aacttcaaac gggctggata
     4921 ggttcggtaa gctaaatacc acaagctcat ctttcgcaaa gctgttcatt gctaagccta
     4981 acgttaaagc actcaataca aatttaatac gctttttcat tacaaaatcc tctttttaag
     5041 atgggaattg acggcgaata ttgccaatgt tacttcaaac tgcgaaggaa acttaagtct
     5101 tatttcaata ataaacctca atgaaataaa tttttcaatt tagaatatat cattcaaaat
     5161 tttccaaacc aaatgctttg tttcaaattt gttacgtaga tcacattttt aggaagagat
     5221 aaatgcaagc ggtcaaaaca ggtcgctttt ttgcaaaaat ttgaataaaa atgaccgctt
     5281 gtgagatgaa aaccaataaa aaagcggtga tttctcaccg ctaaacactc aaaaaagaaa
     5341 tgtactcgtt attataaggt tgggaaatta aatgccgctt ggctacgcac cgagctgtcc
     5401 ggccaacggg ttgtgatggt tttcatacgg gtgtagaaac gcacgccgtc tgtgccgtac
     5461 acattgagag gcccgaaaat agaggctttc caaccgccaa agcagtggaa cgccattggc
     5521 acaggaattg gaatattcac gcccaccata ccggcttgta catcttgggt gaattgacgt
     5581 gctgaatcgc catctgcggt gaaaattgca ctaccattgc cgtattggtg actgttaatg
     5641 agtgcgatcc cttcggcata atctttcaca cgcacaacag agagaaccgg tccgaagatt
     5701 tcatcttgcc agatggtcat ttcaggtgtc acgttatcaa acagcgtacc tccgacaaag
     5761 taaccgtttt caaacccttc gactttatag cctcgaccat cgacacagag tttcgccccc
     5821 tgtgccaccc cttgatcgat atagctctca attttggctt tatgctcttt ggaaatcacc
     5881 ggccccatat cattttcaag cacgccttgt ggtagtaagc ctgaaccgat tttcaatgct
     5941 ttgaccttag gaattagctc ttcaatgatt ttatcggcta attcatcacc aacaactacc
     6001 gctaccgata gtgccataca acgttcaccg gctgcaccaa atgctgcgcc cagtaaggca
     6061 ttaattgtgc cttgtacatc agcatccggc ataatcaaag cgtggttttt cgcaccgccc
     6121 aaggcttgaa cccgtttgcc gtttgctgag cctttctcat aaatatattg tgcaattgca
     6181 gttgagccga caaaactcac cgcctgaatg cgagagtcgg ttaaaagaat atctaccgct
     6241 tctttatcac cttgcaccac attgaataca ccgtccggca agccggcttc ttttaataat
     6301 tctgccaagc gaatagacac acttgggtct ttttcagacg gttttaaaac gaaagtgtta
     6361 ccgcaagcaa gtgccactgg gaacatccac atcggtacca ttgccgggaa attgaacggg
     6421 gtaatgcctg ccactacgcc caacggttgc tggattgagt gaatatcaat gccacgccct
     6481 gcattggcgg agaactcacc tttttgtaag tgtggaatac cagtggcaaa ttccaccact
     6541 tctaaaccac gggttaattc gccaatcgca tcggaataga ttttgccgtg ttctgagctg
     6601 attaaacgag ccaactcatc aaagtttgct tccaataact ctttgaattt aaataaaatt
     6661 ctggcacggc gtagtggtga ttgttgtgac cagcttttga aagcctgatc ggccgctgca
     6721 attgccgcat cgacttcgtt tggcaggctt aatttgactt ttttggtctc ttcaccggtt
     6781 gcgggattaa ataccgaggt aacacgctgg ctttggcttt caacgactgc accgttaata
     6841 aaattttgga caaattggct cattggaggc tccttatcaa ctgattgaga ggaaaaacgg
     6901 ataaaataca gccgataaac taccgctaaa aatggaaaat atcaatacat aatactaaaa
     6961 aatgaaaatt ttatttcatt tttgtgatct aagtagcaag tttacagatt gccggtatta
     7021 aaatgagaca aaatttcgta tagtttgaaa gcgtattcca tttcgttaag cgaggtttcc
     7081 tatgtctagt ccattattgt ctaaacccaa tttcaaccaa catgaagggt taattcagca
     7141 cattacccct gaaaatgcac agtgggaata tgttggattt gatgtttatc agctcagtaa
     7201 gcaacaaatt acgctggaaa gtggtgaaaa tgaactttgt ctagtctttg tgaccggttt
     7261 tggctctgtc tatgttgatg attatgcttt tgaaaatatc ggcaaccgta ccacgccatt
     7321 tgaaaaaatt ccgccttatg cggtctatat cccgccacac aaaagcgtgt tggttaaaac
     7381 cgaactcaat ttagaattgg cagtgtgcaa agcaccaagt cagggtaatt atccggtgcg
     7441 tttgattagc ccgaaccata tcggtgtgga aaaacggggc tttggcaaca atcaacgctt
     7501 ggtgcataat attctgccgg aaaccgaagc agccgacagt ttgctggtgg tggaagtctt
     7561 taccgatgaa ggcaatacca gttcctaccc aagccacaag cacgatgaca aacactctac
     7621 cgaagaaacc tatttggaag agacctacta ccaccgcttc gatccaccac aaggttttgc
     7681 aatgcaacgg gtttataccg atgatcgcac acttgatgaa tgtatggcgg tgtatgataa
     7741 aaatgtggtt aaagtgcctc gtggctacca ccctgttgct accattgccg gctataacaa
     7801 ctactaccta aatgttatgg cagggccggt acgcaaatgg aaatttactt gggaacaaga
     7861 tcatctttgg gtgaacagtg aaacctataa agccaaattt gagtaatgca agcggtcttt
     7921 ttccaccaat tttttacaaa ataagggatt aaaaatggga cgcttttggc gtcccattcg
     7981 gttatacttt catacaaaga tattttattt ctaaaaactc ttcaataccg tacttcgaac
     8041 cttcccgccc aagtccagac tctttccagc cgccgaacgg agcgatttcg ttactgatga
     8101 tcccctcatt aatcccgaca atgccatatt ccaactgctc cgatactcgc caaattcgcc
     8161 cgatattttg gctgaataag taagccgcca aaccaaactc agtatcattc gcccactgga
     8221 taacctcttc ttcggtttca aatttgaaaa tcggggcaag gggagcaaag gtttcctcac
     8281 gagccacttt catcgcttga gtgacatttt tcagaatggt tggctggaag aatgtgccac
     8341 ccagtgcgtg gcgttcaccg cctaacacca gttctgcccc aagtgaaagg gcatcgttaa
     8401 tgtggctttc gactttttcc accgctcgtt cactaattag tgcacctatc gtgacctctt
     8461 cctcaaatgg cgagccaagc ttgatttgag ccactttctc actcaatttt gcaagaaatt
     8521 gctcataaat gcccgcttgc acataaattc ggttagcaca gacgcaggtt tgcccaccgt
     8581 tacggaattt agaggcaaac acgccctcaa ccgctgtatc tagatccgca tcatcaaaca
     8641 caatcgccgg tgcattaccg cctagctcca atgccaattt ttttaccgtc cctgccgatt
     8701 gttgcattaa aattttgccg acacgggtgg agccggtaaa ggtgagtttg cgtacggtcg
     8761 gattagtgct caactcccgc cctacacctt cgacatcgct actggtaata atgttaaata
     8821 cccctttggg aatacctgct tgatccgcta attgggctaa tgctaaggca gaaagcgggg
     8881 tttctgccgc cggtttaatc acaatcgcac aacctgccgc taacgccggt gccgctttac
     8941 gggtaatcat tgcatttggg aaattccacg gtgtaattgc tgccaccacg ccaatgggtt
     9001 gtttagtcac aaacaagcgg cgaccggctt tatcttgtgg caagacatcg ccataaatcc
     9061 gcttgctttc ttcggcaaac cactcgataa aactggctcc ataggcaatc tcgcctcgac
     9121 tttctgccaa tggtttaccc tgttctagac ttaaaatctg ggcgagttct tcttggtttt
     9181 gcataatcag ctcaaaccat ttacgcaaaa tcacggaacg ctctttgggc aataatgcct
     9241 gccatttttt ctgggcaact gcggctgctt caatcgcttg agcggtttct tccgtgccta
     9301 aatcagccac ctcaccgatt ttctcaccat tagtcggatt ctgtaccgta aaggttttcc
     9361 cattttggct tgaaacaaat ttaccgttaa tataggcttg gaaacggagt agcaaaaatt
     9421 tgtgatttgg cataatcact ccttgaatgg ttaaaaagag aacaatattg actataatcc
     9481 aatttcagta aagagaaaag agctgaaaaa taacttttta ctcaataata aagcacctta
     9541 gacaagcgtt cttctaaggt gcagccattt attattgaga attaacttaa attctctaca
     9601 tatttaataa ttaagataca atcattggag cccatacgaa tccaaaagca agcccaacag
     9661 caaccccaac taaagctgga agcataaacg gatgatctat aatatatttg ccagcacgag
     9721 ttgaacccgt agtatctaat tccatcgctg ctaatgaagt tgggtaggtt ggcaggataa
     9781 aaatgcctgt aattgcaatg aaagaagcca aaattgccca gttagggatc cccaagctta
     9841 ctgctaatgg aataataaga gcagaagttg acccttgtga ataaagtaat gcacaagtaa
     9901 aaaagagtac tacagctaac aacattggat attcagttaa aatatttcca atagaggctt
     9961 taatttctat taaatgtgca tcgacaaaag tcatacctaa tgtaacaatc ccgataatta
    10021 ccgcaataga agtcattcct gagcggaaaa ttggactagc cacaatcgta tcaggtttaa
    10081 tttttcctac caacatcatt aagcacgaag aggctaacat catcacaata attatatctc
    10141 gggtactcat tgacttccca ttttcatata ctggctttaa ccctggaacg gctgcaagta
    10201 gaacaaccaa tacagttgct agtaagaaaa tgccaatcga tagttttgct ccttttgaat
    10261 aggtagattc actcgctaac ttacgagatg ggctgacgat tcctgctttt aaacgttcta
    10321 aataaacaac atcattttgc aattcacagc cctgcttaga agcaacaaat gcagcaacca
    10381 tagaggcaat aaaagaaaca ggtaagcata ctgctattac ttgaaaaaat gtaacactgt
    10441 tgttttccat tactgctatc atagccgcca ttgcagcact caatggagaa cctgcaatag
    10501 ctaattgaga agacacaacc gagccggcta atgctcttga agggcgaata ccactctctt
    10561 ttgccacttc tgcaatcaca ggtaaggtgg aaaagacgat aaaacctgta ccagccataa
    10621 tcgtcattaa ccaagtaatc atcggtgcaa taaagttaat atatttagga tttttacgca
    10681 tcaatacttc cgcataacga acaagtaaat ccatacctcc tactgcttgt aacacagcag
    10741 cacctattac aacagtcata ataatcagca ttacatcaac tggaatagaa cccataggta
    10801 aaccaaaacc tagagttaga atcgcaagcc ctaaaccacc atatagtcct attcctaagc
    10861 ccccagaacg cagtcctaaa tagattgcag caagtaccac aaagatttct gcataaatca
    10921 tatccatctc cttaaattaa tgagtttgat accaatcaat tgctttttta accaatacca
    10981 acgttgaatc tacataacgc attggctcac gctctatttc ttgagctaaa ataataggta
    11041 cttctgtaca acctaaaata attacttctg cacctaaacg aatgagttca tcacgttgtt
    11101 gtaacattag ctctgtggct ttctctaact cacctgattt taataaatag atactatcca
    11161 ttactgcctt ttgaccattt tctgtcgggc aaataaaagt cagtccttgt gcttcaagct
    11221 gttttttgta taggtctacc gccaaagttg catcagttgc caacaatcca acccgccatt
    11281 ttccacactt caaaacctca tcaaccacac aatcaatcat acttagcatc tctacctgac
    11341 ttttggcctt caagtgttca aaccaataat gtgcagtatt acatgcaata atgatgcagg
    11401 ttgcccctgc actctcaagc ccttcaatat atttttccat taccggagcc ggattctctc
    11461 catttgctaa aatatagcga gttctatctg gtatgtcggg aatagaggaa atcaataatg
    11521 gaatatgagc ttgatcacaa tcagctggtg ttaagcggat aaatttttgg aacatatctg
    11581 ctgttgctgc aggtcccatt ccacctaaaa tgccaataat atttcttgcc atacacttct
    11641 ccttataaga tttaatttac tgcgatgact tatacaaaca agttatgcaa aaaagaaatg
    11701 caataaatcg aatcagtatg caaaaaatgc aacaaagctc tttatagata gaaaaaaaat
    11761 ctttaaaatc aataaaatac taatatatca aatataaatt taatgcattt aatgaaatca
    11821 taatgcgaaa tttgcattgg ttaggatatg aaaaacatag aaacaaaatg gttagaagat
    11881 ttcttaacgc tagaagaatg tcgccatttt tctcaggcag cagaaaaaag aaatttgtca
    11941 caatctgcat ttagcagacg tattttagct ttagaagaag ttgtgggagt gaagttattt
    12001 gatcgtacct ctattccatt acaactcaca gaacaaggta agctatttca ttcacaaacg
    12061 cgaaacttat tacaacaatt acagaataat ctagatgaat tattaggaca taattgtaac
    12121 ttacctaata ttaaatttgc ggcggctcat tccctctctc tttctattat gcccaaatta
    12181 attaagaaat tatcacaaac aaatgaaaat tttatttatt ccgttgaagc aatcgatgtt
    12241 gaccaaacag taaatactct aattgaagga aaaagtgatt ttattttttc
//
LOCUS       DANLZC010000075        12237 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.075, whole genome shotgun sequence.
ACCESSION   DANLZC010000075 DANLZC010000000
VERSION     DANLZC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.075"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            134..1216
                     /gene="serC"
                     /locus_tag="PXE44_001569"
     CDS             134..1216
                     /gene="serC"
                     /locus_tag="PXE44_001569"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDL5589070.1"
                     /translation="MTEIYNFSAGPAMMPKKVLEKAQQELLNWLDQGTSVMEVSHRGK
                     LFMELAAQSEADLRKLYNVPDNYRILFLQGGARGQFAAIPMNLIGKKGKALYLNSGHW
                     SATAAKEARNFAEIDEINILETEPQLKVSRLDFSDIAEQYDYIHYCTNETISGVEIFD
                     IPNVGNTPLVADMSSNILARGIDINKFGVIYAGAQKNLGPAGITIVIIRDDLIGNARK
                     DTPSIWNYATQRDADSMINTPPTFAWYLCSLVFQHLLAEGGLKATEERNLAKATLLYE
                     YLDSSTFYYNTVAHENRSLMNVTFTTGNDELNAKFVAEATACGLQALKGHKVLGGMRA
                     SIYNAMPIDGVKALIEFMKVFEQRNA"
     gene            complement(1273..2250)
                     /locus_tag="PXE44_001570"
     CDS             complement(1273..2250)
                     /locus_tag="PXE44_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDL5589071.1"
                     /translation="MTLAVAAIIGGLIILVWSADRFVEGAASTARHFGMTPLLIGIVV
                     IGFGTSAPEMLVSASSALSGSPGIALGNAYGSNITNIALILGLTAIIKPLSVNSDVLK
                     KELPILLAVTLLSAYLVYDANVTQLDAIILLAVFFVYMAWTIITAMKSKNDALSLDVD
                     AELTNSAKMGLGKSILWLIIGLVSLVISSQFLVWGAVEVAKFFGVSDLVIGLTIVAVG
                     TSLPELASSIAAARKGEVDLALGNIIGSNLFNTLAVVGIAGAIEPMQVSAEVFSRDVL
                     MMSALTFLLFIFGINLYRRTEGGRINRLEGLLLFAAYVGYNFYLFKTAG"
     gene            2362..4929
                     /gene="glnD"
                     /locus_tag="PXE44_001571"
     CDS             2362..4929
                     /gene="glnD"
                     /locus_tag="PXE44_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDL5589072.1"
                     /translation="MMKNSNELKTLLSAFTEQQRQAFQSENVLSLLAQRSQFYDQLLQ
                     ALWQDFGLAQYEHITLLAVGGYGREEMFPLSDLDILVLTEKPLEEAIQQRLNELFALL
                     WDSKLQLGTSVRTLEECIQIGKAEISVATNMLEGRFLLGNKSLWLRLKEALYQPDFWD
                     IKAFFRAKMAEKNERYARYHNTSYNLEPDLKHSPGGLRDLHLISWIMLRHYGVSSFVD
                     LLNKGLLYPEEYKELIAAQAVLFRMRFALHLQLKRYDNRLRFDRQLQLSEILGYQGEA
                     NQAVELMMRDYFQATKSISQLSQLLLNSFEQAYVNELQKNAQKQPLDQYFYRQEQQLF
                     VVERKIFKTQPTTMLDLFFHLTNDPTLIANADTLRQLRLCLQQQHEPMCFLPEMREKF
                     VQLFAQPKVISRAIVPMHQLGFLSAYLPQWKGIEGLMQFDLFHIYTVDEHTVRVMLNL
                     ENFSEDPSRAFFPLCHQLFATLSEKPLLYLAALFHDIGKGREGEHAQVGALDMREFAE
                     LHGFSQAQINDMVWLVAEHLTMSITAQRRDIHDPQIISEFAKKVQNQTALSSLLCLTV
                     ADICATSESLWNDWKASLFTQLYQFTLQQLAQNLNYQAVAQEHRLQALELMKFVLSAD
                     ERIALNAFWQPCPERYFLHHTPTQLVWHALHYVKTRRLPMVLISNEYARGGTEIFVCC
                     QDQPQLFARIAQLLSQKRISIHDAQIITTENGVVLDSFIVSERDGDDDLTDERCMQIQ
                     QAMMKMLSLTETGIKFTKKPVKHLSFKRKTRLRFLPPSSKQQTEFELFTLDREGLLAQ
                     IGYIFNRLRLNLINAKITTIGERVEDFFVVSNDQGKALSEQEQLELKAILITELDSE"
     gene            complement(5106..5864)
                     /locus_tag="PXE44_001572"
     CDS             complement(5106..5864)
                     /locus_tag="PXE44_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5589073.1"
                     /translation="MITVKNLHHKIGNATILNNINLTIPNGGITALIGANGAGKSTLL
                     SLIARLQPIQQGEIHLNELNIANTASQTIARQLAILTQDNVIHSRITVKDLLLFGRYP
                     HHQGRITSHDNAIVEEALVRFDLSTFSHRFLNELSGGQRQRALIAMTFCQQTDHILLD
                     EPLNNLDMFHARELMKLLRKLTDELALTTIMVVHDINIAAAYADTIIAMKDGGIKMIG
                     TPKEIITTENLKTVFNLDAEVLECQGKPLVIHHI"
     gene            complement(5884..6760)
                     /locus_tag="PXE44_001573"
                     /pseudo
     CDS             complement(5884..6760)
                     /locus_tag="PXE44_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
     gene            complement(6813..7781)
                     /locus_tag="PXE44_001574"
     CDS             complement(6813..7781)
                     /locus_tag="PXE44_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL5589074.1"
                     /translation="MFSFFRLNLFLLILLIPISASVGVADFHWSEIFHSSEQSQIFFI
                     SRLPRTLAVILVGATLAVAGMVLQIVLKNRFIEPSMIGTSQSAAIGILLASLLFPTSA
                     LLLKMSIATLSALMGMGLFMLLIRQLPPHQQLMLPLIGIVFGNVIEAISTFIAYETDS
                     LQVLSIWFSGDFSGILAGRYELLWITAILAVVVYVMADQLTIAGLGENISTNLGINYK
                     QMTWLALIVVAMITAIVVVTVGQIPFIGLVVPNIVSRIAGDRLRKNLPAVVVLGANLV
                     LICDILGRAINAPYEVPISTIFGIIGTVIFLYLLLKEKRAEEKARG"
     gene            complement(7875..8774)
                     /locus_tag="PXE44_001575"
     CDS             complement(7875..8774)
                     /locus_tag="PXE44_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5589075.1"
                     /translation="MIKKALFALTATAVFAASAFAKDVTIPTARGEVTFKETPSKIAV
                     FDTGSLDTLQALGVKIDGMPEFKVLSYLKPSADKAVNVGTVFEPDLEALNSLNPDLII
                     VGTRTAKKFDEVSQIAKTIDMTDNGDKLIESGLQRLDSFGKLFNKEAETEKLKAEINT
                     LLDQTKAAVKGKGNGLIILVNGGKISAFGSGYRLSWIHDNLGIPMADSSIKNTGHGQP
                     VSFEFIQKLNPDWLFVLDRIAAIGQEGQSAQQVLDNELVRQTKAWKNGNIVYLSAASY
                     LAPGGYEQMKTDLTNIKTAFEKK"
     gene            8948..10411
                     /locus_tag="PXE44_001576"
     CDS             8948..10411
                     /locus_tag="PXE44_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL5589076.1"
                     /translation="MAEYSGTTQEKMKDKQVEQINISPHSIEAEQAVLGGIMLSNDHW
                     DNVAERVQPHDFYNYAHRIIFEQMAELVRHNKPIDIITLDQALKDKGVLQDVGGFAYL
                     AELSKNTPSAANILAYADIVRDRSDLRSVISAGNQIAEMGYKTKGRNSKEVLDEAERI
                     VFEIAEKRNNANEGPQRIDDILLKTLARMDTLSKNRHNGGVTGVSTGFVDLNKKTAGL
                     QPSDLIIVAARPSMGKTTFAMNLCENASLVDIEDKDDLDEQGNPKKKPAKPVLIFSLE
                     MPADQIMMRMLASLSRVDQTKIRTGQISDDEDTAKISSTMAILQERNNIYIDDSSGLT
                     PTELRSRARRIYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISRSLKALAKELEV
                     PVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSDLKGIAE
                     IIIGKQRNGPIGRVRLTFQGQYSRFDNYTGGHYDDEY"
     gene            10401..10706
                     /locus_tag="PXE44_001577"
     CDS             10401..10706
                     /locus_tag="PXE44_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDL5589077.1"
                     /translation="MNTKPTQPITLCAIYRSKAKEGMYLYVPKRDQFEQVPETLRQLF
                     GKPEFVMMFNLTGEKPLVRAKNEEVLQLLIEQGYYLQMPPPPENLHKAFIAQQKGEK"
     gene            10706..11155
                     /gene="nrdR"
                     /locus_tag="PXE44_001578"
     CDS             10706..11155
                     /gene="nrdR"
                     /locus_tag="PXE44_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDL5589078.1"
                     /translation="MRCPFCSTEETKVIDSRLAASGYQIRRRRECISCKERFTTFESA
                     ELVVPYVIKNNGSREPFDAEKLRTGLRRALNKRPVSADDVEKAISQIIIQLQAMGERE
                     VPSRHIGTLAMNALKQLDKVAYIRFASIYLSFSDIEEFIKEIEKLKE"
     gene            complement(11229..11411)
                     /locus_tag="PXE44_001579"
     CDS             complement(11229..11411)
                     /locus_tag="PXE44_001579"
                     /inference="COORDINATES: protein motif:HMM:NF015431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5589079.1"
                     /translation="MALLADSTVDRLVAENGLVEVLGETENPKFDLTMIYAAHRYPSS
                     IVRAFVGFVLEQVKAN"
     gene            complement(11380..12090)
                     /locus_tag="PXE44_001580"
     CDS             complement(11380..12090)
                     /locus_tag="PXE44_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5589080.1"
                     /translation="MNLNTLRLFVAVIQHGSLSKASERLNVPIATISRQIADLEKELN
                     IQLFDRQKSGVKPTMAGQRLYEQVHLSIEQLATAKEVLFEEQGNLKGKLRLSASPACE
                     PVLAWIADFQAAYPQVQVHCTLTDRLLDLSADGIDVAFRIGQLHGEQFIAKKVGQIGS
                     KWVAHPKFLARFGTPSTVKDLKNFPIAAWAKNEASVITLGTGRQAVEIPYLFSSNDSY
                     AIEYMAKSSGSACRQHGG"
     gene            complement(12087..>12237)
                     /locus_tag="PXE44_001581"
     CDS             complement(12087..>12237)
                     /locus_tag="PXE44_001581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589081.1"
                     /translation="TVLGEKHAYKESKFLQELSGVYYLYGKNQQVQGSIKHFIQEIEH
                     WGEKQ"
BASE COUNT         3916 a         2419 c         2503 g         3399 t
ORIGIN      
        1 tacgaaagag tattatgctt ttaaaaagag tattttatgc aagtttgcta aaataatttt
       61 tctgttttta ttctaccatt acgctttttt tcagatagaa tagaatcaat attattacta
      121 tacgaggtga aaaatgacag agatatataa cttcagtgcc ggcccggcaa tgatgccgaa
      181 aaaagtactc gaaaaagcgc aacaagaatt attaaattgg ctagaccaag gcacatcagt
      241 gatggaagtc agccaccgtg gaaagctatt tatggagctg gcggcacaat ctgaagcaga
      301 tttacgcaag ctgtataatg tgccggacaa ttatcgtatt ctatttcttc aaggtggtgc
      361 aagggggcaa tttgcagcca ttccaatgaa tttaatcggt aaaaaaggca aagcgcttta
      421 ccttaattca gggcattggt cggcaacagc agcaaaagaa gcacgtaact ttgcggaaat
      481 tgatgaaatc aatattttag agactgaacc gcaacttaaa gtgagtcgtt tagatttcag
      541 cgatattgca gagcaatatg attatatcca ttattgcacc aatgaaacca tcagtggggt
      601 agaaattttc gatattccga atgtcggaaa taccccgtta gtggcagata tgtcgtctaa
      661 tatcttagcc cgagggattg atatcaacaa atttggagta atttatgccg gagcgcaaaa
      721 aaatctaggg cctgctggca ttacgattgt gattattcgt gatgatttaa ttggcaatgc
      781 acgcaaagat accccatcta tttggaatta tgcaactcag cgagatgctg attcgatgat
      841 taataccccg ccaacttttg cttggtattt atgttcttta gtgttccaac atttactggc
      901 agagggtggt ttgaaagcta ctgaagaacg taatcttgct aaagctacat tgctttatga
      961 gtatttggat agttcaacat tctattacaa tacggtagca cacgaaaacc gttccttaat
     1021 gaatgtaacc tttaccacgg gtaatgacga gctaaatgca aaatttgtgg cagaagcgac
     1081 cgcttgtggt ttacaggcat tgaaaggcca taaagtatta ggtggaatgc gtgcctcaat
     1141 ttataacgca atgccgattg atggggtaaa agcactcatt gaatttatga aggtatttga
     1201 acaacgtaat gcttaattca agcttaagaa tataaattaa agagccacgt ttgtggctct
     1261 ttgaatttgg cactagcccg cagttttaaa caaatagaaa ttatagccta cataagcggc
     1321 aaagagtaat aagccctcta accgattaat tctgccacct tctgtccgac gatagaggtt
     1381 gatcccgaaa atgaataata agaaggtgag agccgacatc atcaagacat cgcgagaaaa
     1441 cacttccgct gaaacttgca ttggttctat agcaccggca atccccacca cggctaacgt
     1501 attaaataag ttagaaccga taatgttacc taaagctaaa tccacttctc ctttacgagc
     1561 agctgcaata gaagaggcta attcaggtag tgaggtgcct accgccacaa tagtaagccc
     1621 gattaccaaa tcactcacac caaaaaattt agccacttca actgctcccc agactaagaa
     1681 ttgggaacta attactagcg atactaaacc aataattagc caaagaatgg attttcctaa
     1741 tcccatctta gcactattgg ttaattctgc atcaacatct aagcttaatg catcattttt
     1801 acttttcatt gcggtaataa ttgtccacgc catatataca aaaaacacag caagtaaaat
     1861 aatcgcatca agttgagtga cgtttgcatc ataaaccaaa taagcagaaa gcagggtgac
     1921 agccagtaaa atcggcaact ctttttttaa cacatcggaa ttaactgata gtggtttaat
     1981 tattgcggtt aaacctaaaa tcagcgcaat attggtaatg ttagaaccgt aggcattacc
     2041 gagagcgata cccggcgatc cgcttaatgc agaagatgca gatacgagca tctccggtgc
     2101 agatgtacca aaaccaatga ccacaatacc gattaacaat ggtgtcatac caaaatggcg
     2161 agccgtagag gctgcccctt ccacaaaacg atccgcactc caaactaata taattaagcc
     2221 accgataata gcggcaacag caagtgtcat aaactatcct tttagatgaa aaacataaag
     2281 gcattataag cattttagcc aaaataaata taggcaaacg aaaataaatg acctattatt
     2341 ttgccagttt aggaagagat tatgatgaaa aacagcaacg aattaaaaac gcttttatca
     2401 gcatttacgg agcaacagcg gcaagcattt caatccgaaa atgtactcag cttacttgca
     2461 caacgtagcc aattttatga ccaactactg caagcacttt ggcaagattt tggactcgct
     2521 caatatgaac atattaccct attagcggta ggggggtacg gacgtgagga gatgtttccg
     2581 ctttcggatc tggatatctt agtgttaact gaaaaaccgc tagaagaggc tattcagcag
     2641 cgtttaaacg aactatttgc cttgctttgg gacagcaaac tgcaacttgg caccagtgtg
     2701 cgtacgctcg aagaatgtat tcagattggg aaagcagaaa tttcggttgc cactaatatg
     2761 ttggaagggc gatttttatt gggtaataaa tcgctttggc taagattaaa agaagcactt
     2821 tatcagccgg atttctggga tattaaagcg ttttttagag cgaaaatggc ggaaaaaaat
     2881 gagcgttatg cacgttatca taatactagc tataaccttg agccagatct caaacatagc
     2941 ccgggaggat tgcgagatct gcatttaatc tcttggatta tgctacggca ttatggtgta
     3001 tcttcttttg tcgatctcct caataaaggg ctactttacc cggaagaata taaggagctg
     3061 atcgcagcac aagcggtact ttttcggatg cgttttgcac ttcatttgca actgaaacgt
     3121 tacgataatc gccttcggtt tgacagacaa ttacaactga gtgaaatatt aggctaccaa
     3181 ggcgaagcaa atcaagcggt ggaattaatg atgcgagatt attttcaagc cacaaaatct
     3241 atttcgcaac tgagccaatt attgttaaac agttttgaac aagcctatgt aaacgagttg
     3301 caaaaaaatg cacaaaaaca accgcttgat cagtattttt atcggcaaga acagcaattg
     3361 tttgtggtcg aacgcaaaat atttaaaacc caacccacaa caatgttgga tctctttttt
     3421 catcttacta atgaccctac gttaatagcg aatgccgaca ccttacgtca gttgagactg
     3481 tgtttacagc aacaacacga gcctatgtgc tttttgcctg aaatgcgaga gaaattcgta
     3541 cagctttttg ctcaaccgaa agtcataagc cgagcaatag ttccaatgca ccaactgggt
     3601 tttttaagtg cttatctacc gcaatggaag gggatcgaag ggctaatgca attcgaccta
     3661 ttccatattt atacagtcga tgagcatacg gtcagagtca tgttaaattt agaaaatttt
     3721 agcgaagatc caagtcgggc gtttttccca ctatgtcatc agctatttgc aacgctttct
     3781 gagaaaccgt tgctctatct tgcggctctt ttccacgata ttggcaaggg tagagagggc
     3841 gaacacgctc aagttggtgc gctggatatg cgagaatttg cggaactaca cggtttttct
     3901 caagcacaaa tcaatgatat ggtttggctg gtggcagagc atttgacaat gtcgatcacc
     3961 gcacaaagac gagatatcca cgatccgcaa atcatcagcg aatttgcaaa aaaagtgcaa
     4021 aatcagaccg ctttatcttc actactttgt ttaacggttg cagatatttg tgcaaccagt
     4081 gagagcctct ggaatgattg gaaagcctcg ctgtttaccc aattatatca atttacgcta
     4141 cagcaactgg ctcaaaattt gaattatcaa gcagtggcac aagagcatcg cttgcaagca
     4201 ttagaactga tgaagttcgt gctttcagct gatgagcgaa tagcattaaa cgcattttgg
     4261 caaccttgcc cggagcgtta ttttcttcac catacaccga ctcagttggt atggcacgct
     4321 ctgcattatg taaaaactcg ccggttgccg atggttttaa tcagtaatga atatgcccgt
     4381 ggtggtacag aaatttttgt atgttgccaa gatcaacctc aactgtttgc tcgaatcgcc
     4441 caattactca gccaaaaacg cattagcatt cacgatgcac aaattattac tactgaaaat
     4501 ggtgtagtgt tagacagctt tattgtaagt gagcgtgatg gtgatgatga tttaaccgat
     4561 gagagatgta tgcaaattca gcaggcgatg atgaaaatgt taagcttaac tgaaacaggc
     4621 ataaaattta ccaaaaaacc ggtgaaacat ttgtcattta agcgtaaaac tcggctgcgt
     4681 tttcttcctc cgtcatcaaa gcaacaaacc gaatttgaac tttttacctt ggatagagaa
     4741 gggttgttgg cacaaatagg ctatattttt aaccggctcc gattaaattt aattaatgcg
     4801 aaaattacga ctattggtga aagggtagag gatttctttg tggtcagtaa tgatcaagga
     4861 aaagctttat cggaacaaga acagttagaa ctaaaagcca ttctaattac cgaactagat
     4921 agtgaatgaa aacaaccgct tggagtgaag actgcaagcg gtaaattttt caacgtttta
     4981 ggtaaattaa atcacgagtt aagagaggtt gagaattcaa gaaattagaa actaaatata
     5041 agtttctatc gtttagatta ataaattaag acgcaatttt tattaattac catgatcttc
     5101 aaacgttaaa tatgatgaat aactaaaggt ttgccttggc attctaacac ttcggcatca
     5161 agattaaaca ctgtttttaa attttcagtg gtgataatct cttttggggt gccgatcatc
     5221 ttaattccac catctttcat tgcaataata gtatctgcgt aagcagcagc aatgttgatg
     5281 tcgtgtacca ccattatagt agttaaggcg agttcatcgg ttaattttcg taataatttc
     5341 attaattccc gagcatggaa catatctaaa ttattcagtg gttcatctaa taagatatga
     5401 tcggtttgct gacagaaggt cattgcaatt aatgcccgct ggcgttgccc tccggataat
     5461 tcatttaaga atcggtggga gaaagtcgat aaatcaaatc gtaccagagc ctcttctaca
     5521 atggcattat cgtgcgaagt gattctgcct tgatgatgag ggtaacgccc aaataacagc
     5581 agatctttga cggtaatacg gctatgaatc acattatctt gggtcaagat agccagttgt
     5641 cgagcgatag tttgagaagc tgtgttggct atattcagct catttaagtg gatttcaccc
     5701 tgttggatag gttgcaagcg tgcaattaaa gagagcaggg tggatttgcc cgcaccattt
     5761 gctccgatta aagcggtaat accaccattt ggaatggtta aattgatatt attcagaatc
     5821 gtggcattcc cgattttgtg gtgaagattt ttaaccgtta tcattttctt ccttaattta
     5881 tggttatttt ttccgtttta aaatgagata aataaagaca ataccgccaa taaattcaat
     5941 aacgacactc agaacccctt gcattttcag cacttgctcg aagattgcct gccctaaaat
     6001 taaggtgatg gcagagatta aaaaagtcat tggtattcgc acgctatggc aaacagtcgg
     6061 gctaattgca ttaacaatgg cacaaacaag tagccctaga aatagaatcg ggccaacaag
     6121 tgcggttgaa acagacacca acaatccaca acagattagt aattgacgtg aaaattgatg
     6181 atattctaac ccaagcccaa ttgccttatc tttgccaagt aacaaaatat ctaatttatg
     6241 ccgttgtaac cagacccaaa tggcagagag aatagcgata cagcctccaa tccaaagtaa
     6301 tgtgggggta gtcaggttaa atgaggcaaa gaagagcctt gagcaacggc aaactctgtt
     6361 gggtcgacat tcgttgcaac aaattattta ggcttctaaa taagacacca aagatgacac
     6421 cgattaaaag cattcgggtt aaatcggatc gatttcgatt cagtgaacta aatagtaata
     6481 ttgatgcccc tagcattaac aaggtttcaa agaaaaactt atagcttggg tctaactgag
     6541 taaagcctaa accgccaaat aaaaatacta acgaggtttg caatagcaag tagagcgaat
     6601 cgaaccccaa aatacttgga gttaaaatag gattatgtgt gagtgtttgg aatagcaggg
     6661 tcgaaatacc aatcgtataa gcaatagtac agagcaaaat aagctttttg ccacgcagtt
     6721 gcaatataaa gtcccaatta cctttcgcat tataggtcat ataaaacaga atacttgcta
     6781 ataagattaa gcttaaaacg ataaagcgtg atctaaccac gagccttctc ctcagcccgt
     6841 ttctctttga gcaataaata gaggaaaatc actgtcccaa taatcccaaa aatagtagaa
     6901 atcggcactt cataaggggc attgatggct cgccctaaaa tgtcacaaat caggactaaa
     6961 ttcgcaccta acaccacgac cgcaggcaga tttttacgca atcggtcacc tgctatgcga
     7021 gagacaatat tagggacgac caagccgata aacggaatct gtccaaccgt caccacgaca
     7081 atagcggtaa tcatcgccac cacaattaag gcaagccaag tcatttgttt gtagttaatg
     7141 cctaagttgg tactaatatt ttcaccaagg cctgcaatgg ttaattgatc tgccatcaca
     7201 taaaccacaa cagccagaat agcggtaatc cagagcagtt cataccgacc tgccaaaata
     7261 ccggagaagt cgccggaaaa ccaaatcgaa agcacttgta ggctgtcagt ttcataagca
     7321 ataaaagtgc tgatagcttc aataacattg ccaaaaacga tcccgattaa tgggagcatt
     7381 aattgttgat gaggcggtaa ttgacgaatg agcaacataa aaagccccat tcccattaat
     7441 gcacttaatg tggcaataga catttttaac agtaatgctg atgttgggaa aagtaaacta
     7501 gcaagtaaaa taccaattgc cgcactttgg ctagtgccaa tcatactagg ctcaataaag
     7561 cggttcttca gcacaatttg taataccata cctgcaacgg ctaaggttgc accgactaat
     7621 atcactgcga gcgttcttgg taaacggctg ataaaaaaga tctgactttg ttcggaagaa
     7681 tgaaaaattt cactccaatg aaaatctgcc acacccacac tggcactgat tggaatgagc
     7741 aatattaaaa gaaatagatt taatctaaaa aaagagaaca tagtgataaa tagcaagcgg
     7801 tcatattctc tctctttttt acaaatgcag gcacttgcaa aaataggagg aaatctgacc
     7861 gcttgtaagg ttaattattt tttctcaaat gctgttttaa tgttagttag gtcggttttc
     7921 atctgctcat aaccacccgg cgctaaatat gaggcagcac ttagataaac gatattaccg
     7981 tttttccaag ctttagtttg acgaactaat tcattatcca acacttgttg ggctgattga
     8041 ccttcttgtc cgatagccgc aatacggtct aacacaaaca accaatccgg attgagtttt
     8101 tggataaatt caaacgaaac aggctgaccg tgaccggtat ttttaatgct agaatctgcc
     8161 attggaatcc ctaaattatc gtgaatccaa cttaaacgat aacctgaacc aaatgcagaa
     8221 attttgccac cattgactaa aataatcagt ccgttacctt taccttttac tgctgctttg
     8281 gtttggtcta ataaagtatt aatttctgct tttagttttt cggtttccgc ttctttatta
     8341 aataatttgc cgaaagaatc aagacgctgt aatccgcttt cgattaattt atcgccatta
     8401 tccgtcatgt cgatggtttt cgcaatttgg ctgacttcat caaatttttt tgctgtgcgt
     8461 gtgccaacaa tgatcaaatc cggatttaaa gaatttaacg cttctaaatc cggctcaaat
     8521 accgtaccga cattcaccgc tttgtctgca ctcggtttaa gataagaaag caccttaaat
     8581 tcaggcatac cgtcaatttt tacacctaag gcttgtaaag tatccaaact tccggtgtca
     8641 aacacggcaa ttttagacgg ggtttctttg aaagtaacct ctccacgtgc agttggaata
     8701 gtaacatctt tggcaaatgc agaagcagca aacacagcag ttgcggtcaa tgcaaacaag
     8761 gcttttttaa tcattggata actccattta tattgaaaat aattctcaaa gattttaacg
     8821 aaaattgatt ttaagtaaag cggaaaataa caagcgggcg aattttgcct aaaatttgca
     8881 aatttcaatt taattaaaat tccgatacac tatgtacatc aatacgtcat tatagcaaga
     8941 gaaaaatatg gcagaatata gcggaacaac ccaagagaag atgaaagata agcaggttga
     9001 gcaaattaat atctcaccac attcgattga ggcagagcaa gcggtgcttg gtggcattat
     9061 gctcagtaat gatcactggg ataatgttgc tgagcgtgta caaccgcacg atttttacaa
     9121 ctatgcgcat cgaattattt ttgaacaaat ggcggagttg gtgcgccaca ataaaccgat
     9181 tgatattatt acgctggatc aagccttaaa agacaaaggg gtgctgcaag atgtaggagg
     9241 ttttgcctat ttagccgagc tttctaaaaa taccccgagt gcagcaaata ttttagccta
     9301 tgcggatatt gtgcgagatc gttccgattt aagaagtgta atcagtgccg gcaatcaaat
     9361 tgcggaaatg ggctacaaaa cgaaagggcg taactccaaa gaagtgttag acgaggcaga
     9421 gcgaattgtg tttgaaattg cagaaaaacg caataacgcc aatgaaggtc cacaacgtat
     9481 tgacgatatt ttgcttaaaa cactcgctcg aatggatacg ctatctaaaa atcgccataa
     9541 cggcggagtt actggtgttt ctaccgggtt tgttgatctc aataaaaaaa ccgcagggtt
     9601 acagccttcg gatttaatca ttgtggcagc ccgtccttca atggggaaaa ctacttttgc
     9661 aatgaactta tgtgaaaatg cctcattagt ggatattgaa gataaagatg atttagacga
     9721 acagggtaat ccgaagaaaa aaccggcaaa gccagtgcta attttcagcc tggagatgcc
     9781 ggcagaccaa attatgatgc gtatgcttgc ctcactttct cgggtcgatc aaaccaagat
     9841 ccgaaccggt cagatttcag atgatgaaga cactgctaaa atctccagca caatggcaat
     9901 tttacaagaa cgtaataata tctatattga tgatagctcc ggtttaacac cgaccgaatt
     9961 acgttctcga gcgagacgga tttatcgtga aaatggcgga ttaagcttga ttatggtgga
    10021 ctatctccaa ttaatgcgag cgccgggatt tgcagataat cgtactttag aaatcgcaga
    10081 aatttcccgt tctttaaagg cactagcgaa agaattagaa gtgccagtag tcgctctttc
    10141 tcagctaaac cggagtttgg aacaacgtgc cgataaacgt ccggtaaact cggatttgcg
    10201 tgagtcaggt tctattgagc aagatgcaga cttaattatg tttatttatc gtgatgaggt
    10261 ctataacgac aattccgatc tgaaaggcat tgcggaaatt attatcggaa aacaacgtaa
    10321 cggcccgatt ggtcgggtgc gtttaacgtt ccaagggcag tattcccgtt ttgataatta
    10381 cacaggaggg cattatgatg atgaatacta aaccaaccca gccaatcaca ctttgtgcga
    10441 tttatcgcag taaggcaaaa gaaggaatgt atttatatgt gcctaagcgt gaccaatttg
    10501 agcaagtgcc ggaaacatta cgccagttat ttggtaagcc tgaatttgtg atgatgttta
    10561 atttaaccgg tgaaaagcca ttggtgcgtg ctaaaaacga agaagtgtta caattactta
    10621 tagagcaagg ctactatttg caaatgccac ctccgccgga gaatttgcat aaagccttta
    10681 tcgcacaaca gaaaggggaa aaataatgcg ctgcccattt tgttccactg aagaaactaa
    10741 agtgatcgat tcacggcttg cagcaagcgg ttatcaaatt cgccgccgcc gtgaatgtat
    10801 aagctgtaaa gaacgtttta ctacctttga atcggccgaa ttagttgtgc cttatgtgat
    10861 caaaaataat ggtagccgag agccgtttga tgccgaaaaa ttaagaacag ggttaagacg
    10921 tgcattaaat aaacgccctg tcagtgctga tgatgtcgaa aaagcaatta gccaaatcat
    10981 tattcagctg caagcgatgg gcgaacgaga agtgccgagt cgccatatcg gtacattggc
    11041 aatgaacgca cttaaacaat tagacaaagt agcatatatc cgctttgcct ctatttattt
    11101 aagctttagt gatattgaag agttcattaa agaaattgaa aaattaaaag agtagttgca
    11161 aaaaaacata aaaaaatgac cgcttgtgag tttaaaatcc acaagcggtc atttttcttt
    11221 aaaaatactc aattagcttt tacttgttcc aacacaaacc ctacaaacgc ccgcacaatg
    11281 ctagacggat aacgatgggc ggcataaatc atcgtcaaat caaatttggg gttttctgtc
    11341 tcacccaaga cttccaccaa gccattttct gccaccaatc tatccaccgt gctgtcggca
    11401 agcagagcca ctgctttttg ccatatattc aatggcatag ctgtcattgc tggaaaaaag
    11461 atagggaatt tctaccgctt gtctgccagt gcctagcgtg atgacgcttg cttcattttt
    11521 tgcccaagcg gcaatgggga aatttttcaa atctttgacg gtgctgggcg tgccaaagcg
    11581 tgccaaaaat ttagggtgtg ccacccattt actgccgatt tgccccactt ttttggcgat
    11641 aaactgttcg ccgtgtagtt gcccaatccg aaacgccaca tcaatgccat ctgccgacaa
    11701 atccaaaaga cggtcggtca gcgtgcaatg cacttgtact tgtggataag ctgcctgaaa
    11761 atcagctatc cacgccaaaa caggctcgca ggcagggctt gccgacaggc gtaatttacc
    11821 ctttaaattg ccttgctctt caaacaacac ttctttggca gtggcaagct gttcaatgga
    11881 caaatgcacc tgttcataca ggcgttgccc tgccattgtg ggtttaacgc ctgatttttg
    11941 gcggtcaaag agttgaatgt taagctcttt ttccaaatcg gcaatttgac ggctaatggt
    12001 tgcaatcggc acattgagtc gctcggaggc tttggagaga ctgccgtgct ggataacggc
    12061 gacaaagagg cggagggtgt tgaggttcat tgtttttctc cccaatgttc aatttcttga
    12121 ataaaatgtt taatactgcc ttggacttgt tgatttttac catatagata ataaacgcct
    12181 gaaagttctt gtaaaaattt actttcttta taagcgtgtt tttcacccaa cacagtc
//
LOCUS       DANLZC010000076        11920 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.076, whole genome shotgun sequence.
ACCESSION   DANLZC010000076 DANLZC010000000
VERSION     DANLZC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11920
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.076"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..300)
                     /locus_tag="PXE44_001582"
     CDS             complement(<1..300)
                     /locus_tag="PXE44_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5589082.1"
                     /translation="MPFYAGQVKCIFIDPPYNTKSAFTHYDDNLEHSIWLSMMYPRLE
                     ILRELLAEDGSIWITLDDNESHYLKIMLDEIFGRKNFISNLIWEKKRKPSFLGKVG"
     gene            complement(368..535)
                     /locus_tag="PXE44_001583"
     CDS             complement(368..535)
                     /locus_tag="PXE44_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase N-4/N-6"
                     /protein_id="HDL5589083.1"
                     /translation="MTTLTWYGKEEAVKKAKQTPYCLLRELPDFSHNIARQPTADSRQ
                     PTADSRQLSFC"
     gene            complement(676..3162)
                     /gene="torA"
                     /locus_tag="PXE44_001584"
     CDS             complement(676..3162)
                     /gene="torA"
                     /locus_tag="PXE44_001584"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL5589084.1"
                     /translation="MKKQDNIDVSRRGFLKNSSLSLAAGAGLASGTTGVVGALASTSA
                     KAAEMKTVVTAAHWGPLGVVVENGKVVKSDSVFEISVPNELQHVVADQVHGETRVKYP
                     MVRKGYLEGNKDTTLRGRDEWVRVSWDKAFELVANEMKRVREAHGANAIFGGSYGWYS
                     SGALHASRTLLQRYLNVTGGFVGVKGDYSTGAAQVIMPHVLGTIEVYEQQTSWEVILE
                     SSDIVVLWSANPLNTLRIAWTSTDQQGIEYFKKLKESGKRIICIDPVKSESCEFLGAE
                     WVPVNTGTDVALMLGMAHTLVAEGKHDQAFLKKYTTGFEKFEEYLLGKTDNQPKDAKW
                     ASQICGVPEETIKKLATDFQSKRTMLMAGWGIQRQRHGEQGHWMLVTLAAMLGQIGLP
                     GGGFGLSYHYSNGGVPSATGGILGSITANPAVEAGAKTWLDETSKSSFPVARISDALL
                     NPGKTIDYNGTQITYPDIKIVYWAGGNPFTHHQDTNTLVKAWQKPDTIIVNECHWTPT
                     ARMSDIVLPATTSYERNDLTMSGDYSMMNIFPMKQVVEPQFEAKNDYDIFAELAKRAG
                     KEAEFTEGKSEMDWLQSFYQTAFEAARKNRVIMPKFEKFWQENKPLSFKASDKAKKWV
                     RYEEFRNDPLLNPLGTPSGKIEIFSEVIAKMNYDDCKGHPTWMEHEEYSGKTTAEEPL
                     ALVTPHSKWRLHSQLAYSSLRKQYAVNDREPVLIHTEDAAARGITSGDIVRIFNKRGQ
                     VLAGAVVTDGIIKGTLALHEGAWYDPLDLGTSEQPLCKNGNPNVLTRDEGTSKLAQGN
                     SPNTATVQVEKFTGQAPEVTVFKEPKYTQG"
     regulatory      complement(3181..3377)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(3381..4496)
                     /locus_tag="PXE44_001585"
     CDS             complement(3381..4496)
                     /locus_tag="PXE44_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL5589085.1"
                     /translation="MAKMKKIAVFVGIMALGAAVLGGTQYAMKATSSTEFCVSCHSMT
                     HPQAEWEGSVHFSNRKGIRAECSDCHLPQDGLHYVKAKIIALKDVWHTVVTNKLPDQE
                     AYEAHRLEMAQRVWAEMKENDSMTCKSCHSFDAMVLSEQTEAAQKMHKLAQETNQTCI
                     DCHKGIAHFMPDVPVDNAAAMGELAKHGAEFSTNDKTLYTLAMTPVQLAQGGEIRLLP
                     YAELHQWTAEGEQVKATVNGWQQEGAESVVYMDLGKRIMVALLPDDAQSKVNVTKTVF
                     DNVTNSNWKEINFEITAPKNAVTANITALNHFGDNLNQTHCSGCHAPIGAEHYTANQW
                     IGVVNSMKDRTSMSADDVRAVTIYLQRNAKDVAGSSH"
     gene            complement(4638..6389)
                     /locus_tag="PXE44_001586"
     CDS             complement(4638..6389)
                     /locus_tag="PXE44_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5589086.1"
                     /translation="MDKRSRWSCLVNEQGEISEWEDKAGADYFAELPQICHYAQALLY
                     SDKNSLQFYYSSNHQWLKISLERHQSFVLTQAEHTDLPYGLSKREIELLTLISSGLSN
                     EDIANELCISKRTVDKHIENIFAKTHTKSRTLLAVFAITEHLICLPTPGKLTQSILAS
                     FNIEQLALQIIAKKQPLVTASTRHEPPIFTINHHHSRPIIIGVPYVEQGIGQIDTREL
                     LNGTQLAVETINQQGGIHGREVQVATAGFYVKDKASILQAYHHLFDQEVDAISTAYAC
                     YLPEVHELIATKGIPYLHIATHSGSNKLAQNLPNQRIENMFQVCASDVTYGLGISHFL
                     QYYRYHYAPWLQNKTVMVVTVSWQKVDIGIESLTLSLREQGWNVDYLELTQEQDNYAT
                     LMAQVHNLAPSIIVFASYFAEDIVQFYQTFIRNPTNAIIYSIYAPSAFLPHEKLCEGV
                     LWSTNSGLSENYLGRQFSQHYERFFGKSPTYSQASIAYDQANILANAWKQSVSPRHFK
                     AVSNAIRLQPHYGVNGTYYFNTDSQIGLTYAETHDLSISLPQLVYQIQQGQSRVIAPE
                     LFANAQFILPPWFSEKA"
     regulatory      6553..6709
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            6698..7723
                     /gene="moaA"
                     /locus_tag="PXE44_001587"
     CDS             6698..7723
                     /gene="moaA"
                     /locus_tag="PXE44_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDL5589087.1"
                     /translation="MQSIIPIKNVGDDVVSGLVDRFQRQYTYLRLSITDVCNFRCNYC
                     LPDGYKPPSHKQTFLSVDEIQRVAKAFANLGTEKIRITGGEPTLRKDFLEIAHTISQT
                     PGIRKVALTTNGYRLERDVELWQQAGITDINVSVDSLDPRQFQLITGENKLQSILKGI
                     DKAFEIGYPKIKVNAVLMKQYTAPELDKFLTWIKDKPIQMRFIELMETGEMDSFFQAQ
                     HLSGQSVMTRLLQEGWQLQPKAMSDGPAKVLSHPDFKGEIGLIMPYEKNFCASCNRLR
                     VSALGKLHLCLFGEEGIDIRDLLASDEQQLQLETRLKSSLQGKREHHYLHIGDSGVRN
                     NLASIGG"
     gene            8224..8700
                     /gene="moaC"
                     /locus_tag="PXE44_001588"
     CDS             8224..8700
                     /gene="moaC"
                     /locus_tag="PXE44_001588"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDL5589088.1"
                     /translation="MTTFTHINQNGEANMVDVSMKQETVRIARAEAFVRMNAETLQMI
                     VSGNHHKGDVFATARIAGIQAAKRTWELIPLCHPLLLSKVEVQLEALPESNQVRIESL
                     CKLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMIIENVRLLSKSGGKSGDFKVD"
     gene            8693..8938
                     /gene="moaD"
                     /locus_tag="PXE44_001589"
     CDS             8693..8938
                     /gene="moaD"
                     /locus_tag="PXE44_001589"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDL5589089.1"
                     /translation="MIKILFFAQVRELVATDRLDVEPTFTTAEALREHLSQQGKKWQL
                     ALEKGKLLVAINQTICPLETEIKDGDEVAFFPPVTGG"
     gene            8940..9395
                     /gene="moaE"
                     /locus_tag="PXE44_001590"
     CDS             8940..9395
                     /gene="moaE"
                     /locus_tag="PXE44_001590"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDL5589090.1"
                     /translation="MFETLIEVQEAEFDQNRIYQWLSEQHSVGAATLFIGKVREMNLG
                     DNVSGLFLEHYPAMTKKALQEIVAQARTRWDLQRVAVIHRIGQLHTGDEIVLVGVSSA
                     HRGDAYHANEFIMDYLKTKAPFWKREQTQAGERWIEGRESDQQAAKKWQ"
     gene            9428..9910
                     /gene="ybeY"
                     /locus_tag="PXE44_001591"
     CDS             9428..9910
                     /gene="ybeY"
                     /locus_tag="PXE44_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL5589091.1"
                     /translation="MENLYIDLQIACENTENLPSEQQFYTWVQKALAVEAKTDDFPES
                     EITIRIVDEAESHELNLTYRGKDKPTNVLSFPFEAPEGIEMPLLGDLVICRQVMEKEA
                     EEQGISAESHWAHLAIHGTLHLLGYDHIEEDEAVEMESLETEIMQSLGYEDPYLSEKI
                     "
     gene            9988..>11920
                     /locus_tag="PXE44_001592"
     CDS             9988..>11920
                     /locus_tag="PXE44_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HDL5589092.1"
                     /translation="MRTIAIITSPNNADFIQQGYFYIPENHQLKAIPFSNAKTILGQE
                     YPFAIYTMQAENGVNFNLDAFAILAGTIQENGTLFLLCPQWDNLENELDFDALRWNEN
                     QAIACPNFYQHFKQLVAKFGFEVRTNLPKLPATSGQIPSKIYQLTQEQQNILQNLPLD
                     PADIHLITAPRGRGKSTLAGRLAKALPAQNKVLITARSYSALPSFWRSAEQKIPFFAP
                     DNLIEQVKSKNISPNQWLFIDEAASLPLPILHTLCAYFEKVVLTTTTQNYEGTGRGFE
                     LKFPVMLDKPFKHWTLSQPLRWSENDPLEAFVTELLLLSEEKSPLFSKEGKGEIYSLR
                     SHYVPLLKQRSNAKRLWQKQLSKNISYSNKHLQEKIAFYHLLANAHYKTTPTDLRRLF
                     DGENQLLFSQQENEQLIGGIWAMQEGGLDEKLTQAIWLGERRPQGSLVAQYLCFQGNL
                     PQACKLHSIRISRIAVLPEKQNQGVGKRLISQFILQNSVEKRPLDFISVSFGLTTPLH
                     AFWQSCGFQLVQITPNKEASSGYQSAMMIYPISEQGKLFAQQATQAFARDFALQPFFT
                     EICEELQNFEQFRPLATHNLDERDWKNLTAFAHGKRTFSAAYVSLKRLYLSDKARFSE
                     LDIFERKGQKISKMEVENYR"
BASE COUNT         3432 a         2531 c         2489 g         3468 t
ORIGIN      
        1 accaactttg cctaaaaaag atggtttcct tttcttttcc caaattaagt tagaaatgaa
       61 attcttccgc ccaaaaattt catcaagcat aattttcaaa tagtgacttt cgttatcatc
      121 aagagtgatc caaattgaac catcttcggc aagtaactct cgcagaattt caaggcgtgg
      181 atacatcatt gaaagccaaa tggaatgctc taaattatca tcgtagtggg taaaggccga
      241 ttttgtgtta tagggaggat caataaaaat acatttgact tgccccgcat aaaagggcaa
      301 aagagatttt aaggctgata aattatcacc ctgaataatt aaattttgct tgactttaga
      361 taaggtttca gcagaagctg agctgtcggc tgtcggctgt cggctgtcgg ctgtcggctg
      421 tcggctgtcg ggcaatatta tgcgagaagt ccggcaactc acgcaatagg caatatgggg
      481 tttgttttgc ttttttgacg gcttcttctt tgccgtacca agtaagggtt gtcataaaat
      541 gcacctgaaa agtaaaagtg gattattgta acaagcggtc aaaaaaatag aaagttttac
      601 aaaaaattaa gcaaatttaa ccgcttgcaa catagaaaag gcacagtttt atactgtgcc
      661 tttggttata tgaaattagc cttgtgtgta tttaggctct ttaaatactg ttacttctgg
      721 tgcttgtccg gtgaattttt caacctgtac cgtagcggta tttggtgagt taccctgtgc
      781 taattttgat gtgccttcat cacgggttag cacgttaggg ttgccgtttt tacatagcgg
      841 ttgctcgctg gtgccgagat cgagcggatc gtaccaagct ccttcgtgca aggctaatgt
      901 acctttgata atgccatctg tcaccaccgc accggctaat acttgaccac gtttgttaaa
      961 aatacgtaca atatcaccgc ttgtaatgcc acgagcggcg gcatcttcag tatggatcaa
     1021 caccggctca cggtcattaa ccgcatattg tttgcgtaat gatgagtaag ctaactggct
     1081 gtgtaaacgc catttagagt gtggagtcac caatgccaac ggttcttctg cggtggtttt
     1141 acccgagtat tcttcgtgtt ccatccaagt tgggtgacct ttacagtcat cataattcat
     1201 tttggcaatc acctcagaga aaatctcaat tttgccggac ggtgtgccta atggatttag
     1261 gagtgggtca ttacgaaatt cttcataacg aacccatttt ttcgctttgt ctgatgcttt
     1321 aaaagaaagt ggtttattct cttgccagaa tttctcgaat ttcggcataa tcacacgatt
     1381 tttgcgtgct gcctcaaatg cagtttgata gaaactttgt agccaatcca tttcagattt
     1441 gccttcggta aattccgctt ctttgcctgc acgtttggct agttcggcaa aaatatcgta
     1501 gtcgtttttt gcctcaaatt gcggttcgac cacctgtttc atcgggaaaa tattcatcat
     1561 tgaataatcg cccgacatag ttaagtcatt acgctcatag ctggtggttg ccggcagcac
     1621 gatgtcagac atacgagccg ttggtgtcca atggcattca ttgacgataa tagtatccgg
     1681 tttttgccac gctttcacca aagtgttggt gtcttggtgg tgggtaaacg gattaccccc
     1741 tgcccaatag acaattttga tatcaggata agtaatttgt gtgccattgt aatcaatggt
     1801 tttgcccggg ttgagcagtg catcggaaat acgagccacc gggaatgaag attttgatgt
     1861 ttcatctaac caggttttcg ctccggcttc tactgccgga tttgctgtga tagagcctaa
     1921 aatgccccct gtggcactcg gcacgccgcc gtttgagtaa tggtagctta aaccgaaacc
     1981 accacccggt aagccgattt gacctaacat tgcagctagg gtgactaaca tccaatgccc
     2041 ttgttcaccg tgtcgttggc gttgaatccc ccagcctgcc attaacattg tgcgtttaga
     2101 ttggaaatca gtcgctaatt ttttaatggt ttcttccggc acaccacaaa tttgacttgc
     2161 ccattttgca tctttcggtt gattgtctgt tttaccgagc agatattctt caaacttctc
     2221 aaaaccggtg gtgtattttt taaggaatgc ttgatcgtgt ttgccttcag cgactaaggt
     2281 atgagccata ccgagcatta acgcaacatc agtgccggtg tttaccggta cccattctgc
     2341 acctaaaaat tcgcaagatt cactttttac cgggtcaata cagataatgc gtttcccgct
     2401 ctctttgagt tttttgaagt attcaatacc ttgttgatcg gtagaagtcc acgcaatgcg
     2461 tagcgtattt aacgggttag ctgaccaaag caccacaata tctgaacttt ctaaaatcac
     2521 ttcccaacta gtttgctgtt cgtacacctc gattgtaccc aagacgtgtg gcataatcac
     2581 ttgagcagca ccggtagaat agtcgccttt tacaccaaca aatccgccgg taacatttaa
     2641 atagcgttgt aacagtgtac gggaagcgtg taatgcccca gaactatacc aaccgtaaga
     2701 accaccaaaa atagcattcg ccccgtgagc ctcacgaaca cgtttcattt cattcgcaac
     2761 taattcaaat gccttatccc acgaaactcg cacccattca tcacgcccac gtagagtagt
     2821 atctttatta ccttctaagt agcctttacg caccattggg tattttactc gtgtttcacc
     2881 gtgtacttgg tctgctacca cgtgttgcaa ttcatttggc acagaaatct caaaaacaga
     2941 atcagatttc accactttgc cattttctac cactacacct aaaggccccc agtgggcggc
     3001 agttactact gttttcatct cggctgcttt ggcactggtg gaagctaatg cccccactac
     3061 accggtagta ccactggcta aacctgcacc ggcagctaag cttaatgatg aattttttag
     3121 aaaaccgcga cgacttacgt caatattgtc ttgttttttc ataataaacc tcgtttgaag
     3181 ttaagttaaa aatagacacc tttctaaagt cgagtggata aaccatttct cgtgtatcca
     3241 ttggtaaaag caccgacggt aatttttgca aaatgttagg aaaaaatgac cgcttgtagt
     3301 aggtgcttta cggcttgcta ccatatctct gctctcagca gacacttagg ctcacaagct
     3361 cggtatcctc taagttgcaa ttaatgggaa ctgcctgcta catcttttgc attacgttgg
     3421 agatagatag tcaccgcacg cacatcatca gccgacatag atgttctgtc tttcattgaa
     3481 tttaccaccc caatccattg atttgcggtg taatgttctg ccccaatcgg tgcatggcaa
     3541 ccgctacagt gtgtttggtt taagttatcg ccaaaatgat ttagtgcagt aatattggca
     3601 gtgactgcat ttttaggagc agtaatttca aaattgattt ctttccaatt tgagttggtg
     3661 acattatcaa acactgtttt tgtgacattc actttgcttt gagcgtcatc aggtagaaga
     3721 gcaaccataa tacgcttgcc taaatccata tacaccacag attccgcacc ttcttgttgc
     3781 cagccattaa ccgttgcttt aacctgctcg ccttctgctg tccattgatg taattctgca
     3841 taaggtaaca agcgaatttc tccaccttga gcgagctgca ctggagtcat tgcgagcgta
     3901 taaagggttt tatcatttgt gctaaattcc gcaccgtgtt tagctaattc gcccattgct
     3961 gcggcattat ctaccggcac atcaggcata aaatgggcaa ttcctttatg gcaatcaata
     4021 caggtttgat tagtttcttg ggctagtttg tgcattttct gtgccgcttc tgtttgttca
     4081 gaaagtacca tcgcatcaaa actatgacag cttttacagg tcatagaatc gttttctttc
     4141 atttctgccc aaacacgttg tgccatttct agacgatggg cttcataggc ttcttgatca
     4201 ggtaatttat tggttacaac ggtgtgccaa acatctttta atgcgatgat tttagctttg
     4261 acataatgca agccgtcttg tggtagatgg caatccgaac actcggctcg aattccctta
     4321 cggttggaaa aatggacact gccttcccac tctgcctgtg ggtgagtcat agaatggcag
     4381 ctcacacaaa attctgttga gctggtggct ttcattgcat attgtgtgcc acctaatacc
     4441 gcagcaccta atgccataat accgacaaat acagcaattt ttttcatctt agccataaat
     4501 taccccactt aatacactaa aaattaaaga cagaggcatt gtattaactc ccaagaggta
     4561 taaaaataag taaaaatacc tattacctcg aaagagtgag aataatttga tctaaggcaa
     4621 aaatatgtag tgaaatttta ggctttttca gagaaccacg gcggtaagat aaattgtgca
     4681 ttggcaaaca actccggtgc aatcacacgg ctctgccctt gttggatttg gtaaaccaac
     4741 tgtggcaagc tgatagataa gtcgtgggtt tctgcgtaag ttaatccaat ttggctatcg
     4801 gtattaaaat agtaagtgcc gtttacccca taatgtggct gtaagcggat cgcattggag
     4861 acggctttaa aatgccgtgg tgaaacactc tgtttccacg catttgccag aatattcgct
     4921 tggtcataag caatactggc ttgggaataa gtgggcgatt tgccgaaaaa ccgttcgtaa
     4981 tgttggctaa actgtctgcc taaataattt tcgcttaacc cactattggt actccaaaga
     5041 acgccttcgc aaagtttctc gtgtggcaaa aacgccgaag gtgcataaat actgtaaata
     5101 atggcatttg ttgggttgcg aatgaacgtt tgataaaact gtacaatatc ttctgcaaaa
     5161 taagaggcaa acacaataat ggaaggtgcg agattatgca cctgtgccat taatgtggca
     5221 taattatctt gctcttgggt gagttccaga taatccacat tccagccttg ctcccgcaaa
     5281 cttagggtta aactttcaat accgatatca actttttgcc aactcaccgt taccaccatc
     5341 accgttttat tctgaagcca aggggcataa tggtaacggt aatattgcag aaagtgagat
     5401 atacctaagc cataggttac atcactggcg caaacctgaa acatattttc aattcgttga
     5461 ttcggtaaat tttgggcaag tttatttgag ccactatggg ttgcaatatg caaataagga
     5521 atgcctttag ttgcaattaa ttcgtgtact tccggcaagt agcaggcata agcggtggaa
     5581 atcgcatcaa cttcttgatc aaataaatga tggtatgcct gaagaatact tgctttatct
     5641 ttaacataaa acccagccgt tgcaacctgt acttctctgc cgtgaatacc accttgttga
     5701 ttgatggtct caaccgcaag ttgagtgccg ttaagtagct ctcgggtatc gatttgccca
     5761 ataccctgtt caacataagg cacgccaata ataatcggac gactatgatg gtggtttatc
     5821 gtaaaaatcg gtggttcgtg tcgagttgaa gccgttacaa gcggttgttt tttcgcgata
     5881 atttgcaatg ccagttgttc aatgttaaag ctggcaagaa tggattgggt tagttttccc
     5941 ggtgtgggta aacagattaa atgttcggta atggcaaaaa cagctaataa ggttcggctt
     6001 ttggtatgtg ttttcgcaaa aatattttcg atatgtttat caacagtccg tttgctgata
     6061 caaagctcat tggcgatatc ctcattgctc aaaccactgc taattagagt gagaagctca
     6121 atctcccttt tactcagtcc ataaggcaaa tcggtatgct ctgcttgagt caatacaaac
     6181 gattggtggc gttctaggct gatcttaagc cattgatggt ttgaactata gtaaaactgc
     6241 aagctatttt tatctgaata gagcaatgcc tgtgcatagt ggcaaatttg cggtaattca
     6301 gcaaaatagt ctgcccctgc tttgtcttcc cattctgata tttcgccttg ttcatttact
     6361 aaacaagacc atcggctacg tttatccatt ctcacctcga actaaaaacg ttagctaaaa
     6421 tagcaaaatt atctcggcat taatatgata taaatcaaac taagcaacgg ttgccagatt
     6481 tttataggta gagcatacaa tttaaattat acctcttgcc taattaggtt tctcaccata
     6541 aaatgaaaac caatttattg aagactccga gcattatttc ctaactctaa aaattaaaga
     6601 caaatttttg atatggaatt tatgccagta acagcataaa gctgttcaaa ggataactta
     6661 gaaatgggtt gtcctctcgt gtttagaaag gtgtcaaatg cagtctatta tcccaattaa
     6721 aaacgtgggt gatgatgtcg tttcaggttt agttgatcgc tttcagcgtc aatatactta
     6781 tttgcgacta tccattactg atgtttgtaa cttccgttgt aattattgct tgcctgatgg
     6841 ctataagccg ccttcacata agcaaacttt tcttagcgtt gatgaaatcc agcgggtggc
     6901 taaagccttt gccaatttag ggacggaaaa aatccgtatc accggtggtg agccaacttt
     6961 acgcaaagat ttcctcgaaa tcgctcacac tatttcacaa acacccggta tccgtaaagt
     7021 tgccctaacc accaacggct atcgcctaga gcgtgatgtt gaactgtggc aacaagccgg
     7081 tattaccgat attaatgtga gtgttgatag ccttgaccca cgccaatttc aactgattac
     7141 cggcgagaac aagttgcaat ctattttaaa gggtattgat aaagcctttg aaattggcta
     7201 cccaaaaatt aaagtcaatg cggtgctaat gaagcaatat accgcccctg aattggataa
     7261 attcctcact tggatcaaag acaagccgat tcaaatgcgg tttattgagc tgatggaaac
     7321 cggtgaaatg gatagtttct tccaagctca acatctttct ggtcaatcgg tgatgacccg
     7381 cttattacaa gaaggctggc aattacaacc caaagcgatg tctgacggcc cggcaaaagt
     7441 gctttctcac cctgatttta aaggcgaaat cggcttaatt atgccctacg agaaaaattt
     7501 ctgtgcctct tgcaaccgct tgcgtgtttc cgcacttggt aaactgcatc tttgcttatt
     7561 tggtgaagaa gggattgata ttcgtgattt actggcaagc gatgagcaac agttacagct
     7621 tgaaacccgc ctaaaatctt ccttacaagg caagcgagaa caccactatt tgcatattgg
     7681 ggatagtggg gttaggaata atttggcgag tatcggtggt taaacaacca ccgagcgtgt
     7741 tttttctgtt acttttcttt gcttcgccaa agaaaagtaa ccaaaagaaa ggcgacccca
     7801 gattttcctt aatccttcgc ttgttgctaa cttttgggac gaacttttca aactcgccaa
     7861 ctgcgttggc tcaaacacga aaagttgtcc tcaaaagttt acgcaatcgc tacggcggaa
     7921 aataggggca agcggtacat tatttaagat ttttgcaagt ttttatttaa aaataaccgc
     7981 ttgtaatgtt ccagcttctc ccttctgaac cgcctgagcg actttgaatt tataggaaaa
     8041 atttagtgtt tgagccaacg aagttggcga gttttaaatt tttccgttaa acaaattcaa
     8101 ataaaatgaa gatagcggtg aagtagggtg tgctttcttt ggttactttc tttgcacaag
     8161 caaagaaagt aacaagaaga cgctcaatat taattttaca atcaaattta aagagacaaa
     8221 caaatgacaa ccttcaccca catcaaccaa aacggcgaag ccaatatggt tgatgtttca
     8281 atgaaacaag aaaccgtacg cattgcccga gcggaggcat ttgtgcgaat gaatgccgaa
     8341 acattacaaa tgatcgtcag tggtaatcac cacaaaggcg atgtattcgc aaccgctcga
     8401 attgccggta ttcaggcggc gaagagaact tgggagctga ttccactctg ccacccgtta
     8461 ttgctttcta aagtggaagt tcaactggag gcgttgccgg aaagcaacca agtgcgaatt
     8521 gaatcactct gtaaactaac cggcaaaacc ggcgtggaaa tggaggcatt aaccgcagct
     8581 agtgttgctg ctctgactat ttacgatatg tgtaaagccg tgcaaaaaga tatgattatt
     8641 gaaaatgtaa gattacttag taaaagtggc ggaaaatcag gagatttcaa ggttgattaa
     8701 gatacttttt ttcgcccaag tgcgtgaact tgtggcaacg gatagactag atgttgagcc
     8761 tacttttacc actgccgaag cgttgcgtga acacttaagc caacagggta aaaaatggca
     8821 acttgccctt gaaaaaggca aactgctggt tgcaattaat caaacgatct gcccgcttga
     8881 aacggagatc aaagacggcg atgaagtggc attcttccca ccggtaaccg ggggctaaaa
     8941 tgtttgaaac cctgattgaa gtacaagaag ctgaatttga ccaaaaccgc atttaccaat
     9001 ggctcagtga acaacatagc gtgggggcag ccactctatt catcggtaaa gtacgtgaaa
     9061 tgaatttggg cgataatgtt tccggtttgt ttcttgaaca ctatccggca atgactaaaa
     9121 aagcattgca agaaattgtg gctcaagctc gcactcgttg ggatttacaa cgcgttgcgg
     9181 tgattcaccg cattgggcaa ctgcataccg gtgatgaaat cgtattagtc ggtgtgagtt
     9241 ctgctcatcg aggtgatgcc taccacgcca acgagtttat tatggattat ctcaaaacca
     9301 aagccccatt ttggaaacga gaacaaaccc aagccggtga acgttggatt gaagggcgtg
     9361 agagtgacca acaagccgcc aaaaaatggc aatagaaaaa atggcaataa tttgcaaaag
     9421 gtaacgaatg gaaaatttat atattgattt gcagatcgcc tgcgaaaata ccgaaaactt
     9481 accaagtgaa caacaatttt atacttgggt acaaaaagcc ctcgcagttg aggcaaaaac
     9541 cgatgatttc cctgagagtg agatcaccat tcggattgtc gatgaagcag aaagccacga
     9601 gctaaatctg acttatcgtg gtaaagacaa gccaaccaat gtgctatctt tcccgtttga
     9661 ggcccccgaa ggaatcgaaa tgccactatt gggtgattta gtcatctgtc gtcaagtaat
     9721 ggaaaaagag gcggaagaac aaggtatttc agccgaatcc cattgggctc atttggcgat
     9781 tcacggcaca cttcatctgc tcggctacga ccatattgag gaagatgaag cagtggaaat
     9841 ggaaagcctc gaaaccgaaa ttatgcaaag tttagggtat gaagatccgt atctttcaga
     9901 aaaaatatag tcttgtaggg gagggtcctg tgccctccag tactccatta taatcggaca
     9961 ggcacaggag ctgttcctac aagttatatg agaacgatcg caatcataac atctcctaac
    10021 aatgccgact ttattcaaca aggctatttt tacattcccg aaaatcatca gcttaaagct
    10081 atcccctttt ccaatgccaa aacgatttta ggacaagaat acccttttgc catttatact
    10141 atgcaagcag aaaatggggt gaattttaac ttagatgctt ttgcaatact tgccggcact
    10201 attcaagaaa acggtacttt atttttgctt tgtcctcagt gggataattt ggaaaatgaa
    10261 ttagatttcg atgcgttacg ttggaacgaa aatcaggcta ttgcctgccc gaatttctat
    10321 caacatttta aacaattagt cgctaaattt ggttttgaag tcagaactaa tttgccaaaa
    10381 ttaccggcta caagcggtca aattccttca aaaatttacc aattaacgca agaacagcaa
    10441 aatattttgc aaaatctacc gcttgatcct gccgatattc atttgattac cgcccctcgt
    10501 gggcggggca aatctaccct tgcagggcga cttgcaaaag cgttacccgc acaaaataag
    10561 gtattaatta ccgcacggtc ttattctgca ttgcccagtt tttggcgttc ggcagagcaa
    10621 aaaatccctt ttttcgcacc tgacaattta atcgagcaag ttaaaagtaa aaacatttca
    10681 cctaaccaat ggctctttat tgatgaggca gctagcctac ccttgccgat actccacaca
    10741 ctttgtgcct attttgaaaa agtggtttta accacaacaa cccaaaatta cgaaggcacg
    10801 gggcgaggat ttgagctgaa attcccggta atgttggata aaccgtttaa acattggacg
    10861 ttatctcaac cattgcggtg gagcgaaaat gatcctctcg aagcatttgt gactgagttg
    10921 ctattacttt ccgaagaaaa atctcccctc tttagcaaag aggggaaggg ggagatttat
    10981 tcgcttcgct cacactatgt gccgttgcta aagcaacgtt caaacgcaaa gcgtttgtgg
    11041 cagaagcaat tatcgaagaa tataagttat tcgaataagc atttacagga aaaaatcgct
    11101 ttctaccact tactcgccaa cgcccattac aaaaccactc caactgactt acgccgttta
    11161 tttgatggcg aaaatcagct tttgttttca caacaggaaa acgagcaact tatcggcgga
    11221 atttgggcaa tgcaagaggg tggtttagat gaaaaattaa cccaagccat ttggctaggg
    11281 gaacgccgtc cgcaaggcag tctagtggct caatatctct gttttcaagg gaatttaccg
    11341 caggcttgca aattgcattc cattcgcatt tctcggattg cggtattgcc tgaaaagcaa
    11401 aatcaaggcg ttggcaagcg gttgatttct cagtttattt tgcaaaattc ggtagaaaaa
    11461 cgaccgcttg attttatctc tgtgagcttt gggctaacta ctccactcca cgcattttgg
    11521 caatcttgtg gtttccaact tgtgcaaatc acaccgaata aagaggcaag cagtggttat
    11581 caaagtgcaa tgatgattta cccgatttcg gagcaaggaa agttatttgc acagcaggca
    11641 acccaagcct ttgccagaga tttcgccctt cagccatttt tcacggaaat ttgtgaggaa
    11701 ttgcaaaatt tcgaacaatt tcgaccgctt gccacacaca atttagatga acgtgattgg
    11761 aaaaatttaa ccgcgtttgc tcatggcaag cggacgtttt cagcggcgta tgtgagttta
    11821 aaacggcttt atttatcgga taaagcacgc ttttcagagc tggatatttt tgaacgaaaa
    11881 gggcaaaaaa tctccaaaat ggaggtggaa aattatcgaa
//
LOCUS       DANLZC010000077        11743 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.077, whole genome shotgun sequence.
ACCESSION   DANLZC010000077 DANLZC010000000
VERSION     DANLZC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11743
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.077"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            113..2005
                     /gene="mnmG"
                     /locus_tag="PXE44_001593"
     CDS             113..2005
                     /gene="mnmG"
                     /locus_tag="PXE44_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL5589093.1"
                     /translation="MIYNQIYDVIVVGGGHAGTEAALAPARMGLKTLILTHNVDTLGQ
                     MSCNPAIGGIGKGHLVKEIDAMGGLMAKATDVAGIQFRTLNNSKGPAVRATRAQADRV
                     LYRNAVRTALENQPNLDIFQQEVVDILVENNRAVGAVTKMGLTFKARAVVLTAGTFLA
                     GKIHIGLDNYAGGRAGDPAATMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQ
                     HGDEVLPVFSFMGDVSQHPRQIPCYITHTNDQTHDLIRNSLDRSPMYTGIIEGVGPRY
                     CPSIEDKVMRFADRNSHQIYLEPEGLNTIEVYPNGISTSLPFDVQMGIVNSMKGLENT
                     RIIKPGYAIEYDYFDPRDLKPTLETKAIDGLFFAGQINGTTGYEEAGAQGLLAGINAA
                     LQVQGKEAWYPTRDQAYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTP
                     KAHELGLIDEARWVRFNQKMEAIEQERARLKSTWAHLQMPNLDELNALLANPLAREAS
                     GEDLIRRPEMSYEKLTQIALFAPAIEDKQVAEQVEIAIKYQGYIEHQYNEIERHKRHE
                     NTLIPVEFDYDKVESLSNEVRAKLMQHRPVSIGQASRISGITPAAISILLVNLKKQGM
                     LKRGEL"
     gene            2100..2324
                     /locus_tag="PXE44_001594"
     CDS             2100..2324
                     /locus_tag="PXE44_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589094.1"
                     /translation="MIYLRKLGFSPFDHQMFAQPSWYFCNALVHTNYENLRQGVFVTK
                     AYLVMFLENLLFEKSHSLSNRALRINENND"
     gene            2337..2951
                     /gene="rsmG"
                     /locus_tag="PXE44_001595"
     CDS             2337..2951
                     /gene="rsmG"
                     /locus_tag="PXE44_001595"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL5589095.1"
                     /translation="MLAKLNRLLAQAEINLTDQQKEQLVGFVRLLDKWNKAYNLTSVR
                     NPDEMLVKHILDSLVVSRHLQGETFIDVGTGPGLPGIPLAIANPDKQFVLLDSLGKRI
                     TFIKNALRELGIKNVTPVQSRVEEYTESQFDGVLSRAFASLNDMIDWCYHLPTENGKF
                     YALKGVYDEQEIIEIKQPIQLVEVIKLEVPELVGERHLVVLKKP"
     gene            3219..4667
                     /locus_tag="PXE44_001596"
     CDS             3219..4667
                     /locus_tag="PXE44_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5589096.1"
                     /translation="MNRVTNKADEFAKLSTFERIAYGSGDLAQNLVFGTVGGFLLTYL
                     TTVNGISAVAGATIFLIVKWGNVFWDPFVGAFIDKSKPSAQGKYRPYLLKFGIPLVVL
                     ASLLFLPLESVRGSVIYAFLSYFATAMVYSFVNIPYGSLSASLTRDTDEIAKLTTTRM
                     TMANIANLFVYTLFPLFVQLLAPHAELKNTGFFGIELYLGDYMSAAAGPAWFKVYAVY
                     MVIGFVALMLSYFGTKERIIPTAEETDKVKYSDLVNELKRNRPLQILGLFFLIGFTFM
                     FFGNTVWPFYLKYNIGHSEWIASVGLLGSIPGIFLVFLWPKLRRTIGKKNFFYLFLGI
                     FVLGQLCLWVWQFDSFKYSPTLGYIGRFLQQWGLTSATGFMWALVPEVVTFGEFKSKK
                     RVAGIINALMGLFFKIGLALGGIIPGYINAVYGFNGSLLAQTEEALFGINLSMIWAPI
                     ILAIIAGFVMSRYPLSDTDIDHINEKITAYSK"
     gene            4690..5736
                     /locus_tag="PXE44_001597"
     CDS             4690..5736
                     /locus_tag="PXE44_001597"
                     /inference="COORDINATES: protein motif:HMM:NF016497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL5589097.1"
                     /translation="MQIQNPVLPGFNPDPSIVRVGDTYYIATSTFEWFPGVRIHESKD
                     LVHWNLVKNVLDTTTLLDMRGNPSSGGIWAPDLSYAEGKFWLVYTDVKITDGAFKDMT
                     NYLTTAKEIQGPWSAPIKLNGIGFDASLFHDDDGRKYLVQQTWDHREYHHPFDGITLT
                     EFDVATMKLKPETQRTIFEGTEVKLVEGPHIYKINGEYYLFAAEGGTVFTHQEVVARS
                     KTLEAHSFQLAPNTPFITNFDTPNSYLQKQGHGSLVDTPSGEWYYASLTARPWNHATE
                     SATDPRGWSTLGREAAIQKVEFDQDGWPYIVGGHGGKTFVEAPKDAIFTEAPIDHSQH
                     DEFDSSTLDINWNT"
     gene            5739..6377
                     /locus_tag="PXE44_001598"
     CDS             5739..6377
                     /locus_tag="PXE44_001598"
                     /inference="COORDINATES: protein motif:HMM:NF036826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,4-xylosidase"
                     /protein_id="HDL5589098.1"
                     /translation="MPFSKEMGEVGNGVLKLIGRGSLANTHDLSLIARRWQAFYFDAE
                     TKVKFTPFSYQSMAGLTNYYNHSHWSWIFITKNDQGQQVIEVAENKGGLRNGQYTSYL
                     KDKAIVIPDGTEYVWFKTKVRKETYRYEYSFDGKQWHTIPVELDAAVLSDDYVLRSYG
                     GFFTGAFVGLAAVDYSSYESTAEFSYFDYKELGDKQLTDGSFSWEKSESCFG"
     gene            6396..7376
                     /locus_tag="PXE44_001599"
     CDS             6396..7376
                     /locus_tag="PXE44_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL5589099.1"
                     /translation="MKQRRFGKTNKMVSEIGLGTWQLGTKWGEPFNHQEAMAILETAY
                     EQGINFIDTADVYNNGQSEKAIGDILKKYPDFFYVVTKCGRALNPHTAEMYTPQAIEK
                     FVDGSLSHLGTEKLDMILLHCPPTLVYRNDEIFTKLEQLKAGGKLIDYGVSIETAEEG
                     LLAMEYDIAAMEVIFNMFRLKPLEQLFPTAQQKNVGIIARVLLASGLLTGRYNACTQF
                     GKDDHRTFNRNGEAFDKGETFSGVDYQLGLQAVEELKALFGTEDLIPYALRWVLMNEA
                     VSTVIPGASKVSQVITNAAVDNFPPLTENEMQKVEDIYNRLIRPSVHHLW"
     gene            complement(7438..8778)
                     /locus_tag="PXE44_001600"
     CDS             complement(7438..8778)
                     /locus_tag="PXE44_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5589100.1"
                     /translation="MFSFLKASPPAEQKVEASRVDAEYRKLRWQVFAGVFIGYAAYYL
                     IRKNFSLAMPYLIDEYGFTKADLGTVGVALSLAYGFSKFIMGNVSDRSNPKYFITIGL
                     LGSAIVSLVFGLVPGVLSSIPIMIILAALNGWFQGMGYPPGAKTMTNWFSTSERGVWW
                     SWWNVSHNLGGGLIGPLAILGLSIFGVWQSLFYLPALIAIVLAFVMFYLMRDTPESQG
                     LPPIDEYKGEKVVEKVESAHTLSSKDIFYKYILNNKFLWAIAIANVFVYFIRYGIIDW
                     APTYLKEVKHFSVDKQSWAYFLYEYAGIFGMLASGYLSDKVFKGHRAPPMLLFLAGVL
                     IAIVVYWKNPAGNPLIDNICLVAIGFLIYGPVMMIGLQAADLVPRVATGTATGLTGLF
                     GYLLGSASAGYVMGKLVDMFGWDGGFYALILSCFLAFAFIALTLFKKTSKQLEV"
     gene            complement(8968..10041)
                     /gene="glpQ"
                     /locus_tag="PXE44_001601"
     CDS             complement(8968..10041)
                     /gene="glpQ"
                     /locus_tag="PXE44_001601"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL5589101.1"
                     /translation="MKFKFKTLVAALALSATALGAYAQQSDKLVIAHRGASGYLPEHT
                     LESKALAFGQQADYLEQDLAMTKDNRLIVIHDHFLDGLTDVAKKFPERKRADGRYYVV
                     DFTLDEIKTLEMTENFEVKDGKQAQVYPNRFPMWQSHFTIHTLEEELEFIQGLEKSTG
                     KKIGIYPEIKAPWLHHKEGKDIALETLKVLKKYGYDSKTSPIYLQTFDFNELKRIKTE
                     LLPKLGMDVKLVQLVAYTDWHETEEKDKNGNWVNYDYDWMFKDGAMAEVAKYADGVGP
                     GWYMLVDKEKSKVGNIVYTPLVKELAKYKMELHPYTVRKDALPDFFQNVDEMYDALLN
                     KAGATGVFTDFPDTGVEFLKKQK"
     gene            complement(10111..11556)
                     /gene="glpT"
                     /locus_tag="PXE44_001602"
     CDS             complement(10111..11556)
                     /gene="glpT"
                     /locus_tag="PXE44_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDL5589102.1"
                     /translation="MFGPFKPAPHIAELPADKVDARYKFLRWQVFAGIFFGYAAYYFV
                     RANFDLAQKGLIEAGMYTKTDLGVIGTAAGLAYGLSKFVMAGMSDRSNPKVFLPTGLL
                     LSGLCMTLMGLMPWATSSILVMWVMIFLNGWFQGMGWPPCGRTMVHWWSKSERGTIVS
                     IWNTAHNLGGMVPGAMVLLASALYFSEYGVEATAKDVWQQALYFPGIAAMIAAIPIYL
                     VMKDTPQSCGLPPVEKWRNDYPDDYNEKTYEHDLSTKEIFVTYVLKNKLLWYIAIANV
                     FVYLIRYGVLKWSPVYLGEVKHFNIKGTAWAYTIYELAAIPGTLLCGWVSDKLFKGKR
                     GLTGFIFMILTTAAVVAFWLNPATPESEIAQYAGHAWYENPYQLMDFIWMTTIGFLIY
                     GPVMLIGLHALELAPKKAAGTAAGFTGLFGYLGGTVAASAVVGWAADKFGWDGGFYVM
                     IGGGVLAILLTFIVMVEEGKHKAKLEDHYGK"
BASE COUNT         3403 a         2464 c         2510 g         3366 t
ORIGIN      
        1 agtgtcgcat ttagatctta acctctctaa aaatctattt cccagatgat ccctttttac
       61 gctaaaatat gcgttcattt ttcagcagaa tttgtaagat agtagaagag atatgattta
      121 caaccaaatt tacgatgtga tcgtggtcgg cggtggtcac gccggcacag aagcggccct
      181 agcaccggca cgaatgggct taaaaaccct gattttaacc cacaatgttg ataccttagg
      241 gcaaatgtcg tgcaacccgg ctatcggagg gatcggcaaa ggccatctgg ttaaagagat
      301 cgatgcgatg ggcggtttaa tggcaaaagc aacagacgtt gccgggatcc aattccgtac
      361 cctaaacaac tcaaaagggc ctgcggtgcg tgccacccgt gcccaagctg accgtgtgct
      421 ttaccgtaat gcggtacgca ctgcgttaga aaatcaacca aatttagata ttttccagca
      481 agaagtagtg gatattttgg tggaaaacaa ccgtgcagtg ggtgcagtta ccaaaatggg
      541 cttaacgttt aaagcccgtg cggtagtttt aaccgctggt acgttcttag cgggtaagat
      601 ccacattggg ttggataact atgcaggtgg tcgtgccggc gatcctgctg caacaatgtt
      661 ggcagatcgt ctgcgtgatc tgaatttgcg tgttgatcgc cttaaaacag gtacaccacc
      721 tcgcctcgat gcccgtacca tcaactttga cgtacttgcc aaacagcacg gcgatgaggt
      781 gttacctgtc ttctctttta tgggcgatgt ctcacagcac ccacgccaaa ttccgtgcta
      841 catcacccac actaacgatc aaacccacga tttaattcgc aacagtcttg atcgcagccc
      901 gatgtacacc ggtattatcg aaggggttgg tccacgttac tgcccgtcta ttgaagataa
      961 agtaatgcgt tttgccgatc gtaatagcca ccagatctac cttgaaccgg aaggcttaaa
     1021 tacgattgag gtttacccga acgggatctc caccagcttg ccgtttgatg tgcaaatggg
     1081 gatcgtcaac tcaatgaaag gcttggaaaa cacccgcatc atcaaaccgg gctatgcgat
     1141 tgagtacgac tacttcgacc cgcgtgattt aaaaccaacc cttgaaacca aagcgattga
     1201 cggtttattc tttgccggac aaattaacgg cacgaccggt tacgaagagg caggtgcgca
     1261 aggtttgctg gcagggatta acgcagcgtt acaggttcaa ggtaaagagg cgtggtatcc
     1321 aacccgtgat caagcctaca ccggcgtgtt ggtggacgac ctctgtacat taggcaccaa
     1381 agagccatac cgtgtgttta cctctcgtgc cgaataccgt ctgttgctgc gtgaagacaa
     1441 tgcggacatt cgtttaacac caaaagccca cgaactagga ttaattgacg aggcacgttg
     1501 ggtgcgtttc aaccaaaaaa tggaagcgat tgagcaagaa agagctcgtt tgaaatccac
     1561 ttgggcacat ttgcaaatgc caaatctgga cgaattgaac gcgttattgg ctaatccttt
     1621 agcccgtgaa gcaagcggtg aagacttaat ccgccgtcct gaaatgagtt acgaaaagct
     1681 gacccaaatc gccctatttg cccctgctat tgaagacaag caagtggcag aacaggtgga
     1741 aattgcgatc aaatatcagg gctatattga gcatcaatac aacgaaatcg aacgccataa
     1801 acgccacgaa aacaccctga ttccggtaga gtttgactac gacaaagtcg aaagcctctc
     1861 caacgaagtg cgagctaaat tgatgcaaca ccgccctgtc tctatcgggc aggcaagccg
     1921 catttccggc atcacaccgg cagcgatttc cattctgttg gtgaatttga agaagcaagg
     1981 aatgttgaaa cggggtgagc tgtaaatact acaagcggtt ggttttgcag aaatttttgc
     2041 aaaattgacc gcttgtattc tttggagaag gaaatacccg aactaccgcc gtatttttaa
     2101 tgatatatct gcgtaaactc ggtttttcgc cgttcgatca ccaaatgttt gcccaacctt
     2161 cgtggtattt ctgcaatgca cttgttcata ccaattatga aaatctgcga caaggtgtat
     2221 ttgtgactaa agcgtattta gtgatgtttc ttgaaaattt attgtttgaa aaaagtcatt
     2281 cgttaagtaa tcgggcattg cgtattaatg aaaataatga ttaaggaatt tgcaaaatgt
     2341 tagctaaatt aaaccgcttg cttgctcaag ccgaaataaa tttgaccgat caacaaaaag
     2401 agcagttagt tggttttgtg agattattgg ataaatggaa taaggcgtat aacctcactt
     2461 ccgttcgcaa ccctgatgaa atgttggtta aacatatttt ggacagcctt gttgttagtc
     2521 gtcatttaca aggcgaaacg tttattgatg tcggcacggg gccggggcta ccgggcattc
     2581 cgctggcgat tgctaaccct gataaacagt tcgtgctact agacagctta ggcaaacgca
     2641 tcacttttat caaaaatgcg ttgcgtgagc ttggtattaa aaacgttacc ccagtgcaaa
     2701 gtcgagtgga ggaatacact gaaagccagt ttgacggcgt attaagccga gcctttgcct
     2761 cgcttaatga tatgatcgac tggtgttatc atttaccgac tgaaaacggt aaattttatg
     2821 cattaaaagg cgtgtatgat gagcaggaaa taattgaaat caaacaaccg attcagctag
     2881 tggaagtgat taagcttgaa gtacctgagt tggtaggaga acggcattta gtagtgttga
     2941 aaaagcccta atacattgga agtttaatga tacaagcggt cgaatttgta aatttttttg
     3001 caaatctgcc tgcttgtgtt aactattttt cataaagcat acctggttaa taaggtgtgc
     3061 tttttttata acctattatt tcatctgatt tatttctatt ttgtgatgtg cttcacaaaa
     3121 acaaaaaaac ataatgtagt aaaaaatatt cgtaattgaa gcccttattt ctttacctga
     3181 aaataagcga acttaattat ataacgagga ggttcgtaat gaacagggta acaaataagg
     3241 cagatgagtt tgctaaactc agtacgtttg aacgtattgc ttatggtagt ggcgacttag
     3301 cccaaaattt agtttttggt acagttggtg gtttcttact cacttactta actactgtaa
     3361 acggcatttc tgccgtagca ggagcaacga ttttcttaat tgtaaaatgg ggaaatgtgt
     3421 tttgggatcc gtttgtcggt gcctttattg acaaaagcaa gccctcagca cagggtaaat
     3481 accgcccgta tttactgaaa tttgggattc ctcttgtggt tttagcttca ctactatttc
     3541 ttcctcttga atcagtacgt ggctcagtga tttatgcttt cctttcttat tttgccactg
     3601 cgatggttta ttcttttgtt aatattcctt atggctcgct ttctgcttct cttactcgag
     3661 acacagacga aattgctaaa ttaaccacaa cccgtatgac aatggcaaat atcgctaatt
     3721 tgtttgttta taccttattc ccactttttg tgcagttact tgcaccacat gccgaattaa
     3781 aaaacaccgg ctttttcggt attgagctct atttaggtga ttatatgtct gctgctgcag
     3841 gccctgcttg gtttaaggtt tatgcggtct atatggtaat tggatttgtg gcgttaatgc
     3901 tctcttattt tggtactaaa gagcggatta ttccaacagc ggaagaaaca gataaagtca
     3961 agtattcaga tttagttaat gaactcaaac gcaatcgtcc attacagatt ttagggttat
     4021 tcttcttaat aggttttact tttatgttct tcggaaatac ggtgtggcct ttttacctaa
     4081 aatataatat tgggcattct gagtggattg cctccgtcgg tttattaggc tctatccctg
     4141 ggattttcct tgtattctta tggccgaaac tacgtcgcac tatcggtaaa aagaactttt
     4201 tctatctatt tttagggatt ttcgttctag ggcaactttg tctatgggta tggcaatttg
     4261 actccttcaa atactctcca accttaggct atatcggtcg tttcttacaa caatggggat
     4321 taacttctgc taccggtttt atgtgggcgt tagtgcctga ggttgttacc tttggagagt
     4381 ttaagtcgaa aaaacgtgta gcaggtatta ttaatgcgtt aatgggcttg ttctttaaaa
     4441 tcggcttggc gttaggcggt attattccgg gttatattaa tgcggtttat ggttttaacg
     4501 gctcgctttt ggctcaaacc gaagaggctt tatttggtat caatctttca atgatttggg
     4561 ctccaattat tttagcgatt attgcaggat ttgtgatgag tcgctatccg ttaagcgata
     4621 ctgatattga tcatatcaac gaaaaaatta ctgcttattc caaataattt gagtatagga
     4681 gaatagatta tgcaaattca aaatccagtt ttacccggtt tcaatccaga tccatctatt
     4741 gtgcgtgtgg gtgatactta ctacattgca acttctactt ttgaatggtt tccgggagtg
     4801 agaattcacg aatcgaaaga tttagtgcat tggaatttag ttaaaaatgt cttagataca
     4861 accacgttgt tagatatgcg aggcaatccc tcttcaggtg gtatctgggc tcctgatctt
     4921 tcttatgcgg aaggtaaatt ttggttggtt tataccgatg taaaaattac agacggtgca
     4981 ttcaaagata tgactaacta tctaactact gccaaagaaa ttcaaggtcc ttggtcagct
     5041 ccgattaaat tgaatggtat tgggtttgat gcttcgttgt ttcacgatga tgatgggcga
     5101 aaatatttag tgcaacagac ttgggatcac cgtgaatatc atcacccctt tgacggtatt
     5161 acgttaacgg aatttgatgt tgccacaatg aaactcaaac ctgaaaccca gcgtacgatt
     5221 tttgaaggta cagaagtgaa attggtggaa ggtccacata tttataaaat taatggcgaa
     5281 tattatctgt ttgcggcgga aggcggcaca gtatttacgc atcaagaagt agtggctcgt
     5341 tctaaaacat tggaggctca ctctttccaa ctggctccaa atacaccgtt tattactaat
     5401 tttgatacac ctaattctta cctacaaaaa caagggcacg gttcattagt tgatacccca
     5461 agtggtgaat ggtattacgc ttccctcaca gcaagacctt ggaatcatgc aacagaatcc
     5521 gcaaccgatc ctcgtggttg gtcaacatta gggcgtgaag ccgccattca aaaagtagaa
     5581 tttgatcaag acggctggcc ttatattgtg ggtggacacg gtggaaaaac ctttgtagaa
     5641 gccccaaaag atgcaatttt taccgaagct cctattgatc attctcaaca cgatgagttt
     5701 gatagctcaa cgttagatat taactggaac acttaagagt gccttttagt aaagagatgg
     5761 gcgaagttgg taatggtgtt ttaaaattga ttgggcgagg ctctcttgca aatacacacg
     5821 atttatcgct aatcgcacgc cgttggcaag ccttctattt tgatgcggaa accaaagtga
     5881 aatttacacc gttttcttat caatctatgg caggtttaac taactattac aatcactcac
     5941 actggtcttg gattttcatc accaaaaatg atcaaggaca acaggtgatt gaagttgcag
     6001 aaaataaagg tggactcaga aatgggcaat atacttctta ccttaaagat aaagcaattg
     6061 tcattcctga tggaacggag tatgtttggt ttaagactaa agttcggaaa gaaacttacc
     6121 gttatgaata ttcattcgat ggcaaacaat ggcacactat tccggttgaa ttagatgcgg
     6181 cagtgctttc agatgattat gtgttacgca gttatggcgg tttcttcaca ggagcttttg
     6241 tcgggctggc tgcagtggat tactccagtt atgaatctac agcagaattt agctactttg
     6301 actataaaga gttaggcgat aagcaactta cagacggctc attctcttgg gaaaaatctg
     6361 aaagttgctt tggctaaaat gaggagaata tcacaatgaa acaacgcaga tttggtaaaa
     6421 ccaacaaaat ggtgtcagaa attgggttag gcacttggca gcttgggacg aaatggggtg
     6481 aaccgttcaa tcatcaagag gcgatggcaa ttttagaaac cgcttatgag caaggcatca
     6541 attttattga taccgctgat gtgtataaca acgggcaaag cgagaaagcc attggtgata
     6601 ttttgaaaaa ataccctgac tttttctatg tcgttaccaa atgtggacgt gcgttaaacc
     6661 cgcatactgc tgaaatgtac acgccacagg caatcgaaaa atttgttgat ggtagtttaa
     6721 gccatcttgg cacggaaaag ttggatatga ttttgctcca ctgcccgcca acgttggttt
     6781 atcgcaatga tgagattttt acgaaattgg aacagcttaa agccggcgga aaattaatag
     6841 actacggtgt gagtatcgaa acggcagagg aaggcttgtt ggcaatggag tatgacattg
     6901 cggcaatgga agttatcttc aatatgttcc gcttaaaacc attggagcaa ctcttcccaa
     6961 ccgctcagca aaaaaatgtg ggtattatcg cccgtgtact gttggcaagt ggtttgctta
     7021 caggcagata taatgcctgc acccaatttg gcaaagacga ccaccgcaca tttaaccgca
     7081 atggtgaagc cttcgataaa ggcgaaacct tttcgggtgt ggattatcaa ctcggtttac
     7141 aagcggtgga agaattgaaa gccctattcg gcacggaaga tttaattcct tacgccctac
     7201 gttgggtatt gatgaatgaa gctgtgagta cagtaatccc gggagcaagt aaggtttcac
     7261 aagtaattac taacgcagcg gtggataatt tccctccact cactgaaaat gaaatgcaaa
     7321 aagtggaaga tatttacaac cgcttaattc gcccaagtgt gcatcatttg tggtaattta
     7381 tccaacgcta taaaaaatgg aactctaact tgagttccat ttttgtcaat tttagcctta
     7441 aacctctaac tgtttagagg ttttcttaaa cagagtcaat gcaataaacg caaaggctaa
     7501 gaaacaggaa aggatcaggg catagaaacc accgtcccag ccgaacatat caaccagttt
     7561 acccatcaca taacctgcac ttgcagagcc taacaagtag ccgaataaac cggttaaacc
     7621 tgttgctgta ccggtggcaa cacgaggcac taaatccgct gcttgtaaac cgatcatcat
     7681 caccggcccg tagattaaga aaccaatcgc cactaagcag atgttgtcga ttaacgggtt
     7741 gccggctggg tttttccagt acaccacaat cgcaattaac acaccagcaa ggaataacaa
     7801 catcggcggc gcacggtgac ctttgaatac tttgtcgctt aaataaccgc ttgcaagcat
     7861 accgaagata ccggcatatt catacaagaa gtacgcccag ctctgtttat ccacagagaa
     7921 atgtttcact tcttttaagt aagtcggtgc ccagtcgata atgccgtagc gaatgaaata
     7981 gacaaacacg ttggcaatcg caattgccca caggaatttg ttgtttaaaa tatatttgta
     8041 gaaaatgtct ttggaagaga gtgtgtgggc agactccact ttttccacca ctttctcgcc
     8101 tttgtactca tcaatcggcg gtaagccttg agattccggc gtatcacgca tcaaatagaa
     8161 catcacaaac gccagtacaa tcgcaatcaa cgccggcaga tagaacaggg attgccacac
     8221 gccaaaaata gataagccta aaatcgccaa cggcccgatt aaaccgccgc ccaagttgtg
     8281 cgacacgttc caccagctcc accacacgcc acgttcagag gttgagaacc agttggtcat
     8341 cgttttcgcc cccggcggat agcccattcc ttggaaccag ccgtttaacg ccgccaaaat
     8401 gatcataatt ggaatagacg aaagcacgcc cggtactaaa ccgaacacca gactcacaat
     8461 cgctgaaccg agcaagccga tagtgataaa gtatttcggg ttagaacggt cggaaacgtt
     8521 acccataata aatttactga agccgtaagc gagtgataat gccacgccca ccgtacctaa
     8581 gtctgctttg gtgaagccat attcatcaat caaatacggc attgccaacg agaagttttt
     8641 acgaattaaa taatatgccg catagccgat aaagacgcct gcaaacactt gccaacgtaa
     8701 tttgcgatat tccgcatcaa ctctgctggc ttccactttt tgttctgctg gtggagaggc
     8761 ttttagaaag gaaaacattt tactactcct ttaagaatga aatcctatgg ctactataat
     8821 ctttcatttg gagcagaaaa agtggatttt tgaaaatttg tgagcaagat taaaattatt
     8881 ttgagcgaaa acgaaaagcg gttgaatttc cttctttttt tgcaaaaaat ttgagaaatc
     8941 taaccgcttg tttcacttaa aaatcagcta tttctgcttt ttcaaaaatt ccacgcccgt
     9001 atcagggaag tcagtaaaga cgcctgttgc acccgcttta ttcagcaggg catcgtacat
     9061 ttcatcgaca ttttggaaaa agtccggcag agcatctttg cgcacggtgt atgggtgaag
     9121 ttccattttg tacttcgcca actctttcac taatggcgtg taaacaatgt tgccgacttt
     9181 agatttttct ttatccacca acatatacca gcccgggccg acaccatcgg catattttgc
     9241 cacttctgcc atcgcaccgt ctttgaacat ccagtcgtag tcgtaattaa cccagttgcc
     9301 gtttttatct ttctcttcgg tttcgtgcca atcggtgtag gcaactaact gcaccaattt
     9361 tacgtccata cccagtttcg gtagtaattc ggttttgatc cgtttcagct cgttgaaatc
     9421 gaaggtttgc aggtaaattg gtgaggtttt gctgtcgtag ccatattttt tcaacacttt
     9481 cagggtttcg agtgcaatgt ctttaccctc tttatggtgc aaccaagggg ctttaatttc
     9541 aggatagatc ccaatttttt tgccggttga tttctctaat ccttgaataa attccagctc
     9601 ttcttccaaa gtgtgaatgg tgaaatggga ctgccacatc gggaagcggt tcgggtaaac
     9661 ttgtgcctgc ttgccgtctt tcacctcaaa attctcggtc atttcaaggg ttttaatctc
     9721 atcaagggtg aaatccacca cataataacg accgtctgct cgtttacgct cagggaattt
     9781 tttcgccaca tcagttaaac cgtctaaaaa gtggtcgtga atcacaatca ggcggttatc
     9841 tttggtcata gcaagatctt gctctaaata gtccgcttgt tgcccgaatg ctagagcttt
     9901 agattcaagc gtatgctcag gtaaataacc gcttgcacca cggtgggcaa tcactaattt
     9961 atcagattgt tgtgcatagg cacccaatgc tgtcgcggac aatgctaatg cagcaaccaa
    10021 tgttttgaat ttaaatttca tagaattacc tcatataatg taaaaataaa aggaagaaca
    10081 aaaatgtcct tccttttgat aagttattta ttatttacca tagtgatctt ctaattttgc
    10141 tttgtgcttg ccttcttcaa ccatcacaat gaaggttaag agaatggcta atacaccacc
    10201 accgatcatc acgtagaaac cgccgtccca gccaaattta tctgctgccc agccaactac
    10261 tgcagaggct gccactgtac cacctaagta accgaataaa ccggtgaaac ctgctgctgt
    10321 acctgctgct tttttcggtg caagttcaag ggcgtgtaag ccaatcaaca ttaccgggcc
    10381 gtagattaag aaaccgatag tggtcatcca aatgaagtcc attaattgat atgggttttc
    10441 ataccaagca tggcctgcat attgtgcaat ttcagattcc ggtgtcgcag ggttgagcca
    10501 gaacgcaacc actgctgcgg tggtaagaat cataaagatg aaaccggtta aaccacgttt
    10561 gcctttgaac agtttgtcag agacccaacc acagagtaat gtacctggaa tcgcagctaa
    10621 ttcataaatg gtatatgccc atgctgtacc tttaatgttg aagtgtttta cttcgcctaa
    10681 gtacaccggc gaccatttca atacgccata gcggattaag taaacgaaga cattcgcaat
    10741 ggcaatgtac cataacagtt tgtttttcag cacataagtc acgaaaatct ctttggtgct
    10801 taaatcgtgt tcataggttt tttcgttata gtcgtcaggg tagtcgttac gccatttttc
    10861 aactggcggt aaaccgcaag attgtggagt atctttcatc acaaggtaga ttggaatagc
    10921 tgcgatcatt gctgcaattc ccgggaaata aagggcttgt tgccatacat cttttgccgt
    10981 tgcttctaca ccatactcac tgaaatagag cgcacttgct aatagcacca tcgcccccgg
    11041 caccataccg cctaagttgt gagcggtatt ccagatagaa acgatagtac cacgctcaga
    11101 tttagaccac cagtgaacca ttgttcgtcc acacggtggc caacccatac cttgaaacca
    11161 gccgtttaag aagatcatta cccacattac taaaatgctt gatgttgccc aaggcattaa
    11221 gcccataagg gtcatacata gacctgaaag taacaatccg gtcggtaaaa atactttcgg
    11281 gttagagcgg tcggacattc ctgccatcac gaattttgat aaaccgtagg ccaggccggc
    11341 agctgtacca atcacaccga gatcggtttt tgtatacata ccagcttcaa tcaagccttt
    11401 ttgtgctaag tcgaagtttg cacgcacaaa atagtaagcg gcatagccga agaaaattcc
    11461 tgcaaacact tgccaacgca agaatttata tcttgcatcg actttgtccg ccggtaattc
    11521 cgcaatgtgc ggagcgggtt taaatggacc aaacatagta ttctccaata ttacttagta
    11581 aaacctctga gtaattcctt tttaggaaat tagggaagca ataataaatg aaaataaaaa
    11641 atgacaaagt tattttttat aaaaatgtga ttcatttcac aaaaaattta ccttaaacca
    11701 caaaaaatga ttgaaaaaga aaatcaaaca tcagtatctc tag
//
LOCUS       DANLZC010000078        11700 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.078, whole genome shotgun sequence.
ACCESSION   DANLZC010000078 DANLZC010000000
VERSION     DANLZC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11700
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.078"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            20..277
                     /locus_tag="PXE44_001603"
     CDS             20..277
                     /locus_tag="PXE44_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5589103.1"
                     /translation="MKNLRLSIHSSEHIWLRELFLKRRLELGLTQRTLGEKMGVLYSF
                     IGKVETGDRRLDIFEFIAYCKGLDLDPLSVLQEIEQQFPHK"
     gene            complement(324..944)
                     /locus_tag="PXE44_001604"
     CDS             complement(324..944)
                     /locus_tag="PXE44_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDL5589104.1"
                     /translation="MKLWYSTTSPFARKALAVIKHHQLDDKVEMLRVTKAMDPNSPHN
                     QDNPLGRIPALQRNCGRWLFGSLLISEYLDQKGNNTPLLPKEGKPRWAVLALHNLADG
                     IMENTMPTLLQERMARPENEWWVSRHEQLMERNIRSFRQLEEALQEFGTELNLGTLTA
                     VALIDWWAFRLDKIGYDLAKNYPNLTAWAEEMNNKYAILAETKPSM"
     gene            complement(946..1599)
                     /locus_tag="PXE44_001605"
     CDS             complement(946..1599)
                     /locus_tag="PXE44_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HDL5589105.1"
                     /translation="MTPTLPICYQHRDFIIINKPEGLSVHKDEAEIGLTELVARQLNV
                     KQVWLVHRLDKITSGLLILALNKEAAARFYHLFEQHKIYKTYWALSDKKPKKKQGRIV
                     GDMQKSRNGAWKLCHSKENPAVTQFISHSVEDSLRHFILQPKTGKTHQLRVAMKSLGS
                     PILGDKLYSGSQADRVYLHAYQLDFEYENEKISVQALPTSGHFWQKICKSNHIKHGE"
     gene            1648..2307
                     /locus_tag="PXE44_001606"
     CDS             1648..2307
                     /locus_tag="PXE44_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL5589106.1"
                     /translation="MAVTIYLVRHGRTVWNLEGRLQGSGDSALVEEGIIGAKKTGIAL
                     KHIPFTAAYSSMQKRAQDTANYILAENERSNIPHFHHKGLNEFDFGSWEGMKSVDLQE
                     NDEYWVMKRTPAEYLAKANGGERFEQLYQRVTQAFNQIAELHQNDGKVLIVSHGMTLT
                     LLTAVLKGIAWQDFRNEEKHSFVFNTAITKVEVENGKVTVLEFNNTDHLDNVPTSQYK
                     R"
     gene            complement(2344..3390)
                     /locus_tag="PXE44_001607"
     CDS             complement(2344..3390)
                     /locus_tag="PXE44_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDL5589107.1"
                     /translation="MSKQIFNEEELKTTEHFEPKQEFDLENAIIEEEEKAVEAELIIE
                     ESLKPSRFWIRVLLATLALFGVAVIAQSIQWLIDTFQQREWIYFAFSIVFFIVSLLGV
                     GAIISEWRKLVYLRNHQKNQQMSNQLLLEQLPSNDGEQAVKFCENIANQLNHLPSIAQ
                     AEQLWKSELNEAYNAKEVLYLFSENVLKPIDKQVKQMISRNAAENAVIVAVSPLAIVD
                     VLLMAARNIALVNKITKAYGMELGYISRLKLFKMVLKNMVFAGATEIATDVGMDFFSQ
                     NLTAKLSLRAAQGIGVGLLTARLGIKAMEFCRPVAFQANEKPRISAIRQELLTSVKNT
                     VFAKTETKEKVFSD"
     gene            complement(3390..4343)
                     /gene="dusC"
                     /locus_tag="PXE44_001608"
     CDS             complement(3390..4343)
                     /gene="dusC"
                     /locus_tag="PXE44_001608"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDL5589108.1"
                     /translation="MNPIPNVILAPMQGVLDPFVRNLLTSVNDYDLCISEFVRVVDQR
                     LPKKTFYRLAPELYQGGLTDSGTPVRVQLLGQHPQWLAENAALAIELGSHGVDLNCGC
                     PSKTVNGSNGGASLLKDPELIYRATKAMREAVPSDKIVSVKVRLGWDSVSQCFEIADA
                     VEQGGANEITVHGRTKVDGYRAERINWQAIGEIQQRLSIPVIANGEIWDFESAKNCQK
                     ITACKALMMGRGALNTPNLSKVVKYNVPKMAWNEVLQLLFKYVQMENQFDTGFYHVAR
                     IKQWLRYLDKEYPQAVELFDILKTEHGYDGLKAHIEKAVEE"
     gene            complement(4421..5047)
                     /locus_tag="PXE44_001609"
     CDS             complement(4421..5047)
                     /locus_tag="PXE44_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDL5589109.1"
                     /translation="MDSRSHIIPLFLAIVITVIAVWSGLNPTDRAVWYAEVIPIFIVF
                     ALLIITYPKFQFSGLAYFLMSLWLIMHLIGAKYTFANVPFEWANQFLTPILGEDRNHF
                     DRVAHYIIGFYSFPMAEWLLRKRKCGMGVAFFFSLFFIMSVAAAYEIIEWQYAVIEGG
                     NAGVEFLGSQGDIWDAQKDMLADTLGALTALMIYLIARPDLRIHDRHF"
     gene            complement(5047..5904)
                     /gene="djlA"
                     /locus_tag="PXE44_001610"
     CDS             complement(5047..5904)
                     /gene="djlA"
                     /locus_tag="PXE44_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDL5589110.1"
                     /translation="MQFIGKIVGFFLGYQLFHGLFGGILGAFIGHLADKKLYELGSVR
                     SSLFGKNLTRQSLFTQTTFAVLGHIAKAKGRVTEADIELARALMARLKLDSAAQQLAQ
                     NAFTLGKEPDFPLRQVIQEFREACGARTDLLRFFVEVQMQAALQDGQLDVNEQQILFT
                     IAETMGMSRFQFEQMIAMIMAAQQFRNGGYYQQNGSYHQQSQGGYQGSYQQPAQPSIE
                     AAYTVLGVTAQDDQNTVKRAYRKLMNEHHPDKLAAKGLPDEMMELAKEKAQQIQAAYD
                     LICKVKEWK"
     gene            complement(5952..6662)
                     /locus_tag="PXE44_001611"
     CDS             complement(5952..6662)
                     /locus_tag="PXE44_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5589111.1"
                     /translation="MWKNRQLFFGYAENRLLLLSRSITGLLGILCGVYIIDHLVLSDV
                     DMIGKLTSFILIILSAIFLKERASLLQWILCLIAFIGALFIIKPAVDIRFMPYLVGVI
                     GSVFAAIAYLCLRLLAKTKKVESPNTIVFFFSAFSTLILLPFVAIDYVVLTNLQTIYL
                     VLAGVTSAVGQFCVTTAYKYAAAKDISIYSYASVLFSAILGLLFFEQYPDMLSWLGYA
                     IIFVSSWLMFVLTKKQSK"
     gene            complement(6898..7383)
                     /locus_tag="PXE44_001612"
     CDS             complement(6898..7383)
                     /locus_tag="PXE44_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL5589112.1"
                     /translation="MKKFAKVLMVAVPAFALAACSSSSDDAAANAAANAAANAAANAA
                     GEFGGLTAEQLKAQYNTVYFGFDSYAVEGEYRQLLDAHAQLLAKTQGNVTIAGHADER
                     GTPEYNIALGQRRADAVKGYLAAKGANNTSTVSYGEEKPAVLGHSEADYAKNRRAVLE
                     Y"
     gene            complement(7410..8696)
                     /gene="tolB"
                     /locus_tag="PXE44_001613"
     CDS             complement(7410..8696)
                     /gene="tolB"
                     /locus_tag="PXE44_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDL5589113.1"
                     /translation="MKLFSRISGVIGIFSALLTTQVVADSDVRISIDEGVSMAQPIAV
                     VPFKASGVPADVAQIISDDLRNSGKFTPLERSQMPAQPGSAAEVVADQWSAIGIDNIV
                     VGQVSSTGGGYNVAYQLIDTLGTSGTAGGVIAQGSFIVPAAQIRQGAHTVSDEVFEKL
                     TQIRGAFRTKIAYVVQRGVSSYELRVSDYDGFNAFTITTSKEPLMSPEWSPDGSRLAY
                     VTFENKKAQVVVHDIRSGARRVVAALKGHNGAPSFSPDGSRIAFASNQDGELNIYVVG
                     SGGGTPSKLTANAGNNTEPSWSPDGSTIYFTSDRAGSPQVYRMSSSGGGASPMGGSGS
                     YNAKVSSDGKNLIMIAGDRVVKRDLASGGTEVLSSTFLDESPSISPNGIMVIYSSTKG
                     TSKVLQLVSADGRFKANLPGAGGHYKFPAWSPYLTK"
     gene            complement(8712..9935)
                     /gene="tolA"
                     /locus_tag="PXE44_001614"
     CDS             complement(8712..9935)
                     /gene="tolA"
                     /locus_tag="PXE44_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDL5589114.1"
                     /translation="MKSQQDRLELAVIVSILLHILLIGLLLLGSLFTKTELAAAGGSG
                     GDSDSFEAVMVDTGQVAAEYGQLLAEKKGSKASKPKVEPKEEAVEEDVEDKPTPEEIQ
                     KQKQAELAAIQEKQRQQEIERQQKLQEQQKQEQAKQEELKKQQAEEATRKKAAEAAKL
                     KADAEAKRLEAAAKQAEEEKKAKEALEKKLEQQKKAEAEKKLKEQKELKEQQAKEAKE
                     AKEKAEKEAKAKAEKEAKEKAEKEAKAKAEKEAKEKAEKEAKAKAAAEAKAAQAKNNK
                     ALDDFLNSGDIGGGSSKGGNKNSAGSQGSGGTSGLGQGTNVDGNAYGQRIKKLIQSRY
                     RVDPSFAGKQCDVKIFLSRDGTITNYQVISGDKAVCDAAVSAIVATRKVPPAPSDAVY
                     NMFKSPTLDFSLKVK"
     gene            complement(9949..10386)
                     /gene="tolR"
                     /locus_tag="PXE44_001615"
     CDS             complement(9949..10386)
                     /gene="tolR"
                     /locus_tag="PXE44_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDL5589115.1"
                     /translation="MSRRLKRNDIKSEINIVPFLDVLLVLLLIFMATAPIISQSVEVN
                     LPEDTHSQSVSNEDKTPVILEVAGVGEYKLKIDGNYVQSNGQENINESEVVAYAGEQF
                     QKDNNTLFLVAGDKVVPYEEIMKGITLLKDAGVKSVGLMTEGK"
     gene            complement(10528..11208)
                     /gene="tolQ"
                     /locus_tag="PXE44_001616"
     CDS             complement(10528..11208)
                     /gene="tolQ"
                     /locus_tag="PXE44_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HDL5589116.1"
                     /translation="MQTEFSLLSLFLEASIVVKIVMLILIVFSILSWAVIIQRSKILT
                     KAKKDSLAFEDRFWSGEDLNRLHQGLENRRDALNGSEQIFYVGFKEFTRLQQANPDAP
                     ESIIQGSTRAMNLALNREMESLENYIPFLGTVGSISPYIGLFGTVWGIMHSFMGLSAV
                     KQATLQSVAPGIAEALIATAIGLFAAIPAVMAYNRLNLRLGKVEQGYVNFIDEFTTLL
                     HRQAFSKR"
     gene            complement(11230..11631)
                     /gene="ybgC"
                     /locus_tag="PXE44_001617"
     CDS             complement(11230..11631)
                     /gene="ybgC"
                     /locus_tag="PXE44_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDL5589117.1"
                     /translation="MDFPIRVYYEDTDAGGVVYHSNYLNFFERARTEFLRQQHFSQQE
                     LFSQSLAFVVKRIEIDYKLPARLDDLLNVETTLSELKKATLIFKQRLWKDGDCLSEAN
                     VTVACVDLIKMKPVAIPDDIRQALQKSYFSI"
BASE COUNT         3376 a         2635 c         2140 g         3549 t
ORIGIN      
        1 tgtacctaat ataggtgcaa tgaaaaatct tagattatca atacattcca gcgagcatat
       61 ttggttaaga gaactattct taaaacgccg tttggaacta gggctaacac aacgcacatt
      121 aggagaaaaa atgggcgttc tttactcatt tatcggaaaa gtagaaacag gagatcgcag
      181 attagatatt tttgaattta tcgcttattg caaagggtta gatttagatc ctttatctgt
      241 attgcaagaa attgaacaac aatttcctca taaataaagg cattgagtta taactcaatg
      301 cctttatttt ctatgtcaaa tctctacatg ctcggcttag tctctgccag aatggcgtat
      361 ttattattca tttcctctgc ccaagcggtt aaatttgggt aattttttgc aagatcgtag
      421 cctatcttat ctaagcggaa tgcccaccag tcaattaatg ctactgcggt taatgtgcct
      481 aaattcagct ctgtgccaaa ctcttgcaat gcctcttcta attgtctgaa cgaacggata
      541 ttgcgttcca ttaattgctc gtggcggctg acccaccatt cattctcggg gcgagccata
      601 cgttcttgta ataaagttgg catcgtattt tccataatgc cgtccgccaa attatgtaat
      661 gccaacactg cccagcgagg tttaccctct ttaggtaaca gtggagtgtt attgcctttt
      721 tggtcaagat attcgctaat taacaaacta ccgaaaagcc aacgtccgca gttgcgttgt
      781 agggcaggaa tacgtcctaa cgggttatct tgattatgtg gcgaatttgg atccattgct
      841 tttgtcaccc gcaacatttc gactttatcg tctaattggt ggtgtttaat caccgcaagg
      901 gctttgcggg cgaaagggct ggtggttgag taccaaagtt tcatactatt ctccgtgttt
      961 gatgtgattg gatttgcaaa ttttttgcca aaaatgaccg cttgtcggca aggcttgcac
     1021 tgagattttc tcattttcgt attcaaaatc aagctgataa gcgtgcaaat aaacacggtc
     1081 ggcttggcta cctgaataga gtttatcacc taaaatcggg ctgcccaaac ttttcattgc
     1141 cactcttaat tggtgagttt tgcctgtttt gggttgcaaa ataaaatgcc tgagcgagtc
     1201 ttcaacagaa tgagaaataa attgcgttac tgccgggttt tccttactgt ggcataattt
     1261 ccacgctcca tttctgcttt tttgcatatc gccaacaatt ctaccctgct ttttcttcgg
     1321 ctttttatcg cttaatgccc aataggtttt gtagatttta tgctgttcaa ataaatggta
     1381 aaaccttgct gccgcctctt tattcaacgc taaaatcagt aaacctgagg tgattttatc
     1441 cagacggtga accagccaaa cctgctttac attcagttgg cgagccacta attccgttaa
     1501 gccgatttca gcctcatctt tatgcacact caagccttcc ggcttattga taataataaa
     1561 atcacgatgt tgataacaaa taggcaatgt tggggtcatt ttgctataat ccttccaatt
     1621 tttgactatt ttagcaagga aattacaatg gctgttacca tttatttagt acgacacggc
     1681 agaaccgtgt ggaatttgga aggcagatta caaggctcgg gcgattccgc tttagtagaa
     1741 gaagggatta tcggagcgaa gaaaaccggc attgcgttaa aacatattcc gtttaccgcc
     1801 gcctattcca gtatgcagaa acgggctcaa gataccgcaa actacatttt agcagaaaat
     1861 gaacgcagta atattccgca ttttcatcat aaaggcttga atgaatttga ttttggcagc
     1921 tgggaaggaa tgaaatctgt cgatttgcaa gaaaatgatg aatattgggt gatgaaacgt
     1981 acaccggcag aatatctggc aaaagcaaac ggtggcgagc gatttgagca actttatcaa
     2041 cgggtgactc aggcgttcaa tcaaatcgcc gagctacacc aaaatgacgg caaagtgctg
     2101 attgtttcac atggtatgac cttaacctta ctcacggctg tgttaaaagg cattgcgtgg
     2161 caagatttcc gcaatgaaga aaaacatagc tttgtgttca atactgccat tacgaaagta
     2221 gaagtagaga atggcaaagt aacggtgctg gaatttaata ataccgacca tttggataat
     2281 gtgccaacaa gccaatacaa acgataatat taagggcgga acattgtccg cccatagcat
     2341 tttctaatca gagaacactt tttctttcgt ttctgtttta gcaaataccg tatttttgac
     2401 cgaagtaagc aactcctgac gaatagctga aattcttggc ttctcattcg cttgaaaagc
     2461 aacaggacga caaaattcca ttgctttaat gccaaggcga gcagtgagta aacccacacc
     2521 aataccttgt gcggcacgga gcgataattt cgccgtaagg ttttgcgaga agaaatccat
     2581 tccaacatca gtagcgattt cggttgcacc ggcgaacacc atatttttca gcaccatttt
     2641 gaacagtttt aagcggctga tataacccaa ttccattcca taggctttgg taattttatt
     2701 cactaaagcg atatttcttg ccgccattaa caacacatca actattgcca gcgggctaac
     2761 cgccacaatc accgcatttt ctgccgcatt gcgggaaatc atctgcttaa cctgtttatc
     2821 tatcggcttt agcacatttt cgctaaacag atatagtacc tctttggcat tataagcctc
     2881 gttcagttcg cttttccaaa gttgctcggc ttgagcaatg ctcggcaagt gattaagctg
     2941 atttgcaata ttttcgcaaa atttgaccgc ttgttcgcca tcattggaag gcagctgttc
     3001 cagcaataat tggttactca tttgttgatt tttctgatga tttcgcaaat acaccaactt
     3061 tcgccattcg gaaataatcg ccccaacgcc caacaagctg acaataaaaa agactatcga
     3121 gaacgcaaaa tagatccact ctctttgctg gaacgtatca atcaaccact gaatactttg
     3181 agcaattacc gccacgccaa acaaggcaag tgtggcaagt agtactcgaa tccaaaagcg
     3241 ggaaggctta aggctttctt caataatcag ctccgcctca actgcttttt cctcttcttc
     3301 aataatcgca ttttcgagat caaactcttg tttaggttca aaatgctcgg ttgtttttag
     3361 ctcttcttca ttaaaaattt gcttactcat tattcctcca ctgccttttc aatatgtgct
     3421 tttagaccat catagccgtg ttcggttttc aagatatcaa ataattctac cgcttgagga
     3481 tattctttat ccaaataacg tagccattgt ttgatgcgag caacgtggta aaaaccggta
     3541 tcaaactgat tttccatctg aacgtactta aacaatagct gtagcacttc attccacgcc
     3601 attttcggaa cattatattt caccactttg cttaaattcg gtgtgtttaa cgccccacgc
     3661 cccatcatta atgccttgca agcggttatt ttttggcaat ttttcgccga ttcaaaatcc
     3721 caaatttcgc cgttggcgat aacaggaata gacagccgtt gttgaatttc gccaatcgcc
     3781 tgccaattaa ttctttctgc tcgataacca tcaactttgg ttcgcccgtg aacggtaatt
     3841 tcattcgctc cgccctgttc taccgcatcg gcaatttcaa aacattggct aacggaatcc
     3901 caacctaatc gcacttttac ggaaacgatt ttatcgcttg gcacagcttc acgcatcgct
     3961 ttggttgcac gataaatcag ttccggatct tttaataacg aagccccacc attgctgcca
     4021 tttaccgttt tagaggggca gccacagttg agatctaccc cgtgtgaacc aagctcaatg
     4081 gcaagagctg cattttctgc cagccactgt gggtgttgcc ctaaaagctg tacccgcact
     4141 ggtgtgccgg aatcggttaa tccgccttga tatagctcag gggctaggcg ataaaaggtt
     4201 ttcttcggta aacgttgatc gaccactcgc acaaattcgg aaatacagag atcgtagtca
     4261 ttcactgaag ttaaaaggtt gcggacaaaa gggtctaaaa ccccttgcat cggggcaaga
     4321 ataacgttag gaattggatt cattggtaaa ataaaaaata caagcggtta ttttttctca
     4381 acattttgca aaaaattggc aagaaacaac cgcttgaagg ttaaaaatgt ctatcgtgaa
     4441 tgcgcaaatc ggggcgagcg atcaggtaaa tcatcagtgc ggttaatgcc ccaagagtat
     4501 ccgcaagcat atctttttga gcgtcccaaa tatccccttg cgagcctaga aattccacgc
     4561 cggcattgcc accttcaatc acggcatatt gccattcaat aatttcgtaa gctgccgcca
     4621 ccgacataat gaaaaacagc gagaagaaaa atgctacgcc cattccgcac ttgcgtttac
     4681 gcagtaacca ttctgccatc gggaagctgt aaaaaccgat aatgtaatgt gctactcgat
     4741 caaaatggtt acgatcttca cctaaaatcg gggttagaaa ttgatttgcc cactcaaacg
     4801 gcacattggc aaaggtatat tttgccccga tcaagtgcat aatcagccaa agcgacatca
     4861 aaaaataagc taacccacta aactgaaatt tagggtaagt gataattaaa agggcaaaaa
     4921 cgataaaaat cggtatcact tccgcatacc aaactgcgcg gtctgtcgga tttaaccctg
     4981 accaaaccgc aatcacggta ataacaatcg ctaaaaatag agggatgata tgcgagcgtg
     5041 aatccattat ttccactctt tcactttaca aattaaatcg tacgccgctt gaatttgttg
     5101 agctttctct tttgccaact ccatcatctc atcaggcaaa cctttagcgg ctaatttatc
     5161 cgggtgatgt tcgttcatta acttacgata agcccgtttc actgtatttt gatcgtcttg
     5221 tgcggttacg cctaatacgg tataggctgc ctcaatgctt ggttgtgcag gctgctgata
     5281 actgccctga tagccacctt gagattgctg atgataactc ccattttgct gataatagcc
     5341 accattgcgg aattgttgag ctgccataat cattgcgatc atctgctcaa attggaagcg
     5401 agacattccc attgtttcgg caatcgtaaa cagaatttgt tgctcgttca catcaagttg
     5461 cccatcttgc aaggctgcct gcatttgcac ttcgacaaag aaacgtaata agtcagttct
     5521 tgccccacag gcttctctga actcttgaat gacttggcgt aacgggaagt caggctcttt
     5581 acctaaggta aaggcatttt gcgctaattg ctgggctgca ctatctaatt ttaagcgagc
     5641 cattaacgct cgggctaatt caatatccgc ttcagtaaca cgcccttttg ctttcgcaat
     5701 atgccccaac accgcaaaag tggtttgggt gaaaagcgat tggcgagtta aatttttacc
     5761 gaaaagactt gaacgaactg aacctaattc atagagtttt ttatcggcta aatgcccgat
     5821 aaaagcgcca agtatcccac caaataaacc gtgaaaaagc tgatagccca agaaaaaacc
     5881 gactattttt ccgataaatt gcattttttt atccttttat agaaatgaat aaacgttatt
     5941 ctaacaaaat tttactttga ttgcttttta gttaatacaa acataagcca gcttgagaca
     6001 aaaataattg cataacctag ccaactcaac atatccgggt attgttcaaa gaacaacaac
     6061 cctaaaattg cactaaatag cacagaggca taactataaa ttgagatgtc tttcgccgcc
     6121 gcatatttat aggcggtagt tacacaaaac tgcccgaccg ctgaagttac ccctgctaac
     6181 actaagtaga ttgtctgtaa attggttagc actacataat caatggcgac aaaaggcaat
     6241 aatatcaagg tcgaaaaggc agaaaaaaag aaaacaatgg tattaggtga ttctactttt
     6301 ttggtttttg ccaataacct taagcataaa taggcaatgg ctgcaaacac agagccaatc
     6361 acgccaacca gataaggcat aaagcgaata tcaactgccg gtttgataat aaaaagtgcc
     6421 ccgataaagg caataaggca taaaatccat tgcaggagag atgctctctc ttttaaaaaa
     6481 atcgcagaaa gaataattaa aatgaacgac gtgagtttgc caatcatatc gacatcactt
     6541 agcacaaggt gatcgatgat atatacacca cataaaatcc ccaataaccc ggtgatggaa
     6601 cggcttaata ataacaaacg attttctgca taaccaaaga ataattggcg atttttccac
     6661 acaatataac cgcttatcaa tgctgtgcgt tgcgaaaaat cgctttttgt gcaatcggta
     6721 catcccccga aagacgaaga aaagcagcca taatcgcaaa gcttagtgcc gacaaaagca
     6781 cacaaaaaat ggctttaaat aacggcgaga gcgaattcca aaaattcata gcataaaggc
     6841 ttttaataaa aataaagccc tgattattca gggcttcaat ttagaatcaa ctaagaatta
     6901 gtattctaat actgcacgac ggtttttcgc gtagtcagct tctgagtgac ctaatactgc
     6961 tggtttctct tcaccgtaag aaacagttga agtgttgtta gcacctttag ctgctaagta
     7021 acctttaact gcatctgcgc gacgttgacc taatgcgatg ttgtactctg gagtaccacg
     7081 ctcatcagcg tgaccagcaa tagttacgtt accttgagtt tttgctaata attgagcgtg
     7141 agcgtctaat aattgacggt attcaccttc aactgcatag ctgtcaaaac cgaagtatac
     7201 agtgttgtat tgtgctttta attgttcagc tgttaaacca ccgaattcgc ctgctgcatt
     7261 tgctgctgca tttgctgctg cgtttgctgc tgcgtttgct gctgcatcat ctgatgagct
     7321 gctacatgct gctaatgcga aagctggtac tgctaccatt aatactttag caaatttttt
     7381 catttgttgc tcctaataga gaaatattat tatttagtta agtatggtga ccatgcaggg
     7441 aatttataat gtccacctgc gccaggcaaa ttagctttaa aacgcccatc tgcagacacc
     7501 aattgtagca ctttactcgt acctttggta gagctataaa taaccataat gccattcggt
     7561 gaaatacttg gactttcgtc taagaaagtt gaactcagta cttctgtgcc acctgaagcg
     7621 agatcgcgtt tcacaactct atcaccggca atcatgatta aattcttacc atcagatgat
     7681 actttcgcat tatagctacc gctaccgccc attgggctag caccaccacc tgatgaactc
     7741 attctataaa cttgaggaga acctgcacgg tcagacgtaa agtagattgt gctaccatct
     7801 ggagaccagc ttggttcagt attatttcca gcattagcgg tcaatttact tggtgtacca
     7861 ccgccactac ctacaacata aatatttaac tcaccatctt gatttgaggc aaacgcaatg
     7921 cgagaaccgt ctggagagaa tgatggcgca ccattatgtc ctttcaatgc tgccactaca
     7981 cggcgagcac ctgaacgaat atcgtgaaca acaacctgcg cttttttatt ttcaaatgtc
     8041 acataagcta atctgctacc atctggtgac cactctggcg acattaaagg ctctttactg
     8101 gttgtaatag taaaggcatt aaaaccatca taatcagata ctcgtaattc atacgatgat
     8161 acgccacgtt gaaccacata cgcaatttta gttctaaatg caccgcgaat ttgggttaat
     8221 ttttcaaata cctcatcact tactgtatgc gcaccttgac gaatttgggc tgccggaaca
     8281 ataaatgaac cttgggcaat gacaccaccc gctgtacccg atgtaccaag agtatcaatt
     8341 aattgataag cgacattata accgccacct gtgctactca cttggccaac tacaatatta
     8401 tcaataccga tagcactcca ttgatctgcc accacttccg ctgctgatcc cggttgagca
     8461 ggcatttgag agcgttcaag cggtgtaaat ttaccactat ttcgcaaatc atcagaaata
     8521 atttgtgcaa catctgccgg cacaccgctg gctttaaatg gcacaaccgc aatcggctga
     8581 gccatactta caccttcatc aatagaaatt cgcacatcag aatcagcaac cacctgagtt
     8641 gtcaataatg ctgaaaagat gcctattaca cccgatatac gagagaataa tttcatttgt
     8701 agtcctctaa attatttaac ttttaaacta aaatcaagtg ttggtgattt aaacatatta
     8761 tataccgcat ctgatggtgc aggtggtact tttctagttg ccactattgc acttaccgct
     8821 gcatcacata ctgctttatc gccactgata acttgatagt tagtaattgt gccatctcga
     8881 cttaagaata tcttaacatc acattgtttt ccggcaaaac ttggatcaac ccgatatcga
     8941 gattgaatta atttcttaat tcgttgacca tatgcattac catctacatt agtaccttgc
     9001 cctaagccac ttgtaccacc agaaccttgt gatcctgctg agtttttgtt accaccttta
     9061 cttgaaccac caccgatgtc tccactattg aggaaatcat caagtgcttt attgtttttc
     9121 gcttgggcgg cttttgcctc agcagctgcc ttcgctttag cttctttctc ggctttctct
     9181 ttggcttctt tttctgcttt tgctttggct tctttctcag ccttttcttt tgcttctttt
     9241 tctgccttgg ctttagcttc tttttctgct ttttccttcg cttctttagc ttcttttgcc
     9301 tgctgttcct tcagttcttt ttgctctttt aactttttct cagcttccgc tttcttctgc
     9361 tgttcaagct tcttttcaag tgcttcttta gcttttttct cttcttcagc ttgtttagca
     9421 gcagcctcta aacgcttagc ttctgcatct gctttcagtt ttgctgcttc agctgctttt
     9481 ttacgagttg cttcttctgc ttgctgcttt ttaagctctt cttgcttagc ttgttcttgc
     9541 ttttgctgtt cttgtagttt ttgctgacgc tcaatttctt gctgacgctg tttctcttga
     9601 atcgccgcta actcagcttg tttctgtttt tggatctctt ccggagtcgg tttatcttcc
     9661 acatcttctt caacagcttc ctctttaggc tctacctttg gcttcgatgc ttttgagcct
     9721 tttttctctg ccaataactg cccatattca gcagccactt gcccagtatc taccatcaca
     9781 gcttcaaagc tatcgctatc gccaccactg ccaccagccg cagcgagttc cgtcttagtg
     9841 aaaagagacc cgagcaataa caacccgatt aacaatatat gcaataagat cgaaacaata
     9901 accgctagct ctagtctatc ttgttgagat ttcacacgtt atccttggct atttaccttc
     9961 tgtcattaaa cctaccgatt tcactccggc gtctttaagt agtgtaatac ctttcataat
    10021 ttcttcataa ggtactactt tatcaccggc aactaaaaat aaggtattgt tatctttttg
    10081 aaattgttcc cctgcatagg caacaacttc tgattcatta atattctctt gcccattact
    10141 ttgcacatag ttaccgtcaa ttttcaattt atattcacca acacctgcca cctccaaaat
    10201 cactggagtt ttatcttcat tagaaactga ttggctatgc gtatcttccg gtaaattaac
    10261 ttctacactt tgactgataa ttggggccgt tgccataaaa atcagcaata ataccaacaa
    10321 tacatcaaga aatggaacaa tgttgatttc tgatttaatg tcattacgtt ttaaacgacg
    10381 agacatatat tcctctttac aggtgcttag caccttaatt gatctgaaat aaccggcaca
    10441 gtgtcccggt ctcgaataat gcggttacaa gcggtctttt ttacgaaaaa ctttacaaat
    10501 tttcattaaa aatcaaccgc ttgtagatta tctttttgaa aaagcctgac ggtgtaataa
    10561 ggtcgtgaac tcatcaatga aattcacata cccttgctca actttaccta aacgtaaatt
    10621 caaacggtta tatgccatta ctgcaggaat cgccgcaaat aaaccgatag ccgttgcaat
    10681 taatgcttct gcaatacccg gtgcgactga ttgtaaagtt gcttgcttaa ctgcacttaa
    10741 tcccataaag gagtgcataa ttccccaaac cgttccgaat aaaccaatat atgggctgat
    10801 tgaacccact gtgcctaaaa acggaatgta gttttctaaa ctttccattt cacggtttaa
    10861 tgctaagttc atcgcacggg tagaaccttg aataatcgat tctggcgcat ctgggttcgc
    10921 ttgttgtaaa cgggtaaatt ctttaaaccc aacataaaag atttgctctg aaccatttaa
    10981 tgcatcacga cgattctcta aaccttggtg taaacggttt agatcttccc ctgaccagaa
    11041 tcgatcttca aaagcaaggg aatctttttt ggctttagtt aaaattttgc tgcgctgaat
    11101 aatcaccgcc caagacaaaa ttgagaatac aataaggatc agcatcacga tctttaccac
    11161 aatacttgct tctaaaaata acgaaagaag actaaattca gtttgcatta ataaaactcc
    11221 gaatagtttt taaatagaaa aataactttt ttgtaatgct tgacgaatat catcaggaat
    11281 tgcaaccggc ttcattttga ttaaatcaac acaagcgacc gtaacatttg cctcactcag
    11341 acaatcgcca tctttccaca atcgttgttt aaagataagg gttgctttct tcaattctga
    11401 gagtgttgtt tccacattta ataaatcatc taaacgagca gggagcttat aatctatttc
    11461 aatcctctta acaacaaaag caagggattg gctaaaaagt tcctgctgtg agaaatgctg
    11521 ctgtctcaaa aattccgttc ttgcccgctc aaaaaaattc agatagttgg aatgataaac
    11581 tactccgccg gcatcagtat cttcataata aacacgaatc gggaaatcca taagcattat
    11641 ttctctcatt caaaatgaaa aaacgcccaa aatttaggcg caaattgtga gatattttag
//
LOCUS       DANLZC010000079        11683 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.079, whole genome shotgun sequence.
ACCESSION   DANLZC010000079 DANLZC010000000
VERSION     DANLZC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11683
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.079"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            57..713
                     /locus_tag="PXE44_001618"
     CDS             57..713
                     /locus_tag="PXE44_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5589118.1"
                     /translation="MTKKFTPFSDEDHAILASYFPMVDGIAALIGEHCEIVLHSLEFL
                     EHSAIYIANGHNTNRKIGSPITDRALKSLHHMATDSVSKPYFTKAKGGVLMKSITIAI
                     RNGKQHVIGLLCININLEVPTSQFLNSFIAPVDTASEVTFASSVEDLVSQTIQTTIDD
                     VMADRQISNNNKNRQIVISLFEKGIFDIKDSINQVAEKLDISRHTVYLYIRQIKQDND
                     "
     gene            713..1090
                     /gene="tusD"
                     /locus_tag="PXE44_001619"
     CDS             713..1090
                     /gene="tusD"
                     /locus_tag="PXE44_001619"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDL5589119.1"
                     /translation="MDYVLAIKSPVYGSQGAFLAYQVAEELLKANHTICQIFFFQEGV
                     SNANALVNPASDEVNLVEMWKKLAKLHRLPLHLCIAAAQRRGVVDEQISANATHNLAE
                     GFTLAGLGEFSQAVLKADRLLTF"
     gene            1092..1463
                     /gene="tusC"
                     /locus_tag="PXE44_001620"
     CDS             1092..1463
                     /gene="tusC"
                     /locus_tag="PXE44_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDL5589120.1"
                     /translation="MKKYKLAFLFTQPPFGSAVSREGLDALLAASAFCDEQEIAILFI
                     NDGVFNLIADQQPEKLLQKDHISTFKLLELYDLTECFICQESVSLRGLDNADWLLRML
                     QFKTHAEMIALLNQAEKVMTF"
     gene            1473..1748
                     /gene="tusB"
                     /locus_tag="PXE44_001621"
     CDS             1473..1748
                     /gene="tusB"
                     /locus_tag="PXE44_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDL5589121.1"
                     /translation="MLYTLSKANYDEETLNFFFSHLSENDAVVLWQDGVLQAVKSPQF
                     FAKLPRCYLLAQDVQARGLQEKLTAFQCISLSELVKLTAVYFPQVAL"
     gene            1864..2763
                     /locus_tag="PXE44_001622"
     CDS             1864..2763
                     /locus_tag="PXE44_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589122.1"
                     /translation="MVMAEENNTWVDKKHETVSTTLDRWANNINSWLGDADPDKPGTA
                     SLRVMLDSRWDKHDKFTYKPRVRGKIKLPILKKHLNVVFGDDELDNELTDKNHAGKLY
                     KNVNPNKRYNSREARDSNSSIGLRWSDGIKALGIETDLDAGIRSGSDIFGRLRISKDW
                     KITDQFSTRLEQIYRYGSESKHYLRTNFENKYTETDTTFIMNHTNFEYRHDVDEERSW
                     GNSLYRQHNFKPLTHISYGISMGGRLDKELSKFNYWGPFVSYRQPIWRNWLFIQPEIS
                     FYNDKDKDRKHFIQTFVRLEAIF"
     gene            3113..7138
                     /gene="rpoB"
                     /locus_tag="PXE44_001623"
     CDS             3113..7138
                     /gene="rpoB"
                     /locus_tag="PXE44_001623"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL5589123.1"
                     /translation="MAYSYSEKKRIRKSFGKRPQVLNVPYLLTIQLDSFEKFIEKDPE
                     GQQGLEAAFRSVFPIVSNNGATELQYVSYELGEPVFDVRECQIRGTTYAAPLRVKLRL
                     VSYDREAAAGTVKDIKEQNVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFARIDRRRKLPATIILRALGYTTE
                     EILNLFFEKIKFEIADNKLLMTLVPERLRGETASFDIAANGKVYVEMGRRITARHIRA
                     LEKDNVTQIEVPVEYIVGKVAAQDYVDLSSGEVICPANMEISMEMLANLSQAGYNQIE
                     VLFTNDLDHGPYISETLRVDPTYDRLSALVEIYRMMRPGEPPTKEAAEALFDNMFFSA
                     ERYDLSAVGRMKFNRSLEIPEGVGSGILSNDDIIGVMKKLIEIRNGRGEVDDIDHLGN
                     RRIRSVGEMAENQFRIGLVRVERAVRERLSLGDLDGVTPQDLINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHPTHYGRLCPIETPEG
                     PNIGLINSLSVYARTNNYGFLETPFRKVVNGQVTEEIEYLSAIEESNNIIAQANSNLD
                     ENFRFTDTYVTCRQQGESGLFKPEEVQYMDISTQQVVSVAAALIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMEKPIALDSGVAIVAKRGGTVQYVDASRIVVKVNEDE
                     TIPGEAGIDIYNLIKYTRSNQNTCINQVPCVNLGEPVARGEILADGPSTDLGELALGQ
                     NIRVAFMPWNGYNFEDSMLVSERVVQEDRFTTIHIQELSCVARDTKLGAEEITADIPN
                     VGESALSKLDESGIVYIGAEVKGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGTSGTVIDVQVFTRDGVEKDKRAKEIEEMQLCEAKKDLAEELEILEAGLF
                     TRVRNLLIEGGVKESALDGVAREKWLEQTLEDEAKQNQLEQLAEQHEELRKEFERKLE
                     IKRNKIIQGDDLAPGVLKVVKVYLAVKRQIQPGDKMAGRHGNKGVISKINPVEDMPYD
                     ENGQPVEIVLNPLGVPSRMNIGQILETHLGLAAHGIGDQIDKMIKQQQEVAKLREYMQ
                     KAYDLGHGSQVVDLSTFSDEEVMRLAQNLRKGLPLATPVFDGAHESEIKGLLELGGLP
                     TSGQITLYDGRTGEKFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKA
                     QFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGTHYMEPGIPE
                     SFNVITKEIRALAIDMELDEA"
     gene            7401..>11683
                     /gene="rpoC"
                     /locus_tag="PXE44_001624"
     CDS             7401..>11683
                     /gene="rpoC"
                     /locus_tag="PXE44_001624"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL5589124.1"
                     /translation="MKDLVKFLKAQTKSNEDFDVIKIGLASPDKIRSWSFGEVKKPET
                     INYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRER
                     MGHIELACPVAHIWFLKSLPSRIGLILDMPLRDIERVLYFESYVVTEPGMTDLEKNQL
                     LTEEQFMDAEERWGDEFEAKMGAEGIQALLRDLDLEHQCETLREELQETNSETKRKKI
                     TKRLKTLEAFLQSGNKPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLLDLVAPDIIVRNEKRMLQESVDALLDNGRRGRAITGSNKRPLKSLADMIKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFRPFIYSKLENRGIA
                     STIKAAKKMVEREDPIVWDILAEVIREHPILLNRAPTLHRLGIQAFEPLLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLTLEAQLEARALMMSTNNVLSPASGDPIIVPSQDVV
                     LGLYYMTREKVNGKGEGMYFLDPREAEKAYRTGQAELHSRVKVRVTEYEKNEAGELEA
                     KTSLVDTTIGRSILWMIAPKGMPFSLFNQTLGKKAISKLINESYRRLGLKESVVLADQ
                     IMYTGFAYAARSGASVGIDDMVIPEQKYAIIEAAEAEVAEIQEQFNGGLVTAGERYNK
                     VIDIWAAANERVAKAMMENLSTEEVINREGNPEKQASFNSIFMMADSGARGSAAQIRQ
                     LAGMRGLMARPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLT
                     RRLVDVAQDLVIIEDDCGTHEGIVMTPLIEGGDVKEALRDRVLGRVVAEDVLKPGTDE
                     VLIPRNTLLDEKWCDVVDRESVDVIKVRSVVTCDTDFGVCAKCYGRDLARGHLINQGE
                     AVGVIAAQSIGEPGTQLTMRTFHIGGAASAAAKESSIQVKNAGTIKLTNAKFVTNKEG
                     KIVLTSRNTELTVIDTFGRTKENYKVPYGAVLSKHDGAEVAVGEVVANWDPHTMPVIS
                     EVSGRIQFSDIVDGLTVTRQTDELTGLSSIVVQDVGERATAGKDLRPALRLVDSNGKD
                     ILIPGTDVAAQYFLPGKAIVTLDDGAEIEVGEALARIPQESVGTKDITGGLPRVADLF
                     EARKPKEPAILAEISGIVSFGKETKGKRRLVITPAEGEAFEEMIPKWRQLNVFEGEMV
                     QRGDVISDGAETPHDILRLRGVHAVTDYIVNEVQEVYRLQGVKINDKHIEVIVRQMLR
                     KAVITNAYDSEFLEGEQVEVARVKIANRKRAEEGKPLVEFERELLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKQDELRGLKENVIVGRLIPAGTGFAYHQARAKKRAQLEA
                     GIAVVPEFEAPAAKANSFVTDADIEAEFEFVADDATQSLAALLNAGEEE"
BASE COUNT         3480 a         2299 c         2705 g         3199 t
ORIGIN      
        1 atttattata atatttgcaa aaaatgtgat aaaactaacc gcttgtaatt tgtattatga
       61 ccaaaaaatt tacgcctttt tctgacgaag atcacgccat tcttgcttct tactttccga
      121 tggtagatgg gattgcggca ctgattggtg agcattgcga aattgtattg cattcgttag
      181 agtttctgga acattcggca atttatattg ctaacggaca caatactaac cgtaaaatcg
      241 gctctccgat tactgaccgt gcattgaaat cgctgcatca tatggcaacg gatagcgtct
      301 ccaaacctta ttttaccaag gcaaaaggtg gcgttttaat gaagtctatc actattgcga
      361 ttcgtaatgg taaacagcac gttatcgggt tactgtgcat taatattaat ttggaagtgc
      421 ctacctcaca gtttctcaat tcttttattg cccctgtaga taccgcttca gaagttactt
      481 ttgccagttc agtagaagat ttggtttcac aaacgattca aacgaccatt gatgatgtga
      541 tggcagatcg tcaaatttct aacaataata aaaatcgtca aattgtgatt tcgttatttg
      601 agaaagggat ttttgatatt aaagattcca ttaaccaagt agcggagaag ctggatattt
      661 cacgccatac tgtgtatctc tatattcgtc aaattaagca ggacaatgat taatggatta
      721 tgtattagcg ataaaatcac cggtttatgg ttcacaaggg gcatttttag cctaccaagt
      781 ggcggaagaa ttattaaaag ccaatcatac aatttgccaa atattctttt ttcaagaagg
      841 cgtgagcaat gctaatgcct tagtgaatcc agcaagcgat gaagtgaatt tagtcgagat
      901 gtggaaaaaa ttggctaaat tgcaccgctt gcctctgcat ttgtgcattg ctgctgccca
      961 gcgtagaggc gtggtggatg agcaaatctc agctaatgca acacataatt tagccgaagg
     1021 atttacccta gccgggttag gcgaatttag ccaagcagta ttaaaagcgg atcgattact
     1081 gactttttaa gatgaagaaa tataaacttg cctttttatt tacccagcca ccttttggtt
     1141 cggcagttag ccgtgagggg ctagatgcct tgcttgcagc tagtgctttt tgtgacgagc
     1201 aagagatagc tatcctcttt atcaatgatg gcgtatttaa tctcattgcc gatcaacagc
     1261 cggaaaaact cttacaaaaa gatcatattt ccaccttcaa attattggag ttgtatgact
     1321 taactgaatg ttttatttgc caagaaagtg taagtttacg agggctggat aatgcagatt
     1381 ggttactgcg aatgcttcaa tttaaaactc acgctgagat gatcgcattg ctcaatcaag
     1441 ctgaaaaagt aatgacgttt taaggaatat ttatgcttta taccctttcc aaagcgaatt
     1501 atgatgaaga aaccttaaac ttctttttct cacatcttag tgagaacgat gcggttgttc
     1561 tgtggcaaga tggcgtgcta caagcggtca aaagtccgca attttttgca aaattgcccc
     1621 gttgctattt attggcacaa gatgtgcaag ctcgaggatt gcaagagaag ctcactgcat
     1681 ttcagtgcat ttcattatcc gaattagtca aattaacagc ggtttatttt ccccaggttg
     1741 cactgtaacc tagagaggat attttttgta ataccaaata atttagatta tgatgtgatt
     1801 cctaaaaagg agaatattat gaaaacaaaa tgtcttattc cgctactagc acttacacct
     1861 gttatggtga tggcagaaga aaataacact tgggtagaca agaaacatga aacagtaagc
     1921 actaccttag atcgttgggc aaacaatatt aacagttggc taggtgatgc agacccagat
     1981 aaaccgggaa cggcttcttt acgggtaatg ttagatagcc gttgggataa acacgataaa
     2041 tttacttata agccccgtgt tcgtggtaaa atcaaactcc ctatcttgaa aaagcatcta
     2101 aatgtcgtat ttggtgatga cgaactggat aatgagctta ccgataaaaa tcatgctgga
     2161 aaattgtata aaaacgtcaa tccaaataaa cgctataact ctcgtgaagc gagagattcg
     2221 aactcttcta tcggtttacg ttggtctgat ggcattaagg ctttagggat tgagaccgat
     2281 ttagatgctg gtattcgttc gggaagcgat attttcgggc gtttacgcat cagtaaagat
     2341 tggaaaatca ccgaccagtt cagtacccgc ctagagcaaa tttatcgtta cggttcagag
     2401 agcaagcact atttacgtac taactttgaa aataaatata ccgaaacgga taccaccttt
     2461 attatgaacc acaccaattt tgaatatcgt catgatgtag atgaagaaag aagttggggg
     2521 aatagtttat atcgtcaaca taacttcaaa ccactcacgc acatcagcta tggtatcagt
     2581 atgggggggc gtttagataa agagttatca aaatttaact attggggtcc atttgtctcc
     2641 tatcgtcaac caatctggcg caactggctc tttatccagc ctgaaatcag tttttataac
     2701 gacaaagata aagatcgtaa gcactttatt cagacatttg tacgtttaga ggctatcttc
     2761 tagctattaa gcctcaagta gaatcttggg gcttgttttt gcaagcggtc aatttcttgc
     2821 caaaatttac aaaatccgct ataattccta accattttta gcaaaaattg gctgacggct
     2881 tgaccggtca gccttttgtg cttgaacgca agggctgatt ttgtcatttt aatcagcaaa
     2941 atttgcaaat ttcctgccca atttgaccgc ttgcagtagc gatttctgac tgcttgattg
     3001 gcgattggaa aatgacatct cgtacagttt ttataatgcc acttccaacc gagtctttcg
     3061 tgtcggattg tgtaatctca accaaaatat atttcagagg ctatcttaaa aaatggcata
     3121 ttcatattcc gagaaaaagc gtatccgtaa gagctttggc aaacgcccac aagttctgaa
     3181 cgtaccttat ctattaacaa ttcagcttga ttcttttgaa aaatttatcg aaaaagatcc
     3241 tgaagggcag caaggtttag aagcggcatt ccgttctgtt ttcccgattg tgagcaacaa
     3301 tggtgcaaca gaattacaat acgtttctta cgaattaggc gagccggtgt ttgacgtgcg
     3361 tgagtgccaa attcgtggta caacctacgc tgcaccatta cgcgtgaaat tgcgtttagt
     3421 ctcttacgat cgtgaagccg cagccggtac ggtaaaagac attaaagagc aaaacgtgta
     3481 tatgggcgaa atcccattaa tgaccgataa cggtactttc gtgattaacg gcaccgagcg
     3541 tgttatcgtt tctcaattac accgtagccc aggtgtgttc ttcgattctg acaaaggtaa
     3601 aacccactct tcgggtaaag tgctttataa cgcacgcatt atcccttacc gtggttcttg
     3661 gttagatttc gagtttgatc cgaaagataa cttatttgcc cgtatcgacc gccgtcgtaa
     3721 attgccggca accattattc tacgtgcatt aggctacacc accgaagaaa tcttaaattt
     3781 attcttcgag aaaattaaat ttgaaattgc cgataataaa ttattgatga cgttagtgcc
     3841 tgagcgttta agaggcgaga ctgcatcatt tgatattgca gcaaacggca aagtatatgt
     3901 tgaaatgggt cgccgtatta ccgctcgtca tatccgtgcg ttagaaaaag acaatgtgac
     3961 ccaaattgaa gtgccggtgg aatatattgt cggcaaagta gcggctcaag attacgttga
     4021 tttatcttca ggtgaagtga tttgcccggc gaatatggaa atctcgatgg agatgttggc
     4081 gaatttatca caagccggtt acaaccagat cgaagtatta tttaccaacg atttagacca
     4141 cggtccatat atttctgaaa ccttacgtgt tgatccaact tacgaccgtt taagtgcgtt
     4201 ggttgaaatc tatcgtatga tgcgtccggg cgaaccacca acgaaagaag cggcagaagc
     4261 attattcgat aatatgttct tctcggcaga gcgttatgat ttatcggcag taggtcgtat
     4321 gaaattcaat cgctcgttag aaattccgga aggcgtaggc agcggtattt taagcaacga
     4381 tgacatcatc ggtgtgatga aaaaattaat cgaaatccgt aacggtcgtg gtgaagtgga
     4441 tgatatcgac cacttaggta accgtcgtat ccgcagcgtt ggtgaaatgg cagaaaacca
     4501 attccgcatt ggtttggtgc gtgtagaacg tgcggtgcgt gagcgtttat ctttaggcga
     4561 tttagatggt gtaacaccac aagatttaat caacgctaaa ccgatttcag cagcagtgaa
     4621 agagttcttt ggttcatcac aactttcgca atttatggac caaaacaacc cgctttcaga
     4681 ggtaacacat aaacgccgta tctcggcatt aggcccgggt ggtttaaccc gtgagcgtgc
     4741 cggctttgaa gtgcgtgacg tacacccgac tcactatggt cgtttatgtc caatcgaaac
     4801 tcctgaaggc ccgaatatcg gtttgatcaa ctcactatcg gtgtatgctc gtactaataa
     4861 ctacggtttc ttagagactc cattccgtaa agtggtgaat ggtcaagtaa ccgaagagat
     4921 cgagtattta tctgcgattg aagaaagcaa caatatcatt gcacaggcga actcaaactt
     4981 agatgagaac ttccgtttta ccgatactta tgtaacttgc cgtcagcaag gcgaatcggg
     5041 cttattcaaa ccggaagaag ttcaatatat ggacatctca acccaacaag tggtttctgt
     5101 ggctgcggcg ttaattccgt tccttgagca cgacgatgcg aaccgtgcgt taatgggtgc
     5161 gaacatgcaa cgtcaggcag ttccaacctt gcgtgcggac aaaccattag tcggtaccgg
     5221 tatggaaaaa ccaattgctc ttgactcagg tgtagcgatt gtcgcaaaac gtggtggtac
     5281 ggtgcagtat gttgatgcgt cacgcattgt agtgaaagta aatgaagatg aaaccattcc
     5341 gggcgaagcg ggtatcgata tttataactt aatcaaatac acccgttcaa accaaaacac
     5401 ctgtatcaac caagtacctt gtgtgaactt gggtgagccg gttgctcgtg gtgaaatctt
     5461 agcggacggt ccttcaacag acttaggtga attggcatta ggtcaaaata tccgtgtggc
     5521 atttatgcct tggaacggtt ataacttcga agactcaatg ttagtttctg agcgtgtggt
     5581 acaagaagat cgctttacca ctatccacat tcaagaatta tcttgtgtgg cgcgtgatac
     5641 caaattaggt gcagaagaga tcaccgctga tattccaaac gtgggtgaat ctgcattaag
     5701 caaattagat gaatcaggta tcgtttacat cggtgcagaa gtgaaaggtg gcgacatctt
     5761 agtaggtaaa gtaactccta aaggtgaaac ccaattaaca ccggaagaaa aactattgcg
     5821 tgcgatcttc ggtgaaaaag cctctgatgt gaaagattca tcattgcgtg ttccaaacgg
     5881 tacttcaggt acggttatcg atgttcaagt atttacccgt gatggcgtag agaaagataa
     5941 acgtgcgaaa gaaatcgaag agatgcaact gtgtgaagcg aagaaagact tagcggaaga
     6001 gttggaaatc ttagaagccg gtttatttac tcgtgttcgc aacctattaa ttgaaggcgg
     6061 cgtaaaagaa tctgcattag atggcgttgc tcgtgaaaaa tggttagaac aaacccttga
     6121 agacgaagcg aagcaaaatc agttagaaca gttagcggaa cagcacgaag agttacgcaa
     6181 agaatttgaa cgtaaacttg agatcaaacg caacaaaatt atccaaggcg acgatttagc
     6241 accgggtgtg ttaaaagtag tgaaagttta ccttgcggtg aaacgccaaa tccagccggg
     6301 tgataagatg gcgggtcgtc acggtaacaa aggggttatc tcgaaaatca accctgtgga
     6361 agatatgcca tacgatgaaa acggtcagcc ggttgaaatc gtattgaacc cgctgggcgt
     6421 accgtctcgt atgaacatcg gtcagatctt agaaacccac ttaggcttag cggctcatgg
     6481 tatcggtgat cagattgata agatgatcaa acagcaacaa gaagtggcta aattacgtga
     6541 atatatgcaa aaagcctacg atttaggtca cggctcacaa gtcgttgatt taagcacttt
     6601 ctctgatgaa gaagtgatgc gtttagcgca aaacttacgc aaaggcttac cgcttgctac
     6661 tccggtattc gatggtgcac acgaaagcga aatcaaaggc ttattagagt taggcggctt
     6721 accgacttca ggtcagatca cactttatga tggtcgtacc ggtgagaaat ttgagcgtcc
     6781 ggtaaccgtt ggttatatgt atatgctcaa attgaaccac ttggttgatg ataaaatgca
     6841 cgctcgttca accggttctt atagccttgt tactcaacaa ccattgggtg gtaaagctca
     6901 attcggtggt cagcgtttcg gtgagatgga ggtttgggca ttagaagcat acggtgcggc
     6961 ttatactctt caagaaatgc ttacggtgaa atcggacgat gtgaacggtc gtacgaagat
     7021 gtataaaaac attgtagatg gtacacatta tatggagccg ggtatccctg aatcctttaa
     7081 cgtaattacc aaagagatcc gtgcattagc aattgatatg gagctggacg aagcgtaatg
     7141 gtttcactct cccccttgat gggggagaga caggcaaagg atttgttcag aatcctttgg
     7201 cagagagagg gtgatgcaag cggtgtaaat ttgctaaatt tttgcaaaaa tcgaccgctt
     7261 accctctccc tgattttgct tctgaacgag gcaaaatcta tccctctccc acaaggggag
     7321 agggtttaaa atcgcaacca aattcgtaga gtggatattc tttccaccaa acaaaacaac
     7381 ctcaaactag gggcaaaaaa gtgaaagact tagtgaagtt tttaaaagca caaacaaaat
     7441 cgaacgaaga ttttgatgtg attaaaattg gtttagcatc accggacaag atccgttctt
     7501 ggtcttttgg tgaagtgaaa aaaccggaaa ccattaacta tcgtaccttc aaaccggagc
     7561 gtgatggttt attctgtgca cgtattttcg ggccggtaaa agattacgag tgcttgtgtg
     7621 ggaaatacaa acgtttaaaa caccgtggcg taatttgtga aaaatgtggt gttgaagtaa
     7681 cccaaactaa agtacgtcgt gagcgtatgg ggcacattga attagcttgc cctgtcgcac
     7741 acatctggtt cttaaaatca ctaccgtccc gtatcggttt aatcttagat atgccgttgc
     7801 gtgatattga acgtgtgctt tattttgaat cttatgtagt tactgaaccg ggaatgaccg
     7861 atttagagaa aaatcagtta ttaaccgaag aacaatttat ggacgctgaa gagcgttggg
     7921 gtgatgaatt tgaagcgaaa atgggtgcag aaggtatcca agcattactt cgtgatttag
     7981 atttagaaca ccaatgcgaa accttgcgtg aagagttaca agaaaccaat tcagaaacca
     8041 agcgtaaaaa aatcactaag cgtttaaaaa ccttagaagc attcttacaa tccggcaaca
     8101 aaccggaatg gatggtgatg acagtattac cggtacttcc accggattta cgtccgttag
     8161 taccgcttga cggtggacgt tttgcgactt ctgacttaaa cgatttatac cgccgtgtaa
     8221 ttaaccgtaa caaccgttta aaacgcttgt tagatttagt tgcaccggac atcatcgtgc
     8281 gtaacgaaaa acgtatgtta caagagtcgg ttgatgcgtt attagacaac ggtcgccgtg
     8341 gtcgtgcgat taccggttcg aacaaacgtc cgttaaaatc tttggcagat atgatcaaag
     8401 gtaaacaagg tcgtttccgt caaaacttat taggtaaacg tgtagattac tcaggtcgtt
     8461 cggtaattac cgtaggccca tacttacgct tacaccaatg tggtttaccg aagaaaatgg
     8521 cgttggaatt attccgtccg tttatctact caaaattaga aaaccgtggc attgcttcaa
     8581 ctattaaagc cgcgaagaaa atggtagagc gtgaagatcc tatcgtatgg gatattcttg
     8641 ccgaagtgat tcgtgaacac ccgattctac ttaaccgtgc accaacactt caccgtttag
     8701 gtatccaagc gtttgagccg ttattgattg aaggtaaagc gatccagtta cacccactcg
     8761 tgtgtgcggc gttcaatgcg gacttcgacg gtgaccaaat ggcggttcac gttccattaa
     8821 cccttgaagc acagttagaa gctcgtgcgt taatgatgtc aaccaacaac gtactttcac
     8881 cggcaagtgg tgatccgatt atcgtaccgt ctcaggacgt tgtactcggt ctttactata
     8941 tgacccgtga gaaagtaaac ggtaaaggtg agggaatgta cttccttgac cctcgtgaag
     9001 cggaaaaagc ataccgtacc ggtcaggctg aattacattc tcgtgttaaa gtgcgtgtta
     9061 ctgaatatga gaaaaacgaa gcaggcgaat tagaagcaaa aacttcgtta gtagatacca
     9121 ccattggtcg ttcaatctta tggatgattg caccaaaagg tatgccgttt agcttgttca
     9181 accaaacctt aggcaaaaaa gcgatctcga aattaattaa cgagagctat cgccgtttag
     9241 gtttaaaaga atctgtggtt ttagctgacc aaattatgta caccggcttt gcttatgctg
     9301 ctcgttctgg tgcctcagta ggtatcgacg atatggtaat tccggagcaa aaatacgcaa
     9361 tcattgaagc ggctgaagcg gaagtcgctg agatccaaga gcaattcaac ggcggtttag
     9421 taacagccgg tgagcgttat aacaaagtga tcgatatttg ggctgctgca aacgaacgtg
     9481 ttgcgaaagc aatgatggaa aacctttcaa cggaagaagt aatcaaccgt gaaggtaatc
     9541 cggaaaaaca agcctcattt aacagtatct ttatgatggc ggactccggt gctcgtggtt
     9601 ctgctgcaca gattcgtcag ttagcgggta tgcgtggctt gatggctcgt ccggacggtt
     9661 cgattatcga aaccccaatt actgcaaact tccgtgaagg tttgaacgta ttacagtact
     9721 ttatttcaac ccacggtgca cgtaaaggtc ttgccgatac ggcattaaaa acagcgaact
     9781 ccggttactt aacccgtcgt ttagttgatg tggcacaaga tttagttatc attgaagatg
     9841 actgtggtac gcacgaaggt atcgtgatga caccacttat cgaaggtggt gatgtgaaag
     9901 aagccctgcg tgatcgtgta ttaggtcgtg tggtggcaga agatgtgtta aaaccgggta
     9961 cagatgaagt attaattcca cgcaacacct tattagatga aaaatggtgt gatgtggtgg
    10021 atcgtgaatc tgtggatgtg atcaaagtac gttcggtagt aacttgtgat acagacttcg
    10081 gtgtgtgtgc taaatgttac ggacgtgact tggctcgtgg tcacttaatc aaccaaggtg
    10141 aagcggtcgg tgttattgcg gcacaatcta tcggtgagcc gggtacacag ttaacgatgc
    10201 gtacgttcca tatcggtggt gcggcttctg cggcagcaaa agaatctagc atccaagtga
    10261 aaaacgcagg tacgattaaa ttaaccaacg ctaaatttgt aaccaacaaa gaaggcaaaa
    10321 tcgtattaac ttctcgtaat actgaattaa ctgtcatcga caccttcggt cgtaccaaag
    10381 aaaactataa agtgccttat ggtgcggtgc tttctaaaca cgacggtgca gaagtggctg
    10441 tgggtgaagt ggttgcgaac tgggatccgc acacaatgcc ggtaatctca gaggtatcag
    10501 gtcgtatcca atttagcgac atcgtagatg gcttaaccgt tactcgccaa accgatgaat
    10561 taaccggttt atcatctatc gtggtgcaag atgtgggtga acgtgcaacc gcaggtaaag
    10621 atttacgtcc ggcattacgc ttagttgata gcaacggcaa agacatctta attccgggta
    10681 cagacgttgc agcacaatac ttcttaccgg gtaaagcaat cgtaacctta gacgatggtg
    10741 cagaaattga agtcggtgaa gccttagccc gtattccaca agaatctgtg gggacgaaag
    10801 atattaccgg tggtcttcca cgcgtagcag acttattcga agcccgtaaa ccgaaagagc
    10861 cggcaatctt ggcggaaatt tcaggtattg tgtcgttcgg taaagaaacc aaaggtaaac
    10921 gccgcttggt aattactccg gcggaaggcg aagcgtttga agagatgatt ccaaaatggc
    10981 gtcagctcaa cgtatttgaa ggcgagatgg tacaacgtgg tgatgtgata tcagacggtg
    11041 cagaaactcc gcacgacatc ttacgcttac gtggcgttca cgccgttacc gactacatcg
    11101 taaacgaagt gcaagaagtt taccgcttac aaggggtaaa aattaacgac aagcacatcg
    11161 aagtgattgt tcgccaaatg ttgcgtaaag ctgtgatcac caacgcttac gactctgaat
    11221 tcttagaggg cgaacaagtg gaagtggctc gtgtgaaaat tgccaaccgc aaacgtgctg
    11281 aagaaggcaa accgttggta gaatttgaac gtgaattgct tggtattacc aaagcatctc
    11341 ttgcaacaga aagctttatt tctgcagcct cgttccaaga aaccactcga gtgcttaccg
    11401 aagccgcagt ggcaggtaaa caagatgaat tgcgtggctt aaaagagaac gtaatcgttg
    11461 gtcgcttaat cccggcaggt accggttttg cttaccacca agcacgagct aaaaaacgtg
    11521 cccaactgga agcaggaatt gcagttgtcc ctgaatttga agcaccggca gctaaagcca
    11581 attcattcgt taccgatgca gacatcgaag ctgaatttga atttgttgca gacgatgcaa
    11641 cccaaagttt agcagcgttg ttaaatgcgg gtgaggaaga gta
//
LOCUS       DANLZC010000080        11597 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.080, whole genome shotgun sequence.
ACCESSION   DANLZC010000080 DANLZC010000000
VERSION     DANLZC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11597
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.080"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(254..1849)
                     /locus_tag="PXE44_001625"
     CDS             complement(254..1849)
                     /locus_tag="PXE44_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5589125.1"
                     /translation="MRLSQLSLSVLLLSASSLVAAAPVNTLINCVATPPMKLSPAITN
                     DANDFNASSQQIYNRLLAFKAGELTVEPSLAERWEISDDGLSYTFHLRKDVKFHSNKQ
                     FTPTRTLNADDVVFSFQRQMDLNHPYHKVSDGTYFYYHWMNLPKILKTVEKVDDYTVK
                     ITLQQPNAPFLTTLAMDFLSIYSKEYADKLLAEGKPELLDQAPIGTGPFVFQAYQTDQ
                     AVRYVANPDYFQGKAKFEKLIFSITPDAGTRYAKLKAGECDVIDFPNISDIAQMKQDP
                     KVNLLQRDGLNLAYIGLNSTKPALNNAKVRQALHYATDKKAIVEAVYQGGGTVASNPF
                     PDSVLGYNPNLPQYEFNLEKAKALLTEAGFADGFETEIWVQPVVRPSNPNPRRTAEII
                     QADWAKIGVKAKLVTHEWADFNKRTREGEFSTGTYGWTSRNGDPDNFLFPLLSKSNIP
                     GTNYGRWTDEKFEQLLAQAVQTQNRDERAKLYQQAVEIANEQTPFIPLAHAINYVPLS
                     KRVQGFVQNPFGYTPFYGVSLSE"
     gene            1998..2438
                     /gene="mioC"
                     /locus_tag="PXE44_001626"
     CDS             1998..2438
                     /gene="mioC"
                     /locus_tag="PXE44_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDL5589126.1"
                     /translation="MTSICIITGSTLGGAEYVADHINEVLSEQGFEVELFNQATLADI
                     EGKTNLIIVTSTHGAGELPENIQQLFDDLANSSADFSSMKFGVIGLGSSDYDTFCNAV
                     NIVEEALKAKGASQVCESLRIDVMNNFDHDGSAEEWLPSFVEQL"
     gene            2450..2617
                     /locus_tag="PXE44_001627"
     CDS             2450..2617
                     /locus_tag="PXE44_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDL5589127.1"
                     /translation="MNKISANKAKWIKIAIILIYMFSPVDILPEAILGPFGLVDDAAA
                     LMLLIKTILEK"
     gene            complement(2706..3179)
                     /gene="asnC"
                     /locus_tag="PXE44_001628"
     CDS             complement(2706..3179)
                     /gene="asnC"
                     /locus_tag="PXE44_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDL5589128.1"
                     /translation="MYSKNTTKSEIDKLDQKILRWLIKDARTPYAEMAKVFGVSAATI
                     HVRVEKMRQAGIIESTKIVVDERKVGYDVCCFIGIILKSAKDYDKVIAKLSEFNEVVE
                     AYYTTGNYSIFIKVMTHTIEELHSVLAGKIQSIDEIQSTETLISLQNPISRDILP"
     gene            3380..4372
                     /locus_tag="PXE44_001629"
     CDS             3380..4372
                     /locus_tag="PXE44_001629"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDL5589129.1"
                     /translation="MKKSFILQQQEISFVKNTFTQYLIDKLGIIEVQGPILSEVGNGM
                     QDNLSGIEKAVKVNVKCIPSATYEVVHSLAKWKRHTLARFGFKEGEGLFVHMKALRPD
                     EDSLDRTHSVYVDQWDWEKVIPEGRRNFDFLKETVNEIYKAIRLTELAVEARFDIPSV
                     LPKQITFVHSEDLVQRYPNMTSKERENAICKECGAVFLIGIGGKLSDGKPHDGRAPDY
                     DDWTTESEQGYKGLNGDILVWNEELGTAFELSSMGIRVDETALRLQVALTGDEDRLEM
                     DWHKDLLAGRLPLSIGGGIGQSRLVMFLLRKKHIGEVQSSVWPKAVFEEFENIL"
     gene            complement(4496..4915)
                     /gene="atpC"
                     /locus_tag="PXE44_001630"
     CDS             complement(4496..4915)
                     /gene="atpC"
                     /locus_tag="PXE44_001630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL5589130.1"
                     /translation="MASQFELTVVSAERKIFEGTVTSVRVSGTDGELGVYAGHAPLLT
                     AIKPGMVKFTLADGKEEFIYVSGGFLEVQPTVVTVLADTAIRGEELDEQRILAAKRKV
                     EETLSKTSDADMTAKLAREIAKLRVYELTKTKLANKR"
     gene            complement(4943..6316)
                     /gene="atpD"
                     /locus_tag="PXE44_001631"
     CDS             complement(4943..6316)
                     /gene="atpD"
                     /locus_tag="PXE44_001631"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL5589131.1"
                     /translation="MATGKIVQIIGAVIDVEFPQDAVPKVYDALKVESGLTLEVQQQL
                     GGGVVRCIALGSSDGLKRGLKVENTNKAIEVPVGTKTLGRIMNVLGEPIDEAGPIGEE
                     ERWTIHRAAPSYEEQANSTELLETGIKVIDLVAPFAKGGKVGLFGGAGVGKTVNMMEL
                     IRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRVA
                     LTGLTMAEKFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGVL
                     QERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVVLSRNIASLGIYPAVDP
                     LDSTSRQLDPLVVGEEHYNVARGVQGTLQRYKELKDIIAILGMDELSEDDKRLVARAR
                     KIERFLSQPFHVAEVFNGVPGKFVPLKETIRGFKGILEGEYDHIPEQAFYMAGSIDEV
                     VERAKQM"
     gene            complement(6337..7203)
                     /gene="atpG"
                     /locus_tag="PXE44_001632"
     CDS             complement(6337..7203)
                     /gene="atpG"
                     /locus_tag="PXE44_001632"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL5589132.1"
                     /translation="MAGAKEIRTKIASVRNTQKITKAMEMVAASKMRKTQERMAASRP
                     YAESIRKAISHIAKGNIEYKHPFLTPRPVKKVGYLVVSTDRGLCGGLNINLFKTVLHE
                     LKEKDDQGVKSRLAVVGNKGISFFNPMGLEIKGHINGLGDTPAMEDLVGIVNGMVNAY
                     REGEIDEVYVVYNRFINTMSQKPTVQQLLPLPALENDSLEQTGSWDYLYEPNPQALLD
                     SLLVRYLESQVYQAVVDNLASEQAARMVAMKAATDNAGNLINELQLVYNKARQASITN
                     ELNEIVAGAAAI"
     gene            complement(7229..8770)
                     /gene="atpA"
                     /locus_tag="PXE44_001633"
     CDS             complement(7229..8770)
                     /gene="atpA"
                     /locus_tag="PXE44_001633"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL5589133.1"
                     /translation="MQLNSTEISELIKKRIAQFNVVSEAQSTGTIVSVSDGVIRIHGL
                     SDVMQGEMIALPGNRYAIALNLERDSVGAVVMGPYADLAEGMQVQCTGRILEVPVGRG
                     LLGRVVNTLGQPIDGKGEIQNDGFSPIEVIAPGVIDRQSVDQPVQTGYKAVDSMVPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTIANVVRKLEEHGAL
                     ANTIVVVASASESAALQYLAPYAGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERASRVNADYVERFTNGAVKGQTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLESGLFNAGIRPAVNPGISVSRVGGSAQTKLVKKLAGG
                     IRTALAQYRELAAFAQFASDLDEATRKQLSHGQKVTELLKQKQFEPMSVAQLGLSLAA
                     AEYGYLDDIPVERVGSFEANLLAYAQSNYGEFMQELSKSGNFNDEIKDKLNEILSGFK
                     KNSAW"
     gene            complement(8783..9316)
                     /gene="atpH"
                     /locus_tag="PXE44_001634"
     CDS             complement(8783..9316)
                     /gene="atpH"
                     /locus_tag="PXE44_001634"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL5589134.1"
                     /translation="MSELSTVARPYAKAAFDFALEQGQLEQWQQMLTFSAAVANDEQV
                     ASFLNSSLASGQISETFIKICGEQLDQYGQNFIRVMADNKRLAVLPAVLNAFLELRAE
                     QESIKDVEVVSADKLTDAQEAKIANAMEKRLGTKVRITSSIDSSLIAGVIIRYDDVVI
                     DGSSRGQLNRLSQELSL"
     gene            complement(9330..9800)
                     /gene="atpF"
                     /locus_tag="PXE44_001635"
     CDS             complement(9330..9800)
                     /gene="atpF"
                     /locus_tag="PXE44_001635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL5589135.1"
                     /translation="MNLNATLIGQLIAFALFVAFCMKFVWPPLIRVIEERQANIASAL
                     ASAEKAKQEQADSKAAADQEILRAKEEAQNIIDLATKRRNEILESVQAEAEVERQRII
                     EQGYAEVESERKRVQEELRQKVAALAIAGAEKIVGRSVDAAANNDIIDKLVAEL"
     gene            complement(9861..10115)
                     /gene="atpE"
                     /locus_tag="PXE44_001636"
     CDS             complement(9861..10115)
                     /gene="atpE"
                     /locus_tag="PXE44_001636"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDL5589136.1"
                     /translation="MESVITATIIGASILLAFAALGTAIGFAILGGKFLESSARQPEL
                     ASSLQTKMFIVAGLLDAIAMIAVGISLLFIFANPFIDLLK"
     gene            complement(10233..11021)
                     /gene="atpB"
                     /locus_tag="PXE44_001637"
     CDS             complement(10233..11021)
                     /gene="atpB"
                     /locus_tag="PXE44_001637"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL5589137.1"
                     /translation="MAGTTAEYISHHLSFLSTGEGFWAVHLDTLFFSLVAGAIFLWVF
                     SRVAKNATTGVPGKLQCFVEIVIEWVNGLVKDNFHGPRNVIAPLALTIFCWVFIMNAI
                     DLIPVDFLPQLANLFGIHYLRAVPTADISATLGMSICVFALILFYTVKSKGFGGLVKE
                     YTLHPFNHWAFIPVNFILETVTLLAKPISLAFRLFGNMYAGELIFILIAVMYMADNFA
                     LQALGIPLHLVWAIFHILVITLQAFIFMMLTIVYLSIAYNKADH"
     gene            complement(11044..11427)
                     /locus_tag="PXE44_001638"
     CDS             complement(11044..11427)
                     /locus_tag="PXE44_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDL5589138.1"
                     /translation="MSAVINRAKSQYKQALKVEFFLLCVFALGVSLWKMNFNFSFLGG
                     ALASFLPHLVFVYWVFFKKSAKNQSKMTAFYRGEGMKWLVTIILVIGCFKLIPALDVV
                     LFFVGYFIALFLNNMIPFVLSKHHH"
BASE COUNT         3469 a         2529 c         2173 g         3426 t
ORIGIN      
        1 gtgtagctca atttaaacaa gctccgctac cttttacagg tcaaaagcgg atgtttttaa
       61 aacactttta atgatgattt aaagggcgtt tgttttcagt ttattttgca taacttttgt
      121 gattacggct atgcaaaatg atttgcaaaa ctatgcaaaa taaatcgacc gcttacaatt
      181 ctaaaagaaa agcagtctat tctctaccac attttgcaaa aaagtggcaa aaatagaccg
      241 cttgtatggc atatcactcc gataaactca ccccataaaa tggcgtgtag ccaaacggat
      301 tttgcacaaa accttgcacc cgtttgctga gtggtacata attgatagcg tgagccagcg
      361 gaataaatgg cgtttgttcg ttggcaattt ccaccgcttg ttgatagagc ttcgcccgct
      421 cgtcacggtt ttgggtttgt acagcttgag ctaataactg ctcaaatttc tcatccgtcc
      481 aacgcccata atttgttccc ggaatattag atttgcttaa taaagggaag aggaaattat
      541 caggatcacc attacggctt gtccaaccat aagtgccggt agagaactcg ccttcacggg
      601 tgcgtttgtt aaaatccgcc cattcgtggg tcactaattt cgctttcacc ccgattttcg
      661 cccaatccgc ctgaataatt tcagccgttc gacgtgggtt tgggttagaa gggcgcacta
      721 ccggttgtac ccaaatttcc gtttcaaatc catcggcaaa gccggcttct gttaataggg
      781 ctttggcttt ttcaaggtta aattcatatt gcggtaaatt tggattatag cccaacaccg
      841 aatcagggaa cggattactt gccaccgtgc caccgccttg ataaacagcc tcgacaatcg
      901 ctttcttatc ggttgcataa tgcaaggctt ggcgaacttt ggcgttattt aatgccggtt
      961 tggtgctgtt taagccgata taagctaaat tcagcccgtc tctctgtaac aaattcacct
     1021 tcggatcttg tttcatttgg gcaatatcgg aaatattcgg gaaatcaatc acatcgcatt
     1081 cacccgcttt cagtttggca taacgagtgc ccgcatcagg ggtaatacta aaaatcagtt
     1141 tctcaaattt tgctttacct tggaaataat ccgggttcgc aacataacgc accgcttgat
     1201 cggtttgata ggcttggaac acaaaaggcc ctgtgccgat cggggcttga tccaataact
     1261 ccggcttgcc ttccgccagc aatttatccg catattcttt tgaataaatc gacaagaaat
     1321 ccatcgctaa ggttgtcaga aaaggagcat taggttgctg taaggtgatt ttaacagtgt
     1381 aatcgtccac tttttccact gtttttaaaa tcttcggcaa attcatccaa tggtaataga
     1441 aataagtacc gtcagagacc ttatggtaag ggtggtttaa atccatctgg cgttggaacg
     1501 agaacactac atcatccgca ttcagcgttc gagttggcgt aaattgttta ttggaatgaa
     1561 atttcacgtc tttacgtaaa tggaatgtgt aacttaagcc atcgtcactg atttcccaac
     1621 gttccgccaa actcggctca accgttagtt caccggcttt aaacgctaat aagcgattat
     1681 aaatttgttg tgaactggcg ttgaaatcat tggcatcatt agtaattgca ggactaagtt
     1741 tcatcggtgg agttgccacg caattaatca acgtatttac cggagctgct gccaccaaac
     1801 tgctggcact taagagtaaa acagaaagag ataattgaga aagacgcata aagactcctt
     1861 aacattttct actaacttac tcggcattaa cgtattttca aaataactaa aggttataat
     1921 ttataatcag aataatcagt tgcaaaccaa tgattcttgg ctagaatatt ggcagttttt
     1981 taaacgagag atttactatg acatcaattt gtatcatcac cggcagcacc ttaggcggtg
     2041 cggaatatgt tgccgatcac attaatgagg tattaagcga acaaggtttt gaagtggaac
     2101 tgtttaacca agccaccctt gccgatattg agggcaaaac caatctgatt atcgttacct
     2161 ctacccacgg agccggcgag ttacccgaaa atatccaaca gctatttgac gatttagcaa
     2221 atagctcggc tgatttcagc agtatgaaat ttggtgtaat cggtttaggt agctccgact
     2281 atgacacctt ctgcaatgca gtaaatattg ttgaagaagc tctcaaagcg aaaggggcaa
     2341 gccaagtttg cgaatcactg agaattgatg taatgaataa tttcgaccac gatggcagtg
     2401 ctgaagaatg gttaccatca tttgttgaac aattataact taagtatcaa tgaacaaaat
     2461 cagtgcaaat aaggcaaagt ggattaaaat cgctattatc ttaatctata tgttcagccc
     2521 tgtagatatt ctacccgaag cgattttagg cccattcggt ttggttgatg atgccgccgc
     2581 attaatgcta ttgattaaga ctattttgga aaaataggca tagtagacaa aatagttgca
     2641 aaaaaccaaa acaaaacgac cgcttgtaac ccaatacaag tggtcttatc ttttacttaa
     2701 ttcttttaag ggagaatatc tcttgaaata ggattttgaa gtgaaataag cgtttctgtt
     2761 gactgaattt catcaataga ctgaattttt cctgctaata ccgaatgcaa ctcttcgatg
     2821 gtatgggtca ttactttaat aaaaatagaa taattaccag tggtatagta cgcctcaaca
     2881 acttcattaa attcactaag tttagcaatt actttatcat aatctttggc tgattttaaa
     2941 ataatgccaa taaagcaaca cacgtcatac ccaaccttac gctcgtccac tacgattttg
     3001 gtagattcaa taatgccggc ttggcgcatt ttttcaacac ggacatggat agttgcggca
     3061 ctcacaccaa aaacttttgc catttccgca taaggcgtgc gagcatcttt aattagccaa
     3121 cgtaaaattt tttgatccag cttatcaatt tccgatttag tcgtgttttt actgtacatt
     3181 ttttctcctc aaaatcaaac taattagata agatttaaaa atattgcaaa tattttaaat
     3241 gaaatttatc acaaatgctt ttttatttga taaatattgc attttttaga tatttcttta
     3301 aattagcact catcaattct taaatgcaat caagcacgag aaataaaaaa tcaacattct
     3361 ttaattagga aagcaaacaa tgaaaaaatc ttttatttta cagcaacaag aaatcagttt
     3421 tgttaagaac acatttactc aatatttaat tgataaatta ggcattattg aagttcaggg
     3481 gcctattttg agcgaagtcg gcaacggtat gcaagacaac ctttccggta tcgaaaaagc
     3541 agtaaaagta aacgtaaaat gtattccaag cgctacttat gaagtggttc attctcttgc
     3601 aaaatggaaa cgccacacct tagctcgttt tggatttaaa gaaggagaag ggttatttgt
     3661 tcatatgaaa gctcttcgcc ctgatgaaga ttctttagat cgcacacact cggtatatgt
     3721 tgaccaatgg gactgggaaa aagtcattcc tgaaggtcgc cgcaacttcg atttcttaaa
     3781 agaaacggta aacgaaatct ataaagcaat tcgtttaacc gaattagcgg tggaggctcg
     3841 tttcgatatt ccatctgtat tgcctaaaca aattactttc gttcacagcg aagacttagt
     3901 gcaacgctat ccgaatatga ccagcaaaga gcgtgaaaat gctatctgta aagagtgcgg
     3961 agcggtgttc ttaatcggta tcggcggcaa attatcagac ggtaaacctc acgatggacg
     4021 agctccggac tatgatgact ggacaaccga gtccgaacaa ggctacaaag gcttaaacgg
     4081 cgatatttta gtttggaacg aagaactcgg cacagccttt gaactttcct caatgggtat
     4141 tcgtgtagat gaaaccgcct tacgcttaca agttgcttta actggcgacg aagaccgcct
     4201 cgaaatggat tggcacaaag atttattagc cggcagactc ccgctttcta tcggcggcgg
     4261 tatcggtcaa tctcgcttag tgatgttctt actacgcaaa aaacacatcg gtgaggtgca
     4321 atccagtgtc tggccaaaag cggtatttga agagtttgag aatattcttt aataaaacaa
     4381 aaaagcgaac cttagggttc gcttttgctt tctacaagcg gtcttatttt acaaaaaatt
     4441 tactgttttt aaccgcttgt aaaacaaaac cccactttcg tggggttttt tgatattaac
     4501 gtttattcgc taatttggtt ttagtgagtt cgtaaacacg cagtttcgca atttctctcg
     4561 ctaacttcgc ggtcatatcc gcatcgctgg tttttgaaag tgtttcttct actttgcgtt
     4621 ttgcggcaag aatacgctgt tcgtctagtt cttcaccacg aattgcggta tcagccaaca
     4681 ctgttactac ggtcggttgt acttcaagga aaccacctga aacgtagata aactcttctt
     4741 tgccgtcagc taaagtgaat ttaaccattc ccggtttaat tgcggttaat aacggtgcat
     4801 gccctgcata cacccctaat tcaccatctg tccctgaaac acgcacgctg gtaactgtgc
     4861 cttcaaagat tttacgttct gcactcacta ccgtgagttc aaattgagat gccatgttgt
     4921 tctcctttag tttagaacaa aattacatct gttttgctct ttcaaccact tcgtcaattg
     4981 aacccgccat atagaacgct tgttccggaa tatggtcgta ttcgccttct aaaatgcctt
     5041 taaagccacg aattgtttct tttaacggta cgaatttgcc cggtacgccg ttgaatactt
     5101 ccgcaacgtg gaatggttgt gaaaggaaac gttcgatttt acgcgcacga gcaacaaggc
     5161 gtttgtcgtc ttccgataat tcatccatac ccaaaattgc aatgatgtct tttaactctt
     5221 tataacgttg taatgtacct tgtacaccac gagcaacgtt atagtgttcc tcaccaacca
     5281 cgagtggatc taactgacga gaggttgagt caagagggtc aaccgccggg taaataccga
     5341 gtgaagcgat attacggctt aataccaccg ttgagtctaa gtgggcaaag gtagttgccg
     5401 gagacgggtc agttaagtca tccgccggaa catataccgc ttgcacagag gtaatagagc
     5461 cggttttggt tgaggtaata cgctcctgta atacgcccat ctcttctgca agagtcggct
     5521 gataacctac cgcagacggc atacgaccta ataacgcaga tacttcggta ccagctaaag
     5581 tataacggta gatgttatcc acgaagaata atacgtcacg accttcatca cggaattttt
     5641 ctgccatggt taagccggtt aatgccacac gcagacggtt gcccggtggt tcgttcattt
     5701 gaccgtaaac cagcgatact ttgtctaata cgttagagtc tttcatttcg tggtagaagt
     5761 cattaccctc acgagtacgc tcacctacac cggcgaatac cgagtaaccg ctatgttcga
     5821 tagcgatgtt acggattaac tccatcatat ttacggtttt acctacaccc gcaccaccga
     5881 ataagccaac tttaccaccc ttagcaaatg gagcaactaa gtcgataact ttgataccgg
     5941 tctctaaaag ctcggtgctg ttcgcttgtt cttcgtagct tggtgctgca cggtggattg
     6001 tccaacgttc ttcttcaccg attgggcctg cttcatcaat tggctcacct aatacgttca
     6061 taatacgacc gagtgttttt gtaccaaccg gtacttcaat cgctttattc gtattttcaa
     6121 cttttaagcc acgttttaaa ccatcagatg aaccaagtgc gatacaacgg accacaccgc
     6181 cacctaattg ttgttgaact tcaagggtta atcctgattc aacttttaat gcatcataaa
     6241 cttttggtac tgcatcttgc ggaaactcaa catcgattac cgcaccgatg atctgtacaa
     6301 tttttcccgt tgccattacc gttcctctat tcattgttaa attgctgcgg cacccgcgac
     6361 aatttcattt aattcattcg taatacttgc ttgacgagct ttgttataca ctaactgtaa
     6421 ctcattaatc agattacctg cgttatcggt tgctgctttc attgccacca ttcgagcagc
     6481 ctgttcagac gcaagattat ctaccactgc ctgataaact tgagattcta aataacgaac
     6541 cagtaagctg tctaataacg cttgtggatt tggttcatag agataatccc aagaaccagt
     6601 ttgctctaat gagtcatttt ccagtgcagg caaaggaagc aactgttgta ctgtcggttt
     6661 ttgtgacatc gtgtttataa aacggttata taccacatac acttcatcaa tttcgccttc
     6721 acggtaggca tttaccatac cattaacaat accgactaaa tcttccattg ccggtgtatc
     6781 acccaatcca ttgatatgac ctttaatctc tagccccatt gggttaaaaa aggagatccc
     6841 tttatttccc accacagcaa gtcgagactt aacaccttgg tcatcttttt ctttcaattc
     6901 atgtaaaacg gttttaaata aattgatatt taagccacca cataaaccgc gatcggttga
     6961 aactactaaa tagccaactt tttttaccgg acgtggggtc aaaaatgggt gtttatactc
     7021 aatgttacct ttggcaatat ggctaattgc cttgcgaata ctttcagcat aagggcgaga
     7081 agccgccata cgctcttggg ttttacgcat ttttgatgcg gcaaccattt ccatcgcttt
     7141 ggtaattttt tgtgtattac gaacacttgc aattttggtt cttatctctt tagcacctgc
     7201 catattattt ctccgctaaa aagttagatt accaagcact gttctttttg aaaccagata
     7261 aaatctcgtt caatttatct ttaatttcat cgttgaaatt gccggatttt gacagttctt
     7321 gcataaactc gccgtagtta ctttgagcat aagctaataa gtttgcttca aatgagccaa
     7381 cacgctcaac cggaatatca tctaagtaac cgtattcagc tgctgctaat gataaaccta
     7441 attgtgcaac agacatcggc tcgaattgtt tttgtttcaa caattcagtt actttttgac
     7501 cgtgtgaaag ctgtttacgt gttgcttcat caagatctga tgcgaactgg gcaaatgccg
     7561 ctaattcacg gtattgagca agtgcggtac ggataccacc ggcaagcttc ttaaccagtt
     7621 tagtttgtgc agaaccacct acacgggaaa ccgagatacc cgggtttacc gcagggcgaa
     7681 taccggcatt gaataagcct gattctaaga aaatctgacc atcggtaatt gaaattacgt
     7741 tagtcggtac gaaggctgaa acgtcacctg cttgggtttc gatgatcggt aatgcggtta
     7801 aagaaccggt ttgacctttc acagcaccgt tagtgaaacg ttctacatag tctgcattta
     7861 cacgagacgc acgctctaat aagcgagagt gtagatagaa cacatcaccc gggaatgctt
     7921 cacgacccgg tggacggcgt aataacaatg aaatttgacg gtaagcaact gcttgcttag
     7981 acaaatcatc gtaaacgatc aacgcatctt caccacggtc acggaagtat tcacccattg
     8041 cacaacctgc ataaggtgca agatattgta atgcagcaga ttcagacgca gaagccacta
     8101 caacgatagt gttcgctaat gcaccgtgtt cttctaattt acgcaccacg ttggcgatag
     8161 ttgatgcctt ttgaccgatt gcaacgtaga tacatttaat gcctgaatca cgttggttga
     8221 tgatcgcatc aatcgctaat gcggttttac cggtttgacg gtcacctata atcaattcac
     8281 gctgaccacg accgattggt accattgagt ccaccgcttt gtaaccggtt tgaaccggtt
     8341 gatcaaccga ttgacggtcg ataacgcccg gtgcgatcac ttcgattggt gagaaaccgt
     8401 cattttggat ttcgccttta ccatcgattg gctgacctaa ggtatttaca acacgaccta
     8461 ataagccacg accaaccggc acttctaaaa tacgaccggt acattgtact tgcatacctt
     8521 ctgctaaatc ggcataaggt cccattacaa ctgcaccaac agaatctctt tctaagttta
     8581 atgcaatcgc ataacgattg cccggtaatg caatcatttc accttgcatt acatcagata
     8641 agccgtggat acgaataacc ccgtcactta ccgaaacgat tgtccctgtg ctttgagctt
     8701 cactcaccac attaaactgg gcaatacgtt ttttaatcaa ttcactaatt tcagttgaat
     8761 ttagttgcat tttttattcc tcttacaagc tcaactcttg actcaagcgg tttagctgtc
     8821 cacgactact accatcaatc actacatcat cataacgaat gatcacaccc gcaattaatg
     8881 aactatctat tgatgaagta atacgtactt tcgttccgag tcgcttttcc atcgcattgg
     8941 caatttttgc ttcttgtgca tctgttaatt tatctgcaga aacaacctca acatctttaa
     9001 ttgattcctg ttctgctctt aattctaaaa atgcattcaa taccgcaggt aatactgcta
     9061 aacgcttgtt atcagccatt acgcgaatga aattttgccc atattgatct aattgttcgc
     9121 cacaaatctt gataaacgtc tcagaaatct gaccgcttgc aagtgatgag ttaagaaaac
     9181 tagctacttg ctcatcgtta gcgacggcgg cagaaaaagt taacatctgc tgccattgtt
     9241 ccaattgacc ttgttctaaa gcaaaatcga aagccgcttt agcatagggg cgagctactg
     9301 tgcttaattc tgacatctgc ccctcctagt tatagttccg caactagctt atcaataata
     9361 tcgttgttcg cagcggcatc caccgaacga cccacgattt tttctgcacc agcaatagcc
     9421 aatgcagcca ctttttgacg aagctcttct tgaacgcgtt tacgttcgct ttcaacttcc
     9481 gcgtaaccct gttcaataat acgttgacgt tccacttcag cttctgcttg tacagattct
     9541 aaaatttcat tacgacgttt tgttgctaaa tcaatgatat tttgtgcttc ttctttcgct
     9601 ctaaggattt cttgatccgc agccgcttta gaatcagctt gttcttgttt cgctttttcg
     9661 gcagaagcca atgcgctagc aatatttgcc tgacgctctt ctatcactcg aattagcggt
     9721 ggccatacaa acttcataca gaacgcaaca aaaagtgcga acgcaatcag ttgaccaatt
     9781 agtgttgcat ttaaattcac aacgccctcc taaaagccgg taaagttatt caactgaata
     9841 acggttaatc aaatcccgac ttatttcaat aaatcaatga acgggtttgc gaagatgaat
     9901 agtaatgaaa tacctacagc gatcattgcg atagcatcta aaagaccagc aacgataaac
     9961 attttagttt gtaagcttga tgctaattcc ggctgacgtg cagatgactc taagaatttg
    10021 ccacctaaga tagcaaagcc gatagcagta ccaagagcag cgaaagctaa taaaattgaa
    10081 gcaccaataa ttgttgctgt gattactgat tccataatgt tctccattag attgaggaat
    10141 tagcccgaag gctgggttgt tgataaaaat acatatcaag cggtcaatat tttgcaaaaa
    10201 tttgcgaaaa acctaccgct tgtaaggcta gtttaatgat ccgctttgtt ataagcgata
    10261 ctcaaataaa caatcgttag catcataaaa ataaaggctt gaagcgtaat aactaaaata
    10321 tggaaaatcg cccaaaccaa gtgtaatgga atccccaacg cctgtagagc gaagttgtct
    10381 gccatataca ttactgcaat aaggataaag attaactcac ctgcatacat attaccgaat
    10441 aaacggaacg ccaatgaaat cggttttgcc aaaagcgtta ccgtttcaag aataaagttt
    10501 accggaatga atgcccagtg gttaaacggg tgtaacgtgt attctttcac taaaccgcca
    10561 aaacctttcg atttcaccgt gtaaaataaa attaacgcaa atacacaaat agacataccc
    10621 agtgttgcac taatatctgc cgttggtact gcacgcaaat agtgaatacc aaacagattg
    10681 gctaactgtg gtaagaaatc gaccgggata aggtcgatcg cattcataat aaacacccaa
    10741 cagaaaatcg ttaatgctaa tggtgcaatt acattgcgag gtccgtggaa gttatccttc
    10801 actaaaccgt ttacccactc aataacaatc tcaacaaaac attgcaattt tcccggaacg
    10861 cccgttgttg cattttttgc tacacgcgag aacacccata aaaaaatcgc acctgccact
    10921 aaagagaaga acagtgtatc taaatgcact gcccaaaagc cttcacctgt cgataaaaaa
    10981 ctcaagtgat ggctaatata ttctgctgtc gttccagcca taacatatcc ttttaaatta
    11041 aaattagtgg tgatgtttgc ttaatacaaa cgggatcata ttatttaaaa ataatgcgat
    11101 aaaataaccc acgaaaaaca acacaacatc tagagcaggg ataagtttaa aacagccgat
    11161 caccaaaata atagtaacca accatttcat tccttcacca cgatagaaag cggtcatttt
    11221 tgactgattt tttgcagatt tcttaaagaa aacccaatat acaaacacaa gatgaggcaa
    11281 aaaactcgct aaagcaccgc ctagaaagga aaaattaaaa ttcattttcc ataaagacac
    11341 tccaagtgca aaaacgcaca agagaaaaaa ttcaaccttt aatgcttgtt tatattgtga
    11401 ttttgctcta ttgattacag ctgacacatt ttatcctttt tgaataagta aaccacacaa
    11461 tcaggctaat aattagccac tgttgaatca aagaaaatcc gtgctaaatt atactttaca
    11521 aatgaattgt tgcaaccgaa aagcgacaca aaaacttgtt attcttcaca tttttgaaaa
    11581 aaaattttat ttacgtt
//
LOCUS       DANLZC010000081        11432 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.081, whole genome shotgun sequence.
ACCESSION   DANLZC010000081 DANLZC010000000
VERSION     DANLZC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.081"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..765
                     /locus_tag="PXE44_001639"
     CDS             <1..765
                     /locus_tag="PXE44_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5589139.1"
                     /translation="AGTLLKELFETKLPVFSAPKNPNFLRHLITLFEDKNFISLDFFA
                     GSSSFPHAILESNRIDKGNRKFIAVQYPEEIDIKSKNGKVAKQFCQRKNLPLYITEIS
                     KERIRRAGKQILQNNPENRPLDVGFKVFKLTESHFKQWQSPTSENLADQLEMFIDPVS
                     EKADTQAVLYELLLRLGLKLTAKVRLENKVFWVEENGLIFALLLNAADEEIIQTVIAQ
                     QPKKVIALDRLFNGNDARKKNTELQMQDAGITFFVI"
     gene            775..3411
                     /locus_tag="PXE44_001640"
     CDS             775..3411
                     /locus_tag="PXE44_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5589140.1"
                     /translation="MELQFETLEYQLQAVNATVNLFVGQPNAATEFSLKAQNDMRFVP
                     NLGLQISDEQLQQNLANLQNRQKIDRTLLVEQGKNFTVEMETGTGKTYVYLRTIFELN
                     RQYGWQKFVIVVPSVAIREGVLHTLETTKSHFNTVFDNPSVNQKFEYKSNQTSRLKSF
                     ASANHIEILVMNIDAFTKESNVINTVNESGDAPIFYIQQANPIVIIDEPQNMETEIRR
                     NAIESLSPLFTLRYSATHKNAYNPIFSLNPVQAYEMGLVKQIEVDSVLAENEVNGAYL
                     SLKEIKTGAKSWSAKIEILFNDKKAMKKKVISVKPNQDLYELSNQNEMYRSGFILSGM
                     DTDSQMVSFSNGTQIFQGAEHSQLKEDVQKMQIRRTIGEHLQKEKKLRPLGIKVLSLF
                     FIDKVDNYRPSAQSPTGGKFAKWFEEIYLELAKEEPQGVHNGYFSQDKKGVAKDTNGT
                     TQADNETYQLIMRDKEKLLSLENPLRFIFSHSALKEGWDNPNVFQICTLNDTASEMKK
                     RQEIGRGLRLPVNQQGLRIYDRHLNTLTIIANESYEQFAATLQREIEEDCGVQFAKSN
                     VKKREDKKAVRYRKDFSLHPEFAAIWERIKHQTTYRVDFSNAELIESAVEKIRKMPDI
                     EATKIKHSKVKLEMNETGISTNYRSSNETAVNTQWAIPDLLSEIQKKTGLTRATLCKI
                     LQKSDRLQEVFRNPQRFIDLIAEKINLALQQIMTDGVEYHKIAGKTYEMTLFGDFDFY
                     LNQYTFEVQDKTKTIYEDYIALDSQTENIFARHCESSEQVEFYFKLPKRFKIPTPLGS
                     YNPDWAVVFKGENKIYLVAETKNTGKGIQAGVDEEKLDESERMKIDYARKHFKGLQRD
                     YKDLDYRVVQKVEELSAEYCKI"
     gene            3543..5975
                     /gene="gyrB"
                     /locus_tag="PXE44_001641"
     CDS             3543..5975
                     /gene="gyrB"
                     /locus_tag="PXE44_001641"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL5589141.1"
                     /translation="MSEQTQATSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHM
                     VFEVVDNAIDEALAGHCKDIIVTIHSDNSVSVQDDGRGIPVGIHPEEGVSAAEVIMTV
                     LHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLA
                     IIGNTEKTGTSVRFWPSLDIFKNKTEFEYKILSKRLRELSFLNSGVSIKLIDERDGKE
                     EHFKYEGGIKAYVEYLNEGKTHIHNTPFYLTTEKDGIGVEISLQWNDSYNENVYCFTN
                     NIPQRDGGTHLAGFRGALTRALKNYMDNSGVLKKADANIDASGDDAREGLVAVISVKV
                     PDPKFSSQTKDKLVSSEVRGAVESSMNEALSDYLAENPDDAKNIVTKIIDAARAREAA
                     RKAREMTRRKGALDLGGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAI
                     LPLKGKILNVEKARFDKMLSSQEVATLITALGTGIGRDDYNLEKLRYHKIIIMTDADV
                     DGAHIRTLLLTFFYRQMPELIENGYIYIAQPPLYKVKKGKQERYIQDNDEMSQYEIDI
                     ALEGAGLYVSEDAPALSGVALENLIAQYNGVQKLIERLSRRYPTLLLNELIYSKPLSV
                     EFAKNQANMTAWSEQFVATLMAKEVGGSFYRINTLFNEERQIYEPEIIVTTHGMDSTY
                     RLDYNFINGNEYARIVALGNELNDLLSDSAYVVRGERRQEISSFAEALDWLVKESRKG
                     LTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAADKLFSTLMGDEVEPRRDF
                     IETNALYAQLDI"
     gene            complement(6028..6990)
                     /gene="cmoB"
                     /locus_tag="PXE44_001642"
     CDS             complement(6028..6990)
                     /gene="cmoB"
                     /locus_tag="PXE44_001642"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDL5589142.1"
                     /translation="MIDFRPFYQQIATTDLSAWLETLPLQLKQWEKTTHADYAKWAKV
                     ADFLPNSTACINLKDKVESISSKPLSEGEVKQITHHLKQLMPWRKGPYHLHGIHIDTE
                     WRSDFKWDRVLPHLAPLKDRTILDVGCGSGYHMWRMVGEGAKMVVGIDPTELFLCQFE
                     AVRKLLGNDRRANLIPLGIEQMQPLAAFDTVFSMGVLYHRKSPLDHLSQLKAQLVRGG
                     ELVLETLVIDGDINDVLVPADRYAKMKNIYFIPSVAALINWLEKVGFKNVRCVDEAIT
                     TLEEQRKTDWLENESLVDFLDPNDHSKTIEGYPAPKRAVILANA"
     gene            complement(7087..8850)
                     /locus_tag="PXE44_001643"
     CDS             complement(7087..8850)
                     /locus_tag="PXE44_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDL5589143.1"
                     /translation="MSEQTFITGKDAALEDSIATFQHKLKTLGFNIEEASWLNPVPNV
                     WSVHIRDADCPQCFSNGKGASKKAALASALGEYFERLSTNYFWADFYLGQEIANSEFV
                     HYPTEKWFPIDDEAIPPMEMMDEFLWEYFDPNGEITPELLVDLQSGNYDRGIVTLPYV
                     RQSDNETVYIPQSIIGNLFVSNGMSAGNTKNEARVQGLSEVFERFVKNRIITEAISLP
                     EIPQSVIDGYPTIKASIEKLEQEDFPILCYDASLGGEFPVICVILLNPNNGTCFASFG
                     AHPNFQVAFERTVTELLQGRSLKDLDVFAPPSFNNEDVADHANLETHFIDSSGLISWD
                     LFKRDADYPFVHWDFSGTTEQEFNNMMAIFNKHNQPVYIMDYEHLGVYACRILAPGMS
                     NIYPSDDLVYANNNMGMDWREILLDLPHFHHDAETYQELLDELDEQSIDDLTRVREFI
                     GVVAEKGSAMQTLRIGELKSMLHLALGNLEQASDWANWTANMNASVFSAERNNYYRCL
                     IQSIELFLDEERDSEQYRMVFEKMYGKQAVEAAWGAIQGGNPFHGLTASDESLENMTA
                     HQKLLNAYRKVQKAKVNPIAR"
     gene            9114..11402
                     /locus_tag="PXE44_001644"
     CDS             9114..11402
                     /locus_tag="PXE44_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDL5589144.1"
                     /translation="MEEHTIQSFYAHSGKLSDHSDWQTLLSHANNVGLLAAEFAAPFA
                     SQEIARCTGLLHDLGKYSERYQKRLHGGERVNHSTAGAKIAVERWGPLGKMMAFCIAG
                     HHAGLANGSGEGKNRSTLTQRLQEQFGYGHKDLPVLDAIWQQELNLPEKLPLPPLKPS
                     KNEPFFSYAFFIRMLYSCLVDADFLDTEAFYANLEKRPLERGNYPNLTALHQQFNQFI
                     AQFRERIATQTPNTQEAQRKAEVNRLRSEILDYAVSQAKQETGLFSLTVPTGGGKTFT
                     SMAFALEHAKQYGLCRIIYVIPFTSIIEQNAAEFRKAFGELGTEAVLEHHSTFDHDSL
                     PDKSSRDKLKLASENWDTPVVVTTAVQFFESLFADRSSRCRKLHNIVGSVIILDEAQM
                     LPLNLLRPIMAAIDELARNYQCSVVLCTATQPAIHQANGFYKGFENVREIAPNPTALF
                     EKLKRTTVKHIGIQTDVELQSKLADNPQMLIIVNNRRHARALYDSAKQLEGAYHLTTL
                     MCAKHRSQTLTEIRLRLKQNLPCRVIATSLIEAGVDVDFPLVMRAEAGLDSVAQAAGR
                     CNREGKRLSVESNVWVFQPEEQWKAPPELGLLSGCMRSTVQVHADDLLNPKAINHYFQ
                     AVYEAKGKELDKPNILKACHDAGQSLNFPFQKIAEDFRMITSHLIPVIIPFDQEAENL
                     IDRLRYADQVGGILRKLQPYTVQVPEKALAELYNAGRVETINETNFGKQFYCLIGMDL
                     YDEVAGLSWENVDFLESEKLVL"
BASE COUNT         3585 a         2226 c         2441 g         3180 t
ORIGIN      
        1 gcaggaacac tgctaaaaga attattcgaa acaaaactcc ctgttttctc tgcacctaaa
       61 aatcctaatt ttttaagaca tttaataact ctatttgaag ataaaaattt tatttcttta
      121 gatttctttg ctggcagttc atcatttcca cacgcaatat tggaaagtaa tagaattgat
      181 aagggtaata gaaaatttat tgcggttcaa tatcctgaag aaattgatat caagagtaag
      241 aatggaaaag tagcaaagca attttgccaa agaaaaaatt taccactata tataactgaa
      301 atttcaaaag aacgtattcg ccgtgccgga aagcagattt tgcaaaataa ccccgaaaac
      361 cgaccgcttg atgtaggttt taaagtcttc aaactgacag aaagccattt taaacaatgg
      421 caatcgccca catctgaaaa tttagccgat caacttgaaa tgtttattga tcctgttagt
      481 gaaaaagcgg atacgcaagc cgttttatat gagcttttat tgcgtttagg gttaaaactc
      541 acggcaaaag tgcggttgga aaataaggtg ttttgggtgg aagaaaacgg gctgattttc
      601 gccttgctgt taaatgcagc cgacgaagaa attattcaaa ctgttatcgc tcaacaaccg
      661 aaaaaagtca tcgctttaga ccgtttattc aacggcaatg atgcccggaa gaaaaacacc
      721 gaattgcaaa tgcaagatgc cggcattacg ttttttgtga tttaaggaga aaaaatggaa
      781 ctccaatttg aaaccttaga gtatcaatta caagccgtta atgccacagt aaatttgttt
      841 gtcggacagc cgaatgccgc aactgaattt tcactcaaag cccaaaatga tatgcggttt
      901 gtgccgaatt taggcttgca gattagtgat gagcagttac aacagaattt ggctaatctg
      961 caaaacaggc aaaaaatcga ccgcactttg cttgtcgaac agggaaaaaa ttttaccgtg
     1021 gaaatggaaa ctggtacagg gaaaacctat gtctatttac gcacgatttt tgaattaaac
     1081 cgtcaatacg gctggcaaaa atttgtgatt gtcgtgccga gtgtggcaat tcgtgaggga
     1141 gtgttgcata cgttggaaac tacaaaaagc cattttaata cggtatttga taacccgagc
     1201 gtcaatcaaa aatttgaata taaaagtaac caaacttcac gcttaaaatc ctttgctagt
     1261 gctaatcata ttgaaatttt agtgatgaat attgatgctt tcaccaaaga aagtaacgtg
     1321 attaataccg tgaatgaaag cggtgatgct ccgattttct atattcaaca ggcgaatccg
     1381 attgtgatta ttgatgaacc gcaaaatatg gaaacggaga ttcgccgtaa tgcgattgag
     1441 agtttaagtc cgcttttcac tttgcgttat tctgccaccc ataaaaatgc gtataacccg
     1501 atttttagcc tcaatccggt gcaagcctat gaaatgggat tagttaaaca aattgaggta
     1561 gatagcgtat tggcggaaaa tgaggtaaat ggagcttatc tttccctcaa agagattaaa
     1621 acaggggcaa aaagctggtc ggcaaaaatc gaaattttat ttaacgataa aaaagcgatg
     1681 aaaaagaaag tgattagcgt gaaaccgaat caggatttgt atgagctttc caaccaaaat
     1741 gaaatgtatc gttcagggtt tatattaagc ggaatggata ccgatagcca aatggttagc
     1801 ttctccaacg gcacacaaat ttttcaaggg gcggaacatt cccaacttaa agaagatgtg
     1861 caaaaaatgc agattcgccg cactattggc gagcatttgc aaaaagaaaa gaaattacga
     1921 ccgcttggga tcaaggtgtt gtctttgttc tttatcgata aagtggataa ttatcgccct
     1981 tctgcacaaa gccctacggg gggaaaattt gcaaaatggt ttgaggaaat ttatttggag
     2041 ctagccaaag aagagcctca aggcgtgcat aacggttatt tctcacagga taaaaaaggc
     2101 gtagcaaaag atactaatgg cacaacgcaa gcggataacg agacttatca actgattatg
     2161 cgggataaag aaaaattgct ttcgctggag aatccgttac ggtttatttt ctcgcattct
     2221 gccttaaaag aaggatggga taatccgaat gtgtttcaaa tttgtacttt aaacgatact
     2281 gcaagcgaaa tgaaaaaacg ccaagagatt gggcggggct tgcgtttgcc tgtgaatcaa
     2341 caaggattgc ggatttatga tcgtcatcta aatacgctga caattatcgc aaatgaaagt
     2401 tacgaacaat ttgccgccac attacagcgt gaaattgagg aagattgtgg tgtacagttt
     2461 gccaaaagca atgttaaaaa acgggaagat aaaaaagccg ttcgctaccg taaagatttc
     2521 agcttacatc cggaatttgc ggcaatttgg gaacggatta aacaccaaac cacttatcgg
     2581 gtagatttca gtaatgcgga attgattgaa agtgcggtgg aaaaaatccg caaaatgccc
     2641 gatatcgagg cgacaaaaat taagcattcc aaagtgaaat tggaaatgaa tgaaacaggg
     2701 atttcaacta attatcgctc atctaatgaa acagcggtaa atacacaatg ggcgatacct
     2761 gatttattga gtgagattca aaagaaaacc gggctgacgc gtgcgaccct ttgcaaaatt
     2821 ttacaaaaat ccgaccgctt gcaagaagtc ttccgcaatc ctcaacgctt tattgattta
     2881 attgcagaga aaatcaacct tgctttacaa caaattatga cagacggggt ggaatatcac
     2941 aaaattgccg gaaaaaccta tgaaatgacg ttattcggcg attttgattt ttacctcaat
     3001 caatatactt ttgaagttca agataaaaca aaaacgattt atgaagacta tattgcactg
     3061 gattcccaaa cagaaaatat cttcgcccga cattgtgaat ctagtgagca agtggagttt
     3121 tattttaaat tacccaaacg ttttaaaatt ccaacgccat taggcagcta taatcccgat
     3181 tgggcagttg tgtttaaggg agaaaataaa atttacttgg ttgccgaaac gaaaaataca
     3241 ggcaaaggta ttcaagcagg agtagatgaa gaaaaattag atgaaagtga acgaatgaaa
     3301 atcgactatg cccgtaagca tttcaaaggc ttacagagag attataagga tttggattat
     3361 agggttgtac agaaagtgga ggagctgtct gcggagtatt gtaaaattta atctgtggtc
     3421 ttgtagaatt gagatagact gcaatttcaa ctaaaattta accgcttgtt ttgcgatact
     3481 tttacgcatt tttctgctat aatcggcaca attttttcga tttcaaatta gtaggaataa
     3541 gaatgtctga acaaactcaa gcaacaagtt atgattcttc cagtattaag gtgttacgcg
     3601 gcttagatgc ggtgcgtaaa cgcccgggaa tgtatatcgg cgataccgat gacggtacgg
     3661 gcttacacca tatggtgttt gaggtggttg ataatgcgat tgacgaagca ttagccgggc
     3721 attgtaaaga cattattgtc acaattcatt ccgataattc ggtttctgtg caagatgacg
     3781 gtcgtggaat tccggtgggg attcacccgg aagaaggcgt ttcggcagca gaagtcatta
     3841 tgactgttct tcacgcaggc ggtaaatttg acgataactc ctataaggtt tccggcggtt
     3901 tgcacggtgt gggcgtatcg gtggtgaatg cactttcctc taaattacaa ctcactattc
     3961 gccgtgaagg acacgttcac gagcaattct atagcttagg tgagcctgat gcacctttag
     4021 cgattatcgg caataccgaa aaaacgggga cgtcggttcg tttctggcca agtttggata
     4081 ttttcaaaaa taaaaccgaa tttgaatata aaattctgtc taaacgcttg cgtgaactat
     4141 catttttgaa ttcaggcgtt tcgattaaat taattgatga acgagacggc aaagaagagc
     4201 attttaaata tgagggcggt attaaagcct atgttgagta tttaaacgaa ggcaaaactc
     4261 atattcacaa tacgccattc tatttgacca ctgagaaaga cggcattggc gtggaaattt
     4321 ccctacagtg gaatgacagt tacaacgaaa atgtgtactg ctttaccaat aatattccac
     4381 agcgtgatgg cggtacgcac ttagccggtt tccgtggggc attaactcgt gcgttgaaaa
     4441 actatatgga taacagcggg gtattgaaaa aagccgatgc gaacattgat gcctcaggtg
     4501 atgatgcccg tgaaggttta gtagcggtga tttcggtgaa agtgccggat ccgaaattct
     4561 cctcacaaac caaagataaa ttggtgtcgt cagaagtgcg tggagcagtg gaaagctcaa
     4621 tgaatgaggc tttatccgac tatttagccg aaaatccgga cgatgcaaaa aatatcgtta
     4681 ccaaaattat tgatgcagcc cgtgcaagag aagcagctcg taaagcccgt gaaatgaccc
     4741 gccgtaaagg ggcgttagat ttaggcggct taccgggtaa attagctgat tgtcaggaaa
     4801 aagatccggc gttatcggag ctttacttgg tggagggtga ctctgccggt ggctcggcaa
     4861 aacaagggcg tgaccgtaaa aaccaagcga ttttgccgtt aaaagggaaa attctgaacg
     4921 tggaaaaagc ccgctttgat aaaatgcttt cttcacaaga agtagcaacc ttgattaccg
     4981 ctttaggcac aggcatcggg cgtgatgatt ataatttgga aaaattacgt taccacaaaa
     5041 tcattattat gaccgatgcg gacgtggatg gtgcacatat tcgtacgctt ttattaactt
     5101 tcttctaccg tcaaatgccg gagctgattg aaaacggcta tatttatatt gcacagccac
     5161 cgctttataa agtgaaaaaa ggcaagcaag agcgttatat tcaagataac gatgaaatgt
     5221 cgcaatatga aattgatatt gcattagaag gtgcaggctt atatgtgagt gaagatgctc
     5281 cagcgttaag tggcgtagcg ttagagaact taattgccca atataacggc gtacaaaaat
     5341 taattgaacg tttaagccgt cgttacccaa cattattact taacgaatta atttattcaa
     5401 aaccgctttc ggtggaattt gcaaaaaatc aagcaaatat gaccgcttgg agcgagcaat
     5461 ttgtcgctac attgatggca aaagaggtgg gcggcagttt ctaccgtatt aataccttgt
     5521 tcaatgaaga acgccaaatt tatgagccgg aaattattgt gactacacac ggtatggaca
     5581 gtacctatcg tttagattac aactttatca acggtaatga atacgctcgc attgtggcat
     5641 taggcaacga gctgaacgat ttactatctg attcagccta tgtggtgcgt ggtgaacgtc
     5701 gccaagaaat cagcagtttt gctgaagcct tagattggtt ggtgaaagaa tctcgtaaag
     5761 gcttaactat tcaacgttat aaagggttag gtgagatgaa tccggaacaa ttatgggaaa
     5821 ccacgatgga tccggtggct cgtaaaatgc tacaggtcaa tattaccgat gcgattgcgg
     5881 cagataaact cttcagtacg ctgatgggcg atgaagttga accacgccga gatttcattg
     5941 aaactaacgc tttgtatgct cagttagata tttagttaaa aaatccgcat ttgacatgcg
     6001 gattttttat tctcttaaac tagcatttta agcattcgct aaaatcaccg cccgcttcgg
     6061 agcagggtag ccttcaatgg ttttgctatg atcgtttgga tctaaaaaat ccactaaact
     6121 ttcattttcc aaccaatcgg tttttcgctg ctcttctagt gtagtgattg cctcatcaac
     6181 acaacgcaca tttttaaagc cgactttttc cagccaatta atgagtgcag caacagaagg
     6241 aataaaatag atatttttca ttttcgcata gcgatcagcc ggcactaaaa catcattaat
     6301 atcgccatca atcaccagcg tttctaacac caattcaccg cctctgacta attgggcttt
     6361 gagttgagaa agatgatcaa gcggtgattt gcgatgataa agcaccccca ttgaaaacac
     6421 ggtatcaaaa gcagcaagcg gttgcatttg ctcaatacca agtggaatca gattagcccg
     6481 tctatcattc cctaagagtt tccgcactgc ctcaaattgg cacaggaaca gctcggtcgg
     6541 gtcaatacca actaccattt ttgccccttc gcctaccatt cgccacatat gataaccgct
     6601 accgcagccg acatctaaaa tggtgcggtc ttttaacggt gcaagatgcg gaagcacacg
     6661 atcccattta aaatcggaac gccattcggt atcaatatga atcccgtgta gatgataagg
     6721 gcctttacgc cacggcatta gttgctttaa atggtgggta atttgcttga cttccccctc
     6781 agataaaggc ttgcttgaaa tagactcaac cttgtctttt agattgatac aagcggtcga
     6841 atttggtaaa aaatctgcaa cttttgccca cttggcatag tcagcgtggg tggtcttttc
     6901 ccattgtttg agttgtaagg gtaaggtttc taaccaagca gaaaggtcgg ttgtggcaat
     6961 ttgttgataa aaaggacgaa agtcaatcat tttgaaattt atgtctaggg gagagaccta
     7021 tgccctcccg aaaggtagta aatagaaagg acaggcacag aacctacccc tacgaaaaat
     7081 ataaaattat ctcgcaatcg gatttacttt cgctttttgc actttgcgat aagcgttcag
     7141 taatttctga tgtgcagtca tattttcaag cgattcgtcc gatgcagtta aaccgtggaa
     7201 cgggttaccg ccttggattg caccccaagc agcttcaacc gcttgtttac cgtacatttt
     7261 ctcaaacacc atacgatatt gttccgaatc acgctcttca tctaagaaca actcaatcga
     7321 ttggattaag cagcggtaat aattgttacg ttctgctgag aatacgcttg cattcatatt
     7381 cgctgtccag tttgcccagt ctgaggcttg ctctaagtta cctaaagcta aatgcagcat
     7441 cgatttaagc tcaccgatac gcaatgtttg cattgcagag cctttctctg ccaccacgcc
     7501 aataaattca cgcacacggg ttaaatcatc aatgctttgc tcatctaatt catcaagcag
     7561 ttcttggtaa gtttcagcat cgtgatggaa atgtggcaaa tctaacaaga tttcacgcca
     7621 atccataccc atattgttat tggcatagac caaatcatcg cttggataga tattcgacat
     7681 acccggtgca agaatacggc aagcgtacac acctaaatgc tcataatcca taatatacac
     7741 aggctgatta tgcttgttga aaatcgccat catattatta aattcttgct ccgttgtgcc
     7801 ggagaagtcc caatgcacga acggataatc cgcatcacgt ttgaaaagat cccacgaaat
     7861 tagaccgctt gaatcaataa agtgtgtctc aaggttcgca tgatctgcta catcttcatt
     7921 attaaatgaa ggtggggcaa acacatctaa atcctttaag ctacggcctt gtaataactc
     7981 ggttacggta cgttcaaacg ccacttggaa attcgggtgt gcaccaaaag aggcaaaaca
     8041 agtaccgtta tttgggttta acaagatcac acaaataact ggaaattctc cgcctaatga
     8101 agcgtcatag cataaaatcg ggaagtcttc ttgctctagt ttctcgatag aggctttgat
     8161 tgtcggataa ccgtcaatca cactttgcgg aatttccggc aagctgattg cctcagtaat
     8221 aatacggttt ttgacaaaac gctcgaacac ttcggataag ccctgcactc gagcttcatt
     8281 ttttgtgttg cctgccgaca taccgtttga aacaaacagg ttgccgataa tgctttgcgg
     8341 aatgtaaacc gtttcattgt cagattggcg aacgtaaggt agggtaacaa ttccacgatc
     8401 ataatttcct gattgtaaat caaccaataa ttccggcgtg atttcaccat tcggatcaaa
     8461 atattcccat aaaaactcgt ccatcatctc cattggtgga atggcttcat catcaatcgg
     8521 aaaccatttt tcggttggat aatgcacaaa ttcactatta gcaatctctt gacctaaata
     8581 aaaatccgcc cagaaatagt tggtagataa acgctcaaaa tactcaccta atgcagaggc
     8641 aagagcggct tttttgcttg ctcccttacc gttagaaaaa cattgcggac agtctgcatc
     8701 acgaatatgc accgaccaca cattcggaac aggatttaac caagaggctt cttcaatatt
     8761 aaaacctaag gtttttaatt tatgctgaaa ggtcgcaatg ctgtcttcaa gtgcagcatc
     8821 tttaccggta ataaaagttt gttctgacat gataatacct ttaacttgtc gtaaaaatgg
     8881 caacattcta gcaaaaatcc ccatagataa gtaaattttt tgtgaaattt ctgctgaaag
     8941 aacagatttc aaaacttaga aaaaatgatt acaagcggtc aaattttact tgaattttgc
     9001 aagtgaaatt tgtgtttgat ttagattaag aaaatattat ctaggatata tacagaataa
     9061 aaggttagag gtagtattgc ttgtgttagg aaggaaatat tgaaggtata aaaatggaag
     9121 aacatactat tcagtctttt tatgcccatt cggggaagct ttcagatcat tcagattggc
     9181 aaactttact cagccacgcc aataatgttg gcttgcttgc cgccgaattt gctgcgccgt
     9241 ttgcttcaca agaaattgct cgctgcacgg ggctattgca cgatttaggc aaatatagtg
     9301 aacgttacca aaagcgttta catggcggag aacgggtaaa tcactctact gctggtgcaa
     9361 aaattgcagt ggaacgttgg gggcctttgg gcaaaatgat ggcgttttgt atcgcaggcc
     9421 accacgctgg gttagcaaat ggttctggag aagggaaaaa tcgttctaca cttacacaac
     9481 gtttgcaaga gcaatttggc tatggtcata aagatctacc ggtacttgac gctatttggc
     9541 agcaagaact taatttgcct gaaaaattgc ccttaccgcc gttaaagcct tctaaaaacg
     9601 aacctttctt ttcttacgcc ttttttatcc gaatgctcta ttcttgcctt gtcgatgccg
     9661 actttcttga tacagaagct ttttatgcca atctcgaaaa aagaccgctt gaacgaggaa
     9721 attaccccaa tttaaccgca ctccatcagc aatttaatca atttattgca cagtttaggg
     9781 agcgaattgc cactcaaacg cctaatacgc aggaagcaca acgtaaagcg gaagtaaacc
     9841 gcttgcggag tgaaattttg gattatgccg tatctcaagc aaaacaggaa acaggtctgt
     9901 tttctctgac tgtgccaacg ggcggtggta aaacctttac ttcaatggcg tttgcccttg
     9961 agcatgctaa acagtatggt ttatgccgca ttatttatgt tattccgttt accagcatta
    10021 ttgaacaaaa tgctgcggaa tttcgtaaag cttttggcga attaggaaca gaggcggtat
    10081 tggaacatca cagcactttt gatcacgata gcctacctga taaatcaagc cgagataagc
    10141 tgaaattggc atcagaaaat tgggatactc ctgttgtggt tacaactgca gtgcaattct
    10201 ttgaaagttt gtttgccgac cgttcttcac gctgtcgcaa attgcataat atcgtcggta
    10261 gcgtgattat tttggacgaa gcccaaatgc taccgttgaa tttattacgc ccaattatgg
    10321 ctgcgattga tgaattagct cgcaattatc aatgttcggt agtgctttgc acagcgacac
    10381 aacccgctat tcatcaagcg aacggttttt ataaaggatt tgaaaatgtg cgagaaattg
    10441 cccctaatcc aaccgcactt tttgaaaaat tgaaacgtac tacggtaaag catatcggta
    10501 ttcaaacaga tgtggagttg caaagcaaac tcgcagataa cccacaaatg ctgataatcg
    10561 tgaataaccg ccgtcacgcc cgagcgttat atgattcagc gaaacagtta gagggagctt
    10621 atcacctcac cacgctaatg tgtgctaaac accgctcaca aacgctgact gaaattcgtc
    10681 ttcgcctaaa acaaaaccta ccttgccgag tgattgccac ttctttaatt gaagccggtg
    10741 ttgatgtcga tttcccactg gttatgcgag ctgaagcagg actagattca gtggctcaag
    10801 cagcagggcg ttgcaaccga gaggggaaac gtttatctgt agaaagcaat gtttgggttt
    10861 tccaacctga agaacagtgg aaagcaccac cagagctagg cttactttct ggctgtatgc
    10921 gttcaacggt tcaggttcac gcagatgatc tacttaatcc gaaagcaatc aatcattatt
    10981 tccaagcggt ttatgaggct aaaggaaaag agctggataa gccaaatatt ttaaaggcgt
    11041 gtcacgacgc tgggcaaagc cttaatttcc cattccaaaa aatagctgaa gatttccgaa
    11101 tgatcacaag ccatttaatt ccggtgatta tccctttcga ccaagaagcc gaaaacctta
    11161 ttgaccgttt acgctatgcg gatcaagtag gcggtatttt aagaaaactc cagccttaca
    11221 ctgtgcaagt accggaaaaa gcattagccg aactctataa cgctggcaga gttgaaacca
    11281 ttaatgaaac caacttcggc aaacagtttt actgcttaat tggaatggat ttgtatgatg
    11341 aagtggcggg gttgagttgg gagaatgtgg attttcttga gtcggagaaa ttggttttat
    11401 agatgaatgt ttattttaac ttctttataa gt
//
LOCUS       DANLZC010000082        11411 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.082, whole genome shotgun sequence.
ACCESSION   DANLZC010000082 DANLZC010000000
VERSION     DANLZC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11411
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.082"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            262..2058
                     /locus_tag="PXE44_001645"
     CDS             262..2058
                     /locus_tag="PXE44_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate-specific subunit IIBC"
                     /protein_id="HDL5589145.1"
                     /translation="MDFLTEILLFLKDQVLNKAPFLLGIVACLGYILLKKDTSTIIKG
                     TIKTIVGFMIVQAGSGFLVTNFKPVIDGLAKYHQLTGAVIDPYTSMQSTIQTMGENYA
                     WVGYAVIGALFLNILLVVFRRLTGIRTIMLTGHIMFQQAGLVAVFYMIIGASMWETII
                     YTSVLMALYWGISSNIMYKPTQAVTGGAGFSIGHQQQIASWIAVKLAPKLGDKDDTVD
                     KMKLPNWLNIFHDSISATALVMTIFFGIILLSFGLDNLKELAGKTNQFMYILEMGLKF
                     AVAIQIIVTGVRMFVAELSEAFKGIAERLIPNSVLAIDCAAIYAFSPNAMVFGFMWGA
                     IGQFVAVGGLLAVGSPVLIIPGFIPMFFSNATIGVFANQFGGWKAVMKICFVMGIIEV
                     LGSAWVIQMLASQGTTFNGWMGMADWALFFPPILQGIISIPGFFFVIVAAAMVYMFFA
                     SKQLRAEEAEAAARGITLEQLDGYGLDEVKAEPKAEAVVQQAVENAENSANPIRILAV
                     CGSGQGSSMMMKMKIKGYLDKRGIPNIMDSCAVTDYKGKLSEVDIIVSSKHLANEIEV
                     PEGKAVLGVQNMLNPNSFGDELVELINQHNAK"
     gene            2138..3199
                     /locus_tag="PXE44_001646"
     CDS             2138..3199
                     /locus_tag="PXE44_001646"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5589146.1"
                     /translation="MKKILSTLFMLSAVSVAVAKEVNIKLLGTSDVHGRVVPWSYGAD
                     VEDKSGSFAQIATYVKEVRANHKNVLLFDIGDAIQDNQVEEFAKTLDKAKNNPVPKVL
                     NAMNYDIFILGNHEFNFGMPNLDAILKDINAKILTANFYHKDGKRYVEPTTIIERDGV
                     KLGIIGLTTPMSAIFEKDTGHLDAMKFTSPIEEAKAQIADLKAKGVDAIIVLAHMGIE
                     NENNIPDTGVADLVNAVDGIDVIIAGHMHKDVPSETIKNTLITEPHRYGTVVSEVDLA
                     FDVNDKGEVKLLSKTAKTVPVKKLASDPEIEKIYQPYHNELRALNNVKIGETAQDLIP
                     QGKITVYRLLSRKTPECPH"
     gene            3187..3651
                     /locus_tag="PXE44_001647"
     CDS             3187..3651
                     /locus_tag="PXE44_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDL5589147.1"
                     /translation="MSSLIHDVQQHYSGADVVSFAFDYETVRLDKGDIKKKDIIYNYR
                     YAGGDVSMYELTGKQLKQYMEWSADYFDTIQAGDKDYRYNAVRGKSKYVTFDLFGGVS
                     YNIDLRNPSGSKIVDLKLANGSLITDDGKYKVGMNSYRFGQLTKKGGIWEGQ"
     gene            3682..3798
                     /locus_tag="PXE44_001648"
     CDS             3682..3798
                     /locus_tag="PXE44_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser/Thr protein phosphatase"
                     /protein_id="HDL5589148.1"
                     /translation="MGAEKGTIQNMLIDYITNVKGGKIEGKSHNRWAIIGLN"
     gene            3814..4281
                     /locus_tag="PXE44_001649"
     CDS             3814..4281
                     /locus_tag="PXE44_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5589149.1"
                     /translation="MNLKQSLIENNSILLNQSAANWEDAIKIGTDLLVKAGTIEPRYY
                     DNIVSKIKEMGPYIILAPGLAMPHARPEEGVIKTSFALVTFDTPIYFEGEDEPVHVML
                     TLAGSDSDQHMQGLVEITQVLDDPESDTGVDLDKIRRCKTAEEVYAVIDQALA"
     gene            4336..5016
                     /locus_tag="PXE44_001650"
     CDS             4336..5016
                     /locus_tag="PXE44_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL5589150.1"
                     /translation="MSKPLLQIALDSLSLEKAVADAKQAENQVEIIECGTILAFAEGM
                     KAVSTLRVLHPNHIIVCDLKTTDGGAILAKMAFEAGADWLTVSAAAHPATKAACKKVA
                     DEFNAAHPELKVKKEIQIEIYGNWTVEKDAKEWVELGVTQAIYHRSRDAELAGKGWTT
                     EDVELMKQLSDLGIELSITGGIVPEDIHLFKEIKNAKAFIAGRALVGEKGKQTAEAIR
                     VEIDKYWN"
     gene            complement(5084..6226)
                     /locus_tag="PXE44_001651"
     CDS             complement(5084..6226)
                     /locus_tag="PXE44_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL5589151.1"
                     /translation="MKIVIAPDSFKESLTALEVAQAIQQGFARVFPEAEYQLVPMADG
                     GEGSVQSLVDATHGSLQKISVIAPLANEVEGFFGLSGDQKTAFIEMAAASGLHLVPME
                     QRNPLVTTSYGTGQLIKAALDLGVEKIILGIGGSATNDGGVGMLQALGAKFLNAEQRE
                     IGFGGEALSQIQQIDLSGLDSRLKQVEFEVACDVDNPLCGERGASAIFGPQKGATPEM
                     VKMLDHALLHFAETVKSQLGIEIAEKAGAGAAGGMGGGLLLLPNVTLRSGVQIILEAT
                     DLAEKLADADLVVTGEGRMDAQSIAGKTPIGVAKVAKQFGKPVIAIVGSLKEDYPVVY
                     QHGIDAVFPIIRQVSNLEAVLKSGKENLISTAENVARLWKISALQE"
     gene            complement(6236..7615)
                     /locus_tag="PXE44_001652"
     CDS             complement(6236..7615)
                     /locus_tag="PXE44_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL5589152.1"
                     /translation="MSGIALILIFLVAIIIMIYMIAKWKVHPFLALMSISIALALVAG
                     LPLAKIPTIVGEGFSNTFKSIGIVIIFGAIIGTILEKTGAALKLADMVVKVVGQKRPE
                     LAMLIMGWVVGIPVFCDSGFVVLNPIREAIRKKILANPVGMAVALSAGLYASHVFIPP
                     TPGPIAAAGAVGLSHNLLLVIGMGFVVSIPVLIASYFFAKYVGKQVSVMEEAQADQVI
                     SQSYEDLIKQYGKLPSGFLSFAPIFMPIILMAIGSIAKIAGVSGDFGVFLQFVGNPII
                     ALAIGVIFAIFLLVSSGKMAEFDSLTNDTLKLVGPILFITAAGGVLGNVITQAGFVDY
                     IKQNAHIISTAGIFFPFLISAILKTAQGSSTVAIITTASIMGMFNAPDSLMNALGLTS
                     EMAAALTVMAIAAGSMCVSHANDSYYWVVTNFTKLTPQQGYRTQTTLTFIMGVVGMLT
                     VYLLSLILL"
     gene            complement(7737..8831)
                     /locus_tag="PXE44_001653"
     CDS             complement(7737..8831)
                     /locus_tag="PXE44_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5589153.1"
                     /translation="MKLNQAIADRIVKRTMQIIPNSVNVMDSEGVIIASGDVGRIGQR
                     HTGAVIALRNDQAVEIDENLAKQWCDEVKAGINLPLHYLGSTIGVVGISGQPQQVRQY
                     AQLVKMAAELIMQQAFELEQERWQRRYREEFVRALLKGSLSTAEIQQQAVFFGQDFAK
                     PLSVMVIKIHGSTAEKLQRLIDFFEHYFPSLLTAVIGLDKIALLNHLSNEKSLIRNAL
                     NKLNDPDISFKTVVGLAVENLSQTHISYQTACHTLNYAEQIQSRKQIIHFEDFKLPAL
                     LHSFSHSWEAKTLLSPFQALLVSDKKQVLLKSLRYYFFANCDLDHAAKKLFIHPNTLR
                     YRLEKIEQITGLSFNNIEQKFVLYLGAVLS"
     gene            complement(8935..9387)
                     /locus_tag="PXE44_001654"
     CDS             complement(8935..9387)
                     /locus_tag="PXE44_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HDL5589154.1"
                     /translation="MNLKAVAVSALAMSSVAMADIADVKPVVFESKFDFQTTVTTLTN
                     TLKEKGMTIFAEIDHQAAAKQAGLEMQPATVLVYGTPKAGTPLMVKDPMLALQLPLKV
                     LITEPNKGKVEVMLNRADQVVAHANTDYADVENNLAKAEKLIKATVAK"
     gene            complement(9408..10160)
                     /locus_tag="PXE44_001655"
     CDS             complement(9408..10160)
                     /locus_tag="PXE44_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL5589155.1"
                     /translation="MHFEKRSHWSGELGREMSFNVYGHAGKPVIVFPSSGGNENEYGN
                     FGMIEACRSFIERGLIKFYTPDSYDAESWLSKHKSGHDMALAHNAYDRYIIHELVPLI
                     RHESHWNGTMIATGCSMGAFHTVNFALRHPDLFDIAIALSGVYDARFFSGEFYGDKAV
                     YFNSPIDYVWGQNDDWFLDRYRQNHYVIAVGQGAWEEQHIEDTRRLQEAFNAKGIDAW
                     FDYWGFDIDHDWPTWRKQMPYFLGKLEEQGII"
     gene            complement(10171..11355)
                     /locus_tag="PXE44_001656"
     CDS             complement(10171..11355)
                     /locus_tag="PXE44_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL5589156.1"
                     /translation="MSRPLNFVMISPHFPTNFETFAIRLREAGFNTLGIADAPYESLS
                     ENLRHSLTEYYRVDNMEDYEQVYRAVAYFAHKYGRIDRIESHNEYWLELDAKLRTDFN
                     VFGYKSDDMLSIKTKAQMKEVFRKTGLKVAQGRVFKDDEDARKLAKQLKFPVIIKPNS
                     GVGASDTYKIKTAEELEAFFGYKTPTVEYIMEEFIDGDIITFDGLTDKEGNIVFYSSL
                     EYSEAVLDTVSKDGDMFYYVPCEISPKLVEMGKQCVKAFNVKERFFHFEFFRVKGSKK
                     EELLPLEINCRPPGGLTIDMWNYANDFDVFREFANIVKENKFYADIQHPWNVVYISRK
                     ANQNYVNSIEAVCEKFAGNIISVQSVPGVFAKVMGEQGILARTETMEQMREIAQFAQA
                     KQ"
BASE COUNT         3300 a         2606 c         2370 g         3135 t
ORIGIN      
        1 tactggtaaa aatttatttt aataacttga tttaaatacc atttatttag catatatgag
       61 ctaatttaaa gaataatctt taataatcac gaataaatat atttttcttc ctccatgacg
      121 agattataaa aataaaatgt gatctcgctc acaaataatt aaaaataatc ttttttaatc
      181 tttttctagc gtttatcatt aattccgaca acttctctgg agttttttag ttctattaat
      241 ctctcaacaa agaggccccc tatggacttt ttaaccgaaa ttctcctttt cttaaaggat
      301 caagtgctga ataaagcacc cttcttacta ggtatcgtag cttgcttagg ttacattctt
      361 cttaaaaaag acacttctac gattattaaa ggcacaatta aaacaattgt cggttttatg
      421 attgtacaag ccggttcagg tttcttagta accaacttta agccggttat tgatggctta
      481 gccaaatatc accaacttac cggtgcagta attgaccctt acaccagtat gcaatcgacc
      541 attcaaacca tgggcgagaa ctacgcttgg gttggttatg ccgtaattgg ggcgctgttc
      601 ctcaatattc tattagtggt gttccgccgt ttaaccggta tccgtaccat tatgctcacc
      661 ggtcacatta tgttccaaca agcaggcttg gttgcggtgt tctatatgat catcggagcg
      721 tcaatgtggg agaccattat ctacacctca gtgttgatgg ctctttattg gggtatttcg
      781 tctaatatta tgtataaacc aacccaagcg gtaacaggcg gtgcaggctt ctcaattggt
      841 caccaacaac aaattgcttc ttggattgca gtcaaactcg caccaaaact tggcgataaa
      901 gacgatactg tagataaaat gaaattgcct aactggctca atattttcca cgacagtatt
      961 tctgcaaccg cattagtaat gaccatcttc tttggaatca tcttactttc attcggttta
     1021 gataacctca aagaacttgc tggcaaaacc aaccagttta tgtacattct tgaaatgggc
     1081 ttgaaatttg cggtggcaat tcaaatcatt gtaaccggcg tacgtatgtt tgtagctgag
     1141 ctttcagaag ccttcaaagg tattgctgaa cgcttaattc caaactcagt attagcgatt
     1201 gactgtgccg ctatctacgc tttctcgcca aatgcgatgg tattcggctt tatgtggggg
     1261 gctatcggtc aatttgtggc agtaggcggc ttgcttgcag taggctcacc ggtgcttatt
     1321 attccgggct ttatcccgat gttcttctct aacgcaacca ttggtgtgtt tgcaaaccaa
     1381 tttggtggtt ggaaagcagt aatgaaaatc tgttttgtga tgggtattat tgaagtgctt
     1441 ggctcagctt gggttattca aatgcttgct tcacaaggca caacctttaa cggttggatg
     1501 ggtatggctg actgggcatt gttcttccca ccaatcctac aaggtattat cagcattccg
     1561 ggcttcttct ttgtaattgt ggcagcagca atggtttata tgttcttcgc atctaaacaa
     1621 cttcgtgccg aagaagcaga agcggcagct cgtggcatta cacttgaaca attagacggc
     1681 tacggcttag atgaagtaaa agcagaacca aaagcggagg cagtagtaca acaagcggtc
     1741 gaaaatgccg aaaattctgc aaacccaatc cgcattctag cggtgtgtgg ctctggtcaa
     1801 ggctcatcaa tgatgatgaa aatgaagatc aaaggctatc tcgacaaacg tggtattccg
     1861 aatattatgg attcctgtgc ggtaactgat tacaaaggca aattaagcga ggttgatatt
     1921 attgtctcca gcaaacattt agccaacgaa atcgaagtac cggaaggcaa agcggtatta
     1981 ggtgtacaaa atatgctaaa tccaaactca tttggtgatg aattagttga gctaatcaat
     2041 cagcataatg caaaataagt aagtttaggg gtgaataatc acccctatct atgctaaaga
     2101 ttgtaaaaac tcacttcatc aaacgaggaa ttatccaatg aaaaaaatct tatccaccct
     2161 gtttatgctc tccgcggtta gcgttgccgt tgccaaagag gtaaacatta aactacttgg
     2221 tacctcagac gttcacggac gtgttgtgcc gtggagctac ggggcagacg tggaagacaa
     2281 atccggctca ttcgcccaaa ttgcaaccta tgtcaaagag gtgcgtgcca accacaaaaa
     2341 tgtgttgctg tttgatattg gcgatgcgat tcaagacaac caagtcgaag agttcgctaa
     2401 aaccttagac aaagcgaaaa acaatcccgt gccgaaagtg ctgaatgcaa tgaattacga
     2461 catcttcatt ctcggcaacc acgaatttaa cttcggaatg ccgaatttag atgcgatttt
     2521 aaaagacatc aacgccaaaa ttctcactgc caacttctat cacaaagacg gcaaacgcta
     2581 cgttgagcca actaccatca ttgaacgtga tggcgtaaaa cttgggatta tcggcttaac
     2641 caccccaatg tcggctattt ttgaaaaaga caccggtcat ttagacgcaa tgaagttcac
     2701 ttcgccgatt gaagaggcga aagcccaaat tgccgatctc aaagccaaag gcgtggacgc
     2761 cattattgtg ctggcacata tggggattga gaacgaaaac aacattcccg acaccggcgt
     2821 ggcggattta gtgaatgcag tggacggtat tgatgtaatt atcgccggcc atatgcataa
     2881 agatgtgccg agtgaaacta tcaaaaatac cctgattacc gaaccgcacc gctacggcac
     2941 agtggtttcc gaagttgatc ttgcctttga tgtgaatgac aaaggcgaag tgaaactact
     3001 ttctaaaacc gccaaaaccg taccggtgaa gaaactcgca agcgatccgg aaatcgaaaa
     3061 aatttaccaa ccgtatcaca atgaactgcg tgccttaaac aacgtcaaaa tcggtgaaac
     3121 tgcacaagat ttaatcccac aaggcaaaat cacggtgtat cgattgcttt ctcgcaagac
     3181 accggaatgt cctcattgat ccacgatgtg caacaacatt acagcggtgc agacgtagtg
     3241 agttttgcat ttgactacga aaccgtgcgt ttagacaaag gcgacatcaa gaaaaaagac
     3301 atcatctaca actaccgcta tgccggtggc gatgtgagca tgtatgagct aaccggcaaa
     3361 cagcttaaac agtatatgga atggtcagcg gattacttcg acaccattca agccggtgac
     3421 aaagactacc gctacaacgc tgtgcggggt aaatctaaat acgttacctt cgacctattc
     3481 ggcggcgtaa gctacaacat cgacctacgc aatccaagcg gtagcaaaat tgtcgatcta
     3541 aaactggcaa acggcagtct aatcaccgat gacggcaaat acaaagtggg aatgaactcc
     3601 taccgctttg gtcagctcac caaaaaaggc ggcatctggg aaggacagta aatccccgtg
     3661 atttgggaat ccaaagtcgc catgggggct gaaaaaggca caatccaaaa tatgctgatt
     3721 gattacatca ccaacgtcaa aggcggaaaa atcgaaggca aatcgcataa ccgctgggcg
     3781 attatcggct taaattaacg aaagggaaac actatgaact taaaacaatc tcttattgaa
     3841 aataactcta ttttgctcaa tcaatcagcg gcgaattggg aagacgcaat caaaatcggc
     3901 actgatttat tagtcaaagc cggtacgatt gaaccacgct attacgataa tattgtaagc
     3961 aaaatcaaag agatggggcc ttatattatt cttgcaccgg gcttggcaat gcctcacgcc
     4021 cgtccggaag aaggtgtgat taaaacctct ttcgctttag tgacttttga caccccgatt
     4081 tattttgagg gcgaagatga gccggttcac gttatgctaa cccttgcagg gagtgactct
     4141 gatcagcata tgcaaggctt ggtagaaatc acccaagtgc tagatgaccc agagagcgac
     4201 acaggtgttg atttagataa aatccgccgc tgtaaaacgg ctgaagaagt gtatgcagta
     4261 atcgatcagg cattagccta atattctaca agcggttcaa aaacatcaat attttgcaaa
     4321 tagaggaata caattatgtc caaaccatta ttacaaattg cccttgattc tctttcactg
     4381 gaaaaagcgg tggcggatgc aaaacaagca gaaaaccaag tcgaaattat tgaatgcggt
     4441 actattcttg cttttgctga gggtatgaaa gccgtttcca ccttgcgtgt attgcaccca
     4501 aatcacatta ttgtgtgcga cttaaaaacc accgatggcg gtgcaatttt agctaaaatg
     4561 gcatttgaag ccggtgccga ctggctcacg gtttcggctg cggctcaccc tgcaaccaaa
     4621 gcggcttgca aaaaagtggc ggacgaattt aacgctgctc acccggagct aaaagtgaaa
     4681 aaagagattc aaattgaaat ctacggcaac tggacggtgg aaaaagatgc caaagagtgg
     4741 gtagaattag gggtaaccca agcgatttac caccgctccc gtgatgcgga actagcaggt
     4801 aaaggttgga caacggaaga tgttgagtta atgaaacagc tttctgactt aggcattgaa
     4861 ttatctatca ccggcggtat cgtaccggaa gatattcacc tgttcaaaga gatcaaaaac
     4921 gccaaagcct ttattgcagg ccgtgcttta gtcggtgaaa aaggcaaaca aactgccgaa
     4981 gccattcgtg tggagattga taaatactgg aactaatccg gcattgaaac ccaataaaaa
     5041 tggcgttctc tcaaccggaa cgccattttt tatctcattt tttctattct tgcaaggccg
     5101 atattttcca caatctcgct acattttcgg cagttgaaat caggttctct ttgcctgatt
     5161 ttaacaccgc ttctaaattg ctgacctgtc gaataatcgg aaataccgca tcaatgccgt
     5221 gttggtacac cacagggtaa tcttctttca atgagccaac tatcgcaatc accggcttgc
     5281 caaactgctt agcgactttc gccaccccaa tcggggtttt accggcgata gattgagcgt
     5341 ccattcgccc ttcccctgtc accaccaaat ccgcatcggc aagtttctca gcaagatcgg
     5401 ttgcctcaag aataatttgc acgccggaac gtaatgttac gttaggcagt agcagtaaac
     5461 caccgcccat tccaccggct gcaccggcac cggctttctc ggcaatctca atgcctaatt
     5521 gggattttac ggtttcggca aaatgcaaca gggcgtggtc gagcatttta accatttccg
     5581 gtgtagctcc tttctgtggc ccaaaaattg ccgaagcccc tcgctcgcca cacagcggat
     5641 tatcgacatc gcaagcaacc tcaaattcca cttgctttaa gcgtgaatcc aaaccgctta
     5701 aatcaatctg ctgaatttgg cttaatgcct cgccaccaaa gccgatttcc cgctgttcgg
     5761 catttagaaa tttcgctcct aaggcttgta gcattccgac accgccatcg ttagtcgcac
     5821 tgccgccaat gccgagaatg attttttcca cccctaaatc taaggcggct ttaattaact
     5881 gccccgtgcc gtagctggtg gtaaccagcg gatttcgttg ctccattggc actaaatgca
     5941 agccacttgc cgccgccatt tcaataaagg cggttttttg atcccccgac aagccaaaaa
     6001 agccctccac ctcatttgca agcggtgcaa ttacgctgat tttttgcaaa ctgccgtgag
     6061 tggcatcaac cagcgactgt accgagcctt cgccaccgtc tgccatcggt acgagctgat
     6121 actccgcctc agggaaaaca cgggcaaaac cctgttgaat tgcttgagcc acttctaatg
     6181 cggtcaaact ctctttaaat gaatccggtg caatcacaat tttcatcgtc aatccttaga
     6241 gtaaaatcaa tgaaagcaga taaaccgtga gcatacccac cacacccata atgaaagtta
     6301 gggtagtttg cgtacgatag ccttgttgcg gagtcagttt agtaaagttt gttaccaccc
     6361 agtagtagct gtcattggcg tgggaaacgc acattgagcc agccgcaatc gccattacgg
     6421 ttaaggctgc cgccatttca ctggttagcc ctaaggcgtt cattaatgag tccggtgcgt
     6481 tgaacattcc cataatggag gcggtggtga taattgccac cgttgagctg ccttgtgcgg
     6541 tttttaaaat tgccgaaatc aggaacggga agaaaatccc tgctgtactg ataatatgag
     6601 cgttttgctt gatgtaatcc acaaagccgg cttgagtaat cacgttacct agcacaccac
     6661 cggcggcggt aataaagaga attgggccga ctaatttcag ggtatcattg gttaagctgt
     6721 caaattccgc cattttaccg cttgaaacca gtaggaaaat agcgaagatc accccaattg
     6781 ctaacgcaat aatcgggttg ccgacaaatt gtaggaatac gccgaaatcg ccacttacac
     6841 cggcaatttt ggcaatcgag ccgatagcca ttaagataat cggcataaaa atcggggcaa
     6901 agcttaaaaa cccacttggt aatttgccgt attgtttgat cagatcttca tagctttggc
     6961 tgattacttg atccgcttga gcttcttcca tcacgctgac ttgtttgcca acatatttgg
     7021 caaagaagta actggcaatc aacacaggaa tcgaaaccac aaagcccatt ccgatcacaa
     7081 gcaggagatt atggcttaaa ccaacagcac cggctgctgc aatcgggccc ggtgtcggag
     7141 gaataaagac gtgggaggca tataaaccgg cacttaacgc caccgccatt cccaccggat
     7201 tggcaagaat ttttttgcga atagcttcac gaatcgggtt taacaccaca aagccgctat
     7261 cacagaacac tgggatacca accacccagc ccataatcaa cattgccagt tcagggcgtt
     7321 tttgccccac cacttttacc accatatcgg ctaatttgag ggctgcaccg gttttttcta
     7381 aaatcgtgcc gataatcgcc ccaaaaataa tcacgatacc gatacttttg aaggtattac
     7441 taaaaccttc gcccacaata gttggaattt tggcaagcgg taaacctgcc actaatgcaa
     7501 gggctatcga aatcgacatt aaggctaaga acgggtgaac tttccacttc gcaatcatat
     7561 aaatcattat gatgatagcg accagaaaga tcagaataag tgcaatacct gacataataa
     7621 gtctcctcat tgtagaaaaa tgaacttatt attcggtatt tattgctaaa aagatttaga
     7681 taaatttgca aaaaatttgt taaaaacgac cgcttgtttg tatattttta caatttttac
     7741 gacagaaccg ctccaagata cagcacaaat ttttgctcta tgttattgaa agataaacca
     7801 gttatctgct caatcttctc taaacggtaa cgtagcgtat ttgggtgaat aaacaatttt
     7861 ttggcagcgt gatccagatc acaatttgcg aaaaaatagt aacgcagact tttcagtaac
     7921 acctgttttt tatcggaaac cagtaaagct tggaacggcg agagcaaggt tttcgcttcc
     7981 caagagtggc tgaaactgtg caagagggcc ggcagtttaa aatcttcaaa atgaataatc
     8041 tgcttacggc tttggatctg ctcggcataa ttgagggtat ggcaggcggt ttggtaggaa
     8101 atatgggttt gcgataaatt ttcgaccgcc agcccaacca ccgttttgaa ggaaatatcc
     8161 ggatcgttaa gtttgtttag tgcattgcgg attaacgatt tttcgttgga taaatgattc
     8221 agcaaggcaa ttttatctaa cccaatcacg gcggtgagta ggctcgggaa ataatgttcg
     8281 aaaaaatcaa tcaggcgttg gagtttttcc gcagtagagc cgtgaatttt aatcaccatc
     8341 accgacagcg gttttgcaaa atcttgcccg aaaaagaccg cttgttgctg gatttctgct
     8401 gtgcttaggc tacctttgag caaggcccgc acaaactctt cccgatagcg gcgttgccaa
     8461 cgttcctgtt ccaactcaaa cgcttgctgc ataatcagct ccgccgccat tttcaccaat
     8521 tgagcatatt gccgaacttg ttgtggctgc cctgaaatcc ccactacacc aatggttgag
     8581 cctaaatagt gaagcggtaa gttaatgccc gctttcactt catcgcacca ctgttttgca
     8641 agattttcat caatttcgac cgcttgatcg ttgcgtaacg caatcaccgc tcctgtgtgt
     8701 cgttgcccga tacgaccgac atcgccggaa gcaataatca ccccttcact atccatcaca
     8761 ttgacggagt tgggaataat ctgcatcgtg cgcttgacga tgcggtcggc aatcgcttgg
     8821 ttgagtttca ttatatgatt cctactcatt cccaatacta tgaaaaatcc ctctttcgag
     8881 ggatttgcaa attatctacg cttccacttc ctgagaatag atcatagcgt gaagttattt
     8941 tgccacagtc gctttaatta atttttccgc tttggcaagg ttgttttcta catcagcata
     9001 atcggtatta gcgtgagcca ccacttgatc ggcacgattt aacattactt cgaccttgcc
     9061 cttgttcggt tcggtaatta acacttttaa cggtaattgc aacgctaaca tcggatcttt
     9121 caccattaat ggcgtaccgg ctttcggtgt gccgtaaacc aatacggttg ccggttgcat
     9181 ttctaagccg gcttgttttg ccgccgcttg gtgatcgatt tcggcaaaaa tcgtcattcc
     9241 tttttctttt aaggtgttgg ttaaagttgt gactgtagtt tggaaatcaa atttactttc
     9301 aaataccacc ggtttgacat cggcaatatc tgccattgcc acgctgctca ttgctaatgc
     9361 agaaactgcg actgctttta agttcatttc ttttctcctc aaatagatta aataatccct
     9421 tgttcttcca acttgcctaa gaaataaggc atctgtttac gccaagtcgg ccagtcatgg
     9481 tcaatatcaa agccccagta gtcaaaccaa gcatcgatcc cttttgcgtt aaaagcctct
     9541 tgtaatcggc gggtgtcttc aatatgctgt tcttcccacg caccttgtcc gactgcaatc
     9601 acatagtgat tttggcggta gcggtctaag aaccaatcgt cattttgacc ccatacataa
     9661 tcaatcggcg agttaaaata aaccgcttta tcgccataaa actcaccact gaaaaagcga
     9721 gcgtcataaa caccgcttaa ggcgatggca atatcaaaca gatccggatg acgtaaggca
     9781 aagtttaccg tatggaaagc ccccatacta caaccggtgg caatcatcgt gccgttccag
     9841 tgcgattcat ggcggattaa cggcactagc tcgtgaataa tataacggtc gtaagcatta
     9901 tgagctaatg ccatatcgtg tccggattta tgtttagata accaagattc cgcatcgtaa
     9961 gaatcaggcg tgtaaaattt gattaagcca cgttcaataa aggaacggca agcctcaatc
    10021 attccaaaat taccatactc gttttcattg ccgccggagg aagggaaaac aataaccggt
    10081 ttacctgcgt gtccgtaaac gttgaacgac atttcacgac ctaattctcc actccagtgg
    10141 cttctttttt caaaatgcat acttcacctc ttactgttta gcttgtgcaa attgtgcaat
    10201 ttcacgcatt tgctccattg tttcggtacg ggctaaaatg ccttgttcgc ccatcacttt
    10261 ggcaaatacg cccggtacgc tttgcacgct gataatattg ccggcaaatt tctcacaaac
    10321 agcttcaatc gaattcacat aattttggtt tgccttgcgg gaaatataaa ccacgttcca
    10381 agggtgctga atatcggcat agaatttgtt ttctttcaca atattggcaa attcacggaa
    10441 cacatcgaaa tcgttggcgt agttccacat atcaatagtt aagccacccg gcggacggca
    10501 gttgatttcg agtggtaaca actcttcttt tttgctccct ttaacacgga agaactcaaa
    10561 atggaaaaaa cgctctttta cgttaaaggc tttcacacat tgtttgccca tttccactaa
    10621 ttttggcgaa atttcacatg ggacatagta gaacatatcg ccatcttttg aaacggtatc
    10681 taataccgct tctgaatatt caaggctgga gtagaaaacg atattgccct ctttatcggt
    10741 taaaccgtca aaggtaataa tatcgccgtc aataaactct tccataatat attcaaccgt
    10801 tggcgtttta taaccgaaga aagcttctaa ctcttccgca gttttgattt tgtaagtatc
    10861 gcttgcccca acccctgaat ttggcttgat aatcaccggg aatttaagct gttttgctaa
    10921 tttgcgggca tcttcatcat ctttaaatac tcgcccttgt gccactttca agccggtttt
    10981 gcggaatacc tctttcattt gggctttggt tttaattgag agcatatcat cgctcttata
    11041 accaaacaca ttgaaatcgg tacgcaattt cgcatctaac tccagccaat attcattgtg
    11101 cgattcaatg cggtcaattc gcccgtattt atgggcaaag tacgcgaccg cacgataaac
    11161 ttgttcgtaa tcttccatat tatctacacg ataatattca gtcagggaat gacgcagatt
    11221 ttcgctcaat gattcataag gggcatctgc aattcccaat gtattaaaac cggcttcacg
    11281 caaacgaatg gcaaaggttt caaagttcgt tgggaagtgc ggggaaatca tcacaaaatt
    11341 aagtggtcta gacatagttc ttcctttata aagtttgcat attattaaaa tgattagaat
    11401 agattttagg c
//
LOCUS       DANLZC010000083        10963 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.083, whole genome shotgun sequence.
ACCESSION   DANLZC010000083 DANLZC010000000
VERSION     DANLZC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10963
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.083"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..1285)
                     /locus_tag="PXE44_001657"
     CDS             complement(<1..1285)
                     /locus_tag="PXE44_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL5589157.1"
                     /translation="MDSIPLSSLFITLAILLVLSAFFSGSETGLMSLNRYKMRHLAEQ
                     GHKGAKLAEKLLARTDVLLSLILICNNLVNIAASAIATMIGMQLSGEAGVAIATGLLT
                     FVMLVFSEILPKTVAAIYPEKVGFFASFVLTPLKKLLSPIVFLMNLIIAGLMKLLRIQ
                     QKDKQGLSSEELRSVVLDAGKFIPAEHREMLVSILDMEKVTVDDIMVPRNDISGIDID
                     DDWKAIMRQLNHAPHARVIIYKGDMDNNIVGILRVREAFRLMLEKNEFTKEMLLRATD
                     EVYFIPEGTPLTTQLMNFKANKERIGLVVDEYGDIKGLVTLEDILEEIVGEFTTSSAP
                     TLEQEVQQQSDGTVIIEGSANLRDLNKLFDWDLPLEEARTFNGLILEHLEKIPDEGME
                     FELYNLKVMILEVSENMVKQAKVEHLSPKIEKEQDS"
     gene            complement(1409..2206)
                     /gene="ccsA"
                     /locus_tag="PXE44_001658"
     CDS             complement(1409..2206)
                     /gene="ccsA"
                     /locus_tag="PXE44_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDL5589158.1"
                     /translation="MLLPLLSIVAYALALLWVAPTLVNLENAATVNKKPNMMLVFGFG
                     LLATILHFSVLYNELFLIEGQNFTVTNVISLMSFIMAACVTLALLKWKTVWFPLIIVY
                     SFAICSVGVAGFVKGSFIRNVQENAGLLFHLGIALFSYALFFLALIYAFQLKWLDRKL
                     KSKTMFFCQILPPLMTVERHFFTLTLAAQAMLTLTLFSGVVYLHNFFEPEHIHKAIFS
                     FLAWLVYSIQLLGQWKLHWRGKRVLIYSISGMMLLTIGYFGSRLIVN"
     gene            2422..2946
                     /gene="yceD"
                     /locus_tag="PXE44_001659"
     CDS             2422..2946
                     /gene="yceD"
                     /locus_tag="PXE44_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDL5589159.1"
                     /translation="MQKVKLPLTIDPYKDAQRRVDYEGYISGKLLTRLGESVCNVQSD
                     AQVTLSLYVDPQRLTVIKGTAKVDVEFDCQRCGDPFTQTLDCSFVFSPVSNMDQADIL
                     PEIYEPIEVNEFGEVNLLDMIEDEFILELPLVPMHSEEHCEVSVSEQVFGELPEELVQ
                     KPNPFAVLANLKKN"
     gene            2963..3133
                     /gene="rpmF"
                     /locus_tag="PXE44_001660"
     CDS             2963..3133
                     /gene="rpmF"
                     /locus_tag="PXE44_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL5589160.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTTAAVSVDKTSGETHFRHHVTA
                     DGYYRGRKVINK"
     gene            3152..4174
                     /gene="plsX"
                     /locus_tag="PXE44_001661"
     CDS             3152..4174
                     /gene="plsX"
                     /locus_tag="PXE44_001661"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL5589161.1"
                     /translation="MVDITLAVDVMGGDFGPRVTIPALEKALKQNPSLSFLLFGDEHQ
                     IAPLLQKHTLLNHPKIQIHHTEQVIEADVPFTTAIRQSKNSSMRLAIEAVKEGKAQGC
                     VSGGNTGALMGLAKLLIEPLPNIERPALTSLIPTLNGKSSVMLDLGANIEVSNHQLQQ
                     FAEMGNLFAQVMLDLVYPRLSLLNIGTEENKGTAQLQAVHQLLKNRQDLNYTGFIESD
                     KLTSYLTDVIICDGYTGNIALKALEGAAKNIISLFKKEKADSNICRNTKRYLLKAMFY
                     RYYRKLQQINPDRHNGATLLGLSKVVVKSHGGANINAFYYAIDYAVQQIQGDIPNKIL
                     EGLTQK"
     gene            complement(4271..5554)
                     /gene="hisD"
                     /locus_tag="PXE44_001662"
     CDS             complement(4271..5554)
                     /gene="hisD"
                     /locus_tag="PXE44_001662"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDL5589162.1"
                     /translation="MQTLIWNNLTETEQQQYLARPAQVVGESIKAAVNEIKANVIANG
                     DKALFELAEKFDKVKLESLVVSEQQIQQAQSRISPELKQAIQNAKANIEAFHKAQQNQ
                     EVDIETQPGVCCQVITRPINAVGLYIPGGSAPLFSTVLMLAVPAKIAGCNKIVLCSPP
                     PIADEILYTAHLCGVETIYAVGGAQAIIAMANGTQTVAKVDKIFGPGNAFVTEAKRQV
                     VQEGTAIDMPAGPSEVLVIADEFADPAFVASDLLSQAEHGADSQVILVTPSESLARAV
                     DEELEKQVAVLPRAETARKALAHSRTFIAENLQQAVEISNKYAPEHLIVQTENARSLL
                     ADLDNAGSIFLGAYSPESMGDYASGTNHVLPTYGYTKTYSSLGLADFSKRMTVQELTP
                     QGFLALAETVERMASAELLDAHRNAVSLRVAKLKG"
     gene            complement(5566..6465)
                     /gene="hisG"
                     /locus_tag="PXE44_001663"
     CDS             complement(5566..6465)
                     /gene="hisG"
                     /locus_tag="PXE44_001663"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDL5589163.1"
                     /translation="MTTQNRLRIAMQKKGRLSQECSELLKQCGVKIIWNDQRLIAYSE
                     NMPIEILRVRDDDIPGLVFDGVVDLGIIGENVLEEVELGRLSAGENVAYKKLRQLDFG
                     GCRLSLAIDRDKNYNNIKDLEDAVIATSYPNLLKRYMKEQNVPFKSILLTGSVEVAPS
                     AGIANAICDLVSSGATLEANGLKEVEVIYRSTACLIQRQEPLSAEKQALVDKLLTRIQ
                     GVQQANESKYIMLHAPKDKLAEITALLPGVENPTILPLANDQTRVAMHVVSQENLFWE
                     TMEQLKEMGASSVLVLPIEKMLA"
     misc_feature    complement(6498..6625)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            6824..7681
                     /locus_tag="PXE44_001664"
     CDS             6824..7681
                     /locus_tag="PXE44_001664"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL5589164.1"
                     /translation="MQISLKKIYSGKVRDLYEIDEKRMLMVATDRLSAFDVILDDPIP
                     RKGEILTQISNFWFNKLAHIMPNHFTGDSVFDVLPQEEAEAIQHRAVVCKRLIPVKIE
                     SIVRGYLTGSGLKDYQQTGTICGLTLPEGLVEASKLPQPIFTPSSKAEVGDHDINISY
                     EECERQIGQELAAKVRDAALKLYTEAANYALTKGIIICDTKFEFGLDENGELTLMDEV
                     LTPDSSRFWSVETYREGTNPPSFDKQFVRDWLENSGWNKQPPAPKIPAEVIEKTVAKY
                     QEALDLLTK"
     gene            complement(7822..9303)
                     /gene="ilvC"
                     /locus_tag="PXE44_001665"
     CDS             complement(7822..9303)
                     /gene="ilvC"
                     /locus_tag="PXE44_001665"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDL5589165.1"
                     /translation="MANYFNTLNLRQKLDQLGRCRFMDRDEFADGCNFLKGKKIVIVG
                     CGAQGLNQGLNMRDSGLDIAYALRAEAIAEKRASFKRASENGFVVGTYEQLIPEADLV
                     INLTPDKQHSKVVADVMPLMKQGAAFGYSHGFNIVEEGEQIRPDLTVVMTAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGLAIAKAWASATGGDRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSIVCYDKLVSEGKDPAYAGKLIQYGWETITEALKQGGITLMMDR
                     LSNSAKLRAFELSEQIKEELAFLFEKHMDDIISGEFSSTMMADWANGDANLLKWREET
                     GKTAFENAPQADGIKISEQEYFDNGVLMVAMVKAGVELAFDTMVSAGIYEESAYYESL
                     HELPLIANTIARKRLYEMNVVISDTAEYGNYLFSHVATPILAEKIIPTLEKGDLGEPT
                     PAVEIDNVTLRDVNDAIRNHPIELIGQELRGYMTDMKRISSQG"
     gene            9525..10760
                     /locus_tag="PXE44_001666"
     CDS             9525..10760
                     /locus_tag="PXE44_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HDL5589166.1"
                     /translation="MKINRIILQSCSECGQQVRVPLTSNGNAVCPVCHNILRKSNTWS
                     LHRSAFLALAILILLPFALTFPLMSIDLLGIKINATVWDGIWKMATEGYPYTAFMVLV
                     CAVIMPIAFVLLILTIQLQRIIRQRPRYTLIFLTKIKEWVMLDVYLVALAVAAFKIRD
                     YADLQFDINLIAFVLVTLLNTLLFIKTEPKQAWERFYPEYHALPFDHPSNPTLCPTCE
                     YCFDETILDLKGRQRCPRCESNLSISDNVKIQRVWACLIAGTIMMIPANIFPISTTEL
                     AGQVSADSLMSGVLLFMEMGSYTVAAIVFIASIAVPVSKITIIFYLLLAIHYKWKHPI
                     HWQMKLLHYVHFVGRWSMLDLFVLSLMMSLLERGQILSFSVGEAAFYFGAAVFLTMFA
                     SANLDSRMLWKIHTVQKTS"
BASE COUNT         3261 a         2456 c         2072 g         3174 t
ORIGIN      
        1 ctgaatcttg ctctttttca attttcggtg aaagatgttc aactttcgct tgtttaacca
       61 tattttcact cacttcaaga atcatcactt tcaagttgta gagttcaaat tccattcctt
      121 catcaggaat tttttctaaa tgctctaaaa tcaagccgtt aaaggttctg gcttcctcaa
      181 gcggtaaatc ccaatcaaat agtttgttca gatcacgcag gttggctgag ccttcaataa
      241 tcaccgtacc atctgattgt tgctgaactt cttgctcaag ggtcggtgct gatgaagtcg
      301 taaattcacc cacaatttct tctaaaatat cttcaagagt gactagccct ttaatatcac
      361 catactcatc aaccactaaa ccaatacgct ctttattcgc tttaaagttc attaattgtg
      421 tagtgagtgg tgtgccttca ggaataaaat agacttcatc tgttgcacgc agtagcattt
      481 ctttggtaaa ttcgtttttc tccaacatta aacgaaaggc ttcacgcacc cgcaaaatcc
      541 caacaatatt gttgtccata tcccctttgt agataatcac ccgagcgtga ggggcgtgat
      601 taagctgacg cataatcgct ttccagtcat catcaatatc aatgccgctg atgtcgttgc
      661 gaggcaccat aatgtcatca accgtcactt tttccatatc taaaatagag accaacattt
      721 cacggtgttc ggcaggaata aatttaccgg catcaagcac tacactacgc agctcttcgg
      781 agcttaagcc ttgtttatct ttttgttgaa tccgcaataa tttcatcagc ccggcaataa
      841 tcaaattcat taaaaataca atcgggctga gtaatttttt aagcggtgtt agcacaaagc
      901 tggcgaaaaa accgactttt tcagggtaaa ttgccgctac tgttttaggt aggatttccg
      961 aaaaaaccaa cattacaaaa gttagcaaac cggttgcaat tgccacaccg gcttcaccgg
     1021 ataactgcat accgatcatc gttgcaatcg cagaggcggc aatgttgact aagttattac
     1081 aaattaagat aaggctaagt agcacatcgg tgcgtgccaa gagtttttcc gccagtttcg
     1141 cccctttatg cccttgctcg gcaaggtgac gcattttgta gcggtttaaa gacatcaagc
     1201 cggtttccga gcctgaaaaa aatgcagaga gtactaaaag aatagctaag gtaataaata
     1261 aactactcag gggaatactg tccaaaattc aattcctatg taaattaatc ataaaaggta
     1321 gcgtaacact aaaagcgttg tcactacctc actttgtcgg ttcagccgac ttgcaaaaaa
     1381 ttttgctttt ttgaccgctt gtagatcatt aattgacgat caaacgacta ccgaaatagc
     1441 cgatggtgag caacatcata cccgaaatcg aataaataag cacccgtttt ccacgccaat
     1501 gtaatttcca ctgtccaagt aactgaatgc tataaacaag ccaagctaaa aaggagaaaa
     1561 ttgctttatg aatatgctcc ggctcgaaaa agttatgcaa ataaaccaca ccggaaaata
     1621 aagtgagcgt cagcatagct tgtgcagcta aggttaaagt aaagaaatgg cgttcaaccg
     1681 tcattagcgg tggtagaatc tggcaaaaga acattgtttt gctctttagt tttctatcca
     1741 gccatttgag ttgaaaagca taaattaatg ctaaaaagaa gagagcataa gaaaataatg
     1801 caatccctaa atggaaaagc agtcccgcat tttcttgcac attacggata aaactacctt
     1861 ttacaaatcc agccaccccc acactacaaa tggcgaaaga gtacacaata attaaaggaa
     1921 accaaaccgt tttccatttt aacaacgcaa gtgtgacaca agccgccata atgaaactca
     1981 tcaaagagat tacatttgtt accgtaaaat tctgcccttc gattaaaaat aattcattat
     2041 ataacacaga aaaatgcaaa atcgtggcaa gtaacccaaa cccaaagact aacatcatat
     2101 taggcttttt attaactgtt gcggcatttt ctaaattcac tagagtaggg gcaacccaaa
     2161 gtagcgctag agcgtaagca acaatggata aaagaggaag taacatattg tctcgaatct
     2221 cgtcaaataa gaataactat cagaatttta atcaaaagta accgttttag ctagtttttg
     2281 ctgttgctag atcaaaaatt atcctaaaaa atgtgctttt cgccttaaat tgaaccctac
     2341 cttttctcgg taagattatc tcactgaaaa caataatttt cctttgacta atttgctttc
     2401 tatctgtaat atgcacaccc tatgcaaaag gtaaaactac cccttacgat tgacccttac
     2461 aaagatgctc agcgtcgcgt tgattatgaa ggctacattt caggtaaact tctaacacgc
     2521 ttgggtgaat cagtatgtaa tgtgcaaagt gatgcacaag tcactctctc gttatatgtc
     2581 gatccacaac gcctaactgt gattaaaggc acagcaaaag tagatgtgga atttgattgc
     2641 caacgatgtg gtgacccgtt tacacaaacg ctcgactgtt catttgtttt cagtccggtg
     2701 tctaacatgg atcaggcgga catcttgccc gaaatttatg aacctatcga agtaaacgag
     2761 ttcggtgaag taaatttact agatatgatt gaagatgaat ttattcttga attacctcta
     2821 gtcccgatgc atagtgaaga acactgtgaa gtgtccgtaa gtgaacaggt atttggcgag
     2881 ttgccggaag aactggtaca aaaaccaaac ccgttcgcag tattagctaa tttaaagaaa
     2941 aactagatct aggagaaagc ccgtggctgt tcaacaaaat aagaaatctc gttcacgtcg
     3001 tgatatgcgt cgttcacacg atgcattaac aactgcagca gtttcagtag ataaaaccag
     3061 tggtgaaact cactttcgcc accacgtaac tgcagacggt tactaccgtg gtcgtaaagt
     3121 aatcaacaag taattccttt cgaggtttat cttggttgat atcactcttg cagtagatgt
     3181 gatgggcggg gactttggtc cccgcgttac tattcctgca ctggaaaaag ccttaaaaca
     3241 aaatccaagc ctttcttttt tattatttgg tgatgaacac caaatagccc ctttattaca
     3301 aaaacacaca ctactcaacc accccaaaat ccaaattcat cataccgaac aagtgattga
     3361 agctgatgtt ccgttcacta ctgcaatccg ccaaagcaaa aacagctcta tgcggttagc
     3421 gattgaggca gtaaaagagg gcaaagcaca aggttgtgtt agtggcggta acactggtgc
     3481 gttaatggga ttggcaaaat tattgattga accattacca aatattgaac gccctgcact
     3541 cacttcgcta attcctaccc tcaacggcaa atccagcgta atgttagatt taggggcaaa
     3601 tattgaagta agcaatcacc agctacaaca atttgcagaa atgggaaacc tgtttgctca
     3661 agtaatgctt gatttagttt accctcgcct gtctttattg aatattggta cagaagaaaa
     3721 taaaggcacg gcacaacttc aggcagtgca tcaactgctg aaaaacagac aagacttaaa
     3781 ttacaccggc tttattgaaa gcgataaatt aaccagctat ctcactgatg tgattatttg
     3841 tgacggctac accggcaata tcgcgttaaa agcgttggaa ggggcagcta aaaatattat
     3901 ttctctcttt aaaaaagaga aagccgattc caatatttgc cgtaacacca aacgatattt
     3961 actgaaagcg atgttctacc gttactaccg caaattgcaa caaattaacc ctgaccgcca
     4021 taacggtgcg accttgctcg gcttatctaa agtagtcgtg aaaagccacg gcggggcaaa
     4081 tatcaatgcc ttttattacg ctattgatta tgcggtgcaa caaattcaag gcgacattcc
     4141 aaataaaatc ctagagggtt taacccaaaa ataagcgaac tcaactgttc gcttattttc
     4201 atttcttgtt tcacttaaag atacaagcgg tcaaatttgc aaaaaaattt acccattcaa
     4261 ccgcttgtgt ttagcctttt aattttgcca ctcgtaaact aaccgcattg cggtgtgcat
     4321 ctagcaattc tgcacttgcc attcgttcca ccgtttcggc caaggctaga aagccttgtg
     4381 gtgtcagctc ctgcaccgtc attcgcttgc taaaatcagc taaacctaag ctcgaatagg
     4441 ttttagtgta gccgtaagtc ggtaaaacgt ggttagtgcc gctagcataa tcccccatac
     4501 tttccggcga atacgcccct aagaaaatcg agccggcatt atctaaatcc gccaacaacg
     4561 agcgagcatt ttcagtttgc acaattaaat gctcgggtgc atatttatta ctaatttcga
     4621 ccgcttgttg caggttttcc gcaataaaag tgcgactgtg agccaatgct ttacgggcag
     4681 tttcggcacg aggtaaaacc gccacctgct tttctaattc ttcatcgact gcccttgcca
     4741 agctctcact tggtgtgact aaaatcactt gggaatcggc tccatgttct gcttgagaga
     4801 gtaaatcgct cgccacaaat gcagggtcag caaactcatc ggcaatcacc aacacttccg
     4861 acgggcctgc cggcatatca atcgccgtgc cttcttgcac aacttggcgt ttcgcctccg
     4921 tcacaaacgc attgcccggg ccgaaaattt tatccacttt cgctaccgtt tgtgtgccat
     4981 ttgccatcgc aataatcgct tgagcccccc caactgcgta aatcgtttcc accccacaca
     5041 aatgggcggt gtagagaatt tcatcggcaa tcggcggtgg tgagcaaagc acaatcttgt
     5101 tacaaccggc aatttttgcc ggcactgcaa gcattaacac ggtagagaaa agcggggctg
     5161 aaccgcccgg aatataaagc cccaccgcat taatcgggcg agtgatgact tggcaacaca
     5221 cccccggctg ggtttcaata tccacctctt gattttgctg tgccttgtgg aaagcctcga
     5281 tattcgcttt cgcattttga atcgcttgct taagctctgg tgaaatacgg ctctgtgctt
     5341 gctgaatctg ctgttctgat acaactaagc tctctaattt tactttatcg aacttttcag
     5401 ccaattcaaa taatgcctta tcgccgttgg caattacatt cgctttaatt tcattaactg
     5461 ccgctttaat actctcgcca accacctgtg ccggacgggc aagatattgc tgctgctcag
     5521 tttctgttaa attgttccaa attagggttt gcatatttat tccttttacg ccaacatttt
     5581 ctcaatcggt aaaaccaata cggaacttgc gcccatttct tttagctgct ccattgtttc
     5641 ccagaacagg ttttcttggc tgacaacgtg cattgccaca cgggtttggt cgttagctaa
     5701 cggtaaaata gtcgggtttt ccacaccggg taataaagcg gtaatttccg ccagtttgtc
     5761 tttgggggcg tgcagcataa tatacttaga ttcgttcgcc tgttgcacac cttgaatgcg
     5821 ggttaataat ttatccacta acgcttgttt ttccgctgaa agtggttctt ggcgttgaat
     5881 taggcaagca gttgaacggt aaatcacttc aacctctttt aatccgttcg cctctaatgt
     5941 tgcacctgaa gaaaccaagt cacaaatcgc attcgcaatt ccggcacttg gagcaacttc
     6001 aaccgaaccg gtgagtaaaa tacttttaaa cggcacattt tgctctttca tataccgctt
     6061 taataaattg gggtaagaag tggcaatcac agcatcttct aaatctttga tattgttata
     6121 atttttatca cgatcaatag ctaacgataa acggcagcca ccaaaatcta actgacgaag
     6181 ttttttatag gcaacattct caccagcact taatcgccct aattccactt cctctaatac
     6241 attttcaccg ataatcccaa gatcaactac gccgtcaaaa actaagcctg gaatgtcgtc
     6301 atcacgcacc cgtaaaattt caatcggcat attttctgaa taggcaatca ggcgttgatc
     6361 gttccaaatg attttcacgc cacattgttt aagcaactca ctacattctt ggcttaagcg
     6421 accttttttc tgcatcgcaa ttcttaatcg attttgtgtt gtcattgttg tttccttctt
     6481 atgttttaaa ttgtaaaaat aaaaaaacct ctcgaaatca cttccgagag ggttaaattt
     6541 atgctgcgtt taggcgtact cggaagtctt atcttccgca cataccaaat gcccgaaaga
     6601 ttattcaggt aaatgatggt ggtgatgagt acgaataaaa gccattttgt tgttctccaa
     6661 actaaattgc tttaaaagat acgcttaaat tttggcaatg acaagtgttt tttgcgaaaa
     6721 aattgctatt tttcatctct ctaaaacaac tatcgcaaac gtttgcgtaa agcggtataa
     6781 tttgcctgat tttttgcaaa ttcaacaaag taggaataac actatgcaaa tcagcctcaa
     6841 aaagatctac tccggcaaag tccgtgatct ctacgaaatc gatgaaaaac gtatgcttat
     6901 ggttgccacc gaccgattat ctgcctttga tgttatttta gacgatccta ttcctcgtaa
     6961 aggcgaaatc ctcacccaaa tctccaactt ctggttcaac aaattagccc acattatgcc
     7021 aaaccacttc accggcgaca gtgtgtttga cgtgttaccg caagaagagg cagaagctat
     7081 tcaacatcgt gcggtggttt gcaaacgctt aataccggtg aaaattgaat cgattgtgcg
     7141 gggttattta accggctccg ggctgaaaga ttaccaacaa accggcacga tctgcggctt
     7201 aacattgccg gaaggcttgg ttgaggccag caaattaccg caaccgatct tcacaccgtc
     7261 cagcaaagcc gaggtgggcg atcacgatat taatatcagc tacgaagagt gtgaacgcca
     7321 gatcggtcaa gaacttgccg ccaaagtgcg tgatgccgct ctaaaactct acactgaagc
     7381 ggcaaactat gccctgacca aaggaattat tatttgcgac accaaattcg aattcggctt
     7441 agacgaaaac ggtgaactaa cgctaatgga cgaagtccta accccggatt ccagccgttt
     7501 ttggtcagtt gaaacctacc gagaaggcac aaatccacct tcatttgata aacaattcgt
     7561 gcgtgactgg ctggaaaata gcggttggaa caaacaaccc ccagccccga aaataccggc
     7621 agaggtaatt gaaaaaaccg tagcgaaata tcaagaagca ttggatttat taaccaaata
     7681 acaccacaag cggtcaaatt tacgctattt tttgcaaaac aaaaacctgt aagccaacac
     7741 ttacaggttt tttagttagt ggtaaaaagt gcggttattc taccgcactt ttgcaaaatt
     7801 ttgtgtaaat ctgaccgctt gttagccttg cgaagaaata cgtttcatat ctgtcatata
     7861 accacgtaat tcttgaccga ttaactcgat tgggtggtta cggattgcgt cattcacatc
     7921 acgtaaggta acgttgtcga tttccactgc cggagttggt tcacctaaat cgcctttctc
     7981 taacgttggg ataattttct ccgctaaaat cggggttgca acgtgagaga ataagtagtt
     8041 accgtattct gcggtatctg aaattaccac gttcatttca tataaacgct tacgagcgat
     8101 tgtatttgcg attaacggta actcgtgtag agactcgtag taagccgatt cttcataaat
     8161 accggctgaa accattgtat caaaggctaa ttcaacccct gctttcacca ttgcaaccat
     8221 taacacaccg ttatcaaagt attcttgctc agaaatttta ataccgtctg cttgtggtgc
     8281 gttttcaaag gctgttttac ctgtttcttc acgccatttt aataagttgg catcgccgtt
     8341 tgcccagtct gccatcatcg ttgatgagaa ctcaccactg atgatgtcgt ccatatgttt
     8401 ttcaaataag aacgctaatt cttctttgat ttgctcagaa agctcgaacg cacgcaattt
     8461 ggcactgttt gataagcgat ccatcattaa ggtgataccg ccttgtttta acgcttcggt
     8521 aatggtttcc caaccgtatt ggatcaattt acctgcgtaa gccggatctt taccttccga
     8581 cactaatttg tcgtagcaca cgattgaacc tgcttgtaac ataccgcaaa gaatggtttg
     8641 ttcacccatt aaatcagatt tcacttctgc tacgaatgaa gattctaata cgcccgcacg
     8701 gtcaccaccg gtagcacttg cccacgcttt cgcaatggct aaaccttcgc ctttagggtc
     8761 attttccggg tgaacggcaa ttaaggttgg cacaccgaaa ccacgtttgt actcttcacg
     8821 cacttccgtg cccgggcatt tcggagcggt catcacaacc gttaaatccg gacgaatttg
     8881 ctcaccttct tccacaatgt tgaaaccgtg tgaataacca aatgccgcac cttgtttcat
     8941 taacggcatt acatcggcaa ctactttaga gtgctgtttg tccggcgtta agttaatgac
     9001 taaatccgct tcaggaatta attgctcgta agtacccacc acaaagccgt tttcagaagc
     9061 acgtttaaat gaagcacgtt tttcagcaat cgcttcggca cgtaaagcgt aagcaatatc
     9121 taagcctgaa tcacgcatat ttaaaccttg gtttaaacct tgagcaccac aacccacaat
     9181 cacgattttt ttgcctttta agaaattgca gccatctgca aattcatcac gatccataaa
     9241 gcggcaacga cctaactggt ctaatttttg acgtaaattt aaagtgttga aatagttagc
     9301 cattttttaa tccttatttg aattaatgag attgatatga tgttttgttt gcacattgtt
     9361 atagtttgat ttgattatac cttaacctaa cattgcgtaa attgaaaata ttaatatttc
     9421 atattgcata aaatgcaact tgcaaaaatt ttgccaaatt taaccacttg caatctctac
     9481 gataaagtag aaaataacca cctaatttaa tagaagatca aaaaatgaaa ataaacagaa
     9541 tcattttaca aagctgtagc gaatgtgggc aacaagtgcg agtcccgctc acgtcaaatg
     9601 gcaatgccgt ttgcccggtc tgccataata tattacgcaa aagcaatact tggagcttgc
     9661 accgtagtgc ttttttggca ttagccattt taattttgct tcccttcgca cttacttttc
     9721 ccttgatgag cattgactta ctcggcatca aaattaatgc taccgtatgg gacggtattt
     9781 ggaagatggc aacggagggc tacccttaca ccgcttttat ggtgctagtc tgcgcggtaa
     9841 taatgccgat tgcatttgta ttgctaattt taaccattca gctacaacgt attattcgcc
     9901 aacgcccccg ttacacgctg attttcttaa ctaaaataaa agaatgggta atgcttgatg
     9961 tctatttagt tgctcttgcg gttgccgctt ttaaaattcg agattatgcc gatctacaat
    10021 tcgatatcaa cttaatcgcc tttgtgttgg taacactctt aaacacccta ctttttatta
    10081 aaaccgagcc aaaacaagca tgggaacgtt tttacccaga atatcacgcc ttgccgtttg
    10141 atcacccaag caatccaaca ctttgcccga cctgtgaata ttgctttgat gaaactattt
    10201 tagacttaaa aggacggcaa cgctgccctc gttgtgaaag caatttatct atttccgaca
    10261 acgtaaaaat acaacgggtt tgggcttgct taattgcagg gactattatg atgattccgg
    10321 caaatatttt cccgatttcg acaactgaac tggcaggtca ggtttcggca gattcattaa
    10381 tgtccggcgt gcttctgttt atggaaatgg gtagctacac cgtggcagcg attgtgttta
    10441 tcgctagtat tgcggtgccg gtgagcaaaa ttaccattat tttttatctc ttgctggcaa
    10501 ttcattacaa gtggaaacac ccgattcatt ggcaaatgaa actactacac tacgtccatt
    10561 ttgtcggacg ctggtcaatg ctggatttat tcgtgttatc attgatgatg tcgctactcg
    10621 aacgagggca gattctcagt ttctcggtag gggaagccgc tttctatttt ggagcagccg
    10681 tattcttaac gatgtttgcc tctgcaaatt tagattccag aatgttgtgg aagattcata
    10741 ctgtccagaa aacatcctaa ataaagattt tccgataaat aatacataac atgcaaagta
    10801 agaaaagtag tacgtaaccc aaaatctcaa tccctattga gtaagaaaga gccgataatg
    10861 tagcaccgat agccagaaag aaattagcaa atataaaata gtctttacta tatttacttg
    10921 aggcaattag ggtatccatt gcagtactaa agaacatttc caa
//
LOCUS       DANLZC010000084        10933 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.084, whole genome shotgun sequence.
ACCESSION   DANLZC010000084 DANLZC010000000
VERSION     DANLZC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10933
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.084"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            100..846
                     /locus_tag="PXE44_001667"
     CDS             100..846
                     /locus_tag="PXE44_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5589167.1"
                     /translation="MQLRHKQIMEYIQQFDEANVQELAEHCGVSIETIRRDLNKLDKD
                     GLLHRTHGGAVSHKKRDIGRSFTTRQRLNSEAKRSIAENVLEYLQPESVIALDASSTS
                     WNVAQRIPNIPCTVVSSSMRIIYSLSHKPHIKTIATGGIYSEKYDAFYGPLSEQLLTR
                     LKIDLAILSCTGIADGAIWESNEINISLKRKLIANSKQVFLLADHSKFERKDLFRIED
                     LSCIDKLFINQPPPDFLKNYCLAHQIEIII"
     gene            1042..2805
                     /gene="fucI"
                     /locus_tag="PXE44_001668"
     CDS             1042..2805
                     /gene="fucI"
                     /locus_tag="PXE44_001668"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDL5589168.1"
                     /translation="MTTLKSTPVKIGIRPTIDGRRLGVRESLEDQTMNMAKAVAQLIQ
                     SEVRHPNGDFVECVIADSTIGGVAEAAACAEKFKRENVGAVITVTPCWCYGSETIDMD
                     PHMPKAIWGFNGTERPGAVYLAAALAGHSQLGLPAFSIYGTEVQEADDQSIPDDVREK
                     LLRFARAGLAVATLRGKSYLSIGSVSMGIAGSIVNQQFFQEYLGMRNEYVDMTEIKRR
                     LDRHIYDAEEFKLAMSWVKTYCKEGIDVNAPENQRSPEEREKLWEDVVKMTIIARDLM
                     VGNPRLAELGYSEEALGHNAIAAGFQGQRQWTDHLPNGDFMEATLNSTYDWNGVRAPY
                     IMATENDSLNGVNMLFGNLLTGQAQIFADVRTYWSEDSVERVTGWRPESGFIHLINSG
                     SAALDGTGQHTDRAGKPTIKPAWEVTEEDGKRCLENTCWCPAVHEYFRGGLSSQFLTK
                     GGMPFTMHRINLIKGIGPVLQIAEGWSIDLPENVHDILNKRTNETWPTTWFVPRLTGK
                     GAFTDVYSVMANWGANHCVATFGHVGADLITLASMLRIPVCVHNVEDKDVFRPSAWNG
                     FGQDKEGQDYRACANFGPLYK"
     gene            2999..4432
                     /gene="fucK"
                     /locus_tag="PXE44_001669"
     CDS             2999..4432
                     /gene="fucK"
                     /locus_tag="PXE44_001669"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDL5589169.1"
                     /translation="MPISLIFDCGATNLRTIAINQAGEIVAAHHLPNNTQPDVESPEF
                     HIWDIEEIWSKLMTCAKQTLSQLSEQQRQEIVGISVTTFGVDGALFDKNGKQLYPIIS
                     WKCPRTLPIMENLANYLDVEALYQRNGVGHYSFNTLFKLLWLKENKPEIYQQAESFLF
                     ISSILTYLLTGVQTTDRTMAGTSMMTNIENDSWDKTVLDLLGLTENHFPPMKSAGEVV
                     GTLKGEISQELGLSGQIPVISCGHDTQFAIFGSGAGYNQPVLSSGTWEILMVRTPKAK
                     PQWQFVQNGLTIEFDSQSGYFNPGVQWVASGVMEWFGKRFFSDIANTEHYYRTMIEEA
                     AEVPAGSNGVKLVGNFDGTTAKTGSIIGLSMHITRGEIYRAGLEYMAHRLKEGLDVLQ
                     EVGHFNAESLICVGGGSKNALWNQIRADVLNRPIDVVDFPESTVLGAAMFTFTGAGVF
                     ENPNAAQQAMKPNVKRVEPSSNSQFYK"
     gene            4507..4938
                     /gene="fucU"
                     /locus_tag="PXE44_001670"
     CDS             4507..4938
                     /gene="fucU"
                     /locus_tag="PXE44_001670"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDL5589170.1"
                     /translation="MLKGIHPAISPDLLKVLAEMGHGDELVLSDAHFPAHSIHQNVIR
                     ADGLSVATLLEGISALFEFDQYVEAPLAMMQAVKGDTLDPSVEERYLNAIKKVNGTAP
                     KIERVERFAFYDRAKNAYAVVITGELAKYGNIIIKKGVTPV"
     gene            4998..5651
                     /locus_tag="PXE44_001671"
     CDS             4998..5651
                     /locus_tag="PXE44_001671"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDL5589171.1"
                     /translation="MDRKILARQIIDTCLEMTRIGLNQGTAGNVSVRYKDGMLITPTG
                     TPYEQMTEDSIVFVDGNGKHEEGKLPSSEWQFHLAVYEARPELNAVVHNHAKNCAAVS
                     ILGEPIPAIHYMIACTGTNHIPCVPYAPFGTHQLADYVREGIKHSKAILLAHHGLITA
                     DKTLEKALGIAHEVEVIAEWYLKLLATGKPIPTLNKEQMDIVLGKFKSYGSWVESEQ"
     gene            5713..7242
                     /locus_tag="PXE44_001672"
     CDS             5713..7242
                     /locus_tag="PXE44_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5589172.1"
                     /translation="MQNEVILTMKNISKSFAGVNALKGVELTLRKGEVHALMGENGAG
                     KSTLMKCLLGVYQADEGEIIYKGQPVRFESVLEAQKAGISMIFQELNLIPHLTVAENI
                     FFAREPLKHGLVDKDKMVEESAKLLNFFEIDVDPNDEVRMLSVAKQQMVEIAKALSFD
                     VEVLIMDEPTSALTEKEIDKLFELVDRLRAKGVSIVYISHRMEELKRICNHITIFRDG
                     TYVSNHKFNEITMDEIITKMVGRSLDNHFPPKTAVVTNDIILSIMNAERKGVFEPLNF
                     DLRKGEILGITGLVGAKRTELARAIFGADPLDSGEIFVHENKVSIKDPSDAIKAGIAY
                     LSEDRKLNGVAVRMSIRENITMASMDKVANQIGVISYDEEEKASKTFIDKMEIKTPTI
                     EQKVQNLSGGNQQKVVIGKWLFREAKVMIFDEPTRGIDVGAKYAIYQLLDELAANGVG
                     VIVISSELPEVLGVSDRIIVMREGRMTGMLETKKTNQEEIMHYATGVKNMFAREYGVE
                     K"
     gene            7239..8237
                     /locus_tag="PXE44_001673"
     CDS             7239..8237
                     /locus_tag="PXE44_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5589173.1"
                     /translation="MNEKQKEMLRKMAALAGLVLLIIFFSVTNEYFFTSNNIMTVGLQ
                     TSTIALIGIGATCVILTGGIDLSTGSVVALSGVAAAMIVNAGVPVPLGMILGILVGGI
                     CGLINGILVTRMKLPPFIATLGMMMVARGLALYVTNAAPVSGMPESFANLGNGALFKI
                     VEEGANGLPKVVFAGIPYPVIIMIFITVLFTFALSKLKVGRYLYAIGSNEEAARLSGI
                     KTNIVKIYAYVASGLLSGLTGVILASRLVTAQPNGGVSYELDAIASAVVGGTSLMGGV
                     GTIPGTLIGSFIIGVLRNGLNMNGVSSFVQMIVIGLVIIVAVSLDQLRQSKKAGKK"
     gene            8261..9208
                     /locus_tag="PXE44_001674"
     CDS             8261..9208
                     /locus_tag="PXE44_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5589174.1"
                     /translation="MKKSALFTATLFGLSTLFAGSAMAKSDEIAVIVKSANSTFWQNV
                     RKGAETAGKDIGGQYKVTFQGPEAETAIDAQVNMVDNAVNRGVAGIVLAASDPVALVP
                     AVKKAYESGIPVVLIDSGINSDGKYYQSFLATDNRAAGKLAAEKLLAKVKGGKVAVMS
                     FTQGAASAIERTGGFIDEIKAKADYKLVGPYYSDSDMTKALNQTEDVLGSNPDLAAIF
                     GANEPTAVGMARAVKQKGFAGKIVAVGFDGNSDLQNFVRDGTLDGIVVQSSYQMGYKG
                     VDTIGKLIKGEKVEKVIDTGVVYVTKENIDSEEAKGVLY"
     gene            9264..9515
                     /locus_tag="PXE44_001675"
     CDS             9264..9515
                     /locus_tag="PXE44_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL5589175.1"
                     /translation="MSNTLTDKALVVIAALGGRENIVDVYACITRLRVEVKNTDIVEW
                     DKIKSVGALGVVTSGSVIQSIFGSDAEAYRDEIKRILNI"
     gene            9644..10795
                     /gene="fucO"
                     /locus_tag="PXE44_001676"
     CDS             9644..10795
                     /gene="fucO"
                     /locus_tag="PXE44_001676"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL5589176.1"
                     /translation="MANRLILNETSYHGKGAIQNIVHEVTSRSFKKALVVTDKDLIKF
                     NVAQKVTALLDAAGLPYEIFDEVKANPAVDVIKAGVAKFKASSADYLIAIGGGSPIDS
                     AKAIGIIINNPEFSDVLSLEGVAPTHQKCVPIIAIPTTAGTAAEVTINYVITDEAKKR
                     KFVCVDVHDVPAVALVDPDMMESMPKSLAAATGMDALTHAIEGYITKAAWELTDALHL
                     KAIELIGRSLRGAVEKNPKDVEAMALGQYVAGMGFSNVGLGAVHSMAHPLSAYYDTPH
                     GIANAVLLPYVMEFNKNYTGEKYREIARAMGVKGVDEMSQEEYRNAAIAAVQQLSQDV
                     GIPPKLHQIGVKEADLAALSVDAFNDVCTGGNPRDCTAEELLEVYRIAY"
BASE COUNT         3278 a         2278 c         2487 g         2890 t
ORIGIN      
        1 acacaaaatg tgatcgcctt ctaaaaatca aatttgcttg ctcgacagta acgctacaag
       61 cggttaaatt tggagaaaaa tttacaaaaa ggatagatta tgcagctgcg acacaaacag
      121 ataatggaat atatacaaca gtttgatgaa gctaacgttc aagaattggc ggaacattgt
      181 ggggtttcga ttgaaactat ccgccgagat ttaaataagc tagataaaga tggattatta
      241 catcgcactc acggtggagc tgtgagccat aaaaagcgag atattggtcg ctcatttacc
      301 acccgacaac ggctgaacag tgaggcgaaa cgcagtatcg cagaaaatgt gttggaatat
      361 ttacaaccag aatcggtgat tgctttagat gccagctcaa cctcttggaa tgtggcacag
      421 cgtattccaa atattccttg caccgttgtc agcagttcaa tgcggattat ctacagcctt
      481 tcacacaaac cgcacattaa aacgattgcc accggtggta tttattccga aaaatacgat
      541 gccttctatg gtccattatc cgagcagtta ctgacccgtt taaaaattga tcttgccatt
      601 ctttcttgta caggcattgc cgatggggcg atttgggaat caaatgaaat taatatttcc
      661 cttaaacgta aattaattgc gaacagtaaa caggtttttt tattagccga tcacagtaaa
      721 tttgagcgta aagacttgtt tcgtatcgaa gatttatctt gcattgataa attatttatc
      781 aaccaacccc caccggactt tctcaaaaat tattgcttag cacatcaaat tgaaatcatt
      841 atttagtgat ttctgcccga ttttattcag aaataaaaca aatcgggcat tcttttttca
      901 ctcattttta ccaaaatccc ccattttttg tgatcgcact cacaaaattt aaactaaatt
      961 cgcatttttc ttcataactc atattgcgta tgcataataa attcaatcgc aaattttttt
     1021 aaccaatcgg gagaactatt tatgacaacg ttgaaatcta cccctgtaaa aattggtatc
     1081 cgcccaacca ttgacggtcg ccgtttagga gtgcgtgaat cacttgaaga tcaaacaatg
     1141 aatatggcaa aagccgttgc acaattaatt caatctgaag tccgccaccc aaatggtgat
     1201 ttcgttgaat gtgtgattgc tgattccacc atcggtggtg ttgccgaagc tgcggcttgt
     1261 gcagaaaaat tcaaacgtga aaatgtcgga gcggtgatta ccgtaacccc ttgctggtgc
     1321 tacggttctg aaaccattga tatggaccca catatgccga aagccatttg gggctttaac
     1381 ggaacagaac gcccaggtgc agtttattta gctgctgctt tagcaggtca tagccaatta
     1441 ggtttaccgg cattctccat ttatggcaca gaagtacaag aagctgacga tcaatctatt
     1501 ccagacgatg tgcgtgaaaa attactccgc tttgcccgtg ccggcttagc agtggcaacc
     1561 ttacgaggaa aatcatattt atctatcggc tctgtttcaa tgggtattgc cggttctatc
     1621 gtaaaccaac aattcttcca agaatatttg ggtatgcgta acgaatatgt ggatatgacc
     1681 gaaattaaac gccgtttaga tcgccatatt tacgatgccg aagaatttaa attagcaatg
     1741 agctgggtga aaacctattg caaagaaggt attgatgtca acgcaccgga aaatcaacgc
     1801 agcccggaag agcgtgaaaa actctgggaa gacgtggtga aaatgaccat tattgcccgt
     1861 gatttaatgg tgggtaatcc tcgcttagca gagctaggct atagtgaaga agccttaggg
     1921 cataatgcga ttgctgccgg tttccaaggg caacgccaat ggacagacca tttgccaaac
     1981 ggcgatttta tggaagctac cttgaattcg acctatgact ggaacggcgt tcgcgcacca
     2041 tacattatgg caactgaaaa cgacagctta aatggcgtga atatgttgtt cggtaactta
     2101 ttaaccggtc aggcacaaat ctttgccgat gtgcggacat actggagcga agactcggta
     2161 gaacgtgtca ccggctggcg tccggaaagc ggttttatcc acttaatcaa ctccggctct
     2221 gcggcgttag acggaacagg tcagcatacc gatagagcag gcaaaccaac tattaaaccg
     2281 gcttgggaag tgactgaaga agacggtaaa cgttgtttag aaaacacctg ttggtgtccg
     2341 gcagttcacg aatatttccg tggtggttta tcttcccaat tcttaaccaa aggtggaatg
     2401 ccgtttacga tgcaccgtat caacctgatc aaaggtatcg ggccggtttt acaaattgcc
     2461 gaaggttggt ctatcgactt gccggaaaat gtacacgaca ttttaaacaa acgcaccaat
     2521 gaaacctggc cgaccacttg gttcgtacct cgcttaaccg gaaaaggcgc atttaccgat
     2581 gtgtatagcg taatggcgaa ctggggtgca aaccactgtg tggcaacctt cggacacgtt
     2641 ggggcagact taattaccct cgcctcaatg ctccgcattc cggtttgtgt gcacaatgtg
     2701 gaagataaag acgtattccg cccaagtgcg tggaacggtt tcggtcaaga caaagaaggt
     2761 caagactacc gtgcttgtgc taacttcgga ccattatata aataatacac aatcaccctc
     2821 tccccttgtg ggagagggac agccaagcaa ttcgttcaga attgaatggc agggagaggg
     2881 ctacaagcgg tcgcttttct ctcaaaattt acaaatccct ctctctgatt aaattgctaa
     2941 acgcaattta atctatctct ctaaaaggga gatattagga taagtaaagg agataacaat
     3001 gccaatttct cttattttcg actgtggtgc caccaacctc cgtacgattg cgattaatca
     3061 agcgggtgaa attgtggcag cccatcatct accgaataac acacaaccag atgtggaaag
     3121 cccagaattt catatttggg atattgaaga gatctggtcg aaattaatga cctgtgccaa
     3181 acagacgctt tctcaacttt cagaacaaca acggcaagag attgttggga tttcagtaac
     3241 cacctttggc gtggacggtg cattatttga caaaaacggc aagcagcttt atccgatcat
     3301 ttcttggaaa tgcccgagaa ccctaccgat tatggaaaac cttgccaatt atcttgatgt
     3361 tgaggcgtta tatcaacgta atggcgttgg acattatagt ttcaacacct tattcaaatt
     3421 attgtggtta aaagagaaca aaccagaaat ttatcaacaa gctgagagtt tcttattcat
     3481 ctcatctatt ttgacttacc ttcttacagg cgtgcaaaca accgaccgca caatggcggg
     3541 aacgtcaatg atgacaaaca tcgaaaacga ttcgtgggat aaaacagttt tagatttatt
     3601 aggcttaacc gaaaaccatt ttcctccaat gaaaagtgca ggagaagttg tgggaacatt
     3661 aaaaggagaa atttcccaag agttaggttt aagcggtcaa attccagtaa tttcttgcgg
     3721 acacgatact caatttgcca tttttggctc aggagctggc tataaccaac ctgtgttaag
     3781 ttcaggcact tgggaaattt taatggttcg taccccaaaa gcaaaaccac aatggcaatt
     3841 cgttcaaaac ggcttaacca ttgagtttga tagccaatct ggttatttca acccaggtgt
     3901 gcagtgggta gcttcaggtg tgatggagtg gtttggcaaa cgtttcttct ctgatatagc
     3961 caatactgaa cattactacc gcacaatgat tgaagaagcg gcagaagtgc ctgctggctc
     4021 aaacggtgtg aaattagtcg gcaattttga tggcacaaca gctaaaacag gttcaattat
     4081 cggcttatct atgcacatca ctcgtggcga aatttaccgt gcaggcttgg aatatatggc
     4141 tcatcgtcta aaagaagggt tagatgtatt acaagaagtc ggtcatttta atgctgagag
     4201 tttgatttgt gtgggaggtg gttctaaaaa cgctttatgg aatcaaattc gagccgatgt
     4261 cttaaataga ccgattgatg tagtggattt ccctgaaagt accgtattag gcgcggcaat
     4321 gtttactttt acaggtgctg gtgtatttga aaatccaaat gcggctcaac aggcaatgaa
     4381 gccaaatgtg aaacgggttg agccatcgtc aaatagtcaa ttttataaat aaagtttctc
     4441 ccctctctct gatttgaggt tcattcagaa catcaaatca gagagagggt aaaaaaggag
     4501 ttcaaaatgt taaaaggtat tcacccggca atttctccag atttattaaa agtcttagcc
     4561 gaaatggggc acggcgatga attagtgctt tctgatgcac acttccctgc tcactctatt
     4621 catcaaaacg tcattcgtgc agatggctta agtgttgcca ccttattaga aggcatttct
     4681 gctctgtttg aatttgacca atacgttgaa gccccacttg caatgatgca agcagtaaaa
     4741 ggcgacacgc tcgatccaag cgttgaagag cgttacctca atgcaattaa aaaagtaaac
     4801 ggaacagccc cgaaaataga gcgtgtagaa cgcttcgctt tctatgatag agccaaaaac
     4861 gcttatgccg ttgtcattac aggcgagtta gccaaatacg gcaacattat tatcaaaaaa
     4921 ggcgtaacac cggtttaatt acaagcggtt aaaaaagcag aattttttac aaatataaaa
     4981 ttaaaaggag ttcaacaatg gacagaaaaa tactggcgag acaaatcatt gatacttgtc
     5041 ttgaaatgac ccgaattggg ttaaatcaag gtacagcagg caatgtgagc gtacgttata
     5101 aagacggtat gctcattacg ccaacaggta caccttatga gcaaatgacc gaagattcta
     5161 tcgtctttgt tgatggcaac ggcaaacacg aagagggaaa actcccttca agtgagtggc
     5221 agttccattt agcggtttat gaggctcgcc ctgaattaaa tgcggtagtt cacaaccacg
     5281 ctaaaaactg tgccgcagtg tctattttag gtgaacctat ccctgccatt cactatatga
     5341 tcgcctgcac cggcactaat cacattcctt gtgtacctta tgccccgttc ggcactcacc
     5401 aattagccga ctatgtacgt gaaggcatta aacacagcaa agcaatttta ctcgctcacc
     5461 acggcttaat caccgcagat aaaaccctcg aaaaagccct cggcatcgcc cacgaggtgg
     5521 aagtgatcgc agaatggtat ttgaaactgc tcgctacagg aaaacctatc ccaacgctaa
     5581 acaaagaaca aatggatatc gtactcggaa aatttaagtc ctatggttct tgggtggaaa
     5641 gcgaacaata gcctgccata aataacatac aagcggtgat atttcttaaa atttttacaa
     5701 acggagtgca gtatgcaaaa tgaagtaata ctcacgatga aaaacatcag taaatccttt
     5761 gcgggggtca atgcgttaaa aggtgttgag cttacgctgc gtaagggcga agttcacgca
     5821 ttaatgggcg aaaacggtgc aggtaaatcc accttgatga aatgcttgct gggcgtatat
     5881 caagccgatg aaggtgaaat catatataaa ggtcagcctg ttcgctttga gtcggtatta
     5941 gaggcacaaa aagcgggcat tagtatgatt ttccaagaac tgaacctcat tccccattta
     6001 acggtggcag agaatatttt cttcgcccgt gagccactca aacacggttt agtggataaa
     6061 gacaaaatgg tggaagaatc ggctaaattg ctgaatttct ttgagattga tgttgatcca
     6121 aacgatgaag tgagaatgtt gtcggtagcc aaacaacaga tggtagaaat tgccaaagcc
     6181 ctttcatttg atgtggaagt actgattatg gacgagccga cctccgctct taccgaaaaa
     6241 gagatcgata aattatttga attggtcgat agactgcgtg ccaaaggggt gagcattgtc
     6301 tatatttctc accgaatgga agaattaaaa cgtatctgta accatattac gattttccgt
     6361 gatggcacct atgtatctaa ccataaattc aatgaaatca cgatggatga gatcattacc
     6421 aaaatggttg ggcgttcatt ggataaccat ttcccaccaa aaaccgctgt ggtgacaaac
     6481 gacattatcc tctcgattat gaatgctgaa cgtaagggag tgtttgaacc attgaacttt
     6541 gacttacgca aaggagaaat tctgggcatt accggcttgg ttggagcaaa acgtactgaa
     6601 ctggctcgag ccatcttcgg agcagaccca cttgatagcg gtgagatttt tgtgcacgag
     6661 aacaaagtca gcattaaaga tccgagtgat gcaattaaag ccggtatagc ctatttgtcg
     6721 gaagaccgta agctcaatgg tgttgcagta agaatgtcga ttcgtgagaa tatcacgatg
     6781 gcatctatgg ataaagttgc caatcaaata ggtgtgattt cttacgatga agaagaaaaa
     6841 gcctcaaaaa ctttcatcga caaaatggaa atcaaaaccc caacgattga gcagaaagtt
     6901 caaaacttaa gtggtggtaa ccaacaaaaa gtggtaattg gtaaatggtt attccgtgaa
     6961 gcaaaagtga tgatttttga tgagccgact cggggaattg atgtcggagc aaaatatgct
     7021 atctatcaac tgctcgatga gcttgctgcc aatggcgtgg gtgttatcgt gatctcttcc
     7081 gaattgcctg aagtcttggg agtttccgac cgcatcattg taatgcgtga aggtagaatg
     7141 acaggtatgc tcgagactaa aaaaaccaac caagaagaaa ttatgcatta tgcaactggt
     7201 gttaaaaata tgtttgctcg tgaatatggg gtggaaaaat gaatgaaaaa caaaaagaaa
     7261 tgttaagaaa aatggctgca ttggctggct tagtactatt aatcatcttt ttcagcgtaa
     7321 ccaatgaata cttctttacc tccaataaca ttatgacagt agggctacaa acctctacca
     7381 ttgcgttaat tggtattggg gcaacctgtg tgatcttaac cggtggaatt gatttaagta
     7441 ccggttcagt agttgcactc tccggtgtgg cagctgcaat gattgtaaat gccggtgtac
     7501 ccgtaccact aggaatgatt ttagggattt tggtcggcgg tatttgtggc ttaatcaacg
     7561 gcattttagt caccagaatg aaattgcctc cgtttattgc taccctcggt atgatgatgg
     7621 ttgcccgtgg tttagcattg tatgttacca atgccgcacc agtttccgga atgcctgaat
     7681 cctttgccaa tctcggtaac ggagcattat ttaaaattgt ggaagaaggg gcaaacggct
     7741 tgccgaaagt ggtatttgca ggtattcctt accctgtgat tattatgatc tttattaccg
     7801 tactattcac tttcgctctc tctaaactga aagtcggacg ctatctttac gcaattggtt
     7861 ctaacgaaga agccgcacgc ttatccggca ttaaaaccaa tattgtcaaa atctatgcct
     7921 atgttgccag tggcttactc tctggtttaa ctggcgttat ccttgcctcc cgcctcgtca
     7981 ccgcccagcc aaacggtggg gtctcttacg agctggatgc gattgccagt gctgtggtgg
     8041 gcggcacatc gcttatgggc ggtgtgggta ctatccccgg cacactcatc gggtcattca
     8101 ttatcggggt actccgtaat ggtttgaata tgaatggcgt ttcttcattt gttcaaatga
     8161 ttgtcatcgg gcttgtgatt atcgtggcgg tctctctcga ccagctccgc caatccaaaa
     8221 aagccggtaa aaaataatcc actcttaaag gagaattaaa atgaaaaaat cagcattatt
     8281 tactgcgacc ctctttggtt tatcgacctt atttgcaggc agtgcaatgg caaaatctga
     8341 tgaaatcgct gtgattgtga aatctgccaa ctctaccttc tggcaaaacg tgcgtaaagg
     8401 ggctgaaact gccggcaaag atattggagg acaatacaaa gtcaccttcc aagggccgga
     8461 agccgaaaca gcgattgacg cccaagtgaa tatggtggac aacgccgtaa accgtggcgt
     8521 ggcaggcatc gtactggctg cctcagaccc ggtagcatta gtgccggcgg tgaaaaaagc
     8581 ttatgagagt ggcattccgg tggtgcttat cgattccggc atcaacagtg acggcaaata
     8641 ctaccaatct ttccttgcca ccgacaaccg tgcagcaggt aaattagcag cggaaaaact
     8701 gcttgccaaa gtcaaaggcg gcaaagtggc agtgatgagc tttacccaag gggcagcatc
     8761 agcgattgag cgtaccggtg gttttattga cgaaatcaaa gcaaaagccg attacaaact
     8821 ggtcggcccg tactactccg attcggatat gaccaaagcg ttaaaccaaa ccgaagacgt
     8881 attaggttct aaccctgatc ttgcagcgat cttcggggcg aacgaaccga ctgcggtagg
     8941 aatggcacga gcggtgaaac aaaaaggctt tgccggcaaa attgtagcgg tgggctttga
     9001 tggaaactcc gacctacaaa acttcgtgcg tgacggaaca ctagacggca tcgtggtgca
     9061 atcttcctac caaatgggct ataaaggcgt ggacaccatc ggcaagctga ttaaaggcga
     9121 gaaagtcgaa aaagtgattg ataccggggt ggtttatgtc acgaaagaaa acattgatag
     9181 cgaagaagcg aaaggtgttc tttactaaca ttcaagttaa acggggtggg gttgcccacc
     9241 ctttcagccg aaaggaagcc aatatgtcaa atacccttac cgataaagcc ttagtggtga
     9301 ttgccgcact tggcggtcga gaaaatattg tcgatgttta cgcctgtatt acccgcctac
     9361 gtgtggaggt gaaaaatacc gacattgtgg aatgggataa aattaaatct gtcggtgcgt
     9421 taggcgttgt gacctcaggc tcagttattc aatctatttt tggtagcgat gccgaagcct
     9481 accgtgatga aatcaaacgt attttgaata tttagcctaa gcacactcac aacaaaagaa
     9541 tcgcaacaag cggtgaaatt tttatcaaaa tttgcaaaaa aatgacgtaa tttgaccgct
     9601 tgcaagttaa tcgaattgtt taacttttaa gaaggaaaat agtatggcaa atcgtcttat
     9661 cctcaatgaa accagttacc acggcaaagg agcaatccaa aatattgttc acgaagtgac
     9721 cagccggagt ttcaaaaaag cccttgtggt aacagacaaa gacctgatca aatttaacgt
     9781 tgcccaaaaa gtcaccgcct tgcttgatgc cgcaggcttg ccttatgaga ttttcgatga
     9841 ggtcaaagcc aacccggcag tcgatgtgat caaagcgggg gtcgcaaaat tcaaagcctc
     9901 cagtgccgat tatttaattg cgattggtgg cggctcaccg attgatagtg ccaaagcgat
     9961 tggtattatc atcaataacc ctgagtttag cgatgtactt tcattggaag gtgtcgcccc
    10021 aacccaccaa aaatgtgtgc cgattatcgc cattccaact accgcaggta cagccgcaga
    10081 agtcacgatt aactatgtca ttaccgatga agcgaaaaaa cgcaaatttg tgtgcgtgga
    10141 cgttcacgat gtgccggcag tggcgttagt cgatccagat atgatggaat ctatgccgaa
    10201 aagcttggct gccgcaaccg gtatggacgc tctcacccac gccattgaag gctacattac
    10261 caaagcggcg tgggaattaa ccgatgctct acaccttaaa gcgattgagc tgatcggccg
    10321 ttcattgcgt ggtgcagtgg agaaaaatcc gaaagatgtc gaggcaatgg cattgggaca
    10381 atatgtggct ggaatgggct tctccaacgt tgggcttggc gcggttcaca gtatggcaca
    10441 cccgctttct gcctattacg acaccccaca cggcattgcc aacgcagtat tactgccgta
    10501 tgtgatggaa tttaataaaa attacaccgg cgaaaaatac cgtgaaattg cccgagcaat
    10561 gggggtaaaa ggcgtagatg aaatgagcca agaagagtac cgcaacgccg ccattgctgc
    10621 cgtacaacag ctttcacaag atgtcggcat tccgccaaaa ctgcatcaaa tcggcgttaa
    10681 agaagccgat ttagcggcat tatcggtcga tgcctttaat gatgtctgca ccggtggcaa
    10741 cccgagagat tgtacggctg aggagctgtt ggaggtatat cggatcgcat actaagttct
    10801 atctttgtta aatagacaag cggtgatttt ttaataaaaa tttgcaattt ctgttaaaaa
    10861 aatactgata gtcataatgc ccgatttctc atatttatgg aaaaatcggg cattttcgtt
    10921 gcttttttat cgc
//
LOCUS       DANLZC010000085        10879 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.085, whole genome shotgun sequence.
ACCESSION   DANLZC010000085 DANLZC010000000
VERSION     DANLZC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.085"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(56..1771)
                     /gene="proS"
                     /locus_tag="PXE44_001677"
     CDS             complement(56..1771)
                     /gene="proS"
                     /locus_tag="PXE44_001677"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL5589177.1"
                     /translation="MRTSQYLFSTLKEMPNDAQVVSHQLMLRAGMIRPMASGLYNWLP
                     TGIKVLKKVENIIREEMNKGGAIEVLMPVVQPAELWVESGRWNDYGPELLRFKDRGER
                     DFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDA
                     YSFHTTQESLQETYDVMYQVYSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGE
                     DDVVFSTESDFAANIELAEAVAVGERQAPTAEMQLVDTPNAKTINELVENHGLAIEKT
                     VKTLVVKGATEEQPLIALVIRGDHELNEIKAQKHPLVADPLEFADEAEIKAKIGAGVG
                     SLGVVNMPIPVIIDRSVALMSDFGCGANIDGKHYFNVNWERDVAVPEIADLRNVVEGD
                     PSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVV
                     AAAIEQHYDDRGIIWPCDEMAPFTVAIVPMNMHKSEKVQAFAEELYANLKAQGVEVIF
                     DDRKERPGVMFADMELIGVPHMVVIGEKNLENGEIEYKNRRSGEKQMIAKESLLAFLK
                     ANVKA"
     gene            complement(1935..2096)
                     /locus_tag="PXE44_001678"
     CDS             complement(1935..2096)
                     /locus_tag="PXE44_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589178.1"
                     /translation="MEQKLNNVFGGIMFKEKDYDEYLEAKVKKGQIFTLEQANAEWQE
                     IIEQIEREN"
     gene            complement(2149..4032)
                     /gene="htpG"
                     /locus_tag="PXE44_001679"
     CDS             complement(2149..4032)
                     /gene="htpG"
                     /locus_tag="PXE44_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDL5589179.1"
                     /translation="MANNQETRGFQSEVKQLLQLMIHSLYSNKEIFLRELISNASDAA
                     DKLRFKALSEPALYEGDGELRVRVSVDETLGTITIADNGIGMTREQVIDHLGTIAKSG
                     TKEFLNALGTDQAKDSQLIGQFGVGFYSSFIVADKVTVKTRAAGVPAEQGVIWESAGE
                     GDYTVADLEKKERGTEIILHLREDEKEFLNEWRLREIIGKYSDHIGLAVEIQTNEYDD
                     EGKASGQKWEKINKAQALWTRSKNEISDEEYTEFYKHLSHDFADPLIWSHNKVEGKQE
                     YTSLLYVPSKAPWDLFQREQKHGLKLYVQRVFIMDDAEVFMPNYLRFMRGLLDTNDLP
                     LNVSREILQENKVTQSLRSALTKRALQLLEKLAKDDAEKYAQFWQAFGVVLKEGVGED
                     FANKQQVAGLLRFASTQRDDSEQAVSFADYIARMKEGQKAIYFLTADSYQAAKNSPHL
                     ELFHKKGIEVLLLSDRIDEWLVSHLTEFDGKPLQSITKSDLDLGELADKQEEESQKAQ
                     QAEFGSFLERAKGYFGERVKNVVLTHRLTDTPAVVSTDSDEMTTQMAKLFAAMGQQAP
                     EVKYTFELNPDHPMVKKVADLADEAEFADWIELLFEQALLAERGTLENPTAFIKRVNK
                     LLG"
     gene            4196..4483
                     /locus_tag="PXE44_001680"
     CDS             4196..4483
                     /locus_tag="PXE44_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL5589180.1"
                     /translation="MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDY
                     QQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVEIRKSNKPRPVDFGDE"
     gene            4483..4911
                     /locus_tag="PXE44_001681"
     CDS             4483..4911
                     /locus_tag="PXE44_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL5589181.1"
                     /translation="MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCIT
                     LSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQTKAFVVNNDIALLASEYHIPNKM
                     DLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA"
     gene            4915..6996
                     /gene="recG"
                     /locus_tag="PXE44_001682"
     CDS             4915..6996
                     /gene="recG"
                     /locus_tag="PXE44_001682"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL5589182.1"
                     /translation="MSEQLLDGVPLTALSGVGAAISEKLSRIGINNVQDLLFHLPMRY
                     EDRTRITPIADVRPESFATIEGYVQLTEVQFGKRPILSVTLSDSTSKIMLKFFNFNAG
                     MKNSLATGVRVKAFGEIKRGRFMAEIHHPEYQIIRDNQPLVLAETLTPIYSTTEGLKQ
                     ASLRKLTEQALALLEKVKVAELLPDEYNPHKYSLKEALQLLHRPPPTISAEVLEKGDH
                     PAQKRLIFEELLAHNLAMQQLRLGVKQQYAEALTYQTDIKQRFLDSLPFKPTHAQSRV
                     TADIEQDLAKPHPMMRLVQGDVGSGKTLVAALAALLAIDNGKQVALMAPTEILAEQHA
                     NNFANWLNPFGIEVGWLAGKVKGKARTAQLEAIKNGDVQMIIGTHALFQDNVEFHHLA
                     LVIIDEQHRFGVHQRLTLREKGAKENNYPHQLIMTATPIPRTLAMTVYADLDTSIIDE
                     LPPGRTPITTVVISEDRRAEIVQRVYQVCKQEHRQAYWVCTLIDESEVLEAQAAAAIA
                     EDLQRALPDLRIGLVHGRLKPQEKQAIMAEFKAANLDLLVATTVIEVGVDVPNASLMI
                     IENSERLGLAQLHQLRGRVGRGATASHCVLMYKPPLGKISSKRLQVMRDSQDGFYIAE
                     KDLEIRGTGEILGTKQTGMAEFKVADLMRDRKMIPLVQRYAKQIISENQPLAEALIKR
                     WLDNKAEYTNA"
     gene            complement(7018..7952)
                     /locus_tag="PXE44_001683"
                     /pseudo
     CDS             complement(7018..7952)
                     /locus_tag="PXE44_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
     gene            complement(8005..9657)
                     /locus_tag="PXE44_001684"
     CDS             complement(8005..9657)
                     /locus_tag="PXE44_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL5589183.1"
                     /translation="MTIFQVAQNWLAQDPDAETRAELEQLIQAAESDEKAKAELTARF
                     DGRLQFGTAGLRGRLQAGSMGMNRVLVAQAASGLAGFIKGYDKEPSIVIGYDGRKNSD
                     VFARDTAEIMAAAGIKTYLLPRKLPTPVLAYAIQYFDTTAGVMVTASHNPPEDNGYKV
                     YLGKANGGGQIVSPADKEIAALIDKVAAGDIRDLPRSQDFTVLDDEVVNAYIEKTASL
                     AKRPKAEINYVYTAMHGVGYEVLSKTLEKAGLPQPYLVSEQIQPDGSFPTVNFPNPEE
                     KGALDLAIKLAKEKNAEFIIANDPDADRLAVAVPDAQGNWKPLHGNVIGCFLGWYLAK
                     QFHAQGKQGVLACSLVSSPALAEIAKKYGLSSEETLTGFKYIGKVENLLFGFEEALGY
                     LVDPDKVRDKDGISAAIMFLDLVCSLKQEGKTLADYTTEFVQEFGAYVSGQISIRVCD
                     LAEIGKLMTALRNNPPSEIGGFKVAEFIDHTKTTRQNDILVFVLENGSRLIARPSGTE
                     PKIKFYLDARGTDAENAEQVLAQFDESVRSLLRQEEYGKQDC"
     gene            complement(9809..10870)
                     /locus_tag="PXE44_001685"
     CDS             complement(9809..10870)
                     /locus_tag="PXE44_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5589184.1"
                     /translation="MAIYAIWNNKGGVGKSYLTFQIACEYAKQYPAKKVLVVDLCPQA
                     NSSSMLLGGIEKGEQELAKIHSQVNKNTISGYISERLVSPYRSINSATKYVTQVSTIN
                     EYVPKNVYLVVGDEELEIQSSGILHASQAVHVPDSWRLVHLWVRDLVSDIQNAWDNDD
                     NVVFIDCNPSFTIYTELALAASDRLIIPFSADGSSKRAVKSVLSLVYGVKRHSGDVSS
                     HFYTESQKSKLALPQIYMYVGNRLTINAKASATAFKTVVEEIGDEIYNIWKTNANLFC
                     IHPAGVGTPTNKSSFRKMFQYEVRDANTASVVSGALGIPISRLSSGKRDVLGKNVMVN
                     QSQLDAQVPNIQNLVQCIE"
BASE COUNT         2986 a         2567 c         2230 g         3096 t
ORIGIN      
        1 ctcgctgctt tatattaaag ggcgtatttt atacgcccaa ttttgttatt tataattaag
       61 cctttacatt cgcttttaag aacgcaagta agctctcttt ggcgatcatc tgtttttcac
      121 cgctgcggcg gtttttgtat tcgatttcgc cgttctcgag gtttttctca ccaataacca
      181 ccatatgcgg cacaccgata agctccatat ccgcgaacat cacgcccggg cgttctttac
      241 gatcatcaaa aatcacctca acgccttgag cttttaagtt agcgtataac tcttcagcga
      301 atgcctgcac tttttccgat ttgtgcatat tcatcggcac aatcgccacc gtaaatggcg
      361 ccatttcatc acaaggccag ataataccac gatcatcata atgttgttca atcgcagctg
      421 ccaccacacg ggtaacgccg ataccgtaac aacccattgt catcactagc ggtttaccgt
      481 cttcaccttg cacagtggct ttcattgctt ctgagtattt tttgcctaat tggaaaatat
      541 gccctacttc aatacctcgt ttgatttgaa gtgtgccttt accgtccggg cttgggtcgc
      601 cttccaccac attgcgtaaa tctgcaattt ccggcacagc aacatcacgc tcccagttca
      661 cgttaaagta gtgtttgcca tcaatattcg caccgcagcc aaaatctgac atcaacgcca
      721 ctgagcggtc aataatcacc ggaatcggca tattcaccac acctaacgag ccaacaccgg
      781 caccgatttt ggctttaatt tccgcttcat cggcaaactc aagcggatca gccaccaacg
      841 ggtgtttttg agctttaatt tcgtttaact cgtggtcgcc acgaatcact aacgcaatta
      901 acggttgctc ttcagttgcc cctttcacca ctaaggtttt caccgttttt tcaatggcta
      961 aaccgtgatt ttctactaat tcattgatgg ttttcgcatt tggcgtatcc actaattgca
     1021 tttctgccgt tggtgcttgg cgttcaccca ccgcaaccgc ttcggctaat tcaatattgg
     1081 cagcaaaatc tgattcggtt gaaaatacca catcatcttc accgcttgag gctaacactt
     1141 ggaactcgtg agaggctgaa ccgccgatag agccggtatc tgcctgcacc gcacggaaat
     1201 ctaagcccaa acgggtgaaa atattgctgt aaacctgata catcacatca taggtttctt
     1261 gcaggctctc ttgtgtggtg tggaaagaat aggcatcttt catcacaaat tcacgcgaac
     1321 gcattacccc gaaacgaggg cggacttcat cacggaattt cgtttgaatt tggtataaat
     1381 tcagcggaag ttgtttgtac gaagaaactt cacggcgaac caagtcggta atcacttctt
     1441 cgtgagtcgg gccaagcacg aaatcacgct cgccacgatc tttaaaacgt aataattccg
     1501 ggccgtaatc attccaacga ccggattcca cccataattc cgccggctga accaccggca
     1561 ttaatacctc gatagccccg cctttgttca tctcttcacg aataatattt tccacttttt
     1621 tcaacacttt gatcccggtc ggcaaccagt tgtataaacc tgacgccatc gggcgaatca
     1681 ttccggcacg caacattaat tgatggctaa ctacttgtgc atcattcggc atttctttaa
     1741 gggttgaaaa taaatattga cttgtacgca ttgctattcc tattcttttt aaactttgaa
     1801 acctgaaaaa atctgcttat tttaaaacaa agcggttaaa ttttcaggaa attttgcaaa
     1861 atcctataaa aaaataaccg cttgtaagcg gttattttgt tttaactaag ggtattttct
     1921 taaactgtgt atgcttaatt ttctcgttca atttgctcaa taatctcttg ccattcggca
     1981 tttgcttgtt caagagtaaa aatctgacct ttttttactt tagcctctaa atattcatca
     2041 taatcttttt ctttaaacat tattcccccg aatacattgt ttaatttttg ttccattata
     2101 tattgaatat ggaaaaacgg aaacctagat ttccgttttt agtaatggct agcccaacaa
     2161 tttatttacc cgtttaataa aggctgtcgg gttttccaac gtgcctcgtt cagcaagcag
     2221 agcttgttca aacagcagtt caatccaatc ggcgaactcg gcttcatcgg caagatcagc
     2281 tacttttttc accattgggt gatctgggtt cagctcaaaa gtatatttca cttctggtgc
     2341 ttgttgcccc attgctgcaa acagttttgc catttgggtg gtcatttcat cgctgtcagt
     2401 ggaaaccact gccggtgtgt cggttaagcg gtgagtcagc accacgtttt tgacccgttc
     2461 accaaagtag cctttcgccc gttctaagaa cgaaccaaac tccgcctgtt gggctttttg
     2521 gctctcttct tcctgcttgt cggcaagctc gcctaagtct aagtccgact tggtaatgct
     2581 ttgtagcggt ttgccgtcaa actcggtgag gtggctaacc aaccattcat caatgcggtc
     2641 agaaagcagt agcacctcaa tgcctttttt gtggaacagc tctaagtgcg ggctgttttt
     2701 agccgcttgg tagctgtctg cggttaagaa gtagatcgct ttctgcccct ctttcattct
     2761 tgcaatataa tcggcaaaac tgaccgcttg ttcgctgtca tcacgctggg tggaggcaaa
     2821 acggagtaag cctgcaacct gttgtttgtt ggcaaagtct tcgcccacgc cctctttaag
     2881 caccacgccg aacgcttgcc agaattgggc atatttttcg gcatcgtctt tcgccaattt
     2941 ttccagcagt tgtaacgcac gcttggttaa ggcagaacgg agcgattggg taactttatt
     3001 ctcttgcaga atttcacggc tgacgttgag cggcaggtcg ttggtgtcga gcaagccacg
     3061 cataaaacgc aagtagttcg gcataaagac ttccgcatcg tccataataa atacacgctg
     3121 aacgtagagt ttcaagccgt gtttttgctc gcgttggaac agatcccacg gggctttgct
     3181 cggcacatac agcaagctgg tgtattcttg cttaccttcg actttattgt gcgaccaaat
     3241 cagcgggtcg gcaaaatcat ggctgaggtg tttgtaaaat tcggtgtact cctcgtcact
     3301 aatctcattt tttgagcgtg tccaaagcgc ttgagctttg ttaatttttt cccatttttg
     3361 accgcttgct ttgccctcgt catcgtattc attggtctga atttccaccg ccaggccgat
     3421 atggtcggag tatttgccga taatctcacg caaacgccat tcgtttaaaa actctttttc
     3481 gtcctcacgc aagtgaagaa tgatttccgt gccacgctct tttttctcca gatcggcaac
     3541 ggtgtaatcg ccctcaccgg cagattccca aatcacgcct tgctcggcag gcactccggc
     3601 ggcacgggtt ttgactgtca ctttatccgc cacgatgaaa gaagagtaaa agcccacgcc
     3661 gaactgcccg ataagctggc tgtctttggc ttgatcagtg ccgagtgcgt tcaaaaattc
     3721 tttggtacca gattttgcaa tcgtaccgag atggtcgatc acctgctcac gggtcatacc
     3781 gataccgtta tcggcaatcg taatcgtacc gagtgtttca tcaacagaga cacgcacacg
     3841 cagttcgcca tcaccttcat acaaagccgg ctcggaaagg gctttaaacc gcaatttatc
     3901 ggcggcgtca gaggcgttgg aaataagctc acgcaggaaa atttctttgt tggagtaaag
     3961 cgagtggatc atcagttgca ataactgttt cacttccgac tggaagccac gggtttcttg
     4021 attatttgcc attgtgtatt cctttattca ttaaaaaaca gggaatagat ggggcaagtg
     4081 gtcagatttt caagattttt tgcaaaatat ctgttgccaa atagcgaatt aattgaaaga
     4141 tattactaca tttaataaaa tacactcatc tttttactac attttggaga gcgatatggc
     4201 aataaccatt acttccagag aatttaatca aaatgccagc aaagtgttaa aaatggcaga
     4261 aaaagagccg gtttttatta ccaaatgggg caaaatcgtc aatgtggtgt ctagttatat
     4321 ggattatcag cagaaaaagc ctagcgagcc aagctttgaa gagctattcg cctcttccga
     4381 aaatgaggat gtgagcgatg aattttggca agagtttgaa caaacattgg ttgaaatccg
     4441 caaaagcaat aaaccaagac ctgttgattt tggagatgag taatgtatcg ctaccttttt
     4501 gataccaata tagtttccga gctttataag ttaagaaatg acaagatgga tgtcaatgtt
     4561 cgccagtggc tgagaggggt taatcctagc cagaccaata tcagctgtat tacgttatct
     4621 gaaatcaaaa caggtatttt gctgaaagcc cgcaaagaca aagaacaatc agaccgatta
     4681 aatgattggt ttgaaaataa tgtattaaag gcttatcaaa caaaagcgtt tgtggtgaat
     4741 aacgacattg ctctattggc atcagagtac catattccca ataaaatgga tttaaatgat
     4801 gcttatattg cggcaaccgc caaataccac aatctgattt tagtcacccg caacaccaaa
     4861 gattttatcc gttgtgatgt gcgtttattt aacccatttg aaaccgcata aataatgagt
     4921 gaacaattat tagacggcgt gccattaacc gccctttccg gcgtaggagc ggcaatttcc
     4981 gaaaagttaa gtcgaatcgg cattaataat gtgcaggatt tactgtttca tctgccaatg
     5041 cggtatgaag atcgcacccg tattaccccg attgctgatg ttcgcccgga aagctttgcc
     5101 accattgaag gctatgtaca acttaccgaa gtccagtttg gtaaacgccc gattttatcg
     5161 gttacactct ccgacagcac cagcaaaatt atgcttaaat tcttcaattt caatgctgga
     5221 atgaaaaata gccttgccac cggcgttcgg gtaaaagcct ttggggaaat taagcgtggg
     5281 cgatttatgg cggaaattca ccaccctgaa tatcaaatta ttcgggataa tcaaccgctt
     5341 gtactggctg aaaccctgac cccgatttac tccaccactg aggggttaaa acaagcctcg
     5401 ctgcggaaat taaccgagca agccctcgct ttattagaga aagtgaaagt cgccgagctg
     5461 ttgcctgatg aatacaatcc gcacaaatac agcctaaaag aagcgttgca gttattgcac
     5521 cgcccaccgc cgacgatttc tgccgaagtc ttggaaaaag gtgaccaccc tgctcaaaaa
     5581 cggctgattt ttgaagaact gctggcacac aatttagcga tgcagcaact ccgtttaggg
     5641 gtaaaacagc aatatgcaga ggcacttact taccaaaccg atataaaaca gcgttttctc
     5701 gacagtttgc cttttaagcc tactcacgca caaagccgag taaccgcaga tattgagcaa
     5761 gatcttgcca aaccacaccc gatgatgcga ctggtacaag gcgatgttgg ttcaggtaaa
     5821 accttagtag ccgccttggc agcactatta gcgattgata acggcaagca ggtggcatta
     5881 atggcaccga ccgaaattct tgccgaacag cacgccaata attttgccaa ctggcttaat
     5941 ccctttggga ttgaggttgg ctggcttgcc ggtaaagtga aaggcaaggc gagaaccgca
     6001 caacttgagg caatcaaaaa cggtgatgtg cagatgatta tcggcactca cgccctgttc
     6061 caagacaatg tggaatttca ccatttagcg ttggtgatta ttgatgaaca acaccgtttt
     6121 ggcgtacacc aacggctgac cttgcgggaa aaaggagcga aagaaaataa ctatccgcat
     6181 cagctcatta tgacggcgac ccccattccg agaacgctgg caatgacggt ctatgctgac
     6241 ctcgacacct caatcattga cgaactgcca ccgggtagaa cgccaatcac gactgtggtg
     6301 atttctgaag atcggcgagc agaaatcgtc caacgggtgt accaagtctg taagcaagaa
     6361 caccgccaag cctattgggt ctgcaccctg attgatgaaa gcgaagtgtt ggaggctcaa
     6421 gccgcggcag cgattgccga agatttacag cgagccttgc cggatttacg catcgggtta
     6481 gtacacggac ggctgaaacc acaggaaaaa caagcgatta tggcggaatt taaggcagcg
     6541 aatttagatt tgctggttgc gaccaccgta attgaagtgg gagtggacgt gccgaatgct
     6601 agcctaatga ttatcgaaaa ttccgaacga ctcgggctgg ctcaactcca ccagttgcgt
     6661 ggacgtgttg ggcgaggggc aaccgcctcg cattgcgtct taatgtacaa accgccgctc
     6721 gggaaaattt ccagcaagcg gttacaagtg atgcgtgata gccaagacgg tttctacatt
     6781 gccgaaaaag atttggagat tcgaggcaca ggcgaaattc tcggcacaaa acagaccgga
     6841 atggcagaat ttaaggtggc ggatttaatg cgagaccgca aaatgattcc gctggtgcaa
     6901 cgttacgcca agcaaattat tagcgaaaat caaccgcttg cagaggcgtt aattaagcgt
     6961 tggttagaca acaaggcgga atataccaac gcttaaccgg caagcgggtt atttagatta
     7021 cggcaggttt ttcaccgatt caattaccaa atcccaaaag cgggatttgt ctaatttttt
     7081 tgctacccaa gtattacact gttttggcgg gtagcggaaa tccgccaccg tcatacccag
     7141 tgtatgcgtg cctgttagct caatattgac cggcacttgc tgaacttcaa tcaggcttgg
     7201 gtcaatcaca tacgccaccg cacaggcatc gtgaaccggt ggagcatcaa agccttgagc
     7261 ctgtttatac attttggcga agaacgccag caattccacc acaaactgcc ccgatttgct
     7321 gtttaatgcc ttaaagcgtt caaccacatc gggggtcgca agtgcttggt gagttaagtc
     7381 taagccgacc atcgtcacct tccaaccggc attaaacaca atatgagcag cttccgggtc
     7441 aattttaata ttaaattctg ccaccgcact ccagttgccg gtgtgataac ctccgcccat
     7501 cagcaccact tctttcactc tttcaataat acggggctca aggcgggcag ctagagcaat
     7561 attggttatt gcccaccggt cggcactaag gtaatgcttt tctccgggtg cgacattatc
     7621 aaatcaataa ttaattgagc ggcgtgtttt ttctcaaatt gtcgggtaga ttcagggagt
     7681 acaggcccat ccattcccga atcaccgtga atatccgggg cattttccac ttcacgcacc
     7741 agcggtctgg cacaaccttt ggcaaaagga acgttgcaaa tatgagcaat ttccgcaacc
     7801 gccaacgcat tacgcgatac tttttctaag gtttgattac ctaccaccgt agtcactgcc
     7861 aacaaatcaa tattcggatt cccccaagcg agcatcatcg caatcgcatc gtcatgcccc
     7921 ggatcacaat ctaaaataat tttttgagcc attattgtct cctttaaaaa agggagagcc
     7981 taagctcccc ctcaattaat gccattagca atcttgtttg ccgtattctt cttggcgaag
     8041 cagcgaacgc acactttcgt caaactgagc cagcacttgc tctgcgtttt cagcgtctgt
     8101 gccacgagca tctaaataga atttaatttt cggctcagta cctgaagggc gagcaatcaa
     8161 acggctgcca ttttccaaca caaacactaa aatgtcattt tggcgtgtgg ttttggtatg
     8221 gtcaataaac tcagccactt taaagccacc aatttcgctt ggaggattgt tgcgtaacgc
     8281 cgtcattaat ttgccgattt cggctaaatc acaaactctg atcgaaattt gaccgcttac
     8341 ataagccccg aactcttgta caaactcggt ggtgtaatcc gcaagggttt taccttcttg
     8401 tttgaggctg cacactaaat ctaagaacat aatggcagcg gaaatgccgt ctttatcacg
     8461 cactttgtct gggtcaacca aataacctaa ggcttcttca aagccaaata ataggttttc
     8521 aacttttccg atatatttaa agccggttaa ggtttcttcc gagcttaaac catatttttt
     8581 cgcaatttca gctaacgccg gagaggatac taacgagcaa gccaacacgc cttgtttacc
     8641 ttgtgcgtgg aattgtttgg ctaaatacca gcctaagaaa cagccgatca cattgccatg
     8701 cagtggtttc cagttgcctt gagcatcagg cacggctacc gctaaacggt cagcatccgg
     8761 gtcgttggcg ataataaatt cggcattttt ctctttcgcc aatttaatcg ctaaatctaa
     8821 cgcccctttc tcttccgggt tcgggaaatt aaccgttggg aacgaaccgt ccggctgaat
     8881 ctgctcacta actaaatacg gttgcggtaa gccggctttt tctaatgttt tgcttaacac
     8941 ttcatagccc acaccgtgca ttgcagtgta aacgtagttt atttcggctt ttggccgttt
     9001 ggctagtgag gcggttttct caatataagc atttaccact tcatcgtcta acaccgtgaa
     9061 atcttggcta cgaggtaaat cacgaatatc gcctgctgct actttatcaa tcagagcagc
     9121 aatttcttta tctgccggcg aaacaatttg tccgccaccg ttggctttac ctaaataaac
     9181 tttatagccg ttatcttccg gtgggttgtg gctggcagtt accatcacac cggctgtggt
     9241 atcaaaatat tgaatcgcat aggcaagcac tggtgttggc aatttgcgag gaagcaggta
     9301 cgttttaatg cctgccgctg ccataatttc agcggtgtca cgggcaaaca catcggaatt
     9361 tttacgaccg tcataaccaa taacaattga cggctcttta tcataacctt taataaaccc
     9421 agctaaaccg ctggcagctt gtgccaccaa aacacggttc atacccattg aacccgcttg
     9481 taagcgacca cgcaaaccag ccgtaccaaa ctgtaaacgt ccgtcaaaac gggctgttaa
     9541 ttctgctttt gctttctcgt ccgattctgc cgcttgaatt aactgctcta attccgcacg
     9601 ggtctctgca tccgggtctt gggctaacca attttgtgca acttggaaaa tagtcatttt
     9661 ttactcctat gtaatttaaa aactctgttt agattagccc aaagtccttt tatttaaaag
     9721 gagatatttc tttttttgtg atcgagctat caaataaaaa gatttgtcag aaataaaaaa
     9781 tagcgtactt aatatacgct atttttcttt attctataca ctgaacaaga ttttgaatgt
     9841 taggaacttg agcatctaat tgtgactgat tcaccattac atttttacct aaaacatctc
     9901 tttttcctga tgataagcga ctaattggaa tacctaaagc acctgaaaca acagatgctg
     9961 tatttgcatc tctcacctca tactgaaaca tttttctgaa gcttgattta tttgtaggag
    10021 taccaacacc tgctgggtga atacagaaca aattagcatt agttttccat atattataga
    10081 tttcatcacc tatttcctct acaactgttt taaatgcggt tgctgatgct ttggcattta
    10141 tcgttaatct attacctaca tacatataaa tttgaggtaa tgctaactta ctcttttgag
    10201 attctgtata aaaatgagag ctgacatccc cagagtgcct tttaacccca tatactagag
    10261 ataatacaga cttaacagct cgttttgatg aaccatcagc ggaaaaaggg atgatcaatc
    10321 tatctgaagc agccaacgct aattcagtat aaatagtgaa acttggattg caatcaataa
    10381 atacaacgtt atcatcatta tcccaagcgt tctgtatatc agaaactaaa tcacgcaccc
    10441 aaagatgcac tagccgccaa gaatcaggaa catgaacagc ttgggaagca tgtaaaatac
    10501 cagatgattg gatttctaat tcctcatcac caacaacaag ataaacattt tttggtacat
    10561 attcatttat agtgctaact tgagttacat atttcgttgc actattaata gaacggtaag
    10621 gacttactaa tctttctgaa atatatcctg aaatagtatt cttgtttact tgagaatgaa
    10681 tttttgctaa ctcttgctca cctttttcaa tgccgcctaa tagcatagat gatgaatttg
    10741 cttgaggaca taaatcaaca acgagaactt tttttgctgg gtattgtttt gcatactcac
    10801 aagcgatttg aaaagttaaa taacttttac caaccccacc tttattattc catattgcat
    10861 aaatagccat aatgccccc
//
LOCUS       DANLZC010000086        10878 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.086, whole genome shotgun sequence.
ACCESSION   DANLZC010000086 DANLZC010000000
VERSION     DANLZC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.086"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(76..381)
                     /locus_tag="PXE44_001686"
     CDS             complement(76..381)
                     /locus_tag="PXE44_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDL5589185.1"
                     /translation="MRKPAHPGQILLDGFIEPNNIKIKELADHLGFSRETLSRVLHGK
                     TPMTANLALSLEEAGISTAKLWLNLQTKYDLWELKQQRLNPVMPFHFGGNYGETAHA"
     gene            complement(390..665)
                     /locus_tag="PXE44_001687"
     CDS             complement(390..665)
                     /locus_tag="PXE44_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5589186.1"
                     /translation="MIKSFKHKGLKQYFEKGITKGIQLNHQRKIDGILDLIDSAESID
                     EFMPFYQCHELKGNRKGIYSMTVNGNWRITFEFVNGDAYILNYEDYH"
     gene            957..1187
                     /locus_tag="PXE44_001688"
     CDS             957..1187
                     /locus_tag="PXE44_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589187.1"
                     /translation="MNAQATLNNHKDYILCGRKEKRTSDFINVFEVFENEATQEFVIE
                     RAMFRNGKLIDWNQSDKMNAEQAQQLWQAYIH"
     gene            1569..1805
                     /locus_tag="PXE44_001689"
     CDS             1569..1805
                     /locus_tag="PXE44_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589188.1"
                     /translation="MSNQEFVKLGSVEPSTIDELQKEDIRQAILKSVSITPYYVGLEI
                     AVCQAISAINKFSDGCITEELLDTKNGDKYRKNN"
     gene            2112..2187
                     /locus_tag="PXE44_001690"
     tRNA            2112..2187
                     /locus_tag="PXE44_001690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2145..2147,aa:Arg,seq:tct)
     gene            2374..2880
                     /locus_tag="PXE44_001691"
     CDS             2374..2880
                     /locus_tag="PXE44_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589189.1"
                     /translation="MKTKYKIIIERAKQQGLTHIELTQKHCLYLATSVGKLCYGVANN
                     AEQHIKLAAGEIFTLLTIINHQIQFDEDLELHTWKASGTTPPEQRAIRMLHHTSQFAW
                     FAPWLVDSNEKEQNQNTIRINLFNKCSGILIELNGILKEYELTLDECLDEFLKNKHCL
                     NEFSIHKE"
     gene            2884..3243
                     /locus_tag="PXE44_001692"
     CDS             2884..3243
                     /locus_tag="PXE44_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589190.1"
                     /translation="MTTFSIYTSVWHDSSTKGFRHNIKHKRHDCWRADIRIKQKINDV
                     WVQISRIRKRFKCPEQAKSFVEQWKQIDSPAFMQHQINRQALREELSNLEQRKQKQDG
                     ILEVIEYRISQIKELLK"
     gene            3240..3719
                     /locus_tag="PXE44_001693"
     CDS             3240..3719
                     /locus_tag="PXE44_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589191.1"
                     /translation="MKRNKVESYTIRLLKNNHTKRYFGEVILNGIKEISTAEMMSEIN
                     AIKAVNKRIEALNKTKAVNLPNYPEIRGHQPIMYNYNFEQIEEDPEQVKPKPSKPKRK
                     PFIPYGLNGYFVDSNGNIRLHLDRRASAETITLETDFFNVLAEMVKRTQEANNAKHA"
     gene            3853..4065
                     /locus_tag="PXE44_001694"
     CDS             3853..4065
                     /locus_tag="PXE44_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589192.1"
                     /translation="MIGYEDYTDASLQPEEDPSEEAFILAEENIIELLENNGFETIAT
                     ILAGSDGWRNAINAYLAEQNKHRNEF"
     gene            4078..5028
                     /locus_tag="PXE44_001695"
     CDS             4078..5028
                     /locus_tag="PXE44_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589193.1"
                     /translation="MTTANQQFELILKTESRVVSTNLQTFEEQANQYLATLTTTFETD
                     DDFAKAKEEVKELESIEKKIRDAIKQTQTGEIAELVLTAEQIAERFRQERLTRDKLVK
                     SKETEIKKQIADKAIEEITDTRNKLVKVSDISLALEITMPKHGIANRIAEAQKNKRTI
                     DSLTKAVNAEKALIISEMSVEIGRLTERLEQLAAKSAYLFPDAVKLIATQDDLAPIIQ
                     QRLAEEAEREAAIKAKAEADKVQAEAKAITDKMERKQAVENPQNVAKMNIEEPAQTDG
                     PLGDFIITIRLNQTIQTNAVSIARELKAKFGDSVSLNKAK"
     gene            5069..5863
                     /gene="bet"
                     /locus_tag="PXE44_001696"
     CDS             5069..5863
                     /gene="bet"
                     /locus_tag="PXE44_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDL5589194.1"
                     /translation="MTTALQTLTNKLAERFEMGDGSGLVETLKSSAFAGATVSDAQMI
                     ALLVIANQYQLNPWTKEIYAFPGGNGGLTPIVGVDGWVRIINREPQYDGMEFHFTDDY
                     SACTCTIYRKDRSKPIVVTEFMGECKKSSPAWNSHPKRMLRHKAMIQCARLAFGFTGI
                     YDQDEAERIAENEKPPKNITPQNNVVETTAVELISEEQLSQIRQLMQVTGTEEAKILA
                     YIGVQALNQIPKSQAEAVIKKLNLTLDKQNAEKADNGESVGEEIPL"
     gene            5860..6495
                     /locus_tag="PXE44_001697"
     CDS             5860..6495
                     /locus_tag="PXE44_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL5589195.1"
                     /translation="MTGDLIILDCEQGSEEWLNARLGIPTATGIENIVTPSGKKSSAW
                     NSYLAELVAESIEGAKDGFKSSDMARGNELEPLARMAYEFETDTEVVQVGGVYLNAEK
                     QLMVSPDGLIPNLKKGLEIKCPKMKTHIKYLLEGGVPNEYLIQVQSALWVTGYETWDF
                     VSYCPEYQKQPLYIYTAERDPVLMKAFDQYIPQFIKSLYALKGVTAPKGAF"
     gene            6508..6684
                     /locus_tag="PXE44_001698"
     CDS             6508..6684
                     /locus_tag="PXE44_001698"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589196.1"
                     /translation="MTTQKKYELLQNDTINHNGRTLYRIKAICHFRRTAEYSARATHA
                     TAAECYGGIACTST"
     gene            6705..7157
                     /locus_tag="PXE44_001699"
     CDS             6705..7157
                     /locus_tag="PXE44_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL5589197.1"
                     /translation="MKPFDLNKALAGEPVKLRNNDKAFVKYLISDDYIRDNKDHQVQG
                     YTVDEENVFLSEVSWAVSGSHFNDGTIAQYDIVGMWEEPRPAVTLTLPCPLKEPQENM
                     WFIDNDFTIVKSMFANAPFVKKFLPQGRCFASEEDAQEWLDAMRNSRR"
     gene            7235..7762
                     /locus_tag="PXE44_001700"
     CDS             7235..7762
                     /locus_tag="PXE44_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL5589198.1"
                     /translation="MNEEKYFSVDVSDETHVIRLHETLEQAKQSCLNGATEAYEFAGD
                     MDDHESYESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERASELFGEAEQVIEPPTLLE
                     NNGWISIEDKLPPIETDVLGLCDISGMQLILIVSRELADNEWYFLSVNQYGLDDDVIA
                     VTHWQPLPEPPKEEK"
     gene            7759..7962
                     /locus_tag="PXE44_001701"
     CDS             7759..7962
                     /locus_tag="PXE44_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589199.1"
                     /translation="MTDTERLDFIAQRKLDVRYSDLVDEFIIASYTKPHEQYFHWARN
                     ADLRTAIDEAMKEMRGLNNATTN"
     gene            7946..8410
                     /locus_tag="PXE44_001702"
     CDS             7946..8410
                     /locus_tag="PXE44_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5589200.1"
                     /translation="MQQLIKNIEQWAEDRNLINGSTPQKQMLKLMEEFGELCGGIARN
                     NPEMIKDAIGDLIVVCIIKAKQEKQPIYPVQFENHRLNGYRFDISVAVHCLYDIERIK
                     DRGKYSRFFGILHHLALINRFTLNDCLAHAYDQIKDRKGEMQAGIWVKEDDL"
     gene            8504..8674
                     /locus_tag="PXE44_001703"
                     /pseudo
     CDS             8504..8674
                     /locus_tag="PXE44_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253375.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8910..9401
                     /locus_tag="PXE44_001704"
     CDS             8910..9401
                     /locus_tag="PXE44_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5589201.1"
                     /translation="MQTKPILDACCGSRMFHFDKQNPLVLFADNRQIETTMKDRDKIR
                     HLEIKPDVLHDFTNMPYENESFWHIVFDPPHLVQGGENSWLVKKYGKLPCNWREVLKK
                     GFDECMRVLKPNGTLIFKWNETQIPVSEILKVIDCQPIYGHKSGRLNQTHWLAFVKIE
                     QSL"
     gene            9428..9685
                     /locus_tag="PXE44_001705"
     CDS             9428..9685
                     /locus_tag="PXE44_001705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589202.1"
                     /translation="MEQIKLSEKAERALILSQLNENSAMIIDLDDVALILNCGYDYVQ
                     SKISKKPGFPEPVQGINGKSVLFKSGDVIRWVNRQARTRKN"
     gene            complement(9682..10671)
                     /locus_tag="PXE44_001706"
     CDS             complement(9682..10671)
                     /locus_tag="PXE44_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5589203.1"
                     /translation="MASIRKRGSKYQVEISKKGVRKSATFRTKAEAQAWAYEEERKIE
                     IISKGGVVDILFAEALERYQNEVSINKRTWRREIGRINNILKEPIANRYVQDIKRDDI
                     EDWIEARLKRGLKGSSVERDLSILSNVFTYCLKRWGYISENPMIGVIHPNKVPPRKQR
                     YSQEDIDKILEVSKYREDIKRSNARLAAAMLFAIETAMRAGEISGLTWDNVNFEKRIA
                     HLPMTKNGSSRDVPLSSKAIEILKRLETIKKDNSVFQLPQGNGLSARFFELKEKAGLS
                     YLNFHDTRREALSRLAKKVDVMTLAKISGHKDVKILLNTYYAPNMEEVAHLLD"
BASE COUNT         3690 a         2214 c         2139 g         2835 t
ORIGIN      
        1 atattccttg aataaaccgc cctcgtggcg gttttctttt cctaagaaca ataaaatcct
       61 ccgaagaggg cattattaag cgtgagcagt ttctccataa tttccaccaa aatgaaaagg
      121 cataacagga tttaaccgct gctgctttaa ctcccataaa tcatatttag tttgtaagtt
      181 tagccataac ttagccgtac taattcctgc ctcttctaaa cttaacgcta aatttgcagt
      241 cattggtgtt ttgccgtgta atactcttga taaagtttca cgagaaaacc caagatgatc
      301 tgctaattct ttaattttga tgttatttgg ttcaataaat ccatctaata aaatttgccc
      361 cggatgtgct ggtttacgca taactccccc taatgataat cttcataatt caaaatataa
      421 gcatcgccat ttacaaattc aaatgtaatc cgccagttgc cattaaccgt catagaataa
      481 atgccttttc gatttccttt taactcgtgg cattggtaaa aaggcataaa ttcatcaata
      541 ctttctgctg aatcaatcaa atctaaaata ccatcgattt tacgttgatg attaagctga
      601 ataccttttg ttattccttt ttcaaaatat tgttttaagc ctttatgctt gaagctctta
      661 atcataataa cacctagcaa acacaatgtg atacaaatat atcacgcaca ataaaaaaga
      721 caatcttttt taagtgcttt ttctcctaat tgtctttctc ttcttatgtt aagtgtttaa
      781 aaaacaagca atcaaacaac ctttctaaaa ataatttctc tttaaaatca attatttaat
      841 cacgaattga aatttgtttg gctaaaataa ttacaaattg aattgactta atgatttcaa
      901 tttgtgataa taaacccatc aaaacaaaca acgccacaga caaagaggaa accaaaatga
      961 acgcacaagc aacgctaaat aaccacaaag actacatttt atgcggacgc aaagaaaaac
     1021 gcaccagtga tttcatcaat gtgtttgagg tctttgaaaa tgaagccacg caagagtttg
     1081 tgattgaaag agcaatgttc agaaatggca agttaatcga ctggaaccaa agcgacaaaa
     1141 tgaacgccga gcaagctcaa caactttggc aagcctacat tcactaagaa tttttatcaa
     1201 agcccttcac ggagggcttg aataaaggtt ctaaacctcg ccgaaaggca ttgttcttta
     1261 aaaattaaac caaaaacaca tcacgacaag cggattactt agcaatgtaa tgacattgga
     1321 gagacagaac caatctttgc ataaagcggt aaaacgtatg cggtaaatga acttataggc
     1381 aagttgtgaa actctctaag ctactcggac tgaaagaata gtacggtagt tgtggcgaat
     1441 ttactggtga tgtgcgaact agcggaaagg ttaagcactg gcagtgaatc gggaagcaca
     1501 aacgctggtt caattccaaa gcatatttga gaaagtgtgc tttggaatat taataacagg
     1561 agaacaaaat gagtaatcag gaatttgtta aattaggcag cgttgaacct tcaacgattg
     1621 atgaattaca gaaagaagat attaggcagg caatattaaa atccgtctct ataacgccat
     1681 attatgtggg gctggaaata gcggtttgcc aagcaatatc agccatcaat aaatttagtg
     1741 atggctgtat tacagaagag cttttagata cgaaaaatgg tgataaatac cgaaaaaata
     1801 actaatcaat aacaccaaag tgatttcatt ttttcggcat tagccctaga tgttgcccaa
     1861 gtattatatt cctcgaagtt gtggcaaaca agaggacttg gcccggtggc tcacaagtat
     1921 aaaaaagagc caccatctct aacagctctc aacagagagt gagacaaata accccgtttt
     1981 agttttaatg ttttctaaag tcggcgttag gcagcgaaac gaatgtacta gagagttgtt
     2041 agagatggtt agtccatcac attccttaga ttgaagtaag ccctgccgtt ggggttaagc
     2101 aacggtactt tgaccgcata gttcagtgga tagagcagct gccttctaag cagtgggtcg
     2161 agagttcgaa tctctctgcg gtcgccatcc aaaaccgctt tacgcacatc atcgatgacg
     2221 agcctacgaa gtggaaacct accgtgtaga gcggttttga atggctaagt tgtgtaactg
     2281 ctacaactta aaaagagcgg ttgataataa acataatgac tccgtttccc cgcctcgtgc
     2341 ggggcttttt ttatccacat aaaaggattc aaaatgaaaa ctaaatacaa aattattatt
     2401 gagcgagcaa aacaacaggg tttgacccat attgagctta ctcaaaaaca ttgtctgtat
     2461 ctcgctacat cagtagggaa actgtgctac ggggtagcta ataatgcaga acaacatatt
     2521 aaacttgctg ctggagagat ttttacacta ctaacgatca ttaaccatca aatccagttc
     2581 gatgaagact tagagcttca cacgtggaaa gctagtggaa caaccccacc cgagcaaaga
     2641 gcgatacgta tgttacatca tacctcgcaa tttgcttggt ttgctccttg gttagtggac
     2701 agcaatgaaa aagaacaaaa ccaaaataca atcaggatta atttatttaa caaatgctct
     2761 gggattctga ttgaactaaa tggcattctt aaagaatacg agctaactct tgatgagtgc
     2821 ttagatgaat tcttgaaaaa taagcattgt ttgaatgaat ttagtattca caaggagtaa
     2881 aaaatgacga cgtttagtat ttataccagt gtatggcacg acagctccac gaaaggcttt
     2941 cgccacaaca ttaagcataa acgccacgat tgctggagag cggatatccg cataaagcaa
     3001 aaaatcaatg atgtgtgggt gcagatttca aggatacgca aacgcttcaa gtgccctgaa
     3061 caggcaaaat cctttgtaga acaatggaaa caaattgatt cacctgcatt tatgcaacac
     3121 caaatcaacc gccaagcatt aagagaagaa ttatcaaacc ttgagcaacg caaacagaag
     3181 caagacggaa tacttgaggt gattgagtat cgtattagcc aaatcaagga gttgctgaaa
     3241 tgaaacgcaa taaagtagaa agctatacta tccgcttatt aaaaaataat catacaaaac
     3301 gctacttcgg agaagttata ttaaacggca taaaagaaat cagcacggca gagatgatgt
     3361 ctgaaattaa cgctattaag gcagtaaata agcgcattga agctctgaat aaaaccaaag
     3421 ccgttaattt gcctaattat cctgaaatca ggggtcatca gcctattatg tacaattaca
     3481 attttgaaca aattgaggaa gatcctgaac aggttaagcc taaaccatca aaaccaaaac
     3541 gcaagccatt cataccttat gggctaaacg gctactttgt ggatagcaac ggtaatattc
     3601 gcttacatct agacagacga gcaagtgccg aaacaattac ccttgaaact gatttcttca
     3661 acgttcttgc cgaaatggta aaacgtacac aagaggcaaa caatgctaaa catgcttaga
     3721 caatggatta aatacggctt atacgccctg ctttttatgg ttgccatcac cctcctaatg
     3781 caatgaagag cttatccctg aaagcacctg caagcctgaa acttgcgaat ttaaagtaac
     3841 ggagaaagtg tgatgatagg atacgaagac tacacagatg cttcacttca accagaagaa
     3901 gaccctagcg aagaggcttt catcttggct gaagaaaaca tcatcgagct attagaaaac
     3961 aacggctttg aaaccattgc caccatccta gccggttctg atggctggcg taacgcaatc
     4021 aacgcctacc tagccgaaca gaacaaacac agaaatgaat tttagaggaa taagaaaatg
     4081 acaactgcaa atcaacaatt tgaacttatt ttaaaaaccg aaagccgagt ggtttctacc
     4141 aacttgcaaa cttttgaaga gcaagcaaat caataccttg ccacgctcac caccaccttt
     4201 gaaaccgatg atgattttgc aaaagcaaaa gaagaagtga aggagcttga aagcattgag
     4261 aaaaaaattc gtgacgcaat taaacaaacg caaactggcg agattgcgga attggtatta
     4321 acagcagaac aaatcgctga acgtttccgc caagagcgat taacccgaga caagctcgtt
     4381 aagagcaaag aaacggaaat caaaaaacag attgccgata aagccattga agaaatcact
     4441 gataccagaa acaagctggt taaagtaagt gatatttccc ttgccttaga aatcacaatg
     4501 ccaaaacacg gcattgctaa tcgtattgcc gaggcacaga aaaataaacg cacgattgac
     4561 agcttaacca aagccgtgaa cgccgaaaaa gcgctgatta tcagcgaaat gtcggtagaa
     4621 atcgggcgct tgactgagcg attagaacag cttgctgcaa aatcagctta tctttttcct
     4681 gatgcagtga aattgatcgc aacccaagac gacctcgccc caattattca acaacgcctt
     4741 gccgaagaag ccgagcgtga ggcggcgatt aaagccaaag cagaagccga caaagtacaa
     4801 gccgaagcca aagcgattac tgataaaatg gagcgcaaac aagcggtcga aaatccgcaa
     4861 aatgtggcaa aaatgaacat cgaagaaccg gcacaaaccg atgggccatt aggcgatttt
     4921 atcattacta ttcgcttaaa ccaaacaatc caaaccaacg cagtcagtat tgctcgtgag
     4981 ctgaaagcga aatttgggga tagcgtttcg ctcaataagg ctaaatgatt tgataccgca
     5041 caaattcaac ctaaataagg atcatattat gacaactgca ttacaaacac taaccaacaa
     5101 acttgccgag cgttttgaaa tgggagatgg ctcaggatta gttgagacac ttaaatcgtc
     5161 tgctttcgca ggggcaaccg taagcgatgc tcaaatgatt gcactattgg ttattgctaa
     5221 ccaataccaa ttaaaccctt ggacaaaaga aatttatgca ttccccggtg ggaacggtgg
     5281 attaacgcct attgtcggcg ttgatggttg ggttcgcatt attaaccgtg agcctcaata
     5341 tgatggaatg gaatttcatt ttaccgatga ctattccgcc tgtacttgta ctatctaccg
     5401 taaagataga agtaaaccga ttgtggtgac ggaatttatg ggggaatgta aaaagagctc
     5461 tccagcttgg aactctcacc caaaaagaat gttacgccat aaagcaatga tccaatgtgc
     5521 aagattagcc tttggcttta cgggtattta cgaccaagac gaagcagagc gtattgcgga
     5581 aaatgaaaaa ccacctaaaa acatcacacc acaaaataac gtggtagaaa ccactgccgt
     5641 tgaactcatt tcggaagaac aactgtcgca aatccggcag ttaatgcaag taaccggtac
     5701 ggaggaggcg aaaattctcg cctatattgg cgttcaagca ctgaaccaaa tccctaaatc
     5761 gcaggcagag gcagtgatta aaaagctcaa tcttacgcta gacaagcaaa acgctgaaaa
     5821 ggcggataat ggcgaaagtg tcggtgagga aatcccacta tgacaggcga tttaatcatt
     5881 ttagattgcg aacaaggatc agaagaatgg ctcaatgcca gattgggcat tcccaccgcc
     5941 acaggcattg aaaatatcgt tactccaagc ggtaaaaaat cgtccgcttg gaatagctat
     6001 cttgccgaac tggttgccga aagcattgag ggagcaaaag acggttttaa gtcgtctgat
     6061 atggcacgag gcaacgagct tgaaccgctt gcacgaatgg cttacgagtt tgaaaccgac
     6121 accgaagtcg tgcaagtcgg cggtgtgtac cttaacgccg aaaaacagtt aatggtaagt
     6181 cctgacggct taattcccaa tctaaaaaaa gggttggaaa tcaaatgccc gaaaatgaaa
     6241 acccacatca aatacctgct agaaggcggt gtgccgaatg aatatcttat tcaagtgcaa
     6301 tctgcccttt gggtaacagg ctacgaaacg tgggactttg tcagctactg ccccgaatac
     6361 caaaaacaac cgctttacat ctacacagcc gaacgtgatc cggtattgat gaaagccttt
     6421 gatcaatata tcccgcaatt tatcaaatcc ctttatgcat tgaagggggt aaccgccccg
     6481 aaaggggctt tttaataggt agccaatatg acaacccaaa agaaatacga actactccaa
     6541 aacgacacaa tcaaccacaa cggcagaaca ctctaccgca tcaaagccat atgccacttt
     6601 aggcggacgg ctgaatattc cgcaagagcc actcacgcca cagccgctga atgctatggt
     6661 ggaattgcct gtacatcaac ataaccacaa acacggagaa taaaatgaaa ccatttgatt
     6721 taaataaagc attggcaggt gagccagtaa aactaagaaa taatgataag gcatttgtaa
     6781 aatacctaat tagtgatgac tatattagag ataataaaga ccaccaagta caagggtata
     6841 cagttgatga agaaaatgta tttttgtcag aagtatcgtg ggctgttagc ggatctcatt
     6901 tcaatgatgg aacaatagct caatatgata ttgttggtat gtgggaagaa ccaagaccag
     6961 cagtaacatt gacgttacct tgcccattaa aagaacctca agaaaatatg tggtttattg
     7021 acaatgattt taccatagta aaatctatgt tcgcaaatgc cccctttgta aaaaaattct
     7081 tgccacaagg gcgatgtttc gcttcagaag aagacgctca agaatggtta gatgcaatgc
     7141 gaaatagtcg taggtaaatc ggcagcacta acagctgaat gctgctgttg aatttcttgt
     7201 atatccacat aaacacggaa cgcggagaaa gaaaatgaat gaagaaaaat atttttcggt
     7261 cgatgtctcg gatgagacac atgtaataag gttgcacgaa acattagagc aagcaaaaca
     7321 aagctgttta aatggtgcta ctgaagccta tgagtttgcg ggtgacatgg atgatcacga
     7381 aagctatgaa agctatgaag cctatgattt accctatgca gtttatggtg ttgttttagg
     7441 tagagcaaaa tcagatattc gtccactaac agacgaagaa agagcatcgg agttatttgg
     7501 ggaagctgaa caagttattg aaccaccaac acttttagaa aacaacggct ggatttccat
     7561 tgaggacaaa ttaccaccta ttgaaacgga tgtgctaggg ttatgcgata tttcaggaat
     7621 gcagttaatt ttaatagttt caagagaact tgccgataac gagtggtatt ttcttagtgt
     7681 aaatcaatat gggcttgatg acgatgtgat tgcagtaacc cactggcaac cacttcccga
     7741 accaccaaag gaggaaaaat gaccgacacc gaacgcttag actttatcgc ccaacgcaaa
     7801 ttagatgtcc gatattctga tttggtggac gaatttatta ttgctagcta taccaaaccg
     7861 cacgagcagt attttcattg ggcaagaaat gccgatttac gcacggcgat tgatgaagca
     7921 atgaaagaaa tgagaggact aaacaatgca acaactaatt aaaaacatcg aacaatgggc
     7981 ggaagaccgc aatttaatta acggctcaac cccacaaaag caaatgctca aactgatgga
     8041 agagtttggc gaactctgcg gaggcattgc ccgcaataat cctgaaatga ttaaagatgc
     8101 gattggggat ttgattgttg tctgtattat taaagcaaaa caagaaaaac aacctattta
     8161 ccctgtacaa tttgaaaatc atagattaaa tggttacaga tttgatattt cggtcgctgt
     8221 tcattgtcta tatgatattg aacgcattaa agacagaggt aaatatagcc gtttttttgg
     8281 catattacac catttagcct taataaatag atttacgctt aatgactgcc tcgctcacgc
     8341 ctacgaccaa atcaaagatc gcaagggcga aatgcaagct gggatttggg ttaaagaaga
     8401 cgatttatag tacctaagct ataaaaaccg aaacatcttg cagaaaagca ttccgccact
     8461 ctataagagt ttcggtttct tcttcgttgg cgttgattat gtttacacgc acgctaccga
     8521 gtatcaaacc acgcttggcg taatgaagcg gatacttgaa ccgatgttag ccgacttcga
     8581 ggtcgatccg tttgaagagg ctcactacca caaagcccta actaccttaa ggcaatacca
     8641 gccgaaaggc ttaggcggct tagtccgaat ttaatttgca aaaaaatcac acaatccgac
     8701 cgcttgtgaa acaacaagcg gcggatttcc atacccaaat tttgaggaca agccgatgaa
     8761 atacgcaaaa atcattctat ttttaaccgc ctttgcagtt gctgctgacc atctagagtt
     8821 acgcaacgac tgcgatggca aaatttgtat ggctcaacgc taattaaccc aaccgcttgt
     8881 tccgcaagcg gttatttttt ggaggaaaaa tgcaaacaaa accgatatta gatgcgtgtt
     8941 gtggttcccg tatgtttcat tttgacaagc aaaatccact cgtcctgttt gctgataatc
     9001 gacaaataga aaccacaatg aaagaccgag acaaaatccg ccatttagaa atcaaacctg
     9061 atgttttaca cgatttcacg aatatgccat acgaaaacga gagtttttgg cacattgttt
     9121 ttgatccgcc acatttagtt caaggtggag aaaatagctg gcttgtaaag aaatacggca
     9181 aattaccttg caactggcga gaagtcttga agaaaggctt tgacgaatgt atgcgagtgc
     9241 taaaaccaaa cggcacgctt attttcaagt ggaatgaaac acaaatcccc gtttcagaaa
     9301 ttttaaaagt tattgattgt cagccgattt atggacacaa aagcggacgc ttaaaccaaa
     9361 cacattggct ggcgtttgtg aaaattgagc aatccctcta aatgaggggt ttatttttgg
     9421 agagaatatg gaacaaatta aactttcaga aaaagcggag agagccttaa ttctttctca
     9481 gctcaacgaa aattcagcaa tgattattga tttagatgat gttgccctga ttttgaattg
     9541 cggatatgac tatgtgcaat ccaagatcag taaaaagccc ggtttcccag agccagtgca
     9601 aggcattaac gggaaatcag ttcttttcaa aagtggcgat gttatccgtt gggtcaaccg
     9661 tcaagcacga acacgcaaaa attaatccag caaatgagcg acttcttcca tatttggggc
     9721 gtagtaagtg ttaagcaaaa tcttcacatc tttatgccct gaaattttgg ctaaggtcat
     9781 cacatcgact ttttttgcca agcgtgaaag agcctctctc ctcgtgtcgt gaaaattcaa
     9841 ataagaaagc ccagcctttt cttttaactc aaagaatcta gcacttaaac cattcccttg
     9901 tggaagctga aaaacggaat tatccttttt gatggtttcc agtcttttca agatctcgat
     9961 tgcctttgat gataacggaa cgtctcttga tgaaccgttt tttgtcatag gcaaatgagc
    10021 tattcttttc tcaaaattca cattatccca cgttaaaccc gaaatctcac cggcacgcat
    10081 tgccgtttca atcgcaaata acatagccgc agccaatcga gcattagagc gtttaatatc
    10141 ttctcggtat ttgctgactt ctaagatttt atcaatatct tcctgggaat aacgctgttt
    10201 tcttggtggc actttgtttg ggtgaataac accgatcatc ggattttcac taatatagcc
    10261 ccagcgtttt aagcagtagg taaatacatt gcttaaaata gacaaatccc tctcaacact
    10321 tgagcctttt aaccctctct tcaatctagc ttcgatccaa tcttcaatat catctcgctt
    10381 aatatcttgt acataacgat tcgcaatagg ctctttcaat atgttattga ttcgacctat
    10441 ctctcgtcgc cacgtcctct tattaatcga tacttcattt tgataccgct ctaacgcttc
    10501 cgcaaataaa atatcaacta ccccaccttt cgagatgatc tctattttac gctcttcttc
    10561 gtaagcccat gcctgtgcct ctgcttttgt gcggaaggta gctgattttc tcaccccttt
    10621 cttcgagatt tcgacttgat atttactgcc ccgcttgcga atgctcgcca tatttttacc
    10681 tgccttttgt cgtaaatttg tcgtaattta taccatacaa taaccgacaa taacagataa
    10741 taaccgattt tatgaaatca tcttaaggca aagaagagag gaaaaactta agataaccta
    10801 ttgaaataac tataataaac gataataacc gacaaaaaac ggaaataaaa aaaagcccct
    10861 aatcaaaaaa gcgaaaag
//
LOCUS       DANLZC010000087        10862 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.087, whole genome shotgun sequence.
ACCESSION   DANLZC010000087 DANLZC010000000
VERSION     DANLZC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10862
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.087"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            31..414
                     /locus_tag="PXE44_001707"
     CDS             31..414
                     /locus_tag="PXE44_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5589204.1"
                     /translation="MVFTSYSIKSKKLCPTILALSVSSILSSQATYAEETTAELSEVN
                     VVAKEDVKASIEKKNKEIIQQELIQNNRDLVRYSPDVGIVNQGRHQKGFAIRGVEDNR
                     VGISIDGVSLFLIQKKIPFINAMVI"
     gene            381..2459
                     /locus_tag="PXE44_001708"
                     /pseudo
     CDS             381..2459
                     /locus_tag="PXE44_001708"
                     /inference="COORDINATES: protein motif:HMM:TIGR01786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            2514..3020
                     /gene="hutX"
                     /locus_tag="PXE44_001709"
     CDS             2514..3020
                     /gene="hutX"
                     /locus_tag="PXE44_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDL5589205.1"
                     /translation="MISIEQQTALRNELAENPGQILEMLAAKYQCTLEEVMLNLPAEM
                     LKVTEGERFAEVLQQIHEWDDAITLISHTEDTIIEFVGKLPSGSISRGFYNFEHKEGG
                     GLQGHLRYENCAKIYLLDRPFMGKRTVSLIFINKKGNAMFKIFVGREKIGGALKEHQI
                     KALYQLIG"
     gene            complement(3100..4317)
                     /locus_tag="PXE44_001710"
     CDS             complement(3100..4317)
                     /locus_tag="PXE44_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter"
                     /protein_id="HDL5589206.1"
                     /translation="MKNKILGSALMIAGTTIGAGMLAMPLTSAGMGFGATALLLVGLW
                     ALLAYTGLLFMEVYQTAPQKDVGVASLAEQYFGLIGRVLATFSLLVLLYALLAAYITG
                     GGSLLAGVMPEMADADMKLKISILIFTAVLGTFVVVGVKSVDGLTRVLFLGKIVAFIA
                     VLLMMLPKAKLENLTAIPLDNLLIISAIPIFFTAFGFHVIMGTINSYLDADIGKIRKS
                     IYIGTAIPLVAYLLWQLATHGVLSQNEFTTILTQDPTLNGLVKATSQITGSTILGEMV
                     RLFSVLALITSFLGVSMGIFEGVGDLLKRLNLPTNRLWLTIATFTPPILFALFYPNGF
                     IKALGYAGLLFAFYGMILPIGLAWKARRQYPNLPYRVIGGNLALVVALIAGIIIMVIP
                     FLIQAGYLPQAVG"
     gene            4478..8011
                     /gene="mfd"
                     /locus_tag="PXE44_001711"
     CDS             4478..8011
                     /gene="mfd"
                     /locus_tag="PXE44_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL5589207.1"
                     /translation="MSLISLNLPKSDGNFQDHQTLGNLVGHSDTLAISQAARAFYGLT
                     VVVTPDTRTALRLEKALPQFAGLPVQLFPDWETLPYDNFSPHQDIISARLSALFELQQ
                     GRQQILLLPINTLMQKVCPPNYLANNVLLIKKGDRFSIQKLRLQLENAGYRAVEQVLE
                     YGEYAVRGSILDLFPMGAEEPFRLDFFDDEIDSIRTFDVDNQRTKTEIAEINLLPAHE
                     FPTDSNGIEHFRAKFREKFGEIRREPEHIYQQVSKGILNAGIEYWQPLFFEEMASLFD
                     YFPAQTLFITFADIQQKAEQFHQDTQNRYESHRVDPMRPLLPPSDLWFAIDEVNRWLK
                     GYPRLTITSEKIRKSAAKMNANVANLPDIAIQSSAKEPFSAFQQFRQKFDGNILFSVE
                     SEGRRETLLDLLAPLGVKPKQVNSLDEAQGLPFSLIISPLDQGFVVETGGHGTRPYDD
                     ELNTNGENDNNYVGAGPVPARNLAIICETDLLGERVQTRREKNRKAVNPDTLIRNLAE
                     LKIGQAVVHLENGVGRYGGLTVLDAGGIKAEYLVLHYANDAKLYVPVASLHLISRYIG
                     GADETAPLHKLGSEAWAKTRQKAAEKIRDVAAELLDVYAKRESQTGFAFAYDKAEFDQ
                     FSATFPYEETDDQKIAINAVISDMCQAKAMDRLVCGDVGFGKTEVAIRATFLAVMNHK
                     QVVILAPTTLLAQQHYENFKDRFANYPVNVEVLSRFKTAKEQKTILQNVAEGKVDILV
                     GTHKLLQDDVAFRDLGLLVIDEEHRFGVRQKEKIKQLRANVDILTLTATPIPRTLNMA
                     LNGMRDLSIIASPPARRLLIKTFVRQSDEMVIREAILREILRGGQVYYLHNDVATIEN
                     CAEKLAELVPEARIVIGHGQMRERELERVMSDFYHQRFNLLVCSTIIETGIDVPTANT
                     IIIERADKFGLAQLHQLRGRVGRSHHQAYAYMLTPHPKTLTKDAEQRLEAMSTIDNLG
                     AGFALATHDLEIRGAGELLGSEQSGQIESIGFSLYMDLLENAVKALQEGREPSLDEIT
                     QHQAEIELRVPALLPDDYLPDVNMRLSFYKRIASSENLQSLNDLKVELIDRFGLLPEA
                     TKNLFAITQLRLSAQTLGIKKIDAGIHGGYLEFKPTAQPDPMKFIALIQKQPAVYSFE
                     GAVKFKFRLALEDNRKRLEFVENLLREVVGG"
     gene            8174..8461
                     /locus_tag="PXE44_001712"
     CDS             8174..8461
                     /locus_tag="PXE44_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5589208.1"
                     /translation="MEEINGFEWDSEKALRNQQKHGVSFIEAVSVFYDNNAMLIPDPD
                     HSSREERFLLLGRSNKDNLLVVVHCERNTNIRIISARLATKRESGQYKGVH"
     gene            8462..8674
                     /locus_tag="PXE44_001713"
     CDS             8462..8674
                     /locus_tag="PXE44_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL5589209.1"
                     /translation="MRDEYDFSNAVKNPYAKGLKSKSVVTIRIEDDVISYFKQLAEEV
                     NLPYQTLINLYLKDCVEQKRKPTISW"
     gene            8790..9050
                     /locus_tag="PXE44_001714"
     CDS             8790..9050
                     /locus_tag="PXE44_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL5589210.1"
                     /translation="MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLR
                     SRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQRDPFEVA"
     gene            9051..9440
                     /locus_tag="PXE44_001715"
     CDS             9051..9440
                     /locus_tag="PXE44_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL5589211.1"
                     /translation="MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYG
                     VAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKLRAEMEKNGKVMGVQDQMIASHA
                     LAEECILVSSDQAFKMVPDLKLENWLH"
     gene            9460..10698
                     /gene="pepT"
                     /locus_tag="PXE44_001716"
     CDS             9460..10698
                     /gene="pepT"
                     /locus_tag="PXE44_001716"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL5589212.1"
                     /translation="MKQSLLNRLLRYTQIHTTSDPKSQTVPSSQQQWDLLHLLKTEVE
                     AMGLSEIYLDPNGYLFASLPANIDKTLPVLGLIAHVDTSPDFNGENVKPQVIEAYQGG
                     NILLNGSGDYLSPTDFPNLNKLIGDTLVTTDGTSLLGADDKSGIAIILSAIEYLQANP
                     HIPHGKIRIGFTTDEEIGRGADHFDVNAFGADVAFTFDGGPLGELQYENFNADAATVT
                     FFGRSVHPGTAKGKMINALERACEFQHRLPPHLTPATSEGREGFIMLHGIEGGIDKAE
                     IHYILRSFERDELAAFGKMLNDTLAEMQQIYGEHCGKVEIVEQYRNMREVLDDYGYLV
                     EIAENAMREQGIEPNLEPIRGGTDGSRLSFMGLPCPNLFTGGENFHGRFEFISVDTME
                     KATKVVIDICRKFADYDFNK"
BASE COUNT         3263 a         2166 c         2480 g         2953 t
ORIGIN      
        1 agtatattaa aaatctatct agggagaatc atggtgttta cctcttattc aataaagtct
       61 aaaaaattgt gcccgactat attggcttta agcgttagta gcattttgtc ttcacaagct
      121 acctatgcgg aagaaacaac agcggaatta tctgaagtaa atgttgttgc taaagaagat
      181 gtgaaggctt ctatagaaaa gaaaaataaa gagataattc agcaagagtt gattcaaaat
      241 aatcgagatt tagttcgtta tagccctgat gttggcattg ttaaccaagg tagacatcaa
      301 aaaggatttg ctattcgtgg agtggaagac aaccgtgtag gaataagtat tgatggtgtc
      361 tcgcttttcc tgattcagaa gaaaattccc tttataaacg ctatggtaat ttaaatacat
      421 ctcgccaatc tatagatccg gaattagctc gtactattga agtatctaaa ggtgcagatt
      481 ctttcaatca aggtagtggt aatttaggcg gcggagttaa ttatcggacg cttgagcctt
      541 atgatattgt tcgtaacggt aataaatttg gtgcttttta tcgcacagga tatggtactc
      601 gtaataatga atggttgaat acattcggtg ttggatattt aggtgagaaa gctgaggcga
      661 tattggttta ttcaaatcgc catgggcatg aaatgaaaag tgcaggaggt tatacacttc
      721 ctgaagattc tctacaaact cgctctttac gcagtagtaa acaaactcct gatgattcta
      781 ctcataatca tcataattat ttagccaaat ttgcttatcg atttaatgat aatcaccgcg
      841 ttggtgtttc ttacagtggg caaaataata aaaattatat tatcgaagat tcagccgtta
      901 ctattacttc tttttggcgt gaagcggaag accgtagtaa acgtgataca gtaaatgttt
      961 tttatgagta tttcccagag tcaaaatgga tatctctagc gaagatagat gcagattatc
     1021 aagatactga aacatctgct tataactatg aaggtacacg cgctgaagat gcaacagact
     1081 ggagccctgc taaacaaaga aaaccaagtg acaataatat tcgtattttt aatactgaat
     1141 tgaaacgctt aaatttccgt ttagacagcc aagcattaga atttggtaaa tggactcatc
     1201 agctatcttt caaagcctct actgcagaga gagatttcga tgtattacat aaggactcgg
     1261 tttatttatc cggtgtttgg gtaaattctc ccgattctac gatgatgtac ccaattaaaa
     1321 cgaaacagca cactttttca ttgcacgata agattgattt aacgcatgat tggattgcaa
     1381 cgctaggcat tcgttatgat tgggcgaagt atgaacaaca agaactcaat ggtcttgttt
     1441 gtcgtaactg cgtaaaaact gacaatgcga aatttaatca actaacttgg gttaccggtt
     1501 tagaaaaaca tctgactgag gcatggaaag taggatataa cattgggaca ggcttccgta
     1561 ttccaaatgc atcagaaatg tattttgatt accgtgataa tgctgcagga gcttggatgt
     1621 ccaatccaaa cctaaaagcg gaacgtagtt taagccaaaa tttaagtttg caaggtagtg
     1681 gcaatgttgg gctgctctca gttaatttcc accatactaa atataaagat tttttatggg
     1741 agcaagaaac ctgggatgtt taccaagcat atggtagaga gttttggcgc ccggtacagc
     1801 aaatgcaaaa tattgattct gcgaaaatct acgggctaga agtgagtggt aaatggaatt
     1861 taaattcagc tatgccaata cctgaaggct ggaagctatt tggagccttc ggttacagca
     1921 aaggtagtat gtctaacggg gcagatttac tttctattca gccgattaaa gccgtagttg
     1981 gcttagatta tgaacaacct gaaggtaaat ggggagtatt ttctcgcttc acattcttgg
     2041 gggctaaaaa ggcgaaagat gcaaaatatt taaaaacatt accggaaaaa tgccttaaag
     2101 aggatagaac gccaaatccg tattaccctt attgggggga tgaatatgac attcgttgta
     2161 ctgaatattc tcacgagaca ggtttagata cctggaagca cttaaatgct aaagcctttg
     2221 ttgtggattt atacggtttc tataaaccaa ctgagaatat tacattacgt gccggtattt
     2281 ataatttgtt taaccgtaaa taccatactt gggatacttt acgtggttta aatacaacag
     2341 gtggaatcgt taattctgtt ggagtacgtg aaaatgcaac ttacggtggc tatcctggat
     2401 tacaacgcta ttatgaacct ggacgtaact tctcggctaa ttttgaatac cgtttctaat
     2461 ttacaaattt acttagagag ctaagaaact ctctttactt aaaggaacat ttaatgatat
     2521 caatcgaaca acaaaccgcc ttacgcaatg aattagccga aaatccgggg caaattttag
     2581 aaatgttagc ggcaaaatat caatgcacgc tggaagaggt aatgctgaat ttaccggctg
     2641 aaatgctaaa agtaaccgaa ggggaacgtt ttgccgaagt gctacagcaa atccacgaat
     2701 gggacgatgc gattacgctg atttcgcata cggaagatac cattattgag tttgtgggaa
     2761 agctcccaag cggttcaatt tctcgtggat tttacaattt cgaacataaa gagggcggag
     2821 gcttacaagg gcatctacgc tacgaaaatt gtgccaaaat ttatttgttg gatcgtcctt
     2881 ttatgggaaa acgtactgtt tcactgattt tcattaataa aaaaggcaac gcaatgttta
     2941 agatttttgt cggcagagag aaaattggcg gggcattaaa agagcatcaa attaaggcgc
     3001 tgtatcagtt gattggctaa tctgtaaaaa gtcatcgaaa aatgaccgct tgtaacttat
     3061 agaaagggct acgatattga atcgtagccc ttttcaatat tagcctaccg cttgcggtaa
     3121 atagccagcc tgaattaaaa acggaatgac cataatgata ataccggcaa ttaacgcaac
     3181 cactaatgcc aaattaccac caatcacacg gtaaggtaaa ttcggatatt ggcgacgtgc
     3241 tttccacgct aagccgatag gtaaaatcat accatagaac gcaaagagta aaccggcgta
     3301 acctaatgcc ttaataaagc cgttcggata gaataaagcg aacagaattg gcggtgtaaa
     3361 ggtggcgatg gtcagccata aacggttagt cggtagattt aagcgtttta acaaatcgcc
     3421 cacgccttca aaaattccca tcgaaacccc taaaaatgag gtaatcaatg caagcactga
     3481 gaataagcga accatttcgc ctaaaatcgt actaccggta atctggctag tggctttcac
     3541 caagccgttt aaggtcggat cttgtgtcaa aattgtggtg aattcatttt ggcttaatac
     3601 cccgtgtgta gcgagctgcc acaataaata agccactagc ggaattgccg tcccgatata
     3661 aatagattta cgaattttgc cgatatctgc gtctaaatag ctattaattg ttcccataat
     3721 aacgtggaaa ccgaacgcag tgaagaaaat gggtatagca gaaataatga gtaagttatc
     3781 taatggaatt gctgtcaagt tttccagctt cgctttaggc agcatcatca gcaacacggc
     3841 aataaaagca acaatttttc ccaaaaacag tactcgggtt aaaccatcaa ccgatttcac
     3901 gccgactacc acaaatgtac ctaataccgc agtgaagatt aaaatgctga ttttcagttt
     3961 catatcagca tctgccattt caggcattac accggctaac agtgaaccac caccggtaat
     4021 ataagccgca agtagggcat aaagtagtac gagcaaactg aaagtggcta atacacgccc
     4081 gattaagcca aagtattgct cggcaaggct tgccactcca acatcttttt gcggagcggt
     4141 ttgataaact tccataaata ataaaccggt gtaggccaat aaagcccata atccgactaa
     4201 gagtaatgcg gttgccccaa aacccattcc tgcagaggtg agtggcattg caagcattcc
     4261 tgcaccaatg gtagtgcctg caatcattaa tgcacttcct aaaattttat ttttcataat
     4321 atgtgtgagt aagagttaaa aaaggggaac attttatcag aattttttcg caatgcgatc
     4381 tagataacat aatttctccg cttattttgc acaatcagcc gtgctttagt aaactaagca
     4441 acaatttgca attttttatt taaaacagac cgcttgtatg tctttaattt cacttaatct
     4501 gccgaaatct gacggcaatt ttcaagatca tcaaaccctc ggtaaccttg tcgggcattc
     4561 cgataccctt gcgatcagcc aagctgcccg agccttttac ggcttgacgg tggttgtcac
     4621 acccgacacc cgcacggcgt tacgcttgga aaaagccttg cctcagtttg cagggctgcc
     4681 agtgcaactg ttccccgatt gggaaacctt gccttatgac aatttttcgc cgcaccaaga
     4741 cattatctcc gcccgacttt ctgccctgtt tgaactgcaa caagggcgac agcagatttt
     4801 gctgttgccg atcaacacct taatgcagaa ggtctgcccg ccgaattatc tcgctaacaa
     4861 tgtgctgttg attaaaaaag gcgaccgttt ttcgattcaa aaactgcggt tacaactgga
     4921 aaatgccggc tatcgggcgg tggaacaggt gttggaatat ggtgaatacg ccgtgcgtgg
     4981 ctcgatttta gacctattcc cgatgggagc agaagagccg tttcgcctcg attttttcga
     5041 tgatgagatc gacagtatcc gcacttttga tgtggataac caacgcacca aaacggaaat
     5101 tgccgaaatt aatttactgc cggcacacga gttcccgact gatagcaacg ggatcgagca
     5161 ttttagagcc aaattccgtg aaaaattcgg tgaaatccgc cgtgagccgg agcatattta
     5221 tcagcaagtc agcaaaggca ttttaaatgc agggattgag tattggcagc cgttgttttt
     5281 tgaggaaatg gcaagtctgt ttgactattt ccctgcccaa acgctgttta tcactttcgc
     5341 cgatattcag caaaaagctg aacaatttca ccaagatacc caaaaccgct atgaaagcca
     5401 ccgagtggat ccgatgcgac cgctactgcc gccgagtgat ctctggtttg cgattgatga
     5461 ggtaaatcgc tggctgaaag gctatccccg tttaacgata acttcggaga aaatccgtaa
     5521 atcagcagcg aaaatgaatg caaatgtggc taacttgccg gatattgcta ttcaatcctc
     5581 tgccaaagag ccgttttctg ctttccagca attccgccaa aaatttgacg gcaacattct
     5641 gttttcggtg gagagcgaag ggcgaagaga aaccttgctg gatttgcttg ctccgcttgg
     5701 ggtgaagccg aagcaggtca attcattaga tgaagcacaa ggtttgccat tttccctgat
     5761 tatttcaccg cttgatcaag ggtttgtggt ggaaacgggc gggcacggga cccgtcccta
     5821 cgatgatgaa ttaaatacaa atggcgaaaa tgataataac tacgtagggg cgggtcctgt
     5881 gcccgcccgc aaccttgcca tcatctgcga aaccgacctg ctgggcgaac gtgtgcaaac
     5941 acggcgtgag aaaaaccgca aagccgtcaa tcctgatacc ttaatccgta atttagcaga
     6001 actcaaaatc ggacaggcgg tggtgcattt ggaaaacggc gtggggcgtt acggtggctt
     6061 aaccgtgctg gacgctggtg ggatcaaagc cgaatatctc gtgctacatt atgctaacga
     6121 tgccaaactc tatgtgccgg ttgcctcgct ccatttaatc agccgttata tcggtggggc
     6181 ggatgaaact gcgccgctac ataaattagg cagtgaagcg tgggcgaaaa cccgccaaaa
     6241 agcagcggaa aaaatccgtg atgtggcggc agaattgctt gatgtttatg ccaaacgaga
     6301 aagccaaacc ggttttgcgt ttgcctacga taaagcggaa tttgatcagt ttagtgccac
     6361 tttcccttat gaggaaacgg acgatcagaa aattgccatc aatgccgtaa ttagcgatat
     6421 gtgccaagcc aaagcgatgg atagattggt gtgtggcgat gtcggcttcg gcaaaaccga
     6481 agtggccatt cgagcgactt tcttggcagt gatgaaccat aaacaggtgg ttattcttgc
     6541 cccaactacc ttgctcgccc aacagcatta tgagaatttc aaagaccgtt tcgccaatta
     6601 ccctgtgaat gtggaagtgc tgtctcgctt taaaaccgcc aaagagcaga aaacgatttt
     6661 gcaaaatgtg gcggagggca aagtggatat tttggtcggc acgcacaaac tgttgcaaga
     6721 cgatgtagcg ttccgtgatc ttgggctttt agtgattgat gaagaacacc gtttcggcgt
     6781 acgccaaaaa gagaaaatca aacaactgcg tgccaatgtg gatattctga ccctcaccgc
     6841 cacgccaatt ccaagaacgc tgaatatggc actcaacgga atgcgtgatc tctcgattat
     6901 cgccagcccg ccggctcgcc gacttttaat caaaaccttt gtgcgacaaa gtgatgaaat
     6961 ggtaatccgt gaggcaattt tgcgtgaaat tctgcgtggt gggcaggttt actacctcca
     7021 taacgatgtg gcaaccattg aaaattgtgc cgagaaattg gctgaactcg tgccagaggc
     7081 aagaattgtg atcggacacg ggcagatgcg tgagcgtgaa ttggagcggg tgatgagcga
     7141 tttctatcat caacgcttta acctgctggt ttgctccacc attattgaaa cggggattga
     7201 cgtgccgact gccaatacca ttattatcga acgggcggac aaattcgggc tggcacaact
     7261 gcaccaactg cgtggacggg tcggacgctc gcaccaccaa gcctacgcct atatgctcac
     7321 accgcacccg aaaacgctca ccaaagatgc cgagcaacgc ttggaggcga tgagtaccat
     7381 tgataatctc ggggccggtt ttgcgttggc gacccacgat ttagaaattc gtggtgcagg
     7441 cgaattgctc ggttcggagc agagcggaca gattgaaagc atcggttttt cgctctatat
     7501 ggacttgctc gaaaatgcgg tcaaagcttt gcaagaaggg cgagaaccga gcctcgatga
     7561 aatcacccaa caccaagcgg agattgaatt gcgtgtaccg gccttattgc cggacgatta
     7621 tctgcctgac gtgaatatgc gtctgtcgtt ctacaagcgg atcgcaagct cggaaaatct
     7681 gcaaagcctg aacgacttga aagtggagct gatcgatcgc ttcggcttgc tcccagaggc
     7741 aaccaaaaac ctgtttgcga taacccaact tcgcttgtcg gcacaaacgc tcgggattaa
     7801 gaaaatcgat gccggcattc acggcggtta tcttgaattt aaaccgacag cccagcccga
     7861 cccaatgaag tttatcgcct taatccaaaa acaaccagcg gtgtacagtt ttgaaggggc
     7921 ggtgaagttc aaattccgct tagcactcga agacaaccgc aagcggttag aatttgtgga
     7981 aaatttgcta agggaggttg tgggcggata aagcctccta aggttgtcaa tcttgaaaaa
     8041 cgatttttgc gatctgtatc gtaaaaatcg tttttgttta ttgcgaatcc agtaatttca
     8101 tctaggctgt ttataactta aaaaaataga tgtaaatata ttttatttac aatcaaaaag
     8161 gagtaaactg gcaatggaag aaattaatgg ttttgaatgg gatagtgaga aagctcttcg
     8221 taatcaacaa aaacacggag ttagttttat cgaggcggta agcgtatttt atgataataa
     8281 tgcgatgtta atcccggatc cagatcactc atctagggaa gagcgttttt tgctactcgg
     8341 gcgtagcaat aaagataatc tacttgtggt tgtgcattgc gagagaaata ccaacattcg
     8401 gattatttca gcacgtttgg caacaaaacg cgaaagtggg caatataaag gagtacatta
     8461 aatgagagat gaatacgatt tttctaatgc agtgaaaaac ccttacgcaa aagggctaaa
     8521 aagcaaaagt gtggttacta ttcggattga agacgatgtt atcagctatt ttaaacaact
     8581 tgccgaagag gtgaatttgc cttaccaaac gttgatcaat ctttatttga aagattgcgt
     8641 tgagcagaaa cgtaaaccga ctattagttg gtaatattat gatttgaaat cagtatgtta
     8701 ttgagtattt gagaatgggt tatcttgcca gttgtataaa ataggcatat actttggcat
     8761 atacactatc tatcggaggg taaagtaaga tggaaaaaat tgcgagatta ttccgtaacg
     8821 ggcggaacca agcagtgcgt ttaccggttg aatttgagtt tgatgcagag caagtctatg
     8881 tttctaaaaa agaaaatggc gatattttat tatcattgag atcgagaaat accgagcatt
     8941 ggcaacgatt gtttgagcta ttgcctgctg ttgtatgtga tgaaacattt ttagatacaa
     9001 aagagagaaa ccaatccttt gagcaacggg atccttttga ggtagcttaa atgtatatgt
     9061 tagatacgaa tactgtaagc tatttttttc gtggaaatgc aaatgtggtg gcgaaattga
     9121 agtccattaa tcctgagagg ctttgtattt catctgtaac tgctgctgaa ttggtttatg
     9181 gtgtggcgaa aagaaataat gggcagcttt ctcaattttt agattatttt ttatcgagtg
     9241 taaaggtttt agattggaat tatcgttgtg ctgaacttta tggaaagtta cgggcagaga
     9301 tggagaaaaa tggcaaagta atgggcgtgc aggatcaaat gattgctagt cacgctcttg
     9361 ctgaagagtg tattttagtt tctagtgatc aggcatttaa aatggtacct gatctgaaat
     9421 tggagaattg gttacactaa actatagtaa ggaaaaacaa tgaaacaatc ccttcttaac
     9481 cgtttactac gttacaccca aattcacaca acttcagatc cgaaaagcca aactgtgcca
     9541 agctcgcaac agcagtggga tttgcttcat ctgctgaaaa cggaagtaga ggcgatgggg
     9601 ctaagcgaga tttatcttga ccccaacggc tatctgttcg ccagcttgcc tgccaatatt
     9661 gacaaaaccc tgccggtgct tgggctgatt gcccacgttg atacttcgcc cgattttaat
     9721 ggtgaaaacg tgaaaccaca ggtgattgag gcttatcaag gcggcaatat tttgctaaac
     9781 ggcagtggcg attatctttc gccaacggat ttcccgaatt taaacaagct gattggcgac
     9841 accttagtca ccactgacgg cacctctttg cttggcgcag acgacaaatc cggcattgcg
     9901 attattctct cggctatcga gtatttacaa gccaatccgc atattccgca cggcaagatc
     9961 cgcatcggct ttaccaccga tgaagagatc ggtcgaggcg cagatcattt tgatgtaaac
    10021 gcattcggtg ccgatgtcgc ctttaccttt gatggcgggc ctctgggcga attgcaatat
    10081 gaaaatttca atgccgatgc agcgaccgtt acgttcttcg ggcgtagcgt tcaccccggc
    10141 acggcaaagg gcaagatgat caacgcctta gagcgagctt gcgagttcca acaccgtctg
    10201 cctccccatt taacccctgc caccagcgaa gggcgtgaag gctttattat gttgcacggc
    10261 attgagggtg gaattgataa agcggaaatt cactatattt tacgtagctt tgagcgtgat
    10321 gaattagctg cattcggcaa aatgctgaat gatacgctgg cagagatgca acagatttac
    10381 ggcgaacatt gcggaaaagt cgagattgtc gagcaatatc gcaatatgcg tgaagtgctg
    10441 gacgattacg gctacttggt tgaaattgcc gaaaacgcaa tgcgtgaaca aggcattgag
    10501 ccgaatttag aaccgatccg tggcggtact gatggctcac gcctttcatt tatgggcttg
    10561 ccttgcccga atttatttac cggcggcgaa aatttccacg gacgatttga gtttatttcg
    10621 gttgatacga tggaaaaagc cacaaaagtt gtaattgata tttgtcggaa atttgctgat
    10681 tatgatttca acaaataagg cggctgcaag cggtcaaatt tgatgaaaaa tttgcaaaaa
    10741 gcgttatttt tcaccaatta aacgattttt tacagatttc ctacaaattt atttgacatt
    10801 aaatctgaaa tccatagaat atgcacctgt tgagacctct acgaggttcg tagcgttttt
    10861 aa
//
LOCUS       DANLZC010000088        10663 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.088, whole genome shotgun sequence.
ACCESSION   DANLZC010000088 DANLZC010000000
VERSION     DANLZC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.088"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            149..2716
                     /gene="clpB"
                     /locus_tag="PXE44_001717"
     CDS             149..2716
                     /gene="clpB"
                     /locus_tag="PXE44_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL5589213.1"
                     /translation="MNFEKMTTKLQEALAESQSLAVGKDNPYIEPAHLLYALLKQEGG
                     SIASLFTTLNVDVPTLIRELQQILDRLPKVQGGNTQVSQQLVRLLNQSDKLAQQFGDS
                     FISSELFVLAALDDNGDLGKLFKQFGLNKEKLTQAISQIRGGDTVNNQNAEDTRQALK
                     KYTIDLTERAKAGKLDPVIGRDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGEVPEGLKNKRVLSLDMGALIAGAKYRGEFEERLKAVLNDLAKEEGQVILFID
                     EIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYRQYIEKDAALERRFQKV
                     LVDEPTVEDTIAILRGLKERYEIHHHVQITDPAIVAAATLSHRYVSDRQLPDKAIDLI
                     DEAASSLRMEIDSKPEPLDKLERRIIQLKLERQALQKEEDEASRQRLAKLDEEMAAKE
                     REYSELEEIWKAEKSALLGTQHIKSELENARIAMDQARRENDFEKMSELQYGVIPNLE
                     KQLQAVENGEKNADAQLLRTKVTEEEIAEVLSKATGIPVAKMMEGEKEKLLRMEEVLH
                     ARVIGQNEAVDAVANAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELSKTLANFLFDD
                     PDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRHPYSVVLLDEVEKAH
                     PDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSHLIRENAYKGYDVVKEMVM
                     EVVGQHFRPEFINRIDETVMFHSLGQEHIRSIAKIQLQRLIQRLAERGYEVTVTDAAL
                     DHIGKAGFDPLFGARPLKRAIQQELENPLAQQILSGKLLPNKAVTVDYQNGQIVATQ"
     gene            2930..4072
                     /locus_tag="PXE44_001718"
     CDS             2930..4072
                     /locus_tag="PXE44_001718"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL5589214.1"
                     /translation="MSQLFPEATIRTQAPYRNDIVGSFLRTDALKKAREDFNQQKITR
                     DELREVTRQEVKKLVELQKQHGVKAVSDGEFSRTWWHLDFLAELDGMDWQETEVFNVN
                     FTGHKPKGQTVKIVGDIDFSESHPFVDAYRLLKEAAGDYPTKFTIPAPTMLHIICCVR
                     AEDYQPLPQYQDEQKLYDDIANAYIKAMHKFYDMGLRNLQLDDTSWGQFCAEDKRKEF
                     AARGFNLTDLAEKYVALLNRIVDAKPADMSITMHICRGNFRSSWFSEGGYGPVAEVLF
                     GKCRVDGFFLEYDTERAGTFEPLRHIKDQQVVLGLITSKFGDLEDKNVIIERIKEAAQ
                     IVPINQLCLSPQCGFASTEEGNILTEAAQWAKLNLIKEIAEEVWGS"
     gene            complement(4129..5520)
                     /locus_tag="PXE44_001719"
     CDS             complement(4129..5520)
                     /locus_tag="PXE44_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDL5589215.1"
                     /translation="MENTPLIEPSKNTRNSLIFVADAILFIILLNTLPFTPEANKGLA
                     LLIFIAVLWLTEALHVTVTALLIPILAVALGLVKSKEALVAFADPTIFLFFGGFALAT
                     ALHIQQLDRMIANKIMALAKGKLSIAVIYLFAVTAFLSMWMSNTATAAMMLPLAMGIL
                     SKLDQKQNHNTYLFVLLGIAYSASIGGMGTLVGSPPNAIVASQLNLTFADWLKYGLPI
                     MLILFPLMIATLYIVFKPNFSVSFDRSFEKIELNRSRIITLAIFVFIALGWIFGDKIN
                     PIISAFLGINGKIASFDTILALIAAALICITRVANWQQIQENTEWGVLFLFGGGLTLS
                     AVLGQTGASKIMADGVVSLIEGGHFYLIGLIVAAFIIFLTEVTSNTASAALLVPIFIS
                     IAQALNMPPVGLALIIGLGASCAFMLPVATPPNAIVFGTGQIKQSEMIKVGFWLNIIS
                     IFVIATVGYLFWL"
     gene            complement(5680..6579)
                     /gene="cdd"
                     /locus_tag="PXE44_001720"
     CDS             complement(5680..6579)
                     /gene="cdd"
                     /locus_tag="PXE44_001720"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL5589216.1"
                     /translation="MFRTDITERFEQLKAAKNCEVTQAIIEQLIKQDFHGQLSYEVVD
                     ELCEKFKRSRVELALYCIAIAACYAVTPVSDFNVGAVAIGKNGDFYFGANQEFSGECM
                     QQSVHAEQSAISHAFLAGETLITDVVVNYTPCGHCRQFMNELNSAASLKVHLPHSQNN
                     LLHSYLPDSFGPKDLGIEKVLFDEQPQQFEVKGDHLQQAAIFAANNAYAPYSNAFSGV
                     ALQVGERIVCGKYTENAAFNPTFLPLQSALNYRLFLGLADIPVTRVVLAEAKGSLSSY
                     AMTQALAKSLFGLEIEYLSLQAV"
     gene            complement(6593..7495)
                     /locus_tag="PXE44_001721"
     CDS             complement(6593..7495)
                     /locus_tag="PXE44_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5589217.1"
                     /translation="MISSSLKLGYFSLIVATLFWGGNFVLGKVLSGAVQPITLTYLRW
                     LPAFLILVLLFFRPTWRVMHLLKPRLGVMWVLGLLGVILFPATLYEGLKTTTALNASL
                     YLAISPVLVLFLNRLVFKEKISPIILSGAIISLLGVLWLLMQGKLTRLFSLAINQGDL
                     WAIASTISWAVYCCVIRLRPQGISNTVFLTVLVGLAVLTLTPFFIYEYVAESAKNLAN
                     LTALQWSGIGYLVIGPSILSYAFWNFGIAIVGSAKGAVMTNFTPLFAAMFSIILLNES
                     VHTYHIISAILITAGILICSYKKH"
     gene            complement(7492..7848)
                     /gene="folB"
                     /locus_tag="PXE44_001722"
     CDS             complement(7492..7848)
                     /gene="folB"
                     /locus_tag="PXE44_001722"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDL5589218.1"
                     /translation="MSDKVFIRELTAFASIGAYNWEHTIKQRLVFNIDMAWDFSKAAE
                     TDDVQYCLNYAEVSQKVLDFVESQPFKLVETVAYQLAELLQKEYQLSWIRIELHKPKA
                     VAQASSVGVIVERGHQ"
     gene            7918..8523
                     /gene="plsY"
                     /locus_tag="PXE44_001723"
     CDS             7918..8523
                     /gene="plsY"
                     /locus_tag="PXE44_001723"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL5589219.1"
                     /translation="MNLIPYLLILTAYLLGSISSAVIFCKLAGLPDPREVGSHNAGAT
                     NVLRIGGKSAALGVLLFDILKGTLPVAMGIYFELSPSQLGFIALAVCLGHIFPIFFQF
                     RGGKGVATAFGAISALSFIVAGGAIGTWLLVFLISGYSSLSAVFTALVVPFYIWWFEP
                     QFTFPVALVCCLLVYRHHDNIQRLWRGQEDKLWDKFKKNRA"
     gene            complement(8597..10504)
                     /gene="parE"
                     /locus_tag="PXE44_001724"
     CDS             complement(8597..10504)
                     /gene="parE"
                     /locus_tag="PXE44_001724"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL5589220.1"
                     /translation="MSEKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNS
                     VDEALSGFADKIDVILHADNSLEVVDNGRGMPVDIHSTEKISGVELILTKLHAGGKFS
                     NKNYTFSGGLHGVGISVVNALSERVEIQIKRGGEVYSIAFANGVKVEDLTVIGTCPKK
                     QTGTTVRFYPNPKYFDSPRFSVSRLRHLLRAKAVLCPKLTINFIDKINNTEESWYYED
                     GLSDYLSEALNGYETVPNPPFIGDVTAETEAVSWALTWLPEGGELVAESYVNLIPTAQ
                     GGTHVNGLRNGLLKAMVEFCEIHNLLPKGVKLTADDVWNRCAYVLSLKIQEPQFAGQT
                     KERLSSRQASSYVDSTLKDAFSLWLNQNVQTGKLIAEMAISSAQSRMRAAKKVVRKKL
                     VSGPALPGKLADCTAQDLSRTELFLVEGDSAGGSAKQARDKEYQAILPLRGKILNTWE
                     VSSDQVLASQEVHDIAVALGIDPDSDNLEELRYGKVCILADADSDGLHIATLLCALFL
                     RHFPKLVQNGHVYVAMPPLYRIDIGKDEVHYALDEAEKEAILARLAKKKGKPNVQRFK
                     GLGEMNPSQLRETTMEPSTRRLVQLTFDDTVEEGELNTFEIMDMLLGKKRAEDRKQWL
                     QNRGDEADLAV"
BASE COUNT         3313 a         2335 c         2124 g         2891 t
ORIGIN      
        1 aaagataccc aacaagagtt attttattta tttttgtgat ctttttcaca tttttacttg
       61 aaaattgttt accaagctcc atttagtgtt tcaacaagag atgtaaataa aaatttacac
      121 tttctaaact cattgtaagg agaaagaaat gaattttgaa aaaatgacca caaaactgca
      181 agaagccttg gctgaatcac agtcgcttgc ggtaggaaaa gataatccgt acatcgaacc
      241 ggcacatttg ctctatgcgt tgttaaaaca agaaggtggc tcgattgctt ctttgtttac
      301 cacattaaat gttgatgtac cgactttaat tcgtgaatta caacaaattt tagaccgctt
      361 gccgaaagtt caaggcggta acacccaagt ttcacaacag ttggtcagac tgttaaatca
      421 atcagacaaa ctggcacaac aattcggcga cagctttatt tcatcagaac tatttgttct
      481 agctgcactt gatgacaatg gcgatttggg gaaactattt aaacaatttg gcttaaacaa
      541 ggaaaaacta acacaagcca tcagccaaat tcgaggagga gacacagtga ataatcaaaa
      601 tgcggaagat acccgccaag cactcaaaaa atatacgatt gatttaaccg aaagagccaa
      661 agccggtaaa ttagacccgg ttatcgggcg tgatgaagaa attcgccgtg cggtgcaagt
      721 cttacaacgc cgtaccaaaa acaaccctgt gttaattggt gaaccgggtg tggggaaaac
      781 ggcgattgta gaaggcttgg cacagcgtat tgtaaacggc gaagtgccgg aaggcttgaa
      841 aaataagcgt gtactttcgc tggatatggg agcattaatt gccggtgcga aatatcgtgg
      901 tgaatttgaa gagcgtttaa aagcggtttt aaacgacctg gcgaaagaag aagggcaagt
      961 tatcctcttt attgatgaaa ttcacacgat ggttggagca ggcaaaacag acggagcaat
     1021 ggacgccggc aacttattaa aaccaagcct tgcccgtggt gaattgcact gtgtgggggc
     1081 tacaaccctt gatgaatatc gccaatacat tgaaaaagac gcagcccttg aacgccgttt
     1141 ccaaaaagta ttggtggacg aaccaacggt tgaagatacg attgcgattt tacgtggttt
     1201 gaaagagcgt tatgaaatcc atcatcacgt tcaaattaca gaccctgcca tcgtggcggc
     1261 ggcaacgctt tcgcaccgtt atgtgtctga ccgtcaattg ccggataaag cgattgactt
     1321 gattgatgaa gccgcttcaa gcctgcgtat ggagattgat tctaaacctg agccgttgga
     1381 taaattagaa cgccgcatta tccaactcaa attggaacgc caagcgttac aaaaagagga
     1441 agatgaagcg agccgtcagc gtttagccaa attagacgaa gaaatggcag cgaaagagcg
     1501 tgaatattcc gagctggaag aaatctggaa agcggaaaaa tccgccttac ttggtacaca
     1561 gcatattaaa tcggagttgg aaaatgcccg aattgcgatg gatcaggctc gccgtgaaaa
     1621 cgatttcgag aagatgtcgg aattgcaata tggcgtgatt ccaaacttgg aaaaacagtt
     1681 acaagcggtc gaaaatggcg aaaaaaatgc agatgctcag ctcctccgta ctaaagtcac
     1741 cgaagaggaa attgcggaag tgctttcaaa agcaacgggc attcctgttg caaaaatgat
     1801 ggaaggcgag aaggaaaaac tccttcgtat ggaagaagta ttacacgctc gggttatcgg
     1861 gcaaaatgaa gccgttgatg cggtggcaaa tgcgattcgc cgtagccgtg ccggactttc
     1921 cgatccgaat aaaccgattg gttcattcct attcttagga ccgaccggcg tgggtaaaac
     1981 cgagttaagc aaaaccttag ctaatttctt gtttgacgat cctgatgcta tggtgcgaat
     2041 tgatatgtct gaatttatgg aaaaacacag cgtatcccgc ttagtgggcg cacctccggg
     2101 ctatgtcggc tacgaagaag gcggctattt gaccgaagcg gttcgccgcc atccatattc
     2161 ggtagtgtta cttgatgaag tggaaaaagc tcaccctgat gtgtttaaca ttttattgca
     2221 agtgttagat gacgggcgtt taaccgacgg gcaaggcaga acagtcgatt tccgcaatac
     2281 ggtagtgatt atgacctcaa acttaggctc gcatttaatt cgagaaaatg cgtataaagg
     2341 ctatgatgtg gttaaagaaa tggtgatgga agtcgtcgga cagcacttcc gccctgaatt
     2401 tatcaaccgt attgatgaaa cggtgatgtt ccactcactt ggtcaagagc atatccgttc
     2461 gattgcgaag attcagctac aacgcttaat ccaacgcttg gcagaacgag gctatgaggt
     2521 gacagtaacc gatgcagcat tagatcatat cggcaaggct ggtttcgatc cgctgttcgg
     2581 agcaagacca ctgaaacgtg cgattcaaca agagcttgaa aatccgcttg ctcaacagat
     2641 cttaagcggt aaattattac cgaataaagc cgttacggtg gattaccaaa acgggcagat
     2701 tgttgcaaca caataatgtc tccgatggtg cgagcgtcct cgctcgtacc ttacaagcgg
     2761 tggatttatc gtaaaaattt gcaaaaaatt gctcgaaaat aaccgcttgc acagtaaaaa
     2821 ttttcatatt gcccttcatt aattttcata agctaatgaa gggcttttta ttactctttt
     2881 tgctatcatt aacggcgttt ataaccaata caattacaag gagtaattta tgagccaatt
     2941 atttccggaa gcaacaatcc gcactcaagc cccgtatcgt aatgatattg taggcagctt
     3001 tctacgtacc gatgcgttaa aaaaagccag agaagatttc aatcagcaaa aaatcacccg
     3061 tgacgaattg cgtgaagtca cacgccaaga ggtcaaaaaa ttagttgagt tgcaaaagca
     3121 acacggtgta aaagcagtct ctgacggtga atttagccgg acttggtggc atttagattt
     3181 cttagctgaa cttgatggaa tggattggca agaaaccgaa gtctttaacg tgaactttac
     3241 cggtcataaa cccaaagggc aaacggtaaa aatcgtgggc gatattgatt tctccgaaag
     3301 ccaccctttt gtggacgctt atcgtctttt aaaagaagct gccggcgatt acccaaccaa
     3361 attcacgatt ccggcaccga caatgttaca tattatctgc tgtgtgcgtg cggaagatta
     3421 ccaaccgtta ccacaatatc aagacgaaca aaaactgtac gatgatattg ctaatgctta
     3481 tatcaaagca atgcacaaat tctacgatat ggggctacgt aacttacaat tagacgacac
     3541 cagttgggga caattctgtg ccgaagataa acggaaagag tttgcagcca gaggatttaa
     3601 tttaactgat ttagcggaaa aatatgtcgc attattaaac cgtattgttg atgcaaaacc
     3661 ggcggatatg tcaattacga tgcacatctg ccgtggtaac ttccgttctt cttggttctc
     3721 ggaaggcggt tatggaccgg ttgccgaagt attattcggt aaatgccgtg ttgacggctt
     3781 cttcttagaa tacgataccg aacgtgcagg cactttcgag ccactacgcc acattaaaga
     3841 tcaacaagtg gtactaggct taattacctc taaatttggc gatttagaag ataaaaatgt
     3901 gatcattgag cgtattaaag aagcggctca aattgtgcca atcaatcagc tatgcttatc
     3961 accacaatgc ggctttgcct ccaccgaaga aggcaatatt ttaaccgaag ccgcacaatg
     4021 ggcgaaacta aacttaatta aagaaattgc ggaagaagtt tggggtagct aatcctctat
     4081 aatacaaata gccgagtaaa tactcggcta ttttagtttg ttttaacatt acaaccagaa
     4141 gagataccca acagtcgcaa tcacaaagat agagataata tttagccaga aaccaacttt
     4201 aatcatctca ctttgtttaa tttgccctgt accaaataca atcgcattcg gtggggtggc
     4261 aaccggcaac ataaaggcgc aagatgctcc tagaccaata attaaggcta atccgaccgg
     4321 tggcatattt aatgcttgtg ctatagagat gaaaatcggc actaacaaag ccgcactggc
     4381 tgtgttagag gtaacctcag ttaggaaaat aataaatgct gccacaatta aaccaattag
     4441 gtaaaaatgc cccccttcaa ttagtgagac gaccccatca gccataattt tgctcgcacc
     4501 agtttgtcct aatacagcac ttaacgtcaa accaccacca aataggaata atacccccca
     4561 ctcggtgttt tcttgaatct gttgccaatt tgcgacacga gtaatgcaaa taagtgctgc
     4621 ggcaatcaat gcaagaatgg tgtcaaagct cgcaattttg ccattaattc ccagaaatgc
     4681 agaaataata gggttaattt tatcaccaaa aatccaacca agcgcaataa agacgaaaat
     4741 agccaaggtg ataatacgag aacgatttaa ttcaattttc tcaaatgatc gatcaaaact
     4801 aactgaaaaa tttggtttaa atacgatata tagtgttgca atcattaatg ggaataaaat
     4861 tagcataatt ggcaaaccat attttaacca atcagcaaaa gtcagattca attgagaagc
     4921 aacaatcgca ttcggtgggc ttcccactaa agtacccatt ccaccaatac tggcactata
     4981 agcaattcct aataacacga aaagataagt attatggttt tgtttctgat caagtttact
     5041 taaaatcccc attgccaaag gtaacatcat tgcagctgta gctgtattac tcatccacat
     5101 tgacaaaaac gctgttaccg caaataaata aataactgca atcgataatt tacctttagc
     5161 taatgccata attttattgg caatcatacg atctagctgc tgaatgtgta atgctgtcgc
     5221 taaggcaaag cctccaaaaa ataagaaaat cgttggatca gcaaaagcaa caagagcttc
     5281 ttttgattta accagcccca gtgctacagc caaaatcgga attaataatg ccgtaaccgt
     5341 aacgtgtaat gcttctgtta gccataagac ggcaatgaaa attaacaatg ccaacccctt
     5401 attggcttca ggggtaaaag gcaaggtatt taacaaaata ataaataaaa tagcatctgc
     5461 aacaaaaatc aagctattgc gtgtattttt actcggttca ataagaggag tattttccat
     5521 aaaaacctca caaaatatag ataatcagtg gtatcactat atataaaaaa acgatatagg
     5581 caaatataat tgttgcaaaa gtgtgaagct catcaaaaaa tttaacacta aaaaataaat
     5641 tgcttaaaga tacagcacct ttgcaaaaat aaatcaaaat tacaccgctt gtagcgaaag
     5701 gtattcaatc tctaacccaa atagtgattt tgccaatgct tgagtcattg cataactact
     5761 gagagagcct ttcgcctcag ccagcacaac tcgagtaacg ggaatatcgg caagccctaa
     5821 aaataatcga tagttcaacg cgctttgcaa cggtaaaaaa gtaggattaa aagctgcatt
     5881 ctcagtatat ttaccacaaa caatacgctc acctacttgc agtgcaaccc cactaaatgc
     5941 gttgctgtaa ggggcataag cattattggc agcaaaaatt gccgcttgtt gtaaatgatc
     6001 gcccttaacc tcaaattgtt gcggttgttc atcaaataag actttctcaa tccctaaatc
     6061 tttcggtcca aaactatccg gcaaatagct atggagcaaa ttattttggc tatgcggtaa
     6121 atgtactttt aagcttgccg cactgtttaa ttcattcata aattggcggc aatgcccaca
     6181 aggggtatag ttcacaacca catctgttat caaagtttcc cctgctaaaa atgcgtgcga
     6241 gattgcactt tgctctgcgt gaacggattg ttgcatacat tccccggaaa actcttggtt
     6301 tgccccaaaa tagaaatctc cgtttttgcc aatcgcaacc gcccctacat taaaatcgga
     6361 aactggtgta accgcataac aggcagcaat ggcaatgcaa tataaagcca actcaacacg
     6421 agagcgttta aatttttcac agagttcatc aaccacttcg taagagagct gcccgtgaaa
     6481 atcttgcttg ataagttgct caataatagc ttgtgtaact tcacagtttt tcgctgcttt
     6541 taattgttca aaacgctctg taatgtctgt tcgaaacata ctgtacctcc gtttaatgtt
     6601 ttttatagct gcaaatcagt atccctgccg taatcagtat ggcactaata atatggtaag
     6661 tatgaactga ttcatttaat aaaataatac tgaacattgc tgcaaagagc ggcgtaaaat
     6721 tagtcattac cgctccttta gcagaaccga ctattgcaat gccgaaattc caaaacgcat
     6781 aagagagaat agaagggccg ataaccaaat agcctatccc actccattgt aaagcggtca
     6841 aatttgctaa attttttgca gattctgcta catattcata gataaaaaat ggcgttaacg
     6901 ttagaactgc cagccccact aatacggtca aaaacaccgt attgcttata ccttgtggac
     6961 gtagccgaat cacacaacaa taaaccgccc agcttatagt agaagcaatc gcccataaat
     7021 caccttgatt aattgccaag ctgaataagc gagttagctt cccttgcatc aatagccaca
     7081 gcactcccag caaactgatt attgccccac ttagaataat cgggctgatt ttctccttaa
     7141 aaacgagccg atttaaaaat agcaccaata caggcgaaat agctaaatag agactcgcat
     7201 tcaatgctgt tgtcgttttt aaaccttcat ataaggtagc cgggaataaa attaccccaa
     7261 gcagacctaa tacccacatc acacctaaac gaggttttaa taagtgcatt acccgccaag
     7321 ttgggcgaaa aaagagcaaa actaaaatta aaaaagccgg caaccaacgc aaataggtta
     7381 aggtaatagg ctgaactgcc ccacttaaca ctttgcctaa tacaaaattc ccaccccaaa
     7441 aaagagtagc aacgattaaa ctgaaatacc ccaatttcag tgatgatgag atcattgatg
     7501 acctcgttct acaatcacac ctacactgct tgcttgggca accgctttcg gcttatgcag
     7561 ttcaatccga atccaagaaa gttgatactc tttttgtaat agttccgcca gttgataggc
     7621 aacagtttca actaatttga aaggttgaga ttcaacaaaa tccagcactt tttgcgatac
     7681 ttccgcataa ttcaagcaat attgtacatc atcggtttct gccgctttgg aaaaatccca
     7741 cgccatatca atgttaaaaa ccaagcgttg cttaatcgta tgctcccaat tgtaagcccc
     7801 aatggaagca aaagcggtta gttctcgaat aaaaacctta tcactcattt ttcgtcctgt
     7861 tttattttct gaatttctgt aaaatatagc acaagataaa tttaatcagg aaaatttatg
     7921 aatttaattc cctatttatt gatcttaacc gcctatttgc tcggctcgat ttccagtgcg
     7981 gtcatttttt gtaagctggc aggcttgcct gacccaagag aagtcggctc acacaatgcc
     8041 ggtgcaacga acgtactacg aattggcggt aaaagtgccg cattaggtgt gcttttattc
     8101 gatattttaa aaggcacatt accggtggcg atggggattt attttgaact ctctccctca
     8161 caactcggtt tcatcgcctt agcggtttgt cttgggcata ttttcccgat tttcttccaa
     8221 ttccgtggcg gaaaaggcgt tgccaccgca tttggtgcaa tctccgcatt aagctttatt
     8281 gttgcaggcg gtgctatcgg cacttggcta ctggtgtttt taatctccgg ctattcttct
     8341 ttaagtgccg tcttcactgc cttggtcgta ccgttttaca tttggtggtt cgagccacaa
     8401 tttactttcc ccgtcgcctt agtttgttgc ctattggttt accgccatca cgataatatt
     8461 caacgcttat ggcgtgggca ggaagacaaa ctgtgggata aattcaagaa aaatagagca
     8521 taaaaatagg catagtgaac cgttcgctat gcctatttgc aaattattgc ggaaaaatca
     8581 ccgcttgtct tctcgactaa accgctaaat ctgcttcatc tcctcgattt tgtagccatt
     8641 gtttacgatc ttccgctcgt tttttaccca atagcatatc cataatttca aaggtattca
     8701 attcgccctc ttctaccgtg tcatcaaagg ttaattgtac taagcggcga gtgcttggct
     8761 ccatcgtagt ttcacgcaac tggctcgggt tcatttcgcc taagccttta aagcgttgca
     8821 catttggctt tccttttttc ttggcaaggc gagctaaaat cgcctctttt tccgcctcat
     8881 ctaaggcata atgcacctca tctttgccga tatcaatgcg ataaagtggt ggcattgcca
     8941 cataaacgtg tccgttttgc accaatttcg ggaaatgacg aaggaataac gcacacagca
     9001 aggtggcaat atgcaaaccg tctgaatccg catcggcaag aatacaaact ttgccataac
     9061 gcaactcttc taaattatcg ctatcagggt caattcccaa ggctaccgca atatcgtgta
     9121 cttcttgtga agccaatact tggtctgatg aaacctccca agtgtttaaa atcttaccac
     9181 gcagtggcaa aatggcttga tactctttat ctcgagcttg ttttgccgaa ccgccggcag
     9241 aatcaccttc taccaagaaa agttcggtac ggctgagatc ttgagctgta caatccgcca
     9301 gtttgcccgg taaagccgga ccactcacca atttcttacg caccactttt ttcgccgcac
     9361 gcatacggct ctgggcggaa ctaattgcca tttcagcaat taatttacct gtctgcacat
     9421 tttggttaag ccataaacta aaggcatctt tcaaggtgct atccacatag cttgaggctt
     9481 gacgggaaga caaacgctct ttggtctgcc ctgcaaattg tggctcttga attttcagag
     9541 aaagcacata agcacaacgg ttccacacat catcagcggt taattttacc cctttcggca
     9601 ataaattatg gatttcgcaa aactccacca tcgcctttaa taaaccgtta cgcaagccat
     9661 taacgtgcgt gccgccttgt gcggtcggaa tcaggttcac atagctttcc gctactaact
     9721 cgcccccttc cggcaaccaa gttaatgccc agcttaccgc ctctgtttca gcagtaacat
     9781 caccaataaa cggtggattc ggcacggttt cataaccgtt taatgcctcg ctcaaataat
     9841 cagataagcc gtcttcgtaa taccaacttt cttcggtatt gttgatttta tcaataaaat
     9901 tgatggtgag tttcgggcaa agcaccgctt tggctcgtaa caaatggcgt aaacgactca
     9961 ccgaaaaacg agggctatca aaatattttg ggttcgggta aaaacgcact gtcgtgccgg
    10021 tctgtttttt cgggcaagta ccaattaccg tcagatcttc cacttttacc ccgttggcaa
    10081 aggcaatcga atagacttct ccgccacgtt taatctgaat ttcaacccgt tcggacaggg
    10141 catttaccac cgaaatcccc accccgtgca aaccaccgga aaacgtatag tttttattgg
    10201 aaaatttacc gccggcgtgc aatttcgtta agataagctc cacccctgaa attttctcgg
    10261 tggaatgaat atcgaccggc attccccgcc cgttatccac cacttccaac gaattatcag
    10321 catgaagaat cacatcaatt ttatctgcaa agccggacaa cgcctcatcg acactattat
    10381 caatcacctc ctgccccaaa tggtttggac gtgtggtatc cgtgtacatt cccgggcgaa
    10441 gctgcaccgg ctcgaggtct ttcagaacgg taatttcatc cgccccatag cgtttttcag
    10501 acattatgct ttctcttagg cttaaaataa aataaggctg aattttaagg aatattcagc
    10561 tattttgcaa aattttggtg aaaaatgacc tgttgttatg caataaaaac tatttttgcg
    10621 atctacatcg aaaaaatgag ttttaatttc cctatttgct tat
//
LOCUS       DANLZC010000089        10450 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.089, whole genome shotgun sequence.
ACCESSION   DANLZC010000089 DANLZC010000000
VERSION     DANLZC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10450
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.089"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            147..223
                     /locus_tag="PXE44_001725"
     tRNA            147..223
                     /locus_tag="PXE44_001725"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:181..183,aa:Met,seq:cat)
     gene            374..1834
                     /gene="xseA"
                     /locus_tag="PXE44_001726"
     CDS             374..1834
                     /gene="xseA"
                     /locus_tag="PXE44_001726"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL5589221.1"
                     /translation="MSNILTVTQLNYSVRHLLEMEMSHIWLTGEISNFSQPVSGHWYL
                     TLKDDKAQVRCAMFKMKNVRVNFRPQNGMQVLVRASVTLYEPRGDYQLIIESMQPAGE
                     GLLQQQFEQLKAKLSAQGLFAQEHKKPIPTFVKTVGIITSSSGAALQDILHILQRRDP
                     SLSVVIYPALVQGKEATQDIVNMIELANRRNECDVLIVGRGGGSLEDLWCFNEESVAY
                     AIYHSQIPIISAVGHETDVTIADFVADLRAPTPSAAAELVSRDQNELKRQLQHQFDKV
                     NLAFDRVWNHKFNRFQQLPLRLNAQHPSKQIQSQQQRFDYMEYRLEQAIYALVAAKKQ
                     VGKELNVRLIHQHPQKQIERQSQMLKQLELKLHWQMEQQLSKKQQKWKNITQRFERNP
                     LPYQLYQQQTMVAKMSQRLAYSIEKIMLKEQRHFQELCTKLDGLSPLKILTRGYSITQ
                     TKDGKTLTSTENIERGEAIITQLKTGKIISQVVDFL"
     gene            2146..2231
                     /locus_tag="PXE44_001727"
     tRNA            2146..2231
                     /locus_tag="PXE44_001727"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2180..2182,aa:Leu,seq:caa)
     gene            2329..3153
                     /locus_tag="PXE44_001728"
     CDS             2329..3153
                     /locus_tag="PXE44_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL5589222.1"
                     /translation="MKPLQNGTNKPFTITIASTKGGSAKSTNAANIGAFCAEHGLRTL
                     LIDTDTQPTLSSYYELEYQAPGGTYEFLHFKDVDPTHIISRTTIPNLDLIQSNDPSNK
                     ISPMLRDSPDGALRFSLLLQKIEGYDVIIIDTRGTRDITVDMSVLAADVLFCPILPHI
                     LSAKEFIRGTIGMYQDLETFTAFGFTLPPLKAMINCVDHTKDVKLVSDYLHTLFQNEF
                     DENKTLLEFSVPDRVAYREAATFSKPVYRQSQAEYETISQLCALLMPQFANSHFSA"
     gene            3165..4526
                     /gene="dnaB"
                     /locus_tag="PXE44_001729"
     CDS             3165..4526
                     /gene="dnaB"
                     /locus_tag="PXE44_001729"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL5589223.1"
                     /translation="MSSLNKSLVQPHSIEAEFSVIGGLLLDNELFDEVSSKINEGDFY
                     NFTHRLLFKHISELIEAGKPADILTLDQYLTQKKVIEDLGGFAYLAEIVKNTPSTANI
                     HAYTDIVLLYSKQRQLLKLGQFIVDQTQSAKTPDKFEAVVDDIEKKITEFSLSDNTKS
                     AADLSDIFANMLSRMELSAKNGDPVTGTPTGIQGIDEATTGGQPGDLIVIGARPSMGK
                     TAFSQTIAYHTLKKFESAPVFYHSMEMPADQILQRFLAMRARVGLQNIRQADSLDDEE
                     WSKLTSAMGYILEHWKNRLVIDDEGSLTPQKLRTKVRQNARKYGKPAAIFIDYIQLMQ
                     TSQRYENRNLEIANISRSLKLLAKEMDCPVYALSQLNRSVEQRANKRPINSDLRESGS
                     LEQDADVILFIYRDEVYDADSPFKGTAEIIIGKQRNGPLTSVMTNFRSEFALFENRTN
                     TTH"
     gene            4535..6181
                     /locus_tag="PXE44_001730"
     CDS             4535..6181
                     /locus_tag="PXE44_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5589224.1"
                     /translation="MTKDEKRLAAIAKGLNVSPISNTAPSYQTATPTHYSSDVEYITV
                     TLDKLRPYEHNPRKTRNPNFEMIKESIRRRGLDHKPNITRRPGEDFYIIADGGNTRIQ
                     ALKELFTETKDPKFWSISCQYKPWQGDTADSVEAELNLLIGHLIENDTRADLSFIEKA
                     LGILQAKEYYEKKLGKSLSSRELSSELENDGYIISHALILKMERCVNYLYPHIPEVLF
                     SGLGHTQIDKLLAIRNNAEEVWAAHQFDTDVTFESLWSENLSKFNEATPFQAKEFQSE
                     LITAMVEALEGKVSFETLYLDIDLDEQKFKKIAAKQQEIDNLVEKTVDQINTRQEQQT
                     AKSEPLIKNENKAVTPQKAQHIIDEVIDDNESDDEQNNAITDLSQLGSSLLDNQDNQE
                     DYLSQIAHNFGLTPGMSIQEQREQRAEANGLTFACEGRQPVEDIWLIHPARQYRSEAY
                     SLALDIAESMGIDHLVEHIVKQPVDYTYAMKPADTELSPSALTLYSFLSMLQTAEHKD
                     ENSCVLDSKFLFELDDLTLVKIFRLIRVSRHIRQQGGEND"
     gene            6174..6731
                     /locus_tag="PXE44_001731"
     CDS             6174..6731
                     /locus_tag="PXE44_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HDL5589225.1"
                     /translation="MIKPITVNEALIGNALLNIKEGNIHPCLKMGFSEEQLKAINNLS
                     IDEIMDISNSVVSFAKVEINHETFWKLLAIAQANTQQRQIIDRALLLGASIEMLHQYF
                     GLSTSEVSARRQLLGIEEKMGRKAAASDEESTHIWEIWQRYKQRMEILDSQEGLELLC
                     LIAEEGNMNLTVVWKLVSEWYSKHK"
     gene            6890..8080
                     /locus_tag="PXE44_001732"
     CDS             6890..8080
                     /locus_tag="PXE44_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589226.1"
                     /translation="MFSSTNKPTEHGLLFFGNQHETVPTRLLQDPCLTPRAKFAWQLI
                     KCNVQAFQGGLFPSYETLSKMLSDRPYAEAKLSEKVVTQTLYLLRLTRWLTLCETVRD
                     ERGYVQGNVYILHDEPVPILDAIQLNSDYLSFLDKCTKHKDSLINGVANHIVESLITD
                     KTKWHYVSHLDCLQSRYQIYQQQQLVKDKQPSQTEQELSKIQQNILTSTMEVRDFDEE
                     VRKKSSKNQTSNKGVRAKNEDKSLILDLLPLGKSDVQYRTLYINKYCTGEPSEITWPQ
                     EIPLTSLEQKLTAQAMKGLDLGLCQAILLEVQQRIIQKQDVKKPKGYLYTLIQRAKQG
                     EFKPYYFELGKNLPSQNQANNNSAHSIQKLTEQPTEKPVLTSADREQMASRMQKFRAE
                     LLLQ"
     gene            8259..9017
                     /locus_tag="PXE44_001733"
     CDS             8259..9017
                     /locus_tag="PXE44_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03761 family integrating conjugative element
                     protein"
                     /protein_id="HDL5589227.1"
                     /translation="MTTQLDPQLGPLRSEITFTLHTQYAHKLWVGRPMIRNEEGKVTQ
                     SSIISVPNCFAMLTQIQRAASEDDPYADDYLIQFEESVINYRKEIQKLTNDIVTLYAK
                     MLPEGISIERCANISPISYPIYVNSQLGYQLLYLLGDFDSLCRSVMTAAHIAIINRAE
                     AQDWIEAGARLIRKCFGIVERYKSSGITRRDYQEKNARYQAAIKRMGYTLSDAVLTGE
                     HRAEFAPFIKQNTTVEEEQSVETHITAQTNESQK"
     gene            9026..9511
                     /locus_tag="PXE44_001734"
     CDS             9026..9511
                     /locus_tag="PXE44_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HDL5589228.1"
                     /translation="MNLISDEIYRQLVKDSGLNTSLKQLFSHFDTGSDYELLQEQFTQ
                     ARAYFMAAQDSMVQSVRQDLSPLAVYMIKDKASSSGGTFLRWRSMQNARTGGTVWQPI
                     VDDKSVPVEVRKKVVAVEKDRILINMQISVFNHILRQLADCAEKLKEVDNAVAKSDLN
                     S"
     gene            9867..10319
                     /gene="ssb"
                     /locus_tag="PXE44_001735"
     CDS             9867..10319
                     /gene="ssb"
                     /locus_tag="PXE44_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5589229.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GERREVTEWHRIVFYRRYAEICGQYLRKGSKIYIEGKLRTRKWQDQNGQERYTTEIQG
                     ELLQMLDSRNATNSVDGQQSNYQAMSQSPKATHPQDLPMDNFDDDIPF"
BASE COUNT         3406 a         2038 c         2076 g         2930 t
ORIGIN      
        1 aaaataaaac cagaatataa tttgattgtt tttacaacaa taagcgtatt ttgatactat
       61 atctgttgta atttacatca cttagagcaa ttttgtatga ttttacttgt cattatgtca
      121 aattgatata ttatagcctc atcagacgcg gggtggagca gcttggtagc tcgtcgggct
      181 cataacccga aggtcgctgg ttcaaatccg gcccccgcaa ccacattcca aaccctcgat
      241 agtgaatcac atcgagggtt ttcgttttat ttgccaaaaa acagcaaaaa ataaccgctt
      301 gttgactgca aaagcttcct ctcgctttca tttctcgtta cacttctact acatttacgc
      361 ttttttgatt attatgagca atattttaac tgttacccaa ctcaactatt ccgtccgcca
      421 tttgctcgaa atggaaatga gccatatttg gctaaccggc gaaatctcaa atttcagcca
      481 accagtttcg gggcattggt atctcacttt aaaagatgac aaagcacaag tgcgttgtgc
      541 gatgtttaaa atgaaaaatg taagagtgaa ttttcgccca caaaacggta tgcaggtgct
      601 ggtgcgggca agtgttacac tctatgagcc acgaggtgac tatcagctca ttattgagag
      661 tatgcaaccg gcgggcgaag gcttgttaca gcagcaattt gagcaactta aagcaaaact
      721 ttcagctcaa ggcttgtttg ctcaagagca taaaaagcca attcctactt ttgttaaaac
      781 cgttggtatt attacctcct caagcggagc cgcattacaa gatattttac atattttgca
      841 acgacgagat ccgagcttat cagtggttat ttacccggct ctcgtgcaag gcaaagaagc
      901 aacgcaagat attgtgaata tgattgaact ggcaaaccgc cgcaatgagt gtgatgtatt
      961 aattgtcggg cgaggtggcg gttcattaga ggatttatgg tgtttcaacg aagagagtgt
     1021 cgcttatgcc atttatcatt cacaaatccc gattatcagt gcggtgggac acgaaaccga
     1081 tgtgaccatt gctgattttg ttgcagattt gcgcgcacca acgccttcgg ctgctgcgga
     1141 attagtcagc agagatcaaa atgaattgaa acgccaatta caacatcagt ttgataaagt
     1201 gaatttagcc tttgaccgag tttggaacca taaatttaac cgtttccaac aactcccctt
     1261 acgcctgaat gctcagcatc caagcaaaca aattcaatcg caacagcaac gttttgatta
     1321 tatggaatac cgcctcgagc aggcaattta tgcacttgtt gcagccaaaa agcaggtggg
     1381 gaaagagcta aatgtccgtt taattcacca acatccgcaa aagcagattg aacgccaatc
     1441 ccaaatgctt aagcaattgg agctgaaact ccattggcaa atggagcagc aactcagcaa
     1501 aaaacagcaa aaatggaaaa acattacgca acggtttgaa cgcaacccgc taccttatca
     1561 actttatcag caacaaacga tggtggcaaa aatgtcgcaa cgcttggctt attctatcga
     1621 aaaaataatg ctgaaagagc agcgacattt tcaagagtta tgcactaaac ttgatggact
     1681 tagcccattg aaaatcttga ccagaggcta ctcaattacc caaactaaag acggtaaaac
     1741 gcttacttca acagaaaata ttgaacgtgg tgaggctatt atcacccaac taaaaacagg
     1801 aaaaataata agtcaagtag tggatttttt gtaggggaaa ttttgaacgc tgccaatttt
     1861 taaaattatt tatagtaaaa tcagtaggtt aaatatttat aaacagggtt gttcacttag
     1921 ttatccacag gtgatgtgca taactatttt tgtaaagatg aattgagtgc tgatttttaa
     1981 atcaaccgat ttcaaaaggc atttttacgc ttgcacaagc ggtcatattt ccctataatc
     2041 gtgcaaaaat taagccagtg tggcgaaatc ggtagacgca gcggattcaa aatcttatgc
     2101 gtaaactgat aggacataca ggcgattgaa taaaaagcac tcttagcctg agtgatgaaa
     2161 ttggtagaca tgacggattc aaaatccgtt ggtgaataac cgtgtcggtt cgagtccgac
     2221 ctcaggcacc atttgaatca cacttgtagt ttctctcttt caaataatca attcaaatac
     2281 accgtattaa tggccaatac tccttaaccc ttgttaagag agtaaattat gaaaccatta
     2341 caaaacggta caaacaaacc ttttaccatt actattgctt caactaaagg tggctctgcc
     2401 aagagtacga atgctgctaa cattggtgca ttctgtgcag aacacggtct aagaaccctt
     2461 cttattgata ccgataccca acccacatta agctcttatt atgagttgga atatcaagct
     2521 cccggtggaa cttacgaatt tctgcatttt aaagatgtag atcctaccca cattatttct
     2581 agaacgacta tcccaaatct tgacttgatc cagtctaatg acccgtcaaa taaaatcagc
     2641 ccaatgctga gagattcacc tgacggtgca ttacgtttta gtttactgct ccagaaaatt
     2701 gaaggttacg atgtcatcat tattgacact cgtggtacca gagatattac tgtcgatatg
     2761 tctgtattgg cagcagatgt tttattctgt cctattcttc cccacatttt atctgctaag
     2821 gagtttattc gtggcacgat tggaatgtat caagacctcg aaacctttac ggcatttggc
     2881 tttaccctac caccgctgaa agcgatgatt aactgtgttg atcacactaa agatgtcaaa
     2941 ttggtttccg actaccttca tactctcttc caaaatgagt ttgatgaaaa caaaacattg
     3001 ctggaatttt cggtacctga ccgcgtggca tatcgtgaag cggcaacttt ttcaaaaccg
     3061 gtctatcgtc agagccaagc tgaatatgaa acgatcagtc agctttgtgc gttactgatg
     3121 ccacagtttg caaactctca cttctcagca tagagggaga atgtatgtct agcttaaata
     3181 aatcacttgt acagccgcat tctatagagg ctgaattttc agtaatcggt ggattactct
     3241 tggataatga actctttgat gaagtgagta gcaaaatcaa tgagggggat ttttataatt
     3301 ttactcaccg tctactgttt aagcatattt ccgaattgat tgaagccggt aaaccagcag
     3361 atatattgac attggatcaa taccttaccc agaaaaaagt gatcgaggat ttgggcggat
     3421 ttgcttatct tgcagaaata gtcaaaaaca ctccatctac agctaatatc cacgcttata
     3481 cggatatcgt tctcttatat agtaaacagc gacagctttt aaaactgggg cagtttattg
     3541 ttgatcagac acagagtgcg aaaacgccag ataagtttga agctgttgtt gatgacattg
     3601 agaaaaaaat tactgagttt agcctatcag ataatacaaa atctgcagca gatttaagtg
     3661 atatttttgc caatatgctc tctcgtatgg aactctcggc taaaaatgga gatcctgtga
     3721 caggtactcc aactggtatt caaggtattg acgaagcgac aacaggtgga cagccaggcg
     3781 atttaatcgt gattggtgcc cgcccttcta tgggaaaaac cgcattttct caaactatcg
     3841 cttaccatac acttaaaaaa ttcgagtcgg ctcctgtgtt ctatcacagc atggaaatgc
     3901 cggcagatca gattttacaa cgttttctag ctatgcgtgc cagagtggga ttgcaaaata
     3961 ttagacaggc agacagtttg gatgatgagg aatggtcaaa actaacctca gcaatgggct
     4021 atatcctaga acattggaaa aatcgtttag ttattgatga tgaaggtagt ctcacgcctc
     4081 aaaaattacg cactaaagtt cgacaaaatg ctcgtaaata tggcaaaccg gcagctattt
     4141 tcattgatta cattcagtta atgcaaacct cgcaacgtta tgaaaatcgc aatttagaaa
     4201 ttgccaacat ctctcgttca ctcaaattac tggcaaaaga gatggattgt ccggtttatg
     4261 ccctctctca gctgaaccgg tctgtggaac aacgtgccaa taaacgcccg atcaactctg
     4321 atttacggga atccggctca cttgagcaag atgccgatgt cattttattc atctaccgag
     4381 atgaagttta tgatgcggat tctccgttta aaggcactgc tgaaatcatt atcggcaagc
     4441 aaagaaacgg accacttact tccgtaatga cgaatttcag aagtgaattt gccctatttg
     4501 aaaacagaac aaacacaacg cattaggata gattatgaca aaagatgaaa aaagactggc
     4561 agcgatagca aaaggattaa atgtttcacc tatttcaaat acggctccat cctaccaaac
     4621 agccacacca acgcattaca gtagcgatgt tgaatatatt acagtaacgc tggataagtt
     4681 gcgtccgtat gagcataacc ctcggaaaac ccgtaaccct aactttgaga tgatcaaaga
     4741 gtctattcgt cgtcgagggt tagatcataa gccgaatatc acccgtcgtc cgggagaaga
     4801 tttctatatt atcgctgacg gtggtaatac tcgtattcag gcactcaagg agctctttac
     4861 cgagaccaaa gatccaaaat tttggtccat cagttgtcaa tataaacctt ggcaaggtga
     4921 tactgccgac agtgtcgaag ctgaactcaa tttacttatc gggcatttga ttgaaaatga
     4981 tacccgtgcg gatttgtcat ttattgaaaa agcattgggg attttgcagg cgaaggagta
     5041 ttacgagaaa aaattaggta agtctctttc ttcgagagag ttatcttctg agttagaaaa
     5101 tgatggatat attatctctc atgctctcat tcttaaaatg gaacgttgtg ttaattactt
     5161 atatccacat ataccagaag ttctttttag tggtcttggt catacacaga tcgataaact
     5221 attagctatt cgtaataatg ctgaagaggt atgggcagcc catcaatttg atactgatgt
     5281 tacttttgaa tcgctctggt cagaaaattt atccaaattt aatgaggcta caccatttca
     5341 agcaaaagaa ttccaaagtg aattgattac ggcaatggtt gaagctttag agggtaaagt
     5401 gagttttgaa acactttatc tggatattga cttagatgaa caaaaattca aaaaaatagc
     5461 ggctaaacaa caagaaattg ataatttagt tgaaaaaaca gtcgatcaaa tcaataccag
     5521 acaggaacaa caaactgcaa aatctgaacc tctcatcaaa aatgaaaata aagccgttac
     5581 tccccaaaaa gctcaacata ttattgatga ggtaattgat gataacgaaa gtgatgatga
     5641 gcagaataac gctattactg atttatctca gcttggctct tcattgctcg ataaccaaga
     5701 taatcaagaa gattatctat cccaaattgc ccataacttt ggactcactc cgggaatgag
     5761 tattcaagaa cagcgagagc aacgagctga agcgaatgga ctgacttttg cttgtgaagg
     5821 tcgccaaccg gttgaagata tttggttgat tcaccccgca cgtcaatatc gctcagaggc
     5881 ctactcactg gccttagata ttgccgaaag tatgggcatt gaccatctgg ttgaacatat
     5941 tgtgaaacaa ccagtggatt acacttatgc aatgaaaccg gcagatactg aactttcacc
     6001 gtctgcttta acactctact cattcctctc aatgttacag actgctgaac ataaagatga
     6061 aaatagctgt gtcttagatt ccaaattcct gtttgagctt gatgatctta ccttagtcaa
     6121 aattttccgt ctcattcggg tcagtcgcca tattcgccaa caaggaggag aaaatgatta
     6181 agccgattac cgtaaatgaa gcattgattg gtaacgccct gctcaatatt aaagagggaa
     6241 atatccatcc ttgtctaaaa atgggatttt ctgaggagca actaaaagcg atcaataatc
     6301 tcagtattga tgaaattatg gatattagca actccgttgt ctcttttgct aaggtggaga
     6361 ttaatcacga aaccttttgg aaattactgg ctattgcaca agccaatact caacagcgac
     6421 aaattattga ccgagcatta ttgctcggag cctctattga aatgttgcac cagtattttg
     6481 gtttatcaac ctctgaagtc tcggctcgcc gtcaattact cggtatcgaa gaaaaaatgg
     6541 gacgaaaagc ggcagcaagt gatgaagaat ctacccatat ttgggaaatt tggcaaagat
     6601 acaaacaacg aatggaaatc ctagattcac aagaaggatt agagcttcta tgtctgattg
     6661 ctgaggaagg caatatgaat ctaacggttg tttggaaact ggtttccgaa tggtattcaa
     6721 aacataagta aaaaataaat gccccgaagt tggagctttg gggcattcga tattgagtct
     6781 tgtatctcta tcttatgaat tacaaggact ttgcagaagt cccgtgcatt atagccatag
     6841 gagagagaat agcaagagaa aaactctttt atcctggtgg agagaaacaa tgtttagcag
     6901 cacgaataag ccaacagagc atggattgct attttttggc aaccaacacg aaacagtccc
     6961 tactcgatta ttacaagatc cctgccttac accaagagca aagtttgctt ggcagctcat
     7021 taagtgcaat gttcaagcct ttcaaggagg attatttccc tcttatgaga cgctcagcaa
     7081 aatgttatcc gatagacctt atgcggaggc taaattatcg gaaaaagtcg tgactcaaac
     7141 actctattta ctccgtctta ctcgctggct tactttatgt gaaacagtgc gtgatgagcg
     7201 aggttatgta caaggtaatg tttatatcct acatgatgaa cctgtgccaa ttttagatgc
     7261 aattcaatta aatagcgact atctctcttt tttagataaa tgcaccaaac ataaagatag
     7321 ccttattaat ggtgtggcaa atcatattgt tgaaagtcta ataacagaca aaaccaaatg
     7381 gcactatgtc tctcatttag actgtttaca aagtcgttat cagatatacc agcaacagca
     7441 gctggtgaaa gataaacagc catcgcaaac agaacaagaa ttaagcaaaa ttcaacaaaa
     7501 catcctaact tccactatgg aagttaggga ttttgatgag gaagtcagga aaaaatcctc
     7561 taaaaaccag acttccaata agggagttag ggctaaaaat gaagataagt cattgatttt
     7621 agacttactt ccattaggga agtcagatgt acagtaccgt actttatata ttaataagta
     7681 ctgtactggg gaaccgagcg aaattacttg gccacaagaa atcccattaa cttcacttga
     7741 acaaaaactt actgcacagg caatgaaggg attagacctc ggtttatgcc aagctatttt
     7801 gttggaagta caacaacgca ttattcaaaa gcaggatgtg aaaaaaccga aaggttatct
     7861 ctatacgctt atccagcggg cgaagcaggg tgaatttaaa ccctactatt ttgaattagg
     7921 aaaaaatctt ccctcacaga atcaggctaa caataattct gctcattcta tacaaaaact
     7981 gactgagcag cctactgaga aaccggtctt aacttcagca gacagagagc aaatggctag
     8041 tcgtatgcag aagtttagag ctgaattact tttacaataa gattaaaaaa caatcactgt
     8101 taaggggctt aatagtgagt gaaaaataat cagtgtgtac caggtacaca atggataaaa
     8161 aataaacagc gtaggcagtg tctgcaaaga ttaaaaaaca gacaatggaa agcccctttc
     8221 caaagactaa aaaataatca aataaacaag gagcatatat gaccactcaa ttagatcctc
     8281 aacttggtcc acttcgtagt gagatcactt ttacgttaca tacccaatat gcccataaat
     8341 tatgggtggg gcgaccaatg attcgtaatg aggaaggcaa agtaactcaa tcctccatta
     8401 tcagcgtacc aaattgcttt gcaatgctaa cccaaattca acgggctgca tcagaagatg
     8461 atccttatgc tgacgattat ctgattcaat ttgaagaaag tgtgatcaat tatcggaagg
     8521 aaatccaaaa gctcaccaac gacattgtca cgctctacgc caaaatgttg ccggagggaa
     8581 tttctattga acgctgtgcc aatatttccc cgatttctta cccgatttat gttaattcac
     8641 agctagggta tcaattactc tacttacttg gtgattttga tagcctttgc cgttcagtaa
     8701 tgacggcagc tcatattgcg attatcaatc gtgcagaagc tcaagattgg attgaagctg
     8761 gtgcaaggtt aatccggaaa tgttttggga tcgttgagcg ttataagagt tcaggaatca
     8821 ctcgtcggga ctatcaagaa aagaatgctc gctatcaagc tgcgataaaa cgaatgggct
     8881 atacactctc agatgctgta cttaccggtg agcatcgtgc tgagtttgct cccttcatca
     8941 agcaaaacac tactgttgaa gaagagcaat ccgtagaaac ccatatcacg gcacagacaa
     9001 atgaatctca aaaataagga gtgatatgaa cctcatctct gatgaaattt atcggcaatt
     9061 agtcaaagat tctgggctga atacttctct gaaacagcta tttagccatt ttgacactgg
     9121 ctcagactat gaacttttgc aagaacagtt cactcaagct cgggcttatt ttatggctgc
     9181 acaggatagt atggtgcagt ctgtgcggca ggatctttca cctttggctg tttatatgat
     9241 taaggataag gcctcatcct caggcggtac atttttacgc tggcgtagta tgcaaaatgc
     9301 cagaaccggt ggcacagttt ggcagccgat tgtagatgat aagtctgtcc cagtggaggt
     9361 gaggaaaaaa gttgtcgcag tagaaaaaga caggattttg attaatatgc agatttcggt
     9421 gtttaatcat attttgcgac agttggcaga ttgtgctgaa aagttgaaag aggtagataa
     9481 tgccgttgcg aaatcagact taaattcgta gtaaatttat atataacaac tcctatattg
     9541 gttgtgtgta aacgccaacc acggtgggct gacgtggtaa aacaaacagt ccgttaatta
     9601 ggacatattc aagtttgtaa tacctgaaaa tccggtttgt aatccctgaa aaagtgcagt
     9661 ttgtaaatcc tgcattaaaa ggccttttaa aggtaagggt acaagcccct taccttcacg
     9721 aagaaaaagc ctttacaacg gaggaataag tgattgcaca cgtaaaaatg aaaaattcac
     9781 tctaaccaaa ttttaattaa atactttttt cttaggctga atcaagttta atagaactaa
     9841 cttaatttat tgaaggagta aatacgatgg caggaatcaa taaagtgatt attgtcggta
     9901 atcttggcaa cgatcctgaa atgcggacta tgcctaatgg tgaagcagtt gctaatatta
     9961 gtgtggcaac ttctgaaagt tggacggata aaaataccgg agaacgtcga gaagtcactg
    10021 aatggcatcg tatcgttttt tatcgccgtt atgctgaaat ttgtggtcag tacctacgta
    10081 aaggctctaa aatttatatc gaaggaaaat tacggacacg taagtggcaa gaccaaaacg
    10141 ggcaagagcg ttatacaacc gaaatccaag gtgaactatt acaaatgtta gatagccgta
    10201 atgctacaaa tagcgtagat ggacaacaat ctaactatca agctatgagc caatctccaa
    10261 aagcaacaca tccacaagat ttaccaatgg ataactttga tgatgatatt ccgttctgag
    10321 ttacatataa tttaaaactg taaaataaaa cccatataaa tatagagaac cctttatttt
    10381 aaaagggttc tcttttttta cattaatttc gacacaacat ctttaatttg atagtattta
    10441 tatatgatat
//
LOCUS       DANLZC010000090        10384 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.090, whole genome shotgun sequence.
ACCESSION   DANLZC010000090 DANLZC010000000
VERSION     DANLZC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.090"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..1861
                     /locus_tag="PXE44_001736"
     CDS             <1..1861
                     /locus_tag="PXE44_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDL5589230.1"
                     /translation="QKLHQINNRLKIVATDSQWNTPLAIFWTLVLCLPSTLMFLAGFV
                     AITYISFENPQEVWTWGLKMAIYWLYFAFLVAMLRPHGIGHIHFNMPQKSNEIFRNIL
                     KRSVWIIALLLNTSIFTNLEMGISYDMLGQTMTIFVLILMIWMIVPAFRQAISTYENV
                     TTEEKKESKSILLTIVKLVLLLAPIILIILIALGYYYTALVIIEHLVDSYFAIITWII
                     IRNILYRAFSISSRRLAYRRLQEKREAAEAKTRAKAEQNDENPDGVELPFELKEDSIA
                     VSEVKNQMLKLTDFVLWIGLFVLLYWVWSDLITVALYLDGVTLWQQSVTTEAGIVMES
                     ITLLNLLTAILIIVVMVVLIRNLSGLLEALIFSHFKLSQGTPYTITTLLTYLIIALGA
                     IFAFGTLGMSWSKLQWLLTALSVGLGFGMQEIFANFVSGIIILFERPMRIGDTITIGT
                     YSGTVSKIRIRATTLVDFDGKEVIVPNKAFVTERLVNWALSSTATRIIVQVGVAYGSD
                     LELVKRLLLQVAEENPKVLKDPQPRAYFLTFGASTLDHELRAYVNDLSDRNPTIDALN
                     TRIHQLFNEHNIDIAFNQLDVYIKNNDTQDEIKVDSKRFEFERPNNITAKEKE"
     gene            1864..2946
                     /gene="aroC"
                     /locus_tag="PXE44_001737"
     CDS             1864..2946
                     /gene="aroC"
                     /locus_tag="PXE44_001737"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL5589231.1"
                     /translation="MAGNSIGQLFKVTTFGESHGIALGCIVDGVPPNMALSEADLQPD
                     LDRRKPGTSRYTTPRREDDEVQILSGVFEGKTTGTSIGLIIKNGDQRSQDYGDIAEKF
                     RPGHADYTYQQKYGIRDYRGGGRSSARETAMRVAAGAIAKKYLREQFGIEVRGYLSQI
                     GNIKIDPKTVENIEQIDWHAVNSNPFFCPDPVAIEGFDALIRELKKEGDSVGAKLTVI
                     AERVPVGLGEPVFDRLDADLAHALMSINAVKAVEIGDGFAVVEQRGSQHRDEMTPQGF
                     LSNHAGGILGGISSGQPIIAHIALKPTSSITIPGRSVNKNNEAVEVITKGRHDPCVGI
                     RAVPIAEAMMAIVLLDHLLRFKAQCR"
     gene            2965..3792
                     /gene="mepA"
                     /locus_tag="PXE44_001738"
     CDS             2965..3792
                     /gene="mepA"
                     /locus_tag="PXE44_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDL5589232.1"
                     /translation="MKLLKTLLVSSLAFTATALNATQWEKIKTPVQGKAQSIGGYSNG
                     CIIGAQPLALKGEGYQVIRSARNRYYGHPQLLDYLTHLGKKTKAAGVPTILIGDMGMP
                     AGGRFASGHASHQTGLDADIWFKFGPMDDETARNPAGLATIMVNRSTERVDDSLWNQH
                     HTNLVKLAATDPRVDRIFVNPAIKVKLCQTAGNDRAWLRKIRPWYGHDSHFHVRLTCP
                     ADAASCENQALVPAGDGCGEELYSWFEPKEASGTPKAKVLPTAPVQCQMILSEQGLN"
     gene            3865..5784
                     /locus_tag="PXE44_001739"
     CDS             3865..5784
                     /locus_tag="PXE44_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL5589233.1"
                     /translation="MRLFVAEKPSLARAIADVLPKPHTKGDGFIQCGEHDTVTWCIGH
                     LLEQAEPDVYDERFKMWRMEHLPIVPEQWKLIPKKETLKQFKVVEKLIKKADVLVNAG
                     DPDREGQLLVDEVFGYLNLSAERKSQIQRCLVSDLNPNAVSRAIEKLQKNSDFIPLAT
                     SALARARADWLYGINMTRAYTLQGRWAGYKGVLSVGRVQTPVLGLIVRRDLEIEHFVP
                     KDYFEVLAQIVVPETQERFKAQWQPSKACEDYQDEDGRVLSRPLAENVVKRITDQPAT
                     VTEYQNKIEKETAPLPYSLSVLQMEAAGRYGLSAQAVLDICQKLYETHKLITYPRSDN
                     RYLPTEHFNDRFKVMAAIAHHLPEYQQKPEVIDATIRNRCWNDKEVEAHHAIIPTARQ
                     GTVHLTENEKRVYQLIARQYLMQFCPDAEYRKSKIGLEIAGGSFVAQARNLVIAGWRA
                     LLGKEDSLDDAEPLLPIVKKGQILHCEKGEIVSKKTQPPRHFTDRSLLSAMTGIARFV
                     QDKELKKILRETDGLGTEATRAGIIELLFKRGFLIRKGKNIHSTEAGRILIQALPDSA
                     TQPDMTAHWEMQLNQISKKETSYQQFMFDLNQQLPHLLQSPNRQVLQNLAKITPLASG
                     NKPRYFKKRTQTAEN"
     gene            5890..6216
                     /gene="secG"
                     /locus_tag="PXE44_001740"
     CDS             5890..6216
                     /gene="secG"
                     /locus_tag="PXE44_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL5589234.1"
                     /translation="MLINVLTIVYLVVAVLLIAFVLMQQGKGADAGASFGAGASATVF
                     GSAGSANFLSRTTALLATIFFAISLVIGNLSSHNVAPKSQFEDLSQVEQKQVEPVKIE
                     NSDIPQ"
     gene            6241..6326
                     /locus_tag="PXE44_001741"
     tRNA            6241..6326
                     /locus_tag="PXE44_001741"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6275..6277,aa:Leu,seq:gag)
     gene            6529..7770
                     /locus_tag="PXE44_001742"
     CDS             6529..7770
                     /locus_tag="PXE44_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5589235.1"
                     /translation="MARTTKALNKTQIENAKSKDKEYILSDGDGLNLRVKPLPKGSKI
                     WIFNYTNPQTKNRTNLTIGRYPLITLAEARTICDGYRSLIEKGIDPQEEKKRLQQEAE
                     NRVNDTFRNVAESYFNGIYKEKAKNPITREKNWIRLENHIFPYIGDKHVEEIKVKALV
                     DIYSKVADKSNTLKKLHQLVSAVMDHAITQGIIESHNCRLAVKNFYIKPSTPNPTIEL
                     EELPQLFTDLHNAKLSKQTYALVCWSFLTALRPNEAVNAEWKEIDFEKKLWNIPKEKM
                     KGKADKKRPHSVPLSSQALKILEMMKMHSNSSPFVFPGRSSNSKPMSNATVNSTLKRN
                     GYKDRLTAHGIRAFVKTFLSSRKVDRHVSESILSHLLEGGDDLENTYNRYDFLEERVP
                     VMQLLGDYCEQCGMNLELVRG"
     gene            complement(7929..9797)
                     /locus_tag="PXE44_001743"
     CDS             complement(7929..9797)
                     /locus_tag="PXE44_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL5589236.1"
                     /translation="MFKKLLELRQQKAAIEAEMRSILTKADEEKRSTTEAEGAKFDEL
                     RSQAENLVKEIARYEAITDEQRNQPGQQVETANVKVSDDELRHWIKTGELRSLATNTN
                     GGADGGYSVIPELDKQVMKRLTDDSVMRQIANVVKLTQGKEYKKLVSAGGAAVNHIGE
                     GEARTETATPKMNEVTIAVHNIYAYPKTTQEILDFGGVDILGWLTEEISESFVETEET
                     DLTNGTGTKQAKGFLAYPRTTANDKTRPFGTLQKMEVQPATVIGWNSKGQPETLTPYP
                     PYSVNVQRTVNRQNWLTAYIPHSYDENVAETPHFDQWLNFVSDGKQDKAKNILAALYA
                     ILTNRYNWQVFFQVTGKGGSGKSVFAGIATLLAGEKNTASARLENFDDERKIAGLEDK
                     KLIICSEQTKYAGDGGGLKAITGGDMVRVDPKNKHPFNARIAAMIMIVNNDPCKWTER
                     NGGIDRRIVNFRFNKRPPENERDPYFMDKITLEIGGIIRKVLDTFPDPLEAKRALEEQ
                     KESLEALEIKKQSDPLTDFFEYLYTTENTDGLYIGTGNTLGHDKIRTHLYPAYLAFVK
                     AKNVLELGLNTFVVGIEQAVKQHGNKHDFTKRKTNKGQRTNVHFKNFDDFQSDILN"
     gene            complement(9808..10203)
                     /locus_tag="PXE44_001744"
     CDS             complement(9808..10203)
                     /locus_tag="PXE44_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5589237.1"
                     /translation="MISNEAIRQEVIDLLQGNIQGIQHFHNGRAVFTDVENELPAIAV
                     FIDEAEYSQLTLCDGECNAYLKIGIYLPMFSTENDLDLVAQKISDVMNQATFHSMDEC
                     VLAKYSYDYDANESAWKTATLHYNINYLN"
BASE COUNT         3053 a         2197 c         2175 g         2959 t
ORIGIN      
        1 acaaaaatta caccaaatta ataatcgact aaaaattgtc gccacagata gccaatggaa
       61 cacccctctt gctatttttt ggacattagt gctttgcctg ccaagtacac taatgttctt
      121 agccggattt gttgctatca cttatatttc atttgaaaat cctcaggaag tctggacttg
      181 gggattaaaa atggcaattt attggctgta ttttgccttt ttagtggcga tgctacgccc
      241 gcacggcata ggacatattc actttaatat gccacaaaaa agtaatgaaa tcttccgtaa
      301 tattttaaaa cgttcggtat ggatcattgc tttattactg aacacctcta tttttacaaa
      361 cttagagatg ggaatatctt acgacatgct tggacaaaca atgactattt ttgtccttat
      421 attaatgatt tggatgattg taccggcatt taggcaagct atttctacct atgagaatgt
      481 cacaacagaa gagaaaaaag aatctaaaag tattttactc actattgtca aattagtgct
      541 actgcttgcc cctatcattc ttatcattct gattgcattg ggctattact atactgcact
      601 ggtgatcatt gagcatttag tagattctta ttttgcgatc attacttgga ttattattcg
      661 taatattctc tatcgagcat tctctatttc atcacgccgt ctggcttaca gacgtttaca
      721 agaaaaaaga gaagcagctg aagccaaaac acgagctaaa gcagaacaaa atgatgaaaa
      781 cccggacggt gtcgagcttc cttttgagct aaaagaagac tctattgccg taagcgaagt
      841 caagaaccaa atgttgaaat taactgattt tgtgctttgg attggcttat ttgtgctgct
      901 ttactgggtt tggtcggact taattactgt tgcactctac ctagacggcg ttaccttatg
      961 gcaacaaagt gttacaacag aagccgggat tgtaatggaa tccattaccc tgctgaattt
     1021 actcactgct attctgatta ttgtggtaat ggtggtactc attcggaact tatccggttt
     1081 acttgaagcc ctgattttct cgcacttcaa gttatcgcaa ggtacaccat acacaattac
     1141 cacactctta acctatttga ttattgcgtt aggggcaatc tttgcgttcg gtacattagg
     1201 gatgtcttgg agcaagctcc aatggctact aactgcactt tctgtcggtc tgggttttgg
     1261 tatgcaagag atctttgcaa acttcgtttc agggattatc atcttgtttg aacgtccaat
     1321 gcggattggc gacaccatta ccatcggcac ttacagcggt acggtttcca aaattcgtat
     1381 tcgtgccaca accttagtcg attttgacgg caaagaagtg attgtaccaa acaaagcatt
     1441 cgtaaccgaa cgcttggtga actgggcatt aagcagcacc gcaacccgca ttattgttca
     1501 agtcggcgtg gcttacgggt ctgatttaga attagtcaaa cggctgttat tacaagtggc
     1561 ggaagaaaat ccgaaagtct tgaaagatcc acaaccaagg gcttatttcc ttacctttgg
     1621 tgcaagcact ttagatcacg aattaagagc ctatgtgaac gatttatctg atcgtaaccc
     1681 aactattgat gcactaaata cacgcatcca ccagctcttt aatgaacata atattgatat
     1741 tgcatttaac caattagatg tttatatcaa aaacaacgat acacaagatg aaattaaagt
     1801 agattcaaaa cgctttgagt ttgaacgacc aaataacata acagcaaaag aaaaggaata
     1861 acaatggcag gcaacagcat cggacaatta tttaaagtga ccactttcgg cgaatctcac
     1921 ggtattgcat taggctgtat cgtagatggc gtaccgccga atatggcttt aagcgaagca
     1981 gatttacagc cggatttaga ccgccgtaaa cccggtactt cacgctacac cacgcctcgc
     2041 cgtgaagatg atgaagtgca aattttatcg ggagtctttg aaggcaaaac gacgggaaca
     2101 agcattggtt taatcattaa aaacggtgac caacgctcac aagactatgg cgacattgcc
     2161 gaaaaattcc gcccggggca cgcagattat acctatcagc aaaaatacgg cattcgagat
     2221 taccgtggcg gtgggcgttc ttctgctcgg gaaaccgcaa tgcgggttgc cgccggggcg
     2281 attgccaaaa aatatttgcg tgagcaattt ggcattgaag tgcgaggcta cctctcacaa
     2341 atcggcaata tcaaaattga cccgaaaacc gttgaaaata ttgaacaaat tgattggcac
     2401 gcagtcaata gtaatccttt cttctgcccc gatcccgttg cgattgaagg ctttgatgcc
     2461 ttaattcgtg agctgaaaaa agagggcgat tcggtggggg caaaattaac cgtgattgca
     2521 gagcgtgtac ctgtagggtt aggcgaacct gtgtttgacc gcctagatgc cgaccttgcc
     2581 cacgccttaa tgtcgatcaa cgccgtgaaa gcggtggaaa ttggcgatgg ttttgcagtt
     2641 gtggaacaac gtggtagcca gcaccgagat gagatgacgc cgcaaggctt tctctctaat
     2701 cacgccggcg gaattttagg tggtattagc tccggtcagc cgattatcgc tcatattgcg
     2761 ttaaagccga cctcaagcat tactattccc ggcagaagcg tgaacaaaaa caacgaagcc
     2821 gttgaagtga tcactaaagg cagacacgac ccctgtgtag gtattcgtgc tgtgccgatt
     2881 gcagaagcga tgatggcgat tgtactactc gaccatctgt taagatttaa ggctcaatgc
     2941 cgttaaacac ataaaattgg aaaaatgaaa ttattaaaaa cacttttagt ctcaagcctt
     3001 gcttttacag ctaccgcact gaatgcaacg caatgggaga aaatcaaaac tccggttcaa
     3061 ggaaaagcac aatctatcgg cggttatagt aacggctgta ttattggggc acagcccctt
     3121 gcattaaaag gcgaaggcta ccaagtgatc cgctcggctc gtaaccgcta ttacggacac
     3181 ccacaactgc tcgattatct cactcactta ggcaaaaaaa ccaaagctgc cggtgtgccg
     3241 accattttaa tcggcgatat gggaatgcct gccggtgggc gttttgcctc cggtcacgcc
     3301 tcccatcaaa ccgggctgga tgctgatatt tggtttaagt tcggaccgat ggacgatgaa
     3361 accgcccgca atccagcagg gcttgccacc attatggtaa accgtagtac cgagcgggtt
     3421 gatgattcac tctggaatca acaccatact aatttagtaa aacttgctgc aaccgatcct
     3481 cgagtggatc gcatttttgt caatcctgcc attaaagtga aactctgcca aaccgcaggc
     3541 aatgatcgtg cttggttacg caaaattcgc ccgtggtacg gacacgactc ccatttccac
     3601 gtccgtttaa cctgcccggc tgatgccgca agttgtgaaa atcaagccct tgtgcctgcc
     3661 ggtgacggct gtggtgaaga gctttactcg tggtttgagc cgaaagaagc ctccggcaca
     3721 ccaaaagcca aagtgctacc cacagcgcct gttcaatgtc agatgatttt atctgaacaa
     3781 ggcttgaatt aattgcaaaa taacaacctt ttttgaccgc ttgtcagccc acaagcggtt
     3841 cattttctta aataatttac gattatgcgt ttatttgttg ccgaaaaacc cagccttgcc
     3901 cgagcgattg ccgatgtgtt accaaaaccc cataccaaag gagatggctt tatccaatgc
     3961 ggcgaacacg acactgttac ttggtgtatc ggtcatttat tggaacaggc tgaacccgat
     4021 gtctatgatg agcgtttcaa aatgtggcga atggaacatt tgccgattgt gcctgaacaa
     4081 tggaaattga ttcccaaaaa agagacgctc aagcagttta aagtggtgga aaagctgatt
     4141 aaaaaagccg atgttttagt caatgccgga gacccggatc gagaagggca gctgttggtg
     4201 gacgaagttt ttggctattt gaatttatct gctgaacgca aaagccaaat tcagcgttgt
     4261 ttggtaagcg atctcaaccc gaatgcggtg agccgagcca ttgaaaaatt gcaaaaaaac
     4321 agcgatttta taccgcttgc aacctctgct ctcgcccgag ccagagcgga ttggctgtat
     4381 ggtatcaata tgacccgagc ctatacccta caggggcgtt gggcaggcta taaaggcgtg
     4441 ttatcagtcg gacgtgtgca aacgccggta ttagggctga ttgttcgccg agatctggag
     4501 atcgagcatt tcgtgccaaa ggattatttt gaggtgttgg cacagattgt cgtgccggaa
     4561 acccaagagc gatttaaggc gcagtggcaa ccgagcaaag cctgtgaaga ttatcaagat
     4621 gaagatgggc gggtgctgtc tcgtccattg gcagaaaatg tggtaaaacg gattaccgac
     4681 cagccggcaa ccgtcaccga atatcaaaat aaaattgaaa aagaaactgc accgctgcct
     4741 tattctcttt cggtattaca aatggaagcc gccggcagat atgggctatc cgcccaagcg
     4801 gtgttggata tttgccaaaa gctgtatgaa acccataaac taattaccta ccctcgttct
     4861 gataatcggt atttgccgac cgaacatttc aacgacagat ttaaagtgat ggcggcgatt
     4921 gctcatcatt tacctgaata tcaacaaaaa ccggaagtga ttgatgccac tattcgcaat
     4981 cgttgctgga atgataaaga ggtggaagcc caccacgcca ttattcccac tgcacggcaa
     5041 gggacggtac atttaaccga aaatgaaaaa agagtttatc aactcattgc ccgccaatat
     5101 ctaatgcaat tctgccctga tgcggaatat cgcaaaagta aaatcgggct tgaaattgca
     5161 ggtggtagtt ttgtcgctca agcacgcaac cttgtaattg ccggctggcg agcgttactg
     5221 ggtaaagaag atagccttga cgatgcagag ccactactac cgattgtcaa aaaagggcaa
     5281 atcctccatt gtgaaaaagg ggaaattgtc agcaaaaaaa cacaaccgcc ccgccacttt
     5341 actgatcgca gtttgctttc agcaatgacc gggatcgccc gttttgtaca agataaagaa
     5401 ttgaaaaaaa tcttgcgtga aaccgatggt ttaggcacgg aggcaacccg agccggtatt
     5461 atcgaattgc tgtttaaacg tggtttttta atccgtaaag ggaaaaatat ccacagtacc
     5521 gaagccggca gaatattgat tcaagcactt cccgactctg ctacccagcc ggatatgacc
     5581 gctcattggg aaatgcagct gaatcaaatc agcaaaaaag agaccagcta tcagcaattt
     5641 atgtttgatc taaaccaaca gctcccacat ttattacaaa gcccaaaccg ccaagtgttg
     5701 caaaatttag cgaaaatcac accgcttgca agcggcaata aacctcgtta ttttaaaaag
     5761 cgaacacaaa ctgctgaaaa ttaaatcaag caggtaaaaa ttactgttta gcacttgtca
     5821 aacgggcgta aattctatat tatgcacgcc attgcaaatt tcgcataaag aatacaagag
     5881 aataaattaa tgttaattaa tgtactcact attgtttatc ttgtagttgc cgtattactg
     5941 atcgcatttg tattaatgca acaaggtaaa ggcgctgatg caggtgcttc tttcggggcg
     6001 ggtgcttcag caacagtttt cggctcagca ggatcagcaa attttttatc aagaacaact
     6061 gctctacttg caacgatttt cttcgcaatt agtttagtta tcggtaattt aagctcacac
     6121 aatgtagcac cgaaaagcca atttgaagac ttaagccaag ttgaacaaaa gcaagtagaa
     6181 cctgtaaaaa tagaaaatag cgatatccct caataatcgc ttaacattaa aaagaataaa
     6241 gctctggtgg tggaattggt agacacgcca tcttgagggg gtggtggaca cagtccgtgc
     6301 gagttcaagt ctcgcccaga gcaccattca actaaatttg gctcaacgga gctaaactaa
     6361 aaaagcctta gaaagcctgt aaaatcaaca tttacaggct tttttactat cttaactcaa
     6421 cggctctaaa cgtttcctat cacaaatttt agttacacgt ttagttacac gttttaaatc
     6481 gtgttactat actttcaata atcgtgtaac acttcgtagg tttaatttat ggctagaaca
     6541 actaaagcat taaataaaac tcaaatagaa aatgccaaat caaaggataa agaatatatt
     6601 ttatccgatg gcgatggatt aaatcttaga gttaaacctc tccctaaagg ctctaaaatt
     6661 tggattttta actacactaa cccacaaact aaaaacagaa caaatttaac tattgggcgt
     6721 taccccttaa ttacacttgc ggaagctcgc actatatgtg atggctaccg ctcgttaata
     6781 gagaaaggca tagatccaca ggaagaaaaa aagcggctgc aacaagaagc agaaaatcga
     6841 gtaaatgata ctttccggaa tgtggccgaa agctatttca atggtattta taaagagaaa
     6901 gcaaaaaatc cgattactcg tgaaaagaat tggataaggt tagaaaatca tattttcccc
     6961 tatatcgggg ataaacacgt tgaagaaatc aaagtaaaag cccttgttga tatttattct
     7021 aaagtggcag ataaaagcaa cacattaaaa aaactgcatc agcttgtatc tgctgtaatg
     7081 gatcacgcca ttacgcaagg cattatagaa agccataact gccgattagc agttaaaaac
     7141 ttttatatca agccatctac gccaaatccc actattgagc ttgaagaatt accgcagtta
     7201 tttactgatt tacataatgc gaagttatca aagcaaactt acgcccttgt ttgttggtcg
     7261 ttcttgactg ctctacgccc aaatgaagcc gttaatgctg aatggaaaga aatagacttt
     7321 gagaaaaagc tatggaatat ccctaaagag aagatgaaag gaaaagcaga taaaaaacgc
     7381 cctcactctg tgccgttatc ctcccaagcg ttaaagatat tagagatgat gaaaatgcac
     7441 tctaattcta gcccttttgt ttttccaggt cgttcatcaa atagtaaacc aatgagcaac
     7501 gcaacggtaa attcaacatt aaaaaggaat ggatataaag atagactaac cgcacacggt
     7561 attcgggcat ttgtgaaaac atttctatct tctcgcaaag tagatcgcca cgtttccgaa
     7621 agtatattat cccatttatt agaaggtgga gatgatttag aaaacacata caaccgatat
     7681 gattttttag aagagcgtgt gccagtaatg cagcttttag gtgattattg cgaacagtgc
     7741 ggaatgaatt tagagttagt tcggggttag ggaggtaaaa tatatgattt tagtcgtcac
     7801 gtcatcactt ttcccacttt cccctttaaa atcaaatagt taagtggtga tgactggttt
     7861 taaagtcatc attaagtcat cacaaaaagg tgatgaccta gaaaaaaaga ggggtttccc
     7921 cctctttgct aattcaaaat atcactttga aagtcatcaa aatttttgaa atgaacgttt
     7981 gttcgttgcc ctttattcgt ctttcgtttt gtgaaatcgt gcttattgcc gtgttgcttt
     8041 actgcttgct caatacctac tacaaaagta tttaagccta gttctagcac attcttagcc
     8101 ttaacaaagg ctaaatatgc cggataaagg tgcgttctta ttttgtcgtg tccaagcgtg
     8161 ttgccagttc cgatataaag cccgtctgta ttttctgttg tatatagata ctcgaaaaaa
     8221 tccgttaatg ggtcagactg ctttttaatc tccaacgcct ctaggctctc tttttgctcc
     8281 tctaaggctc ttttagcttc gagtgggtca gggaaggtat caagcacttt acggataatg
     8341 ccccctatct ctaatgtaat tttatccata aaatacggat cacgttcatt ttcaggcggt
     8401 cttttattaa aacggaaatt gacaatccgc ctatctatac cgccattccg ttctgtccat
     8461 ttacaagggt cgttattgac gatcataatc attgccgcaa ttcttgcgtt aaatgggtgc
     8521 ttgttcttcg gatcgactcg caccatatca ccgccagtaa tcgctttcaa tccgccacca
     8581 tctccagcgt atttcgtttg ctcggaacag ataatcagct ttttatcttc cagccctgcg
     8641 attttgcgtt catcatcaaa gttttctagc cttgctgaag ctgtgttctt ttctccagct
     8701 aatagtgtcg caatgccagc aaataccgat ttaccgcttc cgcctttccc tgttacttgg
     8761 aagaatactt gccagttgta gcggtttgtt aaaatcgcat agagagcggc tagaatgttc
     8821 ttcgccttgt cttgcttgcc gtctgataca aagttaagcc attgatcaaa gtgtggtgtt
     8881 tcggctacgt tctcgtcata gctatgtgga atataagccg ttagccagtt ttggcggtta
     8941 acggttcttt gaacatttac cgaataaggt gggtaagggg ttaaagtttc gggttgcccc
     9001 ttgctattcc aaccaatcac cgtagccggt tgtacttcca ttttttgaag cgtgccgaat
     9061 ggtcgggttt tatcgttggc tgtcgtgcgt ggataagcta agaagccttt agcttgtttt
     9121 gtgcctgtac cgttggttaa atcggtttct tcagtttcga caaaggattc tgaaatttct
     9181 tcagtcagcc agcctagaat atctacacca ccaaagtcta aaatctcttg cgtggttttc
     9241 gggtaagcgt aaatattatg aacggcaatc gttacttcat tcattttcgg ggtggcggtt
     9301 tcggtgcgtg cttcgccttc accgatatga tttaccgctg caccgccagc cgatactaat
     9361 tttttatatt ctttgccttg ggtcagtttc accacgttag caatttgtcg cataacagaa
     9421 tcatctgtta ggcgtttcat tacttgcttg tcaagttccg gaatcactga atagccaccg
     9481 tctgcccctc catttgtatt cgtagcaagc gaacgcaatt cgcctgtttt aatccagtgg
     9541 cgaagctcgt catcagatac tttcacattt gcggtttcaa cctgttgccc cggttggtta
     9601 cgctgttcat cagtaatcgc ttcataacgt gcgatttctt ttactaaatt ttcagcttgt
     9661 gagcggagtt catcaaactt agccccttct gcttcagttg ttgagcgttt ttcttcgtct
     9721 gctttggtta aaattgaacg catttctgct tcaatagccg ctttttgttg gcggagttct
     9781 aaaagttttt taaacattgc cttatcctta attcaaataa ttgatattgt agtgaagcgt
     9841 tgctgttttc cacgctgatt cgttagcatc ataatcatag ctatacttag ccagcacaca
     9901 ttcgtccata ctgtggaatg tggcttgatt catcacatca ctgatttttt gagccactaa
     9961 atctaggtcg ttttcggttg aaaacattgg taagtaaatg ccgattttca aataggcgtt
    10021 gcattcgcca tcgcaaagcg tgagttggct atattccgct tcatcaatga atacggcgat
    10081 agccggtaat tcattttcta catcggtgaa tacggctcgg ccgttatgga aatgttgaat
    10141 gccttgaatg ttaccttgta ataagtcgat aacttcttgg cggattgctt cgttagaaat
    10201 cataaaaaag tcctcttaat taagaaggct acaattagcc acagaacaat ataggcataa
    10261 tttcaacgga gtaaataggg taaaatttaa tcgtttagct acgtttgaac acgttaagag
    10321 gataattttt aaatgattat tttttattca acaaaagaca aaatggaagg aaataaacga
    10381 ataa
//
LOCUS       DANLZC010000091        10372 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.091, whole genome shotgun sequence.
ACCESSION   DANLZC010000091 DANLZC010000000
VERSION     DANLZC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.091"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..1247
                     /locus_tag="PXE44_001745"
     CDS             <1..1247
                     /locus_tag="PXE44_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDL5589238.1"
                     /translation="SAKDQIFFKNESFETGKSLNLDEVFDRNLSVETPNGFDVILEDK
                     KTGNLSGVNQSNIKALQFFIYQSQQTEEPLQRRFENSEIYGPFVVKSENHTYNQYFIK
                     AVDAQKEWINTILDSPFIMAVLLMFAGIPLLIWLSFKITKPVKELTISANAVASGNLE
                     TNPKLEAEWIYEIGEVGKSFNHMVISLKNLTQQQQRMISDISHELKTPLARLQLAIAI
                     LRRKTCGLAEIDRIEAEVGKLDQLIKDLLTISREQLNYQIHKKIFVIDEIWNNVIEDA
                     KFETSQNNIIFIIKQNIKNPESYSINGAVGLLASALENLIRNGQKYAKKMLSVSMNVE
                     DGHLFLTVEDDGEGVPENEYENILKPFYRVDEARARETGGAGLGLAIVQNVVKQHQGT
                     IHLDKSNMGGLKIELKLPLWRS"
     gene            1297..3219
                     /locus_tag="PXE44_001746"
     CDS             1297..3219
                     /locus_tag="PXE44_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HDL5589239.1"
                     /translation="MKLIEIENLNKYFGLGQTRAHILKNINLSIERGDFVAIIGASGS
                     GKSTLMNIIGCLDTASSGICRIDGKETQTMSADELSDLRQRKLGFIFQRYNLLSALNA
                     NENVALPAIYAGMDSSSRKKRADTLLTKLGLSDKTQHKPNELSGGQQQRVSIARALMN
                     GGEIILADEPTGALDSKSGETVLEILRDLHYEGHTIIMVTHDPNIAAQASRIVEIKDG
                     QIIRDERQRTDVQETALNKTTTQKPRFWNQLFESFKMATSAIMAHKMRAILTMLGIII
                     GITSVISVVALGRGSQQQILSNINNLGTNTMDIMNGTGFGDRRANLTKNLTISDALML
                     SRQNYVESTTPSSNVSATLIYGNTAVTGSVRGVGEQFIDVKGLKLISGRFFSAEEVKN
                     VAQVIVIDPNTQKDLGLTEPVEGKTILVDKKPLQIIGVAQQSNNMNQSSLTLWAPYTT
                     IMQRISGAKQIDSLTVKIKDDIESQTAEKSIAELLVAKHGKKDFFIMNSDTIKQTITS
                     TTNTMTLLISSIALISLIVGGIGVMNIMLVSVTERTREIGVRMAIGAKQRNILQQFLI
                     EAVVICLIGGVIGILLASAIIWAFNTLGTNFKMMLSPESVIIAVLCSTLIGVVFGYIP
                     ARNAAQLNPITALAQE"
     gene            3303..4073
                     /locus_tag="PXE44_001747"
     CDS             3303..4073
                     /locus_tag="PXE44_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL5589240.1"
                     /translation="MTITNTELEQILNTKLNSSAINDYAPNGLQVEGKREIKKIITGV
                     TATLPLIEKAIEKNADAILVHHGYFWKSENPCIRGMKGKRIKQLLVNDINLFGYHLPL
                     DIHSELGNNAQLAKHLGVKNLQGLEDRPNSIPMYGELENPISANELKQKLEKTLQRTV
                     ILCDEFVHSPQKMIKTIGICSGGGQGYIDLAFEKGCDAFISGEISEQTTHSAREQGIY
                     YFACGHHATERDGVKALGEWLAVEYGLDVEFIDIDNPA"
     gene            complement(4154..5284)
                     /gene="nrdB"
                     /locus_tag="PXE44_001748"
     CDS             complement(4154..5284)
                     /gene="nrdB"
                     /locus_tag="PXE44_001748"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL5589241.1"
                     /translation="MAYTTFSQHKNDQLKEPMFFGQNVNVARYDQQKYEIFEKLIEKQ
                     LSFFWRPEEVDVSQDRIDYAQLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWTFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISAYYDD
                     LIRDSQLYSLYGEGSYQVDGKTCEVSLRSLKRQLYLCMMSVNALEAIRFYVSFACSFA
                     FAERQLMEGNAKIIKFIARDEALHLTGTQHILNIMAYGNDDPEMAEIARECRQEAYEL
                     FLSAAEQEKAWAEYLFKDGSMIGLNRDILVQYIEYITNIRMQAVGLPLPFKARSNPIP
                     WINAWLVSDNVQVAPQEVEVSSYLVGQIDAKVDSRDFGDFEL"
     gene            complement(5311..7581)
                     /gene="nrdA"
                     /locus_tag="PXE44_001749"
     CDS             complement(5311..7581)
                     /gene="nrdA"
                     /locus_tag="PXE44_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL5589242.1"
                     /translation="MNKSLMVTKRNGTREQLNLDKIHRVITWAAEGLENVSVSQVELR
                     SHIQFYEGIRTSDIHETIIKAAADLITKDTPDYQYLAARLAIFHLRKKAYGQFEPPHL
                     YEHVKKLVALGKYDADLLSDYTPEEWETMNGFIDHWRDMTFSYAAVKQLEGKYLVQNR
                     VSGEIYESAQFLYLLVAACLFSKYPTASRLDYIRRFYDATSTFKISLPTPIMAGVRTP
                     TRQFSSCVLIECDDSLDSINATSAAIVKYVSQRAGIGINAGAIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATVYFPIWHLEAESLMVLKNNRGVEENRARHMD
                     YGVQINKMMYQRLIKGGEISLFSPSDVPGLYEAFFADQAKFEQLYLQYEQDPSIRQRK
                     VKAVELFSLLMQERASTGRIYIQNVDHCNTHSPFNPRVAPIRQSNLCLEIALPTKPLA
                     HFYDEQGEIALCTLSAFNLGTLSNLDELEELADLVVRALDALLDYQDYPVLAARNSSL
                     NRRALGVGVINYAYYLAKNQVRYSDGSANNLTHRTFEAIQYYLLKASMNLAKEQGACA
                     YFDQTTYAKGLLPIDTYKKEIDELTQEPLHYDWEQLRQDILQFGLRNSTLTALMPSET
                     SSQISNATNGIEPPRGYISVKASKDGILKQVVPEYERLKAYYELLWDIPNMDGYLHLV
                     GIMQKFVDQAISANTNYDPNRFAEAKVPMKVLLKDLLTAYKYGLKTLYYQNTRDGAED
                     VQDDLDEGCAGGACKI"
     gene            complement(7938..10286)
                     /locus_tag="PXE44_001750"
     CDS             complement(7938..10286)
                     /locus_tag="PXE44_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5589243.1"
                     /translation="MLISAQFSPLVWAENNDVAVLDEVSVVGSTPSISQGSEVTLLKV
                     SDKIIAGKEFKKRSATLGNALASELGVHSNPFGGGASKPIIRGQEGARIRILQNGSDV
                     IDMSNLSPDHAVVADSLLADQVEILRGSSTLLYASSSPVGIVNVVDKRIPTAIPEKGY
                     EVELNSRFDTAAKEKVGALGATFGIGNHIAVRVEGLSRHSDNYRVPGINLGERLNYVP
                     DTYNKSKVGTLGLSFVGERGYIGASYSKRRDNYGLPGHNHKFDFCTGHIYGNKRDKYA
                     YTYLYPHLIGEENIGSNPHFHCGTNHAEDGTHSHDNPFGHAHDHTHKGPWVDLESKRI
                     DVKAELRQPFKGVDKIKASYADADYYHDEKDAGVLATRYHKQLKKDQDYGKPVNIFKN
                     RGKNTRLEVYHAPLGGLTGVWGVQYQTQKSSMNAPKDREVKFPLVENTNKQFSLFGVE
                     QYMWDSVAVELAGRMEKQKIEIEYDRNEIKRLQEHYRISGGKQVEPDLSPYDETAYAY
                     SGTLNWFFHPDYQLSFTASHNERLPTPMELYYHGQHIATNSFEYGNKDLKKEQSNNVE
                     LGLGYQAERIGYKVSVYYNHFKNYIYNENLFRENQLFMRRYNQAKARFYGVEAEASYR
                     FNDQYQATIFGDMVRGWLTNLPPLKINSDYSVFKDYLPENVEKGKDYLLYRADQNTPR
                     TPPMRLGFRFNAEFTPNWSGDLELIRTFTQRRTSQLEYITEGNTMLNVGLSYSNKWKE
                     LDYKISLNGTNLLNQPVYIHTSYHQFVPQMGRNFMLGMEMKF"
BASE COUNT         3211 a         2159 c         1982 g         3020 t
ORIGIN      
        1 tttctgcaaa agaccaaatt ttctttaaaa atgaaagctt tgaaacaggg aaaagcttaa
       61 atttagatga agtttttgat agaaatttat cagttgaaac acctaatgga tttgatgtaa
      121 tcctagaaga taaaaaaact ggtaatttaa gtggcgtaaa ccaaagtaat attaaagcat
      181 tgcagttttt tatttatcaa tcacaacaga ctgaagagcc gttacaaaga cggtttgaaa
      241 atagtgaaat ctatggtcca tttgtcgtga aatccgaaaa ccatacttat aaccaatatt
      301 ttattaaagc ggtagatgct caaaaagagt ggattaatac gattttagac tcacctttta
      361 ttatggcagt attacttatg tttgccggaa ttcctttatt aatatggtta tcctttaaaa
      421 ttaccaaacc ggttaaagaa ttaactatct cggcaaatgc ggtagcctct ggtaatttgg
      481 aaactaatcc taaattagaa gcagaatgga tctatgaaat tggggaagtt ggaaaaagct
      541 ttaatcatat ggtgataagc ttaaaaaact taactcagca gcaacaaaga atgatttcgg
      601 atatatctca tgagttaaaa acacctttag ccagattaca actcgctatt gctatattac
      661 gccgtaaaac ctgtggtctg gcagaaatcg atcgcattga agcagaagta ggtaaattag
      721 accaattgat taaagacttg ttaacaatat cacgtgaaca attaaattat caaattcata
      781 aaaaaatttt tgtaattgat gaaatttgga ataatgtaat tgaagatgct aaatttgaaa
      841 cctcacaaaa taatattatt tttatcatca agcaaaacat caaaaacccc gaatcttatt
      901 ctattaacgg ggccgtaggt ttactggcta gtgctttaga aaatttgatc cgaaacggcc
      961 aaaaatatgc aaaaaaaatg ctgtcggtct caatgaatgt agaagatggt catttatttc
     1021 tcacagttga agatgatggc gagggggttc ctgaaaacga atatgaaaat attcttaaac
     1081 ctttttaccg cgtagatgaa gcaagggcaa gagaaacagg aggagccggg ctaggattag
     1141 cgattgtgca aaatgtagtt aaacagcacc aaggtactat tcatttagat aaaagtaata
     1201 tgggaggatt aaaaatcgaa ctgaaacttc ccctttggcg ttcctaaggc aaaaagcata
     1261 tcgtttgtaa aaaacagtcg aaaaataacc gcttgtatga aattaattga aatagaaaat
     1321 ctcaataaat actttggctt ggggcaaacc cgagcccata ttctaaaaaa tatcaatctc
     1381 agtattgaac ggggtgattt tgtcgcaatt ataggtgctt caggttctgg caaatctacc
     1441 ttaatgaaca ttattggctg tttagatact gccagctcag gtatttgccg tattgatggc
     1501 aaagagaccc aaacaatgtc tgcagatgaa ctgtctgatt tacgccaacg taaactgggg
     1561 tttattttcc aacgctataa tctcctttca gctttgaatg caaacgaaaa tgttgcccta
     1621 cctgcaattt acgccggaat ggatagctca tcacgcaaaa aacgagctga tacgttatta
     1681 acgaagttag gattatcaga taaaacccaa cataagccga atgagttatc tggggggcaa
     1741 cagcaacgag tcagcattgc acgagcctta atgaacggcg gtgaaatcat tcttgcagac
     1801 gagcctaccg gtgcattaga ttctaaaagt ggcgaaactg tattggaaat cttgcgagat
     1861 ctacactacg aaggtcatac cattattatg gtaacacacg acccaaacat tgccgctcaa
     1921 gccagccgta tcgttgaaat taaagatggg caaatcattc gagatgaacg ccaacgtact
     1981 gatgttcaag aaacggctct caataaaacg acaacacaaa aacctcgctt ttggaatcaa
     2041 ctgtttgaat ccttcaaaat ggcaaccagt gcaattatgg cgcataaaat gcgggcaatc
     2101 ctcacgatgc tgggcattat tattggcatt acctctgtga tttccgtagt cgctctcggt
     2161 cgaggctcgc aacaacaaat cctttctaat atcaacaacc taggcacaaa tacgatggat
     2221 attatgaatg gaaccggctt tggcgataga agagccaatc tgactaagaa tttaaccatc
     2281 agtgatgcct taatgttgag tcggcaaaac tatgtggaaa gcactactcc ttcaagcaat
     2341 gtcagtgcca cattgattta tggtaatacc gcagtaaccg gttcagtacg aggtgttgga
     2401 gagcaattta ttgatgtgaa aggattaaag ctgatttcag ggcgcttttt cagtgccgag
     2461 gaagtaaaaa atgtagcgca ggttattgta attgacccta acactcaaaa agatttgggg
     2521 ctaacagagc ctgttgaagg taaaacgatt ttagtggata aaaaaccgtt acaaatcatt
     2581 ggtgtggcgc aacagtcaaa caatatgaac caaagtagcc ttaccttatg ggcgccttat
     2641 accactatta tgcaacgcat ttcaggtgca aaacagatag attcactcac ggttaaaatt
     2701 aaagatgata ttgaaagcca aactgccgaa aaaagcattg ccgaattgtt agtagctaaa
     2761 cacggcaaaa aagatttctt tattatgaat agtgatacga tcaaacaaac cattaccagt
     2821 accaccaata caatgacttt gctgatctcc tctatcgccc ttatttcgct gattgtcggt
     2881 ggtatcgggg taatgaatat tatgctcgtt tccgtcaccg aacgcacccg agaaattggt
     2941 gtccgaatgg cgattggcgc aaaacaacgc aatattttgc aacaattttt aattgaggcg
     3001 gtggttattt gcctaatagg tggtgtgatt ggcattttac tggcaagcgc tattatttgg
     3061 gcatttaata ccttaggtac gaattttaaa atgatgcttt caccggaatc ggtgattatt
     3121 gccgtgctct gctctacact tatcggtgtg gtatttggct acattccggc aagaaacgcc
     3181 gcccaattaa acccaattac tgctctggca caagagtaaa tgcgataact atctgctaca
     3241 ctgtaggggc aaaaaatttt ttgcccctac atccattatt atgccaagtt taaggagata
     3301 aaatgacaat aaccaatact gaactagaac aaattctgaa caccaaactc aacagctccg
     3361 ccattaacga ctatgcccca aatggcttgc aagttgaagg gaaaagagag atcaaaaaaa
     3421 ttattaccgg tgtgacagct acacttcccc tgattgaaaa agcgattgag aaaaacgccg
     3481 atgcaatttt agtccaccac ggttattttt ggaaaagtga aaacccgtgc attcgaggaa
     3541 tgaaaggtaa acgcattaaa cagcttttag tgaatgacat caatctcttt ggttatcacc
     3601 taccactgga tattcactcc gaactaggta acaacgccca gcttgctaag catttaggag
     3661 taaaaaatct acaaggttta gaagacagac ctaattcaat tccaatgtac ggtgaattag
     3721 aaaatccgat ttccgccaat gaactgaaac aaaaacttga aaaaacgtta caacgcaccg
     3781 ttattttatg tgatgaattt gttcatagcc cacaaaaaat gattaaaaca atagggattt
     3841 gctccggcgg aggacaaggc tatattgatt tagcctttga aaaaggctgt gatgccttta
     3901 ttagtggcga aatttccgaa cagaccactc actctgcccg tgaacaagga atctattatt
     3961 ttgcttgcgg tcatcacgcc acggaacgag atggcgtaaa agccttaggt gaatggttag
     4021 ccgttgaata tggtttagat gttgaattta ttgatattga taaccctgca taaaaaatac
     4081 aagcggttga atttcctgaa aattttgcaa tttgcaaaaa atcactaaaa atcaaccgct
     4141 tgtatatcat agtctaaagt tcaaaatcgc caaaatcacg gctatctact ttagcatcaa
     4201 tctggccaac caaataagaa ctgacttcca cttcttgtgg tgctacctgt acattatcgg
     4261 aaaccaacca cgcattgatc cacggaatgg ggttggaacg tgccttaaat ggtaatggta
     4321 gtccaacagc ctgcatccga atattagtga tatattcaat atattgcacc aaaatatccc
     4381 gatttagccc tatcatcgaa ccgtctttaa acaggtattc cgcccacgct ttttcttgtt
     4441 cagctgcact taaaaaaagc tcgtaggcct cttggcgaca ttcacgtgca atttctgcca
     4501 tttcaggatc atcattgcca taggccataa tattcaatat atgctgagtg ccggtaagat
     4561 gtaatgcttc atcacgtgca ataaatttga tgattttggc atttccttcc ataagctgcc
     4621 gttccgcaaa ggcaaatgag caagcaaaag agacataaaa acgaattgct tcaagcgcat
     4681 tcacactcat catacagaga taaagttgtc tttttagact acgcaaactg acctcacagg
     4741 tttttccatc aacctgataa ctgccttcac catacaaact atagagttgg ctgtcacgaa
     4801 tcaaatcatc ataataggcg gaaatatctt tggcacgctt aataatttct tcgtttgtga
     4861 caatatcatc aaatactatt gatggatcat tgacaatatt acggatgata tgtgtgtaag
     4921 aacgactgtg aattgtttcg ctaaatgtcc aagtttcaat ccaggtttca agctcgggga
     4981 tagacactaa tggcaaaagc gccacattcg ggctacgccc ttgaatggaa tccaacaagg
     5041 tttggtattt taaattgctg ataaaaatgt gcttttcatg ttcaggcagt tgggcatagt
     5101 caatgcggtc ttgtgacaca tccacttctt ccggtcgcca gaaaaatgag agttgttttt
     5161 caatcagttt ttcaaaaatt tcgtattttt gttgatcata acgcgccaca ttcacatttt
     5221 gtccaaaaaa catgggttct tttagttgat catttttatg ttgtgaaaac gtagtatatg
     5281 ccatgttatt ctcctttatt tctgttctta ttaaatttta caagcaccac cagcacagcc
     5341 ttcatctaag tcgtcttgca catcttctgc tccatcacgg gtattttgat agtaaagggt
     5401 ttttaacccg tatttatacg ccgttaatag atcttttaat aatactttca tcggtacttt
     5461 agcttctgca aagcggtttg ggtcgtaatt tgtattcgcc gaaatcgctt ggtcaacaaa
     5521 tttctgcata atcccaacca aatgcaaata tccgtccata ttaggaatat cccaaagcag
     5581 ttcatagtat gcttttaaac gttcatactc cggcacaacc tgctttaaaa taccgtcttt
     5641 tgatgcttta acactaatat agccacgcgg aggttcaatt ccattggtgg cattcgaaat
     5701 ttgcgacgaa gtctccgatg gcattaaggc agttaaagtg gaattgcgca agccaaattg
     5761 cagaatatcc tgacgtagtt gttcccaatc ataatgaagc ggttcttgag ttaattcatc
     5821 aatttctttt ttataagtat cgatagggag taaccctttg gcataagtgg tttgatcaaa
     5881 ataagcacac gccccttgtt ctttggctaa attcatcgat gcttttaata aataatactg
     5941 aatcgcttca aaggtacggt gagttaaatt attagcactg ccatcggaat aacgcacttg
     6001 gtttttagct aaatagtaag catagttaat cacgcctaca cctaatgcgc gacgatttaa
     6061 tgagctgtta cgtgcagcta ataccggata atcttgataa tctaataggg catctagcgc
     6121 acgaaccact aaatcagcca attcttctaa ctcatctaaa ttgctgagtg tgcctaaatt
     6181 aaatgccgat aatgtacata atgcgatttc cccttgttca tcataaaaat gtgccagtgg
     6241 cttagttggc aaagcaattt ctaagcagag gttcgattgg cgtatcggtg cgacacgagg
     6301 attaaatggg ctatgagtat tacaatgatc gacattctga atataaattc gccctgttga
     6361 agcccgctct tgcatcagta atgaaaacaa ttctacagct ttaactttac gttgccgaat
     6421 gctcggatct tgctcatatt gcaaataaag ttgctcaaat ttagcttgat cagcaaaaaa
     6481 cgcttcatat aaccccggca catctgatgg actaaataaa ctaatctcac cgcctttaat
     6541 taaacgttga tacatcattt tattaatctg taccccgtaa tccatatggc gagcacggtt
     6601 ttcttccaca ccacggttat tttttaatac cattaagctt tctgcttcca aatgccaaat
     6661 cggaaaatac acggtagccg ctccaccgcg aacaccacct tgagagcaag actttactgc
     6721 agtttgaaaa tgtttataaa agggaataca gccggtatga aatgcttctc caccacgaat
     6781 ggggctacct aatgcccgaa ttgcgccggc attaataccg atcccagcac gctgtgaaac
     6841 atatttaacg attgcagccg aggttgcatt gatggaatct aagctatcat cacactcaat
     6901 taacacacag gaactaaatt gacgagtagg tgtacgcact cctgccataa tcggtgttgg
     6961 caaagaaatt ttgaaagtgg aagtcgcatc ataaaaacgg cgaatataat ctaagcggct
     7021 tgctgttgga tacttagaaa ataaacaggc agctacgagt aaatacaaaa attgagccga
     7081 ttcataaatc tctccgctaa cacgattctg cactaaatat tttccttcca actgctttac
     7141 tgctgcatag gaaaacgtca tatcccgcca gtgatcaata aatccattca ttgtctccca
     7201 ttcttcgggc gtatagtcgc tcagcaagtc tgcatcatat ttacctagcg caactaattt
     7261 tttaacgtgc tcatataaat gcggtggctc aaattgtcca taagcttttt tgcgtaagtg
     7321 gaaaatcgcc aagcgtgcgg ctaaatattg ataatctggc gtatccttag tgattagatc
     7381 agctgcagcc ttgataatag tttcatgaat atctgaagtg cgaatacctt cataaaattg
     7441 aatgtgagaa cggagttcta cttgtgaaac agaaacattc tctaaccctt ctgccgccca
     7501 agtaatcaca cgatgaattt tatctagatt gagttgctcg cgcgtgccat tacgctttgt
     7561 taccattaaa cttttattca taattattaa ccctttataa aataccttag taaaacacta
     7621 catcttgtgt ttttcattaa agtaaacaca agatagtgtg cttaaagcaa aacatcaacc
     7681 aataaatttt tactttttct cttgacagat gctaagttat ttataaataa ccatattttt
     7741 aataaaggga taaagttaat tgtcttattt aacaacaaac aggaaatttt ggtaagctcc
     7801 gaaaataaca agaaaggaaa aacagcacta ttttatccaa attaccaata cctgagtttt
     7861 aaacaagcgg ttatttttag ccgttttttt gcaaaatttg aagaaaaaat aaccgcttgt
     7921 cttgttatgt cgagaactta aaatttcatc tccattccca acataaaatt tcgtcccatt
     7981 tgtggtacaa attggtgata cgaagtgtgg atatacaccg gctgattcag taagtttgtg
     8041 ccgtttagac tgattttgta atccagctct ttccacttat tgctatacga caacccgaca
     8101 ttcaacatcg tattaccttc ggtaatatat tccaactggc tcgtacggcg ttgggtaaag
     8161 gtgcggatca gctctaaatc ccccgaccaa tttggggtaa attccgcatt aaagcgaaaa
     8221 cctaaacgca ttggcggtgt acgaggggtg ttttggtcag cccgatatag aagataatct
     8281 ttcccttttt ccacattttc cggcagataa tctttgaaca cactataatc gctatttatc
     8341 ttcaatggtg gtaaatttgt tagccagcct cgtaccatat cgccgaaaat cgtggcttgg
     8401 tattggtcgt taaagcggta gcttgcctct gcttctaccc cgtaaaaacg ggcttttgcc
     8461 tgattataac ggcgcataaa tagctgattt tcacggaaaa gattttcatt gtagatgtag
     8521 tttttaaagt gattgtaata aacactcact ttatagccaa tacgttcagc ttgatagccc
     8581 aagcccaact ccacattatt tgactgctct tttttcaagt ctttattgcc gtattcaaac
     8641 gaattagtag caatatgctg cccgtgataa tacagctcca ttggcgttgg caagcgttca
     8701 ttgtgcgagg cagtaaacga aagctgatag tcagggtgga agaaccagtt aagcgtgccg
     8761 gaataggcat aagccgtttc atcatagggt gaaagatctg gctcaacctg tttgccgcca
     8821 ctgatacggt agtgttcttg taaccgtttg atttcattgc gatcatactc aatctcaatc
     8881 ttctgctttt ccattcgtcc ggcaagctca acagcaacgc tatcccacat atactgctca
     8941 accccaaaca ggctgaactg tttattggta ttttcaacca gcgggaattt cacttcgcga
     9001 tctttcggcg cattcatact gcttttttgc gtttgatact gaacacccca tacaccggtt
     9061 aagccaccta gcggtgcgtg atagacctcc aagcgggtat ttttgccccg atttttgaaa
     9121 atattcaccg gcttgccgta atcctgatct tttttcagct gtttatggta acgggtggca
     9181 agcacgcctg catctttttc atcgtggtaa taatcggcat cagcatagct tgccttaatt
     9241 ttatctacgc ccttaaacgg ctggcgtagc tcggctttca catcaatccg ttttgattct
     9301 aaatccaccc acgggccttt gtgagtgtga tcgtgggcgt gtccgaacgg gttatcgtgg
     9361 ctgtgtgtgc cgtcctcggc gtggttggtt ccacagtgga agtgcggatt gctcccaata
     9421 ttttcctcac caatcaaatg cggatagaga taggtataag cgtatttatc ccgcttatta
     9481 ccgtagatat gaccggtaca gaaatcaaat ttatggttat gccccggcaa gccataatta
     9541 tcccgccgtt tgctgtatga ggcaccaata tagcctcgtt caccgacaaa ggataagccc
     9601 aacgtgccga ctttggattt attgtaagtg tccggcacat agtttaagcg ctcgcctaag
     9661 ttgatccccg gcacacggta gttatcacta tgacgactca gcccttcgac tcgcaccgca
     9721 atgtggttac cgatgccaaa ggttgcccct aatgcgccga ctttctcttt ggcggcggta
     9781 tcaaaacggc tattgagttc aacctcatat cccttttcag gaatagcggt cggaatacgt
     9841 ttatccacca catttacaat tcctaccggc gaactgctgg cataaagcaa ggtgctggaa
     9901 ccacgcaaga tttcgacctg atcggcaagc agtgaatcag caacaacggc gtgatcagga
     9961 gataggttcg acatatcaat cacgtctgaa ccgttttgca aaatgcgaat tctcgcccct
    10021 tcctgcccac gaataatcgg cttactcgct ccgccaccaa acgggttact atgcacgcct
    10081 aattctgacg ccaacgcatt acccagcgtt gccgaacgct ttttaaattc cttacctgca
    10141 ataattttat cactgacttt tagcaaggta acttcactcc cctgtgatat acttggagtg
    10201 ctgccaacga ccgaaacttc atctaacacc gctacatcgt tattttccgc ccaaacaagc
    10261 gggctaaatt gagccgaaat cagcaatgcc aatcctgttt tcttaaacac aatctttctc
    10321 cttaaaaaaa ttaaggataa taaggtactg caaaatatga gtctttacaa ta
//
LOCUS       DANLZC010000092        10272 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.092, whole genome shotgun sequence.
ACCESSION   DANLZC010000092 DANLZC010000000
VERSION     DANLZC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.092"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            184..561
                     /locus_tag="PXE44_001751"
     CDS             184..561
                     /locus_tag="PXE44_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDL5589244.1"
                     /translation="MEYQFTHSIHGVVAKCSMDHEAFARWLNAEITENPSQLAPIFAE
                     IEKCRAAFPNHYECVFEGREYSLYFDYDEVMAKANNLDAAFDDEEMEEGFQFYNEESI
                     AFCGLDDFEKFLKAYQHFVQTYH"
     gene            650..1237
                     /gene="yihI"
                     /locus_tag="PXE44_001752"
     CDS             650..1237
                     /gene="yihI"
                     /locus_tag="PXE44_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDL5589245.1"
                     /translation="MSRTKKTRRITDIMPARKTDKPKQPMPKLGGGKNRKLTRYELDA
                     QAREEKRKRKHKGLPTGSRNADPAEQKKAVVKEVKDPRIGSRKKVPLMVEFVNQPEKG
                     RTIKAVPVEPIKPTLSPEQELEQLENNECLNQLLDDLEAGKTLSAEDQKFMNECLDRI
                     DELMTELGIEYEDEEGDNGDALLRQFETMDINKFR"
     gene            1251..1718
                     /locus_tag="PXE44_001753"
     CDS             1251..1718
                     /locus_tag="PXE44_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2489 domain-containing protein"
                     /protein_id="HDL5589246.1"
                     /translation="MLRIFLMVLAALILISLGGYALHLMLKVRAQKKHEQALIEQAKQ
                     AQKDRYIRILDSIEVIARAMISEQCDFSEGVLRLKPLLDVLGRKLSDYTAMWALYGVV
                     ENMPILDERKNLKRNERMKLDLEREAKEAELESDIKNECYQLLKDIEEMRKAI"
     gene            1718..3085
                     /gene="hemN"
                     /locus_tag="PXE44_001754"
     CDS             1718..3085
                     /gene="hemN"
                     /locus_tag="PXE44_001754"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL5589247.1"
                     /translation="MSEIIWDLALIQKYNHSGPRYTSYPTALEFNENYTNEDFIRAAA
                     RYPERPLSLYVHIPFCHKLCYFCGCNKVITRHRHKVEIYLDYLEREIKTRAPLFQNRL
                     VTQIHWGGGTPTYLDEDQSARLMQMLRANFDVSDDAEISIEMDPREIELSMLDHLRAI
                     GFNRLSMGIQDFDKEVQRLVNREQDEEFIFALMKRAKELGFVSTNVDLIYGLPKQNVE
                     SFMYTLKRVVELNPDRMSVFNYAHLPSRFAAQVKIKEELLPAPETKLTILQNSIEFLG
                     KNGYKFIGMDHFAKPDDELAIAQEKGILHRNFQGYTTQEECDLLGMGVSAISLLGDTY
                     AQNRKELQQYYADVETNGIALHKGIALTQDDCIRRDVIKALICNFKLDFATIEKQYGI
                     DFKRYFAEDLAFLAPLAEDELLTMNDDSIVVSPRGRLLIRNICLCFDVYSRQLARRQQ
                     FSRII"
     gene            3159..5492
                     /gene="lptD"
                     /locus_tag="PXE44_001755"
     CDS             3159..5492
                     /gene="lptD"
                     /locus_tag="PXE44_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDL5589248.1"
                     /translation="MKQRYSLLSLAVMAACYSNSAAADLKQQCLLGVPHFQGEVVEGD
                     QLRMPVTIEADSAVINQPRDATYTGDVTIKQGNRSIWADQVRVEQDGENARMAYLQGN
                     FNYQDNLIQATGSDATINLLSKDANLANANYQLIGRQGRGTAESGEFGNSKHTLKNAS
                     FTSCLPNDNSWQIEANEMIQHVDEEYAEMWHARFKVLGMPVFYSPYLQFPIGDRRRSG
                     LLIPSFERSSKNGWTYSQPFYWNIAPNMDSTITPTYYSRRGWQISPEFRYLTKIGQGL
                     VATEYMAKDRLSEYQPGNDDRKRYLFHWRHAMSFLTDWRFSVDYTKVSDRRYFSDFDS
                     DYGSSTDGYATQNFKLGYYQPNYNISISGKKFQTFDELDIGPYRVLPQIDFNYYKNDL
                     IKGGDFKFFAQAARFENDSKLMPKAWRFHAEPTLNFPLANRYGSLNLETKLYATYYQQ
                     TKGSESTEEIKKSVSRVLPQIKLDFKTVLESDKQLFSGYTQTLEPRVQYIYRPYKDQS
                     DIGSKAHRSVSLGYDSALLQNDYFSLFNDRRYSGLDRISSANLVTAGGTTRFFKEATG
                     EEVFNFSLGQTYYISPSKIDDTSDNSTKGHTTSWAAESNWKFHKNWNWHGSYQYDTRL
                     NQTALANMSLQYKPQSDKLIQLNYRYASKDYINQNLNTNKYGQSIKQVGGVLGWELTD
                     KVSVMTSYYHDLALKKPVEGQISLNYNTCCWAANVYVARRLVSTPVGSSDTINDFYYD
                     NKFGINFELRFGTNYSNGVTRMLKRGIIPYTEPYSIN"
     gene            complement(5634..8273)
                     /gene="ppc"
                     /locus_tag="PXE44_001756"
     CDS             complement(5634..8273)
                     /gene="ppc"
                     /locus_tag="PXE44_001756"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDL5589249.1"
                     /translation="MNQQYSTIQHNIHMLGEFLGETIRDAQGNYILELIESIRVLSRN
                     SRAGDENAREQLLDKLANISNDDVLPVARAFSHFLNLTNIAEQHQTVSRHHIDESLGS
                     RSLDSLFLRLKSQNIPVEKVIKTVESLMIDLVLTAHPTEVTRRSLGHKHVEINKCLNL
                     LEHDDLTEAESYKIKRRLMQLIALAWHTNEIRTQRPTPVDEAKGGMAVIENSLWKAVP
                     EFCRQLNFYLEKHFGVQYPVNLAPVRFSSWMGGDRDGNPFVTAETTRRVLRMNRWKAT
                     ELFLQDIKNLAEELSITQCTPAFRAKYGNHLEPYRVLLKVLRQKLINTEIYYAELLEG
                     KISTIDESDILTSDQQLWEPLFDCYESLQACGMRIIANGALLDCLRRINCFGLGLSRL
                     DIRQESTRHEMAIAEITRYIGLGDYAHWTEDDKQAFLVRELSSRRPLIPTNWTPSPET
                     KEVLDTCKIVAEQPEGVISAYVISMAREASDVLSVHLLLKEAGCKFTIPVAPLFETLG
                     DLDHSEKVMTDLFNIGWYRGVINNYQMIMIGYSDSAKDAGMLAAGWAQYRAQEALVNL
                     CEKYNIELTLFHGRGGTIGRGGAPAHAALLSQPPRSLKNGLRVTEQGEMIRFKLGLPA
                     VAVESLELYASAILEANIFPPPEPKQQWRDVLAKASDISCQIYRDMVRGELDFVPYFR
                     SATPEQELSRLPLGSRPAKRNPKGGVESLRAIPWIFAWMQNRLMLPAWLGAGQALQQL
                     IDEGNETLLKEMCNEWPFFSTRIGMLEMVFSKADIWLAEYYDQRLVEPKLHRLGNMLR
                     TQLQADLKTVLALAHDGQLMADLPWIAESIALRNIYTDPLNLLQVELLDRIRKQGENA
                     DPVLEQALMITITGIAAGMRNTG"
     gene            8398..9366
                     /locus_tag="PXE44_001757"
     CDS             8398..9366
                     /locus_tag="PXE44_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family transporter"
                     /protein_id="HDL5589250.1"
                     /translation="MCLKLLFGFDNLPVVYVLFTVIQVGLSINGFKGIKWLENVSCVF
                     LIGILAYMLYVVNTKFSVEIGERFANIEGTWGMPFWAATTAFLGIYSTMILNASDYSR
                     HVKKGTTPTATGVIYVLSILPVTLFMGLIGLLVTAATGNSDPIEVFATAMDNKFLTVI
                     TLLFIAFAQVTTNVLNNIVPPSYILMDSFKMKWSHATILVGILSICVMPWKLVTAESA
                     DGLSLFIKIYSAFLGPIFAVMVVDYYILRKRTLNVNDLYNKEGIFKGINWAAIIAIAI
                     GSFCSLIVVDLSWYVSLIPSGLSYYILMRVLKSSEAFRKGTVLEHQ"
     gene            complement(9415..9585)
                     /locus_tag="PXE44_001758"
     CDS             complement(9415..9585)
                     /locus_tag="PXE44_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL5589251.1"
                     /translation="MLKCKQITELVSLSQEQKLTLKQRVNVQVHLMLCSRCRAFSQNC
                     KTVDKLMKEFNS"
     gene            complement(9586..10167)
                     /locus_tag="PXE44_001759"
                     /pseudo
     CDS             complement(9586..10167)
                     /locus_tag="PXE44_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         3089 a         1957 c         2187 g         3039 t
ORIGIN      
        1 tggcatagta gttccttttt gatgtatcaa tactaaaaat aacctgtatt attctagcag
       61 atttctgggt aatcgaggag ttgataacat ttaaaaatta gcgttttgca aaattttcca
      121 aaaaaagacc gcttgttagg ctataatagc caaccaaaca gaccaattca aaggagaatc
      181 ggtatggaat atcaatttac ccatagtatt cacggggtgg tggcaaaatg ttcgatggat
      241 cacgaggcgt ttgctcgttg gttgaatgcc gaaataacgg aaaatcctag ccagttagcc
      301 cctattttcg ctgaaataga gaagtgtcga gccgcatttc cgaatcatta tgaatgtgtg
      361 tttgaagggc gggagtatag tctctatttt gattatgatg aagtgatggc aaaagcaaac
      421 aatttagatg ctgcttttga tgatgaggaa atggaagaag gctttcaatt ttacaatgaa
      481 gagagcatcg cattttgtgg cttagatgac tttgagaaat ttttgaaagc ctaccaacat
      541 tttgtgcaaa cttatcattg attgaaatga ttaattgaaa ccaaagctat taagggaatc
      601 taattccttg tagaatagcc gtttatagca attttaagat attaaattta tgagtcgtac
      661 taaaaaaaca cgtcgtatta ccgacattat gccggcaaga aagaccgata aacctaaaca
      721 gcccatgcct aaattaggtg gggggaaaaa tcgcaaactg acccgttatg agttagatgc
      781 tcaagcccgt gaggaaaaac gtaagcgtaa acataaaggc ttgccgacag gctcacgcaa
      841 tgctgatccg gcagagcaga aaaaagcagt ggtgaaagag gtgaaagatc cgcgtatcgg
      901 tagccgtaaa aaagtgccgt taatggtgga atttgtgaac cagccggaaa aagggcggac
      961 gattaaggct gtgccggttg agccaatcaa accaacgctt tcaccggaac aagagctgga
     1021 gcagttagaa aataacgaat gtttaaatca gttgttagat gatttggagg cgggcaaaac
     1081 cttatccgca gaagatcaga aatttatgaa tgaatgttta gatcgcattg atgaattaat
     1141 gactgagctt ggcatcgaat atgaagatga agagggcgat aatggcgatg cgttattacg
     1201 ccaatttgaa acgatggata ttaataaatt ccgctaggta gccgattatg atgctaagaa
     1261 tattcctgat ggtgcttgcc gctttgattt taatttcgct gggcgggtat gcactgcact
     1321 taatgctgaa agtgcgagct cagaaaaagc acgaacaagc actgattgaa caggcaaagc
     1381 aagcacaaaa agatcgttat attcggattt tagacagcat tgaagttatt gctcgggcaa
     1441 tgatttcaga acagtgcgat ttttcggaag gcgttttacg tttaaaaccg ctattagatg
     1501 ttctcggcag aaaattaagc gactatacgg cgatgtgggc gttgtatggc gtggtagaaa
     1561 atatgccgat tttagatgaa cgcaaaaatt taaaacgtaa cgaaagaatg aagttggatt
     1621 tagagcggga agcaaaagaa gcggagcttg aatcagacat caaaaatgag tgttaccaat
     1681 tattaaaaga tattgaagaa atgagaaagg caatctaatg tcagaaatta tttgggattt
     1741 agcccttatt caaaaatata accattcagg gcctcgttat acctcttacc caacagcgtt
     1801 ggagtttaac gaaaattaca ccaatgaaga ttttattcgt gctgcagctc gttaccctga
     1861 gcgtccgctt tcgctttatg tgcatattcc gttttgccat aaactgtgct atttctgtgg
     1921 ctgtaataaa gtgattactc gtcatcgcca taaagttgaa atttatttag attatttaga
     1981 gcgtgaaatt aaaactcgcg cacctctgtt tcaaaatcgt ttagtgaccc aaatacactg
     2041 gggtggcggt acgccgactt atttagacga agatcaatcg gcacgtttaa tgcaaatgct
     2101 acgagccaat tttgatgtga gcgatgatgc ggaaatcagc attgaaatgg atccgcgtga
     2161 aatcgaatta agtatgttgg atcacctgcg tgctatcggt tttaaccgtc tgagtatggg
     2221 tattcaggat ttcgataaag aagtgcaacg tttggtgaat cgtgagcaag atgaagagtt
     2281 tatttttgcc ttgatgaaac gggcaaaaga acttggtttt gtttcaacta atgtggattt
     2341 gatttacggc ttgccaaaac agaatgtgga aagctttatg tacaccttaa aacgtgtggt
     2401 agagctaaac cctgatcgta tgagcgtatt taactatgcc cacttgccaa gccgttttgc
     2461 tgcacaagtg aaaattaaag aagagttgtt gccggctccg gaaaccaaat tgacgatttt
     2521 gcaaaattcc atcgaatttt taggcaaaaa cggttataag tttatcggta tggatcactt
     2581 tgctaaacct gatgatgagt tagctattgc acaagaaaaa ggcattttac accgtaactt
     2641 ccaaggctat accactcaag aagagtgtga tttattggga atgggcgtat cggcaatcag
     2701 cttactcggc gatacttacg cccaaaaccg caaagaatta caacaatact atgctgatgt
     2761 tgaaaccaac ggtattgcgt tgcataaagg cattgcgtta acccaagatg attgcattcg
     2821 ccgtgatgtg ataaaagcct tgatttgtaa tttcaaatta gattttgcca ccattgaaaa
     2881 gcaatatggt attgatttta aacgttattt tgccgaagat ttagctttct tagcgccgtt
     2941 ggcggaagat gaattattaa cgatgaatga cgacagcatt gtggtttccc cacgagggcg
     3001 tttattgatc cgtaatattt gcttatgctt tgatgtctat tcaagacaat tagcgagaag
     3061 acagcagttt tcccgtatta tttaatgagc aagcggtcga ttttggtaaa aattttgcaa
     3121 aaacagaccg cttgttcccg gcttataaag gaaaaatgat gaaacagcgt tatagcttac
     3181 tttctcttgc tgtgatggca gcttgttata gcaacagtgc cgcagctgat ttaaagcaac
     3241 aatgtttact tggcgtgccg cattttcagg gcgaagtggt agagggcgat caacttagaa
     3301 tgcctgttac cattgaggct gacagtgcgg tcattaacca gcctcgtgat gccacctaca
     3361 ccggtgatgt aacgatcaaa caaggtaacc gttcgatttg ggcggatcag gtgcgggttg
     3421 agcaagatgg cgaaaatgcc cgaatggcgt atttgcaggg taattttaac taccaagata
     3481 atttgattca ggcaaccggt tcagatgcga ccatcaatct gctttccaaa gatgcgaatt
     3541 tagctaatgc taattaccaa ttaattggca gacaagggcg tggcacggca gaatccggtg
     3601 aattcggcaa cagcaaacac acgctgaaaa atgccagttt cacttcgtgc ttaccgaatg
     3661 ataattcgtg gcaaattgaa gcgaatgaaa tgattcagca cgttgatgaa gaatatgcgg
     3721 aaatgtggca cgcccgtttt aaagtgctgg gaatgccggt attttattcg ccttacttac
     3781 aatttccgat tggcgaccgc cgccgtagtg ggttattaat tccaagtttt gagcgttcaa
     3841 gcaaaaacgg ctggacatat tcgcaaccgt tttattggaa tatcgcaccg aatatggact
     3901 cgaccatcac tccaacttac tattcacgcc gtggttggca aatcagcccg gaatttcgtt
     3961 atttaacgaa aatcgggcaa ggcttagtgg caaccgaata tatggcgaaa gatcgtttaa
     4021 gcgagtacca accgggaaat gatgatcgta aacgctattt attccactgg cgtcacgcaa
     4081 tgagtttttt aaccgattgg cgtttctcgg tcgattacac caaagtgagc gacagacgtt
     4141 atttctctga ttttgattcg gattatggta gcagtactga tggctatgcg acgcagaatt
     4201 ttaaattagg ctactatcag ccgaattata atatctcaat ttcaggtaaa aaattccaaa
     4261 ccttcgatga gttggatatc gggccttatc gcgttctgcc acaaattgat tttaactatt
     4321 ataaaaacga tctaattaaa ggcggagatt tcaaattctt tgcccaagca gcccgttttg
     4381 aaaatgatag taaattaatg ccaaaagcgt ggcgtttcca cgctgagcca acactgaatt
     4441 tcccattagc gaaccgctat ggtagcttaa atttagaaac caagctctat gcaacctact
     4501 atcaacaaac gaaaggtagt gagagtacgg aagaaattaa gaaaagtgta agtcgtgtac
     4561 tgccacagat taagctcgat tttaaaaccg tgttggaatc cgataaacag cttttctctg
     4621 gctatactca aacgcttgag cctcgggtgc aatatattta tcgcccatat aaggatcaaa
     4681 gcgatattgg ctcgaaagca catcgaagtg tgagcttagg ctatgactct gcattattgc
     4741 aaaacgacta tttctcactg tttaatgaca gacgttatag cggtttagat cgtatttcgt
     4801 cagcaaattt agtcaccgca ggtggcacaa cccgtttctt taaagaggca accggtgaag
     4861 aggtgtttaa tttcagctta ggacaaactt attatataag cccgtctaaa attgatgata
     4921 cctcagataa cagcactaaa ggtcatacga cttcatgggc agcggaatct aactggaaat
     4981 tccataagaa ttggaattgg catggtagct atcaatatga tactcgttta aaccaaacgg
     5041 cgttagcgaa tatgtcattg caatacaaac cacagtcaga caaattgatt cagcttaatt
     5101 accgctatgc aagtaaagat tacatcaatc aaaacttgaa cacgaataag tatgggcaat
     5161 ccattaaaca agtgggcggt gtgctaggtt gggaattaac cgataaagtg tcggtaatga
     5221 ccagttatta ccacgacctt gcattgaaaa aaccggtgga agggcaaatt tcgcttaact
     5281 acaacacttg ctgttgggcg gcaaatgtgt atgtggctcg tcgcttagtt tcaacgccgg
     5341 taggttcatc tgatacgatc aatgatttct actatgacaa taaattcggc attaactttg
     5401 agctacgctt tggcacaaac tacagcaatg gtgtaacccg tatgctaaaa cgaggtatta
     5461 ttccttacac cgagccgtat agcattaatt aacaaagtaa aaacaaataa gtaaaaatac
     5521 aaatcggggc gaaaaatatt tgccccgatt tttttatatt tggctgataa gcggttaaat
     5581 tctgttggaa ttttgcaaat cttccgctaa attcaaccgc ttgccatcga aacttaacct
     5641 gtatttcgca tacctgcggc aatacctgtg atggtgatca tcaatgcttg ctctagtaca
     5701 ggatcagcat tttcaccttg tttgcgaatc ctatctaaca attctacctg aagtagattg
     5761 agcggatcgg tataaatatt gcgtagtgca atcgattctg cgatccaagg taaatcggcc
     5821 attaattgtc catcgtgagc cagtgcaagt acggttttaa gatcagcctg tagttgcgta
     5881 cgcaacatat tgcctaaacg gtgtagtttt ggctcgacta ggcgttggtc gtaatattcc
     5941 gccagccaaa tatcagcttt actaaatacc atttccagca ttccgatacg ggtcgagaaa
     6001 aacggccatt cgttacacat ttcttttagt aaggtttcat taccttcatc aatcaactgt
     6061 tgcaatgctt gccctgcacc taaccaagca ggtagcatta agcggttttg catccaagcg
     6121 aaaatccaag gaattgcacg caaactctcc actccgcctt ttggattacg ttttgcaggg
     6181 cgtgagccaa gcggcagacg ggaaagttct tgctctggtg tagcagagcg gaaatacggt
     6241 acgaagtcta gctcgccacg caccatatca cggtaaattt ggcaagaaat atcggaggct
     6301 tttgctagca catctcgcca ttgctgcttt ggctccggtg gtgggaaaat attcgcttct
     6361 aaaatcgcac tggcatagag ttctaaactt tcaactgcca cggcaggtaa gcctaattta
     6421 aagcggatca tttctccttg ttcggtcact cgtaagccat ttttgagtga gcgaggtggc
     6481 tgagaaagta aggctgcgtg agccggtgcg ccaccacgcc cgatagtacc accacgaccg
     6541 tggaataagg ttaattcaat attatatttt tcacataaat taaccagagc ttcttgcgcc
     6601 cgatattgcg cccagccggc agcaagcatt ccggcatctt tggcggaatc agagtagccg
     6661 atcataatca tttggtaatt gttgatgacg ccacgatacc agccgatatt aaataaatcg
     6721 gtcatcactt tttcactatg atctaaatcg cctagcgttt caaataatgg ggcaaccgga
     6781 atagtaaatt tgcaccccgc ctctttgagt aaaaggtgaa ccgagagtac atcagaggcc
     6841 tctcttgcca ttgaaatgac ataggctgaa atgacacctt caggttgctc ggcaacaatt
     6901 ttacaagtgt ctaacacttc tttagtttca gggcttggag tccagttggt tggaatcaat
     6961 gggcggcgag aactgagttc acgcactaag aatgcctgtt tatcatcttc cgtccagtga
     7021 gcataatcgc ctaagccgat atagcgggta atttctgcaa tcgccatttc gtggcgggta
     7081 ctttcttggc gaatatctaa gcgtgataaa cctaaaccaa aacagtttat acgacgtaag
     7141 caatctagta atgcaccatt tgcaataatt cgcataccac aggcttgtaa tgactcatag
     7201 caatcaaaaa gcggttccca caattgctga tctgaagtta aaatgtcact ttcatcaata
     7261 gttgaaattt taccttccaa taattccgca tagtaaattt cggtattaat taatttctgg
     7321 cgtaatactt ttagtaatac gcgatatggc tctaaatggt tgccatattt agcacgaaat
     7381 gcaggggtac actgtgtaat cgataattct tcagcaagat ttttaatatc ttgtaagaac
     7441 aattccgtgg ctttccaacg gttcattctt aatacacggc gagttgtttc tgccgtgacg
     7501 aacgggttac cgtcacggtc tccgcccatc caagaagaaa aacggacagg ggctaaattt
     7561 accggatatt gcacaccaaa gtgtttttct aaatagaagt taagctgacg gcaaaattcc
     7621 ggcacggctt tccataaact attttcaatg actgccatac cgcctttggc ttcatcaaca
     7681 ggggttgggc gttgggttct gatttcatta gtatgccaag caagggcaat cagttgcatt
     7741 aaacggcgct tgattttata gctttctgcc tcagttaaat catcatgttc caacaaattc
     7801 aaacatttat tgatttcaac gtgtttatgc cccagcgaac ggcgggttac ttccgttggg
     7861 tgagctgtta gcactaaatc aatcattagg ctttccacgg ttttaatcac tttttcaacg
     7921 gggatatttt gtgattttag acgaaggaac agagagtcta atgagcgact accaagtgat
     7981 tcatcaatat gatggcgaga aacagtttga tgttgttcag caatatttgt tagatttaga
     8041 aaatggctaa atgcacgtgc aacgggtaag acatcatcgt tagaaatatt ggctaattta
     8101 tctaatagct gttcacgggc gttttcatcg ccggcacgag aattgcgaga taacacacga
     8161 atgctttcga tgagttctag aatgtaattt ccttgcgcat cacgaatcgt ttctcccaaa
     8221 aattcaccta acatatggat attatgctga attgttgaat attgctggtt cataaaaaat
     8281 tcctatggta aacataaatt aaattaggtc taaatatgcc gaaaatcgct gaaaatagca
     8341 aatagattta atttaatttt tgaattaaat gataaaacgc aaaaaatagc tgtcaatatg
     8401 tgtttaaaac tgttgttcgg ttttgataac ttgcctgttg tttatgtgct attcactgtt
     8461 attcaagttg gcttatccat taatggtttt aaaggtatta aatggttgga aaatgtttct
     8521 tgtgtgttcc tcatcggtat tcttgcttat atgctttatg ttgtgaatac aaaattctct
     8581 gtagaaatag gcgagcgctt tgccaatatt gaaggcactt ggggaatgcc attctgggct
     8641 gcaactacag cattcttggg tatttattca acaatgattt taaatgcttc cgattattct
     8701 cgccacgtta aaaaaggaac aaccccaaca gcaacaggtg tgatttatgt attatcaatc
     8761 ctccctgtaa ccttatttat gggtttaata gggttgttag ttacggcagc tacaggcaat
     8821 agtgacccaa ttgaagtatt tgcaactgca atggataata aatttttaac agtaataacg
     8881 ttattattca ttgcttttgc acaggttacg acaaacgtgt tgaataatat tgttccgcca
     8941 tcttacattt taatggattc atttaaaatg aaatggtcgc atgccaccat tttagtgggc
     9001 attctttcta tatgtgtaat gccttggaaa ttggtaactg ctgaatcagc agatggatta
     9061 tctttgttta ttaaaattta ctcggcattc ttaggcccta tttttgcggt aatggtcgtc
     9121 gattactaca tcttaagaaa acgtacttta aacgtaaatg acttatataa taaagaaggt
     9181 attttcaaag ggattaactg ggcagcaatt attgcgattg ctatcggttc gttttgctca
     9241 ctgattgtgg tggatctctc ttggtatgtc agtttaattc catcaggctt gagctactat
     9301 attttgatga gggtgttaaa aagttccgaa gctttccgta aagggacagt gctagaacat
     9361 caataagatg acaagcggta aaatttatta aaaattttac cgcttgcttt tacgctaaga
     9421 gttaaattct ttcattagct tatctacggt tttgcagttt tggctaaagg ctctgcaacg
     9481 agaacaaagc attaaatgaa cttgcacatt aacacgttgt tttaatgtca gtttttgttc
     9541 ttgggacaga gaaactaatt cggtaatttg cttacatttt aacatttaac ctccaaacca
     9601 tttttgactt aaacagacct gtaactgcaa tcttgcccga tacagtaagg tatgcacatt
     9661 tgctggtgaa atctcacatt ctgcgcaaat ttcttcggtt tccatttcca aatgggaacg
     9721 catcataaac acccttgctt gatttgccgg cagattattt aggcaaaatt caaatacttg
     9781 ccagaattga gcggaatagg tatggctttc ggtgttcttc cactcattag attgataagc
     9841 atctttatcc caaccgccat tttcgttaaa caatttatcg gcaagatcgt gatcgtcttc
     9901 ttccatcagc tcagatactg ccaccaattt gcctttggct ttcactaaat ctacgatctt
     9961 gttttttaaa attgcaaaaa tctacgtttt taacgcactt tcacctttga agctgtcggc
    10021 atatttataa gcgctgacaa acgcctcttg caccacatct tcggcaagtt cgttattttt
    10081 taactgtaaa aaggcaaact tcaccatttg cagacgaata tcttctagct gcgaggggga
    10141 aatttgccgt aaagccggtt ggctcattgc gtgatccttt ggtaaaaaat tgagagaaac
    10201 taaccgcttg tatcctattt cataaggttt tacccaactt aggggatatt ggtatgaagt
    10261 atttttaaac ct
//
LOCUS       DANLZC010000093        10039 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.093, whole genome shotgun sequence.
ACCESSION   DANLZC010000093 DANLZC010000000
VERSION     DANLZC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.093"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            18..542
                     /locus_tag="PXE44_001760"
                     /pseudo
     CDS             18..542
                     /locus_tag="PXE44_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            545..1009
                     /locus_tag="PXE44_001761"
                     /pseudo
     CDS             545..1009
                     /locus_tag="PXE44_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1277..2014)
                     /locus_tag="PXE44_001762"
     CDS             complement(1277..2014)
                     /locus_tag="PXE44_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5589252.1"
                     /translation="MIKIENLTLAHGIQHLSCYLPKGKLIGIMGANGAGKSSLLKAIA
                     GILRPTSGEIWLENCKLHALSAVERSRQFAYLAQQLNVAWQLSVYDVIALGLPAPLPP
                     EQERQKVRSVAEQLAISHLLDNTYQQLSGGEQPRVQLARCLMKQAPILLADEPIAALD
                     PYYQIDIMQQLKALTPTQTCVVVIHHLPLAYRFCDEIILLKNGNLLVSGNTQAVLNAE
                     NIATAFGVKAEMDWEKREMWGIEKLEV"
     gene            complement(2011..2979)
                     /locus_tag="PXE44_001763"
     CDS             complement(2011..2979)
                     /locus_tag="PXE44_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5589253.1"
                     /translation="MKKLNIVLGSLLLLIVSNAAFYQLSDFALLKNAEGVLSDLRWLV
                     LGEIRLPRIGLAVLTGASLALAGNAMQGVFKNPLASPGLLGSSSGATAVSVFLLYYFS
                     APMGWLLFGGVAGALGAFLLVWLISFRHGTTMMILSGVAVNVLLGAAVTLLLSNAESP
                     WALAELYRWLQGSLVWATAEAVCWALPLILLGIVCLYRERRYLDLLTFGEETAATMGI
                     NLQRSFFTVSIGVALLVGATIPQTGTIGFIGLIAPHLARIWLKKPPSQLYLTSALIGA
                     LLLLIADLAVQYVPFFARIYIGTLTAILGVPFLIWILFTQQWRLAQ"
     gene            3160..3495
                     /locus_tag="PXE44_001764"
     CDS             3160..3495
                     /locus_tag="PXE44_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5589254.1"
                     /translation="MYHIERTEIFDQWLENLKDPLAVVTIARRIERAESGNFGDTKYI
                     AEGISEMRLNVSKGYRLYYAQKGSIIYILLNGGHKGTQQADIDKAKMLWAEIKQHMAE
                     QEKKNERII"
     gene            3479..3802
                     /locus_tag="PXE44_001765"
     CDS             3479..3802
                     /locus_tag="PXE44_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5589255.1"
                     /translation="MNELYDAPIPQATREKFGIQPFDAAEYLANDELIALYLAETLKD
                     GTDEEFIQALNTVARAKGMNELAKKAGIGRESLYQSLSSSKPRFETIRKIISALNLEL
                     SVVKA"
     gene            complement(3792..5678)
                     /locus_tag="PXE44_001766"
     CDS             complement(3792..5678)
                     /locus_tag="PXE44_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5589256.1"
                     /translation="MTQLDSVTVYSAYATPINQDKTASSVTVLTEKDFAARNATYVSD
                     VLKTVPGVAIGQQGGRGTLTSLFLRGAESRHTAVVIDGVKVNPINIGNFDFGGLPISN
                     IERIEVLRGEQSALWGSSAMGGVVYITTKSGLYKEKPFNAEVDLGLGSNNTRDASATL
                     SGFHNGFYYALHGDSHRTKGISALSKNHFSYTTETGSEVKTGGASERDGFHRDNGSLR
                     LGYDLGNKGVEVLAAQSSQTVHIDGYNSDVSGEYSRTRNQTFKLGGYWGNEQELLKHQ
                     ANISQFNSKATHFGSNARYSNEKQLNANYQLDVNFDREGEVTQAVSLLTDYAKTRYTS
                     DKYLREKTLSEKSAALEYRLFTEQDHSFSISGRYTDNSQFKNSITGRISGAYRLSPNL
                     CSDRLLLELAEPQQIRAMSPYSQKPLMMLDKLNTDKPTVEPELTALLPYADSTILLNE
                     TFYPQLTARLKQLGFKLVALNDSPQTPEQLFTLILQLGELTQNQAKAEKLVERLRLQK
                     IPLKQPLAETLILSETGMIEPHFPQYQTLLDLLGLSPLKSDLTPQNFSLEKLLLAQPK
                     QLLFLTDNQSYNNQAELLKHPALQKIWQKMSQNPPLVLPMKYTYCFDHGVWQGIQLMH
                     KLTP"
     gene            5708..6508
                     /gene="metF"
                     /locus_tag="PXE44_001767"
     CDS             5708..6508
                     /gene="metF"
                     /locus_tag="PXE44_001767"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDL5589257.1"
                     /translation="MQQKWRMLWDSIDRLSPLKPKFVSVTYGANSGERDRTHSIVKSI
                     QEKTGIISAPHLTGIDATPEELKVIAKDYWDSGIRRIVALRGDIPKGYDKALFYASDL
                     VALLKSVADFDISVAAYPEVHPEAKSAQADLLNLKRKIDAGANHVITQFFFDVESYLR
                     FRDRCVSIGIDCEIVPGILPVTNFKQLQKMAAVTNVKIPTWMGKMYEGLDDDQTTRNL
                     VAASIAMDMVKVLSREGVNDFHFYTLNRSELTYAMCHTLGVRPDLTTA"
     gene            complement(6557..7717)
                     /locus_tag="PXE44_001768"
     CDS             complement(6557..7717)
                     /locus_tag="PXE44_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589258.1"
                     /translation="MKKSILLILSLFSASLFADVQLSPLPLPQIGKVSQFDLSEQGEL
                     VVINQQKQLWRVTQQAQKLADGVSTEIEPKAQYQRIALADQNGHFMLWTADKIYTSNI
                     PLAPKATMTALNFATIAVTKQNGQFKLARIETQGNQATITATAETEVLPDAHPLQIDF
                     DAETPNQGHIAVLAKPDDFTYQHGVLGDDVEAGEIQFLERHTLNPLAEKLTQQGRVFE
                     ANRWATFTENGKTKIVSVISGDGNGASTVVIGVKDGKLSIEAESEPLSTNRWQSPFIF
                     NRKLYAIQMPHLKGNLVEYSLKGNQLAERFITSGLSNHRYGDHETNLVATTETFALIP
                     QMGYQQVVVLDKTGKITELPTALPAGIKQTKADSNKAYLLLKNGEIWVAEEK"
     gene            complement(7777..8793)
                     /gene="galE"
                     /locus_tag="PXE44_001769"
     CDS             complement(7777..8793)
                     /gene="galE"
                     /locus_tag="PXE44_001769"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL5589259.1"
                     /translation="MAILVTGGAGYIGSHTLVELLNENREIVVLDNLSNSSEVSLERV
                     KQITGKSVKFYQGDILDRDILRKIFAENQIESVIHFAGLKAVGESVREPLRYYQNNVT
                     GSIVLVEEMLKANVNTIVFSSSATVYGDPQIIPIVESCPVGGTTNPYGTSKYMVERIL
                     EDTVKAFPQLSAVVLRYFNPVGAHESGLIGEDPNGIPNNLMPFISQVAVGKLPQLSVF
                     GGDYNTHDGTGVRDYIHVVDLALGHLKALDKHQNDAGFHVYNLGTGTGYSVLDMVKAF
                     EAANGITIPYKVVDRRPGDIAVCYSAPQKALEQLGWETERGLEQMMKDTWNWQKNNPN
                     GYKG"
     gene            complement(8881..9042)
                     /locus_tag="PXE44_001770"
     CDS             complement(8881..9042)
                     /locus_tag="PXE44_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HDL5589260.1"
                     /translation="MNETLLNNLVCPVSNEKLEWDKENNRLINPKLNIAYPIKNGIPE
                     LLPEAGVAL"
     gene            complement(9020..10021)
                     /gene="lpxK"
                     /locus_tag="PXE44_001771"
     CDS             complement(9020..10021)
                     /gene="lpxK"
                     /locus_tag="PXE44_001771"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDL5589261.1"
                     /translation="MKIWQTKSLITWLLSPLSLLFWLISQARKRLYSLNILQSYRSPL
                     PMLVVGNISVGGNGKTPVVVWLVEQLQQRGVKVGVISRGYGGKAKTFPQLVTRESSAE
                     MVGDEPVLIVQRTNVPLAISPNRQQSIELLLNQFEPDIIITDDGLQHYALQRDMEWVV
                     VDGVRRFGNGFVLPAGGLRELPSRLNSVQAVICNGGTPQANEHLMTLEPSDAINLVTN
                     QRKPLSEFTQPCVAIAGIGHPPRFFTMLEKLGIPLAKTQGFADHQAFDKAQLAQFADS
                     QTPLLMTEKDAVKCHDFAQPNWWYVPVSATFSEKSTACLLAPILQKLGKEDERNTTQ"
BASE COUNT         2793 a         2161 c         2216 g         2869 t
ORIGIN      
        1 ctaagcctta tggtttattg gcgagatctt cttattttgc acgtttagca gaatggactt
       61 tattagaaca tttacttgtc aaacagcctg aaaatatttc ggtatgttca ttcggtcaat
      121 cattcggtac aagcggtcgt ttagatgcag aatattacca acctaaattt gcaagcttat
      181 ttgcacaatt agccaatttc aaaacgatga aattaggtga gattgttaat ttacaaaaat
      241 cggtagaacc gggctcggaa gcttatcaaa cagagggaat accctttgtg cgtgtatcgg
      301 atttatctaa attcggcatc agccaaacag ataaatactt acatccgaaa gattttggca
      361 atgttgtaag acctaaaaaa gacagtatat tactgactaa agacggcaca gtcggcattg
      421 cttaccgtgt tccgcaagat ttgaatgtga ttacatcagg ggcaattgtg catctagagc
      481 ttaaaactga cgaagtttta cccgattatt tggcgttagc attgaattct cctgctgtac
      541 aattcaaaac aaactttata aagagtttga aagaatattc acctctggaa aaataaaaga
      601 aaaaactaat ttatggaata atattgagca acttcatttt gcattagttc aggcagagtt
      661 aaaatattca ggtagcaata ttccaagtga atattctaaa tttaacttgg aaaatgtgct
      721 tattgatttt cctaacaaga caaattctgt agcatatcag aatctagtta ttcacaacgc
      781 aaaaacgcgt gcaggaaaat taaaaacaca atatgcaaat gctcacgatt taggcaattt
      841 ggttttagta gaggatatcc aaaaaatatt ttccgggtta ttagaaataa tttatcaagt
      901 tcgttgccat ttggtacatg gtagcttaag tccaacacct gaaaaccacg aagttgttaa
      961 atattgctat ttgattttgt gggattgttt aaaaggattt tgtgattagt tattgtcaat
     1021 aaagctacaa gcggtcaatt ctcccaaaac tttgcaaatt tccccgcaaa actgaccgct
     1081 tgttctcctc tatccattca cctcaaccaa cttttctctc tacccctact caaaatcggt
     1141 taaacaatct gttgaggtaa atgttgtgtc ggaatgggat ttaatcttaa tgaaactgaa
     1201 aaaatcttct gccaagcaaa tttccctaaa attggaagtt catttccaat ttctcaaaaa
     1261 atcacccaaa ccggaactac acttccaatt tttcaatccc ccacatttcc cttttctccc
     1321 aatccatttc agctttcacc ccaaaagccg ttgcaatatt ttcggcattt aagaccgctt
     1381 gtgtgttgcc gctgacgagt aaattgccgt ttttaagcag aatgatttca tcacaaaaac
     1441 ggtaggcaag gggcaaatga tgaatcacca ccacgcaggt ttgtgttgga gttagggctt
     1501 tgagctgctg cataatatcg atttggtagt aggggtcgag ggcggcaatc ggttcatcgg
     1561 caagcaagat cggggcttgt ttcatcaaac aacgggcgag ttgcactcgg ggctgttcac
     1621 cgccggagag ctgttggtaa gtgttgtcca gcaggtgaga aatcgccaat tgttcggcaa
     1681 ccgaacgcac cttttgacgc tcttgttcag gcggaagcgg tgcgggcaag ccgagtgcaa
     1741 tcacatcata caccgaaagt tgccacgcca cgttgagctg ttgggcgagg taggcaaatt
     1801 gtcggctcct ctcaacggcg gaaagggcgt gcagtttgca attttccagc caaatttcac
     1861 cgcttgtcgg gcgtagaatg ccggcaatcg ctttgagcaa cgaggatttc cctgccccgt
     1921 ttgcgcccat aatgccgatc aatttgcctt ttggcagata gcaagagagg tgttgaatac
     1981 cgtgggcaag ggtgagattt tcaatcttga tcattgtgcc aatctccatt gctgggtgaa
     2041 taaaatccaa atcaaaaacg gtacgcccaa aattgccgtg agcgtgccga tgtaaattcg
     2101 ggcaaaaaac ggcacatatt gcaccgccag atctgcaatc agcagtaaca acgccccgat
     2161 taaagcggag gtaagataaa gctgggacgg cggttttttc agccaaatac gggcaagatg
     2221 aggggctatc aagccgataa aaccgatggt gccggtctgt ggaatcgttg ctccgaccaa
     2281 cagcgccacg ccaatgctaa cggtaaaaaa actgcgttgc aggttgatgc ccattgttgc
     2341 tgccgtctct tcgccaaagg tgagcaggtc gagataacgc cgttcccgat agaggcagac
     2401 aatgccgagc aagatcaacg gtaacgccca acatactgcc tctgcggttg cccagactaa
     2461 tgagccttgc aaccagcggt agagttccgc caacgcccac gggctttcgg cattggaaag
     2521 cagtaaagtc accgctgccc cgagcaacac gtttaccgcc acgccactta aaatcatcat
     2581 tgttgtgccg tggcgaaagc taatcagcca caccagcaaa aatgccccca gcgcccctgc
     2641 cacaccgcca aacagtagcc agcccatcgg ggctgaaaaa tagtagagca gaaatacgct
     2701 caccgccgtt gcccccgaac tgctcccaag caaaccgggg ctggcaagcg gatttttaaa
     2761 cacgccctgc atcgcattgc cggcaagcgc aaggctggcg cccgtcagca ccgccaagcc
     2821 aatacggggc agtcgaattt cacccagcac cagccagcgc agatcgctta gcacgccttc
     2881 cgcattttta agcaaggcaa aatcgctcag ttggtaaaag gctgcgttgc taacaatcag
     2941 caacaacaag cttccaagca caatgtttaa tttcttcatt cttctttctc tcatcaaacg
     3001 tgcaaattat accatagcgc caaagtcaag ctgaaaacgc tatttttgcg atcatcatcg
     3061 aaaaactagc gtttgccgaa tgtaacccac gacaggcttt gctatattcc ttcaatcagc
     3121 ctaagttttg tatttaaaac attacagatt gggggtagaa tgtaccatat tgaacgcact
     3181 gaaattttcg accaatggct tgaaaattta aaagatccac ttgctgtggt gaccattgca
     3241 agacgtattg aacgtgccga aagcggaaat tttggcgata ccaaatatat cgcagaagga
     3301 atttccgaaa tgaggctaaa cgtcagcaaa ggttatcgcc tttactatgc gcaaaaaggt
     3361 agcattattt atatactact aaatggcgga cataaaggca cgcaacaagc tgatattgat
     3421 aaagctaaaa tgctatgggc agaaattaaa caacatatgg cagaacagga gaaaaagaat
     3481 gaacgaatta tatgacgccc caattccaca agctactcgt gaaaaatttg gcattcaacc
     3541 tttcgatgcc gctgaatacc tcgccaatga cgagctaatc gccctttatt tggcggaaac
     3601 attgaaagac ggcacggacg aggagtttat tcaagcattg aataccgtgg cacgagcgaa
     3661 aggaatgaat gagttagcga aaaaagcagg gatcgggcgt gaaagtttgt atcaaagcct
     3721 ttccagcagt aagccccgtt ttgagactat ccgcaaaatt attagcgcat taaatttgga
     3781 gttgtcggtc gttaaggcgt aagtttgtgc attaattgta tcccctgcca aaccccgtgg
     3841 tcgaaacagt aggtgtattt catcggtagg acaagcggtg gattttggct cattttttgc
     3901 catatttttt gtaatgctgg gtgtttgagc agttcggctt ggttgttata gctttggtta
     3961 tcggtgagga acaataactg tttgggttgc gctaacagca gtttttctag cgagaaattc
     4021 tgtggcgtga ggtcgctttt taatggtgac agaccgagaa gatccaacaa cgtttgatat
     4081 tgcggaaaat gcggttcgat catacccgtt tcgcttagaa ttaaagtttc agcaagcggt
     4141 tgttttagcg gaattttttg caaacgtagc cgttccacca gcttttccgc tttggcttga
     4201 ttttgggtta gctcgcccag ttgtaaaatc agtgtgaaaa gctgttcggg tgtttgcgga
     4261 ctgtcgttta atgcgactaa cttaaagcca agctgtttca gccgtgcggt gagttgcgga
     4321 tagaaggttt cattcagcaa aatggtgctg tcggcatagg gcaacagggc ggtgagttca
     4381 ggctcaacgg tgggcttgtc cgtgttcagt ttatccaaca tcatcaacgg cttttgggaa
     4441 tagggcgaca tcgcccgaat ttgctgtggt tcggcaagtt ccagcaacag acggtcgcta
     4501 cacaaatttg gcgataaacg gtaagctcct gaaatacgcc cagtgataga atttttaaac
     4561 tgtgaattat cggtgtaacg gccactaata gaaaaactgt gatcttgttc ggtaaataag
     4621 cgatattcta gtgccgcact tttttcggat aaggtttttt cacgcaaata tttatcagag
     4681 gtatagcgtg tttttgcata atctgtcagt aaactgaccg cttgtgtcac ttctccttca
     4741 cggtcaaagt ttacatctaa ttgataattc gcattaagtt gtttttcgtt gctgtagcga
     4801 gcatttgaac cgaaatgtgt tgctttacta ttgaattggc tgatatttgc ttggtgtttc
     4861 agcagctctt gttcatttcc ccaataaccg cctaatttaa aagtttgatt gcgagtacgg
     4921 gaatattcac cgctaacgtc tgaattatag ccatcaatat gaacggtttg ggaagattga
     4981 gcggcaagaa cttctacccc tttgttaccg aggtcataac ctaaacgtaa agagccgtta
     5041 tcacgatgaa agccatctcg ttcgcttgca ccaccggttt tcacttctga gcctgtttct
     5101 gtggtatagc tgaaatggtt tttactcagt gcggaaatgc ctttggtacg atggctatca
     5161 ccgtgtaagg catagtagaa accgttgtga aatccggaaa gtgttgcgct ggcatcacgg
     5221 gtattatttg agcctaaacc aagatcgacc tccgcattga atggtttctc tttgtataaa
     5281 ccgcttttag tggtgatata aactacacca cccatagcac tgcttcccca aagggcggat
     5341 tgctcgccac gcaacacttc gatacgttcg atattgctaa tcggtaagcc accaaaatcg
     5401 aaatttccaa tattaatcgg attgactttt acaccgtcaa tcacaaccgc agtatggcga
     5461 gattctgccc ctcgtaaaaa taagctagta agagtaccac gcccaccttg ttgcccaatc
     5521 gccacgcccg gcacagtctt taacacatca ctcacataag tcgcattgcg tgcggcaaaa
     5581 tctttttcgg taagaaccgt cacgctggaa gcggttttgt cttggttgat tggggttgca
     5641 taggcggaat agactgttac ggaatcaagc tgagtcatct cattttgtgc aaaagctgta
     5701 cttgctagtg caacagaaat ggcgaatgct gtgggattct attgatcgcc taagtccttt
     5761 aaaaccgaaa tttgtttcgg taacttatgg agcaaattct ggtgagcgag atcgtacaca
     5821 cagtattgtg aaatcaatcc aagaaaaaac agggatcatt tcagctccgc acttaacagg
     5881 cattgatgca acaccggaag aattaaaggt aattgccaaa gattattggg atagtggtat
     5941 tcgccgaatt gttgctttgc gtggcgatat accgaaaggt tatgataaag cactatttta
     6001 cgcctcagat ttagtcgctt tattaaaatc agtagcagat ttcgatattt ccgtggctgc
     6061 ctaccctgaa gttcaccctg aagcaaaatc agcacaggca gatttactta acttgaaacg
     6121 taaaatcgat gcgggtgcaa accacgttat tacgcaattc ttttttgatg tagagagtta
     6181 tttacgtttt cgtgatcgct gtgtttctat tgggattgat tgtgaaattg tgccgggtat
     6241 tctgccggtg acaaatttca agcagttgca aaaaatggca gcagtgacta atgttaagat
     6301 cccgacttgg atgggtaaaa tgtacgaagg gttagatgat gatcaaacca ccagaaactt
     6361 agttgcagca agtattgcaa tggatatggt gaaagtgctt tctcgtgaag gggtgaatga
     6421 tttccacttc tataccttaa atcgtagtga actcacttat gcaatgtgcc atactttagg
     6481 cgtaagacct gatttaacaa ctgcataatc gaacattaag gctgtatagc taaatgagct
     6541 atacctcctt atagttttac ttctcttcag ccacccaaat ctcgccattt tttaataata
     6601 aataggcttt gttggaatcg gctttggttt gtttaattcc agccggcagg gcggttggca
     6661 attcagtgat cttgcctgtt ttatccaaca caacaacttg ttgatagccc atttggggta
     6721 tcagtgcgaa agtctctgtt gtcgccacta aattggtttc gtgatcacca tagcggtgat
     6781 tgcttaagcc ggaggtaata aaacgttcag ctaattggtt tcctttcagg ctgtattcaa
     6841 ccaaattgcc ttttagatgt ggcatttgga ttgcatagag cttgcggtta aagataaacg
     6901 gcgattgcca acggttagtc gacagcggct cactttcggc ttctattgat aattttccgt
     6961 ctttaacgcc aatcacaaca gtgctggccc cattgccatc gccagagata acagaaacga
     7021 tcttggtttt tccattttct gtgaaagtcg cccagcggtt ggcttcaaat actcgccctt
     7081 gttgggtgag tttttctgct aaggggttta gggtatggcg ttctagaaat tgaatttcgc
     7141 ctgcctctac atcatcgcct aatacaccgt gttgatatgt aaaatcatca ggctttgcaa
     7201 gtacagcaat atgcccttgg ttaggtgttt cggcatcaaa atcaatttgc agagggtgag
     7261 catcaggcaa cacctcggtt tctgcggtgg cagtaatagt ggcttggttt ccttgcgttt
     7321 caattctggc taacttaaat tgtccgtttt gtttggtcac ggcaatggtg gcaaaattaa
     7381 gggcggtcat tgtagctttc ggggcaaggg gaatgttgga ggtatagatt ttatcagccg
     7441 tccacagcat aaaatgcccg ttttgatccg ctaacgcaat tcgttggtat tgagctttag
     7501 gttcaatctc ggtggaaact ccatctgcca gtttttgagc ttgttgtgtg actctccata
     7561 attgtttttg ttggttaatt accactaatt cgccttgttc tgaaaggtca aattggctta
     7621 ctttgccaat ttggggaagt gggagtggag aaagctgaac atcagcaaac aaagaagcgg
     7681 agaacaagct taagattaac agaatagatt ttttcattag cgttacctaa aaagaagatt
     7741 taaatgataa agggagatgt tatctccctt tacgctttag cctttataac cattcgggtt
     7801 atttttttgc caattccaag tgtctttcat catttgttct agcccacgct cggtttccca
     7861 acccagttgc tctaacgctt tttgtggagc ggagtagcaa acggcaatat cgcccgggcg
     7921 gcgatctacc actttgtatg gaatagtaat gccatttgcg gcttcaaagg cttttaccat
     7981 atctagcacc gaataacctg ttcctgtgcc taaattgtaa acgtggaaac cggcatcatt
     8041 ttggtgcttg tctaaggctt ttaagtgacc aagtgctaaa tctaccacgt ggatataatc
     8101 acgcacacct gtgccatcgt gagtattata atcgccacca aacaccgata attgaggtaa
     8161 tttgcccaca gccacttggc tgataaacgg cattaagtta tttgggatac cgtttggatc
     8221 ttcaccgatt aaaccgcttt cgtgagcccc taccgggttg aagtaacgca atactaccgc
     8281 acttaattgt gggaaggctt taacggtatc ttcaagaatg cgttcaacca tatatttgga
     8341 cgtaccgtaa gggttggttg tgccaccgac agggcaagat tccacaatcg ggataatctg
     8401 cggatcacca taaacggtcg ccgatgaact aaacacaatg gtattcacat tggcttttag
     8461 catctcttct accaatacaa tcgagccggt tacattgttt tgatagtaac gtagcggttc
     8521 tctgacgctt tcgcctacag cttttaaacc ggcaaagtga attaccgatt caatctgatt
     8581 ttccgcaaaa attttgcgta aaatatcacg atctaaaata tcgccttgat agaattttac
     8641 gctcttgccg gtgatctgct tcacacgctc aagtgatact tcagaggaat tagaaagatt
     8701 atctaacacc acgatttcac gattttcgtt taataattca actaatgtat gtgaaccgat
     8761 gtagcctgca ccacctgtaa ctaaaattgc cattttatgt tcctctataa aattaatacg
     8821 aaccaaggtt attgttgcta ttttacccga tttaatcagg cttgctaata tttattcagt
     8881 ttataatgcc actccggctt ccggcagtaa ttccggaatg ccgtttttaa tcgggtaagc
     8941 aatattgagc ttagggttaa ttaatcggtt gttctctttg tcccattcta atttttcatt
     9001 agagacaggg cagacgaggt tattgagtag tgtttcgttc atcttctttt cctagttttt
     9061 gtagaatcgg ggctaacaaa caagcggtcg atttttctga aaatgttgca gaaaccggta
     9121 cataccacca atttggctgt gcaaaatcgt ggcattttac cgcatctttc tcggtcatta
     9181 ataacggcgt ttggctatca gcaaattgag ccagctgagc cttatcaaag gcttgatgat
     9241 cggcaaagcc ttgagttttg gcaagcggaa tgcctaattt ttctaacatc gtaaaaaagc
     9301 gtggtgggtg accgataccg gcaatcgcca cacagggttg ggtaaattcg cttaatggct
     9361 tgcgttgatt ggtgactaaa ttaattgcat cagacggttc aagcgtcatt aaatgctcgt
     9421 tagcttgcgg tgtgccgccg ttgcaaataa ccgcttgcac ggagtttaaa cggctcggta
     9481 attcacgcaa tccgcccgcc ggcagcacaa aaccgttacc gaaacgcctt acgccatcaa
     9541 ccaccaccca ttccatatct cgctgtaggg catagtgttg caagccatca tcggtgatga
     9601 tgatgtccgg ctcaaactga tttaatagta attcaatgct ttgttggcgg tttggagaaa
     9661 tagccaaagg cacattggtt cgctgaacaa tcagaaccgg ttcatcaccc accatttcag
     9721 cagaactttc tcgggtaact aattgcggaa acgttttcgc tttaccgcca tagcctcgag
     9781 aaatcacgcc tacttttacg ccacgctgtt gcagttgttc caccagccaa acgaccactg
     9841 gcgttttgcc gttgcctcca accgagatat taccgactac cagcataggc aaaggcgaac
     9901 ggtacgattg caaaatattc agagaatata accgcttgcg agcttgacta atcagccaaa
     9961 acagcaacga aagcggcgaa agcagccaag tgataaggga ttttgtttgc cagattttca
    10021 tattatttat ttttaagtg
//
LOCUS       DANLZC010000094         9967 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.094, whole genome shotgun sequence.
ACCESSION   DANLZC010000094 DANLZC010000000
VERSION     DANLZC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.094"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(417..1457)
                     /locus_tag="PXE44_001772"
     CDS             complement(417..1457)
                     /locus_tag="PXE44_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL5589262.1"
                     /translation="MKKQLRKNLTQNDRLQPQAQTEIFSFGDPIPVLDRADILNYLEC
                     SAMYEKWYNPPMSFDGLAKSLRSSTHHESAIITKANILLSTCEVDSRYLSRRDLSSFV
                     KDYLVFGNAYFEVVRNRLGQVQRIESPLAKYVRKGLEAGQFYYVPQRFDHQEHEFAKG
                     SIYHLLEPDINQDIYGLPQYLSALQSAWLNESATLFRRKYFLNGAHAGFVFYMSDASQ
                     KQEDVENIRQQLKQSKGVGNFKNLFVHAPNGKKDGIQIIPIADVSAKDEFFNIKNVSR
                     DDVLAAHRVPPQLMGIIPNNTGGFGNVADAAEVFFITEIEPLQERLKEFNQWLGQEVI
                     KFKPSKLLQRTQ"
     gene            complement(1466..3268)
                     /locus_tag="PXE44_001773"
     CDS             complement(1466..3268)
                     /locus_tag="PXE44_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDL5589263.1"
                     /translation="MENENTITEYEVLAANPLHDKREAQSKYWAGYTVTEISRQLNIP
                     VSTIASWKKREKWDEISPVGRVEATLESRLNLLIMKESKNNNDYKEMDALRRLLESTA
                     RIKKYSNGGGNEADLNPNIKNRNKGDRKKPEQNAISEEQAELLINGFLDGMFHYQKKW
                     HEAGLTHRIRNILKSRQIGATYYFAHEALVDALVTGRNQIFISASKKQALQFRAYIVA
                     YAKRVADVELKGETITLPNESQLIFLGTNSKTAQSYHGNLYFDEIFWVNRFEEIRKVA
                     AGMASQKQYRITYFSTPSSITHSAYLLWSGKLFNRKRPKAEQVEIDISHANLKNGKKC
                     GDGQWRQIVNIYDAEAGGCNLFDIEQLKLENSPDEFEQLFMCEFIDDNQSVFKFTMMQ
                     RCLVDSMEVWRDYVFTDGYQRPFGNKEVWVGYDPSYTGDRSALVVIAPPKVDGGKFRL
                     LEYRTFKGADFAEQAAEIVAICAKYNVTRLAIDTTGLGVGVYEIVKKERPDAVALTYN
                     VELKSKMVLKGLDIISKGRFEFDSMHAVEVGASFMAIKKQITNSGRQVTYVADRSEEA
                     SHADLAWACLQVFINEPFDGKLEETTGTVDFLDD"
     gene            3418..4245
                     /locus_tag="PXE44_001774"
     CDS             3418..4245
                     /locus_tag="PXE44_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDL5589264.1"
                     /translation="MTTKSKWFVVATEGATTDGREISREWLTQIEENYDPKNNYGARI
                     NLEHFRWRWFEKDDPHSYSYGDVLAVKTEEREDGKLQLLAEIAPTEALVKLVKDKQKV
                     YTSVEIDLNFADTGEAYLVGLAVTDTPASLGTEYLTFCAGATHNPLADRKQKPENLVS
                     EAVEANLEFTAEPEKGAWLEKFKAMFTKAKTDNDGKFAEHEKAMELLAEQFSKVSTEN
                     DRLQTELSTLQIEFTELQKQAGEFAEKFAKLEQEPSANYTPRPKATGAKAGDVQTDF"
     gene            4259..5287
                     /locus_tag="PXE44_001775"
     CDS             4259..5287
                     /locus_tag="PXE44_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDL5589265.1"
                     /translation="MKDLTLEKYNAYLARQAELNNLPFNALATGIKFTVQPSVQQKLY
                     EKVRESSDFLKSISFVFVDEQTGETLGLDSAHTVASTTDTSGDGERKTTSIAKLVKQT
                     YHCQQINFDTHINYKQLDMWAKFPDFQQKVANVAAKQRKRDLIMIGFNGTSRAATSDR
                     NSNPLLQDVAKGWLQKMREDAKERVMNGESTDNQVLVGKGQEYANLDALVMDATEELI
                     DEWHRDDTDLVVITGRKLLADKYFPIVNQQNAPTEELAADIVISQKRIGGLKAVRVPF
                     FPANAILITKLENLAIYVQEGTTRKHIENVPKKDRIETYESENIDYVVEDYGCAALIE
                     NITLKDKE"
     gene            5297..5986
                     /locus_tag="PXE44_001776"
     CDS             5297..5986
                     /locus_tag="PXE44_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HDL5589266.1"
                     /translation="MRMSPARAHFLRKTAESESATPNRENLEGLQGYDLVLAQLKAHQ
                     RQLKKIQSIERKIEFKRNNFEQYRPWVEGSLAKGSGVQDTVITTMLIWSIDIGDYPTA
                     LAIAQYVLMHDLAMPEQFERTPATALVEELAEAAKKARDQKQPFEAAVLKQANDLTAE
                     FDMPDPVRAKLLRELGELIQEAEPQTALEHYQRAIALDPQCGAKGLRDKLEKQLAKQA
                     EEPKPAEEQNE"
     gene            6098..6613
                     /locus_tag="PXE44_001777"
     CDS             6098..6613
                     /locus_tag="PXE44_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDL5589267.1"
                     /translation="MEQRNHVISIPKVNGYESSKNAIKPTAESDETITNNGFFPNIEL
                     LEVRNAMRIDGTVINERLKQAVIEAMATVNADLKAYRLNAEQAQKANLQACDDEQING
                     ESVLVYKYKRAVYCLAVANLYERYRSYDSTKDGHDKAEELESTAGDLKRDYHFAVRDI
                     LGENRMISELI"
     gene            6610..6822
                     /locus_tag="PXE44_001778"
     CDS             6610..6822
                     /locus_tag="PXE44_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL5589268.1"
                     /translation="MNTLIARQDDTLDELIFRHYGQTAGLVEQALEYNPELAHLPRLP
                     IGTVVTMPDIESRFATVAKSSVQLWD"
     gene            6828..7034
                     /locus_tag="PXE44_001779"
     CDS             6828..7034
                     /locus_tag="PXE44_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HDL5589269.1"
                     /translation="MHKTEQASYFGAIFGFFGSLTLSDWGVVVSILVAILTLLLNWAY
                     RAKEYKLKEREFELKLQNQGKKNE"
     gene            7027..7593
                     /locus_tag="PXE44_001780"
     CDS             7027..7593
                     /locus_tag="PXE44_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL5589270.1"
                     /translation="MSKSVKVGVFVCSVAAIIGTVKQYYSSEIRTSETGLAIIGNAEG
                     CRRDPYKCPADVITVGIGSTEASGEKINVNHKYTDKEIAERWIKDLKIAERCINRYFN
                     GDKMNENQFSAMVSAAFNMGCYNLRFYPNENGKYIQTTIHKYAMAKDFKAMCNRIPDF
                     NRSGGKVLRGLKIRREKEKALCLGQEVK"
     gene            7590..8045
                     /locus_tag="PXE44_001781"
     CDS             7590..8045
                     /locus_tag="PXE44_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589271.1"
                     /translation="MIVKLCQMTNKHFSPLMIVLVLVGVLANFVQFRQNESLKADLQA
                     RTTQNQLIQADNRNLANQLENSQIQLEAYQKQVDDLNQKVLAKMKQAEQRSNEILNEL
                     EKHKKWADSAVPPSVGKLLNQRKRAVSHPQIEPDNLSEKAGVPNTGNRD"
     gene            8194..8415
                     /locus_tag="PXE44_001782"
     CDS             8194..8415
                     /locus_tag="PXE44_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDL5589272.1"
                     /translation="MSDQIDRANELAEKAREAALAKILQNQTACTSLFECEDCGEPIP
                     EKRREMVIGCTRCIECQTIYEHKQKGYRR"
     gene            8412..8897
                     /locus_tag="PXE44_001783"
     CDS             8412..8897
                     /locus_tag="PXE44_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5589273.1"
                     /translation="MIKPDRLRAVLTQTIDIFQANPEKLILQYDKGKIKSKGSQSHSF
                     EYHYDLELIVVDFPYHPDVLFVPVLNFVRNEQWELLQNPELQDKIEFEIDHNNHESYD
                     IYIRLPLTERVIVKEQDGHLTATHADEPNLADVSPFTRLTEYEVYLKDELIYQWAEPN
                     E"
     gene            8890..9348
                     /locus_tag="PXE44_001784"
     CDS             8890..9348
                     /locus_tag="PXE44_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL5589274.1"
                     /translation="MSEPIEQVKTAFDSLLNNISKPRRRLMYQQIGRELDRSQRRRIK
                     AQQNPDGSAYEPRKKPKKGVKSKIKSGKMFDKITQPRFMRLRLESEGVSLGYEGGDAV
                     IARIHQQGLIGRVRKDWDLKVKYASRELLGFTDDDLQMIEDYMINILAGS"
     gene            complement(9399..9677)
                     /locus_tag="PXE44_001785"
     CDS             complement(9399..9677)
                     /locus_tag="PXE44_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589275.1"
                     /translation="MEILFILLGLIGFGALLAIGFTTFAAIFGVVTTVFLSLLSLLAN
                     MVGLSIIVILISLYVLLPLNTFILIALIFSAILGFLCWYHSDRQSVNQ"
     gene            9716..9967
                     /locus_tag="PXE44_001786"
                     /pseudo
     CDS             9716..9967
                     /locus_tag="PXE44_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248190.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3118 a         2178 c         2174 g         2497 t
ORIGIN      
        1 tcattattta tcaataagtt aaaatctata atgtgcagta tatgtatatg caattaaaga
       61 ataacgattt ttcctgacaa gcaatatttt tgcgttgagg tgcgtaaaat tgcgtaaaaa
      121 gatcgagttt ttaagatcgc tttgcgatta tatggggctt gtagggatcg cctttttgca
      181 tttgcgtata aatcgacaaa ttaagtgtgc gggcgaggtg agggatagac cgcgcggggt
      241 tggaggtggt gaagtcgttg aaaaataaag gaaaaattat tttttaagtg tattttattt
      301 gttgttttga tataataaat tgccgaaata tataatctaa tcaggcaaaa cttttgcttt
      361 atatattaaa aaagccccaa tctaggagct gtattgtcag tttgtaaaga tctgttttat
      421 tgtgtccgct gtaacaattt agacggttta aatttgataa cctcttgacc taaccactga
      481 ttaaactctt tcaatcgctc ttgcaatggc tcaatctcgg tgataaaaaa cacctcggca
      541 gcatcagcca cgttgccaaa accgccggtg ttatttggga taatccccat cagttgtggt
      601 ggcacacggt gagcagctaa cacatcatca cggctcacgt tcttgatatt aaaaaactca
      661 tcttttgccg acacatcagc aatcggaata atctgtattc catctttctt gccgtttggt
      721 gcgtggacga acaagttttt aaaattcccc accccttttg actgcttgag ctgctggcga
      781 atattttcaa catcttcttg tttttgtgac gcatcgctca tataaaacac aaagccggcg
      841 tgagctccat ttaaaaaata cttacggcga aacagagtag cactctcatt taaccacgca
      901 gattgcaggg ccgaaagata ttgtggcaag ccgtaaatat cttgattaat atcaggctca
      961 agcaagtgat agattgagcc tttggcaaat tcgtgttctt ggtgatcgaa tcgttgtggc
     1021 acatagtaaa attgaccagc ctctaaaccc tttcgcacat attttgccaa aggtgattcg
     1081 attcgctgta cctgcccaag gcgatttcgc accacctcaa aataggcgtt gccaaatact
     1141 aaataatctt taacaaacga tgacaaatca cggcgtgata aataacggct atcaacctcg
     1201 caagtggaaa gcaaaatatt tgctttggtg atgatcgcac tttcgtggtg tgttgatgac
     1261 cttaacgatt tcgccaagcc atcaaagctc atcggtgggt tataccattt ctcatacatc
     1321 gccgaacact ccaaataatt gagaatatcg gcacggtcta acaccggaat cgggtcacca
     1381 aaactgaaaa tttcggtttg tgcttgcggt tgtaagcggt cattttgggt taaatttttg
     1441 cgcaattgtt ttttcatttt ttgtcctaat catctaaaaa atctactgtg ccggtcgttt
     1501 cttccagttt cccatcaaac ggctcattaa taaatacctg caagcaagcc cacgcaaggt
     1561 cggcgtggct tgcctcttcc gaacggtctg ccacataagt cacttgccgt ccgctgttgg
     1621 tgatctgttt tttaattgcc ataaaactcg caccaacctc aacagcgtgc attgagtcaa
     1681 attcaaatcg tcctttgctg ataatatcca agccttttag taccatcttg gatttcagct
     1741 ctacgttgta agtaagagcc accgcatctg gtcgttcttt tttgacgatt tcataaacac
     1801 cgacaccaag ccctgtggta tcaatcgcca gtctggtcac gttatatttt gcacaaatag
     1861 cgacaatttc tgccgcttgt tcggcaaaat cagccccttt aaaagtgcga tactctaata
     1921 gtcggaattt cccgccgtcc actttcggcg gagcgattac caccaacgcc gagcggtcgc
     1981 cggtgtaaga cgggtcatac ccaacccata cctctttatt gccgaatggg cgttggtagc
     2041 cgtccgtgaa tacataatct cgccacacct ccatcgagtc cacaaggcaa cgttgcatca
     2101 tcgtgaactt gaacacagac tggttatcat caataaactc acacataaag agctgctcga
     2161 actcatcggg cgagttttcc agtttgagtt gctcaatatc aaacaggtta cagccacctg
     2221 cctcggcatc gtagatattc acgatttgtc gccattggcc gtctccgcat tttttgccgt
     2281 ttttcaagtt ggcgtgagaa atatcaattt ccacctgttc cgctttcgga cgtttgcggt
     2341 taaacagctt gcccgaccaa agcagatagg cggaatgggt aatgctcgaa ggggtagaaa
     2401 aataagtaat ccgatattgt ttttggctcg ccatacccgc tgccactttg cggatttcct
     2461 caaagcggtt tacccaaaag atttcatcaa aatagagatt gccgtggtaa gactgtgccg
     2521 ttttcgagtt tgtacccaag aaaattagtt gagactcgtt cggcaaggta atggtttcgc
     2581 cttttagctc cacatcggca acccgtttgg cgtatgccac aatataggct ctaaattgca
     2641 atgcctgttt tttcgaggcg gaaataaaaa tctgatttct accggttact aacgcatcaa
     2701 ctaacgcctc gtgagcaaaa taataagtcg ctccaatttg acgactttta agaatattgc
     2761 ggattcgatg agtcagccca gcttcgtgcc attttttctg ataatggaac attccgtcta
     2821 aaaagccgtt tatcaacagt tcggcttgct cttcggaaat ggcattttgc tcgggctttt
     2881 tgcggtcgcc tttattacgg tttttaatat ttgggttgag gtcggcttca ttacctccgc
     2941 cgttagaata ttttttgatt cgtgctgtac tttccaacaa ccgacgtaaa gcatccattt
     3001 ctttgtagtc gttattgttt ttgctctctt tcataatcag caaattcagc cgtgattcca
     3061 gtgtggcttc cacacgtcca acggggctga tttcgtccca tttctctcgc tttttccaac
     3121 ttgcaatggt agagacggga atatttagct gacgagaaat ttcggtcacg gtgtaacctg
     3181 cccaatactt tgattgagcc tctcgtttat cgtgtagagg gttggcagcc aacacctcgt
     3241 attcagtgat tgtattttca ttttccatag gtgcgattat caaaaatcca ccttgtttaa
     3301 gcatttgctt gaggctgtga aacccgattt aacacgtacc acacattgaa accgccggca
     3361 caaaacaaga tcataaatcc gatttttacc cctccccttt agagcaagga aaaacagatg
     3421 acaacaaaat ccaaatggtt tgtggtagca acggaaggag caactacaga tggacgcgaa
     3481 attagtcgtg aatggctcac gcagattgaa gaaaattacg acccgaaaaa taactatggt
     3541 gctcgtatta accttgagca tttccgctgg cgttggtttg agaaagatga cccgcattct
     3601 tacagctatg gcgatgtgtt agcggttaaa acagaagaac gtgaagacgg caaattacag
     3661 ttactggcag aaatcgcccc aaccgaagcc ttagtgaagt tagtcaaaga caagcagaaa
     3721 gtttatacct ctgtggaaat tgaccttaat tttgcggaca caggcgaggc gtatttagtt
     3781 ggtttggcgg tgactgatac accggcaagc ctcggcacgg aatacctcac attttgtgcc
     3841 ggtgcaaccc ataacccact agcagaccgc aagcaaaagc cggaaaacct tgtgagtgag
     3901 gcagtagagg cgaatttaga atttactgct gaacctgaaa aaggtgcgtg gctggagaaa
     3961 tttaaggcga tgtttaccaa agccaaaacc gacaatgacg gcaaatttgc cgaacacgaa
     4021 aaggcaatgg aactgttagc tgagcaattt agcaaagttt cgacagaaaa cgaccgcttg
     4081 caaactgaac taagcacatt gcaaatcgaa tttaccgaat tgcaaaaaca agcgggcgaa
     4141 tttgccgaaa aatttgcaaa actggaacaa gaaccgtctg ccaattacac cccacgccca
     4201 aaagccactg gtgcgaaagc aggtgatgta caaaccgatt tttaattgag gatcacgaat
     4261 gaaagattta acccttgaaa aatataacgc ctaccttgca cgccaagctg aattaaacaa
     4321 cttgccgttt aatgcattgg caaccggcat taaattcaca gtgcaaccat cggtacagca
     4381 aaagctgtat gaaaaggtgc gtgaaagctc tgatttctta aaatctatca gctttgtgtt
     4441 tgttgatgag caaaccggtg aaaccttagg tttagattca gctcacaccg tggccagcac
     4501 caccgatacc agcggtgacg gtgaacgcaa aaccacctct attgcgaagt tggtaaaaca
     4561 aacctaccac tgccaacaaa tcaattttga cacccatatc aattataaac agttggatat
     4621 gtgggcgaaa ttccctgatt ttcagcaaaa agtggcgaat gttgcggcaa aacaacgcaa
     4681 acgtgactta attatgatcg gctttaacgg cacaagtcgt gcggcaacca gtgatcgcaa
     4741 cagcaacccg ttattgcaag atgtggcaaa aggctggttg cagaaaatgc gtgaagatgc
     4801 caaagagcgt gtaatgaatg gcgaaagcac agacaaccaa gttttagtcg gcaaaggtca
     4861 agagtatgcc aatcttgacg cactcgtaat ggacgccacc gaagaactta ttgatgaatg
     4921 gcatcgtgat gacaccgatt tagtggtgat taccggtcgc aagttgcttg cggataaata
     4981 tttcccgatt gtgaatcagc aaaacgcacc aaccgaagaa cttgccgctg atattgtgat
     5041 ctcacaaaaa cgcattgggg gcttaaaagc ggtgcgtgtg ccgttcttcc cggcgaatgc
     5101 gattttaatc actaagctgg aaaatttagc catctatgtg caagagggga caacccgcaa
     5161 gcatattgag aacgtgccga aaaaagaccg tattgaaacc tacgaatcag aaaatatcga
     5221 ctacgttgtc gaagattatg gctgtgcggc attaatcgaa aacattacac tcaaagataa
     5281 agagtaaggt ggaactatgc gaatgagtcc ggctcgggct cattttttgc ggaaaaccgc
     5341 agaaagtgag tctgcaaccc caaaccgaga aaaccttgaa ggcttacaag gctatgactt
     5401 agttttagcc caacttaaag cccatcaacg ccaattaaag aaaatccagt cgattgagcg
     5461 aaagattgag tttaaacgca acaattttga gcaataccga ccttgggtgg aaggctccct
     5521 tgccaaaggc tccggtgtgc aagacacggt tatcaccaca atgctgattt ggtcgattga
     5581 tattggcgat tacccaaccg cccttgcgat tgctcaatac gttttaatgc acgatttggc
     5641 aatgccggag cagtttgaac gcacaccggc aaccgccttg gttgaagagc ttgcagaggc
     5701 ggcaaagaaa gcccgagacc aaaaacagcc gtttgaggca gccgtgctga aacaggcaaa
     5761 cgacctcacc gctgaatttg atatgcccga cccagtgcga gccaaattac tgcgtgagct
     5821 aggcgagcta atccaagagg cagagccaca aaccgccctt gaacactatc aaagagcgat
     5881 agccctcgac cctcaatgcg gtgcaaaagg cttgcgagac aagttagaaa agcaacttgc
     5941 caaacaagcc gaagagccga aaccagcaga agaacagaat gagtaggcga aagccgaaat
     6001 agagcgtatc cacgccagcc aaggggcgga ttttaaaggg tgatttattc attttgcacc
     6061 cttttaaaat ccccacccct taccttacgg aaaccttatg gaacaacgca atcacgttat
     6121 tagtatcccg aaagtgaatg gctatgaatc cagcaaaaat gcgatcaagc ccaccgcaga
     6181 atctgatgaa actatcacca ataatggctt ttttccgaac attgagctac tggaggtgcg
     6241 aaatgcaatg cggattgatg gcacggtcat taatgagcgg ctaaaacaag cggtgattga
     6301 ggcaatggca acagtcaatg ccgacttaaa agcctatcgc ctcaatgcag agcaagcaca
     6361 aaaagcgaac ttgcaagcct gtgatgatga gcaaatcaac ggcgaaagcg tgctggttta
     6421 caaatataaa cgagcggtct attgccttgc agtagcgaac ctttacgaac gctaccgcag
     6481 ttatgacagc accaaagacg gccacgacaa agccgaagag ctagaaagta ccgccggtga
     6541 tttgaaacga gattaccact ttgccgtgcg tgatattttg ggcgaaaacc gaatgattag
     6601 tgaattgata tgaacacctt gattgcacgc caagacgaca ccttagacga gctgatcttt
     6661 cggcactacg ggcaaacggc aggcttagtc gagcaagcct tagaatataa ccccgaactg
     6721 gctcatttgc cacgtttgcc gatcggcact gtagttacaa tgccggatat tgagagccgt
     6781 tttgcaacgg tggcgaaatc cagcgtgcaa ctttgggatt aaaaaatatg cacaaaactg
     6841 aacaagcctc ctattttggg gcaatttttg gcttttttgg tagcctcacc ctatctgatt
     6901 ggggggtggt agtgagtatt ctggtagcta ttttaacctt attacttaat tgggcatacc
     6961 gagccaaaga atacaaactg aaagagcgag aatttgagct aaagctacaa aaccaaggga
     7021 aaaagaatga gtaaaagtgt aaaagttggc gtatttgtct gctccgttgc agctattatc
     7081 gggacggtaa agcaatatta cagctccgaa atccgcacca gcgaaacagg cttagccatt
     7141 atcggcaatg cggagggttg cagacgtgat ccgtataaat gccctgccga tgtaatcacg
     7201 gttggcattg gctcaaccga ggcaagcggt gagaaaatta atgtcaatca caaatacacc
     7261 gataaagaga ttgccgagcg ttggataaaa gatcttaaaa tagctgaaag gtgtatcaat
     7321 cgttatttta atggcgacaa aatgaacgaa aatcagtttt cggcgatggt atcagcagca
     7381 tttaatatgg gatgttacaa ccttagattt tatccaaatg aaaatggtaa atacattcag
     7441 acaacaatcc ataaatatgc tatggctaag gattttaaag cgatgtgtaa taggatacct
     7501 gattttaacc gctcgggagg taaagtgcta agaggtttga aaatccgccg agaaaaagaa
     7561 aaagccctgt gtctcgggca ggaggttaaa tgattgtcaa actctgccaa atgaccaata
     7621 agcatttcag cccgctaatg attgtgctgg tgctagttgg cgtattagcc aatttcgtgc
     7681 agtttcgcca aaacgaaagc ctgaaagcag acttacaggc acgcaccacg caaaaccaac
     7741 tgattcaagc cgataaccgc aatttagcca atcagcttga aaacagccaa atacagcttg
     7801 aggcgtatca aaaacaggtg gacgacctca atcagaaagt gttagccaaa atgaaacaag
     7861 cggagcaacg aagtaatgaa atccttaacg aactggaaaa gcataaaaag tgggctgatt
     7921 ctgccgtgcc tcctagcgtt ggcaagctgc tcaaccaacg aaagcgtgcg gtatcacacc
     7981 ctcaaatcga acccgataat ctgtccgaaa aggctggagt gccaaacacc ggcaaccgag
     8041 attaacacca acggtgatct tgtcaaagca ctagacaaaa gactcaacac cattgaggta
     8101 tgccaagtca tcatcgaatc gttcgagcat tgcatcgagc aatacaacaa aatcaaccaa
     8161 ggcgagtgat cgcctttttt aataaggacg actatgtcag accaaatcga ccgagccaac
     8221 gaattagccg aaaaagccag agaggcggca cttgcaaaaa ttctgcaaaa tcagaccgct
     8281 tgcaccagcc tttttgagtg tgaggattgc ggagagccaa tccccgaaaa acgccgagaa
     8341 atggttattg gttgcacccg ttgcattgag tgccaaacca tttacgagca taagcaaaaa
     8401 ggctaccgca gatgattaaa cctgatcgct tgcgagccgt gctcacacaa accattgata
     8461 tattccaagc taaccccgaa aagctgattt tgcaatatga caaaggcaaa attaaaagca
     8521 aagggagcca aagccattcg tttgaatacc actacgattt agagctaatc gtggtggatt
     8581 ttccgtatca ccccgacgtg ttgtttgtgc cagtgctaaa ttttgtgcga aatgaacaat
     8641 gggagctact gcaaaacccg gaactacaag acaaaatcga atttgaaatc gaccacaaca
     8701 atcacgagag ctacgatatt tatatccgcc tcccgcttac cgagcgggtg atcgtgaaag
     8761 agcaagacgg acacctaact gcaacccacg ccgatgaacc gaaccttgcc gatgtttcgc
     8821 catttacccg tttaaccgaa tatgaggttt acctcaaaga cgagctgatt tatcagtggg
     8881 cagagccaaa tgagtgaacc gatcgaacaa gtaaaaaccg cctttgatag cttactgaat
     8941 aatatcagca aacctcgcag acggttgatg tatcaacaaa tcgggcgaga gcttgaccga
     9001 agccaacgca gacggattaa agcacaacaa aacccagacg gctcggcata cgagccacgc
     9061 aaaaagccga agaaaggggt aaaaagcaaa attaaatcgg gcaagatgtt tgacaaaatt
     9121 acccagccac gttttatgcg gctacgcctt gaaagcgaag gtgtcagctt aggttatgag
     9181 ggtggtgatg cggtcatcgc ccgaatccac caacaaggct taatcggacg agtgcggaaa
     9241 gattgggatt taaaagtgaa atatgccagc cgtgagttgc taggttttac tgatgacgat
     9301 ctgcagatga ttgaggatta tatgattaat attttggctg gtagctaaat gatgaataga
     9361 aacttttgca aaaaagcggt caattttgac cgcttgagtt attgattaac gctttgtcta
     9421 tctgaatgat accaacaaag aaaccctaaa atagctgaaa aaatcaatgc tatcaaaata
     9481 aaagtattga gtggtaacaa tacataaagt gagatcagaa taacgataat agagagccca
     9541 accatatttg ccaataatga aagcaggctt aaaaatactg ttgtaactac accaaaaatg
     9601 gcagcaaagg ttgtaaaacc tattgccaat aatgcaccga aaccaattaa cccaagtaag
     9661 ataaataaga tttccataga ttttccctta gccatactac aattaaggtt ataatatgag
     9721 caataattta aaaattcaag tagttctttc tgcgttagat aaattaaccg caccatttaa
     9781 gagtgtggta aaatagacgg agaaattatc tcaatcttta aaagtacaga gagatgaatt
     9841 aaaagcattg gaaaaaacac aaagtaaaat gcaatcattt aaaaaaatgg acgatagcat
     9901 taagaaatcc aatgaaacta tactcaaaca gacaaagcat ttagataatc tcaaaaataa
     9961 aatcagc
//
LOCUS       DANLZC010000095         9804 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.095, whole genome shotgun sequence.
ACCESSION   DANLZC010000095 DANLZC010000000
VERSION     DANLZC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9804
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.095"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..728
                     /locus_tag="PXE44_001787"
     CDS             <1..728
                     /locus_tag="PXE44_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5589276.1"
                     /translation="SAFAKESLLERINNKGTITVGTEGTYAPYTYHDASGKLTGYDVE
                     VTRAVAEKLDVKVEFKETQWDAMLAGLKGERFDLVANQVALTSPERRATFDKSESYSW
                     DGAALLVREDETKIKTPADVKGVKTAQTLSSNYGEIARENGADIVPIDGMAQGLLLIQ
                     QKRADATFNSHLSLLDYLKKNPNSGLKIVWEKEEKVGAGLIANKGNDEALAKISKAIV
                     ELREDGTLKKLGEQFFGKDISVQ"
     gene            811..1527
                     /locus_tag="PXE44_001788"
     CDS             811..1527
                     /locus_tag="PXE44_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL5589277.1"
                     /translation="MLNDLLLSIPFMTQARVDLVLSGFWPMVQAAFLVSIPLAIASFI
                     IGMLIAVAVALVRISPATGFFHRLLLWLVKGYISIIRGTPMLVQICIVFYGLPAIGIF
                     IDPIPAAIIGFSLNIGAYGSETIRAAISSVPKGQWEAGYTIGMTYMQTFRRIIAPQAF
                     RVAVPPLSNTFIGLFKDTSLASVVTVTEMFRVAQQVANMSYDFLPVYIEAGLIYWCFC
                     WVLFLIQARLEVRFNRFVAK"
     gene            1537..2289
                     /locus_tag="PXE44_001789"
     CDS             1537..2289
                     /locus_tag="PXE44_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL5589278.1"
                     /translation="MLKIRNIHKTFGNNAILRGIDLDIQKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEQGTLEFSGNAPLKIDFAVKQSKKEILALRRKSGMVFQNYNLFPHKTAL
                     ENVMEGPVFVQQQAVEIAQKNAKALLEKVGLADKAELYPFQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNTMKELANEGWTMVVVTHEIKFALDVADVVVVMDG
                     GEIVEQGSPQQLFNHPQHERTKRFLNQIRADK"
     gene            complement(2505..3038)
                     /locus_tag="PXE44_001790"
     CDS             complement(2505..3038)
                     /locus_tag="PXE44_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL5589279.1"
                     /translation="MEFLAPVISLIVGFLSFVLVLRTWLQFCRVDPRLPISQTLLKIT
                     EPMVSPVGKLIPTVKNICFPALLIAAALVTLQFILFGVDLPRAVLIGLLSVVKTFGQI
                     LFFTTLIRALMSWVTQGNHPLDYTVAQITEPVLGFIRKLLPRTGMLDFSVMVLGFGLL
                     LLNSLFYQIFGGLWAAA"
     gene            complement(3144..3485)
                     /locus_tag="PXE44_001791"
     CDS             complement(3144..3485)
                     /locus_tag="PXE44_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDL5589280.1"
                     /translation="MAIQRNARQRKKMHLGEYQELGFLIKFTFAEGTGIDQIDETVDR
                     FIDEVIRPNGLALEANGYLNWEGLVCLEKLGKCDESHRQLVKTWLETNGLKEVQVSEL
                     FDIWWDYPAQA"
     gene            complement(3509..4258)
                     /gene="trmB"
                     /locus_tag="PXE44_001792"
     CDS             complement(3509..4258)
                     /gene="trmB"
                     /locus_tag="PXE44_001792"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL5589281.1"
                     /translation="MSEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQ
                     RDMMNNNWANLGLDYQPMPFNFEQIFGNNNPVVLEIGFGMGKSLVEMAEQNPNRNYIG
                     IEVHTPGVGACIAYALEKGVKNLRVICHDATEILRDSIADGTLGGLQLYFPDPWQKAK
                     HHKRRIVQPEFISRVIRKLSPNGFIHFATDWENYAEHMLEVLRQFESELQNTSATNDF
                     IPRPNFRPLTKFEERGHRLGHGVWDLYFTKK"
     gene            complement(4268..5218)
                     /locus_tag="PXE44_001793"
     CDS             complement(4268..5218)
                     /locus_tag="PXE44_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL5589282.1"
                     /translation="MNSRILATGSYLPTKVRTNADLEKMVDTSDEWIVTRSGIRERRI
                     AEPDETVATMGAKAAQQALEMAGIEANDIDLIVVGTTTNSHAYPSAACQIQGLLQIED
                     AIAFDVAAACTGFVYALTVADQFVRNGQVKKALVIGSDINSRMLDETDRSTVVLFGDG
                     AGAVILEASEELGIISTHLHASPDLENNLTLRSQKRGEERSGFIEMQGNATFKIAVNQ
                     LSSVVEETLEANNLQKSDLDWLIPHQANLRIITATAKKLEMEMDQVVVTLDKYANTSG
                     ATVPVALDEAVRDGRIQRGQLLLLEAFGGGWTWGSVLVKF"
     gene            complement(5364..6383)
                     /gene="hemB"
                     /locus_tag="PXE44_001794"
     CDS             complement(5364..6383)
                     /gene="hemB"
                     /locus_tag="PXE44_001794"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDL5589283.1"
                     /translation="MQVIQSQYPLRRMRRLRKHDFSRRLVAENQLTVNDLIYPVFVIE
                     GENQRVQVPSMPGVERLTIDQLLVEAAELVKYGVPAVALFPVVGDVKKSLMAEEAYNP
                     QGLAQRAVRALKAQFPELGVITDVALDPFTTHGQDGIIDETGYVLNDVTTEVLVKQAL
                     SHAEAGADIVAPSDMMDGRIGKIREALEQNGFINTQIMAYSAKYASNYYGPFRDAVGS
                     AGNLKGGNKYTYQVDPANGNEGLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKENFGV
                     PTFAYQVSGEYAMHMAAIQNGWLKERECIMEGLLCFKRAGADGILTYFAKRVAKWLYE
                     EAQAK"
     gene            complement(6464..7219)
                     /gene="tatC"
                     /locus_tag="PXE44_001795"
     CDS             complement(6464..7219)
                     /gene="tatC"
                     /locus_tag="PXE44_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDL5589284.1"
                     /translation="MSQVDESQPLITHLIELRNRLLRSFICVLLVFCGLVYWANDIYT
                     LLATPLTDNLPDGATMIATNVATPFFTPIKLTIVVSVFLSVPFILYQIWAFVAPALYK
                     QEKRLVYPLLVSSTLLFYCGVAFAYYVVFPLVFGFLTSTAPEGVTMATDISSYLDFVL
                     TIFMAFGICFEVPVAIILLCWAGVTTPEDLKAKRPYIIVAAFVIGMLLTPPDIFSQTL
                     LAIPMCLLFEVGVLFARFYRPKDDDEPSEQVAQ"
     gene            complement(7206..7850)
                     /gene="tatB"
                     /locus_tag="PXE44_001796"
     CDS             complement(7206..7850)
                     /gene="tatB"
                     /locus_tag="PXE44_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDL5589285.1"
                     /translation="MFDIGFSELVLIMIVGLVVLGPKRMPIAIRTVMGWVSTIRGLAA
                     NVQNELAQELKLQELKESIKKAEELNLSSLSPELNKTVEDLKMSATQLQDSLNSAKNE
                     LEKTAAESSTIPKLTDEQVAEIQQKIEQESQVLEAQELQKNAQNKPLEVDSLSEQLNV
                     TRTEKPLSADELAEQAEMDESLLTLESYYPPDDDLATPQSSESAQKSEANNVAS"
     gene            complement(7854..8075)
                     /gene="tatA"
                     /locus_tag="PXE44_001797"
     CDS             complement(7854..8075)
                     /gene="tatA"
                     /locus_tag="PXE44_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDL5589286.1"
                     /translation="MGGISIWQLLIVVAIIVLLFGTKKLRTLGSDLGESVKGFKKAMA
                     DEKKDAEFEKVEAKDAISAEQKAKEKEQA"
     gene            complement(8186..>9804)
                     /gene="ubiB"
                     /locus_tag="PXE44_001798"
     CDS             complement(8186..>9804)
                     /gene="ubiB"
                     /locus_tag="PXE44_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDL5589287.1"
                     /translation="RFYHIIRTFLRYGIDEAIPNLPITRKIKLGRKALFWVKNQHSDK
                     PYGVRLRLALQELGPVWIKLGQMLATRRDLFAPELADQLALLQDNVEPFDGKIARQII
                     EQALGGKLETWFENFDETALASASIAQVHTAKFNQNQPLAGKDVVLKVIRPGIEPVIK
                     ADLELMYEIAHFIPKLSNDGKRLRAVEVVREYEKTILDELDLRKEMHNAIRLRANFEN
                     SEMLYVPEMYQDFCHKNLIAMERIYGIPVSNIAELEANSTDMKLLAERGVQVFFTQVF
                     RDSFFHADMHAGNIFVNREHPENPQYIGIDCGIVGTLNHNDKRYLAESFVAFFNRDYR
                     RVALMHVESGWTPPDTDIDKFEQAFREVCEPIFAKPLSEISFGHVLLNLFNVAREFNM
                     EVQPQLVLLQKTLLYIEGLGRQVYPQLDLWQTAKPFLQNWLNEQVGVKAMVRDIKQRL
                     PQYREHFAEFPEALFKALQEQKQINFRLAEINESLKAQNSNANSRFLVLSISAIVICG
                     AFWKFESLPIWLSVPLLTLFPLVLILSQRK"
BASE COUNT         2789 a         2249 c         1953 g         2813 t
ORIGIN      
        1 tttctgcatt tgctaaagag agcctacttg agcgtataaa caacaaaggg actattactg
       61 tgggtacgga aggaacttat gccccttaca cctatcacga tgcgagcggt aaattaaccg
      121 gttacgatgt agaagtaacc cgtgcggtgg ctgaaaaatt agacgtaaaa gtggaattta
      181 aagagacaca atgggacgct atgttagccg gtttaaaagg tgagcgtttt gacttagtgg
      241 caaaccaagt ggctttaacc agcccggaac gtcgagcgac atttgataaa tccgaatctt
      301 acagctggga cggtgccgcc ttattagtgc gtgaagatga aactaaaatc aaaacccctg
      361 ctgatgtaaa aggcgtgaaa actgcccaaa ccttgagctc aaactatggc gagattgccc
      421 gtgaaaacgg tgcagacatc gtgccaattg acggtatggc tcaaggctta ctgttaattc
      481 aacaaaaacg cgccgatgca actttcaata gccacttatc gctattagat tacttgaaaa
      541 agaacccgaa ttccggctta aaaattgtgt gggaaaaaga agaaaaagtg ggtgcaggct
      601 taattgcgaa taaaggcaat gacgaagcat tagcgaaaat cagcaaagca atcgtagaat
      661 tacgtgaaga cggcacgtta aaaaaattgg gcgagcagtt cttcggtaaa gatattagtg
      721 tacaataatc catattggat taggcttggg gcgaattcgc cccttttatt tttaaatctc
      781 cctcaaaacg ggagatttta ggtttttcag atgttaaacg atctacttct ctcaatcccc
      841 tttatgaccc aagctcgggt cgatttagtg ctaagcggtt tttggccgat ggtacaagcg
      901 gcatttttag tctctattcc gctggcaatc gcctcattta ttatcggtat gttaattgcc
      961 gttgcagttg cattagtgcg aattagccct gcaacaggct tttttcaccg cttgctactg
     1021 tggctggtaa aaggttatat ctcgattatt cgcggcacgc cgatgttggt tcagatctgt
     1081 attgtgtttt atggtttgcc tgcaatcggc atttttatcg atccgattcc ggcggcaatt
     1141 atcgggtttt cactcaatat cggggcttat ggctcggaaa cgatccgtgc tgcgatctcc
     1201 tccgtgccaa aaggacaatg ggaagccggt tatactatcg gtatgactta tatgcaaact
     1261 tttcgccgta tcattgcccc gcaagcgttt cgagttgctg ttccgccact tagcaacacc
     1321 tttatcggtc tgtttaaaga tacctctctt gcctccgttg ttaccgtaac cgaaatgttc
     1381 cgggttgctc aacaggtagc aaatatgtct tatgacttcc tacctgttta tattgaagcc
     1441 ggtttgattt attggtgctt ctgctgggtg ctatttttaa ttcaggcgag attggaagtc
     1501 cgctttaacc gatttgtggc gaaataagga ggaattgtgc ttaaaattcg caatattcat
     1561 aaaacctttg gcaacaacgc tattctacgc ggcattgatt tagatattca aaaagggcaa
     1621 gtggtagtaa ttttaggccc ttccggttca ggcaaaacca cctttcttcg ttgcttaaat
     1681 gcactggaaa tgcccgagca aggcacattg gaatttagtg gtaatgctcc gctgaaaatt
     1741 gattttgccg taaaacagag caaaaaagag attttagcgc tacgccgcaa atccggtatg
     1801 gtgttccaaa actacaacct gttcccgcat aaaaccgcat tggaaaatgt gatggaagga
     1861 ccggtatttg tgcaacaaca agcggtcgaa attgctcaaa aaaatgcaaa agccctgtta
     1921 gaaaaagtcg gactggcgga taaagcagaa ctctacccgt ttcaactgtc aggcgggcaa
     1981 cagcaacggg ttggcattgc acgagcatta gcaattcagc cggagctgat gttatttgat
     2041 gaaccaactt cggcacttga tcctgagtta gtgcaagacg tgctaaatac gatgaaagaa
     2101 ctggcaaatg aaggctggac aatggtcgtg gttactcacg aaatcaaatt tgcccttgat
     2161 gttgccgatg tagtggtggt aatggacggc ggtgaaatcg ttgaacaagg ctcgccacag
     2221 caactgttta accacccaca acacgaacga actaaacgtt ttttaaatca aatcagagcg
     2281 gataaataag aaacgatagc gctagcgtcc acgctagtgc tatctaataa atggtacaag
     2341 cggttaatta tgtataaatt gttatactgt tttggcacga gcgtggacgc tcgcgccatc
     2401 gtacagtaaa atcagctaac aaggttcccc ataaaggata aataagacaa ggctccttaa
     2461 tcttttagat taaggagctt atttttagat tttagaaatc cgatttaagc cgcagcccat
     2521 agtccaccaa aaatttggta gaacaagcta tttagtagca ataagccgaa acctagcacc
     2581 attaccgaga aatctaacat tcccgttctt ggcaggagct tgcggataaa acctaacacc
     2641 ggttcggtaa tttgtgctac cgtgtagtct aatgggtggt tgccttgagt gacccagctc
     2701 attaatgcac gaatcaaggt ggtgaaaaat agaatttgtc cgaaggtttt gaccacactt
     2761 aataagccga ttaatactgc tctgggtaaa tctacgccaa acagaataaa ctgtaacgtt
     2821 accaaagcgg cggcaattaa tagtgccgga aagcaaatat ttttaacggt tggaatcagc
     2881 ttgccaaccg gtgaaaccat cggttcggta attttaagta aggtttgtga aatcggtaaa
     2941 cgaggatcaa cccgacaaaa ttgcagccaa gttcttagca ccagcacaaa acttaaaaag
     3001 ccaacaataa gcgaaataac aggagctaaa aattccatct ctttttctct ttctatacaa
     3061 aacaagcggt cgttttttag aaaaaatttg caaattttca gtaaaatctg accgcttgta
     3121 tcttattaaa ttaactaggc aaattaagct tgtgctgggt agtcccacca aatatcaaat
     3181 aattcgctca cttgaacctc ttttaagccg ttagtttcta accaagtttt cactaattgg
     3241 cggtgagatt catcacattt gcctaatttt tctaagcaga ctaaaccttc ccagtttaag
     3301 tagccgttcg cttcaagggc taaaccgttt ggtcgaatca cttcatcgat aaaacgatct
     3361 acggtttcat caatttggtc aatgcctgtg ccttcagcaa aggtgaattt aattaagaaa
     3421 cctaattctt ggtattcacc taagtgcatt tttttacgtt ggcgtgcgtt acgttgaata
     3481 gccatttttt ctctcctcta tgaataaatc atttctttgt aaaatataaa tcccacaccc
     3541 cgtgtcctaa acgatgccct cgctcttcaa atttggttag cgggcggaaa ttcggacgtg
     3601 gaataaaatc attagttgct gatgtatttt gcaactcgct ctcaaattgg cgtaatactt
     3661 caagcatatg ttctgcgtaa ttttcccagt cggtggcaaa atgaataaag ccgttagggc
     3721 ttaattttct gatcacacgg ctgataaatt caggctgcac aatacggcgt ttgtggtgtt
     3781 ttgctttttg ccaagggtct gggaagtaaa gctgtaagcc acctaatgta ccgtcagcaa
     3841 tgctgtctcg caaaatttcg gtggcatcgt ggcaaattac tcttaaattc ttcacgcctt
     3901 tttctaacgc ataagcgata caagccccca ccccgggcgt gtgcacctca atgccgatat
     3961 agttgcgatt cgggttttgc tctgccattt ccaccagcga ttttcccatt ccgaagccga
     4021 tttctaacac taccgggttg ttattgccga aaatctgctc aaaattaaag ggcattggct
     4081 ggtaatctaa gcctaaattt gcccagttgt tgttcatcat atcacgttga taatcgctca
     4141 aacgaccggt acgcaataca aaactccgca cttttcgtaa ataacgccca tcttcggtaa
     4201 actcggcttg ctcaacggtt ttacgttttt tatctgcaaa agtctgtttt tctgacattt
     4261 tcttgcctta aaacttcact aataccgaac cccaagtcca gccaccgccg aatgcttcaa
     4321 ggagtagtaa ttgcccacgt tgaattcgcc cgtcacgcac cgcttcatct aatgccaccg
     4381 gcactgttgc tccactggta ttggcatatt tatcaagggt aaccaccact tgatccattt
     4441 ccatctctaa ttttttcgcc gttgcggtga taatgcgtaa attcgcttgg tgcggaatca
     4501 gccaatctaa atcggatttt tgtagattat tcgcttctaa ggtttcttct accacgcttg
     4561 aaagttggtt tactgcaatt ttaaaggtgg cattgccctg catttcaata aagccggaac
     4621 gctcttcgcc acgcttttgg cttcttaagg ttaagttatt ttctaaatcc ggtgaggcgt
     4681 gcaagtgggt tgaaataata cccagctctt cactcgcctc taaaatcacc gccccagctc
     4741 cgtcaccaaa taatactact gtgctgcggt cggtttcgtc taacattctg gaattgatgt
     4801 ctgaaccaat aaccaatgcc tttttcactt gcccgttacg cacgaattga tcggcaacag
     4861 ttaaagcata cacaaatcct gtacaagccg ctgccacatc aaaagcaatc gcgtcttcaa
     4921 tctgaagcaa gccttgaatt tggcaagctg cacttgggta agcgtgggag ttggtagttg
     4981 ttcccaccac aattaaatca atgtcatttg cctcaattcc tgccatttcc agtgcttgtt
     5041 gagcggcttt tgcccccatt gttgccactg tttcgtcagg ctcggcaata cggcgttcac
     5101 gaatgccgga acgggttacg atccattcat cagaggtatc gaccattttt tctaaatcag
     5161 cattggtacg cacctttgtc ggtaaatagc ttcccgttgc taaaattcta ctattcatat
     5221 ttttattatt ctcaatcaat taaatgataa tccgttatta tagcttgaaa accaacaaat
     5281 atataggctt tacaagcggt catttttagc aaaaaagttg cattcacgaa cgattttgca
     5341 ctagaaccac cgcttgctag tatttatttc gcttgagcct cttcgtatag ccacttcgct
     5401 actcgcttgg cgaaataagt tagaataccg tctgctcctg cccgtttaaa gcagagtaat
     5461 ccctccataa tgcactcacg ctctttcagc cagccgtttt ggattgccgc catatgcatc
     5521 gcgtattcgc ccgaaacttg gtaggcgaag gttggcacgc caaaattctc tttcacacgc
     5581 cacaccatat ccaaataagg catacccggt ttcaccatca ccatatctgc cccttcttga
     5641 atatccatcg ccacttcgtg caagccttcg ttgccgttag ccggatcgac ttggtaagtg
     5701 tatttattac cgccttttaa gttgccggca gaacctactg catcacggaa cggtccgtaa
     5761 taattagacg catatttagc cgaatacgcc ataatttgcg tattgataaa accgttttgc
     5821 tcaagggctt cacgaatttt gccgattcga ccgtccatca tatcgctcgg ggcaacaata
     5881 tccgcccccg cttctgcgtg cgataacgcc tgttttacca atacttcagt agtcacatca
     5941 ttcagtacat agcctgtttc atcaataatg ccgtcttgtc cgtgcgtggt aaacgggtct
     6001 aacgccacat cagtaatcac gcctaattcc ggaaattgag cttttaaagc tcgcacggca
     6061 cgctgtgcca acccttgcgg gttataggcc tcttctgcca tcagtgattt tttcacatca
     6121 cccaccacag ggaaaagagc caccgcaggc acgccatatt taaccagttc tgcggcttca
     6181 actaaaagct ggtcgatagt taaacgctct acacccggca tagaaggcac ttgtactcgc
     6241 tgattttccc cttcgatgac aaaaacaggg taaatcaaat cattaacggt aagttgattt
     6301 tctgccacta agcggcggct gaaatcgtgc ttgcgtaaac gacgcatacg gcgtaaagga
     6361 tattgggatt gaattacttg catttttaat ttcctaattc ttaaaatacc tattcttgaa
     6421 ataagaaagg catagcaaac aggtgctatg ccttttcaaa atattactgt gcaacctgct
     6481 ctgatggttc atcatcatct ttggggcgat aaaaccgagc aaatagcaca ccaacttcaa
     6541 acaataaaca cattggaatt gccaataatg tttgcgagaa aatatctggt ggggtgagca
     6601 acataccaat tacaaatgct gccacaataa tataagggcg ttttgccttt aaatcttcgg
     6661 gtgtggttac gcctgcccaa cataacaaga taatcgcaac cggcacttca aagcagatcc
     6721 cgaatgccat aaaaatggtt aaaacgaaat caagatagct actaatatcc gttgccattg
     6781 ttaccccttc cggagcggta gaggttaaaa agccaaatac aagcggaaat acaacataat
     6841 aggcaaaagc gacaccacaa taaaacagca atgtgctaga taccaataat gggtaaacca
     6901 aacgcttttc ctgcttgtat aaagcagggg cgacaaacgc ccaaatttgg tacaaaataa
     6961 aaggaactga taaaaacacc gaaaccacaa tggttaattt aattggggta aaaaacggag
     7021 tggctacatt ggttgcaatc attgttgcac cgtctggtaa attgtccgtt aaaggtgttg
     7081 ccaataaggt gtaaatatca ttggcccagt aaaccaatcc gcaaaacacc aagagcacac
     7141 agataaaact acgcagtaat cggtttctca gctcaattaa atgtgtaatc agaggttgag
     7201 actcatcaac ttgcgacatt atttgcctcc gatttctgcg ctgattctga tgattgaggc
     7261 gtagctaaat catcatctgg cggatagtaa gattccaacg ttaaaagcga ttcgtccatt
     7321 tctgcttgtt cagccagctc atcagccgaa agtggctttt ctgtccgcgt aacattaagt
     7381 tgctcagata aactatccac ttcaagcggt ttattttgcg catttttttg caattcttgc
     7441 gcctctaaca cctggctttc ttgctcgatt ttttgctgaa tttctgcgac ttgctcatcg
     7501 gttaatttag gaattgtgct gctttccgca gccgtttttt ccaactcatt ttttgcagaa
     7561 ttaagcgaat cttgcaactg ggtggctgac attttcagat cttccaccgt tttatttaat
     7621 tctggcgaaa gggagcttaa attcaactct tctgcctttt taatgctctc ttttaattct
     7681 tgcagtttca gctcttgagc cagttcattt tgtacgtttg cggctaaacc tcgaattgta
     7741 ctcacccagc ccattacggt acgaatcgca atcggcatac gtttcgggcc tagcaccacc
     7801 aagcccacaa tcataatcag tactaattca gaaaaaccta tatcaaacac ggattaagcc
     7861 tgctctttct cttttgcttt ctgctctgct gaaattgcat cttttgcttc cactttttca
     7921 aattcagcat cttttttctc atctgccatt gcttttttga agccttttac agattcgccc
     7981 aaatccgaac ctaaagtacg taatttcttc gtaccgaaaa gtaatacgat gattgcaacc
     8041 acaatcagaa gctgccaaat actaataccg cccataaaaa ctcctttcat ttaataggga
     8101 atagataaaa taaaacgctg tttattatat gttactttga catataaaaa agtaattttg
     8161 caaaaaattt gtcaaaaaca accgcttatt tgcgttgact caaaatcaat accagtggaa
     8221 ataaagttaa taacggcaca cttagccata tcggcaaaga ttcaaatttc caaaatgcgc
     8281 cacagatcac aatcgctgaa attgacagca caagaaaacg gctatttgcg tttgaattct
     8341 gtgcttttaa actttcgtta atttccgcta aacggaaatt aatttgcttt tgctcttgta
     8401 aggctttaaa caatgcctcc ggaaactccg caaaatgctc ccgatattgc ggtaaacgtt
     8461 gcttaatgtc tcgaaccatc gctttcacac ctacctgctc attcagccag ttttgtaaaa
     8521 acggttttgc ggtttgccat aaatccaact gtggataaac ctgcctgccc aaaccttcaa
     8581 tataaagaag tgttttttgc aacaacacca actgcggctg cacttccata ttaaattctc
     8641 ttgccacatt aaacaggttg agcaaaacgt gtccgaatga aatttccgat aacggcttag
     8701 caaaaatcgg ctcacatacc tcacgaaagg cttgctcaaa tttatcaata tcggtatccg
     8761 gcggcgtcca gcccgattcc acgtgcatta aggcaacacg gcggtaatct cggttgaaaa
     8821 atgcgacaaa actttcggct aaatagcgtt tgtcattgtg gttcaacgtg ccgacaatcc
     8881 cgcagtcaat gccgatatat tgcggatttt ccgggtgttc tctgttcaca aaaatattgc
     8941 cggcgtgcat atcggcgtgg aaaaaactgt cacggaatac ttgggtaaaa aatacctgca
     9001 caccacgctc tgccagcaat ttcatatcgg tgctgttcgc ttccaactcg gcaatgttag
     9061 acactggaat gccataaatc cgctccatcg caatcaagtt tttatggcaa aaatcttgat
     9121 acatttcagg cacatacagc atttcgctat tttcaaaatt cgcccgaagc cgaatggcat
     9181 tgtgcatttc tttgcgtaaa tccagctcgt ctaaaatagt tttttcgtac tctcgcacca
     9241 cttcaaccgc ccgtagccgt ttgccgtcat tcgataattt cgggataaag tgagcaattt
     9301 catacatcaa ctctaaatcc gccttaatca ccggctcaat gcccgggcga atcactttta
     9361 acaccacatc tttgcctgca agcggttgat tttggttaaa ttttgccgtg tgaacctgtg
     9421 caatcgaagc agaagctaag gcagtttcat caaagttttc aaaccaagtt tcaagctttc
     9481 cgcccaaagc ctgctcgata atttgacgag cgattttgcc gtcaaacggt tcaacattat
     9541 cttgtagcaa ggcgagctgg tcggcaagct ccggtgcaaa cagatcacgt ctggtagcta
     9601 acatttgccc gagcttgatc caaaccggtc caagctcttg caaagccaaa cgtaaacgta
     9661 caccgtaagg tttgtctgaa tgttgattct tcacccaaaa caaagcctta cgccctagct
     9721 ttattttgcg ggtaattggc agattcggaa tggcttcatc aataccatag cgaagaaaag
     9781 tgcggataat atggtaaaag cggt
//
LOCUS       DANLZC010000096         9726 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.096, whole genome shotgun sequence.
ACCESSION   DANLZC010000096 DANLZC010000000
VERSION     DANLZC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.096"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            54..1499
                     /gene="aldA"
                     /locus_tag="PXE44_001799"
     CDS             54..1499
                     /gene="aldA"
                     /locus_tag="PXE44_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDL5589288.1"
                     /translation="MVKQHKMYINGEFVPALDGRTLAVYNPATEKAISEVPRGGADDA
                     KIAIDAAEAAQEAWERLPAIERGKYLRQIAVGIRERADEIARTISEEMGKTLSLATVE
                     VHFTADYLDYMAEWARRYEGEIIQSDRPNEHIFLYKKAIGVTTGILPWNFPFFLIARK
                     AAPALVTGNTIVIKPSEDAPNNAILFSEIVHQVGLPKGVFNVVTGYGNEVGPELSGNE
                     KIGMVSFTGSQFAGQKVMEAASKNIIKVNLELGGKAPAIVLDDADLELAATAVVNSRI
                     INSGQVCNCAERIYVQSSIKEQFVQKLVEKMQAVKYGNPLEQQDIDMGPMVNEKGLHK
                     VQEMVTHALHQGGNLVLGGNVVEGTGYYFEPTIIDNVDNSMDIMRHEIFGPVIPVATF
                     DTIDEAIRLANDCEYGLTSSVYTQNINKAMKVISRLKFGETYVNRENFEAMQGYHAGW
                     RKSGIGGADGKHGLEEYLQTQVVYLQFDDKV"
     gene            1755..2402
                     /locus_tag="PXE44_001800"
     CDS             1755..2402
                     /locus_tag="PXE44_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HDL5589289.1"
                     /translation="MSIFQQTNPNRRRYGLAVFIGIIAGIISAFVKWGAEHPFPPRSP
                     IDLFTAACPQPVLDAMNAAADQVAAKTIALQECSRAFLNPPHVFLRDYIGIDPTEAAF
                     TFADHAFNWIGVTHMIFSLVFAIGYCIVAEIFPKIKFWQGIGAGIIANICVHYITFPA
                     LGLTPPVAEWPLYEHISELVGHIFWFWTIEVIRRDLRNRITREPDAEVPLDQPFR"
     gene            complement(2535..3911)
                     /gene="trkA"
                     /locus_tag="PXE44_001801"
     CDS             complement(2535..3911)
                     /gene="trkA"
                     /locus_tag="PXE44_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDL5589290.1"
                     /translation="MKVIILGAGQVGSTLAENLVSEDNDIVLVDNDPTRLDKLKDKHD
                     LQVIRGEYASPQTLRDAGAGDADLLVAVTNSDEVNMIACQIAYTLFNVPTKIARIRSA
                     DYVRERERLFNDDVLPIDHIIAPEILVKEDILRLIHYPGALQITHFADELVSIVNVKA
                     YYGGPLVGYPISALRDHLPHIDARVVAIFRQDKAIVPQGSTIIEAGDEVFFICATQHI
                     RAVMSELQRLDRPHKRIMIVGGGSIGSSLARDLEDQYRVKIIERNPVRAEKLAEKLSK
                     TMVLTGDASDEELLFEEHIENIDLFLAVTSDDEANIMSALLAKRLGAKKVIILVQRPA
                     YLHLIQGGTIDIAISPQQATISALASYVRKGDILQVSSLKLGVAGAVEIIAHGDATTS
                     KVVGRQIRELKLPQGAIVGAVVRGEEVIIAHKSTMIAENDRVIIFVNDKKQIDEIEKL
                     FQLGVFFL"
     gene            complement(3911..5242)
                     /gene="rsmB"
                     /locus_tag="PXE44_001802"
     CDS             complement(3911..5242)
                     /gene="rsmB"
                     /locus_tag="PXE44_001802"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL5589291.1"
                     /translation="MKKQSFKALSSRAISATIILQVLDQGKSLSTLIPDAQKQLEPKD
                     LPLVQEITFGVCRVLPRLESIIKLLVEKPLKGKTRLVHCLLLVGLYQLLYMRVPAHAA
                     VDEVVNATKTLKLDSFRALTNGVLRRFLREQEEILAKVDKHWQTLHPEWLVNKLKKAY
                     PNWREIVEANNQRPPMWIRVNQQHIKTNDYAALISELVEPEFAKNSENPTACVPKCAL
                     LLERAVNVNQLPHFEQGWATVQDAHAQWSAELLGAENGETVLDACAAPGGKTTHILEK
                     APKAKVMALDIEESRLSRVRENLVRLGQIAQVICGDASKPEEWLEEGVLFDRILLDAP
                     CSATGVIRRHPDIKWLRKEGDIAELATLQGEILKALWQRLKPNGILLYATCSVLPQEN
                     SEQIQRFLQDTPNAKQLEIDFNGEKVLEKQFFPQPNGGDGFFYAKLQKVAE"
     gene            complement(5294..5719)
                     /locus_tag="PXE44_001803"
     CDS             complement(5294..5719)
                     /locus_tag="PXE44_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL5589292.1"
                     /translation="MYNKLLIAIDLADLKSAKYVVDTALQMTAKNPNAVYRVVSIIEP
                     VDNSLISAFLPKNFDKEVVAEANQKLHEFTKNHFPEGSKVQHIVAYGTIYEEICRIAD
                     EKSVDLIMMLASSKPNAKGLSSNTVKVARNTTKPILVLR"
     gene            complement(5812..8460)
                     /locus_tag="PXE44_001804"
     CDS             complement(5812..8460)
                     /locus_tag="PXE44_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HDL5589293.1"
                     /translation="MSVESKVDYDDLQDMVASNDSGGRTPSGLAKKAIVYVAILWSVF
                     QIYYASPLPFVFQEWLTHAGINLNVVVDDTKARSIHLAFAMFLAFLSYPAFASSPKHH
                     IPKIDWVFAIVGAGLALYYIFFYEGLVKRFGAPNTQDIIAGCLGILLVLEATRRSLGL
                     PLVIIAIVFLIYNFFGQYFPADWIVSHRSGSLSQIINQQWITTEGVFGVALGVSTKYV
                     FLFVLFGALLDKAGAGNYFIKTAFAYLGHLRGGPAKAAVVSSALTGLISGSSIANVVT
                     TGTFTIPMMKRVGFTKEKAGAVEVASSVNGQIMPPVMGAAAFLMIEYVNMPYSQLITH
                     AFLPALISYIALVYIVHLEACKMNLQGLPRAEEPSPILVFLLRTVASILVIVGLAIGV
                     YYGLGWIRDVAEDYAFMIVCAFLGLWYLLAIRRVASYPDLEADDPNSEIVSLPKRKPT
                     VNAGLHYLIPVVVLLWCLMVEMLSPGLSAFWGTITLIFIQLTQRPLLNFFRKESVTKE
                     IIKQGVRDVVDSLEAGGRNMTGIALATATAGIIVGVVALTGFGVQLSSVIELLSMGNI
                     VLMLILVAVFSLILGMGLPTTANYIVVSSLMATVIMEVGRQNGLIVPLIAVHLFVFYF
                     GIMADVTPPVGLASFAAAAISGGSPINTGMVAFKYSLRTVILPFLFIFNTDLLLIDLR
                     YPGHGWVVFITATVGVLAFTSATMQYIVTKNKWWETALLVVAAFALFRPGFFMDRIYP
                     SERHIEPVQFEEQLLASKVGQNVTLKVEGLNPYGKEIEFYAQLPVPEGATGEERLKNL
                     GLTLIQTDEKIKVDGVETPKVVIDMVEMDSPAAKAGLNWDQTIHYIAVPQDAPSKDWI
                     FIPALLLVFGIVANQRRRVKKQCVIA"
     gene            complement(8662..9624)
                     /locus_tag="PXE44_001805"
     CDS             complement(8662..9624)
                     /locus_tag="PXE44_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HDL5589294.1"
                     /translation="MKKLVKLSFLASLVMGTASVQAAETFVTIGTGGQTGVYYVVGQS
                     ICQLVNRDSAKTGLKCNAPSTGASVANLNAIAAKEMDMGIAQSDWQYHAYNGSSSFEG
                     KKNDKIRAIFSLHPEPFTVMARTDSGIKSFDDLKAKRVNVGDPGSGTRATMNVILAAK
                     GWTDKDFKVASELKPAEMASVMCDNNLDAITYNVGHPNGALKEAAASCDAKLVPVTGP
                     EIDKLVADHSYYAKATIPGGLYKGSDEPTETFGVYATLVTSADVDADKVYNVTKAVFD
                     NFDRFKRLHPAFEHLKQEEMIKNALSAPLHEGAIRYYKEKGWIK"
BASE COUNT         2840 a         2192 c         1951 g         2743 t
ORIGIN      
        1 agttaccgaa tgatagtcat caacttccac tgtggataaa aaaggagcaa ctcatggtga
       61 aacaacataa aatgtatatt aatggtgaat ttgtgcctgc gttagatggt cgcactttag
      121 cagtctataa ccctgccacc gaaaaagcta tctccgaagt ccctcgtggc ggagctgatg
      181 atgccaaaat cgctatcgat gctgctgaag ccgcccaaga agcttgggaa cgtttaccag
      241 cgattgaacg aggaaaatat ttacgtcaaa ttgctgtggg gattcgtgag cgtgccgatg
      301 aaattgctcg cactatttcc gaagaaatgg gtaaaactct gtccttagca accgttgagg
      361 tacattttac cgctgactac ttagactata tggcagaatg ggcgagacgt tatgaagggg
      421 aaattatcca aagtgatcgc ccaaatgaac atattttcct atacaaaaaa gcgattggcg
      481 tcaccaccgg cattttacct tggaacttcc cgttcttcct cattgcccgt aaagcggctc
      541 cggcattagt aacaggtaat actattgtga ttaaaccgag tgaagatgca cctaataatg
      601 cgattctctt ttcggaaatc gtgcatcaag tcggtttacc taagggggta tttaatgtcg
      661 tcaccggcta tggcaatgaa gtcgggccgg aactttcagg caatgaaaaa atcggtatgg
      721 tttcctttac cggtagccag tttgccggac aaaaagtgat ggaagccgcc agcaagaata
      781 tcattaaagt caatttggaa ttgggcggaa aagcaccggc tatcgtatta gacgatgcag
      841 atcttgagct tgctgctacc gctgtagtaa attctcgaat catcaattca ggacaggttt
      901 gtaactgtgc agaacgcatt tatgtacaga gttcaattaa agagcaattt gtccaaaaat
      961 tggtagagaa aatgcaagcg gtgaaatatg gtaatccatt agaacaacaa gacattgata
     1021 tggggccgat ggtaaatgaa aaaggcttgc ataaagtgca ggaaatggtg acacacgcac
     1081 tgcatcaagg aggtaatctg gtattaggtg gtaacgtggt tgaaggaaca ggctactact
     1141 ttgagccaac tattatcgac aatgtagata actctatgga tattatgcgt cacgaaattt
     1201 ttggtccggt cattccggtt gcgacatttg ataccattga tgaagcgatt cgcttagcca
     1261 atgattgtga atatggctta acctcttcgg tttacacgca aaatatcaat aaagcgatga
     1321 aagtaatttc ccgcttaaaa ttcggagaaa cttatgtaaa ccgtgaaaac tttgaagcaa
     1381 tgcaaggcta ccacgccggt tggcgtaaat cgggtattgg cggtgctgac ggtaagcacg
     1441 gcttagagga atacctacaa actcaggtgg tttatctgca atttgatgat aaagtataga
     1501 ttcttctaaa aactcccact tatcaaaata agcgggagtt ttcttttagg ttaaccttgt
     1561 atcaaaattg caaacaaaaa tattctaatg ttaagtaaat gtttaataat tatttatggt
     1621 taactctttt tggctaatta atttgcaatt taaattaatt gagtgcagaa tgccattagc
     1681 cttttttgaa atcacattcc ataaaatggc ttgattaaca caacacaatt atcactcaaa
     1741 ttaggatttt cattatgagt attttccaac aaaccaatcc aaaccgccgt cgttatgggc
     1801 tagctgtatt tatcgggatt attgccggta ttatttcagc attcgtaaaa tggggggcag
     1861 aacatccttt cccacctcgt agccctatcg atctgtttac cgctgcttgc ccgcaacctg
     1921 tgttagatgc aatgaatgcc gcagcagacc aagtggctgc caaaaccatc gcattacaag
     1981 aatgttcacg tgcattttta aacccaccac acgttttctt acgtgattat attggtatcg
     2041 atccaactga agcagcattt acctttgctg accacgcgtt caactggatt ggtgtcaccc
     2101 atatgatttt ctcgttagtc tttgcgattg gttactgcat tgtggcggaa atcttcccaa
     2161 aaattaaatt ctggcaaggt atcggtgccg gtatcatcgc taatatttgt gttcactaca
     2221 tcaccttccc ggcattaggt ttaacaccac cggttgctga atggccgtta tatgaacaca
     2281 tttctgaatt agttggtcat attttctggt tctggaccat tgaggtgatt cgtcgcgatt
     2341 tacgtaaccg tattacgcgt gaacctgatg ccgaagtgcc gttagatcaa ccgttccgtt
     2401 aatttaaaat tagctcgatt tatacaaaag ccgcaatcac gttgcggctt tcttatttta
     2461 caagcggtca aattttcatg aaaatttacc aaacaaaaag cacattgttt catgtagcaa
     2521 tgcggagctt ggctttataa gaaaaataca ccaagttgga acagtttttc aatttcatcg
     2581 atctgtttct tatcgttcac aaaaataatc acacggtcat tttctgcaat catggtggat
     2641 ttgtgggcga taataacttc ttcgcctcgc accaccgccc caacaattgc cccttgcggt
     2701 agttttaatt cacggatttg tcgtccaacc acttttgaag tggttgcatc gccgtgagcg
     2761 ataatttcta ccgctcctgc aaccccaagt ttgagtgaag aaacttgtaa aatatcgcct
     2821 tttctaacgt aactagcgag agcagaaata gtagcttgtt gtggggaaat ggcaatatca
     2881 atagtgccac cttgaatcag gtgtaaatag gcagggcgtt gcactaaaat aatcactttt
     2941 ttcgccccaa gccgttttgc cagcagggcg gacataatat tcgcttcatc atcactggtg
     3001 acggcaagga ataaatcaat attctcaata tgctcctcaa acagcaactc ttcatcggag
     3061 gcatcaccgg taagcaccat cgttttagat agtttttctg ctaatttttc ggctcgaacc
     3121 ggattgcgtt cgataatttt tacccgatac tgatcttcta aatcccgagc taacgaagag
     3181 ccgatgctcc ctccaccgac aatcataatc cgcttatgtg ggcgatcaag ccgttgtagt
     3241 tcactcatta ctgctcgaat atgttgggtt gcacagatga aaaagacttc atcacctgcc
     3301 tcaataatag ttgaaccttg tggcacaatc gctttgtctt ggcggaaaat tgccacgacc
     3361 cgagcatcaa tatgcggtaa atgatcacgc agggctgaaa tcgggtaacc gaccaaaggc
     3421 ccaccgtaat aggcttttac attcacgata ctgactaact catcagcaaa atgagtaatt
     3481 tgcaacgccc ccggatagtg aataaggcgt aaaatatctt ctttgactaa aatttcaggg
     3541 gcaataatat ggtcaatagg caatacatca tcattaaaca accgctctcg ttctctcaca
     3601 taatcagcac tgcgaatacg ggcaattttg gtcggcacat taaataaagt gtaagcaatt
     3661 tggcaagcga tcatattcac ttcatcacta ttagttacag caactaatag atcagcatca
     3721 cctgcaccgg catcacgcaa ggtttgaggt gaagcatatt cgccccgaat cacctgtaaa
     3781 tcgtgtttat ctttgagctt gtctaaacga gttgggtcat tatccaccaa gacaatatca
     3841 ttgtcttcac tcaccaaatt ttctgccagc gtagaaccaa cttgtcctgc ccctaaaata
     3901 attactttca ttactctgcc actttttgta gttttgcgta gaagaaacca tcaccaccgt
     3961 ttggttgtgg gaagaactgt ttttctaaca ctttttcgcc gttaaaatcg atttcaagtt
     4021 gttttgcatt cggtgtatct tgtaaaaacc gttgaatttg ctcgctattt tcttgcggta
     4081 acaccgagca agtggcatat aacaaaatgc cgtttggctt taacctttgc cataacgcct
     4141 tgagaatctc accctgtaat gtggctaatt ctgcaatatc accctcttta cgcaaccatt
     4201 taatatcagg gtggcggcga atgactccgg ttgcggaaca aggggcatca agtaaaatgc
     4261 ggtcaaacag cacaccctct tcaagccatt cctcaggttt ggaagcatcg ccacaaatta
     4321 cctgtgcaat ctgccccagt cggactaagt tttcacgcac tcggcttaaa cggctctctt
     4381 caatatccaa cgccataacc tttgctttcg gtgctttttc taaaatatgg gtagttttgc
     4441 cacccggtgc ggcacaggca tctaaaacgg tttcaccatt ttctgccccg agcaactccg
     4501 ctgaccattg tgcgtgagca tcttgcacgg ttgcccaacc ttgctcgaaa tgtggcagtt
     4561 ggttcacatt taccgctctc tccagcaata aagcacactt tggcacgcaa gcggtcggat
     4621 tttctgaatt ttttgcaaat tcaggctcaa ccagctcgct aatcaacgcc gcataatcat
     4681 tggtcttgat atgttgttga ttgactcgaa tccacattgg tgggcgttgg ttgtttgctt
     4741 caacaatttc tcgccagttc ggataggctt ttttaagttt attgaccaac cattcagggt
     4801 ggagcgtttg ccaatgttta tctaccttcg ctaagatttc ttcttgctct cgcagaaaac
     4861 ggcgtagcac accgttagtc aaggctcgga agctatccag ttttagcgtt ttagttgcat
     4921 tcactacctc atcaaccgca gcgtgtgccg gcactcgcat ataaagcagt tggtataaac
     4981 ccactaacag taagcaatgc accaaacggg ttttgccttt cagcggtttt tcaactaata
     5041 atttgatgat cgattccaag cgaggcagca cacggcacac tccgaaggtg atttcttgca
     5101 ctaagggtaa atcttttggt tcaagctgtt tttgagcgtc aggaatcaag gtggaaagtg
     5161 atttaccttg atctaacacc tgtaaaataa tggttgcact tattgctcta gaagaaagtg
     5221 ctttgaacga ttgttttttc ataaataaaa atgccattcc gacagaatgg cactattgta
     5281 actgaatgcg ggattatctt aaaactaaaa ttggcttggt ggtgttgcga gccactttaa
     5341 ccgtattaga gcttaagccc ttcgcatttg gtttgctgct tgccagcatc ataattaaat
     5401 ccaccgactt ttcatcggca atacggcaaa tttcttcata aatcgtacca tacgccacaa
     5461 tgtgctgcac ttttgaacct tcaggaaaat ggtttttggt aaattcgtgc aatttttggt
     5521 ttgcttcagc caccacttct ttatcgaaat ttttcggcaa gaatgcagaa attaagctgt
     5581 tatccaccgg ctcaataata gacacaaccc gataaaccgc attcggattt tttgccgtca
     5641 tttgtaatgc cgtatctacc acatatttag cactttttag gtcggctaaa tcaatcgcaa
     5701 taagtaattt gttatacata tttattccgt aggggcaggt cctgtgcctg cccgaatatt
     5761 aactaaaata gcggacgggc acaggacccg tccctacata gcatataaat actatgcaat
     5821 aacacactgt tttttcactc tacggcgttg gtttgccaca ataccaaata ccaacaatag
     5881 agcaggaatg aagatccaat ctttagaagg agcatcttgc ggtacggcaa tgtagtgaat
     5941 ggtttgatcc cagtttaagc ctgctttagc tgccggagaa tccatttcta ccatatcaat
     6001 caccactttt ggtgtttcta cgccatcaac tttgattttt tcatcggttt gaattagcgt
     6061 taagcctaag tttttcaaac gctcttcgcc ggttgctcct tccggtacag gtaattgagc
     6121 gtagaactca atttctttac cgtaagggtt taatccctct acttttaaag taacattttg
     6181 acctactttg cttgcaagca actgttcttc aaactgcacc ggctcgatat gacgttcaga
     6241 cggataaatt ctgtccataa agaagcccgg acggaacaag gcgaaagcgg ctacaaccag
     6301 caacgccgtt tcccaccatt tgtttttagt cacaatatat tgcattgtag cagaggtaaa
     6361 ggcgagaacc ccaacagttg cggtaataaa cacaacccag ccgtgccccg gataacgcag
     6421 atcaattaat aataaatcgg tattaaaaat aaataagaac ggtaaaatga cggttcttaa
     6481 actatattta aatgccacca taccggtatt aatcgggcta ccgcctgaaa ttgctgccgc
     6541 agcgaacgag gctaaaccaa caggtggagt cacatctgcc ataatcccaa aatagaatac
     6601 gaataaatgc accgcaatca acggcacgat taacccgttt tgacgaccga cttccatgat
     6661 aaccgttgcc attaatgatg acaccacaat gtagtttgca gttgtcggta agcccattcc
     6721 caaaattaag ctgaatacgg ctaccaagat caacattagc acaatgttgc ccattgatag
     6781 gagttcaatc acacttgaaa gctgaacccc aaagcccgtt agtgccacaa cgccgacaat
     6841 aatccctgct gttgcggtag cgagggcgat accggtcata ttacgaccgc cagcctcaag
     6901 gctatctacc acatctcgaa caccttgttt gataatctct ttggttacgc tctctttacg
     6961 gaagaaattc agtaacgggc gttgggttaa ttgaataaaa attaaggtaa tggttcccca
     7021 gaatgcggat aagcccggcg aaagcatttc caccattaag caccaaagta gcacaaccac
     7081 aggaataagg taatgtaaac ctgcatttac ggttggtttt ctttttggta gagaaacaat
     7141 ttctgagttt ggatcgtccg cttctaaatc agggtagctt gccacacggc gaatagccag
     7201 tagataccat aagcctaaga aagcacagac aatcataaac gcataatctt ctgccacatc
     7261 acgaatccaa cctaagccgt agtaaacgcc aatcgctaat cctacgatca ctaaaattga
     7321 agctacggtt cttagcaaga agactaaaat cgggcttggc tcttcggctc ttggtaagcc
     7381 ttgtaaattc atcttacaag cttctaaatg cacgatatac accaacgcaa tatacgaaat
     7441 taacgcaggt aagaaggcgt gagtaatcag ctggctatac ggcatattca catattcgat
     7501 catcaagaac gctgcagccc ccattaccgg tggcataatt tgaccgttta ctgatgaggc
     7561 aacttccacc gcaccggctt tttctttggt aaagccgacc cgtttcatca ttggaatagt
     7621 gaatgtacct gtcgtcacga cgtttgcaat cgaagagcct gaaatcaagc cggttaaagc
     7681 ggaagaaacc accgccgctt ttgccggacc accacgcaaa tgccctaaat aggcaaaggc
     7741 ggttttaata aagtagttac ctgctcctgc tttatcgagc aacgccccaa ataatacgaa
     7801 caggaacaca tatttggtcg aaaccccaag ggctacacca aacacgcctt cagtggtaat
     7861 ccattgttgg ttgataattt gagaaagcga acctgagcgg tggcttacaa tccaatctgc
     7921 cgggaaatat tgcccgaaga agttatagat caggaataca atcgcaataa tcacaagcgg
     7981 taagccaaga ctacggcggg tcgcttccag caccaataaa atccctaaac agccggcgat
     8041 aatatcttgc gtatttggcg ctccaaaacg tttcaccaaa ccttcataga agaaaatata
     8101 atacaacgct aacccagctc ctacgatggc aaacacccaa tctattttag gaatatgatg
     8161 tttaggcgag ctggcaaaag cagggtaaga taggaacgcc aagaacattg caaaggctaa
     8221 gtggatagaa cgggctttgg tgtcgtccac caccacattc aaattaatgc ctgcgtgagt
     8281 aagccattct tggaatacaa acggcaatgg agaagcataa taaatttgga acacagacca
     8341 aagaatggcg acatacacaa tggctttttt cgcaagaccg gacggggttc gccccccact
     8401 gtcattggag gcaaccatat cttgtaggtc atcgtaatca accttagatt caactgacat
     8461 aaagactcct caaaacgcaa gcggtcattt tcttattaaa ttttacaaat ttcctcaaaa
     8521 aacaaccgct tgctcgtgaa aacactgttg ataagttatc aattaaaggt gaaaatcgat
     8581 aacttatcaa cagtttcaaa aaagcaaaaa accagcacgc taaaaaacgt gctgattgaa
     8641 tcaaattaca gaaaggaaat attacttaat ccaacctttc tctttatagt aacgaattgc
     8701 accttcgtgt aacggagcag aaagggcgtt tttgatcatc tcttcttgtt ttaagtgttc
     8761 aaacgccgga tgtaaacgtt tgaaacggtc gaagttatca aataccgctt tggttacatt
     8821 gtacacttta tctgcatcca catcggcaga agtcactaac gttgcatata caccgaaggt
     8881 ttctgtcggc tcatccgaac ctttatataa accgcccgga atcgtagctt tggcataata
     8941 tgagtgatct gccactaatt tatcaatttc agggccggtt accggcacta atttcgcatc
     9001 gcaagaagcg gctgcttctt ttaacgcacc attcgggtga cctacgttat aagtgatggc
     9061 atctaagttg ttatcacaca ttacagatgc catttctgcc ggtttcaatt cagaagccac
     9121 tttgaaatct ttatccgtcc agccttttgc cgctaaaatc acgttcattg ttgcacgagt
     9181 accggaaccc gggtcgccca cgtttacacg ttttgctttt aaatcatcga acgatttaat
     9241 gcctgaatcg gtacgagcca ttactgtaaa cggttccggg tgaagcgaga aaatggcacg
     9301 aattttatcg tttttcttac cttcaaatga gcttgagccg ttataggcgt ggtattgcca
     9361 gtctgattgt gcaatcccca tatccatttc ttttgctgca atcgcattta agtttgcaac
     9421 cgatgcccct gttgaaggag cgttacattt taaacctgtt tttgctgaat cacggtttac
     9481 taattggcaa atagattgac ccacaacata ataaacaccg gtttgaccgc ctgttccgat
     9541 agtcacaaat gtttcagccg cttgaacaga tgctgttccc atcacaaggc ttgcaaggaa
     9601 agaaagtttg actaattttt tcatttttaa ctcctaaaat taaggatcag aaaacaaaga
     9661 tttattgttt tgagccactg ctcacactac aagagataca aaacttcttt gagttgcatt
     9721 tgcctc
//
LOCUS       DANLZC010000097         9614 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.097, whole genome shotgun sequence.
ACCESSION   DANLZC010000097 DANLZC010000000
VERSION     DANLZC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.097"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            61..1539
                     /gene="fruB"
                     /locus_tag="PXE44_001806"
     CDS             61..1539
                     /gene="fruB"
                     /locus_tag="PXE44_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDL5589295.1"
                     /translation="MLNLSAKNIRLNGSASNKEEVIRFVAAELVAKGNVAAGYEVGML
                     NRETQTSTFLGNGIAIPHGTLDTRDLVKETGVQIIQLPQGVEWGEGNTAYVVIGIAAK
                     SDEHLTLLRQLTTVLSDEEAAATLAKTQNLDEFIAVLSGKKNLPALSQELISLNIESS
                     SPITLSAINAAKLQEQGYVSQAFISDVVANKALHLGNNLFVVDSAKGNQANGIAIARN
                     TQGQTLLTVAETDNALQPLLTQLLKPEVRSQFAHFSAEQMLGLLKGESVASTPVTNVE
                     ASGNQVVGTFTIRNDNGLHARPAAVLVQTVKPFSSKISVENLDRNTAPASAKSAMKVV
                     ALGASQGHRLRFVAEGEDAQQAIEALQQAIINGLGESVSFVPAVADTIESIAVSAIPA
                     EETATAVDANTKEATFVIKNEHGLHARPSAVLVNEVKKFNAKIEVQNVDKNSPLVSAK
                     SLMKIVALGVTKGTTLRFVASGDDAEVALAAIGAAIEAGLGE"
     gene            1553..2497
                     /gene="fruK"
                     /locus_tag="PXE44_001807"
     CDS             1553..2497
                     /gene="fruK"
                     /locus_tag="PXE44_001807"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL5589296.1"
                     /translation="MRIATVTLNPAFDLVGKLQRIEIGEVNTVETLGLYPAGKGINVA
                     KVLADLGTKLSVTGFLGEENQGDFVNAFKHNDLADEFYRINGKTRINVKITETEADVT
                     DLNFLGFEISESDWEKFVTLSHQWESKFDLVAVCGSLPRGVTPEKFASWLESLHRQGL
                     KVVLDTSNAALTAGLTAHPWLVKPNRRELEVLVGHSLNSIEEIISAAKQLRSRGIENV
                     IISMGEQGSLWLNHEGVLQARPPRCENVVSTVGAGDSMVASLIYGLSQGWSKEQTLRF
                     ASATSALAVVQSNVGIADRAALDDIIKQTDITVISENH"
     gene            2523..4187
                     /locus_tag="PXE44_001808"
     CDS             2523..4187
                     /locus_tag="PXE44_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL5589297.1"
                     /translation="MNISFVFPQTLGNARRFLVNEMLSAAAKQQGHNVVAANQADFVV
                     LFDNAIPQEAAGKQGAILDLEQAFAQPEASLKQAVENAQTFANATATTAVSAVSSGVK
                     NIVAVTACPTGVAHTFMSAEAIENYAKAQGWNIKVETRGQVGAGNPISAEEVAAADLV
                     FVAADIDVDLEKFKGKPMYRTSTGLALKKTTQEFEKAFAQAKIYEGGVAKADNAEAPT
                     SEKKGLYKHLMTGVSHMLPLVVAGGLLIAISFMFGIEAFKDETIAGGLPKALMDIGGG
                     AAFHLMIAVFAGYVAFSIADRPGLAVGLIGGMLATTAGAGILGGIVAGFLAGYTVKFL
                     NGAIQLPASLTSLKPILILPLLGSAIVGLLMIYVINPPVKALMDALVEWLNTMGQTNA
                     IILGIILGAMMCTDMGGPVNKAAYTFGVGLIASQQYMPMAAVMAAGMVPPIGMAIATL
                     IARSKFNTNQRDAGKASFVLGLCFISEGALPFVAADPVRVIASSILGGATAGAISMAL
                     GITLQAPHGGLFVIPFVSQPLMYLAAIAIGSLVTGVVYAAIKQKAE"
     gene            4354..4872
                     /locus_tag="PXE44_001809"
     CDS             4354..4872
                     /locus_tag="PXE44_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HDL5589298.1"
                     /translation="MILLRGDTMQFIEFTDPDSGISYQYSEFTIANVAFIINFCSDAD
                     VISTLSALGKDITNYINTYSCCTIKFMAKEHLENSGSNIDIYAPAANHQFKRKEIIAL
                     QETLERLLFEHYVRFTPESYLFIAERDSLNRMYQRMCVPRCDFMQSFQVVYPLGVNQD
                     CFILITPKGNLK"
     gene            complement(5025..5888)
                     /gene="rarD"
                     /locus_tag="PXE44_001810"
     CDS             complement(5025..5888)
                     /gene="rarD"
                     /locus_tag="PXE44_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5589299.1"
                     /translation="MLKGILASLAANVLFGAVYYFAILLRPLEGEAMFGFRIVVVIPF
                     ILLAIILFKQQSAFADLLQKIRQNPPLVLVILLLALNTGVQLWLFLWAPNHGQALQVS
                     FGYLLLPIVAVALGKMVFKEHFTRLKWLSLLFAVIGVGSSLFFNSTISWATWVAGLGY
                     PLYITLRRYFQINTLATFFVELLLVLPVALYYLSQVEMTPIVAENPQIYFYLGLLGLV
                     SGSAFILYILSSNLLPINVLGLLGYVEPLVMLLISFAIGEKLDANSYILMVCLLVSIG
                     LLSWDSFRSKV"
     gene            6066..6650
                     /locus_tag="PXE44_001811"
     CDS             6066..6650
                     /locus_tag="PXE44_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDL5589300.1"
                     /translation="MKKIFWILLIAMLAVGGYSLTKYNDLMRAEEDINSVWGNVESAY
                     QRRADLIPNLVNTVKGQANFEKETLTSVIEARAKATAVTIDPSNATEEQMAKFQEAQQ
                     GVNSALSRLLVSVEKYPELKAHEAFLNLQAQLEGTENRINVERNNFNEKVKEYNKQVR
                     EFPTKLAAMIMGFKAKPQFKSEAGSDKAPTVNFN"
     gene            6732..7190
                     /locus_tag="PXE44_001812"
     CDS             6732..7190
                     /locus_tag="PXE44_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL5589301.1"
                     /translation="MGLFSFLSNKLPIDTQRIEQAIADLEQKTSAELRVVVERKAKIG
                     KTDNAAMLRASELFDELKMRETAEHNGVLIYLSFKPHYAAIIGDEGIHQKVDNDFWQK
                     IYATMCLDCQQGDYTQAICDAIKMVEAPLAHHFPYQADDKNELSNEVVVK"
     gene            7187..8044
                     /locus_tag="PXE44_001813"
     CDS             7187..8044
                     /locus_tag="PXE44_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL5589302.1"
                     /translation="MMKSLCKFLSIFNRLLLVCLALFSLNATAVTYPEPPNPFYYITD
                     YTKNTLSQQEWRTLEDALIANCAKTSSQIAVVIIPSTEGEAVSTYAHTLFNKWGIGRS
                     KNNENNGILLLIAKNDRKLFIATGRGLEGALPDAIASSIIRNDITPFFKQEQYAAGIA
                     HGLSAIMAAINGEYAPYASYGEEMQEFDDIDGLLFFLFAGIVIFLIFRPRGNSTYISP
                     SSMDQLGQILRESQRRGGGFGGGLGGGFDRGSGGFGGGFGGGFGGSRGSGDSFGGGSS
                     GGGGAGGSW"
     gene            complement(8159..8638)
                     /gene="ispF"
                     /locus_tag="PXE44_001814"
     CDS             complement(8159..8638)
                     /gene="ispF"
                     /locus_tag="PXE44_001814"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDL5589303.1"
                     /translation="MIRIGHGFDVHAFGEDRPLMIAGVEVPYHTGFIAHSDGDVALHA
                     LTDALLGAAALGDIGKLFPDTDMQYKNADSRGLLIEAYRQVQEQGYQVGNVDVTIIAQ
                     APKMRPHIDKMRQVIADDLQCDISQVNVKATTTEKLGFTGRGEGIACEAVALLLKRA"
     gene            complement(8640..9323)
                     /gene="ispD"
                     /locus_tag="PXE44_001815"
     CDS             complement(8640..9323)
                     /gene="ispD"
                     /locus_tag="PXE44_001815"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDL5589304.1"
                     /translation="MKRKIIAVIPASGVGSRMQAEKPKQYLQLKGKTILEHTVSIFLE
                     HPHVEKVIVAVARKDPFYSTLELLKSPKIQLVFGGETRADSVLNALQEINTENAWALV
                     HDAARPCLKRSDLDKLLQINDPQGGILAIPAVDTMKRANGTKIAHTEDRSTLWHALTP
                     QFFPAQALKQALSSAFANKLQVTDEASAMELAGYQPQLVSGRSDNIKITRPEDLALAE
                     FYLTHNLEN"
     gene            complement(9341..>9614)
                     /gene="ftsB"
                     /locus_tag="PXE44_001816"
     CDS             complement(9341..>9614)
                     /gene="ftsB"
                     /locus_tag="PXE44_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDL5589305.1"
                     /translation="LLITFFALLLGFFQYSFWFGKNGWSDYQEVQEEIALLKVENTKL
                     TSRNKLIEAEIYDLKNGVDALEERARLEREMVKENEMFYRIVPKNK"
BASE COUNT         2886 a         2078 c         2014 g         2636 t
ORIGIN      
        1 tagagtaaac taaccataac attacagttt aaaaatcaac tttaaatcca aggagctaaa
       61 atgcttaatt tatcggcaaa aaacatccgt ttaaatggtt cagcaagcaa caaagaagaa
      121 gttatccgtt ttgttgccgc cgaattagtc gccaagggca atgtagcagc cggctatgaa
      181 gtcggtatgc ttaatcgtga aacccaaacc tctacttttt taggtaacgg aattgctatt
      241 ccccacggca cgcttgatac ccgtgattta gtcaaagaaa ccggcgtgca aattatccaa
      301 cttccgcaag gcgtggaatg gggcgaaggc aacactgctt atgtagttat cgggattgcg
      361 gctaaatcgg acgaacactt aaccttactc cgtcaactca ccactgtatt aagtgatgaa
      421 gaggctgctg ccacattagc gaaaacgcaa aatttagatg aatttattgc cgtattaagc
      481 ggtaagaaaa accttcctgc cttatctcaa gagctgattt ctctgaatat cgaatcttca
      541 agccctatca ctttatcagc cattaacgcc gcaaaactac aagagcaagg ctatgtttcc
      601 caagcattca tcagcgatgt ggttgccaac aaagcgttac atttaggcaa taatctattt
      661 gtggtggatt ccgccaaagg caaccaagct aacggtatcg ctattgcccg taatacgcaa
      721 ggtcaaaccc tactaactgt ggcagaaacc gacaatgctc tacagccact attaactcaa
      781 ttattaaaac cagaagtaag aagccagttt gctcatttct ctgcggagca aatgcttggt
      841 ttattaaaag gcgaaagcgt agcttcaacc cctgtaacca atgtcgaagc gagtggcaac
      901 caagtggttg gcacattcac cattcgtaac gacaacggct tacacgcccg cccggctgcg
      961 gtgttagtac aaacggtgaa accgttctcc tcaaaaatca gcgttgaaaa tttagaccgc
     1021 aacactgcac cggcaagtgc gaaaagtgca atgaaagtag tagcactagg ggcaagccaa
     1081 gggcatcgtt tacgctttgt ggcagaggga gaagacgccc aacaagcgat tgaggcatta
     1141 caacaagcaa ttattaacgg cttaggcgaa agtgtttcat tcgtaccggc ggtggcagat
     1201 acgattgaaa gcattgctgt ttcagcaatt cctgcggaag aaactgcgac tgctgtcgat
     1261 gccaatacga aagaagcaac ttttgtaatt aaaaacgaac acggtttaca cgctcgccca
     1321 agtgcggtgt tggtgaatga agtgaaaaaa ttcaacgcca aaattgaagt gcaaaatgtt
     1381 gataaaaatt caccgcttgt aagtgccaaa agcttaatga aaattgttgc ccttggcgta
     1441 accaaaggca cgacgctacg ctttgtggca agcggtgatg atgccgaagt agcacttgcg
     1501 gcaatcggtg ctgcgattga agcaggttta ggcgaataag cgaggaatca ttatgcgtat
     1561 tgcaaccgtt accctcaacc ctgcctttga tttagtcggc aaacttcaac gtattgaaat
     1621 cggcgaagtg aacaccgtag aaacccttgg tttataccct gccggcaaag gtattaacgt
     1681 tgctaaagtt ttagctgact taggcacaaa actttcggtt accggctttt taggcgaaga
     1741 aaatcaaggc gatttcgtaa atgcctttaa acacaatgat ttggctgatg aattttaccg
     1801 tattaacggt aaaacccgca ttaatgtcaa aatcactgaa accgaggctg atgtaactga
     1861 tctgaacttt ctcggctttg aaatttcgga aagcgattgg gaaaaattcg tcacattatc
     1921 tcatcagtgg gaaagcaaat ttgacttagt tgcagtgtgt ggatctctcc ctcgtggcgt
     1981 aacgccggaa aaatttgctt cttggttgga atctttacac cgccaaggct taaaagtggt
     2041 gttagatacc agcaatgcgg ctctcaccgc cggcttaacc gctcaccctt ggttagtaaa
     2101 accaaaccgc cgagagctgg aagtgctggt cgggcattca ctcaactcaa tcgaagagat
     2161 tattagtgca gctaaacaat tacgttcccg tggtattgaa aatgtaatta tatcaatggg
     2221 cgaacaaggc tctctttggc taaatcacga aggcgtgtta caagcccgcc caccacgttg
     2281 cgaaaatgta gttagcactg taggtgctgg tgattcaatg gttgccagct tgatctacgg
     2341 cttatctcaa ggttggagca aagagcagac cttgcgtttt gcctcagcta cttctgctct
     2401 agctgttgtt caaagtaacg ttgggattgc agaccgtgcg gcgttagacg atattattaa
     2461 acaaaccgac attaccgtga tttcagaaaa tcattaaacc tcaatcaaag gaacaaacca
     2521 atatgaatat ttcttttgta tttcctcaaa cactaggcaa cgcacgccgt ttcttagtga
     2581 atgaaatgtt atctgccgct gccaagcaac aaggacacaa tgttgtggcg gcaaaccaag
     2641 ctgattttgt ggtgttattc gataacgcaa ttccacaaga agctgccggc aagcaagggg
     2701 caattttgga cttagaacaa gcctttgctc aacctgaagc cagtctcaaa caagcggtcg
     2761 aaaatgcaca aacttttgca aatgctaccg caaccactgc ggtttcagcg gtttcatctg
     2821 gtgtgaaaaa tatcgttgct gttaccgctt gcccaactgg cgtggctcat acttttatgt
     2881 ctgcggaagc aatcgaaaat tatgccaaag cacagggttg gaacatcaag gttgaaaccc
     2941 gtggtcaagt aggagcagga aatccaattt ccgccgaaga agtcgcagcg gcagatttag
     3001 tctttgtagc tgccgatatt gatgtcgatt tggaaaaatt caaaggcaag ccgatgtatc
     3061 gcacctcaac cggtttagca ttgaagaaaa ccactcaaga atttgaaaaa gcctttgctc
     3121 aagcgaaaat ttacgaaggt ggtgtggcaa aagcggataa tgctgaagct ccaacttctg
     3181 agaaaaaagg gttatataaa cacttgatga ccggtgtatc acatatgtta ccgctggtgg
     3241 tagcaggtgg cttgctcatc gctatttcct ttatgttcgg aattgaagcc tttaaagatg
     3301 aaaccatcgc aggcggcttg ccgaaagcct taatggatat tggtggcggt gcggcattcc
     3361 acttaatgat tgcggtattc gcaggctatg tagcgttctc aattgcagac cgtccggggt
     3421 tagctgtcgg tcttatcggc ggtatgcttg ctaccaccgc aggagcaggg attttaggtg
     3481 gtatcgtagc cggtttctta gccggttata ccgtaaaatt cttaaatggt gcgattcagc
     3541 taccggcaag tttaacctca cttaaaccta ttttgatttt accactctta ggctcagcga
     3601 ttgtcggctt actgatgatt tatgtcatca acccaccggt gaaagcatta atggatgcct
     3661 tagtggaatg gttaaacaca atggggcaaa ccaatgcaat tattttaggt attattctcg
     3721 gtgcgatgat gtgtaccgat atgggtggtc cggtgaataa agcggcttat acctttggtg
     3781 tgggtttaat tgcttcacaa caatatatgc caatggcagc ggtgatggcg gcaggtatgg
     3841 ttccaccaat tggtatggcg attgcgaccc tgattgctcg tagcaaattc aacaccaacc
     3901 aacgtgatgc aggtaaagct tcattcgtgt taggtttatg ctttatttca gaaggggcat
     3961 taccgttcgt ggcggcagac cctgtgcgtg taattgccag ctcaatctta ggcggtgcaa
     4021 ccgccggtgc aatttcaatg gcactaggca ttaccctaca agccccacac ggcggtttat
     4081 tcgtgattcc attcgtttca caaccgttaa tgtacttagc tgctattgcc atcggctcat
     4141 tagtcaccgg cgtagtctat gccgctatca aacaaaaagc agaataaagc acaaacacaa
     4201 atacaaacaa aaacggagcg aaacgctccg tttttatatc atattggtac ttactgaatt
     4261 ctatttcatc gaaatatcaa gtctaaaata ttatttttgc gatcgagatc gaaaaaatca
     4321 ttttcatcac tttacgaata atatcaactc gttatgattt tgctacgagg agatactatg
     4381 cagtttattg aatttaccga tcctgatagt ggaatttctt atcaatacag tgaatttact
     4441 attgctaatg tcgcatttat cattaatttc tgctcagatg ccgatgttat atcgactttg
     4501 tccgcattag gcaaagatat cacaaactat ataaacacct attcctgttg cacaatcaaa
     4561 tttatggcga aagagcatct agaaaatagc ggttctaata ttgacattta tgcccccgca
     4621 gcgaatcatc agttcaaacg taaagaaata attgctttac aagaaaccct tgagcgttta
     4681 ttatttgagc attatgtacg ttttacacca gaaagctatc tcttcattgc cgagagagat
     4741 tcacttaacc gaatgtatca gcgtatgtgt gtgccacgat gtgattttat gcaatctttc
     4801 caagtggttt atcccttggg cgttaatcaa gattgtttca ttttaattac gccaaaagga
     4861 aatctaaaat gaatcaacaa cgtccagttt ccgttcgttt aaagcaagaa ggcttaatcg
     4921 cttttaagca agcctatcag cgagcaaaaa aagcaggtct attgaaaccg gtcaaataat
     4981 ctttaaaccg cttgctctac aagcgattta tttttcctta attatcacac cttgctacga
     5041 aaactatccc aactcaataa tccaatcgaa accagcaaac agaccatcaa aatataggaa
     5101 ttggcatcga gtttttcgcc gatggcaaaa gaaatcagca acatcaccaa cggttcaaca
     5161 tagcccagta aacctaatac attaatcggt aataaattgc tcgagaggat ataaagaata
     5221 aaagcactgc cacttaccag ccccagcaat ccgaggtaga aataaatttg cggattttct
     5281 gccactattg gcgtcatttc cacttggctg agataataaa gtgcaacggg taataccagt
     5341 aacaactcca caaaaaaagt agctaacgtg ttgatttgaa aataacggcg aagggtaata
     5401 taaagcggat aacccaaccc agctacccaa gtcgcccacg aaatcgtgct gttaaaaaac
     5461 aaactactgc caacaccgat gactgcgaaa agtagagaaa gccatttcag gcgggtaaaa
     5521 tgttctttaa acaccatttt tcctagagcc accgccacaa tcggtaaaag caagtaacca
     5581 aacgacacct gcaacgcctg cccgtgatta ggagcccata aaaacagcca aagctgcacg
     5641 ccggtgttga gagcaaggag caaaatgacc aatacaagcg gtggattttg tcgaattttt
     5701 tgcaaaagat ccgcaaaagc cgattgctgt ttaaacaata taatcgccag caaaataaac
     5761 ggaatgacta ccactatccg aaagccgaac atcgcctctc cctctaacgg gcgaagcaat
     5821 atcgcaaaat agtaaactgc tccaaacagc acattggctg caagcgaagc taaaattcct
     5881 ttgagcattt ggctttcctc ttatcaacaa tcaaacaaca agcggttgac tttgaacaaa
     5941 aatttacaaa tcttatctac gccccatagt atgaaaaaat ttgtaagtaa gctagctata
     6001 ttcgattttt cccgatacaa tgctcccaat ttttcactct tttatttata ttataggact
     6061 tcataatgaa aaagattttt tggattttac tgatcgccat gcttgctgtt ggcggttatt
     6121 ctttaacaaa atacaacgat ttaatgcgtg cagaagaaga catcaactcc gtatggggaa
     6181 atgttgaatc ggcttaccaa cgccgtgcgg atttaattcc aaacttagtg aataccgtaa
     6241 aaggtcaggc taactttgag aaagaaactt taacctctgt gattgaagct cgtgcaaaag
     6301 caactgcggt aaccattgac ccaagcaatg caactgaaga gcaaatggct aaattccaag
     6361 aagcccaaca aggtgtaaac tctgctcttt cacgcttatt ggtttcagtt gagaaatacc
     6421 ctgaattaaa agcccacgaa gcgtttttaa atctacaagc tcaattagaa ggtacggaaa
     6481 accgcattaa cgttgaacgc aataacttta acgaaaaagt gaaagagtac aacaagcaag
     6541 tgcgtgaatt cccgactaaa ttagcggcaa tgattatggg ctttaaagca aaaccgcaat
     6601 ttaaatctga agcaggttct gataaagcac caaccgtaaa cttcaactaa ttttagtttc
     6661 aatttattca tattaaaagc aataagctct ttcaaatccg gtgcttattg ctttattttt
     6721 aaggggaaca aatgggatta ttttcttttc tctccaacaa attaccgata gatacgcaac
     6781 gcattgaaca agcaattgcc gatttagagc aaaaaacctc tgccgaattg cgtgtggtgg
     6841 tggaacgaaa agccaaaatc ggcaaaaccg acaacgccgc tatgttgcga gcaagtgagc
     6901 ttttcgatga attaaaaatg cgtgaaacgg ctgaacataa cggtgtgttg atctaccttt
     6961 cattcaagcc tcactacgct gcgattattg gcgatgaagg cattcatcaa aaagtcgata
     7021 acgatttttg gcaaaaaatc tatgccacaa tgtgtttgga ctgccaacaa ggcgattaca
     7081 cccaagccat ttgtgatgca atcaaaatgg tggaagctcc gctcgctcac catttccctt
     7141 atcaggctga tgataaaaac gagctttcaa acgaggtggt agtcaaatga tgaaatccct
     7201 ttgcaaattt ctatctattt tcaaccgctt gttattggtg tgcttggctc tgtttagcct
     7261 aaatgccaca gcggtgacct acccagagcc gcctaatccg ttttattata ttacggacta
     7321 caccaaaaac accttgagcc aacaagaatg gcggacatta gaagatgcct taattgctaa
     7381 ctgtgccaag acaagttcgc aaattgccgt ggtgattatt ccaagcacgg aaggcgaggc
     7441 agtttcaacc tatgctcata ccctgtttaa caaatgggga atcggacgga gcaaaaataa
     7501 cgagaacaat ggtatattgt tattaattgc caaaaatgat cgcaaactct ttattgccac
     7561 cggacgtggg cttgaaggag cgttacctga tgcgattgca tcgagcatta tccgcaatga
     7621 catcacgcca ttcttcaaac aagagcaata tgcagccggt atcgcccatg gcttatccgc
     7681 cattatggca gccattaacg gggaatatgc cccttatgca agttatggcg aagaaatgca
     7741 agaatttgat gacatagacg gcttactctt tttcttgttt gccggcattg tgattttcct
     7801 aattttccgc ccacgaggaa acagtaccta tattagtccc agctcaatgg atcaactagg
     7861 acaaatacta agagaaagcc aacgcagagg tgggggcttt ggcggtggtc tcggaggtgg
     7921 ctttgatcga ggctcaggtg gatttggcgg aggcttcggt ggtggttttg gcggaagcag
     7981 aggcagtggc gacagcttcg gtggtggttc atcaggcggt ggaggcgctg gaggaagctg
     8041 gtagtttccg cccaatttaa aacaatacaa gcggtcatat ttctcaaaaa aattgcgtat
     8101 ttttcgctaa acgcacaaaa cgctaatgaa aataagaccg cttcctcact tctctaagct
     8161 atgccctttt tagtaacaat gctacggctt cacaagctat gccttcacct cgcccggtaa
     8221 accctaattt ttctgttgta gtggctttta cattcacttg tgatatatcg cattgtagat
     8281 cgtctgcgat cacctgtctc attttatcaa tatgcggtcg catcttcgga gcttgagcaa
     8341 taatggttac atcgacgtta ccgacttgat agccctgttc ttgcacttgt cggtaagctt
     8401 caatcaacaa accacgacta tcggcatttt tgtattgcat atcagtatca gggaataatt
     8461 tgccaatatc acctaaagct gctgcgccaa gcaacgcatc tgttaaagcg tgcagcgcta
     8521 catctccgtc actatgcgca atgaagcctg tatgataagg cacttcaacc ccagcaatca
     8581 tcagtggtct atcttcgcca aaagcgtgaa catcaaaacc gtgtccaata cgtatcattt
     8641 tagttctcca aattatgtgt aaggtaaaat tccgctaagg ctaaatcttc agggcgagtg
     8701 attttgatat tatcacttct acctgaaacc aattgtggct ggtagcctgc aagctccatt
     8761 gcagatgctt cgtctgttac ctgaagctta ttggcaaaag cagaggataa tgcttgtttt
     8821 aatgcttgag caggaaaaaa ttgtggggtt aaagcgtgcc ataaggttga gcgatcttct
     8881 gtgtgtgcga ttttcgtgcc gtttgctctt ttcatcgtat ctaccgctgg aatggcgaga
     8941 atgccacctt gcggatcgtt aatttgcaaa agtttatcta aatctgaccg cttgaggcaa
     9001 gggcgagccg catcgtgaac taatgcccaa gcattttcag tgttgatttc ctgtaaggca
     9061 tttagcactg aatctgctct cgtttctcct ccaaaaacaa gttggatctt aggggatttt
     9121 aggagttcca atgtggaata aaaagggtct tttcttgcca ccgctacaat cactttttca
     9181 acatgagggt gttcaagaaa aatagacact gtatgctcta agatagtctt accctttaat
     9241 tgtaaatatt gttttggttt ttctgcttgc atccggctac caactcctga cgccggaatt
     9301 actgcaataa tttttcgctt cataatatat caattatgta ttatttgttt tttggtacaa
     9361 ttcggtagaa catctcgttc tcttttacca tttctcgttc aagtcttgct ctctcttcaa
     9421 gtgcatctac gccgttcttt aaatcgtaaa tttcggcttc aattagttta ttgcgtgaag
     9481 taagctttgt gttttctact tttaataaag caatttcttc ttgcacttct tgataatcac
     9541 tccaaccatt ttttccaaac caaaagctat attggaaaaa gccgagtaat agggcaaaaa
     9601 aagtaattaa taaa
//
LOCUS       DANLZC010000098         9473 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.098, whole genome shotgun sequence.
ACCESSION   DANLZC010000098 DANLZC010000000
VERSION     DANLZC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.098"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(173..1315)
                     /gene="argE"
                     /locus_tag="PXE44_001817"
     CDS             complement(173..1315)
                     /gene="argE"
                     /locus_tag="PXE44_001817"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDL5589306.1"
                     /translation="MKQIPFIQRYAQLIELPTISSLVAEEDLSNRRLIELLATWLADF
                     GFKTEILAVEGSRNKYNLLATYGEGEGGLLLAGHTDTVPFDEGKWRFNPFQLTEKAGK
                     LYGLGTADMKGFFAFVVEVVSQLDLTQIKKPIRILATADEETTMLGARTFAQHSHIRP
                     DCAIIGEPTSLKPIRAHKGHIGESVRITGRSGHSSDPAKGINAIELMHQSIGYLMKMR
                     DELREKYHNPLFQVTHPTMNFGNIHGGDAINRICACCELQFDMRPLPNMAVQDLYAMV
                     NEHLKPMLEQYGDLIEIRHLHDGIPGYECEHSAQIVQVVEKLLGEKCDAVNYCTEAPF
                     IQQLCPTLVLGPGSIEQAHQPDEFLEMKYIEPTKELLTKLIYHFCG"
     gene            complement(1319..2110)
                     /locus_tag="PXE44_001818"
     CDS             complement(1319..2110)
                     /locus_tag="PXE44_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL5589307.1"
                     /translation="MKTEWIKHLIQQLTTLSKAIENYFTSGSFGVKFYPFLMHPCFRK
                     SCAFLIFIIAMVSSFSGLFYLVAEHITILFSERSLTTYFHHSTLELMIPVGTMIDEKI
                     SELSPLSVVMRSMFVLQAVVFFFIYAIGITHIAHNKLRVIGLLLSLCFAIGCSLVAGI
                     QPSSRGEFGIYNLGASITFLIGNLTLIIAGFDLYHPSMQFFKRFSIGAGVIGAICILI
                     TIFLPTLFSPLIERAGIYTIMLWEILAGFAIIKRLLKLPSFRQQG"
     gene            complement(2126..2731)
                     /locus_tag="PXE44_001819"
     CDS             complement(2126..2731)
                     /locus_tag="PXE44_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HDL5589308.1"
                     /translation="MLDQTIIDQYDALIFDMDGTLIDTMPSHAKAWEKVGEVLGYPIN
                     PKPMYELSGSTTFVIAREIMQRSNIPEHYFEQVVQLKREFGIEMVLANATLLPAFEII
                     KANVGKKPMAIGTGSHRAMVELLDQKFNLRQYVSVIVDSDDVSNHKPAPETFLKCAEK
                     LGIAPHRCLVFEDADLGVQAALNGNMNVFDVRTNTITHSTR"
     gene            complement(2752..3660)
                     /gene="rdgC"
                     /locus_tag="PXE44_001820"
     CDS             complement(2752..3660)
                     /gene="rdgC"
                     /locus_tag="PXE44_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDL5589309.1"
                     /translation="MVWFKNVMIYKLTGELALESNSLNELQQETRFTPCSQHDMSKFG
                     WATPLTGSDLLHFSQANQFLLISHKEDKLLPANVVKAETEQRIAELEEKEARKLKKTE
                     KQAVKDQVVSALLPRAFSKHQFTALWLDLDNQLVYVDAASSKRAEDTLALLRKTLGSL
                     PVVPISFTLPPAEVMTNWIAKGHTPSWLNLLEEAELKSFDTDSIIRCKRQDLETEEIA
                     RHLQAGKFITKLAIEWEGHFNCVLNEDATLSRLKFADEVREKNDDILKEDVAQRFDAD
                     FLLMTEELKLFTEKMIAEFGGIKSRI"
     gene            3737..4555
                     /locus_tag="PXE44_001821"
     CDS             3737..4555
                     /locus_tag="PXE44_001821"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL5589310.1"
                     /translation="MNEKKLAFIGTGNMAYAIIQGLLKSGYSAEQIIACNKSNLARRE
                     ELQAVGIATNFANREAVEQADVIVLAVKPQMMAQVCAEFADVDFSNKWVISVAAGISV
                     QRLETLLPSAKNIIRTMPNTPSLISEGMTGLFAKKSVNPTACQFAEQLMSAVGKCYWV
                     ETEEQINHIIAITGSSPAYFFRFMEAMQQSAVQMGFSEQDARTLVQSVALGAAKLVEA
                     NPTTPLSTLRENVTSKGGTTAKALEVFEQHDLAKTVDDAMWAAIHRAREMEQSL"
     gene            4552..5706
                     /locus_tag="PXE44_001822"
     CDS             4552..5706
                     /locus_tag="PXE44_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDL5589311.1"
                     /translation="MMKVSPFQWAAFNYFGFFCAYGVLLPFLPVWLKHHGYSTEMIGL
                     FAAVGYLFRFAGSMLASQRVKTVSQLIPTARSLTWLNIAAAAALLFSGDFIWLLFPVL
                     MLFQMFNSGAMPIADSIASIWQHQIGLDYGRARLFGSMAFVVGSLSAGYLIGLLGENS
                     VVWIIIGFLVFLGIGQLATPKVGFEEQGNKANTSSVTYRQLLKEPETLKMLIAVSLIS
                     ASHAAYYTYSTIHWSNASISTAMTSLFWGLAVVAEILFFLVSKRLFKSWKISHLMILA
                     AVFAVIRWAIMGSTTEIVLIIVMQMLHSITFGAAHIAMIRYISMQSAERVTKLQGLYF
                     GLASCAFMAVFTFIAGMVYQHSPSLTFWLMAIFTLPAIFIVPKRFEVQIN"
     gene            complement(5766..5978)
                     /locus_tag="PXE44_001823"
     CDS             complement(5766..5978)
                     /locus_tag="PXE44_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL5589312.1"
                     /translation="MSAITGTVKWFNATKGFGFITPDQGGKDVFVHFSAIEANGGYRT
                     LEDGAKVQFEIQDSERGASAVNVKTI"
     gene            6306..6842
                     /gene="proQ"
                     /locus_tag="PXE44_001824"
     CDS             6306..6842
                     /gene="proQ"
                     /locus_tag="PXE44_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDL5589313.1"
                     /translation="MSEQIEQKVEQTQNKTNPSTKEVIAYLAEKFPLCFTLEGEVKPL
                     KVGLFQELAEALKDDEKISKTLLRQVLRNYTNSWRYLAASQANVARVGLQGEEVGVVT
                     EDEATHAAEALAAAKAVVAERKAQERKAQRKEYFKQKAREENAKKRAEFKAKKAIDNL
                     PKASEEKLAALADKFGRK"
     gene            6928..8940
                     /gene="prc"
                     /locus_tag="PXE44_001825"
     CDS             6928..8940
                     /gene="prc"
                     /locus_tag="PXE44_001825"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDL5589314.1"
                     /translation="MKLTRLNKIFALALGLMIGNAFAVQPDIKESAIVMPKPNEQHSL
                     STKRVTARLTQSHYHKFQLNDEFAGKIFNRYVDWLDPSHNTFLQADIDEMRSKYATTL
                     DDDLYAGKLDAAFAMYELMSKRRYERYSYALSLLDKEPDLKGNDEIEIDREKAAFPKS
                     VEEANTLWEQRVKNDIINLYLKDKKWPEIKKTLTKRYNFAIKRLTQVKADDILQTYLN
                     SFSREIDPHTSYLSPRAAKAFQESMNLSLEGIGATLTQEDDVTSIRALVPGAPAAKSK
                     KLVVGDKIVGVGQGERGEIEDVIGWRLDNVVDKIKGKKGTKVRLEIEPEKGGKTKIIT
                     LVRDKVRIEDSAAKLTVEKVDGKNVGVIKIPTFYIGLTSDVRKLLTQIEKKNVEGLII
                     DLRENGGGSLTEVIELTGLFIKDGPVVQVQDAFNRIKVHEDPESKSVYDGKVMVMINR
                     HSASASEIFAAAMQDYNRAIIVGQQTFGKGTVQQSRSLNFVYDLDQEPLGFIQYTIQK
                     FYRINGGSTQLKGVEADIKFPEIINAAKTGESFEDNALPWDKIPAANYKEAGNARNAV
                     AALTAKHQERIAKNPEFIVLNENIAIRKERDERKFLSLNLEERLKENKADESKRLKDI
                     NARFAREGKKALKDIDDLPKDYEAPDFFLKEAEQMMLDWSEFDKKS"
     gene            9031..>9473
                     /locus_tag="PXE44_001826"
     CDS             9031..>9473
                     /locus_tag="PXE44_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDL5589315.1"
                     /translation="MKTFKLLPIMVAFSGMALAETATVAQVASEPVVQDNILPQLSFA
                     AQQARAAELVKQADYALEQEQIRQEQARKFAEAEARIKQEIGNNALLLSQATTKVYLD
                     SEFATMWNDKAAEKAFLKEYALFAVSGVSSKSAKALQQILNQPEG"
BASE COUNT         2781 a         1821 c         2129 g         2742 t
ORIGIN      
        1 gccgtttgta tccaagacct agctagagat taatacctat ctaacagaat ctccttaaat
       61 caatgatgaa gtatggaagt ttataggtca ctcaatacta aatatcaagc ggtcattttt
      121 gccgattctt ttgcaaaaag tatggcgaaa atgagcgctt gtttctgttg atttatccac
      181 aaaaatgata aatcaattta gtcaataact cttttgtcgg ttcgatatat ttcatttcca
      241 aaaactcatc gggctggtgg gcttgttcaa tggagccggg gcctagtacc agcgttgggc
      301 agagttgttg aatgaatggg gcttcggtgc agtaattgac ggcatcgcat ttttcgccca
      361 gtagcttttc aaccacttgc acgatttgtg ccgagtgttc gcattcgtag cccggaattc
      421 catcgtgtaa atggcggatt tcaattaaat cgccatattg ttccagcatc ggttttaaat
      481 gctcgtttac cattgcatac aaatcttgca ccgccatatt cggtagtggt cgcatatcaa
      541 attgcaattc gcaacaggca caaatgcggt taatggcatc gccgccgtga atattgccga
      601 agttcattgt cgggtgggtg acttggaaca gcggattgtg gtatttctct cgcagttcat
      661 cacgcatttt cattaaataa ccgatagatt ggtgcattaa ttcaatcgca ttaatgcctt
      721 tggcggggtc gctggagtgt ccgcttctgc cggtaatgcg aaccgattcg ccaatatgcc
      781 ctttgtgggc tcgaatcggt ttgagcgagg tcggctcgcc aataatcgca caatcggggc
      841 gaatgtggct gtgctgagca aaggttcttg cccccagcat tgtggtttct tcatcagcgg
      901 tggcaagaat gcgaatcggc tttttgattt gggttaggtc gagttggctc acaacctcca
      961 ccacaaaggc gaaaaagcct ttcatatctg ccgtgcctaa gccgtagagt ttgcctgctt
     1021 tttcggttaa ttggaatgga ttgaagcgcc attttccttc atcaaacggc acggtgtcgg
     1081 tatgccctgc caataataag ccgccctcac cctcgccata ggtcgcaagt aaattgtatt
     1141 tattacggct gccttctacc gctaaaatct cggttttaaa gccgaaatct gccagccaag
     1201 tggcgagcaa ttcaatcaaa cggcggttcg ataaatcttc ttcggcaaca aggctggaaa
     1261 tggtcggtag ctcaatcagt tgagcatagc gttgaataaa gggaatctgt ttcattggtt
     1321 atccttgttg tctgaatgag ggcaatttca acaggcgttt aatgatggca aaaccggcta
     1381 aaatttccca cagcataatg gtataaatac ctgctcgttc aattaacggg ctaaacaggg
     1441 ttggtaggaa aatcgtgatt aaaatacaga ttgccccgat aacgcccgca ccaatgctaa
     1501 agcgtttaaa aaattgcatt gaagggtggt agagatcgaa gcctgcgatg atcagcgtta
     1561 aattgccgat taaaaacgta atgcttgccc ctaaattgta aatgccgaat tcgccacgac
     1621 tgcttggctg aataccggca actagtgagc agccaatcgc aaagcatagc gaaagcaata
     1681 agccgatgac ccgcaatttg ttgtgagcaa tgtgggtgat gccgattgca taaataaaga
     1741 aaaaaacgac tgcttgtagc acaaacattg aacgcattac caccgaaagt ggtgataatt
     1801 cgctgatttt ttcatctatc attgtgccga ctggaatcat caattcaagg gtgctatggt
     1861 ggaagtaagt ggtgagcgat ctttcgctaa ataaaatcgt gatgtgttcc gccaccaaat
     1921 aaaatagccc gctaaaactt gaaaccatag caataataaa aatcaaaaag gcacagcttt
     1981 tacgaaaaca agggtgcatt agaaaagggt agaatttcac cccaaatgaa ccgctggtaa
     2041 agtagttttc gatggctttg gaaagcgtgg ttaattgttg aattaaatgt ttaatccatt
     2101 ctgttttcat ataaacctat catttttaac gggttgagtg agttatcgta ttcgtacgta
     2161 catcaaacac gttcatattg ccgttgaggg cggcttgcac gcctaaatcg gcatcttcaa
     2221 acaccaaaca gcggtgtggg gcaatgccaa gtttttccgc acattttaaa aaggtttcag
     2281 gggctggctt atggttgctt acatcatcag aatctacaat aaccgaaaca tattgacgta
     2341 agttaaattt ttggtctaat aattccacca ttgcacggtg tgagcctgtg ccgattgcca
     2401 ttggtttttt gccaacgtta gctttaataa tctcaaaggc aggcaacaga gtggcattgg
     2461 ctaacaccat ttcaatccca aattcccgtt ttagttgtac cacttgctca aaataatgct
     2521 cggggatatt ggagcgttgc attatttctc gggcaatcac aaaagtggtt gagccactca
     2581 gttcgtacat tggctttgga ttgattgggt agcctaacac ttcgcccacc ttttcccacg
     2641 ccttggcgtg gcttggcatc gtgtcaatta atgtgccgtc catatcaaaa atcaaggcgt
     2701 catattggtc gataatggtt tgatctaaca ttgctttcct taaatttttt tctaaattct
     2761 ggatttaatt ccgccaaatt cggcaatcat tttttcggtg aaaagtttca gctcttcggt
     2821 cattaataag aaatcggcat caaagcgttg agccacatct tctttgagaa tatcgtcatt
     2881 tttctctcgc acttcatcgg caaacttcag gcgtgaaagc gtggcgtctt catttaacac
     2941 gcaattgaaa tgcccttccc attcaatcgc aagttttgtg ataaatttgc ccgcttgcaa
     3001 gtgccgggcg atttcttccg tctctaaatc ttggcgttta cagcgaataa tgctgtcggt
     3061 atcgaaagat ttcagttctg cctcttccaa caaattcaac caacttggcg tatgcccttt
     3121 ggcgatccaa ttcgtcatta cctctgccgg cggtagggta aaggaaatcg gcacaaccgg
     3181 caacgagcca agtgtttttc gcagtaacgc cagcgtatct tccgcccgtt tgctcgaggc
     3241 tgcatccaca taaaccaact gattatctaa atccaaccaa agagcggtga attgatgttt
     3301 gctgaatgct cgaggcaata acgcagaaac gacctgatct ttcacggctt gtttttcggt
     3361 ttttttcagc ttacgagcct ctttttcttc cagttctgca atgcgttgct cggtttcggc
     3421 tttcaccaca ttggcaggta ataatttatc ttctttgtgt gaaatcagca aaaattggtt
     3481 cgcttgtgag aaatgcagta aatccgagcc ggtaagtggc gtagcccagc cgaatttgct
     3541 catatcgtgc tggctgcaag gggtaaagcg ggtttcttgc tgtaactcgt taaggctatt
     3601 gctttccaac gccagctcgc cggttaattt atagatcatc acatttttga accaaaccat
     3661 ttttgcatat cctttatcgg gtgtagaata gtgttatttt aacagacctc aacccaacac
     3721 agtagagaat ttgcgaatga acgagaaaaa acttgccttt atcggcaccg gaaatatggc
     3781 gtatgcgatt attcaaggct tattaaaaag cggttattct gccgaacaaa tcattgcgtg
     3841 caataaaagt aatttggctc gccgagagga gttacaagcg gtcggaattg cgacaaattt
     3901 tgcaaatcgt gaggcggtag agcaggctga cgtgatcgtg ctggcggtaa aaccacagat
     3961 gatggcacaa gtgtgtgccg agtttgccga tgttgatttt tccaacaaat gggtgatttc
     4021 agtagcagca ggtatttctg tgcaacgctt ggaaacgctg ttgccgagtg cgaaaaatat
     4081 cattcgcacg atgccaaaca ccccgtcttt aatcagcgaa ggaatgaccg gattatttgc
     4141 aaaaaaatcg gtaaatccga ccgcttgcca atttgccgaa cagttgatgt cagccgttgg
     4201 taaatgttac tgggtggaaa ccgaggagca aatcaaccac attattgcga taaccggctc
     4261 aagcccggcg tatttcttcc gttttatgga ggcaatgcag caaagtgcgg tgcaaatggg
     4321 cttttccgag caggacgcaa gaacgcttgt acaatctgtc gcattggggg cggcgaaatt
     4381 ggtggaagcg aacccgacta cgccactttc gaccttgcgg gaaaatgtga cctcaaaggg
     4441 aggcacaacg gcgaaagccc ttgaggtgtt tgagcagcac gatcttgcta aaaccgttga
     4501 tgatgcaatg tgggcggcaa ttcatcgagc cagagaaatg gagcaatcgt tatgatgaaa
     4561 gtttccccgt tccaatgggc ggcatttaat tactttggct ttttttgtgc ctatggcgtg
     4621 ttgttgccgt ttttgccagt gtggcttaaa catcacggct attcgactga aatgatcggc
     4681 ttatttgccg ccgttggcta tttgttccgc tttgccggca gtatgcttgc ctcacaacgg
     4741 gtaaaaaccg tgagccaact cattcccacc gcccgcagtt taacttggct gaatattgcg
     4801 gctgcggcgg cgttgctgtt ttcaggggat ttcatttggc tactttttcc ggtgttaatg
     4861 ttgtttcaaa tgttcaattc gggggcaatg ccgatagccg atagcattgc ctcaatttgg
     4921 caacatcaga tcgggctgga ttacggcaga gcaaggttat tcggctcaat ggcgtttgtg
     4981 gtaggctcac tttctgccgg ctatcttatc ggcttgcttg gtgaaaattc ggtggtgtgg
     5041 attatcatcg gtttcttggt ttttttagga atagggcagc ttgctacgcc caaagtcggg
     5101 tttgaagaac aaggtaataa agccaatact tcaagcgtaa cttatcgcca attattgaaa
     5161 gagccggaaa ccttaaaaat gttgatcgcg gtgtcgttaa tttcagcctc gcacgccgct
     5221 tattacactt acagcaccat tcattggtca aatgccagta tttcaaccgc aatgactagc
     5281 ctgttttggg gcttggcggt ggtggcggaa attttgttct ttttggtctc taaacgcttg
     5341 tttaaatcgt ggaaaatcag ccatttaatg attttagctg ccgtttttgc agtgattcgt
     5401 tgggcaataa tgggtagtac tacagagatc gtgctgatta ttgtaatgca aatgctacat
     5461 tctattacct tcggggcggc tcatattgcg atgattcgct atatttcgat gcaatcggca
     5521 gagcgtgtga cgaaattaca aggtctctat tttggcttgg caagctgtgc ttttatggca
     5581 gtatttacct ttattgccgg aatggtctat caacattccc caagcctgac attttggttg
     5641 atggcaattt ttaccctacc tgccattttt attgtgccga aaaggtttga agtacagatt
     5701 aattgatagc aaaaatgccg acaagttgtc ggcattttta tcgaaattca ttgaatttgc
     5761 tgaaattaga ttgttttcac gtttacggct gaagctccac gctctgaatc ttggatttca
     5821 aattgtactt tagcaccgtc ttctaatgtg cggtagccgc cgtttgcttc gatagcagag
     5881 aagtgtacga atacatcttt gccaccttga tcaggggtga taaaaccaaa acctttggtg
     5941 gcgttgaacc attttactgt gccggtaatt gctgacataa aattttcctt atataaatac
     6001 aaaaagtgat aaaagatagc taaacgggat attacagacg tatctatcaa tcacttgcct
     6061 tgaggaaaat aactaacaag attcaacttt aattaacgca cgaacagctt gagccattac
     6121 accacttatt cattttttaa gcaaatcatt tttatacttt tatgaacatt caaatgaaaa
     6181 aagagtctat ctcttttgta aacgtaatgt ggaatttata tggacgaagc cttataaatc
     6241 aaatatagta tccagttaga ttttcactta tctaattatt agatttttat taaatgggac
     6301 ttattatgtc agagcaaatt gagcaaaaag tagaacaaac tcagaataaa accaatccaa
     6361 gtaccaagga agtgattgct tatttagcgg agaaatttcc gctttgtttc acgcttgaag
     6421 gtgaagtaaa accattgaaa gttgggttgt ttcaggaatt agctgaagca ttaaaagatg
     6481 atgagaaaat cagtaaaacg ttattgcgcc aagtattgcg gaattacact aattcttggc
     6541 gttacttggc tgccagccaa gctaatgttg ctcgtgtcgg cttacagggt gaagaagttg
     6601 gcgtggtaac agaagacgaa gctactcacg ccgcagaagc tttggctgct gcgaaagcgg
     6661 tggttgcaga acgtaaagca caagagcgta aagcacaacg taaagagtac ttcaaacaga
     6721 aagcacgtga agaaaatgcg aaaaagcgtg cagaatttaa ggcgaaaaaa gcaatagata
     6781 atttacctaa agcttcagaa gaaaaattag ctgcattagc agataaattc ggtcgtaaat
     6841 agttttcaag tcgctttgtg cttacaagcg gtcggatttt agtaaaattt tgcaaaaaaa
     6901 tgaccgcttg attatttgga aaagaagatg aaattaacaa gattaaataa aatttttgcc
     6961 ctagccctag gtttaatgat aggaaacgct tttgccgttc agccggacat taaagaaagt
     7021 gcgattgtga tgccaaaacc taatgagcag catagcttat caaccaagcg tgtaacagcc
     7081 cgattaactc aatcgcatta tcataaattt caactgaatg atgagtttgc agggaagatt
     7141 tttaaccgct atgtcgattg gcttgatcct tctcataata cttttcttca agccgatatt
     7201 gatgaaatgc gtagtaaata tgctacaaca ttagatgatg atttatatgc tggtaaatta
     7261 gatgcagcat ttgcaatgta tgagttaatg tctaaacgtc gttatgagcg ttatagctat
     7321 gccttatctt tactagataa agagcctgac ttaaaaggga atgatgaaat tgagattgac
     7381 cgtgagaagg ccgcatttcc aaaaagtgta gaagaggcaa atacactttg ggagcagcga
     7441 gttaaaaacg atattatcaa tttatatctt aaagataaaa aatggcctga aattaagaaa
     7501 acactcacta aacgttataa ctttgcgatt aagcgtttaa ctcaagttaa ggctgatgat
     7561 attttacaaa cttacttaaa ttcattctct cgtgagatcg atccccacac tagctatttg
     7621 tctcctcgtg cggcaaaagc gttccaagag agcatgaatc tgtcgctaga aggaattggt
     7681 gcaacgctaa cccaagaaga tgatgtaacc agcattagag cattagtgcc aggagcccct
     7741 gccgctaaaa gtaagaagct ggtagtcggc gataaaattg tcggtgtagg tcaaggtgag
     7801 agaggtgaga ttgaagatgt tatcggttgg cgtttggata atgtagtcga taaaatcaaa
     7861 ggtaaaaaag gcactaaagt tcgtttagaa attgagcctg aaaaaggtgg aaaaactaaa
     7921 attattaccc ttgtgcgtga taaagttcgt attgaggata gtgctgctaa attaacggta
     7981 gaaaaagtag atggaaaaaa tgtaggggta attaagatcc ctacttttta tatcggtttg
     8041 acttcagatg tacgtaaatt acttactcaa atagagaaaa aaaatgttga aggattgatc
     8101 attgatttac gtgaaaacgg tggtggttcg ttaactgaag tgattgaatt aactggttta
     8161 tttattaaag atgggcctgt agtgcaagta caagacgcat tcaatcgtat caaagtacac
     8221 gaagatccag aatcaaaatc agtttatgat ggaaaagtga tggtgatgat taatcgccat
     8281 agtgcttcag cctctgaaat cttcgctgcg gcaatgcaag attacaaccg tgcaattatt
     8341 gtagggcaac aaacctttgg taagggaact gttcaacaga gccgttcatt gaattttgta
     8401 tatgatttgg atcaagagcc tcttggtttt atccaatata cgattcaaaa attctaccgt
     8461 atcaatggtg gaagtaccca gctaaaaggg gttgaggcgg atattaaatt cccggaaatc
     8521 attaatgctg caaaaacagg tgaaagtttt gaagataatg cgcttccttg ggataaaatt
     8581 ccagcagcta attataaaga ggctgggaat gctcgtaacg cggttgctgc attaacagcg
     8641 aaacatcaag agcgtattgc taaaaaccca gagtttatag tattaaacga gaatattgcg
     8701 attcgaaaag aacgtgatga gcgtaagttt ttatcgttaa atttagaaga acgcctgaaa
     8761 gaaaataaag cagatgaaag caagcgctta aaagatatta atgctcgttt tgcccgtgaa
     8821 ggcaaaaaag cattaaaaga tattgatgat ttaccaaaag actatgaagc accggacttt
     8881 ttcttaaaag aagctgaaca gatgatgctt gattggtcag aatttgataa aaaatcataa
     8941 atgtgtttat aatgcgcaca taacgtaccg cttagctccg ctaagcggtt tattttttta
     9001 taaatttgca aatattaaga ggaagagtgg gtgaaaacct ttaaattatt acctattatg
     9061 gtggcgtttt ctggcatggc tttggctgaa acagccacag ttgctcaggt ggcttctgag
     9121 cctgttgtac aagataatat tttaccacaa ttaagttttg cagctcagca ggctcgtgct
     9181 gcagaattgg taaaacaggc cgattatgcg ttagaacagg aacaaattcg tcaagagcaa
     9241 gcaagaaaat ttgcagaagc ggaagcaaga attaagcaag aaataggtaa taatgcctta
     9301 ttgctgagcc aagcaacaac taaggtttat ttagacagtg aatttgcgac aatgtggaat
     9361 gataaagccg cagaaaaagc atttttgaaa gaatatgcat tatttgcagt aagtggggta
     9421 tcctctaaat cagctaaggc attgcagcaa atcttaaatc aaccggaagg ttt
//
LOCUS       DANLZC010000099         9301 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.099, whole genome shotgun sequence.
ACCESSION   DANLZC010000099 DANLZC010000000
VERSION     DANLZC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.099"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..334)
                     /locus_tag="PXE44_001827"
     CDS             complement(<1..334)
                     /locus_tag="PXE44_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589316.1"
                     /translation="MITSNLERSFHGLNIAQHNYKIFLDESGGNIGKLKHTPNDKKGS
                     NFNRTISSHIGVPPVFVLGGIAQISNANLIAPDWRKLLHLQNKELKFSKIGKNFFNKL
                     GSKELRKVL"
     gene            complement(361..1035)
                     /gene="cas4"
                     /locus_tag="PXE44_001828"
     CDS             complement(361..1035)
                     /gene="cas4"
                     /locus_tag="PXE44_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDL5589317.1"
                     /translation="MGDLQNSAEILPLVQPKFGDDKRSISLSALQHYAFCPRQCALIH
                     NEQVWAENYLTAQGQALHERVDSGEPEVRKGIRFERTVHVAAEKLGISGILDLVERDL
                     KTGELKPVEYKRGKPKLDPIDEIQLCAQALCLEEMTEQTINEGALWYMQTRHRLPIIF
                     STELRTKTLATIAEVRSLLDSGTTPPPEYSKRCKACSLIDLCQPKLLERDRSKGYVVG
                     VFGSLL"
     gene            complement(1035..1898)
                     /gene="cas7c"
                     /locus_tag="PXE44_001829"
     CDS             complement(1035..1898)
                     /gene="cas7c"
                     /locus_tag="PXE44_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HDL5589318.1"
                     /translation="MSIQNRYEFVFFFDVTNGNPNGDPDAGNMPRLDPETSKGLVTDV
                     CLKRKIRNFIEMSYENEAGYEIYVKEKSVLNLQNKRAYEALGIESEAKKLPKEEAKAR
                     EITAWMCKNFFDIRTFGAVMTTEVNSGQVRGPVQLAFAQSIDPIIPLEVSITRMAVTN
                     EKDLEKERTMGRKYIVPYALYRVHGFISAKLAEKTGFSDEDVQKLWQALQLMFEHDRS
                     AARGEMAARKLIVFKHDSELGNQPAHKLFDSVKVERINGEKDTPAKDYDDYCISVQTE
                     GLTGVTVEELL"
     gene            complement(1908..3689)
                     /gene="cas8c"
                     /locus_tag="PXE44_001830"
     CDS             complement(1908..3689)
                     /gene="cas8c"
                     /locus_tag="PXE44_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HDL5589319.1"
                     /translation="MITALTRYYYRLAAQEDRLTGEAKVPSYGFSEEKIGWVLELDSK
                     GNLVNVISNLTEDKKPKPKLMSVPRPEKRTSGIKPNFLWDKTAYVLGIESNPDKATAK
                     DQPINFAEKTFEAFKQYHLDLLADSNDEGLMAIRNFLQQWQPTHFAESICPREILDAN
                     VVFKLVGTSGYIHQREAAQTLWTSLLINQSDNAEKGLCLISGKLSPIQRTHPPIKGVF
                     GGQSSGGSIISFNKESFTSFGKEQGSNAPVSEQAAFAYTTALNYLLRTEKHRLTIGDA
                     STVFWAEADNTTQAEAAEGFFAALMTPPDDEQENQKIFEELQKISKGRPLAEISPELS
                     ENTRFYVLGLAPNAARISVRFWLDTTFGQLAKNLSEHWQDLALEPCPWKTPPSIWRLL
                     LQTASLGKSENISPVLAGEMARAVISGHLYPMSLLSQLVTRIRADGDINGLRVALMKA
                     VLQRKFRKGFIKEGVPMSLNKESDNQAYLLGRLFAVLERIQSQALGELNAGIADRYYG
                     SASAVPFSVFPRLLSGAKHHLSRLRKDKGGMAVNLDKDLGEIIASLPENFPRHLSIDE
                     QGRFAIGYYHQKQSYFAKKETTESIEN"
     gene            complement(3711..4388)
                     /gene="cas5c"
                     /locus_tag="PXE44_001831"
     CDS             complement(3711..4388)
                     /gene="cas5c"
                     /locus_tag="PXE44_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HDL5589320.1"
                     /translation="MPNKIRLHIWGDYACFTRPEMKVERVSYDVITPSAARGILAAVH
                     WKPAIRWVIDKIYVLQPIQFESVRRNELGSKISASKISGAMKRKTVADLYTVIEDDRQ
                     QRAATVLKNVGYVIEAHAVLTKMAGEAETVTKHIEMFKRRAKKGQCFQQPCMGVREFA
                     ANFALIDDDEPLPECFLAENERDRDLGWMLHDIDFEHGNQPRFFRAEMKNGVIDVPPF
                     YAEEVKS"
     gene            complement(4630..6381)
                     /locus_tag="PXE44_001832"
                     /pseudo
     CDS             complement(4630..6381)
                     /locus_tag="PXE44_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250669.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(6371..6832)
                     /locus_tag="PXE44_001833"
     CDS             complement(6371..6832)
                     /locus_tag="PXE44_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589321.1"
                     /translation="MFKPHGSLDWFMAGDVPMTSCIGKVEEPLIITPGVGKYKKGYGQ
                     PFDAHREKANEAIDSASSILCIGYGFNDDHLQTHLTGKIKSGVKTLLLTRGLSENARN
                     VVKEAPNCKALIFDGDKAGTRVVSKQEETFIPNVHWWDVEFFVKEILENGK"
     gene            complement(6991..8127)
                     /locus_tag="PXE44_001834"
     CDS             complement(6991..8127)
                     /locus_tag="PXE44_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5589322.1"
                     /translation="MLLRKAFKFEIRPNGEQVRKMKQFCGCSRFVFNKALAWQNEQYE
                     QDNHHKFSYTKLANLLPQWKKELVWLKDCHSQVLQQSLKDLESAFKNFFQKRADLPKF
                     KKKGVKESFRFPQGCKIEQKNDRLYLPKIGWVRYRNSREIVGEVKNVTVSMKCGRFFV
                     SIQTEFKQEIPTHKGGEIGIDMGIARFATLSNGEFFEPLNAFKTHKGKLAKLQKQLKN
                     KVKFSKNWQKLKDKIGKLHHKIANCRKDFLHKISTQISKNHAMIYVEDLQVANMSKSA
                     KGTAEVHGKNVKQKSGLNRVILDQSWFEFRRQLDYKTQWLGGFLLAVPPQNTSRTCPC
                     CNYTAKENRQTQENFECVECGYTENADVVGAMNVLTRGQAIVQA"
     gene            8174..8590
                     /gene="tnpA"
                     /locus_tag="PXE44_001835"
     CDS             8174..8590
                     /gene="tnpA"
                     /locus_tag="PXE44_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005720092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL5589323.1"
                     /translation="MKKETEIRRGRHVVFNLHVHLVFVTKYRREVFTQAILNDLHSIF
                     ATICADFEATLVEFDGEDDHVHLLVEYPPKVAISHLVNSLKGVSSRMIRKKNYPSIRK
                     KLWGNQLWSPSYFAGSCGGAPISIIRQYIEQQQTPD"
     gene            complement(8652..9245)
                     /locus_tag="PXE44_001836"
     CDS             complement(8652..9245)
                     /locus_tag="PXE44_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589324.1"
                     /translation="MREPLLDEDELKKWLQTIFTDNLVIIVGSGLSCAEGLPGMGALA
                     DRLKERMPECLDDIDKVTWNTISDCLDSEGLEGALLKHQANETIEAAIIKITAEYVLS
                     EEQKAINKCIAENQKLKFSYLLPHISASNPKIARVITTNYDRLIEFAAEYENWGIDSM
                     MVGRYWGKHNPDLSRKLQIRDIRVKGKCPKLVYLIVP"
BASE COUNT         2560 a         2094 c         1722 g         2925 t
ORIGIN      
        1 ccaagacttt tcttaattct tttgaaccaa gtttattaaa aaaattcttt cctattttac
       61 taaactttaa ttccttattc tgcaaatgaa ggagttttct ccaatcagga gcaattaaat
      121 tagcattact aatttgagca atccctccta atacaaatac aggaggaacc ccaatgtggg
      181 aagaaatggt tctattaaaa ttactacctt ttttatcatt aggcgtatgt tttaacttcc
      241 caatatttcc accactctca tcaagaaata ttttgtagtt atgctgtgct atatttaatc
      301 cgtgaaatga tctttctaag tttgaagtta tcacaacaag tccctcccca aagtaggtaa
      361 ttataataaa gatccaaaca ctcccaccac ataccccttc gatctatccc tttccaacaa
      421 ctttggttgg cagaggtcga taagcgagca ggctttgcag cgtttgctgt attctggagg
      481 aggtgtggtt ccgctatcta agagcgaccg cacttcggca atggtggcga gggttttggt
      541 gcgtagttcg gtggaaaaaa tgatcggcaa acggtggcga gtttgcatat accacaacgc
      601 cccttcgtta atcgtctgct ctgtcatttc ttccaaacat aaggcttggg cacagagttg
      661 gatttcatca atcggatcaa gtttgggctt gccacgtttg tattccactg gttttagctc
      721 gcctgttttt aggtctcgct caaccaaatc caaaatgccg cttatgccta atttttcggc
      781 tgcaacgtga accgttcgct caaagcgaat ccctttgcga acttctggct caccactatc
      841 tacccgctcg tgcaacgctt gaccttgtgc ggtgagatag ttttctgccc acacctgttc
      901 gttgtggatc agcgcacatt gacgtgggca gaaggcatag tgttgcaaag cggaaagcga
      961 aattgaccgc ttgtcatcgc caaattttgg ctgaacaagc ggtaaaattt ccgcagaatt
     1021 ttgcaaatcg cccattacag taattcttca acggttacac ccgttaagcc ttcggtttgt
     1081 acgctgatac agtaatcgtc atagtctttc gctggcgtgt ctttttcacc gttgattcgc
     1141 tcaactttta ccgagtcaaa cagtttgtgt gcaggttggt tgcctaattc gctgtcgtgt
     1201 ttaaatacga tcagtttacg agctgccatt tcgcctcgtg cggcagaacg gtcgtgttca
     1261 aacataagtt gcaaggcttg ccaaagtttt tgtacatctt catcagaaaa gcccgttttt
     1321 tcagcgagtt tagctgaaat aaagccgtga acacgataaa gtgcgtaagg cacgatatat
     1381 ttacgcccca tagtccgttc tttttcaaga tctttttcgt tggtcactgc cattcgtgtg
     1441 atcgatactt ctagcggaat aatcggatcg atagattgag cgaacgccaa ttgcactggg
     1501 cctcgcacct gtccgctgtt tacttcggta gtcattaccg ccccaaaggt gcgaatatcg
     1561 aaaaagtttt tgcacatcca agcggtaatt tctcgggctt tagcttcctc ttttggtagt
     1621 tttttcgctt ccgattcaat ccctaatgct tcataagcac gtttgttttg taaattcaga
     1681 acactttttt ctttaacata aatttcgtaa cccgcctcgt tttcatagct catttcaata
     1741 aaattgcgga ttttgcgttt taggcaaaca tcggtgacta aacctttact ggtttcagga
     1801 tcaaggcgtg gcatatttcc tgcatctgga tcaccatttg ggttaccgtt ggttacatca
     1861 aagaaaaaaa caaattcata gcggttttga atagacatag tgtttcctta gttttcaata
     1921 gactcagtgg tttctttttt agcgaaatag ctttgttttt ggtggtaata accaatcgca
     1981 aaacgccctt gttcatcgat acttaaatgg cgagggaaat tttccggcaa gctggcaata
     2041 atctcaccta aatccttatc cagattaacc gccattccgc ccttatcttt tctcaagcga
     2101 gataaatggt gttttgcacc gcttaataag cgagggaata ccgaaaacgg cacggcagaa
     2161 gccgaaccat aatagcggtc ggcaatgccg gcatttaatt cgcctaatgc ttggctttgg
     2221 attctttcta atactgcaaa taacctgccg agcaagtaag cttggttatc gctttcttta
     2281 tttaagctca taggaactcc ttctttaata aaaccttttc taaattttcg ttgtaagact
     2341 gctttcatca acgctactcg caaaccgtta atatcgccgt ctgctcggat tcgagtcact
     2401 aactgtgaaa gtaaactcat cggatataaa tgaccgctaa taactgcgcg agccatttca
     2461 cccgccaaga ctggagaaat attctcactt ttacctaatg aagcggtttg taataatagt
     2521 cgccaaatgg aaggtggtgt tttccatggg caaggctcaa gagcgagatc ttgccaatgc
     2581 tcgctgaggt tttttgccag ttgcccaaag gtggtatcca gccaaaaacg taccgaaatg
     2641 cgagctgcat ttggagcaag ccccaacaca taaaaacggg tgttttcaga taattctggg
     2701 gaaatttctg caagcggtcg tcctttgctg attttttgca attcttcaaa gattttttgg
     2761 ttttcttgct catcatctgg aggcgtcatt aaagcagcaa aaaagccttc agcagcttca
     2821 gcttgggtgg tattgtctgc ttctgcccaa aagacagtgc tggcatcacc aatagttaaa
     2881 cgatgtttct cggtgcgtaa caaataattt aaagcagtag tgtaggcaaa ggcggcttgt
     2941 tcagaaacag gggcattact accttgttcc ttgccaaatg aagtgaagga ttctttattg
     3001 aaagagatga ttgaaccacc ggaactttgc ccaccaaata cccctttaat aggaggatga
     3061 gttcgttgaa ttggcgataa tttgcctgaa attaagcata aacctttttc agcattatca
     3121 ctttgattta tcaacaaact tgtccaaagg gtttgtgcag cttctcgctg atgaatataa
     3181 ccgcttgtgc cgactaattt aaacacgaca ttcgcatcaa gaatttctct tgggcagatt
     3241 gattcggcaa aatgtgtggg ttgccattgt tgcaggaaat ttcttatcgc cattaaacct
     3301 tcatcattgg aatctgcaag caaatccaaa tggtactgtt taaatgcctc aaaggttttc
     3361 tcggcgaaat tgatgggttg atctttggcg gtggctttat caggattact ttcaatgcct
     3421 agcacataag cggttttatc ccacagaaaa ttgggcttaa tgccagacgt gcgtttttca
     3481 gggcgaggta cgctcatcaa tttcggtttt ggtttcttat cttctgttaa atttgagatc
     3541 acattgacca aatttccctt tgaatctaat tctagtaccc aaccgatttt ttcttcactg
     3601 aaaccataag aaggcacttt agcttcgcct gttaagcgat cttcttgtgc tgcaaggcgg
     3661 taataatagc gagttaaggc ggtaataata gcgagttaag gcggagagaa tcatgatttg
     3721 acctcctctg cataaaatgg tggcacatca atcacgccat ttttcatttc cgcacggaag
     3781 aaacgaggtt gattaccgtg ctcaaaatca atgtcgtgca acatccagcc taaatcccga
     3841 tctcgctcat tttctgccaa aaaacactca ggtaacggtt catcgtcatc aatcaaggca
     3901 aaatttgcgg caaattcacg cacgcccata caaggctgct ggaaacattg cccctttttc
     3961 gcacggcgtt taaacatttc aatatgcttg gtgacggttt ccgcctcgcc tgccattttg
     4021 gtcagcactg cgtgagcttc gatcacataa ccgacatttt tcagcacagt agcagcacgt
     4081 tgttggcggt cgtcttcaat cacagtatat aaatccgcca ctgttttacg cttcattgca
     4141 ccactgattt tactggcaga aattttactg cctaactcat tacggcgaac agactcaaat
     4201 tgaatcggtt gtaatacata aattttatca atcacccagc gaattgccgg cttccaatgc
     4261 accgcagcca aaatcccccg tgccgcagac ggcgtaatca catcataaga cacccgttct
     4321 accttcattt caggacgggt aaaacaagca taatcacccc aaatatggag cctgattttg
     4381 tttggcataa tagaatgctc cttttaatta tgtaacacac acctccccga agggaggtga
     4441 aatttcttta gcttatatta taactgcttt ggtttcaacc cacgcactca gtaaaatgcg
     4501 aaaaatttta tggcttatta tattaagcgg tcggatttac ttagaaagtt gcaaagttat
     4561 ttgagtattt aaccgcttgt atttcaaaaa tagtcagaaa aataacagca ttctaggtac
     4621 aatatttttc taagaacgaa cttgtctccg caatgcctct actgcgtttg acacagcatt
     4681 tggtttgggc tcctggtttg cccaatcaga ccagaattgg cgtgtagcac tttctggggc
     4741 tatatgcggt ttagcaagtt tgattcgtgt aggtaataga atcgaatcgc ctagcaataa
     4801 cgcttcgcct aaatcaagta acggcaatat atcagtaatt ccaaccatag attccggcaa
     4861 taaacgcttt acaacacttt gatcttgacc atttgttaat cgtaaaacca agaaattatt
     4921 acattgactc aaaattgttc ggcttacatc tgaaggacgt tggctaacga ccaacaaaga
     4981 cacgccatat tttctacctt ctttcgcaat ccgctcaaag gcttccaatg cgcgtttttc
     5041 cgctgcttcc gctgaatctc gaacagggag atataaatgt gcttcatcgc aaataatcgt
     5101 tagtggatgg cgggaatctt tatccatcca aaattgaatg ttgtataaaa ggcgggcaag
     5161 aactcccgta acaatcggta aaacatccga cgggacttcc gaaaaatcaa taatttttat
     5221 ccctttttcg ctctcagaag cagaaaacag ttttgacaac atattttcca gccacccata
     5281 gctgttgcac tctactggcg gttggaacat aaaaccgcaa cgtctgtcat ctattttggc
     5341 tttcatcctt cccataaacc tagacaactt actataccaa ggaccttgct ttgttcctga
     5401 acctccctct ttaggtacca tctcagaatt atctttatct aaacgagcta acaataattc
     5461 aatagaatac ggaattggcg aatcaacagt aaacgtttcc aaaacatcat ttctttcttc
     5521 cttattcaat gtttgctctt ttaatgcgcg aacatgctca gtaaaacgcg ccgcctgatt
     5581 cggagcatta tcatcactgc gatctaaaat taaagccagc atttattccc gattgagcag
     5641 ccaataggga ataaataaac tgcgttcatc tgaaccatcc aaatctcccg gtccagcaat
     5701 acggtattgc tgagcgatag cgtttttacc ttcgcataga gtgctatatt caccgtgcat
     5761 atcgaataca ataagattag cagcttttaa agctgctgca cgttccaata ttaatgcaac
     5821 tgaccagctt ttaccggaac cagtactacc caaaatagca gcgtgccgtt gaaagaattt
     5881 atctccgttt gctatagcta cagccgtttc atcagccata aaatgaccta aagtcagccg
     5941 ttcattttct tttacgttag cggacaaaag attcataaat aattgcagat tgctgccact
     6001 aatatgaaag caatctctat caatttgcgg aaaacaatcc gctccccgct taaaagtgtt
     6061 gctcaaacta ccatcaacag ctttataagt accgattaat actgccttga taaagtcatc
     6121 ctgactttca cccagaggaa tttcaccctc attagatatt tcttctagca gcaatttttc
     6181 actgatagaa cgcgttaccc gttcgataat gccgataagg tactcactag ccgttctgcc
     6241 ttgaatcgca atcagattac ccacagcaat actagtcatt tgttgatgct cttcaacaga
     6301 aatcaataca cggctggtat ctactgttac aaccctgccc aaacgggcat tgtctttaaa
     6361 attcaatgtt tcatttgcca ttctctaaaa tctcttttac aaaaaactca acgtcccacc
     6421 aatgaacatt aggaataaaa gtttcttctt gcttgctaac cacccttgtt ccagccttat
     6481 ctccatcaaa aatcagagcc ttacaattcg gagcttcctt caccacgttt ctggcatttt
     6541 ctgaaagtcc tcttgtcaga agtagtgttt tcacaccact tttaattttg cctgtcaaat
     6601 gtgtttgaag gtggtcatca ttaaaaccat agccgataca taaaattgaa gaagcagaat
     6661 caatagcttc atttgctttt tctcgatgag catcaaatgg ttgtccatag ccttttttat
     6721 acttcccaac accaggcgta ataattaaag gctcttccac tttacctata caacttgtca
     6781 ttggaacgtc tccagccata aaccaatcta aactaccatg cggtttaaag attttgatat
     6841 gatctcgttg acatcctccc ctgcctaaag gcaggggatt cctactagct cccacagcaa
     6901 gctgtgggct actctcggtg ggtttctact gctgatctct attgagattc acttcataga
     6961 cgcttcgggt atgtcctacc ctgatattta ttatgcttgg actattgctt gcccacgtgt
     7021 aagaacattc attgctccca ctacatcggc attttccgta tagccacatt ctacgcattc
     7081 aaaattttcc tgtgtttggc gattttcttt tgctgtatag ttgcaacaag ggcaagtacg
     7141 actggtattt tgtggaggaa ctgctagcaa aaatccgcct aaccattgcg ttttgtagtc
     7201 cagttgtcgt ctaaattcaa accaagactg gtctaatatc actcggttta agcctgattt
     7261 ctgtttcaca tttttgccgt gtacttccgc tgtgcctttg gctgatttgg acatattcgc
     7321 cacttgcaaa tcttcaacat agatcatcgc gtggtttttg ctgatttgcg ttgagatttt
     7381 atgcaggaaa tcttttcggc agttggcgat tttgtgatgt aacttgccga ttttatcttt
     7441 cagtttttgc cagtttttac tgaacttcac tttatttttt agctgttttt gcagttttgc
     7501 caattttcct ttgtgggttt taaacgcatt taacggctca aaaaattctc cattggaaag
     7561 ggttgcaaaa cgagcaatgc ccatatcaat gccgatttct ccgcctttat gcgtgggaat
     7621 ttcctgttta aattccgttt gaatgctcac aaaaaagcgt ccgcatttca ttgaaacggt
     7681 gacatttttt acttccccaa caatttcacg actgttgcga taacgcaccc aaccgatttt
     7741 cggcagatac aagcggtcgt ttttctgctc gattttgcaa ccttgcggaa aacggaaact
     7801 ctcttttacg ccttttttct tgaatttggg caaatccgca cgcttttgaa agaagttttt
     7861 aaacgcactt tcaaggtctt ttaacgactg ttgaagcact tgtgaatggc aatctttaag
     7921 ccataccaat tcttttttcc attgcggaag caagttggcg agcttggtat aactgaattt
     7981 gtgatggtta tcttgttcgt actgctcatt ttgccacgcc aatgccttgt taaacacaaa
     8041 gcgagaacag ccgcaaaact gcttcatttt gcgaacttgt tccccatttg gtctgatttc
     8101 aaatttaaag gctttacgta gtaacattgc acgaattaga atacaaaaat tgtgcttatt
     8161 ctacacttgg cctatgaaaa aagaaacaga gattagacgt ggtcgccacg ttgtttttaa
     8221 cttacacgtt catttagttt tcgtgactaa atatcgcagg gaagttttta ctcaagcaat
     8281 tttgaacgac ttacactcaa tttttgcaac gatttgtgct gattttgaag ccacattagt
     8341 ggaatttgat ggtgaagatg atcacgttca tctacttgtg gaatatccgc ctaaagtcgc
     8401 tatttctcat cttgtaaaca gtttaaaagg cgtttcgagc cgaatgatta gaaagaaaaa
     8461 ctatccgagc attcgcaaaa aattatgggg aaatcaactt tggtcaccgt cttactttgc
     8521 tggtagttgt ggtggtgcac caatatcgat tattcgccaa tatatcgagc aacaacaaac
     8581 acctgattag ttagaaattt gaaacggctt tgccgtttat accttatatc cacgccctaa
     8641 aggacgtggt tttacggcac gattagataa accaattttg gacatttacc ttttactcta
     8701 atgtctctta tctgcaattt cctactcaaa tcaggattat gttttcccca ataacgaccg
     8761 accatcattg aatctattcc ccaattttca tactctgccg caaactcaat caatctatca
     8821 taattagtag taattacacg ggcaatttta ggatttgaag ctgaaatatg gggaagcaaa
     8881 taagaaaatt tgagcttctg attctcagca atacatttgt ttatcgcttt ttgctcttcg
     8941 gagagtacat actcggctgt tattttaata atagcggctt ctatcgtctc atttgcttga
     9001 tgcttcaaca atgcaccttc caagccttca gaatccagac aatcagaaat ggtattccat
     9061 gttaccttat ctatatcatc aagacattca ggcattcgtt cttttaatct atcagctaat
     9121 gctcccattc ccggcaaacc ttcggcacag gacaaacccg aaccgacaat aatgaccaaa
     9181 ttgtcagtaa aaatagtttg caaccatttt tttaattcat cttcatctaa taatggttct
     9241 ctcattttac gtttcccttc caaatatcta ttcttacaaa ctagctgttt actacgagac
     9301 g
//
LOCUS       DANLZC010000100         9237 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.100, whole genome shotgun sequence.
ACCESSION   DANLZC010000100 DANLZC010000000
VERSION     DANLZC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.100"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            157..873
                     /locus_tag="PXE44_001837"
     CDS             157..873
                     /locus_tag="PXE44_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5589325.1"
                     /translation="MKKTLLTLLFGCVVTAQAQDIKFVMEPSYPPFEMTEEKGEIIGF
                     DVDIANAICKEMNANCTFHSQPFDSLIQSLKQKQFDAAISGMGITEPRKKQVLFSEPY
                     FPSSAAFIAKKDTDFAKVKTIGVQNGTTYQHYLAKEKKEYNVKSYASYQNAILDVQNG
                     RIDAIFGDVPVLAEMARKHEGLDFVGEKINNPNYFGDGLGIATHLKNQVLVDQFNAAL
                     KTIKENGEYQKIYDKWMGGK"
     gene            complement(943..1821)
                     /locus_tag="PXE44_001838"
     CDS             complement(943..1821)
                     /locus_tag="PXE44_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDL5589326.1"
                     /translation="MKNLYKWPAAQSLYPNQGKKSYAGKRFRYRLRSWLHHSKIKQFE
                     QFVTQNPQLIPLLNARPNYSYPVAHRFLDKRFSAKQRLQKITDNLLFLPQKLAHLPPL
                     WEQAVNFGEIIADFELWLNINEHQPMEGFWALELRHRPTNQLVYLLTFGKLDEALLIA
                     VIQGPNFEGSKELVKQLTKSCHGLRPAYLMVETMKALTAVLGYQSLLGIPQKYQNKSR
                     WVQSKRYVVDYDAIFAESGGKPGNYWQLPLELESKKIDEIPSKKRSMYRKRYEMLANL
                     KQTMELMLKTYTGLNG"
     gene            complement(1897..3222)
                     /gene="hslU"
                     /locus_tag="PXE44_001839"
     CDS             complement(1897..3222)
                     /gene="hslU"
                     /locus_tag="PXE44_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDL5589327.1"
                     /translation="MMSMTPREIVSELDAHIIGQKDAKRAVAIALRNRWRRMQLPEDL
                     RQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LADVSMKLVRQQAVEKNKMRAQDAAEDRILDVLLPPAKDQWGNVQESDNNSTRQTFRK
                     KLREGQLDDKEIEIDVAAQVSVEIMTPPGMEEMTSQLQSLFEGMSPSKTKKRKMKIKD
                     ALKVMLDEEAAKLVNPEELKQQAIEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQ
                     RDLLPIIEGSTVNTKHGMVKTDHILFICSGAFQVARPSDLLPELQGRLPIRVELKSLT
                     KEDFERILTEPNASLTLQYRELMKTEGVEIEFTKDGISRIAESAFRVNEKTENIGARR
                     LHTVLERLMDGISFDASERRGEKIIIDENYVSEALNDVVENEDLSRFIL"
     gene            complement(3479..4000)
                     /gene="hslV"
                     /locus_tag="PXE44_001840"
     CDS             complement(3479..4000)
                     /gene="hslV"
                     /locus_tag="PXE44_001840"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDL5589328.1"
                     /translation="MTTIVCVRKDGKVAIGGDGQATLGNCIEKGTVRKVRRLYKDKVV
                     TGFAGSTADAFILRDLFEKKLELHQGHLVKSAVELAKEWRTERALRRLEAMMIVANDS
                     EFLLVSGSGDVIEPEQDVLAIGSGGNYAKAAALALLRTENNLSAKEIVAEALKIAGDI
                     DIYSNYNHVIEEV"
     gene            complement(4061..4774)
                     /gene="sfsA"
                     /locus_tag="PXE44_001841"
     CDS             complement(4061..4774)
                     /gene="sfsA"
                     /locus_tag="PXE44_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL5589329.1"
                     /translation="MKFPPLSHGILLRRYKRFLADIQLPNGEQITLHCPNTGAMTGCA
                     NPGDTVWFSTSHNPKRKYAHTWELTQTQAGDFICVNTLRANQLVQEALENQWISELAD
                     YQHILPEQKYGSENSRIDFLLKSDLLPDCFVEVKSTTLLTENGVGMFPDAKTERGQKH
                     LRELTKIAEQGLQAVIFFAILHTGIERFEVAKQIDPKYAELFAQAIAQKVQVLSYKMA
                     ISLENGQPIAATLTKKCEF"
     gene            complement(4790..5659)
                     /locus_tag="PXE44_001842"
     CDS             complement(4790..5659)
                     /locus_tag="PXE44_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5589330.1"
                     /translation="MLYLLIATLIWASSFIVGKVAISAFDPVQIMQIRLTLAAFIALP
                     FFIPAYKKIQKNQYIKVWLISFLTFPLYVILQFSGLKLTSAASAVALLGLNPLLTVLV
                     GYFCFKKQATRLDFILSLFACLGILIMASGSDDSGSINVLGLLLVTLGSLSFIVGMYL
                     SKEILQSVKVIDLTKIMLVQGAITALPITLLFAENWQLPTSLSAWASVAYLGIICTSL
                     AILLWNKGISQSSPILAGILLALEPVFGLLMALVFLSEQLSLVTWVGIIIVICTALLS
                     VLLPMMKRYKRLI"
     gene            complement(5660..6052)
                     /locus_tag="PXE44_001843"
     CDS             complement(5660..6052)
                     /locus_tag="PXE44_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5589331.1"
                     /translation="MKSPILGFHHIALIVSDYEKSKHFYTRILGAEIIAETYREQRAS
                     YKLDLRFKDGSQIELFSFPETPPRFTSPETCGLRHLAFRVENIEYAIAFLEQHQLPHE
                     GIRIDEITGKRFTFFRDPDDLPLEFYEV"
     gene            6136..7281
                     /gene="mutY"
                     /locus_tag="PXE44_001844"
     CDS             6136..7281
                     /gene="mutY"
                     /locus_tag="PXE44_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL5589332.1"
                     /translation="MNSNSPFPLAKAKASDPFAKAVLAWFEQYGRKHLPWQQNKTLYQ
                     VWLSEVMLQQTQVATVIPYFARFMERFPTVIDLANASIDEVLHLWTGLGYYARARNLH
                     KAAIQIRDEFGGEFPTRFDDVLALTGVGRSTAGAILSSVLDAPHPILDGNVKRVLSRY
                     FAVEGWAGEKAVENRLWQLSESVTPDTQVADFNQAMMDLGAMICTRTKPKCLLCPLQE
                     NCKTNAMQAWDSFPAKKPKKTLPERQTYFLVLKSGSKVLLQKREAKGIWGGLFVFPQF
                     ESLEALKRSDFMQNLQISQQLTAFRHTFSHFHLDITPVLVETDLKKTSENSPLVVEEK
                     AGNYLSKVSSNADYWYDLHQHNEVGLATPVKRILDELAFSVKNEIKD"
     gene            7285..7554
                     /locus_tag="PXE44_001845"
     CDS             7285..7554
                     /locus_tag="PXE44_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDL5589333.1"
                     /translation="MARTVFCEYLKQEAEGLDFQLYPGELGKRIFDSISKQAWSEWIK
                     KQTMIVNEKKLSMMNPEHRKLLEQEMVNFLFEGKEIIIEGYKPVE"
     gene            7571..8662
                     /gene="mltC"
                     /locus_tag="PXE44_001846"
     CDS             7571..8662
                     /gene="mltC"
                     /locus_tag="PXE44_001846"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDL5589334.1"
                     /translation="MKKYAKYLPMLAIIPFLIACGSNKSTKKPKSTRVDYSKDTNGLD
                     ILAGQFSHNIDDIWGSNELLVASKKDYVKYTDKFYTRSHISFEEGVITIETLGNQNHL
                     RNSIIHTLLMGRDPKGIDLFASGDAPISSNPFLNGQVEDQFGRNITNVAIANDFATYL
                     LQNKVQTRRLKNGQTVSFVTIKMVAGHIEVRARQYLPLVRKMAKQYGIEPSLILGIME
                     VESAFNPYAISYANAIGLMQVVPRTAGRDIFARKGMGGQPSREYLYNPSNNIDAGTLY
                     LTILRDEYLDGIEDPVAKRYAMISAYNSGAGAVLRVFDSDRYSAIDRINDLSSDAVYR
                     ILTTAHPSSQARNYLRKVTTAREKYRNIR"
     gene            8794..8869
                     /locus_tag="PXE44_001847"
     tRNA            8794..8869
                     /locus_tag="PXE44_001847"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8827..8829,aa:Phe,seq:gaa)
     gene            8875..8950
                     /locus_tag="PXE44_001848"
     tRNA            8875..8950
                     /locus_tag="PXE44_001848"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8908..8910,aa:Asn,seq:gtt)
     gene            9121..9196
                     /locus_tag="PXE44_001849"
     tRNA            9121..9196
                     /locus_tag="PXE44_001849"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9154..9156,aa:Phe,seq:gaa)
BASE COUNT         2675 a         1873 c         1965 g         2724 t
ORIGIN      
        1 tttttttgag atagaggaat caagaggatt gagaaactac atctcaattt gatgtgtttg
       61 tatgacataa aacaaacttt atgaaaagaa gacttttttt aagaaagtaa ctgcgttaaa
      121 gtggtaatcc ttataatgaa aacacaggga ctttttatga aaaaaacact tttgaccctt
      181 ttattcgggt gtgtggttac tgcgcaggca caagatatca aatttgtgat ggagccaagc
      241 tatcctccat ttgaaatgac cgaagagaag ggagaaatta tcggttttga tgtggatatt
      301 gcaaatgcta tctgtaaaga gatgaatgca aattgtacat tccatagcca accttttgat
      361 agcttaatcc aaagcctaaa acaaaaacaa tttgatgctg caatttcagg tatgggtatt
      421 acggaaccac gtaagaaaca agtattattt agcgagcctt actttccaag ctctgctgcc
      481 ttcattgcga aaaaagatac agattttgct aaggtgaaaa cgattggtgt gcaaaatggg
      541 actacttacc aacactattt agcaaaagag aaaaaagagt acaatgtgaa atcttatgcc
      601 tcttatcaaa atgctatttt agatgtgcaa aatgggcgaa ttgatgctat ttttggcgat
      661 gtgccggtat tggctgagat ggcaagaaaa cacgaagggc tagattttgt aggggagaaa
      721 atcaataatc caaactattt tggtgatggt ttaggcattg caactcactt gaaaaaccaa
      781 gtattagttg atcaattcaa tgcagcatta aaaacgatta aagagaatgg cgaatatcaa
      841 aaaatctatg ataaatggat gggcggcaag taacttgtaa gcggtcattt tttgcaaagt
      901 tttgcaaagt tttgcagatt ttgaccgctt gtattctttg atttacccat ttaagccagt
      961 ataagtttta agcataagct ccatggtctg ctttaaatta gctagcattt cataacgctt
     1021 gcgatacatt gagcgttttt tgcttggaat ttcatctatt tttttacttt ccaattcaag
     1081 gggtaactgc caataatttc ccggctttcc gcccgattcc gcaaaaatag catcataatc
     1141 cactacatag cgcttacttt gcacccagcg ggatttattc tggtatttct gtggaatgcc
     1201 tagtaaactc tgatagccaa gaacagccgt taaagccttc atcgtttcaa ccattaaata
     1261 ggccgggcgt aagccgtggc aacttttggt taattgtttc accagctctt ttgaaccttc
     1321 aaaattcgga ccttgaatta cggctatcaa caaggcttca tctaacttac caaatgttaa
     1381 taaataaact agctgattag tcggtctgtg gcgaagttct aatgcccaaa aaccttccat
     1441 cggctgatgt tcattgatgt taagccataa ctcaaaatct gcaataattt caccaaaatt
     1501 gaccgcttgt tcccatagag gtggtaaatg agccaatttt tgcggtaaaa acagcaggtt
     1561 atctgtgatt ttttgtaagc gttgttttgc actgaaacgc ttatctaaaa agcggtgagc
     1621 cacggggtag ctgtaatttg gtcgagcatt taagagcgga atcagttgtg gattttgtgt
     1681 aacgaactgc tcaaattgct tgattttgga atgatgcaac caagagcgaa gacggtaacg
     1741 aaaacgtttc cctgcgtaag acttctttcc ctgatttgga taaagcgatt gtgcagcagg
     1801 ccatttgtat agatttttca tttttacaac taacaatatg aaaagcggct aattcgcaag
     1861 atttttcgct aaaataaccg cttgtataac ttaaaactac aaaataaagc gacttaaatc
     1921 ttcgttttcc accacgtcat ttaacgcttc ggaaacatag ttttcatcaa tgatgatttt
     1981 ttcaccacgg cgttcgctgg catcgaacga aatgccgtcc atcaaacgct caagcacggt
     2041 gtgtaaacgt cttgccccga tgttttcggt tttctcgttc acacggaatg cactttctgc
     2101 aatacggctg ataccgtctt tagtgaactc aatctccacg ccttcagttt tcatcaactc
     2161 acgatattgc aaggttaatg aggcattcgg ttcggttaaa atacgctcaa aatcttcttt
     2221 ggtaagcgat tttaactcca cacgaatcgg caagcgaccc tgtaattctg ggagtaaatc
     2281 agacgggcga gccacttgga atgcccccga gcagataaac agaatatggt cggttttcac
     2341 cataccatgc ttggtattta ccgttgagcc ttcaatgatt ggaagtaaat cacgctgaac
     2401 accttcacga gaaacatctc cacccgaatg ctcgcctttt ttacagattt tgtcgatctc
     2461 atcaataaag acaataccgt gctgttccac cgcttcaatc gcttgctgtt tcagctcttc
     2521 aggatttact aatttcgccg cttcttcatc aagcatcacc tttaacgcat ctttgatttt
     2581 cattttgcgt tttttggttt tgcttggcga cattccctca aacagcgatt gtaattgcga
     2641 ggtcatctct tccattcccg gcggggtcat aatctcaacg ctcacttgag ctgccacgtc
     2701 aatttcaatc tctttatcgt ctaattgccc ttcacgcaat tttttgcgga aagtttggcg
     2761 ggtagaatta ttgtcggatt cctgcacatt tccccactga tctttcgccg gtggcaataa
     2821 tacgtctaaa atgcgatctt ccgctgcatc ttgtgcacgc attttatttt tttcgaccgc
     2881 ttgttggcgg actaatttca tcgacacatc agctagatca cggataatcg aatccacttc
     2941 tttacccaca taccccactt cagtgaattt ggttgcctca actttgataa aaggggcatt
     3001 ggcaagcttc gccaaacggc gggcgatttc ggttttaccc acaccggtcg gaccgatcat
     3061 taaaatattt ttgggggtca cttcttggcg gagatcttcc ggcaactgca ttcttctcca
     3121 gcggttacgc aaagcaattg ctaccgcacg ttttgcatct ttttgtccga taatgtgtgc
     3181 gtctaattct gagacgattt cacggggtgt cattgacata atgttattcc ttaaattaat
     3241 taccctctcc ccttgtggga gagggacaga tttttctttg ttcagaagaa aaatcaggga
     3301 gagggtaaca agcggtcttt tttcttaaac gttttgcatt aacccctctc tctgaaaaat
     3361 atttctgaac gaaatatttt tcggtctcaa tactcgctac gctcgctcct gtggagccgt
     3421 tggcaaagcc aacgttcaaa tgtaaacatt tgtcccacaa ggggagagag ttatttaact
     3481 aaacttcttc aatcacgtga ttgtagttac tgtaaatatc aatatcgccg gcgattttta
     3541 acgcttccgc aacgatttct tttgcagata aattattttc ggtgcgtaat aacgcaagag
     3601 cagcggcttt cgcatagtta ccgcctgaac caatcgccag cacatcttgc tccggctcaa
     3661 tcacatcgcc cgaacctgaa accaataaaa actcgctgtc attggcgaca atcatcatcg
     3721 cttctaaacg gcgtaaagca cgctcggtac gccactcttt tgccagttcc actgccgatt
     3781 tcactaaatg cccttggtgt aactccaatt ttttctcgaa taaatcacgc aaaataaagg
     3841 catcagcggt cgagccggca aagcctgtca ccactttgtc tttatacaaa cggcgaactt
     3901 tccgcaccgt gcctttctcg atacaatttc caagcgttgc ctgaccatca ccaccgatag
     3961 ccacttttcc gtctttacgc acgcatacaa tcgttgtcat actaaatcct tctttttaaa
     4021 aactttggct tatatgggga tatgctgtgt gttttcaagc ttaaaactca catttctttg
     4081 ttaaggttgc cgcaataggt tgcccgtttt ccaatgaaat tgccatttta taggagagaa
     4141 cctgcacttt ttgtgcaatg gcttgagcga ataattcggc atatttgggg tctatctgct
     4201 ttgccacttc aaaccgttca atacctgtgt gcaaaatagc gaaaaaaatg accgcttgta
     4261 agccctgttc agcgattttc gtcagctccc gcaaatgttt ttgcccccgc tcggttttgg
     4321 catcggggaa cattcccaca ccgttttcgg ttaaaagggt ggtagatttc acttccacaa
     4381 aacaatccgg cagaaggtcc gatttgagca aaaaatcaat ccgactgttt tcactgccat
     4441 atttttgttc aggcagaatg tgctgataat ccgcaagctc gctaatccat tgattttcca
     4501 aggcttcttg tacaagctga ttggctctca aggtattgac acaaataaaa tcccccgctt
     4561 gggtttgggt tagttcccaa gtgtgagcat atttacgttt ggggttatga gaggtggaaa
     4621 accaaactgt atcaccgggg ttggcacagc ctgtcatcgc accggtattt gggcaatgga
     4681 gggtaatttg ttcgccattt ggcaactgaa tatcggcaag aaaacgttta taacggcgaa
     4741 gtaaaatgcc gtgggaaaga gggggaaatt tcatttgcaa aaaatcctgt taaattaacc
     4801 gcttgtatct tttcatcatc ggtagcaaaa cggagagtaa ggcggtgcaa ataacaataa
     4861 taatcccaac ccaagtcact aaactgagct gttctgataa aaagaccaat gccattagca
     4921 gtccgaatac cggttcaagt gcgagtagaa tacctgcaag aataggcgaa gattggctga
     4981 tgcctttgtt ccataacaat attgccaagc tggtacaaat aatgcctaaa tatgctacgc
     5041 tcgcccatgc tgagagactg gttggcagtt gccaattttc tgcaaagagt aaggtaatag
     5101 gtaaggcggt aattgcccct tgcaccagca tgatcttggt taaatcgatc acttttactg
     5161 attgcagaat ctctttgctg aggtacattc ccacaataaa acttaaactg cctaaggtta
     5221 caagcaataa tcctaataca ttgattgaac cgctatcatc gctacccgat gccataatta
     5281 aaatccccaa gcaggcaaat aaacttagga taaaatcgag cctggttgcc tgttttttga
     5341 aacaaaaata gccgactaac acagttaaaa gcgggtttag cccgagtagt gccacggcac
     5401 ttgctgcgga agttaatttc aagccggaaa attgtaagat cacgtacaga ggaaaagtga
     5461 gaaaactaat cagccagact ttaatgtact gatttttctg gattttttta tacgcaggaa
     5521 taaaaaacgg cagagcgata aacgccgcta acgtcagccg aatttgcata atttggacag
     5581 ggtcaaaggc tgaaatcgcc actttaccta caatgaatga actcgcccaa atcagggttg
     5641 caatcagtaa atataacatt tagacctcgt aaaactctaa cggcagatcg tccggatctc
     5701 ggaaaaacgt gaagcgtttg ccggtgatct catcaattcg gatcccttca tgcggtaatt
     5761 gatgttgttc taaaaatgcg atggcatatt caatattttc cactcgaaat gccaaatggc
     5821 gaagcccgca ggtttcgggc gaggtgaaac gaggcggcgt ttcaggaaaa ctgaataatt
     5881 caatttgtga gccatcttta aagcgtaaat ctaatttata gctggcacgt tgctctcggt
     5941 aggtttctgc gatgatctct gcccctaaaa tgcgggtgta gaaatgtttg gatttttcgt
     6001 aatcggaaac aattaatgca atgtggtgga aacctaaaat gggcgatttc atcttttttc
     6061 cttaatgtgt aaaatttttg gtaaaactga ccgcttgcag tatgataatt tatttttcgg
     6121 atttcaagat ttttaatgaa cagcaattcc ccatttcctt tagcgaaagc aaaagcttct
     6181 gatccgtttg ccaaagctgt tttagcttgg tttgagcaat atgggcggaa acatttacct
     6241 tggcagcaaa ataaaacgct gtatcaggtt tggttgtcgg aggtaatgtt gcaacaaact
     6301 caagtagcga ccgttattcc ttattttgca cgttttatgg aacgatttcc caccgtaatt
     6361 gacttagcta atgcctcgat tgatgaagta ttgcatttgt ggacagggct gggttattac
     6421 gctcgggcaa gaaatttgca taaagcggca attcaaattc gagatgagtt tggcggtgaa
     6481 tttcctaccc gatttgatga tgtgttggca ttaaccggag tgggcagaag tactgcaggg
     6541 gcgattttgt cttctgtgtt agatgcccca cacccgattt tagatggcaa tgtcaaacgg
     6601 gtgttaagcc gctattttgc agtagaaggc tgggcgggtg aaaaagcggt ggaaaatcgc
     6661 ctttggcaat taagcgaaag tgtgacgcct gatacccaag tggcagactt taaccaagcg
     6721 atgatggatt taggggcgat gatttgcacc cgtacaaagc ctaagtgctt gctctgcccg
     6781 ctacaagaaa attgtaaaac gaatgcaatg caagcgtggg atagctttcc tgccaagaaa
     6841 ccgaaaaaaa ccttgccgga gcgtcaaacc tattttttag tgctgaaaag cggctcgaaa
     6901 gtgctgttac aaaaacggga agcaaaaggc atttgggggg gcttatttgt gtttccgcaa
     6961 tttgagagct tagaggctct caagcggtct gattttatgc aaaatttgca aatttcacag
     7021 caattaactg catttaggca tacttttagc cattttcatt tggatattac cccggtttta
     7081 gtagaaaccg atttgaaaaa aacaagtgaa aattcaccgc ttgttgtcga agaaaaagca
     7141 ggaaattatc tttctaaggt atcttcaaat gcggattatt ggtatgattt gcaccaacat
     7201 aacgaagtgg gtttagcaac gccggttaaa cgtattttag atgagctggc attcagtgtg
     7261 aaaaatgaga ttaaggatta aattatggct cgtaccgtat tttgcgaata tttaaaacaa
     7321 gaggcagaag gtttagattt tcaactttat ccgggggagc tgggtaagcg tatttttgac
     7381 tcaatcagca agcaagcgtg gtctgagtgg attaaaaaac agacaatgat cgtcaatgag
     7441 aaaaaactca gtatgatgaa ccccgaacac cgtaaactgc tcgaacaaga gatggtcaat
     7501 tttttatttg aaggtaaaga gatcatcatc gaaggctata agccggtcga ataataaagg
     7561 ttaaatcaca atgaaaaaat atgctaaata tttgccgatg ttggcaatta ttccgtttct
     7621 tattgcttgt gggagcaata aatcgactaa gaagccaaaa tctacccgag tagattattc
     7681 taaagatacc aatggtttgg atattttggc agggcagttt tctcacaata ttgatgatat
     7741 ttggggaagt aacgagctgt tagttgccag taagaaagat tatgtgaaat atacggataa
     7801 gttttacacc cgtagccata tttcgtttga agaaggggtg attacgattg agactttagg
     7861 gaatcagaat catctacgca attcgattat tcatacttta ttaatgggca gagacccgaa
     7921 agggattgat ttatttgcct caggcgatgc accgattagc tctaaccctt tcctaaacgg
     7981 acaggttgaa gatcaattcg ggcgaaatat taccaatgtt gccatcgcca atgattttgc
     8041 gacttatttg ttgcaaaata aggtgcaaac acgccgtttg aaaaatggtc aaactgtgag
     8101 ctttgtaacg attaaaatgg tggcaggaca cattgaagta cgtgccagac aatatttacc
     8161 gctggtgcgt aaaatggcga agcagtatgg tattgagcca agtttgattt tagggattat
     8221 ggaagtcgaa tcagcattca acccttatgc catcagctat gccaatgcga ttggcttaat
     8281 gcaggttgta cctcgtaccg ccgggcgtga tatttttgcc cgtaaaggta tgggagggca
     8341 gccaagtcgt gaatatcttt ataatccatc aaataacatt gatgcaggta cgctttattt
     8401 gactattttg cgtgatgaat acttagatgg aattgaagat cctgtggcta aacgctatgc
     8461 aatgatttct gcatacaata gcggagcagg tgcggtatta cgagtgtttg attcagaccg
     8521 atactctgcg attgatagaa ttaacgatct ttcgtctgat gcggtttatc gaattttaac
     8581 cactgctcat ccatcttctc aagctagaaa ttatttaaga aaagtaacca ccgccagaga
     8641 gaaatatcgc aatattcgtt aatgtgtgaa ttatgctgtt ttttagccga aaagtataaa
     8701 aaaagcacat ttgagcagcg gtttttttaa atttgcaaaa aaacgtttga cgttgcaagc
     8761 tgaaatcagc ataatgcacc acgcaacgac gttgcctcga tagctcagtc ggtagagcag
     8821 gggattgaaa atccccgtgt cggtggttcg attccgcctc gaggcaccat caattcctcc
     8881 ttagttcagt cggtagaacg gtggactgtt aatccatatg tcgcaggttc gagtcccgca
     8941 ggaggagcca tattctgaag ccctgatgag taatcatcag ggttttcttt ttgagctttc
     9001 cggctaacta ctaagaaatt gctcaatttt tagtattatg cgttcaaaaa cgctaaattt
     9061 ttgagaattg tatttgacga agcccgaaaa aatcagcata atgcaccacg caacgacgtt
     9121 gcctcgatag ctcagtcggt agagcagggg attgaaaatc cccgtgtcgg tggttcgatt
     9181 ccgcctcgag gcaccatcaa ttcctcctta gttcagtcgg tagaacggtg gactgtt
//
LOCUS       DANLZC010000101         9000 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.101, whole genome shotgun sequence.
ACCESSION   DANLZC010000101 DANLZC010000000
VERSION     DANLZC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.101"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            70..954
                     /gene="oxyR"
                     /locus_tag="PXE44_001850"
     CDS             70..954
                     /gene="oxyR"
                     /locus_tag="PXE44_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDL5589335.1"
                     /translation="MNIRDLEYLIALAEHKHFRRAAESCNVSQPTLSGQIRKLEGELG
                     TILLERTSRKVLFTQAGLTLVEQAKVVLREVKILKEMASNQGKEMSGPLHVGVIPTLG
                     PYISPIIVPALKAEFADLELYIYELQTNQLLDQLESGQLDCGIISFVKESESFIEVPI
                     FNEEMYLVVPEQHEWAALKKFDMNLLKNRELLFLDSGHCLRTNTLDYCLSIGAKENPH
                     FKATNLETLRNMVAANVGLALIPKLAAKPIKGVHYIPLENPKPFRAVGMVYRPGSPLR
                     ARYERLAQAITKIMQSEA"
     gene            957..1589
                     /gene="fabR"
                     /locus_tag="PXE44_001851"
     CDS             957..1589
                     /gene="fabR"
                     /locus_tag="PXE44_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDL5589336.1"
                     /translation="MANGMSVRAIQKEKTRRALVEAAFNQLSSEKNFSNLSLREVARE
                     AGVAPTSFYRHFKDMDELGLAMVDESGLLLRQLMRQARKRIANGGSVVVVSVETFFEL
                     IADRPNVFQLLLRESSGTSQAFRTAASREIQHFVAELADYIMSKDNHSNRELAYIQSE
                     GLVTLVFTAGSHALDMNNQEREKLKQRVIMQLRMLIRGSTYYANKQASST"
     gene            complement(1616..1915)
                     /locus_tag="PXE44_001852"
     CDS             complement(1616..1915)
                     /locus_tag="PXE44_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HDL5589337.1"
                     /translation="MKKADIQKMQDCYNQWVELLPELEKGIEQWKRASALLEPLSRFY
                     ASPEWRELHDSFDEQLDTKGNYSILSEDALWNALSEHHQLALEWLKLSTAYITKE"
     gene            complement(2004..5819)
                     /locus_tag="PXE44_001853"
     CDS             complement(2004..5819)
                     /locus_tag="PXE44_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5589338.1"
                     /translation="MKGSGLATVGAPETGDNGQTIYTVDVAKAGAPTVTRGNVADAIN
                     NSEKTSSVVAGSKAVTVKAGKEDDKGNTEYTVDVATDKSIGRDNDGNMTVNTDNVTIV
                     KDPTTGEVKANTTTLNNSPEGKVTEPTGDDAKKLVTAGDIANAINNSGFNVTAGDGVD
                     GGETKGKKTQLIKPSETVTFDAGKNMTLTQADGKFTYTTKDNVAFNSIDMSNGKPDTT
                     GSITNLKSGVGGTFADKAAPTDAERKAIADNINNATGDTLNNAVNVGDVQAAMKAATT
                     KVEGDKGVTVTSKTNDDGSTTYTVEAKTDGTTIKVNDKGEITANTSELTNNPDGKVEE
                     PTEPNALVTAKTVADAINNAGFNIKANGDEKSLVKTGDTVQFLNGKNIEITRDGNNIT
                     VSTAKNVNFDSVQFGNEGPKITNNGGNINVGDKDGNAVKVTNVAAGDVNADSKDAVNG
                     SQLYTFAMASREEVKSTDKSVTVNTTKNADGANVFDLSVNTDDVTIVKDPTTGAIKAN
                     TTALNDANNDGRIDEPTADDAKKLVTAGDITNAINNSGFTLKTSAVEGGEKLSGGDEL
                     INPGKAVEMVAGKNLTVKQEADGKVIYATKDDVKFSSVTSNTVTVPTDEADPANNPIT
                     INKDGINAGNKAISNVASNLIPVTADDKVQPADNNPTNLADKLSNAATLGDVLNAGWN
                     LQGNGKAVDTVVHNDTVDFINGKGTTVTVENKDGKNTIKVDSPIEFVNQDPTDSSTPS
                     NTAKFTGEAPVQLGNVASSVRNEDGSTPEGKDRAEAIKNAEGDKLNNVVNLGDLQAAT
                     NAATTKVGGNRGVTITPSTNADGSTTYNVEAKTDGTTIKVDNEGNITANTSELGNNED
                     GTVKAPTQPNALLIAQTVADAVNNAGFNIKSAGNKAAGDQAATKLVKTGEEVVFEAGD
                     NLTVKRDGNQFTFATAKDVSFNSVQFSENGPKITNDGDNIKVGDKDGKPTKITNVADG
                     DISPVSTDVINGKQLNNYAKVNGNNIGTDEDGSINIVNGNGTTITSDKAGEVKVNVNI
                     TDLTVADNGKINVQDPNGTGSRFVNATTVANAVNNVSWNVDSKAVGTGAVEGDKAPAK
                     VKAGSTVSVNAGNNIKVTRKGSDVTVAVSDTPEFTSKTGDTLVNNNGVTINNGSAGKA
                     VSLTKDGLNNGGNRITNVKAGEADTDAVNVGQLKGAVNHLNNKIHRNNREARAGIAGS
                     NAAAALPQVYIPGKSMVAAAGGTFKGENALAVGYSRSSDNGKLILKLQGNANSRGDFG
                     GGVGVGYQW"
     gene            complement(5782..6267)
                     /locus_tag="PXE44_001854"
     CDS             complement(5782..6267)
                     /locus_tag="PXE44_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589339.1"
                     /translation="MDTGSITVDNTTGAVTTPAEEDKVATTKTVSEAIQKAGWNAKSG
                     GNKADGDQEAAELINPGEKVIFAAGDNLKVKRVGTIFTYETAKDVKFDSVTFGDNGPK
                     ITNKDGNVNIAGNDGNPTKITGVKAGEADTDAVNVSQLKQAAASQNRSERFRFSNCWC
                     T"
     gene            complement(6276..8435)
                     /locus_tag="PXE44_001855"
     CDS             complement(6276..8435)
                     /locus_tag="PXE44_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5589340.1"
                     /translation="MGARATSANAIALGTDTAATGNRATAFGAGALATGNRSTVMGWR
                     SAASGTRSFAMGSGAMGDKSVANATYSLALGSSANANATSTATRGTAIGYNATVSGVN
                     ATALGVSAVSSNQSTVAIGDSAKASAGFAIALGSGANVALNNSVALGSNSTVSNAVAV
                     PSATVNGITYSGFAANTTAAGNVVSVGSNTIKRQIQNVAAGQISATSTDAINSSQLYM
                     AMNATGNLANSTKNILGGNATVKPDGSVTYTNIGGTNKNTIEEALKAVKTEVVAGSNV
                     NITNATGANGQTIYTVNAYNTTANSSSPDYITVTGKAATAANTTNYEIGLTKKAIDDF
                     TKDTQATVVSNDGTVTVKSTERNANGTVIYDLSVNIPAQASQIQYFSVNSTVPENQAN
                     DGAKSRNSIAIGPNATATGGEQAAVALGTNSNANGNGALSLGVATVSKGIQATAVGHS
                     ANATANGTTALGRQTNATAGDATAVGSNANATAEKASAFGVAANASANASLAVGANSI
                     ASAQSAVAVGTRANATAQFATALGMGAQATLNSSVALGSESVVRAATPTENATVGGIT
                     YNGFAGVNKDTNYVVSVGSAGKERQIQNVAAGQISATSTDAINGSQLYMAMNATSNLA
                     NSTANNFGGGSVVNPDGSVTQPKYNVTNAAGTQYGNTATNVGDAITNLNNYVNQGFNI
                     KDNAGETKGTVTPNESVQFVNGKGTVSNVTQEADGVK"
     gene            complement(8664..8864)
                     /locus_tag="PXE44_001856"
     CDS             complement(8664..8864)
                     /locus_tag="PXE44_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HDL5589341.1"
                     /translation="MNQIFKVIWNQATQSWVAVSELQRAKGKTKSVSSASKATLALLA
                     GASAVLGANDAVAVVSYRFNNK"
BASE COUNT         2432 a         2066 c         1598 g         2904 t
ORIGIN      
        1 ttatactcac aaatatggca taattcagcc tatcagaaca ataggaaaaa tttaaattgg
       61 aggaatcaag tgaatattcg agatttagaa taccttattg cacttgcaga acataagcat
      121 ttccgtcgtg cagcagaatc ttgtaatgtt agccaaccta ctttaagcgg gcaaattcgc
      181 aaattagaag gcgaactagg cactattctg cttgaaagaa ccagtcgcaa agtcctcttt
      241 acgcaagcag gcttaacatt agttgaacaa gccaaggtag ttttacgtga agtaaaaatt
      301 ctaaaagaaa tggcaagcaa ccaaggtaaa gagatgtctg gcccgcttca tgttggtgta
      361 atcccaacct tggggcctta catttcgcca attattgtgc ctgcattaaa agcagaattt
      421 gcagatttag agctgtatat ttacgaatta caaaccaacc aattattaga tcaacttgaa
      481 tccggccagc ttgattgtgg cattatctca tttgtaaaag agagtgaatc ttttattgaa
      541 gtgcctatct ttaatgaaga aatgtacttg gtagttccag aacaacacga atgggcagcg
      601 cttaaaaaat tcgatatgaa tttattaaaa aaccgtgagt tgctcttttt agacagtggc
      661 cattgtttac gcactaatac gctagactat tgtctatcca ttggagcaaa agaaaatcca
      721 cactttaaag ctaccaacct tgaaacctta cgcaatatgg tagcagcaaa tgttggccta
      781 gcattgattc ctaaattagc cgctaaaccg attaaaggag tacattatat tccacttgaa
      841 aacccgaaac cgtttcgagc tgtgggaatg gtgtatcgac caggttcacc attacgcgcc
      901 cgctatgagc gtttagctca agccattact aaaattatgc aatccgaggc gtaaaaatgg
      961 caaacggtat gagtgtgcgc gcaattcaga aagaaaaaac acgcagagca ttagtagaag
     1021 ccgcctttaa tcaattaagc tcagaaaaaa atttctctaa tctcagtttg cgtgaagttg
     1081 cacgtgaagc tggagtcgca ccgacttcgt tttaccgcca ctttaaagat atggacgaac
     1141 taggcttagc aatggtggac gaatccgggc tactattacg ccaattaatg aggcaagctc
     1201 gaaaacgaat tgcaaatggc ggaagtgttg tcgtggtttc agtggaaaca ttcttcgaac
     1261 tgattgccga ccgccctaac gttttccagc tattactacg cgaaagctcc ggtacatcgc
     1321 aagcgtttag aaccgcagca tcacgcgaaa ttcaacattt tgtagctgaa ttggcggact
     1381 atattatgag caaagataat cacagtaacc gagaacttgc ttatattcaa tcggaaggtt
     1441 tagtcacgct agtatttact gccggttctc atgctttgga tatgaacaat caagaacggg
     1501 aaaaactaaa gcaacgtgtc attatgcagc taagaatgct gattcgtggc tccacctatt
     1561 atgccaataa acaagcttca tcaacataaa aaaacagacg cctaagcgtc tgtttttatt
     1621 ctttcgtaat atatgcagtc gatagcttta accattctag tgctaattga tgatgttcag
     1681 ataacgcatt ccataacgca tcttctgaca agatactata attgccttta gtatctaact
     1741 gttcatcaaa actatcatgt aattcacgcc attccgggct agcataaaac cgacttaagg
     1801 gttccaaaag tgcagaagct cgtttccatt gctcaatccc cttttccaat tctggtaata
     1861 gctctaccca ttggttataa caatcctgca ttttctgaat atctgctttt ttcattcttt
     1921 accgcttaca ccataaattt tagcctcaat ataacataaa aatccccttc cgaagaaggg
     1981 gatttcaact caattaggct aaattaccac tggtatccaa cgcctacacc gccaccaaag
     2041 tcgcctcggc tattcgcatt accttgtaat ttcaagataa gcttaccgtt atcacttgaa
     2101 cgtgaataac ctactgcaag cgcgttttca cctttaaatg taccacctgc tgcagccacc
     2161 atagatttac ctggaatgta aacctgcggt aacgctgctg ctgcatttga accggcaata
     2221 ccagcacggg cttcacggtt attacgatgg atcttgttat ttaagtgatt aacagcacct
     2281 tttaactgac caacattaac agcatcggta tctgcctcac ctgctttaac attagtaata
     2341 cggttaccac cattatttaa gccatctttc gttaagctta ccgcttttcc tgcagagcca
     2401 ttattaatgg ttacgccatt attgttcacc aatgtatcac cagtcttgct agtaaattca
     2461 ggggtatctg aaacggctac agttacatca gaacctttac gtgtcacttt gatattatta
     2521 cctgcattca cgctcactgt actacctgct tttactttcg caggggcttt atccccttca
     2581 accgcacctg taccaactgc tttagaatct acgttccagc ttacgttgtt tacggcattt
     2641 gctactgttg ttgcattcac aaagcgtgaa ccggtaccgt taggatcttg aacattgatt
     2701 ttgccgttat cagcaacggt taaatctgtg atgtttacgt tcactttcac ctcaccagcc
     2761 ttatcactgg taatagttgt accattacca tttacgatat tgatagaacc atcttcatca
     2821 gtaccaatgt tattaccatt cactttcgca tagttgttca gctgtttacc gttgataaca
     2881 tctgtgctta ctgggctaat atcgccatct gctacattag tgatcttagt tggtttaccg
     2941 tctttatcac cgactttgat gttatcacca tcattagtga ttttcggacc attctcactg
     3001 aactgtacgc tattgaagct cacatctttt gctgtcgcaa aagtaaattg attaccatca
     3061 cgtttaacag ttaagttatc acctgcttca aacacaactt cttcgcccgt tttcactagc
     3121 ttagtcgcag cttggtcacc ggcagcctta ttacctgcag atttaatatt aaagcctgca
     3181 ttattcactg catctgcaac agtttgagca atcaataacg catttggctg agttggtgct
     3241 ttcaccgtac catcttcgtt gttacctaac tcactggtat ttgcagtgat gttaccttca
     3301 ttatctactt taatagtagt accgtcagtt ttcgcttcaa cgttataagt agttgaacca
     3361 tcagcattcg tgctaggtgt gatagttaca cctcggttac cgcccacttt cgtagtagct
     3421 gcattagttg ccgcttgtaa atcacctaag ttcactacgt tgtttagctt gtcgccttca
     3481 gcatttttaa tcgcttctgc acggtcttta ccctctgggg ttgagccatc ttcgttacga
     3541 acactacttg ccacattgcc taattgaaca ggggcttcac ctgtgaattt agccgtattg
     3601 cttggcgttg agctatcagt aggatcttgg tttacaaact caatcgggct atctacttta
     3661 atcgtattct taccgtcttt gttttcaacc gtgaccgttg tacctttacc gttaataaaa
     3721 tcaaccgtgt cattatgtac cacagtatca accgctttgc cgttaccttg taaattccag
     3781 cctgcattta atacatcacc taatgttgct gcattactta acttatcagc taagttggtt
     3841 ggattattgt ctgctggttg aaccttatca tctgcagtca caggtatcag attagacgca
     3901 acattgctaa tcgctttatt acctgcattg ataccatctt tattgatagt aattggatta
     3961 tttgcaggat ctgcttcatc agttggtaca gttacagtgt tagaggttac gctgctaaac
     4021 ttaacatcat ctttagtcgc ataaatgact ttaccatcag cttcttgctt cacagttaag
     4081 tttttacctg caaccatttc aactgcttta cccggattaa tcagttcatc accacctgaa
     4141 agcttctctc caccttctac agccgacgtt ttcaacgtaa agcctgagtt attgatggcg
     4201 ttggtaatat cacctgctgt cactaatttc ttagcatcat cagcggttgg ttcatcaatt
     4261 ctaccgtcat tatttgcatc atttaatgcc gtagtgttag ccttgatcgc accggttgta
     4321 ggatctttta caattgttac atcgtccgta ttcacagaaa gatcgaacac attcgcgcca
     4381 tcagcatttt ttgtcgtgtt aaccgtaact gatttatcag tcgacttcac ttcttcacga
     4441 gatgccatag cgaaagtata aagctgacta ccatttactg catctttact atctgcattt
     4501 acgtcaccgg cagctacatt agttactttg actgcattac cgtctttgtc accaacattg
     4561 atattaccac cattgttagt aatcttagga ccttcatttc cgaactgtac actatcaaaa
     4621 ttcacatttt ttgcagttga aacagtaatg ttattcccat cacgggtaat ctcaatgttt
     4681 ttaccattta agaactgaac cgtatcacct gttttcacca ggctcttctc gtcaccgttt
     4741 gctttaatgt taaaacctgc attgttaatt gcatctgcaa cggttttagc tgttactaat
     4801 gcatttggtt ctgtaggctc ttccacttta ccatcagggt tatttgttaa ctcgctggtg
     4861 tttgccgtga tttcgccctt atcgtttacc ttaatggttg taccatccgt ttttgcttca
     4921 acggtataag tggttgagcc gtcatcgtta gttttagatg ttacagtaac acctttatca
     4981 ccttccactt tggtggttgc tgctttcatt gctgcttgta catcacccac attcacagca
     5041 ttattcaacg tatcacctgt tgcgttgtta atattatccg caatcgcttt acgctctgca
     5101 tctgttggtg ctgctttatc tgcaaatgta ccacccacgc cgctcttcag attagtgatt
     5161 gagcctgttg tatctggttt accattgctc atatcaatgc tgttgaatgc aacattatct
     5221 ttcgttgtgt aagtgaattt accgtcagct tgggtaagtg tcatattttt acccgcatca
     5281 aaggtaactg tttcacttgg cttgattagc tgtgttttct tacctttggt ttcaccacca
     5341 tcaacaccat cacctgctgt tacattaaag cctgaattat tgattgcatt agcaatatca
     5401 cctgccgtca ctaatttctt agcatcgtca cctgtaggct cagtaacttt accttcagga
     5461 gaattattta atgttgtggt attcgcttta acttcgcctg ttgtcggatc tttcacgata
     5521 gtaacgttgt cagtattcac tgtcatatta ccgtcattat cacgaccgat agatttatcc
     5581 gttgcaacat ctaccgtata ttcagtattg cctttatcat cttcctttcc ggctttaaca
     5641 gttacggctt tagaaccagc tactacagat gaggttttct ctgaattatt aattgcatca
     5701 gccacattac cacgagttac tgtaggagca cctgctttcg ctacatctac cgtatagatt
     5761 gtttgaccat tatcacctgt ttcaggtgca ccaacagttg ctaaacctga acctttcact
     5821 tcggttttgt gacgctgctg cttgttttaa ctgacttaca ttcaccgcat cagtatcagc
     5881 ttcacctgct ttcacgcctg taattttggt tggattacca tcattacctg caatattaac
     5941 attaccatct ttattcgtga ttttcgggcc attatcacca aatgttacgc tatcaaattt
     6001 cacatctttc gctgtttcgt acgtgaatat agtgccaaca cgtttaactt ttaagttatc
     6061 gcctgctgca aaaatcactt tttcgcccgg attaatcaac tctgctgctt cttgatcacc
     6121 atcagcttta ttgccaccag attttgcatt ccaacctgct ttttgaatcg cttcagaaac
     6181 tgtttttgta gttgcaactt tatcttcttc tgcaggtgta gttactgcac ctgttgtgtt
     6241 atcaaccgtg atagaaccgg tatccacatc aaaggttact ttacgccatc agcttcttgt
     6301 gtaacatttg aaactgtacc tttaccgttt acaaattgta cagattcatt tggggtcact
     6361 gtgcctttgg tttcacccgc attatcttta atattgaaac cttggtttac atagttattt
     6421 aagttcgtaa ttgcatcgcc aacgtttgtt gcagtattac catactgagt tccagctgca
     6481 ttcgttacat tatatttagg ctgagtcaca gagccatccg gattcacaac agaaccgcca
     6541 ccaaaattat ttgcagttga atttgcaagg ttactggttg cattcattgc catataaagt
     6601 tgtgaaccat taatcgcatc ggttgatgta gcagaaatct gacccgctgc cacattttgg
     6661 atttgacgct ctttacctgc agagcctaca gaaacaacat agttagtatc tttatttacc
     6721 ccggcaaagc cattataagt aataccccct accgttgcat tttcagttgg tgttgctgcc
     6781 cttacgactg attcgctacc caaagcaaca gaactattca gagtagcttg agctcccata
     6841 cctaatgctg tagcaaattg agctgttgca ttagcacgtg taccaacagc cactgctgat
     6901 tgtgctgagg caattgaatt tgcacctacg gctaaagagg catttgcact cgcatttgct
     6961 gcaacaccaa aggcagaagc tttttcagcc gttgcattag cattagaacc taccgctgta
     7021 gcatcacctg ctgtcgcatt tgtttggcga ccaagtgcgg ttgtaccatt agccgttgca
     7081 tttgctgaat gaccaactgc cgtagcttga ataccttttg aaaccgtagc aacacctaaa
     7141 gataacgctc cgttaccatt tgcatttgag tttgtgccta atgctactgc cgcttgctct
     7201 ccacctgttg ccgttgcatt cgggccgatc gcaattgagt ttctgctctt cgcgccatca
     7261 ttagcttgat tctcaggtac ggttgaattc acagagaaat attgaatttg gcttgcttgc
     7321 gcaggaatat tgacagaaag atcataaata accgtgccat ttgcatttct ctctgtactt
     7381 tttacagtta ctgtaccatc gtttgaaaca acagtagctt gggtatcttt cgtaaagtca
     7441 tcaatggctt tcttagttaa accaatttca tagttagttg tattcgcagc agttgcagct
     7501 ttacctgtta cagtaatgta atcagggcta gaagaatttg ccgtggtgtt atacgcattt
     7561 actgtataaa tagtttgccc atttgcaccg gttgcatttg taatattaac attgctacct
     7621 gcaacaactt ctgtttttac cgcttttaat gcttcttcaa tcgtattttt atttgttcca
     7681 ccaatattag tataggtaac agaaccatca ggctttactg tagcattacc acctaaaata
     7741 tttttagttg aattagcaag gttacctgtt gcgttcattg ccatataaag ctgtgaacta
     7801 ttaattgcat cggttgatgt agcagaaatc tgacccgctg ccacattttg gatttggcgt
     7861 ttgatcgtgt ttgagccaac agaaacaaca ttccctgctg ccgtagtatt agccgcaaag
     7921 ccggaataag taataccatt taccgtcgca gaaggaactg ccacagcgtt ggaaacggtt
     7981 gaattactcc ctaaagcaac cgaattattt agcgctacat tagcacctga gcctaaggca
     8041 atagcaaaac cggcacttgc tttagcacta tcaccaattg caaccgtaga ttggttggaa
     8101 gatactgcac ttacccctaa agctgtcgca ttaacacctg aaactgtcgc attataacca
     8161 atcgctgtac ctctagttgc agtagaagtt gcattagcat tagcacttga acctaaagct
     8221 aaagaataag tagcatttgc aacagactta tcccccattg cacctgaacc cattgcaaat
     8281 gaacgagtgc cactagcggc agatctccat cccattactg ttgaacggtt accagtagct
     8341 agagctcctg caccaaaagc tgttgcacgg ttacctgttg cagcagtatc cgtacctaat
     8401 gcaatagcat ttgctgaagt cgctctagcc cccataccaa tagcaataga aggaactaat
     8461 gcccaattct gaccagtgtt attgagactc tctacaccaa tattatagta atgtaagaag
     8521 tatcttctgg atccactaaa tttgttctat gcgttgcatt cgtatctgta ccaattgcaa
     8581 tagtagatct gccagttgag attgaatccg caccaatagc aattgcgttc tccgatgatg
     8641 cagttgcttg tgttgaaata taattacttg ttgttgaacc tataagatac aacagccaca
     8701 gcatcattag ctcccaacac ggcactagct ccagccaata aagccaatgt tgccttactt
     8761 gcagaactaa cagatttcgt tttacctttc gctcgttgta attcagatac tgcaacccaa
     8821 ctttgtgttg cctgattcca aataacttta aaaatttgat tcataatcga ccctatataa
     8881 aaataaaaaa gattacgtag aacctatgtt caacataatc ttcaccaata taaaaaaccg
     8941 ccacctattt aagtgaaata acttacttat agactcagaa atttaagtgc gcgtatttta
//
LOCUS       DANLZC010000102         8895 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.102, whole genome shotgun sequence.
ACCESSION   DANLZC010000102 DANLZC010000000
VERSION     DANLZC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.102"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..344)
                     /locus_tag="PXE44_001857"
     CDS             complement(<1..344)
                     /locus_tag="PXE44_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589342.1"
                     /translation="MPFKTVTFAQATTNDGQSLLAVNDLFIGPKSHTSAQYILQWNGA
                     EEVQSSSGIIVSTGLGSTGWFQSILAGAMAITGEASHPLLQGFSWSDRKLQFSVREPF
                     PSRTTGVALTFGT"
     gene            complement(393..572)
                     /locus_tag="PXE44_001858"
     CDS             complement(393..572)
                     /locus_tag="PXE44_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589343.1"
                     /translation="MFLVNQLLAITIATSAVLTNAQLAILAVAFWIAILPCARLATAV
                     GVWVFDDVVHNNSPN"
     gene            661..816
                     /locus_tag="PXE44_001859"
     CDS             661..816
                     /locus_tag="PXE44_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL5589344.1"
                     /translation="MGLNRAEMMYREGAYVIDPVFPATLGYAGAGVVVAVGDDAGEFQ
                     IGDKVSV"
     gene            complement(906..2453)
                     /locus_tag="PXE44_001860"
     CDS             complement(906..2453)
                     /locus_tag="PXE44_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HDL5589345.1"
                     /translation="MKRRDFLRYGIGISLASSSLYSLANMMNHAQHGNMAMMHSVPTG
                     LMPTEAMPSGLSLNGILPKLANQSTQAGLFKATLKAEPIKIRLADNKETEFWAYNGQL
                     PGPQIEVFEGDTVEIEFINHLPQPSTVHWHGLDVPNEADGNPMDMVEPQGKKVYRFTL
                     PQGSAGTYWYHPHPHDHVSEQVYKGLAGTFVVKAKNDPLAHLPEQHWVISDLRLNADG
                     TIPANTMLDWINGREGEFVLINGQYQPQIQVKTNERIRLWNATSARYFRLNIPGVKWI
                     VVGTEGGLLEKPRSPVDELLLTPAERVEVIMVGETQGTVNLQSGYYDRRKMMVQEQPK
                     DLTLATIQVKSEPVQLPESLRSLPNWDEPKQVRQIRFSEKMMNHTNMPMMNHGTHHAT
                     TTLTDNPIPPMMNGMFLINGQTFDMNRIDFVTKLNEVEQWEIFNESHMDHPFHLHGTQ
                     FEVIQHTLNGKTFKPEGRALKDVVNLRPYEKVIIRFKQGHTGLKMYHCHILEHENLGM
                     MGMFKVE"
     gene            complement(2467..2922)
                     /locus_tag="PXE44_001861"
     CDS             complement(2467..2922)
                     /locus_tag="PXE44_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HDL5589346.1"
                     /translation="MKLHKFTRSLLLLSLGITAFAQAQTLSMEVWKSPTCGCCNEWIS
                     YMQKNGFEVKVNETGNYDAHKRFNIKYEHASCHTAVIDGYVIEGHVPAEDIKRLLAEK
                     PDAIGLSAPGMPIGSPGMDGEAYGGRKDPYDVVLIKKNGESEVFKSYNQ"
     gene            complement(3104..3424)
                     /locus_tag="PXE44_001862"
     CDS             complement(3104..3424)
                     /locus_tag="PXE44_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589347.1"
                     /translation="MELKVIHKNLVDSAKIKLHAVYLVIFLFVSHFAMIFGMADPISA
                     GYQPTTPAMTHSMQMDAKQITEMHNQMMTGEITPEEMMKMHQEMNPNMNMPNTQGGHS
                     EHHK"
     gene            3423..4190
                     /locus_tag="PXE44_001863"
     CDS             3423..4190
                     /locus_tag="PXE44_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL5589348.1"
                     /translation="MSKIIKIGKVANNSDAIDAISTQPRHSVIVGTIKRLQTDRIDLL
                     YQHRVDPNVPIEEVAGAIKDLMKQGKVLNWGLSEAGEKTIRKAHAIQPLTAVQSEYSL
                     FFRGREKDVILVCEELGIGFVPWSPLAMGALAGYVDENSRFEADPSKDLRGIIPRFTP
                     EAMQQNVKLIQLVQKWAKRKLCTPAQLSLAWLMAQKAFIAPIPGTSKARHLLDNLGAD
                     DVKFTEEELAQFRQELEAIEIVGLRLPEAILNFSEAK"
     gene            complement(4729..5439)
                     /locus_tag="PXE44_001864"
     CDS             complement(4729..5439)
                     /locus_tag="PXE44_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589349.1"
                     /translation="MWKKIRIDFTDTVKCQFCSRMITSGKAIVVQNELGNISFSGPSC
                     AQNKNGQNVVNPKEKIVDITKGCLLHEPTTNDKTDKQVDVEQDNFTSIHDDEVHNYLD
                     NNAVNAYLTLRFEKLSHLTSIIKSQKLNEIYHNYELTGQISQKDESYIRVIMYGEAYP
                     LYTYQNLQAVYAAEYWLQSFIQHNPENDLNFIQDTLNQLRRKLTLTEKQIMGINNWFK
                     NSDGKMVKLKTNAFVRGK"
     gene            5916..6434
                     /locus_tag="PXE44_001865"
     CDS             5916..6434
                     /locus_tag="PXE44_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5589350.1"
                     /translation="MRYIKYSLFLVSTMLISGCAELSAINDKVGDWAGQMNQTLYGSK
                     DGSVEQHNGRQVYTKTRNGKYVYQDSGTLQLKRNIDDVFLKVRREFGFRPTNADSDSR
                     KVYHTVQGTFYHVSGYFESDVDTDGVHLGENYLEVIIEKGSKNQVNVEWKATGSARWL
                     DSAKQRLLNTLK"
     gene            6594..8645
                     /locus_tag="PXE44_001866"
     CDS             6594..8645
                     /locus_tag="PXE44_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL5589351.1"
                     /translation="MKLFICEKPSQARDLAGVLGATSKGEGLLATADKSIIVTWGFGH
                     LVEQYQPEDYDEAWKRWAFETLPIIPAQWKMTPKKESKKQYSVVIGLIKKATSVVIAT
                     DADREGEMIARELLDLAGYRGQIQRCWLSALDDASIRKALQTLKSGKETEALYYAGMG
                     RSRSDWLIGMNFSRLFTLMAQAKGYSGKPLSVGRVQSPTLTLVVNRDREIANFVPKSH
                     YALAVQLTTTSGETFVAGLAVPEQYLDESGLCLDSRVIQQAEAQIKQTGLAKVVTVET
                     KREKKSPPLLYALSDLQGECNRLFGFGAQQVLDIAQSLYEEHKITTYPRTDCGYLPES
                     QFSDVPNVIRSLVASDSGLQTLLPKLNLRQKSRAWDDKKITAHHGIIPTIKKADLSKL
                     SEEEMKVYDLIRRRYLAQFLPHLEMDKTVATLQSSGHTLVARGNVIVAQGWRILFGKN
                     VDEETGNDDEKQGLPVLTKNQQCNVTHSEVRTLQTKPPAHYTEGTLLDAMKNAARFVT
                     DPRLKQRLRETEGLGTEATRAGLIQGLIDKGFLLKKKKSLVASAEAVALIDSLPDLLK
                     NPGLTALWEQALNQIAEGSMTLDAFMQKQEAFVKQLIGSCMQQGMSLGNIEIRKCSEC
                     GKPMRKINHAKGTFWGCTGYPDCQHKEADKKVKSASNKSAKKSSNLNVLDQLNKLRSQ
                     L"
     gene            complement(8706..8843)
                     /locus_tag="PXE44_001867"
     CDS             complement(8706..8843)
                     /locus_tag="PXE44_001867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589352.1"
                     /translation="MTLQSRFRQLQQTNVAGIIHLMFSWHSHKVEFPLRMLGDILAGV
                     D"
BASE COUNT         2673 a         1724 c         1782 g         2716 t
ORIGIN      
        1 gtgccaaaag tcagtgcaac acctgttgtt ctacttggaa atggctctct tacactaaat
       61 tgtagctttc gatcactcca gctaaagcct tgtaatagag ggtgcgaagc ttctcctgta
      121 attgccatcg caccagcaag aatagattga aaccaccccg ttgatcccaa tcctgttgat
      181 acaataatgc ctgatgaaga ttgcacttct tcagcgccat tccattgcaa aatatactgt
      241 gcggaagtgt ggcttttagg gccaataaat aagtcattaa ccgctaataa ggattgacca
      301 tcattggttg ttgcttgtgc aaaagtgacc gttttaaatg gcattttttt attaatgggt
      361 ttaaaaacgg tttttgtttt gataatcaac ctttaatttg gagaattgtt atgaacgaca
      421 tcatcaaaaa cccaaacgcc cactgcggtt gccaatcttg cacagggcaa aattgcaatc
      481 caaaatgcaa ctgccaaaat ggcgagctgt gcatttgtca aaactgcact tgtggcgatt
      541 gtgattgcaa gtaattgatt aactaaaaac attgggcggg cttaggtttg cccaatgttt
      601 gccaaatccc attttgataa ggaaaactat aatgaaaatg tttaaaaacc gtgccgtgca
      661 ttgggactaa accgtgccga gatgatgtat cgggaagggg cgtatgtgat tgaccctgtg
      721 ttccctgcga ctttgggtta tgcaggggca ggcgtggtcg tggcagtggg cgatgacgct
      781 ggcgaatttc aaatcggcga caaagtgagc gtctgatctg caccccaaaa gttggactaa
      841 aaaccaactg attaaggtgc agatttttta tgacgaaatc cctctatttt tatagttaga
      901 taccactatt ccactttaaa catccccatc ataccgagat tttcatgttc taaaatatgg
      961 caatggtaca ttttcaaccc agtatgtcct tgcttaaagc gaataatcac tttttcataa
     1021 gggcgtaaat taaccacatc ttttaacgct ctgccttctg gcttaaaggt tttaccgttt
     1081 aacgtatgtt gaattacttc aaattgagtg ccgtgtaaat ggaatggatg atccatatgg
     1141 ctttcattga agatttccca ctgttccact tcattcaatt tggtaacaaa atcaatacgg
     1201 ttcatatcaa aggtttgacc gttgattaag aacataccat tcatcattgg tggaataggg
     1261 ttgtccgtta gtgttgtagt agcgtgatgt gtaccgtgat tcatcattgg catattcgta
     1321 tggttcatca ttttttcact aaagcgaatt tggcgaacct gctttggctc atcccagtta
     1381 ggcaaggacc ttaagctttc aggtaactga acaggttctg atttcacctg aattgtggct
     1441 aaagtgagat ctttaggctg ttcttgaacc atcatcttac ggcgatcata ataaccgctt
     1501 tgtaaattga ctgttccttg cgtttcaccc accataatca cttcaacgcg ttcggctggt
     1561 gtaagtagca gttcatcaac aggcgagcga ggtttttcaa gtaaaccacc ttcggtaccc
     1621 accacaatcc atttcacccc tggaatgttt aaacgaaaat aacgagcgct agtggcattc
     1681 caaagacgga ttcgttcatt cgttttcact tgaatctggg gctgatattg accgttgatt
     1741 aacacaaatt caccctcacg accgtttatc caatctaaca tcgtgtttgc tggaatggtg
     1801 ccatcggcat ttaaacgcag atcggaaatc acccaatgtt gttcaggaag gtgtgcaagc
     1861 ggatcatttt tcgctttaac aacgaaagtg cctgctaagc ctttatagac ttgttcggaa
     1921 acatggtcat gtggatgagg gtggtaccaa tatgtcccag cactgccttg aggtagcgta
     1981 aagcgataga cttttttccc ttgcggttca accatatcca tcgggttacc atccgcctca
     2041 tttggtacat ctaagccgtg ccaatgtact gttgagggtt gtggtaggtg attgataaat
     2101 tcaatttcta cggtatctcc ttcaaatact tcaatttgtg gccctggtag ttgtccgtta
     2161 taagcccaaa attccgtttc tttattgtct gcgaggcgaa ttttaattgg ctctgctttt
     2221 aaagtggctt tgaacagtcc agcttgagtc gattgattag caagttttgg taatatccca
     2281 ttaagcgata aacctgaagg cattgcctca gttggcatta ggccagtagg tactgaatgc
     2341 atcatcgcca tattgccatg ttgtgcgtgg ttcatcatat tcgcgaggct ataaagcgag
     2401 ctactggcta agctaattcc aattccataa cgtaaaaaat ctcgacgttt cattctttcc
     2461 ccccccctat tggttatagg atttaaacac ttcactttca ccgtttttct taatcaaaac
     2521 aacatcataa ggatctttgc gtccgccgta tgcttcaccg tccatacctg gtgaaccaat
     2581 tggcattccc ggtgcagaca agcctattgc atcgggtttt tctgcgagta aacgtttgat
     2641 atcctcagca ggtacatgtc cttcaatcac atagccatca atcacggctg tgtggcaaga
     2701 agcatgttca tatttaatgt taaagcgctt atgagcatca taattgcctg tttcgttgac
     2761 tttaacctca aaaccgtttt tctgcatata gctgatccat tcattacaac agccacaagt
     2821 cgggcttttc cacacttcca tagaaagtgt ttgtgcttga gcaaaagcgg ttatgcctaa
     2881 acttaaaagc aacaatgagc gggtgaattt atgtaatttc ataagatgtc cttatttttt
     2941 attgagattg aatggacatc attttaaagc ttaacctagg gttaaggtca agtaatctga
     3001 aaaaattcta tcatttgaaa atttttctca aaatcctact gcttgtagta gagatatgcc
     3061 aacgtaccat ccactattat tttttaaaat gtgatcagaa ttattattta tgatgttctg
     3121 aatgaccacc ttgagtattc ggcatattca tattagggtt catttcttga tgcatcttca
     3181 tcatttcttc aggcgttatt tcgcccgtca tcatttggtt gtgcatttct gttatttgtt
     3241 tagcatccat ttgcattgaa tgagtcattg ctggtgtagt gggttgatag cctgctgaaa
     3301 taggatctgc catgccaaag atcatagcaa agtgactcac aaataagaaa atcacaagat
     3361 aaaccgcatg taatttaatt tttgcactat ccacgagatt tttatgaata acttttaatt
     3421 ccatgagtaa aataataaaa ataggaaagg tagctaataa tagtgatgca attgatgcga
     3481 tatctaccca accacgccat tcagtaatag tcggtacgat aaaacgctta caaaccgacc
     3541 gtattgactt actctatcaa caccgtgttg atcccaatgt gccgattgaa gaggtggctg
     3601 gcgctattaa agatttaatg aaacaaggca aagttttgaa ttggggcttg tcggaagcag
     3661 gcgagaaaac cattcgcaag gcacacgcta ttcagccttt aaccgccgta caaagcgaat
     3721 actcgttgtt tttccgtggt cgtgaaaaag atgtcattct cgtttgtgaa gaattaggga
     3781 ttgggtttgt gccttggtcg ccgctggcaa tgggagcatt ggctggttat gtggacgaga
     3841 acagccgttt tgaggctgat ccaagcaaag atttgcgtgg tattatccca cgctttacac
     3901 ccgaagcgat gcaacaaaat gtgaagttaa tccagcttgt gcaaaaatgg gcaaaacgca
     3961 aactttgtac gcctgcacag ctttctttgg cctggctaat ggcacagaaa gcgtttatcg
     4021 cgccaattcc tgggacgagc aaagcccgtc atttgttgga taatttaggc gcagacgatg
     4081 tgaaatttac cgaagaagaa ttggcgcaat tccgccaaga attagaagcc attgaaattg
     4141 tcggcttgcg tttgcctgaa gcaattttga atttttcaga agcgaaatag ttttcagata
     4201 cctgaaaaag tcgtaaataa aaattctttc ttacagcggt cgttttcata aaatcctttg
     4261 aaaaacgatc gcactttttc ttgaatatgt agcaagatta gtggaagatt tatcgaaatt
     4321 gaaagatatt gggaaaaggg taaaactgga atcagacaga tgattaggca aaacgctata
     4381 gtcaataagt gtaaaaatgg cataatcaac tcaatgaatt tatggacaaa tattatctgt
     4441 ttggtatttt atagtaagta cattgtgtaa aaataaaatc tgaaatttag tctttttata
     4501 ctttattttt acgtactcat acttcaatct gggaaactta atatttaatt aattgattta
     4561 aaagaatttt ataatttaca ttcctaagtg atatagaaac aaccgttttg aattacatat
     4621 aacttgaatt tgtcaaaata agcaagcaaa taataggaaa gagtctttta aaaccagtct
     4681 tacgtcgggc ctacgtaaca aaataagtgg ccaccacaat tatgttaact atttccctct
     4741 tacaaaagca tttgtcttta atttcaccat ttttccatca gaattcttaa accaattatt
     4801 tatgcccata atctgttttt cagttaatgt tagctttctt ctgagctgat ttaatgtgtc
     4861 ttgtataaaa tttaagtcat tctctggatt atgctgaata aatgattgaa gccaatattc
     4921 agcagcataa acagcttgta agttttggta tgtatacaaa ggatatgcct caccatacat
     4981 aatcactctt atgtaacttt catctttttg agatatttgc cctgtaagtt catagttatg
     5041 atagatttca tttaactttt gagattttat aatcgaggtt aaatgcgata gtttttcaaa
     5101 tcttaaggtc aggtatgcat taacagcatt attgtccaaa taattatgta cttcgtcatc
     5161 gtgtatggat gtgaaattat cttgctcaac atctacttgc ttatccgttt tatcatttgt
     5221 tgttggctca tgaagtaaac atccctttgt gatatcaact attttctctt taggattgac
     5281 tacattctga ccgttcttat tttgagcaca agacggtcct gaaaaagaga tattgccaag
     5341 ttcattttga acaacaatag ctttgcctga tgtaatcatt ctactgcaaa attgacattt
     5401 cactgtatca gtaaaatcaa tcctaatttt tttccacata acatatctct ccatatagtg
     5461 agtaataaaa atacttctta actctcttca ttgatgaaat aattgcaaaa ataactttga
     5521 tagaggataa atatatgaat ttccctccaa ctcgatggaa aatattacac cagtatcaaa
     5581 ctgagctaga gctctgggtt gaacaagcca cagaagagca tatcaagcaa tacttgactg
     5641 aaattcacgg atgtgtatac ccagatgact gggaaaatgt gcgtatatcc catatgtcat
     5701 ctcaaactca tattttccat agctgtgaac gttgcctaga acttatctct gcgatattaa
     5761 caatctcaca actaagcaga cccgatctaa atggtctact gtgtgacaga aagctataat
     5821 attttttctc tttgtaagta aaagagtttt aagtagattt taaccgcact tttgcaaaaa
     5881 atgaacgcag ataacccgct tgtaaggaga attctatgcg ttacatcaaa tatagtttat
     5941 ttcttgtttc tacaatgcta atttcaggct gtgctgaact ctcagcaatt aacgataagg
     6001 ttggggattg ggccgggcaa atgaatcaga cgctctatgg ctctaaagac ggctctgttg
     6061 aacagcataa tgggcgacaa gtttatacta aaacccgaaa tggtaagtac gtttatcaag
     6121 attccggcac attgcagttg aagcggaata ttgatgatgt attcctgaaa gttcgtcggg
     6181 aatttggctt ccgcccaacg aatgctgata gtgactcccg taaagtttat catacggtac
     6241 aaggtacttt ttaccatgtt tccggctatt ttgaatctga tgtagatact gatggggtac
     6301 acttaggtga aaactacttg gaagttatca ttgaaaaagg cagtaaaaat caggtgaatg
     6361 ttgaatggaa agcaactggt agtgctagat ggctcgatag tgcaaaacag cgtttactta
     6421 atacattaaa ataataactc atattgctcc tagaacctct tcccagtgga aacactggga
     6481 ttttttatgt ccttatttct caaattatta catcctacga gacaataagt cgttgaatgc
     6541 tatttaatca gcacattact atttgcctat ctgtattcat tttaggaaat aatatgaaat
     6601 tatttatttg tgaaaagcca tctcaagctc gagatcttgc cggcgtattg ggtgctactt
     6661 caaaaggtga aggcttgctg gcgactgcgg ataaatctat tattgtcact tgggggttcg
     6721 ggcatctcgt tgagcaatat cagccggaag attatgatga agcgtggaaa cgctgggcat
     6781 ttgaaacatt acccattatt cctgctcagt ggaaaatgac accgaaaaaa gagtctaaga
     6841 agcaatattc tgttgtaata gggcttatca aaaaagcaac ctccgtggtc attgcgaccg
     6901 atgctgatcg agaaggtgag atgattgctc gtgagctgtt agatttagct ggataccgag
     6961 gtcaaatcca acgttgctgg ctatcagctc tggatgatgc cagtattcga aaggctcttc
     7021 aaacactcaa atcaggtaaa gaaaccgagg ctctttacta cgccggtatg ggacgtagcc
     7081 gctcggactg gttgattgga atgaacttct cccgtttatt taccttaatg gctcaagcaa
     7141 aaggctattc cggtaagcca ttgagcgttg gtcgagtaca aagtccaaca ttaactcttg
     7201 tggttaatcg agatagagaa attgctaatt tcgtgcctaa atctcattat gcgttagcgg
     7261 ttcagctgac aacaacctct ggtgaaactt tcgttgccgg gcttgcagta cctgaacagt
     7321 atttggatga gtccggactt tgccttgata gtcgtgtcat acaacaagcc gaagctcaaa
     7381 ttaaacaaac aggcttagcc aaagtggtga cggttgaaac taaacgagag aaaaaatctc
     7441 caccgttact ctacgcatta agtgatttac aaggcgaatg taaccgcttg tttggctttg
     7501 gggctcagca ggtgttggat atcgcacaat ccctttacga agagcataaa atcacgactt
     7561 atccccgtac agactgtggt tatctgccag aatcacaatt ttctgatgtt cctaacgtaa
     7621 tccgctcatt agttgcctct gattcagggc tacaaactct cttgcctaaa ttaaatttgc
     7681 gacaaaaaag tcgggcgtgg gacgataaaa aaattaccgc tcaccacggt attatcccaa
     7741 ccattaaaaa agctgattta agtaaattga gtgaggagga aatgaaggtc tatgatctca
     7801 ttcgccgccg atatcttgct caattcttac cgcacttgga aatggataaa accgttgcca
     7861 ctctgcaatc tagtgggcac actctggttg ctcggggaaa tgtcatcgtt gctcaaggct
     7921 ggcgtatctt gtttggcaaa aatgtggatg aggaaactgg taatgatgat gagaaacaag
     7981 gattacctgt attgactaaa aatcagcaat gcaatgtcac tcattctgaa gtccgaacat
     8041 tgcaaactaa acctcccgca cactatacag aggggacatt actcgatgca atgaaaaatg
     8101 ccgctcgctt tgtgactgac ccaagactca aacaacgctt acgtgaaacg gaaggtttag
     8161 gcacagaagc aacccgtgcc ggattaattc aaggcctaat tgacaaagga tttctcctta
     8221 agaagaaaaa atcactggtt gcctcagcag aagccgttgc cttaattgat agtttgccgg
     8281 acttactgaa aaatcccggt ttaacggcat tatgggaaca agccctcaat cagattgccg
     8341 aaggtagtat gacacttgat gcatttatgc aaaagcaaga agcatttgta aaacaactta
     8401 tcggcagttg tatgcaacag ggaatgtcac ttgggaatat tgaaatccga aaatgttcag
     8461 aatgtggtaa gccaatgaga aaaatcaatc acgctaaggg aacattctgg ggatgtactg
     8521 gttatccaga ttgccaacat aaagaggctg ataaaaaggt aaaatcagct tcaaataaat
     8581 ctgctaagaa aagtagtaat ttaaatgtac tcgatcaact gaataaatta agaagccagt
     8641 tataaattca agtagattat ggtattatca aggaacaaag aacccgtttt gtagattgtc
     8701 ggttatcaat ctacaccagc caaaatgtct cccagcattc tgagagggaa ctccactttg
     8761 tgggaatgcc aactaaacat taaatggata atgccagcca cattagtctg ttgtaattgt
     8821 ctaaatcgtg attgcaacgt cataatatta cccgattggg gggattatga cgttgcaatt
     8881 actgctaact attct
//
LOCUS       DANLZC010000103         8845 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.103, whole genome shotgun sequence.
ACCESSION   DANLZC010000103 DANLZC010000000
VERSION     DANLZC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.103"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            8..1321
                     /gene="pilQ"
                     /locus_tag="PXE44_001868"
     CDS             8..1321
                     /gene="pilQ"
                     /locus_tag="PXE44_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HDL5589353.1"
                     /translation="MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNM
                     VLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQEDDIVYIHKKEEKAADLTPLPI
                     MNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
                     SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGH
                     YKFGGRLEGNGLNNVTNNLNVNFPITGGASAVLQVSSINSRVLDLELSALEQENSVEI
                     IASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLGLEVVPHLSTQNQIL
                     LDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPI
                     LGSIPFIKRLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKKSK"
     gene            1402..1815
                     /gene="nusB"
                     /locus_tag="PXE44_001869"
     CDS             1402..1815
                     /gene="nusB"
                     /locus_tag="PXE44_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL5589354.1"
                     /translation="MKISPRRRARECAVQAIYSWYISQNTVEEVELAFVTDQDMKGVD
                     MPYFRKLFRGVVDNVEAIDEALRPYLDRKESDVDPIERSILRLSTYELKFEHDVPFRV
                     VINEGIEVAKVFGSDDSHKYINGILDKLAPALGRK"
     gene            1878..2858
                     /gene="thiL"
                     /locus_tag="PXE44_001870"
     CDS             1878..2858
                     /gene="thiL"
                     /locus_tag="PXE44_001870"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDL5589355.1"
                     /translation="MGEFDIIERYFNASQRPPRKDVLLSIGDDCALTSLKPNQQLAIT
                     TDTLVCGTHFLPTISPADLAYKSVASNLSDLAAMGAEPAWISLALTLPEINHDWLAEF
                     SEHFFEILDHYNVDLIGGDTTCGPLSLTITAQGIVPQDRGLFRHNAKVGDWIYVSGTL
                     GDSAGGLQLLLNQQSEQVNWNKEQSYLIQRHLRPTPRVLLGLTIASIANAAIDISDGL
                     LSDLSHILKRSDCGAVLNLENFPLSDVLLSCYSRTEAEYFALNGGEDYELCFTVPNDN
                     KAKLDKALANIGVTYTCIGQIRSAKEGLTLLRHNQPVTLPSQRGYDHFKK"
     gene            2863..3336
                     /locus_tag="PXE44_001871"
     CDS             2863..3336
                     /locus_tag="PXE44_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL5589356.1"
                     /translation="MKPINLKNPIHFFAFGFGSGLLKPAPGTWGSLAGLIVAIALWSI
                     TQSALFFGLLTLVSFIAGCVICTKASQDLEVHDDGRIVWDEIVAIFLMFTCLPEYNLL
                     GYLLAFISFRIFDILKPFPIRYFDEKLESGLGIMFDDILAAIFALISLHLLYYFI"
     gene            3343..3966
                     /locus_tag="PXE44_001872"
     CDS             3343..3966
                     /locus_tag="PXE44_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDL5589357.1"
                     /translation="MLTILFIHFAGLISPGPDFFYVVRQSASHSVKSGVLAATGISIG
                     IIFWAAFAIFGLAWLSNSIGSLFQYAIMIIGGCYLVYIGSQMVRVTENIIFVENKAQL
                     TPLNPWQEIKKGLLINIFNAKAGVYFTSVISAYVDNLSNTLQMFELLLLFVVSTFIYF
                     CVVALLFSRRPVRLFYAKYSRYIDNISGVIFILFGSMLIYEGFSHFI"
     gene            4078..4683
                     /gene="ruvA"
                     /locus_tag="PXE44_001873"
     CDS             4078..4683
                     /gene="ruvA"
                     /locus_tag="PXE44_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005686751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL5589358.1"
                     /translation="MIGRLHGKIIEKQPPEILLDVQGVGYELLLPMTSFYSLPQIGEE
                     ATIFTHLVVREDAHLLFGFAQKQDRTLFRELIKTNGVGPKLALAILSAMSVSQFATAI
                     ENEELAKLTKIPGIGRKTAERLLVELKGKFKGMAQSDFFVEVSHEQIVASNTPDPANE
                     ALDALIALGYKPADAEKMIKKVNKSGATSEQLIREALKNSL"
     gene            4820..5824
                     /gene="ruvB"
                     /locus_tag="PXE44_001874"
     CDS             4820..5824
                     /gene="ruvB"
                     /locus_tag="PXE44_001874"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL5589359.1"
                     /translation="MIEADRIISASPKREEEVIDRAIRPKLLADYVGQPSVHDQMEIF
                     IKAAKLREEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLT
                     NLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTLIVKRSADCLNLNLSADGAYEVARRSRG
                     TPRIANRLLRRVRDYADVRNNGIITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERF
                     DGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGITQID
                     "
     gene            complement(5923..6606)
                     /gene="artM"
                     /locus_tag="PXE44_001875"
     CDS             complement(5923..6606)
                     /gene="artM"
                     /locus_tag="PXE44_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDL5589360.1"
                     /translation="MWWDYLVEISQGIGTSLQLTFVALIIAFILAIILTFILSLENRP
                     LKWLVNGFITLFTGTPLLIQFFLIYSGPGQFEWITESAAWVLLSDAWFCAMLALALNS
                     AAYSTLLFHGAVKAIPKGQWETCAALGLSRLDTLKILVPYALKRALPSYSNEIVLVFK
                     GTSLASTITIMDIMGYARQLYGTEYDALSIYGMAGVIYLVITGIMTIVLRKLESRVLA
                     FERFETEKA"
     gene            complement(6606..7277)
                     /gene="artQ"
                     /locus_tag="PXE44_001876"
     CDS             complement(6606..7277)
                     /gene="artQ"
                     /locus_tag="PXE44_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDL5589361.1"
                     /translation="MSSDILNLLVNATLMTLGIALASLLIGMLLSIFFVVLETSKIAC
                     IRKPTSIIITLLRGLPEILVVFLIYFGLIQVIFLITDEFAELNAFWCGAIALGIIFAA
                     YASQSLRGAIKAIPVGQWESGTALGLSRAQVFVRIILPQVWRHALPGLSNQWLVLLKD
                     TALVSLIGVDDLMRQSELINANTHQPFTWYGIAALIYLAITLISQRVIKVLEGRFTRF
                     ERGVA"
     gene            complement(7277..8002)
                     /locus_tag="PXE44_001877"
     CDS             complement(7277..8002)
                     /locus_tag="PXE44_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL5589362.1"
                     /translation="MKKLLLASAIAFATLSANAQETITFAMEPSYAPFETTNEKGEVI
                     GFDVDIAKALCEELKATCEFKTQSFDSLIAGLKFKNFDAIISGMDITEARQKQVAFTN
                     AYLENSVSYVAMKGKFTPETAKNVGVQNGTTFQQYLSKNAKQYNLKPYASLQSAVLDL
                     KNGRVDMVFGDTPVLAEWLKTEKELDFVGEKVVDKAFFGNGYGIAVNKSNTALLEKLN
                     SALATIKTNGKYEQIYNTWMAGK"
     gene            complement(8028..8762)
                     /gene="artP"
                     /locus_tag="PXE44_001878"
     CDS             complement(8028..8762)
                     /gene="artP"
                     /locus_tag="PXE44_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDL5589363.1"
                     /translation="MAIRIQNVNFFYGSNQALFDINLEIEKGDTVVLLGPSGAGKSTL
                     IRTLNLMEVPQSGTLAIANHQFDLTAKTDSKAVSLLRREVGMVFQQYHLWPHLTVMQN
                     LIEAPMKVLGLSKEEAVARAKGHLARLRLSEFAERFPLHLSGGQQQRVAIARALMMQP
                     QVLLFDEPTAALDPEITAQVVDIIKELQSTGITQVIVTHEVGVARKVASKVVYMEKGT
                     IIEQGDASCFESPKTEQFAQYLSHSE"
BASE COUNT         2660 a         1879 c         1708 g         2598 t
ORIGIN      
        1 ggttgatatg tggcgaattt ttatatattt cttatgcggt ttttcattag cctatgcgaa
       61 taccatttcc atagttttaa aaaatgctcc aacttcgctt atttttactt atttagcgga
      121 agaaaccggt aaaaatatgg tgcttgatga taatattgaa accaaatcaa cactacgatt
      181 agaaaataaa tcggttgacg agatttttaa aacgcttggc aaagtcaata aattatcctt
      241 aacgcaagaa gatgatattg tttatattca caaaaaagag gaaaaagcgg ctgacttaac
      301 gcctctgcca ataatgaatt tgcaaaataa tgggcaaaat acaccgcttg tcaccgcccc
      361 taagttaatc acaaaaacct tgaaactaca ttatgcaaag gcttcagagg tgattgagtc
      421 gttaaccaaa ggcagcggaa cgtttttatc ggaaaacggt tatattcact ttgatgaaag
      481 aagcaatagc ctgattgtga aagacagtgc aaaatcgctt aaaaatatcg agaatttagt
      541 aaaacagctc gaccaaccca ccgagcaaat tgcgattgag gctagaattg tcactatcag
      601 tagcgagcat ttgcaagagc ttggcgttcg ctggggaatt ttttctcgtg gggctgggca
      661 ctataaattt ggcggacggc tggaaggcaa tggactcaac aatgtcacca ataacttaaa
      721 tgtaaatttt ccgattactg gtggagcatc tgccgtatta caggtttcgt ccattaatag
      781 cagagtgctt gatttagaat taagtgcatt agaacaagaa aatagtgtgg aaattattgc
      841 cagcccacgc ctgttaacca ccaataaaaa gccggcaagt atcaagcaag gcacggaaat
      901 tccttatgtg atgtacaaca ccaaatcgga agccaccgat gtcgaattta aagaagcggt
      961 attagggctt gaagtcgttc cacacctttc aacccaaaat caaattctac tcgatctgat
     1021 cgttacccaa aattcgccaa attcccaatc cggcagtagt ggcttaatta cgattgataa
     1081 acaagagcta aatacgcaag tatttgccaa acacggcgaa accattgtac ttggtggtat
     1141 ttttcaacat ttaacccaaa aaggcgaaga taaagtgccg attttaggct caattccctt
     1201 catcaagcgg ttatttagtc aaactcgaga taaaatcagc aaaagagagc tagtcatttt
     1261 tgtgacgcct tatatccttc aatctaatga aaagagcagc aaccgcaaaa aatcaaaata
     1321 gcacatcata aacgcaagca aaaatagggg aagtgtgata gaatcagccg tcattttttg
     1381 agttaaagat ttagaggttt tatgaaaatt tccccacgtc gccgtgcaag agaatgtgcg
     1441 gtgcaagcca tttattcttg gtatatttcc caaaacacgg ttgaagaagt ggaattggcg
     1501 tttgtgaccg atcaagatat gaaaggcgtt gatatgcctt atttccgtaa acttttccgt
     1561 ggcgttgtgg ataatgtaga agccattgat gaagcattac gcccttattt agatcgtaaa
     1621 gagtcagatg ttgatcctat tgaacgttct attttgcgtt tatcaactta cgaattaaaa
     1681 tttgaacacg atgtgccttt ccgtgtagtc atcaatgaag ggattgaagt cgctaaagtg
     1741 ttcggctctg atgacagcca caaatacatt aacggcattt tagataaatt agccccagca
     1801 ttaggcagaa aataataaga ttccccaatt tatacataaa ttggggattt tgattataac
     1861 gctcaaggag attttttatg ggtgaatttg atattatcga acgctatttt aatgcttctc
     1921 aacgcccacc tcgcaaagat gttttacttt ctatcggcga tgactgtgcg ctcacatcac
     1981 tcaaaccaaa tcagcaactt gcgatcacaa ccgataccct tgtttgcggc actcattttc
     2041 taccgactat ttcacctgcc gatctcgctt ataaaagtgt ggcgtccaat ttaagcgatc
     2101 ttgccgcaat gggtgcagaa cctgcatgga tctctttagc cttaacctta cctgaaatta
     2161 atcacgattg gctagctgaa tttagtgaac atttctttga gattcttgat cactataatg
     2221 ttgatttgat tggaggggat actacttgtg gaccactatc tttgacaatc acagctcaag
     2281 ggattgtgcc acaagataga ggcttattcc gccataatgc taaagtaggc gattggatct
     2341 atgtttccgg cacactcggt gacagtgcag gtggtttgca acttctgcta aatcagcaaa
     2401 gcgagcaagt gaattggaat aaagagcaaa gctacctgat tcaacgccat ttacgcccaa
     2461 cacctcgagt tttacttggc ttgactatcg cctctattgc taatgcagcg attgatattt
     2521 cagatggtct attgtctgat ttaagccata ttttaaagcg cagtgactgt ggtgcagtat
     2581 taaaccttga gaatttcccc ttgtctgacg tcttacttag ttgttattct cgaacagagg
     2641 cagaatattt tgccttaaac ggtggcgaag attacgaact ctgctttacc gtgccaaatg
     2701 acaacaaagc caaattagac aaagcattag ccaatatcgg agtaacttat acatgtattg
     2761 ggcaaattcg ttcggctaaa gaaggtttaa ccctattacg tcataatcag cctgtcacct
     2821 tgccctcaca acgaggctac gatcatttta aaaaataacg caatgaaacc aattaatctt
     2881 aaaaatccga ttcatttttt tgcctttggc tttggttcag gcttattaaa gcctgctccc
     2941 ggcacctggg gtagcctcgc cgggctaatt gtagcaatag ccttgtggtc tatcacacaa
     3001 tccgccctgt ttttcggctt actcacccta gtttctttta ttgccggttg tgttatttgc
     3061 accaaagcca gccaagatct agaagtacac gatgacgggc gaattgtttg ggacgaaata
     3121 gttgccatct ttttgatgtt cacctgtttg cctgaataca atcttcttgg ctacctgctc
     3181 gcctttatca gctttcgcat ttttgatatt ttgaagcctt ttccaatccg ctattttgat
     3241 gaaaaattag aaagcggttt aggcattatg tttgatgata ttctcgcagc catttttgcc
     3301 ctgatttcac ttcatctttt atattatttc atttaaaaat ttatgctcac tattttattt
     3361 attcattttg ccgggcttat cagccccggc cccgatttct tctatgtcgt cagacaatcc
     3421 gccagccact cagttaaaag tggggtactt gccgcaaccg gaatctcaat cggcattatt
     3481 ttttgggcag cctttgcgat ttttggttta gcgtggctca gtaatagtat tggctcgctg
     3541 tttcaatatg ccattatgat tatcggtggc tgttacctgg tttatattgg ctctcaaatg
     3601 gtacgggtaa ccgaaaatat catttttgtt gaaaataaag ctcaactcac gccgctaaac
     3661 ccttggcagg aaattaaaaa aggcttatta atcaatatct ttaatgccaa agctggcgtt
     3721 tactttacca gcgtcatttc agcctatgta gataatttaa gcaataccct acaaatgttc
     3781 gaactacttc tactctttgt ggtaagtacc tttatttatt tctgtgtggt ggcactactc
     3841 ttttctcgcc gaccggtcag actgttctat gccaaatata gtcgctatat tgacaatatc
     3901 tcaggcgtaa ttttcatctt attcggctcg atgctgattt atgaaggatt cagccacttt
     3961 atctaatcac aagcggtctg atttcactaa aaatttgcaa attccgctat caataaatag
     4021 tgttatttta tacagtttct gctatgctac tgccaatttt aactctgaga gaacaaaatg
     4081 attggacgat tacacggcaa aattattgaa aagcaaccgc ctgaaatttt attagacgtg
     4141 caaggggtag gctatgaact actgctcccg atgaccagtt tctacagcct gccacaaatt
     4201 ggcgaagagg cgactatttt cacccattta gtggtgcgag aagatgctca cttgctattc
     4261 ggctttgccc aaaaacaaga tagaactcta ttccgtgaat taattaaaac caacggtgtc
     4321 gggccgaagc tcgcgttggc aattctatca gcaatgtcgg tgtcgcaatt tgccaccgcg
     4381 attgaaaatg aagagctggc aaaattaacc aaaattccgg gaatcggtcg gaaaaccgca
     4441 gaacgcttat tggtggaact caaaggcaaa tttaaaggaa tggcacagag tgatttcttt
     4501 gtggaagtat cgcacgagca aattgtggca agtaatacgc cagacccggc aaatgaagcc
     4561 cttgatgcct taatagccct aggctacaaa cctgccgatg cggaaaaaat gattaagaaa
     4621 gtgaataaat ccggtgcaac gagcgaacag cttattcgtg aggcgttaaa aaattcatta
     4681 tgatattgat agtatagggc gaaagatatt tcgcccctac aatctcattt ttaaaaatat
     4741 atgtgtaggg gcaaatcaat atttgccctc atgagtaata tagggtaata ccattccact
     4801 acaacaataa tctaaatgta tgattgaagc agatagaatc atcagtgcct cgcctaagcg
     4861 tgaggaagaa gttatagacc gagcaatccg cccgaaactg cttgccgact atgtcgggca
     4921 gccttctgtg catgatcaaa tggagatctt catcaaagcc gctaaattgc gggaagaggc
     4981 attagatcac ttgctcattt tcggcccgcc cggtttgggg aaaactaccc ttgccaacat
     5041 tgtggcaaac gaaatggggg tgaatattcg cacaacttca gggccggtgc tggaaaaagc
     5101 aggcgatttg gcagcaatgc tgaccaacct tgaaccttac gatgtgctgt ttattgatga
     5161 gattcaccgc ctttctccag cgattgaaga agtgctttat cctgcaatgg aagattacca
     5221 gctcgatatt atgattgggg aaggacctgc tgctcgttcc atcaagctcg atctaccgcc
     5281 ttttaccctt gtaggggcaa ccacccgtgc cggctcactg acctcaccgt tacgagatcg
     5341 ttttgggatt gtacaacgct tagaatttta ctcagtggac gatcttactc tgattgtcaa
     5401 acgcagcgcc gattgcttaa atctcaattt gtcggcagac ggtgcttatg aagtggctcg
     5461 ccgctcccgt ggcacacccc gtattgccaa ccgcttactc cgccgagttc gggattacgc
     5521 cgatgtgcgt aataatggca ttattacctc agacattgcc aaacaagctc tggcaatgct
     5581 cgatgtggat tcggaaggct ttgattttat ggacattaaa ctgttgcaag cgattgtaga
     5641 acgctttgat ggcgggccag ttggattaga caatcttgcg gcagctatcg gcgaagaacg
     5701 agatacgatt gaagatgtgc tagagcctta tctgattcag caaggctttc tacaacgcac
     5761 accaagaggc agaattgcta ccagcagaac ctatgcccat ttaggtataa cccaaataga
     5821 ttagtcatat ttctaaaggc attgatacat aaataaaaag cggatagtta gtactatccg
     5881 ctttcatttg caaaaaatca ataaaatttg accgcttatc cgctaggctt tttcagtctc
     5941 aaaacgctca aaagctaata ctctgctttc taattttctg agaacgattg tcataatgcc
     6001 ggtaattacc aagtaaataa cccctgccat accataaatt gataatgcgt catactctgt
     6061 accataaagc tggcgagcat agcccataat atccataatc gtaatggttg aagcaagcga
     6121 tgtgccttta aaaactagca caatctcatt actataagaa ggtaaagcac gttttaatgc
     6181 gtaaggcact aaaattttca gtgtatctaa acggctcaaa ccaagggctg cgcaagtttc
     6241 ccattgcccc ttcggaatcg cttttaccgc accgtggaac aacaatgtcg aataggcagc
     6301 actgtttaac gctaaagcca acattgcaca gaaccaagcg tcggataata atacccaagc
     6361 cgcactttct gtaatccatt caaattgccc cgggccacta taaatcaaga aaaattgaat
     6421 taataaaggc gttccggtaa aaagcgtaat aaatccgttt accaaccact ttaacggacg
     6481 attttctaac gagagaataa acgtaagaat gatcgctaaa ataaaggcaa tgatgagcgc
     6541 cacaaaagtg agctgtaggc tggtgccaat accctgtgaa atttcaacta aataatccca
     6601 ccacattaag caactcctcg ctcaaaacgg gtaaatctac cttctaatac tttaatcact
     6661 cgctggctaa ttagagtaat cgctaaataa ataagtgctg ctatcccata ccaagtaaaa
     6721 ggttgatgag tattagcatt aattaactcc gattgacgca ttaaatcatc taccccaatt
     6781 aacgatacca atgccgtatc ttttaacagc actaaccact gattgcttaa gcctggtaaa
     6841 gcgtgtcgcc agacttgagg gagaataatg cgaacaaata cttgcgctcg gcttaaccct
     6901 aacgccgtac cagattccca ctgcccaact ggaatagctt taattgcacc acgtaaagat
     6961 tgggaagcat aagcagcaaa aataataccc agcgcaatag ccccacacca gaaagcattt
     7021 aactccgcaa actcatcggt gatcaagaaa ataacttgga taagaccaaa atagatcaaa
     7081 aataccacta aaatctccgg caacccacgc aataatgtaa ttattatact agttggttta
     7141 cggatacatg caatcttact ggtttcaagt accacaaaaa agattgacaa taacattcca
     7201 atcaagaggc ttgccagcgc aatccctaag gtcatcaggg ttgcgttgac aagtaagttt
     7261 aaaatatcgc tagacattat ttcccagcca tccaagtatt ataaatttgc tcatatttac
     7321 catttgtttt gatggtagct aaagccgaat ttaatttttc aagcaatgca gtgttagatt
     7381 tatttaccgc aataccatat ccattaccaa aaaaggcttt atcgactact ttttcaccca
     7441 cgaaatctaa ctctttttcg gtttttagcc actctgccaa tactggtgta tcaccaaata
     7501 ccatatcaac acgtccattt tttaaatcta acactgctga ttgtaggcta gcgtaaggtt
     7561 ttaaattata ttgtttggca tttttgctta agtattgttg aaaagttgta ccattttgca
     7621 cgccgacatt cttagctgtt tccggtgtaa atttcccctt cattgcgaca taacttactg
     7681 agttttctaa ataagcattg gtaaaggcaa cctgtttttg acgcgcttca gtaatatcca
     7741 tgcctgaaat aattgcatca aaatttttaa attttaatcc cgcaattaag ctatcgaatg
     7801 attgcgtttt aaattcgcaa gttgctttta gttcttcaca taatgctttt gcaatatcta
     7861 catcaaagcc aataacttca cctttttcat ttgttgtttc aaaaggggcg taagaaggct
     7921 ccatcgcaaa agtgatagtt tcttgtgcat tggcagaaag cgttgcaaat gcgatagcgg
     7981 aagctaataa taattttttc attgttgtgt ccttttgttg ttatagatta ctctgagtga
     8041 gataaatatt gagcaaattg ctctgttttc ggtgattcaa aacaacttgc atcaccttgt
     8101 tcaataattg tacctttttc catgtacacc actttgcttg ccaccttacg agcaacgcct
     8161 acttcgtgag tcacaatcac ctgagtaatg cctgtagatt gcaattcttt aataatatca
     8221 actacttgtg cagtaatttc cgggtcaagt gctgcggtag gttcatcaaa taataagacc
     8281 tgtggctgca tcatcagtgc tctggcaatc gccacccgtt gttgctgacc acctgaaaga
     8341 tgcagtggga aacgctcggc aaactcgctc aaacgcaaac gtgccaaatg tccttttgct
     8401 ctggcaaccg cttcttcttt gcttaaccca agcactttca tcggggcttc aattaagttt
     8461 tgcattacgg ttaaatgcgg ccataaatga tactgctgga acaccattcc cacctcacgg
     8521 cgtaacaaac tcaccgcttt gctgtcggtt ttagcagtta aatcaaattg atgatttgca
     8581 atcgccaaag tgcctgattg cggtacttcc attaagttta aggttcgaat tagggtactt
     8641 ttacctgccc cactcgggcc aagcaataca acagtatccc ctttttcaat ctctaagtta
     8701 atgtcaaata aggcttggtt tgagccataa aaaaaattga cattttgaat acgaattgcc
     8761 attcgttaaa atcctactac atttaaaata atgaatgaat actaattatt tttgcataac
     8821 tatgcaatat ttttttcttt tagaa
//
LOCUS       DANLZC010000104         8802 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.104, whole genome shotgun sequence.
ACCESSION   DANLZC010000104 DANLZC010000000
VERSION     DANLZC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.104"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..206)
                     /gene="raiA"
                     /locus_tag="PXE44_001879"
     CDS             complement(<1..206)
                     /gene="raiA"
                     /locus_tag="PXE44_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL5589364.1"
                     /translation="MSLNISSKQMDITPAIRTHIEERLAKLEKYHTQLINPHFMLIKQ
                     PNSFQVEATIGTPLGDLVAKAEHE"
     gene            386..1327
                     /locus_tag="PXE44_001880"
     CDS             386..1327
                     /locus_tag="PXE44_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HDL5589365.1"
                     /translation="MSEKKQLPPFQTQFLHPKYWGLWLGLGVFKLILCLPYPILVKIG
                     LGLGKLFTKLGFGKRRMRIARRNLELCFPHYSAEKVEELVEENMKSVGMAIIETGMAW
                     FWSDKRILKWSKIEGLEHLTQRDQERGILLVGVHFLTLELGARIIGLHHQGVGVYRPN
                     DNPLLDWIQFRGRVRSNKAMLDRKDVRGMIRALKNGEMIWYAPDHDYGRRNSVFVPFF
                     AVEETCTTAGSYMLLRSAPKSLVIPFSPIRNLDYSGYTVKVSPAVDFSQCQNEVETAI
                     LMNRVVEREIMAALTQYMWLHRRFKTRPNEGDQSLYD"
     gene            1423..2310
                     /gene="dapA"
                     /locus_tag="PXE44_001881"
     CDS             1423..2310
                     /gene="dapA"
                     /locus_tag="PXE44_001881"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL5589366.1"
                     /translation="MATPLFHGSIVALVTPMTHGEVDYQDLKKLVEYHIQAGSHAIVS
                     VGTTGESTTLSIDENVKVIKKTVEFADGRIPIIAGTGSNATSEAITMTKLLANSGVAG
                     CLSVVPYYNKPTQEGMYLHYKAIAESTDLPQILYNVPGRTGSDLKPETIGRLAKIENI
                     VAVKEATGDLSRLPLIKQLAGEDFIFLSGDDATGFESMKLGGQGVISVTNNVAAADMA
                     KMCELALAGQFTEAEEINNRLMALHHDLFVESNPIPVKWAAYKLGLISEPNLRLPLTT
                     LSESAQPVVLKALQQAGLI"
     gene            2580..3806
                     /locus_tag="PXE44_001882"
     CDS             2580..3806
                     /locus_tag="PXE44_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDL5589367.1"
                     /translation="MAMPSQRFSLTWVLNLFGTAVGAGVLFLPINAGMGGFYPLIIMT
                     LLVGPMTYLAHRGLTRFVLSSKYKGSDITAVVREHFGEQAGKLITLLYFFAIFPILLI
                     YGVGITNTVSSFMENQLHIAPPSRAVLSFMLIAAMIGVMLLSEQVMLKITTCLVYPLV
                     LILLGLSIYLIPQWNTAALQQMPTTGDFLTTLWLTIPVLVFAFNHSPAISSFALSQQK
                     YYQDDKKAEIESAKVLRSTAFILVLFVMFFVFSCVLTLTPEELAQAKVQNISILSYLA
                     NKFDNPIISYFGPLVAFLAIGSSFFGHYLGAREGLEGLVNQMRKEPIDPSKFRKITAI
                     TFLIILWIVATINPSILGFIESLGGPIIAMILFIMPVYAVYKVPALARFKGEFGHLFV
                     LVMGCIAISAIVYGLL"
     gene            3863..5239
                     /locus_tag="PXE44_001883"
     CDS             3863..5239
                     /locus_tag="PXE44_001883"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDL5589368.1"
                     /translation="MISVFDMFKVGIGPSSSHTAGPMKAAKEFVDELQNRKFLNKIDR
                     LRVDVYGSLSLTGRGHNTDIAIIMGLMGYLPHNVDIERIEQTIENLKQNKQLIIAESS
                     VEPKVIAFDFYEDMPFHRDFLPRHENGVKFSALSGEELLFQNTYYSIGGGFIVDEANF
                     EQVKNSDLSVPFPYQNAADLLAHCKNNGLPLSSLVWKNELALRSRVEISDYLAMIWNT
                     MEACIERGLNTEGLLPGPLKVVRRASGLRRMLEANSKFNTDPMQIIDWVNMFALAVNE
                     ENAAGGRVVTAPTNGACGIVPAVLAYYQKFIAQLNQDTIERYLLTTSVIGSLYKMNAS
                     ISGAEVGCQGEVGVACSMAAAGLTEIMGGSAEQVCMAAEIAMEHNLGLTCDPVGGQVQ
                     VPCIERNAIASVKAINASRMALRRTSSPQVSLDKVIETMYETGKDMNAKYRETSTGGL
                     AIKILPCD"
     gene            complement(5294..6883)
                     /gene="purH"
                     /locus_tag="PXE44_001884"
     CDS             complement(5294..6883)
                     /gene="purH"
                     /locus_tag="PXE44_001884"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL5589369.1"
                     /translation="MAIQQALLSVSDKKGIVEFARGLSARGVKLLSTGGTAKLLAEAG
                     LLVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGTDDEVMNQHGIERIDMVVVNLY
                     PFAQTVANPNCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQHQ
                     NSLTLETRFNLAIKAFEHTAQYDSMIANYFGQKVPPYQGAEEEDLTQPSGQFPRTLNL
                     NFVRKQTMRYGENSHQNAAFYVENELKEASVSTAKQLQGKALSYNNIADTDAALECVK
                     EFSEPACVIVKHANPCGVALGKNILDAYNRAYQTDPTSAFGGIIAFNRELDGETAQTI
                     ADRQFVEVIIAPTVSASAKEVLATKKNVRVLECGEFSEAVKRLDFKRVNGGLLVQDAD
                     QGSVSIDDLKVVSERKPTEAELKDLLFCWKVAKYVKSNAIVYAKDGQTIGIGAGQMSR
                     VYSAKIAGIKAEDEGLQVVGCVMASDAFFPFRDGIDAAAKVGITCVIHPGGSMRDQEV
                     IDAANEHGMAVVLTGMRHFRH"
     gene            7055..7324
                     /locus_tag="PXE44_001885"
     CDS             7055..7324
                     /locus_tag="PXE44_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL5589370.1"
                     /translation="MINHNLADFVFEYFTENNLKAGTLRYRYIKENVIDAYSTRVDDA
                     FQGKGIAGELYNALIAFAQEKGLKIKPSCSYIEVKMQRSHRDLIA"
     gene            complement(7411..8799)
                     /gene="dacB"
                     /locus_tag="PXE44_001886"
     CDS             complement(7411..8799)
                     /gene="dacB"
                     /locus_tag="PXE44_001886"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDL5589371.1"
                     /translation="MIPSITQAEINTEELLNTLPAGTSVSFIAKNLDTNQIITQYQSD
                     IFMLPASTQKVFTALAAKLTLNDDFRFQTALLTNGKVENGVLKGNLIARFSGDPELTS
                     GQIYQLMSKLKQQGINKIEGDLILDPSVFASHDKASGWIWNDLTMCFNAPPAAINVDH
                     NCFYVTLNADQPIGEFAKVNVPSAYPVQVFSSAYIVEPKEAPFCQLDVVVHDNNRYQI
                     KGCMARQSQPFGLSFSVQDPTNYGANMLKAQLKSLKIAFNGQVKEPLTAQNGTLLAEH
                     YSEPLPVLLKKMMKKSDNQIADALFRTVANKQHNRPASFQLGSYVIRQLLKTKANIDF
                     KNSVVADGSGLSRHNQVSSRTMLETLEYIAQNEESLKLFETFPIAGVDGTISGRGSIS
                     TEPLAKNLIAKTGSLKGVYNLAGFMKNARGERIAFVQFINGYSTGELESKTKRAPLNT
                     FENKFYMALFNE"
BASE COUNT         2511 a         1629 c         1875 g         2787 t
ORIGIN      
        1 tcttcatgtt cagctttagc gactaaatca cctaaagggg tgccaattgt tgcttcaact
       61 tggaatgagt taggttgttt tatcagcata aaatgtgggt ttatcaattg ggtgtggtat
      121 ttttctaact tagctaaacg ctcctcaata tgagtacgga ttgcaggggt aatgtccatt
      181 tgtttgcttg aaatatttaa tgacataaca ttttcctctt gtttagttaa ggcttttgcc
      241 gttgatattc taataaccaa aatatgctca tcactccaaa aattcaagtg aaaaatgaaa
      301 taagtttcca aaaatttgat ctacttaaca cttttagcag tttttagcta taatgtaatc
      361 ataatctagt atgtgagaat aattaatgag tgagaaaaaa cagttacctc cattccagac
      421 acaattttta caccctaaat attggggatt gtggctcggt ttaggtgttt ttaagttaat
      481 tttatgtttg ccttatccta ttttagtaaa aattggtctg ggactaggta aattgtttac
      541 taaattgggt tttggtaaac gtcgtatgcg tattgctcgc cgtaatttag agctctgttt
      601 tccccattac tctgctgaga aagtagagga attagtcgaa gaaaatatga aatcggttgg
      661 aatggcgatt attgaaactg gtatggcttg gttttggtcg gataaacgta ttcttaaatg
      721 gtctaaaatc gaagggctag agcatttaac acagcgtgat caggaaaggg gtattttact
      781 tgtaggggtg cattttctga ctttagaact cggtgcaaga attattgggc tacatcatca
      841 aggagtaggg gtttatcgac cgaatgataa tccattatta gattggatac agtttcgagg
      901 acgtgttcgc tcaaataaag cgatgttaga tcgtaaagat gtgcgtggaa tgattcgagc
      961 tttaaaaaat ggcgagatga tatggtacgc gcctgatcac gattatggaa gacgaaatag
     1021 cgtttttgta ccattttttg cagtggagga aacttgtact actgcgggta gttatatgtt
     1081 acttcgttct gccccaaaga gtttagtgat cccgtttagc ccgattcgaa atttggacta
     1141 ttcaggctac acagtaaaag tcagtcctgc cgttgatttt agtcaatgtc aaaatgaagt
     1201 tgaaacggct attttaatga atcgggtggt agaacgtgaa attatggctg cgctaactca
     1261 atatatgtgg ctacatcgcc gttttaaaac tcgcccaaat gaaggtgatc aaagcctata
     1321 tgattaattt taaataattt caaaaaagtg cttgcatatt taagctgaat tttgcaatct
     1381 agcaatgtta tcaagcataa taaaaaaata caggagatga ttatggcgac ccctttattt
     1441 cacggtagta tcgtagcatt agttacacca atgactcatg gtgaggtaga ttatcaggat
     1501 ttaaaaaaat tagttgagta tcatattcaa gcaggttctc acgctattgt atctgttggt
     1561 acaacaggtg aatctaccac attaagcatt gatgaaaatg ttaaggtgat taagaaaacg
     1621 gttgaatttg ctgatggacg tattccaatt attgcaggaa ctggttctaa tgcgaccagt
     1681 gaagctatca ctatgactaa attattagca aacagtggtg tagctggttg tttaagtgta
     1741 gtgccttatt acaacaagcc aacgcaagag gggatgtact tacactacaa agcgattgca
     1801 gaaagtacag atttaccaca aattctgtat aatgtgccgg ggcgaacagg gagtgattta
     1861 aaacctgaaa caatcggtcg cttagcgaaa attgagaata ttgttgctgt gaaagaagca
     1921 acgggtgatt taagccgctt acctttaatt aagcaattag ccggtgaaga ttttattttc
     1981 ttaagtggtg atgatgctac cggttttgag tctatgaaat taggtggtca aggggtgatc
     2041 tcggtcacca ataatgttgc tgctgctgat atggcaaaaa tgtgtgagtt agctcttgcc
     2101 ggtcaattta ctgaagcaga agaaattaat aaccgcttaa tggcgttaca ccatgactta
     2161 tttgtcgaat ctaacccgat tccggtgaaa tgggctgctt ataaattagg tttaattagt
     2221 gagcctaatt tacgtttacc gcttaccaca ctgtctgaat cagcacagcc tgtagtgtta
     2281 aaagcattac aacaagcggg cttaatttag ttattttttg caaatcaaat ttgaaaccgg
     2341 ttaagttaat tctaaccggt tttttcatat ctaaaagaaa tttccacata aaaataaaat
     2401 tctaaatttc tttgatttaa gtaaaattta attttgtttg atggattttt actcgccaaa
     2461 aacactattt tttctgcttt taatagtttg atcaaaaaat acgtaaaatc ttgttgtgtt
     2521 actgtttccc atcgctaata tagcatagct tgttagataa ttctttaaag gagattacta
     2581 tggctatgcc atctcaacgt ttttctctta cttgggtact aaatttattt ggtactgcgg
     2641 taggagccgg cgttttattt ttgccaatta atgcaggtat gggtgggttc tacccactta
     2701 ttattatgac tttgttagtt ggaccaatga catatttagc tcaccgtggc ttaacacgtt
     2761 ttgtgctttc ttcaaaatac aaagggagcg atattacggc tgttgttcgt gaacattttg
     2821 gtgaacaggc cggtaaattg atcacgctac tgtacttctt tgccattttt ccgattttac
     2881 tgatttatgg tgtaggtatt accaataccg tgagttcttt tatggagaat cagcttcata
     2941 tcgcgccacc atctagggct gtattatcgt ttatgctaat agctgctatg atcggagtaa
     3001 tgttattaag tgagcaagtc atgttaaaaa tcacaacttg tcttgtgtat ccattagtgt
     3061 tgattttatt gggcttatct atttatttaa ttccacagtg gaatacagct gccttacagc
     3121 aaatgccaac tacaggagac ttccttacta cactttggct gactattcct gttttagtct
     3181 ttgcttttaa ccattcaccg gctatttctt cttttgcact ttctcaacag aaatattacc
     3241 aagatgacaa gaaggctgaa attgaatcag ctaaagtatt acgttctacc gcctttatct
     3301 tggtgttatt tgtgatgttc tttgtattta gctgtgtatt aactttgaca cctgaagaat
     3361 tagcacaagc taaagtgcaa aatatttcga ttctttctta ccttgcgaat aaatttgata
     3421 atccgattat ttcctatttt ggaccgttgg ttgctttttt ggcgattggt agctctttct
     3481 ttgggcatta tttaggtgcg cgcgaggggt tagaaggatt ggttaatcaa atgcgtaaag
     3541 agccgattga tccaagcaaa ttccgcaaaa ttaccgctat tacttttttg atcattttat
     3601 ggattgttgc gactatcaat ccaagcattt taggcttcat tgaaagcctt ggtgggccga
     3661 ttattgcgat gatcttgttt attatgcctg tctatgccgt atataaagta ccggcacttg
     3721 cacgttttaa aggtgaattt ggccatttat ttgtgcttgt tatgggttgt attgctattt
     3781 cagcaatagt atatgggtta ctgtaatctt tctaaaatca atgtagaata cgccctttta
     3841 ttttctatgt gaatttgtgt ttatgattag tgtatttgat atgtttaaag tagggattgg
     3901 gccttccagt tcccatactg cggggccgat gaaagcggca aaggaatttg tcgatgaatt
     3961 acaaaatcgc aaatttttaa acaaaattga ccgcttgcgg gtagatgtct atggttcatt
     4021 atcactcacc gggcgaggac ataatacgga tattgccatc ataatgggat taatggggta
     4081 tttgcctcat aatgtggata ttgaacgtat tgagcagacg attgaaaatc taaaacaaaa
     4141 taagcaactt atcattgctg aaagcagtgt tgagcctaaa gtgattgcgt tcgattttta
     4201 tgaggatatg ccatttcatc gagatttctt acctcggcac gaaaatggtg tgaaatttag
     4261 tgccttgagc ggtgaagagt tgctatttca aaacacctat tattctattg gtggtggctt
     4321 tattgttgat gaagctaact ttgagcaagt taaaaattca gatctttctg ttccattccc
     4381 atatcagaat gcagcagatt tacttgcaca ttgtaagaat aacgggttac cgctttcttc
     4441 tttagtctgg aaaaatgagc ttgctttacg cagtagagta gaaatttcgg attatttggc
     4501 aatgatttgg aatacaatgg aagcctgtat tgagcgtggt ttgaatacgg aagggttact
     4561 acccgggcct ctgaaagtgg tacgccgtgc cagtgggcta cgcagaatgt tagaggcaaa
     4621 cagtaaattc aataccgacc caatgcaaat tatcgattgg gtgaatatgt ttgcccttgc
     4681 cgtgaatgaa gaaaatgcgg caggtgggcg agtagtcacc gcacctacca atggagcctg
     4741 tggtattgtt ccggctgtgt tggcgtacta ccaaaaattt attgcacagc ttaatcaaga
     4801 tactattgag cgttatttat tgactaccag tgtgattggt tcgttataca aaatgaatgc
     4861 ctctatttcc ggtgctgaag taggttgcca aggggaagtt ggtgttgctt gttcaatggc
     4921 ggcagcaggt ttaacggaaa ttatgggtgg cagtgctgaa caggtgtgta tggctgcaga
     4981 aattgcaatg gagcacaatt taggcctaac ttgtgaccct gttggcggac aagtgcaagt
     5041 gccttgtatt gaaagaaatg caatcgcttc agtcaaagcg attaatgcca gcagaatggc
     5101 actacgccgt acttcttcgc ctcaggtaag tttagataaa gtcattgaaa caatgtacga
     5161 aaccggcaaa gatatgaatg caaaataccg tgaaacctca accggtggct tagcaatcaa
     5221 aattttacct tgtgattaat taatgaaaaa ggctacctta tcaaaataag gtagcctttt
     5281 agtttgtaga acattaatga cggaaatgcc tcattccggt tagtaccact gccataccgt
     5341 gttcgttggc ggcatcaatc acttcttgat cacgcattga accacctggg tgaatcacac
     5401 aagtaatccc tacttttgca gcagcgtcaa taccatcacg gaacgggaag aacgcatcgg
     5461 aagccattac acaacccaca acttgtaagc cctcgtcttc cgctttaata cctgcaattt
     5521 ttgcggaata aacacggctc atctgccctg caccgatacc gatagtttgc ccgtctttgg
     5581 catacacaat cgcattcgat ttcacatatt tcgccacttt ccagcagaac aataggtctt
     5641 ttagctcagc ttcagttggt ttacgctctg aaaccacctt taaatcgtca atagatacag
     5701 agccttggtc ggcatcttgc actaataatc cgccgtttac ccgtttgaaa tctaagcgtt
     5761 ttacagcttc tgagaactcg ccacattcaa gcacacgcac gtttttctta gtggctaaca
     5821 cttctttggc agaagcagaa acggttggtg caataatcac ttcaacaaat tgacggtctg
     5881 cgatggtttg agcagtttcg ccgtctaatt cacggttaaa ggcaataatg ccaccgaaag
     5941 cagaagttgg atcggtttgg taagcacggt tgtaggcatc taaaatattt ttacccaaag
     6001 caacaccaca agggttagcg tgttttacaa tcacacaagc aggctcgcta aattctttca
     6061 cgcattcaag agccgcatcg gtatctgcaa tattattata cgaaagggct ttaccttgta
     6121 actgtttggc ggtggaaaca gaggcttctt ttaactcatt ttcgacatag aatgcagcgt
     6181 tttgatgcga attttcgcca taacgcatcg tttgtttacg cacaaaattg aggtttaagg
     6241 tgcgagggaa ttgtccactt ggttgggtta aatcttcttc ctcagcccct tgataaggcg
     6301 gcactttttg cccgaaatag ttggcaatca ttgaatcgta ttgtgcggta tgctcaaacg
     6361 ctttaatagc aagattaaaa cgggtttcta aagttaaact gttttgatgt tgatccattt
     6421 cggcaagaat agcatcaaaa tcattgttat tgaccacaat cgccacatct ttatgatttt
     6481 tcgctgcaga acgcaccatt gtcggaccac cgatatcaat attttccacc gcatcttcaa
     6541 ggctacaatt tggattggca acggtttgag caaaagggta gaggtttact accaccatat
     6601 caattcgttc aatgccgtgt tggttcatca cttcatcatc agtaccacgg cgacctagaa
     6661 ttccgccgtg cactttcggg tgcagggttt ttacacgccc gtccatcatt tcaggaaagc
     6721 cggtgtaatc tgatacttct gttactagca aaccggcttc agccagtaat tttgcggttc
     6781 cgcccgtaga aagtaatttt accccacgag ccgatagccc acgagcaaat tcgacaatgc
     6841 cttttttatc agacacactt aataacgcct gttgaatcgc cattcttaaa ttccttaaaa
     6901 tgagtgttaa cgtgaaaata aacggcaaag attataacgc aaacgtttgc ttttttcatc
     6961 tacaagtggt cgattttgca aatttttctg caaaatcgac cgcttgcact cgtgattaaa
     7021 gcgggtagaa tagaggaaaa aaatcggagg aactatgatt aaccacaatt tagcagattt
     7081 cgtatttgag tattttaccg aaaacaacct aaaagcaggc acactacgtt atcgctacat
     7141 aaaagagaac gtgattgatg cttacagtac ccgagtagat gatgcctttc aaggtaaagg
     7201 tattgcagga gagctttaca atgctttaat tgcgtttgcg caagaaaaag ggctgaaaat
     7261 caagccaagt tgtagttata ttgaggtaaa aatgcaacga agtcatcgag atttaattgc
     7321 ttagagagga gcaagcggtc gtaaaatttc aaaaatttac gaccgctgtc ttattcaatt
     7381 ctcaaaaatc catcacacaa aatataagcc ctattcatta aacaacgcca tataaaactt
     7441 attttcaaat gtattaagcg gagcccgttt ggttttgctt tcgagctcgc cggtggaata
     7501 gccgttaata aattgcacaa aagcgatgcg ttcgccacgg gcgtttttca taaagccggc
     7561 aaggttgtaa acccctttca gtgagccggt tttggcaatc agatttttgg caagcggttc
     7621 tgttgaaata gagcctctgc cggaaatagt accgtccacg cctgcaatcg ggaaggtttc
     7681 aaacagtttt aagctttctt cgttttgagc gatatattca agggtctcca gcattgttcg
     7741 gctgctgact tgattatgac gggaaagccc tgagccatca gccaccacgc tgtttttaaa
     7801 atcaatattg gctttggttt ttaataactg gcgaatcaca taactaccta attggaacga
     7861 tgcggggcgg ttgtgttgct tgttggcaac ggttctaaac agggcatcgg caatttggtt
     7921 atccgatttt ttcatcattt ttttcaataa taccggcaac ggctcggaat aatgctcggc
     7981 aagtagcgtg ccgttttggg cagtaagtgg ctcttttact tgcccgttaa aggcaatttt
     8041 caggcttttt aactgggctt taagcatatt tgccccgtaa ttagttgggt cttgcaccga
     8101 aaaacttaag ccgaacggtt gagactggcg agccatacag cctttgattt ggtagcggtt
     8161 gttatcgtga accaccacat caagctggca aaacggagct tctttcggct cgacaatata
     8221 agcagagcta aacacctgca ccggataagc ggacggcaca ttcactttag caaattcgcc
     8281 aatgggttgg tcggcattta aggtgacata aaaacagttg tgatccacat taattgccgc
     8341 cggtggggcg ttaaagcaca tcgtcaagtc gttccaaatc caaccggagg ctttgtcgtg
     8401 gctggcgaaa accgacgggt ctagaattaa atccccctca attttattga taccttgttg
     8461 ctttaatttg ctcattaatt gataaatttg accgcttgta agctcagggt cgccactaaa
     8521 acgggcaatt aaattgcctt ttaacactcc gttttctact tttccgttgg ttaaaagggc
     8581 agtttgaaag cggaaatcat cgtttaaagt taattttgct gccagtgccg taaagacttt
     8641 ttgggtactg gcaggtagca taaaaatatc actttggtat tgggtgatga tttggttagt
     8701 gtctaaattt ttagcaataa aactaacaga agtaccggca ggcaaggtat ttaataattc
     8761 ttcggtatta atttcagcct gtgtaatgct tggaatcaga ag
//
LOCUS       DANLZC010000105         8752 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.105, whole genome shotgun sequence.
ACCESSION   DANLZC010000105 DANLZC010000000
VERSION     DANLZC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8752
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.105"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            554..2941
                     /locus_tag="PXE44_001887"
     CDS             554..2941
                     /locus_tag="PXE44_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDL5589372.1"
                     /translation="MEKMMRKINQLIISPYFFLSILSASANSDFWKSDLNFNLTNITK
                     RVGVDNFTTPVAGEPWAGIGPNGEAAGTAPLYYSRIPAMQVTEDNKLVVMFDLRWNRA
                     YDQDRIDPGIAISSDGGHTWTKKTAWSFDRTNHPARRSMDPTLLHNPIDNSLYVMHGT
                     WSMSDQQWYGGRVPHFNSGSWAATIYKSTDGGLNWEKNTEFSKFSNLDVFSKVTKNGK
                     PTLGFLGGVGSGIVMQDGTLVFPIQTAHQNGIATTIMYSKDNGKTWEMPEIDNPVAPN
                     QSSLENMVFELEPGKLVMTGRGNSRWAYSSTDMGKTWELFTPTNGLLPTSSQPTQGSS
                     IYVTLPNGRKVLLISKPDGKNDGWKRGDITLWMLDAKDPSHKHKVHIIRPGSGNAAGA
                     GYSSLAYKEGNLFVAFENDGDISVKNLTEHIAEMEAKAIEWGLPDELTPALDKINALP
                     YLNKAQKETLVEKMRRANDTAIAQSFVINKEMYNLKNNSDELDKLAKNITKALPSQQN
                     IYQRALAYVNKVTNTADTTYLNYNGIMDTYANLYESYLALNTKLDFTRYIQKARKFNE
                     YNTDILYRSFDNFFAHYDTGVKHNNLSLGLNTKLSENLRGGLFFEYSNKNRNSVQIGA
                     RAKYEMDNHQLSGFLRYRGVKHKDMLARNNSVDGYLNYAYRIQLDDKLSISPSVGAYV
                     SRSSRSLIDEDLAINSRTVYASDVGLNIHYKLNDIDAYIRPSIGFVNGDITLSQSNDM
                     TNTYKIKDKSNVYSVTTGLKKRFANGVALGADFKLHRYGSQTTESNFGLNVSYNW"
     gene            complement(3108..3644)
                     /gene="hpt"
                     /locus_tag="PXE44_001888"
     CDS             complement(3108..3644)
                     /gene="hpt"
                     /locus_tag="PXE44_001888"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL5589373.1"
                     /translation="MEKHHIETLHSKEEVLNRINELAKEINQHYHQKQCQNLVVVGLL
                     RGSFMFMADLVRKLELPVEVDFLTASSYGSSTESSRDVKILKDLDGEIQGKDVLIVED
                     IIDTGFTLSKVGEILKLREPNSLAICTLLDKPCRREVDVPVKWVGFEIPDEFVVGYGI
                     DYAQKYRHLDYIAKVVIE"
     gene            complement(3683..5032)
                     /gene="pmbA"
                     /locus_tag="PXE44_001889"
     CDS             complement(3683..5032)
                     /gene="pmbA"
                     /locus_tag="PXE44_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDL5589374.1"
                     /translation="MSITDKQTLLQQEQELRQAVEFALNFAKQAGAEAEVGVTKVAGL
                     SVSSRLEQVENIEFNNDGSLGISVYLGKRKGNASTSDLQPESIQKTVEAALAIAKYTS
                     EDACTGLADKELLAFEAEDLELYHQADISVDQAVELAIEAEHYALNADEKIVNSDGAT
                     FNSHSGVRVYGNTHGMLQSYLSSRYSLSCSVISAYEDQLERDYEYTIFREFDKLQAPK
                     WVGEQAAIKAVDRLNPQRIKTGEMSVIFYNDVATGLVGHLAGAISGGALYRKSSFLLD
                     KLGEQILPEWFAISERPHLLRQLASSAFDSEGVITQNREIITDGVLQTYLMTSYSGRK
                     MGLKSTGHAGGIHNWLVKPNRQGGLNTLLKEMGTGLLVTEMLGSAINSVTGEYSRGAA
                     GFWVENGEIQYPVAEITIAGQLQDMYKNLVAVGDDIEHRSNIQTGSILLEKMMVSGE"
     gene            5131..5661
                     /locus_tag="PXE44_001890"
     CDS             5131..5661
                     /locus_tag="PXE44_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDL5589375.1"
                     /translation="MAKKRSKNEIDWTDEEEEIIWVSKSEIKRDSEHLKKIGAELIEL
                     TPQNLEKIPLDDDLKEAIRQAQGFKMEARRRQIQFIGKLLRNRDQEPIQEALDKVKNR
                     HNQQQALLHKLELVRNQLIAMGDASLDNLLTEYPQLDRQHLRNLIRGAIKEREANKPA
                     KNYREIYQYLKTEIVE"
     gene            5667..6038
                     /locus_tag="PXE44_001891"
     CDS             5667..6038
                     /locus_tag="PXE44_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HDL5589376.1"
                     /translation="MEYLSTILKAHIGLAYISLILLLTRGFLSSKMVDWRQYKILRIA
                     PHAVDTFLLITGIAAVAIFLAYDFFTLAQFSWLLPKLLFLVLYIVFATKAFKKGQPFS
                     LKFYLLAVVSFLLMMLTATFH"
     gene            6051..6764
                     /locus_tag="PXE44_001892"
     CDS             6051..6764
                     /locus_tag="PXE44_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL5589377.1"
                     /translation="MIGPFVNAGAIILGGVLGACLGSHLPERIRTTLPLVFGLCCFGL
                     GMMLTASVKYMSAVALALILGTIIGELIYLENRIGKVAGLARGLIDRLFPPVHSLSQE
                     EFLEKFIALLVLFCVSGTGIFGAIKEGMTGDPSILYIKSILDFFTAMIFAASLGYAIA
                     AIAVPLIIVQGILILLATVIMPLTTANMMADFSATGGLLLFATGFRICGIKNFPVANM
                     LPALLLAMPMSYFWEWLVH"
     gene            6767..7330
                     /locus_tag="PXE44_001893"
     CDS             6767..7330
                     /locus_tag="PXE44_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL5589378.1"
                     /translation="MKKRIIVGISGASGFQYGYKVLELLKPFSEIETHLVISKGAELT
                     RTLETDYTREALHDLADQIHSLNNVGASIASGSFKTEGMVIAPCSMRTLAAIANGLSD
                     NLLTRAADVVLKERRKLVLMVREAPLNLAHIDNMRKVTEMGGIIFPPIPAFYQKPTSL
                     DEMVTHSVSHALSLLDIDIPNTPHWGE"
     gene            7393..7833
                     /locus_tag="PXE44_001894"
     CDS             7393..7833
                     /locus_tag="PXE44_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDL5589379.1"
                     /translation="MHNPIPTHILDFIQTNHVVNIAAHTEQDFWAANCFYAFDQENVR
                     LIILTAKKTRHAGLMLENPHIVGTICGQIEKINEIEGVQFSATAECLEDKQAEKAALQ
                     IYYQKHPLARLKPSDVWVLSFNTIKHTGNKLMFAKKTIWERNII"
     gene            complement(7837..8712)
                     /locus_tag="PXE44_001895"
     CDS             complement(7837..8712)
                     /locus_tag="PXE44_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5589380.1"
                     /translation="MMKTILIDEANQIKGFSHLYRLRSDLMLKYTEISVTETVEQEPA
                     EFEAGLRLVILLSGRSEIDFNSEVFELDASLKPQAAFLPLNRAEMGRKIFRKGQLQKE
                     LVIFLYPKWLIESQLNLASYHHFKQHLKAHQIILTQSMLKLIHDIVSEQEMQPFGLLE
                     KEGNLLALLASAFSQLTQAPRLKKDRVQQLTELLESGKFDGWSLKQIAIYFHTNVTTL
                     QNEFQHYHKTSIFAYLRRSKLEQAYKLLLQGQSITQAAEFAGYANSENFSTAFKRTFG
                     ITPSQIKRDKAKGII"
BASE COUNT         2648 a         1736 c         1623 g         2745 t
ORIGIN      
        1 tgaaaaatca aattataaag aaaccttatc tatcaatact tgaagccatt aagttcaagt
       61 ctcgaccagg caccatttga ctaaatttag ttcaacagag ctaaacgaaa aaaccttaga
      121 aagcctgtaa aatcaacgtt tacaggcttt tttgttgttt tagctcaatg gcactaaaca
      181 tttcatatcg caaattttaa gtagcctagc aaattttccg ccaaatatga ccgcttgtaa
      241 tgccgttagg ttattgcctg gcggcctttt tgttacagct cttatatttt ttacaccaaa
      301 gttcagaaaa agtcactttt atcactaaca ttaagatgaa accttgatac aaagcttggg
      361 cttcggtgat atttaaaaaa tatcactttt ttcaataatt gtgacaaatc tctcagattt
      421 ttttattttt ttgtgcttaa atcttaagca taaaatgccg ttataattac ttgctatctg
      481 gtattttata cataagattg aaccatataa tagctgttca gctaaaaatt agtctttgca
      541 ctttatcccc ctaatggaga aaatgatgag aaaaatcaat cagttaatca tatccccata
      601 ctttttccta agtattttat ctgcttccgc taactctgat ttttggaaaa gcgaccttaa
      661 ttttaatctc acaaatatta ccaagcgtgt cggagtcgat aactttacca ctccagtagc
      721 tggcgagcct tgggctggta ttggtccaaa tggtgaagct gcaggtactg cgccattata
      781 ttatagccgt attccagcta tgcaagttac cgaagataac aaattagttg ttatgtttga
      841 tttacgctgg aatcgtgcgt atgaccaaga tcgtattgat cctggtattg cgatttcttc
      901 cgatggtggc catacttgga cgaagaaaac cgcttggtct tttgatagaa ctaatcaccc
      961 agcacgccgt tcaatggatc caacgctatt acataatcct attgataata gtttatatgt
     1021 aatgcacgga acttggtcta tgtcggatca acaatggtat ggtgggcgtg ttcctcactt
     1081 taatagtggc agctgggcag ctaccattta taaatctact gatggtggtt taaattggga
     1141 gaaaaatact gagtttagta aattcagcaa tttagacgta ttttctaaag tcaccaaaaa
     1201 tggaaaacct accttaggct ttttaggcgg ggtcggatct ggaattgtaa tgcaagatgg
     1261 tacgttggtt ttcccaattc aaaccgcaca tcaaaatggt attgcgacca ctattatgta
     1321 ttctaaggat aatgggaaaa cttgggaaat gccagaaatt gacaacccag ttgcaccgaa
     1381 ccaaagttct ttagaaaata tggtgtttga actcgaaccg ggtaagctag tgatgactgg
     1441 acgaggaaac agtcgctggg catactcttc tactgatatg ggtaagacct gggaattatt
     1501 tactccaact aatggattat taccaactag ctcacaacca actcaaggct cttctatcta
     1561 tgtaacacta ccaaatggtc gaaaagtgct actaatctct aaaccagatg gaaagaatga
     1621 tgggtggaaa aggggtgata ttacactttg gatgctagat gcaaaagatc catcgcacaa
     1681 acacaaggtg catattattc gtccaggttc aggtaatgcc gctggtgctg gttattcctc
     1741 tttagcctat aaagaaggga atttatttgt tgcttttgaa aacgatggtg atatttcagt
     1801 aaaaaattta actgaacata ttgctgaaat ggaagcaaaa gctatcgaat ggggattacc
     1861 agatgaactt actcctgctt tggataaaat caatgcactg ccttatctca ataaagctca
     1921 aaaagagacg ctagtcgaaa aaatgcgtcg tgctaatgat actgcgattg cacaatcttt
     1981 tgtgattaac aaagaaatgt ataatctgaa aaacaatagt gatgagctgg ataaattggc
     2041 aaaaaatatt actaaagcac tgccgtctca acagaatatt tatcaaagag ccttagccta
     2101 tgtgaataaa gtcaccaata cagcggatac cacttattta aattataatg gtattatgga
     2161 tacctatgct aatttatatg aaagctattt ggctttaaat actaaattag acttcactcg
     2221 ctacatccaa aaagcgagaa agttcaatga atataacact gatattttat atcgtagctt
     2281 tgataatttc tttgcccatt acgacacagg tgtaaaacac aataatctat ccttaggctt
     2341 gaatacaaaa ttaagtgaga atttacgtgg tggcttattc tttgaataca gtaataaaaa
     2401 tcgtaatagc gtacaaattg gtgcaagagc taaatatgaa atggataatc accaactttc
     2461 tggcttctta cgctatcgtg gcgtaaaaca taaagatatg ctagcacgta ataacagtgt
     2521 tgatggctac ctaaattatg cttatcgtat tcaattagat gataaattaa gcattagccc
     2581 atcagttggt gcttacgttt cacgttcttc tcgctcatta atagatgaag atcttgcgat
     2641 taatagccgc acggtctatg caagtgatgt aggattgaat atccattata agctaaatga
     2701 tattgatgct tatattcgtc caagtatagg ctttgtcaat ggtgatataa ctttatctca
     2761 atctaatgat atgacaaaca catacaaaat aaaggataaa agtaacgtgt attctgtcac
     2821 gacagggttg aaaaaacgct ttgcaaatgg tgtggcatta ggggctgatt tcaaattaca
     2881 tcgttatggt tctcaaacta ctgaaagtaa tttcggtcta aatgtaagtt ataactggta
     2941 ataccgtaaa ctgattacga tttaaaagtt agactagccc aagactgagt tctatattcc
     3001 atagagctca gtcttttaat ttttctttct cgataagaat attttatatt tcaaccttaa
     3061 tcgagaatgt aattagtcgt gaaaatctaa gtagtgattg agttttttta ttcaatcact
     3121 actttagcaa tataatctaa atgacgatat ttttgggcat aatcaatccc ataacccacc
     3181 acaaattcat cggggatctc aaatcccacc cattttacgg gcacatcaac ttcgcggcgg
     3241 caaggtttat ctaataaagt acaaatagca agtgaatttg gctcacgtaa cttgagaatc
     3301 tcccctacct tgcttaaggt gaagccagta tcaataatat cttctacaat caatacatct
     3361 ttaccttgaa tttcgccatc taaatctttt agaattttga catcacggct ggattccgtg
     3421 ctgctgccat agcttgatgc ggttagaaaa tccacttcta caggtaactc tagctttctt
     3481 actaaatctg ccataaacat aaacgagcct cgcaataaac caaccactac aaggttttgg
     3541 cactgttttt ggtgatagtg ttgattgatt tcttttgcta attcattgat tcggtttaat
     3601 acttcttctt ttgaatgtag cgtttcgata tgatgttttt ccattgattt ctccaaataa
     3661 aattgattac aagcggccgt ttttactcgc ctgaaaccat cattttttct aataagattg
     3721 aacctgtttg gatatttgag cggtgttcaa tatcatcgcc taccgccact aaatttttat
     3781 acatatcttg cagttgccct gcaatggtaa tttccgctac agggtattga atttcaccgt
     3841 tttctaccca aaatcctgct gcacctcgag agtattcccc tgttacgcta ttaatcgcag
     3901 aacctagcat ttccgttact aataatcctg ttcccatttc cttaagtaac gtatttaaac
     3961 caccttgacg atttggttta actaaccagt tatgaatacc acctgcgtgt cctgtacttt
     4021 ttaaccccat tttacgccct gaatagctag tcattaaata cgtttgtagc acgccatcag
     4081 taataatttc acggttttgg gtaattacac cttcactatc aaacgcagaa gaggctaatt
     4141 gacgcaacaa atgcggacgc tctgaaatcg caaaccattc aggcagaatc tgttcgccta
     4201 atttatctaa taagaaactt gatttgcggt ataatgcacc gccactaata gctccggcca
     4261 aatgccccac taaaccggtt gccacatcgt tatagaaaat cacagacatt tcgccggtct
     4321 taattcgttg cggattcaat ctatctaccg ctttaattgc agcttgctcc cctacccatt
     4381 tcggtgcttg taatttatca aactcacgga aaatggtgta ttcataatca cgctcaagct
     4441 gatcttcata agctgaaatc acgctacaag agagtgaata tcggctcgac aggtaactct
     4501 gcaacattcc gtgcgtattg ccataaacac gcacgccact gtgagaattg aaagttgccc
     4561 cgtcactatt cacaattttt tcgtctgcat ttaaggcata atgctccgct tcaatcgcca
     4621 attcgactgc ttgatcaact gaaatatccg cttggtggta caattccaaa tcttctgcct
     4681 caaaggcaag caattcttta tcagcaagcc cggtacaagc atcttctgag gtatattttg
     4741 caatcgcaag agctgcttca accgtttttt gaatcgactc tggctgaaga tcggaggtgg
     4801 aagcattacc tttgcgtttg cctaaataaa ccgaaatacc gagagaacca tcgttattga
     4861 actcgatgtt ttcaacttgc tctaaacggc ttgaaaccga aagcccggca actttagtca
     4921 cgcctacttc cgcctctgcc ccagcctgtt ttgcaaaatt taaggcaaat tcgaccgctt
     4981 gtcgtagctc ctgttcttgt tgtaacaagg tttgtttatc tgtaattgac attttgttcc
     5041 attgattgat tgcattgatg aatatattct aactcaaatt tctgttagaa tacggcattt
     5101 tttagagttt atgtaatgaa aggataaaga atggctaaaa aacggagcaa aaacgaaatt
     5161 gactggacag atgaggaaga agaaatcatc tgggtcagca aaagtgagat aaaacgagat
     5221 tctgaacact taaaaaaaat tggggctgaa ttgatcgaat tgaccccaca aaatttggaa
     5281 aaaattccac tagacgatga tttaaaagag gcaattcgcc aagctcaagg ctttaaaatg
     5341 gaggctcgcc gccgtcaaat tcaatttatc ggcaaattat tacgcaaccg tgaccaggag
     5401 ccgattcaag aagccttaga taaagtgaaa aatcgccata atcagcaaca agccctatta
     5461 cataaattgg aattagtgcg taatcaactg attgcaatgg gcgatgcatc attagataat
     5521 ttattgacgg aatatcctca attagaccgc caacatttgc gtaatttaat tcgtggggca
     5581 attaaagagc gtgaagccaa taaaccggca aaaaattacc gtgaaattta tcaatattta
     5641 aaaactgaaa tcgtggagtg attattatgg agtacctatc aaccattctt aaagctcata
     5701 tcggcttagc ttatattagt ttaattttat tattaacccg tggattttta tcgtcaaaga
     5761 tggtggattg gcgacaatat aaaattttgc gcattgcccc acacgcagtt gataccttct
     5821 tacttatcac cggtattgct gctgtcgcta ttttcttagc ttatgatttt ttcaccttag
     5881 cacaattcag ttggttgcta ccaaagttac tcttcttagt actttatatt gtatttgcga
     5941 caaaagcgtt caaaaaagga caaccgtttt cgctcaaatt ttatctgtta gccgtagtct
     6001 cattcttatt aatgatgcta actgccactt ttcactaaat aagggaaact atgatcgggc
     6061 cttttgtgaa tgccggtgca attattttag ggggcgtgct gggtgcttgt ttagggagtc
     6121 acctgcctga acgtattcgc actacgttgc cgctggtatt cggcttatgc tgtttcggct
     6181 taggtatgat gctgactgcc tcagtaaaat atatgtctgc ggttgcctta gcattgattt
     6241 taggcacgat catcggtgaa ctgatttatt tagaaaacag aatcggtaaa gttgccggtt
     6301 tagctcgtgg tttaattgac cgcctattcc caccggtgca tagtctttcc caagaagagt
     6361 ttttagagaa atttattgct ctgcttgtac tgttttgtgt gagcggtacc ggcatttttg
     6421 gtgcaataaa ggaaggaatg acaggcgatc caagtattct ttacattaaa tcgattctag
     6481 atttcttcac cgcaatgatt ttcgctgctt cgttaggcta tgccattgcg gcgattgcag
     6541 tgccgttgat tattgtgcaa gggattttaa ttctactggc aaccgtgatt atgcctttaa
     6601 ccactgccaa tatgatggca gatttctccg caaccggcgg tttactgcta tttgcaaccg
     6661 gctttcgcat ttgtggaatt aaaaactttc cggtcgcaaa tatgctacca gcactactcc
     6721 ttgcgatgcc gatgtcttat ttctgggaat ggttggtaca ttaatgatga aaaaacgtat
     6781 tattgtcggc attagtgggg caagtggttt tcaatatggc tataaagtgc ttgaactgct
     6841 gaaaccgttt tcagaaattg aaacccacct tgtaatcagc aaaggagctg aactcactcg
     6901 tactctcgaa acggattata cccgagaagc tcttcatgat ttagctgatc aaatacattc
     6961 gctaaacaat gtcggagcaa gcattgccag tggttcattt aaaaccgaag gtatggtcat
     7021 cgccccgtgt tctatgcgaa cccttgccgc tattgccaac ggtttgagcg ataatttgct
     7081 gacccgtgcg gcagatgtgg tgttgaaaga acggcgtaaa ttggtattaa tggtgagaga
     7141 agctccgctc aaccttgctc atattgacaa tatgcgaaaa gtcacagaaa tgggcggcat
     7201 tattttccca cccattccgg cgttttacca aaagccaacc agccttgacg agatggtcac
     7261 ccacagcgta agccatgccc tttccctgtt ggatattgac attccaaata ccccacattg
     7321 gggagaataa ttcggcttat cttttgcttt ttgaaatgct agaatacctg ataaaaacta
     7381 tcaggtattt ttatgcacaa tccaatcccg acccatattt tagatttcat tcaaactaac
     7441 cacgttgtca atattgcagc tcataccgaa caggattttt gggcagcaaa ttgcttttat
     7501 gcctttgacc aagaaaatgt tcgcttgatt atcttaaccg ccaagaaaac cagacacgct
     7561 ggcttgatgc ttgaaaaccc acatattgtc ggcacaattt gtgggcaaat tgagaagatc
     7621 aacgaaattg aaggtgtcca attctccgct accgccgaat gtttagaaga taaacaagcc
     7681 gaaaaagccg ctttgcaaat ttattaccaa aaacacccgc ttgcacggct taagcctagc
     7741 gatgtttggg tgttatcatt caataccatc aagcacaccg gtaataaact tatgtttgcg
     7801 aaaaagacga tttgggagag aaatataatt tgaagctcaa ataatgcctt tagccttatc
     7861 ccgtttgatt tggcttggtg ttatcccaaa agttcgctta aatgccgttg aaaaattctc
     7921 cgaattggca tatccggcaa attcagctgc ttgtgtaata ctctgccctt gtaatagtaa
     7981 tttataggct tgttctaatt tagaacgcct taaatacgca aaaatagagg ttttgtggta
     8041 atgctgaaat tcattttgta atgtggtcac attggtatgg aaatatattg caatctgctt
     8101 caaactccag ccgtcaaatt taccgctctc aagcaattct gttaactgtt gcacccgatc
     8161 tttttttaac cggggagcct gtgtcagctg agaaaaagca cttgctaata aagctaatag
     8221 attaccttct ttctcaagta acccaaatgg ttgcatttcc tgctctgaca caatatcgtg
     8281 aataagtttt aacatactct gagtcaaaat aatttggtgg gcttttaaat gctgtttaaa
     8341 gtgatgatag cttgccaaat ttaactggct ttctattagc catttagggt agagaaagat
     8401 aacaagctct ttttgaagtt gccctttcct aaaaatttta cgccccattt cagctctatt
     8461 tagcggtaaa aatgcggctt gaggttttaa acttgcatct aattcaaata cttccgaatt
     8521 aaaatcaatt tccgatcgac cactcaacaa tataaccaat cttagtccgg cttcaaattc
     8581 cgcaggttct tgctctacgg tttcagtaac tgaaatctct gtatatttca gcataagatc
     8641 tgaacgtagt cggtataaat gtgaaaaacc ttttatttgg ttagcttcat caatcaagat
     8701 tgttttcatc attaaaaact ttttttcgca tagtcaaaat tttattttac aa
//
LOCUS       DANLZC010000106         8719 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.106, whole genome shotgun sequence.
ACCESSION   DANLZC010000106 DANLZC010000000
VERSION     DANLZC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.106"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..357
                     /gene="ssb"
                     /locus_tag="PXE44_001896"
     CDS             <1..357
                     /gene="ssb"
                     /locus_tag="PXE44_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5589381.1"
                     /translation="SEKWTDKQTGVKKESTEWHRVVLYRRLAEIAELYVKKGHLVSII
                     GKIKTRKWTDSNGVERSITEIIAEQMQMLSSGEKNTPNTPNKAENKPQPKQKNQDVMT
                     ADEQKDIPQFDDDIPF"
     gene            368..700
                     /locus_tag="PXE44_001897"
     CDS             368..700
                     /locus_tag="PXE44_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589382.1"
                     /translation="MREWEKQMPFSANLHIRPTGKFEVKNVNIEQIIGERGKNYGNFD
                     DVATMSQQLKAVLLDTNTQLKPHQKEAGEMICLKLARIFAGCNPDYEDNWRDIAGYAV
                     LGGKLNEK"
     gene            690..959
                     /locus_tag="PXE44_001898"
     CDS             690..959
                     /locus_tag="PXE44_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589383.1"
                     /translation="MKNKMGSKFIRVRVEHPQRKKEDIELVQDVVAYHIFNLFKFEES
                     GLRGFSQYDLEIDVKVKPVLEQKEMPVDVWIDDLGGKNGTITSGQ"
     gene            934..1224
                     /locus_tag="PXE44_001899"
     CDS             934..1224
                     /locus_tag="PXE44_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589384.1"
                     /translation="MEQLQAVSNEQVFAKLCEVERLLKTKGVNEHSRELWDLGDVAAY
                     FGYTKEHTSRSVVSSPHFPKPIALDGLRGKGRGAKKWVSGEVVKFCLMWKVK"
     gene            complement(1494..1676)
                     /locus_tag="PXE44_001900"
     CDS             complement(1494..1676)
                     /locus_tag="PXE44_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDL5589385.1"
                     /translation="MAMSRNEIQAKSEAKRGIKQKSFKLPLEVIAEIEVLSQKLNIPQ
                     NQLIIQAIQQFKQNNP"
     gene            complement(1954..2952)
                     /locus_tag="PXE44_001901"
     CDS             complement(1954..2952)
                     /locus_tag="PXE44_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5589386.1"
                     /translation="MATIIKNGNKYRAQVRKKGIYKTATFSTKTEANRWAYALEAQID
                     AGEYNTTPNILFAELIDKYLKEVTPTKRGKREERYRLLRIAGSNLGNIPLPALTKEHL
                     RQWQNQRLQEVQPVSVSRERATLSALFTKAMEWDYLKENPLKTLEKIKTPPARTRRYS
                     DEEIDKLIFVSGYSPNQPPLLMQQRAGAALLFAIETAMRAGEIAGLKWEHINLQARTA
                     FLPQTKNGYSRTVPLSTKAIAILNQLAQLEPNKQNSVFQLTSRSLDSNFRKLKQKANL
                     HNADLHFHDTRREALTRLSKYLTVMELAKVSGHRDLSILQNTYYNPDISELVSKLQ"
     gene            complement(3180..3256)
                     /locus_tag="PXE44_001902"
     tRNA            complement(3180..3256)
                     /locus_tag="PXE44_001902"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3220..3222),aa:Val,seq:gac)
     gene            complement(3320..4120)
                     /gene="apaH"
                     /locus_tag="PXE44_001903"
     CDS             complement(3320..4120)
                     /gene="apaH"
                     /locus_tag="PXE44_001903"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDL5589387.1"
                     /translation="MATYIVGDLHGCFNEFQLLLEKVNYNPKQDELFLTGDLVARGED
                     SLACLRFVKDSANNAKTVLGNHDLHLLGIKRVKPNDKIDAIFEAKDRLDLQNWLRNQP
                     LVIHHPKHQFLLTHAGISPEWDLATTLACAKEAEKVLQSDNYADYIAQMYENKPERWS
                     EDLQGVERWRYIINVFTRMRFCYTDKRLDFDCKLPLEQAPQELLAWFDLDNPLFTQKN
                     IIFGHWASLMGKCTRPNIYALDTGCAWGSHLTMLRWEDKQIFTQKRLK"
     gene            complement(4212..6188)
                     /locus_tag="PXE44_001904"
     CDS             complement(4212..6188)
                     /locus_tag="PXE44_001904"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDL5589388.1"
                     /translation="MFQNNPLLAQLKQQIEASKEYVAGVVKASDKAFGFLECDKKSYF
                     IPPAEMKKVMHGDSVKAVVKRDGDKEQVEIDSLLEPMLDRFIAQVRLNKEGKLQLAVD
                     HPSIKQTIPANTHKKATEKLENGDWVVAQLKTHPLRDDRFFFAQVTQFICKESDNFAP
                     WWVTLARHEQPREPVANEKNYELHDELAREDLSHLYFTTIDSPSTQDMDDALFIEPIK
                     QNGKRTGWRLVVAIADPTAYIPENSHIEKAARQRCFTNYLPGFNIPMLPRELSDDLCS
                     LVPNEKRPALVGFIETDLNGNLIKETTFTSAWVESKDRLAYDDVSDYLEGVENAWQPQ
                     SEETKQQIEWLHQFTQARIQWRAENALLFKEQGDYTFELNEDGSVKDIHVEYRRIANQ
                     MIEESMILANICCAKFLSQHAKTGVFNTHSGFDPKNYELVKSFLLQTLASDENRDELA
                     ERYSAERLATLEGYCQMQRDIQDYPEKFLELRLRRYLTFAEFKAEVAPHFGLGITDYA
                     TWTSPIRKYGDMVNHRLIKQVLLGKQSQAVEESVFSRLQEARRQNRLVERDIADWLYA
                     RYLAPMVEQAVEFECEIADVSRGGLRAKVIKNGAQIFVPFSSLHDNKEEMEYRQEELA
                     LYIKGEKAYQIGQAVKVKLTEVRLETRSIVGNII"
     gene            6348..6626
                     /locus_tag="PXE44_001905"
     CDS             6348..6626
                     /locus_tag="PXE44_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL5589389.1"
                     /translation="MKKLLAIATVFSLAACSTQTLELSPAKEAQYDRVQHFFVSGLAQ
                     KQEIDAAKICNGADKVGKVETETTFFNGLLGTITYGIYSPRQIRVYCN"
     gene            6651..6947
                     /locus_tag="PXE44_001906"
     CDS             6651..6947
                     /locus_tag="PXE44_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL5589390.1"
                     /translation="MKKLLAMSVLTALLTTACTTQTAHIGGKVTDNIKPTKSVTQSFF
                     IGGIGQEETLNVVEVCGSKDKVQQVETVLSGGDILLGLVTIGIYTPRTANVYCK"
     gene            complement(7000..7791)
                     /locus_tag="PXE44_001907"
     CDS             complement(7000..7791)
                     /locus_tag="PXE44_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5589391.1"
                     /translation="MGILTGKRILVTGLASNRSIAYGIANAMKQQGAELAFTYLNDKL
                     KPRVEEFAKEFGSDIVLPLDVATDESITECFTELSKHWEKFDGFVHAIAFAPGDQLDG
                     DYVNAATREGYRIAHDISAYSFVAMAQAARPFLNENAALLTLSYLGAERAIPNYNVMC
                     LAKASLEAATRVMAADLGKEGIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRR
                     TVTIDDVGNSAAFLCSDLASGVTGEVLHVDAGFSVMAMGELGDEE"
     gene            complement(7935..8708)
                     /locus_tag="PXE44_001908"
     CDS             complement(7935..8708)
                     /locus_tag="PXE44_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HDL5589392.1"
                     /translation="MKIVSEPKTSGRHTCTIHCQNCSISQLCLPFTLNETELTQLDNI
                     IERKKPIQKSQVIFKLGDELRSLYAIRSGTLKAYTLSESGEEQITGFHLPGDLIGFDA
                     ITNMQHEGYAQALETSMICEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLL
                     SKMNAEEKLAAFLYNLSQRYSARGFSAKEFRLTMTRGDIGNYLGLTIETISRLLGRFQ
                     KSGMISVQGKYIIINRMEELAELAGAIKPQQNIIAQTTA"
BASE COUNT         2495 a         1591 c         1919 g         2714 t
ORIGIN      
        1 tctgaaaagt ggacagataa gcaaacaggt gtgaagaaag aaagcaccga atggcatagg
       61 gtggtgcttt atcgccgatt agctgaaatc gccgaattgt atgtaaaaaa agggcatttg
      121 gtatcaatta tcgggaaaat taaaacccga aaatggacgg atagcaacgg tgttgagcgg
      181 agtattacag agattattgc cgagcagatg caaatgctca gtagtggtga gaaaaatacg
      241 ccaaatacgc caaataaggc agaaaataaa ccgcaaccaa agcagaaaaa tcaagacgtg
      301 atgactgcgg acgagcagaa agatattccg cagtttgatg atgatattcc gttttagggg
      361 gtgaataatg agagaatggg aaaaacaaat gccatttagt gcaaatttac atataagacc
      421 tacaggaaaa tttgaagtga aaaatgtaaa catagaacaa ataatcggag aacgtggcaa
      481 aaattacggc aactttgatg atgttgccac aatgagccag cagttaaaag cagtgttgct
      541 ggataccaat actcaactta aaccacatca aaaagaggca ggcgagatga tctgcttgaa
      601 actggctcgc atttttgcag gttgtaatcc ggactatgaa gataactggc gagatattgc
      661 gggttatgcg gtgctgggag ggaaattaaa tgaaaaataa aatggggagt aaatttatac
      721 gagtcagagt tgagcatcca cagcggaaaa aagaagatat tgagcttgtg caggatgtgg
      781 ttgcttatca tatttttaat ctttttaaat ttgaggaaag tgggttacgg gggtttagtc
      841 agtatgattt ggaaattgat gtgaaagtta agccagtgtt agaacaaaaa gaaatgcctg
      901 tagatgtttg gattgatgat ttaggtggta aaaatggaac aattacaagc ggtcagtaat
      961 gagcaagttt ttgcaaaact ttgtgaggtt gagcggttat taaaaaccaa aggggtgaat
     1021 gagcatagcc gtgaattgtg ggatttgggc gatgtagcag catatttcgg ctacactaaa
     1081 gaacacacca gccgaagtgt tgtatctagt ccacattttc cgaaaccgat tgcccttgac
     1141 ggcttacgag gcaagggcag gggggcgaag aagtgggtat ccggtgaagt ggtgaaattt
     1201 tgtttgatgt ggaaggtgaa gtgatgataa aaaagataat tttagatgct tgttgcggta
     1261 gccgaatgtt tcactttaat aagcacaacc cctcggtatt gtttgctgac aatcgggaat
     1321 ccctgtatcg aaaattttaa atgttatcga ataccaacct atctatgggc ataaaagcgg
     1381 taaacataac aaaacgcatt ggctggcatt tgtaaagatg gataataacg attatttgta
     1441 aagtatttca aaatacaaaa gccgataaat ttcattattt atcggctttt gttttatggg
     1501 ttgttttgct tgaattgttg aatagcttga ataatcagct ggttttgtgg aatgttgagt
     1561 ttttggctaa gtacttcgat ttctgcgatc acctcaagcg gtagtttaaa ggatttttgc
     1621 tttattcctc gttttgcctc gcttttagct tggatttcat ttcttgacat tgccataatt
     1681 tatccctatt ataaattttg ttgaagagct gggagctttc acccccagcg agttgcttca
     1741 gattagtaag ctggggagct aaggactaat aagaagacaa ctaggataat gtatttaaac
     1801 attgtgctat ccttcttcat ttcgtaaggt ttaagcctta cgctcacttt caagctgtct
     1861 cttgaaagtg aggttattat aatgtagggt atcctacatt gcaagtaata aatacaaaaa
     1921 agccgataaa ttttcgttta tcggcttttg tttttattgc agtttgctca caagttctga
     1981 aatatccggg ttatagtagg tattttgcaa aatacttaaa tctcgatgcc ccgatacttt
     2041 ggcaagctcc attaccgtca gatatttaga caaacgtgtg agtgcctctc gccgtgtgtc
     2101 gtgaaaatgc agatcggcat tgtgtagatt ggctttttgt ttcagcttgc gaaaattcga
     2161 atcaaggctg cgactggtga gctggaaaac actgttttgc ttgttcggtt caagttgtgc
     2221 caactgattt aaaattgcta ttgctttggt tgagagtggc acggtgcgtg aataaccgtt
     2281 tttggtttgt ggcaaaaatg cggtgcgtgc ctgcaggttt atatgttccc attttaaacc
     2341 agcaatttca cccgctcgca ttgcagtctc aatcgcaaat aatagtgctg ctccggctct
     2401 ttgttgcatt aatagcggtg gctggttagg gctgtagccg gagacaaaaa tcagtttgtc
     2461 tatctcctca tcgctatatc ggcgagttct tgctggtggc gttttgattt tttccagcgt
     2521 ttttagagga ttttctttga gataatccca ttccattgct tttgtaaaga gtgccgataa
     2581 agtggctctt tctcttgaga cggaaaccgg ctgcacttct tgcaagcgtt ggttttgcca
     2641 ttggcgtaaa tgctctttgg tgagtgccgg taatggtata tttcccaagt tgctgccggc
     2701 aattcttagc aggcgatagc gttcttccct tttgccacgt ttggtcggag tgacctcttt
     2761 gaggtatttg tcgattaatt cggcaaaaag aatgttcggg gtggtgttat attctccggc
     2821 atcaatttga gcctctaaag cgtaagccca gcggtttgcc tctgttttgg tgctgaaagt
     2881 ggcagtttta taaatgcctt ttttgcggac ttgtgctcgg tatttgttgc cgttcttaat
     2941 aatagtagcc atttttttat tcctttgtgt atgtgtgcag tttaagtgtg caaatgtgtg
     3001 caaaggaatg ttaatagaga tcataaacga tcaataaata tcaagttttc atatcaaaaa
     3061 tgatgttttt attgatgtta tgctacactt ggggaagtgt tttaaatcaa taagctattg
     3121 atttataagg ggtatttttg gggttaagcc tgaatgggga tattcaggct taaaaataat
     3181 ggtgcgacta gctggactcg aaccagtgac ccccaccatg tcaaggtggt gctctaacca
     3241 actgagctat agtcgcatta acggtgtgca ttctattgga aaaaggaact tagtgcaata
     3301 tgcttttggt cgaatgcttt tattttagac gcttttgggt aaaaatttgt ttatcttccc
     3361 aacggagcat ggttaaatgg cttccccaag cacagccggt atcaagtgca tagatgtttg
     3421 gacgagtgca tttgcccatt aggcttgccc agtgaccaaa gataatgttt ttctgtgtaa
     3481 ataacggatt atctaagtca aaccaagcca gcagttcttg aggggcttgt tcaagcggta
     3541 atttacagtc aaaatctaac cgcttgtcgg tgtagcaaaa ccgcatacgg gtaaacacat
     3601 tgatgatata acgccaacgt tcaacacctt gcaaatcttc actccagcgt tcgggcttat
     3661 tctcatacat ttgggcgatg tagtccgcat aattgtcact ttgtagcacc ttttctgcct
     3721 ctttggcaca agctagggta gttgctaaat cccactcagg gctgattcct gcgtgagtca
     3781 gcaaaaattg atgtttgggg tgatgaatta caagcggttg atttctgagc caattttgca
     3841 aatcgagccg atctttagcc tcaaaaatag catcaatctt atcatttggt tttacccgtt
     3901 tgatgccaag caagtgcaaa tcgtggttgc ctagcacggt tttggcattg tttgcagagt
     3961 ctttcacaaa acgtaaacag gctaaagagt cctcaccacg ggcgactaaa tcccctgtta
     4021 aaaagagttc gtcttgcttg gggttataat tgactttttc taataaaagc tgaaattcgt
     4081 taaagcaacc gtgtaggtcg cctacaatat aagttgccat tgtttctcct ttgttcccct
     4141 aaagttataa acaagcggtt atattttaga gaaaatttgc aaaatctcat caaaatataa
     4201 ccgcttgaaa tttaaatgat atttcctacg attgagcggg tttctaagcg tacttcggtg
     4261 agtttcactt tcaccgcttg cccgatctga taggcttttt cacctttaat ataaagtgca
     4321 agttcttctt ggcggtattc catttcctct ttgttgtcgt gcaagctgga gaatggcaca
     4381 aaaatttgag caccgttttt tatgactttc gcacgcaagc caccacggga aacatctgca
     4441 atttcgcact caaattcgac cgcttgttcc accattgggg caaggtaacg agcataaagc
     4501 cagtcagcaa tatcacgttc tactaaacgg ttttgacggc gagcttcttg taaacggctg
     4561 aaaactgatt cttccaccgc ttggctttgt ttgccaagta acacttgctt gattaaacgg
     4621 tggtttacca tatcgccata tttacggatg ggtgatgtcc aagtggcgta atcggtaatg
     4681 cctagtccga aatgcggggc aacttcggct ttaaattcag cgaaggttaa ataacgtcgt
     4741 aaacgcaact ctaagaattt ctctgggtag tcttgaatat cacgttgcat ttggcaatag
     4801 ccttctaagg ttgctaaacg ctcggcggag tagcgttcgg caagctcatc acgattttca
     4861 tcgctggcta aagtttggag taaaaatgat tttaccaatt cataattttt cggatcaaaa
     4921 cctgaatggg tattaaatac accggttttg gcatgttggc ttaagaattt tgcacaacaa
     4981 atattggcaa gaatcattga ttcttcgatc atttgattcg caatacggcg atattcaacg
     5041 tgaatatctt ttaccgagcc atcttcattt aactcaaagg tataatcgcc ttgttctttg
     5101 aatagcaacg cattctcggc acgccattga atgcgggctt gagtaaattg atgtagccac
     5161 tcaatttgtt gttttgtttc ttcagactga ggttgccacg cattttccac gccttcgaga
     5221 taatcggaaa catcgtcgta agccaagcga tcttttgatt cgacccaagc agaggtaaag
     5281 gttgtctctt taatgagatt gccgtttaaa tcggtttcaa taaagccaac gagggcaggg
     5341 cgtttttcat tcgggacaag cgaacagaga tcgtctgaga gttctcgagg taacattgga
     5401 atgttaaagc ccggcaaata gttggtgaaa caacgctggc gagcggcttt ttcaatatgg
     5461 gaattttctg ggatataagc ggttgggtca gcaatcgcta ccactaaacg ccagccggtt
     5521 cgcttgccat tttgcttaat tggttcgatg aacagagcat cgtccatatc ttgcgtgctt
     5581 gggctgtcaa tagtggtaaa gtaaagatga gataaatctt cacgagctaa ttcatcgtgt
     5641 aattcatagt ttttttcatt tgcgaccggt tcacgaggct gttcgtggcg ggcaagtgtg
     5701 acccaccaag gggcaaaatt atcagattct ttacagataa attgggtaac ttgagcaaag
     5761 aaaaaacggt cgtcacgcaa tgggtgcgtt tttaattgtg caaccaccca gtcgccattt
     5821 tctaattttt cagtggcttt tttatgggta tttgccggga tagtttgctt aatgcttggg
     5881 tgatcaaccg caagttgcaa tttgccctct ttattcaagc gaacttgggc aataaatcta
     5941 tccagcattg gctcaagtag tgaatcaatt tctacctgtt ctttgtcgcc atcacgtttg
     6001 actacggctt tcactgaatc accgtgcatt acttttttca tttccgcagg tggaataaaa
     6061 tagctttttt tatcgcattc taaaaaaccg aatgctttgt ctgaggcttt gacaacgcct
     6121 gcgacatatt ctttgcttgc ttcaatttgt tgtttgagtt gggctaaaag gggattgttt
     6181 tgaaacataa tttctcattt taaaaataaa aaaaggtgct atcgttgcac cgaaaaaaac
     6241 aatggcatta taacggattt gaaccaagtt cagcaaataa atgctgttgc tataacttat
     6301 aaaggttggt agaatataat ccatttaaaa ataatggaga cttttcaatg aaaaaattac
     6361 ttgcaattgc aacagtattt tctttagcag cctgttctac tcaaacactt gaactatctc
     6421 ctgctaagga agctcaatat gatcgtgttc aacacttctt tgtttcaggg cttgctcaaa
     6481 aacaagagat agatgcagca aaaatttgta atggtgcaga taaggtaggt aaggtagaaa
     6541 ctgaaactac tttctttaat ggcttgttag gtacaattac ttatggtatc tattcacctc
     6601 gccaaatccg tgtatattgt aactaatcct agtaatttaa ggagttgaaa atgaaaaaat
     6661 tgcttgctat gtctgtatta acagccctgc tgactacagc atgtacaaca caaactgccc
     6721 atattggcgg caaagttact gataatatta aaccaactaa gagtgtaact cagtcgttct
     6781 ttataggtgg gattgggcaa gaagaaacat tgaatgttgt agaggtttgt ggttccaaag
     6841 ataaagtgca gcaggttgaa acagtacttt caggtgggga cattctatta ggtcttgtga
     6901 ctatcggtat ttatacacct cgtactgcaa atgtatattg taagtaatat tttattaaaa
     6961 aaataacgga ctcaattgag tccgttattt ttataggaat tactcttcgt cgcctaattc
     7021 acccattgcc attacgctga agccggcatc aacgtgtaac acttcacctg ttacaccaga
     7081 ggctaaatct gagcataaga aagctgcaga gttacctaca tcatcaatag ttacggtgcg
     7141 gcgtaaagca gcggttttct cgaaagcaga aagcattttc ttgaagtttt taatgcctga
     7201 agcagctaat gtacggatcg ggccggctga aatcgcattt acacgaatac cttctttacc
     7261 taaatcggct gccattacac gggttgcagc ttctaaagag gctttggcta agcacattac
     7321 attgtagttg gggattgcac gctctgcacc taagtaactt aaggttaaaa gggcggcatt
     7381 ttcatttaag aaggggcgag cagcttgagc cattgcaaca aagctatagg cactgatgtc
     7441 gtgtgcaata cgataacctt cacgggttgc ggcatttaca taatcgccgt ctaattgatc
     7501 gcccggtgca aatgcgatag cgtgaacaaa accgtcaaat ttttcccaat gtttgcttaa
     7561 ttcagtaaag cactcagtaa tgctttcatc ggttgctaca tctaacggaa ggacaatgtc
     7621 tgagccaaat tctttggcaa attcttcaac tcgtggtttt aatttatcgt ttaagtaggt
     7681 gaatgcaagt tctgcacctt gttgcttcat tgcgtttgca ataccgtaag cgatcgaacg
     7741 gttgcttgct aaacctgtca ctaaaatacg tttaccagtt aagataccca taaaaattcc
     7801 tcttttatat tgattggggc aaaattgtgt cgattataac ggatatagga aaaacgggca
     7861 aatggagtta gcggctcatc ggatcagaaa tccctttttg aacgaggttt caaaaaggga
     7921 tttggtaaga taaattaagc ggtagtttga gcaataatat tttgctgcgg tttgattgcc
     7981 ccggctaatt cggctaattc ttccatacgg ttaataataa tatattttcc ttgtactgaa
     8041 atcataccgc ttttttggaa acgccctaat aaacggctga tagtttcaat ggttaaacca
     8101 agataattgc cgatatctcc acgggtcata gttagacgga actcttttgc agagaaacca
     8161 cgggctgagt aacgttggga aagattatac aaaaatgcag ctagtttttc ttccgcattc
     8221 atttttgaga gcaatagaat catttcttga tcgcttttaa tttcattact catcaaacgc
     8281 ataatttgat ggcgaatttt tggcattttg ccggcaagat catctaaaat atcgaatgga
     8341 atctcacaaa tcattgaggt ttcgagtgct tgagcatagc cttcgtgctg catattggta
     8401 attgcatcaa agccaattag atcgccaggt aaatggaaac cggtaatttg ctcttcacca
     8461 ctttcactta aggtataggc ttttaatgtt ccagaacgga tagcataaag cgaacgcagt
     8521 tcatcaccta acttaaaaat aacttgcgat ttttgaatcg gttttttacg ctcaataata
     8581 ttatctagtt gagttaattc cgtttcatta agagtaaaag gtaagcatag ttggctaata
     8641 ctgcaatttt gacaatgaat tgtgcaagta tgacgtccgc ttgttttagg ttctgataca
     8701 attttcatcc attcaacct
//
LOCUS       DANLZC010000107         8610 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.107, whole genome shotgun sequence.
ACCESSION   DANLZC010000107 DANLZC010000000
VERSION     DANLZC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8610
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.107"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            43..312
                     /locus_tag="PXE44_001909"
     CDS             43..312
                     /locus_tag="PXE44_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL5589393.1"
                     /translation="MKIMAVCGSGLGSSFMMEMNIKKVLAKLGVEAEVSHTDLSSVVA
                     DDADLFVMAKDIAASSNVPKEKLIVVKNIVGLAEFEEKLTAYFNQ"
     gene            325..1675
                     /locus_tag="PXE44_001910"
                     /pseudo
     CDS             325..1675
                     /locus_tag="PXE44_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013745980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            complement(1770..2918)
                     /gene="dprA"
                     /locus_tag="PXE44_001911"
     CDS             complement(1770..2918)
                     /gene="dprA"
                     /locus_tag="PXE44_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL5589394.1"
                     /translation="MAYHYDLLRLLQVPQMGAQRIGRLLAELNFAEFCHYDKLQLKQI
                     GWNEKQIQRWFNPDQKWIDQAMTWSELPNQQIVTLFDENYPFLLKQISTAPPLIYVKG
                     NVESLSLPQIAIVGSRDYSHYGEYWAGQFAAELARHNIIITSGLAIGIDGFAHQKIVM
                     EQGITIAVLGSGLNQVYPARHKKLAEQIVDLGGALVSEFSPNQPPVAENFPRRNRIIS
                     GLSLGTLVIEATLNSGSLITARYALEQGREVFALPNAVQNPYAQGCHKLIKEGALLVE
                     GVEDILEVVASQYQPQQIPLFDESKPTVSTSLQAVKNSEKFAKNLPSLTACQQQIYQH
                     ISLEPMAIDDLAKVTELTIEILLVELLGLELAGVIKQVSGGYVMDNGY"
     gene            3007..3519
                     /gene="def"
                     /locus_tag="PXE44_001912"
     CDS             3007..3519
                     /gene="def"
                     /locus_tag="PXE44_001912"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL5589395.1"
                     /translation="MAILDVVLYPDEKLATVCEPVEQVDEALNRFIDDMFETMYEHEG
                     IGLAAPQVGVLKRVITIDIEGDKTNQVVLINPEILESSGETGIEEGCLSIPGHRALVP
                     RKEKVKVKALNRQGEEIIIDADDLFAICIQHEIDHLNGVLFVDHISNLKRQRIKEKMQ
                     KLKKQIARAK"
     gene            3613..4539
                     /gene="rfaD"
                     /locus_tag="PXE44_001913"
     CDS             3613..4539
                     /gene="rfaD"
                     /locus_tag="PXE44_001913"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDL5589396.1"
                     /translation="MIIVTGGAGMIGSNIVKALNEIGRKDILVVDNLKNGEKFINLVD
                     LDIADYCDKEDFIASIIAGDDFGEIDAVFHQGACSATTEWDGKYLMQNNYEYSKELLH
                     FCLDRQIPFFYASSAATYGGRSDNFIEKREFEGPLNAYGYSKFLFDEYVRRILPEAES
                     PVCGFKYFNVYGPREQHKGSMASVAFHLNNQMLKGENPKLFAGSEQFLRDFVYVEDVA
                     KVNLWAWQNSISGIFNLGTGKAESFQEVANAVIKYHGKGEIETIPFPDHLKSRYQTFT
                     QADLTNLRAAGYTGTFKTVAEGTAEYMAWLNR"
     gene            complement(4607..5137)
                     /gene="fabA"
                     /locus_tag="PXE44_001914"
     CDS             complement(4607..5137)
                     /gene="fabA"
                     /locus_tag="PXE44_001914"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDL5589397.1"
                     /translation="MNSCTPIIKPSYTYEELLLSGKGELFGEKGPQLPAPPMLMMDRI
                     IEMNQEKGNFEKGYIEAEFDIKPDLWFFNCHFINDPVMPGCLGLDAMWQLVGFYLGWI
                     GGEGKGRALGVGEVKFTGQILPTAKKVTYRIHMKRVVNRKLVMGVADGEVEVDGRVIY
                     TATDLKVGLFQDTTAF"
     gene            complement(5250..6887)
                     /locus_tag="PXE44_001915"
     CDS             complement(5250..6887)
                     /locus_tag="PXE44_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5589398.1"
                     /translation="MTLSLLSSQKLVMQNDFSRLDEKLNFLDFQPQAKQALNQFLQSQ
                     FGALLVLKSELFPEFINEITEYLKKYSVDLPIISKMDFNKNSLFGYSVFLERELKTEY
                     VKGAIHEADKGILIVNIRSLLLDITQWDKLKQALLFGEYEPNSVNHSPYLSAKEKSTF
                     KLILVGEREDISTLSAYDDGLYQFGQYTEIKSYLSLSDNVEQWGGYVQNYAENLINKR
                     LTSTALNQFLQAYIRESESRELVSISPTLLRKHLLGIQNFYPNVTEIDEVKGYFDYLE
                     QQSSILNQYTVGDILTNQLYIETEGEEIGQINGLSVIEFEGIPHSFGEPLRMSCNVQY
                     GDGEITDIERKVELGGNIHSKGIIIAQSCLANLLELPTQLPFSASIAFEQSYGEIDGD
                     SSSLAIFNVLVSALAKVGLPQSIAVTGAIDQFGNVLSVGGVNQKIEGFFNICHARQLT
                     GKQGVIIPAACVSHLSLSSEVVEAVEQGLFKIWTVENVFDAIAILFERDFYDDEKAPN
                     SAQTALFTLIHQNLDERESEETSGSFLSKICKKIKLD"
     gene            6980..7426
                     /gene="matP"
                     /locus_tag="PXE44_001916"
     CDS             6980..7426
                     /gene="matP"
                     /locus_tag="PXE44_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDL5589399.1"
                     /translation="MRYQKLITQETTWKWQYLLKKHREGENITKYEEQSLIDLKVQFL
                     STLQNSPDEVEKWIKSEMTPEQRKKMRQSVRAKRKRFFNAEKQTTKKKSIDLEYSSWL
                     RLSKYAKSQKLTLSEAIVKLTDELENKQLYLEQVAKMKSSLKDLLK"
     gene            complement(7492..>8610)
                     /gene="ompA"
                     /locus_tag="PXE44_001917"
     CDS             complement(7492..>8610)
                     /gene="ompA"
                     /locus_tag="PXE44_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_119863613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDL5589400.1"
                     /translation="ALAVLSAAAVAQAAPQANTFYAGAKAGWASFHDGLTQFDNTYDY
                     GSGINLGINLGINRNSVTYGVFGGYQILNQNNFGLATELGYDYFGRVRGNTTFSGDDK
                     EKTAAKHSAHGAHLSLKPSYEVVPNLDVYGKVGVALVRNDYYVQQHVDKDSRIKVHNL
                     KPSLLLGAGLEYAITPELAARVEYQYLNRVGNLDKAVRTTVGYLIPRGTNFQYSPDIH
                     SVSAGLSYRFGQGAAPVEAPEVVTKNFAFSSDVLFDFGKSSLKPAAATSLDAAHAEIS
                     NLGLANLAIQVNGYTDRIGKEASNLKLSQRRAETVANYIVSKGTNPANVTAVGYGEAN
                     PVTGHTCDAVKGRKALIACLAPDRRVEIQVQGSKEVTM"
BASE COUNT         2592 a         1783 c         1648 g         2587 t
ORIGIN      
        1 aaattcagac aactattcat tcacctttaa tggagattta ctatgaaaat tatggcagta
       61 tgtggttccg gcttaggtag cagctttatg atggaaatga acattaaaaa agtattggca
      121 aaactcggtg tcgaagcgga agtgagtcat accgaccttt cctctgttgt tgctgatgat
      181 gctgatttat ttgtgatggc aaaagatatt gccgcaagca gcaatgtgcc gaaagaaaaa
      241 ctcatcgtgg taaaaaacat tgttggatta gcagaattcg aagaaaaatt gaccgcttac
      301 tttaaccaat aacggagatg ttatatggaa tcgctactct tttttatatt agatattctc
      361 aaagtgcctt ccgttctagt gggtttaatt gctctggtcg gtttagttgc acagaaaaaa
      421 tcggtatccg atattgttaa aggaacaatc aaaactattt taggcttctt agtgttaagc
      481 ggtggtgcaa cagtactttt aagctccctg actcctttag gcggtatgtt tgaacacgcc
      541 ttcaacgtgc aaggcattat ccctaataac gaagcgattg tgtctatggc aatcgaaaaa
      601 tatggtacgg cgactgcgtt aattatggca ttcggtatgg tagccaatat tttaattgca
      661 cgttttaccc gcctaaaatt cattttctta accggacacc ataccttcta tatggcgtgt
      721 atgatcggcg taatcctaac cgttgccggc tttgaaggta ttcagctcgt ctttgtcggg
      781 gcattaacct tagggttaat tatgtttccc gactatcgct cacttctata tgaaaaaagt
      841 gaccgctagc aatgatgttg ccttaggtca tttcggcaca ttgggctacg tgcttgctgg
      901 ttcaatcggg caactggtag gaaaaggctc taaatctacc gaagaaatgg atttacctaa
      961 aaacctcggc ttcttacgtg atagctccat ttctatttca ctcacgatga tggcgattta
     1021 ctatattctc gcccttgctt cgggcagtga atacgtttct acattaagcg gcggacaaca
     1081 ttatttagtg tatgccacca ttcaagccat cacttttgca gccggtgttt atgtcatttt
     1141 gcaaggggtt cgtttaattt tagccgagat cgtcccggca tttaccggct tctccgaaaa
     1201 attagtacct gatgccaaac cggctctcga ctgcccgatt gttttccctt acgcccccaa
     1261 tgcggtatta gttggatttc ttgccagttt cttaggtggg attttaagct tagccattct
     1321 agggcaactt aattgggtgt taattttacc gggcgtagtg ccacacttct tctgtggggc
     1381 aactgccggc gtatttggga acgccaccgg cgggcgtcgt ggtgcaatta tcggtgcttt
     1441 tattcatggc atattaatta ccttcttgcc tgtattctta ttacctgtac tgggttcatt
     1501 aggctttgct aacaccactt tctctgactc agacttcggc ggtattggga ttgtgttagg
     1561 ctatatggct caatacttcg acaagaatat cattacgctc attattgtcg gcttattcgc
     1621 tctgttggta gcctataatt atttggcgaa aaaaccggca aaactgaata cctaattaaa
     1681 atcaggtgtt atttgctgcc tgcgaggaat cttccctgcc atttcttgat ggtagggaat
     1741 tttgtttagt taaacgacaa cgctcttgtt caatacccat tatccatcac atacccccca
     1801 ctcacttgtt taatcacacc ggctaattcc agacccagta attcaaccag caatatttca
     1861 atcgtcaact ccgtaacttt ggctaaatca tcaattgcca tcggctctaa gctgatatgc
     1921 tgataaattt gctgctggca agcggtcaga cttggcagat tttttgcgaa tttttcgctg
     1981 tttttgaccg cttgtaaaga tgtgcttact gttggcttag actcatcaaa caaagggatt
     2041 tgctgaggtt gatattgcga ggcgaccacc tctaaaatat cttccacacc ttccacgagc
     2101 aatgcccctt ctttaattag cttgtggcaa ccttgtgcgt aagggttttg taccgcattc
     2161 ggtaacgcaa acacttccct accttgttca agggcataac gagcggtaat cagtgaacca
     2221 ctattaagcg ttgcttcaat aactaaagtg ccgagcgata agccactaat aattcgatta
     2281 cgacgaggaa aattttctgc caccggcggc tgattaggcg agaactccga caccaatgca
     2341 ccacctaaat caacaatttg ttcggctaat tttttatggc gagcaggata gacttgattt
     2401 aatccactcc ctaacacggc aatcgttatg ccttgttcca taacgatttt ttgatgggca
     2461 aaaccgtcaa taccaatggc taaaccgctg gtaatgataa tattatggcg agctaattcg
     2521 gcagcaaatt gcccagccca atattcacca taatgagaat agtctcggct gccaacaatc
     2581 gcaatttgtg gcagagaaag gctttccaca tttcccttta cataaattaa cggtggcgca
     2641 gtcgaaattt gttttaagag aaacggataa ttttcatcaa agagtgtcac aatttgctga
     2701 tttggcaatt ctgaccaagt catcgcctga tcaatccact tttgatcagg attaaaccaa
     2761 cgttgaattt gcttttcatt ccaacctatt tgtttgagtt gaagtttgtc atagtggcaa
     2821 aattcagcaa aattgagttc cgccaataat cgccctatac gttgagcccc catttgcggc
     2881 acttgtaata atcgtaataa atcgtagtga tatgccatat tttcatcctc aaaatttccc
     2941 taataataag aaaaggttta aaatagccaa acgaattttt taaacttttc gagagagatc
     3001 acaaaaatgg caatattaga tgttgtgctt taccccgatg agaaactggc aaccgtttgt
     3061 gaaccggtgg aacaggtgga cgaagcactg aatcgtttta tcgatgatat gtttgagaca
     3121 atgtacgaac acgaaggtat tggcttagct gcaccgcaag tgggcgtact taagcgtgtt
     3181 attactattg atattgaggg cgacaaaacc aatcaagtgg tgttaattaa ccctgaaatt
     3241 ttagaaagca gcggtgaaac cggcattgaa gagggctgtc tctccattcc cgggcatcgt
     3301 gccttagtgc cacgcaaaga gaaagtgaaa gtcaaagccc ttaaccgcca aggcgaagaa
     3361 attatcatcg atgccgatga tttatttgcg atttgtattc aacacgaaat cgaccattta
     3421 aacggcgtgc tttttgttga ccacatatcc aatttaaaac gccaacgcat caaagaaaaa
     3481 atgcaaaaat tgaaaaagca aattgcacga gcgaaataac caggcacaag cggttaaaat
     3541 ggcttaaaaa tttgcaaatt aagtgtgatg acacgataat aaccacagtt caactttaga
     3601 aaggataaaa atatgattat cgtcaccggc ggcgcaggta tgattggcag taacattgtt
     3661 aaagccttaa atgaaatcgg tcgcaaagat attttagtag tagataacct caaaaacggg
     3721 gaaaaattta tcaacttagt ggacttagat attgcggatt attgcgataa agaggatttc
     3781 atcgcctcta ttatcgccgg cgatgatttt ggtgagattg atgcggtttt ccatcaaggg
     3841 gcttgctcgg caaccaccga atgggacggc aaatacttaa tgcaaaataa ctacgaatat
     3901 tcaaaagagt tattgcattt ttgcttagat cgccaaattc ctttcttcta tgcctcaagt
     3961 gcggcaacct atggcggacg ttcggataat tttatcgaga aacgggagtt tgaaggtcca
     4021 ttaaatgctt atggctactc caaattcctg tttgatgaat atgtccgccg tattttgcct
     4081 gaggcagaat caccggtctg tggtttcaaa tatttcaacg tatacgggcc tcgtgagcaa
     4141 cacaaaggtt caatggcaag cgtggcattc cacttaaaca accagatgtt aaaaggggaa
     4201 aatccaaaac tatttgccgg ctcagagcag ttcttgcgtg attttgttta tgtggaagat
     4261 gtggcaaaag tgaatctttg ggcgtggcaa aacagcattt ctggcatttt caacctcggc
     4321 accggaaaag cggaatcttt ccaagaagtt gcgaatgcgg ttatcaaata ccacggcaaa
     4381 ggcgaaattg aaaccatccc attcccggat catttaaaat cccgctacca aacgttcact
     4441 caagcggact taaccaacct aagagccgct ggctacacag gcacatttaa aaccgttgct
     4501 gaaggaacag cggaatatat ggcgtggttg aatcgctaat caaacgttgt taaaaaagca
     4561 gaaagagcta cgaaatgtag ctctttttgt tataaggtta taaggattag aatgctgtcg
     4621 tatcttggaa taagcctact tttaaatcag tagcagtata gatcactcgc ccgtccactt
     4681 cgacttcacc atcagcaacc cccatcacta acttacgatt gaccacacgt ttcatatgga
     4741 tacggtaagt aacttttttc gctgtcggta aaatttgtcc ggtgaatttt acttcaccta
     4801 cgcccaatgc acgccctttg ccttcgccac caatccagcc gagataaaaa ccaaccagtt
     4861 gccacatcgc atctaagcct aagcaacccg gcattacagg atcgttaata aaatggcagt
     4921 taaaaaacca taaatccggt ttaatatcaa actcagcttc aatataccct ttttcaaaat
     4981 tgcctttttc ttggttcatt tcaatgatgc gatccatcat taacatcggt ggagccggca
     5041 attgaggccc tttttcaccg aataattctc cttttcctga tagcaaaagc tcttcgtaag
     5101 tatagcttgg tttgataatt ggtgtacaac tgttcattat ttttaaaatc ctgtaaagtg
     5161 aaaaaagcct gttatatcaa taactgataa atcaggctga tagtaacaga cttttaattc
     5221 agtgaacagt tgtaagctga cttgtcggat cagtctagct ttatcttttt gcaaattttg
     5281 cttaaaaatg aaccgcttgt ttcttcactt tcccgctcat ctagattctg gtgaatcaag
     5341 gtaaataatg ctgtttgggc tgagttaggg gctttctcat catcataaaa atcccgctca
     5401 aacagaatcg ctatggcatc aaacacattt tccaccgtcc aaattttaaa tagaccttgc
     5461 tccactgcct caacaacttc tgaacttaaa ctcaaatggc taacacaggc cgccggaata
     5521 atcacacctt gtttccccgt taattggcga gcgtgacaaa tattaaagaa gccttcaatt
     5581 ttctgattta ccccgcccac gcttagcaca ttcccaaatt gatcgatagc acctgtcacc
     5641 gcaatagatt gtggcaagcc aactttagct aaggcactca ctagtacatt gaaaatcgca
     5701 agagaagaac tatccccatc aatttcacca taggattgtt caaaagcaat cgaagctgaa
     5761 aacggtaact gggttggtag ttctaacaga ttcgctaaac aagattgagc gataataata
     5821 cctttggagt ggatatttcc ccctagctcc actttacgct caatatctgt aatttcgcca
     5881 tcaccatatt gtacattaca actcatgcgt aagggctcgc caaaactatg cggaatgcct
     5941 tcaaactcaa tgaccgataa gccattgatc tgcccaattt cctctccttc ggtttcaata
     6001 taaagttgat tagtcagaat atcacccacc gtatattgat ttaatatcga tgattgctgt
     6061 tctaaataat caaaataacc ttttacctcg tcaatttcgg tcacatttgg gtaaaaattt
     6121 tgaattccta ataaatgttt tctcaataat gtaggggaaa tagacaccaa ctctcggctt
     6181 tcactttcac gtatataggc ctgtaaaaat tgattaagcg ctgtggaagt taatcgctta
     6241 ttgatgagat tctcagcata attctgaaca taacctcccc actgctccac attatccgat
     6301 aatgataagt aggattttat ttcggtatat tgcccaaatt ggtacaaccc atcatcataa
     6361 gcagataaag tagaaatatc ttctctttcc cctactaaaa tcaatttaaa tgttgatttc
     6421 tctttagccg ataaataggg tgagtgatta acagaatttg gctcatattc accaaataat
     6481 aatgcctgtt ttagcttatc ccattgggta atatccaata agagcgaacg gatattaaca
     6541 attaaaattc ccttatctgc ctcgtgaatt gcacctttaa catattctgt tttcagctct
     6601 ctttccaaaa acacagaata accaaataga ctatttttat tgaaatccat tttgctaata
     6661 atcggcagat ctacactata ttttttcaga tattccgtga tttcattgat aaattccgga
     6721 aaaagctcag attttaagac taataaagca ccaaactgag attgtaaaaa ttgatttaac
     6781 gcttgttttg cttgaggttg aaaatctaaa aaattcaatt tctcatctaa acgagagaaa
     6841 tcattttgca ttactaattt ttggctacta agtaaagaaa gggtcacaaa tctgcctcat
     6901 tattgaaaat ttggtttatt atacaaaatt ccgctatact tgctaaatat agcttacttt
     6961 ttcctatcat attcagatta tgcgatatca gaaactcatc acccaagaaa caacttggaa
     7021 atggcaatat ctcctaaaaa aacatcgtga aggagaaaat attactaagt acgaagaaca
     7081 aagcttgatc gatttaaaag ttcagttcct ctctactttg caaaactctc cggacgaagt
     7141 ggaaaaatgg attaagtcgg aaatgacgcc tgagcaacgt aagaaaatgc gtcaatcagt
     7201 acgcgccaaa cgcaaacgct tctttaatgc tgaaaagcaa acaaccaaga aaaaatccat
     7261 tgatttagag tattcgagct ggttaaggct ctctaaatat gccaaaagcc aaaaactaac
     7321 gctttctgaa gctattgtca aattaaccga tgaactcgaa aataaacaac tttacttaga
     7381 gcaagttgct aaaatgaaat caagtttaaa agatttactg aaataaaaaa agaaaaaagc
     7441 ccactaacaa gcgggctttt tttgattcaa tttttcaact gaataactat attacatagt
     7501 tacttctttt gaaccttgaa cttggatttc aacacgacga tctggtgcta agcaagcgat
     7561 taatgcttta cgacctttaa ctgcatcaca tgtgtgacca gttactgggt ttgcttcacc
     7621 gtaacctaca gctgttacat tagctgggtt agtaccttta gaaacgatgt aattagctac
     7681 tgtttcagca cgacgttgtg aaagttttaa gttagatgct tctttaccga tgcggtctgt
     7741 gtaaccatta acttggatag ctaggtttgc taagcctaag ttgctgattt cagcgtgtgc
     7801 tgcatctaaa gaagttgctg ctgctggttt taagcttgat ttaccgaaat caaataaaac
     7861 atcagagctg aatgcgaagt ttttagttac aacttctggt gcttcaactg gtgctgcacc
     7921 ttgaccgaaa cggtatgata aacctgcaga tactgagtgg atatctgggc tgtattggaa
     7981 atttgtacct cttggtatta gataaccaac tgttgtacga actgccttat ctaagttacc
     8041 aacacgattt aagtattgat attcaacacg agcagctaat tcaggagtaa ttgcatactc
     8101 aagaccagca cctaataata gagatggttt taagttgtga actttaatgc gtgaatcttt
     8161 atctacatgt tgttgtacgt agtaatcgtt gcgaactaat gcaacaccta ctttaccgta
     8221 aacatctaag ttaggaacaa cttcgtagct tggttttaag cttaagtgcg caccgtgagc
     8281 tgaatgttta gccgcagttt tttctttatc atcaccagaa aatgtagtgt taccacgaac
     8341 acgaccaaag tagtcataac ctaattctgt cgctaaacca aagttgtttt ggtttaagat
     8401 ttggtaacca ccgaatacac cgtaagttac agagttacgg ttaataccga ggttaatacc
     8461 gaggttaata ccggatccat aatcatatgt gttgtcaaat tgagttaaac cgtcgtggaa
     8521 tgatgcccaa ccagctttag cacctgcgta gaaagtgtta gcttgtggag ctgcttgagc
     8581 tactgcagct gctgataata ctgctaatgc
//
LOCUS       DANLZC010000108         8541 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.108, whole genome shotgun sequence.
ACCESSION   DANLZC010000108 DANLZC010000000
VERSION     DANLZC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.108"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            79..3678
                     /gene="putA"
                     /locus_tag="PXE44_001918"
     CDS             79..3678
                     /gene="putA"
                     /locus_tag="PXE44_001918"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HDL5589401.1"
                     /translation="MMSYQLLPNTTIRPELSRHYRFDEQTLVQNLIEQAHCDEQADNI
                     KKLTTQLVGKVRTARQKASGVDALMHEFSLSSQEGVALMCLAEALLRIPDTATADKLI
                     RDKIAKGDWRSHTGNSPSLFVNAAAWGLVITGKLVSTHSESSLSSALSRLIAKGGEPL
                     IRKGVDVAMRMLGKKFVTGETIQQAIKNGEKRFAMGYRYSYDMLGEAALTAEDAKRYY
                     NDYVASIHAVGATSKGLGVYKSSGVSVKLSAIHPRYSLSQHQRVMDELYPRLKELFLL
                     AKKYDIGLNIDAEEADRLELSLDLMDRLLADPDLAGFNGIGFVVQAYQKRCPYVIEHL
                     IETARKHNRQLMIRLVKGAYWDTEIKRAQMDGAEGYPVFSRKVHTDLNYIVCAKKLLA
                     AQDVIYPQFATHNAQTLATIYHLAQGKRFEFQCLHGMGESLYDQVVGSDNLNVQCRIY
                     APVGTYQTLLAYLVRRLLENGANSSFVSQIVDENLSVDDLAQAPVQKAELTNGTMHPK
                     IPLPVDLFDDNRVNSKGYDLTDAVSLQNLENALNELTKQHYRAEPQLANDNTSGQESR
                     KIYNPANQAELVGEVIDATENDMEQAFESAAAFQSEWANTSPAVRAECLEKMADLMEA
                     NMPQLFDLAVREAGKTLNNAIAEVREAVDFCRYYAKEVRNNIEAYKNPRGIVVAISPW
                     NFPLAIFVGEVSSALVAGNVVLAKPAEQTSLMAHYAVSLFYQAGVPKAALQCLPGRGK
                     TVGVALVADHRVNGVIFTGSTETAKNINRNLHKNEKIIPLIAETGGQNAMIVDSSALG
                     EQVVIDVLNSAFDSAGQRCSALRVLYLQRDVADHILTMLKGAMAELKVGLPQHLTTDV
                     GPVIDKVAQQRLLDHIENLKKSAKSYYQVAISSEIAENGIFVPPTLLEIDHISQLKHE
                     VFGPVLHVIRFDADKLDQVIADINSTGFGLTSGLHSRIDETVDLWLKSVHAGNLYVNR
                     NTVGAVVGVQPFGGMGLSGTGPKAGGPLYLQRLVNRQEWTLNGLQGNAQAVDFDLNFA
                     IRKVLSGAEQQTALDLAKSLARRSPLGKTYPMQGITGEKNEMIFVARPQIGISKGNLA
                     SQVQALIAIVTVGSQAVAEKSSELAKHASAFGKKLQVVDDFNSLDHLSTLIVLDLLSS
                     EEKLHHAERKGAILTYIEGLDLALSLLPLVHEKAISINTAAAGGNASLMSEMD"
     gene            3787..4437
                     /locus_tag="PXE44_001919"
     CDS             3787..4437
                     /locus_tag="PXE44_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha3 family transposase"
                     /protein_id="HDL5589402.1"
                     /translation="MRKSRLSQYKQHKLIELFVAGVIARTAAELVNVNKTTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEIDESYFGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQSATLLPIIREKVKPDSLVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFWSQAKRHLRKFNGIPKAHFELYLKECEWRFNHSDLKSQIFLLKQLVKGGLG"
     gene            complement(4438..5325)
                     /locus_tag="PXE44_001920"
     CDS             complement(4438..5325)
                     /locus_tag="PXE44_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide transport protein"
                     /protein_id="HDL5589403.1"
                     /translation="MNKLTQLALISLAFTGSAFAAPKSFTYCLESSPAALNPQIATDG
                     ATLDVGQAIYNRLIDFEKGTTNLTPSLAERWEISEGGKIYTFFLRQNVQFQSNKSFTP
                     SRYFNADDVIFSINRQFDKTNPYHKLSGGNYEFFYGMNMQNIIKNVTKVDDYTVKIEL
                     SEPNAPFLANLAMDFMAIFSAEYANKMQIAGTPEKLDLEPIGTGAFQFYGYQKDAAVR
                     FTAFEHYWEGKAKIDRLVFSITPDASVRLAKLEKNECQAMPYPNPSDLSALKANKDIQ
                     LFEKSGLNIGYITFRGCPR"
     misc_feature    complement(5351..5496)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(5673..6683)
                     /gene="mglC"
                     /locus_tag="PXE44_001921"
     CDS             complement(5673..6683)
                     /gene="mglC"
                     /locus_tag="PXE44_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDL5589404.1"
                     /translation="MSALQQNRTLDFFKQNAIYFVLLILLIVIIVQDPSFLSLRNFSN
                     ILSQSSVRLIIALGVAGLIVTQGTDLSAGRQVGLAAVISATLLQSMENMNKVFPNLSE
                     IPIPVVVLTVCAIGAVIGLFNGFVVAYLNVTPFIATMGTMIIVYGINSLYYDAVGSSP
                     IAGFSDDFSTFAQGFFRFGDFRLSYITIYAVIATIIMWTLWNKTRFGKNVFAIGGNPE
                     AARVSGVNVPLNLMGIYMLSGIFYAFGGMLEAGRIGSATNNLGFMYEMDAIAACVVGG
                     VSFAGGVGTIIGVVTGVLIFTVINYGLTYIGVNPYWQYIIKGGIIILAVAIDSLKYAK
                     KK"
     gene            complement(6697..8196)
                     /gene="mglA"
                     /locus_tag="PXE44_001922"
     CDS             complement(6697..8196)
                     /gene="mglA"
                     /locus_tag="PXE44_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDL5589405.1"
                     /translation="MTGEVLLTMTNVSKSFPGVKALDNANLTVKSHSVHALMGENGAG
                     KSTLLKCLFGIYAKDEGDILFLGKPVNFKTSKEALENGISMVHQELNLVKQVTVMDNL
                     WLGRYPLKGPFVDHDKMYRDTKAIFDELDIDVDPRDKVAKLSVSQMQMIEIAKAFSYN
                     AKIVIMDEPTSSLSEKEVEHLFKIIQKLKDRGCGIIYISHKMDEIFKICDEITILRDG
                     KWVNTVAIKDTSMDQVVSMMVGRELTQRFPEKTNLPKEVILEVEHLTAKNQPSIQDVS
                     FSLRRGEILGIAGLVGAKRTDIVETIFGVREKAGGTIRLNDKEINNRNAFDAINNGFA
                     LVTEERRSTGIYANLSIEFNSLISNMKSYLGKFGLLSNKKMKSDTQWVIDSMNVKTPS
                     HQTHIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDIGAKFEIYQLILELANKDK
                     GIIMISSEMPELLGVTDRILVMSNGKVAGIVETAHTSQEEILQLAAKYL"
     gene            complement(8268..>8541)
                     /locus_tag="PXE44_001923"
     CDS             complement(8268..>8541)
                     /locus_tag="PXE44_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5589406.1"
                     /translation="ALEATKAHGRKLPIFGVDALPEALQLIKKGELAGTVLNDGVNQG
                     KAVVQLSANLADGKDAVAGTQWKLENRVVRIPYVGVDKDNLSEFLK"
BASE COUNT         2491 a         1862 c         1834 g         2354 t
ORIGIN      
        1 aataacaagc ggtcggtttt atcttaaaat ttgcaagttt atacataaat ttgatcgctt
       61 gctgtataga aaggaaaaat gatgtcatac caacttttac ctaacaccac tatccgtcct
      121 gaattgagcc gtcattaccg ctttgatgag caaaccctgg tgcaaaatct gatagaacaa
      181 gcacattgtg atgaacaggc tgataacatt aaaaagctca ctacccaatt agtcggtaaa
      241 gtccgaactg cacgccagaa agcaagcggg gttgatgcgt taatgcacga attttcactc
      301 tccagccaag agggggtggc gttgatgtgc cttgccgaag cattactacg cattcctgac
      361 accgccactg cggataaatt aattcgagac aaaattgcca aaggggattg gcgttcacac
      421 accggcaaca gcccctctct gtttgtgaat gcagcggcgt ggggcttggt gattaccggc
      481 aaattggttt caacccacag cgaaagctca ctctcatcag cactttcacg cctgatcgcc
      541 aaaggcggag aaccgctgat ccgcaaaggt gtggacgtag caatgcgtat gctcggcaag
      601 aaatttgtaa cgggcgaaac cattcaacag gcgattaaaa acggcgaaaa acgcttcgca
      661 atgggttatc gttacagcta cgatatgctg ggcgaagcgg cactcaccgc cgaagatgcc
      721 aaacgctatt acaacgacta tgttgcctct attcacgctg tgggtgccac ctcgaaaggc
      781 ttgggcgtgt ataaatcctc gggtgtgtcg gtcaaactct ccgccattca ccctcgttat
      841 tccctttccc aacaccaacg tgtgatggac gaactctatc cacgtctgaa agaactgttc
      901 ttgctggcga aaaagtatga catcggcttg aacattgatg ccgaagaagc cgaccgcctc
      961 gaattgtcgc ttgatttgat ggacagactg cttgccgacc ctgatttggc agggtttaac
     1021 ggcattggtt ttgtggtgca ggcgtaccaa aaacgttgcc cgtatgtgat cgagcattta
     1081 attgagaccg cccgtaaaca taatcgccag ctaatgattc gcttggtaaa aggcgcatat
     1141 tgggacactg aaatcaagcg ggcgcaaatg gacggtgcag agggctatcc ggtcttctcc
     1201 cgtaaggtgc ataccgattt gaactacatc gtttgtgcca aaaaattgct ggcggcgcaa
     1261 gatgtgatct atccgcaatt tgccacccac aacgctcaaa ctctcgccac catttaccac
     1321 ttggcacagg gcaaacgttt tgagtttcaa tgcttgcacg gtatgggaga gagcttgtac
     1381 gaccaagtgg ttggcagcga taatcttaac gtgcaatgtc gcatttatgc ccctgtcggt
     1441 acttatcaaa ccctgcttgc ctatttagta cgtcgcttgt tggaaaacgg agcgaacagc
     1501 tcatttgtta gccaaattgt ggatgaaaat ctcagtgtag atgatctggc tcaagcccct
     1561 gttcaaaaag cagaactgac caatggcact atgcacccga aaattccatt gccagtcgat
     1621 ttatttgacg acaatcgtgt taattccaaa ggctatgatt taactgatgc agtaagccta
     1681 caaaatttag aaaatgccct caatgaactt accaaacagc attaccgtgc tgaaccacag
     1741 ctggcaaatg ataatacaag cggtcaagaa agccgaaaaa tttacaatcc agcgaaccaa
     1801 gctgaattag tcggcgaagt gattgatgcc accgaaaacg atatggaaca ggcgtttgaa
     1861 agtgcggcgg cgttccaaag tgaatgggcg aatacttccc ctgcggtgcg tgccgagtgc
     1921 ttagaaaaaa tggcggactt gatggaagcc aatatgccac aactgttcga tttggcggtg
     1981 cgtgaggcag gcaaaacgct caacaatgca attgctgaag tgcgtgaagc cgtcgatttc
     2041 tgtcgttatt atgccaaaga ggtacgcaac aacattgaag catacaaaaa tccgcgtggc
     2101 attgtagtag caattagccc gtggaacttt ccgttagcga tttttgtggg tgaagtttca
     2161 tctgcgttgg tggcaggcaa tgtggtacta gcaaaaccag ctgaacaaac ttcgttaatg
     2221 gcacattatg ctgtcagcct tttctaccaa gcaggtgtgc cgaaagcggc gttgcaatgc
     2281 ttaccgggta gaggaaaaac ggttggggtg gcgttggtgg cagatcatcg tgtgaacggc
     2341 gtgattttta ctggttcaac agagacagct aaaaacatta accgcaattt gcacaaaaac
     2401 gagaaaatta taccgcttat tgccgaaact ggcggacaaa atgcaatgat tgttgatagc
     2461 tcggcactgg gcgagcaggt tgtgatagat gttttaaact ccgcatttga ctcagcaggg
     2521 caacgttgtt cggcattgcg tgtgctgtat ctgcaacgtg atgttgccga tcacattctc
     2581 acaatgctca aaggagcaat ggcggagttg aaagttggtc ttccacaaca cttaactacc
     2641 gatgtcggac cagtaatcga taaagtcgca caacaacgct tgctcgatca tattgaaaat
     2701 ctcaaaaaat cggcgaaaag ttactatcag gtggctatct cctctgaaat tgccgaaaat
     2761 ggtattttcg taccgccaac gctgttagaa atagatcata tcagccagct taaacacgaa
     2821 gtattcggac cggtgctaca cgttatccgt tttgatgcgg ataaactcga ccaagtgatt
     2881 gccgacataa actccacagg tttcggtcta accagcggtt tgcatagccg aattgatgaa
     2941 acggtggatt tatggttgaa atctgtccac gctggaaatc tgtatgtaaa ccgtaacacg
     3001 gtaggggctg tggtcggcgt acagccattc ggtggaatgg ggttatcagg aactgggccg
     3061 aaagctggcg ggccgctcta tctacaacgt ttagtaaacc gccaagaatg gacattgaac
     3121 ggtttacagg gcaatgcaca agcggtcgat tttgacttaa attttgcaat ccgcaaggtt
     3181 ctaagtggag ctgagcagca aacggcgtta gatctggcca aaagccttgc acgccgctcg
     3241 ccactcggaa aaacctaccc aatgcagggt attaccggcg agaaaaacga gatgattttc
     3301 gtggctcgcc cacagattgg gattagcaaa ggtaatttgg cttcacaggt gcaagccttg
     3361 attgccattg taacggtcgg cagccaagcg gtggcagaaa aatcaagcga gctggcaaaa
     3421 cacgcctctg cctttggtaa gaaattacag gtggtggatg atttcaacag tctcgatcac
     3481 ctcagcacac tcattgtgct tgacctgctt tctagtgagg aaaaattgca tcatgccgaa
     3541 cgcaaagggg cgattttgac ctacatcgaa gggctggatt tagcgctctc actgctgccg
     3601 ttggtacacg agaaagcaat cagcatcaac accgctgccg cagggggaaa tgccagctta
     3661 atgagtgaaa tggattaagc aacaaacaaa agagtataag agggcagatc acaatgatct
     3721 gcctttttat tttaaggggc tgtcctagat aacgactaaa acttccattt aggttaagat
     3781 aaccctatga gaaaaagtcg tctaagtcag tacaaacaac ataaactcat agagctgttt
     3841 gtagcaggcg taatcgctcg aacagccgca gagttagtga atgtaaataa aaccaccgca
     3901 gcctattatt ttcaccgttt gcgattgctc atctatcaac atagccctca tttggaaatg
     3961 tttgagggag aaattgagat tgatgagagc tatttcggcg gtcatcgcaa gggtaaacga
     4021 ggtcgtggtg cagcaggcaa aaccgccgta ttcgggcttt taaagcgtga cggcaaggtt
     4081 tataccgtag tagtgccgaa cacacaatct gctacacttt tgccgattat tcgggaaaag
     4141 gtgaagcccg atagccttgt ttacaccgat ttttatcgaa gctatgatgt gcttgatgtg
     4201 agtgaattta accattttcg tatcaatcac agcacacact tcgcagaaaa tcaaaatcac
     4261 attaacggaa ttgagaattt ttggagtcaa gcaaaacgtc atttacgcaa gtttaatggt
     4321 attccgaaag cccattttga gctatatttg aaagaatgcg aatggcgttt taatcacagt
     4381 gacttaaaat ctcaaatatt ccttttaaaa caattggtta aaggggggtt aggttagtta
     4441 tctaggacag cccctaaaag taatataacc gatatttagg cctgactttt caaataattg
     4501 aatatcttta ttcgctttta aagcagacaa atctgacggg ttcggataag gcatcgcttg
     4561 gcattcgttc ttctccaatt tagctaaacg tactgaggca tcgggcgtaa tactaaatac
     4621 aaggcgatct atcttcgctt ttccttccca atagtgttca aatgctgtaa atcttaccgc
     4681 tgcgtccttt tggtaaccat agaactggaa tgcacctgtg cctataggtt ctaaatctaa
     4741 tttttccggc gtacccgcta tttgcatttt atttgcatat tccgcagaaa agatcgccat
     4801 aaaatccata gctaaattag caagaaaggg ggcattcggt tcagataatt cgatttttac
     4861 cgtgtaatca tctacttttg ttacattttt gatgatgttt tgcatattca ttccgtagaa
     4921 aaactcatag ttaccgccgg acaatttgtg atatggattc gtcttatcaa actgacggtt
     4981 aatcgaaaaa ataacatcat cagcattaaa ataacgacta ggggtaaaac ttttattact
     5041 ctggaactgc acattctgac gcaagaaaaa agtataaatt tttccgcctt cactgatttc
     5101 ccatcgctct gccagagaag gagtgaggtt agtggtacct ttttcaaaat caatcaggcg
     5161 attatagatt gcttgcccga catcaagagt agctccatct gttgcaatct gaggattcaa
     5221 tgctgccgga gatgattcca aacagtaagt aaaagattta ggtgctgcga aagcagaacc
     5281 tgtaaatgct aaagaaataa gtgcaagttg tgtcagcttg ttcattgtca tatccttaaa
     5341 tgttaagcaa taaaaaaccc tttcaaaagt tgccttccga aagggttaga attgggtttg
     5401 cgattttgga ttgcgtttgc catttcaggt tttcggaaga tatcttccga acacgccaaa
     5461 tgcccgaaag attaatcggt caaatggtag tgatgatgtt taacgcaata gctcataata
     5521 ttctccaaaa ttgaatgttt aggaatttac gctttttaaa aatcaattgc aaacgttttc
     5581 tttaaaattt tgtaaaaaat tcaacatttt tcaccgcttg tatcgtacct aggagcaaat
     5641 ggcgatttgc tcctaggttt ccaagcaata ggctattttt tcttcgcata tttaagtgag
     5701 tcaatcgcta ccgccaagat aatgatgcca cctttaataa tatattgcca gtaagggttt
     5761 actccaatat aggttaagcc gtagttgata acggtgaaga ttaacacccc tgttactaca
     5821 ccgatgatag tacccacacc accggcaaag gataccccac ctaccacaca agcggcaatg
     5881 gcatccattt catacataaa gcctaagttg ttggttgcac taccgatacg acctgcttct
     5941 aacataccgc cgaaggcata gaagatgcct gaaagcatat aaatacccat caggttaagc
     6001 ggtacgttta caccggaaac tcgtgctgct tcagggttgc cgccaatagc aaatacgttt
     6061 ttaccgaagc gggttttatt ccataacgtc cacataataa tggtagcaat cacggcataa
     6121 atagtgatgt aggatagcct aaagtcgcca aagcggaaga agccttgtgc aaaggttgag
     6181 aaatcatcgc taaagcctgc aattggcgat gagcctactg catcgtagta aagcgagttg
     6241 ataccataca caataatcat tgttcccatc gtggcaataa acggtgttac gttgaggtaa
     6301 gccaccacaa agccgttaaa taaaccaatg acagcaccga tggcacacac ggttaataca
     6361 accacaggaa tcgggatttc cgagaggttt gggaatacct tgttcatatt ttccattgat
     6421 tggagcaagg tggcggaaat taccgctgcc aagcccactt ggcgaccggc agataagtct
     6481 gtcccttgtg ttacaatcaa gcctgcaacc cctaatgcaa taatcaaacg cacagaagat
     6541 tgcgataaaa tgttactgaa gttacgcaag ctcaagaaac ttgggtcttg cacaataatc
     6601 acaataagta agattaataa tacgaagtaa atcgcatttt gtttaaaaaa atcgagtgtt
     6661 ctattttgtt gtaaagcaga catacttcac tccttgttat aaatattttg ctgcgagttg
     6721 caagatttct tcttgcgagg tgtgagcggt ttcgacaata cctgcaactt tcccgttact
     6781 catcactaaa attcggtctg tgaccccaag caattccggc atttccgatg aaatcataat
     6841 gatgccttta tctttattcg ccagctctaa aatcaactgg taaatttcaa attttgcccc
     6901 gatatcgata ccacgggtcg gctcgtcaag catcaagatt tcaggctggg ttaacaacca
     6961 acgcccgata atcacttttt gttggttacc gccggaaagt gagccaatat gcgtttgatg
     7021 cgatggggtt tttacattca tcgaatcaat tacccattgg gtatcgcttt tcattttttt
     7081 attgctcagt aagccgaact tgcctagata agacttcata tttgaaatca gagagttaaa
     7141 ctcaatgctt aagttggcat aaatccctgt tgaacggcgt tcttctgtaa ccaatgcaaa
     7201 gccgttatta atcgcatcaa aagcgttgcg gttattgatt tctttatcgt ttagtcggat
     7261 agtgccgcct gctttctccc gtacaccgaa aatggtttca acaatatcag tccgttttgc
     7321 tccaactaag ccggcgatgc ctaaaatttc ccctctgcgt aagctaaagc tgacatcttg
     7381 aatagagggt tgattttttg ccgttaaatg ctcaacttca agaatcactt ctttcggcag
     7441 attggttttt tcagggaaac gctgggtcag ctcacgccct accatcattg aaaccacttg
     7501 atccattgag gtatctttaa ttgcaacggt gtttacccat ttaccgtcac gcaaaatggt
     7561 aatttcatca caaattttaa aaatttcgtc cattttgtgt gaaatataga taatgccaca
     7621 gcctctgtct ttcagttttt ggataatttt aaataaatgc tcgacttctt tttctgaaag
     7681 tgacgaagtt ggttcgtcca taatcacaat tttggcattg taagagaaag cttttgcaat
     7741 ctcgatcatc tgcatttgtg aaaccgataa tttggctact ttatcacgtg gatcaacatc
     7801 aatatctaat tcatcaaaaa tcgccttagt atcacgatac attttatcgt gatctacaaa
     7861 aggccctttt agtgggtaac gacctagcca taagttatcc attaccgtca cttgttttac
     7921 taggttcaac tcttggtgaa ccattgagat cccattttct aacgcctctt ttgaggtctt
     7981 aaaattcacc ggcttgccaa gaaagagaat atctccctca tctttagcat aaatcccaaa
     8041 taagcatttc agtaacgttg atttacctgc tccattctct cccattaagg cgtgaacact
     8101 atgggatttc accgttaagt tagcattatc taaggctttt accccgggaa atgacttgct
     8161 aacatttgtc attgtgagta aaacttcacc tgtcatatct cacccctgat tacatcgaaa
     8221 aatcgaaaaa aagcacgggt atccatcacc cgtgctaaaa gcgtgaatta ttttaaaaat
     8281 tcagataagt tgtctttatc gacaccaacg taaggaatac gcaccacacg gttttccaat
     8341 ttccattgtg taccagcaac tgcatcttta ccgtctgcta agttagcaga aagctgaaca
     8401 accgctttac cttggtttac accatcattt aataccgtac cggctaattc accttttttg
     8461 attaattgaa gtgcttctgg caatgcatct acaccgaaga ttggtaattt tctaccgtga
     8521 gctttggttg cttctaatgc a
//
LOCUS       DANLZC010000109         8461 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.109, whole genome shotgun sequence.
ACCESSION   DANLZC010000109 DANLZC010000000
VERSION     DANLZC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.109"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            24..1829
                     /locus_tag="PXE44_001924"
     CDS             24..1829
                     /locus_tag="PXE44_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDL5589407.1"
                     /translation="MLSKLQRFTLMIKSTIIKHPLEILFVTYVTILLINNVSDYELIK
                     RFQNLIALFPICFILLYLIRDTKAYWLFAPIPIAIVFFFGQINLTLLEDSRYWATIFC
                     VFLCLISQYWYKNNHYFVAGTTNKLVNIVFATVLSGIIFATLSAITIAIIALFDLNKQ
                     NNFHIFEKFGIFSSFWAFPVLFLTLEQQTTNDSRYLNRISEMLLNWILSPALIIYTAI
                     IYAYVIFIAAKGQMPDGVVANVALPYLAFGLAIQAVQLLLKNAKWQWFYSHFTYLALL
                     PLTLIWYAIYIRFSHYGLTEARIYLVIGTITLSICYTLLLFKQLAQYRLFSAVSIAII
                     LISVFILDPKTVALKDQTKRLDNYLLKHKLLDVNNKISTKAVLKYQESLNENRDEAEY
                     FYSMLHYVARGMPAAQFKEKYGVNSSYDLMSEIRERNTDPRYFEATNSELVRLTKADM
                     ANAREYITFSSQLYRPNSFSHQKIENKLCQKLLSQGYYFEGNILNIEYQYSETAQDCS
                     SITQDPTEFVIYFKGLGTTIEFNINDILKKIFAKHQLSITQQHKNEVLEKIKGDLLKM
                     DLGNSVLGLKHIELRFEDNIGYIVESISTQYIMLK"
     gene            complement(1872..4901)
                     /locus_tag="PXE44_001925"
     CDS             complement(1872..4901)
                     /locus_tag="PXE44_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HDL5589408.1"
                     /translation="MSLPNYYQDPSVLHLNTTLHHAYFIPFAKGQTPNQLEREQSELF
                     TLLNGDWKFNFYPSPHDLPEDFLNTKFTHQIPVPSNWQTQGFDAHQYTNINYPIPFDP
                     PYVPHQNPCGVYQREITFNPKPNKRYLLNFEGVDSCLFVYVNGEFVGYGQISHSTNEF
                     DITKQLKQGKNSLVVVVLKWCDGSYLEDQDKFRMSGIFRDVYILEREQNYLQDFFIRY
                     QLNADLSKAELKVETQFSTYTEAVHFQLFDPENRLVCEQTSGDFCQEICNLCLWNAED
                     PTLYTLCLSYQNEIIEQKIGFRQIRVENGVMLFNNAPIKFKGVNRHDSDPKTGYSISR
                     EQALVDLRLMKAHNINAIRTAHYPNAPWFSELCDQLGFYLIAESDLESHGSSAVYIES
                     PENSIFLNVPNPNKQEEIRQQAVDNFCYFARDPLFKQAILDRNIANVERDKNRTSVII
                     WSLGNESGFGENFEVAARWVKQRDPSRLTHYEGSIYQHSAHQNDLSNLDLYSEMYPST
                     ERLEEYFSDSANHKPFILCEYSHAMGNSSGDMEDYWQTFQRYSGACGGFVWEWCDHAP
                     LLPNSELSAEQKTEKYGYGGDFGETLHDGNFCMDGLVSQQRVPHSNLLEVKNVNRPVR
                     AELKAGKIWLKNQLDFSDLADYLTVHYCFSENGETTLQGQCLVNCQPNQTACLPLTLP
                     EPNGKLQLLTLDYSLNESTALLEKGHHLGFDQITLHQAMVTPALNPRPQAVIFVQDFA
                     NEWRIQQGDYRYTFDKLKGIFSRIEKNGEAVIEQPLDFNIWRAPTDNDRLIREFWKKA
                     GYDNCYTRAYHTELMQDKNGVNITAECGIVATAQSRILTLKVNYHLAPNGEMTIQVQA
                     TRPAHLPFLPRFGLRFFLNKRNNQVSYFGYGEQESYIDKYHLAKLGAYQTSARENHTD
                     YLKPQENGSHFGTHYVQANGITVTSPQPFSFNLSPYTQEELTTKSHSYDLVESPSTVL
                     CVDYKMSGIGSNSCGPNLKPQYRLNETNFEWLLKLSF"
     gene            5079..5936
                     /gene="menB"
                     /locus_tag="PXE44_001926"
     CDS             5079..5936
                     /gene="menB"
                     /locus_tag="PXE44_001926"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005723289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDL5589409.1"
                     /translation="MLYPSEEFLYAPVEWVDHSEGYTDIRYHKSKDGIAKITINRPEV
                     RNAFRPQTVKEMIEAFSDARFDEKIGVIVLTGEGEYAFCAGGDQKVRGDYGGYKDESG
                     VHHLNVLDFQRDIRTCPKPVVAMVAGYAIGGGHVLHMLCDLTIAADNAKFGQTGPKVG
                     SFDGGWGASYMARLVGQKKAREIWFLCRQYNAQEALDMGLINTVVPYADLEKETVRWC
                     REMLQNSPIALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNEKREP
                     DFSKFRRNP"
     gene            6019..8421
                     /gene="mrcB"
                     /locus_tag="PXE44_001927"
     CDS             6019..8421
                     /gene="mrcB"
                     /locus_tag="PXE44_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HDL5589410.1"
                     /translation="MSDVELNEQSEQEPEQKPKKRNYFLSTLFKIGVIGACSMAFYGI
                     YLDGKIRSKMDGQIWELPAEVYARIESIKIEDNLTLEQTKQALLDNGYRQVSQVATPG
                     DFKIEENQLVLLRRAFPFPETPESQRVLRLTFKQDKLTYIEDLVQGKLIEEFRLDPKL
                     LALLHSDSDEERQALRLQQYPRLLIESLILTEDRRFYQHDGISPIGIARALITNYQAG
                     RTVQGGSTLTQQLVKNLFLSNERSLVRKVNEALMSVILDFRYDKNRILETYLNEIYLG
                     QNGSYQIHGFALASQFYFGRPIQEISLDQIALLVGMVKGPSLYNPWRNPQGAVERRNV
                     VLRLLLDHQVINQELYALLSSRPLNIREKGTIYREQPAFMQALTIDLKNELGEIKSAQ
                     LSGTKIFTTLDRKQQRSAEQAVITGLEDLESKNKKVSDLQSAIVVAEYRTGKVRAIVG
                     DRLTQFAGFNRAIQSKRQIGSLVKPSVYAIALAQPELFRLNTPIQNKQISIKVGSTYW
                     TPRNYDRSFSGSVMLMDALVRSLNIPTVNIGMKVGLKKVIAKQKEMGWDNAEIPPYPS
                     TLLGSYSISPYDVTRSYQVIANEGLKTPLTTIEAIISHNGEMFYQRDSGKVSEQVLPP
                     EAAVQTMYAMQQVVERGTARSLQNDFAKLKLAGKTGTTNNARDTWFVGIDGEHVTTVW
                     LGKDNNGDTNLTGSSGALYVYKQYLERALPQAYRLPKLNTMQWVGINSYGSWNCDSGR
                     QIPVWRDKGQRYCTSAGNVVATEKPSVWDALSLKKENIEAKAEEAEGKQPIPTEEVAP
                     IE"
BASE COUNT         2494 a         1691 c         1825 g         2451 t
ORIGIN      
        1 ttttcatagg caggagatct attatgctaa gtaagctaca acgctttacc ttaatgataa
       61 aaagcaccat cattaagcat ccactggaaa ttttattcgt tacttacgtc acgattttac
      121 ttatcaataa tgtgagcgac tacgaattaa tcaaacgctt tcaaaatcta attgctcttt
      181 ttcctatttg ctttattttg ctttacctga tacgcgatac caaagcttat tggctattcg
      241 ctccgattcc gatagcaatc gtctttttct ttggtcaaat taatttaaca ttattggaag
      301 attctcgtta ttgggctacc atattttgtg tattcctttg tttgatcagt caatattggt
      361 acaaaaacaa ccattatttt gtagccggaa caactaataa attagttaac attgtctttg
      421 ctacagtttt atctggcatt atttttgcaa cattaagcgc tattaccata gccattattg
      481 ctttatttga tttaaataaa caaaacaact ttcatatttt tgaaaaattt ggcatttttt
      541 ctagtttttg ggcattccct gttttatttt taactcttga acaacagact accaatgatt
      601 ctagatatct aaaccgcatt agtgaaatgt tactaaactg gattttgtct ccggcattaa
      661 ttatctatac tgcaattatt tatgcttatg taatttttat tgcggctaag gggcaaatgc
      721 cagatggggt tgttgcaaac gtagcgcttc cctatttagc tttcggccta gctatacaag
      781 cggtgcaatt attactaaaa aatgcaaaat ggcagtggtt ttattctcac tttacctatt
      841 tagcactttt gccgttaaca ttaatttggt atgccatcta tattcgtttc agccattatg
      901 gcttaactga agcccgtatt tatttagtga ttggcacaat aaccttatct atttgctata
      961 ccttattact atttaaacag ttggcgcaat atcgtctctt ttctgcagtg tcgatagcaa
     1021 ttattttaat ttcagtgttt attttagatc cgaaaacagt tgcgcttaaa gatcaaacca
     1081 agcggttgga caactatttg ctaaaacaca aattattaga tgtaaataac aaaatcagca
     1141 caaaagctgt acttaaatac caagaatcat taaatgaaaa ccgagatgaa gccgaatatt
     1201 tttattcaat gctccactat gttgccagag gaatgcctgc tgcacaattt aaagagaaat
     1261 atggcgtaaa ttcaagctac gatttaatga gtgaaataag agaaagaaat actgacccac
     1321 gttattttga agctactaat tctgagctgg ttcgcttaac taaagcagat atggcaaatg
     1381 ctcgtgaata tattacattt agtagccaat tatatcggcc taattcattt tcccatcaaa
     1441 aaatagaaaa taagctatgc caaaaactcc tttctcaagg ttactatttt gagggaaata
     1501 tattaaatat tgagtatcaa tactcagaaa cggctcaaga ctgctcttca ataacacaag
     1561 atcctactga atttgttatc tactttaaag gacttgggac tactattgaa tttaacatta
     1621 acgatatttt gaaaaaaatc tttgctaaac atcagttatc tattacccag caacacaaaa
     1681 atgaggtttt agaaaagatt aaaggcgatt tactcaaaat ggatttagga aattcagtac
     1741 ttgggcttaa gcatattgag ttaagatttg aagacaatat cggctatata gtagaaagta
     1801 ttagtacaca atatattatg ctgaaataaa aggagatgct cgttcaatta aatttgaacg
     1861 agcatttttc gttaaaaact caatttaagc agccactcaa aattagtctc atttaaccga
     1921 tattgcggtt tcaaattcgg gccgcaagag tttgagccaa tgccgctcat tttgtaatcg
     1981 acacacagca cggtggaagg gctttctacc aaatcataac tgtggctttt agtggttagc
     2041 tcttcctgtg tgtaaggcga aagattaaaa ctaaacggct gtggagaagt aacagttatc
     2101 ccatttgctt gcacataatg cgtaccgaaa tggctgccgt tttcctgcgg tttgagataa
     2161 tcggtatgat tttctcgggc gctggtttga taagctccga gtttcgccaa atggtatttg
     2221 tcgatataac tctcctgctc gccatagcca aaatagctca cttggttatt ccgtttattc
     2281 aagaaaaacc gcaagccaaa gcgaggtaaa aacggcaaat gagcggggcg ggttgcctga
     2341 acctgaatcg tcatttcgcc attcggggca agatgataat tcactttcag ggttaaaatc
     2401 cgactttgag cggttgccac aatgccacat tcggcagtaa tgtttacgcc gtttttatct
     2461 tgcatcagtt cggtatgata cgccctggtg taacagttat cgtagccggc ttttttccaa
     2521 aactcacgaa ttaagcggtc gttatcggtt ggggctcgcc aaatattgaa atccaacggt
     2581 tgctcaatta ctgcctcgcc gtttttctca atccggctga aaatgccttt gagtttatcg
     2641 aaagtgtagc gatagtcgcc ctgttgaatc cgccattcat ttgcaaaatc ttgcacaaaa
     2701 atgaccgctt gcgggcgtgg attaagtgct ggagtgacca tcgcttgatg cagcgtgatt
     2761 tggtcaaagc ctaagtgatg ccctttttcc aacagagccg tgctttcatt taaagaataa
     2821 tcaagggtta aaagttgcaa tttgccgttt ggttcgggca gcgttagcgg caaacaagcg
     2881 gtctgatttg gctgacaatt taccaaacat tgtccctgca gggtggtttc gccgttttcg
     2941 ctgaaacagt aatgcacggt cagataatca gctaagtcgc tgaaatccaa ctgatttttc
     3001 agccaaattt tgcccgcttt taactcagct cgcaccgggc gattcacatt tttcacttct
     3061 aacaaattag agtgtggcac acgctgttgg gaaaccaagc cgtccataca gaaattgcca
     3121 tcgtgcaagg tttcgccgaa atcaccgcca tagccgtatt tttcggtctt ctgttcggca
     3181 cttagctcag agttggggag cagaggagca tggtcgcacc attcccacac aaagccaccg
     3241 cacgcaccgc tgtagcgttg gaaggtttgc caataatctt ccatatcacc actcgaattg
     3301 cccatcgcgt gagaatattc acacaaaata aaaggtttgt ggttggcaga gtcggaaaaa
     3361 tattcctcta accgctcggt gcttgggtac atctcgctgt ataaatcgag gtttgaaaga
     3421 tcgttttgat gtgccgaatg ttggtaaatc gaaccttcgt agtgagttaa acggcttgga
     3481 tcccgttgtt ttacccaacg ggctgcgact tcaaaatttt caccaaagcc ggattcgtta
     3541 ccaagtgacc aaataatgac cgaagtgcgg tttttgtctc gctccacatt ggcaatgttg
     3601 cgatctaaaa tcgcctgttt gaacagcgga tctcttgcaa aataacagaa attatcgacc
     3661 gcttgttggc gaatttcttc ctgtttgttc gggtttggca cattcaggaa aatgctgttt
     3721 tccggacttt caatatacac tgcactggag ccgtgagatt caagatcact ttcggcaatc
     3781 agataaaaac cgagctgatc gcacaattca ctaaaccacg gagcgttcgg gtaatgggcg
     3841 gtgcgaatag cgttgatgtt atgggctttc attaagcgta aatccaccaa cgcttgctca
     3901 cggctaatgc tgtagccggt tttcgggtcg gaatcgtggc ggtttacccc tttaaattta
     3961 atcggggcgt tgttgaatag catcacgcca ttttccacac gaatctgacg aaagccgatt
     4021 ttttgctcaa taatttcgtt ttggtagctc aagcatagcg tgtagagtgt cggatcttcc
     4081 gcattccaca aacacaaatt gcaaatttct tggcaaaaat caccgcttgt ttgctcgcag
     4141 actaagcggt tttcagggtc gaaaagttga aaatgcacag cttcggtgta ggtggaaaat
     4201 tgtgtttcca ccttgagttc tgcctttgaa agatcagcgt ttaactggta gcggataaag
     4261 aaatcttgca ggtagttttg ctcacgttcc aaaatataaa catcacggaa aatgcctgac
     4321 attcggaatt tgtcttgatc ttccaaataa ctgccgtcac accattttag caccaccacg
     4381 acgaggctgt ttttgccctg tttaagctgt ttggtgatgt caaattcatt ggtgctgtgg
     4441 ctgatttgcc cgtagccgac aaattcgccg tttacataaa caaacaggca cgaatctacg
     4501 ccctcaaaat ttagcaaata gcgtttgtta ggcttggggt taaacgtgat ttcacgctga
     4561 tacaccccgc aagggttttg gtgtggcaca taaggcggat caaacggaat cgggtagttg
     4621 atgttagtgt attgatgggc atcaaagcct tgagtttgcc agttagacgg cacggggatt
     4681 tggtgggtaa atttggtgtt gaggaaatcc tccggcagat cgtgcggact tggatagaaa
     4741 ttaaacttcc aatcgccatt tagcaaagta aataactcag attgttcccg ttccaactga
     4801 ttcggcgttt gccctttagc aaaaggaata aaataagcgt ggtgtagcgt ggtattaagg
     4861 tgcaacacac tcgggtcttg gtagtaattc ggtaacgaca taaaagccct atgatcaaaa
     4921 agatataaat acgaatacac tttaattctc ttataaaata gggcaatcaa ctatgtgaaa
     4981 aattgtgagg cggatcacaa aataatttcc tcaccttttt ccttcgattc cgttataatc
     5041 acaagaattt tttatgtcat ttttcaagga atttaccaat gttatatcca agcgaagaat
     5101 ttttatatgc accggttgag tgggttgatc acagcgaagg ttacaccgat attcgttacc
     5161 ataaatcaaa agacggtatc gctaaaatca ccattaatcg tccggaagtg cgtaatgctt
     5221 tccgtccgca aacggtaaaa gagatgattg aagccttttc tgatgcccgt tttgatgaaa
     5281 aaatcggcgt catcgtatta accggtgaag gcgaatatgc gttctgtgcc ggcggcgacc
     5341 aaaaagtgcg tggcgattac ggcggctaca aagatgaaag cggtgtgcat cacttaaatg
     5401 tgttggattt ccaacgtgat attcgtacct gcccgaaacc ggtggtggca atggttgccg
     5461 gctatgcgat tggtggtgga cacgttttac atatgttatg tgatttaacc attgccgctg
     5521 acaatgcaaa attcggtcaa accggcccga aagtggggtc atttgacggc ggttggggtg
     5581 caagctatat ggcacgttta gtcggtcaga aaaaagcgcg tgaaatttgg ttcttatgcc
     5641 gtcaatataa tgcacaagaa gcgttagata tgggcttaat caataccgtt gtgccttatg
     5701 ctgatttaga aaaagagact gtgcgttggt gtcgcgaaat gctacaaaac agcccgattg
     5761 ccttacgttg cttaaaagca gcattgaatg cagattgtga cggtcaggct ggcctgcaag
     5821 agcttgctgg taatgcaaca atgttgttct atatgacaga agaagggcaa gaaggacgca
     5881 atgcgttcaa cgagaaacgt gagccggact tcagcaaatt ccgccgcaat ccataattga
     5941 cggcaaatac aagcggttcg attttgcaaa aaatttgcaa aaatcgaccg cttacctttt
     6001 ctaatttaag ttaattttat gtcagatgtt gagttaaacg agcagagcga gcaagagcct
     6061 gagcaaaaac cgaaaaagcg aaactatttc ctttcaacct tattcaaaat cggggtgata
     6121 ggagcttgca gtatggcttt ttacggcatt tatttagacg gtaaaatccg tagcaaaatg
     6181 gatgggcaga tatgggaatt accagctgag gtgtatgctc gtattgaaag cattaaaatt
     6241 gaagacaact taacgcttga gcaaaccaaa caagcgttgc tggataacgg ctatcgtcaa
     6301 gtttctcaag ttgcgacacc aggcgatttc aaaattgaag aaaatcagct ggtgttgcta
     6361 cgccgagctt tcccgtttcc agaaacaccg gaatcgcaac gagtgcttcg tttaactttc
     6421 aaacaagata aattaactta tattgaagat ttggtgcagg gtaagttgat cgaggagttc
     6481 cgcttggatc cgaaattact ggcgttactg cattccgata gcgatgaaga acgtcaagca
     6541 ttacgcttac aacaataccc tcgcttgtta attgagtcac tgattttaac cgaagatcgc
     6601 cgcttttatc agcacgatgg gattagccca atcggtattg cccgtgcttt aatcactaac
     6661 taccaagctg gcagaacagt gcagggtggc agtaccttaa cgcaacagtt agtgaaaaac
     6721 ttgttcttaa gtaatgaaag atcgttggtg cgtaaggtaa atgaagcgtt aatgtcggtg
     6781 attttagact tccgttacga taaaaaccgc attttagaaa cctatctcaa cgaaatttat
     6841 ttagggcaga acggtagcta ccaaattcac ggttttgcat tggcaagcca attctatttt
     6901 ggtcgtccga tccaagagat tagcttagac caaattgcgt tattggtggg aatggtaaaa
     6961 ggtccatcac tgtataaccc gtggcgaaat ccgcaaggtg cggtagagcg acgtaatgtg
     7021 gtgttgcgat tattgctgga tcatcaagtg atcaatcaag aactttacgc tctgttaagc
     7081 tcccgcccgc ttaatattcg tgaaaaaggc acgatttacc gtgagcaacc tgcctttatg
     7141 caggcattaa ccattgattt gaaaaatgag ttaggcgaaa ttaagtcagc tcaactttcc
     7201 ggtactaaaa tttttacgac ccttgaccgt aaacagcaac gttcagccga acaagcagtg
     7261 attaccggtt tggaagatct ggaaagtaaa aataaaaaag tcagcgattt gcaatcggct
     7321 attgtcgtgg cggaataccg cacaggtaaa gtgagggcga tagtaggaga ccgcttaacc
     7381 caatttgccg gctttaaccg tgcgattcag tcaaaacgcc aaatcggttc tttagtcaaa
     7441 ccatcggttt atgcgattgc cttggcacag cctgaattat tccgtttaaa tacaccgatc
     7501 caaaataaac aaatttcaat taaggttggt tcaacctact ggacaccccg taactacgac
     7561 cgcagtttta gtggctcggt gatgttaatg gacgccttag tccgctctct gaatattcca
     7621 acggtgaata ttggtatgaa agttggctta aaaaaagtca ttgccaaaca aaaagagatg
     7681 ggctgggata atgcggaaat tccgccttac ccatcaacct tgctaggttc atattcgatc
     7741 tcgccttacg atgtaacccg ctcttatcaa gtaattgcca acgaagggct gaaaacacca
     7801 ttaaccacca ttgaggcgat tatttcgcac aatggcgaaa tgttctatca gcgagatagc
     7861 ggcaaagtga gcgagcaggt gcttccaccg gaagctgcgg ttcaaacaat gtatgcgatg
     7921 caacaggttg tggaacgagg tacggctcgt agcttacaaa acgattttgc caaactgaaa
     7981 ttagccggca aaaccggtac aaccaataac gctcgagata cttggtttgt gggaattgat
     8041 ggcgaacacg tcacaaccgt ctggctgggt aaagataaca atggggatac caacctcacc
     8101 ggttcaagtg gtgcattgta tgtgtataag caatatcttg agagagcctt gcctcaggcg
     8161 tatagattac cgaaacttaa tacgatgcaa tgggtgggga ttaatagcta tggtagctgg
     8221 aattgcgata gtggcagaca aattccagtt tggcgagata aagggcaacg ttattgtacc
     8281 agtgcaggta atgtagtggc tacagaaaaa ccaagtgttt gggatgcact ttcgctgaaa
     8341 aaagaaaata tcgaggctaa agcagaagaa gctgaaggga agcaaccaat ccctactgaa
     8401 gaagttgcac caattgaata aaaataaatt tgatagccat tatcattcaa gatacacaag
     8461 c
//
LOCUS       DANLZC010000110         8376 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.110, whole genome shotgun sequence.
ACCESSION   DANLZC010000110 DANLZC010000000
VERSION     DANLZC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.110"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..823
                     /locus_tag="PXE44_001928"
     CDS             <1..823
                     /locus_tag="PXE44_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDL5589411.1"
                     /translation="SQKIYQDKQSYLVEIEKSMNDYAELVKRYEKLLKVGHSSHDEVN
                     IQKSRYFQQKSLFNELKQELIQLKSTQLSLENEIETRKTEFNNQIIRYEMQKSDLNIR
                     LMEFESVSDIIVNASLDGKIESTSVTVGQIIKENDPLAQILPQNKGNYQLVMWVPNSA
                     ISFIKQGDEVNIRYEAFPFEKFGQFKGKITSISTLPASLQELSFYKNLPVNLEQGIPL
                     YKILIELADQNVKYNDTSLYFMSGMKAEATLFLEKRKLYEWIFFPMYQLRKNMEQ"
     gene            823..1614
                     /locus_tag="PXE44_001929"
     CDS             823..1614
                     /locus_tag="PXE44_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HDL5589412.1"
                     /translation="MTGIKQLKFDFRRRIPVILQTETAECGLACLAMMLGYYDKNTNL
                     FELRSQYGTSSRGVTLHTLISIANDCGLITRPLSLELDEVPQLRLPCILHWDFNHFVV
                     LTHASEKQFIIHDPAFGKRKLSRAEFSNHFTGVALEVWSEVKFEKSNNENTISLYETL
                     KHISGIKGALVKIFALSMLIELIALLMPIGTQLVMDHVMQAKDQSLLLIICIGLFLFT
                     LFRTMVSMFRAWVSLKMNYLIDFQWTASFFSHLLKLPLDFFENAK"
     gene            1992..2909
                     /locus_tag="PXE44_001930"
                     /pseudo
     CDS             1992..2909
                     /locus_tag="PXE44_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2911..3579)
                     /locus_tag="PXE44_001931"
     CDS             complement(2911..3579)
                     /locus_tag="PXE44_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDL5589413.1"
                     /translation="MKYLYALIFSLFFSSVYAAERQLQCKVVGISDGDTLTCLVNKTP
                     LKIRLQHIDAPEQAQPYGNKAKQALAQFVFQQNITAIVSGYDRYQRLLATLYLNDKNI
                     NLALVENGMAWAYTTKREYIEAQDQAQQAKIGLWQDKFPINPSQWRKTHTSTLPQAVK
                     KPENIANQPPTIDCQIKLSCNQIGNYAQAKRYFQQCGWKELDGNNDSIPCNKLYRQEQ
                     KNRN"
     gene            complement(3623..4990)
                     /locus_tag="PXE44_001932"
     CDS             complement(3623..4990)
                     /locus_tag="PXE44_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDL5589414.1"
                     /translation="MSIDTILNQINSFVWGPPLLILISGVGLYLTFSLKGIQLSQLGQ
                     AFRYMFKPEKGKGQSGDISAFAALSTALAATIGTGNIVGVATAIHSGGPGALFWMWLI
                     ALFGMATKYAEGLLAIKYRTKDRAGFVAGGPMYYIELGMGKKWRWLAKAFAFFGVLVA
                     LLGIGTFPQVNGITHALESTFDVPIIFSAIILTLIVTAIILGGVQRISKIATIVVPFM
                     AIGYVIAALSVILLNMEKVPQAIITIVHAAFNPQAALGGAVGFTVMQAIQLGVARGIF
                     SNESGLGSAPIAAAAAQTKEPVRQGLISMTGTFLDTIIVCTMTGLVIVISGAWAGDVK
                     GAELTTTAFTQGLNSSFGAIIVTVGLVFFAFTTILGWCYYGERCFVYLTRGRTQYIKL
                     YRLLFIALIASAPFLQLDTIWTLADIVNGLMAFPNLIALIALRKVIISETKDFFARLK
                     TGKAE"
     regulatory      complement(5109..5204)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(5375..6427)
                     /locus_tag="PXE44_001933"
     CDS             complement(5375..6427)
                     /locus_tag="PXE44_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL5589415.1"
                     /translation="MTHSTKTLVAIASIIIILAGIKLSNEIVVPFLLSLFIAIICSPM
                     IKFMTKRKVPLWLAITILLIVFILGFSFLAGIINNTLQEFTNAIPQYKIQLSSRIQVI
                     LELAHRWHIPLTLSQEEIMSKFDPSSIMNLVSKLLMGLSGALSNIFVIFLVVIFMLLE
                     MPVAKYKLALALSPDDDLSNEKEYINQVLEGVIGYLGVKTFVSLLTGGLVWILLEILG
                     VEYAILWAILTFLLNYVPNIGSILAGIPIVIQALVLNGFSIGLGTLIGIVVINMVVGN
                     ALEPKLMGKKLGLSTLVVFLSLLFWGWLLGTVGMLLSVPLTMACKVALESNKATQKYA
                     VLFSDLEEVPKEQKYR"
     gene            complement(6474..6899)
                     /gene="ruvX"
                     /locus_tag="PXE44_001934"
     CDS             complement(6474..6899)
                     /gene="ruvX"
                     /locus_tag="PXE44_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL5589416.1"
                     /translation="MTETTTKTVIAFDFGTNSIGCAVGQSITGTAQGLTTFKAQDGIP
                     NWEEIGKLLQDWKPDMLVVGLPLNMDGTEQPLTQRARKFANRLNGRFNLPVELQDERL
                     TTVEAKAEIFSRGGYKALKKDKVDSISACLILESWFENH"
     gene            complement(6899..7459)
                     /locus_tag="PXE44_001935"
     CDS             complement(6899..7459)
                     /locus_tag="PXE44_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDL5589417.1"
                     /translation="MLENLQGKFLIATPEMDDDYFERTVVYICEHNPQGAMGVVINTP
                     TDLSVLELITRMDFQMANQRDYKKDQMVLSGGPVNQDRGFIIHTKTANDFIHTYPITD
                     KITLTTSGDVLDSFGTLASPEKFIVCLGCSTWRGSQLEQEIAQNYWIVSDANEKTLFE
                     TGYLERWAEAYEMMGLSSIPSRAGRA"
     gene            complement(7459..8190)
                     /gene="rsmE"
                     /locus_tag="PXE44_001936"
     CDS             complement(7459..8190)
                     /gene="rsmE"
                     /locus_tag="PXE44_001936"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL5589418.1"
                     /translation="MRIPRIYQPQPLAGLQSCVLSEDAANHVGRVLRMKQGEQIILFD
                     GSNHVFHATLQAVEKKQIIAKIDSSELDDRESNLPIHLGQVISRGDRMEFTIQKSVEL
                     GVKTITPLWSERCGVKLNDERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWC
                     KEQDDMLKLNLHPRAKYTIRELPNVPKAGVRLLIGSEGGLSAEEIAMTESEGFTEVLL
                     GKRVLRTETASLAAITALQVCFGDI"
BASE COUNT         2517 a         1608 c         1661 g         2590 t
ORIGIN      
        1 cagtcaaaaa atttatcaag ataagcagag ttatttagtg gaaattgaaa aaagtatgaa
       61 tgactatgcg gagttggtta aacgctatga gaaattatta aaagtagggc attccagcca
      121 cgatgaagtg aatattcaga aaagccgtta tttccaacaa aaatcactgt ttaatgagct
      181 aaagcaagaa ttaattcaat taaaatccac ccaacttagc ttggaaaatg aaattgaaac
      241 ccgtaaaacc gaatttaata atcagattat tcgctatgaa atgcaaaaaa gcgatttaaa
      301 cattcgttta atggaatttg aatctgtttc agatattatt gttaatgcct ctttagatgg
      361 aaaaattgaa agtaccagcg taacagttgg gcaaattatt aaagaaaatg acccgcttgc
      421 acaaattctg ccacagaata aaggaaatta tcagctagtg atgtgggtac cgaatagtgc
      481 catttcattt attaaacaag gcgatgaggt gaatattcgc tatgaggctt ttccgtttga
      541 aaaattcggg cagtttaagg gcaaaattac gtcaatttca accttaccag cttctttgca
      601 agaacttagt ttttataaaa atctccctgt aaatttagag caaggtattc cgctttataa
      661 aattctgatt gagcttgccg atcaaaacgt aaaatataat gatacctccc tttattttat
      721 gagtggaatg aaagccgaag ccacgttatt tttagaaaaa cgtaaactgt atgagtggat
      781 atttttccct atgtatcaat tacgcaaaaa tatggagcaa taatgacagg aattaagcag
      841 ttaaaatttg attttcggag aagaatacct gttattttgc aaactgaaac agctgaatgt
      901 ggcttagcct gtttagcgat gatgttgggc tattacgata aaaacaccaa tttatttgaa
      961 ttgcgaagtc aatatggtac ttcctctcgt ggcgtaacgc tgcatacttt aatttctatt
     1021 gcaaatgact gtggtttaat aactcgccct ctttctctag agttagatga agtaccacaa
     1081 cttcgtctgc cttgtattct tcattgggat tttaatcatt ttgtagtatt aactcacgca
     1141 agtgaaaaac agtttattat ccacgatcct gctttcggta aacgtaaatt atcccgtgct
     1201 gaattttcca accattttac cggagtcgct ttagaagttt ggtctgaagt taaatttgag
     1261 aaaagcaaca atgaaaatac cattagtttg tatgaaacct taaagcatat ttctggcata
     1321 aaaggtgcat tagtgaaaat ttttgccctt tcaatgctca ttgaactcat tgccttattg
     1381 atgccaattg gaacacagtt agtgatggat cacgttatgc aagcgaaaga tcaatcatta
     1441 cttttaatta tctgtattgg gctattttta tttacccttt ttcgcacaat ggtcagtatg
     1501 tttagggctt gggtttcctt gaaaatgaat tatttaattg attttcaatg gacagccagt
     1561 ttcttttctc atctcttaaa attaccgctt gatttctttg aaaacgccaa gtaggtgata
     1621 ttcattcccg atttagttca ttaaaaacca ttcagaaaac tctaacttca agcattatta
     1681 ccacgattat tgatacgatt atgataatca gtttaattat tatgatggtg ttatatggcg
     1741 gttggctctt ttgggtagta ttagcttttt cctttatcta ttttattctt cgaatggtta
     1801 cttatttagt ttatcgtcaa ctgaatgaag agcagattat taaaggagca aaagcccatt
     1861 cgcattttat ggaaacttta tatggtatta caacgttaaa atcattagga ttggaaaata
     1921 aacgacaaga acagtggatg tcgctcaatg cggatgcttt taacacatca attaaggtaa
     1981 ctaaatttga tatgctgttt tccggtattc atacctttat ttctaccatc gaacagattt
     2041 taattctttg gattggggcg acaatgatga ttgaaaatgt gatgacatta ggaatgtttg
     2101 tcgcttttaa tgcctatcga ggctctttct cttcacgaat ggggaattta attgatatta
     2161 tttttagcct aaaaattctt tcattacata gagagcggat tgcagacatt gctttaaatg
     2221 aagtcgaaga tgaaagtaaa tatcaagaac tcagatttga acataaccaa acagatatag
     2281 aagttaaaga cctttgtttc aaatacgatc ctttttctaa aaatgtgctt gaaaatgtca
     2341 atttagtgat cagaagtggg gaaagtgttg ctattaccgc accatcaggt tatggcaaaa
     2401 caacgttatt aaaattaatt gcaggtttat taaaaccaac ctcaggttct gtgtatttta
     2461 acggcattga tatttatcag ttaggattaa gtcaatatcg gagccaaatt gcttgcgtat
     2521 tacaggaaga taaattcttt tctggttcca ttttagagaa tattgttagt tttgaaagta
     2581 attatgatcg agaatgggct atcgaatgta gcaaacttgc gaatattcac aatgatatta
     2641 tggcaatgcc gatgaattat gaaaccttat taggagaatt aggaaataat ttgtctggcg
     2701 gacaaagata acgattgttt attgctcgag ctttatataa aaagcctaaa attcttttga
     2761 tggatgaggc gactagccat ttagatgaag aaaatgagaa gcttgtcaat caagctattt
     2821 cacgaatgaa gataacaagg attattattg ctcatagaca atcgactatt cagtcggcgg
     2881 atagaattat tgccctgaat gacaggtaaa ttaattccta tttttctgtt cttgccgata
     2941 aagtttattg cacggaatgc tgtcgttgtt gccgtctagc tctttccagc cgcattgttg
     3001 aaagtagcgt tttgcctgtg cgtaattgcc gatttggttg cagcttaatt taatttggca
     3061 atcaatcgtt ggtggctggt ttgcaatatt ttccggtttt ttgaccgctt gtggtaaggt
     3121 tgaggtgtgt gtttttcgcc attggctcgg gttaatcggg aatttgtcct gccataagcc
     3181 gattttagct tgctgtgctt gatcttgtgc ttcgatatat tcccgctttg tggtgtaagc
     3241 ccacgccata ccgttttcca ctaaggctaa gttgatgttt ttatcgttta agtagagggt
     3301 ggcaagtaat cgttggtaac gatcatagcc tgaaactatg gcagtaatgt tttgttgaaa
     3361 cacgaattga gccagagctt gtttggcttt gttgccgtaa ggctgcgctt gttcgggggc
     3421 atcaatatgt tgtagcctta tttttagtgg tgttttattg accaaacaag tgagggtgtc
     3481 gccatcgcta atgccaacga ctttgcattg tagttggcgt tcggcggcat ataccgatga
     3541 gaaaaataag ctaaaaatca gggcgtaaag atatttcatt attaaaaaag agagggctaa
     3601 taagccctca ttctatcatt ttttattcgg ctttacctgt tttaaggcga gcgaagaaat
     3661 cttttgtttc actaataatc actttgcgta acgcaatcaa tgcgattaaa ttcgggaatg
     3721 ccattaagcc gttcacgatg tctgccagcg tccaaattgt atcgagttgt aaaaatggag
     3781 cagaggcgat gagtgcaata aacagcaagc ggtataattt aatgtattgg gtgcgacctc
     3841 gagtgagata gacaaaacag cgttcaccgt agtagcacca gcctaaaatg gtggtaaagg
     3901 caaagaagac taaaccaact gtcacaatta tcgcaccgaa tgagctgttt aaaccttgag
     3961 taaaagcggt tgtggtgagt tctgcgcctt ttacatcgcc tgcccaagcc cctgaaatca
     4021 caattactaa gccggtcatt gtgcagacga taatagtgtc taagaacgtg ccggtcattg
     4081 aaattaggcc ttgacgtacc ggctctttgg tttgggcggc agcggcggcg atgggggcag
     4141 agcctaatcc tgattcattc gagaaaatac cacgtgcaac ccctaattga atcgcttgca
     4201 tgacggtgaa tccgactgct ccgcctaatg ccgcttgtgg gttgaaagct gcgtgtacaa
     4261 tagtaataat tgcttgtggg actttttcca tattcagtag aatgacggat aaagcggcga
     4321 tgacataccc aatagccata aacggtacaa caatggttgc aattttagaa atccgctgca
     4381 cgccacctaa aataatagcg gtaacgataa gggttaaaat gatcgcactg aagataatcg
     4441 gcacatcaaa ggtgctttct aaggcgtggg taatgccatt cacttggggg aacgtgccaa
     4501 taccaagcaa agcgaccaat acgccaaaaa acgcaaaggc tttagcaagc caacgccatt
     4561 tttttcccat tcccagttcg atatagtaca tcggaccacc agccacaaat ccggctctat
     4621 ctttagttcg atatttaatt gccaataagc cttcggcata tttcgtagcc ataccgaata
     4681 aggcaatcag ccacatccaa aataatgctc caggcccgcc cgaatgaata gcggttgcca
     4741 cgccgacaat attccccgtg ccgatagtag ctgctagggc agtggaaagg gctgcaaaag
     4801 cggaaatatc gcccgattgc cctttgcctt tttccggttt gaacatatag cgaaaggctt
     4861 gccctagttg gcttaattga atacctttga gtgaaaaggt taaatataac cctacgcctg
     4921 aaattaaaat caggagtggc gggccccaaa caaaactgtt gatttgatta agaatagtgt
     4981 cgatagacat ttgatgttcc tcgtaaaaac tgcttacaag gaaagaaaag gaatgcaaga
     5041 gacaagcggt caaaaaacgg aaattttttg caaagaatta gcaaaaaaac accgcttgta
     5101 tcgtataact tttctcccct gtccttttgc ctgagcgttt cacacattgc tgtattttcg
     5161 ccttcggcgg cggattttaa tccgctctct ccaagggttc gtccagtaac agtccccgca
     5221 gtgttcactg cacctgaaag agtttacctc gtcggtgaaa tgttaattca tttctctcca
     5281 gctaccttca tccgaacaat ctaatttatt ttataatgta aaataaaaaa gatttgccaa
     5341 attctagcaa ctcttttgct tccgtcaagt gaatttaacg atatttttgt tctttcggta
     5401 cttcttctaa atcgctaaat aatacggcat atttttgggt ggctttattg gattctaatg
     5461 ccactttaca cgccatcgta agtggaacag aaagtaacat tcctacggtg ccaagtaacc
     5521 acccccaaaa tagaagtgag aggaacacta caagggtaga aagcccgagt ttcttgccca
     5581 ttaatttagg ttcaagcgca ttacccacaa ccatattaat cacgacaatg ccgattaaag
     5641 tgcctaatcc tatagaaaag ccattgagta ccaaggcttg aatcacaatt ggaattcccg
     5701 ccaaaataga gccgatattc gggacgtagt taagcaggaa ggttagaatt gcccataaaa
     5761 tcgcatattc tacgcctaga atttctagta agatccaaac taaccctccg gtgagtaggc
     5821 tcacaaaggt tttaactcca aggtaaccaa tcaccccttc aagtacttgg ttgatatact
     5881 ctttttcgtt gcttaaatca tcatctgggc taagtgcgag ggctaattta tatttggcaa
     5941 ccggcatttc caatagcata aaaatgacga ctaagaaaat cacaaaaata ttcgataatg
     6001 caccggatag ccccattagc agtttgctga ccaaattcat tattgagctt gggtcaaatt
     6061 tgctcataat ctcttcttgt gagagggtaa gtggaatatg ccatctatgg gcgagttcta
     6121 gaatcacctg gattctagat gaaagctgaa ttttgtattg cggaatagca ttagtaaatt
     6181 cttgcaaggt gttattaata atgcctgcta agaaactgaa tcctaagata aaaacaataa
     6241 gtaaaatggt aatggctaac cataatggca ctttacgctt agtcataaat ttgatcattg
     6301 gtgaacaaat aatggcaata aatagcgaaa gtaaaaatgg cacaacaatt tcattggata
     6361 atttaatccc tgccagaata atgataatgg aggctatcgc cacgagagtt ttggtggaat
     6421 gtgtcataat ttttcctttt acaagcggtt aaaaatcagc aattttttac aaattagtga
     6481 ttctcaaacc aactttctaa aattaaacaa gcggaaatgg aatccacttt atcctttttt
     6541 aatgctttgt aaccaccacg agaaaaaatt tccgcttttg cttcaacagt agtgaggcgt
     6601 tcatcttgta gctcaaccgg cagattaaag cgtccgttta agcgattggc aaattttctg
     6661 gcacgctgag ttaaaggctg ctctgtgccg tccatattca agggtagccc aacaaccagc
     6721 atatctggtt tccaatcttg caagagtttt ccgatctctt cccagtttgg aataccgtct
     6781 tgggctttaa atgtagttaa accttgagct gtgccggtaa tactttgtcc aactgcacag
     6841 ccgatgctat ttgtgccaaa atcaaaggca atcacggttt ttgttgtggt ttctgtcatt
     6901 aagctctccc agctcttgac ggtatgcttg aaagccccat catttcatag gcttctgccc
     6961 aacgctctaa ataaccggtt tcaaacagcg ttttttcatt cgcatcagac acaatccaat
     7021 aattttgagc aatttcttgc tcaagctggc tgccacgcca agtggaacaa cctaaacaca
     7081 caataaattt ttcaggtgaa gccagtgtgc caaaggagtc taacacatca ccggaggtag
     7141 tgagcgtaat tttatcggtg atcggataag tgtgaataaa atcattggcg gttttggtgt
     7201 gaataataaa gccacgatct tgatttaccg gcccaccgct taacaccatt tggtcttttt
     7261 tgtaatcacg ctggttcgcc atttggaaat ccattcgggt aatcagctct agaaccgaca
     7321 gatcggtagg agtgttaatt actactccca ttgcaccttg cggattatgt tcgcaaatat
     7381 agaccaccgt acgctcgaaa tagtcatcgt ccatttccgg cgtggcaatt aaaaatttac
     7441 cttgtaaatt ctctaacatt aaatatctcc aaaacaaact tgtaacgcag taattgccgc
     7501 aagtgaggcg gtttctgtgc gtaacactcg tttacccagt aatacttcag taaaaccttc
     7561 gctctcggtc atggcgattt cttcggcaga taatccgccc tcagagccaa tcagtaaacg
     7621 aacaccagct ttcggcacat tcggcagttc acgaatggta tatttggctc ttgggtgcaa
     7681 attcagtttc aacatatcgt cttgttcttt gcaccagtcg gtgagtttca taatcgggcg
     7741 aatttcaggg atttcatttc gtccgcattg ttcacaggcg gcaatcgcaa ttttctgcca
     7801 ttgctgtaat tttttatctt gccgttcatc attgagtttc acgccacaac gttccgacca
     7861 aagtggggta atagttttta cccccagctc caccgatttt tgaatggtaa attccattct
     7921 atcgcctcgg gaaatcactt gccctaaatg gatcggcagg ttggattctc ggtcatctaa
     7981 ttcacttgaa tcaatttttg caataatttg ctttttttcg accgcttgta acgtagcgtg
     8041 gaaaacgtgg tttgaaccat caaataaaat gatttgctct ccttgtttca ttcgtagcac
     8101 tcgcccaacg tggttagcgg catcttcact taacacgcaa ctttgaagac cagctaacgg
     8161 ctgtggttgg taaattcttg ggattctcat aattatctag ttataaatta aaggcagacg
     8221 attgtctgcc ataggtaagt taaaacgggt taaggataaa gtcttgaaca atgcacaata
     8281 gtaataatcg aaatttcatt ctctttaact tcacacacta ttctatagcc gttatgatat
     8341 agttctctcg tattaataat tgcacctttt acaccc
//
LOCUS       DANLZC010000111         8277 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.111, whole genome shotgun sequence.
ACCESSION   DANLZC010000111 DANLZC010000000
VERSION     DANLZC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.111"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(374..2044)
                     /gene="glnS"
                     /locus_tag="PXE44_001937"
     CDS             complement(374..2044)
                     /gene="glnS"
                     /locus_tag="PXE44_001937"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDL5589419.1"
                     /translation="MSEEILTAETGTRANFITHIIDEDLASGKHNNVYTRFPPEPNGY
                     LHIGHAKSICLNFGIAQDYNGLCNLRFDDTNPVKEDVEYVDSIKQDVEWLGFKWEGEP
                     RYASDYFDQLYGYAIELINKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRSVEENL
                     ALFEKMKNGEFAEGKACLRAKIDMASPFIVMRDPVLYRVKFAHHHQTGDKWCIYPMYD
                     FTHCISDAIERITHSLCTLEFQDNRRLYDWVLDNISIERPLPHQYEFSRLNLEGTLTS
                     KRKLLKLVTDGIVDGWNDPRMPTISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSAL
                     EACIREDLNENAPRAMAVINPVRVVIENFGEKEILKAPNHPNRPELGERDLPFTREIY
                     IDEADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGRISTIFCTYDPDTLGK
                     NPADGRKVKGVIHWVSAEDNKPAEFRIYDRLFTVPNPGAEEDINAVLNPESLVVKHGF
                     VESSLAHAKPEQDYQFEREGYYCLDSKDGSADNLVFNLTVSLKESVAF"
     gene            complement(2205..2648)
                     /locus_tag="PXE44_001938"
     CDS             complement(2205..2648)
                     /locus_tag="PXE44_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL5589420.1"
                     /translation="MNLQQIQDRLALKDLVDTFSNLADEKKVAEQMPLFTQDAVVNTY
                     IGGKLVFEMHGVAEIEQVFTTFLTPFHSVYHLNGQHTVPFIDENNATAINYCAVKLVE
                     TKEGKEILHDHSVRYADTYVKQEGKWRIAKRIANFMISESREIRQ"
     gene            complement(2699..3274)
                     /locus_tag="PXE44_001939"
     CDS             complement(2699..3274)
                     /locus_tag="PXE44_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL5589421.1"
                     /translation="MKQILILGASGSLANVVIPELLKDNNVQLTLFARRPQSVAKFET
                     ERVKIVQGDVLDFHALTQAMQGVDAVYAGLAGDLKAMAGNVVNAMNEAQVKRLVWISS
                     MGIYGETGEDHGAILEPYRQSAAVIEASELDYTVVRPGWFTNGSEVQYQLTHKGEPFK
                     GHSVSRKSIADFVARILRENSEIRESVGIAA"
     gene            3381..4271
                     /locus_tag="PXE44_001940"
     CDS             3381..4271
                     /locus_tag="PXE44_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5589422.1"
                     /translation="MKENLNDLRAFLLVAQTSSFTKAAAQMGVSQSALSHSIRGIEER
                     LQIKLFHRTTRSISTTEAGERLYQRLLPLFDEIDQEVNGLSEFRNALKGGLRINGNEH
                     VFRDVLADKFAEFVKRYPEVALELVAEDRFVDIVAERFDAGIRLGADVAKDMIALRIS
                     PDVKMTAAASPNYLAQHGTPKTPFDLTEHHCLKHRLATLGGILAWEFRDPITKKIVKF
                     QPQGNLTANNGFLLQHYAKQGLGILWSPQDSIQTELDNGSLVPILTDWAMQYEGYHLY
                     YPNRRQNSPLLKALIDVLRG"
     gene            complement(4338..4904)
                     /gene="efp"
                     /locus_tag="PXE44_001941"
     CDS             complement(4338..4904)
                     /gene="efp"
                     /locus_tag="PXE44_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL5589423.1"
                     /translation="MASYSTNDFKKGLKFIQDGEPCVIVENEFVKPGKGQAFTRTKIR
                     KLISGKVLEINFKSGTSVEAADVVDSNYNYSYSDGDFWYFMHPETFEQISVDEKALGD
                     NVKWLVDNAECIVTLWNGSAIAVTPPNFVELEIVETDPGLKGDTAGTGGKPATLSTGA
                     VVNVPLFVQIGEVIRVDTRSGEYVSRVK"
     gene            5071..5640
                     /locus_tag="PXE44_001942"
     CDS             5071..5640
                     /locus_tag="PXE44_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589424.1"
                     /translation="MKKLITLSAILAVSVATVAEAKRGGGSFRSVSPSKAVATKPVAS
                     QKSTTNQQTQQKDATFANTPNSATAQPQVRQGSTMANVAMGAAAGYILGDMMSSNAHA
                     NQPSQPADANAQAVNSAQNVANGAVPAIAEFKSIGGQIDPFLVEKTDGYRRYCISGVQ
                     YLVAAQGVQTSPTVMVNPNGTPLQCNLIP"
     gene            5694..6686
                     /gene="epmB"
                     /locus_tag="PXE44_001943"
     CDS             5694..6686
                     /gene="epmB"
                     /locus_tag="PXE44_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDL5589425.1"
                     /translation="MQPIQIKPLWLTELAQAFNNPIDLLQFLELNPNDFEGDIAARKL
                     FALRVPRMFAEKMEKGNPNDPLFLQAMSLQAEFIEAEGFVVDPLEEQQSPAPNILHKY
                     HNRLLFMLKNSCAINCRYCFRRHFPYEEVKSGKAVWQQGLTYIAEHPELEEVILSGGD
                     PLMAKDQDLDWILTQLEQISHIKTLRIHSRLPVVIPNRITTELCERLSKSRLNVVLVT
                     HINHANEIDAVFANKMAQLKKAGVVLLNQSVMLKGVNDNAQTLKRLSDKLFEYGILPY
                     YLHLFDKVAGASHFYIEDEQAGEIYRELQRITSGYLVPKLAREIAKEPNKILKG"
     gene            complement(6786..8189)
                     /gene="asnS"
                     /locus_tag="PXE44_001944"
     CDS             complement(6786..8189)
                     /gene="asnS"
                     /locus_tag="PXE44_001944"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL5589426.1"
                     /translation="MSKFPTVSEILSGKIAVGEEATVRGWVRTRRDSKAGLSFLSVYD
                     GSCFDPIQAIINNDLPNYNAEVLHLTAGCSVVVTGKVVESPAEGQAVELQASKVEVLG
                     WVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHCLAQAIHRFFHEQGFYW
                     VATPVITASDTEGAGEMFRVSTLDLENLPRGENGKVDFNQDFFGKESFLTVSGQLNAE
                     TYACALSKVYTFGPTFRAENSNTTRHLAEFWMMEPEVAFADLNDNAKLAEDMLKYVFK
                     AVLAERKDDMEFFVKHVDKEAISRLENFINSPFAQVDYTDAIEILLKSGKEFEFPVSW
                     GIDLSSEHERFLAEEHFKSPVVVKNYPKDIKAFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEVLDQRMNEMGLNPEDYWWYRDLRKYGTVPHSGFGLGFERLIVYVTGLQ
                     NIRDVIPFPRAPRNANF"
BASE COUNT         2371 a         1782 c         1719 g         2405 t
ORIGIN      
        1 tcttatctat tcttcgagaa gacaatgcta tcataaaaga gcatcacttt tggtttccaa
       61 agattataga aagctcaaat aatacactac ctcataattt aaaatgaaac gtttcaattg
      121 gaaaaagtgg attttgtgac tgagatcaca aatttaaaca ttggtctgta tttttgagag
      181 attttgtttg aaactgccgg caaattcgct taaccttaag gtaacaagaa taagtgttgt
      241 acctagcaac ctaacacata aatttgcttt ccgagtggcc ccaagaattg gggctttttt
      301 tatgcttgat gagtcgtttt gagagtaaaa aaataccagc atatttgctg gtatttgaat
      361 gaagagagtt agattagaac gctacgcttt cttttaagct gacagttaag ttaaatacaa
      421 ggttgtctgc cgaaccatct ttgctgtcta agcagtagta accttcacgc tcgaattggt
      481 agtcttgttc cggtttggcg tgagctaaac ttgactcaac aaaaccatgc ttaaccacta
      541 atgattcagg atttagcacc gcattgatgt cttcttccgc ccccgggttt ggtactgtaa
      601 ataggcggtc gtaaatgcgg aactctgccg gtttgttatc ttcagcagat acccaatgaa
      661 tcacgccttt tactttgcga ccatcagccg gatttttgcc caatgtatcc gggtcgtatg
      721 tgcaaaaaat agttgaaatt cgaccgcttg catctttttc cacacgctca gctttaatca
      781 cataggcatt gcgtaaacga acttctttgc ctaagactaa acgtttatat tgcttattgg
      841 cttcttcacg gaagtcggct tcatcaatat aaatctcacg ggtgaacggt aaatcacgct
      901 cgccaagctc tggacgattt gggtgattag gggcttttaa tatttccttt tcaccgaagt
      961 tttcaatcac cactcgaacc gggttaatca ccgccatcgc gcgtggtgcg ttttcattta
     1021 agtcttcacg aatgcacgct tcaagggcag agtattccac cacgttatct tgcttggtta
     1081 ccccaatacg gcgacaaaat tcacgtaaag acgctggggt ataaccacgg cggcgtaagc
     1141 cggaaatggt tggcatacga gggtcattcc aaccatcaac aatgccgtct gtgactaatt
     1201 ttaataattt acgcttagag gttaaagtac cttctaaatt taaacgtgaa aattcgtatt
     1261 ggtgcggtaa cggtctttca atcgaaatat tgtccagcac ccaatcatat aagcggcggt
     1321 tatcttggaa ctctagcgta catagcgagt gagtgattcg ctcaatcgca tcggaaatgc
     1381 agtgggtaaa atcgtacatc gggtagatgc accatttatc accggtttga tgatggtgag
     1441 caaatttcac acggtaaagc accggatctc gcatcacaat aaacggggac gccatatcaa
     1501 tttttgctct caagcaggct ttcccttccg caaactctcc gtttttcatt ttttcgaata
     1561 acgctaagtt ttcttccacg ctacgatcac ggtaagggct gtttttgccc ggctcggtta
     1621 aagtaccacg gtattcacgc atttcatcag gcgataattc atcaacatag gctaatcctt
     1681 tgttgattaa ctcaatcgca tagccataaa gctggtcaaa atagtctgag gcataacgag
     1741 gctcaccctc ccatttaaaa cctagccact ccacatcttg cttaattgaa tctacatatt
     1801 ccacgtcttc tttaaccggg ttggtatcat caaaacgcaa gttacataaa ccgttataat
     1861 cttgagcgat accaaaattt aaacagatag atttagcgtg tccgatatgc aaataaccgt
     1921 ttggttcagg tggaaaacga gtgtaaacat tgttgtgctt tccgcttgct aaatcttcat
     1981 caataatatg ggtaataaaa ttggctcgag tgccagtttc tgccgttaaa atttcttcgc
     2041 tcatagttct ctctaaatgt atcaataaaa ataacggatt attctacact taattgaaac
     2101 agacttaaag actaaagcaa aattcctcgg tttcctccaa agaattttgc tgttttagta
     2161 agatgcaatg gaattgcaaa tttttagcta aatttaaccg cttgttattg cctgatttca
     2221 cggctttcgc tgatcataaa attcgcaatc cgtttggcaa tccgccattt accctcttgt
     2281 ttaacatagg tatcggcata acgcacgctg tgatcgtgca agatctcttt gccctctttg
     2341 gtttctacca gtttcaccgc acaatagttg atcgctgtgg cgttattttc atcaataaaa
     2401 ggcacggtat gctgaccgtt taaatgataa acgctgtgaa acggggttaa aaaggtggta
     2461 aacacctgtt cgatttctgc cacgccgtgc atttcaaaca ccaattttcc gccgatgtag
     2521 gtgtttacga cggcatcttg cgtgaaaagc ggcatctgct cggcaacctt tttttcatcg
     2581 gcaaggttag agaatgtatc tactaaatct tttagtgcta aacgatcttg gatttgttgt
     2641 aagttcattg tgttttcctt ctgatttttg caaattttct ttggtttttc accgcttgtt
     2701 aggctgcaat accaacactt tcacggattt cgctgttttc acgcaaaatg cgagccacaa
     2761 aatcggcaat ggatttgcgg gaaacgctgt gtcctttgaa cggttcgcct ttgtgagtca
     2821 gctgatattg cacttccgaa ccgttggtaa accagcccgg gcggacaacg gtataatcca
     2881 actcgctggc ttcaatcacg gctgccgatt ggcggtaagg ctctaaaatt gccccgtgat
     2941 cttcgccggt ttcgccataa atccccattg agctaatcca aaccaagcgt tttacctgag
     3001 cttcattcat tgcgttcacc acatttcccg ccatcgcttt taagtcgccg gcaagtccag
     3061 cgtaaacggc atcaacgcct tgcattgctt gggttaatgc gtggaaatcc aacacatcgc
     3121 cttgcacaat tttgacgcgt tcagtttcaa atttcgctac tgattgcgga cgacgggcaa
     3181 aaagggttaa ttgcacatta ttgtctttta gcagttcagg aatcacgaca ttcgctaggc
     3241 taccgcttgc tcctaaaatt aagatctgtt tcattgctgt tctccattga atgaagtcag
     3301 tatagcggtg tagattgcat ttgattagta gggtaaaatc gcttgaagta atgagaaaaa
     3361 ttcattaata aggtggttaa atgaaagaaa acctcaacga tttaagagct tttttgttgg
     3421 tagcacaaac gagcagtttt accaaagcag cagcacagat gggcgtgtcg cagtcggcgt
     3481 tgtcgcacag cattcggggg attgaagaac ggttgcaaat caagctgttt catcgcacca
     3541 ctcgtagcat ttcaaccacc gaggcagggg agaggctgta tcaacggctg ttaccgctgt
     3601 ttgatgaaat cgaccaagaa gtaaatgggc tgagtgagtt tcgcaatgcg ttaaaaggcg
     3661 ggctgagaat taacggtaat gaacacgttt tccgtgatgt gctggcagat aaattcgctg
     3721 agtttgtcaa acgttaccct gaagtagcat tggagctagt ggcggaagat cgctttgtag
     3781 atattgtcgc cgagcgtttt gatgccggta ttcggcttgg ggcagatgtg gcgaaagata
     3841 tgattgcact acgcatttcg cccgatgtga aaatgaccgc agctgccagc cccaactatt
     3901 tggcacagca cggcacgccg aaaacgccct ttgatttaac cgaacatcat tgtctcaaac
     3961 atcgtcttgc gacattgggc ggcattctgg cgtgggaatt tcgggatccc atcaccaaga
     4021 aaatcgttaa atttcaaccg cagggcaatc ttactgccaa taacggcttc ttgctgcaac
     4081 actatgccaa acaaggttta ggcattcttt ggagtccgca agacagcatt caaaccgagc
     4141 tggataacgg cagcctcgtg ccgattttaa ccgactgggc aatgcagtat gagggctatc
     4201 atctctatta ccccaaccgt cgccaaaatt cgcctttgct gaaagcgtta attgatgtgc
     4261 tgagaggata ggtaaaaatg agcggtcgtt tttagcaaaa aattgaccaa aaaacgacca
     4321 cttgtagaga agctaaatta tttcacacga gaaacatatt cgcctgaacg ggtatccaca
     4381 cggataactt caccgatttg aacgaataaa ggcacgttta ccacagcacc ggtgcttaat
     4441 gttgccggtt taccgcctgt acctgcagta tcgcctttta agcccgggtc ggtttcgacg
     4501 atttctaact caacgaagtt tggtggtgtg actgcaattg ctgaaccgtt ccataaagtc
     4561 acgatacatt ctgcattatc aactaaccat tttacgttgt cgcctaacgc tttttcatct
     4621 accgagattt gttcaaaggt ttcagggtgc ataaagtacc agaaatcacc atcgctgtaa
     4681 gagtagttgt agttagaatc tactacatct gctgcttcaa ccgaagtgcc tgatttgaag
     4741 ttgatctcta atactttgcc agagattaat ttacggattt tagtacgggt aaatgcttga
     4801 cctttacccg gttttacgaa ttcgttttca acaattacac aaggttcgcc atcttgaata
     4861 aattttaaac cttttttaaa gtcgttagtg ctgtagctag ccattatttc ctcaattatt
     4921 taaaaaataa caataaatta acaaattttg aacccaaccc tattgggttt gtcaaaaaga
     4981 gccatataat aacctaaatt cacaaaatta gcgaaagatc tcttgcgatt tttgctataa
     5041 tttttcaaac tttattgata aggaatgcga atgaaaaaac tgattactct ttctgcgatt
     5101 ttagccgttt ctgttgcaac cgtggcagag gcaaaacgtg gcggtgggag cttccgttca
     5161 gttagcccgt caaaagcggt agcaactaag ccggtagctt ctcaaaaatc aaccacaaat
     5221 cagcaaacgc aacaaaaaga tgctactttt gccaatacgc caaacagtgc aactgcccag
     5281 ccacaagtgc gtcaaggcag cacgatggca aatgtagcga tgggggcagc agccggttat
     5341 attttaggtg atatgatgtc atcaaatgca catgcaaatc agcctagcca gcctgcggat
     5401 gcaaatgcac aagcggtcaa ttctgcacaa aatgttgcaa atggagcagt tcctgctatt
     5461 gcagaattta agagtattgg cggtcaaatc gatccgtttt tagtagaaaa aaccgatggt
     5521 tatcgccgtt attgtatttc aggcgtgcaa tacctagtcg ctgcacaagg tgtacaaacc
     5581 tcaccaactg taatggttaa tccaaatggc acgccattac aatgtaactt aattccataa
     5641 ttatttttat ttgcgggcag gcaaaaacct gccccttgta tctacaatat tcaatgcaac
     5701 caattcaaat caaaccgctt tggctaaccg agctggctca agcctttaat aaccctatcg
     5761 acttattaca atttcttgaa ctcaatccaa atgattttga gggcgatata gctgcccgta
     5821 aattatttgc tcttcgagtg ccaagaatgt tcgccgaaaa aatggaaaag ggtaacccaa
     5881 acgatccgct ttttttgcaa gcaatgtctt tacaggcgga atttattgag gcagaagggt
     5941 ttgtggtaga tccattggaa gagcaacaaa gcccagcacc gaatatttta cataaatacc
     6001 ataaccgttt gttatttatg ctaaaaaaca gttgtgcgat taactgccgt tactgttttc
     6061 gccgtcattt cccttatgag gaagtgaaaa gcggtaaagc tgtgtggcaa cagggactaa
     6121 cttatattgc tgagcatccc gagctggagg aagtgattct gtccggtggt gatccgctga
     6181 tggcaaaaga tcaggactta gattggatat taacgcaatt agagcagatt tcgcatatca
     6241 aaaccctgcg tattcattca cgcttgccgg tcgttattcc caatcgaatt acgacagaat
     6301 tatgtgaacg gttatcaaaa tctcgcttga atgtcgtgtt ggttacccat attaatcacg
     6361 ccaatgaaat tgatgctgtt tttgcaaaca aaatggctca actgaaaaaa gccggcgttg
     6421 tattgctgaa tcaatcggtg atgttaaaag gtgtaaacga taatgctcaa accttaaaaa
     6481 ggttaagtga taaacttttt gaatatggga ttttgcctta ctatttgcat ttatttgata
     6541 aagtggcagg ggcgagccat ttctatattg aagatgaaca ggctggtgag atttaccgag
     6601 aattacagcg tatcacttca ggctatcttg tgccaaagct ggcaagagaa atagcgaaag
     6661 agccaaataa aattttgaaa ggttagctta aacagaaatc aagaaacaaa aggcgaaaat
     6721 ggtaaaattt tcgccttttt taaccgcttg ttgcaagcgg ttaaattctc ttactttttt
     6781 gcaaattaga agttcgcatt gcgtggagca cgtgggaatg ggattacatc acgaatgttt
     6841 tgtaacccgg ttacatacac gattaaacgc tcgaaaccta aaccgaaacc tgagtgtggc
     6901 actgtaccgt atttacgcaa atcacgatac caccagtaat cttcagggtt taagcccatt
     6961 tcattcatac gctgatctaa cacttctaaa cgttcttcac gttgagaacc accgataatt
     7021 tcaccgattc ccggagctaa aacgtccatt gcggcaaccg ttttgccgtc atcatttaaa
     7081 cgcatataga aggctttaat gtcttttgga tagtttttca cgaccaccgg cgatttaaag
     7141 tgttcttcgg ctaagaaacg ctcgtgttca gaggaaagat cgattcccca agacaccggg
     7201 aattcaaatt ctttgccgga tttcaataag atctcaatcg catcggtgta atctacttga
     7261 gcaaatggcg aattgataaa gttttctaaa cgactaatcg cttctttatc aacgtgttta
     7321 acgaaaaatt ccatatcatc tttacgttct gcaagcaccg ctttaaacac atatttcagc
     7381 atatcttcgg ctaattttgc attgtcgttt aagtcggcaa aggcaacttc cggctccatc
     7441 atccagaatt ctgcaagatg gcgagttgtg tttgagtttt ctgcacggaa tgtcggaccg
     7501 aaagtataaa ctttacttaa ggcacacgca taggtttccg catttaactg gccggatacg
     7561 gttaagaacg attctttacc gaagaaatct tggttaaaat ccactttgcc gttttcgcca
     7621 cgaggtagat tttctaaatc cagcgtagaa acacggaaca tttcacctgc accttcagta
     7681 tctgaggcgg taattacagg tgttgctacc caatagaaac cttgctcgtg gaagaaacgg
     7741 tggatagctt gagctaaaca gtgacgaaca cgagcaactg caccgattaa gttcgtacga
     7801 gggcgtaagt gagcgacttc acgcagatat tcgatagagt ggcgtttcgc tgccattggg
     7861 taggtatcag gatcttccac ccaaccaagc acttccactt tacttgcttg taattcaacg
     7921 gcttgacctt cagcagggga ttccacaact ttaccggtca ccaccacaga gcaacctgcg
     7981 gttaaatgta atacttcggc attataattt ggtagatcgt tattaataat cgcttgaatt
     8041 gggtcaaagc aagaaccatc gtaaacgctc aagaaagata aaccggcttt tgaatcacgg
     8101 cgggtacgca cccaaccacg cactgtcgct tcttcaccta ccgctatctt gccggatagg
     8161 atttccgaaa ctgttggaaa tttagacatt gaatacctca aattctttgt tgtgagtttt
     8221 aatataagaa acgttccgta ttctacaaga aaataggggg aaatataata attttac
//
LOCUS       DANLZC010000112         8061 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.112, whole genome shotgun sequence.
ACCESSION   DANLZC010000112 DANLZC010000000
VERSION     DANLZC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.112"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(52..1092)
                     /locus_tag="PXE44_001945"
     CDS             complement(52..1092)
                     /locus_tag="PXE44_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5589427.1"
                     /translation="MQTTLSPQQIAKKHRTIEKKRSFLLLFFLLICLLSLIFDIMTGP
                     SLLPFNEVWKALFQLGEIEETTKVIVYELRLPMAIMALVVGAALGVGGAEIQTLLNNP
                     MASPYTLGLAAAAGFGASIVIAFGGFGLPTEISVPVGAFVMTLFSSAILFAFSSLGRF
                     SSAILVLVGIALLFLFQSLLSLVQFISAPEVSQQILFWLFGNLTKATWQTISIAFGVT
                     TVCILLLFKDAWKLTALRLGEARALSLGINLTWLRMKTLLIVSVMTATCISFVGIIGF
                     IGLVAPHIARMLLGEDQRFFLPGSMLVGAAFLSMSSVLSKSLVPGALFPVGIITALVG
                     VQFFFWIVLAKR"
     gene            complement(1181..1771)
                     /locus_tag="PXE44_001946"
     CDS             complement(1181..1771)
                     /locus_tag="PXE44_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HDL5589428.1"
                     /translation="MNFLTHSLISLEIDTQTKQQTLYGNFAGDFYKGLIPHLNLADNL
                     KSGVVLHRSIDKISDRPDSLTTQLLQGTFGRYKGIVSDIMIDHFLAKHFQRLFAKDLN
                     EVQAQIFQQIEQQRTIFPEQFVPMFNWLQSRKALTHYADLDFLEQVFLGMSSRVKKGE
                     ILRNAVTEIWQNYTLLEEAAIQEFFTVKDRATRELS"
     gene            complement(1772..3145)
                     /gene="modF"
                     /locus_tag="PXE44_001947"
     CDS             complement(1772..3145)
                     /gene="modF"
                     /locus_tag="PXE44_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDL5589429.1"
                     /translation="MVVDGNGSGKTTFSLALQGKISLYSGDYGNSFQHIQLLSFEQQQ
                     KMLEQIFKDRNNDSVSPDDFGLTARQVILNGSPKEALCEEYAHKLHIQHLLERPFMHL
                     STGESRKVLFCQMLVSEPDLFILDEPFEGLDQQSVQYWQQLIAELQSKMALVLIVNRF
                     NDIPDAANHIELLDNLSLILQGERAEIEQQAVYSQLKFAEENVNVPLPDSAAPLLSLP
                     ENINPFELENVNIQYGEKKILDNLSWTVQPKQHWWIKGPNGAGKSTLLSIITGDHPQS
                     YANKVRLFGKQRGSGETIWDIKKNIGYVSSQLHMDYRVNCSAMEVILSGFFDSIGVYQ
                     QVPNALQLKAMEWLERLNLSNVAKKPFRSLSWGQQRLLLITRAMVKHPSILILDEPLQ
                     GLDGINRKLVKQFIEQLVTNSQTQLLFVSHQDSDAPNCITHLFEFVPKGETNEFGFEY
                     HQTALGA"
     gene            complement(3263..4216)
                     /gene="fmt"
                     /locus_tag="PXE44_001948"
     CDS             complement(3263..4216)
                     /gene="fmt"
                     /locus_tag="PXE44_001948"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL5589430.1"
                     /translation="MSKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGK
                     KLQASPVKQLAEAHNIPVYQPKSLRKEEAQAELKALNADVMVVVAYGLILPEAVLNAP
                     KYGCLNVHGSLLPRWRGAAPIQRSIWAGDTETGVTIMLMDVGLDTGDMLHKVTTPIEP
                     NETSASLYAKLAELAPLALLEVLNGLANHTFKAEKQDEALSNYAEKLSKEEAKLDWQL
                     PAVQLERNIRAFNPWPFSFLTLTVDGVEQSIKIHQATVLPHQEKAAGTVINVDKTGIQ
                     IATQQGVLNITQLQPAGKKPMSVQDFLNGRADWFVSGTQLS"
     gene            4331..4942
                     /locus_tag="PXE44_001949"
     CDS             4331..4942
                     /locus_tag="PXE44_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL5589431.1"
                     /translation="MEFFIPKADTIFEEEIKKSRFITYLRHTEGMEQAKAFWQELKIQ
                     HPHARHWCWATVAGLPNDSQQYGFSDDGEPSGTAGKPMLNYLLGSGLGEITAVVVRYY
                     GGIQLGTGGLVKAYGNGVQQALLQLERIRKVLRKNYRLQCEYEQFNTISHLISEKDIV
                     IVEQLFSDKVDLLLAFNPSEVEAFADELTQRFSGKLALIKEEI"
     gene            4946..6430
                     /locus_tag="PXE44_001950"
     CDS             4946..6430
                     /locus_tag="PXE44_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter"
                     /protein_id="HDL5589432.1"
                     /translation="MQFFSIIRIVGILVMCFSFTMLVPATVALIYGDGGGRAFLEAFA
                     LNFMVGTLLWWLCRDNKNELRSREGFLIVVLFWVVLGLLGAVPFIILENPDLNVAESI
                     FESFSGLTTTGATVITGLDSLPKAILFYRQFLQWLGGMGIIVLAVAIIPLLGIGGMQL
                     YRAEMPGPLKEQKMRPRIAETAKALWLIYLSLTILCAVAFWLAGMTVFDAISHSFSTV
                     SIGGFSTHDASIGYFNNAAISYITVFFLLLSACNFALHFAVIDRIRDAQSRKKGGILS
                     RLYWGDYEFRFFLMVQLGLFLVCTLVLVGYGYFDNTSVTIEQALFQSVSISTTAGFTT
                     NDFSQWSSFLPTLLVLSSFIGGCAGSTGGGLKVIRVLLLYLQSKREIHRYIHPNVIQP
                     IKLGNHVLSERVVDGIWAFFSAYFFVFVICWLATIACGMETFDALNAVIASLNNLGPA
                     LGSVSSNFTQVPDSAKLVLTFAMVCGRLEVFTLLVILSPVFWKD"
     gene            6431..6958
                     /gene="hemG"
                     /locus_tag="PXE44_001951"
     CDS             6431..6958
                     /gene="hemG"
                     /locus_tag="PXE44_001951"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDL5589433.1"
                     /translation="MKTLILYFTTDGQTKKIAERLADVITHEVELISLKDQAVDFAEK
                     IANADQIVIGASIRYGHFNPLVYQFVEQHYPALNAKKTAFYGVNLTARKDNRNTPETN
                     TYVRKFLAKVKWKPTHIEVIAGALLYLRYKPFDRLMIRFIMKITKGETDTSKEYEYTD
                     WQQVENFGKLLANQL"
     gene            7090..7485
                     /gene="mscL"
                     /locus_tag="PXE44_001952"
     CDS             7090..7485
                     /gene="mscL"
                     /locus_tag="PXE44_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL5589434.1"
                     /translation="MSILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPP
                     IGWLIGGVDFKDLAIEIAPAKEGAEAVMLKYGAFIQNVFDFLIIALAVFGMVKVINSL
                     KKAPVVEEAPAEPTAEEKLLTEIRDLLKK"
     gene            complement(7598..7861)
                     /gene="rpsT"
                     /locus_tag="PXE44_001953"
     CDS             complement(7598..7861)
                     /gene="rpsT"
                     /locus_tag="PXE44_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL5589435.1"
                     /translation="MANIKSAKKRAVQSEKRRQHNASQRSMMRTFIKKVYAAVAAGDK
                     AAAQTAFVEMQKVVDRMASKNLIHANKAANHKSKLAAQIKKLA"
BASE COUNT         2231 a         1607 c         1802 g         2421 t
ORIGIN      
        1 taagtttcca cgcttaatag ttacatcgtt taagttgagc atagaaaatc cttatcgttt
       61 cgccaataca atccagaaaa agaactgcac accgactaat gccgtaataa taccgaccgg
      121 aaataatgcg cccggcacaa ggctttttga tagcacggat gacatcgata aaaatgccgc
      181 acccactaac attgaaccgg gcaggaaaaa gcgttggtct tcgcctagca acattcgggc
      241 aatatgcggg gcaactaagc caataaagcc gataatgcct acaaagctaa tgcaggtggc
      301 cgtcattaca gacacaatca gcaatgtttt cattcgtaac caagttaagt taatgcctaa
      361 gctcaatgct cgagcttcgc ccaaacgcaa ggcagttaat ttccacgcat ctttgaataa
      421 taataaaata cagactgtag ttacgccaaa ggcaattgag atagtctgcc aagtagcttt
      481 agttaaatta ccgaatagcc aaaacagaat ttgttgggat acctcaggtg cagaaataaa
      541 ttgcaccaat gacagtaaag actgaaataa aaacaataag gcaattccta ccagcactaa
      601 tattgccgag ctaaaacgtc ctaaagagga aaaggcaaat aaaatggcgg aagaaaatag
      661 tgtcatcaca aaagccccga caggtacaga aatttcggtg ggtaaaccaa atccaccaaa
      721 ggcaatcaca atcgaagcac caaagccggc tgcagctgct aaacccaatg tataagggct
      781 tgccattgga ttattaagca aagtttgaat ttcagctccg ccaacaccaa gtgctgctcc
      841 aaccaccaat gccattattg ccattggtaa gcgtagctca tagacaatca ctttggtggt
      901 ttcttcaatt tcgcctaatt ggaataaggc tttccaaact tcattaaacg gaagcaggga
      961 agggcctgtc ataatatcga aaatgaggct gagtaaacaa atgagtaaga aaaagagtaa
     1021 taaaaaactg cgtttctttt caatagttcg gtgcttttta gctatttgtt ggggagagag
     1081 cgttgtttgc ataaaaatgt aatgtttgag aattattatc agacaggata agagaaaaga
     1141 tcgataaatt caagaatatc caagataccg actaaagtat tcaactaagt tctcgagtag
     1201 ccctgtcttt tacagtaaaa aactcctgaa tagcggcttc ttctaaaaga gtataattct
     1261 gccaaatttc ggttacagca tttcttaaaa tctcaccttt cttaactctt gatgacattc
     1321 ccaagaatac ctgttctaag aaatctaaat cggcataatg agttaatgct tttcgtgact
     1381 gtaaccagtt aaacatcggc acaaattgtt caggaaaaat cgtgcgttgt tgttcgattt
     1441 gctgaaagat ctgtgcttgt acctcattta aatctttagc aaataaccgt tggaaatgtt
     1501 tagcgagaaa atggtcgatc ataatatccg acacaatgcc tttataacgc ccgaatgtgc
     1561 cttggagtaa ttgggttgtg aggctgtccg gtcggtccga aatcttatca atcgagcggt
     1621 gtaacaccac accgcttttt aagttatccg ctaaattgag atgcggaatt aaacctttat
     1681 agaaatcacc ggcaaagttg ccatataggg tctgttgctt ggtttgggtg tcaatttcaa
     1741 gtgaaatcag cgaatgggta agaaaattca tttatgctcc aagtgcagtt tggtgatact
     1801 caaacccaaa ctcgttggtt tcgcctttcg gtacaaactc aaataaatgc gtaatgcaat
     1861 ttggagcatc gctgtcttgg tgcgaaacaa aaagtagttg cgtttggctg ttagtcacta
     1921 attgttcaat gaactgtttg actaatttgc ggttaatgcc gtctaaacct tgtaacggtt
     1981 cgtctagaat cagaattgat gggtgtttca ccattgctcg ggtaatcaaa agtaagcgtt
     2041 gttgccccca cgagagagag cgaaacggct ttttggcaac attgcttaaa ttgaggcgtt
     2101 ccaaccattc cattgctttt aattgcaagg cattcggcac ttgttgatac acgccgatgc
     2161 tgtcaaaaaa gccagaaaga atgacctcca ttgcggagca atttacccga taatccatat
     2221 gcaactggct gctgacatag ccgatatttt tcttaatatc ccaaatggtt tcgccacttc
     2281 cccgttgttt gccaaatagt cggactttat tggcataaga ttgtgggtga tcgccggtga
     2341 taatcgaaag caaggtcgat ttcccagctc cattcgggcc tttaatccac cagtgttgtt
     2401 taggctgaac cgtccaactt aggttatcta aaatcttctt ctccccatat tgaatattca
     2461 cattttccaa ctcaaacgga ttgatgtttt ccggcaggct gagtagcgga gctgcactat
     2521 caggcagtgg cacattgaca ttctcttctg caaattttag ctgagaatag accgcttgtt
     2581 gttcaatttc tgctcgctct ccttgcagaa ttaatgataa attatctaat aactcaatat
     2641 gattggcagc atcaggaata tcgttaaaac gatttacaat cagcactaac gccattttgc
     2701 tttgtagttc ggcaatcagt tgttgccaat attgcaccga ctgctgatcc agcccttcaa
     2761 acggttcgtc taaaatgaat aaatccggct cgctcactaa catttggcaa aatagcactt
     2821 tgcggctttc accggtggaa aggtgcataa acgggcgttc aagcagatgt tgaatatgca
     2881 atttgtgggc atattcctca cataacgcct cttttggact gccatttaaa attacttgtc
     2941 gtgcggttaa gccgaaatca tcgggcgaga cactatcgtt gttccgatct ttgaaaatct
     3001 gctccaacat tttttgttgt tgctcgaaag agaggagctg aatatgttgg aacgaattac
     3061 catagtcgcc ggaatagagc gagattttac cctgtaaagc gagtgaaaaa gttgttttgc
     3121 ctgaaccgtt tccgtctacg accacctaaa agtcattggg ttggatctgt aaggcgtcaa
     3181 tcgtgagttt atgttgttta accagtgaaa atcgggcatt ggttattgta agtgaaggct
     3241 gtgtttgcat atatttcccg ttttaactta gttgtgtccc gctgacgaac caatccgctc
     3301 gaccgtttaa gaaatcttgc accgacattg gttttttacc cgccggttga agttgggtaa
     3361 tattcagcac gccctgttgg gtggcaattt gaatcccggt tttatccaca ttaattaccg
     3421 tgccggctgc tttttcttgg tgaggtaaca ccgtggcttg gtgaattttg atactttgct
     3481 caacgccatc aacggttaag gttaaaaaac taaacggcca agggttaaaa gctcggatat
     3541 tgcgttccag ttgaactgct gggagttgcc aatccaattt agcttcttct ttggaaagtt
     3601 tctcggcgta attggacagt gcttcatctt gtttttccgc tttgaatgtg tggttggcta
     3661 aaccgtttaa cacctcaagc aatgccagag gggcaagttc tgccagtttg gcatacaagg
     3721 aggcggaggt ttcattcggc tcaatcgggg ttgtgacttt atgtaacata tcaccggtgt
     3781 ctaagccaac gtccattagc ataatggtta cgccggtttc tgtatcacct gcccaaatcg
     3841 aacgctgaat cggagctgct ccacgccagc gaggtagtag cgaaccgtgt acgtttaggc
     3901 aaccatattt aggagcattc aatactgctt caggtagaat taagccgtaa gctaccacca
     3961 ccatcacatc ggcatttagg gctttcagtt cagcttgagc ctcttcctta cgcagggatt
     4021 tgggttggta aacaggaatg ttgtgagctt cggccagctg ttttaccggg ctggcttgca
     4081 gttttttgcc tcgcccggca ggtttatccg gctgagtata aaccgcaatc acgttatgct
     4141 cggaatctaa taaggcttgt aaatgttgag cggcaaaatc aggcgtgccg gcaaaaataa
     4201 tattgagttt tgacatattt tttattcgtt caagttatca atgaaagact tattaaacca
     4261 ttaaacagca caaataaaaa gtggcttgaa tatggtatca ttgccacatt caagataaga
     4321 gaatcaaaca atggaatttt ttatccccaa agcggataca atttttgaag aagagattaa
     4381 aaaaagccga tttatcacct accttcgcca tactgagggt atggagcagg ccaaggcatt
     4441 ttggcaggag ctgaaaattc agcatccgca cgcacgccat tggtgttggg caacggtggc
     4501 aggcttgccg aatgattcgc aacaatacgg cttttcggat gatggagaac cgtctggtac
     4561 agcaggtaag ccaatgctca attatttgct tgggtcaggc ttgggggaaa ttacagcggt
     4621 ggtggttcgc tactacggtg gtatccagct tggcaccggc ggcttggtga aagcctatgg
     4681 caatggggtg caacaggcgt tattgcagtt ggagcggatt cgcaaagttt tgcgaaaaaa
     4741 ctaccgcttg caatgtgaat atgagcagtt taataccatt tctcatctca ttagcgagaa
     4801 agatattgtg attgtcgagc aactttttag cgataaagtg gatttgttgc tggcatttaa
     4861 cccgagcgag gttgaggcct ttgcagatga attaacccaa cgcttttcgg gcaagctcgc
     4921 attaataaaa gaagaaatct agtctatgca atttttttcc attattcgta ttgttggcat
     4981 tttggtgatg tgtttttcat tcacaatgct tgtgccggca acggtcgccc taatttatgg
     5041 cgatggcggt gggcgggcgt ttttagaagc cttcgctctg aattttatgg tcggtacgct
     5101 gctttggtgg ctgtgccgtg acaacaaaaa cgagctacgt tcccgtgagg gctttttgat
     5161 tgtagtactg ttttgggtgg tgctgggttt gctcggggct gtgcctttta ttattttgga
     5221 aaaccccgat ttaaacgtgg cggaatccat ttttgaatcc ttttccggct taactacaac
     5281 gggtgcaacc gtcatcaccg gcttagatag cttgcccaaa gcgattttat tctaccgcca
     5341 atttttgcaa tggctagggg gaatggggat cattgtgtta gcggttgcga ttattccgct
     5401 gttaggcatt ggtggaatgc aactttatcg tgcggaaatg cccggaccgc tcaaagagca
     5461 gaaaatgcga cctcgcattg cggaaacggc aaaagcgttg tggctgattt atctcagcct
     5521 gacgattctg tgtgcggtcg cattttggct cgccggaatg acggtgtttg atgccatttc
     5581 gcatagcttt tccacggttt cgattggcgg attttctacc cacgatgcca gcatcggcta
     5641 ttttaataat gcggcaatta gctatattac cgtgtttttc ctgctacttt ctgcctgcaa
     5701 ctttgcattg cattttgcgg tgattgatcg catacgagat gcacaatcac gcaaaaaagg
     5761 gggtatttta agccgcttgt attggggcga ttacgagttt cgttttttct tgatggtgca
     5821 gttagggctt ttcttggttt gtaccttggt gttagtgggt tacggttatt tcgataatac
     5881 ctctgttacg attgagcaag cgttgtttca atcggtgtcc atttccacca cggccggctt
     5941 tactaccaat gattttagcc aatggagttc gtttttaccc acgctgttgg tgctgtcttc
     6001 atttatcggt ggttgtgccg gctcaaccgg tggcggcttg aaagtgattc gggtgttgct
     6061 gctttattta caaagtaaac gtgaaatcca ccgctacatt caccccaatg tgattcaacc
     6121 gattaaatta ggcaaccacg ttttatcgga acgggtggtt gatggtattt gggcgttttt
     6181 ctcggcgtac tttttcgtgt tcgtgatctg ctggcttgct accatcgctt gtggaatgga
     6241 aacctttgat gctctaaatg cggtaattgc ctcactaaat aatctaggtc ctgcattagg
     6301 gagcgtgagc agcaatttca cccaagtgcc ggacagtgcc aagttagtgc tgacttttgc
     6361 aatggtatgc ggacgtctgg aagtgtttac cttgctggtg attttaagcc ctgttttttg
     6421 gaaagattaa atgaaaacct taattctcta ttttacgacc gatggtcaaa ccaaaaaaat
     6481 tgccgaacga ttggcggacg tgattactca cgaagtcgag ctgatttcgc taaaagatca
     6541 agcggtcgat tttgccgaaa aaattgcaaa tgccgaccaa attgtcatcg gtgcttcaat
     6601 tcgctatggg cattttaacc cgttggttta tcagtttgtg gaacagcatt atccggcatt
     6661 aaacgccaag aaaactgcct tttacggtgt gaatttgacc gctcgcaaag ataatcgaaa
     6721 cacgcctgaa accaatactt atgtgcgtaa atttttagct aaagtgaaat ggaaaccaac
     6781 ccacattgag gtaattgctg gggcattact ttacctacgc tataagccat tcgaccgatt
     6841 gatgattcgc tttattatga aaattaccaa aggcgaaacc gatacctcca aagaatatga
     6901 gtacaccgat tggcagcagg tggagaattt tggcaaatta ttagccaatc agttataatc
     6961 aaactgatct aaatcaaaaa aatgtaaatt tatttaaatg ttgtagaaat tttaaagatt
     7021 ttgattataa tcgccagccc acgttcttgc gaagaccgtg tttaatcatt gaatgaaagg
     7081 aaagccccct tgagtatttt aaaagagttc cgtgaatttg cggtaaaagg taatgttgtt
     7141 gatatggctg tcggtgtgat tatcggcggt gcattcggta aaattgtttc ttcattagtt
     7201 agcgatgttg taatgccacc tatcggttgg ttaatcggtg gcgtggattt caaagattta
     7261 gccattgaaa tcgccccggc aaaagaaggt gcggaagcag tgatgttaaa atacggtgca
     7321 tttatccaaa acgtatttga tttcttaatt atcgcacttg cggtattcgg tatggtgaaa
     7381 gtgattaact cactgaaaaa agctccagtt gtagaagaag ctcctgcaga gccaacagcg
     7441 gaagagaaat tattaactga aatccgtgat ttattgaaaa aataattaac tgattttgaa
     7501 acaagggcta ttttagaata tctagaatag ccctttgtgt tatggttaaa aagaaaaaaa
     7561 ccaacataaa gtcggttttt ttcatatctg ttcgatatta cgctaatttt ttgatttgcg
     7621 ctgctaattt agatttgtgg ttagccgctt tgttagcgtg gattaagttt ttagatgcca
     7681 tacggtctac aactttttgc atttcaacga aagccgtttg agctgctgct ttatcgcctg
     7741 ctgctactgc tgcatatact tttttgatga atgtacgcat cattgagcgt tggcttgcgt
     7801 tgtgttggcg gcgtttttca gattgaaccg cacgtttttt tgctgacttg atattagcca
     7861 aggtcaaact cctaaaatat ctgtgtaaaa attagatata aacaaaatcg ttgtttatat
     7921 tgcctatcga tagtttttaa ttcattttca acgagacacg tgcttgaaaa taacaccgtt
     7981 tttctgatag aaaacgatag ggcgagattt taacagtttt tttctgattt tcctataaca
     8041 aactgctaaa attatgatcg a
//
LOCUS       DANLZC010000113         7938 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.113, whole genome shotgun sequence.
ACCESSION   DANLZC010000113 DANLZC010000000
VERSION     DANLZC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.113"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..418)
                     /locus_tag="PXE44_001954"
     CDS             complement(<1..418)
                     /locus_tag="PXE44_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL5589436.1"
                     /translation="MSNILNWPDYKVLQVSELEHDYQVHAEVSEPPTQCPHCNHPEIV
                     GFGRRDEVIMDTPVHGRRTGIMLNRRRYRCQSCRKTFLEPVPHKDEKRQMTNRLIQYI
                     ERESLRRTFSSVAEDVGVDEKTVRNIFNDYCERLEKT"
     gene            complement(418..720)
                     /locus_tag="PXE44_001955"
     CDS             complement(418..720)
                     /locus_tag="PXE44_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589437.1"
                     /translation="MKIKLYFPDESIATIKRMGLRQMSPETLRWTADHPSSSYGMGAL
                     LRGKSGEILDGKSFAAMVHAFGAWIETDSEDTSRRVHNALVTAATGTEESVKVAKE"
     gene            complement(761..949)
                     /locus_tag="PXE44_001956"
     CDS             complement(761..949)
                     /locus_tag="PXE44_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589438.1"
                     /translation="MSYDANDALNEIEEALSELERVAEDLINNNPNKESELRGQGVHQ
                     ATKHLRFRIRNIRRGEAI"
     gene            1168..2724
                     /locus_tag="PXE44_001957"
     CDS             1168..2724
                     /locus_tag="PXE44_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HDL5589439.1"
                     /translation="MSLIPHIHIAYGSESGNAEKLAQQLAIQPFLLPYSPTLSTLNET
                     DLSQLSAESLLLIVTSSFGDGEPPENADEFAEKLENLTACNLQYAIFGLGDITYDKFC
                     GYSKQLDSLLQQKQAKAVIARVDADLNYQEIFKQWLPLVEQALHSTNKETIQHSLSVQ
                     AYGEEMAYQAEVLEIKHLANSSPAVYHIRLSLKESGIFYQAGDLIYVKVQQPDRLLNE
                     YAEWFNDQNAVALLRHKELRLLSKNVLRDIQKICGSNELKDLTKISNKKALEQYVYGR
                     DLLDVLRDFDPNKSVTLADLEPILSNLAARAYSISSCGKTHPDYVDLCIRHVSYHLNG
                     RDYQGTASGYLSELQAGDFVSIFANANPNFRLPETLTAPVVMIGSGTGIAPHIAFLQA
                     LEQQYQGVESYLFFGERYRDKDFLYQTELENYLAKGTLTQLFTAFSRDQAEKFYVQDA
                     LSQQAELVWHLIQQGAYFYICGSKAMSKAIDAELIKIADQIGGDPYIDDFNNIIAKLV
                     AEGRLMRDVY"
     gene            complement(2782..3039)
                     /gene="rpsQ"
                     /locus_tag="PXE44_001958"
     CDS             complement(2782..3039)
                     /gene="rpsQ"
                     /locus_tag="PXE44_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL5589440.1"
                     /translation="MTDKIRTVQGKVVSDKMDKSFVVAIERTVKHPLYGKFIRRTTKL
                     HVHDENNEAKLGDVVEIKECRPISKTKSWTLVRVVEKAVVA"
     gene            complement(3039..3230)
                     /gene="rpmC"
                     /locus_tag="PXE44_001959"
     CDS             complement(3039..3230)
                     /gene="rpmC"
                     /locus_tag="PXE44_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL5589441.1"
                     /translation="MKAQELRNKSVEELNGELVNLLGEQFKLRMQAATGQLQQTHQLK
                     QVRRSIAQVKTVLTEKAGE"
     gene            complement(3230..3640)
                     /gene="rplP"
                     /locus_tag="PXE44_001960"
     CDS             complement(3230..3640)
                     /gene="rplP"
                     /locus_tag="PXE44_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL5589442.1"
                     /translation="MLQPKRTKFRKVHKGRNRGIAGGTEVSFGSFGLKAIGRGRLTAR
                     QIEAARRAMSRAVKRQGKIWIRVFPDKPITEKPLEVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVSEEVARNAFALAAAKLPFKTTFVTKTVM"
     gene            complement(3654..4361)
                     /gene="rpsC"
                     /locus_tag="PXE44_001961"
     CDS             complement(3654..4361)
                     /gene="rpsC"
                     /locus_tag="PXE44_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL5589443.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTQDFADNLDGDFKVRKFLN
                     KELANASVSRITIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRNAVAKIAGVPAQIN
                     IAEVKKPELDAKLVADSIASQLERRVMFRRAMKKAVQNAMKLGAKGIKVEVSGRLGGA
                     EIARSEWYREGRVPLHTLRADIDYSTAEAHTTYGVIGVKVWIFKGEILGGMAAVIESE
                     KEPAAQPKKAPRGKGRK"
     gene            complement(4381..4713)
                     /gene="rplV"
                     /locus_tag="PXE44_001962"
     CDS             complement(4381..4713)
                     /gene="rplV"
                     /locus_tag="PXE44_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL5589444.1"
                     /translation="METIAKHRYARTSAQKARLVADLIRGKKVSVALEILTFTNKKAA
                     TLVKKVLESAIANAEHNDGADVDDLKVAKIFVDEGPSMKRVMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(4724..4999)
                     /gene="rpsS"
                     /locus_tag="PXE44_001963"
     CDS             complement(4724..4999)
                     /gene="rpsS"
                     /locus_tag="PXE44_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL5589445.1"
                     /translation="MPRSLKKGPFLDLHLLKKVEKAVESGDKKPIKTWSRRSMIIPSM
                     IGLTIAVHNGRQHVPVYVSDEMIGHKLGEFAPTRTYRGHAADKKAKK"
     gene            complement(5030..5851)
                     /gene="rplB"
                     /locus_tag="PXE44_001964"
     CDS             complement(5030..5851)
                     /gene="rplB"
                     /locus_tag="PXE44_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL5589446.1"
                     /translation="MAIVKCKPTSAGRRHVVKVVNAELHKGKPFAALLDTKSKTGGRN
                     NLGRITTRHIGGGHKQHYRLIDFKRNKLDIPGVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLTAGDQIQAGASAPIKVGNALPMRNIPVGTTVHNVELKPGKGGQIARSAGA
                     YVQIIAREGNYVTLRLRSGEMRKVLAECTATIGEVGNSEHMLRVLGKAGANRWRGIRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRHNKRTDKFIVRRRGK"
     gene            complement(5870..6175)
                     /gene="rplW"
                     /locus_tag="PXE44_001965"
     CDS             complement(5870..6175)
                     /gene="rplW"
                     /locus_tag="PXE44_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL5589447.1"
                     /translation="MIQQERLLNVLKAPHISEKATNNAEKGNTIVFKVALDANKVEIA
                     NAVEELFEVKVDSVRTVVVKGKTKRRGAKVGRRSDWKKAYVTLQEGQSLDFVEGAAE"
     gene            complement(6172..6774)
                     /gene="rplD"
                     /locus_tag="PXE44_001966"
     CDS             complement(6172..6774)
                     /gene="rplD"
                     /locus_tag="PXE44_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL5589448.1"
                     /translation="MELQVVGANALTVSETTFGREFNEALIHQVVVAYAAGARQGSRA
                     QKTRAEVSGSGKKPWRQKGTGRARSGDIKSPIWRSGSVTFAAKPQDHSQKVNKRMYRG
                     AIKSILSELVRQDRLVVVEKFEIDAPKTKVLAQKLKDMALNDALIITANVDENLFLAA
                     RNLYKVDVRDVQGIDPVSLIAFDKVVVTVDAVKQIEEMLA"
     gene            complement(6790..7419)
                     /gene="rplC"
                     /locus_tag="PXE44_001967"
     CDS             complement(6790..7419)
                     /gene="rplC"
                     /locus_tag="PXE44_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL5589449.1"
                     /translation="MIGLIGRKVGMTRVFTEDGVSIPVTVIEIEANRVTQVKTLENDG
                     YSAIQVTTGSKKASRVTKPEAGHFVKAGVEAGRGLWEFRTTEGEEFTLGQEINVDIFT
                     DVKKVDVTGTSKGKGFQGGVKRWNFRTQDATHGNSLSHRVLGSIGQNQTPGRVFKGKK
                     MAGHLGAERVTVQSLEVVRVDVERKLLLVKGAVPGATNGDVIVKPAVKA"
     gene            complement(7445..7756)
                     /gene="rpsJ"
                     /locus_tag="PXE44_001968"
     CDS             complement(7445..7756)
                     /gene="rpsJ"
                     /locus_tag="PXE44_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL5589450.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQSTAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHKRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
BASE COUNT         2195 a         1768 c         1554 g         2421 t
ORIGIN      
        1 gcgtcttttc gaggcgttca caatagtcgt tgaaaatgtt acggacggtc ttttcatcga
       61 cgcccacatc ctcggcaaca ctggagaacg tgcggcgcag gctctcgcgc tcgatgtact
      121 ggatcaggcg gttggtcatc tgccgctttt cgtctttgtg cggtacaggc tccagaaacg
      181 tctttcggca agactggcag cgatagcgac ggcggttaag catgatgccg gtacgccttc
      241 cgtggacggg tgtatccatg atcacctcat cacggcgacc aaagccgacg atttcaggat
      301 gattgcagtg ggggcactgc gtaggcggtt cggaaacttc ggcgtgtacc tggtaatcgt
      361 gctccagttc ggatacctgg agcactttgt aatcaggcca gttgaggata ttcgacatta
      421 ctcctttgcc accttaaccg attcctcggt gccagtggca gcagtgacta gcgcgttatg
      481 aactcgacga ctggtatctt cactgtctgt ttcaatccac gcgccaaagg catgcaccat
      541 tgcagcaaaa gacttcccat caaggatttc accagacttg cctctgagca aagcgcccat
      601 accatagctg cttgacggat gatcggcagt ccacctgaga gtctcgggag acatttggcg
      661 aagccccatt cgctttatcg tggcgataga ctcgtccgga aaatagagtt tgattttcat
      721 gctgacctcg ctgaaagccg cccctagggc ggcatgttag tcaaattgct tcgccgcggc
      781 ggatgttgcg aatacgaaaa cggaggtgct ttgtcgcttg gtgaacccct tggccgcgta
      841 attctgattc tttattcggg ttgttgttga ttaggtcttc agcaaccctt tcaagctcgc
      901 taagcgcctc ttcgatttca ttcagcgcat catttgcatc gtatgacatg gcttctatcc
      961 tgcaccaagt tcggcggtgc gcccgttggg cttatgcccg agccgttgtt ctcagctctt
     1021 gaatctaata ttagggcatc tggccttttc cgtcaaccct aaaatccggt ttatttacaa
     1081 aattacgtct agcgcagatc aaccgctaaa tccggatacc caagatgatc tcttttacac
     1141 gcaatttttt attttcgagg agtttgtatg tctctcattc cccatatcca cattgcttat
     1201 ggttcggagt ctggtaacgc agaaaagctt gcccaacaat tagctatcca acccttttta
     1261 cttccctact cacccacact ttctacatta aacgaaaccg atttaagcca attatctgcc
     1321 gagagtttat tgcttattgt aaccagctcc tttggtgatg gcgaaccacc tgaaaacgcc
     1381 gatgaatttg cagaaaaatt ggaaaatttg accgcttgta atctgcaata tgcgatattc
     1441 gggctaggtg atattactta tgataaattc tgtggctata gcaaacaact agattcatta
     1501 ttacaacaaa aacaagctaa agctgtgatt gcaagggttg atgccgattt aaactatcaa
     1561 gaaattttta aacaatggct acccctagtg gagcaggcat tacattcaac aaacaaagaa
     1621 acgatccaac attccctttc tgttcaagct tacggcgagg aaatggcata ccaagcagaa
     1681 gtgttggaaa ttaagcattt ggcaaactca agccctgcgg tgtatcacat tcgtttatca
     1741 ttaaaagaga gcggtatttt ctatcaagca ggggatttaa tttatgtcaa agtgcagcaa
     1801 ccagacagac tattaaatga atatgcagaa tggtttaacg atcaaaatgc agttgcatta
     1861 ctacgccata aagaactccg actactaagt aaaaatgtac tgcgtgatat tcaaaaaatt
     1921 tgcggcagta atgaactcaa agatttaact aaaatttcca ataaaaaagc attggaacaa
     1981 tatgtctatg ggcgggattt attagacgta ttacgcgatt ttgacccaaa caaaagcgtg
     2041 acattagccg atttagagcc aattttatct aacttagccg cacgagccta ttcaattagc
     2101 tcttgcggta aaactcaccc tgattatgtt gatttatgta tccgccacgt ttcgtatcat
     2161 ttaaacggta gagattatca agggactgca agtgggtatt tatccgaatt acaagcgggc
     2221 gattttgtct cgatttttgc aaatgccaat ccgaatttcc gtttacccga aacattaacc
     2281 gctccggtgg tgatgattgg ctcaggtact ggcattgctc cgcatatcgc atttttacaa
     2341 gcgttagagc agcaatatca gggggttgaa agctatttat tcttcggcga gcgttatcgt
     2401 gataaagatt ttctgtatca aacggaactg gaaaattact tagcaaaagg caccttaact
     2461 cagctgttta ctgctttttc acgtgatcag gcagagaagt tttatgtgca agatgcccta
     2521 tcgcaacaag cagagttagt ttggcattta attcagcaag gggcttattt ctatatttgt
     2581 ggtagcaaag caatgagtaa agcaatagat gcggaactca tcaaaattgc agatcaaatt
     2641 ggaggcgatc cttatattga tgatttcaat aatattattg caaaattggt cgcagaaggc
     2701 cgattaatgc gagatgtata ttagcaaatg aaaaaaacta aaaacccagc aaaagctggg
     2761 tttttattta atcatagttc attaagcaac aactgctttc tcaactacac gaactaaagt
     2821 ccaagattta gtttttgaaa taggacgaca ttccttaatt tcaacaacat cacctaattt
     2881 agcttcattg ttttcatcgt gtacatgaag tttagtagta cgacggataa acttaccgta
     2941 taacggatgt ttaactgtac gttcgatagc aacaacaaat gatttatcca ttttgtcgct
     3001 aaccacttta ccttgtactg tacgaatttt atcagtcatt attcacccgc cttttcggtt
     3061 aatacagttt taacttgtgc aatactacga cgcacttgtt ttaactggtg ggtttgttga
     3121 agctgaccgg tggctgcttg catacgcaat ttgaattgtt cacctaaaag gttcactaac
     3181 tcaccattca gctcttcaac acttttatta cgtagttctt gagctttcat tacatcaccg
     3241 tcttagttac aaatgtggtt ttgaacggaa gtttagctgc cgctaatgca aacgcattgc
     3301 gtgcaacttc ttcagaaaca ccatccattt catagagaac tttacccggt tggattaagg
     3361 ctacccagta ctcaacgtta cctttacctt tacccatacg gacttctaat ggtttttcag
     3421 taattggttt gtctgggaac acacggatcc agattttacc ttgacgttta accgcacggc
     3481 tcatcgcacg acgagcagct tcaatttgac gagctgttaa acgaccacgg ccaatagctt
     3541 ttaaaccaaa tgaaccgaag ctcacttctg tgccacccgc gataccacgg ttacggcctt
     3601 tgtgtacttt acggaatttt gtgcgttttg gttgtaacat tcagtgattc tccttattta
     3661 cgacctttgc cacgtggcgc ctttttaggc tgtgccgctg gttctttttc agattcaatc
     3721 actgcagcca ttccaccaag aatttcacct ttgaagatcc acacttttac gccgattacg
     3781 ccgtaagtag tgtgagcttc agcagtacta taatcgatgt ctgcacgaag cgtatgtaga
     3841 ggtacacgac cttcacgata ccattctgag cgagcaattt ctgcaccacc taaacgaccg
     3901 ctaacttcaa ctttgatacc tttagcacct aatttcatcg cgttttgaac tgcttttttc
     3961 attgcacgac ggaacatcac acgacgttca agttgagaag cgatgctatc tgcgactaat
     4021 ttcgcatcta gctctggttt tttcacttca gcgatgttga tttgagctgg aacgccagcg
     4081 attttcgcaa ctgcgttgcg taatttttca acatcttcac cttttttacc gataacgata
     4141 cccgggcgag ctgtgtgaat agttacacga atgctttttg caggacgctc aatagtgatg
     4201 cgtgatacag aagcgttcgc taactctttg tttaaaaatt tacgtacttt gaagtcgccg
     4261 tctaagttat cagcgaaatc ttgtgtattc gcgaaccaag tagagttcca aggtttaaca
     4321 atacctaggc gaataccatt aggatgtact ttctgaccca tttgttattc tcctacttaa
     4381 ttaacgatct gacacaacca cagtgatgtg gcttgtacgt tttaaaatac gatctgcacg
     4441 accttttgca cgtggcatta cacgtttcat gcttggacct tcatcaacga agattttagc
     4501 aactttaaga tcatcgacat ctgcaccgtc attatgctct gcgtttgcaa tagctgactc
     4561 taatactttc ttaacaagcg tcgctgcttt tttgttagtg aaagttaaga tttctaatgc
     4621 tacagaaact tttttaccgc gaattaaatc agcaactaag cgagcttttt gggcagaagt
     4681 acgagcgtaa cgatgtttag caatagtttc catcttttac ctcttatttc ttagctttct
     4741 tatctgcggc gtgaccgcgg tatgtacgag tcggtgcaaa ttcacctaat ttatgaccga
     4801 tcatttcgtc ggaaacataa acaggaacgt gctgacgacc attatggact gcgatggtca
     4861 aaccaatcat tgatggaatg atcattgaac gacgggacca agttttaatt ggttttttat
     4921 ccccgctttc caccgccttc tctaccttct tcaacaagtg taggtcaagg aaaggacctt
     4981 tcttgaggga acgtggcatt ggtgtttacc ttttagttaa tgaattaaat tatttgccac
     5041 gacgacgtac gatgaattta tcagtacgtt tgttgtgacg agttttctta cctttggttt
     5101 gaacgcccca aggtgtaaca ggatgtttac cgaagttacg accttcacca ccaccgtgtg
     5161 ggtgatctac cgggttcatt gcagtaccac gaacagttgg acgaatacct ctccaacggt
     5221 ttgcacctgc tttaccaaga acgcgaagca tatgctctga gttacctact tcaccaatgg
     5281 ttgcagtaca ctcagctaat actttacgca tttcgcctga acgaagacgt aaagttacat
     5341 agttaccttc acgggcaatg atttgaacgt aagcaccagc agaacgtgca atttgaccac
     5401 ctttaccagg ttttaattca acgttgtgta cagttgtacc tactgggatg ttacgcattg
     5461 gtaatgcgtt acctacttta atcggagctg aagcacccgc ttgaatttga tcaccagcag
     5521 ttaaaccttt tggtgctaaa atatagcgac gttcaccatc tttataaagt actaatgcga
     5581 tattcgcaga acgatttgga tcgtattcta aacgctcaac aacacccgga atgtctaatt
     5641 tattacgttt aaaatcaatt aaacggtagt gttgtttgtg accgccacca atgtgacgag
     5701 ttgtgatacg acctaagttg ttacgaccac cagttttaga tttagtatct aaaagtgctg
     5761 caaaaggttt acctttgtga agctcagcgt ttacaacttt aacaacgtga cgacgaccag
     5821 cggaggtcgg cttacattta acgatagcca tttattttat ctcctctgat tactctgccg
     5881 caccttcaac gaagtcaagc gattgacctt cttgaagagt tacataagct tttttccagt
     5941 cactacgacg accaacttta gcaccacggc gtttagtttt acctttaact actacggtac
     6001 gaacagaatc aacttttact tcaaaaagtt cttcaactgc atttgcaatc tcaactttgt
     6061 ttgcatctaa tgcaactttg aatacgattg tattgccttt ttctgcgttg tttgtcgctt
     6121 tttcagagat atgtggtgct ttaagcacgt ttagcaaacg ttcttgttga atcatgctaa
     6181 catctcctca atttgtttta ctgcatctac ggttacaacc actttatcga aagcaattaa
     6241 gctaactggg tcaatacctt gcacatcacg cacatcaact ttatataagt tgcgtgctgc
     6301 taagaataaa ttctcatcta cgtttgcagt aatgataaga gcgtcattta acgccatatc
     6361 ttttaatttt tgtgctaata ctttagtttt tggtgcatcg atttcgaatt tctcaacaac
     6421 cactaaacgg tcttgacgaa ctaattcaga aagaatgctt ttgattgcac cacggtacat
     6481 tcttttgttc actttttggc tgtgatcttg tggtttcgca gcaaaagtta cactacccga
     6541 acgccaaatt ggtgatttaa tatcaccaga acgtgcacgg cctgtacctt tttgacgcca
     6601 aggtttttta cctgaaccag acacttcagc acgagttttt tgagcacgag atccttgacg
     6661 agcacctgct gcataagcaa caactacttg gtggattaat gcttcattga actcacgtcc
     6721 gaaggtagtt tcagaaacag ttagtgcgtt tgcacctaca acttgtaatt ccatctctat
     6781 ctcctagact tatgctttaa ctgccggttt tacgataaca tcaccattag ttgcaccagg
     6841 aacggcacct ttaactaata ataatttacg ctcaacatct acacgaacaa cttcaagtga
     6901 ttgaacggtt acacgctctg cacctaagtg tcctgccatt tttttacctt taaacacacg
     6961 acctggagtt tggttttgac caatagaacc aagtacacga tgtgataaag agttaccgtg
     7021 agttgcatct tgagtacgga agttccaacg tttaacacca ccttggaagc ctttaccttt
     7081 tgatgtacca gtaacatcga cttttttaac atcagtaaag atgtcaacat tgatttcttg
     7141 acctaaagtg aattcttcac cttcagtagt acgaaattcc cataaaccgc gtccagcttc
     7201 aacacctgct ttcacgaaat gacctgcttc tggtttagtt acacggctag cttttttaga
     7261 gccagtagta acttgaattg cagagtagcc atcgttttca agggttttaa cttgagtaac
     7321 acggttggct tcgatttcga taacggtaac tggaatagac acgccgtctt cagtgaaaac
     7381 gcgggtcata ccaactttac gaccgattaa accaatcatt gtaataacct cttaaaattc
     7441 ccaattaacc tagactaatc tgaacgtcaa cgccagcagc tagatctaaa cgcattaatg
     7501 catcaacagt tttttctgtt ggttcaacaa tatcaactaa acgtttgtga gtacggattt
     7561 catattggtc acgtgcatct ttattcacgt gtggagaaat caatacagtg aaacgttctt
     7621 tacgagttgg taaaggaatt ggaccacgaa cttgtgcacc agtacgttta gctgtttcta
     7681 cgatctccgc agtagattga tcaattaaac gatgatcaaa tgcttttaag cggatacgga
     7741 ttctttggtt ctgcattaga ccagagctcc aattaaaaaa tttagctaat aaaaaaacca
     7801 aactaccact taacaaatta gttaagggag ctcagttaaa cctatatagt tccaaaatta
     7861 ggaacattgt atacactaca atatctgtag tgccatttga taaatgatta catcaaatgt
     7921 ctttagatac ctaaagct
//
LOCUS       DANLZC010000114         7865 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.114, whole genome shotgun sequence.
ACCESSION   DANLZC010000114 DANLZC010000000
VERSION     DANLZC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.114"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            36..4232
                     /locus_tag="PXE44_001969"
     CDS             36..4232
                     /locus_tag="PXE44_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5589451.1"
                     /translation="MKTKTFTRSYLASFVTIVLSLPAVASVVRNDVDYQYFRDFAENK
                     GPFSVGSMNIDIKDNNGQLVGTMLHNLPMVDFSAMVRGGYSTLIAPQYLVSVAHNTGY
                     KNVQFGAAGYNPDSHHYTYKIVDRNDYEKVQGGLHPDYHTPRLNKLVTEVVPAAVTNA
                     GTSIKPYLNEERFPMFLRAGSGTQALRGKESNKTTGIAGAYEYLTGGTTLQLSKSSPD
                     HWLDYSSNLYQVSYGPLSTYALPGDSGSGSYAYDMNEKRWVLVGVLNFYNGMDNQFNR
                     SAIIRKDFHEKKFAEDIAGTINNTVQNAQFNWTAQGKSSSLSQSNNVQKLNVDLKDSS
                     IANQNTSLPQENHGKTINFNGKDATIVLKQDIDQGAGALNLNANLTIRPETDQTWQGA
                     GIIVGKDKKVNWQVKNPQGDRLSKLGEGTLYVNGRGQNLGDISVGDGIVILNQQADHQ
                     GRKQAFNTVGIVSGRPTVVLGSADQVNPDNIYFGFRGGRLDLNGNSIAFKRIQNSDKH
                     ARIVNHNRDHISTLIIQGQDPLTSNDLIWGKWASNSPADIYEYTNPYQNKRKDYFRLK
                     GNSRVYYPTNATSNDHWEFLSSNREQAIQKILDAKNLRQRYDTFNGFIGEDASNKTNG
                     ILNVVFDTKTEVNTEQDKLKNIYTMSGGFNLNGELTLKGGTLLLSGHPTPHAYDIKNK
                     HDVVRENDWQDSHFTAKNITVNKMAQLYIGRNVNEVNSHFTATDKAKLNLGFINRSTP
                     SCYDSEYTGTTHCEVQAVISDNIFANLATTAIKGNVKLQNHSQLNLGKANLTGSVQAD
                     QTTHITLANHSHWLNNGTSQIGHLTMEKGSILSLNDKFATTEIPVRFNKMIIQGNLKG
                     NGRINYTANLAKGESDHLQVDGIAEGNFVLAVRNSTTEANPKSSLNLLSLKNSNQEGN
                     KASISLENNYVDLGTYRYVLENRNHNYHLFNPLIPNSTSKEMNATSVSSIPKKESVTN
                     VPTLDKKETEQNLTQLQKDFSAHQLENQKAKQSMINAQSELRRLNSQLNVLQKYVNSR
                     RLGYYTQQAVLEQISIIQNKIKQTQTIFNDANATVKLTDQKLEEAKLALGSVNDLVLI
                     KASAPAMQATNQDTSMMNIIQADWISQYANTALSELSAQANSALQISNSLDRQLFKQS
                     DKFNVWSSVEHQKTEHKSDLYRPYKQQTNLTQLGIQMPIDNGLMFGVALSKNHANAEF
                     NEGVNGKSNLLMASLYGKWQSQQGTFISLDGSYGKAKNQLYLFGENHFTRRISSIGAN
                     IGHQFDLAGVQIQPTIGARYYHFSGQDYTLGGAKISSPNTHFMTYQAGLKASKTFSLD
                     DWKVEPSITTHYVDASNKRLAVNVNNNNINQRFGRYLKTEAGIRFEHNQWQFDLHAGF
                     INGNEIKKQHFAGVKLGYSW"
     gene            complement(4287..5381)
                     /gene="mobA"
                     /locus_tag="PXE44_001970"
     CDS             complement(4287..5381)
                     /gene="mobA"
                     /locus_tag="PXE44_001970"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL5589452.1"
                     /translation="MSKTIEERISAVILAGGLARRMNGVEKGLQLFEGKPLISHILAR
                     LSPQVSDIWLNVNRSIEQYQQLYPAFSYYQDSLPDFQGPLSGMLAGFEQIESDYLLFV
                     PCDTPFMPELLLQKLKTALRINNVQIAYAHDGERPHPTFALIHRSVQEDLKAYLGSNQ
                     QQLLAFFQSQKSVAVDFSEQKSAFTNVNTLEDLSRVSQFPVKNLAITGYSGTGKTTLL
                     EKLIPKLTACGIRVGLIKHSHHNVDVDKKGKDSYRLREAGANPTMIVCDERWALMTET
                     KQAVEFSQLIAKFNPQEVDLVFVEGFKHETLPKIQLHRKGIEKPLPELDQWTIATATD
                     YPLERENWLDINNIGGIADFIKNWLENKAS"
     gene            5468..5737
                     /locus_tag="PXE44_001971"
     CDS             5468..5737
                     /locus_tag="PXE44_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1040 family protein"
                     /protein_id="HDL5589453.1"
                     /translation="MKCERLEELLELLGEHWRKNPDLHLIDILQQLSVEVGEPDNFKA
                     LSDEVLIYQLKMRNAGKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            5801..6439
                     /locus_tag="PXE44_001972"
     CDS             5801..6439
                     /locus_tag="PXE44_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL5589454.1"
                     /translation="MKKLALKTAFVALSALFTFNSTAIAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELSYKIPSQIKEKLPSDSKLVQYHVNFLGRQSEDLTRAWAL
                     AMALGAEDKVKTALFEGAQKDAFKSMDDIRSVFLANGITAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFADSSSTNDFIKRYVDAVVFLSKK"
     gene            6523..7299
                     /locus_tag="PXE44_001973"
     CDS             6523..7299
                     /locus_tag="PXE44_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL5589455.1"
                     /translation="MTKLSILNLVPIREGQTAKQAVDSMVKLAKHAESIGIERYWIAE
                     HHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLPNHSPYIVAEQYGTLETLYPNRV
                     ELGLGRAPGTDMRTATALRRNAQSLPFPDEISELKGYFEGTNSVSAYPAQGLNVPFYI
                     LGSSPESAYLAAERGLPYAFASHFAPRFMDDAVRIYRTHFKPSEVLDKPYVILGVNAI
                     AAETDQEAEQLATTQTQFFLNVVTNAQNYLQPPVESDDKV"
     gene            7309..7563
                     /locus_tag="PXE44_001974"
     CDS             7309..7563
                     /locus_tag="PXE44_001974"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase"
                     /protein_id="HDL5589456.1"
                     /translation="MKAEIVPHFGPVSFQQNALYNQEKPVVRQMTACSLIGSRESIEQ
                     QLKSLKARIEIDEIMAVSYIFDEQKQHQSYTWLKEIADKV"
     gene            7565..7858
                     /locus_tag="PXE44_001975"
     CDS             7565..7858
                     /locus_tag="PXE44_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5589457.1"
                     /translation="MSKTMQQKAADAHNMCKLKGDSMLDNSDRQIAFEAVYDSEEIAV
                     QAVEFFTQKAKSVETEPCDIQSEITPIIDGFLMKMWITFSCQAEVILFQMAVR"
BASE COUNT         2627 a         1556 c         1479 g         2203 t
ORIGIN      
        1 attttttatt acttataagg ataaacttct catttatgaa gaccaaaaca tttactcgtt
       61 cttatcttgc ttcttttgta acaatcgtat taagtttacc tgctgtagca tctgttgtac
      121 gtaatgatgt ggactatcaa tacttccgcg attttgccga aaataaagga ccattttcag
      181 ttggttcaat gaatattgat attaaagaca acaatggaca acttgtaggc acgatgcttc
      241 ataatttacc aatggttgat tttagtgcta tggtaagagg tggatattct actttaattg
      301 caccacaata tttagttagt gttgcacata atactggata taaaaatgtt caatttggtg
      361 ctgcaggtta taaccctgat tcacatcact atacttataa aattgttgac cgcaatgatt
      421 atgaaaaggt tcaaggaggg ttgcacccag actatcatac tcctcgatta aataaattag
      481 taacagaagt tgtgcctgcc gcagtcacca atgcaggtac atctattaaa ccctacttaa
      541 atgaagaacg cttccctatg tttcttcgtg ctggttcagg gacacaagcg ctaagaggaa
      601 aagaaagtaa taaaacaact ggaatcgctg gtgcttatga atatcttact ggcggtacca
      661 cattacaatt atctaaaagc tcccctgatc actggttaga ttattcaagt aacctttatc
      721 aagtaagcta tggaccactt tcaacctatg cactacctgg tgatagtggt tcaggttctt
      781 acgcctatga tatgaacgaa aaacgatggg tattagttgg tgtgctcaat ttctataatg
      841 gtatggataa tcaattcaac cgctctgcga ttatccgtaa agatttccac gagaaaaaat
      901 ttgccgaaga tattgcagga acaatcaata ataccgtaca aaatgcacaa ttcaattgga
      961 ctgctcaagg taaatccagc tctcttagtc aatcaaataa tgtgcaaaaa ctcaacgttg
     1021 atctaaaaga tagtagcatt gcaaaccaaa acacttctct gccacaagaa aatcacggta
     1081 aaaccattaa ttttaatggt aaagatgcaa ctattgtact aaaacaggat attgaccaag
     1141 gtgcaggtgc attaaatctg aacgctaatc tcactattcg tcctgaaaca gaccaaactt
     1201 ggcaaggtgc aggtattatc gtcggtaaag ataaaaaagt gaattggcaa gtaaaaaatc
     1261 cacaaggcga tcgtttatct aaactcgggg aaggaacact ctatgtaaat ggacgtggac
     1321 agaatcttgg cgatatcagt gtaggtgatg gtattgtaat acttaaccag caagccgatc
     1381 accaaggaag aaaacaggcc tttaatacag taggaatcgt aagtggtcgc cccactgttg
     1441 tgctaggtag tgcagatcaa gttaatcccg ataatattta ctttggattt cgcggaggtc
     1501 gtttagacct aaacggtaac agcatcgcct ttaaacgtat tcaaaacagc gataaacatg
     1561 ctcgtattgt aaaccacaat cgcgatcaca tttctacctt aataatacaa ggccaagatc
     1621 ctctcactag taatgatctt atatggggaa aatgggcaag taatagccca gcagacattt
     1681 acgaatatac caatccttat caaaataaac gcaaagatta cttccgtctg aaaggtaatt
     1741 cgagagtata ttatccaacg aatgctacaa gtaacgatca ctgggaattt ctttccagta
     1801 accgcgagca agcaatacag aaaatcctag atgccaaaaa cttaagacag cgctatgaca
     1861 cgtttaatgg ttttataggg gaagatgctt ccaataaaac taatgggata ttaaatgtcg
     1921 tgtttgatac aaaaacagaa gtaaatacag aacaagataa attaaagaat atctacacaa
     1981 tgtcgggagg atttaacctt aatggtgaac tcacccttaa aggtggtaca ttgttgcttt
     2041 ctggtcaccc aacgccacac gcttatgata ttaagaataa gcatgatgtt gtgcgtgaaa
     2101 acgattggca agacagccat tttactgcta aaaatatcac ggtaaataaa atggcacaac
     2161 tctatatcgg gagaaatgtc aatgaagtaa atagtcactt tactgcgact gataaagcca
     2221 aactcaattt aggatttatt aatcgttcaa cgccaagttg ctatgattct gaatacacag
     2281 gcactacaca ttgtgaagtg caagcggtca tttccgataa tatttttgca aatctagcaa
     2341 caaccgccat taaaggtaat gttaaattac aaaaccatag ccaattaaat ttaggcaaag
     2401 caaacctcac tggttctgta caagctgatc aaacaactca tatcacttta gcaaatcaca
     2461 gtcactggtt aaacaatggt acgagccaga ttgggcatct tacaatggaa aaagggtcga
     2521 tccttagcct aaacgataaa tttgctacca cggaaatccc agtccgattc aacaagatga
     2581 tcatccaagg taatctaaaa ggtaatggac gaattaacta taccgcaaat ttagccaagg
     2641 gcgaatctga tcatctccaa gttgacggta ttgctgaagg aaattttgtc cttgccgtta
     2701 gaaatagcac aactgaagca aatccaaaaa gctcattaaa cctactaagc ttaaaaaata
     2761 gcaaccaaga aggcaataaa gcttctattt ctctagaaaa taattatgtt gatctaggta
     2821 cttatcgtta tgtattagaa aatcgtaatc acaattacca tttatttaat ccattaatac
     2881 caaattcaac ctctaaagag atgaatgcta catctgtatc ctctattcca aaaaaggaat
     2941 ctgttactaa tgttcctact ttagataaga aagaaactga acaaaatctt actcaactac
     3001 aaaaagattt ttcagcacac caattagaaa atcaaaaagc aaaacaatct atgataaatg
     3061 ctcaatctga gctaagacga ctcaattcac aactgaatgt attgcaaaaa tatgtgaatt
     3121 ctcgtcgctt aggttactat actcagcagg cagttttaga acaaattagc attattcaaa
     3181 ataaaattaa acaaacacaa acaatattta atgacgctaa tgcaactgta aaactcacag
     3241 atcaaaagct agaagaagcc aaattagctc taggctctgt aaacgatctt gtattaataa
     3301 aagcctctgc tccagcaatg caagcaacta atcaagatac gagtatgatg aatattattc
     3361 aagcagattg gataagccaa tacgctaaca cagcactttc tgaactctcg gcacaggcta
     3421 attctgctct gcaaatcagt aatagcttag atcgccaact cttcaaacaa agcgataaat
     3481 tcaacgtatg gagcagcgtc gaacatcaga aaaccgagca taaatcagat ttataccgcc
     3541 cgtataaaca acaaaccaac ctgacccaac tgggcataca aatgccgata gataacggtt
     3601 taatgtttgg agttgcatta tctaaaaacc acgctaacgc ggaatttaac gagggtgtaa
     3661 acggtaaatc gaatctacta atggcaagcc tatatggtaa gtggcaatct caacaaggca
     3721 cttttatcag ccttgatggc agctacggta aagcaaaaaa ccaactctac ctatttggtg
     3781 aaaaccactt tacccgccga atttcctcta ttggtgctaa cattggacat caatttgacc
     3841 tcgcaggagt tcaaattcag ccaacaatag gagcaagata ctaccatttc agcggccaag
     3901 actatacact aggaggagcg aaaatcagct caccaaatac ccactttatg acatatcaag
     3961 cgggtctaaa agctagtaaa accttttcat tggatgactg gaaagttgaa ccaagcatta
     4021 caacccacta tgtggatgca agtaacaaac gcttagcagt aaacgtcaat aataacaata
     4081 tcaaccaacg tttcggtcgc tatctgaaaa ccgaagctgg tatccgattt gaacataacc
     4141 aatggcaatt cgacttacac gctggtttca ttaacggcaa cgaaatcaaa aaacaacatt
     4201 ttgctggtgt taagctagga tatagctggt aagaaaaagc ccctagaact atttctaggg
     4261 gctttttcta ctcttaaaaa gagtatctaa cttgccttat tttctaacca attcttaata
     4321 aaatctgcta ttcccccaat attattaata tccaaccaat tttctctctc aagcgggtaa
     4381 tccgttgcgg tggcaatcgt ccattggtct agctctggca atggtttttc aatccctttg
     4441 cggtggagct gaatttttgg caaggtttcg tgtttaaagc cttctacgaa aacgagatca
     4501 acctcttgcg ggttgaattt tgcaattaat tgagaaaatt cgaccgcttg tttggtttct
     4561 gtcattaatg cccaacgttc atcgcacaca atcattgtcg ggtttgctcc tgcttcacgt
     4621 aggcggtagc tgtctttgcc ttttttatct acatcaacgt tatggtggga atgtttaatc
     4681 agcccaaccc gaataccgca agcggtcaat tttgggatta atttttccaa taaggtggtt
     4741 ttacctgtgc cgctgtagcc tgtaattgca aggtttttta cagggaattg gcttactcgg
     4801 cttaaatctt ccaatgtatt cacgttggtg aatgccgatt tttgttcgga aaaatcaact
     4861 gccacgcttt tttgtgattg gaagaacgca agcaattgct gttggtttga gcctaaatag
     4921 gctttcaaat cttcttgtac cgaacgatga atcaacgcaa aagtgggatg tggacgctca
     4981 ccatcatggg catatgcaat ttgtacgtta tttatccgca aagcagtttt taatttttgc
     5041 aataacagct caggcataaa gggcgtatcg caaggcacaa aaagcaaata gtcactttca
     5101 atttgctcaa atcctgccag cattccactg agtggacctt gaaaatcagg caagctatct
     5161 tgatagtaag aaaaagcagg ataaagctgc tgatattgct caatagaacg attgacatta
     5221 agccaaatgt cactaacttg tggtgaaagt cgggctaaaa tgtgtgaaat aagcggtttt
     5281 ccttcaaaaa gttgtaaccc tttttctacc ccattcattc gacgagctaa accgcccgct
     5341 aaaatcacgg ctgaaatgcg ttcttctatt gttttactca cagctttatc ccttaaaatt
     5401 ttgcaaaaat aagagtaaaa gtatattatt tcgtttaatt tccaacaagt tttttaaggg
     5461 gtaaaagatg aaatgcgaac gtttagaaga gttattagag ttacttggcg aacattggcg
     5521 taaaaatcct gacttacacc tcattgatat tttgcagcag ctttcagttg aagtgggcga
     5581 gcctgataat ttcaaagcgt taagcgatga agtgttaatc tatcagctta aaatgcgaaa
     5641 tgcaggcaaa tttgagccta ttcccggcat aaaaaaagat tatgaagatg attttaaaac
     5701 ggctttattg cgagctcgtg gaattttaaa cgattaagat tgtcttatcc gttagctagt
     5761 ttagcattta ttatttatca acatttttga aggaatcatt atgaaaaaat tggcattaaa
     5821 aacggcattt gttgcacttt ctgccctttt tacatttaac tcaacggcaa tcgctgctga
     5881 tccaaccgca ggcaaagaat atatcgaagt gcgtaaagca ccttctgcac aaaaagaagt
     5941 ggtggaattt ttctcattct actgcccaca ctgttatgat tttgaattaa gctacaaaat
     6001 cccaagccaa atcaaagaaa aattaccaag cgatagcaaa ttagtgcaat atcacgttaa
     6061 tttcttaggt cgccaatctg aagatttaac tcgtgcttgg gcattagcaa tggcattagg
     6121 tgcggaagat aaagtaaaaa ccgcattatt tgaaggtgct caaaaagacg cattcaaatc
     6181 aatggacgat attcgctctg tattcttagc aaacggcatt acagctgaac agtttgatag
     6241 cggtattaat agctttgcgg taaatggctt agtgaataaa caagttcaat tagcggaaga
     6301 cttccaaatt cgtggtgtgc ctgcattctt cgtaaatggt caataccaac tcaacttaga
     6361 aggctttgca gattcttcat caacgaatga tttcatcaaa cgttatgttg atgcggttgt
     6421 gttcttaagc aagaaataac cccttaaaat agttagggtt tttacaatgc tgagtaggta
     6481 aatcctattc agcatttttt atatgcaaac tcggagaaca aaatgacaaa gttatctatc
     6541 ctaaaccttg tacctattcg tgaagggcaa actgccaaac aagcggtcga ttctatggtg
     6601 aaattagcaa aacacgcgga aagtatcggc attgaacgct attggattgc agagcaccac
     6661 aatatgaaaa accttgccag ctcggcaaca cagctcctca ttcagcatgc cctttctcac
     6721 actgaaaaat taagagtggg atcaggtggc gtgatgttgc ctaaccatag tccttatatt
     6781 gtggcagagc aatacggtac gcttgaaaca ctctacccta accgtgtaga acttgggctt
     6841 gggcgagcac caggcaccga tatgcgaacc gctaccgctt tacgccgtaa tgcacaaagt
     6901 ctgccatttc ctgatgaaat cagcgaacta aaaggttatt ttgaaggcac aaattctgtg
     6961 tctgcctacc ctgcacaggg gttgaatgtg ccgttttata ttttaggctc aagccctgaa
     7021 agtgcctatt tagcagcaga acgtggcttg ccttatgcct ttgcttcaca tttcgctcct
     7081 cgttttatgg acgatgcggt gcgaatttat cgcacacact ttaagccgtc tgaagtgtta
     7141 gataaaccct atgtgatttt aggtgtgaat gcgattgcgg cagaaaccga ccaagaggca
     7201 gaacagcttg ctaccactca gactcagttt ttcttaaatg ttgttaccaa tgcccaaaac
     7261 tatttacaac cgcccgtcga atccgatgat aaagtgtgaa aaaattttgt gaaagcggaa
     7321 atcgtaccgc atttcgggcc tgtttcgttc caacaaaatg cgttatacaa ccaagagaaa
     7381 cccgttgttc gccaaatgac tgcttgttca cttatcggta gccgagagag cattgaacaa
     7441 cagctcaagt cattaaaagc acgcattgaa attgatgaaa ttatggcggt aagctacatt
     7501 ttcgatgagc aaaaacagca tcaatcctat acttggttaa aagagattgc agataaggtg
     7561 tgatatgagc aaaacaatgc aacaaaaagc cgctgatgca cataatatgt gtaagctaaa
     7621 aggcgactct atgttagata actcagaccg ccaaattgcc tttgaagcag tgtatgatag
     7681 tgaagaaata gctgtacaag cggtcgaatt tttcacacaa aaagcaaaat cggtagaaac
     7741 cgagccttgc gacattcaaa gtgaaatcac acccattata gatggctttc tgatgaaaat
     7801 gtggataact ttcagttgcc aagcagaagt aatcttattt caaatggcgg tgagatagat
     7861 ataaa
//
LOCUS       DANLZC010000115         7733 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.115, whole genome shotgun sequence.
ACCESSION   DANLZC010000115 DANLZC010000000
VERSION     DANLZC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.115"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..2538
                     /gene="topA"
                     /locus_tag="PXE44_001976"
     CDS             <1..2538
                     /gene="topA"
                     /locus_tag="PXE44_001976"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL5589458.1"
                     /translation="NYVVKSSVGHIRDLPKSGSDEKTEKAKPISTKGLTAAEKAKIKA
                     EKERVALVKRMGIDPYNGWKANYQILPDKEKVVSELKSLAKKADHIYLATDLDREGEA
                     IAWHLREIIGGDDSRFSRVVFNEITKDAIKKAFDDPQPLNIDRVNSQQTRRFLDRVVG
                     FMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREIKAFQSEEFWTILANTQINRKKLP
                     LELTTYKGKKFSASNEAEANVAVRALEQSDFIVSNIEAKETSSRAAAPFITSSLQRAA
                     STRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLSREALAMARGYIQDNFGANYLPEK
                     ANVYRSKDNAQEAHEAIRPSDVNVKMADLKGMDKDAERLYDLIWRRFLACQMLPARYD
                     STAVTVSAGDYELKANGRVLRFDGWTKVLPVQGKNPEDQELPAVELHQKLTLDEIVPS
                     QHFTKPPARFNEASLVAELEERKIGHPSTYASIISTIQERGYVRTENKRFYVEKIGEV
                     VTDRLNESFKDLMNYDFTANMEEVLDQIAEGSKNWKEELNTFFKHFSEELSQAELDEL
                     EGGMKPNSLVPTDIDCPTCGRKMAIRTASTGVFLGCTGYALPPKERCKTTMNLIPEAE
                     FFNVLDSDSETKALMARKRCPKCDSAMDSYVIDAERKLHVCGNNPNCDGHIIEQGSFK
                     IKGYDGPVVECDKCGSDMHLKLGRFGKYMGCTSCDNTRKILKNGEVAPPKEDPIAFPE
                     LKCEKADAYFVLRDGASGVFMSAHNFPKVRESRAPKVVELAQYRERLPEKLQYLADAP
                     QQDPEGNEAIIRFSRKEKRQYVTSEKNGKATKWIVDYLDGKWVERKK"
     gene            complement(2588..3526)
                     /gene="yvcK"
                     /locus_tag="PXE44_001977"
     CDS             complement(2588..3526)
                     /gene="yvcK"
                     /locus_tag="PXE44_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDL5589459.1"
                     /translation="MENTDIIPFKPTRHHYLNQLNAVVALGGGHGLGRLLSALSFLKE
                     RLTGIVATTDNGGSTGRIRSQQGVIAWGDLRNCLNQIIVKPTIASRLFEYRFAGTGEL
                     AGHNLGNLILTALENMQIRPLEGINLVRELLHVRSQLIPMSEIPVHLIAQLHSGESVF
                     GEVQIDSLAETPQHLALDTMVVATPEAISAIQSAELILLGPGSFFTSIMPNLLVAGIA
                     NSIAQSKARVVFIDNIGQEHGPAGQLSLNQRIEWMENCIGQARINAVITTDDAGSELP
                     EHIQILRKNLSANDVYYRHDRNKLSKAIDELLAQNI"
     gene            complement(3540..4616)
                     /locus_tag="PXE44_001978"
     CDS             complement(3540..4616)
                     /locus_tag="PXE44_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL5589460.1"
                     /translation="MFQMLQDWYRKKFTDPQIVVLFSILLIGFGIIYFFSDLLMPLLV
                     ALVFAYLLEWPIRFLSSKLKLPRTLSVILVLGGFIALLSFLGVVLLPSLWNQAVTFIQ
                     DLPSMFNLLNAWLQALPEHYPELVDYATLDSIVNTAKSNILSMGESLLTLSINSIISL
                     VGLGIYTFLVPLMVFFLLKDKIVLMRSFSKVLPQNRRLATRVWFEMQQQIANYIRGKF
                     LEILIVGVVTYIIFLFFDLRYPLLLSVAVGISVLVPYIGAVLVSIPVMLIALFQFGLS
                     PDFYYLMLAFIISQLLDGNLLVPFLFSEAVNLHPLTIIIAVLIFGGLWGFWGVFFAIP
                     LATLVKAVLQALPSAQSDAIILEK"
     gene            complement(4647..5402)
                     /locus_tag="PXE44_001979"
     CDS             complement(4647..5402)
                     /locus_tag="PXE44_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5589461.1"
                     /translation="MKKEEVGHHFLARLGKTRLRPGGKLATDWLIANGDFDKNKKVLE
                     VACNMGTTAIQLAQQFECQIVGIDLDEDALEKARKNIKENHVEHLIQVQRANATKLPF
                     DDESFDIVINEAMLTMLPLEAKEKAIREYFRVLKPNGFLLTHDIVIQDKDSDTLLAEL
                     RDAINITVSPLAKADWKSLFQRCGFRNVDTFSGEMSLLSPKGLIHDEGVLGAMKIIGN
                     ALKPENRETFNKMYKLFNDPDKKLGFIAVCSQK"
     gene            5554..5763
                     /locus_tag="PXE44_001980"
     CDS             5554..5763
                     /locus_tag="PXE44_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HDL5589462.1"
                     /translation="MSKQKNDYLHQRGQIKESAVKALVTDPLFRQRVERNRKGKGSYR
                     RQEKHKKTIYGQKETPFKNIRLESF"
     gene            complement(5812..6534)
                     /gene="bioD"
                     /locus_tag="PXE44_001981"
     CDS             complement(5812..6534)
                     /gene="bioD"
                     /locus_tag="PXE44_001981"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDL5589463.1"
                     /translation="MPSLFITGTDTNVGKTVVTRAIIQTLTKHSFPVIGYKPIACGGD
                     DSLPTEPTQYDYATEDNSDVLTILDSCPTNVSYRDINSYTFIHSSTPVFAALDAVHHI
                     QLEKLNTDLKRLENAYPNVVVEGTYGWLTPINKDYYFADWVMQNNMPVVLVVGIKEGC
                     VNHALLTANEILQRGVKLVSWVANRVNPGLRHYHELIELLTQKINAPLLGQIPYMGHP
                     EHKELSSYIQNPEPLLAYFSKP"
     gene            complement(6582..7722)
                     /locus_tag="PXE44_001982"
                     /pseudo
     CDS             complement(6582..7722)
                     /locus_tag="PXE44_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705544.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
BASE COUNT         2473 a         1403 c         1594 g         2263 t
ORIGIN      
        1 aattatgtgg tgaaatcgag tgtggggcat attcgtgatt taccgaaaag tggtagtgat
       61 gaaaaaacgg aaaaagcgaa accgatttca accaaagggc tgaccgctgc cgaaaaagct
      121 aaaatcaaag cagaaaaaga gcgtgttgca ttggttaaac gtatgggtat cgacccctat
      181 aatggctgga aagctaacta tcagattttg ccggataaag agaaagtggt ttccgagcta
      241 aaatcgttgg caaagaaagc agatcatatc tatctggcaa ccgacttgga tagagaaggg
      301 gaggcgattg cgtggcattt gcgtgaaatc attggtggtg atgacagccg ttttagccga
      361 gtggtattta atgaaatcac caaagatgcg atcaaaaagg cttttgacga tccgcaaccg
      421 ctcaatattg accgtgtaaa ttcccagcaa acccgccgct ttttagaccg tgtagtgggc
      481 tttatggtgt cgccattatt atggaaaaaa gtagctcgtg gtttatcggc agggcgtgtg
      541 caatcggttg ccgtaaaatt actggttgag cgtgagcgtg aaatcaaggc gttccaaagc
      601 gaggagtttt ggactattct tgccaatacg caaattaatc gtaaaaaatt accgcttgaa
      661 ttaactactt ataaaggtaa aaaattctcg gcaagcaatg aggctgaagc aaatgttgca
      721 gtgcgtgctt tggagcagag cgattttatt gtttctaata ttgaagccaa agaaaccagc
      781 tctcgagctg ctgcaccatt tattacctca tcgttacaac gtgccgctag cactcgttta
      841 ggttttgggg ttaaaaagac gatgatgtta gcccaacggt tatatgaagc gggttacatc
      901 acctatatgc gtactgattc aaccaacctt agccgtgaag cattagcaat ggctcgaggt
      961 tatattcagg ataatttcgg ggcgaattat ttgccggaaa aagcaaatgt gtatcgcagt
     1021 aaagataatg cccaagaagc tcacgaagcc attcgcccgt cggatgtgaa tgtcaaaatg
     1081 gcggatttaa aaggaatgga taaagatgcc gagcgtttat acgatttaat ttggcgtcgt
     1141 ttcttggcgt gtcaaatgtt gcctgctcgt tatgattcta ccgcagtcac agtgtctgcc
     1201 ggagactatg aactcaaagc aaacgggcgt gtactgcgtt ttgacggctg gacgaaagta
     1261 ctgccggtgc aaggcaaaaa tccggaagat caggaattac cggcggttga actgcatcaa
     1321 aaattaacgc ttgatgaaat tgtgccaagt cagcatttta ctaaaccgcc tgcacgtttt
     1381 aatgaggcgt ctttggttgc tgagttagaa gagcgaaaaa ttggccaccc ttcaacctat
     1441 gcttcaatta tttcgacgat tcaagaacgt ggctatgttc gcaccgaaaa caagcgtttt
     1501 tatgtggaaa aaatcggtga agtagtgacg gatcgcctca acgaatcatt caaagattta
     1561 atgaattatg atttcactgc caatatggaa gaggtgctcg accaaattgc agaggggtct
     1621 aaaaattgga aagaagagtt gaatactttc tttaaacatt tctctgaaga gttgagccaa
     1681 gcagaattag atgaacttga aggcggaatg aaaccaaaca gccttgtgcc aaccgatatt
     1741 gattgcccga cttgtggacg aaaaatggca attcgcaccg cttccacagg ggtattttta
     1801 ggttgcaccg gctatgcttt accgccaaaa gagcgttgca aaacgacaat gaatttaatt
     1861 ccagaggcgg aatttttcaa tgtgttagat tcggattctg aaaccaaagc cctaatggct
     1921 cgcaagcgtt gcccgaaatg cgattcggca atggatagtt atgtgattga tgctgaacgt
     1981 aaattacacg tttgtggtaa taatccaaat tgtgatggtc atattattga gcaaggttcc
     2041 ttcaaaatta aaggctatga cggacctgta gtggaatgcg ataaatgcgg ttcagatatg
     2101 cacttaaaac tgggtcgttt cggcaaatat atgggctgca caagctgcga taacacccgt
     2161 aaaattctga aaaacgggga agttgctccg ccaaaagaag atcctattgc ttttcctgaa
     2221 ttaaaatgtg aaaaagccga tgcctatttt gtattaagag acggagcaag tggggtgttt
     2281 atgtcagctc ataacttccc gaaagtgcgt gaaagccgtg caccaaaagt ggtagagtta
     2341 gctcaatatc gtgaacgttt gccggagaaa ttgcagtatc ttgccgatgc tccgcagcaa
     2401 gatccggaag gaaacgaggc aattatccgt tttagtcgta aagagaaacg ccaatatgtg
     2461 acttctgaga aaaacggcaa agccaccaaa tggattgttg attatctaga cggtaaatgg
     2521 gttgagcgta agaaataaag cttaaaagac aagcggtaaa attttctaaa aattttaccg
     2581 cttgctttta gatgttctga gcgagtagtt catcaattgc tttacttaat ttattgcgat
     2641 cgtgtcggta ataaacgtca ttcgcagata agttttttcg caagatttga atatgttcgg
     2701 gaagttcaga accggcatca tcagtcgtaa ttaccgcatt aattctagcc tgtccgatac
     2761 agttctccat ccattcaatt cgctgattaa gtgatagttg cccagctggg ccatgttctt
     2821 gtccgatatt gtcaataaat actactcggg ctttgctttg agcaatagag tttgctatgc
     2881 ctgccaccag caaattcggc ataatactgg taaaaaagct acctgggcct agcagtatta
     2941 actcagcact ttgaatagct gaaatggctt ctggtgtagc aacaaccata gtatctaacg
     3001 ctaaatgttg tggtgtttct gctaaagaat caatttgcac ttcaccaaat actgattcgc
     3061 ctgaatgcaa ctgtgcaatt aaatgcaccg gtatttcaga cattgggata agttgcgatc
     3121 ttacgtgcaa taattctcgc accaaattaa tcccttctag cggtcggatt tgcatatttt
     3181 caagggcggt gagaattaaa ttgcctagat tatgtccagc gagttcgcct gtgccagcaa
     3241 aacgatactc aaacaaccga gaagctatcg ttggtttaac gataatttga tttaaacagt
     3301 tgcgtaaatc tccccaagca atcacccctt gttgtgaacg tattctaccg gttgagcctc
     3361 cattatcggt agtggcaaca atgccggtaa gccgttcctt taaaaaagag agcgcggaga
     3421 gcaatcgacc taagccgtgg cccccaccta gtgcaacaac tgcattgagt tggtttaaat
     3481 agtgatgtcg ggtaggttta aaaggaataa tatctgtatt ttccatcttg ggatttcgtt
     3541 tatttttcta aaataatggc atctgactgg gctgaaggca atgcttgtaa tacagcttta
     3601 accaaagtcg ctaaaggaat cgcaaaaaac actccccaaa agccccataa accaccaaag
     3661 atgagtacgg caataataat tgtcagaggg tgtagattta ctgcttcaga aaataaaaag
     3721 ggtacgagta aatttccgtc tagcaactga ctaataataa atgcgagcat taaatagtag
     3781 aaatccggcg ataacccgaa ctggaataga gcaattaaca ttaccggaat gctgactaaa
     3841 accgcaccga tgtagggaac taacactgaa atccctaccg ctacagaaag tagtaacgga
     3901 tagcgtaaat caaaaaagag gaaaataata taagtgacaa ccccaacaat tagaatttct
     3961 aaaaatttac ctcgaatata gttggcaatt tgttgttgca tttcaaacca tactcgtgtt
     4021 gcaagtcgac ggttttgcgg taatacttta ctaaaagaac gcattaaaac gattttatct
     4081 ttcagtaaga aaaagaccat gagtgggact aagaaggtgt aaatgcctaa gccaactaag
     4141 ctgatgatag agttaatcga gagtgttaga agtgattctc ccatactcaa aatattactt
     4201 ttagcggtat tcacaatgga atctagggtc gcataatcta ctaattcagg gtaatgctcc
     4261 ggtagggctt gtaaccaagc attgagcaga ttaaacatcg aaggtaaatc ttgaataaag
     4321 gtaactgctt gattccaaag gctgggtagt agtactacgc ctaaaaaaga taataaagcg
     4381 atgaaacctc ctagtactag aatgacactc aaagttcgag gtaatttgag tttgctcgac
     4441 aaaaaacgga ttggccattc caataaatag gcaaacacaa gtgcaaccag aaggggcata
     4501 agcagatcgc taaaaaaata gataataccg aaaccgatca atagaatact aaacagcaca
     4561 actatttgcg ggtcggtaaa tttttttcga taccaatctt gcaacatttg aaacatatgg
     4621 attgccttta aattatgcga tgaaaattat ttctggctac agactgcaat gaaccctagc
     4681 tttttgtccg ggtcattaaa tagtttgtac attttattaa aggtttcgcg attttctggt
     4741 tttaatgcat tgccgataat tttcatggcg cctaaaacac cttcatcatg aatcagacct
     4801 ttaggggaaa gtagagacat ttctccggaa aaggtatcaa cattacggaa accacaacgt
     4861 tggaataatg atttccagtc tgctttagca agtggagaga cagtgatatt gattgcatca
     4921 cgcaactcgg ctaataaagt atcggagtct ttatcttgga taacaatatc gtgcgttaat
     4981 aaaaagccat ttggttttaa tacacgaaaa tattcacgta ttgccttttc tttcgcttct
     5041 agaggaagca ttgtcagcat ggcttcatta attacaatat caaaactttc gtcgtcaaat
     5101 ggcagttttg ttgcgttggc gcgctgtact tgaattaaat gctcaacatg gttttctttg
     5161 atatttttac gtgctttttc aagcgcatct tcgtctaaat caataccaac aatttggcat
     5221 tcaaattgtt gagctaattg gattgcagta gtccccatgt tacaggcaac ttcgagcact
     5281 tttttatttt tatcaaaatc cccgtttgca attaaccaat cagttgctaa tttccccccg
     5341 gggcgtaaac gagttttgcc taaacgagct aaaaaatgat gaccaacttc ttcttttttc
     5401 ataaaaacct ctggatttgc aaaatacgtt aaatatatga ccgcttgtat tgtgatctat
     5461 gttcgttatt atactcaatt ttttcatttt cttatataca acatcaacag attttgccag
     5521 aatacaacct cattttgata tgaggaaaat agaatgagta agcaaaagaa tgattacctc
     5581 caccagcgag gtcaaattaa agaaagtgca gtaaaagcat tagtgactga tccacttttt
     5641 agacaacgtg tagaaagaaa tcgtaaaggg aaagggagct atcgcagaca agaaaaacac
     5701 aaaaaaacta tctatgggca aaaagaaacc ccatttaaaa atattcggtt agagtctttt
     5761 taaatggggt tttatttgta gcttattgta gttttataag cctgataaac actatggttt
     5821 actgaaatag gccaacaacg gttctggatt ttgaatataa gaagaaagtt ctttatgctc
     5881 tgggtgaccc atatagggaa tttgcccaag taaaggcgca ttaatttttt gtgtcagtag
     5941 ttcaattaat tcgtgataat gccgtagccc tggatttact ctgtttgcga cccaacttac
     6001 taattttaca ccacgctgca aaatttcatt cgcagtaagt agtgcatggt taacacaacc
     6061 ttccttaatt cccaccacta aaacaaccgg catattgttt tgcattaccc aatcagcaaa
     6121 atagtaatcc ttgttaatag gagttagcca gccataggtt ccttctacga caacatttgg
     6181 ataggcattt tctagtcgtt ttaaatctgt gtttaatttt tccagttgaa tatgatgaac
     6241 tgcatctaaa gctgcaaata caggggtact ggaatgaata aaagtatagc tattaatatc
     6301 acgatagcta acattagttg gacagctatc tagtatggtt aatacgtctg aattatcttc
     6361 agtagcataa tcatattgag taggttctgt gggtaatgaa tcatcaccgc cacaagcaat
     6421 gggtttatag ccaattacag gaaaactatg ttttgtaagt gtctgtatga tggctcgggt
     6481 aaccacagtt ttccctacgt tggtgtctgt tccggtaata aataatgagg gcataaatgg
     6541 ctcctttcga aaatatggct ggtagtttaa ccaaaaatag atcaatttat tctgtctttg
     6601 atcaaatttc cgtcataaat tccttgtttt attgctgaac aggcaattaa tggactattc
     6661 caatcgtatt ggctggtaac taaatcgatt tttaggtttg tttgtaacaa atttttatgc
     6721 aatttttctg aaatttgcgt aaataacggc tcttttattt tgatcaatgg cgagtttagc
     6781 ataatttttt cactagaaaa tatattaact aaattcatta atgcataagc taaattatcg
     6841 gtaatatgcg ataaaatagc taatgcaagc ggttgatttt catcaatttt ttgacaaagc
     6901 cagctgattt tctgttcctg actttttaaa ttattaggca aataacgatc aatgagtctc
     6961 acaatagctg ggaacgtcac ctgattgact aatttatagc gatgaatttc atctagttca
     7021 gggaatattt catcactgag atggctaaat ctaggcataa gcatattatc aacattcatc
     7081 tttgattgtt tagacagtaa actaccacta agcaatacac ttagattcac agtatcatct
     7141 aattgtagaa aaatagcatt atcttggtta attaatgagc caaccgtaga ttctgcaagc
     7201 aaccacagct gaaaatgttc acttaataaa ataggacaat gaaacagcgg cgagagttta
     7261 gatgtaatgt tacagaatat atttttgttt cccaattgag taatactgtc tttttctgca
     7321 ttaattttac cgatcacact aatagataca gccaggagct gactagtatt aacacaatat
     7381 agttggcgga aatttgtaat aaagttaaga ataaaatcat ttagcctagg ataattttct
     7441 gctgagattg tataatcttg cttataaatt tctgcaccat tgagtttaca aagtgaaatt
     7501 tcaattttat gagatgaaac agtaacacaa agtagttgcc aaaaaaaaat tagaaacagc
     7561 taatccaacg gaggggcgac ctctggataa agtagtctga accgtacgtt ctaaaatgaa
     7621 gtggttatct attagtattt tagtcagatt tgttactgaa gctggggcta accccgatag
     7681 tttagctaaa tctgttcttg aaatgagttc aaattgctca attaaatggt ata
//
LOCUS       DANLZC010000116         7700 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.116, whole genome shotgun sequence.
ACCESSION   DANLZC010000116 DANLZC010000000
VERSION     DANLZC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.116"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            19..1446
                     /gene="hldE"
                     /locus_tag="PXE44_001983"
     CDS             19..1446
                     /gene="hldE"
                     /locus_tag="PXE44_001983"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDL5589464.1"
                     /translation="MMMQYSPKFNNAKVLVLGDVMLDRYWFGATNRISPEAPVPVVKV
                     QDIEERAGGAANVAMNIASLGVPVTLHGLIGDDDAGRALDKLLNSHQIQNHCVVINSH
                     PTITKLRILSRHQQLLRLDFEEGFHNVDSTALLAKLSQEITAYGALILSDYGKGTLES
                     VQQMIQIARNAKVPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGHCKDEDEIVSKGL
                     KLIADYDLSALLVTRSEKGMTLLRPNHEPYHLPTQAKEVYDVTGAGDTVISVLATAIA
                     DGRAYEEACYLANAAAGVVVGKLGTSTVSPVELENAIHQRTATGFGVVSEAELKAIVE
                     EAKKRGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNTDASVKRLKGEARPIN
                     DLAARMAVLAGLASVDWVVPFDEDTPQRLISEILPDLLVKGGDYKPEEIAGSQEVWAN
                     GGDVRVLNFENGCSTTNVIKKIQAL"
     gene            1460..2014
                     /gene="pyrR"
                     /locus_tag="PXE44_001984"
     CDS             1460..2014
                     /gene="pyrR"
                     /locus_tag="PXE44_001984"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL5589465.1"
                     /translation="MAREKIIIDTEQFQRTISRISHQIIEKHGDLQDVVIVGIKRRGA
                     EIAELIQKRIEDLAQTRLPYMALDITFYRDDLDLIYQDPVFMGADNQLNLNGKKVILV
                     DDVLFTGRTIRAALDALLDFGRAARIELVIFVDRGHRELPIRADYVGKNIPTAKNEQI
                     QVQTLSYDGINQVVLVPAANKESA"
     gene            2087..3037
                     /locus_tag="PXE44_001985"
     CDS             2087..3037
                     /locus_tag="PXE44_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL5589466.1"
                     /translation="MKMKLVKSLFAVAVATMSLTQTAHAFEERVVALVNDTPIMQSQV
                     QRVLGKKKATETAQRAAIDQVIDDMLVQQAMKEAGVKVSPAAVNQAVENVAIQNGITY
                     GQLLDALDYQGITLEQFKRNIAQQMAMEQVRHISISKSIQVAPQQVQALAKELMAKDK
                     AAGKLKTVSGKEHRISHILLKTNPILNDAQAKAKLSSLTTDINSGKISFEEAAKTHSV
                     DYASGADGGDLGFNFLEIYDPAFANVASKIKPNQISAPFKSQFGWHILKVTDTRNGDR
                     TEDAYHQRAYQQLVNKQAEEASKDWVKALRKTANIQYVGQ"
     gene            3099..3965
                     /gene="rsmA"
                     /locus_tag="PXE44_001986"
     CDS             3099..3965
                     /gene="rsmA"
                     /locus_tag="PXE44_001986"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL5589467.1"
                     /translation="MSSTNSKKHLGHTARKRFGQNFLHDMNIIHSIVSAINPKNGQFL
                     LEIGPGLGALTEPVAELVDKLTVVEIDRDLAERLRHHPFLNQKLTIIEQDALRFNFRD
                     YFNQLNLDEDSVRVFGNLPYNISTPLIFHLLTFHDLIQDMHFMLQKEVVKRLCAAPNS
                     KAYGRLTIMAQYYCQVIPVLEVPPTAFKPAPKVDSAVVRLVPYNQLPYPAKDVYWLNR
                     VTTQAFNQRRKTLRNALSTLFSAEQLDALGVDLTARAENLTIADYVRLANWLHDNPPA
                     IDTTEELVDEDC"
     gene            complement(4076..4705)
                     /gene="upp"
                     /locus_tag="PXE44_001987"
     CDS             complement(4076..4705)
                     /gene="upp"
                     /locus_tag="PXE44_001987"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL5589468.1"
                     /translation="MKIVEVKHPLVKHKLGLMREAEISTKDFRELANEIGSLLTYEAT
                     KDLEMESVEINGWSGEKIAVERIKGKKVTVVPILRAGLGMMDGVLEHIPSARISVVGI
                     YRDEETLKPVPYFSKLANDVDERLAIITDPMLATGGSMVATIDLLKKAGCKQIKVLVL
                     VAAPEGIKVLEAAHPDVELYTASIDSHLNENGYIIPGLGDAGDKIFGTK"
     gene            complement(4730..6415)
                     /gene="fadD"
                     /locus_tag="PXE44_001988"
     CDS             complement(4730..6415)
                     /gene="fadD"
                     /locus_tag="PXE44_001988"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDL5589469.1"
                     /translation="MEKIWLNNYPPYSPKTIETDQYESLVEMFEAAIQRHPDIPAYIN
                     MGQVLTFRKLEERSRSFAAYLQNELRLKKGDRIALMMPNLLQYPIALFGALRAGLVVV
                     NVNPLYTPRELEHQLNDSGAKAIVIVSNFAATLEKIVFNTEIKHVILTRMGDQLSFGK
                     RTLVNFVVKYIKKLVPKYKLPHAVSFREALSIGKQRQYVKPNIVSQDLAFLQYTGGTT
                     GVAKGAMLTHQNLIANMMQAKWIVEPLLGNSANMIGLVPLPLYHVLALSMNCLLFIEL
                     GLTGLLITNPRDIPGFIKELKKYKVSVITGVNTLFNALLNHESFKEVDFSHLKLSVGG
                     GAAIQSSVAKRWHDLTGVHIIEGYGMTECSPLISATRGDSVEYSGSIGVPVPNTDIRI
                     VDDEGKEVPLGERGELVVKGPQVMQGYWNRPDETAQVLQDGWMATGDIVVMGDDLNLR
                     IVDRKKDMIIVSGFNVYPTEIEEVISQNPKVNEVVAIGVPSEASGESIKIFVTKKDES
                     LTRDELRTYCRQFLTGYKIPRDIEFRDELPKSNVGKILRRVLRDEELAKVNKA"
     gene            complement(6393..6857)
                     /locus_tag="PXE44_001989"
     CDS             complement(6393..6857)
                     /locus_tag="PXE44_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDL5589470.1"
                     /translation="MMIEQATVVEYQNGVAIVQCYAKSSCGGCVAQSACGSKALSALA
                     GEKIDPRFRIEVDEPLKVGDKVELGLAENILLKSVFLIYGIPLLVLVMTAVGFSQFFA
                     NELIVLFIMLLSTGITFWAVRKLIHKHSKQANFSPIFLGKVLGDYNGKNMAK"
     gene            complement(6854..>7700)
                     /locus_tag="PXE44_001990"
     CDS             complement(6854..>7700)
                     /locus_tag="PXE44_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HDL5589471.1"
                     /translation="YELVYILQQGSDTESFRLRHAFTHNKEYAQLLNLDHSREEIILK
                     NNKVSYLGYNFRPFSLNSSQILDNLPNVLYTDYANLKGYAFLDAGKDRISDRITKVIR
                     MKPNDTLRYAYILWIDEETHLLLKSQVLNMDNLVLEEFRVLQLYQSPELDRIANVIES
                     LMLPTLTTVKKEEFPKSDGWQVKWLPTGFRLIQQQTVTGATYQVESEYVDSRVYSDGL
                     STLTIYIMPSQGVSFNEYAWQQGKLTILNQTINDRDIVIIGDVPLQSAKQIMNNIGFK
                     EGAQP"
BASE COUNT         2274 a         1671 c         1519 g         2236 t
ORIGIN      
        1 taacagagaa tattaactat gatgatgcaa tactctccaa aatttaataa tgctaaagtg
       61 ctggtgcttg gcgatgtaat gttagaccgt tactggttcg gggcaaccaa tcgaatttcg
      121 cctgaagccc ctgtgccggt ggtgaaagta caagatattg aagagcgtgc cggcggtgcg
      181 gcaaacgtgg cgatgaacat cgccagcctt ggcgtaccgg taacattaca cggcttaatt
      241 ggcgatgatg atgccgggcg tgctttagat aaattactta attcccacca gatccaaaat
      301 cattgtgtgg tgattaattc gcacccaacc atcacaaaat tgcggattct ttcccgccat
      361 cagcaattat tgcggttgga ttttgaagag ggttttcaca atgtagattc taccgcccta
      421 cttgcaaaac tttcgcaaga aattaccgct tacggagcat taattctctc ggattacggc
      481 aaaggcacat tggaatcggt gcaacaaatg atccaaattg cccgcaatgc taaggtgccg
      541 gtgctgatcg acccgaaagg cacagacttt gaacgctatc gtggggcaac attgcttaca
      601 ccaaatatgt cggaatttga agcggtggtt gggcattgta aagatgagga tgagattgtc
      661 agcaaagggt taaaactgat tgccgactat gatttatctg cactattagt tacccgttcg
      721 gaaaaaggaa tgaccttact tcgcccgaac cacgagcctt atcatctgcc gacccaagcc
      781 aaagaggttt atgatgtaac cggtgcaggc gataccgtaa tcagtgtatt agccactgcg
      841 attgccgatg gcagagccta tgaagaagcc tgctatttag ccaatgctgc cgccggtgta
      901 gtcgtaggta aattagggac ttcaaccgtc agcccggttg agctggaaaa tgcgattcac
      961 caacgcactg ccaccggttt tggcgtggtt tctgaagccg aattaaaagc gattgtggaa
     1021 gaagcgaaaa aacgtggcga aaaaatcgtg atgacgaacg gttgctttga tattcttcac
     1081 cccggtcacg tttcctattt ggaaaacgcc cgtaaattag gcgaccgatt aattgtagcc
     1141 gtcaataccg atgcttcggt aaaacgccta aaaggcgaag ctcgcccgat taatgattta
     1201 gctgcccgta tggcagtttt agctggttta gcctctgtag attgggttgt gccttttgat
     1261 gaagatacac cacaacgttt aatcagtgaa atcctacctg atttattggt aaaaggtggt
     1321 gattacaagc cggaagagat tgccggcagc caagaagtat gggcaaacgg cggcgatgtg
     1381 cgagtattga actttgaaaa cggctgttct accactaatg tgatcaagaa aattcaagca
     1441 ctataatggt aataagcgaa tggcgagaga aaaaatcatt attgataccg agcaatttca
     1501 gcgcacaata tctcgtattt cacatcaaat tattgaaaaa cacggtgatt tgcaagatgt
     1561 agtgattgtc ggcatcaaac gtcgaggagc tgaaattgcc gagttaatcc aaaaacgcat
     1621 tgaagattta gctcaaacac gcttgcctta tatggcgtta gatattacgt tctaccgtga
     1681 tgacctagac ttaatttatc aagatcccgt atttatggga gccgataatc agctaaatct
     1741 gaacggtaaa aaagtgattt tggttgatga tgttctgttt accggcagaa ccattcgggc
     1801 ggcacttgat gcactcttag attttggcag agctgccaga attgaactgg tgatttttgt
     1861 cgatagaggt catcgggaat tgcctatccg agccgattat gtcggaaaaa atattccaac
     1921 ggctaaaaac gagcaaattc aggtgcaaac gctctcgtat gatgggatta accaagtggt
     1981 cttagttcct gctgccaata aagaaagtgc ttaaattaag tgaacctttt cgctaaactt
     2041 atctctaagg aaaagttact gcttaattac ctccattagg attgttataa aaatgaaatt
     2101 agtcaaatca ttatttgctg ttgccgttgc aacaatgagt cttacccaaa ccgcccacgc
     2161 atttgaagaa agagtggtgg cgttagtgaa tgatacccct attatgcaaa gccaggttca
     2221 acgtgtgctt ggcaagaaaa aggcgacaga aactgctcaa cgtgcggcta ttgatcaagt
     2281 gattgatgat atgttggtgc aacaggcaat gaaagaagcc ggcgttaaag tcagcccagc
     2341 tgcggtaaac caagcggttg aaaatgtagc aattcaaaac ggcattactt acggtcagtt
     2401 attagatgcg ttagattatc aaggtattac acttgaacaa ttcaaacgta atattgctca
     2461 acaaatggca atggaacaag ttcgccatat cagcattagc aaatcgattc aggttgcacc
     2521 acagcaagtg caagctctgg cgaaagagtt aatggcgaaa gataaagcag caggcaaatt
     2581 gaaaaccgtt tctggcaaag agcatcgcat tagtcatatt ttgctcaaaa ccaacccgat
     2641 tttaaatgat gctcaagcca aagcaaaact tagcagctta actactgata ttaactcagg
     2701 caaaatcagc tttgaagaag cggcaaaaac ccattctgtt gattatgcct caggggcaga
     2761 cggtggagat ttaggtttta acttcttaga aatttacgat ccggcatttg cgaatgttgc
     2821 atcaaaaatc aaaccaaacc aaatttctgc tccgtttaaa tcgcaatttg gttggcatat
     2881 tttaaaagtc accgacactc gtaacggaga cagaaccgaa gatgcttacc accaacgtgc
     2941 ttatcagcaa ctggtgaaca agcaagccga agaagcctct aaagattggg taaaagcgct
     3001 tagaaaaacc gccaatattc aatatgtcgg tcaataatta aaatttttaa gaacctcgca
     3061 taatgcgggg ttcttttctt atttcaaaaa ggataacaat gagttctaca aattccaaaa
     3121 aacatttagg tcataccgct cgtaagcgtt tcgggcaaaa ctttttgcac gatatgaaca
     3181 ttattcacag catagtttca gccattaacc cgaaaaacgg acaattttta ttagaaatcg
     3241 gtccgggttt aggagcatta accgagccgg ttgccgaatt ggtagataaa ctcacggtgg
     3301 ttgaaattga ccgtgattta gcagagcgtt tacgccacca cccgttttta aaccaaaaac
     3361 tcaccattat tgagcaagat gccctacgct ttaatttccg tgactatttt aaccaactca
     3421 acttagacga agactcggtg cgtgtgtttg gtaacttacc ttacaacatt tctacgccct
     3481 tgatattcca cctgctgacc tttcacgact taattcaaga tatgcacttt atgttgcaaa
     3541 aagaggtggt gaaacgttta tgtgccgcgc caaacagtaa agcatacggg cgtttaacca
     3601 ttatggctca atactattgc caagtaattc cggtgctgga agtgccgcca accgccttta
     3661 aaccggcacc aaaagtcgat tctgcggtag tgcgtttagt gccttataac caactgcctt
     3721 atccggcaaa agatgtgtat tggctcaatc gggtcacgac tcaagccttt aaccaacgcc
     3781 gtaaaacctt acgcaatgct ctttcaaccc tgtttagtgc ggagcaactg gacgccttag
     3841 gcgttgattt aaccgcccga gcggaaaacc ttaccattgc cgattatgtg cgtttggcta
     3901 actggctaca cgacaacccg ccggcaattg atacaaccga agaattggtt gatgaagatt
     3961 gctaatcaat aaattacaag cggtcatttt tctaccaaga tttgcaaatc tttagtgaaa
     4021 aatggccgct tgtttaaatc agctttactt gttttgctgt tacttagttt tgatgttact
     4081 tggtgccgaa aattttatcc cctgcatcgc ctaagcctgg aatgatatag ccgttttcgt
     4141 ttaagtgact atcgattgag gcggtgtaga gttcaacatc tgggtgggcg gcttctaata
     4201 ctttaatccc ctctggagcg gcaactaaca ctagcacttt aatctgctta cagcctgctt
     4261 ttttcaggag gtcaatggtt gctaccattg agccaccggt tgcaagcatt gggtcggtga
     4321 taatggctaa acgctcatca acatcgttgg ctagtttgga gaaatacggc acgggcttta
     4381 aggtttcttc gtcacgataa atcccaacca cactaattct ggcacttgga atatgctcta
     4441 acacaccgtc catcatacct aagccagcac gcagaatcgg cacaacagtc acttttttgc
     4501 ctttaattcg ctcaaccgca attttctctc cgctccagcc attgatctcc acactttcca
     4561 tttccagatc tttggttgct tcgtaagtaa gcagactacc aatttcattc gctagctcac
     4621 ggaaatcttt ggtgctgatt tccgcttcac gcattaagcc caatttatgc tttacaagtg
     4681 ggtgttttac ttcaacaatt ttcattttaa gctccttaat tagtgtaatt taagccttat
     4741 tcacctttgc taattcttcg tctcttaaca ctcggcgaag aattttgccg acattacttt
     4801 ttggtaattc gtcacggaat tcgatgtctc ttggaatttt gtagccggtt aagaattgac
     4861 gacaataggt tctcaattca tctcgagtta aactttcatc ttttttggtt acaaaaattt
     4921 taatgctttc gccggaagcc tcactcggca caccaatcgc cactacttca ttcacttttg
     4981 ggttttggga aatcacttct tcaatttcgg ttgggtacac attaaaaccg gagacaataa
     5041 tcatatcttt tttacgatca acaatgcgta agttcagatc atcccccatt accacaatgt
     5101 caccggttgc catccaacca tcttgtagca cttgtgccgt ttcatcagga cggttccaat
     5161 agccttgcat tacttgcggc cctttcacca ccagctcgcc ccgttcgccg agtggtactt
     5221 ctttgccttc atcatccaca atacgaatgt cagtatttgg cactggcacg ccaatagagc
     5281 cggaatattc caccgaatcg ccacgggttg cactaatcag cggagaacat tctgtcattc
     5341 cataaccttc gataatatgt acgccggtta aatcgtgcca gcgttttgcc acgctagatt
     5401 ggatcgctgc accgccacct acacttaatt ttaggtgtga aaaatcaacc tctttaaaac
     5461 tttcgtgatt aagtaatgca ttaaacaatg tatttacccc agtaatcact gaaactttgt
     5521 acttttttaa ttctttaata aagcctggaa tatcgcgcgg attagtaatt aataatccgg
     5581 taagtcctaa ttcgataaag agcaagcaat tcatcgaaag tgctaagacg tgatacagcg
     5641 gtaaaggtac caatccaatc atattggcag aattacccaa caatggctca acaatccatt
     5701 ttgcctgcat catattagca attaagtttt gatgagtcag cattgcccct ttcgctacac
     5761 ctgtagtacc gccagtgtat tgtaaaaagg cgagatcttg gctgacaata ttcggtttta
     5821 catattgacg ctgtttgcca atgcttaagg cttcacggaa gctgaccgca tgtggcaatt
     5881 tatatttcgg cactaatttt ttgatgtatt tcaccacaaa attgactaag gtgcgtttgc
     5941 cgaatgaaag ttggtcgccc attcgggtca aaataacgtg cttaatctca gtattaaaga
     6001 caattttttc taaggtagcc gcgaagttcg agactatcac aatcgctttc gcaccactgt
     6061 catttaattg atgttctaat tcacgcggcg tataaagcgg gttgacattc accaccacaa
     6121 gcccggcacg taatgcgcca aataaagcga tcggatattg caataaattc ggcatcatca
     6181 atgcgattcg atcacctttt ttcaggcgta attcattttg caaataagcg gcaaacgagc
     6241 ggctacgttc ttccaattta cggaacgtta aaacctgccc catattaata taagctggaa
     6301 tatcagggtg gcgttgtatc gctgcttcaa acatttccac taaagattca tattgatctg
     6361 tttcaatcgt ttttggtgaa taaggcggat aattatttag ccatattttt tccattatag
     6421 tctcctaaaa ccttacctaa aaagataggt gaaaaatttg cttgtttgct gtgtttatga
     6481 atcaattttc ttacagccca gaatgttata cctgttgaaa gcaacattat aaataaaaca
     6541 ataagctcat ttgcaaaaaa ttgcgaaaat ccgaccgctg tcatcacaag aaccagcaga
     6601 ggaatgccat aaattaaaaa cacgcttttg agcaagatat tttcagctaa ccctagttca
     6661 accttatcgc ctacttttag gggttcatct acctcaatac gaaaacgagg gtcaattttt
     6721 tcacctgcta atgctgataa tgctttgctg ccacaagcag actgagctac acagccacca
     6781 cagctacttt tagcataaca ttgtacaata gccacaccat tctgatattc caccaccgta
     6841 gcctgttcga tcatcatggc tgagctcctt ccttaaaacc aatattgttc ataatttgct
     6901 tagccgattg caacggcaca tcaccaataa tcacaatatc tctatcatta atcgtttgat
     6961 ttaaaatcgt taatttacct tgttgccaag cgtactcatt aaaactcacg ccttggctag
     7021 gcataatata aatagttaat gtagataaac catcactata tactctgcta tctacatact
     7081 cactttccac ttgatatgtc gcacctgtga ctgtttgttg ctgaattaac ctaaaccctg
     7141 tcggcaacca cttcacctgc caaccatcag acttaggaaa ttcttctttt ttcactgtcg
     7201 ttaatgttgg taacattagt gattctatta catttgcaat cctatccaat tctggagatt
     7261 gataaagttg caaaactctg aattcttcta ataccaaatt atccatattc aagacttggc
     7321 ttttaagtaa taaatgtgtt tcttcatcaa tccatagtat ataggcatat cttaaagtat
     7381 catttggctt catacgaatt acctttgtaa ttcggtcaga aatacgatct ttaccagcat
     7441 ctaaaaaggc ataacctttt aaatttgcat aatctgtgta aagtacatta gggaggttat
     7501 ctaaaatctg tgaactattt aaactaaaag gacgaaaatt ataccccaaa tagctgacct
     7561 tgttattttt taaaataatt tcttctcggc tgtgatctaa attcagtaat tgggcatatt
     7621 ctttattatg ggtaaaagca tgacgtaaac ggaaagattc tgtatcactt ccctgttgaa
     7681 gaatataaac taattcataa
//
LOCUS       DANLZC010000117         7666 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.117, whole genome shotgun sequence.
ACCESSION   DANLZC010000117 DANLZC010000000
VERSION     DANLZC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.117"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            1..630
                     /locus_tag="PXE44_001991"
     CDS             1..630
                     /locus_tag="PXE44_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL5589472.1"
                     /translation="MAVSATSFAQTTQPTAAEQMNAVKASVAEKATVAKEATKEKAAE
                     LKAGSAEKMAIAKAKTESFKDSAKNKMDAVKATTAEKTDVIKAKAASASDTMKVKAQE
                     AKAATAEKAATAHETLNSAKTATAEKAASMKAAATEKMSAVKKSQKVNINTANEEQLQ
                     SLTGIGEAKAKAIVEYRKKHGKIKNAAELANIPGIGDATIEKVAPYLTF"
     gene            778..1407
                     /locus_tag="PXE44_001992"
     CDS             778..1407
                     /locus_tag="PXE44_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5589473.1"
                     /translation="MALDFNSVGLDSVEAKGGYGIGLQIGQQLLGSGMDVNAETVARG
                     IYDVLNNNEPAIDINEVTAALQELGQRAEAAQAEAFKAIDAENKAFLEENKKANGVIV
                     TDSGLQYEILTEGTGEKPTATSTVRVHYTGSLIDGTVFDSSVKRGQPAEFPVNGVIRG
                     WTEALQLMPVGSKWRLTIPQELAYGERGAEASIPPFATLVFEVELLDIL"
     gene            1473..1988
                     /locus_tag="PXE44_001993"
     CDS             1473..1988
                     /locus_tag="PXE44_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HDL5589474.1"
                     /translation="MQKKSLISRLLKNIFLYGSLFIIISLVVDWYRSPSAPAQFEQTV
                     HYDIQHQPKVIAQLSHEKPMLLYFWGSWCHYCQVVSPNIQQLANDGTEVLGVALKSGD
                     ENDVQAYLNENGYTFATLNDPSGDFSRGWNIQATPTILIIKDGKIAHHTTGYTSYLSL
                     KFRLWLADVLY"
     gene            complement(2036..2966)
                     /locus_tag="PXE44_001994"
                     /pseudo
     CDS             complement(2036..2966)
                     /locus_tag="PXE44_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433453.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3025..3675)
                     /locus_tag="PXE44_001995"
     CDS             complement(3025..3675)
                     /locus_tag="PXE44_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha4 family transposase"
                     /protein_id="HDL5589475.1"
                     /translation="MRKSRLSQYKQNKLIEQFVAGVTARTASELVNVNKNTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEVDESYLGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQFATLLPIIREKVKPDSIVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFGSQAKRHLRKFNGIPKVHFELYLKECEWRFNHSDLKSQISFLKQLVKGSLG"
     gene            4004..5362
                     /gene="mnmE"
                     /locus_tag="PXE44_001996"
     CDS             4004..5362
                     /gene="mnmE"
                     /locus_tag="PXE44_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL5589476.1"
                     /translation="MKDTIVAQATPIGRGGVGILRVSGPLAETVAQAVLGKTLPPRIA
                     NYLPFKDEDGTVLDQGIALFFKAPNSFTGEDVLELQGHGGQVILDLLLKRILQVKGLR
                     IARAGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAARSALKSLQGEFSNKINALVDS
                     VIYLRTYVEAAIDFPDEEIDFLADGKIEAKLNEIIAQLDGVRREAKQGSILREGMKVV
                     IAGRPNAGKSSLLNALAGREAAIVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAS
                     DEVERIGIQRAWEEIAQADHVLLMIDSTEQQADQFRQEWAEFLAKLPANMPVTVIRNK
                     VDLSGEQEGLIQVDDFTMIRLSAQTKVGVDLLREHLKKSMGYQSSTEGGFLARRRHLQ
                     ALETAAEHLERGHIQLTQFLAGELLAEELRMVQNALSEITGQFTSDDLLGNIFSSFCI
                     GK"
     gene            5363..6628
                     /gene="tilS"
                     /locus_tag="PXE44_001997"
     CDS             5363..6628
                     /gene="tilS"
                     /locus_tag="PXE44_001997"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL5589477.1"
                     /translation="MALFNRFQTQCATHLPNQTAFLIGLSGGVDSVVLLHLFARTSFH
                     VRAIYVHHGLSPNADDWAAFCEQYCKRLNIPFILQKVRVDSSNGVENGAREARYQAIQ
                     QQLKPNEVFATAHHLDDQAETFLLALKRGSGVKGLSAMQAVTFLQNFTVFRPLLTFTK
                     SDLIGYAVQHQLGWIEDESNADNRYDRNFLRNSILPLLNQRWQHFSQMVARSAQHCAE
                     QQALIEELLSNELKSRTGEKQQLNINGFGQFSLAKQQQLSRLWLEQNGVRMPSQAQLQ
                     AVISELIFAKADKNPQVKVGENIIRRYQQAIYVTEEIADIPPFEVVLPTETKVELPYQ
                     LGTIIRQQQEIICKKNGKNHRLQLPQELAQSTISIKIGQLGKVKCYGKAQREEMKKIW
                     QNHGIPVWERNRTPLIFWQDELVGLLPIL"
     gene            complement(6618..7511)
                     /gene="xerD"
                     /locus_tag="PXE44_001998"
     CDS             complement(6618..7511)
                     /gene="xerD"
                     /locus_tag="PXE44_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL5589478.1"
                     /translation="MAELDPIIEQFLDTLWQEHGLSENTVASYRSDLERFSDYFSEPK
                     AFLSLDHIDLQGFLGERLEQGYKATSSARMLSCLRKFFRFLCLEHYRQDDPTLTLSSP
                     KRGASLPKSLSEDQVMDLLDAPNTLDPIELRDKAMLELLYATGLRVTELVSLSMDNIS
                     LKQGVVRIIGKGDKERLVPLGEEANYWIQEFFQYGRPILLNNQQSDVVFPSRRGQQMT
                     RQTFWHRIKHYAVLAGIDADKLSPHVLRHAFATHLVNHGADLRVVQMLLGHSDLSTTQ
                     IYTQVAKARLKSLHQQFHPRG"
BASE COUNT         2369 a         1753 c         1641 g         1903 t
ORIGIN      
        1 atggctgttt cagctacttc atttgcacaa accactcaac ctactgcggc tgaacaaatg
       61 aacgcagtta aagcctctgt tgccgaaaaa gcaactgtgg ctaaagaagc aacaaaagag
      121 aaagcagcgg agttaaaagc cggttctgcg gaaaaaatgg cgatagcaaa agccaaaacc
      181 gaatcattta aagattccgc taaaaacaaa atggacgcag taaaagccac cacagcagaa
      241 aaaacggacg tgattaaagc gaaagccgca tctgcaagcg acacaatgaa agtgaaagct
      301 caggaagcaa aagccgccac cgcagagaaa gctgcaactg ctcatgaaac cttaaattca
      361 gccaaaaccg caaccgctga aaaagcggcc tcaatgaaag cagctgcaac agaaaaaatg
      421 tcagcagtga aaaaatcaca aaaagtgaat atcaacactg ccaacgaaga gcaattacaa
      481 tcgcttaccg gcattggtga agcaaaagcc aaagcgattg tagaataccg caaaaaacac
      541 ggtaaaatca aaaatgcagc agaactggcg aacattcccg gcattggcga tgcaaccatt
      601 gagaaagttg ctccatattt aactttctaa tgttctgcaa gcggttattt tgttgcaaaa
      661 ttttgcagaa aaataaccgc ttgttcattt cacaccttgt attcttaaat ttcaccccca
      721 ttaagtgaat cgtatttctt tcaatacagt attcaataaa ctcaaaagga acagaaaatg
      781 gcattagatt tcaattcagt tggattagat tcagtagaag caaaaggcgg ttacggcatc
      841 ggtttacaaa ttggtcagca acttttaggc agcggaatgg acgtaaacgc agaaaccgtt
      901 gcccgtggta tttatgatgt gttaaacaac aatgagccgg caatcgacat caatgaagtg
      961 accgctgcat tacaagaatt aggtcagcgt gcggaagcgg ctcaagcaga agcattcaaa
     1021 gcgattgatg ccgaaaacaa agcgttcttg gaagagaata aaaaagcaaa cggtgtaatc
     1081 gttaccgaca gcggtttaca atatgaaatt ttaaccgaag gcacaggcga aaaaccgact
     1141 gcgacttcaa ccgtgcgtgt tcactacacc ggttcattaa ttgacggcac cgtgtttgac
     1201 agctcggtaa aacgcggtca gccggcagaa ttcccggtaa acggagtaat tcgtggttgg
     1261 acagaagcat tgcaattaat gccggttggc tcaaaatggc gtttaaccat tccacaagaa
     1321 ttagcctacg gcgaacgtgg tgcagaagca tcaattccac catttgcgac cttagtgttt
     1381 gaagtggaat tgcttgatat tctctaatca aactcaaata aaaaacctgt ggaggcattt
     1441 cacaggtttt tgctatttaa gataactata ttatgcagaa aaaatcactc atatctcgct
     1501 tacttaaaaa tattttcctc tacggcagcc tgtttatcat tattagcctg gtggttgatt
     1561 ggtatcgctc gccctccgct ccggcacaat ttgagcaaac cgtgcattat gatattcaac
     1621 accagccgaa agtaatcgcc caattaagcc acgagaagcc gatgctactc tatttttggg
     1681 gaagttggtg ccactattgc caagtggttt cgccaaatat ccaacaatta gcgaatgacg
     1741 gcacggaagt tttaggtgtt gccctgaaat ccggcgatga aaatgacgta caagcctatc
     1801 tcaatgaaaa tggctacact ttcgctactt tgaatgaccc aagcggtgat ttctctcgag
     1861 gttggaacat tcaagccacc ccaaccatct tgattatcaa agacggtaaa atcgcccacc
     1921 acaccaccgg ctacactagc tatttgagct taaaattcag actatggctg gcagacgtgt
     1981 tgtattaaac gattcctttc atttgcaaaa aaatccacta aatccgaccg cttgtttaca
     2041 ccaaaaagac aagcggtact aaattcattg tcatcattgt ggtgatcgat aaaatcagcc
     2101 tcgatgagcc aaaaataaag cctcggtttt tgccgttgtc gaatttcacc agtaaatttg
     2161 ccattgctgt gagggcgtac acttccacca ccgtaaaaat cacggtgtaa atataggcag
     2221 cggtcagagt tgcgactttt accgcaataa accaaggggc aataatcgcc accgctaata
     2281 acggcgaaat ccaaattaat tgctgtttgg tgaggttgat tttacgagaa aaagtcgatt
     2341 gcaaactgac ggttaaaaca ccgttgatga ataacgcaat cggcgaatgc tccggtgcgt
     2401 gcaggacatt gtcgaataaa tacggaaaaa tcactcagcg gcacaaatag cattaagtga
     2461 gcgaacaaaa actctttgtt gatgatttcc ttaatttgtg cgaaacgaaa tttcacgccg
     2521 ccattcacgc cttccaccga ataaaacggc ttgaccataa acgcaaacaa gccggcttcc
     2581 aaaataaagc agatccaaat cagcgtttca atcatctcaa acctaataaa cggaataata
     2641 atcagcgggg cgagcattga agacatactg gtgactttta gatatttgcc ttgtaaacgg
     2701 gttttagcag catattcatc gccggcaatc gccagtaaac aggctctggc attagtgccg
     2761 aataagcaac tccccaaccc aaagcaagtg gttgccaaca ataaaatgag gtaattatcc
     2821 gctgctaaaa acagcatata ggcaaccaca tctaataaac agcccaataa catcattttt
     2881 gccaagccgt agcgatcccc ccaaatgccg gctaaaattg ccaatgcctg attacaaaac
     2941 aacagcaatg aaaggaaaaa agcgatttgg gcgttatcta gccctttctc ttttaaataa
     3001 aagaaaaggg gctgtcctag ataactaacc taaactaccc ttaaccaatt gttttaaaaa
     3061 ggatatttga gattttaagt cactgtgatt aaaacgccat tcgcattctt tcaaatacag
     3121 ctcaaaatgc actttgggaa tgccgttaaa cttacgcaaa tggcgttttg cttggctccc
     3181 aaaattctca attccattaa tgtgattttg attttcagcg aagtgtgtgc tgtgattaat
     3241 gcgaaaatgg ttaaattcac tcacatcaag cacatcataa cttcgataga aatcggtgta
     3301 aacaatacta tcgggtttca ccttttcccg aataatcggc aaaagtgtgg caaattgcgt
     3361 gtttggtact accacggtat aaacctttcc gtcccgcttt aaaagcccga acacggcggt
     3421 tttgcctgct gcaccacgtc cacgtttacc tttacgatga ccgcctaaat aactctcatc
     3481 aacctcaatt tctccctcaa acatttccaa atgagggcta tgttgataga taagcaaccg
     3541 caagcgatgg aagtaatacg ccgccgtgtt tttgtttaca ttgaccaact cagatgctgt
     3601 tcgggctgtt acccctgcaa caaactgctc aatgagtttg ttttgtttgt actgacttag
     3661 acgacttttt ctcatttggg tatcttaacc taaatggagt ttttagtcgt tatctaggac
     3721 agccccaaag aaaaacacgc tttgcattga aaaaaaggcg gtggtggaaa taaaacggcg
     3781 taggagtaat agtttttcta aagacattca gaatggttga attggttaaa taaagagcgg
     3841 ttattctacc ttttaagtca accccaatca atcggctttt gttttacctt tggcgtgggt
     3901 ttcggtatga taagcgatat gtagtggcgg gtcctgtgcc cgcctaaata ccaatgataa
     3961 cttgcgggcg agcacaggac tcgcccctac gataaacaag aaaatgaaag acaccattgt
     4021 tgctcaagca acaccgattg gcagaggcgg tgtggggatt ctgcgagttt ccggtccgct
     4081 tgccgaaacc gttgctcaag ccgtattagg caaaacattg ccaccccgta tcgcaaatta
     4141 tttgccgttt aaagacgaag atggcactgt gttggatcaa ggcatcgcct tattttttaa
     4201 agctcccaac tcctttaccg gcgaagatgt gcttgaactt caaggacacg gtgggcaggt
     4261 tattttagat ttactgctca aacgcatttt acaagttaag gggctacgca ttgcccgagc
     4321 aggtgaattt tccgaacagg catttttaaa cgataagctc gaccttgccc aagctgaagc
     4381 gattgcggat ttaatcgacg ccaccagcga gcaagccgct cgttcagccc tgaaatcact
     4441 acagggtgaa ttttctaata aaatcaatgc gttagttgat tcagtaattt atttacgcac
     4501 ttatgtggaa gcggcgattg acttccccga tgaggaaatt gactttctgg cagacggtaa
     4561 aatcgaggca aaattaaatg agattatcgc tcaattagac ggtgttcgcc gtgaagcgaa
     4621 acaaggctcg attctgcgtg aaggaatgaa agtagtgatc gccggtcgcc cgaatgcagg
     4681 gaaatcaagc ttgcttaacg ccttagccgg acgagaggcg gcgattgtaa ccaatattgc
     4741 tggcacaacc cgtgatgtgt tgcgtgaaca tattcacatt gacggaatgc cgttgcatat
     4801 tatcgacacc gccggtctcc gtgatgccag tgatgaggtg gaacgtatcg gcattcagcg
     4861 tgcttgggaa gaaatcgccc aagccgacca cgttttatta atgattgaca gcaccgagca
     4921 acaagccgat cagttccgac aagagtgggc ggaatttcta gccaaactgc cggcaaatat
     4981 gcctgtgacc gtaattcgca acaaagtcga tttatccggc gagcaggaag gcttaattca
     5041 ggtggacgat ttcacgatga ttcgcctctc tgcccaaacc aaagtcggcg ttgatttatt
     5101 gcgtgaacac ttgaagaaat caatgggcta ccaaagttct acggaaggcg gattcttggc
     5161 tcgccgccgt catctgcaag cattggaaac cgctgccgag catttagagc gagggcatat
     5221 ccagcttact caattcttag ccggcgaatt gttagcggaa gagttgcgta tggtgcaaaa
     5281 tgccttaagt gaaattaccg gccagttcac ctctgatgac ttattaggca acattttcag
     5341 ctcattttgc atcggtaaat aaatggcact ttttaaccgc tttcaaaccc agtgtgcaac
     5401 gcatttgccg aaccaaaccg cctttttaat cggcttaagc ggtggggtgg attcggtggt
     5461 actactccac ctttttgccc gcacctcatt tcacgttagg gcaatttatg tccaccacgg
     5521 gctaagccca aatgcggacg actgggcagc cttttgcgaa caatattgca agcggttaaa
     5581 tattcccttt attttacaaa aggtcagagt ggatagctca aacggcgttg aaaacggggc
     5641 gagagaagct cgttaccaag ccattcaaca acagcttaag cccaatgaag tgttcgccac
     5701 cgcccaccat ttagatgacc aagccgaaac ttttttgctc gccctcaagc gaggcagtgg
     5761 agtcaaagga ttatcggcga tgcaagcggt cacttttttg caaaatttta ccgtttttcg
     5821 accgctactc acttttacta agtcagattt gataggttat gccgttcaac atcagctagg
     5881 ttggattgag gacgaaagca atgccgataa ccgttacgac cgtaatttcc ttcgcaatag
     5941 catcttgcca ctactcaatc aacgttggca gcacttcagc caaatggttg cccgttccgc
     6001 tcagcattgt gcagaacagc aagccttgat agaagagcta ttaagcaatg aactcaagtc
     6061 aagaacaggg gaaaaacaac aactcaacat taacggcttt gggcaatttt cacttgccaa
     6121 gcaacagcaa cttagccgcc tttggctgga acaaaacgga gtgagaatgc caagccaagc
     6181 ccagctacaa gcggtcattt ctgaactgat ttttgcaaaa gccgataaaa acccacaggt
     6241 taaagtcggc gaaaatatta tcagacgcta tcaacaagct atttacgtta ccgaagagat
     6301 cgccgacatt ccgccgtttg aggtagtact tcccacagaa accaaagtgg aattacccta
     6361 tcagctcggt actattattc gccaacaaca agagatcatt tgcaaaaaaa acgggaaaaa
     6421 tcaccgcttg caactgcccc aagagctggc tcaatcaact atttctatta aaatcgggca
     6481 actaggcaaa gtaaaatgct acggcaaggc tcaacgggaa gaaatgaaaa agatttggca
     6541 aaatcacggc atacctgttt gggaacgcaa ccgtacaccg ctgatttttt ggcaagacga
     6601 attagttggc ctattaccta tcctctaggg tgaaattgct gatgaagcga tttcaaacgg
     6661 gcttttgcca cttgagtgta aatctgggtg gtcgataaat cactatgccc cagcaacatc
     6721 tgtaccactc gcaaatcagc cccatgattg accaaatggg tcgcaaaggc gtggcgaagc
     6781 acgtgaggcg agagtttatc tgcatcaata cctgcaagca cggcatagtg cttaatccga
     6841 tgccaaaacg tctgccttgt catctgctga cctcgccggc tcggaaatac aacatcagac
     6901 tgctgattat tcagcaaaat agggcgaccg tactggaaaa actcctgtat ccaataattc
     6961 gcctcctcgc ccaatggcac taaccgctct ttatcccctt taccgataat tctcaccacc
     7021 ccttgtttga ggctgatatt atccatcgaa agcgacacca gctccgttac ccgcagaccg
     7081 gtggcataca gcagctccag cattgcttta tcccgtaact ctatcggatc taacgtattc
     7141 ggggcatcta gcaaatccat tacctgatct tcacttaagg atttgggtaa agaagcaccc
     7201 cgtttagggg aagaaagggt taaagtcggg tcatcttggc gataatgctc aaggcataaa
     7261 aaacggaaaa atttccttaa acaactcaac attctggcag agcttgtcgc cttatagcct
     7321 tgctccaaac gctcgccgag aaaaccttgt aagtcgatat gatcgagaga cagaaatgct
     7381 ttaggttcag aaaaataatc ggaaaaacgc tctaagtcag aacgataaga ggcaacggta
     7441 ttttcagaca aaccgtgttc ttgccatagt gtatcgagaa attgttcaat aatagggtca
     7501 agctcagcca tacaagcggg tatattttca ggaaattttg caaattttaa cagaattaaa
     7561 tctataaaaa tacaaaagcg atagaaacga aaaaaccgat tagcttatgc taatcggttt
     7621 aaattattgg cggaacggac gggactcgaa cccgcgaccc cctgcg
//
LOCUS       DANLZC010000118         7609 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.118, whole genome shotgun sequence.
ACCESSION   DANLZC010000118 DANLZC010000000
VERSION     DANLZC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.118"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            31..774
                     /locus_tag="PXE44_001999"
     CDS             31..774
                     /locus_tag="PXE44_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5589479.1"
                     /translation="MFEFIKLITAMILPPFNIILLWLLALILFSLGYKYAAYFFALLG
                     ILILYIFSIPYTSQKLGDSLVEAEPFTIEKYKNAQAIVVLGGGLRDSQELFGKLAITG
                     IPLERMRYAAYLHKETGLPILATGSSPNGTSEAKVMADEFKMFFNIETKWLENKAKTT
                     KENAIFSREILEKENIHKIILVTNQWHMKRAEMLFSQQGFEVMSASVGSGDTPDHYKL
                     NFMHFIPQAGAMNSNMLALKEWIGYWKEK"
     gene            complement(851..3049)
                     /gene="priA"
                     /locus_tag="PXE44_002000"
     CDS             complement(851..3049)
                     /gene="priA"
                     /locus_tag="PXE44_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL5589480.1"
                     /translation="MALNVPLHRYFDYLLPPAFNACQGGRVVVPFGRQTKIGIVVDFP
                     QTSDVPTEKLKPIKAALDSSPIFDSEMWQLLNWAARYYHAPIGEVLTGALPIKLRNGD
                     STERVQPNYFVVTEQGRKSLIIGENKKAKKQVERLTELAECAKYFEKPTACSPSAWKG
                     LLEKGYIEKIDVPFMAQAWQDSLGEFPLVNTNNRLILNKQQTLVVSRLNAQTTFASFL
                     LNGVTGSGKTEVYLQLIETVLKREEQVLLLVPEIGLTPQTVQRFKARFNVEIDVLHSN
                     MNETERLNAWLRAKNGESAIVIGTRSALFTQFQKLGVIILDEEHDSSFKQQDGWRYHA
                     RDLAVVRAKNANIPIVLGSATPSLESLHNVQNGKFIELTLTARAGNAQLANQQIIDLK
                     TQRVTAGLSERLLSMMKTQLEQGNQVMLFLNRRGFAPVLLCHECGWVCECEACNKPFT
                     YHQKQKVLRCHHCATQRVIPRQCGHCGSTHLITTGVGTEQLEQVLNQQFPHYQVTRID
                     RDSTARKGALENHLTAIREGKSQILIGTQMLAKGHHFPNVTLVAIVNVDSALFSTDFR
                     AEEKLAQLYIQVAGRAGRAEKKGEVVLQTHYPDHPLLKTLLEQGYRAFADEALKMRQL
                     MSLPPFSSQVLFKATGKDNQQTLQLLEQLADYFSQKIQQQNGQGFQLPPPFSAPMAKK
                     AGHYRWLLLIQHPLRGALQQLLDAFDQERESLNIPNNIRLNIDIDPQEIS"
     gene            complement(3061..3673)
                     /locus_tag="PXE44_002001"
                     /pseudo
     CDS             complement(3061..3673)
                     /locus_tag="PXE44_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            complement(3666..4565)
                     /gene="rarD"
                     /locus_tag="PXE44_002002"
     CDS             complement(3666..4565)
                     /gene="rarD"
                     /locus_tag="PXE44_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5589481.1"
                     /translation="MLKGILLSLSASVLFGCMYYLAIFLKPLAGVSIFGIRMLVTIPF
                     LFLALYLFKKQNEFWVFLQRLKQEPYLLLVILITASIVGGQMWLFLWAPNSGKAIEVS
                     MGYLLMPIIMVAFGKVVYKETLSLNKWLAIVVAFIGVASNILFTGKISIASLFVCTGY
                     PIYFYLRRKFGLSHLHSFVVEILYLLPVACYFISQTDMQYVETQNLNIYYFIALLGLI
                     SGTALISYTLASTILPFNLLGLLGYVEPLLMLVVSFLIGERLANESYLLMICLLISIS
                     FLVLDGMFALRQQRKRKLSVNNE"
     gene            complement(4792..6009)
                     /locus_tag="PXE44_002003"
     CDS             complement(4792..6009)
                     /locus_tag="PXE44_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL5589482.1"
                     /translation="MDRFPKSDKLAQVRYDIRGPIHKEALRLEEEGHKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIVQYYQSKGMRGMDVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPMPDYPLWTAASTLAGGKAVHYLCDEENEWFPDLED
                     IKSKITPRTKGILVINPNNPTGAVYSRQILLEIAELARQHNLIIFADEIYEKIVYDGA
                     VHHHIAALAPDVLTVTYNGLSKAYRVAGFRQGWMVLSGPKHQAKGFIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFVLPGGRLLEQRNKMHELLVQIPGISCVKAKGALYM
                     FPKIDTEMYGIKDDQKFIYDLLRQEKVLLVQGSGFNWKKPDHFRVVTLPYAHQIEEAI
                     GRLANFLKTYRQD"
     gene            6197..7501
                     /locus_tag="PXE44_002004"
     CDS             6197..7501
                     /locus_tag="PXE44_002004"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HDL5589483.1"
                     /translation="MLIFHQLKQELTKSYSKVGREQGLVAIQASMPLLEANIPLLGWL
                     KTQKTSYPHFFLQYRDTDQTIATIGTLRQFYTLEAAQSFIQQHHFPLIGGLQFEGNTQ
                     FILPQFSLVKNQQNLTACFYFDSDNFEQQAVIFEQFLANFSQITELGLTDNPLVSMSA
                     TSHFEQWKANIEKAIVSIEQGDFQKVVLANAMRLHFEQPICAYSLLAQSQKTNLGCYH
                     FLWAESADVAFLGSTPERLYQRQGLHFSTEALAGTAAVTTSEVQTELNAQWLLSDPKN
                     IYENQLVVDDIENQLKDCVTEFAVSEAKIKRLHNVQHLRRQIQATLKPDTSDSDCLSK
                     IHPTAAVAGLPRQKAKGFITENEPFTRHWYAGTLGVMGNEEAEFCVTLRSALISQNSI
                     TLYAGAGIVSGSDPQSEWQEIKRKAQGMAKLLDVDLVTKGEL"
BASE COUNT         2296 a         1668 c         1491 g         2154 t
ORIGIN      
        1 tttacttatt tttactacga tattctttct atgttcgagt tcattaaact gatcaccgca
       61 atgatcctcc cgccatttaa tattattttg ctgtggttgt tagcattaat cctcttttct
      121 ttaggctata aatacgctgc ttacttcttt gccttgttag gtattctgat tttatatatc
      181 ttcagcattc cctatacttc acagaaatta ggggattcat tagtcgaagc agagccattt
      241 accatagaga aatataaaaa tgctcaagcg attgtggtgc taggtggcgg gctacgagac
      301 agccaagagt tattcggcaa actggcaatt accggtattc cgctggaaag aatgcgttat
      361 gccgcttatc tgcataaaga aaccggctta ccgattttag cgaccggaag cagcccaaat
      421 gggacttctg aggcaaaggt tatggcagat gaatttaaaa tgttcttcaa tattgagacc
      481 aaatggctgg aaaataaggc gaaaaccacc aaagaaaatg cgattttcag ccgtgagatt
      541 ttagaaaaag agaatattca taaaatcatt cttgttacca accaatggca tatgaaacgg
      601 gctgaaatgc tgtttagcca acaaggattt gaggtgatgt ctgccagcgt cggttctggc
      661 gatacgcccg atcattacaa actcaacttt atgcacttta ttccgcaagc cggtgcaatg
      721 aatagcaata tgctggcatt aaaagagtgg attggttatt ggaaagagaa ataaccgaag
      781 ataaatagca gcaaggcagg ttttcacctg ccttcttttt gcaaaaaatc agtcaaaaac
      841 aaccgcttgt ctaactaatc tcttgtgggt caatgtcgat attcagcctg atattattcg
      901 gaatgtttaa actttccctt tcttgatcaa aggcatcgag tagctgttgt aatgctcctc
      961 ggagagggtg ttgaatcagc aataaccagc ggtaatgtcc tgctttcttc gccattgggg
     1021 ctgaaaatgg tggagggagc tggaagcctt gcccgttttg ttgctggatt ttttggctaa
     1081 aataatctgc taattgctca agcagctgta aggtttgttg attatctttg ccggtcgctt
     1141 tgaacaaaac ttgcgaggaa aatggtggca aactcattaa ttgcctcatt tttaacgcct
     1201 catcagcaaa agctcggtag ccttgttcca gcaaggtttt aagcagtggg tgatcagggt
     1261 aatgggtttg tagcaccact tcgccttttt tctccgctcg ccctgctctg cctgccacct
     1321 gtatgtaaag ctgtgctaat ttttcttccg cacgaaaatc agttgaaaat aacgctgaat
     1381 ccacattaac aatcgccacc aacgtcacat tcgggaaatg atgccctttt gccaacattt
     1441 gcgtaccaat caaaatttgg cttttgccct ctcgaatagc ggtcaaatgg ttttccaatg
     1501 cacctttgcg agcggtagaa tctctgtcta tacgagtcac ttggtaatga gggaattgct
     1561 gatttagcac ctgctcaagt tgttctgtgc cgacaccggt agttattaaa tgggttgaac
     1621 cacaatgtcc gcattggcgg ggaattaccc tttgggtggc acaatgatgg caacgcaaca
     1681 ctttttgctt ttggtggtaa gtaaaaggct tattacacgc ctcgcactca caaacccaac
     1741 cgcattcgtg acaaagtaat accggggcaa agccacggcg atttaaaaac agcatcactt
     1801 gattgccttg ctctaactgg gttttcatca ttgaaagcaa gcgctccgac aaaccggcag
     1861 ttacccgttg cgtttttaaa tcaataatct gctgattggc aagttgtgca ttgccggccc
     1921 gagcggttag tgtcagttcg ataaatttcc cgttttgcac attgtgcaaa ctttcaagac
     1981 ttggcgttgc cgagcccagc acaatcggaa tatttgcatt tttcgcccta accaccgcca
     2041 aatcccgtgc gtggtaacgc caaccgtctt gttgcttaaa cgagctatcg tgttcttcat
     2101 ccaagataat cacgcccaat ttttgaaact gggtaaataa agcagagcga gtgccgatta
     2161 caatcgcact ttctccattt ttcgcccgca accacgcatt taaccgctca gtttcgttca
     2221 tattcgagtg caatacgtca atttcaacgt taaagcgagc tttaaagcgt tgtaccgttt
     2281 gcggtgttag cccgatttca ggcacaagca gcagcacttg ttcctctcgc ttaagcaccg
     2341 tttcaattaa ctgcaaatac acttcggttt tgcccgagcc tgttacgcca ttgagtaaaa
     2401 agctcgcaaa agtggtttgt gcatttaaac ggctgaccac taaggtttgt tgtttattta
     2461 agatcagccg attgttagta tttactaacg gaaattcacc tagtgagtct tgccacgctt
     2521 gtgccataaa cggcacatca attttctcga tatacccttt ttcgagtaag cctttccaag
     2581 cagatgggct acaagcggtc ggtttttcaa aatattttgc acattcggca agctcggtta
     2641 accgttccac ctgctttttg gcttttttat tttcaccgat aatcagagat ttccgccctt
     2701 gctccgtcac cacaaaataa ttcggttgaa ctcgctcagt ggaatcgcca ttgcgtaatt
     2761 ttatcggtaa ggctccggtt agcacttcac caatcggggc gtggtaataa cgagccgccc
     2821 agttcagcag ttgccacatt tctgagtcaa aaatcgggct agaatccaac gctgctttaa
     2881 tcggtttgag tttttcggtt ggcacatcac tagtttgcgg gaaatcgacc acaatgccga
     2941 ttttggtctg gcgaccaaaa ggcacaacca cccgcccgcc ttggcaggca ttgaatgccg
     3001 gcggtagcaa ataatcaaaa taacgatgta acggcacatt cagtgccact ctgattaatc
     3061 tcacttctct aaaccaattt tgattgcaag cccgataaat atcgagccgc agattttatt
     3121 taaccaattt ccaaaggttt gattatgccg taaaaaacgc ccaacaggtg cagcggctaa
     3181 gactaaaata cttacccaaa taatgctggt ggataaaaac gttagcccca acagcataaa
     3241 aggtattttc caaattactg gatacaaact gcggcaataa cgccaagaaa aagagcaata
     3301 ctttcgggtt taatagattg gtaatcaacc cttgacggaa tatcttccat agaggaagtt
     3361 tgtctagatt gattttctct aacaccaacg gctctcgcca cattttaatg ccgagatagc
     3421 agagataaat tgcccctgcg tatttcagaa aattaaataa agtgggcgaa tgtgccacaa
     3481 tgcctgcaag cccgagtgaa accgcaagaa tatggcataa aatgcctgct agaatgccga
     3541 atgaagacat taagcccgac tttttcccct cagcaatagt gcgagttaaa atatagatag
     3601 tatccgaacc cggtgtaaga tttagcagaa tacaagcggt caaaaatccc caataattta
     3661 caatatcact cattattcac actcaattta cgtttacgct gctgacgaag agcaaacata
     3721 ccatctaata ctaaaaatga aatggagatc aacaagcaaa tcatcaacaa gtaagattca
     3781 tttgctaacc tttcgccaat taagaaagac accaccaaca tcaatagtgg ctcaacatag
     3841 cctaataaac caagcaaatt aaagggtaaa atcgtgctgg ctagcgtata gctgataagt
     3901 gcagtgccac taattaaacc cagtaatgca ataaaatagt aaatattgag gttttgtgtt
     3961 tcgacatatt gcatatcggt ttgagagata aaataacaag ctacagggag caaatataaa
     4021 atttctacca caaagctatg taaatggctt aagccaaact ttcggcgaag gtaaaaatag
     4081 attgggtaac ctgtgcaaac aaataaactt gctatcgaaa tttttcccgt aaacaaaata
     4141 ttactcgcta caccaatgaa agcaaccaca atcgccaacc atttattcaa ggaaagcgtt
     4201 tctttataaa ccactttgcc aaatgccacc ataataatcg gcattaatag atagcccatt
     4261 gaaacctcaa tcgcttttcc gctattggga gcccataaaa acagccacat ctgcccgccc
     4321 acaatagatg ccgtgattaa aataaccagc aataaataag gctcttgctt taggcgttga
     4381 agaaataccc aaaactcatt ttgtttttta aataagtata aagcaaggaa aagaaaagga
     4441 atcgtaacca gcatacgaat gccgaaaatg ctcacacctg ccagcggttt tagaaagatc
     4501 gccaaatagt acatacagcc gaataacact gaggctgaaa gagataaaag aatgcctttt
     4561 aacatagtga taagaaagta attatttttt aatttttcaa atctccccta cccctctttt
     4621 ctaaagagga ttgtcgagtt ctaaataaat gaataatgtt cggaggtagg ggcgggtcca
     4681 gtgcccgccc gcaaattaat atcgttagcg gacaggcaca ggacctgtcc ctactgatat
     4741 tatagaactt acaaccctgc ttttataaag tggggtaaag ggagattcga cttagtcttg
     4801 acgataggtt ttcaagaaat tcgcaaggcg accaatcgct tcttcaattt gatgtgcata
     4861 aggcaaagtt accacacgga agtgatccgg ttttttccag ttaaaaccgg aaccttgcac
     4921 taatagcact ttttcttgtc gcaacaaatc gtagatgaat ttttggtcat ctttaatgcc
     4981 gtacatttcg gtatcaattt tcgggaacat atataacgcc cctttcgctt ttacacagct
     5041 aatacccgga atttgtacca ataattcgtg cattttattt cgttgttcca gcaaacgacc
     5101 gcccggcaac acaaattcat tgatactttg ataaccacct aatgcggttt gaatggcgtg
     5161 ttgcatcggc gtattggcac ataaacgcat agaagagagc atatccaacc cttcaataaa
     5221 acctttggct tgatgcttcg gaccgctcag caccatccag ccctgacgga aaccggcaac
     5281 acgatacgcc ttggataaac cgttataagt tacggttaat acgtccggtg caagagcagc
     5341 aatatggtga tgtaccgcac catcatagac aattttttca taaatttcgt cagcaaaaat
     5401 aattaagtta tgctgacggg caagttctgc aatttccagc aaaatttgac ggctatacac
     5461 tgctccggtc gggttattcg ggttgataac caaaatccct ttagtgcgag gcgtgatttt
     5521 cgatttaata tcttcaagat cagggaacca ctcattttct tcatcgcaca ggtaatgcac
     5581 tgctttacca ccggctaggg ttgaagctgc tgtccataac ggataatcag gcattggaat
     5641 tagaatttca tcgccatcat ttaataacgc ctgcatcgac attgtaatca gttcagatac
     5701 gccgttgccg atgtaaacat cgtttacatc cataccacgc ataccttttg attgataata
     5761 ttgcacaatc gctttgcggg cagaatacaa accttttgaa tcacaatagc cttgtgcggt
     5821 tggaagatta cgaatcacat ccactaaaat ttcatcgggt gcttcaaagc caaaaggggc
     5881 tgggttaccg atatttaatt ttaaaatttt atgcccttct tcttctaagc gaagtgcctc
     5941 tttatgaatt ggcccacgga tatcgtaacg aacctgagct aacttatctg atttgggaaa
     6001 acgatccatt gtaaacttcc tataatgtat tatctaattg tagtctcatc ccgctgttca
     6061 aaaaatcggt aattcggaat gaatttgtaa acattactct aatttgggag gaaaaaaaag
     6121 aggaaatacg ttaaaatgga gacctttttt tcaatcagct aaaaacaagc ggtttctttt
     6181 tctgattttt ttgcaaatgc ttatttttca tcaattaaaa caagaactta caaaaagtta
     6241 ctcaaaagtg ggaagagagc aaggattggt cgccattcaa gcctctatgc cgctattgga
     6301 ggcaaatatt ccgctactcg gttggttgaa aacacaaaaa acaagctacc ctcacttttt
     6361 tctgcaatat cgtgatacag atcagaccat tgccaccatt ggcacacttc gccaatttta
     6421 tacgctagaa gctgcacaat cttttattca acaacatcat ttcccgttaa tcggcgggct
     6481 tcaatttgaa ggcaatacgc aatttatttt gcctcaattt tcattggtaa aaaatcagca
     6541 aaatttaacc gcttgcttct attttgatag tgataacttt gaacaacaag cggttatttt
     6601 tgaacaattt cttgcaaatt tcagccaaat taccgaatta ggactaacgg ataatccgct
     6661 cgtttcaatg agtgccacca gccattttga gcaatggaaa gccaatattg aaaaagcgat
     6721 tgtttctatt gagcagggcg attttcaaaa agtggtattg gcaaatgcga tgagacttca
     6781 ttttgagcaa cccatttgtg cttacagctt gctggcacaa agccaaaaaa caaatttagg
     6841 ttgttaccac tttttatggg cggaaagtgc tgatgttgcc tttcttggct caacccctga
     6901 gcggctatat caacgccaag ggctacactt ttctaccgaa gccctcgccg gcaccgctgc
     6961 ggttaccacc agtgaagtgc aaaccgaact caatgcacaa tggctgttaa gtgatcccaa
     7021 aaatatctat gaaaatcaat tagtggttga tgacattgaa aaccaactca aagattgcgt
     7081 gaccgaattt gcagtatccg aggcaaaaat taagcgtttg cataatgtgc aacacctacg
     7141 ccgccaaatt caagcaacgc tcaaaccgga cacctcagat tcagattgct taagcaaaat
     7201 ccacccaact gcggcagttg cagggcttcc ccgccaaaaa gcaaagggtt ttattaccga
     7261 aaatgagcct tttacccgcc attggtatgc aggtacatta ggcgtaatgg gcaacgagga
     7321 agccgaattt tgcgtaacac tccgttctgc attaatttca caaaattcaa ttacgcttta
     7381 tgccggtgcc ggcattgtgt caggctcaga tccacaatcg gaatggcaag aaattaaacg
     7441 taaggcacaa ggaatggcaa aactgttgga tgttgattta gtaacaaaag gggaactgta
     7501 agctaggctt tcagaactta atctcttaat tatttctttc tatattttca tttccaactt
     7561 cccaacaaaa tatattattg ccaccaaatt agtagtgaat attccataa
//
LOCUS       DANLZC010000119         7602 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.119, whole genome shotgun sequence.
ACCESSION   DANLZC010000119 DANLZC010000000
VERSION     DANLZC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.119"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            229..414
                     /locus_tag="PXE44_002005"
     CDS             229..414
                     /locus_tag="PXE44_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589484.1"
                     /translation="MNKMKQLAPFALLILLLGIVGRMDYDDHIQMQRYKCERNQGFWQ
                     MESNGNQYCGGKYGTKN"
     gene            398..595
                     /locus_tag="PXE44_002006"
     CDS             398..595
                     /locus_tag="PXE44_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589485.1"
                     /translation="MARKIDAGVEIVEVDKRFIAKFFYDGKLQHTTYPQYNRQNAIML
                     INRKIERFNIGRDKPIKLYKE"
     gene            598..1572
                     /gene="ssb"
                     /locus_tag="PXE44_002007"
     CDS             598..1572
                     /gene="ssb"
                     /locus_tag="PXE44_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5589486.1"
                     /translation="MAGINKVIIVGRLGNDPELRTMPNGDSVAKISVATSTEWTDKTS
                     GEKKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIA
                     DQLQMLGSSNSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIRVRWITKEQA
                     KQFINNISTDWMKTICSFALLTGARRTEILSMTWDKIDFIRKVAIVSNDIAKSEKARS
                     LLLNDDAIELLQKQRGKNPKYVFVGSKGQALQDINRKIFNKATEKCLLVDFHFHDLRH
                     TWASWHVQAGTPLFTLKELGGWETLEMVKKYAHLNADHLLNHANSVKF"
     gene            complement(1659..1735)
                     /locus_tag="PXE44_002008"
     tRNA            complement(1659..1735)
                     /locus_tag="PXE44_002008"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1699..1701),aa:Arg,seq:tct)
     gene            complement(1760..1836)
                     /locus_tag="PXE44_002009"
     tRNA            complement(1760..1836)
                     /locus_tag="PXE44_002009"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1800..1802),aa:Pro,seq:tgg)
     gene            2006..2866
                     /gene="folD"
                     /locus_tag="PXE44_002010"
     CDS             2006..2866
                     /gene="folD"
                     /locus_tag="PXE44_002010"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDL5589487.1"
                     /translation="MTAQIISGSEVSSQIKKKVSEKIAHYLEQGKRNPGLAVILVGND
                     PASQVYVGSKRKSCAEIGIASKSYDLPEETTEAELLALIEKLNQDDSVDGILVQLPLP
                     KQIDSTKVIEAILPHKDVDGFHPYNVGRLSQRIPTLRPCTPYGVMKLLETTGVDLYGQ
                     HAVIVGASNIVGRPMALELLLGGCTVTVTHRFTKDLEHHVRQADILVVAVGKPEFIPG
                     EWVKDGAIVIDVGINRGADGKLKGDVEYSVASQKAAFITPVPGGVGPMTVAMLMQNTL
                     QTYENHLQAV"
     gene            2980..4431
                     /gene="thiI"
                     /locus_tag="PXE44_002011"
     CDS             2980..4431
                     /gene="thiI"
                     /locus_tag="PXE44_002011"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL5589488.1"
                     /translation="MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNVLLKETENVSVV
                     RHWDFIEVRAKVDEETALVLDLLKRTPGIHHILEVEETPFTDMHDIFEQTYAAVKAEL
                     EGKTFSVRVRRKGKHDFRSLDVERYVGGGLNQRIETAKVKLTNPDVTVRIDIEHDKML
                     LIKARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEI
                     GVKQMAYHIWSRYSTSHKVRFIAINFENVVGEILEKVDNGQMGVVLKRMMVRAASRIA
                     ERFDIQAIVTGEALGQVSSQTLTNLRLIDKASDTLVLRPLITHDKEQIIAMAKAIGTD
                     DIAKSMPEFCGVISKNPTVKAIETKILEEEGNFNFEVLEQAVQTASYLDIREIAQQTE
                     KEVVSVETTTELSKNDVVLDIRSPEEVDEKPFRLDGVEVKELPFYKLSSQFSTLDQSK
                     TYLLYCQRGVMSKLQALYLKENGYSNVKVFRVK"
     gene            complement(4476..5183)
                     /locus_tag="PXE44_002012"
     CDS             complement(4476..5183)
                     /locus_tag="PXE44_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HDL5589489.1"
                     /translation="MFKEKPVISNQHGALAMAMLPFLYAVIESGFSTLHIFFGLSWLF
                     LYFFSYPFLSLFSKKPTERNKKWAKIYFGLSILFALPLIYAQPSILQFLPIILPLGLV
                     QVHYAKQKDERNLWNDIAGILTFGIMGMAAYYLVTKQYQFEILLHPTLFFIATTFYIK
                     SMVRERRNPIYMEISIAIHLVLAMLYILCSLQAVFFAYLIATARAIIVPSFGWNVKQV
                     GMLEFPVILLFLASLIW"
     gene            complement(5251..6225)
                     /gene="pfkA"
                     /locus_tag="PXE44_002013"
     CDS             complement(5251..6225)
                     /gene="pfkA"
                     /locus_tag="PXE44_002013"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL5589490.1"
                     /translation="MSKKIKKIAVLTSGGDAPGMNAAIRGVVRTALNEGLEVCGIQDG
                     YYGLYHDKVIPLDRRSVSETINRGGTFLGSARFPDFKRPEVRKHCVETLKKYSIDALV
                     VIGGDGSYMGAKLLTEEFGYPCIGIPGTIDNDIVGTDYTIGYQTALETALDAIDRLRD
                     TSSSHQRISIVEIMGRHCGDLTLSAALAGGCEYVIVPEKGLDKDALMRSIEEGFHKGK
                     RHSIIAITELMTDVHALAKEIEARFGNETRATVLGHIQRGGSPCAFDRILASRMGVYA
                     VELLLQGFGGHCVGIQNEQLVHHDIIDAINNMRRPFKEELFEASRKLF"
     gene            complement(6332..7528)
                     /locus_tag="PXE44_002014"
     CDS             complement(6332..7528)
                     /locus_tag="PXE44_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL5589491.1"
                     /translation="MLNTQFRTHFPFFEKSDGWTYLDSAATTLKPDVLIEATSEFYAS
                     AGSVHRSQYDLPQTEQYELARDLVAKRFNVETREAVIWTSGTTQSINTVAYGLEHQIA
                     AGDEIVISVAEHHANFIPWQQLAQRTRAKLVVLPLNDKYEIELETLKNALSEKTKIVA
                     FNLVSNVTGVRQPAEQIIPLIRQSCSAKIVLDIAQAVCSEQVDVQKLDADFYAFSAHK
                     MYGPNGVGVLTGKLQSLEQIQPLVFGGKMLKNITESELTVAELPYRLEAGTPNIAGII
                     GFGQVLAWLEKWDFVALNHQLYDLAEYTCKRLNSYQNLQIIGQQNTPTISFVVKNQHP
                     ADIAAFLTQSKIAIRQGEHCAKPYLRHLEEVGTVRLSLAHYNNQQDVEHFFNALDFTL
                     EILQES"
BASE COUNT         2347 a         1491 c         1663 g         2101 t
ORIGIN      
        1 ctggtatcag tcctaagcaa tcacgcttat ctcaagtttg agtaagtacg gatacaagca
       61 gcacccgacg gaatggcgaa agccgggcgg gcgacggcgt gggcagacac gcatttattc
      121 caaagctcat ttcaccaagt gtgtttcgga atacccacaa ctagacgcaa gtcgctccat
      181 ttgcccaccg taaaacgtgg gcttttttta accctaaagg acaatcctat gaacaaaatg
      241 aaacaacttg ccccatttgc cttgctaatt ttgctattag gcattgtcgg gcgaatggat
      301 tatgacgacc acatacaaat gcaacgttac aaatgcgaac gaaatcaagg cttttggcaa
      361 atggaaagca acggcaacca atactgcgga gggaaatatg gcacgaaaaa ttgatgccgg
      421 tgttgagatt gttgaggtag ataaacgatt tatcgccaag tttttttatg acggcaaatt
      481 acagcacaca acctatccac aatacaaccg ccaaaacgca attatgttga ttaaccgcaa
      541 aattgagcgg ttcaacattg gcagagacaa accaatcaaa ctttataagg aataacaatg
      601 gcaggaatta acaaagtgat tattgtcggt cgcctaggta acgacccgga actacgaaca
      661 atgccaaatg gcgattcagt cgccaaaatc agtgtagcaa cttcaaccga atggacggat
      721 aaaacctcag gcgagaaaaa acaagccaca gagtggcata gcattatcgc ctttcggaat
      781 ttagctgaca ttatcggcaa atacctgaaa aaaggctcgc aagtgtatgt ggaaggcaag
      841 ctacgcactc gcaaatggca agcccaagac ggcacagacc gctggacgac cgaaatcatc
      901 gccgatcagc tacaaatgct cggtagctca aacagcggaa acagcaataa taattgggca
      961 accgaaccgg caggaaaccc accaccaacc aatccatata accacgtaat gacggattca
     1021 gaatcacgag attttgatga tgatatccgg gttcgttgga ttaccaaaga acaagccaag
     1081 cagtttatta ataatattag tactgattgg atgaaaacta tctgctcttt tgctttatta
     1141 acaggagcaa ggcgaacaga aatactttct atgacttggg ataaaataga ttttatccgt
     1201 aaagtagcga ttgtttcgaa tgatattgcc aaatctgaaa aagcgagatc gcttttactt
     1261 aatgatgatg caattgaatt attacaaaag cagagaggga aaaatcctaa atatgttttt
     1321 gtaggaagca aagggcaggc attacaagat attaaccgca aaatatttaa caaagcgact
     1381 gaaaaatgtt tattggttga tttccatttc catgatttaa ggcatacttg ggccagttgg
     1441 cacgttcagg ccggcacacc attatttacc ttaaaagaat tgggtggctg ggaaacatta
     1501 gagatggtaa agaaatatgc ccatcttaat gcagaccatt tactcaacca cgctaactca
     1561 gttaaattct agtcacaaat tagtcacaaa gtatttttga cataggaaat taataactga
     1621 tatttaaatg taactatttg aaatataagg gtaattaatg gcacgcccta aaggattcga
     1681 acctttgacc cacgccttag aagggcgttg ctctatccag ctgagctaag ggcgcagatg
     1741 aagataatat aaaaagaagt ggtcggcgag ataggatttg aacctacgac ccactggtcc
     1801 caaaccagtt gcgctaccaa gctgcgctac tcgccgacat aagatgttgt tgattataga
     1861 tttctttgca caacagtcaa tctttttttg tgaatgtcgg cttaaatgat tgaagtttat
     1921 ccgttattca aaattagctt atcaaaaagg ctattttttg agagaatgac gaatgttttg
     1981 ccaagtgata tttcgggagg tagtaatgac tgcacaaatt atttcgggga gtgaggtttc
     2041 ttcacaaatt aaaaaaaagg tgagtgagaa aatcgctcat tatcttgaac agggtaaacg
     2101 taaccctggt cttgcggtca ttttagtggg gaatgaccca gcctctcaag tgtatgtggg
     2161 gagtaaacgc aaaagctgtg ccgaaattgg gattgcgtcg aaatcttatg atttacccga
     2221 agaaaccaca gaggctgaat tattagcact gattgaaaag ttgaatcaag atgattcggt
     2281 cgatgggatt ttagtccaat taccgttacc taaacagatc gattcgacta aagtgattga
     2341 agccatctta ccacataaag atgttgatgg attccaccct tataatgttg ggcgtttatc
     2401 tcagcgtatt cccactttac gcccttgcac gccatacggt gtgatgaagt tgcttgaaac
     2461 aactggggta gatttatatg gtcaacacgc agtgattgta ggtgcgtcta atattgttgg
     2521 tcgcccgatg gcgttagaat tgttgcttgg tggttgtaca gtcacagtta ctcatcgctt
     2581 taccaaagat ttagagcatc acgttcgcca agcagatatt ttagttgtgg cagtcggcaa
     2641 gcctgaattt attcccgggg agtgggttaa agacggtgct atcgtgattg atgtagggat
     2701 taaccgtgga gcagacggca aactaaaagg tgatgtagag tattcagttg cctcacaaaa
     2761 agcggcattt atcacccctg tccccggtgg ggtaggcccg atgacggtgg caatgttaat
     2821 gcaaaatacc ttgcagacgt atgaaaacca tttacaagcg gtctaatttt acggatattt
     2881 tgcaaatcct cggcattggt cgaggatttc tttttctggg ctttttcagc agaaaaggct
     2941 aaaatacttg ccatttttat tacacgatcc ttattaaata tgaaatttat catcaaactt
     3001 ttccctgaaa ttatgattaa aagcgactct gttcggaaac gctttatcaa aattttaacc
     3061 tcaaatattc gcaacgtatt attaaaagag actgaaaatg tgtctgtggt tcgccattgg
     3121 gactttattg aagttcgggc gaaagtggac gaagaaacgg cattagtgct ggatttgctt
     3181 aaacgcacgc cgggtattca ccatatttta gaagtggagg aaaccccttt tacggatatg
     3241 cacgatattt ttgagcagac ttatgccgca gtgaaagctg aactagaagg caaaacgttt
     3301 agcgtgcggg ttcgccgtaa aggaaagcac gatttccgtt ctcttgatgt agagcgttat
     3361 gtcggtggtg ggctgaatca gcggattgaa acggcgaaag tgaaattaac caacccagat
     3421 gttacggtca gaattgatat tgaacatgat aaaatgttgc tgattaaagc tcgccacgaa
     3481 gggttaggcg ggtatccaat cggcacacaa gaagatgtgt tgtcattgat ttcgggtggt
     3541 tttgattcgg gtgtatcgag ctatatgctg attcgcagag gttctcgtgt gcattactgt
     3601 tttttcaatt taggtggtgc cgcacacgaa attggcgtga aacagatggc atatcatatt
     3661 tggagccgtt atagtacctc gcacaaagtc cgttttatcg ccattaattt tgaaaatgta
     3721 gtgggtgaga ttttagaaaa agttgataac ggacaaatgg gcgtggtatt aaaacgtatg
     3781 atggtgcgag cggcaagccg tattgccgag cgttttgata ttcaagcgat tgtgaccggt
     3841 gaggcgttgg ggcaggtctc tagccaaaca ctgactaatt tacgtttaat tgataaggca
     3901 tcagatacgc tggtattacg tccgctcatc actcacgata aagagcagat tattgcaatg
     3961 gcaaaagcga taggcactga cgatattgct aaatcaatgc cggagttctg tggagtaatt
     4021 tctaaaaatc cgacggttaa ggcaatcgaa actaaaattt tggaagaaga aggcaacttt
     4081 aattttgagg tactagaaca agcggttcaa acggcgagtt atttagatat tcgtgagatc
     4141 gctcagcaaa ccgaaaaaga ggttgtttcg gttgaaacca caacagaatt gagtaaaaac
     4201 gatgttgtgc tagatattcg cagcccggaa gaggttgatg aaaaaccatt ccgtttagac
     4261 ggggttgaag taaaagaact cccgttttat aaactttcca gccagtttag tacgcttgac
     4321 caaagcaaaa cctacttgct ctattgccaa cgtggggtaa tgagtaaact gcaggctctt
     4381 tatttgaaag agaatggcta cagcaatgta aaagtgtttc gagtgaaata atgcctcttt
     4441 gattgcacct taatgattga ttaaggtgca attttttacc aaataaggct tgctaaaaag
     4501 agtaagatca caggaaattc aagcattcca acttgtttaa cattccagcc aaagcttggc
     4561 acaataatgg ctcgggcagt tgcaattaaa taagcaaaaa agaccgcttg tagagagcag
     4621 agtatgtaaa gcattgctaa aaccaaatgt attgcaatac tgatttccat ataaattggg
     4681 tttcttcgct ctcgcaccat actctttata tagaaagtcg tagcaataaa aaatagggta
     4741 gggtggagca gaatctcaaa ctgatattgt ttcgttacca gataataagc tgccattccc
     4801 attatgccaa aggttagaat gccggcaata tcattccata aattgcgttc atctttttgt
     4861 ttggcgtagt gaacttgcac taacccaagc ggtagaatta tcggtaaaaa ttgcaaaatc
     4921 gaaggctgag cgtagatcaa aggtaatgca aatagaatgc ttaagccaaa gtagattttc
     4981 gcccattttt tattgcgttc agttggcttt ttactgaaaa gtgagagaaa agggtaggaa
     5041 aagaagtaga gaaacagcca cgacagccca aagaaaatat gcaaagtgct aaaaccactt
     5101 tcaataacgg cgtagagaaa cggcaacatt gccattgcta atgcaccgtg ttggttggat
     5161 ataacgggtt tttctttgaa cattcaattt ccctcttaat gtggcaaata aaaaggcgac
     5221 taaacagccg cctttaaatt gtaagaaaag ttagaataat tttctagagg cttcaaataa
     5281 ttcttctttg aacggacgac gcatattatt gatcgcatca ataatatcgt ggtgaaccaa
     5341 ctgctcgttt tgaataccga cacagtgacc gccaaagcct tgcagtaata attccaccgc
     5401 atagacgccc atacgggagg ccaaaatacg gtcgaaggca caaggcgaac caccacgttg
     5461 aatatgacct aatacggttg cacgggtttc attgccgaaa cgtgcttcaa tttctttcgc
     5521 taatgcgtga acatcggtca tcaattctgt aattgcaata attgaatggc gtttaccttt
     5581 gtggaaacct tcttcaatgc ttcgcattaa tgcatcttta tctaagcctt tttccggtac
     5641 gatcacatat tcgcaaccac cggcgagggc agcacttagg gttaaatcac cacagtggcg
     5701 acccataatt tccacaatcg aaatacgttg gtgtgaactt gaagtatcac gcaggcggtc
     5761 aatcgcatct aatgcggttt caagggcagt ttgatagccg atggtgtaat ccgttcccac
     5821 aatatcgtta tcaatcgtac ccggaatacc gatacaaggg taaccaaatt cttcggttaa
     5881 taatttagcc cccatataag aaccgtcgcc accaataacc actaatgcat caatgctgta
     5941 tttttttaag gtttcgacac aatgtttgcg aacttctggt cttttaaaat cagggaagcg
     6001 ggcagaacct aagaatgtac cgcctcggtt gatggtttca gacacggaac ggcggtcaag
     6061 tgggatcact ttatcgtggt aaagcccata gtagccgtct tgaataccac aaacctctaa
     6121 accttcatta agtgcggtac gaaccacacc acggattgcc gcgttcattc ctggtgcatc
     6181 accaccactg gttaatactg caattttttt gattttttta ctcatttaaa aacaccttgt
     6241 tgataaatta aagttaaatt ggaaactggt gcaaggttac aatattcagc cttttcgttc
     6301 tactaaaaaa tacgtatttt tttgatttag cttagctttc ttgcaaaatt tcaagggtaa
     6361 aatctagagc gttgaaaaaa tgctcaacat cttgttgatt attgtaatgg gcaagtgaaa
     6421 gtcgaactgt tccgacctct tctaaatggc gtaaataagg cttggcacaa tgctcccctt
     6481 ggcggatcgc aattttactt tgggttaaga aagcggcaat atcggcaggg tgctgatttt
     6541 tcaccacaaa gctaatagtc ggtgtatttt gttgcccgat aatttgcaaa ttttgataag
     6601 aatttaaccg cttgcaggtg tattcagcca agtcataaag ctgatgatta agagcgacaa
     6661 aatcccattt ttcgagccaa gctagcactt gcccaaagcc gataatgccg gcgatattcg
     6721 gcgtgccagc ttccaagcga taaggtaact ctgccacagt taattcactt tccgtaatgt
     6781 ttttcagcat tttgccgcca aatacaagcg gttgaatttg ctccaaactt tgcaatttgc
     6841 cggtaagcac gcccacaccg tttggaccat acattttatg ggcggaaaaa gcgtagaaat
     6901 ctgcatctaa tttctgaacg tccacttgct ctgagcaaac tgcttgagca atatctaaca
     6961 caattttagc agagcaagat tggcggataa gcgggattat ttgttccgct ggctggcgaa
     7021 ctcctgttac gttagaaact aaattaaagg caacaatttt ggttttttcc gataacgcat
     7081 ttttaagggt ttcaagctca atttcatact tatcattaag tggcaacacc acgagtttcg
     7141 cccttgtacg ctgtgccaac tgctgccaag gaataaaatt agcgtgatgt tccgccactg
     7201 aaattacaat ttcatcgcct gctgcaattt gatgctccaa gccataagcc acggtgttaa
     7261 tactttgggt tgtaccgctt gtccaaatca ccgcttcacg agtttccaca ttgaaacgtt
     7321 ttgcgactaa atctcgagct aattcatatt gttcggtttg tggcaaatcg tactgactgc
     7381 gatgcaccga ccccgcagaa gcgtaaaact cacttgttgc ttcaatgagt acatcaggtt
     7441 tcagcgttgt agcggcagaa tctaaatacg tccatccgtc agatttttca aaaaaaggga
     7501 aatgggtgcg aaattgggtg ttaagcataa gttaattcct aagagttgtg gaaaattgag
     7561 ttaccaagaa aatacctatt ataaaggaat gttttgaggt aa
//
LOCUS       DANLZC010000120         7589 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.120, whole genome shotgun sequence.
ACCESSION   DANLZC010000120 DANLZC010000000
VERSION     DANLZC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.120"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            125..2272
                     /locus_tag="PXE44_002015"
     CDS             125..2272
                     /locus_tag="PXE44_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5589492.1"
                     /translation="MKRLRYSYLALILGSALPLSAYADGYLSNDNIRQFDARFNFFLA
                     NPLPAPIAKADGRGEAIENLVVKYASDYLNDIYLKEDVIRFRRWQWQDLYTSLDLKTK
                     QKPDYLNTYRLQAEAYFINKQYQEALSQLDQILRRNPNDVHAMAMSVVASRVLENPNT
                     EAERLLALYQISPSIAQKVQQYLHFTDTMLAASYGSEPQTTMIPDTIAVFGQSPNPDG
                     TPSKGLLQRLEKTKEMAEKFPNAKIILSGGPVRYIYAEADVMKKWLIENGVDESRLLL
                     DDVARDTPGNAVGIIDFMKEYGGRKVLGIGTILHLPRAMSVLKSYADSIGYELEIDSA
                     GGGSPPNEKNKKGEALYTFVNVARAMGLFTLGDFENLLAKEQAEKEQREALEKAQQNY
                     LEKLVAERDQAVKNAENSANELEVARQALETAKQAHQQAEEELNKELSKTQEQLKQST
                     EALSRLIIKQNNPFSEHYVVQLNLMEQATYDLYRRLAEIEHTQDWNLWLSDSLSKYRL
                     KNRSTEDYHLLHIGLDHQVSHNWSIGGVFGISQGQIENRTHKVKGTSAGIYAGYRPTS
                     GLFWTNFFNYSHFTNQSGVTKRDNNAYSLSSEIGRNIVMSHQWQITPKLQLGWAKAGS
                     ARMFWAGTGLRLGKVIELNAIKLKPYADISYRFRNRHIDTLDSLSDRHRNVISIGIQA
                     VGNRHYFAIEGSMLHGDALKRSYGLHANYHYQW"
     gene            2285..3403
                     /locus_tag="PXE44_002016"
     CDS             2285..3403
                     /locus_tag="PXE44_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5589493.1"
                     /translation="MKKIKTTLSALALSLSLISVNSWAIPADYLSQDNISQYDKAFNF
                     VLAKVLPAPKGKEDGRGAPIEHLVIQYAPDYVDDPYLKEDVLNFRRWYWQAMYASLDQ
                     KVKEKPTYLNTYRLQAEAYFVNKEYREALSQLDQILRQNPRDIHALAMTVLASRVLED
                     TEQETARLSALKQLAPEVAQRMTDFFAFTEQTLAASYGNEPQTAMIPDTIAVFGSTPN
                     KDGTPSPLLLDRLQKTKEMAQKFPQAKIIVSGGAVKTEFAEADVMAKWLSENGIEKDR
                     LLLDPVARDTPGNAVGIIKLMQTYNGKKVLGIGTLQHIPRATAVLKGYAQHIGYPIEM
                     DSVGGGKPATPGKAKIEALYTYVNVARSMGLFELGDFK"
     gene            complement(3464..4726)
                     /gene="rho"
                     /locus_tag="PXE44_002017"
     CDS             complement(3464..4726)
                     /gene="rho"
                     /locus_tag="PXE44_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL5589494.1"
                     /translation="MHLTQLKNTPVSKLVEMGENQMGLENLARLRKQDIVFAILKQHA
                     KSGEDIFGQGVLEILPDGFGFLRSSDSSYLAGPDDIYVSPSQIRRFNLQTGDKIEGKI
                     RPPKEGERYFALLKVDSVNDDKPEASRSKILFENLTPLHANSRLKMERGNGSKEDLTA
                     RILDLAAPIGKGQRALIVAPPKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVT
                     EMQRTVRGEVIASTFDEPATRHVQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYN
                     TVTPVSGKILSGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVDTGSKMDEVIFE
                     EFKGTGNMELHLSRRIAERRIFPAIDFNRSGTRKDDLLMAADELQKTWMLRKVLNPMD
                     EVDAIEWLIDKLMMAKTNEEFFEIMKRS"
     gene            complement(4912..5859)
                     /gene="cysK"
                     /locus_tag="PXE44_002018"
     CDS             complement(4912..5859)
                     /gene="cysK"
                     /locus_tag="PXE44_002018"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL5589495.1"
                     /translation="MTIYADNSYSIGNTPLVRLKNFGNGNIVVKVEGRNPSFSVKCRI
                     GANMVWQAEKDGILTKDKEIVDATSGNTGIALAYVAAARGYKLTLTMPETMSTERKRL
                     LRGLGVNLVLTEGAKGMKGAIAKAEEIAASDPDRYVTLKQFENPANPAIHQQTTGPEI
                     WDATEGKIDVLVAGVGTGGTITGVSRAIKQDKGKQIISVAVEPSESPVITQTLNGEEV
                     KPGPHKIQGIGAGFIPKNLDLSLLDRVEQVSSEEAITTARRLMAEEGILGGISSGAAV
                     AAADRIAKLPEFADKLIVVILPSASERYLSTLLFEGIEA"
     gene            complement(6033..7529)
                     /gene="pepA"
                     /locus_tag="PXE44_002019"
     CDS             complement(6033..7529)
                     /gene="pepA"
                     /locus_tag="PXE44_002019"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDL5589496.1"
                     /translation="MEFSVKNGSVEKQRTACLVVGVYEPRRLCSAAEQLDKLSNGYIS
                     TLLRRGDLEGKAGQTLLLHNVPNVLADRVLLVGCGKERELTERQYKQIIQKSVQTINE
                     TGSMEAVSFLTELHVKGRTPYWNVRFAVEAIQEQLYAYNDFKSIKPEVRRELRRVIFN
                     VAHRKDLPDAERALEHGKAVALGISHAKNVANCPPNVCNPHYLAELAKGLAYDYPAIS
                     TTIVDEKQMAELGMNAYLAVSRASENEAFLSVIEYKGNPNADAKPIILVGKGMTFDSG
                     GLSIKPAEAMDEMKYDMCGAATVYGTIKAIAEMKLPLNVIGLLAGCENAVDGKAYRPG
                     DILTTMSGLTVEVLNTDAEGRLVLCDALSYVERFEPELVIDIATLTGACMIALGNHNS
                     GLMSPHNGLANELLAAAEQADDKAWRLPLGEEYQEQLKSNFADLANIGGRLGGACTAG
                     QFLSNFTKKYTWAHLDIAGTAWKSGAAKGATGRPVSLLTQFLINKAQV"
BASE COUNT         2194 a         1519 c         1629 g         2247 t
ORIGIN      
        1 aattaaaaat gattttgaat attattaata aaatagcaca aacaaatttt atccttaatc
       61 aactttgttg gttagatgta aaaagttata aatacttctt cacccaatat aaggagaaat
      121 cataatgaaa cgcttgcgtt attcttatct tgctttgata ttaggttcag ccttaccttt
      181 atcggcttat gctgacggtt atttaagtaa cgataatatt cggcaatttg atgcccgttt
      241 taattttttc ctcgcaaatc cactacctgc gcctattgca aaagcagatg ggcgtggtga
      301 agcgattgag aatttggtgg tgaaatatgc atcagattat cttaatgata tctatcttaa
      361 agaggatgtt attcgttttc gccgttggca gtggcaagat ctctatacgt cattggattt
      421 aaaaacgaaa caaaagcccg attatttaaa tacttaccga ttacaggcgg aagcgtactt
      481 tatcaataag caataccaag aagcattatc tcaactggat caaattctgc ggcgtaaccc
      541 taatgatgtt catgcaatgg caatgagtgt ggttgcaagt cgggtattgg aaaatcctaa
      601 tactgaagcc gagcgtttat tagcgcttta tcaaatctct ccaagtattg cgcaaaaggt
      661 gcagcaatat ctacatttta cagacactat gcttgctgcc agctatggta gtgagcctca
      721 aacaacaatg atccccgaca ccatagcggt atttgggcaa tcgcctaacc cagacggtac
      781 tccaagcaag ggattattgc aacgtcttga gaaaaccaaa gaaatggcag agaaatttcc
      841 gaatgcaaaa attattttaa gtggtggacc tgttcgttat atctatgcag aagcggatgt
      901 aatgaaaaaa tggttgatag agaatggcgt tgatgaaagc agactgttgc ttgatgatgt
      961 ggcacgagat actccgggaa atgctgtcgg tatcattgat tttatgaagg aatatggagg
     1021 tagaaaagta ttggggatcg gtacgatctt acatttaccg agagcaatgt ctgtattgaa
     1081 aagctatgcg gattccattg gatatgaatt ggaaattgat tctgccggag gcggttctcc
     1141 tcctaatgaa aaaaataaaa aaggtgaagc tctttataca tttgttaatg ttgctcgtgc
     1201 aatggggtta tttaccttgg gcgattttga gaatttgctt gccaaagaac aagctgaaaa
     1261 agaacaaaga gaagcgttgg aaaaagcaca gcagaattat ttagaaaaat tggtagctga
     1321 gcgagatcaa gcagtaaaaa atgcagaaaa ttctgcaaat gagctggaag tagccagaca
     1381 ggcgttagaa acagcaaaac aggctcatca gcaagctgaa gaggaattga ataaagaact
     1441 tagtaaaacg caagagcagt taaaacagag tacggaagcc cttagtcgtt taataataaa
     1501 gcagaacaat cctttttccg agcattatgt tgtacagctt aatttgatgg aacaagcaac
     1561 ttatgatctg taccgtaggt tagcagaaat agaacataca caagattgga atttatggct
     1621 ttcggatagt ctatccaaat atcgtttaaa aaatcgttca accgaagact atcacttatt
     1681 acatatcggg ctagatcatc aggtttccca taattggtct attggtggag tatttggtat
     1741 cagtcagggg caaattgaga ataggacgca taaagtgaaa gggacatctg ctggtattta
     1801 tgctggttat cgtcctacaa gtggtctgtt ttggacaaat ttttttaatt acagtcattt
     1861 tactaatcaa tctggtgtaa caaaacgaga taataatgca tacagtctga gtagtgaaat
     1921 tggaagaaat atcgtgatga gtcatcaatg gcaaattacg ccaaagctgc aattaggctg
     1981 ggcaaaagct ggatcagcac gaatgttttg ggctgggaca gggctacgtt taggaaaagt
     2041 gattgaactc aatgcaatca aacttaaacc ttatgctgat attagctatc gttttcgaaa
     2101 tcgtcatatt gataccttag atagtttatc tgatcgtcat cggaatgtaa tcagtattgg
     2161 gatacaagca gttggaaatc gccattattt tgcaatcgaa ggatcaatgt tacacgggga
     2221 tgcattgaag cgttcatacg gattacacgc aaattatcac tatcaatggt aaataggagc
     2281 ctctatgaaa aaaatcaaaa caaccctttc tgcccttgct ttaagccttt ctttgataag
     2341 tgtaaatagc tgggcgatac cagctgatta tttgagccaa gacaatatca gtcaatatga
     2401 taaggcgttt aactttgtat tagcaaaagt actgcctgca ccaaaaggta aagaagatgg
     2461 tcgaggagcg ccgattgagc atttagttat tcaatatgcg ccagactatg ttgatgaccc
     2521 ttatctaaaa gaggatgtgc tgaatttccg ccgttggtat tggcaagcga tgtatgcttc
     2581 gcttgatcag aaagttaaag agaagccgac ctaccttaac acttaccgtt tacaagcaga
     2641 ggcgtatttc gtcaataaag agtatcggga agcactttct cagctcgacc aaattttgcg
     2701 ccaaaacccc cgtgatattc acgctcttgc gatgacagta ttagcttcaa gggtgctaga
     2761 agatactgaa caagaaacgg ctcgtctttc tgcactgaaa cagcttgctc ccgaagtcgc
     2821 acaacgaatg acagactttt ttgcctttac cgaacaaacg ctggcggcaa gctacggtaa
     2881 tgaaccacag acagcaatga tccctgatac gattgccgtt tttggttcta cgccaaacaa
     2941 agacggcaca ccttcgccgc tgttgttaga tcgattacag aaaaccaaag agatggcaca
     3001 aaaatttccg caagcaaaaa ttatcgtcag tggcggtgct gtgaaaacgg aatttgctga
     3061 ggctgatgtg atggcaaaat ggcttagcga aaatggtatt gaaaaagacc gtttgctact
     3121 tgatcctgtt gccagagata ccccgggaaa tgcggttggg attatcaagc tgatgcaaac
     3181 ttataatggc aagaaagtat taggtatcgg tacgttacaa catatccctc gtgcaacagc
     3241 ggttctcaaa ggttatgcac agcacattgg ttatccgatt gagatggatt ccgttggtgg
     3301 tggcaagccg gcaacaccgg gaaaagccaa aatcgaggca ctttatactt atgtaaatgt
     3361 ggcgagatca atggggttgt ttgagttggg ggattttaag tagttacttt attgacagct
     3421 gctattgata aaaatcccgc gttaagcggg atttttttat gttctacgaa cgtttcataa
     3481 tctcgaaaaa ctcttcgtta gtttttgcca tcattaattt gtcgattagc cattcgatag
     3541 catctacttc gtccattggg ttgaggactt tgcgtaacat ccaagttttt tggagttcgt
     3601 ctgctgccat taagaggtca tctttacgag tacctgagcg gttgaagtca atagccggga
     3661 agatacggcg ttcagcaata cggcgtgaga ggtgtaattc catattaccc gtacctttga
     3721 actcctcgaa aatcacttcg tccatttttg atccggtatc cacaagtgcg gttgcgataa
     3781 tggttaagct gccaccttct tccacattac gggctgcacc gaagaaacgt tttggacgat
     3841 gtaaggcgtt agcgtccaca ccaccggaaa ggattttgcc ggaaaccggg gtaacggtgt
     3901 tgtaggcacg ggctaaacga gtgatggagt ctaataaaat caccacatct tttttatgct
     3961 ctaccgagcg ttttgctttc tcgattacca tctccgcaac ttgaacgtgg cgggttgccg
     4021 gttcatcaaa ggttgaggca attacttcac cacgtacggt gcgttgcatc tctgttactt
     4081 cttccggacg ctcatcaatt aataatacga taagctcaca ttcaggatga ttatgggtga
     4141 tgctttgtgc aatattttgc agtaacacgg ttttaccagc ttttggcggg gcaacaatta
     4201 acgctcgctg acctttaccg attggggcgg ctaaatctaa aatacgagcg gttaaatctt
     4261 cttttgatcc attgccacgc tccattttta agcgagaatt ggcgtgcaga ggggttaagt
     4321 tttcaaagag aattttactg cgagaggctt ccggtttgtc atcattaact gaatcaactt
     4381 tcagtagggc aaaatagcgt tctccctctt ttggtgggcg aattttacct tctattttat
     4441 cgccagtttg taagttaaaa cggcggattt ggcttggaga aacataaata tcatcagggc
     4501 cggctaaata tgagctatct gatgagcgga ggaaaccgaa gccgtccggt agaatttcaa
     4561 gcacgccttg cccgaaaatg tcttcaccgc tttttgcgtg ttgtttaaga atagcaaata
     4621 cgatgtcttg tttacgtaaa cgagccagat tttctaagcc catttgattt tcacccattt
     4681 ccacaagctt tgaaacaggg gtgtttttaa gttgagtaag atgcataatt tgaatgtaaa
     4741 attgtgatta attaagattg gatttgttga tattgattag atacggccaa ccgtaaagat
     4801 gtggcgaaga ataacgcaat aggagacaaa ttgcaaattt tttataaaaa ataaccgctt
     4861 gtttgcaata aaaatgccga catcagccgg catttttata ttaatttaat attacgcttc
     4921 aatcccttca aacagtagcg tacttaaata acgctctgag gctgaaggca gaatcactac
     4981 aattaattta tcggcaaatt caggtagttt agcgatgcga tctgcggcag caacggcagc
     5041 acctgatgaa ataccgccta aaatgccttc ttctgccatt aaacggcgtg cggttgtaat
     5101 cgcttcttcg cttgaaactt gttcaacacg gtcaagcagt gataaatcta agtttttcgg
     5161 aataaagcct gcaccaatgc cttgaatttt gtgcgggccc ggtttgactt cttcaccgtt
     5221 taaagtttgc gtaatgaccg gcgattcact cggttctacc gccactgaaa taatttgctt
     5281 gcctttatct tgcttaatag cacgggaaac accggtaata gtgccacctg ttcctacgcc
     5341 tgccactaat acatcaattt taccttcagt tgcatcccaa atttcaggac cggtagtttg
     5401 ttggtgaatt gccgggtttg ccgggttttc aaactgtttt aaggtaacat agcgatctgg
     5461 gtcggaagcc gcgatttctt cagctttcgc aatcgctcct ttcattcctt ttgccccttc
     5521 ggttaagact aaatttactc ctaagccacg caataagcgt ttacgctcgg tactcattgt
     5581 ttccggcatt gtgagcgtta atttataacc acgagcggct gctacataag ccaaggcaat
     5641 tccagtgtta ccgctggttg catctacaat ttctttgtct ttggttagaa taccgtcttt
     5701 ctctgcttgc cacaccatat tcgcaccaat acggcatttc acgctaaaac ttgggttacg
     5761 cccttccact tttaccacaa tattgccgtt gccaaaattc tttaaacgca ccagcggggt
     5821 attgccgatt gagtatgagt tgtctgcata gatagtcata gtgatattcc ttaataagta
     5881 ttgagattgg gatttatcat acttattttt aatcgctttt ctagtagctg gaatttattt
     5941 gatataacta aaaagaatat aggggcgtat gaagaggatc ggtctgattt tctttttgca
     6001 aaaactcgat aaaatcagac cgcttgtgag agttagacct gggctttatt gatcaaaaat
     6061 tgtgttagta aggataccgg tctgccggtc gcccctttgg ctgccccaga tttccaagcc
     6121 gtgccggcaa tatctagatg cgcccaagta tatttctttg taaagttaga gaggaattga
     6181 ccggcagtac aggctccgcc taaacgtccg ccgatattag caagatcagc aaagttagat
     6241 tttaactgtt cttgatattc ttcccctaaa ggtaaacgcc acgctttgtc atcggcttgc
     6301 tctgcggcag ctaagagttc gtttgctaaa ccgttatgag gtgacatcag cccgctgtta
     6361 tgattaccta aggcaatcat gcaagcacca gttagggtag caatatcaat cactaattcc
     6421 ggttcaaaac gttctacata gctgagagca tcacatagca ctaaacgtcc ttctgcatcg
     6481 gtgttgagta cctcaacggt taaaccagac attgtagtga gaatatcccc cggtcgatag
     6541 gctttgccat caaccgcatt ttcacagccg gcgagtaagc cgataacatt gagtggtaat
     6601 ttcatctcgg caattgcttt gattgtaccg taaacagtag cagctccgca catatcgtat
     6661 ttcatttcgt ccatcgcctc cgcaggttta atggataaac cgccagagtc aaaggtcatt
     6721 cctttgccga ctaagataat cggcttcgca tcagcattcg ggttgccttt atattcaatc
     6781 actgaaagaa aggcctcatt ttcagaggct ctggaaaccg ctaagtaagc attcattccc
     6841 aattctgcca tctgtttttc atcaacaatg gtagttgaaa tggcaggata gtcgtaggct
     6901 aaacccttag ccaattcggc taaatagtga ggattacaca cattaggcgg acaatttgct
     6961 acatttttag cgtggctgat tccaagtgct acggcttttc cgtgttctaa agctcgttcg
     7021 gcatcaggaa gatctttacg gtgtgcgaca ttaaaaatca cacggcgtaa ttcacggcga
     7081 acttcaggtt tgatgctttt gaaatcgtta taggcatata gttgttcttg gattgcctct
     7141 accgcaaatc gcacattcca ataaggtgtt ctacctttaa cgtgcagttc ggttaaaaag
     7201 ctgacagctt ccattgaacc ggtttcatta atagtttgta cacttttttg aataatttgt
     7261 ttgtattggc gttcagttaa ttcacgctct tttccacagc cgactaataa tactcgatca
     7321 gcgagtacat tcggcacatt atgcaagagt aaggtttgcc ctgctttgcc ttctaaatct
     7381 cctcttctca gtaatgtgct gatatagccg ttacttaatt tatcaagctg ttctgcggca
     7441 gaacataatc ggcggggttc atagacacca acaaccaaac aggcggttct ttgtttctca
     7501 acactgccat ttttgacact aaattccatt tttcctcctt aatttttatt gctacagatt
     7561 ttataatgcc aacataatca tcaatgata
//
LOCUS       DANLZC010000121         7444 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.121, whole genome shotgun sequence.
ACCESSION   DANLZC010000121 DANLZC010000000
VERSION     DANLZC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.121"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(38..832)
                     /locus_tag="PXE44_002020"
     CDS             complement(38..832)
                     /locus_tag="PXE44_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5589497.1"
                     /translation="MALLEVNHLNKRFVERSSLFHKQDFYAVKEVSFTLNRKETLAII
                     GENGAGKSTLAKMVAGMVGPTSGELIFRGTPLSFGDYKYRAKHIRMMFQDPNDAFDPN
                     YNIGQILDAPLRLATDLNEEERNERIFNTLKLVGMYPEQALVPISEASSGQKQRIAFA
                     RALILEPEIIIADDTINSLDFSVKTQLINLMLSVQQRQGIAYIYVGQNLGLIKHIADK
                     IMVMQSGEVVEYGLTKEVLLNPQHQITKRLIESHFGKHLSSYAWAS"
     gene            complement(933..2009)
                     /gene="fbaA"
                     /locus_tag="PXE44_002021"
     CDS             complement(933..2009)
                     /gene="fbaA"
                     /locus_tag="PXE44_002021"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDL5589498.1"
                     /translation="MSLLNIVKPGVVTGDDVQKVFAYAKEHNFAIPAVNCVGSDSVNA
                     VLETAARVKSPVIVQFSNGGAQFYAGKGIKPASGARADVLGAIAGAKHVHQLAEEYGV
                     PVILHTDHAAKNLLPWIDGLLEAGEKHFAETGRPLFSSHMIDLSEEPIEENMEICREY
                     LARMDKIGMTLEIEIGVTGGEEDGVDNSDVDESKLYTQPSEVLYVYDQLSPISPRFTI
                     AAAFGNVHGVYKPGNVKLKPSILGASQEYVSKERGLPAKSLDFVFHGGSGSSREEIRE
                     AISYGAIKMNIDTDTQWAAWDGILQFYKANEAYLQGQLGNPTGPDAPNKKYYDPRVWL
                     RKMEESMSKRLEQSFEDLNCVNVL"
     gene            complement(2106..3281)
                     /locus_tag="PXE44_002022"
     CDS             complement(2106..3281)
                     /locus_tag="PXE44_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL5589499.1"
                     /translation="MSVIKMTDLDLAGKRLFIRADLNVPVKDGKVTSDARIVATIPTL
                     KLALQKGAKVMVTSHLGRPTEGVFEEENSLQPVVDYLNASDLGVPVRLVRDYLDGVEV
                     NENEIVVLENVRINKGEKKNDPELAKKYASLCDVFVMDAFGTAHRAEGSTYGVAEYAP
                     VACAGPLLAAELDALGKALKEPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGI
                     ANTFIAAEGHAVGKSLYEEDLIPEAKRLAQVTNIPVPVDVRVGTEFSETAPATEKAVS
                     EVRADESIFDIGEKSSEQLAEIIRNSKTILWNGPVGVFEFPNFRKGTEVISNAIAEAT
                     ANGAFSIAGGGDTLAAIDLFGIKDKISYISTGGGAFLEFVEGKVLPAVEILEKRANG"
     gene            complement(3536..4894)
                     /locus_tag="PXE44_002023"
     CDS             complement(3536..4894)
                     /locus_tag="PXE44_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL5589500.1"
                     /translation="MANQQQERQSWSNRLAYIMTVAGATVGFGATWRFPYLVGEHGGG
                     AYVFLFCIAMIVIGIPMILVENVIGRRMRVNAIDAFAGFANGKKIASGWKILGYMGLL
                     GAFGILAYYMVLGGWVLNYIGSLLTGTLDLSQPVTVGTTQQFFQQSIFNSPMTIIAYT
                     ALFVLVNYFILVKGIVDGIERSVKYLMPILFIFLIVMVVRNITLPGAAEGIAFYLMPD
                     FSKITPKLFVFVLGQVFFALSLGFGVLITLSSYLDKSENLVKTATITGVMNTLIAVLA
                     GFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSNMWGGTVFAVIFFSLLVVAALTTSLTI
                     YEVIITALQEKAKISRQKAIFITLATIFLLGNIPSALSDNILQDVQVMGKSIFDAFDY
                     ISGNILFILTALGSAIFVGFVLKEEAKEELGAGKMFTTIWFNYVRFVIPVIILVIFFN
                     SL"
     gene            complement(4964..5791)
                     /locus_tag="PXE44_002024"
     CDS             complement(4964..5791)
                     /locus_tag="PXE44_002024"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDL5589501.1"
                     /translation="MNHPKHRAFLKWAGGKYRLIPDIQTHLPKKACLVEPFVGAGSVF
                     LNTDFERYILADINPDLINLFNVVKADVEEYIAQTKQLFLHPQANTEAFYKARRAEFN
                     RSKDIFRRSVIFLYLNRFGYNGLCRYNQKKGYNVPFGRYKTHYFPEKELRFFAEKAQK
                     ATFVCADFEQVFELAKNQLTDYVIYCDPPYAPLAQETNFTQYAGGGFSLEQQGRLATL
                     ALEMKGLGVPVLISNHDTDFTRRIYQSADITQIQVQRSIGQKVGSRVKVGELFALFI"
     gene            complement(5805..6893)
                     /gene="aroB"
                     /locus_tag="PXE44_002025"
     CDS             complement(5805..6893)
                     /gene="aroB"
                     /locus_tag="PXE44_002025"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL5589502.1"
                     /translation="MLQVNVELKERRYPIIIGAGLLNQPASYSPLKSGDKVMIVSNPT
                     VATHYLSVVTNALKELGCHVDSVLIPDGEEYKTLESLNLIFTALLEKNHNRDTTLIAL
                     GGGVIGDVAGYAAASYQRGIRFIQIPTTLLAQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     VSVIVDTNTLHTLSKREVSAGLAEVIKYGAIFDVTFFEWLEKHIDDLVSLKQDELEYC
                     IQRCCQLKADVVARDETEKGDRALLNLGHTFGHAIEARMGYGVWLHGEAVSVGMLEAA
                     ELSRILGDLSPQEVARLEKLLARANLPTISPDGMEPAEYLPYMWRDKKVLAGQLRLIL
                     LKSLGEAYVSSQATESQVLASIERFTQR"
     gene            complement(6917..7438)
                     /gene="aroK"
                     /locus_tag="PXE44_002026"
     CDS             complement(6917..7438)
                     /gene="aroK"
                     /locus_tag="PXE44_002026"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDL5589503.1"
                     /translation="MAEKRNIFLIGPMGAGKSTIGRQLAQTLGMEFMDSDSVIEERAG
                     ADIDWIFDVEGEEGFRKREERILNELTQNYGIVLSTGGGSILSKDNRNVLSARGIVIY
                     LETTVDKQFERTQRDKKRPLLQTEDPRKTLEELAKIRNPLYEEIADITIHTDEQSAKV
                     VANQIIDMIDNLQ"
BASE COUNT         2304 a         1663 c         1292 g         2185 t
ORIGIN      
        1 aattatcaag taatcatatt agagcaggag taaagattta gcttgcccaa gcataggaac
       61 taaggtgctt tccaaaatga ctttcaatta aacgtttcgt aatttgatgt tgcggattca
      121 gtaaaacctc tttcgtcaag ccatactcta ctacttcgcc actttgcatc accattattt
      181 tatcggcaat atgtttgatc agacctaaat tttgccccac ataaatataa gcaatccctt
      241 gtcgctgctg gacacttaac attaaattaa ttaattgtgt tttgactgaa aaatccagtg
      301 aattaatcgt atcatccgca ataataattt ccggttctaa aattaaagca cgagcaaacg
      361 caatacgctg cttttgccca ctggaagcct ctgaaatagg aacgagcgct tgctcaggat
      421 acattcctac caattttagc gtattaaaaa tacgctcatt tctttcctct tcatttaaat
      481 ctgtcgccaa gcggagcgga gcatctaaaa tttgtcctat attataatta ggatcaaagg
      541 catcgttagg gtcctgaaac atcatacgaa tatgcttagc tcggtattta taatcgccaa
      601 atgaaagcgg tgttcctcta aaaatcagtt ctccagatgt tggcccaacc ataccagcaa
      661 ccattttagc caaggtagat ttcccagctc cgttttcacc aataatcgct aaagtttcct
      721 tgcgatttag ggtaaaagaa acttctttta ccgcataaaa atcttgttta tgaaataggc
      781 tactacgttc aacaaaacgc ttattcagat gattaacttc aagtaatgcc ataataaatt
      841 ggtgcttgga aataaagtga tagtttaccg caaaaaataa ataaaaaaag accgcttata
      901 caagcggcct gatttatgga attttttgca aattataaaa cgttcacaca gtttaagtct
      961 tcgaaagatt gctctaagcg tttagacata gactcttcca ttttacgtaa ccaaacacgt
     1021 ggatcgtagt attttttgtt tggtgcatca gggcctgtcg ggttacctaa ttgaccttgt
     1081 aaatatgctt cgtttgcttt gtagaattga agaataccgt cccacgcagc ccattgggtg
     1141 tcagtgtcaa tgttcatttt gattgcaccg tagctgattg cttcacgaat ctcttcacgt
     1201 gaagaaccag aaccgccgtg gaaaacgaag tctaatgact ttgccggtaa accacgctct
     1261 ttagatacat actcttgtga agcacctaag attgatggtt ttaattttac attacccggc
     1321 ttgtaaacac cgtgtacgtt accaaatgct gccgcaatcg tgaaacgtgg gctgattggg
     1381 cttaattggt cgtaaacata taaaacttca gaaggttggg tgtaaagttt agattcatct
     1441 acatcagaat tatctacgcc atcttcttca ccaccggtta cgccgatttc gatttcaagg
     1501 gtcataccga ttttgtccat acgggctaaa tattcacggc agatttccat attttcttcg
     1561 attggctctt cagataaatc gatcatatgt gaagagaata acggacgacc ggtttctgcg
     1621 aagtgttttt cgcctgcttc aagtaagcca tcgatccaag gtaataagtt tttcgcagcg
     1681 tggtcggtgt gaagaatcac aggcacacca tattcttctg ctaattgatg aacgtgtttt
     1741 gcacccgcaa tcgcccctaa tacatctgca cgagcgcctg aagccggctt aatgccttta
     1801 cccgcataga actgcgcacc accatttgag aattgcacga ttaccggtga ttttacacga
     1861 gcggctgttt ctaatacggc attaactgaa tctgaaccca cgcagtttac tgccggaata
     1921 gcgaagttgt gttctttagc ataagcgaaa actttttgta cgtcgtcgcc tgttactaca
     1981 cccggtttta cgatgtttaa taatgacatt tttttcttcc tttgtatgaa atgtgcaaaa
     2041 gcacattgtt aaaatatacg gccaaatctt gcaaaatttt cacaaaattt gaccgcttgt
     2101 aactgttagc cgttagctcg tttttctaag atttccactg ccggtaatac tttaccttcc
     2161 acgaattcta agaacgcacc gccaccggtt gaaatgtaag aaattttgtc tttgataccg
     2221 aataaatcaa tcgcagctaa ggtatcgcca ccaccggcaa ttgagaatgc accgtttgcc
     2281 gttgcttctg caatcgcatt tgaaatcact tctgttcctt tacggaagtt agggaactca
     2341 aacacgccga ccggaccatt ccaaaggatg gtttttgaat tacggataat ttcagctaat
     2401 tgctctgacg atttttcacc aatatcgaaa attgattcat cagctcgtac ttcactcacc
     2461 gctttttccg ttgccggtgc agtttcagag aattcagtac ctacacgcac atcaaccgga
     2521 acaggaatat tggttacttg tgctaaacgt tttgcttcag ggattaaatc ttcttcgtat
     2581 aatgatttac ccacagcgtg accttctgcg gcaataaagg tattggcaat accaccgccc
     2641 acgattaatt ggtcggcaat ttttgaaagc gaatcaagca cagttaattt agtagatact
     2701 ttagaaccac ccacaatcgc taacattgga cgttgtggct cttttaatgc tttgcctaat
     2761 gcatctaatt cagcggctaa taacggacct gcacacgcaa caggggcata ttctgccaca
     2821 ccgtaagttg aaccttccgc acggtgagct gtaccgaatg cgtccattac aaacacatca
     2881 caaagtgatg catatttttt tgctaactca gggtcgtttt tcttctcacc tttgttaata
     2941 cgcacgtttt caagcaccac gatttcattc tcgtttactt caacaccgtc taagtaatca
     3001 cgcactaaac gaacaggaac acctaaatct gatgcattta agtaatcaac tacaggttgt
     3061 aaagagtttt cctcttcaaa tacgccttct gtcggacgac ctaaatgcga agttaccatt
     3121 actttagcac ctttttgtaa cgctaatttt aaggttggaa tggtagcaac aatacgggca
     3181 tctgatgtta ctttgccatc ttttaccggt acgttaaggt cagcacgaat aaataaacgt
     3241 ttacccgcta aatcaaggtc agtcatttta ataacagaca tagtttgttc ctctttagat
     3301 taaaatttaa aagtgataac ggtcggatta tatcagtttc taatcatttt gggctaacct
     3361 agatcaaata aatgattaat tttttatcat tttagaatgc tattatcctg actgttctta
     3421 ttccccccca atatgcttgg ttaattaagc aagaattgca aattccccac aaaatttaac
     3481 cgcttgtcac taaaaataaa gctgtttgaa aatccattca aacagcttat agaatttaga
     3541 gtgaattaaa gaaaatcacc aagataatca ccggaatcac aaagcgaaca tagttaaacc
     3601 aaatagtggt aaacatttta cccgcaccta attcctcctt cgcctcttct tttagaacaa
     3661 agccaacgaa aatcgcgcta cctaaggcgg ttaaaataaa cagaatattg ccactaatat
     3721 aatcgaaggc atcaaaaatg ctcttgccca taacctgcac atcttgcaat atattgtcac
     3781 ttaatgctga tggaatatta cccaacagaa aaatagtagc caaagtaata aaaatagctt
     3841 tttgacggct aatctttgcc ttctcttgca atgcagtgat aataacttca taaatagtta
     3901 acgaggttgt taatgccgcc accactaata gactaaaaaa gataaccgca aaaactgttc
     3961 ccccccacat atttgagaac acaatcggca aactttggaa tactaaagtc ggacctgaat
     4021 ttggagcaac gccaaagcta aataatgacg gaaaaatcat aaaaccggca agtaccgcaa
     4081 ttaaggtgtt catcacccca gtaattgttg ctgtttttac taaattctcg ctcttatcaa
     4141 ggtagcttga aagggttatc aacacaccaa aaccaaggct taatgcaaaa aatacttgac
     4201 ccagcacaaa cacaaacaat tttggtgtga tttttgaaaa atcgggcatc agataaaagg
     4261 caatgccttc agcagccccc ggtaaggtaa tatttcgaac caccatcaca atcaggaaaa
     4321 taaacagaat cggcatcaaa tatttcactg aacgctcaat accgtccaca atgcctttta
     4381 ccaaaataaa ataattcacc aatacaaaaa gtgccgtata agcaataatt gtcattgggc
     4441 tattaaaaat actttgttgg aagaattgtt gggtcgtgcc aacggtaaca ggttgagaca
     4501 gatctagtgt ccccgtgagt aagctaccga tataatttaa tacccaacca ccaagtacca
     4561 tatagtaggc aagaatacca aatgctccaa gcaagcccat atagccaaga attttccaac
     4621 ctgaggcaat ttttttacca ttagcaaaac cggcaaaagc atcaatagca ttcactctca
     4681 ttcggcgacc aattacattc tccactaata tcatagggat accaattacg atcatcgcaa
     4741 tacaaaataa aaacacataa gcaccgcctc cgtgttcccc cacaagataa gggaaacgcc
     4801 aagttgcacc aaaaccgact gttgcaccag caacggtcat aatatacgcc aatcggttag
     4861 accaagattg gcgttcttgt tgttgatttg ccatttaata ctccatcggc taacaatacc
     4921 gcttcaacca acaagcggtc agatttttag aaaatattac aaattaaatg aagagtgcga
     4981 ataactcgcc aactttaacc cttgagccca ccttctgccc tattgaacgc tgtacctgaa
     5041 tttgggtaat atcggcagat tgataaatcc tacgggtaaa atcagtatcg tgatttgaaa
     5101 ttaatacggg aacgcctaat cctttcattt ctaaagccag tgttgccaaa cgcccttgtt
     5161 gctctaggct aaaaccaccg ccagcatatt gcgtaaaatt cgtttcttgt gctaaaggag
     5221 cgtaaggtgg gtcgcagtaa atcacataat ccgttaattg attttttgcc aactcaaaga
     5281 cctgttcaaa atccgcacac acaaaagttg ccttttgtgc tttttcagca aaaaaacgta
     5341 gttctttttc tggaaaataa tgggttttat aacgcccaaa gggaacattg tacccttttt
     5401 tctggttata acggcaaagt ccgttatagc caaatcgatt taaatacaaa aaaatcaccg
     5461 agcgacgaaa aatatctttt gagcgattaa actccgcacg gcgtgcttta taaaaggctt
     5521 ctgtattcgc ttgtgggtga agaaacagtt gctttgtttg cgcaatatat tcctcaacat
     5581 ctgctttcac cacgttgaaa agattaatca agtcaggatt aatatcagca agaatataac
     5641 gttcaaaatc tgtattcaga aagaccgaac ctgcaccaac aaaaggctcg actaaacaag
     5701 ccttttttgg caaatgggtt tgaatatctg gaattaagcg atatttaccg ccagcccatt
     5761 ttaaaaaggc tcgatgctta gggtgattca tttcatttta aacattaacg ttgggtaaaa
     5821 cgctcaatag aggctaatac ttgagattct gtggcttgcg atgaaacgta agcttcacca
     5881 agtgatttca ataaaattag gcgtaattga cctgccagta cttttttatc tcgccacata
     5941 taaggtagat attctgccgg ttccattcca tcaggtgaaa ttgttggcaa attggctcgg
     6001 gccaataatt tttccaagcg agcaacctct tgaggactta aatcccccag aatacgagat
     6061 aattccgcag cctctagcat accaaccgaa acagcctctc cgtgtaacca tacaccatag
     6121 cccatacgtg cttcaatggc gtgaccaaag gtatgtccta agtttagtaa tgcacgatct
     6181 cctttttccg tttcatctcg agcaaccacg tcagctttta gctggcaaca acgctgaata
     6241 caatattcca gctcatcttg cttgagactg accaaatcat caatatgctt ttcaagccat
     6301 tcaaaaaaag taacatcaaa gattgcaccg tatttgatga cttccgccaa accagcactg
     6361 acttctcttt tggataaggt atggagcgta ttggtatcaa caatcacaga aacaggctga
     6421 taaaaagccc caatcatatt tttaccgagt gggtggttta ctgccgtttt cccgcctact
     6481 gaagaatcaa cttgagccag taaagtggtc ggaatttgta taaaacgaat accacgttgg
     6541 taagaggcag ctgcatagcc tgcaacatca ccgattacac caccgcctaa agcaattaaa
     6601 gtggtatcgc gattatgatt tttttctaat aatgcggtaa aaatcaaatt taatgattct
     6661 agcgttttat attcttcacc gtcgggaatc aataccgaat caacgtggca acccagctcc
     6721 tttaacgcat ttgttaccac actaagataa tgtgtcgcca ccgttggatt tgagacaatc
     6781 atcaccttat ctccagattt taatgggcta taactagcag gctgatttaa taaccctgca
     6841 ccaattataa tcgggtaacg acgctctttt aattccacat tgacttgtaa catatcttag
     6901 ctcctttctt acctggctac tgtaaattat caatcatatc aataatttga tttgccacta
     6961 ctttagcgct ctgctcgtcc gtatgaatag taatatctgc aatttcttca tacaatgggt
     7021 tacggatttt cgctaactct tccaacgttt tacgggggtc ttctgtttgc aataaagggc
     7081 gttttttatc tcgctgagta cgttcaaact gtttatccac tgtcgtttct aaataaataa
     7141 cgataccacg agccgataac acattacggt tatctttaga taaaatcgaa ccaccgccag
     7201 ttgaaagcac gataccataa ttttgcgtca gttcatttag aatacgttct tcacgcttac
     7261 ggaaaccctc ttcaccttca acatcgaaga tccaatcaat atccgcacct gctcgttctt
     7321 cgatcacact gtcggaatcc ataaattcca tacctaatgt ttgagcaagc tgacgaccaa
     7381 tcgtactttt acctgctccc atcggcccaa tcaaaaaaat attacgtttt tcagccatta
     7441 tttc
//
LOCUS       DANLZC010000122         7264 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.122, whole genome shotgun sequence.
ACCESSION   DANLZC010000122 DANLZC010000000
VERSION     DANLZC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.122"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            17..517
                     /locus_tag="PXE44_002027"
     CDS             17..517
                     /locus_tag="PXE44_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL5589504.1"
                     /translation="MQTINEIKEEIYPIFEPIIGTIRLKTYFSYYAVFKDGLMIALYQ
                     NGATYLRIAKKDIEYIRQYPKTCILCDEKIGIQSRKFHYIPQVLIDDTPKFSSLLFGI
                     IEELRTEKYKISQKRATQIRNLPNMNLKLERMLKKIGIHSIHDFTQEGYMSTFIKLIL
                     HRRLTV"
     gene            805..2199
                     /gene="fumC"
                     /locus_tag="PXE44_002028"
     CDS             805..2199
                     /gene="fumC"
                     /locus_tag="PXE44_002028"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDL5589505.1"
                     /translation="MEFRIEKDTMGEVQVPADKYWAAQTERSRNNFKIGPEASMPAEI
                     IEAFGYLKKAAAYANHDLGVLAVEKRDLIAKACDEILANKLDDQFPLVIWQTGSGTQS
                     NMNVNEVVANRAHVLNGGKLGEKSIIHPNDDVNKSQSSNDTYPTAMHIAAYKKVVEHT
                     LPCVKRLQKTLAEKAEAFKDVVKIGRTHLMDATPLTLGQEFSAYAAQLAFGIKALENT
                     LPHLRELALGGTAVGTGLNTPQGYDVKVAEYIAKFTGLPFVTAQNKFEALATHDAIVE
                     THGALKQLAVSLYKIANDVRLLASGPRSGIGEILIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDVTISFAGTQGHFQLNVYKPVMAYNFLQSAQLLGDACVSFDEHCAVGIE
                     PNYPRIKQQLDQSLMLVTALNTHIGYENAAKIAKTAHKNGTTLKEEAINLGLVTAEQF
                     DEWVRPEDMVGSLK"
     gene            2326..2436
                     /locus_tag="PXE44_002029"
     CDS             2326..2436
                     /locus_tag="PXE44_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL5589506.1"
                     /translation="MKKLILIALVTTLGLSACGVKGPLYFPEQAPKHEQK"
     gene            2541..3791
                     /gene="lysA"
                     /locus_tag="PXE44_002030"
     CDS             2541..3791
                     /gene="lysA"
                     /locus_tag="PXE44_002030"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL5589507.1"
                     /translation="MNHFEYKNNQLFAEEVAIADIIEQYGTPAYVYSRSTLERHWHAF
                     NNAFGSHPHLVCFAVKSNSNIALLNVMARLGSGFDIVSQGELERVLAAGGEASKVVFS
                     GVAKSHSEIKRALEVGIRCFNIESVSELHRINQVAGELGKIAPISLRVNPDVDAHTHP
                     YISTGLKENKFGVSVTQAREVYRLAKTLPNVKITGMDCHIGSQLTELQPFLDATDRLI
                     ILMEQLREDGIELHHLDLGGGLGVPYNGEEPPHPTEYAKALLEKLKGYENLEIIIEPG
                     RAITANAGILVTKVEYLKQNEDRNFAIVDTGMNDMIRPALYEAYMQITEVDKTLNRDK
                     AVYDVVGPICETSDFLGKQRELAISEGDLIAIRSAGAYGAAMSSTYNSRPQAAEIMVD
                     GNQAHLIKARATFADLWRLEKLLP"
     gene            3805..4434
                     /locus_tag="PXE44_002031"
     CDS             3805..4434
                     /locus_tag="PXE44_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HDL5589508.1"
                     /translation="MKLYGLTGACSFVPHVALEWVKLRANQDYAFQAVSREFIKSAEY
                     LALNPRGNVPLLVDGDLALTQNQAIVHYLDELYPEAKLFGSKTARDKAKAARWLAFFN
                     SDVHKSFVPLFRLPSYAEGNETLTKTIRQQSAEQILEQLAFANAHLENHIFFGEEISV
                     ADAYLYIMLNWCRLLGLDFSHLSQLSAFMQRVEADQGVDNVREQEGLKG"
     gene            4505..5950
                     /gene="tldD"
                     /locus_tag="PXE44_002032"
     CDS             4505..5950
                     /gene="tldD"
                     /locus_tag="PXE44_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDL5589509.1"
                     /translation="MLNQVSNSLLAPSGLELSHLTQVLDHFSGRHIDYADLYFQLSQD
                     ESWSLEDSIIKEGGFYIDRGFGVRAISGEKTGFAYADQITLKQLEQCAAAARSISPTS
                     GQLSVKNFKKTTACSRYPAINPLETISREQKVELLHLVDKTARMADPRVIQVNANLSA
                     VYEEMLVAATDGTLAADIRPLVRLSVSVLVEQNGKRERGSAGAGGRFGLDWFLAPSET
                     GFSRAESLTKEAVRQALVNLTAIPAPAGTMPIVLGAGWPGILLHEAVGHGLEGDFNRK
                     ESSLFSGKIGQMVTSELCTIVDDGTVPNMRGSITVDDEGVPSQRNVLIENGILKGYMQ
                     DKLNARLMGVAPTGNGRRESYAHLPMPRMTNTYLTEGNHEFEEMIESVEYGLYAPHFS
                     GGQVDITSGKFVFSTAEAYLIEKGKITKPVTGATLIGSGIEAMQQVSMVGKKMELDPG
                     IGTCGKEGQSVPVGVGQPTVKLDKITVGGRG"
     gene            6014..6895
                     /gene="prmA"
                     /locus_tag="PXE44_002033"
     CDS             6014..6895
                     /gene="prmA"
                     /locus_tag="PXE44_002033"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL5589510.1"
                     /translation="MAWVQIRLNSTDKQAEEISDFLTEIGAVSVTFMDSQDTPIFEPL
                     PGETRLWGNTDVVGLFDAETDMNLIVEALKTSKLVDENFAHKIEQIEDKDWEREWMDN
                     FHPMQFGKRLWICPSWREVPDPNAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLEGK
                     TVIDFGCGSGILAIAALKLGAKQAIGIDIDPQAILASQNNAEANGVAEKLQLFLAKDQ
                     PRELQADVVVANILAGPLKELAPNIITLVKPNGNLGLSGILATQAESVCEAYSEAFTL
                     DPVVEKEEWCRITGRKI"
     gene            complement(7070..7145)
                     /locus_tag="PXE44_002034"
     tRNA            complement(7070..7145)
                     /locus_tag="PXE44_002034"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7110..7112),aa:Trp,seq:cca)
BASE COUNT         2167 a         1616 c         1618 g         1863 t
ORIGIN      
        1 acatcggagt acatatatgc aaacaatcaa tgaaattaaa gaagaaatct accctatatt
       61 tgagcctatc attggaacaa ttcggctaaa aacctacttt tcctactacg cagtttttaa
      121 ggatggattg atgattgcat tgtatcaaaa tggagcaact tatttacgta ttgcgaaaaa
      181 agatattgaa tacattcgtc agtatcctaa aacctgtatc ttatgcgatg aaaaaattgg
      241 tatacaaagt agaaaatttc attatattcc gcaagttctc atagatgata caccaaaatt
      301 tagctcgcta ctctttggaa ttatagagga actccgaacg gagaagtaca aaataagcca
      361 aaagcgagct acacaaatcc gaaacctacc aaatatgaat ttaaagttag aaagaatgtt
      421 gaaaaaaata gggattcatt ccattcatga ttttactcaa gaagggtata tgtcaacttt
      481 tatcaaactg attctccacc gaagacttac tgtttaagct aaacggagct ttaaatcacc
      541 aatatattta caccttcaca gatcaacaaa aaagggaact aatgcaagaa gcaaatcaag
      601 cgctatataa ttcaggccta agaaaaagat ttaacgccct gtagttaaat aatgtgatcc
      661 aaatcataaa atataaaaaa accaataagg aattgcaatt cgcagttcct ttctttttct
      721 tttatatgct agtatcacct tatacctcta aattattagt gcctactgat tttcaattaa
      781 tccaacaatt taggagagcg catcatggaa ttccgtatcg aaaaagacac aatgggcgaa
      841 gtgcaagttc ctgccgataa atactgggcg gcgcaaaccg agcgttcacg caataacttc
      901 aaaatcgggc cggaggcatc aatgccggcg gaaattatcg aggcgtttgg ctatctgaaa
      961 aaagcggctg cctatgctaa tcacgattta ggcgtactag cggtcgaaaa acgggattta
     1021 attgcaaaag cctgtgatga aattttggcg aacaaactcg atgatcaatt cccgctggtg
     1081 atttggcaaa ccggctcggg tacgcagtca aatatgaacg tgaatgaagt cgtggcaaac
     1141 cgtgcacacg tgttaaacgg cggcaagctg ggtgaaaaat cgattattca cccgaatgat
     1201 gatgtgaata aatcccaatc ttctaacgac acctacccaa ctgcaatgca catcgctgct
     1261 tacaaaaaag tggtggaaca caccctacct tgtgtgaaac gtctgcaaaa aaccttagcg
     1321 gaaaaagccg aagcctttaa agatgtggtc aaaatagggc gtactcactt aatggatgct
     1381 accccgctca cgctcggtca agagttctct gcctatgcag cacagttagc attcggcatc
     1441 aaagcattgg aaaacaccct gccacacctg cgtgagctgg cactcggcgg cacagcagtc
     1501 ggcaccggct tgaatacccc gcaaggttac gatgtgaaag tggcggaata cattgcgaaa
     1561 ttcaccggtt taccgttcgt taccgcccaa aacaaatttg aggcattagc gactcacgat
     1621 gcgattgtcg aaacccacgg tgcattaaaa cagcttgcgg tgtcgctcta caaaattgcc
     1681 aacgatgtcc gtttattagc ctccggtcct cgttcaggca ttggcgaaat tctgattccg
     1741 gaaaacgagc cgggttcatc aattatgccg ggtaaagtaa acccaaccca atgcgaagca
     1801 atgacaatgg tatgtgccca agtattaggc aacgatgtaa ccatctcttt tgccggcacg
     1861 caagggcatt tccaactgaa tgtctataaa ccggtgatgg cgtacaactt cctgcaatcg
     1921 gctcaactgc taggcgatgc gtgcgtgtcg tttgacgaac actgtgcagt cggcattgaa
     1981 ccgaactacc ctcgcattaa acaacagctc gaccaatcgc tgatgttagt gaccgcatta
     2041 aatactcaca ttggttatga aaatgcagcg aaaatcgcca aaactgccca caaaaacggc
     2101 acaacactga aagaagaggc gattaactta ggcttagtca cggcagaaca gttcgatgaa
     2161 tgggttcgtc cggaagatat ggttggcagc ttgaaataag cccaaccctt ctcttgtaat
     2221 ctgatttaca cctcaatcta ccggtaagat tgaggtgtaa ttttttatag cgagggtact
     2281 gattttgttt tagaatgaag catcattttt taagcggaaa aacctatgaa aaaactcatt
     2341 ttaatcgcat tagttactac tttaggctta agtgcttgtg gggtcaaagg cccactctac
     2401 ttccctgagc aagcccctaa acatgaacaa aaataagcat tgcaagcggt ctgttttggc
     2461 taaaattttg caaatttttg tgaaaaaatg accgcttgtt gcctcttttc tttcaaaatc
     2521 ccaaacacaa cggactttca atgaaccatt ttgaatataa aaacaaccaa ctttttgctg
     2581 aagaagtcgc tattgccgat attatcgagc aatacggcac gccagcttat gtttactcca
     2641 gatctaccct tgaacgccac tggcacgcat tcaataatgc gtttggctcg cacccacatt
     2701 tagtttgctt tgcagtcaaa tccaactcta acattgcctt gttaaatgta atggctcgtc
     2761 tcggttcagg ctttgatatt gtctctcaag gcgagttgga gcgtgtctta gctgccggcg
     2821 gcgaagcctc taaagttgtc ttttccggtg tggcaaaatc gcacagcgaa atcaaacgag
     2881 cattggaagt cgggattcgt tgctttaaca ttgaatcggt ttccgaactt caccgcatta
     2941 accaagttgc cggtgaacta ggcaaaatcg caccgatttc actgcgggta aacccggatg
     3001 tggacgccca cactcaccca tacatttcca ccggtttaaa agaaaacaaa ttcggcgtga
     3061 gcgttactca agcccgtgaa gtgtatcgct tagccaaaac cttaccgaat gtgaaaatta
     3121 ccggtatgga ttgtcacatc ggttcgcaat taaccgaatt gcaacctttt ttagatgcaa
     3181 cggatcgctt aatcatctta atggagcaac tgcgtgaaga cggcattgag ctacaccact
     3241 tagacttagg tggcggttta ggcgtgcctt acaacggcga agagcctccg catccgaccg
     3301 aatatgccaa agccttgctg gaaaagctca aaggctatga aaacttagaa atcattattg
     3361 aaccggggcg tgccattacc gcaaatgccg gtattttagt gacgaaggtg gaatacttga
     3421 aacaaaatga agatcgtaat tttgcgattg tcgataccgg tatgaacgat atgattcgcc
     3481 cggctctgta tgaagcctat atgcaaatta cggaagtgga taaaacctta aaccgtgaca
     3541 aagctgttta cgatgtggtc ggtccgattt gcgaaacctc ggatttctta ggcaaacaaa
     3601 gagaattggc aatttctgaa ggggatttaa tcgctattcg ttctgccggt gcttatgggg
     3661 cggcgatgtc ttcaacctac aactctcgcc cacaagcggc agaaattatg gttgatggca
     3721 accaagccca tttaattaaa gcccgagcca cttttgctga tttatggcgt ttagaaaaat
     3781 tattacctta atgaggaaga aaagatgaaa ttatatggat taaccggtgc ttgttcattt
     3841 gtaccacacg ttgccctaga gtgggtgaaa ttgcgtgcca atcaagatta tgcgtttcaa
     3901 gctgttagcc gtgaatttat taaatcggcg gaatatctgg ctttaaaccc tcgtggtaac
     3961 gtaccattgt tggttgatgg cgacttggcc ctcacgcaaa accaagctat cgtccactat
     4021 ttagacgagc tttatccgga agctaaatta ttcggcagca aaaccgctcg tgataaagcc
     4081 aaagcggctc gttggttagc ttttttcaat tcagatgtgc ataaatcatt cgtgccactg
     4141 ttccgcttgc caagttatgc ggaaggcaat gaaacgctca ccaaaactat tcgccaacaa
     4201 tcggctgagc aaattttaga acaactggct tttgcgaatg ctcatttaga aaatcatatt
     4261 ttctttggcg aagaaatttc ggttgccgat gcgtatctct acattatgct caactggtgt
     4321 cgcctactag ggttggattt ctctcatctt tcccaacttt ctgcctttat gcaacgagtg
     4381 gaggcagacc aaggcgtaga taatgtgcgt gagcaagaag gcttaaaagg ttaattcacc
     4441 attgttcatt taagacggta gattttatgt taaaatctac cgtcttttat ttttaggaat
     4501 caccatgcta aatcaggttt caaacagctt gcttgctcca agtgggcttg aattatcgca
     4561 cttaactcaa gtgttagatc acttctccgg ccgtcatatt gattatgccg atctctactt
     4621 ccaactcagc caagacgaaa gctggagctt ggaagacagc atcatcaaag aaggcggttt
     4681 ttatatcgac cgagggttcg gtgtgcgagc catatcaggc gaaaaaaccg gctttgccta
     4741 cgccgatcaa atcacgttaa aacagcttga gcaatgtgcc gctgccgcac gttcaatcag
     4801 cccaacaagc ggtcaattat cggtaaaaaa tttcaaaaaa acgaccgctt gttctcgcta
     4861 cccggcaatc aacccgctag agaccatcag ccgtgaacaa aaggtcgaat tgctccattt
     4921 ggtggataaa accgctcgaa tggcagatcc gagagtgatt caagtgaatg cgaatctctc
     4981 agcggtatat gaagaaatgt tagtggcagc gactgacggc acactggctg cggatattcg
     5041 cccgttagtg cgtttatctg tgtcggtact ggtggaacaa aatggcaagc gtgaacgagg
     5101 ttcagcaggt gccggtggtc gctttggttt agattggttc ttagcaccaa gcgaaaccgg
     5161 ctttagccgt gcggaatcgc ttaccaaaga agccgttcgc caagccctcg ttaatctcac
     5221 agccattcct gcaccggcag gcacgatgcc gattgttctc ggtgctggct ggccgggcat
     5281 tttgctccac gaagcagtcg gacacggctt ggagggtgat tttaaccgta aggaatcttc
     5341 gctcttcagt ggtaaaatcg ggcaaatggt cacctctgag ctttgcacca ttgttgatga
     5401 cggtacggtg ccgaatatgc gaggctcaat cacggtagat gacgaaggcg tgccttcgca
     5461 acgtaatgtg ttaattgaaa acggcattct aaaaggctat atgcaagaca aactcaatgc
     5521 ccgcctgatg ggagttgccc caaccggcaa cggcagacgg gaatcttacg ctcatttgcc
     5581 gatgccacga atgaccaaca cctacctcac cgaaggcaat cacgaatttg aagagatgat
     5641 tgaatcggtc gaatacggtt tatatgctcc tcacttcagt ggtggacaag tggatattac
     5701 ctccggcaaa tttgtgttct ccaccgccga agcctactta atcgaaaaag gcaaaatcac
     5761 caaacctgtt accggtgcaa ccctgatcgg cagcggcata gaagcaatgc aacaagtttc
     5821 aatggtcggc aaaaaaatgg aactcgaccc cggcatcggc acttgtggca aagaagggca
     5881 atccgtacca gtcggcgtag ggcagcctac agtgaagttg gataaaatta cggtgggggg
     5941 aagagggtaa tctctccccg tttgcaaaat ataaagaaaa tatcaccgct tgtagcgata
     6001 aaaggaaaac aaaatggctt gggtacaaat tcgtttaaac agtacagata aacaggctga
     6061 ggaaatcagc gattttttaa ccgagattgg agcggtgtca gtgactttta tggatagtca
     6121 agatacccca atttttgaac ctcttccggg ggaaacccgt ctttggggaa ataccgatgt
     6181 ggttgggctt ttcgatgcgg aaactgatat gaatcttatc gtagaagcac taaaaaccag
     6241 caaattggtg gacgaaaact ttgctcacaa aatcgagcaa atcgaagaca aagactggga
     6301 acgggagtgg atggataatt tccacccaat gcagtttggc aaacggttat ggatctgccc
     6361 aagctggcgt gaagtaccag acccgaatgc agtgaatgtt atgttagacc ctggtttagc
     6421 atttggcacc ggcacacacc caacgacctc gctttgctta caatggttag atagcttaga
     6481 tttagaaggc aaaacggtta tcgacttcgg ctgtggctcc ggtattttag ctatcgctgc
     6541 acttaaactc ggtgcaaaac aagccatcgg gattgatatc gacccacagg cgattttagc
     6601 tagtcaaaac aatgctgagg cgaacggcgt agcagaaaaa ctacaactat tcttagccaa
     6661 agatcagcca agagagttac aagccgatgt cgttgttgcg aatattctag ccggcccact
     6721 caaagagtta gcaccgaata tcatcacgct ggttaaacca aacggcaatt taggcttatc
     6781 cggcattctt gctactcagg ctgaaagtgt gtgtgaggct tatagcgaag cctttacgct
     6841 agaccctgtt gtagaaaaag aagaatggtg tcgtattaca ggccgcaaaa tctaatataa
     6901 gaagccctgt tttaaacagg gcttactttt tgataaattc tactactctt tacctgtctt
     6961 gggaatcgtt ctccctaaat taattaacac aagcggtaga aataacagga aaatttacaa
     7021 attacataaa tgaaaaaagc cgactgaaat cagtcggctt tctaaataat ggcaggggcg
     7081 gagaggctcg aactcccaac acccggtttt ggagaccggt gctctaccaa ttgaactacg
     7141 cccctattga taggtattca ttttgcactt ataggggcga attactattc gcccctgcat
     7201 taaagattat tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg
     7261 tatt
//
LOCUS       DANLZC010000123         7261 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.123, whole genome shotgun sequence.
ACCESSION   DANLZC010000123 DANLZC010000000
VERSION     DANLZC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.123"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..336)
                     /locus_tag="PXE44_002035"
     CDS             complement(<1..336)
                     /locus_tag="PXE44_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5589511.1"
                     /translation="MIITMKDIAKEANVSLGTVSNVLNGKSNVSLAKIEKVQAAVEKL
                     GYRKNIQASTLKSGTSNKIAIILPNITELKYACLYENLDLICTQNDLSLILYLTHNRE
                     DREKQIIQNI"
     gene            complement(498..1838)
                     /gene="gdhA"
                     /locus_tag="PXE44_002036"
     CDS             complement(498..1838)
                     /gene="gdhA"
                     /locus_tag="PXE44_002036"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL5589512.1"
                     /translation="MSDLNALFQKIKQRDPNQAPFHQAVEEVFGSLAPFLAKNPKYTQ
                     QGLLGRIVEPERVISFRVTWVDDKGQVQVNRGYRVQMNSAIGPYKGGIRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADTD
                     VPAGDIGVGGREIGYMFGQYKKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFAES
                     MLATKGQQIEGKSVVISGSGNVAQYAAEKVIQKGGKVLTVSDSNGYVLFPESGMTEAQ
                     LQALLELKNERRERLSVYATEQGLKYFDGQKPWGVKCDIALPCATQNELDTEDAKELV
                     KNGCICVAEGANMPTTLGGVEVFIGAKILYAPGKAANAGGVATSGLEMSQNAIRLSWS
                     REEVDQRLFNIMKAIHENCVENGTEADGFVNYVNGANIAGFKKVANAMLEQGIV"
     gene            complement(2031..2372)
                     /gene="bamE"
                     /locus_tag="PXE44_002037"
     CDS             complement(2031..2372)
                     /gene="bamE"
                     /locus_tag="PXE44_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDL5589513.1"
                     /translation="MKMKSLLAAMLLALGVTACSTVEKVVYRIDVPQGNYLEQDKIAQ
                     LKVGMTAEQVEYLLGTPMLKNIFETHRWDYVFIKREGYKDPEQRNLFIYFDAKGLVSK
                     IDDSNANAEQK"
     gene            complement(2439..3440)
                     /gene="yejK"
                     /locus_tag="PXE44_002038"
     CDS             complement(2439..3440)
                     /gene="yejK"
                     /locus_tag="PXE44_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDL5589514.1"
                     /translation="MSINVSEIVLHQLQKTEGENPELNTFLRENLLAISPEVDQMMLQ
                     LHQAYQSKAKAYGIFKPESVFAQQLNRLLEQETDFLPFSHSCAKMLAAELIKYPFADG
                     GTFILCRYTFLATEYLFIALIDSRNSMLVDEQLEIKRTQYLEIAQYDIACRINLTELK
                     LDATSNRYLTFVKGRVGRKIADFFMDFLSAEEGFNPQLQNQTLLQAVSDYCEQGELSA
                     PQTREVKKQVFEYCKGQINSGEEIALNELSNALPTLNEIDFAQFTQEQEYGLEENIPP
                     MRNALKTLTKFSGSGKGVTISFNAELLGERLIWDELNDTLTIKGLPANLRDQLERNK"
     gene            3521..3907
                     /locus_tag="PXE44_002039"
     CDS             3521..3907
                     /locus_tag="PXE44_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL5589515.1"
                     /translation="MTVIHTENAPAAIGPYVQAVDLGNMVLTSGQIPVNPETGEIPSD
                     IVQQTRQSLNNVKAIIEQAGLTVADIVKTTVFVKDLNDFAKVNAEYQAFFQENEHPNF
                     PARSCVEVARLPKDVGIEIEAIAVRR"
     gene            3939..4676
                     /gene="pgeF"
                     /locus_tag="PXE44_002040"
     CDS             3939..4676
                     /gene="pgeF"
                     /locus_tag="PXE44_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDL5589516.1"
                     /translation="MDKIIPNWNVPKHIHALTTLRTGGVSKPPFDSFNLGDHVNDEPQ
                     DIAQNRALLVEKFQLPQAPLFLTQTHSTRVIELPYSGDNIKADAIYTNQPNQVCLVMT
                     ADCLPVLFASQDGSEIAAAHAGWRGLCDGILEATVEKFNCPPHEISAWLGPAIGPNAF
                     QVGSEVADQFCAFDPRAKEALIEDSTTSGKFLGNLYQIATQRLNKLGITAISGGEYCT
                     YSQPELFFSYRRDKQTGRMATLIWRTE"
     gene            4770..4845
                     /locus_tag="PXE44_002041"
     tRNA            4770..4845
                     /locus_tag="PXE44_002041"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4803..4805,aa:Ile,seq:cat)
     gene            5048..6262
                     /locus_tag="PXE44_002042"
     CDS             5048..6262
                     /locus_tag="PXE44_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5589517.1"
                     /translation="MTATKKTLSDTLLKNLKYSPNAKPIADSNGLFILAQKKAKVWIY
                     SYISPKTGKRSTKNRIGSYPQMSLAEARLKRDELNTLVLQGLDPFEHLAKMQREAKEK
                     QESIQSFAYKWADWKLSKGKCKPKTMEKCLQRLENHLFPRFEGYTLEDFTLTDTINRL
                     TDLDRIMPDTLHRIAGNLIEILDYAVLCGRINFNPISVIKKAFSSVSTTHQPAIMPEE
                     LAEFMQDLQKSNRTPQIKLLVEWQLLNILRPFEAVAVQWSDIDWETKTLNIPADRMKG
                     GKNPHSVPLTKQAIAILEEMKKYNGHHKHIFTHRTDRNKPANSQTVNNAIKRLSGGKY
                     KGLLVAHGLRSIASTYLHERFTTETLVIEACLSHTNKDTVMSAYHRKNWLDRRRGIMQ
                     EWADFVEQCKSI"
     gene            6732..6938
                     /locus_tag="PXE44_002043"
     CDS             6732..6938
                     /locus_tag="PXE44_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDL5589518.1"
                     /translation="MENTPAKQRFMKIDEVSAVTTLSETQIYRKMKAGDFPQSISIGA
                     NSKVWLESEIQQWIQRKIEQHRNQ"
     gene            6948..>7261
                     /locus_tag="PXE44_002044"
     CDS             6948..>7261
                     /locus_tag="PXE44_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589519.1"
                     /translation="MTLETFNQQKAKFHEQEKAVLAIQTELDKAKNILEALQNEKAEF
                     VARQKTNLANIGTLSADEYVEIKNKNSGLQARIEYYQALIVDLENKCYAEQEILFQQQ
                     NEL"
BASE COUNT         2328 a         1556 c         1308 g         2069 t
ORIGIN      
        1 tatattttga ataatttgtt tttctctatc ttctcgatta tgcgttaaat aaagaatgag
       61 agataaatca ttttgcgtac aaattaaatc aagattttca tataaacagg catatttcag
      121 ctctgtaata ttgggcaata taatagcaat tttattactt gtgccacttt ttaacgtaga
      181 ggcttgaata tttttacgat aacccaactt ttcaacggca gcttgtactt tttctatttt
      241 agctaagcta acattacttt ttccgtttaa tacattcgat actgttccta acgaaacatt
      301 tgcttccttt gcgatatctt tcatcgtaat gatcatatac tgccctttca catactgcta
      361 aaactgagat tggcatagat tttaccataa ttggcaaatg aaatacgatt tcaatttagc
      421 ttgtctctag ctacaaccta aaataaaaaa actgcctcga tatatcgagg cagttcttga
      481 ataataggga taataaatta aacaataccc tgttctaaca tcgcatttgc cacttttttg
      541 aaaccagcaa tattcgcacc gtttacatag tttacgaaac catctgcttc agtaccattt
      601 tctacgcagt tttcgtgaat tgctttcata atgttgaata aacgctgatc tacttcttca
      661 cgactccaag atagacgaat tgcattttgg ctcatttcta aacctgaggt tgccacacca
      721 cctgcgttag ccgctttacc cggagcataa aggattttcg caccgataaa gacttcaaca
      781 ccacctaatg tggttggcat atttgcacct tctgccacac aaatacagcc gtttttcact
      841 aattctttag catcttcggt atctaattcg ttttgggttg cacaaggtaa agcgatgtca
      901 catttcacac cccacggttt ttgaccgtca aagtatttta agccttgttc tgttgcatac
      961 acagataagc gttcacggcg ttcgtttttc aactctaata atgcttgtaa ttgtgcctct
     1021 gtcataccgg attcagggaa taagacataa ccgttagaat ctgaaaccgt taatacttta
     1081 ccgccttttt gaatcacttt ttcagctgca tattgtgcca cgttacccga accggaaatt
     1141 accactgatt ttccttcgat ctgctgacct ttagtcgcta acatagactc tgcaaaatac
     1201 accgcaccat aaccagttgc ttccggacga attaagctac caccccaagt cagcgattta
     1261 ccggttaata ctgaagtaaa ttcattgcgt aattttttgt attgaccgaa catatagccg
     1321 atttcacgac ctcccacgcc gatatcgcct gcaggaacgt cagtatcagc accaatgtgg
     1381 cgatataatt cgctcataaa cgcttggcag aaacgcatta cttcggcatc agatttgcct
     1441 ttcgggtcaa agtctgaacc acctttacca ccgcccattg gcaatgtcgt taacgcattt
     1501 ttgaatactt gctcaaaggc taagaatttt aatacgccta aatcaacggt tgggtggaaa
     1561 cgaataccgc ctttgtatgg accgattgct gagttcattt gtacacggta accacggttt
     1621 acttgcactt gacctttatc atcaacccaa gtgacacgga agctaattac acgctcaggc
     1681 tcaacaatac gccctaataa accttgctgg gtatatttcg gatttttagc taagaaaggg
     1741 gcaaggctac cgaacacttc ttcaaccgct tgatggaaag gggcttggtt cggatcacgt
     1801 tgtttgattt tttggaaaag ggcgtttaaa tcgctcatat tatactcctg aataaattga
     1861 tgatgttgtg tatttaagat gagcgaagtc tagcaagcaa ttttagcttt gaaaagatag
     1921 attagaaaaa tttatcatta aaataagaaa aattaagttt aaatgcttat tttttattca
     1981 aaaaaataag cccactttta aaagtgggct tttcgaacca acaaatcgtc ctatttttgt
     2041 tctgcattcg cattgctgtc atcaattttt gatactaacc ctttagcatc aaaataaata
     2101 aacagattgc gttgttcagg atctttataa ccttcacgtt taataaacac ataatcccaa
     2161 cggtgggttt caaaaatgtt ctttaacatt ggtgtaccaa gcaaatattc aacttgttcc
     2221 gctgtcatac cgactttaag ttgagcaatt ttatcttgtt ctaagtaatt gccttgcggt
     2281 acatcaatac ggtacaccac tttctcaacg gttgaacaag ccgttacacc cagtgcaagc
     2341 aacattgcag ctaaaagaga tttcattttc ataattgacc ttttgataat ccaaaattca
     2401 gataacgaga taatacctaa actgataaaa attttccatt atttgttacg ttccagctgg
     2461 tcacgcagat ttgccggtaa tcctttgatt gtcagtgtgt cgtttagttc atcccaaatt
     2521 aaacgctctc ctaataattc tgcgttaaag ctaatcgtta ctcctttgcc cgaaccggaa
     2581 aatttcgtca gggttttaag ggcatttcgc ataggcggaa tattctcttc caagccgtat
     2641 tcttgctctt gcgtaaattg ggcaaaatcg atttcattca gtgttggcaa tgcgttggaa
     2701 agctcattta acgcaatttc ctctccacta tttatctgcc ctttacaata ttcaaacacc
     2761 tgctttttca cttctcgagt ttgaggggca gataactccc cttgctcaca ataatcgctc
     2821 accgcttgca acaaggtttg attttgcaat tgtggattaa acccttcttc cgcacttaaa
     2881 aaatccataa agaaatcggc aattttacgc cctactctgc ctttcacaaa agttaaatag
     2941 cgatttgacg ttgcgtcaag tttcagttca gttaaattaa tgcggcaggc aatatcatat
     3001 tgtgcaattt cgagatattg cgtgcgttta atctctaact gctcatcaac cagcattgaa
     3061 ttacggctat caatcaacgc aataaacaaa tattcggtag ctaaaaaagt gtaacgacac
     3121 aaaataaaag tgccaccatc ggcaaacgga tatttaatca gctctgctgc aagcattttg
     3181 gcacagctat ggctaaaagg taaaaaatcg gtttcttgct ccaacaagcg gttgagctgt
     3241 tgagcgaaaa ccgactccgg tttaaatata ccgtaagcct ttgctttact ttgataggct
     3301 tgatgtaatt gcaacatcat ttgatcgact tcaggggaaa ttgccaataa attttctcgc
     3361 aaaaacgtat taagctccgg gttctcgccc tctgtttttt gtaattgatg taacacgatt
     3421 tcagaaacat taatgctcat taattcactt ctctttattc caaataataa gaaaagatat
     3481 tatcattaat ccgtttattt ttcattaaga ggaacacaag atgacagtta tccacacaga
     3541 aaatgcaccg gcagcgattg ggccttatgt gcaagcagtt gatttaggca atatggtttt
     3601 aacttctggg caaattcccg tgaatcctga aaccggcgaa atcccgagtg atattgtgca
     3661 acaaacccgc caatctctga acaacgtgaa agccattatc gaacaagccg gcttaaccgt
     3721 tgccgatatt gtaaagacca ccgtatttgt caaagatctt aacgacttcg caaaggtaaa
     3781 tgcggaatac caagccttct tccaagaaaa cgaacaccct aattttccgg ctcgttcttg
     3841 cgtagaagtg gctcgtttac caaaagatgt tggcattgag atcgaagcga ttgcagtacg
     3901 ccgataacaa gcggtcattt tcttgagatt ttttgcaaat ggataaaatt attccaaatt
     3961 ggaatgtgcc taaacatatt cacgcactga cgaccttaag aacaggcgga gtaagcaaac
     4021 cgccttttga cagtttcaac ttaggtgatc acgttaatga cgagccacaa gatatcgctc
     4081 aaaatcgtgc tttattggtt gagaaatttc aattaccaca agctccgctg tttttaaccc
     4141 aaactcacag cacccgagtg attgaacttc cctattcagg tgataatatt aaagccgatg
     4201 cgatttacac caatcaacca aaccaagtct gcttagtgat gactgcagat tgtttgccgg
     4261 tattatttgc cagccaagac gggtcggaaa ttgcggcggc acacgccggc tggcgtgggt
     4321 tatgcgatgg catattagag gcgacggtag agaaattcaa ctgcccgcca cacgaaatat
     4381 cagcttggct agggccggct atcggaccga atgcttttca agtcgggagt gaagtggcag
     4441 accaattctg tgcttttgac ccaagagcga aagaagccct tattgaagac agcactacaa
     4501 gcggtaaatt tttaggaaat ctttaccaaa ttgccaccca acgtctgaat aaattaggca
     4561 ttaccgccat ttcaggcggt gaatattgca cctacagcca accagaactg tttttttcct
     4621 accgccgaga caaacaaacc gggcgaatgg caaccttaat ttggcgaaca gaataaccct
     4681 attttctctc gttcaataat taaccgagta atcttctgcc tctagacgac tactcggttt
     4741 ttctctataa ttccaaccac ttttcaaatc cccccatagc tcagtggtta gagcaggcga
     4801 ctcataatcg cttggtcgct ggttcaagtc cggctggggg gaccatctaa gcgttgcata
     4861 ttgtctttca ttgtctcaaa tttactataa aatcaagaac ttagattctt ttatagtctc
     4921 ttattgtctt ctttagttgc attgtatctt agtgtttatg atccctaaaa tgatccctat
     4981 tttgaaaacg ggaaaattta gggatcataa acttcgataa acccttatat atcaaggatt
     5041 agaagaaatg actgcaacta aaaaaacact ttcagacaca ttactaaaaa atctcaaata
     5101 ttccccaaat gcgaagccta tcgcagacag caacgggcta tttattcttg cacagaaaaa
     5161 agcgaaagta tggatttata gctatatcag ccccaaaaca ggcaagcgaa gcacaaaaaa
     5221 tagaatcggt agctatccgc aaatgagctt agccgaagca cgcttaaaga gagacgaact
     5281 aaataccctt gttttacaag gcttagaccc tttcgagcat ttagccaaaa tgcaaagaga
     5341 agctaaggaa aagcaagaat ctattcaatc tttcgcctat aaatgggctg attggaagct
     5401 atcaaaggga aaatgcaagc ctaaaacaat ggaaaaatgc ttacagcggt tagaaaatca
     5461 tttattccca cgttttgaag gctatacgct ggaagatttt accttaaccg atacaatcaa
     5521 ccgcttaacg gacttagatc ggataatgcc cgacactttg cacagaatag cagggaattt
     5581 aatagaaatt cttgattatg cggtattgtg tgggagaatt aattttaacc caatcagcgt
     5641 cattaaaaag gcgttttcca gcgtaagcac gacacaccag ccagcaataa tgcccgaaga
     5701 gttagccgaa tttatgcaag acttgcagaa atcaaataga acgcctcaaa ttaaattatt
     5761 agttgaatgg caactcttga atattcttag accgtttgaa gccgtagcgg tgcaatggag
     5821 cgatatagat tgggaaacta agacgctcaa cattccggca gatagaatga aaggcggaaa
     5881 aaatccgcat tctgtaccgc taacaaaaca agctatcgca atcctagagg aaatgaagaa
     5941 atataacgga caccataaac atatttttac acaccgaaca gaccgaaaca aaccggctaa
     6001 tagccaaacc gtgaataatg caataaaacg cttaagcggt ggaaaatata aagggctttt
     6061 agtggctcac ggtttaagaa gcattgccag cacctattta cacgaacgtt ttactactga
     6121 aacgctagta attgaggctt gtttatccca cacgaacaaa gacaccgtaa tgagtgcata
     6181 ccaccgtaaa aactggctag accgcagacg tggaataatg caggaatggg cggattttgt
     6241 agaacaatgc aagagcattt aaatgacaaa ggcggagcaa tccgcctttc tttttgcttg
     6301 atgaaaaatt ttcatttttt cctatcagct cccagcccgt gccgtctatc ccacgcctcg
     6361 cccgcacact taaagtctcg atttctacgc aaatgcaaaa ttaagcccaa acattgcccc
     6421 gtatagcgtg aaaaactttc atatagccgt tttttattac gcaattttac gcaagaaaac
     6481 gcaaatttac gcaggcagaa acaccacttt taaaaactcg atgacagaac gatgaaagaa
     6541 ccgctgaaaa cttgcaaaaa atcagagaaa aataaccgct tgcaagtttt ccaaatcaga
     6601 attaagcccc tctaatctgt aaaatctcct ttcacagccc taacaaatag caaaaaatcc
     6661 aagatgattt attctttcac acgttagcag gcaatagcac gccaaagcaa atttttacgg
     6721 agtaaaacaa aatggagaac acaccagcaa aacagcgatt tatgaaaata gatgaagttt
     6781 cagccgtaac tacgctttca gaaacgcaga tttatcgcaa aatgaaagcc ggcgattttc
     6841 cccaaagtat cagtattggg gcaaatagca aagtatggct agaaagtgaa atccagcaat
     6901 ggattcaacg caaaattgaa caacaccgca accaataagg gcaaattatg acacttgaaa
     6961 catttaacca acaaaaagct aaattccacg aacaagaaaa agccgtttta gctattcaga
     7021 ccgaattaga caaagcgaaa aatattcttg aggcattgca aaacgaaaaa gcggaatttg
     7081 tcgcacggca aaaaacgaat ttagccaata tcggcacgct ttcagcagat gaatatgttg
     7141 aaatcaaaaa taaaaatagt ggtttacaag cacgcataga atattatcaa gcgttgattg
     7201 ttgatttaga aaataaatgc tacgcagagc aagaaatatt attccaacag cagaatgaac
     7261 t
//
LOCUS       DANLZC010000124         7177 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.124, whole genome shotgun sequence.
ACCESSION   DANLZC010000124 DANLZC010000000
VERSION     DANLZC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.124"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            76..1167
                     /gene="ulaG"
                     /locus_tag="PXE44_002045"
     CDS             76..1167
                     /gene="ulaG"
                     /locus_tag="PXE44_002045"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL5589520.1"
                     /translation="MSKIQEITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     CVGVWIKTPAGANICMDLWCGRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVL
                     DPFAINEVDFILASHYHSDHIDVNVAAAICNNPKLDHVKFVGPWHCAELWKKWGVPEE
                     RIIVVKPGDVVKLKDVEIHALDSFDRTCLVTLPVEGAEERGGELAGLCPSDEEMGRKA
                     VNYVFKTPGGTIYHGADSHYSIQFAKHGKQFDIDVALNNYGENPVGIADKMTSVDLLR
                     MAECLRCKVIIPVHHDIWTNFMASTQEIIDLWRMRKDRLQYQFHPFIWEVGGKYVYPR
                     DKDLIEYHHPRGFDDCFEQEPNIQFKSML"
     gene            1251..1997
                     /gene="ulaR"
                     /locus_tag="PXE44_002046"
     CDS             1251..1997
                     /gene="ulaR"
                     /locus_tag="PXE44_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDL5589521.1"
                     /translation="MNESYRHKELLALLEERKALSTADIMQLLNISPATARRDISKLD
                     GQGKLKKVRNGAEAILAPTFLQHTKITNNAQEKRRIAEAAGRLCKDGESLILTCGSTM
                     EMLGEVLCGRKLQIITNYLPLANQLIAGCHDDVVIMGGQYNKHKKVMLSINTQNEMTY
                     AATTMFTSGKGLTSEGLFKTDMIIANAEQQMAKHAERYVVLLDSTKLGKKVGMLFKEF
                     DKIDLLITGKEADPTIIQALRNKGLDIILA"
     gene            2052..2912
                     /locus_tag="PXE44_002047"
     CDS             2052..2912
                     /locus_tag="PXE44_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL5589522.1"
                     /translation="MRKHKLGIYEKALPKNISWQDRLSIAKVCGFDFVEMSIDETDER
                     LARLDWSKKERAKLVKTIINTGVTIPSMCLSGHRRFPFGSCDEATRQKAYEIMEKAIQ
                     LAVDLGIRTIQLAGYDVYYEEQDEGTIQRFQQGLEWAVELAASNQVTIAVEIMDTQFM
                     SSISRWKKWDEIIKSPWFTVYPDLGNLSAWNDNVAEELKLGIDKISAIHLKDTYKVTE
                     TCKGQFRDVPFGDGCVDFAACFKTLAELNYRGAFLIEMWTEKAEEPITEIINARRWIE
                     QKMQEGGFTL"
     gene            2909..3628
                     /locus_tag="PXE44_002048"
     CDS             2909..3628
                     /locus_tag="PXE44_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL5589523.1"
                     /translation="MTQLTELTPALQALRERVLKANLELPKYGLVTFTWGNVSEIDRE
                     TGLVAIKPSGVEYDAMTVEDIVLVDLQGNRVYGDKKPSSDTETHLELYRQFTDIGGVV
                     HTHSRNAVGWAQAGRDLLALGTTQGDYFYGAIPCTRKMTAEEIAGSYELETGKVIVET
                     FRTRGIEPKAVPGVLVHSHGPFTWGKDGHDAVHNSVVLEEVAYMNFISHQIRPQIGPM
                     QQELLDKHYLRKHGANAYYGQ"
     gene            3849..6326
                     /locus_tag="PXE44_002049"
     CDS             3849..6326
                     /locus_tag="PXE44_002049"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012311.1,HMM:TIGR02601.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL5589524.1"
                     /translation="MYLLEGNQKVKNRNYFVAIATILALSPEVNATELSDQLASRTRY
                     YPSATDSYYSYYKYAPIYSGYYTSNSRNGYSSWYYPGSNAGVTTRTVSTTVNGITVTK
                     VYTTHNGRTTVRTTYAPAVNKPIVNKPTPIANKPVVDTPTQPVIDANPIMDPPVQPIV
                     DNKPITDTQPNPITDNKPVIESPIDDTRPVIIRPTTPVNLHPEPVAVGVPNVGIRWND
                     RSRGYNIDDPNNKQNVKLTGEGVKVGILDSGFKNYLMQDDILKKFGNRAVIIETGRTA
                     PASATHGIEVAEMVGGGNSRNGVASKSTLLLADITKTTNAGTGLSATAPIYYDLWNRG
                     ARIFNQSYGIPKPLTYFNNNRNSSYYYLHQFDGNVLQFYKDKVKEGGLFVWAAGNNRG
                     DLDPSLQAGLPHYEADLEKGWISVVAVATRTNQGLGNFEWSNLLPYSQAGVAKNWTVT
                     AMGDYIFNLGGRNIIASGSSFAAPAVTGTAALVKQKYPWMDGNLIKQSILSTATDIGA
                     PGVDDVYGWGLLNIEKATNGPARFGKRLALADNVTVNIPNGSYEFSNNINGDAGLVKN
                     GQGELILSGASTFEGDTTLNEGAVTINGKKYVSKVNVSPSGTLVVNNTTLENGVNNEG
                     TVVNQGYSTIKRAYVASAQSTLVSDLGSNLNVKGEVDLNGSTLSLAAVKEGKAQYVTQ
                     KGLSMSAITSDSAIKGNFAAIETSGLLTATAEKTNENEVKATVSRKNVATALESSFSA
                     LDNQIEGNNGSTNATNDEFAQQAATLQNSIANINTQSVVLDSLSGQIYASAQALTFEN
                     SETVNKDLSNRLVMLGTLDNVGETAGV"
     gene            6336..7151
                     /locus_tag="PXE44_002050"
     CDS             6336..7151
                     /locus_tag="PXE44_002050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5589525.1"
                     /translation="MYGNGTLKEIGFGEGKTNTYAGQIGFDKPVTDHVILGAALNYSK
                     AKVNFDRYGGSSKADGIGASLYARIGNKHKTPWYLQGRAGYGSVDSDVERHIILADNN
                     ASTAKINHRDRVFSAYVESGYDFKQGRFALTPFVGFSHDVVRRGAFSEQNSQFGLTAD
                     KATYKQTSGLIGLRTSLEVNLAGMKTTFQGYVNHQKAFNREDLSFKASYTGLQDAKFD
                     VKGIGLAKHKTWVGIGALAEVNKNTSLYVNYDLKLAKNSGHNNVVTAGIRINF"
BASE COUNT         2239 a         1339 c         1624 g         1975 t
ORIGIN      
        1 gtacaaattt ttgtgattgt ttatgattgg ttttgattgc attgttctta ttttagcgta
       61 aatcaggaga ctattatgag caaaattcaa gaaatcactc gtgaaagctg gatcctttca
      121 actttccctg aatggggtac ttggttgaat gaagaaattg aagaagaagt tgtaccggaa
      181 ggcaattttg caatgtggtg gctaggctgt gtcggtgtgt ggattaaaac cccagctggt
      241 gcgaatatct gtatggattt atggtgcggt cgtggtaaat ccaccaagaa agtaaaagat
      301 atggtgcgtg gtcatcaaat ggcaaatatg gcaggggtgc gtaagctcca gcctaatttg
      361 cgggctcaac cgatggtgtt agatccgttt gcgattaacg aagtggactt cattctcgct
      421 tctcactatc actcagacca tattgatgta aacgtggcag cggctatttg caataacccg
      481 aaattagacc acgttaaatt tgtcggccct tggcactgtg cagaattatg gaaaaaatgg
      541 ggcgtgccgg aagaacgtat tattgtggta aaaccgggcg atgtcgtgaa attaaaagat
      601 gtagaaattc acgccctaga ttcatttgac cgtacctgtt tagtgacctt accggtagaa
      661 ggtgcagaag aacgtggcgg agaacttgca ggcttatgcc catcagatga agaaatggga
      721 cgcaaagcgg tgaactatgt gttcaaaacc ccgggcggta cgatttatca cggtgcagat
      781 tctcactact caatccaatt cgctaaacac ggtaagcaat ttgatattga cgtagcgctg
      841 aataactatg gcgaaaaccc ggttggtatt gcggataaaa tgacttcagt tgatttatta
      901 cgtatggcgg agtgtttacg ctgtaaagtg attattccgg ttcaccatga tatttggaca
      961 aactttatgg caagtacgca agaaattatt gatttatggc gtatgcgtaa agatcgctta
     1021 caataccaat tccacccatt tatttgggaa gtgggcggca aatatgttta cccacgtgat
     1081 aaagacttaa ttgaatatca tcaccctcgc ggctttgatg actgcttcga gcaagagcca
     1141 aatattcaat ttaaatccat gctataggtg agcagtcgcc actctcactg gagagtggcg
     1201 ttttagcaag ttcattatgg agcagtagtg attagtaatg ggataaagta gtgaacgaaa
     1261 gttatagaca caaagagtta ttagccctgt tggaagagcg gaaagcactg tcaacagcag
     1321 atattatgca gctgttaaat atttctccgg caacagcaag acgtgatatc agtaaattag
     1381 atggtcaagg taaattaaaa aaggttagaa acggagcaga agcaattctg gctcctacat
     1441 ttttacagca cacaaaaata actaacaatg cgcaggagaa acgtagaata gcggaagctg
     1501 ccggtcgttt atgtaaagat ggggaaagtc ttattctgac gtgtggttcc acaatggaaa
     1561 tgctcggtga agtattatgt ggtagaaaat tgcaaattat taccaattat ctaccgcttg
     1621 caaaccagtt gattgcaggt tgccacgatg atgtagtgat tatgggtggg caatataata
     1681 aacataaaaa agtgatgctc tcaattaaca cccaaaatga gatgacttat gccgctacca
     1741 caatgtttac cagtggcaaa ggcttaacca gcgaaggatt atttaaaacc gatatgatta
     1801 ttgccaatgc cgagcaacaa atggcaaagc acgcagaacg ttatgtggtg ttattggata
     1861 gcaccaagct cggcaaaaaa gtcggtatgc tgtttaaaga gttcgataaa attgatttat
     1921 tgattaccgg caaagaggcg gatccgacta ttatccaagc attaagaaac aaaggcctgg
     1981 atattatctt ggcttagcct gtagcggtca aatttaatca aaattttact tatcatcttc
     2041 aagggaaaac tatgagaaaa cataaactgg gtatttacga aaaggccttg ccgaaaaaca
     2101 tttcgtggca agaccgcctt tctatcgcca aagtctgcgg ttttgacttt gtggaaatgt
     2161 cgattgacga aacggacgaa cgcttagccc gccttgattg gagcaaaaaa gaacgtgcca
     2221 agctggtgaa aaccatcatc aacaccggcg tgaccattcc gtcaatgtgc ttgtccggtc
     2281 atcgccgttt cccgtttggt tcttgtgatg aagcgacccg ccaaaaagcc tatgaaatta
     2341 tggaaaaagc cattcaactg gcggtcgatc tcggtattcg caccattcag cttgccggct
     2401 atgacgtcta ttacgaagag caggacgaag gcacaatcca acgcttccaa caaggcttag
     2461 aatgggcggt ggagctggcg gcaagcaacc aagttaccat tgcggtggaa attatggaca
     2521 cccaatttat gagttcgatt tcccgctgga aaaaatggga cgagatcatc aaatcaccgt
     2581 ggttcaccgt gtatccagat ttaggcaacc tctcagcgtg gaacgacaac gtagcggaag
     2641 agctgaaact cggcatcgac aaaatctctg ccattcactt aaaagacact tacaaagtga
     2701 ctgaaacctg caaagggcag ttccgtgatg tgccatttgg tgacggttgc gtagatttcg
     2761 ccgcttgttt taaaaccctt gccgagctga attaccgtgg cgcattctta attgaaatgt
     2821 ggacggaaaa agccgaagag ccgattactg aaatcatcaa cgcccgccgt tggatcgaac
     2881 aaaaaatgca agaaggagga tttaccctat gacgcaatta actgaattaa cccctgcatt
     2941 acaagcctta agagagcgtg tgttaaaagc caatttagaa ttaccgaagt atggcttggt
     3001 aactttcact tggggtaacg taagtgaaat cgaccgtgag accggtttag tcgccatcaa
     3061 accttccggc gtagaatacg atgcgatgac ggtggaagac attgtattgg ttgatttaca
     3121 aggtaatcga gtttacggag ataaaaaacc gtcttctgat acagaaacgc acttagaact
     3181 ttatcgccaa ttcactgata ttggcggtgt ggtacacact cactctcgca atgctgtagg
     3241 ttgggcacaa gcagggcgtg atttattggc acttggcacc actcaaggcg attatttcta
     3301 cggtgcgatt ccttgtactc gcaaaatgac cgcagaggaa attgccggca gttatgagct
     3361 ggaaaccgga aaggtgattg tcgaaacttt ccgaactcga ggtattgaac cgaaagcagt
     3421 tccaggtgtg ttggtacatt cacacggtcc atttacctgg ggaaaagatg gacacgatgc
     3481 tgtgcataat tctgtggtat tagaagaggt ggcttacatg aattttatta gccatcaaat
     3541 tcgcccacaa attggcccaa tgcaacagga attattagat aagcattatc tgcgtaagca
     3601 tggtgcaaat gcttattatg ggcaatagct aataacctaa aaattatttt ccaaattatc
     3661 aacagctttc gaggggcttt ctataatcgg aatagctcct cagtctgttt gagtttatgg
     3721 atagatccac aaataattac cattttcctt ttcttgattt taattttgat ttttgttcta
     3781 atctctgcct cttgttttta caagtctatt aaggatccgt agtagatttg aaataaacaa
     3841 gagtatcttt gtatttatta gaaggaaacc aaaaggtgaa aaatagaaat tattttgttg
     3901 caatagcaac aatattagcc ttatcgccag aggttaacgc aactgaactt tcagatcagc
     3961 ttgcaagtag aacaagatat tatccttccg ccacggatag ttattatagt tactataaat
     4021 atgcaccgat ttattcgggc tactatacat cgaatagccg taatggttat tcgtcgtggt
     4081 attatccagg tagcaatgca ggtgtaacaa ccagaactgt ttcgacaaca gttaatggca
     4141 ttacagttac aaaagtttat acaacgcata atgggagaac aaccgttcgt accacttatg
     4201 ctcctgctgt gaacaaacca attgtaaata aaccgacacc gattgccaat aaacccgttg
     4261 tagatacgcc aactcaaccg gttattgatg ccaacccaat tatggatccg ccagtccaac
     4321 caatagttga taataaaccc atcactgata cacagcctaa tccaattaca gataacaaac
     4381 ctgtcattga atcgccaatt gatgacaccc gtccagttat cattaggcct acaacaccgg
     4441 taaatcttca tcctgaacct gtagctgttg gtgtacccaa tgtaggtatt cgatggaatg
     4501 accgctctcg tggttacaac attgatgatc ctaataacaa acaaaatgtt aagttaactg
     4561 gtgaaggtgt taaagtcggt atcttagata gtggttttaa aaactattta atgcaggacg
     4621 atatactcaa aaaatttggt aatcgcgctg ttattattga aacagggcgt actgccccgg
     4681 cttctgcaac acatggtatt gaggtcgcag aaatggttgg gggagggaac tctcgcaatg
     4741 gagtagcgtc taagtcaacg ttattacttg cggatattac caaaacaaca aatgcgggta
     4801 caggattatc cgcaacagct ccaatttatt atgacttatg gaatagggga gcccgtattt
     4861 ttaaccaatc atacggtatt ccaaagcctc ttacttattt taataacaat agaaattcta
     4921 gctattacta tttacaccaa tttgatggta atgtgttgca attctataag gataaagtaa
     4981 aagagggggg cttatttgtc tgggctgcgg ggaataaccg tggtgatctt gatccatcac
     5041 tccaagctgg gttacctcat tatgaagctg acttggaaaa aggttggatt tctgttgttg
     5101 cggtagcaac cagaactaat cagggattgg gtaactttga atggagcaat ttattgccat
     5161 attctcaagc cggtgtggca aaaaattgga ccgttaccgc aatgggtgac tacatcttta
     5221 atttaggagg tagaaacatt attgctagtg gttcatcctt tgcagctcca gccgttacgg
     5281 gtacagcagc tctcgtgaaa caaaaatatc cttggatgga tggtaacctt atcaaacaat
     5341 ctattctctc gacggcaacc gatatcggcg ctcctggagt tgatgatgta tatggatggg
     5401 gtttacttaa tattgaaaaa gccactaatg gccctgcgcg ttttggtaaa cgactcgctt
     5461 tagccgataa cgtgactgta aatattccaa atggaagcta tgagttttcc aataatatta
     5521 acggtgatgc aggcttggtg aaaaatggcc aaggcgaatt aattttatcg ggtgctagta
     5581 cttttgaagg ggatacaaca ctcaatgaag gtgctgtcac tattaacggt aaaaaatatg
     5641 tgtctaaagt aaacgtatct ccaagtggta cattggttgt caataatacc acgttagaaa
     5701 atggcgtaaa taacgagggt acagttgtca accagggtta ttctacgatt aagagagctt
     5761 acgttgcttc agcacaaagt acgctagtgt cagatttagg ttcaaactta aatgtaaaag
     5821 gagaagttga cttaaacggc tctactttat cactagctgc ggtaaaagag ggtaaagctc
     5881 agtatgtgac ccaaaaaggt ttatcaatga gtgctattac ttctgattcc gcaattaagg
     5941 gtaactttgc tgcaatagaa acctcagggt tattaactgc gacggcggaa aaaaccaatg
     6001 agaatgaagt aaaagcaaca gttagccgga aaaatgtcgc gactgcttta gagagctcat
     6061 tttcggcatt ggataaccaa attgaaggaa ataatggttc tacaaatgct acaaatgatg
     6121 aattcgcaca acaagcggct actttacaaa acagtattgc aaacatcaat acgcaaagtg
     6181 tggttttaga tagcttatct ggtcaaattt atgcgtctgc gcaagcgctt acttttgaaa
     6241 actcagaaac agtgaataaa gatctctcta accgtcttgt aatgttagga acgctggata
     6301 atgtaggtga gactgctggt gtgtgagtaa cgggtattta tgggaatggt actctaaaag
     6361 aaatagggtt tggtgaaggt aaaaccaata cctatgctgg ccaaataggt tttgataagc
     6421 cagttacaga ccatgtgatt ttaggtgcgg cacttaatta ttctaaagca aaagtgaact
     6481 tcgatcgtta tggcggtagt tcaaaagcag atggaatagg tgcatcatta tatgcccgta
     6541 taggcaataa gcataaaaca ccttggtatt tacaaggtcg agccggttat ggcagtgttg
     6601 attcagatgt agagcgtcat attattttgg cggataataa cgcaagtaca gcaaaaatca
     6661 accaccgaga cagagtattt tctgcttatg ttgaatcagg ctatgacttc aagcaaggtc
     6721 gctttgcgtt aactccattt gtcggtttta gtcatgatgt tgtcagacgt ggcgcattca
     6781 gtgagcaaaa cagtcaattt ggtctcaccg cggacaaagc aacctataaa caaacaagcg
     6841 gattgattgg tttacgaaca tctttagagg tgaatttagc tgggatgaaa acgactttcc
     6901 aaggctatgt aaatcatcaa aaagcgttta atcgagaaga tcttagcttt aaagcaagtt
     6961 atacaggttt acaagatgca aaattcgacg taaaaggtat tgggcttgca aaacataaaa
     7021 cgtgggtagg gattggtgca ttggcggaag taaataaaaa tacttcctta tatgtgaatt
     7081 acgaccttaa actcgcaaaa aatagtggtc acaacaacgt tgtgactgct gggattagaa
     7141 ttaatttcta actgaatctt tttaaacaaa acgggcg
//
LOCUS       DANLZC010000125         7145 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.125, whole genome shotgun sequence.
ACCESSION   DANLZC010000125 DANLZC010000000
VERSION     DANLZC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.125"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            32..838
                     /locus_tag="PXE44_002051"
     CDS             32..838
                     /locus_tag="PXE44_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589526.1"
                     /translation="MNIKDLLKEISFKAVINDLNFSSCLYIKPNTSKLYVILNGALIK
                     EPRNPLIYHRHSWHLEFDGSVLYIADPTLFKYESINLAWYVGDKDTDLYPYLAEFVTS
                     VATHLNVETDHIVTYGSSGGGFAALQLGARIGNGVVTVCINPQTNIMKYVPSHRNAFL
                     NACFDFNAEEYDSLSNQEKFNALVLLKENTSKILYIQNTQDEFHHKWHFLPFLELKGI
                     DLSEIEKTHWREQQSKDVKVILYSHESGHAAEPKEMIPEILSAVDQLIAS"
     gene            840..1835
                     /locus_tag="PXE44_002052"
     CDS             840..1835
                     /locus_tag="PXE44_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5589527.1"
                     /translation="MLFYLVSDSLTRLSLSNESLVDLGRITYIGNYPFLHNAKNAKFL
                     MVESAWQGYKNRWKYKIASYPDNPERTNEKLVRLVEKVKDKGIPTVFWNKEDSVHFDR
                     FIDSAKHFDHIFTVDQNAIARYREVVPATTTVNVGMFPVQPKIHNFQGFNFKHLAANF
                     VGSYSHHIHNKRRERQDMLFGAALKAGLPVTIFDRNSDRKSENYRYPKNSFNLNVQPN
                     VSYTETANIYRDYLVSLNINTVEDSPTMFSRRVVEILACGGILVSTPSMAIDNIFKEY
                     CHIVQSEDEAIELFQRLKRGASRDDLNKAIAGSEFVLSNFTWEHFLSQIQKVVYR"
     gene            1954..3138
                     /locus_tag="PXE44_002053"
     CDS             1954..3138
                     /locus_tag="PXE44_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDL5589528.1"
                     /translation="MKQKKLNTTLLFITLSLISACSSLPTSGPSYSEILASNENTAET
                     QLPEVNLIKLDNITVQNLYQEQQSQRFSGFDGSMGVGGYAGTVNVGDILEISIWEAPP
                     AVLFGGTFSAEGQGNGHLTQLPQQMVNKNGTVTVPFIGNIKVAGKTPEVIQNQIVGAL
                     SRKANQPQALVRIANNLSSDVTVIRQGNSIRMPLSANNERILDAVAAVGGTSENIEDV
                     TVRLTRGNKVRSMAFETLIADPSQNITLRSGDVIALMNTPYSFTGLGAVGNNQQMRFS
                     SKGITLAEAIGKMGGLIDERSDPRGVFVFRHIPFTQLDYDNQAQWREKGYAEGMDVPT
                     VYQVNLLKPQSMFLLQRFPIQDKDIVYVSNAPLSEFQKFLRMIFSITTPITGTANTIR
                     AY"
     gene            3210..4313
                     /locus_tag="PXE44_002054"
     CDS             3210..4313
                     /locus_tag="PXE44_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL5589529.1"
                     /translation="MSKKKKHFLANPLLWLTVIIPTACSGIYYSLFASDIYVSESSFV
                     VRSPNNQSSLTGVGALLQNSGFARSQDDTYSVQEYMRSRTALEQLEAEIPVKRFYSEQ
                     GDLLSKFNGLGFNDTQEAFYRYFKERLNIDVDSISGIATLRVRAFNAEEGQKINENLL
                     QQGETLINRLNERARQDTVEFAEERVKEAEKDVNNTALALSQYRTKNKIYDLPAQSSV
                     QLSLISTLKSELIRVETQLAQLKSITPDNPQVAALQMRQKSLKKEIDDQSKQLAGGNT
                     SVATQTAEYQRLVLANELAQQQLAAAMTSLQNTRGEADRQQLYLEVIGRPSKPDWALE
                     PYRIYNIISTFFIGLILFGVLSLLLASIREHKN"
     gene            4313..5110
                     /locus_tag="PXE44_002055"
     CDS             4313..5110
                     /locus_tag="PXE44_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5589530.1"
                     /translation="MRYGDNTTFKRSCVIQKRVIHALLMRELITRYGRKNIGFLWLFI
                     EPLLLTLVITVAWKYIKADKFSTLNIIAFMITGYPIAMMWRNASNRAQGAISANLSLL
                     YHRNVRVLDTIFTRVLLEVTGASIAQILLMTIFIAIGWIEMPNDIFYMLCAWILMAFF
                     ALGLGLIICTLAHYIEPFGKIFGTFSFFLLPLSGAFFFVHNLPLTAQNIALWFPMIHG
                     TEMFRHGYFGETVITYESISYLIVSDIILLFIGLLCVRSFSKGIEPR"
     gene            5107..5754
                     /locus_tag="PXE44_002056"
     CDS             5107..5754
                     /locus_tag="PXE44_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5589531.1"
                     /translation="MISIRNVSKQYRTNNGWKTILKDINFELKKGEKVGILGRNGAGK
                     STLIRLMSGVEPPTTGTIERQMSISWPLAFSGAFQGSLTGMDNLRFVSRIYDADFDYV
                     KSFTEEFSELGEYLYEPVKSYSSGMKARLAFALSLSIEFDCYLIDEVIAVGDARFAAK
                     CKHELFEKRKDRSLILVSHSPASIKEYCDSAMVLEEGIMHTFDSIDNAYNFYNKT"
     gene            complement(5832..6461)
                     /locus_tag="PXE44_002057"
     CDS             complement(5832..6461)
                     /locus_tag="PXE44_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDL5589532.1"
                     /translation="MDFSKILSQVLDTAKDAATNGLIKGNSKNDQIAKIGGGAVAIGL
                     ISMLFGRNGGSSLAKLGSLAAIGSLAYQAYQKYQAQNSSVSELSQNNFENVDENVASK
                     VILQAMIAAAASDGAITEEEKAAIVSQVGDEAEVIEWIRQEMDTPATVEEIARQVGND
                     QALATQVYLASRAVCRDLERKEIVFLANLADALGLAEALVEQLEKDAGF"
     gene            complement(6533..7006)
                     /locus_tag="PXE44_002058"
     CDS             complement(6533..7006)
                     /locus_tag="PXE44_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDL5589533.1"
                     /translation="MLEKITACPCQTGKRYAECCQPFHLKQTFASTAEQLMRSRYSAY
                     TLVNIPYIVETTVPSQQKLLDQTAMKEWAENTKWVGLSVLNHLPSLSRIHSQVEFKAF
                     FDTDEGIQGHHETSLFVQIDERWFFVDPTVPLPHQKQPCVCGSGKKFKHCCGNYL"
BASE COUNT         2312 a         1225 c         1319 g         2289 t
ORIGIN      
        1 ataaagctca atacaactag gagtaaactt tatgaatatt aaagacttac ttaaagagat
       61 cagctttaaa gctgtaatta atgatcttaa tttttcatcg tgtctatata tcaaacctaa
      121 tacatcaaaa ttatatgtga tattaaatgg tgcattaatc aaagaaccca gaaatccctt
      181 aatttatcat agacatagtt ggcatctaga atttgatggc tcagttctat atatcgcaga
      241 tccaacatta tttaaatatg aatcaattaa tttggcttgg tatgttggag ataaagatac
      301 tgatttatat ccatacttag ctgaatttgt aacttctgtt gctacacatt tgaatgttga
      361 gacagaccat attgttactt atggttcaag tggtggtgga tttgctgcat tacagttggg
      421 agcaagaatt ggaaacggtg tagttaccgt ttgtattaat ccacaaacca acatcatgaa
      481 atatgtacca agtcatcgta atgccttttt aaacgcttgc tttgacttta atgcggaaga
      541 gtacgatagt ctttctaatc aagagaaatt taatgcttta gtattactta aggaaaatac
      601 atcaaagatt ttgtatattc aaaatactca ggatgaattt catcataaat ggcatttcct
      661 cccattccta gagttaaagg ggatagatct atcggaaata gaaaagaccc attggagaga
      721 acaacagagc aaagatgtaa aagttatttt gtattctcat gaatcaggtc atgcagctga
      781 acctaaggaa atgattcccg agatattatc cgctgttgat caactgatag ctagctaaaa
      841 tgctgtttta tttggtatct gattctttaa ctaggctttc attatcaaat gaaagcctag
      901 ttgatcttgg aagaataaca tatataggaa attatccttt cttgcataat gcaaagaatg
      961 ctaaatttct tatggtcgaa tccgcctggc aaggctacaa aaaccgttgg aaatataaaa
     1021 ttgcctctta tcctgataat cctgaacgaa ccaatgaaaa gttggttcgt ttagtagaaa
     1081 aagtgaagga taaaggcatt ccaacggtct tttggaacaa agaagattct gtgcattttg
     1141 atcgatttat tgattcggcg aagcattttg atcatatttt taccgtagat caaaatgcca
     1201 ttgctcgtta tagggaagta gtgcctgcga caacaaccgt caatgtcgga atgttccctg
     1261 ttcagcccaa aatccataac tttcaaggat ttaattttaa acaccttgcc gcaaattttg
     1321 tagggagtta cagtcatcat atccacaata agcgtagaga aagacaggat atgttgtttg
     1381 gtgcagcact taaagcaggt cttccggtaa ccatctttga tagaaactct gatagaaaat
     1441 cggaaaatta tcgttaccca aaaaatagct ttaatttaaa tgttcaacct aatgttagtt
     1501 atactgaaac tgctaatatt tatcgtgatt atcttgtttc tttgaatatt aatactgtcg
     1561 aagattcccc tacgatgttt tctcgcagag tcgttgaaat attagcttgt ggaggaatat
     1621 tagtctctac accaagtatg gctatcgaca atatatttaa agaatattgt catatcgtac
     1681 aaagtgaaga tgaggctata gaattattcc aacgtttaaa acgaggtgct tctcgtgatg
     1741 atttgaataa agcgattgca ggctctgaat ttgtattgag taactttact tgggaacatt
     1801 tcttatccca aatacagaaa gttgtatata ggtaacttaa atagcattat taaaaactct
     1861 ctgccaagaa attattttct atttcgagta attaattagg ggaaatttag tctagttatt
     1921 taggatgaat ccaaattaat tataggtact ataatgaaac aaaaaaaatt aaatactaca
     1981 ttattattta taacattatc attaatttca gcctgctcta gcctaccaac gtccggccca
     2041 agctatagcg aaatcttagc aagtaatgaa aatacagctg aaacgcagtt gcctgaagtc
     2101 aatttaatca agttagataa catcaccgta caaaacttat accaagagca acaatctcaa
     2161 cgtttttcag gatttgatgg ctcaatgggt gtgggtggtt atgctggaac agttaatgta
     2221 ggcgatatat tagaaatatc catttgggaa gcgccacctg ccgtgctatt tggtggaaca
     2281 tttagtgctg aagggcaagg taatgggcat ttaacccaat tacctcagca aatggtgaac
     2341 aaaaatggaa cggtaactgt gccttttatc ggtaatatta aagttgctgg aaaaacacct
     2401 gaagtaattc agaatcaaat tgtcggagca ttgagccgta aagctaatca acctcaagca
     2461 ttagttcgta ttgcgaataa cttgtcgtct gatgttacgg ttatccgcca aggaaatagc
     2521 attcgtatgc cattaagtgc caataatgag cgtattttag atgcagttgc ggcagtaggt
     2581 ggtacaagcg aaaatattga agatgtaaca gttcgtttaa cacgtggaaa taaagtgcgt
     2641 tcaatggcgt ttgaaacatt aattgctgac ccctcacaaa atattacatt acgttcaggt
     2701 gatgttattg ctttaatgaa tactccttat agctttaccg gattaggagc agtaggtaat
     2761 aatcaacaaa tgcgtttttc aagtaaaggc attacccttg ctgaagctat cggtaaaatg
     2821 ggaggattaa ttgatgagcg ttcagaccct cgtggtgtgt tcgtattccg ccatattcct
     2881 tttacacaat tagattacga taaccaagct caatggcgtg aaaaaggtta tgcagaggga
     2941 atggacgttc caactgttta tcaggtgaat ttattaaaac cgcaatcaat gtttttattg
     3001 cagcgtttcc ctattcaaga taaagatatt gtatatgtgt cgaatgctcc actgtctgaa
     3061 tttcaaaaat tcttgagaat gattttctcc attactacac caattacagg tacagctaat
     3121 acaattagag cgtactaatt agctcataga taaaatggta aaaaatcatt aaaaaatgac
     3181 cgcttgtaac acttttcgtt gaggatttta tgtcaaagaa gaaaaaacat tttttagcca
     3241 accctttgtt gtggctaact gtaattattc caactgcttg ttctggaatt tattattcct
     3301 tatttgcctc tgatatttat gtttcagaat ctagctttgt ggttcgttcg cccaataatc
     3361 aatcgagctt aacaggcgtt ggtgctttat tgcaaaactc cggtttcgcc cgttctcaag
     3421 atgataccta ttcagttcag gaatatatgc gttcccgtac cgcattagaa caacttgaag
     3481 cagaaattcc ggtaaagcgt ttttatagtg agcaaggtga tttactaagc aaatttaacg
     3541 gtttaggatt taacgatacc caagaagcat tttatcgtta ttttaaagaa cgtctgaata
     3601 ttgatgtcga ttctatttca ggtattgcaa cgctacgcgt tcgtgctttt aatgcagaag
     3661 aaggacaaaa aattaacgag aatttattac agcaagggga aacattaatt aaccgcctta
     3721 atgaaagagc aagacaagat acggttgagt ttgcagaaga gcgtgtaaaa gaggccgaaa
     3781 aagacgtcaa taatactgcg ttagcattaa gccaatatcg aactaaaaat aaaatttatg
     3841 atttaccggc acaatccagc gttcagctct cattaatttc taccttaaaa agtgaattaa
     3901 ttcgggtaga aacacaatta gcacaattga agtcaattac gccggataat cctcaggtag
     3961 ccgcattaca aatgcgtcaa aaaagtttga aaaaagaaat tgatgatcag tccaaacaac
     4021 ttgcaggagg taatacttct gtagcaacgc aaactgctga atatcaacgt ttggttcttg
     4081 ctaacgaatt agctcagcaa caacttgcag cagcaatgac ttcattacaa aatacacgag
     4141 gtgaagctga tcgccaacaa ctttatttag aggtaattgg taggccaagc aaaccagatt
     4201 gggcattaga accctacaga atttataata ttatatcaac tttttttatc ggcttaattc
     4261 tatttggagt attaagtttg ttactagcaa gtattagaga gcataaaaac taatgcgata
     4321 tggtgataat acaaccttta aacgttcctg tgtgattcaa aaacgggtga ttcacgccct
     4381 tttaatgagg gaattaatta ctcgttatgg gcgtaaaaat attggcttct tatggctttt
     4441 tatcgaacca ttattgttaa cgttagtgat tactgttgct tggaaatata taaaagcaga
     4501 taagttttca actttaaata ttatcgcttt tatgattaca ggctacccta tcgcaatgat
     4561 gtggagaaat gcctctaata gagctcaagg agcaatatca gcaaacttaa gtttgcttta
     4621 ccacagaaat gtgagagtgc tagatacgat atttactcga gttttattag aagttactgg
     4681 cgcatctatc gcccaaattc tcttaatgac tatatttatc gctattggtt ggatagaaat
     4741 gcctaacgat attttctata tgctctgtgc ttggatatta atggcatttt ttgccttagg
     4801 tttaggttta attatctgta ctttagcaca ttatattgaa ccttttggta agatttttgg
     4861 aacattcagc ttctttctac ttcctctttc tggtgcattc ttctttgtcc ataatttacc
     4921 gctaactgct caaaatattg ccttatggtt tccaatgatt catggtacag aaatgtttag
     4981 acatggttat tttggtgaaa cggtaattac ctatgagagc attagttatc ttatagtaag
     5041 tgatattatt cttctattta taggactgct ttgtgtgaga agttttagta aagggataga
     5101 accaagatga ttagtattag aaatgtaagt aagcaatatc gaactaataa cggttggaaa
     5161 accatattaa aagatataaa ttttgagctg aaaaaaggcg aaaaagtggg gattttaggg
     5221 cgcaatggtg caggaaaatc aaccttaatc cgtttaatga gcggagtaga acctccaaca
     5281 acaggtacga ttgaacgtca aatgtcgatt tcttggccac tggcatttag tggtgcattc
     5341 cagggtagtt tgaccggaat ggataatctt cgtttcgtca gccgaattta tgatgctgat
     5401 tttgactatg taaaatcttt caccgaagaa ttttcagaac tcggtgaata tttatatgaa
     5461 ccggtaaaaa gctattcttc tggtatgaag gcccgtttag cctttgcatt atcgctttct
     5521 attgaatttg attgttactt aattgatgaa gtgattgcag taggcgatgc tcgattcgct
     5581 gccaaatgta agcacgagct ttttgaaaaa cggaaagacc gttcattgat tttagtttct
     5641 catagcccag cctcaattaa agaatattgc gattctgcaa tggttttaga agagggtatc
     5701 atgcatactt ttgattctat agataacgct tataatttct acaataaaac ataaaagaac
     5761 aagcggttat atttcacaag tttttcgcaa aaaactctca aaatataacc gcttgttact
     5821 aaaactgaaa attagaaacc tgcgtctttt tctaattgtt caactaatgc ctcagctaaa
     5881 cctaaagcat cagctaaatt agctaagaaa acgatttctt tgcgttctaa atctctgcat
     5941 actgcacggg aagctaagta aacttgtgta gccaatgctt ggtcattacc tacttggcgg
     6001 gcaatctctt caactgtagc cggagtgtcc atttcttgac gaatccactc aatgacttca
     6061 gcctcatctc caacttggga aacaatagct gctttttcct cttcagtaat agcaccatct
     6121 gaagcagcag cggcaatcat tgcttgtaaa atcactttac ttgccacatt ttcatctaca
     6181 ttttcaaaat tattttggct aagttctgaa accgatgaat tttgcgcttg atacttttgg
     6241 tatgcttgat aagcaaggct accgatagcg gctaaagagc ctaattttgc taaacttgaa
     6301 ccgccattac gcccaaataa cattgaaatt aaaccaatag cgacagcgcc gccaccaatt
     6361 ttagcgattt gatcgttttt agaattacct ttaattaatc cgtttgtagc ggcatctttt
     6421 gctgtatcta atacttgact taaaatctta ctgaaatcca tttttgttcc tcttggtaag
     6481 taaatgaaat ttactcatta gtccttagat tcaaacaaga gttcaattca aattaaagat
     6541 aatttccaca acagtgctta aacttctttc cggagccaca tacacaaggt tgtttttgat
     6601 gaggcaaagg tacggtagga tccacaaaaa accatcgctc atcaatttga acaaaaagag
     6661 aggtttcgtg gtgtccctgt atcccctcat ctgtatcaaa aaaggcttta aattcaacct
     6721 gactatgtat tctcgaaaga gaaggtaagt gatttagcac ggataatcct acccattttg
     6781 tattttcagc ccactctttc attgcagtct gatcaagtaa tttctgctga cttggcactg
     6841 tcgtttccac aatataggga atattaacca aagtataagc actgtaacgg gagcgcatta
     6901 attgctctgc cgttgatgca aatgtctgtt ttaaatggaa aggttggcaa cattcagcgt
     6961 agcgtttgcc tgtttggcaa ggacaagcgg tgattttttc taacattttt acatttccgc
     7021 ataaaaataa agatgtgaaa agtttacgct ataaataaag aaaaataaat tttataaaaa
     7081 agcgtgacat accccttgtg gataacttat gcaaaaatcg tgaatttttt gggtattcat
     7141 tgctt
//
LOCUS       DANLZC010000126         7114 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.126, whole genome shotgun sequence.
ACCESSION   DANLZC010000126 DANLZC010000000
VERSION     DANLZC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.126"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            23..883
                     /gene="araC"
                     /locus_tag="PXE44_002059"
     CDS             23..883
                     /gene="araC"
                     /locus_tag="PXE44_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDL5589534.1"
                     /translation="MYKNLSLLSPTYPFDLEFIAGLTQCEKGNYLDSIVDRQQGMHGY
                     MLQLTTFGRGSVFDGKHVFTAHRGQLLLFTPNAVQHYHRHPESQYWHYKWVYFQPNPK
                     WNKWLNWSDMEEKIGRITISDDRYFQEISQLFSKIELELKSSKFFKEDIATSLLEYLL
                     MKCISAEKIEVIPVIDQRILTVCDLILTNLAKNESLEAMAEKVFLSPSRLSHLFKQSL
                     GISLVQWRELQRISESKKLLYFSNISISNIAKSLGYEDSLYFSKIFKKHTGLSPSEFR
                     TLERRKDSEM"
     gene            1070..2044
                     /locus_tag="PXE44_002060"
     CDS             1070..2044
                     /locus_tag="PXE44_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5589535.1"
                     /translation="MKKPTFKHALFSSILALACYGSAFANDVVNISKIDGMPWFNRMA
                     EGVVQAGKDNNINAYQVGPSNTDAPQQVKLIEDLIAKKVSAISIVPNDANALEPVLKK
                     AKQNGIVVLTNESVGQPSADWDIEIIDNAQFAADYVEEVAKAMGGKGGYVIYVGGLTV
                     PQHNLWADLFVKYQKEHYPDMFEVTSRMPVAENINDARRTTIDLVKAHSDLKAVVSFG
                     SQGPIGAGLAVKEKRLKNKLNVFGMMIPSQAANLIKSGDITMGITYDPANAGYALAAV
                     AAKVLKGEKIEDGLEIPTVGKAKVDSEKKLLQFHNVLKVTKENIDKLY"
     gene            2099..3589
                     /locus_tag="PXE44_002061"
     CDS             2099..3589
                     /locus_tag="PXE44_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5589536.1"
                     /translation="MAFISMRHISKTFHGIKALNQVNLSLDYGEALCLAGQNGCGKST
                     LIKILSGVYQPDKGAEIQIGATQYTKLTPQESIDKGIQVIYQDLALFPNLTVAENIAI
                     NLHRKFGWVSQDHIHQVALTAIQSINANLDLNAILEDLPIAQQQLVAICRALAQNARL
                     LIMDEPTASLTAKEVKDLLDVVIKLKSKGISIIFVSHKLQEVMSVSDSVLVLKDGNMV
                     GQYPIREMNEKRLGFLMTGLEIDYKRLDLPDFLQRKTVLEVRNLTLPHQYQEINFSLR
                     EGEIVALTGLLGSGRTELCLSLFGITQPQSGDILLNGEKVIFNTNRDAIAKGIAYVSE
                     DRMHTGLIMAESIHHNIISTIFHKITDKLQIIQSSKAYASSQALIDSLKIKVSDSDLP
                     VNTLSGGNAQRVSIAKWLAIDPKIIILDAPTIGVDIANKEGIFQIIKSLAQQGIAVIF
                     VTDEVEETYYNSHRVIVMKKGRIVDEILPAYSSEKSIAEVIYENQQ"
     gene            3573..4532
                     /locus_tag="PXE44_002062"
     CDS             3573..4532
                     /locus_tag="PXE44_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5589537.1"
                     /translation="MKISNNHILSSTLLILILLLTLFTRDFATIENLYDVVNNYAMLM
                     ILACGLFVVLLSGGIDISFPAITIISQYVMVTLIGKYDVGFAGVFVISSTIGILLGFI
                     NALFVNRLSVPSIIITISTLNIFYGGLLYFTKGVWLYEYPEWFTQEIVFFKQITAEGV
                     EFGLSFQALMAILAVILTGLITNKLTIGRKIYALGGNPDATSRIGFNLLFLHLFAYGY
                     MGFMAGLAGIVQSYTVQSVAPDSLLGYELTVLAAVVLGGASLSGGRGTLLGTVMGVML
                     LAIIQNGLNLMNVSSYWQTIITGIIIILSISATALSQRKKQGA"
     gene            4535..5485
                     /locus_tag="PXE44_002063"
     CDS             4535..5485
                     /locus_tag="PXE44_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5589538.1"
                     /translation="MKSKLDPTIFYLLALFAVAVIAFSIFIPDIFWSVSNFQSMASQI
                     PVLGILTLAMALPMLTGGINLSIIAGMNACSLIIAYFITQYTGISWLFIAILLSLCCA
                     LAIGLLNGTLIAIIRVSPILATLGTMTLINGINILVTNGSTIANFPETMLSINASAFL
                     WIPLPILIFLFLAGLIWFFLEKTAMGKAVYFIGNNEKATYYSGINTKKVLIWVYVISS
                     ILCVFAAILMMSKLNSAKASYGDSYLLISILAAVLGGINPDGGKGKLLGIMLALVLLQ
                     VIESGLNMLGISSYITMILWGSLLLFFIFLQKNRLFRLTQ"
     gene            5498..7093
                     /locus_tag="PXE44_002064"
     CDS             5498..7093
                     /locus_tag="PXE44_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL5589539.1"
                     /translation="MQNEKQLIEQGNTVIGIELGSTRIKAVLISSDGTILATGGADWE
                     NRLIDGIWTYHQNEIWEKLQAAYMDLSKTVKEKYHTTIRTAKALGISAMMHGYLPFDK
                     HGNQLVAFRTWRNNITLTSSEKLTALFQYPIPQRWSIAHLHQALLNQEPHLPEIDYLT
                     TLSGYVHWQLTDHKVLGIGDASGMFPIDTELKSYNPTMLAQFDRAIADYRMPWKLEAI
                     LPQVRVAGEQAGKLTEKGAKLLDPTDNLQAGIPLCPPEGDAGTGMIATNCIKEKTGNI
                     SAGTSAFAMIVLEKALSKAYSELDIVTTPAGKLVAMAHANNCTSDINAWVNLFGECLS
                     VFGVNICTEELYEMLFLHALQGDADCGGLLSYGFHSGEHNVGLSEGCPVFLHPTNAHF
                     NLANFMRTHLYTAFGAMKLGMDILINQEKVEISRILGHGGIFKTEGVAQKILSSALNI
                     PLATASTASNGGAWGIALLADFLSVSDRYSLEEYLDNCIFNTTKLNLVQPDKTMSEGY
                     ERFMQRYKQGIPVVEKALFLNTD"
BASE COUNT         2131 a         1364 c         1421 g         2198 t
ORIGIN      
        1 tttttggtaa atactgatag tcatgtataa aaacctttct ttgctttcac cgacttatcc
       61 atttgacctt gaattcatcg ccggtcttac tcaatgtgag aaaggtaact atcttgattc
      121 tattgtcgat aggcaacaag gaatgcacgg ttatatgctt caattaacca cttttggtcg
      181 tggttctgtt tttgatggta agcacgtttt taccgctcat cgagggcagt tactcctctt
      241 tacacccaat gccgttcaac actaccaccg tcacccggaa agccaatatt ggcactacaa
      301 atgggtctat tttcagccga atcctaaatg gaataaatgg ctgaattggt cagatatgga
      361 agaaaaaata ggacgtatca ccattagcga tgatcgttac tttcaggaaa ttagccaact
      421 tttcagtaaa attgaattag aacttaaatc cagtaaattt tttaaagaag atattgcaac
      481 aagtttgttg gaatacttac ttatgaaatg tatttccgcc gagaaaattg aggttattcc
      541 tgtcattgat caacgcattt tgacagtttg tgatttaatc ttaacgaatt tggctaaaaa
      601 tgagagctta gaagcaatgg cagaaaaagt atttttatcg ccatctcgcc tctcacatct
      661 tttcaagcaa tcgctgggga tcagcttagt gcaatggcgg gaattacaac gcatttccga
      721 atccaaaaaa ttgctttatt tctctaacat ctctatctct aatattgcga aatccttagg
      781 ctatgaggat tctctctact tttccaaaat tttcaaaaag cataccgggc tgtcaccctc
      841 tgagtttaga accttagaac ggcgtaaaga cagtgaaatg tgatcttgat ctcactttac
      901 tttgcatagc agaattttcc atatcgataa caaaaagtta cctgtttttc tctcattcaa
      961 aagcataaga tacaaacagc tttaatagtg ctgtttctat tagagaaggg atatcaagct
     1021 ctgtgagtga tattcagtga aataacttct tatgtatgag ggaagaacaa tgaaaaaacc
     1081 aacgttcaag cacgcattat tttcaagtat tttagcttta gcctgttacg gctcggcatt
     1141 tgccaatgat gttgttaata tctccaaaat tgacggtatg ccgtggttta atcgtatggc
     1201 agagggtgtt gtgcaagcag gcaaagataa caatatcaat gcttatcaag ttggtccatc
     1261 aaataccgat gcaccacaac aagtaaagtt aattgaagat ttaattgcga aaaaagttag
     1321 tgctatttcc atcgtcccta acgatgcaaa tgccttagaa ccggtactga aaaaagcgaa
     1381 acagaacggt attgtggtat taaccaatga gtcagtcgga caacctagtg ccgattggga
     1441 tattgaaatt atcgataatg cacaatttgc agcagactat gtagaagaag tagcaaaagc
     1501 tatgggcgga aaaggcggct atgtgattta tgttggtggt ttaaccgtgc ctcaacataa
     1561 tctgtgggct gatttattcg tgaaatacca gaaagagcat tatccggata tgtttgaagt
     1621 gaccagcaga atgcctgttg ccgaaaatat taatgatgca cgccgtacca ccattgattt
     1681 agtgaaagcc cattccgatc ttaaagcggt cgtttctttt ggttcacaag gtcctattgg
     1741 tgcagggttg gcagtgaaag aaaaacgctt aaaaaataaa ttaaatgtct tcggaatgat
     1801 gattccttcc caagcagcca atttaattaa aagcggtgat attacaatgg gaattaccta
     1861 tgacccggca aatgcaggtt atgctttagc tgctgtcgct gccaaagtat taaaaggcga
     1921 aaaaattgag gatggattag agatcccaac ggtcggtaaa gccaaagtag atagtgagaa
     1981 aaaactactt caattccaca atgtgctgaa agtcaccaag gaaaatatcg ataaattata
     2041 ttaatttgct tgaaatatgg gctgatagcc agcccatatt ttccctcagg aggcacttat
     2101 ggcatttatt tcgatgcggc atatcagtaa aacattccac ggcataaaag cattaaatca
     2161 agtcaatctc tcattagatt atggcgaagc actttgtctt gccggtcaaa atggctgtgg
     2221 aaaatcaacc ttgatcaaaa ttctttccgg cgtttatcaa ccggataaag gggctgaaat
     2281 tcaaattggt gcaacccaat ataccaagct gacaccacag gaatcgattg ataaaggtat
     2341 tcaagtgatt tatcaagatt tagccctctt ccccaattta accgtggcag aaaatattgc
     2401 cattaacctg caccgaaaat ttggctgggt gagtcaagat cacattcatc aggttgccct
     2461 cactgctatt caaagcatta atgcaaatct agacctgaat gcaatattag aagatttacc
     2521 gatcgctcaa caacagttag tcgctatttg tcgagcgtta gcacaaaatg ccagattatt
     2581 gattatggac gagccgactg cctcacttac tgcaaaagag gtcaaagatc tgttagatgt
     2641 cgtcatcaaa cttaaaagca aaggcattag catcatcttt gtcagtcata aattgcaaga
     2701 agtaatgagt gtttccgata gcgtattagt gctgaaagat ggcaatatgg ttgggcaata
     2761 tcctatccgt gaaatgaatg aaaaacgcct tggtttccta atgactgggc tggaaatcga
     2821 ttacaagcgg ttagatttgc cggatttttt gcaaagaaaa accgtattgg aagtccgcaa
     2881 tttaacgctg cctcatcagt atcaagagat taacttttcg ctacgagaag gggaaattgt
     2941 tgcccttaca ggattgctcg gttcagggcg tactgagctt tgcctaagtt tattcggcat
     3001 tactcaacca caatccggcg atattttatt aaacggtgaa aaagtgatat ttaacactaa
     3061 ccgtgatgcc attgccaaag ggattgctta tgtatcggaa gatagaatgc acaccgggct
     3121 gattatggca gagtcgattc atcacaatat tatttcgact atttttcaca aaatcaccga
     3181 taaattacag attattcaat cctcgaaggc ttatgcaagt agccaagcct tgattgattc
     3241 attaaagatt aaagtatccg attcagattt gcctgttaat accctttccg gagggaacgc
     3301 acagcgagtt tcgattgcaa aatggctggc aatcgacccc aaaattatta ttttagatgc
     3361 cccgactatt ggggtcgata tcgccaataa agaagggatc ttccaaatta ttaaatcatt
     3421 ggctcagcaa ggcattgcag ttatttttgt gactgatgaa gtggaagaga cctactacaa
     3481 cagccatcgg gtaattgtaa tgaaaaaagg tcgcattgtt gatgaaatct tgccggcata
     3541 ttccagtgaa aaatcgattg cggaggttat ttatgaaaat cagcaataat catattcttt
     3601 ccagtacgtt actgatttta atcctattat taaccctatt tactcgtgat tttgcaacta
     3661 ttgagaacct ttatgatgtt gtaaataatt acgcaatgtt aatgatttta gcttgtggtt
     3721 tatttgtggt attgctgtct ggcggcattg atatttcttt ccctgctatc actattattt
     3781 cccaatatgt tatggtcact ctgatcggta aatatgatgt aggatttgcc ggcgtttttg
     3841 ttatttcttc caccatcggc atattattag gttttattaa tgctctattt gttaatcggc
     3901 ttagtgtccc ttccattatt attacgatct caacactcaa tattttttat ggcggactac
     3961 tctattttac caaaggtgta tggctttatg agtatcccga atggtttacc caagaaatcg
     4021 tgttctttaa acagataact gctgagggag tggaatttgg attatcgttt caagcattaa
     4081 tggctatttt ggcggtgata ttaaccggct taattaccaa taaactcact attggtcgaa
     4141 aaatttacgc tcttggtggt aatccggatg caacctcccg tattgggttt aatctcttat
     4201 ttctccatct atttgcatac ggttatatgg gctttatggc aggcttggca ggcatagtgc
     4261 aatcttatac cgtacaatct gtcgcaccgg attcactact gggttatgaa ttaaccgtcc
     4321 tagctgccgt tgtattaggt ggtgctagtt tatcaggtgg tagaggcacc ctgcttggta
     4381 cagtaatggg cgtaatgtta ctggcgatta ttcaaaacgg gctaaattta atgaatgtat
     4441 cgtcttactg gcaaaccatt attaccggta tcatcattat cttaagtatt agtgcaacag
     4501 ccttaagtca gcggaaaaaa caaggagcat aacgatgaaa agtaaactgg atccaacgat
     4561 tttctatctc ttagcactct tcgcagttgc agttatcgcc tttagtattt tcattccgga
     4621 tattttttgg tctgtcagta actttcaatc tatggcatca caaattccag tattaggtat
     4681 tttaacccta gcaatggcat taccgatgtt aacaggtgga ataaatcttt ccattattgc
     4741 cggaatgaat gcttgctcat taattattgc ctactttatt acgcaatata ccggtatttc
     4801 ttggttattt attgctattt tattaagcct ttgctgtgcc ttagccattg gattactcaa
     4861 cggcacatta attgccatta ttcgtgtttc accaattttg gcaaccttag gcacaatgac
     4921 tttaattaac ggcattaata ttctagtcac taacggttca accattgcga actttccgga
     4981 aacaatgttg agtattaatg cttcagcctt tttatggatt ccgctaccca ttctaatctt
     5041 tctctttctt gctgggctga tttggttttt cttagaaaaa actgcaatgg gtaaagcggt
     5101 ttatttcatc ggcaataatg aaaaagccac ctattattcg ggtattaata ccaaaaaagt
     5161 gctgatttgg gtttatgtta tctcctcaat tctttgtgta tttgcagcta ttttgatgat
     5221 gtctaaactc aattctgcaa aagcctctta tggtgattcc tatttgttga tctccatttt
     5281 agccgcagta ttgggcggta ttaaccctga tggaggtaaa gggaaactat tagggattat
     5341 gctcgcctta gttttattac aagtgattga aagtggctta aatatgctcg gcattagcag
     5401 ttacattaca atgattttat ggggaagctt attgttattc tttatcttcc tccaaaaaaa
     5461 tcgccttttc cgactaacac agtaatggtg gtagattatg caaaatgaaa aacaactcat
     5521 tgaacaaggt aatactgtta ttggtattga attgggctca acccgaatta aagcggtgtt
     5581 aattagctca gatgggacga ttttagcaac cggtggggcg gattgggaaa atcgactgat
     5641 tgacggtatt tggacctatc atcaaaatga aatatgggaa aaactacaag ctgcttatat
     5701 ggatttaagc aagacagtca aagaaaaata tcacacaacc attcgcacag ccaaagcatt
     5761 gggaattagt gcgatgatgc acggctactt acctttcgat aaacacggaa atcagctggt
     5821 tgcctttaga acttggcgaa acaacattac cctaacctca tcggaaaaat tgaccgcact
     5881 ctttcaatac ccaattccgc aacgttggag tattgctcat ttacaccaag cattgcttaa
     5941 ccaagagcct cacttgccgg aaattgatta cctgacaacc ctctcaggtt atgtgcattg
     6001 gcaactgacc gatcacaaag tgttagggat tggcgatgct tccggcatgt tcccgataga
     6061 tactgagttg aaatcctaca atccaacaat gctggctcaa tttgatcgag ccattgccga
     6121 ctaccgtatg ccttggaaat tggaagcgat tttgccacaa gttcgtgttg ctggtgaaca
     6181 agcgggcaaa ttaaccgaaa aaggtgcaaa attgttagat ccaaccgata atttgcaagc
     6241 tggcattcca ctttgtccac ctgagggtga tgccggaaca ggtatgattg caaccaactg
     6301 tatcaaagaa aaaaccggta atatttctgc cggcacctcc gcatttgcaa tgattgtgtt
     6361 agaaaaagcc ctatccaaag cctattcaga gctggatatt gtgacgaccc cggcaggtaa
     6421 attagtggca atggctcacg ctaataattg cacctctgat attaatgcgt gggtgaattt
     6481 attcggcgaa tgcttatcgg tttttggcgt aaatatctgc accgaagaac tttatgaaat
     6541 gttatttctc catgccttgc aaggcgatgc agattgtgga gggctattat cctacggctt
     6601 ccactccggt gaacataatg tcggtttatc cgaaggctgt ccggttttct tgcatccaac
     6661 gaatgcccat tttaacttag caaactttat gcgaacccat ctctataccg cttttggtgc
     6721 aatgaaatta ggaatggaca ttctcattaa ccaagaaaaa gtggaaattt cccgtattct
     6781 cggtcacggt ggtatattta aaacagaagg tgttgcacaa aaaattctgt cttccgcact
     6841 gaatattcca ttggctaccg caagtactgc ttcaaatggc ggagcttggg gaattgcatt
     6901 actggcagac tttctcagcg tatcagaccg ttacagttta gaagaatatt tagataattg
     6961 cattttcaac accaccaaac tcaatctggt tcaaccggat aaaacaatga gtgaaggtta
     7021 tgaacgcttt atgcaacgtt ataagcaagg cattcctgtg gtagaaaaag cgttgttttt
     7081 aaatacagat taaataatta taatttaagt atca
//
LOCUS       DANLZC010000127         7073 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.127, whole genome shotgun sequence.
ACCESSION   DANLZC010000127 DANLZC010000000
VERSION     DANLZC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.127"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            19..1281
                     /locus_tag="PXE44_002065"
     CDS             19..1281
                     /locus_tag="PXE44_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL5589540.1"
                     /translation="MGTLNSFLGIIVLLFIAFLFSSNKRAINVRTVLGALALQVAIGA
                     LVLYVPAGRDALNAMATGVSKVISYGNEGISFLFGGLVSDKMFEVFGGGGFVFAFRVL
                     PVIIFFSALISLLYYIGVMQWVIRILGGLLQKALGTSKAESMSAAANIFVGQTEAPLV
                     VKPYISKMTESELFAIMAGGLASIAGSVMAGYAGMGVPLTYLIAASFMAAPAGLLFAK
                     IMVPQTEKFRDELEAVDLEKPANILDAAASGASSGMQLALNVGAMLIAFVALIALVNG
                     ILGGIGGWFGYDTLNLGQIFGWVFKPLAWVIGVPWEEAEIAGQMIGLKLAVNEFVGYL
                     EFAKYLAPESTVHLTDKTVAIITFALCGFANFSSIAILIGGIGAMAPSRRGDIARLGI
                     KAVIAGSLANLMSATIAGLFIGLGGAVL"
     gene            1426..2202
                     /gene="deoC"
                     /locus_tag="PXE44_002066"
     CDS             1426..2202
                     /gene="deoC"
                     /locus_tag="PXE44_002066"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL5589541.1"
                     /translation="MNLKKSAEIALSLMDLTTLNDNDTDEKVIALCQQANTKFGTPAA
                     ICIYPRFIPIARKALKQQGIEQVKIATVTNFPHGNDDIEIAVAETKAAVAYGADEVDV
                     VFPYKALIAGNEQIGFDLVKQCKAVCAANNVLLKVIIETGELKSAELIRKASEISIQA
                     GADFIKTSTGKVAVNATLESARIMLETIRDLGVADRVGFKAAGGVKTSEEAAQYLALA
                     ESILGNDWVSASHFRFGASSLLANLLATLNDEQQQAVQGY"
     gene            2223..2942
                     /gene="deoD"
                     /locus_tag="PXE44_002067"
     CDS             2223..2942
                     /gene="deoD"
                     /locus_tag="PXE44_002067"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL5589542.1"
                     /translation="MATPHINAPEGAFADVVIMPGDPLRAKYIAETFLEDAKEVTNVR
                     NMLGYTGTYKGRRISVMGHGMGIPSCSIYAKELITEYGVKKIIRVGSCGAVRNDVKVR
                     DIVIGVGACTDSKVNRIRFRDNDFAAIADFDMTLAAHQAAKAKGIEARFGNLFSADLF
                     YSPDVEMFDVMEKYGILGVEMEAAGIYAVAAEYGAKALAICTVSDHIRTGEQTTSEER
                     QLTFNGMIEIALESVLIGDKQ"
     gene            complement(3018..3869)
                     /locus_tag="PXE44_002068"
     CDS             complement(3018..3869)
                     /locus_tag="PXE44_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL5589543.1"
                     /translation="MQTHRAIIESIFALAPKNELWATPISGLTVHAADRSIRFNGYIQ
                     DPSLCVVLLGERQICTNGHCQVFGNQEMMFCPVNLPIVTQTLSVAENQPYLAISLRLD
                     FDLIAKIITQLPPQKISENINQRTKWELENEIKDCLLRLLNLLNSPNHIHFLAPLILN
                     ELYFYLLQSEQGGYIRSLLSQDGNIAKIANATKILERKFADTLNMSALAEEVGMSVAG
                     FYAHFKSVTSMTPLQYQKSLRLNAAREKLQAGVSVSETAYQVGYESPSQFSREYKRQF
                     GESPKGR"
     gene            3984..4415
                     /locus_tag="PXE44_002069"
     CDS             3984..4415
                     /locus_tag="PXE44_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL5589544.1"
                     /translation="MKIFYHTSATATGGRDGRTQVDDGSIGFDLVGFQNESGKVGTNP
                     EQLFAMGYAACFDSAMNHVAPTLGIKPTKSSTTVAVGIGQKADGAFGLDLDITITVEG
                     ISAEEAKTLISKAHEVCPYSNATRGNVDVRLHVKVVSEFDL"
     gene            4432..5112
                     /locus_tag="PXE44_002070"
     CDS             4432..5112
                     /locus_tag="PXE44_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDL5589545.1"
                     /translation="MKMLDFETTVKTRQSIRKFLPLPMTETEIKQILADAQNAPSACN
                     TQPWLVHVASGETLARLKARFKKTFEAGINDADFEFNQTKFTGDYEPRWRNQYSHVFT
                     TSYGIPREDKAGRQVVYDDNIVGYGAPHMAFLFMPSIDGHDVNIASDVGMYSQTFLLS
                     LTARGFGGIPQLALAMFAKDVREELGIPEHYKLLHAISFGHPDWDAMQNKHHLGRVPV
                     EESATLHW"
     gene            5176..6300
                     /locus_tag="PXE44_002071"
     CDS             5176..6300
                     /locus_tag="PXE44_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL5589546.1"
                     /translation="MINLFTPYTLNNDVEIKNRLVVAPMTHSASNDDGTISEQERHFL
                     HNRAADMGLFITAATGVMRNGKAFYGQPEALDERYLASLTETANILKNQGTKAILQIF
                     HGGAQAIPALLDGANAVAPSEHANGAKALTADEVKQMIQAFANATDLAIQAGFDGVEI
                     HGANGYLIQQFFSTEFNQRTDEWGGSLEKRLRFPLAVVEAAVSVREKHQRPDFIIGYR
                     FSPEEPGEHGLTMTDTYALIDALCEKPLQYLHISLWDFGKKARRGADTNFTRMQLVHQ
                     HINGRLPLIGVGNLLTGEQIRKAYATGWAEFIALGKAVMLNPNVATLLKDNRDSEIVS
                     ELDPQQADHYGIPDRLWGYCVQGGAWLPPLKGQNGQPMDI"
     gene            6384..7001
                     /locus_tag="PXE44_002072"
     CDS             6384..7001
                     /locus_tag="PXE44_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDL5589547.1"
                     /translation="MITLHYLNSSCSHRIVWLLEALGLDYELKIYHREESGFAPMEVK
                     ALHPLGKAPVLQDGDLVLAEGNAIIQHLIARYAEGKFEPTRGSEAYSMYHYWLSISAS
                     LFSANLVTMLANRSDLGAFGDYAKAQTPLYFNHIEQTLSDKKWIVGDELTGADFSLCF
                     PLQWGRNFVEMANYPNIARYLEQVENHPAYQKANQKALKLDMNRF"
BASE COUNT         1995 a         1419 c         1607 g         2052 t
ORIGIN      
        1 tttataatta gaggttctat gggtactctt aacagctttt taggcattat tgttttacta
       61 ttcattgcat ttttattttc gagcaataaa cgtgcaataa acgtgcgtac cgtattaggc
      121 gcattagcat tgcaggtagc tattggtgca ttggtgcttt atgtgccggc agggcgtgat
      181 gccttgaatg cgatggcaac aggtgtgtca aaagtgattt cttacggtaa tgaagggatt
      241 tcatttttat tcggtggctt agtcagcgac aaaatgtttg aagtatttgg cggcggcggc
      301 tttgtgttcg ctttccgtgt acttccggtg attattttct tctctgcact catttctttg
      361 ctttactaca ttggagtgat gcaatgggta atccgtattt taggtggttt attacaaaaa
      421 gcattaggta catctaaagc ggaatctatg tctgcggcag ctaatatttt cgttggccaa
      481 actgaagctc cattggtagt aaaaccttat atcagcaaaa tgacagaatc tgaactcttt
      541 gcgattatgg cagggggctt agcttctatt gcaggttctg ttatggctgg ttacgcagga
      601 atgggcgtac cattaacgta cttaatcgca gcctccttta tggcggctcc ggcagggtta
      661 ttattcgcca aaattatggt gccacaaacc gaaaaattcc gtgatgaact agaagcggta
      721 gatttagaaa aaccggcaaa tattcttgat gcagcagctt caggtgcttc ttcaggtatg
      781 caactggcat taaacgtagg ggcaatgtta attgcattcg tggcgttaat tgcattagtg
      841 aatggtattt taggaggtat tggcggctgg ttcggttatg ataccttaaa cttaggtcaa
      901 attttcggct gggtatttaa accgcttgcg tgggtaatcg gtgtgccttg ggaagaagca
      961 gaaattgcgg ggcagatgat cggcttaaaa ttagcggtaa atgaattcgt gggttattta
     1021 gagtttgcta aatacttagc tcctgagtca accgtacatt taacggataa aactgttgcg
     1081 attattacct ttgcactttg tggctttgcg aacttcagct cgattgcgat tttaatcggt
     1141 ggtatcgggg caatggctcc aagccgtcgt ggtgatattg cccgacttgg gattaaagcc
     1201 gtcattgctg gctcacttgc caacttaatg agtgcaacca ttgccggctt gtttatcggc
     1261 ttgggtggag cagttctcta atccagtttg tataaaacca ctagaaatag tggttttatc
     1321 tttttttatc tgacgtaaac ggttgctttt ggtttgtcta tttacaagcg gtcagatttt
     1381 tataaaattt taccaaccac cacgcattta ataaaaggag acactatgaa tttaaaaaaa
     1441 tccgctgaga ttgctctttc gttgatggat ctcaccacat taaatgacaa tgatactgat
     1501 gagaaagtca tcgcattatg ccaacaagcg aacaccaagt ttggcacacc ggcagcaatt
     1561 tgcatttatc cacgttttat cccgattgcc cgcaaagcat tgaaacagca aggtatcgag
     1621 caagtaaaaa ttgcgacagt cacgaatttc cctcacggta atgatgatat tgaaattgcg
     1681 gtagctgaga ccaaagccgc agttgcttat ggggcagatg aggtagatgt ggtattccct
     1741 tataaagcat taattgcagg taatgagcaa ataggtttcg acttggtaaa acaatgcaaa
     1801 gcagtttgtg ctgccaataa tgtgttgctt aaagtgatta ttgaaaccgg cgagttaaaa
     1861 tcagcagagc ttattcgcaa agcaagcgaa atttcgattc aagcaggggc tgattttatt
     1921 aaaacctcaa ccggcaaggt ggcagtgaat gctacccttg aatctgcccg cattatgctt
     1981 gaaaccattc gtgatttagg tgtggcagac cgtgttggct ttaaagctgc aggtggggta
     2041 aaaacctcgg aagaagccgc ccaatatctt gcacttgcgg aatctatttt aggcaatgac
     2101 tgggtgagtg catcacattt tcgcttcggt gcatcaagtt tattagctaa tttattagca
     2161 acattgaatg atgaacagca gcaagctgtt caaggttatt aattaataaa gaggaacata
     2221 aaatggctac tccacacatt aacgcacctg aaggtgcttt cgctgacgtt gtaattatgc
     2281 cgggcgatcc gcttcgtgct aaatatatcg ctgaaacctt tttagaagat gcaaaagaag
     2341 tgaccaatgt tcgcaatatg ttaggctaca ccggcactta taaaggtcgc cgcatttcag
     2401 taatggggca cggtatgggg attccatctt gctcgattta cgctaaagaa ttaattactg
     2461 aatatggcgt gaagaaaatt atccgtgtcg gctcgtgcgg tgcggtacgt aatgatgtca
     2521 aagtgcgtga tattgtcatc ggtgttgggg cttgtaccga ttctaaagta aaccgtattc
     2581 gcttccgtga taacgatttt gcggcgattg ctgattttga tatgacatta gctgctcacc
     2641 aagcagctaa agcgaaaggc attgaagctc gtttcggtaa tttattctca gccgatttat
     2701 tctactcacc tgatgtcgaa atgtttgatg taatggaaaa atacggcatt ttaggcgtag
     2761 aaatggaagc agcaggcatt tatgcggtag cggcagagta tggagcaaaa gcattagcaa
     2821 tttgcaccgt ttcagaccat atccgcaccg gtgagcaaac tacctctgaa gaacgtcaat
     2881 taacctttaa tggtatgatt gaaattgcgt tagagtcagt attaattggc gataagcagt
     2941 aatttctaac ctgacaagcg gtgaaatttt gcaaaaaatt tgcagaattt gaccgcttgt
     3001 tatttttgat tcttccttta tcttcctttc gggctttcgc caaactgcct tttatattct
     3061 cgactaaact ggcttgggct ttcatagccg acttgatagg cggtttcact cacgcttact
     3121 cctgcttgta gtttttccct tgccgcattt agtcttaacg atttctgata ttgcagtggt
     3181 gtcattgaag ttacgctttt aaaatgagcg taaaaccccg ccacgctcat tcctacctct
     3241 tcagccagtg ctgacatatt tagtgtatcg gcaaattttc tttctaagat ttttgttgca
     3301 ttggcaattt tagcaatatt gccatcttga gagagtagag agcgtatata gccaccttgc
     3361 tcagattgta ataaatagaa atagagttcg tttaatatca atggtgcaag aaaatgaata
     3421 tgatttggag aattaagcaa atttagtaag cgtaacaaac agtctttaat ttcattctcc
     3481 agctcccatt tagtgcgttg attaatgttt tcacttattt tctgtggggg aagctgcgtg
     3541 ataattttgg caataagatc aaaatccaag cgtagtgaaa tcgcaagata aggctgattt
     3601 tctgcaactg ataaggtttg ggtaacaatc ggcaaattca cagggcaaaa catcatttcc
     3661 tgattaccga agacttggca atgtccattg gtacagattt gccgctcgcc taataatact
     3721 acacataaac ttgggtcttg gatataaccg ttaaatctaa tagaacgatc ggcagcgtga
     3781 acggttagcc ctgaaatggg agtagcccaa agttcatttt tcggagcgag ggcaaaaata
     3841 gattcaataa tagctcgatg agtttgcata ataatctcac ttttagaaaa ttaggcatat
     3901 tttatagaaa atcaggcaag acgacaaata gcaaaaagcg gataatatcc ccaatcaaat
     3961 aaagcattta ataggagcaa aatatgaaaa ttttttatca cacatcagca actgcaaccg
     4021 gtggtcgtga cggcagaacc caagtggatg acggttctat cggttttgat ttagtcggtt
     4081 tccaaaatga aagtggcaaa gtaggcacta atccggaaca attattcgca atgggctatg
     4141 ctgcctgctt tgacagtgca atgaatcacg ttgccccaac cttaggcatt aagccaacca
     4201 aatccagtac cactgttgca gttgggattg gtcaaaaagc tgatggtgct ttcggcttag
     4261 atttagatat taccattact gtggaaggca ttagtgcgga agaagcgaaa actttaatta
     4321 gcaaggcaca cgaagtttgc ccatattcaa atgcaacccg tggtaatgta gatgtgcgtt
     4381 tacacgttaa agtcgtgagt gaatttgact tataaaaaat aaatagggga aatgaaaatg
     4441 ttagattttg aaaccacggt aaaaacacga caatcaatcc gcaagttttt accgcttccg
     4501 atgacggaaa ctgaaatcaa acaaattctt gccgatgcac aaaatgcccc ttcggcttgt
     4561 aatacacagc cgtggttagt acacgttgcc tctggtgaaa cgctagcacg cttaaaagct
     4621 cgttttaaaa agacttttga agcgggcatt aatgatgcgg attttgagtt caatcaaacg
     4681 aaatttaccg gtgactacga accacgttgg cgtaaccaat acagccacgt ttttaccacc
     4741 agctatggca ttccccgtga agataaagcc ggtcgccaag tggtgtatga tgacaatatt
     4801 gtcggttatg gtgcgccaca tatggcattc ctatttatgc caagcattga cggacacgat
     4861 gtcaatattg ccagcgatgt tggtatgtat tcgcaaactt tcttgctctc actcaccgct
     4921 cgtggttttg gcggcattcc gcagcttgcc cttgcgatgt ttgctaaaga tgtgcgtgaa
     4981 gagttaggca ttcctgaaca ttacaagctc ctccacgcca tttctttcgg acacccagat
     5041 tgggacgcta tgcagaacaa acaccattta gggcgtgtgc cggtggaaga gagtgcgacc
     5101 ttacattggt aataaacaag cggttgattt ttcgcaaaaa tttgcgaaat caaccgtatt
     5161 ttttaggaga atacgatgat aaatttattt accccttata ctctgaataa cgatgtagaa
     5221 attaaaaacc gccttgtagt tgccccaatg acccattccg cctccaacga tgacggcaca
     5281 attagcgagc aagaacgcca tttcttgcat aatcgtgcag cagatatggg cttatttatc
     5341 accgcagcaa ccggggtaat gcgtaacggc aaagcgtttt acggtcagcc ggaagctctt
     5401 gatgaacgct atttggcaag cctgacagaa actgccaata tcttgaaaaa tcaaggcacc
     5461 aaagcgattt tgcaaatttt ccacggtggg gcacaagcta ttcctgcctt gcttgacggg
     5521 gcgaatgcag ttgcgccgtc cgagcacgcc aacggtgcta aagccctaac ggcggacgaa
     5581 gtgaagcaaa tgatccaagc tttcgctaat gccaccgatt tagctattca agccggtttt
     5641 gatggtgtgg aaattcacgg ggcgaacggc tatttaattc aacaattttt ctctactgaa
     5701 tttaatcaac gcactgatga atggggcggt agccttgaaa aacgcttgcg tttcccgtta
     5761 gcggtggttg aggcagcggt cagcgtgcgt gaaaaacacc aacgccctga ttttattatc
     5821 ggctaccgtt tctcaccgga agagccgggc gaacacggct taaccatgac cgatacctac
     5881 gccctgattg atgctttgtg cgaaaagccg ttgcaatact tgcacatttc actctgggat
     5941 ttcggcaaaa aagcccgccg tggtgcagat accaacttca cccgtatgca actggtacac
     6001 caacacatca acgggcgttt accgctgatc ggcgtgggta acttattaac cggtgaacag
     6061 atccgtaaag cttatgcaac aggctgggca gaatttatcg ccttaggtaa agcggtaatg
     6121 ctcaacccga acgtggcgac cttgttgaaa gataatcgag acagtgagat tgtcagcgaa
     6181 ctcgacccgc aacaagccga ccactacggc attcccgata gactatgggg ctactgcgtg
     6241 caaggtggtg cttggttacc gccgcttaaa gggcaaaacg ggcaaccaat ggatatttaa
     6301 tcattacaga caagcggtca tttttttgta aaaatttgca attaagtgac cgcatttttt
     6361 caattatctg aaggagaaaa attatgatta cgttacatta cctgaacagt tcctgctctc
     6421 accgcatcgt ttggttgttg gaagccttag gcttggatta cgagctaaaa atctaccatc
     6481 gtgaagagag cggttttgca cctatggagg taaaagcctt gcacccactt ggcaaagccc
     6541 ctgtgttgca agatggcgat ttggtgcttg ccgaaggcaa tgcgattatc cagcatctga
     6601 ttgcccgtta cgccgaaggg aaatttgagc cgactcgtgg tagcgaagcc tattcaatgt
     6661 atcactactg gttgtcgatt tctgcttcat tattctcggc aaaccttgtg acaatgttgg
     6721 ctaatcgctc tgatctcgga gcttttggcg actacgcaaa agcgcagact ccgctgtatt
     6781 ttaaccatat tgagcaaaca ctttcagata aaaaatggat tgtgggtgat gaactaaccg
     6841 gtgcggattt ttctctctgt ttcccactac aatgggggcg taattttgtt gaaatggcaa
     6901 attacccgaa tattgcacgc tatttagaac aggtggaaaa tcaccctgct taccaaaaag
     6961 cgaatcaaaa agccctgaag cttgatatga atagatttta atactctttt taatccctaa
     7021 cattttgtta gggatttttg aatataaaat caattaatgt gaataattat tca
//
LOCUS       DANLZC010000128         7011 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.128, whole genome shotgun sequence.
ACCESSION   DANLZC010000128 DANLZC010000000
VERSION     DANLZC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.128"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(161..508)
                     /gene="frdD"
                     /locus_tag="PXE44_002073"
     CDS             complement(161..508)
                     /gene="frdD"
                     /locus_tag="PXE44_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDL5589548.1"
                     /translation="MSLDQNPKRSNEPPVWLLFSAGGMVSALFFPVVIFIIGLLLPFG
                     LVSPDNIISFAHTWIGKLAIMVLAIFPMWAGMHRIHHGLHDLKVHTPAGNVIFYGLSI
                     LYTILVIIAVVQI"
     gene            complement(522..914)
                     /gene="frdC"
                     /locus_tag="PXE44_002074"
     CDS             complement(522..914)
                     /gene="frdC"
                     /locus_tag="PXE44_002074"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDL5589549.1"
                     /translation="MSATKRRPYVRGMKASWWKKLDFYKMYMVREATCLPTVWFCIVL
                     FYGAVALSKGTFTTDFVDFLQNPLVVILNIISLAAMLYHAATLFVMTPAVMSVMVKGK
                     HLPVSVGRNILWAATGVISVIALILVYI"
     gene            complement(924..1655)
                     /locus_tag="PXE44_002075"
     CDS             complement(924..1655)
                     /locus_tag="PXE44_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HDL5589550.1"
                     /translation="MANLDKMTIEVLRYNPESDSEPHLDRYEVPYDSQTSLLDALGFI
                     KDELEPELSYRWSCRMAICGSCGMMVNGKPKLACKTFLRDYSGFMRIEPLANFPIERD
                     LVVDLSHFIDSIEAIKPYIIDNKAPEGQRTKQTPAQLEKYRQFSMCINCGLCYAACPQ
                     FGLNPEFIGPAAITLAHRYNLDNRDNGREERMKLLSSKNGVWSCTFVGYCSEVCPKHV
                     GPASAVNQGKLESAKDYVISMIKPR"
     gene            complement(1671..3473)
                     /gene="frdA"
                     /locus_tag="PXE44_002076"
     CDS             complement(1671..3473)
                     /gene="frdA"
                     /locus_tag="PXE44_002076"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDL5589551.1"
                     /translation="MQSVNFDVAIIGAGGGGLRAAIAAAEANPNLKIALISKVYPMRS
                     HTVAAEGGSAAVIKDTDSFDNHFNDTVGGGDWLCEQDIVEYFVEHSPIEMTQLERWGC
                     PWSRREDGEVNVRRFGGMKIERTWFAADKTGFHILHTLFQTSIKYPNIVRFDEHFVLD
                     ILTDNGEARGCVAMNMMEGTLVQINANAVVIATGGGCRTYRFNTNGGIVTGDGLSMAY
                     RHGVALRDMEFVQYHPTGLPNTGILMTEGCRGEGGILVNKDGYRYLQDYGLGPETPIG
                     KPENKYMELGPRDKVSQAFWQEWRKGNTLKTAKGVDVVHLDLRHLGEKYLLERLPFIC
                     ELAKAYEGVDPAKAPIPVRPVVHYTMGGIEVDMNAETSIKGLFAVGECASSGLHGANR
                     LGSNSLAELVVFGKVAGENAARRALEVTARNQPQIDAQAKDVVARLHALANQEGNESW
                     SDIRNQMGDAMEEGCGIYRTQESMEATVNKIQELKERYKNISVKDKSSVFNTDLLYKI
                     ELGFILDVAQSIACSAVERKESRGAHQRLDYVERDDVNYLKHTQAYYNAEGTPTIKYS
                     DVKITKSQPAKRVYGAEAEAQEKVKKAAEAQAQQ"
     gene            3806..4789
                     /gene="epmA"
                     /locus_tag="PXE44_002077"
     CDS             3806..4789
                     /gene="epmA"
                     /locus_tag="PXE44_002077"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDL5589552.1"
                     /translation="MNNLQLDNIDWQPTASIPHLIQRSKIITEIRKFFTDRGLLEVET
                     PILSEFSVTDVHLSTFNTQFQSPFSPEAKTLHLMTSPEYHMKRLLAAGSGPIFQLCKV
                     FRNEEAGKRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCEPAESFSYQFVFQTY
                     VGLDPLSATKAQLVEKARKHGLQCDDDEQRDTLLQFLFSEVVEANIGQERPTAVYHFP
                     STQAALAQISSEDHRVAERFEFYYKGLELCNGFHELDDADEQIHRFEQDNLQREKMGL
                     EPQQLDVRFLAALKAGFPNCSGVALGVDRLLMLAMDAEKIEEVISFGIEKA"
     gene            4871..5761
                     /gene="rarD"
                     /locus_tag="PXE44_002078"
     CDS             4871..5761
                     /gene="rarD"
                     /locus_tag="PXE44_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5589553.1"
                     /translation="MNISGKLQGWHYALLCYLTWGTFPIYWYPINQSGMPAEQILAQR
                     VLWSALFAVFLLLIFQQGRAVINAFKQPKVLGLFVLSSFMIGVNWLVYLWAIVNEHIL
                     DASLGYFINPIFNVFVGRLILKEQLNKAQLLALLFAISGILWLAIPAGQIPWVALILA
                     ASFCIYGLIRKLAPMEPLSGLALETLLMSPFAITYLCFCYHQNILVFSELNPLQMAVL
                     LCSGIATTLPLLWFAMGARRISMSLLGMLQYISPTLQFLCGVLIFNETLSAERAVGYI
                     LVWIGVGVFLLGMKKAGVKR"
     gene            complement(5772..6383)
                     /locus_tag="PXE44_002079"
     CDS             complement(5772..6383)
                     /locus_tag="PXE44_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5589554.1"
                     /translation="MKYEAKPLIISPNHIQQRLHGKLLARALLIIALLMLAKFIYIAS
                     LSNYFTFYLMEKFQLNIAMAQLSLFTFLGAVALGAFVGGPIGDKIGRNAVIWFSFLGM
                     APFALLLPHADLFWTIVCAIFAGFIMSSAFSAMVVYAQEAVPGRVGLISGTMFGLMFG
                     IGGLSAAVLGFFADEYGLESIFKACAYLPLLGFAALGLPKRNI"
     gene            complement(6411..6946)
                     /locus_tag="PXE44_002080"
                     /pseudo
     CDS             complement(6411..6946)
                     /locus_tag="PXE44_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251153.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         2054 a         1577 c         1353 g         2027 t
ORIGIN      
        1 aaaaatttga aaaaagatct tttttgcaga tatttaatac aaaacgatcg ttttatcggc
       61 aaaatttcta atatttgcaa attttcttat aaattcgacc gcttgttagc atattaaaaa
      121 tgaaaagacc atctgcttta tacaagatgg tctttctcat ttaaatctgc acgactgcga
      181 tgattactag aattgtgtat aagatagaca atccgtagaa aatcacatta cctgctggcg
      241 tgtgaacttt caggtcgtgt aatccatgat ggatacggtg cattcctgcc cacataggga
      301 aaatcgctaa taccataata gcgagcttgc cgatccaagt atgggcaaag gaaatgatat
      361 tatctggtga tactaacccg aatggcagta ataaaccgat aataaagatt accacaggga
      421 aaaataatgc acttaccatt cccccagcac taaaaagcaa ccataccggc ggttcatttg
      481 aacgttttgg gttttgatca agactcattt tcttcccctt cttaaatata aaccaagatt
      541 aatgcaatta cgctaattac gcctgttgct gcccaaagaa tatttcttcc aaccgagact
      601 ggtaagtgtt tacctttaac catcacggac atgacggcag gtgtcatcac aaaaagtgtt
      661 gccgcatgat ataacattgc tgccaatgaa attatgttta ggataacaac taacggattt
      721 tgtaagaaat ccacaaaatc ggtagtaaat gttcctttac ttaacgcaac tgctccataa
      781 aataatacaa tgcagaacca aacagttgga agacaggtag cttcacgcac catatacatt
      841 ttatagaagt cgagtttttt ccaccagctg gctttcattc cccttacata aggtctacgt
      901 tttgttgctg acatttcttc ctcctatctc ggtttgatca ttgagataac ataatctttc
      961 gcactttcaa gtttaccttg gtttacagcc gaagcagggc cgacgtgttt cggacagact
     1021 tccgagcagt aacccacgaa agtacagctc caaacaccgt ttttgctgct gagtagcttc
     1081 atacgctctt cacgaccatt atcacggtta tccaagttat agcggtgagc caatgtaatc
     1141 gcagcaggtc cgataaattc agggttcaaa ccaaattgtg ggcaagccgc ataacataaa
     1201 ccgcagttga tacacattga gaattgacga tatttctcta attgtgccgg cgtttgttta
     1261 gtacgctgtc cttccggtgc tttattatca ataatataag gtttaattgc ctcgatgcta
     1321 tcaataaagt ggcttaaatc cactactaag tcacgctcaa tcgggaagtt cgccagcggc
     1381 tcaattcgca taaaaccgct gtaatcacgc aggaatgttt tacaagctaa cttcggctta
     1441 ccgtttacca tcattccgca agaaccacaa atcgccatac ggcaagacca gcgataagaa
     1501 agttccggtt ctaattcgtc tttaatgaag cccaatgcat ctaaaagtga agtttggctg
     1561 tcatacggca cttcatatct gtctaaatgt ggctcgctat ctgattcagg gttgtagcgt
     1621 agcacttcga tggtcatttt atctaaattt gccattttac tactccttct ctattgttgt
     1681 gcttgagctt ctgctgcttt tttgactttt tcttgggctt cagcctctgc accatacaca
     1741 cgtttagccg gttgtgattt agtgattttc acatcgctgt atttaatcgt cggtgtaccc
     1801 tctgcattgt agtacgcttg tgtgtgtttt aagtagttca catcatcacg ctcaacatag
     1861 tctaaacgtt ggtgagcccc acgagattct ttacgctcta ccgcagagca agcaatggat
     1921 tgagccacat ctaaaatgaa gcctaattca attttgtaga gtaaatcagt gttaaacaca
     1981 cttgatttat cttttacgct gatgttttta taacgctctt ttagctcttg gattttgttt
     2041 acagtcgctt ccatactttc ttgggtacgg taaataccac aaccttcttc catcgcatca
     2101 cccatttggt tacggatatc cgaccaagat tcattaccct cttggttcgc taatgcgtgc
     2161 aagcgagcca ccacatcttt tgcttgtgca tcaatttgtg gctggttgcg agcagtcact
     2221 tctaacgcac ggcgagccgc attttcaccg gcaactttgc cgaacaccac aagctctgct
     2281 aatgagttag aacccaaacg gttcgcaccg tgcaagcctg atgaagcaca ttcacctact
     2341 gcgaataagc ctttaattga ggtttccgca ttcatatcca cttcaatacc acccatcgtg
     2401 tagtgaacca ccgggcgaac aggaatcggc gctttagccg gatcgacccc ttcataggct
     2461 ttcgctaatt cacaaataaa cggtaaacgc tcaagtaaat atttttcacc taaatggcgt
     2521 aaatctaagt gaactacgtc cacgcctttt gcggttttta aggtgttccc tttacgccat
     2581 tcttgccaga atgcttgtga aaccttatca cgagggccta attccatata tttgttttcc
     2641 ggtttgccga taggtgtttc cggtcctaaa ccataatctt gcaggtaacg gtagccatct
     2701 ttattaacta aaataccacc ttcaccacga caaccttctg tcatcaaaat accggtattt
     2761 ggcaaacctg ttgggtgata ttgtacgaac tccatatcac gcaaagccac accgtgacga
     2821 tacgccattg ataaaccgtc gccggttaca ataccgccgt tagtgttaaa gcgataagta
     2881 cggcagccgc caccggttgc aatcacaact gcatttgcat tgatttgaac aagtgtgcct
     2941 tccatcatat tcattgccac acaaccacga gcttcgccgt tgtcggtgag aatatccagc
     3001 acaaaatgct catcaaaacg cacaatattc ggatatttaa tcgaagtttg gaacaaagta
     3061 tgcaaaatgt ggaaaccggt tttatccgcc gcaaaccaag tacgctcaat tttcattcct
     3121 ccaaaacggc gaacattcac ttcaccgtct tcacgacgac tccaagggca accccagcgt
     3181 tctaattgtg tcatttcaat cggcgaatgc tctacaaaat actccacaat atcttgctca
     3241 cataaccagt caccaccacc gacggtatca ttaaagtgat tgtcgaaaga gtcggtatct
     3301 ttaattaccg ccgctgaacc accttctgct gcaaccgtat ggctacgcat tgggtaaact
     3361 tttgaaatta aagcaatttt tagatttgga tttgcttcag ccgccgcaat agcagcacgc
     3421 aagccaccac caccggcacc tataatcgcg acatcaaaat taacactttg cataatgcac
     3481 tccgaaagtt aaataactta attcattaaa aagtaatcat attgtgccac tatttgagca
     3541 atgagataac ctatttttac aaatttgtaa cataaaaaat gtaattttgt gatctacctc
     3601 aaaaaaggta ataaagcttc ataaaggtat tgagaatggc tcttaattgg acagtgatag
     3661 aaagaaaatt tctaatgttt aaaatgagaa aatttaattt gttgcaaaaa agttaaaacc
     3721 ctctctttat ttttcttgat cggtaactag ggcaactcac ttttttaggc taaaatagcc
     3781 tcacattttc aagcggtcat tttttatgaa caatttacaa ctcgataata ttgattggca
     3841 accaacagcc tctattcctc acctgatcca acgttcaaaa atcattacgg aaatccgcaa
     3901 gttctttacc gatcgagggc ttttagaggt agaaaccccg attttaagcg aattttcggt
     3961 gacagacgtt catctttcga ctttcaatac gcagtttcaa tcgccttttt cccctgaggc
     4021 gaaaacactc catctgatga ccagcccgga atatcatatg aagcgactac ttgctgctgg
     4081 gagtggaccg attttccaac tgtgtaaagt atttcgcaac gaggaagccg gtaagcgaca
     4141 caaccctgaa tttacgatgc tggagtggta tcgcccgcat tttgatatgt atcgcttaat
     4201 caatgaagtg gatgatttac tgcaacaaat tttggactgc gaaccggcag aatcattcag
     4261 ctatcaattt gtgttccaaa cctatgtcgg attagatccg ctttctgcca ccaaagcaca
     4321 acttgtggaa aaagcccgaa aacacggctt gcaatgtgat gatgatgaac agcgtgacac
     4381 cttgttacaa tttttattca gcgaggttgt cgaagccaat atcgggcaag aaagaccaac
     4441 tgccgtttat catttccctt ccacccaagc ggcattagct caaattagct cggaagatca
     4501 ccgtgttgcc gaacgttttg agttttacta caaaggcttg gaactctgta acggcttcca
     4561 cgaattagac gatgctgatg agcaaattca tcgcttcgag caagataatc tgcaacgtga
     4621 aaaaatgggg ctggagccac agcaactaga tgtgcgtttt cttgccgcac tcaaagcggg
     4681 ttttccaaac tgctcaggtg tcgcccttgg ggttgatagg ctgttaatgc tggcaatgga
     4741 tgcggaaaaa attgaagagg ttatttcttt cggcattgaa aaagcctaat tttttatgcc
     4801 gttttgtgat ttgcaatttg caaaaaaatc gacaaatatg accgcttgta cactcgttta
     4861 ggaaagcaaa atgaatattt caggcaaatt acagggttgg cactatgccc tactgtgcta
     4921 cttaacttgg ggaacctttc cgatttattg gtatccgatc aaccaatcgg gaatgcctgc
     4981 cgagcaaatt ttggctcaac gggtgctttg gtctgcccta tttgctgtgt ttctgctgct
     5041 gattttccaa caagggcgag cggttattaa tgcctttaaa cagccaaagg tactcgggct
     5101 gttcgtgctt tcttctttta tgattggcgt gaactggctg gtttacttat gggcaatcgt
     5161 caatgagcat attttagatg ccagtctagg ttattttatc aaccccattt tcaacgtgtt
     5221 tgtcggtagg cttattctaa aagagcagct gaataaagcc caattattgg ctctgctgtt
     5281 tgcaattagc ggcattttat ggcttgctat tcctgccgga caaattccgt gggtcgcatt
     5341 gattctagcc gccagtttct gtatttacgg gctgattcgt aaacttgccc caatggaacc
     5401 tttgtctggt ttggcgttag aaactttgct gatgtcgcct tttgccatca cctatctctg
     5461 cttttgttac caccaaaaca ttttggtatt cagtgaactc aatcctttac aaatggcagt
     5521 gctactctgc tccggcattg ccactacctt accattgcta tggtttgcaa tgggtgcaag
     5581 acgcatttca atgtcgctac tcggtatgct gcaatatatt tcacctaccc tgcaattttt
     5641 atgcggtgtg ctgattttta atgagacact ttccgccgaa cgtgctgtag gctacattct
     5701 ggtttggatt ggggtggggg ttttcttatt gggtatgaaa aaagctggtg tcaaacgcta
     5761 aaaataaaac gttaaatatt tcgttttggt agccctaatg cagcaaaacc aagtagtggc
     5821 aaataggcac aggctttaaa aatactctcc aagccatatt catccgcaaa aaaacccaat
     5881 acggcggcac ttaagccgcc aataccaaac attaaaccga acattgtgcc ggaaatcagc
     5941 cctactctgc ctggtacggc ttcttgagca taaaccacca tagcggagaa cgctgatgac
     6001 ataataaaac cggcaaaaat cgcacacaca atcgtccaaa agagatctgc gtgaggcaac
     6061 aacaaagcaa aaggtgccat acctaagaaa gagaaccaaa tcaccgcatt tcgtccaatc
     6121 ttatcgccaa tcggcccgcc gacaaaagcg cctaaagcaa ctgcacctaa aaaggtaaat
     6181 agagagagct gtgccattgc tatattgagt tggaattttt ccattaaata aaaggtgaaa
     6241 tagttactga ggctcgcaat ataaataaat ttcgctaaca tcaataaagc gataattaat
     6301 aatgctctgg ctaataattt accgtgtaag cgttgttgaa tatggtttgg tgaaataatg
     6361 agcggtttgg cttcatattt cacccaacgg cttactttat ataaaaccca tcataatgcc
     6421 aataatacaa atgcaacaac ccaaatcacc gcactttgcc ctaacggcac aataaataat
     6481 gcaactaaga gcggacctaa cgccgatcca ctgtttccac ctacttggaa aagcgactga
     6541 gctgtaccaa atttaccgcc cgatgccatt cgagccaccc gagaggcttc agggtgaaaa
     6601 atggcagagc ctatccccat taacgcagcg gctatcagta acacgccaaa attcggcgaa
     6661 aaggcaagta aaatcacgga aagaagcgta atcagcatac tgacgggcaa taaattcggt
     6721 tttgggtatc ggtataaagc ccaatacccg gctgaataat agatgcggta atttgataaa
     6781 tgagtgaaat cataccaatt tgagcaaaac tcagttggta attttctttt agcattggat
     6841 agatcgaaac cattactgct tgaactaaat cattcaacaa atgagaaagg gaaatcagca
     6901 gtaaaatggg caaaaccatt ttttgagcgt tatgttgtgt gtgcattctt tattcgtttt
     6961 ttataaatga aaaatattct actcgcaaat aatgaatgtg caagcggtca a
//
LOCUS       DANLZC010000129         6909 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.129, whole genome shotgun sequence.
ACCESSION   DANLZC010000129 DANLZC010000000
VERSION     DANLZC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.129"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..602)
                     /locus_tag="PXE44_002081"
     CDS             complement(<1..602)
                     /locus_tag="PXE44_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5589555.1"
                     /translation="MPNFSFTHLYKKHTKELPTVTLLSALIGLSQVSYANPLPLGNTT
                     GTNNLAVGEGSFALGTGSQAIGKNAIATGGNISPQEFNVELENFRDLVNQIEELRTQI
                     AEQEKQGSVNQQLQDALKSQIAQYDTILERVREKQAQQAILANSVISMRKELVNRRNQ
                     LTELENNFNTSFLTGKNKQEAYQKFSTVIESLDWDKLSKT"
     gene            complement(796..2025)
                     /locus_tag="PXE44_002082"
     CDS             complement(796..2025)
                     /locus_tag="PXE44_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDL5589556.1"
                     /translation="MQFREINPSPRLLMCPGPTDADPRVLRAMSAPILGQFDPEFTSL
                     MNETMEMERRLFQTKNEQTYVVNSTSRGGLETVLTAAICPGDKVLIPAFGRFGYLLNE
                     ILARSGADITIIEREWGTVFEPEEIEAELKKGGYKAVAIIHGETSTSMMQPLEEIGKI
                     CKQYDAMLIVDTVATLGGVDIRVDEWGIDACIGGTQKCISAPSGTALITYNKRIEEII
                     AKRKRVEKGIRAESDVDGTLPPIPSNYLDLAQLQDYWSPRRLNHHTETTSAQYGVHEA
                     LRIILQEGLEARFARHILNDKALCAGLEAMGLKIFGDRKHKAPVVTAFHIPEGMSGPA
                     LRSDILNHFGIELATSFGPLDGKVIRIGNMGFSSQKRNVLLTLGALEAVLLSHKVKVP
                     SGDAVAAALAVYKEADK"
     gene            2298..4091
                     /gene="lepA"
                     /locus_tag="PXE44_002083"
     CDS             2298..4091
                     /gene="lepA"
                     /locus_tag="PXE44_002083"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL5589557.1"
                     /translation="MQNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLNYKAKDGEIYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAIEIDLEVVPILNKIDLPAAEPERVAEEIEDIVGIDAIDAVR
                     CSAKTGLGIEDVLEEIVKKMPAPEGDAEAPLQALIIDSWFDNYLGVVSLVRVKNGVLK
                     KGDKIKVMSTGQSYNVDRLGIFTPKQVDTTELKTGEVGWVVCAIKDILGAPVGDTLTH
                     HHNPATEVLPGFKKVKPQVYAGLFPISSDDYEAFRDALGKLSLNDASLFYEPETSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVVQTSGETIYVDSPSKLPA
                     INNIAEIREPIAECNMLVPQEFLGNVITLCVEKRGVQTNMVYHGNQIALTYEIPMGEV
                     VLDFFDRLKSTSRGYASLDYGFKRFQTADMVRVDIMINGERVDALAIIVHRANAAYRG
                     RELVEKMRELIPRQQFDIAIQAAIGNHVIARSTVKQLRKNVLAKCYGGDVSRKKKLLQ
                     KQKEGKKRMKSLGNVEVPQEAFLAILHVGKD"
     gene            4178..5137
                     /gene="lepB"
                     /locus_tag="PXE44_002084"
     CDS             4178..5137
                     /gene="lepB"
                     /locus_tag="PXE44_002084"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL5589558.1"
                     /translation="MAQFMPIIFLGIIYGIWQWLDSMQLPNTISIILVALVIICGGFW
                     AFYKFSADPRRKQAIVLQQKRLGRELTAEEIAEIQPKSAVGEFFASLFGILFFVTVLR
                     SFLFEPFQIPSGSMEPTLRVGDFLVVNKFSYGIKDPIWQNTLIETGKPERGDVIVFKA
                     PAQPHVDYIKRVVAVGGDKIKYDFATQQLTVTKANGETKVFEYSEGKPNPEFFYHGEM
                     QVERTEKGNVTHQILNHPMAFNYAPYFYQQEGQAEGEWVVPQGYYFVMGDNRDNSEDS
                     RFWGFVPERNIVGKATFIWLSLDKKANEFPTGLRFSRMFTSIN"
     gene            5183..5857
                     /gene="rnc"
                     /locus_tag="PXE44_002085"
     CDS             5183..5857
                     /gene="rnc"
                     /locus_tag="PXE44_002085"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL5589559.1"
                     /translation="MQLERLQKKLGYQFHNLDYLIQALTHRSAATKNNERLEFLGDSI
                     LNFTIGKVLYDKFPKANEGELSRMRATLVREQTLAIIARKFDLGDYLKLGPGELKSGG
                     FRRESILSDCVEAIIAAIYLDQDMAKAMEKVEDWYSDLLKDISPGDSQKDPKTRLQEF
                     LQGRKLPLPEYDVLDIKGEAHNQTFRVTCKVINVEEVFIGIGTSRRKAEQNAAEKALA
                     VVKTKK"
     gene            5960..6877
                     /gene="era"
                     /locus_tag="PXE44_002086"
     CDS             5960..6877
                     /gene="era"
                     /locus_tag="PXE44_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL5589560.1"
                     /translation="MTEQIQTPAKTYCGFIAIVGRPNVGKSTLLNKILGQKISITSRK
                     AQTTRHRIVGIHTEDQYQAIYVDTPGLHIEEKRAINRLMNRAASSAIGDVDLIIFVVE
                     GTKWTEDDEMVLNKLRSAKAPVLLAINKIDNIKEKEELLPHITELSQKFDFAEILPIS
                     AQRGKNVHILQKFVRQSLRQGEHHFPEEYVTDRSQRFMASEIIREKLMRFTGEELPYS
                     VTVEIEQFKMNERGVYEINGLILVERDGQKKMVIGAGGQKIKTIGIEARKDMERLFDN
                     QVHLELWVKVKAGWADDERALRSLGYMDE"
BASE COUNT         2126 a         1255 c         1468 g         2060 t
ORIGIN      
        1 ttagtttttg ataatttatc ccagtctaat gattctatta ctgtagaaaa tttttgatat
       61 gcttcttgtt tattttttcc tgttaaaaaa ctagtattaa aattattttc caattctgtt
      121 aactgatttc ttctattaac taattctttc cgcattgata taacactatt ggctaatatt
      181 gcttgttgtg cttgtttttc acgtacacgc tctaaaatag tgtcatattg agcaatctga
      241 ctctttaaag catcttgtaa ttgttgatta acagagcctt gtttttcttg ttcagcaatt
      301 tgagtacgta actcttcaat ttgattaact aaatcacgga agttttctaa ctccacattg
      361 aattcctggg gggagatatt accaccggtt gcaatggcat tcttgccgat tgcttgagaa
      421 cctgtgccta acgcaaaact accttcacct acagcaaggt tattggtgcc agtggtgttg
      481 cctaaaggaa gtggattggc ataagagact tggctaagcc ctatcagagc gctaagcaag
      541 gtaacagtag ggagctcttt tgtgtgtttt ttatacagat gggtgaaact aaagtttggc
      601 atatcaaaat ttcctcatta aaacttttac ttttcattct taaaaaaatg ctccgtgtca
      661 taatatttaa tttgattaaa tgaaatagga aaagattaaa tttgtgaaaa tattttgagt
      721 ttctgtggtt ttactgtgga taaatatcca cagtattaat tattagtgag ttgtattgta
      781 aatttaggaa ggtggttatt tatcagcctc tttatagact gccaaggcag ctgcaaccgc
      841 atcgcctgaa ggcactttta ctttatggct taaaagtacg gcttctaacg cacctaaggt
      901 taaaagtacg ttacgttttt ggcttgagaa acccatatta ccgatacgga ttacttttcc
      961 atctaagggg ccgaatgagg tggctaattc aataccgaag tggtttaaaa tatcactacg
     1021 caatgctggg ccactcatac cttctggaat gtggaatgcc gttacaacgg gggctttatg
     1081 tttgcgatcg ccaaaaattt tcaaacccat tgcttctaaa cctgcacata atgctttgtc
     1141 gtttaaaata tggcgagcaa aacgagcttc taagccttct tgtaaaataa tgcgtaacgc
     1201 ttcgtgtaca ccatattgtg cagaagtggt ttctgtgtgg tgatttaaac ggcgtgggct
     1261 ccagtaatct tgtagctgtg ccaagtctaa gtagttactt ggaattggcg gtaatgtgcc
     1321 atctacatca gactctgcac gaatgccttt ttccacacgt ttacgttttg caataatctc
     1381 ttcaatccgt ttgttataag tgattaaagc tgtgcctgat ggggcggaaa tacatttttg
     1441 tgtaccgccg atacaagcat caatgcccca ttcatccaca cgaatatcta caccgcctaa
     1501 ggttgcaacg gtatctacaa ttaacattgc atcatattgt ttacagattt tgccgatctc
     1561 ttctaacggt tgcatcatag aggttgaggt ttcgccgtga ataatagcaa ctgctttata
     1621 gccgcctttt ttcaactctg cttcaatttc ttctggttca aatacggttc cccactctct
     1681 ttcgataatg gtaatatctg caccgctacg agctaaaatt tcatttaata aataaccaaa
     1741 acgaccaaat gcaggaatta acactttatc gcctgggcaa atcgctgcgg ttagaacagt
     1801 ttctaagcca ccacgagagg tggagttgac tacataagtt tgctcatttt tggtttggaa
     1861 taaacggcgt tccatttcca ttgtttcatt cattaatgag gtaaactcag ggtcaaattg
     1921 ccctaaaatg ggtgcagaca ttgcacgcaa aacacgtggg tcagcatctg ttgggccagg
     1981 gcacattaat aaacgagggg agggattaat ttctctgaat tgcataatta tttcctataa
     2041 ttgaatgaat taaaaaaact tatggattct ataataaaaa tttgtgattg ctcaatgttt
     2101 tttatatttt agagaaatta tttgcaatta gggtgagtgc aagcggtcat ttttctcaag
     2161 aaatttgcac atttttagga aaaaataacc gcttattgct atttgttttc tttgcaaaac
     2221 cttgtatttc aagtacaatt ccccgattaa tttttgataa acaaataaca aaacaggaca
     2281 ttaactgccc gatgaaaatg caaaatattc gtaacttttc gattatcgct catatcgatc
     2341 acggcaaatc gactttatca gaccgcttaa ttcaaacttg tggtggctta tcagatcgtg
     2401 aaatggaggc tcaagtatta gattcaatgg atcttgagcg tgagcgtgga attactatta
     2461 aagcacaaag tgtaacctta aactataaag cgaaagatgg cgaaatttat cagctcaact
     2521 ttattgatac gccagggcac gtggatttct catatgaagt ttctcgctcg cttgctgctt
     2581 gtgaaggtgc tttattagtg gttgatgcag ggcagggggt ggaagcacag acgttggcga
     2641 attgttatac cgcaattgag atagatttag aggttgtgcc aattttaaat aaaatcgatt
     2701 taccagcagc cgagcctgag cgtgtggcag aagagattga agatattgtc ggcattgatg
     2761 ccattgatgc agtacgctgt tcagctaaaa caggtttagg cattgaagat gttttagaag
     2821 aaatcgtgaa gaaaatgcct gcaccagaag gcgatgcgga agcaccatta caagcgttaa
     2881 ttatcgactc ttggttcgat aactatttag gcgttgtgtc tttagtgcgt gtgaaaaatg
     2941 gcgtgctgaa aaaaggcgat aaaattaaag tgatgagtac agggcagtct tacaatgtag
     3001 atcgccttgg tattttcacg ccaaaacaag tggatactac cgagctaaaa acaggtgaag
     3061 taggttgggt agtttgtgcg attaaagata ttttaggggc accagtgggc gatacattaa
     3121 cccaccacca caacccagca actgaagtat taccgggctt taagaaagta aaaccacagg
     3181 tttatgcagg gttatttccg attagttcag acgactacga agccttccgt gatgctttag
     3241 gcaaattaag cctaaatgat gcgtcattgt tctatgagcc agaaacttca agtgcgttgg
     3301 gctttggctt ccggtgtggc ttcttaggct tgttgcatat ggagatcatt caagagcgtt
     3361 tagagcgtga atacgatctt gatttgatta ctaccgctcc gaccgtagtt tacgaagtgg
     3421 tgcaaaccag tggcgaaacg atctatgtgg atagcccatc gaaactccca gcaatcaaca
     3481 atattgcaga aattcgtgag ccgattgcag aatgtaatat gcttgtgcca caagaattcc
     3541 taggcaacgt gattaccctt tgtgtggaaa aacgtggcgt acagaccaat atggtctatc
     3601 acggtaacca aatcgcctta acttacgaaa tcccaatggg cgaagtagtg ttagacttct
     3661 tcgaccgctt aaaatcaacc tctcgtggtt atgcttcact cgattacggt tttaaacgtt
     3721 tccaaacggc tgatatggtg cgtgtggata ttatgattaa cggcgagcgt gttgatgcgt
     3781 tggcgattat cgttcaccgt gcaaatgcgg cgtatcgtgg acgtgaatta gtggaaaaaa
     3841 tgcgtgaact tattccacgc caacaatttg acatcgctat tcaagcggca attggtaacc
     3901 acgtcattgc tcgctcaacg gtaaaacagt tgcgtaaaaa cgtattagca aaatgttacg
     3961 gtggtgatgt aagtcgtaag aagaaactgc tacaaaaaca aaaagagggt aaaaaacgaa
     4021 tgaaatctct cggtaacgta gaagtgccac aagaagcatt tttggcaatt cttcatgtgg
     4081 gtaaggatta attgtcaata ttcgtgtaaa atacgaccgc ttgcaagcgg tcatattttg
     4141 agaaaacttt gcaattttga ataaaaagga gcattcagtg gcacaattta tgccaattat
     4201 ttttctaggt attatttatg gtatttggca atggcttgat tcaatgcaac tgccaaatac
     4261 tatttccatt attttagttg ccttggttat tatttgtggt ggattttggg cgttttacaa
     4321 attcagtgct gacccaagac gtaaacaggc gattgttcta caacaaaaac gtttaggcag
     4381 agaattaacc gctgaagaaa tagcggaaat tcaacctaaa tccgcagtag gcgaattttt
     4441 tgcctcactt ttcggcattt tattctttgt tacagtactt cgctcgttct tatttgaacc
     4501 gtttcaaatt ccaagtggtt caatggagcc aaccttgcgt gtaggcgatt ttttagtcgt
     4561 aaataaattc agctacggca ttaaagatcc tatctggcaa aacaccttga ttgaaacggg
     4621 caaaccagaa cgaggcgatg tgattgtatt caaagcaccg gcacaaccac acgttgatta
     4681 tattaagcgt gtggtggcag taggtggtga taaaattaaa tatgattttg caactcagca
     4741 attaacggtt actaaagcca atggcgaaac aaaggtattt gaatacagcg aaggcaagcc
     4801 aaatcctgag tttttctatc acggtgaaat gcaggtagaa cgcactgaaa aaggtaatgt
     4861 aactcatcaa attttaaatc acccaatggc atttaactat gcaccttatt tctaccaaca
     4921 agaagggcaa gcagaaggtg aatgggttgt gccacaagga tactattttg tgatgggtga
     4981 taaccgtgat aacagtgaag acagtcgttt ctggggcttt gtgcctgaga gaaacatcgt
     5041 tggtaaagcc acttttattt ggttaagttt agataaaaaa gcaaatgaat tcccaacggg
     5101 gcttcgtttt tctagaatgt ttacctcaat caattaatct taacaggggc gtatgagata
     5161 cgccccttta ttcacataaa ctatgcaact agaacgatta cagaaaaaat taggttatca
     5221 atttcataac cttgattacc tcattcaggc cttaacacac cgcagtgcgg caaccaaaaa
     5281 caatgagcgt cttgaatttc taggcgattc aattttaaat tttactattg gtaaagtatt
     5341 atacgacaaa tttccaaaag cgaatgaagg tgagcttagc cgtatgagag caactcttgt
     5401 gcgtgagcaa actctagcga ttattgctcg caaatttgat ttaggcgact acctaaaatt
     5461 aggtccagga gagctaaaaa gtggtggttt tcgccgagaa tcgattttat cggactgtgt
     5521 tgaagcgatt attgcggcca tttatctcga tcaagatatg gcaaaagcaa tggaaaaggt
     5581 ggaagattgg tattcagatt tgcttaaaga tatcagtcca ggcgattcac aaaaagatcc
     5641 aaaaacccgc ctgcaagagt ttttacaagg tagaaaattg ccattgcctg aatatgatgt
     5701 attagatatt aaaggcgaag ctcataacca aactttccgt gtaacctgta aagtgattaa
     5761 cgtcgaggaa gtgtttatcg gcattggcac cagccgccga aaagccgaac aaaatgccgc
     5821 agaaaaagcg ttagcggtgg ttaaaacgaa aaagtaagat agcgcaagcg tcctcgcttg
     5881 tgctaaagat ttaattaaat ggtacaagcg tggacgcttg caccatcaaa ttcattccac
     5941 agaatgaaag gaaaacaaaa tgacagaaca aattcaaact cctgcaaaaa cctattgtgg
     6001 ttttattgcg attgttggtc gcccaaatgt agggaaatca acattattaa ataagatttt
     6061 aggacaaaaa atttccatta cctcacgcaa agctcaaacg actcgccatc gtattgttgg
     6121 tattcacact gaagatcaat accaagcgat ttatgtagat accccaggct tacacataga
     6181 agaaaaacgt gcgattaatc gtttaatgaa ccgtgcagca agttctgcta ttggcgatgt
     6241 agatctcatc atctttgttg tggaaggcac aaaatggaca gaagatgatg aaatggtgtt
     6301 aaacaaattg cgttcggcaa aagcccctgt tttattggca atcaataaaa ttgataacat
     6361 caaggaaaaa gaagaacttc tgccgcatat taccgagctt tcacaaaaat ttgattttgc
     6421 tgaaattctg ccaatttcgg cacaacgggg taaaaatgtg catattttac agaaatttgt
     6481 ccgtcaatca ctgcgtcaag gtgagcacca ctttcctgaa gagtatgtga cagaccgctc
     6541 tcaacgcttt atggcatcag aaattattcg tgaaaaacta atgcgtttta caggtgaaga
     6601 gttaccttat tcggtaacgg tagaaatcga acaatttaaa atgaatgagc gtggggtgta
     6661 tgaaatcaat ggcttgattt tagtagaacg agatggtcag aaaaaaatgg ttattggtgc
     6721 gggggggcaa aaaatcaaaa ccatcggcat tgaagctcga aaagatatgg aacgcctatt
     6781 tgacaaccaa gtgcaccttg agctttgggt gaaagtaaaa gctggttggg cggatgatga
     6841 aagagcattg agaagtttag ggtatatgga cgagtagttg gctcccgtta tagcattaat
     6901 atcgtaatc
//
LOCUS       DANLZC010000130         6794 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.130, whole genome shotgun sequence.
ACCESSION   DANLZC010000130 DANLZC010000000
VERSION     DANLZC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.130"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            62..493
                     /locus_tag="PXE44_002087"
     CDS             62..493
                     /locus_tag="PXE44_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5589561.1"
                     /translation="MSNQIALLLMSHGSFARAALASAELIVGQQENVAAIGVVAVDNV
                     ADIKRQMEQTIAELNTDKGLIILTDIVGGTPMNLASSQLTHPNVFACSGLNLPLLLEV
                     LMSRDGNLQEIRENLPLAYQNGFSLRTQQDFVSQEDDDDLL"
     gene            480..947
                     /locus_tag="PXE44_002088"
     CDS             480..947
                     /locus_tag="PXE44_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL5589562.1"
                     /translation="MICFSRIDDRLIHGQVVTTWVNLHRIEQIIVLNDAVAADPTQKQ
                     ILTMAAPQGIKVVAFGIEKFGEVLQKTQITRRTMLLFTNSIDVLRTVELGVSIDKLNV
                     GGMRYQARREQLSKAVSVTPEERQAFKTLLEKGVEVTVQMVPNDEKLNLQEII"
     gene            950..1717
                     /locus_tag="PXE44_002089"
     CDS             950..1717
                     /locus_tag="PXE44_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL5589563.1"
                     /translation="MLTALLVACWAGICALDDIGTQMIRRPLLIAPVVGLIMGDLETA
                     LIIGATLEVMWMGIGNVGAYSAPDMISGTAIGTALGIAAGEASTAVALAVPTSLLAQQ
                     LLILYRSGVCALNPIAERWAASGDFDKIFRIHYIPMLIAFSIRAIPTFLAVYYGADII
                     AQAVSALPAKVITGLNVAGKIIPAIGIGLLMLMMIKKAELWTFLIAGFTLAVYLKLSV
                     LPITLIALPLALIYDLAVSKKAEAAPTKTADEEDYDL"
     gene            1714..2520
                     /locus_tag="PXE44_002090"
     CDS             1714..2520
                     /locus_tag="PXE44_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL5589564.1"
                     /translation="MSNKITKQDLNKVFWRMQVLNVTNNFQSMQAIGFLSSFIPVLKR
                     LYKDRPQEERAEAMKRHLKFFNSHVNSDALILGIAAAVEETTKEDEKETVTAIKTGLM
                     GPLAGLGDSLLKFTWLPICGSIGAALALSGNVLGPILMFLLYNSVNIATKYYGIHLGY
                     SKGVDLIHGAGNNILQRLGNMANVVGLMVIGALIASVVKVKVVSQIAAGDNVIKFQEM
                     LDKVMPGLLSLLLTVVIYWILKKTNGKYAATMILATMGICIGLVYAGILG"
     gene            2520..3557
                     /locus_tag="PXE44_002091"
     CDS             2520..3557
                     /locus_tag="PXE44_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDL5589565.1"
                     /translation="MDSVYDIIRLEQGRYLFRQQPAQSVQIFFLDNPDSKDSKWLAET
                     DLAEFELKLSACEARPFFLIQSANGEQIVAERTLPVAGMNNFRDMGGYVAHQGKRVKW
                     GKLYRSDHLHNLRDEGVAYLDKLGIQTVIDYRSPNEVAKYPNPPINGREQTFRLDPNA
                     HTAELAAQFSADKHDEDRNLVNKIIAQKAEGNLINRYDIVMAQYRNFVEKAECQTAFA
                     EMLRLATDPENAPLVQHCRGGKDRTGFGAMLLLGILGVSKADIIADYMLTHYNRLARN
                     EEKMAIYRTFTQDQDVLDYLLSLIDTQPEFIEQSLNTIETQYGTIEQYAQRVLGITAK
                     EIEALRANYLA"
     gene            3647..4867
                     /gene="agp"
                     /locus_tag="PXE44_002092"
                     /pseudo
     CDS             3647..4867
                     /gene="agp"
                     /locus_tag="PXE44_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628146.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
     gene            5175..>6794
                     /locus_tag="PXE44_002093"
     CDS             5175..>6794
                     /locus_tag="PXE44_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL5589566.1"
                     /translation="MSQDIQADLKKILALLSRNRRFQSLFLLLLRSEQFINSDALSTE
                     LQLSVRTVKKEIKLLKEKLEPYGIQIFSRPFYGYKLLIQFEELHTQLKQYFQISQPMT
                     VNNKFESRVNAILRRLLVSQRALKVEDFQSELYLSFTSALTKEFSQVKALLGKYGLIL
                     EAKPYHGMNIVGESYRKIMLTVRLYKYFTQLEHPFGIKEFEQCFACPMTEKIRERLAK
                     TIIHFNLVFSDIYAERFIIYLRYFYNQQPELLLPKLNFNYQQTNEYQFVIKLLEQLRE
                     QDSRFEFNPEVIRFLTYIAIASTDLYRFKDCNSDNYPLLLDKAWEIHRFIFTQFSAYL
                     NLSEIHDDTCRKDLLKMLIPIAMKMMLSISDDVDLGLFNQTELHRNPILAHTVEKLVD
                     DIEKNYGYSLSIREQYFIFDIFSGMVNRIELERKPLRLAIIALNGRLSTQQLKRNIKR
                     YFSDYILKIETKVLYELAIMEDKPYDYYLCNEYGKNMAIPYKPIYFADSSLNENEYAT
                     SLNEVFYHAYQYDEVLPSIKSIEIEAQYRLNE"
BASE COUNT         2108 a         1231 c         1435 g         2020 t
ORIGIN      
        1 cccttgttta tctgtataac attttattta atagaatgac ttataaatca aggagtgact
       61 tatgtctaat caaattgcgt tattgttgat gagccacggc agttttgcga gggcggcgtt
      121 ggcgagtgcc gagctgattg tcgggcagca ggaaaatgtg gcagctatcg gcgtagtagc
      181 agtggataat gtagcggaca ttaaacggca gatggagcaa accattgctg agttaaacac
      241 cgacaaaggc ttgattattc tcaccgatat tgttggtggt acgccgatga atcttgcctc
      301 ttctcaatta acccacccta atgtttttgc ctgttcgggt ttgaatttac ctttattgct
      361 agaagtgctg atgagccgtg acggcaattt gcaagaaatt cgggaaaatc taccgcttgc
      421 ataccaaaac ggatttagcc tacgcacaca acaagatttt gtttcacagg aggacgatga
      481 tgatctgctt tagccgtatt gatgaccgtt taattcacgg tcaagtagtc accacttggg
      541 ttaatttaca ccgtattgaa caaattattg tgttaaatga tgcggtggca gcggatccaa
      601 cccaaaaaca gattttaacg atggcagcac cgcagggaat taaagtggtc gcgtttggca
      661 ttgaaaaatt tggtgaagtg ttgcagaaga cacaaattac ccgccgtact atgttgttgt
      721 ttaccaatag cattgatgtg ttacgcactg tagaactagg tgtatcgatt gacaaattga
      781 atgtcggcgg aatgcgttat caagctaggc gtgagcagtt aagtaaagcg gtttcagtta
      841 cgccagaaga gcgtcaagca tttaaaacct tattggaaaa gggtgttgaa gtaacggtac
      901 agatggtacc gaatgatgaa aaacttaact tacaggagat tatctagcta tgctgactgc
      961 actacttgtt gcctgctggg caggtatttg tgccttagat gatattggca cacagatgat
     1021 ccgccgtccg ttgttaatcg caccggtggt cggattgatt atgggggatt tggaaacggc
     1081 gttgattatt ggtgctacgc ttgaagtgat gtggatgggg atcggcaatg ttggtgcata
     1141 ttctgcaccg gatatgattt ccggaaccgc tattggcacc gcacttggta ttgctgctgg
     1201 tgaagcttct actgcggttg cactcgcagt accgacctca ctcttggcac agcagttact
     1261 cattctttac cgctccggcg tatgtgcatt gaacccaatt gctgaacgct gggcggcaag
     1321 cggtgatttt gacaagattt tccgcattca ctatatccca atgttaattg ccttttctat
     1381 tcgtgctatt ccaacgttct tagccgtcta ttacggggcg gacattattg cccaagcggt
     1441 ctctgctctg ccggcaaaag tgattaccgg attaaatgtt gcaggcaaaa ttattcctgc
     1501 catcggtatc ggtttgctga tgttaatgat gattaagaaa gccgaacttt ggacgttctt
     1561 aattgctggc tttacgttgg cggtttatct caaattatca gtattgccga ttaccttaat
     1621 tgcactaccg cttgcgttga tttacgactt agcagtaagc aaaaaagctg aagctgcacc
     1681 aaccaaaacc gcagatgaag aggattatga cctatgagca ataaaattac caaacaagat
     1741 ttaaacaaag tgttttggcg gatgcaggtg ctgaacgtca caaacaactt ccaatcgatg
     1801 caggcgattg gatttttaag tagctttatt ccggtattaa aacgacttta taaagatcgt
     1861 ccacaggaag aacgagcgga agcaatgaaa cgccacttaa aattttttaa cagccacgtt
     1921 aattctgatg cattgatttt aggaattgcc gctgcggtag aagagactac caaagaggat
     1981 gaaaaagaga cagtaaccgc gattaaaacc ggtttaatgg ggccgttagc agggctggga
     2041 gacagcttgt tgaaattcac ctggttaccg atttgcggta gtatcggggc ggcattggca
     2101 ctaagtggca atgtgttagg cccgatttta atgttcttgt tgtataacag tgtaaacatt
     2161 gcgacgaagt attacggtat ccatctaggt tacagcaaag gggttgattt aattcacggt
     2221 gcgggtaata acattttgca acgactcggc aatatggcaa atgtggttgg tttaatggtg
     2281 attggcgcat taattgcctc cgttgtgaaa gtcaaagtag tttcacaaat tgccgctggt
     2341 gataatgtga ttaaattcca agagatgtta gataaagtga tgccgggact actcagctta
     2401 cttttaactg tcgtgatcta ctggattttg aagaaaacta acggcaaata tgctgcaacg
     2461 atgatccttg ctacgatggg gatctgtatc ggcttagtct atgcaggaat actcggctaa
     2521 tggacagtgt gtatgacatt attcggttgg aacagggcag atacctgttc cgccaacagc
     2581 cggctcaatc ggtacagatt ttctttctgg acaaccctga cagtaaggat agcaaatggc
     2641 tggcggagac tgatttggca gagtttgagc tgaaattgag cgcttgtgaa gcccgtccgt
     2701 tcttcctcat tcaatcagca aacggagaac agattgttgc tgaacgcacc ttacctgttg
     2761 ccggaatgaa taattttcgg gatatggggg gctatgtcgc tcatcagggc aagcgagtga
     2821 aatggggcaa gttataccgc tccgatcatc tgcataattt aagagacgaa ggcgtggctt
     2881 atcttgataa attgggaatt cagactgtta ttgactatcg cagcccgaat gaagtcgcca
     2941 aatacccaaa tcccccaatt aatggcagag agcaaacttt tcggcttgat ccgaatgcac
     3001 atactgccga gcttgctgcc caatttagtg cagacaagca cgatgaagat cgtaatttgg
     3061 tcaataaaat cattgctcaa aaagctgaag gtaatcttat caatcgctac gacattgtaa
     3121 tggcacaata tcgtaatttt gtcgaaaaag cagaatgcca aaccgccttt gctgaaatgt
     3181 taaggttggc gacagaccct gaaaatgcac cattggttca acactgccgt ggagggaaag
     3241 atcgcactgg tttcggtgca atgttactgc ttggtatttt aggcgtgagt aaagctgata
     3301 ttattgctga ttatatgctt actcattata accgccttgc tcgtaacgaa gagaaaatgg
     3361 cgatttaccg taccttcact caagatcaag atgtgttgga ctacctattg tctttaatcg
     3421 atactcaacc cgaatttatc gaacaatcgc ttaatactat tgaaacccaa tatggtacta
     3481 ttgagcaata tgcccaaagg gtattaggta ttactgcgaa ggaaattgaa gcattacggg
     3541 caaattattt ggcgtagaac ggtagggtgt aatattgaac acactacaaa tggagaaatt
     3601 tcatatgcgt tattttgcaa aacattggta taaaactacc gcttgtctga caacaatttt
     3661 cacagcattt cctctttttg ctcaaccaat tgaaaatata ccaacttccc actacgaatt
     3721 agaaaaaatc attatattta gccgacacgg gttacgttcc cctcttacag aaaaaggcag
     3781 tgatttagaa aaatcaaccc cttataaatg ggcaaattgg agtgtaccga ccggtcattt
     3841 aacccataaa gggacgctgt tggaaaccta ttttgggcag tatcttggtc aatggctcac
     3901 acataatggg ttattggaca aggcaagctg tgaaagtggg aagcagattg aggtttatgc
     3961 taatagcctg caacgtacaa ttggcactgc acaagctttg gttgccggtg ggttttcagg
     4021 ttgtaatgta caagttaaac accaccagca gattggaaag atggatccgg tgtttaatcc
     4081 aattattcgc tccgatgacc agacatttgt gcaaacagca ttaagcaaaa ttgacctcaa
     4141 taaaacccat cagtatttag cgccatccta tcatttatta agcgaaataa tcgattatgc
     4201 tcattcagaa aaatgtttgc aggaaaaaca gtgtgaattt tttaatgatg ttggtaaatt
     4261 aaggattaaa aaggataaat aaccaggtgt ttccggtacg atgcgattaa gtaaaaatat
     4321 tgtcgatgcc ttattgctgc aatattatga aggttttcct ttaaaagaga ttgcaaataa
     4381 ccagcttgat agtgatcaga aatggcaaac actcaataaa attaaaaacg attaccaatt
     4441 tatgctacga ggagatccat ttattgctaa acatatttca ttaccgctat taaaatatat
     4501 tcagcaagat ttaaatagtg aaaataaaat aacactttta gtcggacacg attccaatat
     4561 tatagcctta ctttctgcat taaatgttaa gccttataaa ttagcacatc aatatgaaca
     4621 aactccaata ggagggaaag tctttattga aatctggaaa gaaaaaagta gccaagcaca
     4681 aaaagttaaa ctggaatatg tatatcaatc tactgaccaa attcgtaaag cgattccact
     4741 aagccttgat aaaccaccgc aacatcacat tttgagtttg gattattgcc aagctgacca
     4801 atatggatta tgcgattata tggaatttaa acagttactg gaaaaagtca ttgctgatac
     4861 attgtgagat tttgacaaag tgagtgaaaa aggtagcata aaaaagtcgg gacagttgcc
     4921 agctgctccc tgtaagatgt tgtttacagc actagtaatg ctactatccc caaaaagctg
     4981 ctgaaaggca gcttttttgt tgtatataaa agtacacctt tctacgcttc attctttttc
     5041 tccaattaaa cctaatttag gacttctagt gataaattcg gtatcatatc ttaattcact
     5101 acatatcgtg caagccttaa aattgaaagg gctgacgaaa tcctatttaa ttctgacaca
     5161 cataagggaa tattatgtcg caagatattc aagccgattt aaaaaagata ttggcactat
     5221 taagccgtaa ccgtcgattt caatcattat ttttattact gttaagaagt gaacaattta
     5281 ttaatagtga tgcactttct acggagttac agctttcagt acgaacggta aaaaaagaga
     5341 ttaagttatt aaaagaaaaa cttgaacctt acggcattca gattttttct cgaccgtttt
     5401 atggctataa attattgatt cagtttgaag aattacacac gcaactaaaa cagtatttcc
     5461 aaatatcaca gccgatgacg gtaaataata aatttgagtc tcgggtaaat gcgattttac
     5521 gccgattact ggtgagccag cgtgcattga aagttgaaga ttttcagagt gagctttatt
     5581 taagttttac cagtgcctta acaaaagaat ttagtcaagt taaagccttg ttaggaaaat
     5641 atggattgat actcgaagcg aaaccctatc acggaatgaa cattgtaggt gaaagttatc
     5701 gtaaaataat gctcacagta cgattatata aatattttac ccagcttgaa catccatttg
     5761 gaattaaaga atttgaacag tgttttgctt gtcctatgac tgaaaaaatc cgtgaaaggt
     5821 tagctaagac aattattcat ttcaatttag tattttctga tatttatgct gagcggttta
     5881 ttatttactt acgctatttt tataatcaac agcctgagtt attgttaccg aagttaaatt
     5941 ttaattatca acagacgaat gaatatcagt ttgtaattaa gttactggaa cagcttcgtg
     6001 aacaagacag tcgttttgag tttaatccgg aagttattcg atttctgacc tatattgcga
     6061 tagccagtac agatctctac cgctttaaag attgtaattc ggataattac ccattgttgc
     6121 tagataaggc ttgggaaatt catcgtttta tttttactca gttttctgca tatttaaatt
     6181 tatcggaaat tcacgatgac acttgccgta aagatttgct taaaatgctg atccctatcg
     6241 caatgaaaat gatgcttagc atttcagatg atgtggattt agggctattt aaccaaacag
     6301 aactacatag aaatccaatt ttggctcata cagtagaaaa attagtggat gacattgaaa
     6361 aaaactacgg gtatagccta tctattcgag aacagtattt tatttttgat attttttccg
     6421 gaatggttaa tcgaattgaa ttagaacgta aaccgctccg actggcgatt attgccctta
     6481 atggcagatt aagcacccaa cagttaaaac ggaatattaa gcggtacttt tccgactata
     6541 ttttgaaaat tgaaaccaaa gtgctatatg agcttgccat aatggaagat aaaccttatg
     6601 actactatct ctgtaatgaa tatggaaaaa atatggctat tccctataag cctatttatt
     6661 ttgctgacag tagtttaaat gaaaatgaat atgcaacatc actcaacgaa gtattttatc
     6721 acgcttatca atatgatgag gtgttgccta gcattaaatc gattgaaatt gaagcacaat
     6781 atcgcctaaa tgag
//
LOCUS       DANLZC010000131         6467 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.131, whole genome shotgun sequence.
ACCESSION   DANLZC010000131 DANLZC010000000
VERSION     DANLZC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.131"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..707)
                     /locus_tag="PXE44_002094"
     CDS             complement(<1..707)
                     /locus_tag="PXE44_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5589567.1"
                     /translation="MSIYRRKANGPWWVDITTPNGKRIKRSTRTLVKREAQQYHDKLK
                     QEMWAEDKLEEKRKYIFEDALLHYVRAAEELKDKATKKRHAEYWLSKFAGRELSSLTA
                     QEIILNIPKKNANTKKPLSHSTQNKYVKSLSRVLNIAHKLGMLDAVPHIPKKKEPPIR
                     VRWITKEQAKQLIDKLSSDWMKSICKFALMTGARRTEILTMTWDKIDLVRKVAIVTND
                     VAKSGKARSLLLNQEAI"
     gene            complement(611..934)
                     /locus_tag="PXE44_002095"
     CDS             complement(611..934)
                     /locus_tag="PXE44_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5589568.1"
                     /translation="MEHLTINQVAKELNCHPNTVRQHLADWGFFKMVGSEVWRINRED
                     LERAKKRAGIDPIRKRSDNDRGLSLSVEENEKCRSTDEKQTVRGGLISQPQTARELRE
                     ALARL"
     gene            complement(1015..1182)
                     /locus_tag="PXE44_002096"
     CDS             complement(1015..1182)
                     /locus_tag="PXE44_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL5589569.1"
                     /translation="MQFTYGSICSGIEATSIAWHGLGKPLWFSEIEPFPCAVLAHRFP
                     NVPNGNGMAVP"
     gene            complement(1190..1564)
                     /locus_tag="PXE44_002097"
     CDS             complement(1190..1564)
                     /locus_tag="PXE44_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589570.1"
                     /translation="MHIEDKIAWWLANGETGVSSKTMAFYLGYGIRPKIEGYPHDVSD
                     FRRCFLLLETVPFLRNRIEKMAELGKVWAALAKEWHTLEALYNEEECQIRCPKTYAKL
                     REILEANEENVVRIGNLSISIG"
     gene            1714..1941
                     /locus_tag="PXE44_002098"
                     /pseudo
     CDS             1714..1941
                     /locus_tag="PXE44_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            1966..2361
                     /locus_tag="PXE44_002099"
     CDS             1966..2361
                     /locus_tag="PXE44_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5589571.1"
                     /translation="MKIHTQIRKEILSLLEAKLTDIEHFYNGQPNFIDIDEQQLAISV
                     YLDEINRQELTLCDEQWTAQLNITIYLKSVDEAEDELDEWAEKIREVVETYSAFEHLE
                     GISLSQYQYEQDQNQRTWHSATLIFDVEY"
     gene            2393..3034
                     /locus_tag="PXE44_002100"
     CDS             2393..3034
                     /locus_tag="PXE44_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589572.1"
                     /translation="MAKTTKVQGTKFRIGIGREAQKAITAITLATATLTIATSGYKKG
                     DAIEITGCGQLDGIYPVLSVTGDQVKLCEEVNWTGKDLPANYTKAKAALVQYSDQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPSTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            3117..3518
                     /locus_tag="PXE44_002101"
     CDS             3117..3518
                     /locus_tag="PXE44_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589573.1"
                     /translation="MTLREKLLANKPKLQSIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGALLFNPLNIDDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            3563..3793
                     /locus_tag="PXE44_002102"
     CDS             3563..3793
                     /locus_tag="PXE44_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL5589574.1"
                     /translation="MPERHFAEYQLFYQEQPFGLWREDYRAAQLAHLTAMINRDPKGK
                     APELSEFMPFFNRADEVEANEDDGVADYLAKR"
     gene            3861..4127
                     /locus_tag="PXE44_002103"
     CDS             3861..4127
                     /locus_tag="PXE44_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL5589575.1"
                     /translation="MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAK
                     TKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEAFHKAMLAE"
     gene            4131..4400
                     /locus_tag="PXE44_002104"
     CDS             4131..4400
                     /locus_tag="PXE44_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL5589576.1"
                     /translation="MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKY
                     RDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVRLGSHAELFG"
     gene            4475..4738
                     /locus_tag="PXE44_002105"
     CDS             4475..4738
                     /locus_tag="PXE44_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589577.1"
                     /translation="MKNVLLFIVHFFLIAMLSIIPILIILGAIERDPYYTAWIFLSVF
                     LGIGALLYFATIVSKLINKGKTQTLEEKRYAEYWNNVKVNIPK"
     gene            4798..5508
                     /locus_tag="PXE44_002106"
     CDS             4798..5508
                     /locus_tag="PXE44_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL5589578.1"
                     /translation="MSGLGKLTVTLELENAKFQSAMTKSDYEAQKFAKNFIQNMDRAR
                     NHAKEFADRSTQYLKNIEQAAKNLNKNSEFSFLSTIGGHYQSMSSGILSAAKAYTDIQ
                     NKMKLVSGSSAEAAKRLTDVFDIATKTSQSTEAVSGVYQTFAQNAQSLGLAQKDVAEL
                     TKTVSQAVAASGASSSAASNALTQFGQSLLMGKMKAQEFNSLITQTPTIIQAMAKGLG
                     MTMAEFKAAVYRAKSLLG"
     gene            5486..5776
                     /locus_tag="PXE44_002107"
                     /pseudo
     CDS             5486..5776
                     /locus_tag="PXE44_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            5766..>6467
                     /locus_tag="PXE44_002108"
     CDS             5766..>6467
                     /locus_tag="PXE44_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL5589579.1"
                     /translation="MKFSKYQLLVNKICFICGKKDCPQMKKSKDYKDFLDALEKGDVD
                     KADKIYNTKFSQFSKSFGHEMEKNLEKNRIPPIYQGVPFDSFDLYQHYSYGNGEALNL
                     SSVGLSNKIRPLVNKDKAFGKSNGSIESRFISQYKNEGRTSFSNAYDFTKEASGIADP
                     LWALGTATISGELKDIQIFPGGIARGNIHYSIRDRFTDPFDTFNFTKGEWNPNGTPYI
                     IQDSWIKSVRFSIDKK"
BASE COUNT         2055 a         1276 c         1250 g         1886 t
ORIGIN      
        1 ttaattgcct cttgattcag taatagcgat cgtgccttac ctgatttagc gacatcatta
       61 gtcacgattg ctacctttct tacaagatct atcttatccc aagtcattgt caaaatttct
      121 gtccgtctgg ctccagtcat caaagcaaac ttacagatag atttcatcca gtctgaactt
      181 aatttatcta ttagctgttt tgcttgttct ttggtgatcc agcgtacacg aattggcggc
      241 tctttctttt tcggtatgtg tgggactgca tccaacatac caagtttatg agcgatattt
      301 aatactctcg ataaagattt cacatactta ttttgcgtac tatgagatag aggctttttc
      361 gtatttgcat tctttttcgg gatatttagg ataatctcct gagctgttaa agagctgagt
      421 tcacgaccgg caaatttgga taaccagtat tcagcatgtc gcttttttgt ggctttatct
      481 tttaattctt cggccgcacg aacgtaatgg agcaaagcat cttcaaagat atattttctc
      541 ttttcctcga gtttatcctc tgcccacatt tcttgcttga gtttatcgtg atactgctga
      601 gcttcacgtt ttacaagcgt gcgagtgctt ctcttaattc tcttgccgtt tggggttgtg
      661 atatcaaccc accacggacc gtttgctttt cgtctgtaga tcgacatttt tcattctcct
      721 cgaccgacag agataaccct cggtcattat cagatcgttt tcttattggg tcaataccgg
      781 ctctcttttt tgctctttcc aaatcttctc ggttgatccg ccaaacttcc gagcctacca
      841 ttttaaaaaa tccccaatcc gccaaatgtt ggcggactgt atttggatgg caatttaatt
      901 cttttgccac ttgattaata gtaagatgtt ccattcatta ctttctccaa aataaatccc
      961 tctaaacgag ggattgtact aaataatcgt taagtctttg ccttatccac tgcatcaagg
     1021 cacagccata ccgtttccat ttggcacatt ggggaaacgg tgagctaata ccgcacaggg
     1081 gaatggctca atttcagaga accacaaagg cttacctaaa ccgtgccacg ctatacttgt
     1141 agcttcgata ccgctacaaa ttgagccgta ggtgaattgc attttttacc tatcctatcg
     1201 aaatactcaa attcccaata cggacaacat tttcttcgtt ggcttctagg atttctctca
     1261 acttagcata ggttttgggg catcttattt gacattcctc ttcgttgtag agagcctcta
     1321 atgtgtgcca ttcttttgca agtgcagccc aaactttccc aagttcagcc attttttcaa
     1381 tcctatttcg caaaaaaggg actgtctcca acaacagaaa gcaacgcctg aaatccgata
     1441 catcgtgtgg gtagccttct atttttggtc ttattccgta accaaggtaa aaagccattg
     1501 ttttactgct tacccctgtc tctccattgg caagccacca agcgattttg tcttctatgt
     1561 gcatttttcc tccaaaaatt aaccgcttgc ggaacaagtt ctacaagcgg ttgtcaagtg
     1621 ttcatttgtt caatgaacag ttcattgtga ataacaaaac cgcttgaatt acaagcggtc
     1681 tattttgttg cgtaaattta ctgttagcgt tgagccataa ccgactacga ccgctggccc
     1741 gaagatattg acggcgaggg ggacggcttc tcgcttgcct ccaaacggac gaccactttt
     1801 atgtcagccg gaatgacttt agtggagagt tcgcctggta aagatattgt cgatctcaaa
     1861 catatcccga aaagtagcca cgaagcccca ccgactaccg gtattttaag cctctacaat
     1921 cgaggcgacc gcagacgctt tagcgaaaca gttgataggt aagctatgaa aatccatacc
     1981 caaatccgaa aagaaatcct ttcattatta gaggcaaaat taacggacat cgagcatttt
     2041 tataacgggc aaccgaattt cattgatatt gacgaacagc aacttgccat ctctgtttat
     2101 cttgatgaga ttaaccgtca ggagcttacc ttatgcgatg aacagtggac agctcaactt
     2161 aacatcacga tttatctaaa atcagttgat gaggcagaag atgaattaga tgagtgggcc
     2221 gaaaaaatcc gtgaagtggt tgaaacatat tctgcgtttg aacatttaga gggcatcagc
     2281 ctttctcaat atcaatacga acaagatcaa aatcaaagaa cttggcactc agccacattg
     2341 atttttgatg ttgaatacta acaagcaaat aatccttata ggagaatcta caatggcaaa
     2401 aacaacaaaa gtccaaggca caaaatttag aattggcatt ggacgtgaag cccaaaaggc
     2461 aattacggct atcactttag ctacagcaac gcttactatt gccacatcag gctataaaaa
     2521 aggcgatgca atcgaaatta ccggttgtgg tcagttagac ggcatttatc ctgtattatc
     2581 tgtaacaggc gatcaagtca aattgtgcga agaagtaaac tggacgggta aagatttacc
     2641 ggctaattac accaaagcca aagcagcttt agtacaatac tcagaccaat tctgtgcggt
     2701 aaaaaacatt gaaaaatctg atgatacgtt aagtaccgaa gatgttacaa cggtctgctc
     2761 ggaaggtaca gaaaccgaac ccggagaaat tgaatttggc tcaattaaac tgagcttttt
     2821 tcacaagcca agtacggaaa tgcaaactcg cttacgcaaa ttgttttatg acaaatccac
     2881 ctttgcgtac aaattagaat tacctgataa ccacggcaca acctatggcg aaggtttcat
     2941 tgaggcgggt aacggcttta gtggcgaagt taaaggcaaa tatgaaggct cggtatcaat
     3001 taaaccaagc aaacgtgatt atttgctagt gtaattacaa aatattgctc caaatcgacc
     3061 gcttgtaata ttgcaagcgg tcttttttat cctaaatttt acaaaaggaa tcgacaatga
     3121 cactccgtga aaaactcctc gccaacaaac caaaattaca atctatcgaa atcaacggtg
     3181 agacctacta cctgcgtgaa gccactgttg gcgatatgaa taagcagatt tttgaaaccc
     3241 gaagctggct cattcaacaa gctgaacaag aaaatgttga attaccggca gaagatgatg
     3301 aaacctttga cgaagctctc aaccgttttg gcgaaaaata ccgccttgct caatcggttg
     3361 cctaccgttt atgtgacgaa aacggtgcat tactgtttaa cccacttaac attgacgatc
     3421 tcaatgcgat tgccgaatta gacagtaaag tgattatcga ctttaaccaa gccgtgtccg
     3481 ccccaaaaga ctcagcgagc gaagaaagtt ccaaataacc ctttcgctcg cactgggtaa
     3541 aacccttgaa gaaatcgaac aaatgcctga acgccacttt gccgaatatc agctttttta
     3601 tcaagagcaa ccttttgggc tttggcgtga ggactatcgt gctgctcaac ttgcccactt
     3661 aaccgcaatg atcaaccgtg acccaaaagg caaagcccct gaactctcgg aatttatgcc
     3721 gttttttaat cgggctgatg aagtagaagc aaatgaagat gatggcgttg cggattattt
     3781 ggcgaaaagg taaatctctt gtatttcatt gtaattctgt ctataattgg tgtaatacat
     3841 agaaatacag gagagataaa atggcgacta tcaatgatgc tttcagtttt agaacaaata
     3901 ccgaaataaa aaataccgca tttgatgtaa ttaaaaacta tggaatgaca ccctctcagg
     3961 tgtttaatat gtttttaacc gagattgcaa aaacgaaaac tattccgtta agtttgaatt
     4021 atcaacccaa tcttgaaaca aaattggcaa tgcaagaagc aaaatcaggt aaaaatgaag
     4081 tttatgcctc acttgaagca tttcataaag caatgttagc ggagtaaata atgctacaaa
     4141 tttcgccgac aaacgcatat aaaagagact ttaaaaagat tgcagccgaa ttagtcggta
     4201 gttcggaata tgtggaagta atgtattgcc taataaacca attaccattg gcggaaaaat
     4261 atagagatca cccactacaa ggtgaatggc aaggctttag agattgccat attaagcctg
     4321 atttagtgtt gatttacgct gttgaagata atctgctccg ccttgttcgt ttaggatcgc
     4381 acgctgaatt atttggataa tttctgtaaa aactaaccgc ttgcgatttt gtgctgtggg
     4441 cggtatagtt ggaatattaa caaaggaggg aaaaatgaaa aatgtacttc tatttatcgt
     4501 acactttttc ttaatcgcaa tgctctcaat tatcccaata ttaattattc ttggagcaat
     4561 cgaaagagat ccttactata ctgcgtggat tttccttagc gtatttttag gcattggagc
     4621 attgttatat tttgcaacca ttgtttctaa gttaattaac aagggaaaaa cacaaacatt
     4681 agaagaaaaa cgatatgcag aatattggaa taatgtaaaa gtcaatattc ccaaatagtc
     4741 taactgataa atgaccaaaa gctcgcttta tgcgggcttt tttattggag aaaaacaatg
     4801 agtggcttag gaaaattaac cgttacctta gagcttgaaa atgcaaaatt tcagtccgca
     4861 atgacgaaat ctgattatga ggcacaaaaa tttgccaaga actttatcca aaatatggat
     4921 agagctagaa atcacgccaa agaatttgcc gatcgctcaa cccaatattt aaaaaacatt
     4981 gagcaagcgg caaagaacct caataaaaat tctgaatttt catttttatc gactattggt
     5041 ggacattatc aatcaatgtc tagtggcata ttgagtgcgg caaaagccta tactgatatt
     5101 caaaataaaa tgaaattagt aagcggtagt tcagcagagg cagctaaacg attaactgat
     5161 gtgtttgata ttgcgactaa aacaagccaa agtacagagg ctgtttcagg tgtctatcaa
     5221 acatttgctc aaaatgccca atctctcgga ctggctcaaa aagatgtagc agaattaact
     5281 aaaacggtct ctcaagccgt tgctgcaagc ggcgcaagca gttctgcagc aagtaatgcc
     5341 ctaacccaat ttggtcaatc acttttaatg ggcaaaatga aagcccaaga atttaactcg
     5401 ctcattactc aaaccccaac cattattcaa gcaatggcaa aaggtttggg aatgacaatg
     5461 gcagaattta aagcagcggt ataccgggca aaatctttac ttggataaca ttcaagggca
     5521 aggttttgag agagtggata caccccaaat cggcgatgtg attttaatac aggtcggttc
     5581 tgatgtgccg aatcacgcag cgatttatat tggtgaacaa atggtaatcc accatagccc
     5641 gaaccgatta tctaagcgtg atttatatga cggttattgg ttacgccata cccacagtat
     5701 ttggcggcat aaactggcgg atagattgga ttttgacggc atatttaatg atataggaga
     5761 acaatatgaa atttagtaaa tatcaattat tagttaataa gatctgcttt atttgtggta
     5821 aaaaagattg tccacaaatg aaaaagagta aagactacaa agactttttg gatgctttag
     5881 aaaaagggga tgttgataaa gcagataaaa tatacaatac gaagttttca cagttttcta
     5941 aatcttttgg tcatgaaatg gaaaagaatt tagagaaaaa tcgtatacct cctatttatc
     6001 agggagttcc atttgattct tttgacttat atcagcatta ttcttatggt aatggagagg
     6061 cattaaatct ttcatccgtt ggattaagta ataaaattcg tcctctagtg aataaagata
     6121 aagcatttgg taaatccaat ggaagtatag aaagccgatt tatttcgcaa tataaaaatg
     6181 aaggacgcac atctttctcc aatgcttatg attttacaaa agaagcatcg ggtattgcag
     6241 atccgttatg ggcgttagga acagctacca ttagtggaga attgaaagat atacaaattt
     6301 ttcccggtgg tattgctagg ggaaatatcc attatagtat cagggacaga ttcactgacc
     6361 catttgatac attcaatttt actaaaggag aatggaaccc aaatggaaca ccttatatta
     6421 ttcaagatag ctggataaaa tcggtacgat tttcgataga caagaag
//
LOCUS       DANLZC010000132         6422 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.132, whole genome shotgun sequence.
ACCESSION   DANLZC010000132 DANLZC010000000
VERSION     DANLZC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.132"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            27..2651
                     /gene="aceE"
                     /locus_tag="PXE44_002109"
     CDS             27..2651
                     /gene="aceE"
                     /locus_tag="PXE44_002109"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005550031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDL5589580.1"
                     /translation="METQDWLASLDSLIREEGLERAQFIIEQVISQARAGGVALPTGV
                     TTDYVNTIPVSEQPAYPGDHKIERRIRSAVRWNAIAAVLRSQKKDLDLGGHISTFQSA
                     ATMYEVCFNHFFKAPTEKDGGDLVFFQGHAAPGMYGRAFLEGRITAEQMDNFRQEAFT
                     DGLSSYPHPKLMPEFWQFSTVSMGLGPVNAIYQARFLKYLENRGLKDTSAQKVYAFLG
                     DGEMDEIESKGALSFAGREKLNNLIFTISCNLQRLDGPVNGNGKIVQELEALFTGCGW
                     EVIKVLWGSGWDKLLEKDTSGKLKQLMMEVLDGDYLTFKSKNGAYVREHFFGRYPETA
                     ALVADMTDDEIWALRRGAHDSEKLYAAYAKAQKSDKPVVILAHQVKGYRIPEAESKNT
                     AHQSKKMSLDSLKGFRDYFELPLTDEQVESLEYVRFPEGSEEYNYLHGQRKALNGYVP
                     VRKPKFTVDFKVPELSEFQALLDEQPRGISTTMAFSRVLNTLLKDKNIGQQIVPIIAD
                     EARTFGMEGLFRQIGIYNPHGQNYVPSDRDLVAYYREAKDGQVLQEGINELGATASWV
                     AAATSYSVSNLPMIPFFIYYSMFGFQRVGDMMWLAGDQLARGFMIGGTSGRTTLNGEG
                     LQHEDGHSHIQAGVIPNCVTYDPAFAFEVAVIVQDGIRRMYGENQEDIFYYITTLNEI
                     TEQPAMPAGAEEGIRKGLYKFETVEGKGKGHVQLLSSGAIMRHVRAAAQILANDYGIT
                     ADVFSAPSFNELGRDGADVARWNLLHPTETPRVPYVAQVLQDLPTVASTDYMKMYAEQ
                     IRAYVPSKHYHVLGTDGFGRSDSRANLREHFEVDERYVVVAALAQLAKEGTVENKVVA
                     EAITKFGLNVDRINPLYA"
     gene            2855..4765
                     /gene="aceF"
                     /locus_tag="PXE44_002110"
     CDS             2855..4765
                     /gene="aceF"
                     /locus_tag="PXE44_002110"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL5589581.1"
                     /translation="MSKQINVPDIGSDEVSVTEVMVKVGDTISVDQSIINVEGDKASM
                     EVPAPEAGVVKEVLVKVGDKVSTGSPMLILESADAAPAQEAPKAEAAPAAPVATTSAV
                     VEVNVPDIGGDEVNVTEIMVKVGDSVEVDQSIINVEGDKASMEVPAPVAGVVKEILIN
                     VGDKVSTGKLIMKFETASAATAQSTPVAEAAPAQASASAIKDVNVPDIGGDEVNVTEI
                     MVKVGDSVSEEQSLITVEGDKASMEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAG
                     AAPAPAAAQAASAPAPAPQAAAPAKADAAPAGQNQSGLSQEQVVASAGYAHATPVIRR
                     LAREFGVNLDKVKGTGRKGRIVKEDIQAYVKTAVQVFEKQGGTAAAATGAANGAGLGL
                     LPWPKVDFSKFGEVEEVELSRINKISGANLHRNWVMIPHVTHFDRTDITELENFRKEQ
                     NKEAEKRKLDVKITPVVFIMKAVASALEAFPRFNSSISEDAQRLTLKKYINIGVAVDT
                     PNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIGTTH
                     FTPIVNAPEVAILGVSKSEMQPIWNGKEFEPRLMLPLSLSFDHRVIDGADGARFLSYI
                     NSVLADIRRLVM"
     gene            4965..6389
                     /gene="lpdA"
                     /locus_tag="PXE44_002111"
     CDS             4965..6389
                     /gene="lpdA"
                     /locus_tag="PXE44_002111"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL5589582.1"
                     /translation="MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGI
                     CLNVGCIPSKALLHVAKVIEEAKNAVHNGVTFGEPTIDLDQVRAGKEAVVSKLTGGLA
                     GMAKARKVTVVEGLAAFTGPNTLVARDRDGNPTTITFDNAIIAAGSRPIQLPFIPHED
                     PRVWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKD
                     VVAIYTKQIEKKFKLMLETKVTAVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPN
                     GKLIDAGKAGVEVDERGFIRVDKQMRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGMKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASEC
                     SEGMTKLIFDKDTHRVLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNAKAKKR"
BASE COUNT         1952 a         1221 c         1501 g         1748 t
ORIGIN      
        1 agaacttaag aaatgacgta gatccaatcg aaactcagga ttggttagca tcactcgatt
       61 cattaattcg tgaagaaggt ttagagcgcg ctcaatttat cattgaacaa gttattagcc
      121 aagctcgtgc aggtggcgtg gcattaccaa caggcgtaac aacagattat gtcaatacta
      181 tccctgtttc cgagcagcca gcttatccgg gtgatcataa aatcgaacgt cgtattcgtt
      241 ctgcggtacg ttggaatgcg attgcggcag tacttcgtag ccagaagaaa gatcttgact
      301 taggtggtca tatttcaact ttccaatctg cggcaactat gtatgaagtg tgttttaacc
      361 actttttcaa agcaccaacc gaaaaagatg gtggtgattt agtgttcttc caaggccacg
      421 cagcaccggg tatgtatggt cgtgcgttct tagaaggtcg tattaccgca gaacaaatgg
      481 ataacttccg tcaagaagca tttacagacg gtttatcttc ttacccacac cctaaattaa
      541 tgcctgaatt ttggcaattc tctaccgtat ctatgggttt aggaccggta aatgctattt
      601 accaagctcg tttcttaaaa tatttagaaa accgtggtct aaaagatact tcggctcaaa
      661 aagtatatgc attcttaggc gatggcgaaa tggatgaaat cgaatctaaa ggtgcattat
      721 cattcgccgg tcgtgaaaaa ttaaataact taatcttcac tattagctgc aacttacaac
      781 gtttagacgg tccggttaac ggtaacggca aaatcgtgca agaattagaa gcactcttta
      841 ccggttgtgg ctgggaagtg attaaagtgt tatggggtag tggttgggat aaactgcttg
      901 aaaaagatac ttcaggcaaa cttaaacagt taatgatgga agtgcttgat ggtgactatt
      961 taaccttcaa atccaaaaat ggtgcttacg ttcgtgaaca cttcttcggt cgttaccctg
     1021 aaactgcagc attagttgct gatatgacag acgatgaaat ttgggcatta cgccgtggtg
     1081 cacacgattc tgaaaaactt tatgcagcct atgcaaaagc acaaaaatcg gacaaaccgg
     1141 ttgtaatttt agctcaccaa gtgaaaggct accgtattcc tgaagcggaa agtaaaaata
     1201 ctgctcacca atcgaaaaaa atgtcattag acagcttaaa aggcttccgt gactacttcg
     1261 agctaccatt aaccgatgag caagtagaaa gcctagagta tgtaagattc cctgaaggtt
     1321 ctgaagagta caactactta cacggacaac gtaaagcctt aaacggttat gtgcctgtac
     1381 gtaaaccgaa atttacggtt gatttcaaag tgcctgaatt aagcgaattc caagcattat
     1441 tggatgagca accacgtggt atttcaacca caatggcgtt ctctcgtgta ttgaatactt
     1501 tattgaaaga taaaaatatc ggtcagcaaa tcgtacctat tattgcggac gaagcccgta
     1561 ctttcggtat ggaaggttta ttccgtcaaa tcggtattta taacccacat ggtcaaaact
     1621 atgtaccgtc agaccgtgat ttagtagctt actaccgtga agcaaaagat ggccaagtgt
     1681 tacaagaagg tatcaatgag ttaggtgcaa cagcctcttg ggtggcagca gcgacttctt
     1741 attctgttag caacttaccg atgatcccat tcttcatcta ctactcaatg tttggtttcc
     1801 aacgtgtagg cgatatgatg tggttagccg gtgaccaatt agctcgtggc tttatgattg
     1861 gtggtacttc aggtcgtaca acattaaacg gtgaaggttt acaacacgaa gatggtcata
     1921 gccatattca agcaggtgta atcccgaact gcgtcactta tgaccctgca tttgcctttg
     1981 aggttgcggt tattgttcaa gacggtattc gtcgtatgta tggtgaaaac caagaagata
     2041 tcttctacta cattactacc ttaaatgaaa ttaccgaaca gccggcaatg ccggcaggtg
     2101 cggaagaagg tatccgtaaa ggtttatata aatttgaaac cgttgagggt aaaggtaaag
     2161 gtcacgtaca attattaagc tcaggtgcaa ttatgcgtca cgtgcgtgca gctgcacaaa
     2221 ttctcgcgaa tgactacggc attacagcag atgtattctc agcaccatca ttcaatgaat
     2281 taggtcgtga tggagcagat gtggctcgtt ggaacttatt acacccaact gaaacaccac
     2341 gtgtaccgta cgttgctcaa gtgttacaag atttaccgac tgttgcttca accgactata
     2401 tgaaaatgta cgcagagcaa atccgtgctt atgtaccaag taaacactat cacgtgttag
     2461 gtacagacgg tttcggtcgt tcagacagcc gtgcaaactt acgtgaacac ttcgaagttg
     2521 atgagcgtta tgtagtagtt gcagccctcg ctcaattagc gaaagaaggt acagttgaaa
     2581 acaaagtggt tgcggaagct attactaaat tcggcttaaa tgtagatcgt attaacccat
     2641 tatatgcgta atttctaata ctctctccct tgatgggaga gaggcagcta aaggtttcgt
     2701 ttagaaactt ttagcagaga gggtattagt tttatttgca aaaatgttga gaaaatcaac
     2761 cgcttgcatt cccttctccc tgatttttct tcttacaaag aaaaatctgt ccctcccaca
     2821 atgggagagg gggtaactca agaaggattt caaaatgtca aaacaaatta atgtgccgga
     2881 catcggttcg gatgaagtca gtgtgactga agtgatggta aaagttggtg atactatttc
     2941 agtagatcaa tcaattatta acgtagaagg cgataaagcc tctatggaag tgccggctcc
     3001 tgaagcaggt gtggtaaaag aagttttagt gaaagtgggt gataaagttt caaccggttc
     3061 accaatgtta attttagagt cagcagatgc tgctcctgct caagaagcac caaaagcaga
     3121 agcagctcct gctgctccag tagcaacaac ttcagccgtt gttgaagtaa atgtacctga
     3181 tatcggtggc gatgaagtta acgtaaccga aattatggtt aaagtaggtg atagcgttga
     3241 agtggatcaa tctatcatca acgttgaagg tgataaagcc tcaatggaag tgcctgcacc
     3301 ggttgccggc gtagtaaaag aaatcttaat caatgtgggt gacaaagttt caactggtaa
     3361 attgattatg aaatttgaaa cggcttccgc agctacagca caatctacac cggttgcgga
     3421 agctgctcca gctcaagctt cggcatcagc gattaaagat gtaaatgtac cggacatcgg
     3481 tggtgatgaa gtaaacgtaa ctgaaattat ggttaaagtg ggtgattctg tttcagaaga
     3541 acaatcttta atcacggttg aaggtgataa agcttcaatg gaagttccgg ctccatttgc
     3601 cggtgtggtg aaagaaattt tagtgaaatc aggtgataaa gtttcaaccg gttcattaat
     3661 tatgaaattt gaagtagcgg gtgcggcacc ggctccggcg gctgctcaag cggcttcagc
     3721 tcctgctcct gctccacaag cagcagctcc ggcgaaagct gacgcagctc cggcaggtca
     3781 aaaccaatca ggtttaagcc aagaacaagt ggtggcttca gctggctatg cacacgcaac
     3841 accggtaatt cgccgtttag ctcgtgagtt tggtgtaaac ctagacaaag taaaaggtac
     3901 aggtcgtaaa ggccgtatcg ttaaagaaga tattcaagca tacgttaaaa cggctgttca
     3961 agtatttgaa aaacaaggcg gaacagcagc ggcagcaaca ggtgcggcaa acggtgcagg
     4021 cttaggttta ttaccgtggc cgaaagtaga tttcagcaag tttggtgaag ttgaagaagt
     4081 tgaattaagc cgtatcaata agatttccgg tgcgaactta caccgtaact gggtaatgat
     4141 tcctcacgtt acacatttcg atcgcacaga cattaccgag ttagaaaact tccgtaaaga
     4201 gcaaaataaa gaagcggaaa aacgtaaatt agacgtgaaa attacccctg tggtcttcat
     4261 tatgaaagca gtggcaagtg cattagaagc cttccctcgt ttcaacagct caatttctga
     4321 agatgcacaa cgtttaacct tgaaaaaata catcaatatc ggggttgcgg ttgatacgcc
     4381 aaacggttta gtggtgccgg tgttcaaaaa tgtgaacaaa aaaggcatta tcgaactttc
     4441 tcgcgaatta atggaagtct cgaaaaaagc ccgtgaaggt aaattgacag catctgatat
     4501 gcaaggtggc tgtttcacaa tttcaagttt aggcggtatt ggtacaacgc actttacgcc
     4561 aatcgtgaat gcaccggaag tggctatttt aggtgtgtct aaatctgaaa tgcaaccgat
     4621 ttggaatggc aaagagtttg aaccacgctt aatgcttccg ctttcattat cattcgacca
     4681 ccgtgtgatc gatggtgctg atggtgcgag attcttaagc tatattaata gcgtgttggc
     4741 agatattcgt cgtttagtga tgtaattcgg taaagctcct cctaaaaaag tccctcttta
     4801 gaaaagaggg gtagggggag atttgataga cgtaatttac gaagagaata aattatttgg
     4861 tagaaatttg tgtttgattg attgctctct cacttcgctt tatattctgc caaatctccc
     4921 ttacccctct ttgctaaagg gggggaataa aaagaggaaa aactatgagc aaagaaatta
     4981 aaacccaagt tgtcgtactt ggtgcaggtc cagcaggtta ctcagcggca ttccgttgtg
     5041 ccgacttagg cttagaaaca gtaattgttg agcgttattc aaccttaggt ggtatttgct
     5101 taaacgtagg ttgtatccct tctaaagcat tattacacgt tgcaaaagta attgaagaag
     5161 ctaaaaatgc agtacacaac ggtgtgacct ttggtgagcc aacgattgat ttagatcagg
     5221 tacgtgctgg taaagaagcc gttgtttcaa aattaaccgg tggcttagca ggaatggcta
     5281 aggctcgtaa agtgacagta gtagaaggct tggctgcatt tactggtccg aatacgttag
     5341 tcgctcgtga tcgtgacggc aatcctacaa ctatcacgtt cgataatgcg attatcgcag
     5401 caggttctcg tccgattcaa ctaccattta ttccacacga agacccacga gtttgggatt
     5461 caaccgatgc tcttaaatta aaagaagtac cgaaaaaact ccttattatg ggtggtggta
     5521 ttatcggttt agaaatggga acggtatata acgcgcttgg ttcagaagtg gaagtggttg
     5581 aaatgttcga ccaagtaatt cctgctgcag ataaagatgt tgtagcgatt tacaccaaac
     5641 aaatcgagaa aaaattcaag ctaatgcttg aaaccaaagt aaccgcagta gaagctaaag
     5701 atgacggtat ctatgtatca atggaaggta aggcttgcaa cgatactaaa cgttatgatg
     5761 cggtattagt cgctatcgga cgtgtgccaa acggtaaatt aattgatgcc ggtaaagctg
     5821 gagtggaagt agatgagcgt ggctttattc gtgtagataa acaaatgcgt accaatgtgc
     5881 cacacatctt tgctattggt gatattgtcg gccagccgat gttagctcac aaaggtgtac
     5941 acgagggaca cgtggcagca gaagtgattg caggaatgaa acattacttc gatccgaaag
     6001 tcattccgtc catcgcttat actgaaccag aagtggcttg ggttggtaaa actgagaaag
     6061 agtgtaaaca agaaggcttg aactacgaag tggcgaaatt cccttgggct gcttcaggtc
     6121 gtgcgattgc ttctgaatgc tcagaaggta tgaccaagtt aatttttgat aaagatactc
     6181 accgtgtatt aggtggtgcg attgttggta gcaatggtgg cgaattatta ggcgaaatcg
     6241 gtcttgcaat cgaaatgggc tgtgatgctg aagatatcgc attaaccatt catgctcacc
     6301 caacgttaca cgaatcggta ggattagcgg cagaagtatt tgaaggttca attacagact
     6361 taccaaatgc gaaagccaaa aagcgttaat cagattattt aatctctaaa aaataagcgg
     6421 tt
//
LOCUS       DANLZC010000133         6385 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.133, whole genome shotgun sequence.
ACCESSION   DANLZC010000133 DANLZC010000000
VERSION     DANLZC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.133"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            113..610
                     /gene="tpx"
                     /locus_tag="PXE44_002112"
     CDS             113..610
                     /gene="tpx"
                     /locus_tag="PXE44_002112"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDL5589583.1"
                     /translation="MSKVTLAGSPIDVAGTFPQKGDVVADFTLVNSDLSDVSLSDFDG
                     KRKVLNIFPSIDTGICATSVRVFNQKAADVANTVVLCISADLPFAQARFCGAEGIENA
                     KVLSSFRHRDLHNKLGVDIISGPLAGLTSRSVIVLDENNKVLHSELVPEIKQEPDYEA
                     ALAVL"
     gene            complement(695..2320)
                     /gene="yidC"
                     /locus_tag="PXE44_002113"
     CDS             complement(695..2320)
                     /gene="yidC"
                     /locus_tag="PXE44_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL5589584.1"
                     /translation="MNNRGLLLMGLFLVSILIFTQWQQDFDPEIQAQKQAQVAAQTQQ
                     AGDLPSASNANNPIADSITQGKTITVESDVLRLTIDTLGGDVIGSDLLKHNAELHSNT
                     PFKLLQQDGDKIYVAQSGLVGKNGIDTNAGRPQYAVTADSFKLAEGQNEISVPLTFEK
                     EGVVYTKTFTLKRGSYDVGVNFNIKNNTAQAIEVQPYGQLKHSIVESSGNLTMPTYTG
                     GAYSSSETNYKKYSFQDMEKANLSVDTKAGWIAVLQHYFVSAWVPNQDADNSLYSRTN
                     NGVATIGYRGPLTTIAPNSEANLNSKLWTGSKDQKEMGESANHLDLTVDYGWAWFIAK
                     PLFWLLTAIQKIVSNWGLAIIGVTIVVKTILYPLTKAQYTSMAKMRMLQPRITEMRER
                     FGDDRQRMSQEMMKLYREEKVNPMGGCLPILIQMPIFIALYWTFMEAVELRHAPFFGW
                     IQDLSAQDPYYILPLLMGGSMFLLQKMSPTPVADPVQQKVMTFMPVIFTVFFLWFPSG
                     LVLYWLTSNLITIAQQWLIYRNLEKKGLHSRKK"
     gene            complement(2473..2898)
                     /locus_tag="PXE44_002114"
     CDS             complement(2473..2898)
                     /locus_tag="PXE44_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HDL5589585.1"
                     /translation="MTIWKQTATCEQLNTLSQKSAVSHLGIEFTAIGEDWIEAQLQVD
                     ERTQQPFGVLHGGVSAALAETTANAGALMVCEPHQIAVGMELNISHLKSIPAGTKAIA
                     KATPLKLGREVQVWNIDIKDEQGNLCAVARLSTKTLDKR"
     gene            complement(3227..6319)
                     /locus_tag="PXE44_002115"
     CDS             complement(3227..6319)
                     /locus_tag="PXE44_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL5589586.1"
                     /translation="MIPRLSEVPQLDKATQSYLTELVNQHFSGEIASNYADRLSLATD
                     NSVYQQIPQAILFPKSVSDVVILTKLAQKPEYQHLTFTPRGGGTGTNGQSLNNNIIVD
                     LSRHMNQILELNVEERWVRVQAGVVKDQLNQFLKPHGLFFSPELSTSNRATLGGMINT
                     DASGQGSLQYGKTSDHILAIKSVLINGDILETQAVKSADFFAHIEGLNLSALGKTLHR
                     EIFNRCKTLRPKVLNELPQLNRFITGYDLKNVFNHGESEFNLTRILTGSEGSLAFICE
                     AKLNLLPIPKYRTLINIKYNSFDAALRSAPFMLEAKALSVETVDSKVLNLAKQDIVWH
                     SVSDLLTEDPANPILGINIVEYASSDQTQIETQVKALCQSLDQKMANNEAGIIGYQLT
                     SDVNSIEKIYAMRKKAVGLLGNAKGWAKPIAFVEDTAVPPENLADYIVEFRALLDRYN
                     LPYGMFGHVDAGVLHVRPALDLCDKAQVETFKQISDQVVELTAKYGGLIWGEHGKGMR
                     SYYGERFFGETLWQELRYIKTLFDPYNRLNPGKICTPLESETSLYPIDSQMRADFDRQ
                     IPIQVREEFKGAMNCNGNGLCFNFDVHSTMCPSMKVSGNRLYSPKGRATLVREWLRLL
                     TEQNVSPDDLIFKPRKQAVKLADFVAKIRNTLNKNKEYDFSHEVKAAMDTCLACKACA
                     SQCPIKIDVPTFRSQFNELYHSRYLRPLKDYVVSNLEFIAPLMAKQPRFFNFFSTSTF
                     AEKAANKTIGMTYLPALSVPNLQHQLVEVGYQGESLESLEQQATSGDFSAKNCKTLFI
                     VQDPFTSYYDAKVVADFIALIKKLGFKPIVLPFKPSGKAQHVKGFLHKFAKTAKNQAD
                     FLNRVAKLGLPMVAVDPALTLLYREEYKEILKEARGDFKVLLAHEWLREVIKNGELEN
                     CKKSANSDRLPWYLFAHCTESTALPSSMKEWQAIFTHFGETLLNENVGCCGMAGTFGH
                     ETKHVEMSKAIYQQSWQIKLKNKPLERCLATGYSCRSQVKRFEQQGIKHPIQALLDVI
                     "
BASE COUNT         1708 a         1275 c         1418 g         1984 t
ORIGIN      
        1 tatagcaaaa taaggttatt ttctaaaggc tatccacatt aaattgatag tttcctattt
       61 tgcaaaaaat cggtatattt aaaccgcttg tttatttgat aaggagaaaa aaatgagtaa
      121 agtgacttta gcaggaagcc cgattgatgt tgcaggcaca tttccacaaa aaggcgatgt
      181 ggttgccgat tttaccttag tcaacagtga tttaagtgat gtttcgcttt ccgattttga
      241 tggcaaacgc aaagtgttaa atattttccc aagtattgac accggcattt gtgccacttc
      301 tgtgcgtgtg tttaatcaaa aagcggctga tgtagcgaat acggttgtcc tttgtatttc
      361 agcggattta ccttttgccc aagcacgttt ctgtggagca gaaggaattg aaaatgccaa
      421 agtattatcc agtttccgtc atcgtgattt acacaacaaa ctgggggtag atattatttc
      481 aggtccgttg gcaggcttaa cttcacgttc agtaattgtg ttagatgaaa acaacaaggt
      541 attacacagc gaattagtgc cggaaattaa acaagagccg gattatgagg cggcgttagc
      601 ggtattataa tttgcagaat tatgctggaa aataaccgct tgtaaagcaa caaaaagccc
      661 gttgattcaa ttcaacgggc tttgttttta gtgattattt cttacgagag tgtaagcctt
      721 ttttctcaag gttacggtaa atcaaccatt gctgtgcaat cgtgattaag tttgaggtta
      781 accagtaaag cactaagcct gatgggaacc ataagaagaa cacggtaaag ataaccggca
      841 taaaggtcat tactttttgt tgtaccgggt cagctaccgg tgttggcgac attttttgta
      901 acaagaacat cgaaccaccc attaataaag gcaaaatgta gtatgggtct tgtgccgata
      961 agtcttgaat ccagccgaag aaaggtgcgt ggcgtaattc taccgcttcc ataaatgtcc
     1021 aatataaagc aatgaaaatc ggcatttgga ttaaaattgg taagcagcca cccattgggt
     1081 tcactttttc ttctctgtag agtttcatca tctcttggct catacgttgg cggtcgtcgc
     1141 cgaaacgttc acgcatttca gtaatgcgag gttgtaacat acgcattttt gccatcgagg
     1201 tgtattgtgc tttggttaat gggtaaagaa tggttttcac tacaatagtg acaccgataa
     1261 ttgccaagcc ccagttgctt acgatttttt gaattgcggt tagtaaccag aaaagcggtt
     1321 tagcgataaa ccacgcccag ccgtaatcta cggttaagtc taggtgattt gctgattcgc
     1381 ccatttcttt ctgatcttta gaacctgtcc aaagtttgct atttaaatta gcctctgaat
     1441 ttggcgcaat cgtagttaaa gggccacggt aaccgatagt tgctacccca ttattggtgc
     1501 gtgaataaag gctgttatcc gcatcttggt ttggcaccca agcagacacg aaatagtgct
     1561 gtaataccgc aatccaaccc gctttggtgt ccacgcttaa gttggctttt tccatatctt
     1621 ggaaactgta ttttttgtag ttggtttctg aagaagaata agccccgccg gtgtaagtcg
     1681 gcatggttaa attgccggaa ctttcaacta tagagtgttt taattgcccg taaggctgaa
     1741 cttcaatcgc ttgtgcagta ttatttttga tattgaagtt taccccaaca tcgtagctac
     1801 cacgttttag cgtgaaagtt ttggtgtaaa ccacaccctc tttttcaaag gttaatggta
     1861 cagaaatttc attttgccct tcggctaatt tgaagctatc tgccgttacc gcatattgtg
     1921 ggcgaccggc attggtatca atcccgtttt tacccactaa accgctttga gcaacgtaga
     1981 ttttgtcgcc gtcttgctgt aatagtttaa atggcgtgtt agagtgtaat tcagcattgt
     2041 gttttaacaa atctgagccg atgacatcac cgcctaaggt atcaatagtc agacgtaata
     2101 catcgctttc cacggtaatg gttttacctt gcgtgatgct atctgcgatt ggattattgg
     2161 cattggaagc agacggaaga tcacccgctt gttgggtttg tgctgccact tgagcttgtt
     2221 tttgtgcttg aatttcagga tcaaaatctt gttgccattg tgtaaaaatc aaaatagaca
     2281 caagaaataa acccattagc agtaagccac gattattcat gttggaattc tcttgttagt
     2341 aatggataaa aagaaaggta ttttataata ggttagcttg ttttccaaac gccttttatg
     2401 aacaagcggt cggattttcc aagttttttg caaaattttc ggagaatttg accgcttgtg
     2461 gagaataaga aactagcgtt tatctaaggt tttggtggaa aggcgagcaa ctgcacacag
     2521 gttaccttgt tcgtctttaa tgtcgatatt ccaaacctgt acttcacgcc ctaatttcag
     2581 cggtgtagct ttggcaatcg cttttgtgcc tgccggaatc gattttaaat ggctgatgtt
     2641 tagctccatt cccaccgcaa tttgatgggg ttcacacacc attaacgccc ctgcattggc
     2701 agtagtttcc gcgagtgctg ccgatacacc gccgtgcaat acgccaaacg gctgttgagt
     2761 gcgttcgtcc acctgaagct gtgcttcaat ccaatcttca ccgatagcgg taaattcaat
     2821 ccctaagtgt gacaccgccg atttttggct gagtgtgttt aattgttcac aagttgctgt
     2881 ttgcttccaa atggtcatta ttatttcctg tctaataaaa aagctgtcta tttagacagc
     2941 tttcattatc tctttgagcg aaacatacta cgccaatagt acattgtttg gtcgcccata
     3001 ttaccttaag ccatatagca ctaaggaggt gatttatgtg agaaaatcag aagttttctt
     3061 tctggttgcg ttgctaatat tattagcaac ttccgctagc attaaagtac tcaccatact
     3121 tgatgtgcta gtttggttat tcgctagata acctaaaatc agacagaggc aggctggtta
     3181 cctgcctcgc tttttattct aagtactttt aggttacatt tcaaccttaa atcacgtcca
     3241 gcaacgcttg aattgggtgt ttaatgcctt gttgttcaaa acgtttgact tgcgagcggc
     3301 aggagtagcc ggttgctagg caacgctcaa gcggtttatt ttttaattta atttgccatg
     3361 attgctgata aatcgctttc gacatttcaa cgtgtttggt ttcgtgtccg aacgtgcctg
     3421 ccattccaca gcaaccgaca ttttcattga gtaaggtttc accaaaatgg gtgaaaatag
     3481 cttgccactc tttcattgag cttggcaagg cggtggattc ggtgcagtga gcaaataaat
     3541 accacggcaa gcggtcggaa tttgccgatt ttttgcaatt ttccagctcg ccgtttttaa
     3601 tcacttccct taaccattcg tgtgccaata agactttgaa atccccccga gcttctttga
     3661 ggatctcttt gtattcttca cgataaagca aagttaaggc aggatcgaca gccaccatcg
     3721 gtagccccaa tttcgccaca cggtttaaaa aatcggcttg gtttttcgct gttttcgcaa
     3781 atttatgcaa aaagccttta acgtgttgtg ctttaccgct tggcttgaat ggcaacacaa
     3841 tcggcttaaa gcctaatttt ttaatgagag cgataaagtc tgccaccact ttggcatcgt
     3901 aataagaggt aaacggatct tgcacaataa acaaggtttt gcaatttttt gcagaaaaat
     3961 caccgcttgt agcttgttgt tcaaggcttt ctaagctctc gccttggtag ccgacttcca
     4021 ccaactgatg ttgcagattt ggcactgaca atgccggcaa ataggtcatt ccaatggttt
     4081 tgtttgcagc tttttcggca aaagttgagg tgctgaaaaa gttaaaaaaa cgtggttgtt
     4141 tcgccattaa aggggcgata aattctaagt ttgagaccac ataatctttc agtggtcgca
     4201 aatagcggct gtggtaaagc tcgttaaatt gcgatctgaa cgtcggtacg tcaattttga
     4261 ttgggcattg gctggcacag gctttgcagg caaggcaggt gtccatcgcc gctttgactt
     4321 cgtgtgaaaa atcatactct ttgtttttat tgagcgtgtt gcgaattttt gcaacaaaat
     4381 cagctaattt gaccgcttgt ttgcgaggtt tgaagatcaa atcgtccggc gaaacgtttt
     4441 gttcggtaag taatctgagc cattcacgca caagagtagc ccgccctttc ggtgagtaaa
     4501 gacggttgcc cgagactttc attgaagggc acatcgtgct gtgtacatca aagttaaaac
     4561 atagcccgtt gccgttacaa ttcattgctc ctttaaattc ttctcgcact tggattggaa
     4621 tttggcggtc aaaatcggct cgcatttgtg agtcaatcgg gtagagcgag gtttcgcttt
     4681 ctaatggtgt acaaatttta cctgggttca agcgattgta cgggtcaaat aaggttttga
     4741 tgtaacggag ttcttgccac aaagtttcgc caaagaaacg ttcgccgtag taagaacgca
     4801 tacctttgcc gtgttcgccc caaatcaagc caccgtattt ggcggtgagt tcgactactt
     4861 ggtcggaaat ttgcttaaag gtttccactt gggctttgtc gcataagtca agagcagggc
     4921 gaacgtgtag cacgccggca tcaacgtggc cgaacattcc gtaaggcaga ttatagcgat
     4981 ctaataaagc tcgaaattcc acgatgtaat cggcaaggtt ttccggtggc acagcggtgt
     5041 cttctacaaa ggcgatcggt tttgcccagc ctttggcatt gcctagcagg cctacggctt
     5101 ttttccgcat tgcgtagatt ttctcgatag aatttacatc actcgtgagt tggtagccga
     5161 taatcccggc ttcattattt gccatttttt gatcaaggct ttggcagagt gctttcactt
     5221 gggtttcaat ttgagtctga tcgcttgagg catattcaac aatgttaatc cctaaaatcg
     5281 ggttggctgg gtcttcggtt aataaatctg acaccgaatg ccagacgata tcttgcttgg
     5341 caagattgag cactttggaa tcaaccgttt ccaccgataa ggcttttgcc tctaacataa
     5401 aaggagcgga gcgtaaggcg gcatcaaagg aattatattt aatattaatt aaggtgcgat
     5461 atttgggaat cggcagtaaa ttcagtttgg cttcgcaaat aaaggcaagc gaaccttcag
     5521 aaccggttaa aatacgggta aggttaaatt cgctttcacc atggttaaac acatttttta
     5581 aatcatagcc ggtaataaaa cggttaagct gaggcagttc gttcagcact ttggggcgta
     5641 atgttttgca acggttgaaa atttcacggt gcaaggtttt gccgagagcg gagagattta
     5701 acccttcaat atgtgcaaaa aaatcggcag atttgaccgc ttgtgtctct aaaatatcgc
     5761 cgttaataag cacagatttg atggcaagaa tatgatcaga tgttttgcca tattgcaaag
     5821 aaccttgccc agaggcatcg gtgttgatca ttccgcctaa agtcgctcgg ttactggtag
     5881 aaagttcggg ggaaaagaaa agtccatgtg gttttaaaaa ttgattgagc tggtctttca
     5941 ccacgccagc ctgcactcga acccaacgct cttccacatt cagttccaaa atctgattca
     6001 tatggcggga gagatcaaca ataatattat tgttaagaga ttgcccgtta gtgcctgtgc
     6061 caccgcctcg tggagtaaaa gttaaatgtt gatattctgg cttttgggca agtttggtta
     6121 gaattacgac atcagaaacg gatttcggaa acagaatagc ttgcggaatt tgctgataca
     6181 cgctgttgtc tgttgccagt gaaagcctat ctgcataatt tgatgcaatt tccccactaa
     6241 aatgctgatt gacgagttca gttaaatagg attgggtggc tttatccagt tgcggtactt
     6301 cagagagtct aggtatcata agctgtgttt ggttttttat aataaaagca ctaaattttg
     6361 cttatcttag caatttttct aaaaa
//
LOCUS       DANLZC010000134         6353 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.134, whole genome shotgun sequence.
ACCESSION   DANLZC010000134 DANLZC010000000
VERSION     DANLZC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.134"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..343)
                     /locus_tag="PXE44_002116"
     CDS             complement(<1..343)
                     /locus_tag="PXE44_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5589587.1"
                     /translation="MNDLIIYNTEDGKSNVALLVVENEAWLTQNQLAELFDTSVQNIS
                     EHIKNILKDKELDENSVIKDYLITAQDGKNYQVKHYSLEMILAIGFRVRSPRAVQFRK
                     WANSTLRTYLEK"
     gene            complement(503..1867)
                     /gene="mpl"
                     /locus_tag="PXE44_002117"
     CDS             complement(503..1867)
                     /gene="mpl"
                     /locus_tag="PXE44_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDL5589588.1"
                     /translation="MKKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFL
                     AAHGIEIIPNYDVAQLQPAPDLVVIGNAMSRGNPCVEYVLDNQLNYTSGPQWLHDHLL
                     KDKWVLAVSGTHGKTTTTGMLAWILDQNGIDTGFLIGGVAGNFGISARAGNSPFFVIE
                     ADEYDSAFFDKRSKFVHYTPKTLIINNMEFDHADIFDDLKAIQRQFHHLVRTMPQSGC
                     ILSAIADQNVQDTLKMGSYSQLQFIGEQNEWFAKPLNADCSQFEVFHLGVKQGEVHWS
                     IIGKHNMHNALMAIAAAHHAGVEVQGACEALGKFVNANRRLEVKGEVNGITVYDDFAH
                     HPTAIEATIDALRGKVGKNQRILAVLEPRSNTMKMGVHKDEIAPSLHDAEAVFVYQPD
                     IIAWSVSEITNVLSQPAYWSANIDDLVDLVVKEAKPTDHILVMSNGAFGGIHQKLLAK
                     LADK"
     gene            complement(2163..2918)
                     /locus_tag="PXE44_002118"
     CDS             complement(2163..2918)
                     /locus_tag="PXE44_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HDL5589589.1"
                     /translation="MKLKSFKPMFMLLSVAFLTACSSSINPETGVRDDPLEGFNRAMW
                     KVNYDYLDPYVLKPVAKGWKEYVPSPVKTGLINVANNLDEPVSFVNRLIEGEGKKAMV
                     HFNRFWINTVFGLGGLIDWASLDEGLRLNNGQRGLGESLGAHGVPSGSYIMMPAYGAT
                     TPRQGIGSAVETGYSVLTYVGSPWSIAKFVVQGVDTRSKLLEQDALLEQAQDPYVTFR
                     EAYFQNLEFKVKDGNVEQSQQEQLSEEELKNID"
     gene            3073..3390
                     /gene="metJ"
                     /locus_tag="PXE44_002119"
     CDS             3073..3390
                     /gene="metJ"
                     /locus_tag="PXE44_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDL5589590.1"
                     /translation="MADWNGEYISPYAEHGKKSEQVKKVTVSIPIKVLEILTNERTRR
                     QIKNLRHATNSELLCEAFLHAFTGQPLPNDEDLMKDRADEIPEAAKQKMCELGIDPDE
                     WQY"
     gene            3438..3668
                     /locus_tag="PXE44_002120"
     CDS             3438..3668
                     /locus_tag="PXE44_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF997 family protein"
                     /protein_id="HDL5589591.1"
                     /translation="MYQQLTTEARWAVLLTLIYLLGWVGFAYFSPQGRGIFGFPIWFE
                     LSCIFLPVLFSLVATIVVKKVYKNIDLEVNNE"
     gene            3661..5085
                     /gene="panF"
                     /locus_tag="PXE44_002121"
     CDS             3661..5085
                     /gene="panF"
                     /locus_tag="PXE44_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDL5589592.1"
                     /translation="MNSEMILPLIAYLIFVFGVAFYAYRKRQGGSFISEYYIGGRSMS
                     GFVLAMTTAATYVGASSFIGGPGAAYKYGLGWVLLAMIQVPAVLLSLGALGKKFALLA
                     RKHNSVTINDMLLARYQNKFVVWIAGLALLLSFFAMMTVQFIGAGRLLETTLGIPYQT
                     AVMIFAITVGLYTFIGGFRAVVLTDTIQGLVMIVGTSILLAGVIYATGGVENAMHTLE
                     AINPQLTTPYGIDERPLDFTFMTSFWVLVCFGLIGLPHIAVRSMACKNSQALHRAMII
                     GTVVIAILMFGMHLSGVLGRAVVPDLKVPDQVIPTLMVQVLPPVVAGIFLAAPMAAIM
                     SSIDSMLIQSSSTLIKDLYLAIKPNAINNEKKIKLFSTITTLTFTVLLVFAALTPPEM
                     LIWLNLLSLGGLEATFLWVIVLGLYWEKANATGAIYSMLAGLSCYVLMTTFKIAIFSF
                     HAIVPSLLIGLIAFLIGNRFAPKV"
     gene            complement(5162..6352)
                     /gene="nhaA"
                     /locus_tag="PXE44_002122"
     CDS             complement(5162..6352)
                     /gene="nhaA"
                     /locus_tag="PXE44_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDL5589593.1"
                     /translation="MEKLKEFLKSEASGGVLLLVFAFAAIVCANTSLSDFYFEFLQTI
                     VSVQFGEFALNKPLLLWVNDGLMAVFFTLVGLEVKKELFEGSLSSYKKAIFPAIAAVG
                     GMIVPAIIYWAINQNHPEYHNGWAIPMATDIAFAVGIVALLGTRVPLALKAFLLALAI
                     IDDLGAIVVIAIFYSQEISVQALTIAAIAIIGLVLLNRFKVTSLCAYLVVGLVLWTSV
                     LKSGVHATLAGVIIGFCVPLKGKHGERPAEELEHILAPWCAYMILPIFAFANAGVYLL
                     EMGMEQLSSPLPLGIALGLIIGKTLGVFGFSYLAVLFRLASLPQGVNFKQIFAIAVLC
                     GIGFTMSMFLASLAFDVSEAGESITALARLGILLGSAVSAILGYTLLRMATKQNNPEN
                     NATY"
BASE COUNT         1817 a         1422 c         1277 g         1837 t
ORIGIN      
        1 ctttttcgag ataagtgcgt aatgtgctgt ttgcccattt tctaaactgt actgctcttg
       61 ggctacgaac tcgaaaccct atcgccagaa tcatttcaag tgaataatgt tttacttgat
      121 aattcttgcc atcttgagca gttatcaagt aatccttgat aactgaattc tcatctaact
      181 ctttgtcttt taagatattt ttaatgtgtt cggatatatt ttgcacagag gtgtcaaaaa
      241 gttctgcaag ttgattttgt gtcagccaag cttcattttc aaccactaac aaggctacat
      301 tgcttttgcc gtcttccgta ttataaataa ttaaatcgtt catattttat tctgtttatt
      361 ggtagagata ttagtcgcta ttttcaataa aacatgtata aatatccaga attgattttg
      421 aatttttcga tgcagatcac aaaaatagag agttatacaa acaagcggta aaattttgta
      481 aaaaatttac cgcttgtaaa ggttacttat cagctaattt ggcaagtagt ttttgatgaa
      541 taccgccaaa ggctccgttg ctcatcacta agatgtgatc ggtgggtttg gcttctttga
      601 cgactagatc gactaaatca tcaatatttg ccgaccaata cgccggttgc gataacacat
      661 tggtaatctc gctcacactc cacgcaataa tatcaggctg gtaaacaaac actgcttcgg
      721 catcgtgtag gcttggggct atttcatctt tatggacacc cattttcatt gtatttgaac
      781 gaggctctaa cactgccaaa atgcgttgat ttttgccgac tttaccacgc agagcatcaa
      841 tagtcgcttc tatcgcagtt gggtggtgag cgaaatcatc ataaacggta atgccgttta
      901 cctcgccttt cacttccaaa cggcggttag cattcacaaa cttgcctaat gcctcgcaag
      961 ccccttgcac ttcaacccct gcgtgatgag cggcggcaat cgccatcaga gcattatgca
     1021 tattgtgctt gccgataatc gaccaatgca cctcgccttg tttaacgcct aagtgaaaca
     1081 cctcaaactg tgagcaatcg gcatttaacg gtttagcaaa ccattcgttc tgttcgccaa
     1141 taaattgaag ctggctgtaa ctgcccattt ttagtgtatc ttgcacgttt tgatcagcga
     1201 ttgccgacaa aatacagcca ctttgtggca tcgtgcgaac aaggtggtgg aactggcgtt
     1261 gaatcgcttt taaatcatca aaaatatcgg cgtggtcaaa ttccatatta ttgataatca
     1321 gcgttttcgg ggtgtaatgc acaaatttag aacgcttatc aaagaacgca gaatcgtact
     1381 cgtccgcctc aatcacgaag aatggactgt taccggcacg agccgaaatg ccgaaattac
     1441 cggcaacacc gccaattaaa aagccggtat caatcccatt ttggtctaaa atccacgcta
     1501 acattcctgt tgtggtagtt ttgccgtgcg tgccggaaac cgccagcacc catttgtctt
     1561 taagcaaatg atcgtgcaac cattgcgggc cggaggtgta attgagttgg ttgtccaaca
     1621 catattccac gcacggatta ccacggctca tcgcattacc aatcaccact aaatcaggag
     1681 ccggttgcag ttgagcaaca tcgtaattcg ggataatttc aataccgtga gcagctaaaa
     1741 aggtactcat cggtgggtaa acattagtgt ctgagccggt cactttatag ccaagctcac
     1801 gggcaatcat tgccacaccg cccataaagg tgccgcaaat gcctaaaata tggatatgtt
     1861 ttttcatctt tttatattta aaatgttaga atcatcccct ctccccttgt gggagaggga
     1921 tagatttttt cttcgtgcag aagaaaaaat cagggagagg ggtaagcggt catttttagg
     1981 cttaatattg taagctccct ctctctgaaa aatatttctg aacgaaatat ttttcggtct
     2041 caatactcgc tacgctcgct cctgtggagc cgttggcaaa gccaacgttc aaatgtaaac
     2101 atttgtccca caaggggaga ggtgcgcttt agatgaggcg taccatccct cgttcacatc
     2161 aattaatcaa tattcttcaa ctcttcttcc gaaagctgct cttgttggct ttgctcaaca
     2221 ttgccatctt tcactttaaa ttcaaggttt tggaaataag cttcacggaa ggtaacataa
     2281 gggtcttgag cttgctctaa caacgcatct tgttctaata atttagaacg ggtatctacc
     2341 ccttgtacca caaatttagc gatagaccaa ggtgacccaa cataagtcag caccgaataa
     2401 ccggtttcca ccgcactacc aataccttga cgaggtgtgg ttgctccata agccggcatc
     2461 ataatatagg agccggaagg cacgccgtga gcgcctaaac tctcacctaa accacgttgt
     2521 ccgttattta aacgtaaccc ctcatctaaa ctcgcccagt caattaaccc acctaaacca
     2581 aacacagtgt tgatccagaa gcggttgaag tgaaccatcg cttttttacc ttctccttca
     2641 attaagcggt ttacaaagct caccggctca tcaaggttat tagcaacatt aattaagcct
     2701 gttttaaccg ggcttggtac atactctttc caacctttag ctaccggttt taacacataa
     2761 gggtctaaat agtcgtaatt gactttccac atcgcacggt taaacccctc aagtgggtcg
     2821 tctcgcacgc cggtttctgg gttaatgctt gatgaacaag cggttaaaaa tgccacactg
     2881 agtagcataa acatcggttt gaacgacttg agtttcataa attgaacctt ttgcagagaa
     2941 atttgtccat tgtagcgaag ttgataaaaa atacaaaaca aaaagccgtt ttgcctctag
     3001 aaatctagac gtctagattg agatatagtt cgcttttgat tacaatacag gcaacattta
     3061 aggagcaaac aaatggcaga ctggaacggc gaatatatta gcccttatgc cgaacacggc
     3121 aaaaaaagtg aacaagtcaa aaaagtgacg gtatccattc caattaaagt attggaaatt
     3181 ttaactaatg agcgaacccg tcgccaaatc aaaaatcttc gtcacgcaac caacagcgaa
     3241 ttattgtgcg aggctttttt gcacgccttt accggtcagc ctttgccgaa tgatgaagac
     3301 ttgatgaaag acagggcaga tgaaatccct gaagcggcaa aacaaaaaat gtgtgagctg
     3361 ggaatagacc cggacgaatg gcaatattag acacaccttg caactgcgag gttcttttta
     3421 tgttttaagg agactttatg tatcaacaat taacaacaga agcacgttgg gctgtgttgc
     3481 ttacgctgat ttatctgctc ggctgggtag gttttgctta cttttcaccg caaggcagag
     3541 gcattttcgg ctttccgatt tggtttgaac tttcttgcat tttcttaccc gtactattta
     3601 gcttagttgc caccattgtc gtcaaaaaag tgtataaaaa tatcgatctt gaggtgaata
     3661 atgaatagtg aaatgatttt acccctgatt gcttatttaa tttttgtgtt tggcgtagcg
     3721 ttttatgctt accgtaaacg ccaaggcgga agctttattt cggaatacta tatcggtggg
     3781 cgttcaatgt cgggctttgt gttagcaatg acaaccgccg ccacttatgt gggggcaagt
     3841 tcctttatcg gtgggccggg tgctgcctat aaatacggtt taggctgggt gttactggca
     3901 atgattcaag tgcccgcagt gctactctct cttggagcat taggcaaaaa atttgcctta
     3961 cttgcccgca agcataatag tgtcaccatt aacgatatgt tattagcccg ttaccaaaac
     4021 aaatttgtgg tttggattgc aggactagcc ttattacttt cgttttttgc gatgatgacg
     4081 gtgcaattta tcggtgccgg tcgcttactt gaaaccacac tcggaatccc ttaccaaaca
     4141 gcggtaatga tttttgccat cacagtcggg ctttatacct ttatcggcgg ttttagagcg
     4201 gtggtgctaa ccgatactat tcaaggttta gtaatgatag tcggcacaag cattttactc
     4261 gctggtgtga tttatgccac aggcggagtg gaaaatgcaa tgcacaccct tgaagccatt
     4321 aacccacaac ttaccacccc ttacggcatt gacgaacgcc cgcttgattt cacctttatg
     4381 acttcatttt gggtattggt ttgctttggt ttaatcggct taccgcatat tgcggtcaga
     4441 agtatggcat gcaaaaacag ccaagccttg caccgagcaa tgattatcgg cacggtagtc
     4501 attgcaattc taatgtttgg aatgcacctt tcaggcgtat taggcagagc ggttgtgcct
     4561 gatctcaaag tgccggatca agtcattcca acactaatgg tacaagtgct accaccggtg
     4621 gttgccggta ttttccttgc cgccccaatg gcagcgatta tgtcttcgat tgactcaatg
     4681 ttgattcagt cgtcttcaac cttaattaaa gatctctatc tggcaattaa gccgaatgcc
     4741 attaataacg agaaaaaaat caaattgttc tccactatta ctactcttac ttttacggta
     4801 ttactcgtgt ttgccgccct aaccccgcct gagatgttaa tttggctgaa cctactctct
     4861 ttaggtggtt tagaagcgac tttcctatgg gtaattgtac tagggttgta ttgggaaaag
     4921 gcaaatgcca ccggtgctat ttactcaatg ctggcaggct taagttgtta tgtattaatg
     4981 actaccttta agattgctat ttttagcttc cacgccattg tgccttcact gctaattggt
     5041 ttgattgctt tcttaatcgg caaccgattt gctcctaagg tgtaaaataa aataggcata
     5101 gtggtatgga agttcactat gcctattttg caaaatttcc gctcaattca accgcttgta
     5161 gttaataagt ggcattattt tctggattat tctgcttagt cgccattcta agtaacgtat
     5221 aacctaaaat cgctgaaacc gcagagccga gtaaaatacc taatcgagct agagcggtaa
     5281 tactttcccc agcctcactt acatcaaagg caagacttgc taagaacatc gacattgtaa
     5341 aaccgatacc gcatagcacc gcaatggcaa aaatttgctt aaaattaacc ccttgcggca
     5401 ggctggcaag gcggaataat actgctaaat aactaaaacc gaatacacct aaggttttgc
     5461 cgataattaa gccgagcgca atacctaacg gcaatggtga tgagagttgc tccattccca
     5521 tttctaataa gtaaacgccg gcattagcga aggcgaagat cggtaaaatc atataagcac
     5581 accaaggggc aagaatatgt tctagctcct ctgccggtcg ttccccatgt ttacctttca
     5641 gcggtacgca gaagccgatg atcacgccgg caagggtagc gtgaacccct gattttaaaa
     5701 cggaagtcca tagcactaag cctactacca aataagcaca gagtgaggtc actttaaagc
     5761 ggttaagtag cactaagccg ataatcgcaa tcgcggcaat agttaaggct tgcacactga
     5821 tttcttgcga atagaaaatg gcgattacca caatcgctcc tagatcatca ataatcgcta
     5881 atgccaacaa aaatgccttt aaagccaatg gcacacgagt gcctaataac gccacaatgc
     5941 ccaccgcaaa ggcaatatca gttgccattg gaattgccca accattatga tattcagggt
     6001 ggttttgatt aatcgcccaa taaataatcg ctggaacaat cataccacct actgctgcta
     6061 tcgcaggaaa aatagctttc ttataacttg aaagcgaacc ttcaaacaac tcttttttta
     6121 cttccagccc caccaacgta aaaaacactg ccattaagcc atcgttaacc cagagtaata
     6181 gcggtttatt caaggcaaat tcaccaaact gtacactaac aatcgtttgt aaaaactcaa
     6241 aatagaaatc acttaaggag gtgttggcgc acacaattgc tgcaaaagca aaaaccagaa
     6301 gcaatacgcc tcctgaggct tcagatttta aaaattcttt taatttttcc act
//
LOCUS       DANLZC010000135         6221 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.135, whole genome shotgun sequence.
ACCESSION   DANLZC010000135 DANLZC010000000
VERSION     DANLZC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.135"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(27..425)
                     /gene="mutT"
                     /locus_tag="PXE44_002123"
     CDS             complement(27..425)
                     /gene="mutT"
                     /locus_tag="PXE44_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDL5589594.1"
                     /translation="MSKPIIQVSAGIIRNEFGQIYLTQRLEGQDFAQSLEFPGGKVDP
                     NETPEEALKRELEEEIGIQVLSAFPYENFCFEYPTKIIEFFFYLVEEWVNEPYGREGQ
                     EGFWIAQSDLDEGAFPPANAQLIKRLKEHA"
     gene            complement(512..3238)
                     /gene="secA"
                     /locus_tag="PXE44_002124"
     CDS             complement(512..3238)
                     /gene="secA"
                     /locus_tag="PXE44_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL5589595.1"
                     /translation="MISKLLTSIFGSSNDRTLKRLRKRVAQINKLEPTFEQLSDEALK
                     DKTAEFKQRLADGVSLDSLLPEAFATVREASKRVLGMRPFDVQLIGGMVLTGRNIAEM
                     RTGEGKTLTATLSCYLNALTGKGVHVVTVNDYLARRDAETNRPLFEFLGLTVGVNIPG
                     LPSDAKRAAYQADITYSTNSELGFDYLRDNLAHDKNERFQRELHYALVDEVDSILIDE
                     ARTPLIISGPAEDATQIYQAVDQIIPHLIAQDKEDSEEYTGEGDFTLDLKNKQAHLTE
                     RGMVKVEDFLTQMGLMEEGESLYHPARISLLHHVSAALRAHKLFERNVDYIVKDGEIV
                     IIDEHTGRTMAGRRWSDGLHQAIEAKERVAIQGENQTVASITYQNYFRLYEKLAGMTG
                     TADTEAFEFQQIYGLNTVVIPTNRPVIRDDRTDLMFKSEAEKFAAIITEIRDCIERKQ
                     PVLVGTASVEKSELLSAELTKAGIAHNVLNAKFHAQEAEIVAEAGAPGAVTIATNMAG
                     RGTDIVLGGNWKMEVAKLENPTQEQIDAIKAAWQERYDIVMQAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDPGSSRFYLSLDDALMRIYLNEGKLNMMRKAFTEEGEAMESKLLT
                     KVIASAQAKVEAHNFDGRKHLLQYDDVANEQRKVIYEQRNHLLETDNIASMIESIRQD
                     VFESTINQYIPPQSIEEMWNVPGLEERLRREFGLDLPISHWLETEKDLHEETLRERIL
                     NIAIAEYNAKEEIVGAEVMRNFEKGVMLQNLDELWKEHLASMDYLRKGIHLRGYAQKD
                     PKQEYKKESFAMFTNMLDTLKLNVISILSRIQVRSKEEVEEAQRQQAEMDAASTPVTQ
                     ATDSAAGSREENSAESLSEEELANLNIGRNDPCPCGSGKKYKHCHGNKARYS"
     gene            complement(3297..3617)
                     /locus_tag="PXE44_002125"
     CDS             complement(3297..3617)
                     /locus_tag="PXE44_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2547 family protein"
                     /protein_id="HDL5589596.1"
                     /translation="MSLFLKHFHRTPFWSQLFLGMVAILALPEIQVAYANESEQETVL
                     NQSFLQYAQSANDIEQQSLFIAELIHKIVEIKPQAVHFCEFFANSYRLDRQNAYPIRA
                     GPQT"
     gene            3738..4040
                     /locus_tag="PXE44_002126"
     CDS             3738..4040
                     /locus_tag="PXE44_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HDL5589597.1"
                     /translation="MKNSAPKKINDILQNSSLSRIVEKANFINDLNQKIQKRWPESYR
                     GLYRVVNLVDNQLIVSVKNATVRQGLILQQNMLLKQIQTDLPEITQLEFRVNPSVT"
     gene            4065..4439
                     /locus_tag="PXE44_002127"
     CDS             4065..4439
                     /locus_tag="PXE44_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HDL5589598.1"
                     /translation="MKKIALIGFTTAMLGACSLLPQKSVSGTYAGELPCADCEKIQAQ
                     LTLNADKTYQYDTVYFKDKKEYTYRDKGTYSWEPNKPNVIRLEKASGNLAFQVSEQHV
                     ELCDPNGNTVKNSNNYKLGKVR"
     gene            complement(4615..4704)
                     /locus_tag="PXE44_002128"
     tRNA            complement(4615..4704)
                     /locus_tag="PXE44_002128"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4668..4670),aa:Ser,seq:gga)
     gene            complement(4796..5107)
                     /locus_tag="PXE44_002129"
     CDS             complement(4796..5107)
                     /locus_tag="PXE44_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL5589599.1"
                     /translation="MSVEQTIIQKIKTEFQPIFFNVENESYMHSSGRGAESHFKLTLV
                     SDKFEGQRTVARHRAVYACLAEELANGVHALALHLYTQGEWEATGGLVPKSLNCLGVG
                     Q"
     gene            5199..5786
                     /locus_tag="PXE44_002130"
     CDS             5199..5786
                     /locus_tag="PXE44_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589600.1"
                     /translation="MKRSKKTLVLATTFVAALLAGCQSQSNTLTFSTPTPTAVFNTNN
                     QTAAVNVMTQDLRTSREIASYTKNGEVIRLTAVPEVAAMFQQAVQQDLNAKGFQIVQG
                     AANANVVVNIRKFFATVEQGNLRYKADAQVSVKVAVQGSKGNFSKNFNATRGYEGAFG
                     ASNDEIKKVLDQAYSDVVKAIYNDNEIANAIHQFK"
     gene            5958..6131
                     /locus_tag="PXE44_002131"
     CDS             5958..6131
                     /locus_tag="PXE44_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589601.1"
                     /translation="MNNPYHNVSQEYLDYLAKKVKQGKEDIKNGRIYSPEEVRKRVIA
                     AAFRGAENAKKVA"
BASE COUNT         1917 a         1321 c         1139 g         1844 t
ORIGIN      
        1 actttagata cagttctcaa tacaaattat gcgtgttctt ttaagcgttt aattaattgg
       61 gcatttgccg gtgggaaagc tccctcatct aaatccgatt gtgcgatcca aaaaccttct
      121 tgcccttctc ttccgtaagg ttcatttacc cactcttcca ccaaataaaa gaaaaattca
      181 atgatttttg ttggatattc aaagcaaaaa ttttcatacg gaaaagcgct taacacttga
      241 ataccaattt cttcttctaa ttctcgtttt agtgcttctt caggggtttc attgggatcg
      301 actttccccc cgggaaattc aagagactga gcaaaatctt gcccttctaa acgttgggtt
      361 aagtaaattt gcccaaattc gttacgaata atccctgctg agacttgaat aatcggtttt
      421 gacatagttt ccttcactct ttctataaat tcaagcggtc atattaagca gaaaatttgc
      481 aaattcctga ctgaatatga ccgcttgtta attacgaata tctcgctttg ttaccatggc
      541 aatgcttata ttttttgccc gaaccacaag ggcaagggtc atttcgacca atatttaagt
      601 ttgccaactc ttcttcactt aagctctctg cgctattttc ttctctacta ccagcagcag
      661 aatcggtagc ttgtgtaact ggcgtacttg cagcgtccat ttcagcttgc tggcgttgcg
      721 cttcttccac ctcttctttg ctacgcactt gaatacggct taaaatgcta attacattca
      781 attttaaggt atcaagcata ttggtaaaca tcgcaaaaga ttccttttta tactcttgct
      841 tcggatcttt ttgtgcataa ccacgcaagt gaataccttt gcgtaaataa tccattgagg
      901 ctagatgctc tttccaaagc tcatctaagt tttgtagcat tacacctttt tcaaaattac
      961 gcattacctc tgcgcctact atttcttcct tcgcattgta ctctgcaatc gcaatattta
     1021 agatacgttc acgcaaagtc tcttcgtgca agtctttttc agtttcaagc cagtgagaaa
     1081 tcggtaaatc caacccaaat tcacggcgta aacgctcttc taagcccggc acattccaca
     1141 tttcctcgat agattgaggc ggaatatatt ggttaatcgt gctttcaaat acatcttggc
     1201 gaatgctttc gatcattgag gcaatattat ctgtttctaa tagatgattg cgttgttcgt
     1261 aaattacttt acgctgttca ttggcaacat catcatattg taataaatgc ttacgcccat
     1321 caaagttatg cgcttctact ttcgcctgtg cagaggcaat cactttggtt aacagtttag
     1381 actccatcgc ttcaccttct tcggtaaagg ctttacgcat catatttaat ttaccttcat
     1441 tgaggtaaat tctcattagg gcatcatcta aagagagata gaaacgtgat gaccccgggt
     1501 caccttgacg accagaacga cctcgcaact ggttgtcgat acggcgggat tcgtggcgtt
     1561 ctgtaccaat aatatgtaaa ccaccggctt gcattacgat gtcataacgc tcttgccacg
     1621 cagctttaat cgcatcaatt tgttcttgtg tcgggttctc aagcttggct acttccattt
     1681 tccagttacc gccaagcaca atatccgtac cacgaccagc catattggtt gcgatagtaa
     1741 ccgcccccgg agctcccgct tccgctacaa tttcagcctc ttgagcgtgg aatttcgcat
     1801 ttaatacgtt atgagcaata ccggctttgg ttaattctgc cgataataac tctgattttt
     1861 ccaccgatgc tgtgccaacc aaaacaggct gtttacgctc gatacagtct ctaatttcag
     1921 taataatcgc cgcaaatttc tccgcttcgc ttttaaacat taagtctgta cggtcatcac
     1981 ggatcaccgg tctgttggtt ggaatcacca ccgtatttaa gccataaatt tgctggaatt
     2041 caaaggcttc ggtgtctgcc gtaccagtca taccggctaa tttttcgtac aaacggaaat
     2101 agttttggta agtaattgag gcaacagttt ggttttcgcc ctgaatagcc acacgctctt
     2161 tagcttcaat cgcttggtgt aagccgtcag accaacgacg tccagccatt gtacgaccgg
     2221 tgtgctcatc aataatcaca atttcaccgt cttttacaat gtaatctacg ttgcgttcaa
     2281 ataatttatg ggcacgcaat gctgcactca catggtgtaa cagcgaaata cgagcagggt
     2341 gatataaact ttcgccctct tccattaacc ccatttgagt taagaaatcc tctactttca
     2401 ccataccacg ctcggttaaa tgagcctgtt tgtttttcag atctaaggta aaatcgccct
     2461 caccggtgta ctcttcactg tcttctttat cttgagcaat caaatgcgga atgatttgat
     2521 ctaccgcttg ataaatttgg gtcgcatctt ccgcaggccc tgaaataatt aacggcgtac
     2581 gagcttcatc aattaaaata gagtctactt catcaactaa tgcgtagtgt aactcacgtt
     2641 ggaaacgttc gtttttatcg tgagctaagt tatcacgcaa gtaatcaaaa cctaattcgc
     2701 tgttagtcga ataggtaata tctgcttggt atgcagcacg ttttgcatcg gaaggtaacc
     2761 ccggaatatt cactccaacg gttaagccta aaaactcaaa taacggacgg ttggtttccg
     2821 catcacggcg ggcgaggtaa tcattcaccg taacaacatg aacaccttta ccggtcaaag
     2881 cattcaaata acaagataaa gttgcagtta gggttttacc ttccccagta cgcatttctg
     2941 caatattacg ccccgttagc accataccgc cgatcagctg tacatcaaat ggtcgcatac
     3001 ctaatactcg tttgcttgct tctctcactg tcgcaaaagc ttcaggtaat aggctatcta
     3061 gcgatacacc atctgcaagg cgttgcttaa actccgctgt tttatccttt aacgcctcat
     3121 cagataattg ctcgaaggtt ggttcaagct tattaatttg tgccactcgc tttcttaaac
     3181 gttttaaagt gcgatcgttg cttgagccaa aaatagaagt cagtaatttt gaaatcattg
     3241 tgttattcct aaatataaaa atgggttaat caattaatgg taaaattaaa ttatatttag
     3301 gtttgtggcc cggctcgaat ggggtaagca ttttgtctat ccaagcggta ggagtttgca
     3361 aaaaattcgc aaaaatgcac cgcttgtggt ttaatttcca caattttatg gattaactct
     3421 gcaataaaaa gcgattgttg ctcaatatca ttagcggatt gagcatattg aagaaaagat
     3481 tgattaagaa cggtttcctg ttcactttca ttggcgtacg caacctgaat ttcaggtaaa
     3541 gcaagtatcg caaccattcc caaaaagagt tgcgaccaaa aaggcgttcg atgaaagtgt
     3601 tttaaaaata agctcattaa attaagataa atgaaaaaat tgggctaatt atacgctaaa
     3661 gtaaagccga atgagtaaat tattgggtca agtatcgtaa aatcaagcct aaaataagac
     3721 gaaatgtaaa gaaacagatg aaaaattcag cacctaaaaa gattaatgac attttacaga
     3781 actcaagctt aagccgtatt gtcgaaaaag caaacttcat taacgattta aaccaaaaaa
     3841 ttcagaaaag atggccagaa tcttatcgtg ggctttaccg agtagtgaat ttagttgata
     3901 atcagcttat tgtgagcgtt aagaatgcaa ccgtacggca aggtttaatc ttgcaacaaa
     3961 atatgttact aaaacagata caaaccgatt tacctgaaat aacccaactt gaatttagag
     4021 taaatccgag tgttacataa tttaattttg aagaggaaaa tagtatgaaa aaaatcgcac
     4081 tcattggttt taccaccgca atgctagggg cttgctcatt gctaccacaa aaaagcgtta
     4141 gcggtactta tgcaggtgaa ttaccgtgtg ctgactgtga gaaaatccaa gcccagctaa
     4201 ccttaaatgc agataaaacc taccaatatg atacggtata ttttaaagat aaaaaagaat
     4261 acacttaccg agacaaaggc acatacagtt gggaacccaa taagccaaat gtgattcgtc
     4321 tggaaaaagc atcaggcaat cttgccttcc aagtgtctga acaacacgtt gaactttgcg
     4381 acccaaatgg caacacggta aaaaacagca ataactataa actaggtaaa gtacgttaaa
     4441 agcagataaa aaggattctt aggaatcctt tttctttgta atctatcgcc gatcgcataa
     4501 aaaaaccccc aagcaatgtt tgctcgggga taacctaaaa atgaaaaagt attatctact
     4561 ttaacttaac caaggaaata tctatgaaaa cagtaaggaa tctttcagta tgaatggcgg
     4621 tgagaggggg attcgaaccc ccgatacagt ttcccatata cacgctttcc aggcgtgctc
     4681 cttcaaccac tcggacatct caccgaatca acaaaaagat ggcgtaaata tagcaatttt
     4741 acacggctct ttcaagtatt ttcttcacaa aataaaataa atcattttta tccacttatt
     4801 gcccaacacc taagcaattt agcgatttag gtacaagtcc gccagtcgct tcccattcac
     4861 cttgggtata taggtgcaaa gcaagtgcgt gaacgccatt agctaattcc tcagctaagc
     4921 aagcatagac agcacgatga cgtgcaactg ttctctgccc ttcaaattta tctgacacca
     4981 aagttaattt aaaatgcgat tctgctcctc tacctgagct gtgcatatag ctttcgtttt
     5041 cgacattaaa gaaaatcggc tgaaattctg tcttaatttt ttgaataatt gtctgttcta
     5101 ccgacatttt ttgctctcgt tcattgaaaa tcaaaacgaa ataatactat actaagcaga
     5161 aattttctcg aaatttattc aatatagaag gatttactat gaaacgctct aaaaaaacgt
     5221 tagtgttagc cactactttt gttgccgcat tactagccgg ctgccaaagc caatccaaca
     5281 cattaacatt ctctacccca acgccaactg ctgtatttaa cacaaacaat caaacggctg
     5341 cggtgaatgt aatgacacaa gatttacgca cctcgcgtga aatcgcaagc tacactaaaa
     5401 acggagaggt cattcgttta accgcagtgc ctgaagttgc tgcaatgttc caacaagccg
     5461 tccaacaaga cctgaatgca aaaggtttcc aaattgtaca aggtgcagcg aatgccaatg
     5521 tggtagtgaa tattcgtaaa ttctttgcca cagtagaaca aggaaactta cgctacaaag
     5581 ccgatgcaca agtgagtgta aaagtggcag tgcaaggttc taaaggaaac tttagcaaaa
     5641 acttcaacgc tacccgtggt tacgaaggtg catttggtgc aagcaatgat gaaatcaaga
     5701 aagtgttaga ccaagcctat tctgatgtgg tgaaagctat ttacaacgat aatgaaatcg
     5761 ccaatgcaat tcaccagttc aaataattaa ttttttctgg caagcggtca tctttcttcg
     5821 attttttgca aaaaacgtgg aaaaatgacc gcttgttgtt ttctcgtttt gtgatgtggc
     5881 tcgaaaaata agataaatta acttgattaa acgatcagta gcccccacaa tagagactca
     5941 tcgcagggag gaaaaatatg aataatcctt atcataatgt tagccaagaa tatcttgatt
     6001 atcttgctaa aaaagtaaag caaggaaagg aagatattaa aaacggtcgg atttattcac
     6061 ccgaagaggt cagaaaaaga gttattgctg ccgcttttag aggtgctgaa aatgccaaaa
     6121 aagttgcata atatttatta tactgaagag gccttaatag atttagagaa tattgccatc
     6181 tcagttagtg aatttaccgg ttatgcaagt agtggaatta g
//
LOCUS       DANLZC010000136         6173 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.136, whole genome shotgun sequence.
ACCESSION   DANLZC010000136 DANLZC010000000
VERSION     DANLZC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.136"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            10..1023
                     /gene="cas1c"
                     /locus_tag="PXE44_002132"
     CDS             10..1023
                     /gene="cas1c"
                     /locus_tag="PXE44_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HDL5589602.1"
                     /translation="MRKLQNTLYITTQGSYLHKERETLVVEQDRKKVAQLPIHSIGHI
                     FCFGNVLVSPFLMGFCGENNVNLAFFTENGRYLGRLQGRQSGNVLLRRAQYKKSETNP
                     VPVARNIIAAKIQASKRVLQRQIRNHGENADLQSTITALNYSLQQLKTADNLDLIRGI
                     EGDAAARYFGVFSHLIKENSGFHFDGRNRRPPRDGVNALLSFLYSIVGKDISGALQGV
                     GLDPQIGFLHADRSGRDSLAQDILEEFRAWWVDRMVLSLINRGQVKPDDFVTEASGAV
                     TLKPEARKLLFQTLQAKKQEKIIHPFLEEEVEIGLLPYIQAMLLARHLRGDLAEYPPF
                     LMR"
     gene            1090..1383
                     /gene="cas2"
                     /locus_tag="PXE44_002133"
     CDS             1090..1383
                     /gene="cas2"
                     /locus_tag="PXE44_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDL5589603.1"
                     /translation="MLMLITYDISFDDPEGQARLRRIAKHCLDYGVRAQYSVFECDVT
                     PAQWVTLKNKLLATYNPDCDSLRFYHLGSKWRNKVEHHGAKPSVDIFKDTLIL"
     repeat_region   1575..2610
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1575..1594
                     /rpt_unit_seq="cagccacctacgcgtggctg"
     gene            2860..4335
                     /gene="rng"
                     /locus_tag="PXE44_002134"
     CDS             2860..4335
                     /gene="rng"
                     /locus_tag="PXE44_002134"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDL5589604.1"
                     /translation="MSSTELLVNVTPSETRVALVENGILKELHIEREAKRGIVGNIYK
                     GRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTECVDENEKKQFLVKDISELVREGQ
                     DIVVQVVKDPIGTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKSLVE
                     PYCDELGGFIVRTAAEEATEESLKQDVEFLKRLWRKVLERKGKYSAKSMLYGELALAQ
                     RVLRDFIGTHINSVRIDSKLTYEQVREFLAEFMPELTDCVTLYTGQQTLFDAYGVEKA
                     IQKALDKRVDLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLAHTIFNTNIEAIQAIAH
                     QLQLRNLGGIIIIDFIDMQEEEHRERVLQSLQEALKGDRVKTNVNGFTQLGLVEMTRK
                     RTRESLERILCCDCQACQGRGTVKTVETVCYEIMREIVRVHHLFKTENIHVYAAKDVA
                     EYLINEESHALLAELQAFIGKKIEIKLEPYYHQEQFDVVVM"
     gene            4489..5991
                     /gene="lysS"
                     /locus_tag="PXE44_002135"
     CDS             4489..5991
                     /gene="lysS"
                     /locus_tag="PXE44_002135"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL5589605.1"
                     /translation="MSEVENQELDLNGEMLARREKLNKIREQGNAFPNTFRRDALAKD
                     LHAQYDAVEGETLKEQNIQVKVAGRIMLKRVMGKASFFTIQDVSGAIQLYVARDNLSD
                     SVYEEKVSLWDLGDIVGVEGALFKTKTGELTVRCSEVQLLTKSLRPLPDKHHGLTDLE
                     TRYRQRYLDLISNEESRRTFMIRSQVVSGIRKFFLERDFIEVETPMLQVIPGGAAAKP
                     FITHHNALDVDMYLRIAPELYLKRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEY
                     YQAYADYHDLMDNTEELLRQLALDILGTTDVPYGDYVFDFGKPFERITMHDAIVKYGN
                     GIQREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAE
                     ISPLARRNDENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEA
                     MFKDEDFIVALEHGLPPTAGEGLGIDRLVMIFANAPSIRDVILFPAMRQK"
BASE COUNT         1823 a         1129 c         1427 g         1794 t
ORIGIN      
        1 ttttcaacca tgcgcaaact acaaaacacc ctctacatta ccacccaagg tagctatttg
       61 cacaaagagc gagaaacctt ggtggtggag caagatcgga agaaagtggc acagttgccg
      121 attcattcca ttgggcatat tttttgtttt ggcaatgtgt tagtgtcgcc gtttttaatg
      181 ggattttgtg gcgaaaataa cgtaaattta gcgtttttta ccgaaaatgg gcgttatctt
      241 gggcggttgc aggggcggca aagtggtaat gtattgttac gccgagccca gtataaaaag
      301 tcggagacca accctgttcc tgtggctcgc aacatcattg cagccaaaat tcaagcatct
      361 aagcgggtgt tacaacggca aattcgtaat cacggcgaaa atgctgacct tcaatcgaca
      421 attaccgcat taaattacag cctgcaacag ttgaaaacag cagacaattt ggatttgatt
      481 cgtggcattg agggcgatgc tgcggctcgt tattttgggg tgttttctca tcttatcaaa
      541 gaaaatagcg gtttccactt tgacggacgt aatcgccgcc cacctcgtga cggtgtgaat
      601 gcgttgcttt catttcttta tagcattgta gggaaagata ttagcggggc attgcaaggc
      661 gtggggcttg atccccagat tggctttttg catgcagatc gttcggggcg agatagctta
      721 gctcaagata ttttggagga atttcgagcg tggtgggtgg acagaatggt gctgtcgctg
      781 atcaatcgag ggcaggttaa gcctgatgat tttgtgacgg aagccagtgg tgcggtaacg
      841 ctcaagcctg aagccagaaa attactattt caaaccttgc aagcgaaaaa acaagaaaaa
      901 atcatccacc catttttaga ggaagaagtg gaaatcggct tgttacctta tatccaagcg
      961 atgttattgg ctcgtcattt gcgtggcgat ttggcagaat atccgccgtt tttaatgcgt
     1021 taaaatctgc aaaattttgt tgaaatttga ccgcttgttt atgttttatt cattgaggaa
     1081 ttagcaacta tgctgatgtt gattacctac gacatttcat ttgatgaccc cgaagggcag
     1141 gctcgcctac gccgtattgc taagcattgt ttggattatg gtgtgcgggc gcaatattct
     1201 gtgtttgagt gtgatgttac tcctgctcag tgggtaacat tgaaaaataa attattggca
     1261 acttacaacc cagactgtga tagtttgcgc ttttaccatt taggcagtaa atggaggaat
     1321 aaagttgagc accatggcgc aaaaccttca gtggatattt ttaaagatac gctgatttta
     1381 taaatcgcta gcctgtagtt ctcataaaaa tgtagcaagg ttagcgaatc tatattgttc
     1441 tttaataatc aaggaattag aaaaaataag gcacaatata ttggcgatat gctatacttt
     1501 atccactctc tacaaaacgt tagcgaataa agggctaaaa agcctgatat ttcaatacgt
     1561 tatcttatag ggaccagcca cctacgcgtg gctgcgaatt gaaaccctat aacgagcaat
     1621 ggcattttga tctacggtaa accagccacc tacgcgtggc tgcgaattga aacttcttga
     1681 atacctccgg agtcagttaa gataaggcca gccacctacg cgtggctgcg aattgaaacc
     1741 accgctgcta cttcggttat cggagtagtt tgcacccagc cacctacgcg tggctgcgaa
     1801 ttgaaacgtg gcacagtaac acgagcagat gctgataaac gcccagccac ctacgcgtgg
     1861 ctgcgaattg aaaccagcag ccgttgttac cgtggtgcaa tgcttgcgcc agccacctac
     1921 gcgtggctgc gaattgaaac tacacttatt gccatagggt agtagtaatc ttttacccag
     1981 ccacctacgc gtggctgcga attgaaactg cgatttttgg atttttcggg cgataaatga
     2041 taccagccac ctacgcgtgg ctgtgaattg aaactcgtgg ctggttatac aaagaaaagc
     2101 attgttgggc agccacctac gcgtggctgt gaattgaaac ttaaatctaa agctaaagct
     2161 attttattaa ttaaagcagc cacctacgcg tggctgtgaa ttgaaacttt accgtttacc
     2221 gattggattt taatgctaga ggcagccacc tacgcgtggc tgtgaattga aaccctaaat
     2281 tgccgtaaaa atctgccatt agctacgcgc agccacctac gcgtggctgt gaattgaaac
     2341 ctcataacga atccaatcct gttcggttaa tccccgcagc cacctacgcg tggctgtgaa
     2401 ttgaaacagg ggcgtgtacc gcaatcaatt ttaattataa ccaagcagcc acctacgcgt
     2461 ggctgtgaat tgaaacttta tctgttgcgg tcaattgtat atcttgaatt tgcagccacc
     2521 tacgcgtggc tgtgaattga aacctcgtct tgaattggaa aatcaagggc gacaataggc
     2581 agccaccttc gcgtggctgt gtattgaaac ggtgtaccca caaatcctat gttttcgcca
     2641 ttggctgcta ccttcgcatc aatatatatt gaaatatcaa tggcaaattt gtatgttgtt
     2701 aattagactg attgcatctg ctatgtatca tagtaatgac gaaaatattc tattttgcaa
     2761 aaaataatca gaaattaccc gcttgttcga actgagtttt tgttttctaa gatgtgaaat
     2821 gatacacttt agatagtttt aaaatataga gacagtaaga tgagtagtac ggaattatta
     2881 gtgaatgtta ccccaagcga aacaagggta gcgttggtag aaaacggaat attgaaagaa
     2941 ttacatattg agcgggaagc taaacgcggg attgttggta atatttataa gggaagggtg
     3001 acaagggtat taccaggtat gcaatctgct tttgtggata tcggcttgga gaaggctgct
     3061 tttttgcacg cttctgacat cgtttcgcat acggaatgtg ttgatgaaaa tgaaaagaag
     3121 caatttctgg ttaaagatat ttctgaattg gtgcgtgaag ggcaagatat agttgtacaa
     3181 gtagtgaaag atccaattgg tactaaagga gctaggctca ctactgatat tacgttacca
     3241 tctcgttatt tagtgtttat gccagaaaat agccatgtgg gagtatctca gcggattgaa
     3301 agtgaagaag agcgtgctcg tttaaaaagc ttggttgagc cttattgtga tgaacttggg
     3361 ggctttattg tacgtaccgc tgcagaagag gcaactgaag aaagtttaaa gcaagatgtt
     3421 gaatttttaa aacggttgtg gcgtaaagta ttagaacgaa aagggaaata ttccgcaaaa
     3481 tcaatgcttt atggagagct tgcgcttgct caaagggtac tgcgtgattt tattggtaca
     3541 catatcaatt cggttagaat cgattcaaag ctaacttatg agcaagtgcg agaattttta
     3601 gctgagttta tgccagaatt aacggattgt gtcaccctct ataccggtca gcaaacttta
     3661 tttgatgcct atggtgtaga aaaggcaatc caaaaggcat tggataaacg ggttgattta
     3721 aaatcggggg gctatttaat tattgagcaa actgaggcga tgacgactat tgatattaat
     3781 actggtgctt ttgtcggaca tcgtaacctt gcacatacga ttttcaacac aaatattgaa
     3841 gcaatacagg caattgcaca ccagctacag ttgcgtaatt taggtggtat tatcattatt
     3901 gattttattg atatgcaaga ggaagaacat cgtgagcgcg ttttacaatc gttacaagag
     3961 gcattaaaag gcgatcgtgt taaaaccaat gtgaatggct ttactcagct tggcttggtg
     4021 gagatgacca gaaaacgtac aagggagagt ctagagcgaa ttctttgttg cgattgccaa
     4081 gcttgccaag ggcgtggcac agtgaaaact gtagagacgg tttgctatga aattatgcgt
     4141 gaaattgtgc gtgtacatca tttgtttaag acagaaaata ttcacgttta tgcagctaaa
     4201 gatgttgctg aatatttaat taacgaggaa agccacgcat tattggctga gcttcaggcg
     4261 tttatcggta aaaaaattga gattaaactt gagccatatt atcatcaaga gcagtttgat
     4321 gtggtcgtga tgtaatctat tacttggttg aggctaaata gttagcctca atatcgttag
     4381 ttacaaaaaa tttagaaaat ctgaccgctt gtttcctatc caaattcctc aaaataacgt
     4441 ataatcgtcc agttttattt atcctttttt gaaatctaag gtaattcaat gtctgaagta
     4501 gaaaatcaag aattagatct aaacggtgaa atgttagccc gccgtgaaaa attaaacaaa
     4561 atccgtgagc agggtaacgc atttccaaat acattccgtc gtgatgcgtt agccaaagat
     4621 ctgcacgctc aatatgatgc agtggaaggt gaaaccttaa aagaacagaa tattcaagtg
     4681 aaagttgccg gtcgtattat gttaaaacga gtaatgggta aggcatcttt ctttaccatt
     4741 caagatgtga gcggtgcgat tcagctttat gtagctcgtg ataacttaag tgacagcgtt
     4801 tacgaagaaa aagtcagcct ttgggattta ggcgacattg tgggcgtcga aggtgcgtta
     4861 tttaaaacca aaaccggtga attaacggtt cgttgctctg aagttcaact tttaaccaag
     4921 tctttacgcc cgttgccgga taaacatcac ggtttaacgg atttagaaac tcgttatcgt
     4981 cagcgttatt tagacttgat ttctaatgaa gaatctcgcc gtacttttat gatccgctcg
     5041 caagtagttt caggcattcg caaattcttc ttagaaagag actttattga agttgaaacg
     5101 ccaatgttgc aagtgatccc gggcggtgcg gcagcgaaac cgtttatcac tcatcataat
     5161 gcgttagatg tggatatgta tctgcgtatt gctccagagc tttacttaaa acgtttagtt
     5221 gtgggtggtt ttgagcgagt atttgagctt aaccgtaact tccgtaacga aggggtttca
     5281 gtacgccaca atcctgaatt tacaatgatt gagtactacc aagcctatgc ggattatcac
     5341 gatttaatgg ataataccga agaactgtta cgccaattag ccttagatat tcttggcaca
     5401 actgacgtac cttatggtga ttatgtattc gatttcggta aaccgtttga gcgtattacg
     5461 atgcacgatg cgattgtgaa atatggtaac ggcattcaac gtgaagattt agacagcttc
     5521 gagaaaagtg tggaaattgc gaaaggctta ggcattgaaa ttcaaaaatc ttggggttta
     5581 ggctcggtag tcaatgcgat ttttgaagaa gtggcagaac atcaattaat tcagccaact
     5641 ttcttgatgg ctcacccggc agaaatttca ccattagctc gccgtaatga tgaaaaccca
     5701 gaggtaactg atcgttttga gttatttatc ggtggtcgtg aaattggtaa tggtttctca
     5761 gaattaaacg atgcagaaga ccaagcagag cgttttgatg ctcaagttgc ggcgaaagat
     5821 gccggtgatg atgaagcaat gtttaaagat gaagacttta tcgtggcatt ggaacacggt
     5881 ctgccgccaa cagcgggtga aggtttagga attgaccgct tagtaatgat tttcgctaac
     5941 gccccatcaa tccgtgatgt aattttattc ccagcgatgc gtcagaagta atcagaaata
     6001 gaacaaaagc acacttcggt gtgctttttt gtattacaag cggtcaaatt tataataaat
     6061 atttcaaact taagtgagca taaggcttat tataaataaa acgttccgaa aaaacattga
     6121 atttcattaa aaaattcctt tatttatcaa ataaatgcga tttgtttcgg aac
//
LOCUS       DANLZC010000137         6138 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.137, whole genome shotgun sequence.
ACCESSION   DANLZC010000137 DANLZC010000000
VERSION     DANLZC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.137"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            24..1940
                     /gene="iolC"
                     /locus_tag="PXE44_002136"
     CDS             24..1940
                     /gene="iolC"
                     /locus_tag="PXE44_002136"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDL5589606.1"
                     /translation="MATVNHQDRPLDLICLGRVAVDFYGQQIGSRLEEMTTFSKYLGG
                     SSGNVAYGTAVQGLRSSMLARVGDEHMGRFLREELQSVGVDTSHLITDKERLTALVIL
                     GIKDKETFPLIFYRDNCADMAITKDDFSEAYIASSRCLAITGTHLSNPKTREAVLTAL
                     EYAGRNEVKRALDIDYRPVLWGLTSLGDGETRYIDSAEVTKSLQEVLHHFDLIVGTEE
                     EFHIAGGSTDTLTALKNVRKVSNAVLVCKRGALGCSVFEGELPNDLDGGLNVYGVRVE
                     VLNVLGAGDAFMSGLLRGYLNNEGWEQACHYANACGALVVSRHGCSPAMPTKRELDDY
                     LTRAESVPRPDLDERLNHLHRVTTRKKQWENLCIFAFDHRKQLVDMANKVGADLARIP
                     KLKQLLLKAMEQTATQSGLPQNQAGILADTTFGQDVLNEITGKDYWIARPIEEPSSRP
                     LELEYGDLGSQLASFPRDHVIKCLAFYHPNDESGLKARQDRKLKEVYQACCRTGHELL
                     LEIILPADMPQSEAFYNQAIAHFYQLGIKPEWWKLPDVSRAQWQAISATIEANDPDCR
                     GILILGLDAPLEVFKDTFEQAKGCEKVKGFAVGRTIFGEPSEKWLAGELDDNTLISAV
                     SEKYRTLIDLWQQR"
     gene            2230..3570
                     /locus_tag="PXE44_002137"
     CDS             2230..3570
                     /locus_tag="PXE44_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HDL5589607.1"
                     /translation="MSCNTSPKRFHASWESIKQHQVPEWYEDAKFGIFIHWGIYSVPA
                     FAPPTCQLGEIDIDEQWFCNNPYAEWYFNSINVKKGPTYEHHIKTYGESFAYNDFLPL
                     WKAEKWQPAEWAKLFAKAGAKYVVLTTKHHDGFCLYPSKYTDFNCMQAGPKRDLMGEL
                     TENVREQGLKMGAYYSGIIDWTYSSAPIFTESQNFSNACPTYEYADYAYKQVVELIDR
                     YKPDVLWNDIGWPKVGEHMLPHLFAHYYNSVQEGVVDDRWNKLWCDFTSKEYKHGVAS
                     RDKKWEMCRGMGLSFGYNQVEDESHLISVKDLISLLVETVADNGNLLLNIGPKADGTI
                     PQEQVERLLALGSWLEINGEGIYKSRCSQHQTEQIGDVTYRYTKVGADLYLYIDGLTE
                     AQISLPLSVYGELQALNTELKFTIENTAQGRVLHIQNYQPDWYVLGFKIADGEK"
     gene            3573..4913
                     /locus_tag="PXE44_002138"
     CDS             3573..4913
                     /locus_tag="PXE44_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL5589608.1"
                     /translation="MSVSQRFIDFMNAHVAPVARRMENQPHISAIRDGFIVVLPFLIV
                     GSFIMILLIPPFDENTQNAFGQAWWRFANWASPYGWNFFQMSFNAISLFTSASIAYNL
                     AKAYKREPLPAAFLSVMAFLLVAAPVKDGMMDIKFFGGIGLFSAIFIAIYSVEMTRLL
                     EFLKIKIRLPKEVPHAVAESLNIVIPILAILLTLYPFAIWVESATGRNIPQLIMDFMA
                     PLIAVSDSLGAICLFVIATHLLWFLGINGSLVLMQLWTPFLLQNMAANLAAFQAGEPL
                     PFIITNSFWDFYIVHGASGGVIALAFLLVRSKSAHLRSIGKIGLVPSFFSIGEPIVYG
                     VPMVVNPLFFIPLIFAPLANAVIAYLILDFNLIHRVYLMAPWTTPAPIGAYLVSAGDI
                     WAPILSIALIILDIVIYYPFFKMYEKVCVEKEKAQNLDEKAQQELLEKARMAAQ"
     gene            5051..5875
                     /locus_tag="PXE44_002139"
     CDS             5051..5875
                     /locus_tag="PXE44_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL5589609.1"
                     /translation="MNGPTQLAHFQEQIRKEYDGLSKRLKQVAQYVLDNGNSVVFDTV
                     STIAERADVPPSTLIRFANAFGFSGFNEIKQLYRQDMMEGTSRYTERVQLFRQIEPLD
                     EQNSELNHILDVFVQGNTQALQQLSTQVSEEQLAQTVDILHNAKRIFIVGLKRSFSIA
                     NYLNYALHHLDYDVFMIDGSGGMFDEQLSRIREGDAVIAVSFSPYANETLNVVTATAR
                     PGVKHIAITDSQLSPLLSFSDVSFIIKEAQVSGFRSQCATMTLAQTIAVSLALKKA"
     gene            complement(5919..>6138)
                     /locus_tag="PXE44_002140"
     CDS             complement(5919..>6138)
                     /locus_tag="PXE44_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL5589610.1"
                     /translation="EIINALGNKENIKQVEACLTRLRVVLHNNGLLNKSALKKLGAVD
                     VIKVGDTQQIIFGAKAGQYRDEINALIG"
BASE COUNT         1731 a         1313 c         1381 g         1713 t
ORIGIN      
        1 tttacttgtt gtaaggagcg gttatggcaa cagttaatca tcaagatcgt ccattggatc
       61 ttatctgttt agggcgtgtg gcggtggact tttatgggca acaaatcggt tcccgtttgg
      121 aagagatgac cacattttca aaatacctcg gcggctcgtc aggcaatgtg gcttacggta
      181 cggcggtgca aggtttacgt tcttcaatgc tggcaagggt cggtgacgaa catatggggc
      241 gttttctacg tgaagagcta caaagcgtag gcgtggacac cagccattta attaccgata
      301 aagaacgctt aactgcactg gttattctcg gcattaaaga taaagagact tttccgctga
      361 ttttctatcg tgataactgt gcggatatgg caatcaccaa agacgatttt agcgaagcct
      421 atattgcttc cagccgttgc ttagcaatta ccggcacgca cctgtcaaac cctaaaaccc
      481 gtgaagctgt gctgaccgca ctcgaatatg cgggtagaaa tgaggtaaaa cgagcgttag
      541 atattgatta tcgccctgtg ttatggggat taacctcact cggtgatggc gaaacccgtt
      601 atatagacag tgccgaagtc accaaatcgc tacaagaagt attgcaccat ttcgatctga
      661 ttgtcggcac agaggaagaa tttcatatcg caggcggttc gaccgatact ctcactgcac
      721 tcaaaaatgt gcgtaaagtc tctaatgccg tattagtgtg caaacgtggg gcattagggt
      781 gttcagtatt tgaaggcgag ctgccgaatg atttagacgg tggcttgaat gtgtacggcg
      841 tgcgtgtaga agtgctgaat gtgctgggtg caggcgatgc ttttatgtcg ggcttactgc
      901 gtggctactt aaataacgaa ggttgggaac aagcgtgcca ctatgccaat gcgtgcggag
      961 cgttagtggt atctcgtcac ggctgttcac cggcaatgcc gaccaaacgt gaacttgacg
     1021 attatttaac ccgtgcagag agtgtaccac gtccggattt agatgagcgt ttaaaccact
     1081 tacaccgtgt caccacccgc aaaaaacagt gggaaaatct ctgtattttt gcttttgatc
     1141 accgcaaaca gttggtggat atggcaaata aagtcggtgc agatcttgct cgcataccga
     1201 aactgaaaca gctgctatta aaagcgatgg aacaaaccgc aactcaatcc ggcttaccac
     1261 aaaatcaagc agggatccta gccgatacca ctttcgggca agatgtactt aatgagatta
     1321 ccggcaaaga ttactggatt gcccgcccga ttgaagagcc gtcttcccgc ccgctggaat
     1381 tagagtatgg agatttaggc tctcagctag catcattccc acgcgatcac gtcatcaaat
     1441 gcttggcatt ttaccacccg aatgatgaaa gcggtttaaa agcccgccaa gatcgcaagt
     1501 taaaagaggt gtatcaagcc tgttgccgta ccggtcacga gctattgttg gaaattattc
     1561 tgcctgctga tatgccacag agtgaggctt tctataacca agcgatagca catttctacc
     1621 aacttggcat taagccggaa tggtggaaat tgccggatgt ttctcgcgca caatggcaag
     1681 cgatttccgc caccattgag gcaaacgatc cggactgtcg tggtatttta attttaggtt
     1741 tagatgcccc gttagaggtg tttaaagaca cctttgaaca ggcaaaaggc tgtgagaaag
     1801 tgaaaggctt tgcagtcggc cgcaccattt tcggtgaacc gtccgaaaaa tggcttgccg
     1861 gtgaattgga cgacaatacc ttgatttctg cggtatcgga gaaatatcgc actttaatcg
     1921 atctctggca acaacgttaa tcatattgct ttaagtgaaa accccttagc ttatgatatt
     1981 aagcaagggg ttttgttttg caaaaaatcc ggctaaacag accgcttgct agccgtaaaa
     2041 atgcccgaat tttcctaaaa aagcgaatat cgggcattcc tcaatctgct tatcacaaaa
     2101 taagtgtgat gaagttcaca tttcatacat aaaccccccc cctttttatc gaaacaaatt
     2161 gttttatttt tatcaaaatg gaaaatgtat ttcagtttag ttctatttag attcaatagg
     2221 agtctgtgta tgagttgcaa cacttcacca aaacgtttcc acgccagttg ggagtcgatc
     2281 aaacagcacc aagtaccaga gtggtatgaa gatgctaagt ttgggatttt tattcactgg
     2341 gggatttact ccgtacccgc ctttgcccca ccgacctgtc agttaggtga aattgatatt
     2401 gatgaacagt ggttctgtaa taacccttat gcagaatggt attttaattc catcaatgtc
     2461 aaaaaaggcc cgacctacga acatcatatc aaaacttatg gcgaatcctt tgcctataac
     2521 gacttcctgc cactatggaa agcggaaaaa tggcaaccag cagaatgggc gaaactgttt
     2581 gccaaagctg gcgccaaata tgtggtatta accaccaaac atcacgatgg tttctgtctc
     2641 tatccaagta aatataccga tttcaactgt atgcaagccg gaccgaagcg ggatttaatg
     2701 ggcgagctga ctgaaaatgt gcgggagcaa ggcttaaaaa tgggcgctta ctactcgggc
     2761 attatcgatt ggacttattc ctctgccccg attttcaccg aaagtcaaaa cttctcaaac
     2821 gcttgcccga cttacgaata cgctgactac gcctataaac aagtggtaga gctgatcgac
     2881 cgctataaac cagatgtatt gtggaatgat attggctggc caaaagtggg cgagcatatg
     2941 ttaccacacc tatttgctca ttattacaac agcgttcaag agggcgtagt ggacgaccgc
     3001 tggaacaaac tatggtgcga tttcacctcc aaagaataca aacacggcgt ggcttcccgt
     3061 gataagaaat gggaaatgtg ccgtggtatg ggcttatcgt tcggctataa ccaggtagaa
     3121 gacgaaagcc atttgatttc ggtgaaagac ttgatttccc tattggtaga aaccgtagcc
     3181 gacaacggca atttattgct gaatatcggc ccgaaagcag atggcactat tccgcaagag
     3241 caagttgaac gcttattagc actggggagc tggctggaaa taaatggaga gggaatctat
     3301 aaatcccgtt gtagccaaca ccaaaccgag caaatcggtg atgtaaccta tcgttatacc
     3361 aaagttgggg cggatctcta tctttacatt gatggcttaa ccgaagccca aatcagcttg
     3421 ccactgtcag tttatggtga gctacaagca ctcaataccg agctgaaatt taccattgaa
     3481 aacaccgcac aaggcagagt gttacatatt caaaactacc agccggattg gtatgtatta
     3541 ggctttaaaa ttgcagatgg ggagaaatag gtatgtccgt ttcacaacgt tttattgact
     3601 ttatgaatgc ccacgttgct ccagtagcac gtcgaatgga aaatcaaccg catatttccg
     3661 ctattcgaga cggttttatt gtagtgttac cattcttaat tgtcggcagc tttattatga
     3721 ttttgctaat tccgccattt gatgaaaata cccaaaatgc tttcgggcaa gcttggtggc
     3781 gttttgcgaa ttgggcaagc ccttacggtt ggaatttctt ccaaatgtcg tttaatgcga
     3841 tttcattatt tacctctgcc agtatcgcct acaaccttgc caaagcctat aaaagagagc
     3901 ccctgccagc ggcatttctc tcggtaatgg catttttgtt agtggctgca ccggtcaaag
     3961 acggaatgat ggatattaaa ttctttggcg gtattgggct attctctgcg atttttattg
     4021 ccatttattc ggtagaaatg acccgcttgt tggagttctt aaaaattaaa atccgcttac
     4081 cgaaagaagt gccgcacgct gtagcagaat ctctgaatat cgtgattcca attttagcga
     4141 ttttactgac tctctatccg tttgccattt gggtggaaag tgccacaggt cgtaatattc
     4201 cacaactaat tatggacttt atggcgccat tgattgctgt gagtgactcg ctaggggcaa
     4261 tttgcttgtt cgttattgct acccatttgc tctggttctt aggcattaac ggctcattgg
     4321 tactaatgca actttggaca ccattcctac tgcaaaatat ggcggcaaac ttagcagcat
     4381 tccaagctgg tgaaccgctc ccctttatca tcaccaattc cttctgggat ttctatatcg
     4441 tacacggggc aagtggtggt gtaatagcct tggcattcct gctggtacgc agtaaatcgg
     4501 cacacttacg ctctatcggt aaaatcggct tggttccgtc cttcttctct atcggtgaac
     4561 caattgtgta tggtgtgcca atggtggtta atccgctgtt ctttatcccg ctgattttcg
     4621 caccactagc gaatgcggtt attgcctacc tgattttaga ttttaacctt attcaccgtg
     4681 tttacttgat ggcaccgtgg acaacaccgg caccgattgg ggcatatctg gtttctgccg
     4741 gtgatatttg ggcaccaatt ttaagtattg cactgattat tttggatatt gtgatctact
     4801 atccattctt caaaatgtat gaaaaagtct gcgtcgagaa agaaaaagca caaaatctag
     4861 atgaaaaagc acaacaggag ttgctagaaa aagcacgaat ggcagcacaa taacacttat
     4921 ttctagcgta attgagaggg gctaatcgaa agattagccc ttatttttgc aaaaaattga
     4981 caaaatttat ccgcttgtaa acaagaaaag cgtataatga aataaaaaat tcaagcagag
     5041 ataatttgaa atgaatggac ctactcaatt agcacatttt caagaacaaa tccgaaaaga
     5101 gtatgacgga ctaagtaaac gtttaaaaca agtggcacag tatgtgctgg ataatggcaa
     5161 cagcgtggtg tttgatacgg tttctaccat tgctgaacga gctgatgttc ccccttcaac
     5221 cttaatccgt tttgcgaatg cgtttggatt tagcggtttt aatgaaatta aacagcttta
     5281 tcgccaagat atgatggaag gcacatcacg ttacaccgaa cgtgtgcaac tttttcgtca
     5341 aattgaaccg cttgatgagc aaaacagtga gctcaatcat attttagatg tgttcgtaca
     5401 gggtaatacg caggcactgc aacagttatc gacacaagtc agcgaagagc agttagcaca
     5461 aacggtagat attttacaca atgccaaacg catttttatt gtgggtttaa aacgctcatt
     5521 cagcattgcg aactacctaa actatgcgtt gcaccatttg gactatgatg tctttatgat
     5581 cgatggttcg ggcggtatgt ttgacgagca attaagccga atccgtgaag gtgatgcggt
     5641 gattgccgtg agcttttccc cgtatgcgaa tgaaacactc aacgtggtga cagcaactgc
     5701 cagaccgggg gtaaaacata ttgcgatcac tgatagccaa cttagcccac tcctttcatt
     5761 tagcgatgtg tcgtttatta ttaaagaagc acaggtgagt ggttttcgat cccagtgtgc
     5821 cacaatgacg ctcgcacaaa cgattgccgt ttcactggcg ttgaaaaaag cctagattaa
     5881 gaaagtgaaa gacctgcgta aatacaggtc tttgtctatt aaccgattaa agcgttaatt
     5941 tcatcacgat actgcccggc ttttgcacca aaaataattt gttgcgtatc accgacttta
     6001 atgacatcaa ccgccccaag ctttttcagt gccgacttat ttaataaacc gttgttatgt
     6061 aacaccacac gtaaacgggt taaacaagct tcaacttgct tgatattctc tttgtttcct
     6121 aaagcattaa taatttct
//
LOCUS       DANLZC010000138         6106 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.138, whole genome shotgun sequence.
ACCESSION   DANLZC010000138 DANLZC010000000
VERSION     DANLZC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.138"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(201..1487)
                     /gene="purD"
                     /locus_tag="PXE44_002141"
     CDS             complement(201..1487)
                     /gene="purD"
                     /locus_tag="PXE44_002141"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL5589611.1"
                     /translation="MNILIIGNGGREHALAWKVRQSPLADKVFVAPGNAGTAMENGIE
                     NVAISATDVPALVTFAKQNSVGLTIVGPEAPLVVGVVDAFRAHGLTIFGPTQAAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMT
                     LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRV
                     GEGDSGLNTGGMGAYSPAPVVTKAIHQRVMDEIIYPTVQGMASENTPYTGFLYAGLMI
                     MPNGQPKVIEFNCRFGDPETQPIMMRLESDLVELCLKACEGKLDSVQSKWCEQAALGI
                     VLAAAGYPGDYRKGDEIIGIPTSTSNQKVFLAGVEQKEGKLLTNGGRVLCATALGNSV
                     FEAQQQALQLAEQIQWQGKFYRRDIGYRAVARKQAK"
     gene            1721..2095
                     /gene="rpsF"
                     /locus_tag="PXE44_002142"
     CDS             1721..2095
                     /gene="rpsF"
                     /locus_tag="PXE44_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL5589612.1"
                     /translation="MRHYEIVFMVHPDQSEQVPAMIERYTASVKEAGGQVHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQSVIDELETNFRYNDAVLRNLIVHTKAAVTEASPM
                     VKAKESKVAEAVAEVESEEAGE"
     gene            2070..2444
                     /gene="priB"
                     /locus_tag="PXE44_002143"
     CDS             2070..2444
                     /gene="priB"
                     /locus_tag="PXE44_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDL5589613.1"
                     /translation="MNQRKQASNLPIDNCLILSGTVSSLVKQSLNPLGVPHYSFWLEH
                     RSIQTEVNLERQAWCKIQVVLAGNQFSYLTQQIKLGESVRIKGFIHTHKDFNGLNQLV
                     LHTEQIEFIDQEKPNGTLFPSS"
     gene            2419..2646
                     /gene="rpsR"
                     /locus_tag="PXE44_002144"
     CDS             2419..2646
                     /gene="rpsR"
                     /locus_tag="PXE44_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL5589614.1"
                     /translation="MARYFRRRKFCRFTAENVVEIDYKDIATLKNYISESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLALLPYTDNHQ"
     gene            2662..3111
                     /gene="rplI"
                     /locus_tag="PXE44_002145"
     CDS             2662..3111
                     /gene="rplI"
                     /locus_tag="PXE44_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL5589615.1"
                     /translation="MQVILLDKVAHLGSVGDQVTVKSGFARNFLIPQGKAVMATAANI
                     AHFEARRAELEAKAAAALATAQERAAKIAALAAVSVSATAGDDGRLFGSVSAKDIAEA
                     LTAAGVGVAKSEVRLGEGPLRTTGEHQVKIHLHPEVNATVAVNVVAE"
     gene            complement(3175..3951)
                     /gene="dnaQ"
                     /locus_tag="PXE44_002146"
     CDS             complement(3175..3951)
                     /gene="dnaQ"
                     /locus_tag="PXE44_002146"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDL5589616.1"
                     /translation="MTEEKIIRQVVLDTETTGMNMAGGPPQIGHNIIEIGAVEVINRR
                     LTGRTYHVYIKPPREVDEEAIAVHGITNEFLQDKPVFAEIAEEFLDFIKGAELIIHNA
                     PFDVAFMDQEFSYLPNPPAKTAEMCTVTDSLQMARRMYPGKRNNLDALCDRLGIDNSK
                     RVLHGALLDAEILADVFLMMTGGQISLLGEEETEVVSEVIDIEEEFSAIVLNADEAII
                     LKANDEEEESHLALLKLIEKKSKGNCLWTKALTDTAEISH"
     gene            complement(4039..5709)
                     /gene="ettA"
                     /locus_tag="PXE44_002147"
     CDS             complement(4039..5709)
                     /gene="ettA"
                     /locus_tag="PXE44_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDL5589617.1"
                     /translation="MSTQFVYTMHRVGKVVPPKRHILKDISLSFFPGAKIGVLGLNGA
                     GKSTLLRIMAGVDKEFEGEARPQPGIKIGYLPQEPKLAPQQTVREAVEEAVAEVKNAL
                     TQLDEVYAMYADENADFDKLAAKQAELEAIIQAHDGHNLQNQLERAADALRLPDWDAK
                     IEHLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDYEGTVVAITH
                     DRYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKEKRLAQEQAAESARQKSIEKELEW
                     VRQNPKGRQAKSKARMARFEELNSGEYQKRNETNELFIPPGPRLGDKVIEVQNLTKSY
                     GDRTLIDDLSFAIPKGAIVGIIGANGAGKSTLFRMLSGKEQPDSGSIVLGETVVMASV
                     DQFRDAMDDKKTVWEEVSNGQDILTIGNFEIPSRAYVGRFNFKGVDQQKRVGELSGGE
                     RGRLHLAKLLQRGGNVLLLDEPTNDLDVETLRALENAILEFPGCAMVISHDRWFLDRI
                     ATHILDYGDEGKVTFYEGNFSDYEEWKKKTFGEAATQPHRVKYKRIAK"
BASE COUNT         1613 a         1399 c         1292 g         1802 t
ORIGIN      
        1 ctatttaaag atctttttag tctttgccaa tattgacagt tttttccaca tatagggcac
       61 gcagagtcat ttagaaaaga gtttactgca tcgtttaatg atagtgattt aatttcgggg
      121 cctgcatcga gttcagtgat attaatttca ttaagtgttg tactcataat aaatttttcc
      181 ttgttttaag agtttataaa ttatttcgct tgttttcttg ccactgctcg gtagccaata
      241 tctcggcgat agaatttgcc ttgccattga atttgttcgg caagttgtaa ggcttgttgt
      301 tgagcttcaa atacactgtt gccaagtgcg gtggcacaaa gtacacgtcc accgttggtg
      361 agcagttttc cttctttttg ctcaacccct gctaagaaaa ctttttgatt ggaggtagag
      421 gtcggaatgc ctatgatttc atcgccttta cggtaatcgc ccgggtagcc tgcggcagct
      481 aaaacaatgc ctaatgcggc ttgttcacac cattttgatt gcacgctatc caatttgcct
      541 tcgcacgctt ttaagcacaa ttcaacgagg tctgattcca aacgcatcat aatcggctga
      601 gtttcagggt ctccaaaacg gcagttaaat tcaatcactt tcggctgacc gtttggcata
      661 atcattaagc cggcatagag aaaaccggtg taaggggtat tttcgcttgc cattccttgc
      721 acggtcgggt aaataatttc gtccattaca cgttggtgga ttgcctttgt taccaccgga
      781 gcaggggagt aagcccccat tccgccggta tttaagccgc tatcgccctc gccaacacgt
      841 ttgtggtctt gtgaggttgc cattggttcg acatttttgc cgtccaccat cacaataaaa
      901 ctggcctctt ctccgtctaa aaattcctcg atcaccacac ggctgccggc ttcaccaaag
      961 gcgttgcctg acagcatatc acgcactgca tcttccgcct cttgtaaagt cattgcaaca
     1021 attacgcctt tgcctgcggc taaaccgtct gctttgatta caatcggtgc acctttttgt
     1081 tttaagtagg caagtgccgg ttcaatttcg gtgaaatttt ggtattctgc ggttggaatc
     1141 tgatggcgag ccaagaaatc tttggtaaat gctttagaac cttctaattg agcagcggct
     1201 tgggtcgggc cgaaaatggt taaaccgtgg gctcgaaaag catcaaccac gccaactact
     1261 aacggtgctt cagggccgac aatcgtcaaa cctacgctat tttgtttagc gaatgtaact
     1321 aaggcaggca cgtcagtggc ggaaatagct acattttcaa tgccgttttc catcgctgtg
     1381 ccggcattgc cgggggcgac aaagactttg tctgcgagcg gcgattgacg aactttccaa
     1441 gcaagagcgt gttcacgccc accgttgccg ataattaaaa tattcattgg gattccttgc
     1501 aaaaatgata aagcaaacgt ttgcgtattg tacttgattt gtttatctgc tgacaagcag
     1561 tcgtttttct cggatttttt gcaaaatacg aaattgttgc ttgcaaaata ggcttttttc
     1621 agtataatcc tgccgttcag tcacattcgt tttttaatcc ttgcttccac aagttggtga
     1681 ctggctaaag ttggaggctg ctaacccgta aggatagtca atgcgtcact acgaaatcgt
     1741 ttttatggtt cacccggacc aaagcgaaca agtaccagca atgattgagc gctacacagc
     1801 gtctgtaaaa gaagctggcg gtcaagttca tcgcttagaa gattggggtc gtcgtcaatt
     1861 agcataccca atcaacaaac ttcacaaagc acactatgtg ttaatgaatg tagaagcacc
     1921 tcaaagtgta atcgacgagt tagaaactaa cttccgttat aacgatgcag ttcttcgtaa
     1981 cttaatcgtt cacactaaag cggcagtaac tgaagcgtca ccaatggtga aagcgaaaga
     2041 aagcaaagtt gctgaagcgg tagctgaagt tgaatcagag gaagcaggcg agtaatttgc
     2101 cgattgataa ttgtttaatc ttaagcggaa cggtttccag cttggttaaa caaagcctaa
     2161 acccccttgg tgtgcctcat tacagttttt ggttagaaca tcgttcaatc caaaccgaag
     2221 tgaatttaga acgccaagcg tggtgcaaaa tccaagtggt tttggcaggc aatcaattta
     2281 gttatttaac ccaacaaatt aagctcggtg agagcgtgag aattaaaggt tttatccata
     2341 ctcacaaaga ctttaacggt ttaaatcagt tagtcttaca caccgaacaa attgaattta
     2401 tagatcagga gaagccaaat ggcacgttat ttccgtcgtc gtaagttctg ccgtttcaca
     2461 gcggaaaatg ttgttgaaat cgattacaaa gatatcgcta cattaaagaa ctacatttct
     2521 gaaagcggca agattgttcc tagccgtatt actggtactc gtgcgaagta tcaacgtcaa
     2581 ttagctcgtg caattaaacg cgctcgctac cttgcgttac ttccatatac tgataaccac
     2641 caataatcag gagagagtac aatgcaagtt attttattag acaaagttgc tcacttaggt
     2701 tctgtgggcg accaagtaac cgttaaatct ggttttgctc gtaacttctt aatcccacaa
     2761 ggtaaagcgg ttatggcaac tgcggcaaac attgctcact ttgaagcacg tcgtgcagaa
     2821 ttagaagcaa aagctgcagc agcattagca actgcacaag aacgtgcagc gaaaattgca
     2881 gcattagcag cagtatcagt atctgcaact gcaggtgatg atggtcgttt attcggttct
     2941 gtatcagcta aagacatcgc tgaagcatta actgcagcag gtgttggagt tgctaaatct
     3001 gaagttcgtt taggtgaagg tccacttcgt acaaccggcg aacaccaagt taaaattcac
     3061 ttacaccctg aagtgaatgc aactgtagca gtaaacgttg ttgctgaata atcagtagaa
     3121 atagaaaacc cagcgaaagc tgggtttttt gtttgcttta tttattggtt agaatcagtg
     3181 agatatttct gctgtgtcgg ttaatgcttt tgtccatagg caatttcctt tggatttttt
     3241 ctcaatcagt ttcagaagtg ctaaatgcga ctcttcctct tcatcgtttg ctttaagaat
     3301 aattgcctca tcggcattta acacaattgc agaaaattct tcttcaatat caataacttc
     3361 acttaccact tcagtttctt cttcgcctaa taatgaaatc tgaccgccgg tcatcattag
     3421 gaatacatca gctaagatct ccgcatccag taacgccccg tgtagtacac gcttgctatt
     3481 atcaatgccg agcctgtcgc aaagtgcatc taaattattc cgtttgccgg ggtacattct
     3541 tcgtgccatt tgcaggctat cggttacggt acacatttct gcggtttttg ccggtggatt
     3601 aggcagataa gaaaattctt gatccataaa cgccacatcg aagggggcat tgtgaataat
     3661 taattccgca cctttgatga aatccaaaaa ctcctcggca atttcagcaa aaacaggttt
     3721 atcttgcaaa aattcattgg tgatgccgtg aactgcaatc gcctcttcat ctacctcacg
     3781 cggtggcttg atataaacgt gataagttct gccggttaaa cggcggttaa tgacttcaac
     3841 cgcaccgatc tcaataatat tatgcccaat ctgtggcggg ccacctgcca tattcattcc
     3901 tgtggtttca gtatccagca ctacttggcg gataattttt tcttctgtca tattcaattt
     3961 aaaattgtag gggtgaaata tttttatccc ttaattctgc atttgattag gggcgaaaca
     4021 tatttcgccc ctacattgtt acttcgcaat ccgtttatat ttcactcggt gcggttgggt
     4081 ggcggcttca ccgaaggttt tcttcttcca ctcttcatag tcggagaagt taccctcgta
     4141 gaacgtcact ttgccttcat cgccgtagtc taaaatatgg gtggcgatac ggtctaagaa
     4201 ccagcggtcg tgcgaaatta ccattgcaca gcccgggaac tctaagatcg cattttccaa
     4261 cgcacgcagg gtttctacgt ctaagtcgtt ggtcggctcg tccagtaaca acacgttacc
     4321 gccacgttgt agtagcttcg ccaagtgtaa acgaccacgt tcaccgcctg aaagctcgcc
     4381 cacacgtttt tgctgatcta cgcctttgaa gttgaaacgc cccacataag cacggcttgg
     4441 gatttcaaag ttaccaatcg tgagaatatc ctgcccgttc gacacctctt cccacacggt
     4501 tttcttatcg tccatcgcat cacggaactg atcgaccgat gccattacca ccgtttcacc
     4561 aagcacgatt gaaccagaat ccggctgttc tttaccagaa agcatacgga acaaggttga
     4621 tttacccgca ccatttgccc cgataatccc gacaatcgcc cccttcggaa tagcgaacga
     4681 caagtcgtca atcaaagtgc gatcgccgta agatttagtc aggttttgta cctcgatcac
     4741 tttatcacct aagcgtggac ccggtggaat aaagagttcg ttagtctcgt tacgtttttg
     4801 atattcgcct gagttaagct cttcaaagcg tgccatacga gccttacttt tcgcttggcg
     4861 accttttgga ttttggcgta cccattccaa ctctttctca atcgattttt gacgagccga
     4921 ttccgccgct tgctcttgtg ccaagcgttt ttctttctgc tccaaccaag aagagtagtt
     4981 accttcccaa ggaatgcctt caccacggtc aagctctaag atccagcccg caacgttgtc
     5041 taagaagtaa cggtcgtggg taattgccac tacggtgcct tcgtagtcgt ggaggaaacg
     5101 ctccaaccac gccaccgatt ccgcatctaa gtggttggtc ggctcatcta acaataacat
     5161 atccggtttt tccagcaaca gacggcaaag tgccacacgg cggcgttcac cccctgataa
     5221 atgctcgatt tttgcgtccc aatccggcag acgtaacgca tcggcagcac gctctaactg
     5281 gttttgaagg ttatggccat cgtgggcttg aataatcgcc tctaattccg cctgttttgc
     5341 cgctaatttg tcgaaatcgg cattctcatc agcatacatc gcatagactt catcaagctg
     5401 ggttaaggcg tttttcactt ccgcaaccgc ttcttccacc gcttcacgca cggtttgctg
     5461 tggggcaagt ttcggttctt gtggaagata gccgatttta attcccggtt gtggacgagc
     5521 ctcgccctcg aactctttat ctacgcccgc cataatgcgt aaaagcgttg atttacccgc
     5581 cccgtttaag ccgagcaccc cgattttcgc ccccgggaaa aagctcaggg aaatatcttt
     5641 aagaatgtga cgcttcggcg gcacgacttt gccgacacgg tgcatcgtat atacaaattg
     5701 agttgacatt ggttgtattc ctcacaaaaa aattcgcctt attttacttg aatttacagc
     5761 caaaatatag ggtggttttg gcttttggta aagtaacctt ttccaataga gatgttattt
     5821 gaggctgcat agtactatgc atttctctta ccttgacagg taagagaaac aaatcctgaa
     5881 ggaaaaaaat tttaaggaag ttattctatg caatatgtat gtccaaaaac actcaaatta
     5941 ttcaacagca ttgttggttg ttataaggga gaagattaat tctctacctc atcaaggctt
     6001 gattttagat ttaatatcca tttctaaaga tgatttagat gatattttgg agggtaatac
     6061 tgatatgaat attcatcaat tattaagcct gatttataag gctgga
//
LOCUS       DANLZC010000139         6079 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.139, whole genome shotgun sequence.
ACCESSION   DANLZC010000139 DANLZC010000000
VERSION     DANLZC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.139"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..633)
                     /locus_tag="PXE44_002148"
     CDS             complement(<1..633)
                     /locus_tag="PXE44_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDL5589618.1"
                     /translation="MAEGLLLTPYFQTEVGIVFYKVPKDVSPAAFGERTLLQPAPTDL
                     ATVKSGKVANFSANSTACKELAVFAKSAKVRSTLNQRDKYSNDLPYIRFVKSIQTCQC
                     RDGKYCHTEKVLVENKNGAVQLCWHHDKMRMDGEIPNEKLEALADENWEKFIAQQIRL
                     TLRKTQSTAIEFADLVLFASIKGLLDELDSKALQQFLGYPPLNNSGVSKES"
     gene            complement(760..1353)
                     /locus_tag="PXE44_002149"
     CDS             complement(760..1353)
                     /locus_tag="PXE44_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5589619.1"
                     /translation="MTDFDKNTYQTPNYVRNWLNHRYAWFHIDGCSNGQNTLYTYWIG
                     RAADGLDDDKLSCQIADDFLADDLFDVLLDKVSDWGELLRIFVNPPYSDPLPFVQRAA
                     ELKKAGHLVVMLLPADKTTEWYTIIQQHANEVIDIIGYHDEKGTWRTGRIQFINPVTG
                     KPAQGNNKGSMIVVFDPFIEGIVTRQMPLDKIKEWGK"
     gene            complement(1364..2410)
                     /locus_tag="PXE44_002150"
     CDS             complement(1364..2410)
                     /locus_tag="PXE44_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDL5589620.1"
                     /translation="MRFTSTINNVRLVEWEINITQGALVDLINQASSWAKAVVIDGIT
                     YYWMSFSKVCEELPAVFSKEDTVYRQYKVLKEKGIIDHFKMDGKDYVRLTEKGCEWNK
                     FEPIRESEKNPSIGNNSEQTRKNFRESSEKNPTDNNTNYKNNNDHTTPLNPPAGEPAP
                     AEVVLNYLNSALVTLAVQLGERKPVGYSLKPWAKNITARIRESSVADCCQVVDYLVAK
                     WGRDEKMREYLCPKTIFRQSNFADYFPKSTAWANNGKPVCVNGKWVTPAELEKRLIMP
                     TVDEVRALFQKSLSGNPFKALDFTNKRNLVMYHATINTRNKRPLERDLPMIISQEIKN
                     AVERIDRLRVPTFS"
     gene            complement(2407..3090)
                     /locus_tag="PXE44_002151"
     CDS             complement(2407..3090)
                     /locus_tag="PXE44_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HDL5589621.1"
                     /translation="MNQLLNISAPKASITMSSREIAELCEKEHRHVTRDIEAMFSQLD
                     IPAEGYAHFWTHPQNGQTYREFRLPYDLTLTLISGYSVILRKKIIDRWQELESRQGIP
                     QSFSQALRLAAEQQEVIEQQNHQLAIQAPKVAFVEHYVEVGTTKSLRETAKILNFPEK
                     RLIECLERYRVLYRQSGNLLPYQDKQAKQLFAVKTGTAEHGHNFTQTRVTAKGIEWIA
                     QRYASELGQ"
     gene            complement(3148..3336)
                     /locus_tag="PXE44_002152"
     CDS             complement(3148..3336)
                     /locus_tag="PXE44_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL5589622.1"
                     /translation="MKKDDVLEHFGTLEKVAATLGISVSAVSQWGEIIPEKNAYRLQE
                     ITAGKLKVKHSLYRNRSK"
     gene            3461..4117
                     /locus_tag="PXE44_002153"
     CDS             3461..4117
                     /locus_tag="PXE44_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5589623.1"
                     /translation="MNTQTLGDRIKSRRKELKLTQKDLATALKGVSHVAVSQWESSTT
                     KPNSENILDLSTVLQCDISWLLRGNGESNVLPASLGMTKVPLISYIQAGQWTGIDDFR
                     ETCGDYEYILTDLEVSEDAFALEIKGDSMEPDFIEGDRVIIDPDVQPHAGEFVAAING
                     DYEATFKKYRPLEDLDEYGRQHFELIALNPDYHKLSTLKQEIRIIGTMVEHRIYRRKR
                     "
     gene            4129..4503
                     /locus_tag="PXE44_002154"
     CDS             4129..4503
                     /locus_tag="PXE44_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589624.1"
                     /translation="MTEFKDVKQAIVEAQLASTPKDKVVNDIMKGSAELLSANDIINR
                     LGIGADEFQELVNLPNHYLRINGGLGAAVYNTRRMFDTHVELVNQELEKNISFPKPDL
                     YILGKARWKKETFKKWLEEQCR"
     gene            4655..5392
                     /locus_tag="PXE44_002155"
     CDS             4655..5392
                     /locus_tag="PXE44_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL5589625.1"
                     /translation="MLSKQVLSISESRLSTYLFCFFRNDPTKQKEAIAIYTALQHRTG
                     IYFSLIQEIEVALRNEVSQLLRFAAPNHNLYEFFHFLANDSNAPLNAESKRQLQKAIS
                     ECGRNYNENDIISHISFGFWVNLFNYDIKRNQYVAYWQSVLKPIFGHRFSNFKDLYNT
                     LKQIMRFRNRLYHQEVVWNKRIAKKPTHALENLEKTYKQFELALQKIAPERFAFRQLS
                     QALIWQRQIFFDQQIFEAEIVILPQHI"
     gene            complement(5428..6024)
                     /locus_tag="PXE44_002156"
     CDS             complement(5428..6024)
                     /locus_tag="PXE44_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589626.1"
                     /translation="MKKLLMTLCCLPAFALANETGESCSKIEDSAKRLECYDSVFVKK
                     DTGKDEATDEKSNWEYEQKKDELRNATTYFAKIRSTNTIDFGFPYNSSSMNLMLRKDP
                     KYGNDVIFSVHGQFNGCMIESCKITVKFDDGKLESYRMIGADGGSNDTLFIENAKAMK
                     TFVDKLKKSKKLIVEASFYNYGKGQFTFDTQGLEWKHF"
BASE COUNT         1789 a         1242 c         1119 g         1929 t
ORIGIN      
        1 actttctttg ctcacaccgc tattattcag tggtggatag ccgagaaact gctgtaatgc
       61 cttgctgtct aattcatcaa gcaaaccttt gatactcgca aacaacacta agtccgcaaa
      121 ttcaatagcg gtagattgcg ttttgcgtaa ggttaagcgg atttgttggg cgataaattt
      181 ttcccagttt tcatccgcta gtgcttctaa tttttcattc gggatttcac cgtccattcg
      241 cattttgtcg tgatgccaac aaagctgtac cgcaccgttt ttattctcta ccagcacttt
      301 ttccgtgtgg caatatttgc catctcggca ttggcacgtc tgaattgact ttacaaaccg
      361 gatataaggc aaatcattac tatatttgtc acgctgattt agcgttgaac gaacttttgc
      421 cgatttagca aacaccgcca gctctttaca agcggtcgaa tttgccgaaa aatttgcaac
      481 cttgcctgat ttaaccgtcg ctaaatcggt cggtgcaggt tgcaacaaag ttcgctcacc
      541 gaaagcagca ggagaaacat ctttaggcac tttgtaaaac acaataccca cttcggtttg
      601 aaagtaaggg gtaagtagca aaccttccgc cattattgcc cccatttctc ggtttcatca
      661 taggctttca cgaatagcat aaatacgcca atacagatta aaaaaatcag tacgccaatt
      721 actgcaccaa taccaataaa tagttcccaa taatccattt tatttacccc attccttgat
      781 tttatctagt ggcatctgac gagttacaat gccctcaata aatgggtcaa acacaacaat
      841 catcgagcct ttattattac cttgtgccgg tttacctgtt accgggttga taaattgaat
      901 ccgccctgtc cgccaagtgc ctttttcatc gtgatagcca ataatatcaa ttacctcatt
      961 agcgtgttgt tgaatgatgg tgtaccattc tgttgtttta tccgctggta ataacatcac
     1021 aaccaaatga cctgcttttt tcaattctgc cgctcgttga acaaaaggca gtggatcaga
     1081 atatggcgga ttaacgaaaa tacgcagtaa ttctccccag tctgaaactt tatctagtag
     1141 cacatcaaat aaatcatcgg ctaaaaaatc atcggcaatt tggcaactta atttatcatc
     1201 atctaaaccg tctgccgctc taccaatcca ataggtgtac aacgtatttt gtccgttaga
     1261 gcaaccgtca atatgaaacc aagcataacg atgatttagc caattacgca cataattagg
     1321 tgtttggtaa gtgtttttat caaaatctgt cattgtctaa tccttacgaa aatgttggta
     1381 ctctcaaacg gtcgatacgc tcaaccgcat ttttaatttc ttggctgata atcatcggca
     1441 aatcacgttc aagtggtcgc ttgtttctgg tgttgattgt ggcgtggtac atcactaaat
     1501 ttcgtttgtt ggtaaagtcc aatgccttaa acggattgcc actgagtgat ttctggaaca
     1561 aggctcgtac ttcgtccacc gttggcataa tcaagcgttt ttccagctcc gccggtgtaa
     1621 cccacttacc atttacgcaa acaggctttc cgttattagc ccaagcggtc gattttggaa
     1681 aataatctgc aaaattcgat tggcggaaaa tcgttttcgg gcagaggtat tcacgcattt
     1741 tttcatctcg tccccattta gcgacaaggt aatccaccac ttggcaacag tccgccaccg
     1801 aactttcacg aatgcgagca gtaatatttt ttgcccacgg cttgagcgaa taccccacag
     1861 gcttacgctc accaagctgt accgccaacg ttaccaaagc cgaattgaga taattcaaca
     1921 caacttcagc cggggcaggt tcccccgctg ggggattaag gggggtagta tgatcattat
     1981 tatttttata attagtatta ttatctgtcg gatttttttc cgagctttct cggaaatttt
     2041 tccgagtttg ttcggagtta tttccgatac tcggattttt ttccgactca cggatcggct
     2101 cgaatttatt ccattcacaa cctttttcgg ttaaacggac ataatcttta ccgtccattt
     2161 tgaagtggtc gataatgcct ttttctttca acaccttata ttggcggtaa actgtatctt
     2221 ctttgctaaa aaccgcaggt aactcttcgc aaaccttgct aaatgacatc cagtaatagg
     2281 ttatgccgtc aataaccacc gctttcgccc aactggaggc ttgattgatt aaatcgacca
     2341 atgccccttg tgtaatattg atttcccatt ccactaagcg gacattgttt atggttgatg
     2401 taaatctcat tgtcctaact ccgatgcgta acgttgagca atccattcaa tccctttggc
     2461 ggttactctc gtttgggtaa aattatgacc gtgttctgcc gtgccggttt ttactgcaaa
     2521 aagctgttta gcttgtttat cttgataagg gagtaggttt cctgattggc gataaagaac
     2581 tcgatatcgt tctaaacatt caatcaaccg cttttctgga aaattgagta tcttggcggt
     2641 ttctcgcaat gatttagtcg ttcccacctc aacataatgc tccacaaatg cgactttcgg
     2701 ggcttgaatg gctaattgat gattctgctg ctcaatcact tcttgctgtt ctgccgccaa
     2761 ccgtagagct tgagagaaag attgtggaat accttgtctg ctttctagct cttgccaacg
     2821 gtcgataatt ttcttgcgaa gtatcacgct atagccggaa attagggtaa gggttaaatc
     2881 gtaaggcaat cgaaattccc ggtatgtttg cccattttga gggtgtgtcc aaaaatgggc
     2941 atacccctcg gcaggaatat ctaattgact aaacatcgct tctatatcac gagtaacgtg
     3001 tcgatgttct ttttcgcaca actcagcaat ctcacgacta ctcatcgtaa tgcttgcttt
     3061 tggtgccgaa atgtttaata attgattcat cgaaaatacc tttcgtgttt aattatgtat
     3121 gaaagccacc gttacagcgg tggtttttta tttgcttcta tttctataaa gtgagtgttt
     3181 gacctttaac ttgccggcag tgatctcttg taatctgtac gcatttttct caggaataat
     3241 ctcgccccat tgagaaacag cagatacact aatccctaaa gttgccgcca ctttttctag
     3301 agttccgaaa tgttcgagca catcatcttt tttcatattt tcttaattcc tttcttgaat
     3361 tttatttaag acctcttaaa tatacaatta aaggaaactt aagtcaataa atgttaagat
     3421 aacttaacag caactttatt tttaccttta aggggatact atgaatactc agaccttggg
     3481 cgatagaatt aaaagccgta gaaaagaatt aaaattgaca cagaaagatc ttgctactgc
     3541 gttaaaaggg gtctcacacg ttgcagtatc tcaatgggaa agtagtacga ctaagccaaa
     3601 ttcagagaat attttggatt tatctacagt attgcaatgc gatattagtt ggctcttgcg
     3661 tggtaatgga gagtctaatg ttctacctgc tagcctcggt atgactaaag tcccattaat
     3721 tagctatatt caagccggac aatggacggg aattgacgat tttcgtgaaa cctgtgggga
     3781 ttatgaatat atcctaactg atcttgaggt gtctgaagat gcttttgccc tagaaatcaa
     3841 aggggactcg atggagcctg attttattga aggagaccga gtaattattg atcctgatgt
     3901 acaacctcac gccggtgagt ttgtggcagc aattaatggc gattatgaag ccactttcaa
     3961 aaaataccgt ccgttagaag acttagacga atacggaaga caacattttg agcttatcgc
     4021 tcttaatccg gattaccata aattatcaac gctaaagcaa gaaatcagaa ttatcggcac
     4081 aatggtagag catcgtattt atcgtagaaa gagataggaa ttgataatat gacagaattt
     4141 aaagatgtaa aacaggcgat tgtagaagct caattagctt cgacacctaa agataaagtc
     4201 gttaatgaca taatgaaagg aagtgcggaa ctgctttcag caaatgatat tatcaatagg
     4261 ttaggtattg gggcagatga gtttcaagag ttggttaatt tacctaacca ttatttgcgt
     4321 ataaatggag ggttaggtgc tgctgtgtat aatacaagaa gaatgtttga tacccatgtt
     4381 gagttagtca atcaagagtt agaaaagaat atttcatttc ctaaacctga tttatatatt
     4441 ttggggaaag ctcgttggaa aaaagaaaca tttaagaaat ggttggaaga acagtgtaga
     4501 taaagaaaag gtaggttaaa aaacctacct tttctgaacc gcaaataagc gattctcaca
     4561 ctttacaaag caatccagct cctacgtctg gggcttcggg tccgttgcca gtgcaagtat
     4621 tattacatta aactaaactt gagtaaagga aaatatgttg tccaaacaag tgctaagtat
     4681 ttcagaaagt cggctgagta catatttatt ttgctttttc cgaaatgatc ccacaaaaca
     4741 gaaagaagct attgcaattt ataccgcctt gcaacatcga acaggaattt atttttcatt
     4801 gattcaagaa atagaggtgg cactaagaaa tgaagtcagc cagttgttac gctttgctgc
     4861 acctaatcat aatttatacg aattctttca ttttctagcg aacgatagta atgctcctct
     4921 taatgcagaa tctaaacgcc agctacaaaa agccattagt gaatgcggac gtaactataa
     4981 tgaaaatgac ataatttctc atatttcttt tggattttgg gtaaatctgt ttaattatga
     5041 tataaagcgt aatcaatatg ttgcttactg gcaaagcgta ttaaagccta tttttggaca
     5101 tagatttagt aattttaagg atttatacaa tacattaaag caaattatgc gttttagaaa
     5161 tcggctgtat catcaagaag tggtttggaa taagcgaatt gccaagaaac caacgcacgc
     5221 actagagaat ttagaaaaaa cttacaagca atttgagtta gcactacaaa agatagcacc
     5281 tgaacgattt gcgtttcgtc agctttcaca agcacttatt tggcaacgcc aaatcttttt
     5341 cgaccaacaa atttttgaag ctgaaatcgt tattttgccc caacatatct aagcaaaaag
     5401 ctacgcatct atgtagcttt ttcttaatta aaaatgtttc cactctaacc cttgtgtatc
     5461 aaaggtaaat tgccctttgc cataattgta aaaactcgcc tctacaatta gttttttcga
     5521 ctttttcaac ttatctacaa aggttttcat tgcttttgca ttttcaatga aaagcgtatc
     5581 attgctgcca ccatccgccc caatcattcg ataactttcc aatttaccat catcaaactt
     5641 cacagtaatt ttacagctct caatcataca gccattaaat tgaccgtgta cgctaaaaat
     5701 tacatcattg ccatatttag ggtctttgcg taacattaaa ttcattgatg atgaattata
     5761 cgggaaacca aaatctattg tgttagttga tctaatttta gcaaagtaag tcgttgcatt
     5821 acgcaattca tctttctttt gttcatattc ccaattagac ttttcatcgg tagcttcatc
     5881 tttaccagta tcttttttca caaagacact atcataacat tctaagcgtt ttgcactatc
     5941 ttcaatctta gagcaacttt ctccagtttc gttagctaaa gcaaaagcgg gcaaacaaca
     6001 taaagtcatc agtagttttt tcatataacc tccatagaaa tcccttctta ttttctcaaa
     6061 agcgtggttt tcggtctgt
//
LOCUS       DANLZC010000140         6071 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.140, whole genome shotgun sequence.
ACCESSION   DANLZC010000140 DANLZC010000000
VERSION     DANLZC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.140"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(36..413)
                     /locus_tag="PXE44_002157"
     CDS             complement(36..413)
                     /locus_tag="PXE44_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HDL5589627.1"
                     /translation="MKNPIEIYQSQDGKTQVSVQFDKETVWLSLQQMADIFGRDKSVI
                     SRHLRNIYQEGELEREATVAKNATVQIEGAREVVRLKDYLVKGYALNERRLQERGIEF
                     EQVIGLLSQYTGCLSLVGGRKFA"
     misc_feature    complement(435..557)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(687..1676)
                     /gene="rsmC"
                     /locus_tag="PXE44_002158"
     CDS             complement(687..1676)
                     /gene="rsmC"
                     /locus_tag="PXE44_002158"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDL5589628.1"
                     /translation="MLSLESEVLERHLALFENKSVLLFGDVRDDFARQLQSRAEKVAV
                     FSSYFDYARHHSEVEFGLECGLSAELAVFYWTKNKQECQFQLLQWLSQCQAGQEMLII
                     GENRAGVRSVEKLLEPFGNIAKIDSARRCGLYHFELQTVPTFDCKKFWKSYRLQDLDV
                     FDLPAVFSSAELDNGTKLLLSTFDKADRLKGKVLDLGCGAGVIGATLKQQFPKIKLTM
                     SDIHAMALESGRRTLAENQLEGEVVASDVFSHINERFDLIVSNPPFHDGVDTAYRAVE
                     ELIFQAKNHLTKGGELRIVANAFLPYPDLLDKAFGKHDVLAKSTKFKVYSART"
     gene            1806..2228
                     /locus_tag="PXE44_002159"
     CDS             1806..2228
                     /locus_tag="PXE44_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDL5589629.1"
                     /translation="MNRRDLLLQEMGISQWVLTNPQVLKGDAQIRLNNAVKLVVVCEE
                     DHQHSRLFADILRALKLKNSEYQWLTGEQSQRLVFEHSPIFWLIQAEEQAVKMAKSFT
                     NQTAWKNESWQDLAKSTTKRQLWQQIDTFSAQFGEQRD"
     gene            2221..2658
                     /gene="rimI"
                     /locus_tag="PXE44_002160"
     CDS             2221..2658
                     /gene="rimI"
                     /locus_tag="PXE44_002160"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL5589630.1"
                     /translation="MIEQIQAEDFEQLFEIEQQAHLVPWSKGTLLNNQGEKYLNLKVV
                     EQNKIVAFAISHIVLDEATLFNIAVAPDFQGKGIGKRLLSELILQLQQRKLTTLWLEV
                     RESNHSARKLYDSLGFNEVTTRKNYYPTPNGSKENAVVMALYL"
     gene            complement(2707..4554)
                     /gene="typA"
                     /locus_tag="PXE44_002161"
     CDS             complement(2707..4554)
                     /gene="typA"
                     /locus_tag="PXE44_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL5589631.1"
                     /translation="MQNVDISKLRNIAIIAHVDHGKTTLVDKLLQQSGTFGETRGDVD
                     ERVMDSNDLEKERGITILAKNTAINWNGYHINIVDTPGHADFGGEVERVLSMVDSVLL
                     VVDAFDGPMPQTRFVTQKAFAHGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLGASD
                     EQLDFPIIYASALNGVAGLEHEDLAADMTPLYEAIVKHVQPPQVELDAPFQMQISQLD
                     YNNYVGVIGIGRIKRGSVKPNQAVTIVDSEGKTRQGRIGQVLGHLGLQRFEATEAFAG
                     DIVAITGLGELNISDTICDINAVEALPALSVDEPTVTMFFCVNTSPFCGQEGKFVTSR
                     QILERLNKELVHNVALRVEETSNPDEFRVSGRGELHLSVLIENMRREGYELAVSRPKV
                     IYREEDGKKQEPFEQVTIDIEEQHQGSVMEALGIRKGEVRDMIPDGKGRTRLEYVIPS
                     RGLIGFRNEFMTMTSGTGLLYSTFSHYDDVKPGEIGQRKNGVLISNATGKALAYALFG
                     LQERGKLMIDHGVDVYEGQIIGIHSRSNDLTVNCLQGKKLTNMRASGKDDAIVLTTPV
                     RFTLEQALEFIDDDELVEVTPQSVRIRKRLLTENDRKRASRTTTSTSTH"
     gene            complement(4739..5416)
                     /locus_tag="PXE44_002162"
     CDS             complement(4739..5416)
                     /locus_tag="PXE44_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL5589632.1"
                     /translation="MSIAENLSQIHQQIQQISEQYQRDNVRLLAVSKTKPVEAIQAAI
                     EAGQRAFGENYVQEAVEKIEFFANRPELEWHFIGPLQSNKTKLVAAYFDWIQTVDRLK
                     IAERLNEQRPADKAPLNVLIQINISDEASKSGIEPDEMLPLAQAISQLPNLKLRGLMA
                     IPKPESEPEQQKVAFRKMQLLFNRLQTEFDDIDTLSMGMSDDMQAAIECGSTMVRIGT
                     AIFGARR"
     gene            5487..6026
                     /locus_tag="PXE44_002163"
     CDS             5487..6026
                     /locus_tag="PXE44_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDL5589633.1"
                     /translation="MLDLTKIKLVITDVDGVLTDGGMYYTDQGEVMKRFHVHDGLGVK
                     MLQSCGIKVAVLSGGDTALLRKRLEVLKIDLALLGKMEKRSACFELMQQAGVTPEQTA
                     YIGDDTLDLPAFEVCGIAIATRNALDYIKEKADWVLQKAGGEGAFREVSDQILEAQGF
                     GEIFKTADGFLTVAGKMAQ"
BASE COUNT         1678 a         1352 c         1273 g         1768 t
ORIGIN      
        1 agtgttcaat cagtttaatc atcagttctt tttgttcagg caaactttct gccaccaaca
       61 aggctaagac aaccagtgta ttgactgagc aaaccaatca cctgctcaaa ctcgatacct
      121 cgttcctgca aacggcgttc gttaagggca tagcctttca ccagatagtc ttttaggcga
      181 acgacttctc tagcaccttc aatttgaact gttgcatttt ttgcaacagt tgcctcacgc
      241 tccagttcac cttcttggta gatatttcgt aagtgccgag aaataacaga cttatctcgt
      301 ccaaaaatat ctgccatttg ctgtaaactt agccacaccg tttccttatc aaactgcacg
      361 gaaacttggg ttttgccgtc ttggctttgg taaatttcaa tcgggttttt catattattc
      421 ccaaatttta tctataaaaa aatctctcga aagttgcctt ccgagagatc tttattgatt
      481 tgctacactt gctcggaaga gttatcttcc gcacacacca aatgcccgaa agatatcagg
      541 ttaaagtgtg gtggtgatgt aaagtgtagt ttaaattcat tgcattactc caaaacagta
      601 tgaacagaat ttacgtcaag ccggcagaaa tatcaagcac ttttttaagc aaataccggc
      661 ttttttataa ccgtttgttc tgcctattat gttcttgcgg aatacacctt aaacttggtg
      721 gattttgcca gtacgtcgtg tttgccgaag gctttatcga gcaaatctgg gtaaggcaag
      781 aatgcgtttg cgacaattcg cagctcaccg cctttggtta ggtgattttt agcttggaaa
      841 atcaactcct ccaccgctcg gtaggcggta tcgaccccgt cgtggaatgg tgggttggag
      901 acaattaaat caaaccgttc attaatgtga gagaacacat cacttgctac cacttccccc
      961 tctaactgat tttccgccaa agtacgtctg ccggattcca acgccattgc gtgaatatcg
     1021 ctcatcgtca gtttgatttt cggaaattgt tgtttgagcg ttgccccaat cacgccagcc
     1081 ccacagccta aatctaacac tttgcctttt agcctgtctg ctttgtcgaa ggttgaaagc
     1141 aataatttgg tgccgttatc cagctccgcc gagctaaaaa ccgctggtag atcgaacaca
     1201 tctaagtctt gcaagcggta ggatttccag aattttttgc aatcgaatgt cggcacggtc
     1261 tgtagctcaa aatggtataa accacaacgg cgtgcagagt cgattttggc aatattgccg
     1321 aacggttcga gcagtttttc taccgaacga acaccagctc ggttttcgcc gataatcaac
     1381 atttcttgcc ctgcttggca ttgcgacagc cattgtagca gttggaattg gcattcctgc
     1441 ttgttcttcg tccaataaaa caccgctaac tcggcactca aaccacattc taagccaaat
     1501 tcaacctcac tatggtggcg tgcgtaatca aaatagctac tgaataccgc aaccttttcc
     1561 gcacgagatt gcagctggcg ggcaaaatca tctcgcacat cgccaaataa taacaccgac
     1621 ttgttttcaa ataacgccaa atggcgttca agtacttcgc tttctaaaga gagcattttc
     1681 ttaccttatc taaaaaattt agcgtgaata taccgaattt aagtacagaa atcttgaatt
     1741 ttttgaaaaa agtttctgtt caagcggagt tttgatacta tttgcaaaat ttgatgaggt
     1801 tttaaatgaa tcgtcgtgat ttactgttac aggaaatggg tatttcgcaa tgggtgctaa
     1861 ccaatcccca agtgctaaaa ggcgatgctc aaatccgcct aaacaatgcg gtaaaattgg
     1921 tggtagtgtg tgaggaagat caccaacaca gccggctctt tgccgatatt ctgcgtgctt
     1981 taaagttaaa aaacagcgaa tatcaatggc tgactggcga acaatcacaa cgtttggtgt
     2041 ttgagcattc gccgatcttt tggctgattc aagcggaaga acaagcggtc aaaatggcaa
     2101 aaagttttac taatcagacc gcttggaaaa acgagtcttg gcaagattta gccaaatcta
     2161 cgaccaaacg ccaactttgg caacaaattg atactttttc ggcacaattt ggagaacagc
     2221 gtgattgagc aaattcaagc ggaagatttc gagcagttat ttgaaattga gcaacaagcc
     2281 catttagtgc cgtggtcaaa ggggacgtta ttaaataatc agggtgaaaa atacctgaat
     2341 ttaaaagtgg tagagcaaaa caaaattgtg gcttttgcca ttagtcatat cgtgctagat
     2401 gaagcaacct tatttaacat tgcagttgct cctgattttc aaggcaaagg cattggcaag
     2461 cggttacttt ccgaattaat tttgcaactg caacaacgga aactcaccac cctgtggttg
     2521 gaagtgcgag aatccaacca ttctgccaga aaactctacg attcactcgg ttttaatgaa
     2581 gttaccaccc gcaaaaacta ttaccccacg ccaaacggta gcaaagaaaa tgcggtggta
     2641 atggcgttat atttgtaggg agaatttaaa accaaaagag cgacctcagt cgctcttttt
     2701 tatcagttag tgcgtgctgg tactggttgt agtacggctt gcacgtttcc ggtcgttttc
     2761 agttaataaa cgtttacgaa tacgcaccga ttgtggtgtt acttccacta attcgtcatc
     2821 gtcaataaac tctaacgctt gttctagagt gaaacgcacc ggtgtggtta aaacgatggc
     2881 atcgtcttta cctgatgcac gcatattggt taatttctta ccctgtaagc agtttacggt
     2941 taaatcgttt gaacggctgt gaatcccgat aatttgccct tcatatacat caacaccgtg
     3001 gtcgatcatt aattttccac gctcttgtaa gccaaataat gcgtatgcta atgccttacc
     3061 cgttgcgtta gaaattaaca caccgttttt acgctgaccg atttcgcccg gttttacgtc
     3121 atcatagtgg ctgaatgtag agtaaagtaa accagtacct gaggtcatcg tcataaattc
     3181 gttacggaaa ccgattaagc cacggcttgg gatcacatac tctaaacggg tacgaccttt
     3241 gccgtccgga atcatatcac gcacttcacc tttacggata ccaagtgctt ccatcactga
     3301 accttggtgt tgctcttcga tatcaatcgt cacttgctca aacggctctt gttttttgcc
     3361 gtcttcttcg cggtagatta ctttcgggcg agacactgct aactcgtaac cttcacgacg
     3421 catattttcg attaataccg ataagtgtaa ttcgccacga cctgacacac ggaattcgtc
     3481 tgggttcgaa gtttcttcca cacgcaatgc cacgttgtgg actaattctt tgtttaaacg
     3541 ctcaagaatt tggcgtgaag tcacaaattt accttcttga ccgcagaatg gcgaggtgtt
     3601 tacgcagaag aacatcgtta ccgttggttc atcaacgctt aatgctggta aagcctctac
     3661 tgcgttaata tcacaaatag tatctgaaat atttaattcg cctaaaccag taatcgcaac
     3721 aatatcgcca gcaaaggctt ctgttgcttc aaaacgttgt aaacctaaat gccccaacac
     3781 ctgaccgata cgaccttggc gagttttgcc ttcgctatct acaatcgtta ccgcttggtt
     3841 aggtttcacc gaaccacgtt tgatacgtcc aataccgata acgcccacat agttgttgta
     3901 gtctaattgc gaaatctgca tttggaatgg tgcatctaat tccacttgtg gtggttgaac
     3961 gtgtttgaca atcgcttcat aaagtggggt catatctgcc gctaggtctt cgtgttctaa
     4021 gcctgctaca ccatttaagg ctgaagcgta gataattggg aaatctaact gctcatcact
     4081 tgcacctaaa tttacaaaca agtcgaacac ttgatccacc acccaatcag ggcgagcacc
     4141 tgggcggtca actttattga tcactacgat tggttttaaa ccgtgagcga acgctttttg
     4201 cgtgacgaaa cgggtttgcg gcatcgggcc atcaaaggca tcaaccacta acagaacaga
     4261 atctaccatt gaaagtacac gctcaacctc accaccgaag tcagcgtgcc ctggggtatc
     4321 tacgatattg atgtgataac cattccagtt aattgccgta ttttttgcaa gaatggtaat
     4381 gccacgctct ttttcaagat cgttggaatc cattacacgc tcatcaacgt caccacgagt
     4441 ttcaccaaag gtgcctgatt gttgtaaaag tttatcaacc aaggtggttt taccgtggtc
     4501 aacgtgggca ataattgcaa tgttacgcaa ttttgaaata tctacatttt gcattggaat
     4561 atctttaagt taagtcaaaa taaaaactct cgcccatcaa aactggcgag agtcaagaaa
     4621 ttaggaggcg taattataca gactttttcc gctgttggct acaaaacaag cggtggaatt
     4681 tgcaaagttt ttggcgaatt tgaccgcttg tatcagcttt atgtaaaaac tcagctggct
     4741 aacgtctcgc cccaaaaatc gcagtcccaa tccgcaccat tgtcgagcca cattcaatcg
     4801 ccgcttgcat atcgtccgac atccccattg aaagggtatc aatgtcgtca aattctgttt
     4861 gcaagcggtt aaaaagtagc tgcattttgc gaaaggcaac tttttgttgc tctggctcgc
     4921 tttctggttt gggaatggcc attaagcccc gcagtttcaa attcggtaat tgagaaatcg
     4981 cttgggcaag tggtagcatt tcatctggtt caatgcccga tttcgaggct tcatcgctga
     5041 tattaatttg aatcagcacg ttgagcgggg ctttatccgc tgggcgttgt tcgtttaaac
     5101 gctcggcaat ttttaatcta tccaccgttt gaatccaatc aaaataggca gcgaccaatt
     5161 tggttttatt tgattgaagt ggcccaataa aatgccattc taattcgggg cgatttgcaa
     5221 aaaattcgat tttttcgacc gcttcctgca cataattttc gccaaatgca cgctgccctg
     5281 cctcaatcgc cgcttgaatc gcctcaactg gtttagtttt ggagacggct aacaaacgca
     5341 cgttatctcg ttgatattgt tcggaaattt gctgaatttg ttgatggatt tgcgataaat
     5401 tctcggcaat cgacatttcg gttcctttgg taaacggagg taaaaaagct ataatggtaa
     5461 aatcatacca tatttgaggc aaaaaaatgt tagatctcac caagattaaa ttagtgatta
     5521 ccgatgtgga cggcgtgcta accgatggcg gaatgtacta caccgaccaa ggcgaagtaa
     5581 tgaaacgttt ccacgttcac gatggtttag gcgttaagat gttacaaagt tgtggcatta
     5641 aagtggcagt actctcgggc ggcgatacgg ctttgctccg caaacggtta gaggtgctga
     5701 aaattgatct cgctttgtta ggcaaaatgg aaaaacgctc cgcctgcttt gagctaatgc
     5761 agcaagccgg tgttacccct gagcaaacgg cttacattgg tgatgacacc ttagatttac
     5821 cggcttttga agtgtgtggc attgcgattg caacccgtaa cgccctcgat tacatcaaag
     5881 aaaaagcaga ttgggtattg caaaaagccg gtggtgaagg ggcgtttcgt gaagtctccg
     5941 accaaatttt ggaggctcaa ggattcggtg aaatttttaa aaccgcagat ggttttttaa
     6001 cggtagcggg gaaaatggct cagtagactc ttcaagcagt taaatttcat tttatttttg
     6061 cgatctgcat c
//
LOCUS       DANLZC010000141         5800 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.141, whole genome shotgun sequence.
ACCESSION   DANLZC010000141 DANLZC010000000
VERSION     DANLZC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.141"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            25..498
                     /gene="folK"
                     /locus_tag="PXE44_002164"
     CDS             25..498
                     /gene="folK"
                     /locus_tag="PXE44_002164"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDL5589634.1"
                     /translation="MQTVYIALGSNLENPLEQLKQAVEKLKKFANEFEISPFYGSKPV
                     GPQDQPDYVNAVAKFSTDLSPTALLDKLQSIENEQGRVRVRRWGERTLDLDIILYGNQ
                     QIQTERLNVPHIEMKNREFVIVPMFDLTPDLVLPTGEKLADIYAQFKDHQMIIFS"
     gene            complement(495..689)
                     /locus_tag="PXE44_002165"
     CDS             complement(495..689)
                     /locus_tag="PXE44_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDL5589635.1"
                     /translation="MKPATFYFAIFLLNLTACTPKVQLETPSEGITINMNVVVDHRID
                     VKMDEKSRAVITKSENKTKN"
     gene            complement(707..2482)
                     /locus_tag="PXE44_002166"
     CDS             complement(707..2482)
                     /locus_tag="PXE44_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HDL5589636.1"
                     /translation="MPLKTAIRISLLSQNSWGKGNIVISNSDGEITEKGLNLPLRLTG
                     NIKHGNFILYSSVPLDFQGNFDDLSVKFLPSSLLRLTGKERYLTIEDLRFPLAGIKID
                     KHGIHGRLHAILRGQSPDFNNIEFHLDGQAQNFKAGALDFFQDPKDANAVKDSWKWRI
                     WGASILKAFNSKVNLSGRGQWHKQLVRLEELKGDLATVKLADTTISKITLNNTQAIRF
                     NVKKFTLDGAVMLQSPEIAFSYGGVLPKPRVNLAFDGEVEKLRFKGAVNTTELGPLKL
                     FARRQLSQDASQIIGKLYWPEQSAQGFQPLFPFRSQWVINNGTIRGETAFSAGSKNGL
                     IAGGHLAIRNGGLSLPNGEAKGIEFSLPYRYQNGRFHFGAKKPLDVKIAQIKLGDLAL
                     DNASMKLNGYYPYTKAKPLNLNQLSFDLFGGKLNVKRFALPQTELAYLNLERIDFEQI
                     LQFMQYQQIDLKGKANAILPFWLSGKPCYICDGLLTQAETSYLTFTPELLSEMQKSGY
                     TEQILLHLVDKSTINDFRSLINVGPKGDLVLDGKLKLSSNQNKSKVNLNYNHRENLFD
                     LWHLINYGSQFEQKLENYLYQKLER"
     gene            complement(2668..3414)
                     /locus_tag="PXE44_002167"
     CDS             complement(2668..3414)
                     /locus_tag="PXE44_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589637.1"
                     /translation="MKRALKFFGIATVLSVVAIYYFLMPSHYLATPINWILPKAWKVQ
                     LPQGLNLNWQGSSLAQFQLSYRDCPLLSADKTDLGWIEKNHLSVDKLDIDYRCLALFP
                     QNEHSNSDTSLNALLAVIPDGEATIHALNWKNLPTDFNPRLQRLFEQTAIKLSLSKGE
                     IQATAAQQAVKFAANFANGRLNADLSYQPNEKEQHNLRISAEIEDNFTQLPPTISAEY
                     DWQLSYEIIANKALQKGALFSVGKKMPHKS"
     gene            complement(3418..4233)
                     /locus_tag="PXE44_002168"
     CDS             complement(3418..4233)
                     /locus_tag="PXE44_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589638.1"
                     /translation="MAHDHDSPSKLEISTHAALPMVLMLFIEMALNFYSAPKNSIFIS
                     PYLLAFFVTSLISFLVLWKGEICRGQASRLSFILPFLLIFAVGNFLYSIGFTPKHNPM
                     AVASIAAVFACLIYFRLPEDQTLYNTLMYCGLAISSIGIIQYLAIYWFELPSLFNGIR
                     ANNFAQVLLGILLAGWYLMLAKSRLDVFLKLLVKTALVVLILNYCWTAFVLYQHLQIM
                     PDMPLLSFIIYFAAQFVILLMLAWLLLGKNIKNPTAWTVATLLAILYPFANVV"
     gene            4323..4730
                     /gene="gloA"
                     /locus_tag="PXE44_002169"
     CDS             4323..4730
                     /gene="gloA"
                     /locus_tag="PXE44_002169"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL5589639.1"
                     /translation="MRILHTMLRVGDLERSIKFYTDVLGMRLLRRSENEQYKYSLAFL
                     GYADESESAVIELTYNWGVDKYELGTAYGHIALGVDDIYKTIEDVRAAGGKITREPGP
                     VLGGTTVIAFAEDPDGYKIEFIENKDAKAALGN"
     gene            4815..5462
                     /gene="rnt"
                     /locus_tag="PXE44_002170"
     CDS             4815..5462
                     /gene="rnt"
                     /locus_tag="PXE44_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDL5589640.1"
                     /translation="MEQTTEQIDYKLLKHRFRGYLPVVIDVETAGLNAQTDALLELAA
                     ITLKMDEQGYLVPDQQCHFHIAPFEGANINPESLKFNGIDIDNPLRGAVAENIAIPEM
                     FKMVRKAMKEQGCQRAVIVAHNAAFDQGFLQAAIKRINAKRDPFHPFAMFDTATLAGF
                     MYGQTVLVKACQVAKIPFDGKQAHSALYDTERTAELFCAMVNRLKDLGGFPPLSD"
BASE COUNT         1715 a         1105 c         1258 g         1722 t
ORIGIN      
        1 ttgttatatt tgtaagtata ttttatgcaa accgtctata tcgccttagg atcaaacctt
       61 gagaatccgc ttgagcagct taaacaagcg gtcgaaaaat taaaaaaatt tgcaaatgag
      121 tttgaaatca gcccgttcta cggctcaaag cctgtcggac cgcaagatca gcctgattat
      181 gtcaatgcgg tagcaaaatt tagtaccgac ctatcgccca ctgcattgct ggataaattg
      241 caaagcattg aaaatgagca gggaagagtg cgtgtacgcc gttgggggga gagaacttta
      301 gatctggata ttattttgta cggcaatcag caaattcaaa ccgagcgttt gaacgtgccg
      361 catatcgaga tgaaaaatcg ggaatttgtg attgtgccga tgtttgattt aacgcctgat
      421 ttagtactac cgacagggga aaaattagcc gatatttatg cacaatttaa agatcatcaa
      481 atgattatct tcagctaatt tttcgttttg ttctcacttt tggtgatcac cgctcgggat
      541 ttctcgtcca tcttcacatc aatgcggtga tcaacgacca cattcatatt aatcgtaatg
      601 ccttcacttg gcgtttcaag ctgtactttc ggcgtacaag cggtcaaatt cagtaaaaat
      661 attgcaaaat aaaatgtggc cggtttcata tttattccat tctttatcat cgttccagtt
      721 tttgatagag gtaattttct aatttttgtt caaattgtga gccatagtta attaagtgcc
      781 acaaatcaaa cagattttct cggtgattat agtttaaatt cacttttgat ttattttgat
      841 tggaagataa ttttaatttt ccgtctaata ctaaatcgcc tttcggcccg acattaatca
      901 gactgcgaaa atcgttaatc gtgcttttat ctactaaatg taacaaaatt tgctcggtgt
      961 aaccactttt ctgcatttct gacaacaatt caggggtaaa ggttaaatag gaagtttccg
     1021 cctgtgtcag taagccatcg caaatatagc aaggttttcc gcttaaccaa aacggcaaga
     1081 tagcgttggc tttgcctttg agatcaattt gctgatattg cataaattgc aaaatttgct
     1141 caaagtcaat ccgctctaaa ttcaggtaag ccagttcggt ttgtggtaag gcaaagcgtt
     1201 ttacattcag tttaccgcca aacaaatcaa aggataattg atttaaattc aatggcttag
     1261 ctttggtata agggtagtaa ccgttgagtt tcattgaagc gttatctaat gctaaatccc
     1321 ccagtttgat ttgggcaatt tttacatcaa gcggtttttt ggctccgaaa tgaaagcgtc
     1381 cgttttggta gcggtaggga agcgaaaatt caatcccttt tgcttcgcca ttaggtaaag
     1441 ataaaccgcc gttacgaatg gctaaatgcc caccggcaat cagcccgttt ttactgccgg
     1501 cactaaacgc cgtttcgccc cgaattgtac cgttgttgat cacccattgg ctacgaaacg
     1561 gaaaaagcgg ctggaagcct tgagcagatt gctccggcca ataaagtttg ccgataattt
     1621 ggctggcatc ttggcttaat tggcggcggg cgaacagttt gagtgggccg agttcggtgg
     1681 tattcaccgc tcctttaaaa cgtagttttt cgacttcacc atcaaaggcg agattcactc
     1741 gtggtttggg tagcacaccg ccataactga aggcaatctc aggtgattgc aacattaccg
     1801 ctccgtcaag ggtgaatttt ttgacattaa atcgaatagc ttgcgtattg ttaagcgtga
     1861 ttttagaaat ggttgtatct gctaacttta cggtggctaa atcgcctttg agttcttcca
     1921 accgcacaag ctgtttatgc cattgccctc tgccgcttaa attcacttta ctgttgaagg
     1981 ctttgaggat agatgctccc caaattcgcc atttccaact gtcttttact gcgttagcat
     2041 ctttaggatc ttggaaaaaa tctaaggctc cagccttaaa attttgtgct tgtccgtcta
     2101 aatggaactc gatattgtta aaatcagggc tttgccctct taaaattgcg tgcagtcgtc
     2161 cgtgaatacc gtgcttgtca attttaattc cggcaagtgg aaaacgtaaa tcttcaatgg
     2221 ttaaataccg ctcttttccg gtcaaacgaa gtagggagct aggcaaaaat tttacgctta
     2281 aatcgtcaaa attaccttga aaatccagtg gcacgctact gtaaagaata aaattgccgt
     2341 gtttgatatt acccgtcaaa cgaagcggta aattcagccc tttttcggtt atttctccgt
     2401 ctgaattgga gattacaata ttgcctttac cccaactgtt ttgggaaagc aggctgatac
     2461 gaatcgccgt tttgagtggc aaaaagccgt tttggttgaa tgatttcggt gtgagcttgg
     2521 tgctaacaaa cccttgcagt ggaaattgtt gggtaaagtg ccaatcaaac cgcccttgtt
     2581 gaatttctag gctttcccca tcaaacagaa aagggaaatc cagtttgtta ttttcacttt
     2641 cggcaagttc aacctgccag ttgccttcta acttttgtgc ggcatttttt tgccaactga
     2701 gaatagagcg cccttttgca aggctttatt ggcgatgatt tcataagata attgccaatc
     2761 atattcagcg gaaatagtgg gcggcagttg ggtaaaattg tcctcaattt ctgctgaaat
     2821 cctaagatta tgctgttctt tttcattcgg ttgatatgat aaatcggcat tcaaccgccc
     2881 atttgcaaaa tttgctgcaa atttgaccgc ttgttgagcg gcagttgcct gaatttcacc
     2941 ttttgaaagt gagagtttaa tagctgtttg ctcaaaaagc cgctgaagtc ttgggttgaa
     3001 atcggtaggc aaatttttcc agttaagggc gtggatagtc gcttcgccat caggaatgac
     3061 cgccagcaat gcgttgagtg aagtgtcgga attgctgtgt tcattttgtg gaaacaaggc
     3121 aaggcagcgg taatcaatat ccaatttatc gacagaaaga tggtttttct caatccaacc
     3181 caaatcggtt ttatcggcac ttagtagcgg gcaatctcga taagagagtt gaaattgagc
     3241 aagtgatgag ccttgccaat tcaggtttaa gccttgtggg agttgaactt tccacgcttt
     3301 cggcaaaatc cagttaatcg gcgtggcaag atagtggctg ggcattaaaa aatagtagat
     3361 tgccacaaca gatagaacgg ttgcaatgcc gaaaaatttt aatgcccgtt tcataaatca
     3421 gactacatta gcgaaagggt ataaaatcgc taacagcgta gcaactgtcc aagcggtcgg
     3481 atttttgatg ttttttccaa gtaataacca cgccaacatc aataaaatca caaattgtgc
     3541 tgcaaaatag atgataaatg aaagcaatgg catatccggc ataatttgta gatgttgata
     3601 aagcacaaaa gctgtccagc aataattgag aatcagcacc acaagggcgg ttttcaccaa
     3661 cagtttgagg aaaacatcta aacggctttt cgctaacatt aaataccagc cggctaataa
     3721 aatgcctaac agcacttggg cgaaattgtt ggctcgaatc ccgttaaaca atgaaggcag
     3781 ttcaaaccaa taaatggcta aatattggat aataccaata cttgatatag ccaaaccaca
     3841 atacatcaat gtgttatata aggtttgatc ttccggcagg cggaagtaga ttaagcaggc
     3901 aaaaactgca gcaatgcttg ccaccgccat tgggttatgc tttggcgtaa agcctatgga
     3961 ataaaggaaa ttccctacgg cgaaaataag taaaaagggt aaaataaagc ttaaacggct
     4021 ggcttgccct cggcagattt cgcctttcca tagcactaaa aaactgatta atgaggtcac
     4081 gaaaaacgcc aataagtaag gagaaataaa aattgaattt ttcggtgcag agtagaagtt
     4141 aagagccatt tcaataaaca gcattaatac cattggcagt gcagcgtggg ttgaaatttc
     4201 aagttttgac ggcgaatcgt gatcgtgagc catagataat cccaaaaggt ataaagaaaa
     4261 tgattttaag ccaagattat gccataatag gggtcgtttt ttaacttcag ggggaagaaa
     4321 atatgcgaat tttacataca atgttacgag ttggagattt agagcgctca attaagtttt
     4381 ataccgatgt gttaggtatg cgtctgttac gtagaagcga gaacgagcag tataagtatt
     4441 cgttagcatt tttaggctat gccgatgaaa gcgaaagtgc ggtgattgag ctgacttata
     4501 actggggtgt ggataaatat gagctaggca cggcttatgg ccatattgcg ttaggcgtag
     4561 atgatattta caaaaccatt gaagatgtgc gtgcggcagg cggtaaaatt acccgtgagc
     4621 cgggcccggt attgggtgga acaactgtga ttgcctttgc tgaagatccg gacggttata
     4681 aaatcgaatt tattgagaat aaagatgcga aagcagcgtt aggaaactaa tttttgcgaa
     4741 tatgaatatt caagcggtcg gatttgtaaa aaattttgca aatcagaccg cttgtatttt
     4801 aggttgatag ataaatggaa cagacaacgg aacaaataga ttataaatta ttaaagcatc
     4861 gttttcgtgg ctatttaccg gttgtgattg atgtggaaac agcaggctta aatgcccaaa
     4921 ctgatgcctt attggaattg gcggcaatta ctttaaaaat ggacgagcaa ggttatctag
     4981 tgccggatca acaatgccat tttcatattg cgccatttga aggggcgaat attaaccctg
     5041 agtcattaaa attcaacggc attgatattg acaaccctct aagaggagcg gttgcggaga
     5101 atattgcgat tccggaaatg ttcaaaatgg ttcgcaaagc aatgaaggag caaggctgcc
     5161 aacgggcggt gattgttgcc cacaatgcag cgttcgatca gggcttttta caggctgcga
     5221 ttaaacgaat taatgccaag cgagatccct ttcacccttt cgcgatgttt gacaccgcca
     5281 cccttgccgg ttttatgtat gggcaaaccg tgttggtaaa agcctgccag gtggcaaaaa
     5341 tcccgtttga cggtaagcaa gcgcattctg cgttgtatga taccgagcgc acggcagaac
     5401 ttttctgtgc aatggtcaat cgtttaaaag atttaggtgg tttcccacca ttatcggatt
     5461 aacgttattc atttcattta ttaacacaat ttctcgtatt gtttctataa attatcctgg
     5521 gagtgatgat agttagtggc aagtaccatt ctgcaataaa tccagccctg tagagttgta
     5581 gaaaaaataa aaaactatct atcttcagtt aagattacaa gcggttggtt ttttaaccaa
     5641 atttggcaaa aatgtgacaa aatagaccgc ttgtattcaa taacaataga ctattttttg
     5701 gctataagca ctttctgtat acccgctatt tcgaccctcc ttatttttta tctaatctaa
     5761 aaacaagcgt ttccaccttg aaatgattaa catgatcctg
//
LOCUS       DANLZC010000142         5777 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.142, whole genome shotgun sequence.
ACCESSION   DANLZC010000142 DANLZC010000000
VERSION     DANLZC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.142"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(217..447)
                     /locus_tag="PXE44_002171"
     CDS             complement(217..447)
                     /locus_tag="PXE44_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005720191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589641.1"
                     /translation="MALHRCPECRKRISDSLEHCIHCGFSFKEADLAIYRQKLEQRRL
                     HNQEINRKSVKLQLIWLVIFIAVIALASWITN"
     gene            complement(455..1198)
                     /locus_tag="PXE44_002172"
     CDS             complement(455..1198)
                     /locus_tag="PXE44_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589642.1"
                     /translation="MLNKHINKIINSSFSLAFFTLTSFATAYCYGWGQAIFHGYPWWH
                     VEIGNASMARSLAYVFGTSVILFLCYALGYSLLNKVFQLHYFRNLGWLRVIVLVSVFT
                     VPVILTFYLFTGIVPIYISFIYIFVSGVSVFFFRKRWNNTKFDLDFRKMLLGGSFALF
                     NIFIFLYFSLLSLHIGYLRAGLRTTYDYIEIENQKYYILSTNSDNGYIMGTRIRGNQS
                     FLFFNRDTHQYYHVHIEQCTDSRLCKVIV"
     gene            complement(1369..1707)
                     /locus_tag="PXE44_002173"
     CDS             complement(1369..1707)
                     /locus_tag="PXE44_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5589643.1"
                     /translation="MKLITTIIQPSKLDQVQDALSEIGIQGMTITEVKGFGRQYGHTE
                     QELSQMSRIDFRPKIKVEILVSNYQTDEVIDAILNASYSGQVGDGKIYVANIEQVIRT
                     RTGETDDEAV"
     gene            1966..3864
                     /gene="dnaK"
                     /locus_tag="PXE44_002174"
     CDS             1966..3864
                     /gene="dnaK"
                     /locus_tag="PXE44_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL5589644.1"
                     /translation="MGKIIGIDLGTTNSCVAVMDGDKPRVIENAEGARTTPSIIAYTD
                     KETLVGQPAKRQAITNPKNTLFAIKRLIGRRFTDAEVQRDIEIMPFEISKADNGDAWV
                     TVKGDKLAPPQISAEILKKMKKTAEDFLGEPVTEAVITVPAYFNDAQRQATKDAGRIA
                     GLDVKRIINEPTAAALAYGLDSKKENQTIAVYDLGGGTFDISIIEIDNFDGEQTFEVR
                     ATNGDTHLGGEDFDNRVINYLVEEFQKELGVDLRNDPMAMQRVKEAAEKAKIELSSAQ
                     ETEVNLPYITADATGPKHLNIKVTRAKLESLVEDLVNRSLEPLKTALADAGLSVGDIN
                     DVILVGGQTRMPLVQKKVADFFGKEPRKDVNPDEAVAIGAAVQGGVLSGSVTDVLLLD
                     VTPLSLGIETMGGVMTTLIEKNTTIPTKKSQVFSTAEDNQSAVTIHVLQGERKRAADN
                     KSLGQFNLEGINPAPRGMPQIEVTFDIDANGIINVSAKDKNTGKEQQIKIQASSGLSD
                     AEVEQMVRDAEANAEADKKFEELVQARNQADGIAHATRKQITEAGDALNADDKAKIEA
                     AISELETAAKGEDKAEIEAKIEALIKASEPLMQAAQAKAQGGEQPQQSQKDDGVVDAE
                     FEEVKDNK"
     gene            4006..4308
                     /locus_tag="PXE44_002175"
     CDS             4006..4308
                     /locus_tag="PXE44_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589645.1"
                     /translation="MLKLYPTTENVQPPFYKIYDHKSGETVIATETENSNTAILMDDF
                     VWFEQIPTTEALLADTWSVIENRSKQGLLKRAYTKDFEKHLADGKVEIYIAVNPHC"
     gene            4531..5670
                     /gene="dnaJ"
                     /locus_tag="PXE44_002176"
     CDS             4531..5670
                     /gene="dnaJ"
                     /locus_tag="PXE44_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL5589646.1"
                     /translation="MAKKDYYEVLGLSKGASEKDIKRAYKRLAAKHHPDKNQGSKESE
                     EKFKEITEAYDVLTDSEKKAMYDQYGHAAFEQGGAGAGGFGGFGGGGFGGFEDIFSEM
                     FGGGFGGGGRRQRVVRGDDLRYDIEITLEEAVKGCKKDIRIQTLAECDTCHGTGAEAG
                     SKVETCSHCHGSGRVRRQQGFFVTEAVCPSCHGTGKRIEKPCRSCHGDGRVQKAKNLS
                     VTIPAGVDTGNQLRLSREGAAGENGAPAGDLYVVIHVKEHDIFERDGSNLYCEVPISF
                     TLAALGGEIEVPTLDGKLKLKIPAETQTGKLFRVRGKGVTSPRGGYAGDLICKVVVET
                     PVNLTEEQKALLRQFEESLEGQGKHRPKHEGFFDGVKKFFDNLGK"
BASE COUNT         1881 a         1129 c         1221 g         1546 t
ORIGIN      
        1 cagaaaatta ttaacattct acctattttt taattaacca acatgcaaca aaaaccatat
       61 taacaataag gttatcactg gtttttatcc cttatttttt atgaaaaatc ttataatatt
      121 gaatatttac tccagtttta atcgagaatt atgttctttt ttattttgtt attctcttta
      181 ggtataaaaa aacccggtaa ataccgggct tttatttcaa ttggttatcc aacttgcgag
      241 agcaattacc gcaataaaaa taactaacca aataagttgt aattttacgc ttttgcggtt
      301 gatctcctgg ttgtgtaatc ggcgctgttc taacttttgc cgatatatcg ctaaatcggc
      361 ttctttaaac gagaagccac aatgtatgca atgttctaaa ctgtcgctaa tacgctttcg
      421 acactccgga caacggtgta atgccataat tacctcaaac tattactttg cataatcggc
      481 tatccgtaca ttgttcaata tgaacatggt agtattgatg agtatcacga ttgaaaaaca
      541 agaaagattg attacctctt attcttgttc ccataatgta gccgttatca ctgttggtgg
      601 ataatatgta gtatttttga ttctctattt cgatgtaatc ataggtagtt cttagtccgg
      661 cacgcaaata accaatatgc aatgagagca aactgaaata gagaaaaata aagatgttaa
      721 acagggcaaa acttcctcct aatagcatct ttctaaagtc cagatcaaac ttggtattat
      781 tccatctctt gcggaagaag aatacgctca caccacttac aaaaatatag ataaaagaga
      841 tataaatggg cactatcccg gtaaataagt aaaaagtaag aataacaggc actgtaaaca
      901 cagaaaccaa cacaatcacc cttaaccaac ctagatttcg gaaataatgc agctgaaaaa
      961 ctttatttag taaactataa cctagtgcat aacacaagaa taagatgacg gaagtaccga
     1021 atacataagc caaacttctt gccatactgg cattgccgat ttcaacatgc caccaagggt
     1081 agccatgaaa tatggcttgt ccccaaccgt aacagtaagc ggtagcaaaa ctagttaatg
     1141 taaaaaatgc aagagaaaat gaagaattga taattttatt aatgtgttta tttagcataa
     1201 caaagaatgt ctatttccat taaaacctaa ctatttttga ttactataat aacccgtatt
     1261 acacaaaaaa caaacattta attcacacca aattagaaaa tttgctttag taaggttgaa
     1321 aatataactc taaaacacga taataaaaag cttctctgct agcaatggct agactgcttc
     1381 atcgtcagtt tcgccggtac gggtgcggat aacttgttca atattcgcta cataaatttt
     1441 gccatcaccc acttgcccag aataggatgc attgagaatg gcatcgatca cttcatcggt
     1501 ttggtaatta gaaaccaata tctccacctt aattttagga cgaaagtcaa tgcggctcat
     1561 ttggctaagc tcttgttcgg tatgcccata ttggcgtcca aagcctttta cttcggtaat
     1621 agtcattcct tgaataccga tttctgaaag tgcatcttgc acttggtcta attttgaagg
     1681 ttggataatg gtagtaatca gtttcatatt atatccttct ctgtttgagt tgccgtaagt
     1741 ataaaaagct attgaggggt atgcaaggta ggaggtattt tctccgtgaa atattctgcc
     1801 aaatctcccc cacatacaag cggttacttt ttccaaaaat tttgcaattt ttttattttt
     1861 gcccttgaac agtgcatttt cgtccctatt tatgatttcg tctgaacggg aaatgcaatc
     1921 ccgtttctta ttcaaactga atttattatt ataggaatca taaaaatggg taaaatcatt
     1981 ggtattgact taggtacaac aaattcttgt gttgcggtaa tggacggcga caaacctcgt
     2041 gtaattgaaa atgcggaagg ggcaagaaca acgccatcta tcatcgctta caccgataaa
     2101 gaaaccttag ttggtcagcc ggcaaaacgt caggcaatta ctaacccgaa aaatacttta
     2161 tttgcaatca agcgtttaat cggccgtcgt tttactgatg ctgaagtgca acgtgatatt
     2221 gaaattatgc cgtttgaaat cagcaaagca gataacggcg atgcgtgggt aaccgtaaaa
     2281 ggcgataaat tagcaccgcc gcaaatctct gctgaaatct tgaaaaaaat gaagaaaacc
     2341 gcagaagatt tcttgggcga accggtaacc gaagcggtta ttactgtgcc tgcatacttt
     2401 aacgatgcac aacgtcaggc aactaaagat gccggtcgta ttgcaggttt agatgtaaaa
     2461 cgtatcatca acgaaccaac agcggcagcg cttgcttatg gtttagattc taagaaagag
     2521 aaccaaacga ttgctgttta cgacttaggt ggtggtacat tcgatatttc gattatcgaa
     2581 attgataact ttgacggcga gcaaactttt gaagtgcgtg caaccaacgg cgatactcac
     2641 ctaggtggtg aagacttcga taaccgtgta atcaactact tagtggaaga attccaaaaa
     2701 gaactaggcg ttgatttacg caacgaccca atggcaatgc aacgtgtgaa agaagcagcg
     2761 gaaaaagcga aaatcgaact ttcttctgca caagaaactg aagtgaacct tccatacatc
     2821 acggcagatg caaccggccc gaaacactta aacattaaag taacccgtgc gaaattggaa
     2881 tctttagttg aagatttagt aaaccgttcg ttagagccgt taaaaaccgc tttagctgat
     2941 gccggtttat cagtaggcga catcaacgat gtgattttag tcggtggtca aacccgtatg
     3001 ccgttagtac agaaaaaagt ggcagacttc tttggcaaag aaccgcgtaa agacgttaac
     3061 ccggacgaag ctgtggcaat cggtgcggcg gttcaaggcg gtgtgttaag tggttcggta
     3121 acagacgtat tgttattaga cgttactccg ctttcacttg gtatcgaaac aatgggtggt
     3181 gtaatgacta ccttaattga gaaaaacact acgattccaa ccaagaaatc acaagtgttc
     3241 tcaacagcgg aagataacca atctgcggta actatccacg tgttacaagg tgagcgtaaa
     3301 cgtgcagcgg ataataaatc tttaggtcaa ttcaaccttg aaggtattaa ccctgctcca
     3361 cgcggtatgc cacaaattga agtaaccttt gatattgatg ctaacggtat tatcaatgtc
     3421 tctgcaaaag ataaaaacac tggcaaagag caacaaatca aaattcaagc atcttcaggc
     3481 ttaagcgatg ctgaagttga acaaatggtg cgtgatgcag aagcgaatgc ggaagcagac
     3541 aaaaaatttg aagagttagt tcaagctcgt aaccaagcag acggtattgc tcacgcaacc
     3601 cgtaagcaaa ttaccgaagc aggcgatgcg ttaaacgcag acgataaagc gaagatcgaa
     3661 gcggctattt ctgagttaga aaccgcagct aaaggcgaag ataaagccga gatcgaagcg
     3721 aaaatcgaag cgttaatcaa agcttcagaa ccgttaatgc aagcagcaca agcaaaagca
     3781 caaggcggcg agcaaccaca acaatctcaa aaagatgacg gcgtggtcga tgcggaattt
     3841 gaagaagtga aagataataa ataatcttgc gtaacttgta ggggcgaaat atctttcgcc
     3901 ccttttgtgt agatatatac cacctagcac gagccgtgct aggttacaca taattcagcc
     3961 cctttggggc tgtgcaagcg gtgcttttca aaggaaattt tacaaatgct aaaactctac
     4021 cctacaacgg aaaatgtaca gccaccgttt tataaaatat acgatcataa aagtggcgaa
     4081 acggtcattg ccacagaaac ggaaaacagc aataccgcga ttttaatgga cgattttgtt
     4141 tggtttgagc aaattccaac taccgaagct cttttggcag acacttggtc tgtgattgaa
     4201 aaccgttcca aacaaggttt gctcaaacga gcttacacca aagattttga aaaacattta
     4261 gcagacggca aagtagaaat ttatattgcg gtgaatccgc attgttaggc ttttgatagc
     4321 gcaagcgtcc acgcttgtgt catcttgagc catcttggct agatcccatt taattatata
     4381 aaactctctc cccttgtggg agagagaccg aaaaatattt cgttcagaaa tatttttcag
     4441 agagaggggg aattaaccat cctacccctc tcccacaagg ggcgagggca agcggtcaaa
     4501 aacacagaaa aatttacaaa cggaacaaca atggcaaaga aagattatta cgaagtcctt
     4561 ggactgtcta aaggggcaag cgagaaagac atcaaacgag catataaacg tttggcggca
     4621 aagcatcacc cggataaaaa ccaaggcagt aaagagtcgg aagagaaatt taaagagatt
     4681 accgaagcct atgacgtgct gaccgacagc gagaaaaaag cgatgtacga ccaatacggt
     4741 cacgccgcct ttgaacaagg cggtgccggt gcaggcggat tcggtggctt tggcggcggt
     4801 ggtttcggtg gttttgaaga catcttcagc gaaatgtttg gcggtggctt tggtggcggt
     4861 ggacgtcgcc aacgtgtggt acgtggcgat gatttacgtt acgacatcga gatcaccctt
     4921 gaagaagctg tgaaaggctg taaaaaagat attcgcattc aaaccttagc tgaatgtgat
     4981 acttgtcatg gtacaggggc ggaagcaggg agcaaagtgg aaacctgttc gcattgtcac
     5041 ggctccggca gagttcgccg tcagcaaggt ttctttgtga ctgaggcagt ttgtccaagt
     5101 tgtcacggta caggtaaacg cattgaaaaa ccgtgccgtt cttgccacgg cgatggacgt
     5161 gtacagaaag cgaaaaacct ctctgttacc attccggcag gtgtggatac cggtaatcaa
     5221 cttcgcttat cgagagaggg agctgcaggc gaaaatggcg caccggcagg cgatttatat
     5281 gtggtgattc acgttaaaga acacgatatt tttgagcgtg acggttcgaa tttatactgt
     5341 gaagtgccga ttagctttac ccttgcagct ttaggcggtg aaattgaagt gccaacttta
     5401 gacggcaaat taaaactcaa aattccggca gaaactcaaa ccggtaaact attccgtgta
     5461 cgaggcaaag gggtaacaag ccctcgtggt ggctacgcag gggatttaat ctgcaaagtg
     5521 gtggtcgaaa cgcctgtgaa tttaaccgaa gaacagaaag cactattacg acaatttgaa
     5581 gagagtttag aaggacaagg taaacatcgt ccgaaacacg aaggtttctt tgatggtgtg
     5641 aagaaattct ttgataactt aggcaaataa aatcataaaa agtacatatt tcttaaatat
     5701 gtactttttt attatctatt ctttgttgaa atctacttct cggccgttaa tttttacacc
     5761 tacatactgc aaaaaat
//
LOCUS       DANLZC010000143         5742 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.143, whole genome shotgun sequence.
ACCESSION   DANLZC010000143 DANLZC010000000
VERSION     DANLZC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.143"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..153
                     /locus_tag="PXE44_002177"
     CDS             <1..153
                     /locus_tag="PXE44_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5589647.1"
                     /translation="QMVIHHSPNRLSKRDLYDGYWLRHTHSIWRHKLADKLDFDGILN
                     DIAVNN"
     gene            422..1051
                     /locus_tag="PXE44_002178"
     CDS             422..1051
                     /locus_tag="PXE44_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL5589648.1"
                     /translation="MITVKFYGHLKTFGTDFKILVKDIAEAIRALCSQLKGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVVKGAKSGGIFQAVLGVALIGAA
                     FALGPIGLGLVGSATAMTVGAMGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
     gene            1061..4792
                     /locus_tag="PXE44_002179"
     CDS             1061..4792
                     /locus_tag="PXE44_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDL5589649.1"
                     /translation="MRIYGAKGGGGSSHTPIEAPETGRSKQIVNIVELLCEGEIEGLV
                     DGFKSIYLDGTKIQNDDGTYNFNNVSGQLNVGTQDQDVLDGYDSSQNEVNVGVEIKKK
                     NGAIVRTVTDERINRLRLTLGVRSLFHQNNQGDTNTTNVDLKITIGTRQYSHSFNGKY
                     SSQYLESVVFDNLPPVPFNISVERVTEDSNSQRLQNGTIWSSYTEIIDTEFTYPNSAV
                     AGISFDSEYFNNIPTRNYLIKAKKVKVPSNYDPVKRTYTGFWDGTFKVAWTNNPAWEI
                     YDLAPILSKMLGVEISFDKWALYDVARYCDQLVPDGMGGMEPRFTCNVWLTEVKTAYD
                     LLNDFCSVFRAIPIWTGTEVSVIIDRPRDPVWTYTNANVVGGFERSYSARKSRHNAVQ
                     VTYSDKTNGYESAIEYVSDDEEIKKHGLNLSQITAFGCTSRGQAHRTGKWILETEKRE
                     KETITFTVGREGLMHLPGDIIRVADSHYAGTEIGGRVLAINGRKVTLDREISIDNASY
                     FTYINGEATHSSKIQSVNGKEITLDSTPTGLETYGVWSLSTKQISSGLYRSISIVENA
                     DGTNTITALQHEPQKEAIVDNSAHFVETARTLYKAPQINAVEVSTGYDGKLYITGDIS
                     SGDGKLTYDIKITKDGNLYQYKKGLSDPNIELSDLPNGDYTVIIYGKNAKGQIVTEKT
                     QTFTIDRPPAPTGVVVTGGLGQITLEWDWVNEVTQTEIFAAETDNFALAKKIAKVTAR
                     TYAHTLKGNKVVRYYWLRHTRGINVGPFYQQQGVRGQTAVDLDARLTELNTQLSRNIV
                     NEVFDVAAPARGLELVKTVANLTDKGTKLASSQVYNQADGKLYTWNGTAYSATVAAED
                     VTGKLSKSKIDTSLISQLTGADNTANLARRLAETAQSNINQEITNRQDAVTAEANNRT
                     KAIQAESANLTKKIQAEATARGAAVTQLQNVDAQQAQLISAVTAKADNALSGLEEEKT
                     ARANGDKAESQAREALTARMGAAESNIVTIQRTVANNAQSISEVSQNLNAKIDNINIG
                     GRNLLRDSEFNAYNKWGSPQIEFAENANRRTIKVTSTGTNGPVGIVSSNRHSTSYFQQ
                     GETYTLSLFARGSKALDYLYLMRQDGNNVRLPVINVASETEFNHYKLTFKAPFTTQQG
                     YVLIGFRQTSTEQFVEFHSVKLEKGNVATDWTPAPEDVESAVSAVSADLTSYKQTQAA
                     TDSAQAQQLNQLSVNLTKAETNFNAKITEEKKLVLMQIK"
     gene            4789..>5742
                     /locus_tag="PXE44_002180"
     CDS             4789..>5742
                     /locus_tag="PXE44_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5589650.1"
                     /translation="MNADKLTDITSRVANAESTITNFQSTKANKSEVASIAQQNLQSI
                     WRTDAQSAVDALKIGGANLLVDSEYLTTARWGGSSRVASSQYGDRRLTQVFVTQAGTG
                     HFGVTQGTQKATTRIRQGETYTLSLNAQGTAGFTRTGLNYVYLIREDGGNFRLPTLPL
                     TASLSQRPKVTFTAPWTSNQVRLLIGANGIFEATDWFAFHSVKLEMGNVATGWTPTAK
                     DIDDKVSAVQSNLTAYQAAQAKADQAKATQISGLTTRMGAAESNLTRTERAVTELNQT
                     TVTTLRDLTARTKTTEGSLSRLETAKASKTEVASIAQSSLQS"
BASE COUNT         1806 a         1198 c         1313 g         1425 t
ORIGIN      
        1 cagatggtaa tccatcatag cccgaaccga ttatctaagc gtgatttata cgacggctat
       61 tggttacgtc atacccacag catttggcgg cataaactgg cggataagtt agattttgac
      121 ggcatattga atgatattgc tgtaaataac taacaaaaaa tgaccgcttg taacaaaaac
      181 aaaccccgaa gcgtttgcag cacttcgggg tttttcatat ccacttacct aactaagagg
      241 acataaattg aatgaatgat aaacgattta cattcaaatt tctaggagtt cttatggaag
      301 caataaatat taccccaaaa gaaatcagaa aaacaatgtg gacagccgca ataattctct
      361 ttttgtttgc gttcatctgg aaagcccctg aatttatcac tgctattcgt tggtggtaac
      421 tatgattaca gttaaatttt acggacacct taaaacattc ggcacagatt ttaaaatttt
      481 agttaaagac atcgctgagg caattcgtgc tctgtgttca cagcttaaag gattgcgtga
      541 agcgttgcga gacggtgttt ataaagtccg tatcggtaag cagtatttag acccatcagc
      601 ccttgaaaaa gggctttttt attgcctgaa gaaagggcaa accattcatt ttacccctgt
      661 tgtcaaagga gcgaaaagcg gtggtatttt tcaagcggtg ttaggtgtgg cgttaattgg
      721 ggctgcattt gcgttaggtc ctatcggctt aggattggtt ggctctgcta cagcaatgac
      781 agttggagca atgggggcat caatgttact aggaggagtt tcccaaatgc tcaccaaaat
      841 gccaaaagcc cccacaatgg gtaatgaaac agaaaaagaa agctcaacgg ctttttcaaa
      901 tttaaataac ttggtggctc aaggcaaacc tgtgccgttg gcttatggtt tgattcgtac
      961 cggctcgctg gtgatatcgc agggagttga gacgatcacc attaaagaaa accaaccggc
     1021 aagcaacaag aaaaccggct ttagaaaata agggataatt atgcgtattt acggagcaaa
     1081 aggtggtggc ggcagtagcc ataccccgat tgaagcaccg gaaaccggtc gctcaaaaca
     1141 gattgtgaat attgtggaat tgctgtgcga gggcgaaatt gaaggcttgg tagatggctt
     1201 taaatcaatt tatcttgacg gcacgaaaat ccaaaacgat gacggcacct ataattttaa
     1261 taatgtgagc ggtcagctaa atgtcggtac gcaagatcaa gatgtgttgg acggttacga
     1321 tagctcacag aatgaagtga atgtgggtgt tgagattaag aaaaagaatg gggctattgt
     1381 ccgcaccgta actgatgaac gaattaaccg cttgcgttta acgttgggag taaggtcatt
     1441 attccaccaa aataatcaag gcgataccaa taccaccaat gttgatttaa aaatcacgat
     1501 tggcacacgg caatattcgc atagctttaa cggcaaatac agctcgcaat accttgaatc
     1561 tgtggtgttt gataacctgc cgcctgtgcc gtttaatatc tcggttgaac gtgtaacaga
     1621 ggatagcaat tctcaacgac tacaaaacgg tacaatttgg agcagctata ccgaaattat
     1681 cgacaccgaa tttacttacc ctaattcagc ggttgccggt atcagttttg attcggagta
     1741 ttttaataat atcccgaccc gaaactacct aattaaagcg aaaaaagtca aagtgccaag
     1801 caattatgat ccagttaaac gcacttacac cggcttttgg gacggcacat ttaaagttgc
     1861 gtggacgaac aaccctgctt gggaaattta cgatttagct ccgattttaa gcaaaatgct
     1921 tggggttgaa atcagttttg acaaatgggc gttgtatgat gttgctcgct attgcgatca
     1981 acttgtgccg gacggtatgg gtggaatgga gcctcgtttc acctgtaacg tatggctcac
     2041 ggaagttaaa actgcctatg acttgttgaa tgatttctgc tcggtattcc gtgcgattcc
     2101 gatttggacc ggaacagaag tatcggtcat tatcgaccgt ccgcgtgatc cggtttggac
     2161 ttacacaaat gctaatgtag ttggtggatt tgaacgatcc tactctgctc gcaaatcacg
     2221 ccataatgca gtgcaagtta cttattcgga taaaacaaat ggctatgaaa gtgcgattga
     2281 atatgtctct gatgacgagg aaatcaaaaa gcacggctta aatttaagtc aaattactgc
     2341 tttcggttgt acctctcggg ggcaagcaca ccgtaccgga aaatggattt tggaaaccga
     2401 aaaacgggaa aaggaaacca ttacttttac cgtgggacgt gagggcttaa tgcacttgcc
     2461 gggcgatatt attcgtgtgg cagacagcca ttatgccggt acggaaattg gcggacgtgt
     2521 actggcgatt aatgggcgaa aagtcacgtt agatcgtgaa attagcattg ataacgccag
     2581 ttattttacc tatatcaacg gtgaggcaac gcactctagc aaaatccaat cggtaaacgg
     2641 caaagaaatt acgctggata gtacaccaac cggcttagaa acctacggtg tttggtcgtt
     2701 atcgactaaa caaatcagct ctggattata tcgctcaatc tcgattgtcg aaaatgcgga
     2761 cggcacaaac actatcacag cgttacaaca cgaaccgcag aaagaggcaa ttgttgataa
     2821 ctccgctcac tttgtggaaa cggctagaac actctacaaa gctccacaaa ttaacgcagt
     2881 ggaagtctcg accggttatg acggtaagct ctacattacc ggtgatatta gctccggtga
     2941 tggcaagcta acctatgata ttaaaattac caaagatggc aatttatatc aatacaaaaa
     3001 aggcttaagc gatccgaaca ttgaactaag tgatttacct aacggcgatt ataccgtcat
     3061 tatttacggc aaaaacgcca aagggcaaat cgtcaccgaa aaaacacaaa cctttactat
     3121 tgatcgacct ccagctccga ccggtgtagt agtaacaggt ggattagggc aaattaccct
     3181 cgaatgggat tgggtaaatg aggttacgca aaccgagatt ttcgcggccg aaaccgataa
     3241 tttcgccctt gcaaaaaaaa tcgcaaaagt gaccgctaga acctacgctc acacactaaa
     3301 aggtaacaag gttgtacgct attattggct acgtcatacc agaggcatta atgtcggacc
     3361 gttttatcaa caacaggggg taagaggaca aacagcggtt gatttggatg ctcgattaac
     3421 cgaattaaac actcagttat cacgcaatat tgttaatgag gtttttgatg ttgctgcacc
     3481 ggcaagaggt ttagaattag tgaaaacagt cgctaatttg accgacaaag gtactaaact
     3541 ggcgtcatct caagtttaca atcaggctga cggcaagctc tatacttgga acggtacagc
     3601 atactcagct acagtggctg cggaagatgt taccggcaaa ttatcaaaat ctaaaattga
     3661 tacgtccctt atttcgcaat taacaggtgc agataacacc gcaaatttgg caagaagatt
     3721 agcggaaaca gctcaaagca acattaacca agagattact aaccgacaag atgccgttac
     3781 tgccgaagca aataaccgta ctaaagccat tcaagccgaa agtgcgaatt taacgaaaaa
     3841 aattcaagct gaagcaaccg ctcgtggggc tgctgttaca cagttacaaa atgttgatgc
     3901 tcaacaagca cagttgattt ctgctgtcac agcaaaagct gataatgctc tttcaggctt
     3961 ggaagaggaa aaaacagcac gagcaaacgg cgataaagcg gagtctcaag cacgtgaagc
     4021 attaacagca agaatgggag cagctgaaag caatatcgta actattcaac gtacggtagc
     4081 aaataatgcc caaagcatta gtgaagtcag tcagaattta aatgctaaga ttgataatat
     4141 taatatcggc ggtcggaatt tattaagaga cagcgagttc aatgcgtaca ataaatgggg
     4201 tagtccacaa attgaatttg ctgaaaatgc aaatcgccga actatcaaag taacctcaac
     4261 aggtactaac ggtcctgttg gtattgtttc atctaatcgc cattccacat cttatttcca
     4321 acaaggagaa acttatacac tctcattatt tgctagagga agtaaggcac tagactatct
     4381 ctatctaatg cgtcaagatg ggaataacgt tagattacca gtgattaacg ttgcctctga
     4441 aactgagttt aatcattaca aactcacgtt caaggctcct tttaccacgc aacaaggtta
     4501 tgtcttaatc ggttttcggc aaaccagcac agagcagttt gttgagtttc acagcgtcaa
     4561 acttgaaaaa ggcaacgtag ccacagactg gacaccagca ccggaggatg tggagagtgc
     4621 ggttagtgct gtttcggctg atttaacgag ttataaacaa actcaagcgg ctaccgattc
     4681 agcacaagca cagcaactta atcagttatc tgtgaacttg accaaagcag agactaattt
     4741 caacgccaaa atcacggaag aaaaaaagct cgtgttgatg cagataaagt gaatgcagat
     4801 aaactcacag acataacaag ccgtgttgca aatgcggaat caactattac caatttccaa
     4861 tcaaccaaag cgaataaaag tgaggtggca agtattgccc agcagaactt acagagcatt
     4921 tggcgcaccg atgcgcaaag tgcggtggat gccttgaaaa tcggtggtgc aaatctactg
     4981 gttgatagtg agtatttgac gacagcccgt tggggcggta gctcacgagt ggcaagctcg
     5041 caatatggtg atcgtcgctt gactcaagtg tttgtgaccc aagcaggcac agggcatttt
     5101 ggtgtgactc aaggcacaca aaaagccact acgcgtattc gtcagggtga aacctatacc
     5161 ttgtcactaa atgcgcaggg gacggcaggt tttacccgca cagggttaaa ctatgtgtac
     5221 cttatccgcg aagatggtgg caattttaga ttgccaactt tgccgcttac tgcatcattg
     5281 tcccaacgcc ctaaagtgac atttactgcg ccgtggacga gcaatcaagt tcggttactg
     5341 attggcgcaa atggcatatt tgaggcgaca gactggttcg catttcacag cgttaagctc
     5401 gaaatgggta atgttgccac aggctggaca ccaaccgcca aggatattga cgacaaagta
     5461 tcagcggttc agtccaacct caccgcttat caggcggcac aagctaaagc agatcaagcc
     5521 aaagctacgc agatttcggg gctcacaacc cgaatggggg cagcggaaag taacctcact
     5581 cgcaccgaac gggcagtgac ggaacttaat cagacaacgg tgacgacact gcgtgatcta
     5641 accgcgcgca ccaaaacaac ggaaggttcg ctctctcgtt tggaaaccgc caaagcaagt
     5701 aaaactgagg tggcaagtat tgctcaaagc tccttacagt ca
//
LOCUS       DANLZC010000144         5691 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.144, whole genome shotgun sequence.
ACCESSION   DANLZC010000144 DANLZC010000000
VERSION     DANLZC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.144"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            379..1383
                     /locus_tag="PXE44_002181"
     CDS             379..1383
                     /locus_tag="PXE44_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL5589651.1"
                     /translation="MPGDANKIISNGTSAGGALSTLLGASADHYDYEPYLKEAGALNA
                     SDKIFAVSAYCPITNLENADMAYEWQFNGVNEYSRIDMSRLNAAEFNDRSKPKPKPKI
                     EGSLNEAEIKVSNELAERFPTYLNSLHLVDEKGNPLTLDPKGNGSFKDYLSEVVKTAA
                     NKAYRGLVQDSEEQKAFQQISWLSFEKGKVSSVDWFGYVFSDKRMKSPPAFDALNGSS
                     GENNLFGTDTENNRHFTLYSAERSANKDLNLADPQIVKRMNPMHYLDNPNAAEHWRIR
                     VGTADRDTSLAISAILAIKLQMAGKNVNYETPWNVPHSGDYDVNELFLWADELVKGKK
                     "
     gene            1438..3288
                     /locus_tag="PXE44_002182"
     CDS             1438..3288
                     /locus_tag="PXE44_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5589652.1"
                     /translation="MQVKKFSQKYINWVLKLGRLKASLLGFFMVALSAILIQSSLTYL
                     FIGRVVVVDILRSIAFGLISAPFVLYFFTLLVEKLEISRLKLEKAVRDLEELREQDAY
                     LNAKLEKNSRDKSILMATISHELRTPLNGIIGLSQILLDDKTTEKQQEYLKTINMSAT
                     ALAHIFGDIIDLEKIDSSRIELVRKEVEFEQLIHDIANFGHFMAGNKQINFEVKYPPD
                     LPKYIYIDHARLSQILWNLISNAVKFTPASGRILLEIYQLDKEQFSFAVTDSGIGIPE
                     KDQRKIFDMFYQAENSKEKKAQGSGIGLSVSKRIAKLMGGDLVVASQENQGSTFTLTV
                     QAESTETKPIFAIQQHSLKVLLVEDIEMNVVVARAMLEKIGCVVDVAMSGEETFQHFA
                     QNSYDLILLDIQLPDTTGFEIAQQLRLQYENEEIDYLPLLVALTANVIQSRQEYQAQG
                     MDDVLHKPLSLDELMRCLNQHFGDDFLQKNEEKQPLVERGSENLPYSITILNELIGVM
                     GKENVLANMDLFADLMPTYLANLQRYLSDWQTTNSVESRKLTADEAHKIKGALASVGL
                     HQLQQTAQLAQSDNGEQWEQNIAHWVEMICSKWQMDLQLAKEYVRSKGDI"
     gene            complement(3384..3938)
                     /locus_tag="PXE44_002183"
     CDS             complement(3384..3938)
                     /locus_tag="PXE44_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL5589653.1"
                     /translation="MKSQIYYDYYPSPIGQLLLLATEEGLIYIELGQEQQTTSLDNFL
                     SAQAASGQILEIFCKTKEILDRYFAGEPLDFQQLDFLAPQGTPFQQSVWKILREIPYG
                     QTTTYGEIATQLGKPSAMRAVGGAVGRNPISILVPCHRVLGKNQALTGFGGGLPNKRF
                     LLQLEDITYKDKGIEFVKPKTKKW"
     gene            complement(4005..5486)
                     /locus_tag="PXE44_002184"
     CDS             complement(4005..5486)
                     /locus_tag="PXE44_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HDL5589654.1"
                     /translation="MKMKNFSKTLIASLVAFSSAGASAAAFQLAEISTSGLGRAYAGN
                     AAVADNASVVATNPALMTLFKQTEISAGGIFVDADVNIDGKLNATGADASHKNVIPTA
                     IVPNAYIVSPINERLSIGGGVNVNYGLISEFDPNYSAGFLGGRTSLSAVNVNFSAAYD
                     LGYGFVLGAGLNAVHAKAELGRFLGAGAARFAPAKQFLEDKKVQVQSAIQGLKALEAG
                     IAAGNTALEAQKTQVLAQVNGLLKSQNLNQTITNSAQLERLVNGIQNTNASSTIHKLK
                     GDKWALGWNVGLVYNINERNRLGFTYHSEIDLKFKGKYSNTVPVVIPKMVDTITGGAE
                     IDGTLRLRLPAFWEVSGYHKLTDKLAAQYSYKRTDWSKFQSLEAYDMQGNRLLHKTEN
                     FNDSQRIALGLSYDVNEMLTLRTGVAFDESASVTHPSISIPDTDRAWYSVGATVRFTP
                     NLSADVGYSHLRGSNLTFSEDGLATFKSKARANLYGLNVNYKF"
BASE COUNT         1790 a         1018 c         1208 g         1675 t
ORIGIN      
        1 gcaaaaatga accaggcaga agcgatgaga tgatcgactt catcttttga taaactcttt
       61 gcgatttaaa cagaaaatat atttagaatg taaacaaaca tgagtcgatt ttatgttcaa
      121 agagctgtaa taagacggaa taatgtcaga ccaagataga tactttcaag taaaatcaag
      181 cataaaaatg attttcagac tttacaaatc tgaaagaatg tgtaagatca ggattgtccg
      241 aaattcggat ggaaaatccc agtggatgta ttggctggat acatcataat aactatgtat
      301 ttgtgaatgt tatttgaatg attctacgta gttgattcaa aatcagcagt gcggtatctg
      361 catgctaatg atgaaaaaat gccgggcgat gcaaataaaa ttatctctaa cggcaccagt
      421 gccggaggag cattatctac tttgttagga gcttcagctg atcattacga ctatgagcct
      481 tatttgaaag aggcgggggc attgaatgcc agtgacaaaa tttttgcagt ttctgcctat
      541 tgcccaatta ccaaccttga aaatgcggat atggcgtatg aatggcaatt taatggtgtc
      601 aatgaatata gccgaatcga tatgagtcga ttaaatgcag cagagtttaa cgaccgctca
      661 aaaccaaaac caaagccgaa aattgaaggc tcgttaaatg aggcggaaat taaagtttct
      721 aatgaattag cagaacgctt tccgacttat ttgaacagtt tgcatttggt tgatgagaaa
      781 ggcaatcctt taacacttga tccaaaaggc aacggctcgt ttaaagatta tttatctgaa
      841 gtggtaaaaa cggctgcaaa taaagcctat agaggtttag ttcaagatag cgaagagcaa
      901 aaagcctttc aacaaattag ttggctttca tttgagaaag gcaaagtcag cagtgtggac
      961 tggtttggtt atgtgttttc agataaacgg atgaaatcac cacctgcatt tgatgcgtta
     1021 aatggaagtt cgggcgagaa taatttattt ggcacagaca cggaaaataa ccgccatttt
     1081 acgctttata gtgcggaacg ttctgccaat aaagatctca atttagccga tccgcaaatc
     1141 gtgaaacgga tgaacccgat gcactatttg gataacccaa atgctgcgga acattggcgg
     1201 attagggtgg gaacggcaga tagagatacc tcgctggcaa tttctgcaat cttagcaata
     1261 aaactgcaaa tggcaggcaa aaatgttaat tatgaaaccc cgtggaatgt accccattca
     1321 ggtgattatg atgtaaatga attattccta tgggctgatg agctagtcaa aggcaaaaag
     1381 taaaaaatag agtaaaataa agcagatctg atggtctgct ttttgttttg aggtcgtatg
     1441 caagttaaaa aatttagcca aaaatatatt aattgggtat taaagctagg gcggttaaaa
     1501 gcttctcttt taggcttttt tatggtcgct ctttcggcaa ttttaattca aagtagctta
     1561 acctatcttt ttattgggcg agtagtcgtg gtggatattt tacgctccat cgcttttggt
     1621 ttaatttctg cgccttttgt gctttatttc ttcacgttat tggtggaaaa acttgaaata
     1681 tctcgcttga aattagaaaa agcagtaaga gatttggaag aactccggga acaagatgcc
     1741 tatcttaacg ctaaattaga aaaaaacagc cgtgataaaa gcattttaat ggcgaccatt
     1801 agtcacgaac ttcgtactcc ccttaatgga attatcggct tgagccaaat tttgttagat
     1861 gataaaacca ccgaaaaaca gcaagaatat ctaaaaacta tcaatatgag tgccactgca
     1921 ttggctcata tttttggcga tattattgat ttagaaaaaa ttgatagtag ccgaattgaa
     1981 ttggtaagaa aagaggtgga atttgaacaa ctcattcacg atattgctaa ttttgggcat
     2041 tttatggcgg gcaataagca gattaatttt gaagtgaagt atccgcctga tttgccgaag
     2101 tatatctata tcgaccacgc acgcttgagc caaattttat ggaatttgat cagcaatgcg
     2161 gtgaaattta cccctgcaag cggtcgaatt ttgctggaaa tttaccaatt agataaagaa
     2221 cagtttagct ttgcggtgac ggattcaggc atcggtattc cggaaaaaga tcaacgcaaa
     2281 atttttgata tgttctacca agcggaaaac tctaaagaga aaaaagcgca aggtagcggg
     2341 attggcttat cggtctcgaa acgcattgcg aagctaatgg gcggtgattt ggtggtggca
     2401 agccaagaaa atcaaggctc aacttttacc ctcactgtgc aggcagaaag cacggaaacc
     2461 aagccgatat ttgctattca gcaacattcc cttaaggtgc tattggtaga agacattgaa
     2521 atgaatgtgg tggttgcccg agcaatgttg gagaaaatcg gttgcgtggt tgatgtagca
     2581 atgagtggtg aagaaacgtt ccaacatttt gcacagaaca gctacgatct gattttactt
     2641 gatattcaac tgccggatac caccggtttt gaaattgcac agcaattaag attgcaatat
     2701 gaaaacgagg aaattgacta tttaccgctg ttggttgctt tgacggcaaa tgtgatccaa
     2761 agccggcagg agtaccaagc acaaggtatg gacgatgtgc tacacaaacc gctctcttta
     2821 gatgaattaa tgcgttgtct caaccaacat tttggagatg attttttgca aaaaaatgaa
     2881 gaaaaacagc cgcttgtaga gagaggttct gagaatttac cttatagcat tacaattctt
     2941 aatgaattga ttggggtaat ggggaaagag aatgtgcttg ccaatatgga tttatttgct
     3001 gatttaatgc ccacttattt ggcaaattta cagcgttatt taagtgattg gcaaaccaca
     3061 aattctgtgg aaagccgtaa attaacggcg gacgaggctc ataagattaa gggagcatta
     3121 gcatcagtcg gattacatca gctacaacaa actgctcagc ttgctcaatc cgataacggc
     3181 gagcagtggg agcaaaatat tgcacactgg gtggaaatga tttgttccaa atggcagatg
     3241 gatttgcaat tagctaagga atatgttaga agcaaggggg acatttagta aatttgaaat
     3301 gttacgatgg tgcaagcgtc cacgcttgta ccaattttaa tatctgaagc acaagcgtgg
     3361 acgcttgtgc tatcaaaacg tatttaccat ttcttggttt tcggttttac aaattcaatg
     3421 cctttatctt tataggtaat atcttccagc tgtaatagaa aacgcttatt cggcaagcca
     3481 ccaccgaatc cggttaaggc ttgatttttc cctaataccc tatggcaagg cactaaaatc
     3541 gaaatcgggt ttcgcccaac tgcaccgccg acggctcgca tcgcacttgg cttgcctagt
     3601 tgggtggcaa tttcgccata agtagtagtt tgcccgtaag ggatttcacg caaaattttc
     3661 caaactgatt gctggaatgg agtgccttgc ggggcaagaa aatccaactg ctgaaaatcc
     3721 aatggctcgc cggcaaaata gcggtctaaa atttctttgg ttttgcaaaa aatttctaaa
     3781 atctgaccac ttgcagcttg agcggaaagg aaattatcaa gcgaggtggt ttgttgctct
     3841 tgccctagct caatgtaaat caagccttct tctgtggcta aaagcaataa ttgcccgata
     3901 gggcttgggt agtaatcgta gtaaatttga gatttcatgt ttttatgcca ataaaaaaag
     3961 aggtgtaatt gattttacac ctctttaaac cttaattcat caaattagaa tttgtagttt
     4021 acgtttaaac cgtaaagatt tgcacgagct ttgctcttga atgtggctaa accgtcttca
     4081 gagaaggtca ggttgctacc acgaagatgt gagtaaccta catcagcaga taggtttggc
     4141 gtgaaacgca cggttgcacc tacagaatac caagcacgat cagtgtccgg aattgagatt
     4201 gatggatgag ttacacttgc actttcatca aatgctacac ctgtacgcaa tgttagcatt
     4261 tcattcacat cataagacaa gcctaatgca atacgttgag agtcattaaa gttctcggtt
     4321 ttgtgcaata ggcggttacc ttgcatatca taagcttcta ggctttggaa tttgctccag
     4381 tctgtgcgtt tatagctgta ttgtgcagca agtttatcgg ttagtttgtg gtaacctgac
     4441 acttcccaga atgccggtag gcgtaatctt agtgtaccat caatctctgc accgccggta
     4501 atggtatcta ccatcttagg aataactaca ggaacggtat tagaatattt gcctttaaat
     4561 ttaagatcaa tttcagagtg gtaagtaaaa cctaaacggt ttctttcatt gatattataa
     4621 accaagccaa cattccaacc caatgcccat ttatcgcctt taagtttatg aattgttgaa
     4681 cttgcattgg tattttgaat gccattaacc aaacgttcta attgagcaga attcgtgatg
     4741 gtttggttta aattttgaga ttttaataaa ccgtttactt gagctaatac ttgtgttttt
     4801 tgagcttcaa gagcggtatt acctgctgcg atacctgcct caagagcttt taaaccttga
     4861 attgcacttt gtacttgtac tttcttgtct tccaaaaatt gtttagctgg agcaaaacgt
     4921 gccgcaccgg cacctaagaa acgtcctaat tctgcttttg cgtgtactgc attcaaacct
     4981 gcccctaata caaaaccata acctaaatca taagccgcac taaaattaac attcacagca
     5041 cttaatgatg tacgaccacc taagaaacca gcagaatagt tagggtcaaa ttcagatatc
     5101 aaaccataat ttacatttac gccaccacca atagaaaggc gctcattgat aggtgaaacg
     5161 atataagcat ttggaacaat agcggtagga atgacatttt tatgtgaggc atctgcacct
     5221 gtagcattga gctttccatc aatatttaca tcagcatcta cgaaaatacc accggcagaa
     5281 atttcggttt gtttaaataa cgtcattaac gcagggttgg ttgcaacaac agaagcgtta
     5341 tcggctacag cggcattacc tgcgtaagct ctacctaaac cggatgttga aatttcagct
     5401 aattggaatg cggcagcaga tgcaccggct gagctgaatg ctactaatga agcgattaaa
     5461 gttttgctaa agtttttcat tttcatagga aatctcttaa aaaatatata ttttagaatg
     5521 aataattcat aaaagttata agaagaaagt gcagatttta tgtgtcgaag tactctctat
     5581 caattaaatt tcaactgttc cgatcagtta ggagcaaata agagtgaaat tgtgcggtct
     5641 atgaactgtt tatctttggt attttggtat tttcggctct tttttcgtat a
//
LOCUS       DANLZC010000145         5627 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.145, whole genome shotgun sequence.
ACCESSION   DANLZC010000145 DANLZC010000000
VERSION     DANLZC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.145"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..728)
                     /locus_tag="PXE44_002185"
     CDS             complement(<1..728)
                     /locus_tag="PXE44_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5589655.1"
                     /translation="MFYSNNPLIKHKTGLLNLAEELGNISQACKVMGMSRDTSYRYQQ
                     AVEQSGVDALLNQNRRVPNLKNRVDEATEQAVVKFAFDNPAFGQVRVSNELRKQGIFI
                     SAGGVRSIWLRHNLANFKQRLNALEKAVAEKGIILSENQVQALERKKEDDVACGEIET
                     AHPGYLGSQDTFYVGNLKGVGRIYQQTFIDTYSKVAFAKLYTMKTAISAADMLNDKVL
                     PFFESQGLPMLRILTDRGSEYCGKV"
     gene            complement(827..1057)
                     /locus_tag="PXE44_002186"
     CDS             complement(827..1057)
                     /locus_tag="PXE44_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589656.1"
                     /translation="MSLLKKAQTFISIFNEVRAKNEAQRQSSNNLNVALKNAAEMRAE
                     MNGFSLKPQKRSTYFADHYGCGIHAVETYDNV"
     gene            complement(1178..1495)
                     /locus_tag="PXE44_002187"
     CDS             complement(1178..1495)
                     /locus_tag="PXE44_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5589657.1"
                     /translation="MLSRKEVLMTRRASMNLQQIIRNVVEYTYSYESGDKLSAEIHNT
                     LDNLSYFPRIGVIGSVENTRECFCRGYRIIYRETENQVQILAIIHCRQQYPTDYYRSA
                     FKA"
     gene            complement(1488..1646)
                     /locus_tag="PXE44_002188"
     CDS             complement(1488..1646)
                     /locus_tag="PXE44_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589658.1"
                     /translation="MTEEYQNFLIEQVKQGLDDIENGRVISLEESRQLCQKTIYETLQ
                     ELQGAVYA"
     gene            complement(1700..1855)
                     /locus_tag="PXE44_002189"
     CDS             complement(1700..1855)
                     /locus_tag="PXE44_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589659.1"
                     /translation="MLFKEKGYDEFLTQKIAKGRAELESGKGIPLEQATLLVQSTIEE
                     TAQELDD"
     gene            complement(1993..2250)
                     /locus_tag="PXE44_002190"
     CDS             complement(1993..2250)
                     /locus_tag="PXE44_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589660.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFYNYQQFMPEVEAKESLR
                     EQEPELDDGYNEEEYSEEEYSDENDASDENDNYDETYDDNF"
     gene            2264..2440
                     /locus_tag="PXE44_002191"
     CDS             2264..2440
                     /locus_tag="PXE44_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589661.1"
                     /translation="MAYVRIYQGEAYGWCAEKGEANTECPEALLVSLTGDIFEAVGGD
                     KLKGAKKWQPYKKE"
     gene            2444..2590
                     /locus_tag="PXE44_002192"
     CDS             2444..2590
                     /locus_tag="PXE44_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589662.1"
                     /translation="MDTTQKRVALFIIGLSVVMWLRKHNCCTVIFVLLLAALAFSYTM
                     EHLY"
     gene            2606..2812
                     /locus_tag="PXE44_002193"
     CDS             2606..2812
                     /locus_tag="PXE44_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589663.1"
                     /translation="MVKNVVHEEILHNRAELQQAVEKEEFCAKCDNSVVFHLRDNYHE
                     FTLGLDTILQCLHFAEEKGAIRIP"
     gene            2809..3585
                     /locus_tag="PXE44_002194"
     CDS             2809..3585
                     /locus_tag="PXE44_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589664.1"
                     /translation="MNIEYSLNHCQGDGVAFYGRLGTDDLIQLFNHLNPQKRKQKMFA
                     NLLTHIESWETHDDIGFEIIRNRFGYRYSHWNTMELYAKTADQLVFFNDSDARRNWYF
                     PTSKVYKYKGLWDEFIQDLEQYIKGTSREMESVGYQIIEASPYQTETVFKFNTENYLV
                     ELKRKPSEFYSELVDWAFGDMCDFEATIEQICQGQLCYADFEATVTDKKSEIQLGLAR
                     LSGISYSPNDRSFSGYRQELIGEAIASARHNAARFAYLHA"
     gene            3700..4112
                     /locus_tag="PXE44_002195"
                     /pseudo
     CDS             3700..4112
                     /locus_tag="PXE44_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
     gene            4126..4281
                     /locus_tag="PXE44_002196"
     CDS             4126..4281
                     /locus_tag="PXE44_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589665.1"
                     /translation="METRIVKVIYDDGDYFFTRINADEQAIRNYYAIGSSIGFDDIRK
                     IIAVEIL"
     gene            4361..5335
                     /locus_tag="PXE44_002197"
     CDS             4361..5335
                     /locus_tag="PXE44_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL5589666.1"
                     /translation="MNTQPKFDLYQHITDRIVVALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRPDE
                     REKLDEEGNIVYDEEGNPEMERHFFINKHNLFNLDQCENLPVELYDPVSEPKQPKSEP
                     NQYDIFAEIRQIIKGMDLQVEMKPSNRAFYQPGEDKVVMPEMKQFANAQAFHSVLLHE
                     MTHATGANNRLNREGITSGKAKFGNKVYAFEELIAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVLKEDKRAIVRASGKAREACQYMLDALNVAQLADKYDVFEEAA"
     gene            5422..5619
                     /locus_tag="PXE44_002198"
     CDS             5422..5619
                     /locus_tag="PXE44_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589667.1"
                     /translation="MTKSTEIPRGHDNWVITGRDNDGKWHKERFLYYSRREAIQQFRR
                     KHNCVGKHSVIESITPQFGWY"
BASE COUNT         1661 a         1034 c         1160 g         1772 t
ORIGIN      
        1 accttgccac agtactcact gcctcggtcg gtaagaatac gcaacatcgg taatccttgg
       61 gactcaaaga acggtaggac tttatcattg agcatatctg ctgcactaat tgctgtcttc
      121 atcgtataca gttttgcaaa tgcgacttta ctataagtat caataaaggt ctgttgataa
      181 atgcgtccaa cccccttaag attccccaca tagaaggtat cttgtgaacc tagatagccc
      241 ggatgagccg tttcaatttc gccacaagcc acatcgtctt cttttttacg ctccaaggct
      301 tgaacttggt tttcactcag aataatgcct ttctcagcca ctgctttttc taacgcattt
      361 aaacgctgtt taaagttagc gagattatgg cgtagccaaa tagaacgcac accacctgct
      421 gaaataaaaa taccttgttt acggagttcg ttactgactc gtacctgccc aaatgccgga
      481 ttatcgaatg caaacttcac aacagcttgc tctgttgcct catccacacg attttttaga
      541 ttaggaacac gccgattttg attaagtaag gcatccaccc cactttgctc aactgcttgt
      601 tgatagcgat aggatgtatc tcgactcatc cccatcacct tacaggcttg agagatattg
      661 cccagttctt ctgctaaatt taataaaccg gtcttgtgtt taatgagagg gttgttagaa
      721 taaaacatga gagtttcctt ttttgtttag attgaatttg gacactcata ttctaaacgg
      781 gaaactctca ctttttagag tgaattgtca gatcaagtct gatcttctac acattatcat
      841 aggtttctac tgcatgaata ccacaaccat aatggtcagc aaagtaagta gaacgctttt
      901 gaggcttaag ggagaaccca ttcatctcag ctctcatttc agcagcattt ttcaaggcaa
      961 catttaaatt gtttgaagac tggcgttgag cttcattttt agctcgcact tcattaaaaa
     1021 tagagataaa cgtttgtgct tttttaagta gagacattgt ggttctcctg ttttttgcac
     1081 ctaatattat tgaactatgg taggcttggc gagcattgcc ttgagtttta attcggctac
     1141 ggtcattttt ctttttattt ccattttata gctcctgtta tgctttgaat gcagagcgat
     1201 aatagtcggt tgggtattgt tgacgacaat ggataatggc taaaatttgc acttgatttt
     1261 ctgtttctcg atatataatt ctataacctc ggcaaaagca ttctctagta ttttctactg
     1321 aacctataac accaattcgt ggaaaataag aaagattatc caaagtgtta tgaatttccg
     1381 cagataattt atcaccactt tcatagctat aagtatattc cacaacatta cgaatgattt
     1441 gttgtaaatt catacttgct ctgcgagtca ttaacacctc ttttcgacta agcatacacg
     1501 gctccctgta gctcttgcaa ggtttcatat atggtttttt gacacagttg acggctttct
     1561 tctaatgaaa taacacgtcc gttttctata tcatcaagtc cttgttttac ttgctctatt
     1621 aagaagtttt ggtattcttc tgtcatggat aactcctatc attagttgta tatagatata
     1681 ttagcaatgt tacaaattgt taatcgtcca gttcttgagc agtttcttca atagttgatt
     1741 gaactaacaa tgtggcttgc tcaagaggaa taccttttcc ggattctaat tcagctctgc
     1801 cttttgcaat tttttgagta aggaactcat cgtagccttt ttctttaaat agcattttat
     1861 gttcctttca cttattacaa ggctttcagg catagggatt atgttctcct aaatgcgaaa
     1921 tattggaata atttacgctt aaagcaatag agtgtcaaga gcagggtttt tggaagtgtg
     1981 gtttgggaat atttaaaaat tatcatcata agtttcatcg tagttatcgt tttcatcgct
     2041 ggcatcattt tcatcggaat attcttcttc gctatattcc tcttcgttat atccatcatc
     2101 aagttcaggt tcttgttccc ttagtgattc tttagcttca acttcaggca taaattgctg
     2161 gtaattgtag aaaaaagtgc catcgctgcc tttttcccaa agtccggcgt agttgtattt
     2221 tacatattca ggatcagcgt ggcatatcat ccctaattga ggggtggcat atgttcgtat
     2281 ttaccaaggt gaggcttatg gttggtgtgc tgaaaaagga gaggcaaata cagaatgccc
     2341 tgaggcctta ttggtgagtt taaccggcga tatttttgaa gcagtcggcg gagataagct
     2401 gaaaggtgcc aaaaaatggc agccttacaa gaaagaatga cagatggata cgacacaaaa
     2461 acgtgtggct ttattcatca ttggattatc tgttgtgatg tggctaagaa agcataactg
     2521 ctgtacagtg atatttgtat tgttgttggc agcgttagca tttagctaca cgatggaaca
     2581 tttatattag ctggggaact gaattatggt aaagaatgtg gttcatgaag agatattaca
     2641 taaccgtgca gaattacagc aagcggttga aaaagaagaa ttttgtgcaa aatgtgataa
     2701 ctcagtagta ttccatttac gtgataatta tcacgaattt acgcttgggt tagatactat
     2761 attgcagtgt ctgcattttg ctgaagagaa gggagctatc cggatacctt gaatattgag
     2821 tattcactca atcattgcca aggagatgga gtcgcatttt atggtcgact aggtacagat
     2881 gatctaatcc agcttttcaa tcatctcaat ccacagaaac gtaaacagaa aatgtttgct
     2941 aatttgctaa cgcacattga gtcttgggaa acccacgatg atattggttt tgaaattata
     3001 cgaaatcgct ttgggtatcg ttactcgcat tggaatacga tggagcttta tgctaaaact
     3061 gcagatcagt tagtgttttt caatgatagt gatgctcgaa gaaactggta ttttccgact
     3121 tcaaaagtgt ataaatacaa aggcttgtgg gatgagttta ttcaggattt agaacagtat
     3181 attaagggta cttcccgtga aatggaatcg gtcggttatc aaattattga agcctcgccg
     3241 tatcaaacgg aaaccgtttt caaatttaat acggaaaact atctagtaga actgaaacgt
     3301 aaaccttctg agttttattc agagcttgtt gattgggcat ttggtgatat gtgtgatttt
     3361 gaagcaacta tagagcagat ttgtcaaggg caactctgtt atgctgattt tgaagcaact
     3421 gttaccgata agaaaagtga aattcagtta ggtcttgctc gcttgagtgg aataagttac
     3481 tcgccgaatg accgttcgtt tagtggttat cgacaagagc ttattggtga ggcgattgcc
     3541 tctgctcgcc ataatgcagc acgttttgcc tacttgcacg cctaatttgc agatttgtac
     3601 ttcaatttga ccgcttgtag tggttaattt tcaccccgtg gggaaatatt ttccctattg
     3661 ggcaagatgt ttctccactt tttaatttgg agaaatgtga tgaaaaatga acacaattta
     3721 gagcaactta aatatatttg ggataaccgc aaccttgaaa ataaagccta tcaatacctg
     3781 tttcacattt gcattgagcc gatgaaaaaa ggagattgga atcgttatac gatttggctt
     3841 aattttcacg gcaacttaat caacgtgtca gacgttgtat atcgagttgt ggaatctcaa
     3901 aagctcaagg atgacatcaa aatgtggtat gacaataata ccattgtcgt ccgataaaag
     3961 ttgaaacaac aggcttcatc tcatatttac tgcaccagtt ctatcagggg atagcctata
     4021 ttttactgga acagggaatg attactgaaa ctgaagcagc cgagaaaagt tataacttca
     4081 gagctgcaaa ttgttttcaa cgtttgcttt aatggaggtg agataatgga aactcgcatt
     4141 gttaaagtta tttacgatga tggtgattat ttctttaccc gaatcaatgc tgatgaacag
     4201 gctatccgta attactacgc cataggcagt tcaataggtt ttgatgatat aagaaaaatt
     4261 atcgctgttg aaattttata aaccacccat aggggaaact atttccccat agggtgatag
     4321 cgtttcccca tttttctacc cgaactttgg agaaacactt atgaacactc aacctaaatt
     4381 tgatttatac caacacatca ctgaccgaat tgttgtagca ctggaacaag gtacagcccc
     4441 gtggttaaaa ccgtggagcc atccggaatg caatatggca ttacctcgca atgcggtatc
     4501 taaccgcttg tattccggta tcaatacact cttactctgg attgctactg ctgaacatgg
     4561 ttacaaacag tgcaagtgga ttacagcaaa aaaagccaat gaactcggcg gatatatccg
     4621 taaaggggaa aaagctactg tggtgatgaa ctaccgtcct gatgagagag agaaactcga
     4681 tgaggaaggg aatattgtgt atgacgagga aggaaatccg gaaatggaac gacacttttt
     4741 cattaataag cacaatctgt tcaatctgga ccagtgtgaa aatttaccgg tggaactgta
     4801 cgatcctgta tcagaaccga aacaacctaa atctgagcca aaccaatacg atatttttgc
     4861 tgagattcgg cagattatca aaggaatgga tttgcaggtg gaaatgaagc ctagcaatcg
     4921 ggcattttat caaccaggtg aagataaggt cgtaatgccg gagatgaagc agtttgcaaa
     4981 tgctcaagcg tttcatagtg tattgttaca cgaaatgact cacgcaacgg gggcaaataa
     5041 tcggcttaat cgggaaggta ttacttccgg aaaggctaaa tttggaaaca aagtctatgc
     5101 ttttgaggag ttgattgctg aaatgggcgg tgcattttta tgtgcctacc ttggttttga
     5161 tactgtgcct caaaatgcgg cgtatattga aagttggatt cgagtattga aagaagataa
     5221 acgggcgatt gtccgtgctt ccggaaaagc tcgtgaagcc tgccaataca tgttagacgc
     5281 attaaacgtc gcacagttgg ctgacaaata cgatgtattt gaagaagctg cttaacaaat
     5341 ttgccgaaag gcatatctca aggggagacc tatccctttg ggcatagtgt ttccctattt
     5401 tttattaaat ggagaaaagc tatgacaaaa tcaactgaaa tacctcgtgg acatgataac
     5461 tgggttatta caggcagaga caatgatggg aaatggcata aagaacgttt tctttattat
     5521 tctcgccgtg aagccatcca acagtttcgt cgaaaacaca actgcgtagg taaacatagt
     5581 gttattgaat caataacacc tcaatttggc tggtattaac cggcttt
//
LOCUS       DANLZC010000146         5477 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.146, whole genome shotgun sequence.
ACCESSION   DANLZC010000146 DANLZC010000000
VERSION     DANLZC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.146"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            123..293
                     /locus_tag="PXE44_002199"
     CDS             123..293
                     /locus_tag="PXE44_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589668.1"
                     /translation="MKNQRNDTQKSSLWQEFEYRKRLKNLIESGLSVAEIETRSQQIY
                     EELKSECTTEAN"
     gene            268..1136
                     /locus_tag="PXE44_002200"
                     /pseudo
     CDS             268..1136
                     /locus_tag="PXE44_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            1133..2494
                     /locus_tag="PXE44_002201"
     CDS             1133..2494
                     /locus_tag="PXE44_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HDL5589669.1"
                     /translation="MSQNLKNITYEVEYLLVGALLKSGLNSKARDVLSWLEPEMFATF
                     QLGVIYEAIRKQALKDNVIDMLLLNTDYGQDFATLAEIMKNTISGANLDGYAEKVRQY
                     HQRREAQRVFLDVAGELQNARDEQLDAITSTGLAHLSKLLQRGGKVKPIDMNDLLEGY
                     LELFQERAKPSFKERLLFTGIEALDGKLGGINDTDICIVAGRAGNGKTETAITFTKNI
                     IENKGSVLFFSLEMSKEQIMDRLIASASGVNSVKLRNPEMMNDEDFARMGTAIQPLQN
                     QQLYIVDKSGLTAEEIVAIAESHIQEYGKVSAVVIDYIGLVRHGKLDGKINRTYQIGE
                     SMERFKTFCKNNHTPMILLAQLNRNADGSRPTNADLRDSGSLEQDASQIIMVHNQRNK
                     DGEPAPYTEWIVTKNRFGANGTVYMQFQQGRFVECDQAEAREYFQPKETKTSSNKRYG
                     AMQ"
     gene            2491..3027
                     /locus_tag="PXE44_002202"
     CDS             2491..3027
                     /locus_tag="PXE44_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL5589670.1"
                     /translation="MTDFDKNIKYKIIYADPPWKYKDSGCNGSAENHYTTMNIKEICD
                     LPVQDISDKDSILFLWVTYPMLPEGLKLIEAWGFNYKTIGFQWVKTNKKNKNSFFFGL
                     GRWTRGNTECCLIATKGKPSRVSNKVSQLIIEPIQHHSKKPDIVREKIVELMGDLPRI
                     ELFARNKTQGWDVWGDEV"
     gene            3118..3333
                     /locus_tag="PXE44_002203"
     CDS             3118..3333
                     /locus_tag="PXE44_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589671.1"
                     /translation="MITYKCPKCNGELEDLSINDEWGWFLDEPYRCNGHYTGRFPNIS
                     RDSTLNRTKSCGYFSKEEVKKVNGTQR"
     gene            3317..3823
                     /locus_tag="PXE44_002204"
     CDS             3317..3823
                     /locus_tag="PXE44_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL5589672.1"
                     /translation="MAHSVNDIVKAHGRRYKARLKIQMIKMQGGVLVPLNEREAEALV
                     SLKNGEQYEIEVIRTRNPAFHRKVFAFLNFCFEYWSADKTEWEFFDERKQFDTFRKNL
                     TVLAGFKEITYTLDGRMRVEAQSLSYGNMEQDEFEQVYKALINAAIKHVFNNTKDENR
                     INQLYSFF"
     gene            3873..4085
                     /locus_tag="PXE44_002205"
     CDS             3873..4085
                     /locus_tag="PXE44_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589673.1"
                     /translation="MKKNVIRYWLSVPFRIIAVLFFLVILTVRYVGYLLIYPLAFFAA
                     VGSFIVFCQWTYQEWLYYFKRGFAPI"
     gene            4131..4325
                     /locus_tag="PXE44_002206"
     CDS             4131..4325
                     /locus_tag="PXE44_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589674.1"
                     /translation="MTKFFETIAFVMLFGIIPSCLLAINTTLMNMNIKNAPWFMVIIQ
                     LGFNITCYWLWFISVMRNVI"
     gene            4453..5022
                     /locus_tag="PXE44_002207"
     CDS             4453..5022
                     /locus_tag="PXE44_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDL5589675.1"
                     /translation="MANKPPKQHKCKECGSYYIKFQSTQQVCSVKCAMAMGKRKTEAK
                     RKKSAKIDRLEQRKRLEKLKSRRDFEKDLQKIFNKFIRLRDKDLPCISCGRYHQGKYD
                     AGHYKTVGGNPELRFNEDNCHSQCVPCNRHLHGNIVNYRVNLIEKIGLERVEFLERKD
                     HPPLKLTIEQIKDLIKVYKAKCKELERVT"
     gene            5012..5464
                     /locus_tag="PXE44_002208"
     CDS             5012..5464
                     /locus_tag="PXE44_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589676.1"
                     /translation="MLPRKIKNKWLEPEKQKWIEELLRLWGAWEFGGLDLESRVNMIY
                     RLMKSAEGVKSLSSREVCNDSLGTLINEIFTVVTKRDVMLADILKQKYWFGRSERQIA
                     IYYQLRDSEGRKERRWQEIIQDKCKKAERIIADILESKISSSNSTDKL"
BASE COUNT         1812 a          888 c         1285 g         1492 t
ORIGIN      
        1 attctaccac agtaaaaatt caatttttat tagagtggca atttttatgc tgcttgcgtg
       61 gtgggttttt aacaattaaa aaaccaccgc tggaacggtg gtaatttaca aggagttacc
      121 ttttgaaaaa tcaacgcaat gatacacaaa aaagctcctt atggcaagag tttgaatatc
      181 gaaaacggtt aaaaaatctc attgaaagcg gtttatccgt agccgaaatt gaaacccgtt
      241 cacaacaaat ttatgaggag ctgaaaagtg aatgcacaac cgaagcaaat taacccaatt
      301 ttaaaattgc accctaaaca atcagaggct aagaaagtga gtgttgatga tggttataca
      361 caaattccga atgagttatt aaaagcaatt ttgcgatcgg gtgttttagg ttggaaaggc
      421 tcttattact ggctaccgtc ttaaaaacat tgtcttggca taaagaaagt gattggttca
      481 cgcattccca agtgtgtgaa atgatgaata ttgagccgac aaaataccat attaatcaac
      541 tttccgctgc tcgaaaagaa cttatccgtg aaaatatctt gtttgaagat ggtaaggaaa
      601 caggggtaaa tctcagtgtt tttgaatgga aaatggtata tcccgaaaaa gtagggagtt
      661 cccgaaataa tagggaatta attcccgaaa aagtagggaa tgggtatccc gaaaaagtag
      721 ggaacacaaa agaaactatt acaaaagaaa aaataaataa tgaatttact aacgtaaatt
      781 ctgtgcaaca gcccgagacc tctttgccga aaaaaacaaa atctgagccg gtcgattatc
      841 aaggagtgat ggaagaattt aatcgtgctg tggaagacac accgattccg caaattcggg
      901 cgatgagtga tgagcgaaag cgatcggttc acgcattagc aaaaatcttg cgaaaagaat
      961 ttggtggcta caccagacaa cactttgccg attacttcaa tgattttgtc cgacaggcaa
     1021 gtagccggaa agacagattt tattttggcg ggcttgatgg ctcggcttgg gtagcaaatt
     1081 tcggctacat cattcgttcg aaaacgtttt ataagacttg ggaagattcg ctatgagcca
     1141 aaatttgaaa aatatcactt acgaggttga atatttgctg gttggagcat tgctgaaatc
     1201 cggtcttaac tcgaaagcta gggacgtatt gagctggtta gagcctgaaa tgttcgctac
     1261 ctttcaactt ggggtgattt atgaggctat ccgcaagcag gcgttgaaag ataacgtgat
     1321 cgatatgctg ttactcaata ccgactacgg acaagatttt gccacgttgg cagagataat
     1381 gaaaaacacg atcagcggtg caaacttaga cggctatgca gaaaaagtcc ggcaatatca
     1441 tcaacgccga gaagcccagc gagtgttttt agacgttgca ggtgagttgc aaaatgcccg
     1501 agacgagcag ttagatgcta tcacttcaac cgggttagct cacctaagca aattattaca
     1561 acgtggcgga aaagtgaaac cgattgatat gaatgatttg ctcgaaggtt atttggaatt
     1621 gttccaagaa cgtgcaaaac caagttttaa agagcgtttg ttgtttaccg gtatagaagc
     1681 attagatggg aaactaggcg gtattaacga taccgatatt tgcattgtag caggacgtgc
     1741 cggtaacggt aaaaccgaaa cagccatcac gtttactaaa aacattatcg agaataaagg
     1801 ttcggtgctg ttcttctcgc tggagatgag caaagagcag attatggata ggctgattgc
     1861 cagtgcaagc ggtgtaaatt cagtaaaact tcgcaatccg gaaatgatga atgatgaaga
     1921 ttttgcccga atgggaacgg cgattcagcc tttacaaaat cagcagttgt acattgttga
     1981 taaaagcgga ttaaccgcag aagagattgt ggcgattgca gagagccata ttcaagagta
     2041 cggcaaagtg agtgcggtag tgattgatta tatcggtttg gttcgacacg gcaaactaga
     2101 cggcaaaatt aaccgaacct atcaaatcgg tgaaagtatg gagcggttta aaactttctg
     2161 caaaaacaat cacacaccga tgattttatt ggcacagctt aaccgcaatg cagatggcag
     2221 tcgcccaacc aacgcagatt tgcgagacag tggcagtctt gaacaagatg caagccaaat
     2281 cattatggtg cataaccaac gcaacaaaga cggcgagcca gccccctata cagaatggat
     2341 tgttactaaa aatcgctttg gagcaaatgg cacggtttat atgcaattcc agcagggtag
     2401 gttcgtagaa tgcgaccagg cagaagcgcg ggagtatttc cagccgaaag aaactaaaac
     2461 atcatcaaac aaacgctatg gagcaatgca atgacagatt ttgataaaaa tataaaatac
     2521 aaaattattt acgcagatcc gccttggaag tataaggata gtggctgtaa tgggagtgca
     2581 gaaaaccatt acacaacaat gaatatcaaa gaaatatgtg atttacccgt ccaagatatt
     2641 tcagacaaag attctattct gtttttgtgg gttacttacc ctatgttacc tgagggctta
     2701 aagttgattg aggcgtgggg gttcaactat aaaacaatag ggtttcaatg ggtgaagaca
     2761 aataagaaga ataagaattc ttttttcttt ggcttgggac gatggactag aggtaatacc
     2821 gaatgctgtt taattgcaac aaaaggaaag ccatcgaggg taagcaataa agtgagccaa
     2881 ttaattattg agccaataca acatcatagt aaaaagcctg acattgtcag agaaaagata
     2941 gttgagttga tgggtgactt gccaagaata gagttgttcg ccagaaataa aacccaaggt
     3001 tgggatgttt ggggggatga ggtgtgattg tttcaagcta ttgttaccca aagtgcggta
     3061 gggttttatt aaaaaggttg gcggttgtga aggataacga aggttgggac ggtttagatg
     3121 attacttata aatgccccaa atgcaacgga gagcttgaag atttaagtat taatgatgaa
     3181 tggggttggt ttttagatga gccgtatcgt tgcaacggac actatacagg gcggtttcca
     3241 aacattagcc gagatagcac gttaaatcgc acaaaatcct gtgggtactt tagcaaggaa
     3301 gaggttaaga aagtaaatgg cacacagcgt taatgacatc gtaaaagccc acggcaggcg
     3361 ttataaagca agattgaaaa tacagatgat aaaaatgcaa ggaggcgttt tagtccccct
     3421 taatgagcgt gaagctgaag cattggtatc acttaaaaat ggcgagcaat atgaaattga
     3481 ggttattcgc actcgcaatc cagcctttca tcggaaggtc ttcgcctttt taaatttttg
     3541 ctttgaatat tggtcggcag acaaaaccga atgggagttt tttgatgaga ggaaacagtt
     3601 tgatactttc cgaaaaaatc tgactgttct ggcaggattt aaagaaatca catacaccct
     3661 agatgggcgt atgcgtgtag aagctcaaag tcttagttac ggaaatatgg aacaagacga
     3721 gttcgagcag gtttacaaag cattgattaa tgcagctatc aagcacgttt tcaacaatac
     3781 caaagatgag aataggataa atcagttgta ttcgtttttt taattaaggc gaaatcgcca
     3841 tatttaatta ggtttaattg caggagatta aaatgaaaaa gaatgttatt agatattggc
     3901 ttagcgtgcc attcagaatt attgcagtgc tttttttctt agtcattctt actgttagat
     3961 atgtaggtta tttattgatt tatccattag cattctttgc agcagtcggt agttttattg
     4021 ttttctgtca atggacatat caagaatggc tttattattt taagcgcggt tttgctccga
     4081 tttagtgtgt gtttattaat tccctcgaat tcgggggaag gagaagtaag atgacaaaat
     4141 tttttgaaac tatcgcattc gtgatgttat ttggcattat tccaagctgt ttgttggcga
     4201 ttaatactac tttaatgaat atgaatatta aaaacgcacc gtggtttatg gtgattattc
     4261 agttaggttt taatatcact tgttattggt tgtggtttat ttctgttatg cggaatgtta
     4321 tttaaggaga ttaataagtg agccagtgca atgaactgac tgtaatttta atcgccatcg
     4381 ctattcttag tgtatgtgcc ggttcactct tttttattga gtggtttgat aagagagatg
     4441 gtgagggttg gaatggctaa caaaccacct aaacaacaca aatgcaaaga gtgcggcagt
     4501 tattacatca aatttcaaag cacgcagcag gtctgctctg ttaagtgtgc tatggcaatg
     4561 ggtaagcgta agacagaggc gaaacgcaaa aaaagtgcaa aaatcgaccg cttggagcaa
     4621 cggaagcggc tggaaaaatt gaaaagccgt agagattttg agaaagattt acaaaagata
     4681 tttaataaat tcattcgctt gcgtgataaa gatttaccct gtatttcctg tggccgctat
     4741 catcagggga aatatgatgc agggcattat aaaaccgttg gtggtaatcc tgagctacgt
     4801 tttaatgaag ataattgcca cagtcaatgt gtgccgtgta accgacatct gcacggcaat
     4861 attgtgaatt atcgagtaaa tctgattgag aaaatcgggc ttgagcgtgt ggaattttta
     4921 gagcgtaaag accacccacc attaaaactt actatcgaac aaatcaaaga tttaatcaaa
     4981 gtctataaag cgaagtgcaa ggagcttgag cgtgttacct agaaaaatta aaaacaagtg
     5041 gttagagcct gaaaagcaga aatggatcga agagttgcta aggttatggg gggcgtggga
     5101 atttggcgga ttggatttag aaagtcgggt aaatatgatt taccggctga tgaaatctgc
     5161 ggagggggtt aaaagtcttt catctagaga ggtttgtaac gatagcctcg gcacgctgat
     5221 caatgagatt tttacggtag tcaccaaaag agacgtaatg ttagccgata tactcaaaca
     5281 aaaatactgg ttcgggcgtt cggagcggca aatcgctatt tattatcaac taagggacag
     5341 cgagggtaga aaagaaagac gatggcaaga aattattcaa gataaatgca aaaaagcgga
     5401 aagaattatt gcagatattc ttgaaagcaa gatttcatcc agtaattcta cagataaact
     5461 gtagaagatc agacttg
//
LOCUS       DANLZC010000147         5467 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.147, whole genome shotgun sequence.
ACCESSION   DANLZC010000147 DANLZC010000000
VERSION     DANLZC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.147"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..509
                     /gene="hutZ"
                     /locus_tag="PXE44_002209"
     CDS             <1..509
                     /gene="hutZ"
                     /locus_tag="PXE44_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme utilization protein HutZ"
                     /protein_id="HDL5589677.1"
                     /translation="RQEVLQNRLGPEIEELKQQIKTIMLATIDKDGVPNVSYAPFVIN
                     NGEYQVLITTIARHARNLQEVPKVSLMLIEDESKSRQIFARRRLTFDATVRMIERTDE
                     EWNNSIAALKARHGDLVDELSNMQDFKLFSFKPTAGLFVKGFGKAFDVGVDDLINVVH
                     LDQGHQTA"
     gene            659..3271
                     /gene="pepN"
                     /locus_tag="PXE44_002210"
     CDS             659..3271
                     /gene="pepN"
                     /locus_tag="PXE44_002210"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDL5589678.1"
                     /translation="MQPKAKLRKDYRAPDFTITDIYLDFQLDPDRTFVTSKLTVVRKN
                     PEATTLRLDGHSFDFLSLKYNGEPFSAFQKDDESLTLNLADVPAERFELEIETALNPA
                     QNTSLQGLYKSGDGFCTQCEAEGFRQITYMLDRPDVLAKYTTKITASKSKYPYLLSNG
                     NHIAQGDLHDGRHWVEWQDPFFKPSYLFALVAGDFDLLQDKFITKSGREVTLEIYVDR
                     GNLDRAPWAMESLKRSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKF
                     VLAKPETATDTDYLDIEAVIAHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQEFTSD
                     LWSRSAKRIEDVRLLRTVQFAEDASPMAHPIRPEKVIEMNNFYTVTVYEKGAEIIRMI
                     HTLLGEEKFQQGMQLYVARHDGSAATCEDFVKAMEDASGVNLEQFRRWYSQSGTPELT
                     ISDEYDETRKAYRLHISQMTPATHDQMDKLNLHIPLKVALYGEQDGKRIPLQYELLTV
                     SDVLDVTAEHQTFEFHNVTQRPVPALLCDFSAPVRLDYNYTTEQLLTLLKHAENDFVR
                     WDAAQMLYTNELRENLSRYQQHQPLSFSEGLVNALTFVLDNSASLPELTALTLTLPKE
                     TEFAESFKTIDPQGIAVVREFMQHSIAHALQDKLLAVYHQNQCGEYQVVAEDIAKRAL
                     RNACLSYLAFTDLGNALVHKHYNNADNMTDTLAALQAATKAQLSCRDALLADFEQKWK
                     HDGLVMDKWFMLQATRPDDDVLSIVQNLMEHPSFNFNNPNRLRSLVGAFCGQNPKAFH
                     AIDGSGYRFLVDTLIKLNESNPQVAARLIEPLIKLSRYDNQRQTLMRRGLERLRELDN
                     LARDLFEKIEKALD"
     gene            complement(3331..3603)
                     /locus_tag="PXE44_002211"
     CDS             complement(3331..3603)
                     /locus_tag="PXE44_002211"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL5589679.1"
                     /translation="MVAKLFSVYGRVQGVAFRFFTLQEANRLGIKGYVRNRDDGSVEV
                     VAEADEQTIAQLENWLQQGSPAAKVERVIVSDYLGSERFKRFEVRY"
     gene            complement(3657..5291)
                     /locus_tag="PXE44_002212"
     CDS             complement(3657..5291)
                     /locus_tag="PXE44_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700110.1"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDL5589680.1"
                     /translation="MQSKFSLSLLTTSLVLGLSANVFAAKVPEGTTLAEKQEITINNG
                     FEPSSFDPHKIEGVPESQVAYQLFEGLVTKDSDGNIQPGVAESWENTPDYKTWTFKLR
                     QDAKWSNGDAVTAHDFVFAWQRLVNPLTASPYSSYLTYLNVENAQDIIDGKKKPEELA
                     VKAIDDHTFQVQLSQPVPYAVELATHSSLLPVHKATVEKHGDAWVQKGNLVGNGAYTL
                     ADHVINEKIVFERNKNYWNDKETVINKATFLAIANAATDVSRYRAGDLDMTNYALPPE
                     QFATLKKEIPNEVFTARTLSTYYYEINHKRAPFDNVKVREALNLALDRTIITDKVLGQ
                     GQTPTYVFTPPYIHEGEKIQQPAYSKDSMADRKAKALKLLEEAGYSKANPLKFTILYN
                     TNDNHKKIAIAVSSLWKQGTDGIVDVKLENQEWKTFLDTRRNANYDVSRAGWSADYNQ
                     ATTFVNYFLSNSSNNTAFYKSQAYDDAVAESYKATDAAGRAAAYAKAEEQLAKDYAII
                     PIYNYVNPRLVKPYVKGYEGKDPQDDILLRNLYIVK"
BASE COUNT         1597 a         1174 c         1089 g         1607 t
ORIGIN      
        1 accgtcaaga ggtcttacaa aaccgtttag gtccagaaat tgaagaatta aaacaacaaa
       61 tcaaaactat aatgcttgca accatagata aagatggtgt accgaatgtg agctatgccc
      121 cttttgtaat taataacggc gaatatcaag tcttaatcac tactattgcc cgtcacgctc
      181 gtaatttaca agaggtgcca aaagtctctt taatgctaat tgaggatgaa agtaagagcc
      241 gtcaaatttt tgcccgtcgt cgcttaacct ttgacgcaac agtgcgaatg attgaacgta
      301 ctgatgagga atggaataat agcattgcag cacttaaagc tcgtcacggc gatttagttg
      361 atgaactgtc aaatatgcaa gatttcaagc tctttagctt taagccaacg gcaggcttat
      421 ttgtaaaagg atttggtaaa gcctttgatg ttggtgttga tgacttaatt aatgtcgttc
      481 atttagacca aggccaccaa actgcctaat tgcgaaataa actcacggct ataaataatc
      541 ctatttatag ccgtttttat tgcgacaagc ggtcaaattt tttggaaaat ttaccgctat
      601 tttagtacaa taacgacact attctatgag ctaaacgcaa ctaaaaaaag gaaatcctat
      661 gcaacctaaa gcgaaactca gaaaagatta tcgagctccc gatttcacta ttactgatat
      721 ttatcttgac ttccaacttg acccagatcg cacttttgtc acctcaaaac taactgtcgt
      781 gcgtaaaaat cctgaggcga caacacttcg tttagacggt catagtttcg atttcctttc
      841 gcttaaatat aacggcgagc cgttttccgc ctttcaaaaa gatgatgaat ctttaacctt
      901 aaatttagcc gatgtgcctg ccgagcggtt cgagcttgaa attgaaaccg cattaaatcc
      961 ggcacaaaac acctcgttac aaggtttgta taaatcgggc gacgggtttt gcacccagtg
     1021 tgaggctgaa ggtttccgcc agattaccta tatgctcgac cgccctgatg tacttgcaaa
     1081 atatacgaca aaaatcaccg ctagtaagag caaatacccg tatctgcttt ctaacggcaa
     1141 ccacattgcc caaggcgact tgcacgatgg cagacactgg gtggaatggc aagatccgtt
     1201 ctttaaacca agctatttat ttgccttggt tgcgggggat tttgacttac tccaagataa
     1261 attcatcacc aaaagcggac gtgaagtcac gcttgaaatt tatgtcgatc gtggaaattt
     1321 agaccgtgca ccttgggcga tggagagctt aaaacgttca atgaaatggg atgaagagcg
     1381 tttcggtttg gaatacgatt tagatattta tatgattgtt gccgttgatt tcttcaatat
     1441 gggagcaatg gaaaacaaag ggttaaatat tttcaattcc aaatttgtat tggcaaaacc
     1501 ggaaaccgca accgatacgg attatttaga tattgaagcg gtgattgccc acgaatactt
     1561 ccataattgg acgggcaacc gcattacctg ccgtgattgg ttccaactaa gcttaaaaga
     1621 gggcttaacc gtcttccgtg atcaggaatt tacctccgat ttatggtctc gttcggcaaa
     1681 acggattgaa gatgtgcgtt tactgcgtac cgtacaattt gccgaagatg ccagcccgat
     1741 ggctcacccg attcgcccgg aaaaagtgat tgaaatgaat aatttctaca ccgtgactgt
     1801 gtatgaaaaa ggggcggaaa ttattcgtat gattcacacc ctgttaggcg aagaaaaatt
     1861 ccagcaaggt atgcaacttt atgtggcaag acacgatggt tctgcggcaa cttgtgaaga
     1921 tttcgttaaa gcaatggaag atgcttcagg cgttaatctt gaacaattcc gccgttggta
     1981 tagccaatcc ggcacgcctg agctcaccat ttccgatgaa tatgatgaaa cccgcaaggc
     2041 ataccgcttg catatttcac aaatgacacc ggcaacccac gatcagatgg ataaactcaa
     2101 tctgcatatt ccgcttaaag tagcgttata tggtgaacag gacggtaaac gcattccgtt
     2161 acaatatgaa ttattgaccg taagcgatgt gttagatgta accgcagaac accaaacctt
     2221 tgagtttcat aatgtaacac aacgcccggt gcctgcatta ctgtgtgatt tctctgcacc
     2281 ggtacggtta gattacaact ataccaccga gcagctttta accttactca aacacgctga
     2341 aaatgatttt gtacgctggg atgcagctca aatgctctat accaatgaac tgcgtgaaaa
     2401 tttaagccgt tatcagcaac atcaaccact cagcttctca gaagggttgg tgaatgccct
     2461 aacgttcgtg ttggataact cagcaagcct gccggaacta actgcattaa ccctcacatt
     2521 gccaaaagaa accgagtttg cagaatcgtt caaaaccatt gacccacaag gtattgcggt
     2581 tgtgcgtgaa tttatgcagc attccattgc tcacgccttg caagataagt tattagcggt
     2641 atatcaccaa aaccaatgcg gggaatatca agtcgtggct gaagatattg ctaaacgagc
     2701 cttgcgtaat gcttgcttaa gctatttagc ctttactgat ttaggcaacg cgttggtgca
     2761 taagcattac aataatgccg ataatatgac tgatacgctt gctgcattac aagccgcaac
     2821 caaagcccag ctaagttgcc gtgatgcatt attagccgat tttgagcaaa aatggaaaca
     2881 tgatggttta gtgatggata aatggtttat gctacaagcc acccgaccgg acgacgatgt
     2941 gttatccatc gtgcaaaatt taatggaaca cccaagtttt aactttaata acccgaaccg
     3001 tttacgttca ttagtaggtg cattctgcgg tcaaaacccg aaagccttcc acgccattga
     3061 cggttcaggc tatcgtttct tggtagatac gctgattaaa cttaacgaaa gtaacccaca
     3121 agttgctgct cgacttattg agccgttaat taaactctca cgctacgata accaacgcca
     3181 aaccttgatg cgtcgtggtc tagaacgcct gcgtgagctg gataatttag cacgagatct
     3241 gtttgagaaa attgaaaaag cgttagatta aaccaagcaa tacaagcggt gtcgtttgca
     3301 aattttttta caaatgacac cgcttgttag ttaatacctt acctcaaacc ttttaaacct
     3361 ttctgagcca agataatcgc tcacaattac ccgttccacc tttgcagcag gtgagccttg
     3421 ttgtaaccaa ttttccagtt gagcgattgt ctgttcatcc gcttcagcta ccacttctac
     3481 cgagccgtca tcacgatttc tgacatagcc ttttatgcct aatcgattcg cttcctgcaa
     3541 ggtaaaaaac cgaaaagcga caccttgaac ccgtccgtaa accgagaata attttgcgac
     3601 cattcttccc ccataaaaat aggctgctta tttttcaata aacagcccat aatgtcttat
     3661 ttcacgatgt agaggttacg cagtaaaata tcatcttgcg gatctttacc ctcatagcct
     3721 ttcacgtaag gtttcaccaa acgtgggttt acatagttat aaatcggaat aatcgcataa
     3781 tctttggcta actgctcctc tgcttttgca taagcagctg cacgaccagc tgcgtctgtc
     3841 gctttgtaag attctgctac cgcatcatca taagcttggc tcttataaaa cgcagtattg
     3901 ttgctggagt tcgataagaa atagttcaca aaggtggttg cttggttata gtctgccgac
     3961 caacctgcac gagaaacatc ataatttgca ttgcggcggg tatctaagaa tgttttccac
     4021 tcttggtttt ctaatttcac atcaacaatg ccgtctgtgc cttgtttcca taatgatgaa
     4081 actgcaatcg caattttctt gtggttgtcg ttggtattat aaagaatagt aaattttaac
     4141 gggttcgctt tactgtaacc ggcttcttcc aataatttca acgctttggc ttttctatct
     4201 gccattgaat ctttggaata agcaggttgc tggatttttt caccctcgtg aatataaggc
     4261 ggtgtaaata cataagttgg tgtttgacct tgtcctagca ctttatcggt aataatggtg
     4321 cgatctaaag ctaagtttaa cgcctcacgc actttcacat tatcaaacgg tgctcgttta
     4381 tggttaattt catagtaata agtagaaagg gttcttgccg taaacacttc gtttggaatc
     4441 tcttttttca aggttgcaaa ctgctccggt ggaagagcat agttggtcat atccaaatca
     4501 ccggcacggt aacgtgaaac atcagttgcg gcattagcaa tggctaagaa agtcgcttta
     4561 ttaatcacgg tttctttatc attccaataa tttttattac gctcaaacac gattttttca
     4621 ttaataacgt gatcggctaa ggtataagcc ccgtttccaa ccagattgcc tttttgcacc
     4681 cacgcatcgc cgtgtttctc aacggttgct ttgtgtacag gtaataaaga agagtgtgtt
     4741 gccagctcaa ctgcatacgg gacaggttgg cttaactgca cctggaaagt atgatcatca
     4801 atcgctttaa ctgctagttc ctccggtttt ttcttgccgt caataatatc ttgagcattt
     4861 tctacattga gataagttaa atagcttgaa taaggtgaag ccgtaagtgg attaaccaaa
     4921 cgctgccaag caaatacgaa atcgtgagca gttacggcat caccgtttga ccatttagcg
     4981 tcttggcgta atttgaacgt ccacgttttg taatccggtg tattttccca actttctgcc
     5041 acacccggct gaatattgcc gtcagaatct ttcgttacca atccttcaaa taactgataa
     5101 gcaacttgcg attccggcac gccctcaatt ttatgtggat caaaacttga tggctcaaaa
     5161 ccattattaa tcgtaatttc ttgtttttct gcaagcgttg ttccttcagg cactttggct
     5221 gcaaacacat ttgccgataa gcctaacact aatgaggttg ttaataagga aagcgagaat
     5281 tttgactgca tatttgatct ccattgatga atattggctt gcaaccagcc gatttgttct
     5341 aattaatttt tttaagtcta ggcattatta ttttgattgc aaggaatttt tttaaaaaat
     5401 gtgatagata tcacctttca atgcaaaaat tgcataaaaa aatcccgaag aaaatagctc
     5461 tataaac
//
LOCUS       DANLZC010000148         5465 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.148, whole genome shotgun sequence.
ACCESSION   DANLZC010000148 DANLZC010000000
VERSION     DANLZC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.148"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            81..215
                     /locus_tag="PXE44_002213"
     CDS             81..215
                     /locus_tag="PXE44_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/trimethylamine N-oxide reductase
                     NapE/TorE"
                     /protein_id="HDL5589681.1"
                     /translation="MRSELLKTLLLSGLILPLLMAFAVGAYGFIVWMLQLFVIGLPGA
                     "
     gene            219..1382
                     /gene="torC"
                     /locus_tag="PXE44_002214"
     CDS             219..1382
                     /gene="torC"
                     /locus_tag="PXE44_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDL5589682.1"
                     /translation="MLGSIKALFTRPATKIGLGVLVGIGFIAGAISWHQFNKAMDHTS
                     TEEFCVSCHSMQQPLEELKKTAHWSNKSGVTATCSSCHLPHDYTEKYARKVQASREAW
                     AELSGKYKEEGSFEKHRLEMAEREWARFAANGSKECKACHSYERMNFDKMGEAARKAM
                     KPAAEKDQSCMDCHKGIAHHLPKADENALKSKFDQFVNKSPNQGKSYYTKGNAELFLD
                     EGLSQAIGQLETAVPVAFVQAGKNADLVELTMWRKEKGFGRIWYHDFGKNITDAVLTK
                     EFMANQPKFEVLETKEDPITGLNWQKVKLQAWIGKAQLLDDVTPIWANAENQYKTQCS
                     TCHRQPDVAHFDSNSWIGLFNGMVGFTNMDKQTGKEVLRYLQMHASDSEEAKH"
     gene            1482..3947
                     /gene="torA"
                     /locus_tag="PXE44_002215"
     CDS             1482..3947
                     /gene="torA"
                     /locus_tag="PXE44_002215"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL5589683.1"
                     /translation="MQQSRRQFLKNMSVMAAAVTMPNFLVPRNVFASEDLSQWKVSGS
                     HWGAMRAKIENGRVAEIKPFEFDKHPTEMLNGIKGLIYSDSRIRYPMVRLDWLKNRHN
                     SNTAQRGDNRFVRVTWDEALDLFYQELERVQKEYGPWALHTANVGWRSTGQFHSCGNH
                     MIRAIAMHGNSVGTAGDYSTGAGQTILPYVLGSTEVYSQGTSWEIILKESENIIFWAS
                     DPVKNLQVGWNCETHEAYAYLEKLKEKVAAKGVNVICVDPVKSKTQNFLGCEQQYINP
                     QTDVPFMLALAHTLYTENLYDKKFLDMYTVGFEKFLPYLLGETEDKVAKTPEWAAEIC
                     GISADKIREFARMLAGKRTQFIFGWAIQRQQHGEQPYWMGAVLASMLGQIGLEGGGIS
                     YAHHYSSIGIPESGAAMPGAFPLNIDEGQTPKYDNKDYKGYSDTIPVARMTDSLLHAG
                     ETIDYNGKKVTYAPFKMAIFTGCNQWHRHSERNKMKQAFQKLETIVSINYSWTATCRF
                     SDIVLPACTPFERNDIDAYGSYSNRGVIAMHKLVDPLYDSRSDFEIFRDLCRRFGKEK
                     EYSRGMDEMQWVEKLYKDCRRENRGKFEMPEFAEFWQKGYVLFPEGKPWVRHADFRED
                     PELHALGTPSGFIEIYSNKIASFGYDDCKGHPMWFEKAERSHGGKNSDKFPYWLQSVH
                     PDKRLHSQLCESKELRETYSVQGREPLYINPQDAAKHGIQDGDLVRVFNDRGQAIVGA
                     VLSDNFPTGVVRLQEGAWYSPLDESVGAIDTYGDPNTMTLDIGTSKLAQAVSANTCLV
                     NIEKFIGNAPAPNGFNGAIEVTA"
     gene            3947..4537
                     /gene="torD"
                     /locus_tag="PXE44_002216"
     CDS             3947..4537
                     /gene="torD"
                     /locus_tag="PXE44_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDL5589684.1"
                     /translation="MTLAISREERQFIYGWFNAMLARELSDEQLNALQAGEFDDFFAY
                     LSELGFAEQVAKFQAEVTACRQLEFPRLELAADFAQLFLLEGNLSAIPYASAYLSGEA
                     LAKNLAEMDRLLGEFGLQVNRETKEPSDHLCVYLTLFDRLLAEKPLETQQQFLQNQLM
                     SWLPAWFAKAKTVKTNTGVYPALLRLFEQVLLKEQR"
     gene            4677..5366
                     /locus_tag="PXE44_002217"
     CDS             4677..5366
                     /locus_tag="PXE44_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL5589685.1"
                     /translation="MQNNKTTYLNWYDLCVLTLILFGEAIYTSTQQYFITTEMQGNFE
                     ENLTFSNEQNYWALLTQLALLLIAMGYLKWRNFDFSRWVIRPSWLAFFSGIGLFILLS
                     LAMDLYFETLYGVYNLVYPEPFWAVLREVNISTVLYAILNGFYEEIFFLGICLAVKPE
                     YLKWAVIYSLIIRVSFHTYQGMESALGLGIWLGLIIYLLYSRSKQKNLFPFWVAHSIA
                     DILGLSLLAYF"
BASE COUNT         1567 a         1187 c         1322 g         1389 t
ORIGIN      
        1 tatttttgat tgatatcaag gcaaattgtt tctgaagata aaataattaa cacttattag
       61 ttaataaaaa aggagttgaa atgcgttcag aactattaaa aacgcttttg ctatcagggc
      121 tgattttacc gctattgatg gcgtttgccg taggagctta cggctttatt gtgtggatgt
      181 tacaactgtt tgttattggt ttaccgggag cgtagaaaat gttaggttca atcaaagcat
      241 tatttactcg gcctgcaacc aaaatcgggt tgggagtgtt agttggtatc ggttttattg
      301 ccggagcaat tagctggcat caatttaata aagcgatgga tcataccagc actgaagagt
      361 tttgcgtgag ttgccactca atgcagcagc cgttagagga gcttaaaaaa accgcccact
      421 ggtcgaataa aagcggggta acggcaacct gttcaagctg ccacttaccg cacgattata
      481 ccgaaaaata tgcccgcaaa gtgcaggcaa gccgtgaggc gtgggcagaa ttatccggca
      541 aatataaaga agaaggctca tttgaaaaac accgcttaga aatggcagag cgtgagtggg
      601 caagatttgc cgccaatggt tcaaaagagt gtaaggcttg ccacagctat gagcgaatga
      661 attttgataa aatgggcgaa gcagctcgta aggcaatgaa accggctgcg gagaaagatc
      721 aaagttgtat ggattgccac aaaggcattg ctcaccactt accgaaagcc gatgaaaatg
      781 cactgaaatc gaagtttgat cagtttgtga ataaatcccc aaaccaaggc aaatcttact
      841 acaccaaagg gaatgccgaa ttatttctag acgaaggttt aagccaagct atcgggcaat
      901 tagaaaccgc cgtgccggtg gcatttgtgc aagcgggcaa aaatgcggat ttggttgagc
      961 tgacaatgtg gcgtaaagag aaaggtttcg gtcgcatttg gtatcacgat ttcgggaaaa
     1021 acattaccga tgcggttcta accaaagagt ttatggcaaa tcagccgaaa tttgaggtgt
     1081 tggaaaccaa agaagatccg attaccggct tgaattggca aaaagtgaaa ctacaagcgt
     1141 ggattggcaa agctcaatta ttagacgatg ttaccccaat ctgggcgaat gcagaaaacc
     1201 aatacaaaac ccaatgtagc acttgccaca gacagcctga tgtcgcccac ttcgattcca
     1261 acagctggat cggcttattc aacggaatgg tcggctttac caatatggat aaacagaccg
     1321 gcaaagaggt gctacgttat ttacaaatgc acgcctccga tagtgaagaa gcgaagcatt
     1381 aataatatta ctcccctctt tactaaagag gggaattaat cgagcgttga ataacattct
     1441 ctgtactagc tcacgttttt acaaataaag aaggacaaga tatgcaacaa tctcgccgtc
     1501 aatttttaaa aaatatgtct gtgatggctg ccgctgtcac aatgccgaat ttcctcgtgc
     1561 ctcgcaatgt gtttgcgagt gaagacctca gtcaatggaa agtttccggc tctcactggg
     1621 gggcaatgcg tgccaaaatt gagaacggac gagtggcaga aatcaaaccg ttcgagtttg
     1681 ataaacaccc gaccgaaatg ctcaacggca ttaaaggctt aatttacagc gattcccgta
     1741 tccgctaccc gatggtgcgt ttagattggc tcaaaaaccg ccataacagc aacaccgcac
     1801 agcgtggcga taaccgcttt gtgcgggtaa cgtgggacga agccctcgac ctgttctacc
     1861 aagagttaga acgggtacaa aaagagtacg gcccgtgggc gttacacacc gccaatgtgg
     1921 gctggcgttc aaccggtcaa ttccacagtt gcggtaacca tatgatccga gcgattgcga
     1981 tgcacggtaa cagtgtcggc acggcaggcg actattctac cggtgcaggg cagaccattc
     2041 tgccgtatgt gctgggttca accgaagtct attcgcaggg cacgtcttgg gaaatcatct
     2101 taaaagagag cgaaaacatc attttctggg caagcgaccc ggtgaagaac ctgcaagtgg
     2161 gttggaactg cgaaacccac gaagcctatg cctacttgga aaaactgaaa gagaaagtgg
     2221 cagcaaaagg cgtgaatgtg atctgtgttg atccggtgaa aagcaaaacc cagaatttct
     2281 taggttgcga acagcagtac atcaatccac aaaccgatgt gccgtttatg ttggcacttg
     2341 cccacacgct ctacaccgag aacctgtacg acaaaaaatt cttggatatg tacacggtcg
     2401 gctttgaaaa attcctgccg tatctgttag gcgaaaccga agacaaagtg gcgaaaaccc
     2461 cggaatgggc ggctgaaatt tgtggtattt ccgccgacaa aatccgtgaa tttgcccgaa
     2521 tgcttgccgg caaacgcacc caatttattt tcggctgggc aattcaacgc caacaacacg
     2581 gcgaacaacc gtactggatg ggagcggtgc tagcctcaat gttagggcaa atcggcttgg
     2641 aaggcggcgg catcagctat gctcaccatt acagctctat cggtattcct gaatccggtg
     2701 cggcaatgcc gggggcgttc ccgctcaata ttgatgaggg gcaaaccccg aaatacgaca
     2761 acaaagatta caaaggctac agcgacacca ttccggtggc tcgtatgacc gacagcttgc
     2821 tccacgccgg cgaaaccatt gattacaacg gcaaaaaagt gacttacgct ccgttcaaaa
     2881 tggcgatttt caccggctgt aaccaatggc accgccattc cgagcggaac aaaatgaaac
     2941 aggcgttcca aaaactggaa accattgttt cgatcaacta cagttggacg gcaacctgcc
     3001 gcttctccga catcgtgctg ccggcctgta ccccatttga acgcaacgat attgacgcct
     3061 acggctcata cagcaaccgt ggcgtgattg caatgcacaa gctggttgat ccactctacg
     3121 actctcgctc cgactttgag attttccgag atctctgccg ccgtttcggt aaagagaaag
     3181 agtacagccg tggaatggac gaaatgcagt gggtagaaaa actctacaaa gattgtcgcc
     3241 gtgaaaacag aggtaaattt gaaatgccgg agtttgctga attttggcaa aaaggctatg
     3301 tgctgttccc agagggcaaa ccttgggttc gccacgccga tttccgtgaa gatccggaat
     3361 tgcacgcact tggcacaccg tccggcttta tcgaaattta cagcaacaaa atcgcctctt
     3421 tcggctatga cgattgcaaa ggtcacccaa tgtggtttga gaaagccgaa cgttcacacg
     3481 gcggtaaaaa ttccgacaaa ttcccgtact ggttgcaatc agttcacccg gacaaacgct
     3541 tacactctca actttgtgaa tcgaaagaac tgcgtgaaac ttacagcgtg caagggcgtg
     3601 aaccgcttta cattaaccca caagatgccg ccaaacacgg cattcaagac ggagatttag
     3661 tgcgagtgtt taacgaccgt gggcaagcga ttgtgggggc ggtgctttcc gacaacttcc
     3721 cgaccggcgt agtgcgttta caagaagggg cttggtattc accccttgac gaaagcgtgg
     3781 gggcgattga cacctatggc gacccgaaca caatgaccct cgacatcggc acgtccaagt
     3841 tggcacaagc ggtttccgcc aacacttgct tggtgaacat cgagaaattt atcggcaatg
     3901 ccccggctcc gaatggcttt aacggtgcga ttgaggtaac ggcttaatga cgttggcaat
     3961 cagtcgtgaa gaacggcaat ttatctatgg ctggtttaac gcaatgctcg cccgagaact
     4021 ttcggacgag cagttaaatg ccttacaagc gggcgaattt gacgattttt ttgcatacct
     4081 cagcgaattg ggttttgccg aacaggttgc aaaatttcaa gcagaagtga ccgcttgtag
     4141 gcagctggaa tttcctcgtc tagaactggc ggctgacttt gcccaactgt ttttattgga
     4201 aggcaatctc agtgccattc cttacgcctc cgcctattta agcggcgaag cgttagcgaa
     4261 aaatttggcg gaaatggaca gactgctggg cgaatttggg ctgcaagtta atcgagaaac
     4321 caaagagccg agcgatcacc tttgcgttta tctcaccctt tttgaccgct tgcttgctga
     4381 aaaaccgttg gaaacgcaac agcaatttct gcaaaaccaa ctgatgagct ggctccctgc
     4441 gtggttcgcc aaagccaaaa ccgtcaaaac taacaccggc gtttatccgg cgttgctaag
     4501 gttgtttgaa caggtgttgc tgaaggaaca aagataaagt tatttatttg agaatgattt
     4561 gtattgagcg aattgagaaa ggaagttaaa atttcaactt ccttttttat tggctggatt
     4621 tatccacatt attacaagcg gttattttta taaaaaaatt tacaaaggag acattaatgc
     4681 aaaataataa aaccacctac ctaaattggt acgatttatg cgtgctaacc ctgatcctgt
     4741 ttggtgaggc aatttatacc tcaacccagc aatatttcat caccaccgaa atgcaaggta
     4801 attttgaaga aaatttaacc ttttcaaatg aacaaaatta ttgggcatta ctgacgcaac
     4861 ttgcattgtt gctgattgca atgggctatc taaaatggcg gaattttgat ttcagccgtt
     4921 gggtgattcg tccctcttgg ctggcttttt ttagcggtat cgggttgttt atcttgctct
     4981 cgttagcgat ggatctctat tttgaaacat tatatggtgt ttacaaccta gtgtatccgg
     5041 agccattttg ggcagtattg agagaagtga atatttctac cgtgctgtat gcgattttaa
     5101 acggctttta tgaagaaatc tttttcctcg gtatttgctt ggcggttaaa cctgaatatt
     5161 taaaatgggc ggtgatttat tcactgatta ttcgggtgag ttttcatacc tatcaaggaa
     5221 tggaaagtgc gttaggatta ggaatttggc tcggtttaat tatttatttg ctctactcac
     5281 gctcaaagca gaaaaaccta tttccatttt gggtggcaca cagtattgcg gatattttgg
     5341 ggctgagttt gttggcttat ttttagttaa aggtgctaca tttaagcggc ataagttgat
     5401 agagctgatc gtaccagtag cataaaaaca ttattatttg tatggaaagg tgcttttagg
     5461 ttaca
//
LOCUS       DANLZC010000149         5457 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.149, whole genome shotgun sequence.
ACCESSION   DANLZC010000149 DANLZC010000000
VERSION     DANLZC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.149"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            39..2105
                     /gene="glyS"
                     /locus_tag="PXE44_002218"
     CDS             39..2105
                     /gene="glyS"
                     /locus_tag="PXE44_002218"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL5589686.1"
                     /translation="MTTQNFLAEIGTEELPPKALKKLATAFAENVENELNQAGLSFEK
                     VEWFAAPRRLAVKVLSLAESQPSKEVEKRGPAVSAAFDAEGKPTKAAEGWAKGCGITV
                     EQAERIATDKGEWLVHRAVIEGQPTKNLLVDIIAKSLANLPIPKTMRWGDKTEQFVRP
                     VHTVTLLFGAELIEGEILGVKSGRTIRGHRFLGEREFQIENADQYPQLLKDRGSVVAD
                     FNERKALILAKSQEKATALGGVADIEEDLLDEVTSLVEYPNVLTAKFEERFLAVPAEA
                     LVYTMKGDQKYFPIYSKDGKLLPHFIFVSNINPEDPSKIIEGNEKVVRPRLTDAEFFF
                     KTDLKQKLEDQLPRLETVLFQQQLGTLRDKTARIEALAGEIAKQIGADETKAKRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDNLPNSLVAC
                     SVALADKFDTLTGIFGIGQAPKGSADPFALRRAALGSLRIIVEKNLPLDLTDLVAKSA
                     QLFGDKLTNKDVVEDVVDFMLGRFRAWYESEGIAVDVIQAVLARRPTKPADFDARVRA
                     VSHFRTLDSAEALAAANKRVSNILAKADIAIGDIDVSACVEPAEKALAEAVIALKAEV
                     QPLIAQGDYTAVLDKLANLRQPVDAFFDGVMVNAEDQKLRQNRLAILSTLQGLFLQVA
                     DISLLQ"
     gene            complement(2210..3511)
                     /locus_tag="PXE44_002219"
     CDS             complement(2210..3511)
                     /locus_tag="PXE44_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDL5589687.1"
                     /translation="MNIHCEKLVQSILHAVNHEVVPALGCTEPISLALASAIARQYLG
                     HLPERIEAKVSPNLMKNGMGVAVPGTATVGLPMAAAIGAIGGEPDGGLEVLKNITPEQ
                     VKQAKAMLNTDKVSVSIFETEHILYSEATLFHQANRVCVRIAEHHTNVIYIEKNGQTI
                     LSKPCAVNNDNTTEIFASLSAKDIFNFSMQIELEKIRFIEQAAQLNSALSQEGLRADY
                     GLHIGRTLQKQIGLGLISDDLLNRIVIETTSASDARMGGASLPAMSNSGSGNQGITAT
                     MPIVVVARHLNVTDEQRIRALFLSHLMAIYIHSKLPKLSALCAVTTAAMGSCAGIAWL
                     LTGKFENISMAISSMIGDISGIICDGASNSCAMKVSTSVTSSYKSILMSMDNSQVTGN
                     EGIVEHDIDRSINNLCSIASRSMQYTDRQVIEIMVNKPKLK"
     gene            complement(3525..4802)
                     /locus_tag="PXE44_002220"
     CDS             complement(3525..4802)
                     /locus_tag="PXE44_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589688.1"
                     /translation="MDTQKLSPAEFKQATKFDSTDFGWIIMSIGMAIGAGIVFLPVQV
                     GLMGMWVFLLSSIIGYPAMYLFQRLFINTLAESPECKDYPTVISGYLGKNWGIFLGVL
                     YFIMLVIWMFVYSTAITNDSASYLLTFGITNTLLSDNPFYGLVLICILVALSSRGEKL
                     LFKISTFMVLTKLFVVAALGLAMIGMWQLHNIGAMPPFGKLIKDAIITLPFTLTSILF
                     LQTLSPMVISYRAKEQSREVARYKALRAMNIAFAILFITVFFYAVSFTLAMGREEALK
                     AYEQNVSALAIAAQFFPGSWATYVGVALNIFAVMTAFFGVYLGFREATQGIVMNLLLR
                     KFPAEKINPNYVQKGIMLFSILLAWSAIVLNAPVLSFTSICSPIFGLVGCLIPAYLVY
                     KVPALFKYKGLALNIIIITGILLCISPFLTFLE"
     gene            complement(4831..>5457)
                     /locus_tag="PXE44_002221"
     CDS             complement(4831..>5457)
                     /locus_tag="PXE44_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL5589689.1"
                     /translation="IMLSYFSLSEAIAGLLGEFCEVVIHSLNAYEKSVIKIINGHHTN
                     RKIGSPITDKGLQLLKCYQKDQSNTELVYFSRLNNGNLVKSTTHIIIGHEGKPIGLFC
                     INLNLSTPLDKTVSSFMNYDPIPPHYKSENFITHPKELIEETFLKAVTHIEQHTTKHS
                     KLYKKSIIFYLFENGIFELKEAVPIVAEKLSLTSHAIYKHLREFKNNT"
BASE COUNT         1620 a         1099 c         1237 g         1501 t
ORIGIN      
        1 atcaaagaac atttatataa aaattggtaa tttaaacaat gacaacacaa aacttccttg
       61 ccgagatcgg cacagaagag ctgccaccga aggcactcaa aaaattagcg accgcatttg
      121 cggagaatgt agaaaacgaa ttaaaccaag caggtctttc gtttgagaaa gtggaatggt
      181 ttgctgctcc tcgccgtttg gcggtgaagg tgttgagcct tgcggaaagc cagccgagta
      241 aagaggttga gaaacgaggg ccggcggtgt cggcagcgtt tgatgccgaa ggcaagccga
      301 ctaaggcagc ggaaggctgg gcgaagggct gcgggattac cgtagagcaa gctgagcgta
      361 ttgccaccga caaaggcgaa tggttggtgc atcgtgccgt gattgaaggt cagccgacta
      421 aaaatctgtt ggtcgatatt atcgccaaat cgctggcgaa cttgccgatt ccaaaaacga
      481 tgcgttgggg cgataaaacc gagcagttcg tccgccctgt gcataccgtg acacttttat
      541 tcggggcgga gttgatcgaa ggtgagatct taggcgtgaa aagcggccgt actattcgtg
      601 gtcaccgttt cttaggtgaa cgtgagttcc aaattgagaa cgccgatcaa tatccgcaac
      661 tgttgaaaga tcgtggatct gtggtagcag atttcaatga gcgtaaagcc ttaattcttg
      721 caaaatcaca ggaaaaagca accgctttgg gtggcgttgc cgatattgaa gaagatttgc
      781 tggacgaagt gacctcgttg gtggaatacc cgaacgtact caccgccaag tttgaagagc
      841 gtttccttgc tgtgccggcg gaagccttag tttacaccat gaaaggcgac caaaaatact
      901 tcccgattta ttcaaaagac ggcaaattgt taccgcactt tatttttgtg tcgaacatca
      961 atccggaaga cccaagcaaa atcatcgagg gtaacgaaaa agtggttcgc ccacgtttga
     1021 ccgatgcgga gttcttcttc aaaaccgact taaaacagaa gttggaagac caactgccac
     1081 gccttgaaac cgtgttgttc cagcaacagc ttggcacatt gcgtgataaa accgctcgca
     1141 ttgaagcgtt ggcaggtgaa attgcgaagc aaatcggggc agatgaaacc aaagcgaaac
     1201 gtgcaggtct cctctcaaaa tgtgacctga tgaccaatat ggtgtttgaa tttactgata
     1261 cccaaggcgt gatgggtatg cactatgccc gccacgacgg tgaagatgaa gaagtggcgg
     1321 tggcgttaaa cgagcagtat atgccacgtt ttgctggcga taacttgcca aattcattgg
     1381 tggcttgctc ggtggcgttg gcggacaaat ttgacacgct aacagggatt ttcggcattg
     1441 gtcaagcccc gaaaggcagt gccgacccgt tcgccctacg ccgtgcggca cttggctcgt
     1501 tgcggattat tgtggagaaa aatttaccgc ttgatttaac cgatttagtg gcgaaatctg
     1561 ctcaattatt tggcgataaa ctcaccaaca aagacgtggt tgaggacgtg gtggacttta
     1621 tgctcggccg cttccgtgcg tggtatgaga gcgaaggcat tgcggtggac gtgatccaag
     1681 ccgttctcgc ccgccgtccg accaagcctg ccgacttcga tgcccgtgtg cgtgccgtgt
     1741 ctcacttccg cacccttgac agtgccgaag ccttagccgc agcgaataaa cgggtgagta
     1801 acattcttgc caaagccgat attgcaattg gcgacattga tgtttcagct tgcgtagagc
     1861 cagcagaaaa agcattagcc gaggcggtta ttgcgttgaa agccgaagtt caaccgctga
     1921 ttgcacaggg cgactacacc gctgtgctgg ataaattagc gaacctccgc caaccggttg
     1981 atgccttctt tgacggagta atggtaaatg cagaagacca aaaactgcgt caaaaccgcc
     2041 tcgccatttt aagcacgcta caaggcttgt tcctgcaagt ggcggatatt tcgctgttgc
     2101 aataacaata agtttgcaat aattgcaaaa ttttaatgaa aatagaccgc ttgcagtaat
     2161 tgtgatctgc aagcggtctt ttttgcttga aattttacca aaggcgggat tatttaagtt
     2221 ttggtttatt caccataatc tcgatgactt ggcggtctgt gtattgcata ctacgagagg
     2281 caatgctgca taagttatta attgaacgat caatatcgtg ttcgacaatt ccttcgttgc
     2341 ctgttacttg gctattatcc atagacatca gaatggattt atagcttgat gttacgctgg
     2401 tggagacttt catcgcacaa ctgtttgaag caccatcaca gataataccg ctaatatcgc
     2461 cgatcatact actaattgcc atactgatat tctcaaattt ccctgtgagc agccacgcta
     2521 tgccggcaca gcttcccata gccgcggttg ttacggcaca gagtgcagat aatttcggta
     2581 acttgctgtg aatataaatt gccatgaggt gtgataaaaa gagtgctcta atgcgttgct
     2641 catcagtgac atttaaatgg cgggcaacga caacaatcgg catagttgct gtaattcctt
     2701 gattacctga acccgaattg ctcatagcag gtaaacttgc accgcccatt ctagcatcag
     2761 aggcagaggt ggtttctata acaatgcgat taagcaaatc atcactgatt agacctagac
     2821 caatctgttt ttgcaatgtc cttccgatat gtagcccata atcggcacgt aatccttctt
     2881 gtgagagtgc ggaatttaat tgtgctgctt gctcaataaa gcgaattttt tccaattcga
     2941 tttgcataga aaaattaaat atatcttttg ccgaaagtga agcaaagatc tcagtagtat
     3001 tatcgttatt gacggcacaa ggtttgctta aaatagtttg cccgtttttt tcaatgtaaa
     3061 tgacattggt atggtgttca gcaatacgaa cacatactcg attcgcttgg tgaaatagtg
     3121 ttgcttcaga atatagaata tgttctgttt cgaaaataga gacagacact ttatcggtat
     3181 ttaacatagc tttagcttgc ttaacctgtt ctggcgtaat attttttaat acttctaatc
     3241 caccatcagg ctcccctccg attgctccaa tagctgccgc cataggcaat ccgacggttg
     3301 cagttcccgg tactgctact cccatcccat ttttcatcaa gttaggggaa acttttgcct
     3361 caatacgttc tggtaaatga cctaaatatt ggcgtgcaat ggcggatgca agggcgagag
     3421 aaataggttc tgtacagcct aatgcaggga caacttcatg gttgactgca tgtaaaatgg
     3481 attgtactaa tttttcgcag tgaatattca tagataaaac cttattactc taaaaatgtg
     3541 aggaatggag aaatacataa taaaatgccg gtaatgataa taatattgag tgctagccct
     3601 ttatatttga ataatgcagg cactttatag actaaatagg ccggaatcaa gcacccaact
     3661 aagccaaaaa taggactaca gattgaggta aagcttaaaa ccggtgcatt taatacaatc
     3721 gcactccaag ccagcagaat ggaaaataac ataatccctt tctgcacata attaggattg
     3781 attttttcag ccgggaattt tcttaataat aggttcatca caatgccttg agtggcttct
     3841 ctaaaaccta aatatacccc gaaaaaggct gtcataacgg caaaaatatt taatgcaaca
     3901 cctacatagg ttgcccaact accagggaaa aattgggctg caattgctaa tgcggaaaca
     3961 ttttgctcgt atgctttgag cgcttcttct ctccccattg caagtgtaaa agagacagcg
     4021 taaaaaaaaa cggtaataaa taagatagca aatgcaatat tcatcgctcg tagggcttta
     4081 tagcgcgcaa cttcacgaga ctgttcttta gcacggtaag aaattaccat tgggctgaga
     4141 gtttgcaaga agagtataga ggttagggtg aaaggaaggg taatgatggc atcttttata
     4201 agtttgccga aaggaggcat tgcaccgata ttatggagtt gccacattcc aatcattgca
     4261 agccctaagg cggcaacgac aaatagctta gttaatacca taaatgtaga tattttaaac
     4321 agtagctttt cgcctcgaga agataaggca actaatatac agattagcac taagccataa
     4381 aatggattat cagagagtag tgtattggta atgccgaagg taagaagata ggaggcactg
     4441 tcgtttgtaa ttgcggtaga ataaacaaac atccaaatca ctaacataat aaaataaagt
     4501 acacctaaaa agattcccca gtttttgccc aggtagccac taataacggt ggggtaatct
     4561 ttgcattccg gagattctgc taaagtatta atgaacagac gctgaaaaag gtacatcgcc
     4621 gggtatccga taatagaaga aagtagaaaa acccacatgc ccataagtcc tacttgtacc
     4681 ggtaaaaaga cgatgcctgc cccgatagcc ataccaatac tcataatgat ccagccgaag
     4741 tcggtagaat caaattttgt tgcttgttta aattcggcag gcgagagttt ttgagtatcc
     4801 atagagtctc cttaaatcgt aacagtataa ttaagtgtta tttttgaatt ctcttaaatg
     4861 tttataaatt gcgtggctgg ttagggagag tttttctgcc acaataggta ctgcctcttt
     4921 tagctcaaag atgccatttt caaacaaata gaagataatg gatttcttat agagttttga
     4981 atgtttggta gtgtgttgct caatgtgagt aactgctttt agaaaggttt cttcaataag
     5041 ttcttttggg tgagtaataa agttttcact tttataatga ggcggaatag gatcgtaatt
     5101 cataaaagag ctaacggttt tatctaatgg tgtcgataaa tttaaattaa tacaaaataa
     5161 tccgataggt ttgccctcat gcccgataat aatatgcgtg gtggacttaa ctaagttacc
     5221 gttatttaac cgtgaaaagt aaaccagctc agtattagat tgatcttttt ggtaacattt
     5281 tagtaactgt aatcctttat ctgtaatagg cgaacctatt tttcggttag tatgatgtcc
     5341 gttaatgatt ttaataacgg atttttcata ggcattcaat gaatgaataa ccacctcaca
     5401 gaattctcct aataaccctg ctattgcttc acttaatgag aaataagaca gcataat
//
LOCUS       DANLZC010000150         5444 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.150, whole genome shotgun sequence.
ACCESSION   DANLZC010000150 DANLZC010000000
VERSION     DANLZC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.150"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            80..1270
                     /locus_tag="PXE44_002222"
     CDS             80..1270
                     /locus_tag="PXE44_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HDL5589690.1"
                     /translation="MFSHIQAAPADSILGLGEAFKADSREGKINLGIGVYMTDEGKTP
                     IVKAVKEAEKRLLETENSKNYLTIDGVQAFNAATQALLFGEGAEVITSGRAKTAQSLG
                     GTGALRIAAEFIKRHTNAKNVWISTPTWPNHNGIFDAVGVNVKGYRYYNKETNGLDWE
                     NLIADLSQAEAGDVVLLHGCCHNPTGIDPTPAQWEQLAALSAEKGWLPLFDFAYQGFA
                     NGLEEDAYGLRAFVKNNRELLVASSFSKNFGLYNERVGAFTLIADNADDANRAFTQIK
                     SIIRVLYSNPSAHGASAVAVALADPDLKAQWIAELDEMRNRIKEMRSQLVQKLKEKGA
                     NKDFSFITEQNGMFSFSGLTPEQVDKLKDEFAIYAVRSGRINVAGITSKNIDALADAI
                     VKVL"
     gene            complement(1377..2678)
                     /gene="folC"
                     /locus_tag="PXE44_002223"
     CDS             complement(1377..2678)
                     /gene="folC"
                     /locus_tag="PXE44_002223"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL5589691.1"
                     /translation="MNTLSIPKATDSLETWLSYLEQAHSKPIDMGLDRIRKVATELDL
                     LKPAPYVITVAGTNGKGSTCRLLEVTLLKAGFRVGVYSSPHLIHYNERVRINGEVLPD
                     ADHVQAFAKINAKKTASLTYFEFSTLSAFELFKQNQLDIVILEVGLGGRLDATNIIDP
                     SLAVIISIDIDHVAFLGDNREDIGREKAGIFRENIPVVIGEPDCPHSIKQIADSLNCH
                     AFYRGKDWQFEIKNNLFHWYSEEKSFTDLPIPLIPIPNAGTALAVLMQLPFEIDEKII
                     RQALNEAQMTGRFQTLTDTDFTHFSQPKPAAKVIIDVGHNPHAARYLADKLRELKQPN
                     QKIYAVFSALEDKDLSGIVEPLANIIDEWHCAGLDCWRGQTGEAVLAKLVNVLPNSTA
                     CSYENVAQASRVLFETANENDIVLVFGSFHTVADFMLWLET"
     gene            complement(2688..3584)
                     /gene="accD"
                     /locus_tag="PXE44_002224"
     CDS             complement(2688..3584)
                     /gene="accD"
                     /locus_tag="PXE44_002224"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDL5589692.1"
                     /translation="MSWLDRILGRDSSSSSNAKSKVPEGVWTKCTSCEQVLYSEELQR
                     NQQVCPKCDHHMRIDARTRLLNLLDKDSAVEIAADLEPEDVLKFKDLKKYKDRLTAAQ
                     KQTGEKDSFIVLAGTLHNMPVVTASFNFEFMGGSMGSVVGAKFVKAAEKALAEKIPFI
                     CFSASGGARMQEALFSLMQMAKTSAILAKMKEQGVPFISVLTDPTLGGVSASLAMLGD
                     VNIAEPKALIGFAGPRVIEQTVREKLPEGFQRAEFLLEHGAVDMIVHRKEMRDTLSRL
                     CAKLTNQPTPFKVAELVVENAE"
     gene            3893..4675
                     /gene="mazG"
                     /locus_tag="PXE44_002225"
                     /pseudo
     CDS             3893..4675
                     /gene="mazG"
                     /locus_tag="PXE44_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
     gene            4743..>5444
                     /locus_tag="PXE44_002226"
     CDS             4743..>5444
                     /locus_tag="PXE44_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5589693.1"
                     /translation="MSKILLIDDDIEFTELLTELLSLEGFKMKVVHNGLEGLNELESN
                     QYDLVLLDVMMPVLSGVKTLKRIRQKYSLPVLMLSARDDQIDRILGLELGADDYVPKP
                     FNNRELVARMKAILRRTTSRPASTDHSEQNYTENISKSLQFAGVELHSGRQQAYYQGC
                     DLDLTGTEFALLQMLMRNSGEILSRELLSLKILGKNLTPYDRAIDMHISNLRKKLPER
                     EDGLPWFKTLRGRGYL"
BASE COUNT         1681 a         1062 c         1107 g         1594 t
ORIGIN      
        1 ttttgctctt gctaaatctg caatttaatc gtataaattc actgtttatt tttcattttg
       61 atgaggaact ccaatgtcaa tgtttagtca cattcaagct gctccggcag actcaatttt
      121 aggcttaggt gaagcgttta aagcagatag ccgtgaaggt aaaatcaacc ttggtatcgg
      181 tgtttatatg accgatgaag gcaaaacccc gattgtcaaa gcggtaaaag aagcagaaaa
      241 acgtttatta gagacggaaa acagtaaaaa ttatttaacc attgacggtg ttcaagcatt
      301 caatgcggca acgcaagcgt tattatttgg cgaaggggct gaagtgatta cctcaggtcg
      361 tgctaaaact gcacagagtt taggtgggac aggggcatta cgcattgcgg cggaatttat
      421 caaacgtcat acgaatgcga aaaatgtatg gatttcaacc ccgacttggc caaaccataa
      481 cggcattttt gatgcagtag gcgtgaatgt aaaaggctat cgctactaca ataaagagac
      541 taacggttta gattgggaaa atttaattgc cgatttaagc caagcggaag ccggagatgt
      601 ggtgttattg cacggttgct gccacaaccc gaccggaatt gacccaacac ctgcacaatg
      661 ggaacagtta gcagcattat ctgccgaaaa aggctggttg ccgttatttg actttgctta
      721 tcaaggcttt gcaaacggtt tagaggaaga tgcttacggt ttacgagctt ttgtgaaaaa
      781 taaccgtgaa ttgttggtcg caagctcatt ctcgaaaaac tttgggttat ataacgagcg
      841 tgtcggtgca tttaccctaa ttgcagataa tgctgatgat gccaaccgtg ctttcactca
      901 aatcaaatcg attattcgtg tactttactc taacccatct gctcacggtg caagtgcagt
      961 agcagttgca ttggctgatc ctgaccttaa agcacaatgg attgcggaat tagatgaaat
     1021 gcgtaaccgt attaaagaaa tgcgtagcca attagtgcag aaattaaaag aaaaaggggc
     1081 aaacaaagac ttctctttca ttactgagca aaatggtatg ttctcattca gtggcttaac
     1141 accggagcaa gtggataagt taaaagatga gtttgcaatt tatgctgtgc gttcaggcag
     1201 aatcaatgta gcaggcatta ccagcaaaaa cattgatgct cttgccgatg cgattgtgaa
     1261 agtgctttaa ttaaacctta aagatatata gaggcataag cctgagtgct tgtgcctctt
     1321 ttttatcttt taggtacaag tgtggacact tgcaccaacg ttgattattc gtagaactag
     1381 gtttccaacc aaagcataaa atctgctacc gtatgaaatg agccgaacac cagcacaata
     1441 tcattttcgt tggctgtttc aaacaatact cggcttgcct gtgccacatt ttcatagcta
     1501 caagcggttg aatttggcaa aacgtttacc aatttcgcta acaccgcctc gcctgtttgt
     1561 ccacgccaac aatctagccc tgcacaatgc cattcatcaa taatattagc taatggctcg
     1621 acaataccgg ataaatcctt atcttctaac gcactaaaca ccgcatagat tttttgattt
     1681 ggttgcttta actcccgcag tttgtcagct aaataacgtg ctgcgtgtgg gttatgccct
     1741 acatcaataa ttacttttgc agccggttta ggttgagaaa aatgagtaaa atcggtatcc
     1801 gtcagggttt gaaaacgccc tgtcatttga gcttcattta aggcttgtcg aataatcttt
     1861 tcatcaattt caaatggcag ttgcattaat actgctaatg cggtgccggc gtttggaatc
     1921 ggaataagcg ggattggtaa atcggtaaag gatttttctt cagaatacca atgaaacaga
     1981 ttattcttaa tttcaaactg ccagtcttta ccccggtaaa aagcgtgaca atttaacgaa
     2041 tcagcaatct gtttaatcga atgagggcaa tcaggctcgc cgatgacaac cggaatattt
     2101 tcacggaaaa tgccggcttt ctcacgcccg atgtcttcac ggttatcgcc taaaaatgca
     2161 acgtggtcga tatcaatcga gataatcacg gcaaggcttg gatcaataat attagtggca
     2221 tctaatcgcc cacctaatcc gacttctaaa atcacaatat cgagctgatt ttgtttgaat
     2281 agctcaaatg cagacagcgt actaaactca aaataagtga gtgaagcggt cttttttgca
     2341 ttaatttttg caaatgcttg gacgtggtcg gcatcaggta atacttcgcc gttaattcgc
     2401 acccgttcat tatagtgaat taagtgcggt gaagagtaaa caccaacccg aaatccggct
     2461 tttaataagg taacttctaa caaacggcag gtcgaaccct tgccgttagt accggcaacg
     2521 gtaatcacat aaggggcagg cttgagtaaa tccagctctg ttgcgacctt acgaatacga
     2581 tctaagccca tatcaatcgg tttagaatgg gcttgttcta aataggaaag ccacgtttcc
     2641 aaagaatccg tggctttagg tatgcttaat gtattcattc agatatttta ttctgcgttt
     2701 tctacaacca attccgccac tttaaacggc gttggttgat tggttaattt tgcacaaaga
     2761 cgggaaaggg tatcacgcat ctctttacga tgcacgatca tatcaactgc cccgtgttct
     2821 aataagaact cggcacgttg gaaaccttcc ggcagttttt cacgtacggt ttgctcaatt
     2881 acacgaggac cagcaaagcc gattagggct tttggctcag caatattcac atcgcccagc
     2941 attgccaaac ttgcggaaac cccacctaaa gttgggtcgg ttaatacaga aataaacggt
     3001 acgccctgct ctttcatttt ggctaaaatc gcactggttt ttgccatttg cattaaagag
     3061 aataacgcct cttgcatacg agccccgccc gatgccgaaa aacagataaa cggaattttt
     3121 tcagctaaag ctttttccgc agctttcaca aattttgcac ctactacaga ccccattgaa
     3181 ccgcccataa actcaaagtt aaaggaggcc gtaaccaccg gcatattatg caatgtaccg
     3241 gcaagcacga taaaagaatc tttctcaccg gtttgttttt gcgcggcagt taaacgatct
     3301 ttatatttct ttaaatcttt aaatttcaac acatcttccg gctctaagtc ggcagcgatt
     3361 tcgaccgcag aatctttatc taacaaattc aataaacggg tgcgggcatc aatacgcata
     3421 tgatgatcgc attttggaca aacctgttgg ttgcgttgta attcttcgct atagagaact
     3481 tgttcacaac ttgtgcattt tgtccataca ccttccggca cttttgattt tgcattgctt
     3541 gatgatgaag agtctctacc taaaattctg tctaaccagc tcattattat tccttttgga
     3601 ttgagttgtt gggttcacta aatggttgct attaaagcat aatttatcgg tttttgatat
     3661 gttttcttga catatcagat cactaactat gtgtataagt tcgataaaaa aacgcaatca
     3721 gaagaatgat taacatcacc acaatttgaa ttaagcgttt tttggttaat ttttccgctg
     3781 ccattgctct gctcatactt aataaatttc cgcattataa gcgaaaaagc acgggctatg
     3841 tagaatttgc aaaattttga tcaaaaataa ccgcttgtaa aaaggaaata ctatggcaac
     3901 cattgaacaa tttcttgagg ttgtaaacca acttcgtcac cctgaacacg gctgcccgtg
     3961 ggatctcaaa cagaattttg acacgatgtt ccccacttat tggaagaaac ctacgaagta
     4021 gcggaagcga ttcacaccca agatcgcact gccttgcgtg aagagttagg cgatttatta
     4081 ctgcaagtgg tatttttaag ccaattagcg aaagaggaag gcagtttcac ttttgatgat
     4141 gtagtaacgg atattcacga taaactgatt taccgccacc ctcacgtttt tggtgatgtg
     4201 aaagctacta acagcgaaga ggccttacaa agttgggaag cacaaaaagc gaatgatgca
     4261 aaacaccgta gccaagattc tatttttaga tgatttacct tttgctttgc ctgctcttac
     4321 ccgtgcaaat aagctacaaa aacggtgtgc aaaagttgga ttcgattggg ataaccctca
     4381 cgatgtgctg gcaaaagtcg aagaagaact cgatgaagtc aaagcagaaa tcgcacaata
     4441 tccggctcgc tccatcgagc ttgctgaaga attaggcgat ctcttgtttg cgattgtaaa
     4501 tttatgccgc cattatcata ccgatgccga ggaaatttta cgcaatgcaa atctaaaatt
     4561 tgaacatcgc tttcgaaaag ttgaaaaatc tgtgaaaaaa tcgggaagaa ctgtaaaaga
     4621 atgtaaatta gaagaactag atgtaatttg gcacaaaata aaagtggtaa aataaaaagc
     4681 accaattgtt atactaattt ttacctttgt aaagttagtt ttaattttat agggaatatt
     4741 ttatgtcaaa aatcttatta atagatgatg atattgagtt tactgagcta ctaactgaat
     4801 tactttcatt agaaggattt aaaatgaaag ttgtgcataa tggtttagaa ggcttaaatg
     4861 agttagagtc taatcaatat gatttagtat tactagatgt gatgatgccg gtattaagtg
     4921 gcgtgaaaac attaaaacgt atacgacaaa aatactcatt acccgtttta atgttaagtg
     4981 caagagatga tcaaattgat agaatcttag gtttagaatt aggggctgac gattatgtac
     5041 cgaaaccttt taataatcgt gaactagtcg ctagaatgaa agcgatactt cgaagaacaa
     5101 cctctcgccc agcttcaaca gaccattctg aacagaatta tactgaaaac atctctaaat
     5161 cattacaatt tgccggagtt gaattacatt caggacgtca gcaggcttat tatcaaggat
     5221 gtgatcttga tttaacgggg actgaatttg cattattaca aatgttaatg cgaaactcag
     5281 gggagatttt gtccagagaa ttattgagtt taaaaatttt agggaaaaat ttaacgcctt
     5341 atgatcgtgc aattgatatg catatttcta atcttcgtaa aaaattaccc gaacgggaag
     5401 atggattacc ttggtttaaa accttaagag gtcggggtta ctta
//
LOCUS       DANLZC010000151         5302 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.151, whole genome shotgun sequence.
ACCESSION   DANLZC010000151 DANLZC010000000
VERSION     DANLZC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.151"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(18..1301)
                     /locus_tag="PXE44_002227"
     CDS             complement(18..1301)
                     /locus_tag="PXE44_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5589694.1"
                     /translation="MTACHEPKRSIWQQIFTKNMLLCVYTGFCSGLPLFVLIQLMPAW
                     LTSANLDIKTIAAFTLTSLPYTWKFLWAALLDRYFPPFLGRRKSWIFISQIGLLVILA
                     SFGLFNPVQDISIIVTLSVLLAFLSATQDIVVDAFRREILSDNELGLGNSIHVNAYRI
                     AGLIPGGLSLFLADHYAWDTVFLITAAFLVPCLFVTLIIAAEPNHKPIDRSKPFYMAF
                     VDPFKEFFTRKGVAGAIGLILFIFLYKLGDSLATTLQTKFILDMGFTKSHIAGIVKIT
                     SLWCSIGGGIIGGVAMLKLGVNRALWLFGFVQLITILGFAYLASFEHFPTESIGAAEL
                     WKLGLVMAGEYLGVGLGTAAFVAFMARETNPAYTAMQLAIFTSLAALPSKMLGAYTGH
                     LVEMMGYYQFFWLCFFIGIPGMLMLFKVAPWGQAE"
     gene            1467..5183
                     /gene="metH"
                     /locus_tag="PXE44_002228"
     CDS             1467..5183
                     /gene="metH"
                     /locus_tag="PXE44_002228"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL5589695.1"
                     /translation="MNTQHRTELLQNALTERILILDGAMGTMIQKYKLTESDFRGERF
                     KNSTIDLKGNNDLLTLTQPLLISAIHEKYLAAGADIIETNTFSATTIAQADYELESIA
                     YELNLVGAKLARLAADKFSIPEKPRFVAGILGPTNRTCSISPDVNDPGFRNITFMQLV
                     EAYAEATRGLIEGGADLIMIETIFDTLNAKAAVFAIETVFDELGVKLPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAKPLTFGLNCALGPKELRPYVEMMSKISETFVSVHPNAG
                     LPNAFGGYDLGAEEMATYIKEWAELGWLNIVGGCCGTTPEHIQAFAEATKGVAPRKLP
                     EIPTAMRLSGLEPLTIDDNSLFVNVGERNNVTGSAKFKKLIKEEKFAEAIEIAISQVE
                     NGAQVIDVNMDEALLDSKKCMTRFLNILATEPEAAKVPIMIDSSKWEVIEAGLQTVQG
                     KPIVNSISLKEGEEKFIHQAKLCRRYGAAVVVMAFDEVGQADTEERKVEICTRAYRIL
                     VDQLGFPPEDIIFDPNIFAIATGIEEHNNYGVDFIQACERIKRDLPHAKISGGVSNVS
                     FSFRGNNVVREAIHAVFLYYAIKAGMDMGIVNAGQLAIYDDLDPELREAIEDAVLNHR
                     HDTTDRLLEISEKYRGVKVESADESAAEWRNLPVAERLKHALVKGITTYIIEDTEEAR
                     QQFASPLEVIEGPLMAGMDVVGDLFGDGKMFLPQVVKSARVMKQSVAYLEPYINATKQ
                     KGSSNGKMVIATVKGDVHDIGKNIVSVVLQCNNFEVIDLGVMVPADVIIETAIREKAD
                     LIGLSGLITPSLDEMEYFLGEMNRLKLDIPVLIGGATTSKEHTAIKLYPKYNYPVVYT
                     ANASRCVTVCATLMNPEAKAEFWEKTKAEYEEIQRKFAVRKPLRNGLSIEEARANRFD
                     GFSGEWADYVPPTPKQTGIIEYKNVPIATLRKFIDWSPFFMLWGLMGGYPDAFDYPEG
                     GEEARRVYNDAQRVLDEFEQNGKLNPSGIMGIFPAVREGDDIAILNEDRTACIGKSYH
                     LRQQSERGKNSKSPYNLCLSDFIADRESGQQDWFGMFAVCAGIEEHYLVESYKAAGDD
                     YNAILLQAVGDRLAEAMAEYLHFELRTKVWGYTVEDEDNGRLIREEYIGIRPAPGYPS
                     CPEHTEKQIIWDLLEVEQRIGMKLTESYAMWPAASVCGWYFTHPTSNYFTLGKIDEDQ
                     VLDYAKRKGWDELTAKKWLSVAMKQDVKSQAENI"
BASE COUNT         1551 a         1225 c         1294 g         1232 t
ORIGIN      
        1 cttattttta tgaatagcta ttcagcttgt ccccaaggag caaccttaaa caacattagc
       61 attcccggaa taccaataaa gaaacagagc cagaagaatt ggtaatagcc catcatttcc
      121 actaaatgcc ctgtgtatgc ccctaacatt ttgcttggta gggcagcaag gctggtgaaa
      181 atggcgagct gcattgcagt gtaagcggga ttagtttcac gagccataaa ggcaacgaaa
      241 gccgctgtgc ctaaacccac gcccagatat tcacctgcca tcactaagcc taatttccaa
      301 agctcggcag ctccaatgct ttcggtaggg aaatgctcaa aactggcaag gtaagcgaag
      361 cctaaaatag taattaattg tacaaatccg aacagccata aggctcggtt aacgcccaat
      421 ttcaacattg ccacgccacc aataataccg ccgccaatgc tgcaccaaag tgaagttatt
      481 ttcacaatgc cggcaatgtg cgatttagta aaacccatat ccaaaataaa tttggtttgt
      541 agcgtagttg caagggaatc gcctaattta tataggaaaa taaatagaat cagcccaatc
      601 gctcctgcca cgcctttacg ggtgaaaaac tctttgaacg gatcgacaaa cgccatataa
      661 aacggcttag aacggtcgat tggcttatga ttcggctcgg cagcgattat tagcgtaacg
      721 aataagcaag gcactaaaaa agcagcggta atcagaaata cggtatccca cgcatagtga
      781 tcggctaaaa ataatgaaag cccacccgga atcagccctg caatacgata agcattaacg
      841 tggatagagt tacctaaccc gagttcgtta tccgataaaa tttcacgccg aaaggcatcc
      901 accacaatgt cttgcgtggc ggaaagaaaa gctaatagca cagaaagagt aacaataatt
      961 gaaatatctt gcactgggtt gaacaagcca aagctggcta aaatcaccaa tagcccgatt
     1021 tgtgaaatga aaatccagct tttgcgtctg cctaaaaaag gtggaaagta gcgatctaac
     1081 aatgcagccc acaggaattt ccacgtgtag ggcaaagagg tcagggtgaa ggcggcaata
     1141 gttttaatat ctaaattggc actggttagc caagccggca ttagctgaat taacacaaac
     1201 aacggtaaac cggaacaaaa gccggtgtaa acacaaagca acatattttt agtaaaaatc
     1261 tgttgccaga tagagcgttt gggttcgtga caagcggtca tatttttcct acattttgca
     1321 aaataaaaag gggaagaact gagccaatat agcataattt taaaggcgac tctaaccctt
     1381 tccatttcgt cacagataag gtttgacagc cttgcgacat atttctatta ttccttttaa
     1441 tcaaactaaa aaactggaag cagagtatga acacgcagca tagaaccgaa ttacttcaaa
     1501 atgcccttac cgaacgcatt cttatcttag acggtgcgat gggaacgatg atccaaaaat
     1561 ataaactaac cgaatctgat ttccgaggtg aacgttttaa aaacagtacc atcgatctta
     1621 aaggcaataa cgacctactc acactcaccc agccactcct gatttctgct attcacgaga
     1681 aatatttagc cgccggtgcg gatattatcg aaactaatac ctttagtgcg acgaccattg
     1741 ctcaagcaga ttatgaactt gaaagtattg cttatgagct gaatcttgtg ggggcaaaac
     1801 ttgcccgtct tgccgccgat aaattctcca tccctgaaaa acctcgtttt gttgccggta
     1861 ttcttgggcc gactaaccgt acttgttcga tttcccctga tgttaatgac cccggtttcc
     1921 gtaacattac ctttatgcaa ctggtggaag cctatgccga ggcaacccgt ggtttgattg
     1981 aaggtggggc ggatctgatt atgatcgaaa ccattttcga taccctgaat gccaaagcgg
     2041 cggtgtttgc aattgaaacg gtgtttgatg aactgggcgt gaaattgccg ataatgatct
     2101 ccggcaccat taccgatgcc tcagggcgaa cgctgtcagg gcagaccacg gaggcgtttt
     2161 ataactcgct ccgccacgcc aaaccgctga cgttcggctt gaactgcgcg ctcgggccga
     2221 aggaactccg cccttatgta gagatgatgt cgaaaatcag tgagactttt gtttcggttc
     2281 acccgaatgc cggcttgcca aacgcttttg gcgggtacga tttaggggcg gaagagatgg
     2341 cgacttacat caaagagtgg gcagaactcg gctggctgaa tattgtcggc ggctgttgtg
     2401 gcaccacgcc ggagcatatc caagcctttg ccgaagcaac caaaggcgtt gcgcctcgta
     2461 aattacctga aattccaacc gctatgcgtt tgtcgggctt agaaccgctg acgattgacg
     2521 ataattcgct gtttgtgaat gtgggcgaac gcaataacgt caccggttcg gcaaaattta
     2581 agaagctgat taaagaagaa aaatttgcgg aagcgattga aattgccatc agccaagtgg
     2641 aaaacggggc gcaggtgatt gacgtgaata tggacgaggc gttgctcgat tccaaaaaat
     2701 gtatgacccg tttcctcaac attcttgcta ccgaacctga ggcggcaaaa gtgccgatta
     2761 tgattgactc ctcgaaatgg gaggtgatcg aggcgggctt gcaaacggtg cagggcaagc
     2821 cgatagtgaa ctccatttca ctcaaagagg gcgaagagaa atttattcac caagccaaac
     2881 tctgccgccg ctatggggct gcggtggtgg tgatggcatt tgacgaagtc gggcaagcgg
     2941 ataccgaaga acgcaaggta gaaatttgta cccgagctta tcggattttg gttgatcagc
     3001 tcggcttccc accggaagac attattttcg acccgaacat ttttgcgatt gccaccggta
     3061 ttgaagaaca caataactac ggcgtggatt ttatccaagc ctgtgagcgg attaaacgag
     3121 acttgccaca cgccaaaatt tccggcgggg tgtcgaatgt ctctttctct ttccgaggca
     3181 acaatgtggt gcgtgaggct attcacgccg tgttcctcta ctatgcgatc aaagccggaa
     3241 tggatatggg gatcgtgaac gcagggcagt tggcgattta tgacgacctc gaccctgaac
     3301 tgcgagaggc gattgaagat gcggtgctga accaccgtca tgacactacc gaccgcctgt
     3361 tggaaatttc cgaaaaatat cggggcgtaa aagtagaaag tgccgatgaa agtgccgccg
     3421 agtggcgaaa tttacctgtg gcagagcgtt taaaacacgc cctcgtaaaa ggcataacga
     3481 cctacattat tgaagatacc gaagaggctc gccagcaatt tgcctctccg ctggaagtga
     3541 ttgaagggcc gctcatggcg ggtatggacg tggtgggcga cctgttcgga gacggcaaaa
     3601 tgttcctgcc gcaagtggtg aaatcggctc gggtaatgaa acagtccgtt gcctatttag
     3661 agccgtacat caacgccacc aagcagaaag gatcgagcaa cggcaaaatg gtgatcgcca
     3721 ccgtgaaagg tgatgtacac gacatcggca aaaacatcgt tagcgtggtg ttgcagtgca
     3781 ataactttga agtgatagac ttaggcgtga tggtgcctgc cgatgtaatt atcgaaactg
     3841 ctattcggga gaaagccgat ctcattgggt taagcggact aattacccca tcgctggacg
     3901 aaatggagta ttttttaggt gaaatgaacc gcttgaagct cgatattccg gtgctgattg
     3961 gcggcgcaac gacctcaaaa gagcatacgg cgatcaaact ttatccgaaa tataactacc
     4021 ccgtagtcta taccgccaac gcttcacgtt gcgtaaccgt ctgtgcgacc ttgatgaacc
     4081 ctgaggcaaa agccgaattt tgggaaaaaa ccaaagccga atacgaagaa attcagcgta
     4141 aatttgccgt tcgcaaacca cttcgcaacg gtttatctat tgaggaggct cgagccaatc
     4201 gttttgacgg atttagtggc gaatgggcag actatgtgcc gccaacgcca aaacagaccg
     4261 gcattatcga gtacaaaaat gtaccgattg ccaccctacg caaatttatc gactggtcgc
     4321 cgttctttat gctctggggc ttaatgggcg gttatcctga tgcctttgat tatccggaag
     4381 gcggcgaaga ggcacgccgt gtctataacg atgcccaacg ggtgctggac gaatttgagc
     4441 aaaacggcaa gctcaaccca agtgggatta tgggcatttt ccctgccgtg cgtgagggcg
     4501 atgatattgc aattttaaac gaagatcgca ccgcttgtat cggcaaatcc taccacctcc
     4561 gccaacaatc cgaacgaggc aaaaacagca aaagcccgta caacctctgt ttaagcgatt
     4621 ttatcgccga ccgagaaagc ggacaacaag actggttcgg aatgttcgcc gtttgtgccg
     4681 gcattgaaga acactattta gttgaaagct acaaagcggc aggcgatgac tacaacgcca
     4741 ttttgctcca agccgttggc gaccgcttgg cagaagcaat ggcggaatac ctacactttg
     4801 agctacgcac caaagtctgg ggctacaccg tggaagacga agacaacgga cgcttaatcc
     4861 gtgaagaata catcggcatc cgccctgcgc ccggctaccc aagctgcccg gaacacaccg
     4921 agaaacagat aatttgggac ttgttagagg tggaacaacg catcggcatg aaactcaccg
     4981 aaagctatgc catgtggccg gcagcaagtg tctgcggctg gtacttcacc caccctacca
     5041 gcaactactt cactctcggc aagattgatg aagatcaggt gttggattac gccaaacgca
     5101 aaggctggga cgaactcacg gcgaagaaat ggttaagtgt ggcgatgaaa caagatgtca
     5161 agtcgcaggc tgaaaacatt taatgtagtg gcgggtttta acccgcctgt tacaagcggt
     5221 tggttttgag ataaaatatg caaaatttat ctctctttta tgcaaagaag ttatcatcga
     5281 gaacaaaaca atgattgaaa aa
//
LOCUS       DANLZC010000152         5244 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.152, whole genome shotgun sequence.
ACCESSION   DANLZC010000152 DANLZC010000000
VERSION     DANLZC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.152"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..518)
                     /locus_tag="PXE44_002229"
     CDS             complement(<1..518)
                     /locus_tag="PXE44_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5589696.1"
                     /translation="MLDSLKEVNQKLDKKADKVGGGTKEDALVTLDTLIASGTVKAET
                     AMSLLPTLQKGAVATGASSEDMAKIAISSMQQFGIKEEDIGRALDMAVAAGQAGSFEL
                     AYMASWLPQQMAAAKQAGLSSIEGFERLLIANQQARVTAGTSDEAGNNLVNLLGKITA
                     KETNERFKNIEY"
     gene            617..868
                     /locus_tag="PXE44_002230"
     CDS             617..868
                     /locus_tag="PXE44_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5589697.1"
                     /translation="MAPTISDGDQMLVDFSKKEARGDDKIYLVQNGESVWVKRVRKVW
                     DYVELISDNESYRPIRITADEAQNLQIIGQVVHIGHSLV"
     gene            complement(1064..1264)
                     /locus_tag="PXE44_002231"
     CDS             complement(1064..1264)
                     /locus_tag="PXE44_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL5589698.1"
                     /translation="MQYVLDLTGYACPLPLLMAKKAMNDLKKGDSLEILLNQQSSLTD
                     FELLAKEQGWLCELQAVENVGY"
     gene            complement(1323..1835)
                     /locus_tag="PXE44_002232"
     CDS             complement(1323..1835)
                     /locus_tag="PXE44_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HDL5589699.1"
                     /translation="MQQLEPQTFASWAEPIDMLYACHSKVKSFCKQLQILPDYLEKNG
                     VNQAVKNDVQQILNYFNISAPLHHEDEEDDFFPELIKVQPQAQAEVDELERQHVHLHK
                     NWDYLSAQLEDLLAGKRENVDRDLIERFVAGYDVHIAIEEPLFELGREHLAQDKLSAM
                     GKVMADRRKL"
     gene            complement(1848..3002)
                     /gene="tgt"
                     /locus_tag="PXE44_002233"
     CDS             complement(1848..3002)
                     /gene="tgt"
                     /locus_tag="PXE44_002233"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL5589700.1"
                     /translation="MKYELKTTSGNARRGRLTFNRPKGEFHVETPAFMPVGTYGTVKG
                     MTPEEVKATGAQILLGNTFHLWLRPGQEVMKMHGDLHDFMQWHGPILTDSGGFQVFSL
                     GKLRKIKEEGVTFQNPISGEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
                     KSMEMSLRWAKRSRDRFDELENPNALFGIIQGGIYEELRKVSVEKLVEIGFDGYAVGG
                     LAVGEPKEDMHRILEYVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNA
                     RNGHLFVTNGIVKIRNAKYRADTTPLDPECDCYTCKNYTKAYLYHLDKCGEILGARLN
                     TIHNLRYYQRLMAEIRQAIEEDRFDDFVVEFYARIGKEVPPLQSEVKEQK"
     gene            complement(3061..3201)
                     /locus_tag="PXE44_002234"
     CDS             complement(3061..3201)
                     /locus_tag="PXE44_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589701.1"
                     /translation="MKKLLLCVGILVLAACSSTGVGGSIGAGGGSNGVGIGLGVGTDF
                     RF"
     gene            3295..3867
                     /locus_tag="PXE44_002235"
     CDS             3295..3867
                     /locus_tag="PXE44_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HDL5589702.1"
                     /translation="MKILRKFAAVCVVSMAMLSANANLLTISEQEINDYLATKLAEKI
                     PLKDKVGIPKLFELDYRLHSLTSQIGRTEEKKVAISGVVDGLLNAKGKKYAASIHLNM
                     DTTPYYDPQKGALFLKDVRLLSWSATPAKYQAELQMFLPVLADGLSSFLNSTPVYTLD
                     ENKTKEAMVKKFGKAIVVENGNIKLETTIF"
     gene            3969..4187
                     /gene="cspD"
                     /locus_tag="PXE44_002236"
     CDS             3969..4187
                     /gene="cspD"
                     /locus_tag="PXE44_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HDL5589703.1"
                     /translation="MDIGIVKWFNNVKGFGFITSDVCEGDIFAHFSEIQAEGYRSLKV
                     GQKVQFELVQGERGASASNITPISEKTL"
     gene            4277..5191
                     /locus_tag="PXE44_002237"
     CDS             4277..5191
                     /locus_tag="PXE44_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5589704.1"
                     /translation="MPSSYQLVLINLLYIFGIGLNIVLLRFLSLHFDPLNNNGIRFLV
                     GGFTLLFFILWRFRTSLSQLIKTPKLIAISLFVGLMMSANMYFWLKGVKITNAVTVSI
                     FGVLAMPFGVIIAAIFFQDERERIKSKAFWFGSILTIIGSLGFIWHGKTLSIGEGFIL
                     GSVFLFLSIIIRNIQNLVVKFARELNVLTLSCVTSLTTSLSSLLLSQQAGKFNELFTA
                     PNWLLFSLMVAGIYAIMIGMVLTFHIIQTQGLITYQVLELLMPVSTAIIAYILLDERI
                     SIVQMGFACIVILGSSIALKLVKYPKHY"
BASE COUNT         1505 a         1105 c          968 g         1666 t
ORIGIN      
        1 ttatattcaa tatttttaaa acgctcattt gtttctttag ctgtaatctt accaagcaga
       61 tttaccagat tattacctgc ctcatcgctt gtaccggcag tgacacgagc ctgttggttt
      121 gcaattaaaa gtcgctcaaa gccctcaata ctactcaaac cggcttgttt tgcagctgcc
      181 atttgttgag gcaaccaact tgccatatat gcaagctcaa aactacctgc ttgcccggct
      241 gcaacagcca tatccaatgc ccgcccaata tcctcttctt tgatgccaaa ttgttgcata
      301 gaggagatcg caatttttgc catatcctcc gagcttgccc ctgttgccac cgcccctttc
      361 tgcaaagtag gtaatagact cattgcggtt tcggctttga ctgtaccgga ggcaatgagc
      421 gtatcaagcg taactaacgc atcctctttt gtgccacccc caaccttatc cgccttttta
      481 tcaagttttt ggtttacttc tttgagcgag tctaaaatat cgtccagctt ttccttaaga
      541 gccttattca gacagcctat taagcctagg cgtaaaagcc cataattgtg gcgtattttg
      601 ggctaatggc acatcaatgg ccccgactat cagcgatggt gatcaaatgt tagtggattt
      661 cagcaaaaaa gaagcccgtg gagacgataa gatttatctt gtgcaaaatg gtgaaagcgt
      721 ttgggtaaaa cgtgtaagaa aagtgtggga ctacgttgaa ttgattagcg ataatgagtc
      781 ctaccgccca atccgaatta cagcagacga agctcaaaac ttacaaatca tcgggcaggt
      841 agtccacatc ggacactctt tagtttaaaa cagatttaaa atagttttaa aaccgatcaa
      901 acctatgcaa aaaatatcgc aaaaactgcc catttttttc taatcctgct cattttgttt
      961 tttgcatagc cccaaccaaa caataacggc gaacccttgt attcacgggg ctcgccgtta
     1021 tttttccgcc caaatttttt tctttctctc tatgcaaaaa agatcagtac cccacatttt
     1081 caaccgcttg tagctcacaa agccaacctt gctctttggc aagcagttca aaatcggtaa
     1141 ggctactctg ctgattgagt aaaatttcca agctatcgcc ctttttaaga tcgttcatcg
     1201 ctttttttgc cattaataac ggcaaggggc aggcatagcc ggttaaatct aaaacatatt
     1261 gcatagcgaa atagggaata aagaaaaagg gcgaatattg aaattcgccc aatcacaaaa
     1321 gattagagtt tacggcggtc tgccatcact ttgcccatag ccgataattt gtcttgagct
     1381 aaatgctctc gcccaagctc aaagagtggc tcttcaatcg caatatgcac atcataacct
     1441 gccacaaaac gctcaattaa gtctctatcc acattttcac gtttacctgc gagcagatct
     1501 tcaagctgtg ccgacaaata gtcccaattt ttatgtaaat gaacgtgctg gcgttccagt
     1561 tcgtctacct ctgcttgagc ttgtggttgc accttaataa gctcagggaa gaaatcatct
     1621 tcttcatctt cgtgatgtaa aggggcagaa atgttgaagt agttcaaaat ctgctgcacg
     1681 tcattcttca ctgcttggtt taccccgttt ttctcaaggt aatccggcaa aatttgcaac
     1741 tgtttacaga aactttttac tttgctgtgg caagcataaa gcatatcaat cggctctgcc
     1801 cagcttgcaa aggtttgtgg ttcaagttgt tgcatataca ctccttgtta tttctgttct
     1861 tttacttcag attgtaaagg tggcacttct ttgccaattc gggcataaaa ttccactaca
     1921 aaatcatcaa aacgatcttc ttcgatggcc tggcgaattt ctgccattag gcgttgatag
     1981 tagcgtaaat tatgaatggt gtttagtctt gcccctaaaa tctcgccaca tttgtctaag
     2041 tgatataaat aggctttggt gtagtttttg caagtgtaac aatcacattc aggatcaagc
     2101 ggtgtggtgt ctgcacgata tttcgcatta cgaattttta caatgccgtt ggtcacaaat
     2161 aaatgtccgt tacgggcatt gcgggttggc attacgcagt caaacatatc aataccacgg
     2221 cgaacccctt cgactaaatc ttctggcttg cctacaccca ttaaatagcg tggtttatct
     2281 gccggaattt gcgggcaaac gtattctaaa atgcggtgca tatcttcttt aggttcacct
     2341 accgccaagc caccgactgc atagccatca aagccgattt caactaattt ttccaccgag
     2401 actttgcgaa gttcttcata aataccacct tgaataatgc caaataacgc attcgggttt
     2461 tcaagttcat caaagcgatc acgagaacgt ttcgcccaac gtagtgacat ttccatcgat
     2521 ttttttgcat aatcaaaggt tgccggataa ggagtacatt catcaaaaat cattacaatg
     2581 tctgatccaa gatcgtattg gatctccatt gatttttccg gcgaaaggaa aattttctca
     2641 ccgctaatcg ggttttggaa agttacgcct tcttctttaa ttttacgcag tttacctaag
     2701 ctaaaaactt ggaaaccgcc ggagtcggtt aaaatagggc cgtgccattg cataaaatcg
     2761 tgcaagtcac cgtgcatctt catcacttct tgccccggtc ttaaccataa atggaaagtg
     2821 ttgcctaata aaatttgcgc ccctgtcgct ttaacttctt caggagtcat tccttttacc
     2881 gtgccataag tgccaacagg cataaatgcc ggtgtttcaa cgtgaaattc gcctttcggg
     2941 cggttaaaag ttaaacgccc acgacgagcg ttaccacttg ttgtttttaa ttcgtacttc
     3001 attttaatcc acaaataaac agttcgtgaa agcggtcaaa aaccaccaaa aatttaccaa
     3061 ttagaatcga aagtcggtgc cgacccctaa gccgattcca acgccgttag agccaccacc
     3121 ggctccaata ctgccaccca caccggttga tgagcaagca gctaacacta aaatgccaac
     3181 gcataataag agttttttca ttttttgctg tcctaaacga aaaagagagt ctatgataga
     3241 cccaatgtat tctaacctgt ttaatggatt aaacctaatt ttaaagggta aattatgaaa
     3301 attttacgta aattcgctgc tgtatgcgtt gtctcaatgg ctatgctctc tgctaacgct
     3361 aatttgctca ctattagcga gcaggaaatc aacgactatt tagccaccaa attagctgaa
     3421 aaaattcctt taaaagataa agtcggtatt cctaaattat ttgaattgga ttatcgcctg
     3481 cattcactta ccagccaaat tggcagaacc gaggaaaaaa aagtagctat ttctggcgtt
     3541 gttgatggac ttttaaacgc aaaaggcaag aaatatgctg cctctatcca tttaaatatg
     3601 gataccacgc cttattatga tccacaaaaa ggggcattat ttcttaaaga tgtcagattg
     3661 ttaagttggt ctgcaacacc ggctaaatat caagcagagc tgcaaatgtt tttgccggtg
     3721 ttggctgatg gattaagtag ttttttaaac agtacgcctg tttatacttt agatgaaaat
     3781 aaaacaaaag aagcaatggt gaaaaagttc ggcaaagcca ttgtagtaga aaatggtaat
     3841 atcaagctag aaaccactat tttttaaata attttgtgat cttcgtctaa tttttacact
     3901 ttgatagttg agcttttacg cgttttattt acaatccaaa tatcgccatt cattatttgg
     3961 agagttctat ggatatcggt attgttaaat ggtttaacaa tgtaaaagga tttggtttta
     4021 ttacatcgga tgtatgtgaa ggagacattt ttgctcattt ttctgaaatt caagcagaag
     4081 gttatcgctc tttaaaagtc ggccaaaaag tacagtttga attagttcaa ggagagcgag
     4141 gtgcctcagc ctcaaatatc acgcctataa gcgaaaaaac gttataatcc gtcgttaata
     4201 aaattattgt tataaccata ctttattaga ttttaaagct cagcttttac agctgagcca
     4261 tttttgcttt ttgaatatgc cttcctctta tcaattagtc ttaattaacc tactttatat
     4321 cttcggtatt ggtctaaata ttgtactgct taggtttctc agtttgcatt ttgacccatt
     4381 aaataataac ggtatacgtt ttcttgttgg tgggttcacc ttattatttt tcattttatg
     4441 gagattccgc acatcgctgt ctcagctgat taaaactcca aaattgattg cgatttctct
     4501 atttgtcggg ttaatgatgt ccgcaaatat gtatttttgg ctaaaagggg tcaaaattac
     4561 aaatgctgtt accgtctcta tttttggggt attagctatg ccatttggtg tgattattgc
     4621 cgccatattc tttcaagatg aaagagaacg cattaaaagc aaagctttct ggttcggctc
     4681 aattttaacg atcatcggtt cactcggatt tatttggcac gggaaaacac tctcgattgg
     4741 cgaaggattt attcttggtt ctgtgttctt atttttgtcg atcattatcc gtaatataca
     4801 aaacctagtt gtaaaatttg ctcgagaact gaacgtatta accctaagtt gtgtaacatc
     4861 tttaactact tcactctcga gtctattact tagccaacaa gcaggtaaat ttaatgagct
     4921 ttttactgca ccaaactggc tactattctc tctgatggtt gctggtattt atgcgattat
     4981 gatcggaatg gtattaacct ttcacattat ccaaacccaa ggactgataa cctaccaagt
     5041 attggaacta ttaatgccgg tttcaacagc catcattgct tatattttac tggacgaacg
     5101 tatttccatt gttcaaatgg gctttgcttg tattgtgatt ttaggctcaa gtatcgcctt
     5161 aaagttagta aaatatccta agcactatta ggatctttgc taattaatat tggtaaaatt
     5221 acatttggtt tactaatctg agcg
//
LOCUS       DANLZC010000153         5215 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.153, whole genome shotgun sequence.
ACCESSION   DANLZC010000153 DANLZC010000000
VERSION     DANLZC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.153"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..191
                     /locus_tag="PXE44_002238"
     CDS             <1..191
                     /locus_tag="PXE44_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL5589705.1"
                     /translation="FYGEEKGYRIARKHVGWYTEKLNPESNFKRTFNTLSSTKEQLYA
                     LEDFVKSIHLESQLRKVG"
     gene            196..492
                     /gene="fis"
                     /locus_tag="PXE44_002239"
     CDS             196..492
                     /gene="fis"
                     /locus_tag="PXE44_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDL5589706.1"
                     /translation="MLEQQPTQNPLTVTMLNAQAQQVNKPLRDNVKAALKNYLSQLNG
                     EDPTELYELVLSEIEHPMLDMVMQYTRGNQTRAATMLGINRGTLRKKLKKYGMG"
     gene            complement(597..3383)
                     /locus_tag="PXE44_002240"
     CDS             complement(597..3383)
                     /locus_tag="PXE44_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HDL5589707.1"
                     /translation="MKYHHFRYSPVALTVLFALSHSYGAATENKKIEENNDLAVLDEV
                     IVTESHYAHERQNEVTGLGKVVKNYHEMSKNQILGIRDLTRYDPGISVVEQGRGASSG
                     YAIRGVDKNRVSLLVDGLPQAQHYHTLGSDANGGAINEIEYENIRSIELSKGASSAEY
                     GSGALGGAIGFRTKDAQDIIKEGQHWGLDSKTSYASKNSHFLQSIAAAGEAGGFEALV
                     IATHRHGKETKIHSEANKLKHNIRRITGFENRYDLYPNSAQNAPGGSFFIVEDTCPTL
                     DCTPRARVKLNRDNFPVRTFPEYTPEERKQLEQIPYRTEQLSAQEYTGKDRIAPNPLD
                     YKSNSVFMKFGYHFNSSHYLGAILEDTKQRYDIRDMQTPAYYTKDDINLSLRNYVYEG
                     DNILDGLVFKPRIPYGLRYSHVKFFDERHHKRRLGFTYKYKPENNRWLDSIKLSADKQ
                     DIELYSRLHRLHCSDYPVVDKNCRPTLDKSWSMYRTERNNYQEKHRVIHLEFDKALNA
                     GQGVFKQTHKLNLGLGFDRFNSLMDHGDMTAQYTKGGYTSYRGRGRLDNPYIYRRDPR
                     SIETVSLCNNTRGDILNCEPRKIKGDSHFVSFRDLVISEYVDLGLGVRFDQHRFKSDD
                     PWTLSRTYRNWSWNGGITLKPTEFVSLSYRISNGFRVPAFYELYGKRNHIGLKDNEYV
                     QRAQRSHQLEPEKSTNHEIGVSFKGQFGYLDVSYFRNNYKNMIATACKRIIQKSHCFY
                     NYHNIQDVALNGINLVAKFDLHGILSMLPDGFYSSVAYNRVKVKERKLTDSRLDSVND
                     PILDAIQPARYVLGFGYDHPEEKWGIGITTTYSKAKNADEVAGTRHHGIHRVDLGGKL
                     TGSWYTHDITGYINYKNYTLRGGIYNVTNRKYSTWESVRQSGVNAVNQDRGSNYTRFA
                     APGRNFSLAFEMKF"
     gene            complement(3477..>5215)
                     /locus_tag="PXE44_002241"
     CDS             complement(3477..>5215)
                     /locus_tag="PXE44_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL5589708.1"
                     /translation="FVLLNAALLAACSSNGGSFDVQSAKVESQTQTTPKKPSLQDDNS
                     NARRTVSASETEALLQPGFGFSAKIPRRNLFPQGKEDVAPIGDIKEITGDLTKIPYEE
                     EVKAYGSSADGFSHTHDRNHKLYTRDFNFVRSGYVLHSGAKPEIKPKEILRTGAHGYV
                     YYLGIEPAKAIPTQKATYKGYWDFTTDARKGRDSKYFSNSAGINIGATPENSYDINVD
                     DSEKPMGHTGEFTADFANKTLTGTLVRNGYVSRSKEQKITTIYDIDAKIKGNRFSGKA
                     NAKNTDDPYFGKSSTTLEGGFFGGKAQELAGKFLADDKSVFVVFAGKRDAKKDDSESA
                     FDAFKIKLKDLNKSEMDTFGNATHLIINNKQIPLIAEGKKSFAEMKFDDLVTRTIDGK
                     TYRVSVCCNNLDYVKFGTYSEGNNSDTDTAHQYLVGERTAVADLPTGTVKYRGTWDGV
                     MYSKSGSAGAESPSNSESGTRSLFDVDFVNKKINGKLIANDGVEERPMLTLEGNLKGN
                     GFAGTAKTGNSGFNLDPKSTNGGTVVHINTQFEGGFYGPKATELGGIVHSAETDKDRV
                     SITFGGKRQIEK"
BASE COUNT         1556 a         1121 c          906 g         1632 t
ORIGIN      
        1 aattttatgg tgaagaaaaa ggttatcgta ttgcacgaaa acatgttggt tggtataccg
       61 aaaaattaaa tccggaatca aattttaaac gcacttttaa tacactaagt tccacaaaag
      121 agcaacttta tgcattagaa gattttgtta aatcgattca tttagaatct caattaagaa
      181 aagttggata aaaaaatgtt agagcaacaa cctacacaaa acccattaac agtaacaatg
      241 ttaaatgcac aagcgcagca ggttaataaa ccacttcgcg ataatgtgaa agcagcatta
      301 aaaaactatt tatctcaatt aaatggtgaa gatccaacag aattatacga attagtatta
      361 tctgaaatcg aacacccaat gttagatatg gtgatgcaat atactcgcgg caatcaaact
      421 cgtgcagcaa caatgctagg cattaaccgt ggcacattac gcaaaaaatt aaaaaaatac
      481 ggtatgggtt aatctaaccg accgttaaaa cacgatttta attcgtatgt aattagttag
      541 agccaacaaa aaacgctgaa tatactattc agcgtttttc tattttattt tgccgtctaa
      601 aacttcattt caaatgctaa actgaaattt ctccccggag cggcaaatcg agtgtaattg
      661 ctaccccggt cttggtttac tgcattcaca ccggattggc gcactgattc ccaagtggaa
      721 tatttacgat tagtcacatt ataaattcct ccacgtaagg tgtagttttt ataattgatg
      781 taaccggtaa tatcatgggt gtaccaagaa ccggtcagtt tgccacctaa atcaacgcga
      841 tgtatgccgt gatgacgtgt gcctgccacc tcatcggcgt ttttggcttt agaataggtg
      901 gtagtaatgc caattcccca tttttcttct gggtgatcgt agccgaatcc aagcacatag
      961 cgtgctggct gaatcgcatc tagaatagga tcgtttacgc tatcgagtct tgagtcggtt
     1021 agtttccgct cttttacttt tacacggtta taagcaactg atgaataaaa accatctggc
     1081 agcatagata aaataccgtg taagtcaaat ttagcgacta aatttatccc gtttagtgct
     1141 acatcttgaa tattatggta gttatagaaa cagtgtgatt tttgtattat tcttttacat
     1201 gctgtcgcaa tcatattttt atagttatta cggaaatagc tcacatcaag gtaaccaaat
     1261 tgacctttaa agctaactcc aatctcatga ttagtcgatt tttctggctc taactggtgg
     1321 ctacgttgcg cgcgttgcac atattcgtta tctttaagcc caatatgatt acgtttacca
     1381 taaagttcat agaatgcagg cactctaaaa ccgtttgaaa tgcgataaga aagcgataca
     1441 aactctgttg gtttaagcgt aatcccacca ttccaagacc aatttcgata agttcggcta
     1501 agtgtccacg gatcatcaga tttaaatcga tgttgatcaa aacgcacccc taatcccaaa
     1561 tccacatact cgcttatcac tagatcgcgg aagctaacaa aatggctatc gcctttaatt
     1621 ttacgcggtt cacagtttaa gatgtcgccg cgtgtattat tacacaaaga taccgtttca
     1681 atactgcgtg gatcgcggcg ataaatatat ggattatcta aacgccctct accgcggtag
     1741 ctggtataac cgcctttggt atattgggca gtcatatccc catgatccat aagcgaatta
     1801 aatcgatcaa agcccaaccc taaattcagt ttgtgggttt gcttaaatac gccttgacca
     1861 gcatttagcg ctttatcaaa ttctaaatga atgacacgat gcttttcttg gtaattatta
     1921 cgctcagttc gatacataga ccaagattta tccaaagtcg ggcggcaatt tttatctacc
     1981 acaggataat cgctacaatg caagcgatgt agccggctat atagttcaat atcttgttta
     2041 tccgcactga gtttaatgct atccaaccag cgattattct ctggtttata tttataggtg
     2101 aatcctaaac gacgtttgtg gtgacgttca tcaaaaaact tcacatggct atagcgcaac
     2161 ccataaggga tccttggctt gaacactaag ccatctaaaa tattatcccc ttcataaaca
     2221 tagttcctaa gtgataagtt aatatcgtct tttgtatagt aagctggcgt ttgcatatca
     2281 cggatatcgt agcgttgttt tgtatcttct aagattgcgc caagataatg agacgagtta
     2341 aagtgatagc caaacttcat aaaaacagaa ttactcttgt aatctaaagg gtttggtgca
     2401 atgcgatctt taccggtata ttcttgggct gagagctgct cagtgcgata aggaatctgc
     2461 tcaagctgtt tgcgctcttc aggcgtatat tccggaaatg ttctcactgg gaaattatcg
     2521 cggttcaact taacccttgc acgaggagta caatctaatg ttgggcaagt atcttccaca
     2581 ataaaaaaag atcctccagg agcattctgt gcggaatttg ggtaaagatc gtagcgattt
     2641 tcaaagccgg ttatacgccg aatattatgt tttaatttat ttgcctcgga atgaattttg
     2701 gtctctttac cgtgtcggtg agttgcaata acaagtgctt caaaaccacc cgcctcacca
     2761 gccgctgcga tagactgtaa aaaatggcta tttttgctgg cataagaggt cttactatct
     2821 aagccccaat gctgcccctc tttaataata tcctgcgcat ctttagtacg aaaaccaata
     2881 gcaccaccta gcgcaccaga gccatattcc gcagaacttg ctcctttgct taactcaatt
     2941 gaacgaatgt tttcatactc aatctcatta attgcaccac cattagcatc tgaacctagc
     3001 gtatgataat gctgcgcttg tggtagccca tcaacaagta agctgacacg gtttttatct
     3061 acacctcgaa tggcatagcc actacttgca ccgcgacctt gttccaccac cgaaatacca
     3121 gggtcatagc gagttaaatc acgaatacca agaatttgat ttttactcat ttcgtgataa
     3181 tttttcacta ctttccccaa gccagttact tcgttttgac gttcgtgagc ataatggctc
     3241 tctgtcacaa taacttcatc cagaacagct agatcgttat tttcttctat ttttttattt
     3301 tcagtcgcag caccgtatga atgagaaaga gcaaataaca ctgttaaggc aacaggtgaa
     3361 tagcgaaaat gatgatattt cattatcatt agatagggtc tcctttattt aaattttgca
     3421 aatttttata aaaaattgcc cgcttgctat ctacaaccag caaaggggaa ttatgattat
     3481 ttttctattt gacgttttcc gccgaatgta atactgactc tatctttatc cgtttctgca
     3541 ctatgtacaa taccacctaa ttccgtcgcc ttagggccat aaaagccccc ttcaaattga
     3601 gtatttatat gcactaccgt gccaccattc gtacttttcg gatcaagatt aaaaccagaa
     3661 ttgcccgttt tggctgtgcc tgcaaaacca ttccctttca gattgccttc aagtgtcagc
     3721 attgggcgtt cttcaacacc atcattagca atcagcttgc cattaatttt tttattgaca
     3781 aaatctacat cgaatagtga acgagtacca ctttcgctgt tacttggcga ttcagcccct
     3841 gccgagccag atttactgta cattaccccg tcccaagtac ctcgatattt tactgtccct
     3901 gttggcaaat ctgccacagc tgtacgttct cctactaaat attggtgagc agtatcagta
     3961 tcactattat ttccctcgct ataagtccca aatttgacat aatctaaatt attacagcag
     4021 actgaaactc gatacgtttt accatcaata gtacgggtaa ccaaatcatc aaatttcatc
     4081 tcggcaaagc tttttttgcc ttccgcaata agtggaatct gcttattgtt aataatcaaa
     4141 tgtgtcgcat tcccgaaagt atccatctca gatttattta aatctttaag tttaattttg
     4201 aaagcatcaa aggcagattc actatcatct tttttagcat ctcgtttgcc agcaaaaaca
     4261 acaaataccg acttatcatc agctaagaat ttaccggcaa gttcttgagc cttcccacca
     4321 aaaaatccac cttcaagtgt cgtagagctt ttaccaaaat aaggatcatc ggtatttttt
     4381 gcgtttgctt taccagaaaa gcgattacct ttaatttttg catcaatatc gtaaattgtt
     4441 gtaatttttt gctctttgct acgactaaca tacccattac gaaccaatgt tccagttaaa
     4501 gttttattag caaaatcagc cgtaaattct cctgtatgcc ccattggttt ttcagaatca
     4561 tcaacattaa tatcgtaact attttccggt gtggcgccaa tattgatgcc tgcggaatta
     4621 gaaaaatatt tactatctct cccttttctc gcatcggtag taaaatccca atatccttta
     4681 taagtagctt tttgagtagg tattgctttt gccggctcta tacctaaata gtaaacatac
     4741 ccatgtgcac ctgttctcaa aatttcttta ggctttattt caggttttgc accagaatgc
     4801 agaacatagc cggaacgaac aaaattaaaa tctctagtat acaacttatg atttctatca
     4861 tgagtatggc taaatccatc agcactacta ccgtacgctt taacctcttc ttcatacgga
     4921 atttttgtca gatctccagt aatctctttt atatcaccaa taggggctac atcttccttc
     4981 ccctgcggaa agagattacg acgcggaatt ttggctgaaa aaccaaaccc cggctgcaat
     5041 aaagcttcag tttcagaagc gcttactgta cgtcttgcgt tactattatc atcttgtaaa
     5101 cttggctttt tgggggtagt ttgcgtttga gattcaactt tggcagattg aacatcaaag
     5161 cttccaccat ttgaggaaca agcagcaagt agcgccgcat taagcagtac aaaac
//
LOCUS       DANLZC010000154         5192 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.154, whole genome shotgun sequence.
ACCESSION   DANLZC010000154 DANLZC010000000
VERSION     DANLZC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.154"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            35..2839
                     /locus_tag="PXE44_002242"
     CDS             35..2839
                     /locus_tag="PXE44_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL5589709.1"
                     /translation="MYKIKHSFNKTLIAISISSFLSIAYATESIENPQPIIQLSESLS
                     SKYSGKGVKLGVMDEGFMVKHPRHSSHLHPLIHQLTTPEGEVRIYDASYPQFEVNPVE
                     KEDGIDLIPSLETHGAGVAGIIAAQADKTLGDGYSGGIAKGAELYVATKSYKRTLEKV
                     IQDAKKELENAKDEEDEKTPSLDQMAKNDLLASKEKEMAIERAEWASGLNKLLDNNVF
                     AINNSWNPFSISDDINVVDKFYQSIKQNKHNPLLQAIMRAKNSNTLLVFAAGNESKKQ
                     PGVMALLPRYFPELEKNLISAVAVDKEQKIASYSNHCGASKNWCVAAPGDLHVLIGVA
                     DEHKKPQYGLTKEQGTSFSAPAITASLAVLKERFDYLTATQIRDTLLTTATDLGEKGV
                     DNVYGWGLINLKKAVNGPTQFLNDETITVTRDDHWSNPLASQFKITKKGDKSLHLDGE
                     NHLDTVAVEEGRLALNGKTKVKTISNHANLAVNGTEVEQNYSSSGQSQLEVLGKSGLI
                     ANAQANIHLAGSLKIDDKLTEKTEAGDVSATVVQLKDKATYQGGFTQLVENENLAKRG
                     LIQDLYFKESEIIAKVNKPLTDEKADTNGQAGLALLNALRTTPIAYRRSWYNGWLQSA
                     LEQRKLDNLHYAVSNNIYADSLELLRSQNAKGLTQAQQHLFTAYHTPLQTTVWAEHLN
                     QKQSASSKHTDVKHHQSQLGVNHKLADKTVLSATLSQQKNRLEKPFAQATLKQTALNI
                     GLRYHLDNAWFSEATLQFARQKYQQSRRFASHQLGTAETRGSTLGGEMRIGYQFMPNQ
                     WIIEPSLGVQWIQTKMNGLNESGELATQTAAMRYRDVNIVPSVKLQRTFQLEQGSISP
                     YIGLNYLHRLNGKITKITSNIAGKTLHSEATTKRNRQLNGEVGVKLHYKNWFTAMNLD
                     YSRVKSANLFGWKVNVGFSF"
     gene            complement(2980..3417)
                     /locus_tag="PXE44_002243"
     CDS             complement(2980..3417)
                     /locus_tag="PXE44_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL5589710.1"
                     /translation="MDWFFNWIGWSILGFVLLALELVLPGVFIMWWGFASLILAFIVA
                     LFTLGQALQIALFAIIATIFSLIWWKYQHGKDKKEDQSSSLNSREHAMLGAQGFVVEI
                     LPNGIARGKFGDTTWRVIGNNLQVADKIEVQKVEGITLFVRKQ"
     gene            complement(3453..4373)
                     /locus_tag="PXE44_002244"
     CDS             complement(3453..4373)
                     /locus_tag="PXE44_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDL5589711.1"
                     /translation="MNFDLPIVSIAFVVLVLVALSSTIKIVPQGYHWTVERFGRYTKT
                     LSPGLNIVVPFIDRIGRKMNMMEQVLDIPSQEVISRDNASVAIDAVCFVQTVDARRAA
                     YEVNHLEQAIVNLTMTNMRTVLGSMDLDDMLSQRDLINGRLLSIVDEATNIWGVKVTR
                     IEIRDVRPPKELVAAMNAQMKAERNKRADILEAEGIRQAEILRAEGEKQSRILKAEGE
                     RQEAFLQAEARERAAEAEAKATQMVSEAIAKGDTTAINYFIAQKYTEALKEIGSSDNS
                     KVVLMPLEAGNLIGSVAGIAEILKSNKTSS"
     gene            complement(4389..5078)
                     /gene="ung"
                     /locus_tag="PXE44_002245"
     CDS             complement(4389..5078)
                     /gene="ung"
                     /locus_tag="PXE44_002245"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL5589712.1"
                     /translation="MKTWTEALGEEKQKAYFQQILQYVHNERLAGKVIYPPQNEVFSA
                     FALTEFSDVKVVILGQDPYHGPNQAHGLAFSVKPPVAPPPSLVNMYKELSEDIEGFQI
                     PPHGYLIDWAKQGVFMLNTSLTVEQGRAGSHSQIGWEIFTDKVIAQLNQHRDNLVFLL
                     WGSHAQRKGQFIDRNRHCVLASPHPSPLSAYRGFFGCKHFSKANNYLISQGISPINWQ
                     LSVDVSYSSTH"
BASE COUNT         1733 a         1044 c          955 g         1460 t
ORIGIN      
        1 acgtaaaata agcttatttt ttggaggtaa aattatgtat aaaataaagc attcatttaa
       61 caaaacactt attgcaatta gcatctctag ttttttatct attgcatacg ctacagaatc
      121 aatagagaat ccacaaccta tcattcaatt atctgagagc ctcagctcaa aatatagtgg
      181 caagggtgtt aaactaggcg taatggatga gggcttcatg gttaaacacc ctcgccatag
      241 ttcacatctt catcctctta ttcatcaatt aacaacaccc gaaggcgaag taagaatcta
      301 cgatgcaagt taccctcaat tcgaggtaaa ccccgtagaa aaagaagatg gaattgatct
      361 tattccttct cttgaaacac acggtgcagg tgtagctggt attattgccg ctcaagcaga
      421 taaaactctt ggtgatggct atagtggtgg tattgcaaag ggagctgagc tttatgttgc
      481 aacgaagtca tataaacgaa ctttagaaaa agttattcaa gatgctaaga aagaattaga
      541 aaatgcaaaa gacgaagagg atgaaaaaac gccaagtctg gatcaaatgg caaagaatga
      601 cttattagca agcaaagaaa aagaaatggc aattgaacgt gctgaatggg catccgggct
      661 gaataaatta ctagataata atgtttttgc aatcaataat agttggaatc ctttttctat
      721 tagcgatgac atcaatgttg tagataaatt ttatcaatct atcaagcaaa ataagcacaa
      781 tccgctactt caagctatta tgagagccaa aaatagcaac accttattag tctttgctgc
      841 tggtaatgag agcaagaaac agccaggcgt aatggctctt ctaccacgtt actttcccga
      901 gcttgagaaa aatttaattt ctgccgttgc ggtagataaa gaacaaaaaa ttgcttcata
      961 ctctaaccac tgcggagcaa gtaaaaactg gtgtgtagca gcaccgggcg atttacatgt
     1021 tttaattggc gttgctgatg aacataaaaa accacaatat ggcttaacaa aagagcaagg
     1081 tacgtcattt tcagcacctg ctataactgc ttcattagca gtattaaaag aacgctttga
     1141 ttatctgact gcgactcaaa ttcgcgatac tttattgact accgccactg atttaggtga
     1201 aaaaggggtc gataatgttt atggctgggg tctcattaac ctgaaaaaag ctgttaatgg
     1261 cccaactcaa ttcctaaatg atgaaacgat taccgtaact cgtgatgacc attggtcgaa
     1321 tccactcgcc agccaattta aaattacaaa aaaaggggac aaatcactgc atttagacgg
     1381 cgaaaatcat ttagatacgg tagcagttga agaaggtaga ttggccttaa acggtaaaac
     1441 taaggtgaaa acaatcagta accatgcaaa tttagctgtc aatggaaccg aagttgagca
     1501 aaattattct tcgtctggac aaagccaatt agaagtgttg ggcaaatctg ggctaattgc
     1561 caatgctcaa gccaatatac atttagccgg ctcgttaaaa attgatgata aattaacaga
     1621 aaaaaccgaa gcaggtgatg tttcagcgac cgtagtacag ttgaaagata aagcaactta
     1681 tcaaggcgga tttacacaat tagtcgaaaa cgagaattta gccaaacgag ggttaatcca
     1741 agatctctat ttcaaagaga gcgaaattat cgcaaaagtg aataagccac taactgatga
     1801 gaaagccgat acaaacgggc aagccggttt agcattatta aatgcgttaa gaactacacc
     1861 tattgcctat cgccgtagtt ggtataacgg ttggctgcaa tctgcactcg aacaacgtaa
     1921 attagataat ctgcactatg ctgtatctaa caatatttat gcagatagcc ttgaattgct
     1981 acgtagccaa aatgccaaag gcttaaccca agcacagcaa catttgttta cggcatacca
     2041 tacaccactg caaacgactg tttgggctga acatttgaat caaaaacaat cagcctcatc
     2101 gaagcatacc gatgttaaac accaccaatc acaattggga gtaaaccaca aactggcaga
     2161 taaaacggta cttagtgcaa cactttcaca gcaaaaaaac cgcttagaaa aaccatttgc
     2221 acaagctacc ttaaaacaaa cagcattaaa tattggctta cgttatcatt tagataacgc
     2281 ttggtttagt gaagctacat tgcaatttgc tcgccagaaa tatcaacaaa gccgtcgatt
     2341 tgctagccat caactaggta ctgcagaaac cagaggttca acgctcggtg gtgaaatgcg
     2401 gatcggttat caatttatgc caaaccaatg gataattgaa ccaagccttg gcgtacaatg
     2461 gattcaaacc aaaatgaatg gtttaaatga aagtggcgaa cttgcgactc aaacggctgc
     2521 aatgcgttat cgtgatgtga atattgttcc aagcgtgaaa ttacagcgaa ctttccaact
     2581 tgagcaaggc tctatttccc cttatatcgg gctaaattac ttgcatagat taaatggaaa
     2641 aataaccaaa attaccagta acattgcagg aaaaacactg catagtgaag caacgacgaa
     2701 acgtaatcgc caattaaacg gcgaagtcgg ggtgaaactg cattataaaa attggtttac
     2761 tgcgatgaat ttagattaca gtcgagtgaa atctgcaaac ctatttggtt ggaaagtaaa
     2821 tgttggcttt agtttctaac ttattatggt gggacacaag cggttaattt agggtgaaaa
     2881 tttgcaaatt tcactctatt ttcaaccgct tgagagaaaa aaccttgaac aactgttcaa
     2941 ggtttttgtt tagtgttcta tgactaaata agaaaaaact tattgcttac ggacaaataa
     3001 ggtaattccc tccactttct ggacttcaat cttatctgct acttgtaaat tattacctat
     3061 aacacgccaa gtcgtatcac caaattttcc tcgagcgatg ccattaggta agatttcaac
     3121 cacaaagcct tgagctccta gcatagcatg ctctcgagag ttaagggaac tgctctgatc
     3181 ctctttcttg tcttttccat gctgatattt ccaccaaata agactaaaaa tagtcgcaat
     3241 tatcgcaaat aacgcaattt gtaacgcttg ccctaatgta aaaagtgcaa cgatgaaagc
     3301 aagaataagc gaggcaaatc cccaccacat aataaagaca cccggtaata ctaattctaa
     3361 tgcaagtagt acaaagccta atatgctcca gccaatccaa ttaaaaaacc agtccatact
     3421 ccctccatat actcaattaa tccggtttta ggctatgagc ttgttttatt gctttttaaa
     3481 atttcagcaa taccagcaac agaaccaatc aaatttccag cttctaacgg cataagtaca
     3541 actttactgt tatctgaaga acctatttct ttcaaagctt cggtgtattt ttgtgcaata
     3601 aaatagttaa tcgctgtagt atcgccttta gcaattgctt cagaaaccat ttgagtggct
     3661 ttagcttccg cttctgcggc tcgttcacga gcttctgcct gtaagaatgc ttcttgtctt
     3721 tcaccttctg cttttaaaat acgagattgt ttttcccctt cagcccgcag aatttcagct
     3781 tgtcgaatcc cttctgcttc aagaatatca gcacgtttat tacgctcagc tttcatttga
     3841 gcgttcattg ccgcaaccag ctctttcggt ggacgaacat cccgaatctc aatacgggtg
     3901 acttttactc cccaaatatt agtcgcctca tcgacaatgg aaagtaatct accgttaatt
     3961 aaatcacgct gagagagcat atcatctaga tccatagagc cgagcacggt acgcatattt
     4021 gtcatggtta aattaacaat tgcttgctct aaatgattca cttcataggc agctcggcga
     4081 gcatcaactg tttgaacaaa acatacagca tcaatcgcaa cacttgcatt atcacgtgaa
     4141 ataacctctt gcgatggaat atctaaaact tgctccatca tattcatttt tcgcccaata
     4201 cgatcaataa aaggtacgac aatgttcaac cccgggctta gcgttttagt ataacgacca
     4261 aaacgttcta ctgtccaatg atagccctgt ggcacaattt taatagtcga acttaatgca
     4321 actaaaacaa gcacaacaaa tgcaatacta acaatcggta aatcaaaatt catcgtttgc
     4381 tccttaaact aatgtgttga agaataacta acgtctactg ataattgcca attaattggg
     4441 gatattcctt gagaaatcaa gtagttattg gctttagaaa aatgtttaca accaaaaaat
     4501 ccacgatatg cggaaagtgg tgatgggtgc ggagaagcta acacacaatg gcgattacga
     4561 tcgataaatt gcccctttct ctgagcgtgg cttccccaaa gtagaaagac taaattatca
     4621 cggtgttgat ttaattgtgc gatcacttta tcggtaaaaa tttcccaacc aatttgtgaa
     4681 tgcgaaccgg ctctaccttg ctcaaccgtt aagctggtgt ttaacataaa taccccttgt
     4741 tttgcccagt caattaaata gccatgcggt ggaatttgaa atccttcaat atcttcagat
     4801 aattctttat acatattgac cagagaaggt ggcggggcaa ccggcggttt aactgaaaag
     4861 gctaatccgt gggcttgatt tgggccgtga taaggatctt gccctaaaat tactactttc
     4921 acgtcactaa attcggttaa ggcaaaagca ctaaacacct cgttttgcgg aggatagatg
     4981 actttgccgg ctaatcgttc gttatgtaca tattgtaaaa tttgttgaaa atacgctttt
     5041 tgtttttctt cgcctaaagc ttctgtccaa gttttcataa aaccaccaag tgtatatttt
     5101 ttacccaacc aactcaatca tgtctaaaaa tggttaaatt tttatcgctt tttatctaaa
     5161 aaggggtaca ataacttcaa ataaatttga at
//
LOCUS       DANLZC010000155         5181 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.155, whole genome shotgun sequence.
ACCESSION   DANLZC010000155 DANLZC010000000
VERSION     DANLZC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.155"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            45..725
                     /locus_tag="PXE44_002246"
     CDS             45..725
                     /locus_tag="PXE44_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HDL5589713.1"
                     /translation="MALNNILGLFAQSPLKPLQKHSNKVTECSELLESFFEATFEHDW
                     EKAAEVRGKIVDLERRADALKREIRLKLPRGLFMPVERGDLLELVTQLDKLANYSRDI
                     SGRIIGRKLIIPTEMQPLFKKFLSRSIDASRQVRKVLDEMDQLLETGFRGRELDFVNK
                     MILELDQIEDDTDQYQITLRHTLLELEKNLNPIDVMFLYKCIERISVLADQAQRIGSR
                     IELMLAKS"
     gene            737..2005
                     /locus_tag="PXE44_002247"
     CDS             737..2005
                     /locus_tag="PXE44_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDL5589714.1"
                     /translation="MELLNQYGSILVIITAIVGFMMAFGIGANDVSNAMGTSVGSGTI
                     TIKQAILIAMVFEFAGAYLAGGEVADTIKSGIIDPAQFANSPDILVLGMMASLCAAGM
                     WLIIATKMGWPVSATHTIIGAVIGFALITIGSDAIQWDALGGIVGSWFVTPILAGIVA
                     YSIFINSQKLIFNRSNPFKQAKKYGPMYMGITIFILVIVTVSKGLKHVGLHLDTAETL
                     GISFALALLAFIIGYFYFRSKSFAMKARAEEKGFAGVEKIFSILMLITACAMAFAHGS
                     NDVANAIGPLAAVESIVRSGGMIEGKTTLAPWILPLGALGMMIGLAIMGKNVMATVGT
                     GITDLTPSRGFAAQFACAVTVVIASGTGLPISTTQTLVGAVLGVGFARGIAALNLGVI
                     RNIVISWVITLPVGAVLSIIIYYILSAIFY"
     gene            2060..2671
                     /locus_tag="PXE44_002248"
     CDS             2060..2671
                     /locus_tag="PXE44_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDL5589715.1"
                     /translation="MKKLSSALLSCFLLGLSSPALTADTYITENLSTFMRKGAGDQFR
                     ISGAIQAGEKVTVLDRKERYSLIRDSRNREGWVLNSDLSDTASPKELIPQLKQQVQDL
                     TNRLSKIDTEWQQRTAEMQRRTQDSVQKSSDLVEENAQLKRELEILKNKNRDLETMHD
                     SEKREIVIQWFIYGGAVLGAGLLLGLLVPFLIPRRRRNNSGWA"
     gene            complement(2725..4023)
                     /gene="aroA"
                     /locus_tag="PXE44_002249"
     CDS             complement(2725..4023)
                     /gene="aroA"
                     /locus_tag="PXE44_002249"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL5589716.1"
                     /translation="MEKLTLTPISRVEGEINLPGSKSLSNRALLLAALATGTTQVTNL
                     LDSDDIRHMLNALKALGVKYELSDDKTVCVVEGIGGAFKVQNGLSLFLGNAGTAMRPL
                     AAALCLKGEEKSQIILTGEPRMKERPIKHLVDALRQVGAEVQYLENEGYPPLAISNSG
                     LQGGKVQIDGSISSQFLTALLMSAPLAEGDMEIEIIGDLVSKPYIDITLSMMNDFGIT
                     VENRDYKTFLVKGKQGYVAPQGNYLVEGDASSASYFLASGAIKGKVKVTGIGKKSIQG
                     DRLFADVLEKMGAKITWGEDFIQAEQSPLKGVDMDMNHIPDAAMTIATTALFAEGETV
                     IRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDFIRIQPLALENFQHAEIETYNDHR
                     MAMCFSLIALSNTEVTILDPNCTAKTFPTYFRELEKLSVR"
     gene            complement(4069..5163)
                     /locus_tag="PXE44_002250"
     CDS             complement(4069..5163)
                     /locus_tag="PXE44_002250"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL5589717.1"
                     /translation="MQFINIANEGVKSLSPYQAGKPIEELERELGIQNIIKLASNENP
                     FGFPESAKKAILNQLDDLTRYPDSNGFELKTTIAQKFGVQANQITLGNGSNDLLELFA
                     HTFAGEQDEIIYSQYAFIVYPLVTKAINAVAREILAKNWGHNLDAFLAAITEKTKLIF
                     IANPNNPTGNFLTQAEIEGFLAKVPANVIVVLDEAYTEFTAPQERVDSFGLLAKYPNL
                     IVSRSLSKAYGLAGLRIGYAVSNPEIADLLNRVRQPFNCNSLALASAIAVMNDDEFVK
                     KVAENNRLEMARYEAFCQANGLEYIPSKGNFITIDFKRPAAPIYEALLREGVIVRSIA
                     GYGMPNHLRISIGLPQENERFFTALLKVLA"
BASE COUNT         1475 a         1057 c         1054 g         1595 t
ORIGIN      
        1 acagtaagaa gtcattgtca atattatcac tatggagata ttttatggca ttgaataaca
       61 ttttaggttt attcgcccag tccccactta aaccgttaca aaaacactca aataaagtga
      121 ctgaatgtag cgagttatta gaatcttttt ttgaagcgac ttttgaacat gattgggaaa
      181 aagcagctga agtgcgcggt aaaattgttg atttagaacg ccgcgcagat gcccttaaac
      241 gtgaaatccg cttaaaactg cctcgtggtt tatttatgcc agttgaacgc ggtgatttat
      301 tagaactagt cacacaatta gataagttag caaactattc aagagatatt tccggtcgca
      361 ttattggtcg taaattaatc attcctaccg aaatgcaacc acttttcaaa aaattccttt
      421 ctcgtagtat tgatgctagc cgtcaggtac gcaaagtttt ggatgaaatg gatcagttat
      481 tagaaactgg ttttagaggg cgagagcttg atttcgtcaa taaaatgatt cttgaacttg
      541 accaaattga agatgatacc gaccaatatc aaattacatt acgtcataca cttcttgagt
      601 tagaaaaaaa cctgaaccct attgatgtga tgtttttata caaatgtatt gaaagaatca
      661 gcgttctagc tgaccaagcc cagcgaattg gttctcgtat tgaacttatg ctggcgaagt
      721 cataggagaa aattaaatgg aattactcaa tcaatatggc tcaatactgg tgattattac
      781 tgctattgta gggtttatga tggctttcgg tattggtgct aatgatgttt caaatgccat
      841 gggaacatcc gtcggctctg gaacaattac gattaaacaa gctattttaa ttgctatggt
      901 atttgaattt gctggtgctt atctggcagg cggtgaagtc gcagatacaa ttaaaagtgg
      961 cattattgat ccggcacaat tcgccaatag ccctgatatc ctggtacttg gaatgatggc
     1021 atctctctgt gctgccggaa tgtggctaat tattgcaact aaaatgggtt ggccggtttc
     1081 agcaactcac acaatcattg gtgccgttat tggttttgca ttaatcacta ttggttcaga
     1141 tgcaattcaa tgggatgcat taggtggaat tgttgggagt tggtttgtga cccctattct
     1201 tgcggggatc gtagcatatt cgatctttat taactcacaa aaactgatct ttaaccgctc
     1261 aaacccattc aaacaagcga aaaagtatgg cccgatgtac atgggaatta ctatttttat
     1321 tttagttatt gtaaccgtgt caaaaggctt aaagcacgta ggattacatc tagatacagc
     1381 tgaaacattg ggaatttcat tcgcgcttgc cttacttgct tttatcattg gatattttta
     1441 tttccgcagt aaaagctttg caatgaaagc tcgtgcagaa gagaaaggct tcgctggcgt
     1501 tgagaaaatt tttagtattt taatgcttat tacggcctgt gctatggctt ttgctcatgg
     1561 ctctaatgat gttgctaatg ctattgggcc tctagctgct gttgaatcta ttgtaagaag
     1621 tggtggaatg attgaaggta aaacgacttt agccccttgg attctcccac taggcgcctt
     1681 aggtatgatg attggtttag ctattatggg caagaatgta atggctacag taggtactgg
     1741 tattaccgac cttactccaa gccgtggctt tgctgctcaa tttgcctgcg ccgtcacggt
     1801 tgtcattgca tcaggtactg gtttaccaat atcaacaacc caaacgcttg taggagcagt
     1861 attaggtgtg ggattcgccc gtggtattgc tgcacttaac ttaggcgtta ttcgtaatat
     1921 tgttatatca tgggtaatta ccttgcctgt aggtgctgtg ctttcaatta ttatctatta
     1981 tattttaagc gctattttct attaattttt caaaaagaat acaggcaact gtattcttcc
     2041 tagctttaga ggaatatata tgaagaaatt atcatcagct cttctgtctt gttttttact
     2101 cggcctatct agccctgctt taacggcaga cacttacatt actgaaaatc tgagtacatt
     2161 tatgcgcaaa ggtgctggtg atcaattccg catttcaggc gctattcaag caggtgaaaa
     2221 agtcacagta ttagatcgta aagagcgcta cagtctgatt cgagatagtc gtaaccgtga
     2281 aggttgggta cttaattcag acttaagcga tactgctagt ccaaaagagc tgattcctca
     2341 attaaaacaa caggttcaag atttaaccaa tcgcctgagc aaaatcgata ccgaatggca
     2401 acaacgtacc gcagaaatgc aacgtagaac gcaagattca gtgcagaaaa gcagtgattt
     2461 ggttgaggaa aatgcacaac taaaacgtga actggaaatc ctgaaaaaca aaaatcgtga
     2521 tttagaaaca atgcacgatt ccgagaaacg tgaaatcgtg attcagtggt tcatctatgg
     2581 tggtgctgtg ttaggcgcag gtttactact tgggttactc gtgcctttcc tgatccctcg
     2641 ccgcagacgt aacaacagcg gttgggctta gtctgccaca ataaaacgta aaaaggattc
     2701 agttttctga atcctttttt acttttatct gaccgataat ttttccaact ccctaaagta
     2761 agtcgggaac gttttagcgg tacaatttgg atctaagatc gtcacttctg tattcgataa
     2821 cgcaattaat gaaaaacaca ttgccatacg gtgatcgtta taggtttcaa tttcagcgtg
     2881 ctggaagttt tctaacgcaa gcggttgaat ccgaataaaa tcttcccctt cttctacctc
     2941 tgccccgact ttacgcaatt cggttgccat tgctgtcaag cggtcggttt cttttacccg
     3001 ccagttataa atattgcgga taactgtttc tccttcggca aataaagcgg ttgttgcaat
     3061 cgtcattgcc gcatcaggaa tatgattcat atccatatct acgcctttta gcggggattg
     3121 ctcggcttga ataaaatcct ctccccaagt gatttttgcc cccatttttt ccaacacatc
     3181 ggcaaacaag cggtcgcctt ggatcgattt tttaccaatg cccgttactt ttaccttgcc
     3241 tttaatcgca ccggaggcta agaaataaga ggcagaagag gcatctccct ccaccaaata
     3301 attaccttgt ggagcaacat agccttgttt acctttaact aaaaaggttt tgtaatctcg
     3361 attttcaacc gtaataccaa aatcgttcat catcgaaagg gtaatatcaa tataaggttt
     3421 tgataccaga tcaccgataa tctcaatttc catatcgcct tccgctaatg gggcagacat
     3481 cagcaatgcg gttagaaatt ggctggaaat cgagccgtca atttgcactt ttccgccctg
     3541 caaaccgcta ttgctaattg ccaacggtgg atagccttca ttttctaaat actgtacctc
     3601 tgcccctact tggcgtaaag catcgactaa gtgtttaatc gggcgttctt tcattcttgg
     3661 ttcaccggta agaatgattt gggatttttc ctcacctttt aaacacaatg ctgctgcaag
     3721 tggtcgcatt gccgtgcctg cattgccgag aaacagtgat aagccgtttt gaaccttaaa
     3781 agctccacca atcccttcaa ctacacagac ggttttatcg tccgatagct catatttcac
     3841 gcctaacgct tttaaggcat tgagcatatg tcgaatatca tcactatcta ataaattggt
     3901 cacttgagtc gtaccggtgg ctaaggcggc taataataag gctcggttag acaggctttt
     3961 agaaccaggt aaattgatct cgccttctac tcgggaaatc ggggttaaag ttagtttttc
     4021 catttttctc tttaaatttg taaaattttc ataaaaggta accgcttgtt aagctaacac
     4081 tttcaataag gcagtaaaaa atctctcgtt ttcttgcggt aaaccgatac taatgcgtaa
     4141 atgattcggc attccataac ctgctatcga gcgaacaatc acgccttcac gcagtaatgc
     4201 ctcataaatc ggagcggcag gacgtttgaa atcaatagta ataaaattgc ccttagaagg
     4261 gatatattcc aagccgtttg cttggcagaa agcctcatag cgagccattt ccaagcggtt
     4321 attttctgcc acttttttga caaattcatc atcattcatt accgcaatcg ctgaagctaa
     4381 ggcaagacta ttgcagttaa acggctggcg aacacgattt aataaatccg caatttcagg
     4441 gtttgaaacc gcataaccaa tccgaagccc ggctaagccg taagccttag agagtgaacg
     4501 tgacacaatt aaattcgggt attttgccaa taagccaaac gaatccaccc gttcttgagg
     4561 cgcagtaaac tcggtataag cttcgtccag caccacgatt acatttgccg gcactttagc
     4621 taaaaagccc tcaatttccg cttgggttaa aaaattaccg gtcggattat tcgggttggc
     4681 aataaaaatt aatttagttt tttcggtaat tgcagccaaa aaggcatcta aattgtgtcc
     4741 ccaattttta gccagaattt cacgggcaac ggcattaatc gctttcgtca ccaacggata
     4801 cacaataaac gcatactgag aatagataat ttcatcttgc tcaccggcga aggtgtgggc
     4861 aaacagctct aataaatcgt ttgagccatt gcctaaggtg atttgattgg cttgtacgcc
     4921 aaatttttgg gcaatagtgg tttttaattc aaagccgttt gaatccggat agcgggttaa
     4981 atcgtcaagc tgattcagaa tcgccttttt tgcactttca ggaaaaccaa atgggttttc
     5041 gttcgaggct aatttaatga tattttgaat ccctaattca cgctctagct cctcaatcgg
     5101 ctttcctgct tggtaaggcg aaagggattt cacgccctca tttgcgatgt tgataaattg
     5161 cataataacc tctgattatt c
//
LOCUS       DANLZC010000156         5017 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.156, whole genome shotgun sequence.
ACCESSION   DANLZC010000156 DANLZC010000000
VERSION     DANLZC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.156"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            182..667
                     /locus_tag="PXE44_002251"
     CDS             182..667
                     /locus_tag="PXE44_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HDL5589718.1"
                     /translation="MKIKTLSLIMLLSVTVNAAPVELHQFDSPKQESDYRALIQELRC
                     PQCQNNNIADSNATIATDMRAKTLELLKQGKSKDEVVDYMIERYGNFVTYDPPLTPAT
                     IMLWLLPLILIALGLGVVLRRKKNKPQAVNFEQNTAKPTTHLAQDEQERLNQILNKEE
                     K"
     gene            664..1575
                     /gene="ccmI"
                     /locus_tag="PXE44_002252"
     CDS             664..1575
                     /gene="ccmI"
                     /locus_tag="PXE44_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HDL5589719.1"
                     /translation="MNFWLAIIFVTLIVCGVAFYPLLKKNKQIDNTKRDSLNKAFYFD
                     RLKEVENEANEGVIDDPEQTKLELQKNLLDDIPHSTQSATSEKLTLGKSWFVALILFV
                     GGISFAIYSNVGSWFSNSMLDTTHQKLDSFYERLKTEETNPLSQEEMNQFAMALRVDL
                     QSNPNDDKKWYMLGQIGMALDDGQLAHDAFSKAAQLKPDNLDYKLSHAQLLLFSEAQE
                     DKKQGETLLKEIIRQDHTNFNALSLLAFHSFEKEDFKMAAMTWGMMLKLLPDEDPSKV
                     TVERSFNMALSNLSEAEKQELQKGEKK"
     gene            1572..2177
                     /gene="mog"
                     /locus_tag="PXE44_002253"
     CDS             1572..2177
                     /gene="mog"
                     /locus_tag="PXE44_002253"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDL5589720.1"
                     /translation="MTACKPLSKEKLKIGLVSVSDRASQGVYSDQGIPELRAWLESAL
                     TEPFELETRLIPDEQAIIEQTLIELVDAHACHLVLTTGGTGPAKRDVTPDATLAVAHR
                     EMPGFGEQMRQVSLHFVPTAILSRQVGVIRHDSLILNLPGQPKAIKETLEGVKDEQGK
                     VLVKGIFSAVPYCLQLLSEIYVETHAAICESFRPKSARREN"
     gene            2187..2525
                     /gene="glnB"
                     /locus_tag="PXE44_002254"
     CDS             2187..2525
                     /gene="glnB"
                     /locus_tag="PXE44_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDL5589721.1"
                     /translation="MKKIEAIIKPFKLDDVREALTDVGVSGMSITEIKGFGRQKGHTE
                     LYRGAEYAIDFLPKVKVEIVIPDELEEQCIEAIMETAQTGKIGDGKIFVYDVGRVIRI
                     RTGEENEEAI"
     gene            2606..3085
                     /gene="lspA"
                     /locus_tag="PXE44_002255"
     CDS             2606..3085
                     /gene="lspA"
                     /locus_tag="PXE44_002255"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL5589722.1"
                     /translation="MKTGLKFLWVSLIVIIIDLWSKYIVVQHFEFGESINILPIFNFT
                     YARNYGAAFSFLADHSGWQKYFFLGLAVVISSALVVALWKNQAVKKLENIAYALIIGG
                     AIGNAIDRAYNGYVVDFLHFYWDIYHYPVFNIADIAICVGAGLLILEAFITKKEPKK"
     gene            3096..4040
                     /gene="ispH"
                     /locus_tag="PXE44_002256"
                     /gene_synonym="lytB"
     CDS             3096..4040
                     /gene="ispH"
                     /locus_tag="PXE44_002256"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDL5589723.1"
                     /translation="MKILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNKF
                     VVDGLKAKGAIFVEELHEVPDDAIVIFSAHGVSQVVRQEAKNRGLKVFDATCPLVTKV
                     HMQVARASKKGTKAILIGHEGHPEVIGTMGQYNNQEGGIYLVESVEDIANLPVSNNDE
                     LTFMTQTTLSIDDTIGVIEALKEKYPAIQGPRKNDICYATTNRQHAVRELAEQSQLVL
                     VVGSKNSSNSNRLAELASRMGVMSKLIDGPQDIDPTWLEGIETIGITAGASAPEVLVQ
                     SVISHLKTLGVTEVETLVGNEEDVVFEVPKELRIKEVK"
     gene            4040..4288
                     /locus_tag="PXE44_002257"
     CDS             4040..4288
                     /locus_tag="PXE44_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HDL5589724.1"
                     /translation="MTEINRFKLEWACRRGMRELDKMIMPFYQNHFNELNETQQQAFV
                     EMLNYSDPELFRWVMLQAPAPTEEIAELIRLMRDKIET"
     gene            complement(4495..>5017)
                     /locus_tag="PXE44_002258"
     CDS             complement(4495..>5017)
                     /locus_tag="PXE44_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5589725.1"
                     /translation="NQSANQYIFNSDWFTHNVPSLASIFEYVKPRRILEIGSFEGRSS
                     VFFIEEALKYQSQVEIHCIDSWEGGAEHQGVWDMGEIEQRFIHNVTLAQQKFQNVVLY
                     KHRGYSHQKMIELLANGYINYFDYIYIDGSHEAPDVLFDALLAHRLVRKGGVISFDDY
                     LWSPDETGKQRHY"
BASE COUNT         1613 a          999 c         1066 g         1339 t
ORIGIN      
        1 tataaaaata acaataaact tcacatggac aaaactaaga taacactaga aaaaggagat
       61 aaggttactt attatatgat ctgtggaaaa aagctatttg aaaacacaga aacttttgta
      121 ctggaaattg aaaataacaa atatcaaatt cctaataaat atatcaacaa tattcaaaat
      181 tatgaaaata aaaactctca gcctaataat gcttctaagt gtaacagtta atgctgctcc
      241 ggtagaactg caccaattcg acagcccgaa acaagagtcg gactatcgtg ccttaattca
      301 agaactgcgt tgcccgcagt gtcaaaataa taatatcgcc gattccaatg cgactattgc
      361 caccgatatg cgggcaaaaa ccttagaatt gctcaaacaa ggcaaaagca aagacgaagt
      421 ggtggattat atgattgaac gctacggcaa tttcgtcact tacgacccgc ctctcacacc
      481 ggcaaccatt atgttatggc tgttgccgtt aattttaatt gccttagggc tgggggtggt
      541 gttgcgtaga aagaaaaaca aaccacaagc ggtcaatttt gagcaaaata ctgcaaaacc
      601 gaccactcat ctcgctcaag atgagcaaga acgtcttaac caaattctca ataaggaaga
      661 aaaatgaatt tctggctggc aattattttc gttacactga ttgtatgtgg cgtggcgttt
      721 tatccgttac ttaaaaagaa caagcaaatc gataacacca agcgagacag cttaaacaaa
      781 gccttctatt ttgaccgctt aaaagaagtg gaaaacgaag cgaatgaagg ggtgattgat
      841 gaccctgaac aaaccaaatt agagctacaa aaaaacttgc ttgatgatat tccacacagc
      901 acacaaagtg ccacaagtga aaaattaacg ctcggcaagt cgtggtttgt ggcattgatt
      961 ttatttgtcg gcggtatcag ctttgcgatt tacagcaatg tcggttcttg gttctccaac
     1021 tcaatgttag acaccactca ccaaaaatta gacagcttct atgagcgttt aaaaaccgaa
     1081 gagaccaatc cgctctcaca agaggaaatg aaccaatttg cgatggcatt acgggtagat
     1141 ttacaaagta atccgaatga cgacaaaaaa tggtatatgc tcggtcaaat cgggatggcg
     1201 ttagatgatg gtcagcttgc acacgatgcc tttagcaaag cggctcaact caagccggat
     1261 aacttagatt acaaactcag ccacgcccaa ctgctgctct tttccgaagc acaggaagac
     1321 aaaaaacagg gtgaaacctt gttaaaagag attatccgcc aagaccacac aaactttaat
     1381 gcgttaagtt tattggcgtt ccactccttt gaaaaagaag atttcaaaat ggcggcaatg
     1441 acgtggggaa tgatgttaaa actactgcct gatgaagatc ctagcaaagt aacggtagaa
     1501 cgcagtttta atatggcgtt atccaattta agtgaggcgg aaaaacaaga attgcaaaaa
     1561 ggtgagaaaa aatgaccgct tgtaaaccac ttagcaaaga gaagttgaaa atcggcttgg
     1621 tgtcggtttc agaccgagcc tcgcaggggg tttattcaga ccaaggcatt cccgaattaa
     1681 gggcgtggct tgaatctgcc ttaaccgagc cgtttgagct ggaaacccgc ttaattcccg
     1741 atgagcaagc cattattgag caaaccttaa tcgagttagt cgatgctcac gcttgccatt
     1801 tggtattaac caccggcggc accggcccgg caaaacgtga tgtcacccca gatgccaccc
     1861 ttgctgtcgc ccaccgagaa atgcccggct ttggcgaaca gatgcgacaa gtgagccttc
     1921 acttcgtgcc aaccgccatt ctctctcgcc aagtgggagt aattcgccac gacagtctga
     1981 ttttaaatct gccggggcaa ccgaaagcaa ttaaagaaac gctggaaggt gtgaaagatg
     2041 aacagggcaa agtattggtt aaaggtattt ttagtgccgt gccatactgt ttacaacttt
     2101 taagcgaaat ttatgtcgaa acccacgcag ctatttgcga aagtttcaga cccaaatcgg
     2161 caagacgaga aaattaaggc taaaaaatga aaaaaataga agcaattatt aaacctttta
     2221 aattagacga tgtgcgtgaa gccttaaccg atgtgggtgt cagcggaatg agtattaccg
     2281 aaatcaaagg gtttggcaga caaaaaggac ataccgagct ttatcgtggg gcggaatatg
     2341 cgattgattt tctaccgaaa gtgaaagtcg aaatcgtgat tcccgacgag ttggaagaac
     2401 agtgcattga agcaattatg gaaaccgccc aaaccggcaa aatcggcgat ggcaagattt
     2461 ttgtgtatga tgtcgggcgg gtgatccgca ttcgtaccgg cgaagaaaat gaagaagcga
     2521 tttagttttg caaaaaatcc ggcaaaaacg accgcttgca gggggatttc catctactct
     2581 ttttacatta aattttggat attaaatgaa aacaggctta aaatttcttt gggtgagttt
     2641 aattgtaatc atcattgacc tttggtcaaa atacattgta gtgcaacatt ttgaatttgg
     2701 tgaaagcatt aatattttgc cgatttttaa cttcacttac gcccgaaact acggggctgc
     2761 ctttagtttc cttgccgacc attccggctg gcaaaaatat ttctttttag ggctggcggt
     2821 agtgatttcc agtgctttag tggtggctct ttggaagaat caagcggtca aaaaattaga
     2881 aaatattgcg tatgccttaa ttattggtgg agcgattggg aatgccattg accgtgctta
     2941 caacggctat gtagttgatt tccttcattt ttattgggat atttatcact accctgtgtt
     3001 taatattgcc gatattgcga tttgtgtggg tgcaggctta ctgattttag aagcctttat
     3061 aaccaaaaaa gaaccgaaaa aataagagtg attaaatgaa aattttatta gcaaacccac
     3121 gcggtttttg tgccggcgtg gatcgagcca tttcgattgt tgaattagcc ttagaaattc
     3181 acggtgcacc tatctatgtt cgccacgaag tggtacataa caaatttgtg gtggatggct
     3241 tgaaagccaa aggggctatt tttgttgagg agctacacga agtgccggac gatgctatcg
     3301 tgatcttctc agcccacggg gtgtcgcaag tagttcgcca agaagccaaa aatcgtggct
     3361 taaaagtgtt tgatgccact tgcccactcg tgaccaaagt gcatatgcag gttgcccgag
     3421 ccagtaaaaa agggacaaaa gcaattttaa tcggtcacga aggtcaccct gaagttatcg
     3481 gcacaatggg acaatacaat aaccaagaag gtggcattta tttggtagaa tcggtggaag
     3541 atattgccaa cttgccggtt tccaacaacg atgaattaac ttttatgacg caaaccacac
     3601 tttccattga cgataccatt ggcgtgattg aggcgttaaa agagaaatat ccggcaattc
     3661 aaggccctcg caaaaatgat atttgttatg ctaccaccaa tcgccaacac gcagtgcggg
     3721 aactggcaga acaatcacaa ttagtgttag tggtaggttc aaaaaactcc tcgaactcaa
     3781 atcgtttggc ggaactcgcc tctcgaatgg gtgtgatgtc aaaactgatt gatggtccgc
     3841 aagatattga tccaacttgg ttggagggta ttgaaactat cggtattacc gccggtgctt
     3901 ccgctcctga agtgttggtg caatcagtaa tttcgcattt aaaaaccctt ggcgtaaccg
     3961 aagttgaaac cttagtcggc aacgaagaag acgtggtgtt tgaagtcccg aaagagttac
     4021 gcatcaaaga ggtaaaataa tgacagaaat caaccgcttt aaattagaat gggcgtgccg
     4081 acgtggtatg cgtgagttag acaaaatgat tatgccgttt taccaaaatc attttaatga
     4141 actcaatgaa acccaacagc aagctttcgt tgaaatgctc aattacagcg acccggaact
     4201 gtttcgctgg gtaatgctgc aagcacctgc cccaaccgaa gaaatcgctg aattgattag
     4261 gttaatgcga gataaaattg agacctagag ctaatagatt aacctcttct ttggggtaaa
     4321 tatatagcta cttaccccaa agaagactaa attcattcaa cttttacaaa ataaccctaa
     4381 actagaaaag ttaatcttcc aatttaataa tatgttgctg ataagctatt aaaccttgta
     4441 ttacatgcac tttctgctga taggtattga catagtgatc aacagcgggt tttactaata
     4501 atgacgctgt ttacctgtct catctggtga ccaaaggtaa tcatcaaaac taataacgcc
     4561 tccttttctc actaaacgat gtgcaagtaa agcatcgaac aacacatctg gcgcttcgtg
     4621 agatccatct atgtaaatat agtcaaaata atttatatat ccatttgcga gaagttcaat
     4681 cattttttgg tgagaatagc cacggtgctt atataacaca acattttgga atttctgttg
     4741 cgcaagagtg acattatgga taaatcgttg ttctatttct cccatgtccc aaactccttg
     4801 atgctctgct cccccttccc aactatcaat acaatgaatt tcaacttgtg attgatattt
     4861 caatgcttct tcaatgaaaa agacagatga tctaccttca aatgatccaa tttctaaaat
     4921 cctccgaggt tttacatatt caaaaataga agctaatgaa ggaacattat gagtaaacca
     4981 atcagagtta aaaatatatt ggttggctga ctggttg
//
LOCUS       DANLZC010000157         4930 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.157, whole genome shotgun sequence.
ACCESSION   DANLZC010000157 DANLZC010000000
VERSION     DANLZC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.157"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..398
                     /locus_tag="PXE44_002259"
     CDS             <1..398
                     /locus_tag="PXE44_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL5589726.1"
                     /translation="KLQKYKCRIINVVDKTALVSKNSTLGIGVFVGKMAIVNSGVTVG
                     DNVIINTKSLVEHGCFIGSHCNISTNTTLNGDVIVEDHAFIGSSSVVNGQLRVGESAL
                     VGSGAVVIRNVEPRTVVAGVPAKYIKDIK"
     gene            398..1435
                     /gene="neuB"
                     /locus_tag="PXE44_002260"
     CDS             398..1435
                     /gene="neuB"
                     /locus_tag="PXE44_002260"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL5589727.1"
                     /translation="MVNVFIVAEIGCNHNGDPALAKKMVDVAKECGVDAVKFQTFKAD
                     KLISKYAPKAEYQKVTTGTADSQLEMTRKLELPYEEFVKLEAYARSLGLEVFSTPFDF
                     ESIDFLASQQQKVWKIPSGELTNLPYLEKIARLPIEGKTIVISTGMATIEETKASLKV
                     LEDNGVAKKDITILHCNTEYPTPYEDVNLNAFHQLKQEFGDYSLGFSDHSAGYFAGLA
                     AVPYGITFIEKHFTLDKNFEGPDHKASVTPEELKLLCEGIRAVEKSLGSSEKLVTNSE
                     AKNKIVARKSIIAARPIKKGEIFTTENITTKRPGNGISPMFWYDVLGKEAQQDFEEDQ
                     LIQDSRFENQM"
     gene            1495..2754
                     /locus_tag="PXE44_002261"
     CDS             1495..2754
                     /locus_tag="PXE44_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL5589728.1"
                     /translation="MKKIAIITARAGSKGLPNKNVLLANGKPLMAYSIEAAIESGQFE
                     KIIVSTDSQEYIDLFSHYPIEFIKRAEHLASDKASSFVVIEDVLNQYRSVEFDYFMLL
                     QPTSPLRSGEHIKESCEKFEKNFEQFDFLVSVSDAHKPTTLTRMIEEDECLKHFQLDY
                     SNYARQLHHPEYSPNGAIFTAKPEAYLKQKHFYGAKCLAYFMDKDVSIDIDDRQDFEY
                     FYFILQQRNKEKILLETIKRSILMKKENFKEVKEIALIGHSIIEYWDIEHLNGKAVNN
                     LGIAGISTKQYSDLILDEGLISALPKDIILMFGTNDIVYAGWSDEKILADINHLIAQL
                     TAIRGDINLYFLEITPTAFRVDRNNNDIRLLNQYLKDNLAVKWISLDQAFSDKYGKLD
                     LSYSDDGLHLNAKGYEKLTAILEKELN"
     gene            2755..3939
                     /gene="neuC"
                     /locus_tag="PXE44_002262"
     CDS             2755..3939
                     /gene="neuC"
                     /locus_tag="PXE44_002262"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL5589729.1"
                     /translation="MKNIAYITGSRAEYGIVKRLLQKLAQDPTVNFSLVVTAMHLDPQ
                     YGNTVTVIEQDGFEIAARIPLTLNSENNQTIIHSMAECLDRFGEHFQQHRYDAVIVLG
                     DRYEMLAVATAAAMHNIPLIHLHGGEQTLGNYDEFIRHCITKMSKLHLTSTEEYRQRV
                     IQMGELPENVVNTGSLGAENSLDLVLPTQAELVERLGIPASPYFLVVFHPETLTDVSV
                     LEQIEALLNAFEQFKDKYQFVFIGSNSDTNSEQIYARFKAYTDENQFKFFTSVKPEEY
                     LALIKYSNGLIGNSSSGLIEAPSCGVGTLNIGNRQQGRVRDESVIDVESNEQAIIDGI
                     EKLISADFQHRLPSMKNPYFQDNAMEKAYNAIKIFLADSHHTQPKGFYDVAFSQAVKK
                     SE"
     gene            3949..>4930
                     /locus_tag="PXE44_002263"
     CDS             3949..>4930
                     /locus_tag="PXE44_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL5589730.1"
                     /translation="MKAVKDSVIYLIGELASKSIPFLLLPYFSRKLGVAGYGELSYYQ
                     TFAALFVIFIGLSQDGAVARYFYFYGKRSLNLVLSTGYLYTLCATLLILLGCWYFQSE
                     IIAYVAIGAMFQSFLNVQLSVRQCQKQALAYTTIQLMSAIFSAVITVAMMEYFKTDLV
                     EKRILAILMSNGLVFLIAYFLYAKRAKTKKFSLARHKTALLYLFGFGIPLILHHSSIF
                     LKGQLDRVFIYHKFSEEDLGLYAMGAQIAMILAIVVQALNKATLPYFYEALKQKKINV
                     SHIHKWALYALFIVPLPAIVMWFIPEELVVWILGSQFVGTKYYIILFLIAT"
BASE COUNT         1536 a          822 c         1049 g         1523 t
ORIGIN      
        1 ctaagctaca aaaatataag tgtagaataa ttaatgtggt agataaaacc gcattggtgt
       61 ccaaaaattc cactctaggc attggagtgt ttgttggaaa aatggcaatt gtaaacagtg
      121 gcgtaactgt aggcgataat gtgatcatta acactaaatc gctggttgag catggttgtt
      181 ttatcggcag tcattgcaat atttcaacca atacgacact aaatggcgat gtaattgtcg
      241 aagatcatgc ttttattggt agttcctctg ttgtgaacgg acagctaaga gtgggtgaaa
      301 gtgcattggt tggctccggt gcagtggtaa ttagaaatgt ggagcctcgc acagttgtag
      361 caggcgttcc agcaaaatat attaaggata taaaataatg gtaaatgtat ttattgtggc
      421 agaaatcggt tgtaaccata acggcgatcc tgcgttagca aagaaaatgg ttgatgtagc
      481 gaaagagtgt ggtgtcgatg cggtgaaatt ccaaaccttt aaagcggata agttgatctc
      541 taaatatgcc cctaaagccg agtatcaaaa agtaacgacc ggcaccgctg attcccaatt
      601 agaaatgacc cgcaaattag agttacctta tgaggagttt gtgaaattag aggcttacgc
      661 tcgttcattg gggttagagg tattctctac gccattcgat ttcgagtcga ttgatttctt
      721 ggcaagccaa caacaaaaag tgtggaaaat tccgtctggt gaattaacca atttgccgta
      781 tttagaaaaa attgcacgac ttccgattga aggcaagacg atcgttattt ctaccggtat
      841 ggcaaccatt gaagaaacaa aagcctcatt aaaagtattg gaagataacg gtgtagcgaa
      901 aaaagatatt acgattttac attgtaatac cgagtacccg acaccttatg aagatgtgaa
      961 tttaaatgcg ttccatcagc taaaacagga gtttggcgac tatagtttag gtttttctga
     1021 ccactctgcc ggttattttg cagggttggc ggcagtacct tacggcatta ccttcattga
     1081 aaaacatttt accttagata aaaactttga aggtccggat cataaagctt cggttacacc
     1141 tgaagagctg aaattactgt gtgaaggcat tcgtgcggtg gaaaaatcgc tgggttcttc
     1201 cgagaaatta gtcactaatt cggaagcaaa aaataaaatt gtggcaagaa aatcaattat
     1261 tgcggcacgt ccaattaaaa aaggcgaaat ttttacaacc gaaaatatta cgactaaacg
     1321 ccccggtaat ggcattagcc cgatgttttg gtatgatgtg ctaggtaaag aagcacaaca
     1381 ggattttgaa gaagatcaat taattcaaga ttctcgtttt gaaaatcaga tgtaatataa
     1441 tatagtgatg ggatgaaagc tccatcattt tgtgtttaac aagcggtcaa atttatgaaa
     1501 aaaattgcaa ttattacagc ccgagccggt tcaaaaggct tgccgaataa aaatgtatta
     1561 ttagccaacg gtaagccgtt aatggcctat tctattgaag ctgcgattga gtcggggcaa
     1621 tttgagaaaa ttatcgttag tactgattcc caagaataca ttgatttgtt ctcacattat
     1681 ccaattgaat ttattaaacg tgccgagcat ttagccagtg ataaggcgag ttcttttgtc
     1741 gtgattgaag atgtgttaaa tcaatataga tcagtagagt ttgactattt tatgctactt
     1801 caaccaactt cgccgttgag atcaggagag catattaagg aatcgtgtga aaaatttgag
     1861 aaaaatttcg aacaatttga tttcttagta tctgtttctg atgcccataa acctacgaca
     1921 ttgactcgta tgattgagga agacgagtgc ctaaagcatt ttcagttgga ttattctaac
     1981 tatgcacgcc aattgcatca tcctgaatat tcaccgaatg gagctatttt tacagctaag
     2041 ccagaagcct atcttaaaca aaaacacttc tatggtgcga aatgtctagc ctattttatg
     2101 gataaagacg tttctattga tattgatgat cgccaagatt tcgagtactt ctactttatt
     2161 ctgcaacaac gcaataaaga aaaaatctta cttgaaacca ttaaacgttc gattttaatg
     2221 aaaaaagaga actttaaaga agtgaaagag atagccttaa tcgggcattc gatcattgaa
     2281 tattgggata ttgaacattt aaacggtaag gcagttaata atttaggaat tgcaggtatt
     2341 tcaacaaaac aatacagtga tctgattttg gatgaaggtt taatttctgc tttacctaaa
     2401 gacattattt taatgttcgg tactaatgat attgtatatg ctggctggag tgatgaaaaa
     2461 attctagcag atattaatca tttaattgca caattaacag caattcgtgg agatattaat
     2521 ctctatttct tggaaattac cccaactgca tttagagtcg atcgtaataa caacgatatt
     2581 cgcttgttaa atcaatatct taaagataat ttagcggtga aatggattag cttagatcag
     2641 gcatttagcg ataaatatgg caaattggat ttaagctata gcgatgacgg attacattta
     2701 aatgcaaaag gttatgaaaa attaaccgct attttggaaa aagagctcaa ctaaatgaaa
     2761 aatattgcct atattaccgg atctcgtgcc gaatacggga ttgtaaagcg tttattacaa
     2821 aaattagcac aagatccgac tgtgaatttc tctctggttg ttactgcgat gcatttggat
     2881 ccgcaatatg gcaatacggt gacggtcatt gagcaagatg gctttgagat tgcagctcgt
     2941 attccgctta ccttaaacag tgaaaataat cagaccatta ttcattcaat ggcagagtgt
     3001 ttagatcgat ttggcgaaca tttccagcaa catcgttatg atgcggtgat tgtgctaggc
     3061 gatcgttacg agatgttggc ggtggcaacg gctgcggcga tgcataatat tccgctgatt
     3121 catctgcacg gtggtgagca aacattaggt aattacgatg aatttattcg ccattgtatt
     3181 accaaaatgt cgaaattgca tttaacttca actgaagaat atagacagcg tgtgattcaa
     3241 atgggcgagc tgcccgaaaa tgtggtgaat actggttctc taggggcgga gaatagtttg
     3301 gatttagtgt tgccgaccca agcagaatta gtcgagagat tagggattcc ggcatcgcct
     3361 tattttttag tggtcttcca cccggaaacc ttaaccgatg tttccgtgtt ggagcaaatt
     3421 gaggcgttac tcaatgcttt tgagcagttt aaagacaaat accaattcgt ctttatcggc
     3481 tcaaattcag acaccaattc agagcaaatt tatgcacgtt ttaaggctta caccgatgaa
     3541 aatcaattta agtttttcac ctcagtcaaa ccggaagagt atttagcgtt gattaaatat
     3601 tcaaatgggt tgattggtaa tagctcatca ggcttgattg aagcaccgag ttgtggtgtt
     3661 ggtacgctaa atatcggtaa tcgccagcaa gggcgagtac gggatgagag cgtaattgat
     3721 gtagagagta atgaacaggc tatcatagat ggtatcgaaa aattgatttc agccgatttc
     3781 caacaccgtt taccgagtat gaaaaatcca tattttcaag acaacgcgat ggaaaaggct
     3841 tacaatgcaa tcaaaatatt cttagcagac agccaccata ctcaaccgaa aggattttat
     3901 gatgttgctt tctcgcaagc ggtcaaaaaa tcagaataat ttgcaactat gaaagcagtt
     3961 aaagacagtg tgatttattt aattggggaa ttggcgtcaa agtcaattcc tttcttgctc
     4021 ttaccctact tttcacgcaa actcggcgtg gcaggttatg gggagctttc ctactaccaa
     4081 acctttgctg ctttatttgt aatttttatc gggcttagcc aagacggtgc ggttgctcgc
     4141 tatttctatt tttatggtaa gcgttcacta aatttagtct tgagtacagg gtatttatat
     4201 actctttgtg caactttgct tatcctgttg ggttgctggt atttccagtc tgaaattatt
     4261 gcttatgtag cgattggggc gatgttccag tcgtttttga atgtacagct tagtgtaagg
     4321 cagtgtcaaa aacaggcact tgcttataca acgattcaat taatgtcggc gattttttcg
     4381 gcagttatta ccgttgcaat gatggagtat tttaagaccg atttagtgga aaagcggatt
     4441 ttagcgattt tgatgagtaa tggcttagtc tttctaatcg cttacttttt gtatgctaaa
     4501 agagcaaaaa cgaagaaatt tagccttgcc cgccataaaa ctgctttatt gtatttattc
     4561 ggctttggta ttccgttgat tttgcaccat tccagtattt tcttaaaagg acagttagac
     4621 agagtattta tttaccataa atttagcgaa gaggatttag gtttatatgc aatgggggct
     4681 caaattgcga tgattctagc cattgtggtt caagccttaa ataaagcaac actgccttat
     4741 ttttatgaag ccttaaagca gaaaaaaatt aatgtcagcc atatccacaa atgggcgttg
     4801 tatgccttat ttattgtacc attacctgct attgtgatgt ggtttattcc ggaagaatta
     4861 gtggtatgga ttctgggtag ccaatttgta ggaacaaaat actacattat tctcttttta
     4921 attgcgacaa
//
LOCUS       DANLZC010000158         4788 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.158, whole genome shotgun sequence.
ACCESSION   DANLZC010000158 DANLZC010000000
VERSION     DANLZC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.158"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..561)
                     /locus_tag="PXE44_002264"
     CDS             complement(<1..561)
                     /locus_tag="PXE44_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589731.1"
                     /translation="MEGLIMGLQLIPHFPELAISRRTYLHDMPMYCFVSKNWIFSAKD
                     RAKREALGNLLACGGEMMKSFIFKRSLFSEIWFEDILLEDEEKELIRILYNKPEPEYQ
                     VEEKIDWEAREARRKQADYDKWQSYDERNPFHYQIFPTDTIAYAQQQVEKFIEDVDNF
                     LEWFYDDEVKRYILERNEIYQKKFDLV"
     gene            complement(1094..1420)
                     /locus_tag="PXE44_002265"
     CDS             complement(1094..1420)
                     /locus_tag="PXE44_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5589732.1"
                     /translation="MLHSKGKILPKITLNEIETKERFAIALRKEMHAQYWTGKMVANA
                     LDVSEKTVKDWVAGKRLPSSLDLLALMRISSCVRQLVWDISKSEECYQDAEMLKQICF
                     VLLSNL"
     gene            1468..3099
                     /locus_tag="PXE44_002266"
     CDS             1468..3099
                     /locus_tag="PXE44_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL5589733.1"
                     /translation="MVTRTKNQPKNQAVSYIRMSTEHQEFSPDLQRCFIENYAKENGL
                     EIIREYIDEGKSGLTAEHRPQFLSMIQLVQSGQADFDHILVYDVSRWGRFMNIDESGH
                     YELICTYQGIKVHYCAEPFKGNDIASQIYKTMKRLSVGDYCRELGVKVFNGQKNLIQH
                     GFRQGGVAGFGLRRRLIDCQGNPKFDLQRGDRKSLQTDRVILVAGPKEEQEIVRQIYH
                     DFVYQHKTEQQIADSLNAQGIVTDRNTAWTKGVVHQILINEKYVGHNVWNKRSASKTR
                     EYKGKNPVEEWVRAENVFEAIVPQALFNAAQTIIHQRSARLSDDEMLEQLKLLLKEKG
                     KLSGLIIDEAENCPSSSAYSSRFGSLLNTYTLINYEPERDYHYVEINRLLRQQHKNFV
                     QQTLDTIIELGGVVLSDDVTDLVHINNEFTASIVLSRCRLTPSGSKRWLIRFDTSLNP
                     DITIAVRINESATEILDYYLLPTTEKVNEKLRLAESNPAELEIYRHDNLDRFFIMVER
                     ILVKEFIYAKRHHSNSSYRQNSGTQSQNAQSESLC"
     gene            3014..3907
                     /locus_tag="PXE44_002267"
     CDS             3014..3907
                     /locus_tag="PXE44_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5589734.1"
                     /translation="MQNDTIQILPIDKIRVLNPRTRNQKVFADLVENIATIGLKRPIT
                     VTPSVDSAEYYDLLCGQGRLEACQVLGELFIPCRVVNANSEESYLISLVENIARRQHS
                     YMELLSGIKILNDRGYKPVDIARKIGFSKSHVNSILHLINAGEERLINAVEQGILPIT
                     IAISISRSDDQEVQKQLAELYTNGTLKSTDIAKIRNLIHRRNLAGKKANTGISGSIRS
                     SIYNPKTVVNIYKEETERQKMMIKQADYDEKQLSIILSCLNKLFEDKYFQLVLKSEHL
                     DDMPKDLSQRMLKQREGNIYA"
     gene            3900..4763
                     /locus_tag="PXE44_002268"
     CDS             3900..4763
                     /locus_tag="PXE44_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5589735.1"
                     /translation="MPKHITYAFHNQLISLPIDEIIPLKKMDKNFEKSTKFQSILASI
                     REIGIVEPVVVYPEENNQYILLDGHSRVHALKKLGVDKVLCMISTDDEGFTYNKQINR
                     LTAVQENKMLMKTIERGVSDELIARTLNINLKALRQKMNMLNGITREVVDILANKQVS
                     KEIFRILRKMKPERQIEVAQMMIASNKFTLTYANMMLLSSRKEELVESHKSKTTNDDL
                     TDLSIMQKELERLKENYKISEEKLADLKMGLVVAKGYVKKLLNNAAVVDLLENDQSEI
                     YLTLKEVCDIQ"
BASE COUNT         1573 a          921 c          930 g         1364 t
ORIGIN      
        1 cactaaatcg aatttctttt ggtaaatctc attcctttct aatatatagc gttttacctc
       61 atcatcgtaa aaccattcaa gaaagttatc aacatcttca atgaactttt ctacctgttg
      121 ctgtgcatat gctatcgtat cggtagggaa gatttgataa tgaaacggat tgcgttcatc
      181 ataactctgc catttgtcat aatcggcttg tttacgccgc gcttctcttg cctcccagtc
      241 gattttttct tccacttgat attctggctc tggcttatta tacaaaatac ggatgagttc
      301 cttttcttca tcctcaagca gaatatcttc aaaccagatt tcagaaaata ggctgcgctt
      361 gaaaatgaaa gacttcatca tttcccctcc acacgccaat aagtttccca atgcttcccg
      421 tttcgctcta tcttttgccg aaaatatcca gtttttcgat acaaaacagt acattggcat
      481 gtcatgcaaa taggttcttc gtgaaatggc taattcagga aaatgaggaa taagctgtaa
      541 ccccattatt aagccttcca tactgcacac gatcttactt aattgtccgt aatcgcgtaa
      601 aaaagcaatg tcagttttta tcaaccacaa cgataggctg actaatatga acataaagat
      661 gagataatag atcggtttta aggctaaggc gcgccgacaa agccacatta agagtggaat
      721 cgtaacgaga tcaaagaaaa atgatgagtc tatgatgatt tccttatgtg agttgatgaa
      781 taatcaggca tgaaggaaac cttaaaaagg aaaagtccct tcattaccac tttttaaaac
      841 cttttaaggc ttaaaaatca aaagtcgtaa tgaagggact attgtccgtg ggcaaaaagg
      901 tattagttat agggggcttt gtcaagcgaa aatttactgt gcattttctc gccaaaactg
      961 atttaagcct tgataaaatc tcgaaagtac cttgtcaaga aaaatctttt tagtggtttg
     1021 attgctagtt ttttgagcaa aactatgctg taatgcagta aaaatacagt ttctatctgt
     1081 gtttggaatc cagttacaaa ttggagagta atacaaagca tatttgcttg agcatctcag
     1141 catcttggta gcattcttct gattttgaaa tatcccaaac aagctgccgt acacatgatg
     1201 aaattctcat taacgccaat aaatctaggc tacttggcaa tcttttccct gcaacccaat
     1261 ctttaactgt tttctcactc acatctaatg cattggcgac cattttcccc gtccaatatt
     1321 gagcatgcat ttcctttcgt aacgcaattg caaaccgctc tttcgtttca atctcattga
     1381 gtgtaatttt gggtaagatt ttaccctttg agtgcaacat atttcctccc gattccttta
     1441 tattggtgta gataaggagt cattagaatg gttacacgta caaagaatca acctaaaaat
     1501 caggcggttt cctacattcg aatgtcaacg gaacaccaag aattttcacc agaccttcaa
     1561 cgttgcttta tcgaaaatta tgctaaagaa aatgggctag aaatcattag agaatacatt
     1621 gatgaaggaa aaagtgggct aaccgcagaa caccgcccac aattcctttc aatgatacaa
     1681 ctcgtacaaa gtggacaagc cgacttcgat catattttgg tgtatgacgt gagtcgttgg
     1741 ggtcggttta tgaatattga tgaatcgggg cattatgaac tgatttgtac ctaccaaggc
     1801 attaaagtcc actattgcgc cgagccattc aaagggaatg atattgcctc tcaaatatat
     1861 aaaaccatga aacgtttgtc ggtaggagac tactgtcgtg aactcggagt gaaagtattt
     1921 aacggtcaaa aaaatctgat ccaacatgga tttagacaag gtggggtagc tggatttgga
     1981 ttgcgtagga gattgattga ctgtcaagga aaccctaaat ttgatctcca acgtggagat
     2041 agaaagagcc tacaaaccga cagagtgatt ttggttgcag gcccaaagga agagcaggaa
     2101 attgtccgac aaatttacca tgattttgtt tatcaacata aaacggaaca acaaattgct
     2161 gatagcttaa atgcacaagg cattgttact gatagaaata cagcatggac gaaaggcgta
     2221 gtacatcaaa tccttattaa cgaaaagtat gtcggccaca atgtttggaa taaacgtagt
     2281 gcatcaaaaa cacgcgaata taaaggtaaa aaccccgtag aagagtgggt gcgagctgag
     2341 aatgtatttg aagctatcgt gccacaagcc ttatttaatg cagcccaaac cattattcat
     2401 caacgctccg cccgattatc tgacgatgaa atgttagagc aactcaagct acttttgaaa
     2461 gagaaaggga agctgtctgg cttgatcatt gatgaagccg aaaattgtcc atcaagtagc
     2521 gcgtacagta gccgatttgg aagcctactt aacacatata ctttaattaa ctatgaaccc
     2581 gaacgggatt accactatgt agagatcaac cgattactcc gtcagcagca taaaaatttc
     2641 gtacaacaaa cacttgatac cattattgaa ttggggggag tggttttgag cgatgatgta
     2701 accgatttag tccacatcaa taatgaattt accgcctcaa tcgtattaag tcgttgtcgc
     2761 ctaacgccat caggaagcaa aagatggttg atccgatttg ataccagttt aaatcccgac
     2821 ataacaattg cagtgcgcat caatgagagt gccacagaaa ttcttgatta ctacctgctt
     2881 cccacaactg aaaaggtaaa tgaaaaacta cgattggctg agagtaatcc tgccgaactt
     2941 gaaatctacc gccatgataa tttagatcgc ttttttatta tggttgaacg tattttagtc
     3001 aaggagttta tctatgcaaa acgacaccat tcaaattctt cctatcgaca aaattcgggt
     3061 actcaatccc agaacgcgca atcagaaagt ctttgctgat ctggtcgaaa acattgcaac
     3121 gattggtctc aagagaccta ttaccgtaac gccatctgtc gatagtgctg aatattatga
     3181 tttgttatgc ggtcaaggtc ggcttgaggc ttgtcaggtg cttggtgagc tattcatacc
     3241 ttgtcgcgtt gtgaacgcaa atagtgaaga aagctacttg atcagccttg tggaaaatat
     3301 tgccagacgt cagcattcct atatggagct gctttcaggc atcaaaatac tcaatgatag
     3361 aggttataag cccgttgata ttgctcgcaa aatcggtttc tcaaaaagcc atgtcaacag
     3421 tatcttacac ctcatcaatg ctggggaaga gcgccttatc aatgcggtag agcaaggaat
     3481 tttacccatt accatcgcta tttcgatttc tcgttcagac gatcaggaag ttcaaaaaca
     3541 actcgcggaa ctttacacca atggtacatt gaaaagcacc gatattgcca aaatacgtaa
     3601 cctgatccac cgtagaaatc ttgctggtaa gaaagccaat acaggtattt ctggaagtat
     3661 tagatcatct atttataatc ccaaaactgt ggtgaatatc tacaaagagg aaacggaaag
     3721 acagaaaatg atgatcaagc aagcggacta tgatgaaaaa caactctcta ttatcttgtc
     3781 ttgcttaaat aaactctttg aggacaaata ctttcaactc gtccttaaat cagaacattt
     3841 agacgatatg ccaaaagatt taagccaacg aatgttaaaa caacgagagg gaaatattta
     3901 tgcctaaaca tattacttat gcctttcata atcagttaat ttcgcttcca attgatgaga
     3961 ttattccatt aaaaaagatg gataaaaatt ttgagaaaag cactaagttc caaagcattc
     4021 tggcttcaat tcgtgaaatc gggattgttg aacctgtggt cgtctatcca gaagaaaaca
     4081 accaatatat tttgttagat ggccattctc gtgtacatgc actcaaaaag ctaggtgtgg
     4141 ataaggtgct ttgtatgatt tctaccgatg atgaaggctt tacttacaac aagcaaatta
     4201 accgcttaac cgccgttcag gaaaataaaa tgctgatgaa aaccattgag cgcggggttt
     4261 cggatgaact gatcgcaaga acactcaata tcaatctaaa agcactacgg cagaaaatga
     4321 atatgcttaa tgggatcaca agagaggttg ttgatatatt ggccaataag caggtcagta
     4381 aagagatatt tcggattttg cgcaagatga agcctgaacg ccaaattgaa gtggcacaaa
     4441 tgatgatcgc cagtaataag tttacattga catacgccaa tatgatgttg ctcagttcac
     4501 gcaaggagga attagtcgaa agccacaagt ctaaaaccac gaatgatgat ttaaccgatc
     4561 tttccattat gcaaaaagag ctagagcgac ttaaagagaa ctataaaatt tccgaagaga
     4621 aattggcgga cttaaaaatg ggattggttg tggcgaaagg gtatgtcaaa aaactattga
     4681 ataatgctgc cgtggtagat ttgcttgaaa atgatcagag tgaaatctat cttactctta
     4741 aagaagtatg tgatattcaa taatataata tctagctaat aaccttaa
//
LOCUS       DANLZC010000159         4753 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.159, whole genome shotgun sequence.
ACCESSION   DANLZC010000159 DANLZC010000000
VERSION     DANLZC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.159"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(27..737)
                     /locus_tag="PXE44_002269"
     CDS             complement(27..737)
                     /locus_tag="PXE44_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HDL5589736.1"
                     /translation="MFMVIQLALYNLQTYEVGRNATTIFMTTDKVYTGGSTWTYLFAD
                     ADNKITSVREMNGFGDVEKEFKCGEYKEAVTIASKEDLAKRKTQPRLWSSIYMSPEQN
                     MMWVTDNTSKNLENAKQDAFKQCEKNGGRNCQMILSFSNMCLAFAQGYKDGQGFEDFG
                     YGIVSSLAEENALENCNQKGGSSCLLSAPAVCAIPCDILSNNKCMYDQPQIIIPGKNN
                     NQPINPPANGNGEHWLSN"
     gene            complement(706..852)
                     /locus_tag="PXE44_002270"
     CDS             complement(706..852)
                     /locus_tag="PXE44_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589737.1"
                     /translation="MIKPLDSVYWRGKDVSEFFARKRLPDTAVQCVKDNELSYVYGYS
                     TSFV"
     gene            complement(970..2187)
                     /gene="fabB"
                     /locus_tag="PXE44_002271"
     CDS             complement(970..2187)
                     /gene="fabB"
                     /locus_tag="PXE44_002271"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDL5589738.1"
                     /translation="MKRVVITGLGVISSIGNNKEEVLESLKAGKSGIEFVPEFAEVGM
                     RSQVAGTIKLNPAELIDRKIYRFMGDAAAYAYLSMKEAIEDSGLTEEQVSNDRTGLVI
                     GAGTGSAHSQLVACDAVRGPRGVKAVGPYAVTKTMASSVSACLATPYKIRGVNYSISS
                     ACATSAHCIGHAMELIQLGKQDVVFAGGAEELSWECATEFDAMGAVSTKYNETPTKAS
                     RAYDANRDGFVIAGGGAVVVVEELEHALARGAKIYAEIVGYGATSDGYDMVAPSGEGA
                     ERCMRQAMATVNGEIEYINVHGTSTPVGDVKELGAIRNVFGDKTPAISSTKSMTGHSL
                     GAAGAHEAIYSLLMLDNNFIAPSINIETLDEQAQGMNIVTERQDKALTTVMSNSFGFG
                     GTNACLVFQRYSK"
     gene            2448..>4753
                     /locus_tag="PXE44_002272"
     CDS             2448..>4753
                     /locus_tag="PXE44_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL5589739.1"
                     /translation="MKTLTLTANKITAVEGAAIGANQGSFAQKLNSTKARIDQLNAYR
                     IERAINANNHHFMHVFSLIPLLIHLNHPVLPAYVENAPQGIWHFQLSDYQKQFLVSYR
                     FDEAISADHLGPHPSFDALYSMGSTGTITQTSCSDLDLWLCYPNHFTIEQYKLMELKI
                     NKLQEWAKTFQIDINIYLMNPEQFKSQTYNSSVTDEHSGSAQHFFLLDEFYRSAILLA
                     GKRLLWLHLHKNEYQNVHNNPEIDLTEWIDFGDFSSLSTSEFFGASLWQLYKGINNPY
                     KSAIKILLLECYAHTYPKTKLISKEFKKKLLSDNALEYHFDPYLAMLELVTEHLRSRK
                     EWVKLDRLRVCFYTKAVEGEINNNWRSRDLDELVNQWSWSEKQIQLLQNRQHWKIKQV
                     MNHENMLIDHLLQSYRNLIHFARKFHINPSIMTNDTDILMRKLYSVFEALPGKISLIN
                     HKISSNLAEKAITFIEAKVGSALQPGWYLVNQAPKSRYDSENRYVHYNKSPIKLIAWG
                     YFNGIINAATNLHTVSPNLDIEKLRQFITDLRLSFPIQAPEVTNDDMHHPNEIRNLVI
                     ALNLVQDPTEHIDELPKKVYPSDLFNFGKAQKTLVGSVSIIYRNVWNEIRTQHFDGED
                     AILKALKLVSNRIYQGNSSPQSVNVFCYSKYLRSELRDFVADLVHKCITIQTGCLLQE
                     NEFSTLKVAGKTWQFLFHKKTFSIKQIDEQQAVENLNSFAKTKQNAKSENEKKKEFPQ
                     QISEFASEGFLQFFFEDNVDNSFNVYVV"
BASE COUNT         1525 a          969 c          781 g         1478 t
ORIGIN      
        1 gtttccgcac tttttactaa aatgatttaa ttactcaacc aatgctcacc gttgccatta
       61 gctggtggat taatcggttg gttattattc ttacctggaa taataatttg aggctgatcg
      121 tacatacatt tgttgttcga caatatatca cagggaatag cacatacggc aggggcagaa
      181 agaagacaac ttgaaccacc tttttgatta caattctcta atgcattttc ttcagcaaga
      241 ctactaacga taccataccc aaaatcctcg aagccttgac catctttata accttgtgca
      301 aaagcaaggc acatattact aaacgataag atcatttggc aattacgacc accgttcttt
      361 tcacactgtt taaacgcatc ttgcttcgca ttttctaaat ttttagacgt attatcagtc
      421 acccacatca tattttgttc aggtgacata taaattgatg accataaacg aggctgtgtt
      481 ttacgctttg ctaagtcctc tttactggca atggttaccg cttctttata ttcaccacat
      541 ttaaactctt tttcgacatc accaaaacca ttcatttcac gaacagaagt gattttatta
      601 tcagcgtcag caaaaagata tgtccaagtc gatccacctg tataaacctt atctgttgtc
      661 ataaatatag tggttgcatt acgaccaact tcataagttt gcaaattata caaagctagt
      721 tgaataacca taaacatagc ttaattcatt atcttttacg cattgaaccg ctgtatcagg
      781 taaacgttta cgtgcaaaaa attcagatac atctttacca cgccaataaa cactatctag
      841 tggcttaatc attgtagaac aagcggtcaa aaataatgac aaaataacaa atagcaaaga
      901 acttattttg aaatttataa taattacctc aaaagaaaaa agagcaagct ttcacttgct
      961 ctttttatgt tacttagaat agcgttggaa cactaaacac gcattagtac caccaaagcc
     1021 gaagctgttt gacatcacgg ttgttaatgc tttgtcttgg cgttcagtca caatattcat
     1081 accttgtgct tgttcatcaa gggtttcaat attaatgctt ggtgcaataa aattattatc
     1141 taacattaat aatgaataaa ttgcctcgtg cgcacctgct gcacctaatg agtgtccggt
     1201 cattgatttg gttgatgaaa ttgctggcgt tttatcacca aagacattac ggatcgcccc
     1261 taactctttt acatcaccca ctggagtaga agtgccgtgt acgttaatat actcgatttc
     1321 accatttaca gttgccatcg cctgtctcat acaacgctca gcaccttcac cacttggcgc
     1381 gaccatatcg taaccgtctg aggttgcccc atagcccaca atttcagcat agattttggc
     1441 accacgagca agagcgtgtt ctaactcttc caccacaacc actgcaccac caccggcaat
     1501 cacaaaaccg tcacgattag catcataagc acggctagct tttgttggtg tttcattata
     1561 tttagttgaa acagcaccca ttgcatcaaa ctcggttgca cattcccaag aaagttcttc
     1621 tgcaccacct gcaaacacca catcttgttt acctaattgg attaactcca tcgcgtgtcc
     1681 aatacagtgt gctgaagtcg cacaagctga gctgattgaa tagtttacac cacgaatttt
     1741 ataaggtgtt gccaagcatg ctgacacact tgatgccatt gttttggtta ctgcgtaagg
     1801 cccaacggct ttcacaccac gtgggccacg tactgcatca caagctacaa gttgactgtg
     1861 cgccgagcct gtaccagctc cgatcaccaa accggtacga tcgtttgaaa cttgttcttc
     1921 ggttaagcct gagtcttcga ttgcttcttt cattgaaaga taagcgtaag ccgcagcatc
     1981 tcccataaaa cgatagattt tacggtcaat taactcagcc ggatttaatt taatcgtacc
     2041 tgccacttgg ctacgcatac ccacttcagc aaactcaggc acaaattcaa tgcctgattt
     2101 acctgctttt agtgattcca acacttcttc tttgttgtta ccgatactgg aaattacacc
     2161 taatcctgta ataacgactc ttttcatttt gtttccttca tcattttgat ctgaaaaata
     2221 cttgattcag cttacacttg ttcactcaaa taagctacta aaacttgatt attttacaca
     2281 atttcattac tggtctgaac tctttctatc aagatagcgg ttgaatttca gacatttttt
     2341 gcaaaatagg gaatgaaatt taatttcccg attttttacc ctattacata ataatcactt
     2401 ttccgctaaa atgctaaaca gttcacactt tagtaaggaa agctgacgtg aaaacattaa
     2461 ccctgacagc caacaaaata acagccgtag aaggcgctgc cattggggcg aaccagggga
     2521 gcttcgccca aaaattaaac tctactaaag cacgaattga tcaacttaat gcataccgaa
     2581 tagaacgagc tatcaatgct aataatcatc attttatgca tgttttctct ctcattccat
     2641 tgcttattca tcttaatcac cctgtgttac cggcttatgt tgaaaatgca ccacaaggga
     2701 tttggcattt tcaactctct gattatcaaa aacaatttct ggtttcttac cgttttgatg
     2761 aagcaatctc tgccgatcat ttgggccccc atcccagctt tgatgctctt tattcgatgg
     2821 gtagcaccgg tactattacc caaacaagct gttctgattt agatttatgg ctatgctatc
     2881 cgaaccattt tacaattgaa caatataagt taatggagct caaaattaat aaattgcaag
     2941 aatgggcaaa gacattccaa atcgacatca atatttattt aatgaaccca gaacaattta
     3001 agagccaaac ttataacagt agcgtaaccg atgaacacag tggttcagca cagcattttt
     3061 ttctattaga tgaattttat cgttcagcta ttttacttgc cggtaagcgt ttactctggt
     3121 tgcatttaca taaaaatgaa taccaaaatg ttcacaataa ccctgaaatt gacttaacgg
     3181 aatggattga tttcggcgat ttttccagcc tttcaaccag tgaatttttt ggtgcaagct
     3241 tatggcagct ctacaaagga attaataacc cttataaatc ggcaattaaa attttattgc
     3301 tcgaatgcta cgctcacact tatcctaaaa caaagctcat ctccaaagaa tttaagaaaa
     3361 aactactaag tgacaatgct ttggaatatc actttgatcc ctacttagcc atgcttgaat
     3421 tggtaacaga acacttaaga tcacgcaaag agtgggtgaa attagaccgc ttgcgagtct
     3481 gtttttatac aaaagcggtt gagggagaaa taaataacaa ctggagaagt cgtgatttag
     3541 acgagctggt taaccaatgg tcttggtcag aaaaacaaat tcaattactt caaaaccgtc
     3601 aacattggaa aattaagcaa gtgatgaatc atgagaatat gctgattgat catttactac
     3661 aaagttatcg aaatttaatt cattttgctc gaaaatttca tattaatccg agcattatga
     3721 caaatgatac tgatatttta atgcgaaaac tttattctgt atttgaagca cttccaggaa
     3781 aaatatcttt aattaatcat aaaatatcgt ccaatttagc agaaaaagca attacattta
     3841 ttgaagctaa agttggatct gcgttacaac ccggttggta tttagttaat caggcaccta
     3901 aaagtcgtta tgactctgaa aatcgttatg tccattataa taaaagcccg attaagctta
     3961 ttgcttgggg gtattttaac ggcattatta acgcagcaac taatttacat actgttagcc
     4021 ctaatttaga tatcgagaaa ttgcgacaat ttatcaccga cttgcgctta tctttcccaa
     4081 ttcaagcgcc ggaagtgacc aatgatgaca tgcatcaccc taatgaaatt cgcaatttgg
     4141 ttatagcgct taatttagta caagatccaa ccgaacatat cgatgaacta ccaaaaaaag
     4201 tttatccaag cgatctgttt aattttggta aggcacaaaa aacgttagtt ggtagtgtga
     4261 gcattattta tcgaaatgta tggaatgaaa ttcgaactca acattttgac ggtgaagatg
     4321 ctattttaaa agcactaaaa ttggtatcca atcgaattta tcaaggaaat tcttcacctc
     4381 aatcagttaa tgttttttgc tatagtaaat atctacgtag tgagttacgt gattttgtgg
     4441 cagatttagt tcataaatgt attacaattc agactggttg tttactgcaa gagaatgaat
     4501 ttagcacttt aaaagtagca ggaaaaacct ggcagtttct ttttcataaa aaaacattta
     4561 gcattaaaca aattgatgaa caacaagcgg ttgaaaattt aaattctttt gcaaaaacca
     4621 agcaaaatgc taaaagcgaa aatgaaaaga aaaaagaatt tccgcagcaa attagtgaat
     4681 ttgccagtga aggcttttta cagtttttct ttgaagacaa tgtcgataat agttttaatg
     4741 tatatgttgt aga
//
LOCUS       DANLZC010000160         4611 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.160, whole genome shotgun sequence.
ACCESSION   DANLZC010000160 DANLZC010000000
VERSION     DANLZC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.160"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            106..4002
                     /gene="purL"
                     /locus_tag="PXE44_002273"
                     /gene_synonym="purI"
     CDS             106..4002
                     /gene="purL"
                     /locus_tag="PXE44_002273"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDL5589740.1"
                     /translation="MTIQTFRGSPALSAFRIQQLLQKFQQNQLPVKSVYAEFLHFVAL
                     NRPLVADEENKIKELLHYGPTLEEDEPTGFCLIVTPRIGTISSWSSKATDIAHNCGLA
                     AVERIERGTAYYLEFSETPNESQLTTLKGLLHDRMMETVLDNESQAEQLFAQQEPKPF
                     TSVDILGGGRKALEEANINLGLALADDEIDYLVENFTQLGRNPNDIELYMFAQANSEH
                     CRHKIFNADWTIDGVKQEKSLFKMIKNTFEKTPDYVLSAYKDNAAVMEGSTVGRFFPD
                     QDGQYRYHQEDAHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLT
                     GFSVSNLVIPKFEQPWENPLSKPNRIASALDIMIDGPLGGAAFNNEFGRPALLGYFRT
                     YEEKVNSFAGEEIRGYHKPIMLAGGIGNIRAEHVQKGEIPVGAKLIVLGGPAMNIGLG
                     GGAASSMASGKSKEDLDFASVQRENPEMERRCQEVIDRCWQMGAENPILFIHDVGAGG
                     LSNAMPELVHDGECGGKFDLRNILCDEKGMSPLEIWCNESQERYVLAVSPEKLDIFTA
                     LCERERAPFAVIGEATKEKHLTLADPHFDNKPIDLPMNVLLGKTPKMQRDVLSKTVKN
                     PPLATADIDLKEAFHRVLRLPAVAEKTFLITIGDRSVTGMVAQDQMVGPWQIPVADCA
                     VTTASLDSYHGEAMSIGERSPVALLDFAASARLAVAESITNIAATNIGDIKRIKLSAN
                     WMSAAGHEGEDAGLYEAVKAVGEELCPELGITIPVGKDSMSMKTTWEENGEQKSVTAP
                     LSLVISAFARVEDVRKTVTPQLRTDKGDTRLLLIDLGEGNNRLGATALAQVYKQLGDK
                     PADVVNVARLKGFFNAMQTLVAQGKLLAYHDRSDGGLITTLAEMAFAGNCGISVEIDR
                     LVDDLAVLFNEELGAVIQVRTEELSEVQAVLNAENLANLTFDIGTAVESDSFEITRNG
                     TTVFSQKRSELRGIWAELTHQMQRLRDNPECADQEFAAKKDPNNKGLSAFLTYDVNED
                     IAAPYINKGVKPSVAILREQGVNSHYEMAAVFDRAGFNAIDVHMSDLISGRRNLNDFN
                     AMVACGGFSYGDVLGAGGGWAKSILFNPMLRDQFSKFFANPNTLALGVCNGCQMVSNL
                     AEIIPGTENWPRFVRNKSERFEARAAMVKINDTNSLWFNGMAGSHMPIAVSHGEGQVE
                     FKNPQQLANLQQQNLVIAQYVDSHLNPTEQYPANPNGSALGITAIGNTDGRVAIMMPH
                     PERVFRAVSNSWCPEDWTEGGAWMRLFRNARVALKY"
     gene            4357..4539
                     /locus_tag="PXE44_002274"
                     /pseudo
     CDS             4357..4539
                     /locus_tag="PXE44_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1288 a         1045 c         1128 g         1150 t
ORIGIN      
        1 atgaataaat gtatctaaca gcaaacgtct gacaaatata gcaatcgttt gcgcaatcgt
       61 ttataatcgg tttagttttt catcttaaat aggaaaatcc aaagaatgac aatccaaacc
      121 ttccgtggct cgcctgcctt atctgctttt cgcattcaac aacttctgca aaaattccaa
      181 caaaatcaat tacctgtaaa atcagtttat gcggagtttt tgcattttgt ggcattaaat
      241 cgaccgcttg tagccgatga agaaaacaaa atcaaagagt tattgcacta cggtccaacg
      301 ctggaagaag acgagccgac agggttttgc ctgattgtta cccctcgtat cggcacgatt
      361 tcctcgtggt cgtctaaggc aaccgacatc gcccataact gcggtttggc agcagtagag
      421 cgaattgagc gtggcacggc atattattta gaattttctg aaacgccgaa cgaaagtcaa
      481 cttacgacgt tgaaaggctt attacacgac cgtatgatgg aaacggtgct ggataacgaa
      541 agccaagccg aacagctttt cgctcagcaa gagccgaagc cgtttacttc ggtggatatt
      601 ttaggcggtg gtcgcaaggc actggaagaa gcgaatatca acttaggttt agcgttggcg
      661 gacgatgaaa ttgattactt ggtggaaaac tttacccaac ttgggcgtaa ccccaatgat
      721 attgaactct atatgttcgc ccaagccaac tcggagcatt gccgccacaa aatttttaat
      781 gcggattgga cgattgatgg ggtaaaacag gaaaaatccc tgtttaagat gattaaaaac
      841 acctttgaga aaaccccgga ctatgtgctt tccgcctaca aagacaatgc ggcggtgatg
      901 gagggctcaa cggttgggcg tttcttcccc gaccaagatg gacaatatcg ctatcatcaa
      961 gaagatgctc atattttaat gaaagtagaa actcacaacc acccaaccgc catctcgcca
     1021 ttcccgggag cggcgaccgg ttcgggcggt gaaattcgtg atgaaggggc gacagggcgt
     1081 ggggcgaaac cgaaagcggg cttgacgggt ttttcggtct ctaacttagt gatcccaaaa
     1141 tttgaacagc cgtgggaaaa cccgctttcc aagccgaatc gcattgcctc cgcactggat
     1201 attatgattg atggcccact aggcggagcg gcatttaaca atgaatttgg tcgtcctgcg
     1261 ttattgggct acttccgtac ttacgaagaa aaagtgaaca gcttcgcagg cgaagaaatt
     1321 cgtggctatc acaagccgat tatgttagca ggcggtatcg gcaacattcg tgccgaacac
     1381 gttcaaaaag gcgaaatccc ggtgggagcg aagttgatcg tgcttggtgg cccggcaatg
     1441 aacatcggct taggcggtgg ggcggcatcg tcaatggcat cggggaaatc gaaagaagat
     1501 ttggattttg cctcggttca gcgggaaaac cctgaaatgg aacgccgttg ccaagaggtg
     1561 atcgaccgtt gctggcagat gggggcggag aacccaattt tatttattca cgacgtgggg
     1621 gctggcggtt tatcaaatgc gatgcctgaa ttggttcacg acggcgaatg tggcggtaag
     1681 tttgatttgc gtaacatctt gtgcgatgaa aaaggaatgt cgccacttga aatttggtgt
     1741 aacgaatcgc aagagcgtta tgtgcttgcc gtttcccctg aaaaactcga cattttcaca
     1801 gcattgtgcg aaagggaaag agccccattt gcggtgattg gcgaggcaac gaaagagaag
     1861 catttaaccc ttgctgaccc tcattttgac aataaaccga ttgatttgcc gatgaatgtg
     1921 ttgctcggca aaacgccaaa aatgcagcga gatgttttgt caaaaactgt gaaaaatcca
     1981 ccgcttgcaa ccgctgacat cgacttgaaa gaggcgttcc accgtgtgct acgcttgcct
     2041 gcggtggctg aaaaaacctt cctgattacc atcggcgatc gctcagtaac gggaatggtg
     2101 gcacaagatc agatggtcgg cccgtggcaa attcctgtgg cagattgtgc cgtaaccacc
     2161 gcttcattag acagctatca cggtgaagca atgtcaattg gcgagcgatc acctgttgct
     2221 ttgcttgact ttgctgcctc agctcgcctc gccgtggcag aaagcatcac caatattgcc
     2281 gccactaaca ttggcgacat caaacgcatt aaactctcgg cgaattggat gtcggcggca
     2341 gggcacgagg gcgaagacgc agggctttac gaggcagtaa aagcggtggg cgaagagctt
     2401 tgccctgaat taggcataac cattccggta ggcaaagact caatgtcgat gaaaaccact
     2461 tgggaggaaa acggcgaaca gaaatctgtt actgctccgc tttcgctcgt gatttccgct
     2521 ttcgctcgtg tggaagatgt gcgtaaaacc gttaccccac aacttcgcac cgacaaaggc
     2581 gatacccgct tgttgctgat cgacctgggc gaaggcaaca accgcttggg tgcaaccgca
     2641 cttgctcaag tttacaaaca acttggcgac aaacctgccg atgtggtgaa tgtggctcgc
     2701 ttaaaaggct tcttcaacgc aatgcaaacc cttgtcgcac aaggaaaatt gcttgcctac
     2761 cacgaccgct cggacggtgg tttaatcacc acactggcgg aaatggcgtt tgcagggaat
     2821 tgcggaattt cggtggaaat cgaccgcttg gtggacgatt tagccgtgtt attcaacgaa
     2881 gaactggggg cagtaatcca agttcgcacc gaagaattga gcgaggtgca agcggtgtta
     2941 aatgccgaaa atcttgcaaa tctcaccttt gatattggta cagcggttga aagcgacagc
     3001 tttgaaatta cccgcaacgg cacaacagta tttagccaaa aacgttccga actgcgtggc
     3061 atttgggcgg aactcactca tcaaatgcag cgtttgcgag acaaccctga atgtgcagac
     3121 caagaatttg cagcgaaaaa agacccgaat aacaaaggct tatccgcatt tttaacttac
     3181 gatgtgaacg aagacatcgc cgccccatac atcaacaaag gcgtaaaacc gagtgtggcg
     3241 attttgcgtg agcaaggtgt aaacagccac tacgaaatgg cagccgtctt tgaccgtgcc
     3301 ggtttcaacg ccattgacgt gcatatgtca gacttgattt caggcagacg caacctcaac
     3361 gacttcaacg caatggtggc gtgcggaggc ttctcttacg gcgatgtctt aggtgcaggc
     3421 ggcggctggg cgaaatcgat tctgttcaac ccaatgctgc gtgaccaatt cagcaaattc
     3481 ttcgccaacc cgaacacgct ggcattaggg gtatgtaatg gttgccaaat ggtctccaat
     3541 ctagcggaaa ttattccagg cacagaaaac tggccacgct tcgtgcgtaa taaatccgaa
     3601 cgttttgaag cccgtgctgc aatggtgaaa atcaacgaca ccaactcact ttggttcaac
     3661 ggaatggcag gcagccatat gccgattgcg gtttcacacg gtgaaggaca agttgagttc
     3721 aaaaatccac agcaattagc gaacttgcaa cagcaaaatc tcgtgatcgc acaatatgtg
     3781 gatagccacc tcaacccaac cgaacaatac ccagccaacc cgaacggatc ggcgttgggt
     3841 atcaccgcta tcggtaatac agacggacga gtcgctatta tgatgccaca ccctgaacgt
     3901 gtgttccgtg cggtcagtaa ctcttggtgc ccagaagatt ggacggaagg tggagcgtgg
     3961 atgcggttgt ttagaaatgc gagagtggcg ttgaagtatt aaaaatctac cttcgtagtg
     4021 atctgccccc atagggggcg attaaaattc gaattcatag aacccgaatt tttatatatc
     4081 tgataaacga agttgtttat ccttcgtgct tttcctgata tttcaagtac tttcttatca
     4141 tttctgcatt gacatctacg gtgtctacgc agtagcctct tgccacaaaa gtgggttgcc
     4201 ccacaattta tgcttacgta aatagggaaa cttattgaaa aatcttattg gaccctgttc
     4261 acccttttaa atgccccatt acgtcttata cagaaaatga acgtgattta tttgaacggt
     4321 caattctacc ttaatagaaa gtggttttta gttagcaatc aaaatcacat taacggaatt
     4381 gagaatttgg agagtcaggc aaaatgctat ttgcgtaaat ttaacggcgt tcctaaagta
     4441 catgaattct atttgaaaga atgcagatgg cgttttaatc acagtaactt aaaatatcaa
     4501 gttttctttt taaaacaatt ggttaaattg agtttctagg atagcccctt atttttataa
     4561 tcctctattt aagtgcatat ttctatttat gctaatcgtc taacgtatcc t
//
LOCUS       DANLZC010000161         4558 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.161, whole genome shotgun sequence.
ACCESSION   DANLZC010000161 DANLZC010000000
VERSION     DANLZC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.161"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..315
                     /gene="trxA"
                     /locus_tag="PXE44_002275"
     CDS             <1..315
                     /gene="trxA"
                     /locus_tag="PXE44_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL5589741.1"
                     /translation="HTTDATFEQDVLKSDVPVLLDFWAPWCGPCRMIGPVLDDLSVDA
                     QFDGKVKIVKMNVDENPSVPAQFGVRSIPFLLLFKNGEVIAQQVGAIPHAQMAEFIKQ
                     AL"
     gene            486..1709
                     /gene="hflK"
                     /locus_tag="PXE44_002276"
     CDS             486..1709
                     /gene="hflK"
                     /locus_tag="PXE44_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDL5589742.1"
                     /translation="MSWNESGNQQDPWGKPGQKKAEPKPEQQEQGSQQEPTNNQRNEQ
                     QPPDLEEVFSSLLKKMGGGKGPNNSSNQPSASLGKFLPVVLGLAAVVWVGSGFYTVQE
                     AERGVVTRLGKLNDIVLPGLNWKPTFIDSVTRVNVERVSELNTSGSMLTQDENMVQVE
                     MTVQYRVEDPAKYLFSVSNPDDSLKQATDSALRYVIGHMTMDEILTTGRATVRERTWS
                     TLRDIIKTYDMGLLVTDVNFQYARPPEEVKAAFDDAIKAQEDEQRLIREAEAYARGEE
                     PIARGQAQRTIEQAQAYKEAVVLNAKGEVERLSQLLPEYKASPELTRERLYIQTMEKV
                     MKNTPKVVMDSSGNNLNVLPFDKLMNSSSVIKAEQPAAAQVVTPQVVQPQVQPRTQIQ
                     PQQAEQQEPTRKGRF"
     gene            1712..2599
                     /gene="hflC"
                     /locus_tag="PXE44_002277"
     CDS             1712..2599
                     /gene="hflC"
                     /locus_tag="PXE44_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDL5589743.1"
                     /translation="MRRLLVPILAVVAFVVLQAITIVNEGERGIMLRFNKVHRDSDQK
                     VVVYEPGIHFKVPFIDSLKVLDARIQTLDGQEDRFVTVEKKDLLVDSYVKWRISDFGQ
                     FYTSTGGDYQKAADLLRRKVGDRLRSEIGSRTIKDIVSGSRGELMAGAQKALNAGEDG
                     AERLGIEVVDVRVKQINLPNEVSSSIYQRMRAERDAVAREHRSQGNEKAEVIRAEVDK
                     KVVLILANANKTAQALRGEGDAQAAKLYSEKFGNEPEFYSFIRSLKAYEDSFAEGQNN
                     MMLLKPNSEFLRFMQAPTK"
     gene            2773..4071
                     /gene="purA"
                     /locus_tag="PXE44_002278"
     CDS             2773..4071
                     /gene="purA"
                     /locus_tag="PXE44_002278"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL5589744.1"
                     /translation="MGKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTL
                     IINGEKTVLRLIPSGILRDNVTCLIGNGVVLSPEALMKEMGELEERGINVRERLKISE
                     ACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEHFAA
                     KLKDILDYYNFQLVNYYKVEAVDYQKTLDDVFAIADVITGMVADVTTLLHEARENGDN
                     ILFEGAQGTMLDIDHGTYPFVTSSNTTAGGVATGSGYGPRHLDYVLGIIKAYCTRVGS
                     GPFTTELFDDVGAEIARKGNEFGAVTGRPRRCGWFDAVAVRRAVQINSISGFCMTKLD
                     VLDGFKELKICVAYKMPNGKIVEYAPLAAKDWEGVEPIYETMPGWSENTFRVTKREDL
                     PQAALDYIKRIEELVGVPVDILSTGPDRVETMILRDPFAA"
     gene            complement(4231..4301)
                     /locus_tag="PXE44_002279"
     tRNA            complement(4231..4301)
                     /locus_tag="PXE44_002279"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4271..4273),aa:Arg,seq:tct)
BASE COUNT         1407 a          819 c         1054 g         1278 t
ORIGIN      
        1 catacaacag atgctacatt tgaacaagat gttttaaaat cagatgtacc ggtattatta
       61 gacttctggg cgccttggtg tggtccttgc cgtatgatcg gccccgtact tgacgattta
      121 tctgttgatg cacaatttga tggtaaagta aaaattgtca aaatgaacgt agatgaaaat
      181 ccatctgtac cagcacaatt tggcgtacgc agcattccat ttttactatt attcaaaaat
      241 ggtgaagtga ttgcccagca agttggtgca attccgcacg cccaaatggc tgaatttatc
      301 aaacaagcat tataatttta agagcaatac tagggcgaat ttatcgccct atttttttgc
      361 aaataaatag tgaaaaaaga ccgcttgttt cagataaacc tagtcaaaag cttactaaat
      421 aggtacaatc actaacttgc attctaagct ttaaagtgcg attcgatttt attttggaga
      481 tatttatgtc gtggaatgaa tcaggcaatc agcaagatcc ttggggtaaa ccggggcaga
      541 aaaaagctga gccaaagcct gagcaacaag aacagggttc acaacaagag ccgaccaata
      601 atcaaagaaa tgaacaacag ccccctgatt tagaagaagt atttagcagt ttattaaaaa
      661 aaatgggggg tggaaaaggg cctaataata gttcaaatca acctagtgca agcttaggga
      721 aatttctacc tgtggtttta ggtcttgcag cagtagtttg ggttggctca ggtttttaca
      781 ctgtgcaaga ggctgaacgt ggtgtggtta ctcgtttagg taagctgaat gatatcgtgt
      841 tgccagggtt gaattggaaa ccgactttta ttgattcggt tacaagagta aacgtagaac
      901 gtgtttcaga gctgaacacc agtggctcaa tgctgactca agatgaaaat atggtgcagg
      961 tagaaatgac ggttcagtat cgtgtggaag acccggctaa atatctgttt agtgtgagca
     1021 accctgatga cagcttaaaa caagcaacag acagtgcctt gcgttatgtg attggtcata
     1081 tgacgatgga tgaaatttta accacagggc gtgcaaccgt gcgtgaaaga acgtggtcga
     1141 cattgcgtga tattatcaaa acctatgata tgggtttatt ggtaacagac gtaaacttcc
     1201 aatatgcacg accgcctgaa gaggtgaaag ccgcctttga tgatgcaatc aaagcacaag
     1261 aagatgagca acgcttaatt cgtgaagcgg aagcctatgc aagaggtgaa gagccgattg
     1321 cacgtggcca ggcacagcgt acgattgagc aagctcaagc ttacaaagaa gcagttgtgt
     1381 taaatgcgaa aggggaagtg gagcgtttga gtcaattatt acctgaatat aaagcctctc
     1441 cagagctgac tcgtgaacgt ttatatatcc agacaatgga aaaagtcatg aaaaatacgc
     1501 cgaaagtggt gatggattca agtggtaata atttgaatgt attgcctttc gataagctaa
     1561 tgaattcttc atcggtaata aaagcggagc agcctgcggc ggcacaagta gttacaccac
     1621 aagttgttca gccgcaagta cagccaagaa cacaaattca gccgcaacaa gcagaacaac
     1681 aagaaccaac tcgtaagggg agattttaat catgcgtaga ttattagtac ctatacttgc
     1741 tgtagttgct tttgttgtgc tacaggcaat caccattgta aacgagggcg agcgtggcat
     1801 tatgttacgt tttaacaaag ttcatcgtga tagcgatcaa aaagtggtgg tatatgaacc
     1861 ggggattcac tttaaagtac catttatcga tagtttaaag gtattagatg ctcgtattca
     1921 aactctagat gggcaagaag accgttttgt aacagtcgag aaaaaagatt tattagtgga
     1981 ctcctatgta aaatggcgaa tcagtgattt tggccaattt tatacctcta ctggggggga
     2041 ttaccaaaaa gcggcggatt tattacgccg taaggtaggg gatcgtctgc gttccgagat
     2101 tggttctcga accattaaag atattgtatc cggctctcgt ggtgagttaa tggcaggagc
     2161 ccaaaaagcg ctgaatgccg gtgaagatgg agcagagcgt ttaggaattg aagtggttga
     2221 tgtgcgagtg aagcaaatca acttaccaaa tgaagtttct tcttcaattt accaacgtat
     2281 gcgtgccgag cgtgatgcgg ttgctcgtga acaccgctca caaggtaatg agaaagcaga
     2341 ggtaattcgt gccgaagtgg ataaaaaagt ggtgctgatt ttggcaaatg cgaacaaaac
     2401 tgcccaagcc ttgcgaggtg aaggtgacgc tcaagctgca aaactctatt cagagaaatt
     2461 tggtaacgag ccggaatttt acagttttat tcgtagttta aaagcctatg aagatagctt
     2521 tgccgaaggt caaaataata tgatgttatt aaagccaaac agcgaattct tacgctttat
     2581 gcaagcaccg acaaaataat tgtatctata aaacccgatc ttgtatcggg tttttaattt
     2641 tcatccctat tctgatcata acaagcggta aattttggct gattttttgc aaaaaatttg
     2701 aaaaaattga ccgcttgcga tatgaagtta taatgggttt cgttattcat cctaactaaa
     2761 agaggcattt ctatgggtaa aagtgttgct attcttggcg ctcagtgggg cgatgaggga
     2821 aaaggcaaaa ttgttgattt attaacagat cgtgtgaaat atgtagtacg ttatcaaggt
     2881 ggacataatg cagggcatac cttaatcatt aatggtgaaa aaaccgttct gcgtttaatt
     2941 ccatcgggta ttttgcgtga taacgtgact tgtttaatcg gtaatggtgt agtgctttct
     3001 ccagaggcat taatgaaaga gatgggggag ctagaagaac gaggcattaa tgtgcgtgag
     3061 cgtttaaaaa tttctgaagc ttgcccgtta attctgcctt atcacgttgc gatggatcac
     3121 gctcgagaag cggcacttgg taaaaacaaa attggtacaa caggacgtgg tattggccca
     3181 gcttatgaag ataaagtcgc tcgccgtggt ttacgtgtca gcgatttatt cgacaaagaa
     3241 cattttgccg caaaattaaa agatatttta gattactaca acttccaatt agtcaattat
     3301 tacaaagttg aagcggtaga ttatcaaaaa accttagatg atgtgtttgc gattgcggat
     3361 gtgattaccg gaatggttgc cgatgtgaca actttacttc acgaagcacg tgaaaacggc
     3421 gacaatattt tatttgaagg ggctcaaggc acgatgttag atatcgacca cggtacttac
     3481 ccgttcgtaa ccagctcgaa tacgacagca ggcggtgtgg caaccggatc aggctacggt
     3541 cctcgtcatt tagattatgt attagggatt atcaaagcct actgtacccg tgtaggtagt
     3601 ggaccgttca ccaccgaact atttgacgat gtaggtgctg aaattgctcg caaaggtaat
     3661 gagttcggag cggtaaccgg tcgccctcgc cgttgtggct ggtttgatgc cgtagccgtt
     3721 cgccgagcag tgcaaatcaa ctccatttca ggcttctgta tgactaaact cgatgtttta
     3781 gacggattca aagagctaaa aatctgcgtt gcctacaaaa tgccaaacgg aaaaattgtc
     3841 gaatatgccc ctcttgcagc aaaagactgg gaaggtgttg aaccaattta tgaaacaatg
     3901 ccgggttggt ctgaaaatac tttccgtgtg acaaaacgtg aagatttacc acaagccgca
     3961 ttagattaca ttaaacgtat cgaagagctg gtcggtgtgc cggtagatat tctttctaca
     4021 ggtccggatc gtgtcgaaac gatgattttg cgtgatcctt ttgcagctta ataaaagata
     4081 aaataaagcc atttagtgag atagagctag atggcttttt tgtttgaacc agtaaacttt
     4141 actttggcac aattttgtca caatacagaa aatagttctt tagcgtgagt ttttgaggct
     4201 gtgtaagtat ttgaatttaa aagaatttaa tggcacgccc taaaggattc gaacctttga
     4261 cccacgcctt agaagggcga taggtatttt ataaaaggcg tttattttaa ctaataaaaa
     4321 caaagactta tagagataac ccctctatat tacctaccat tatttcacat ctttttacat
     4381 taaataacac catttacggt acatttatgg aacacgttgg tgatgagcta gtcttcaaaa
     4441 gttactaaat taaactacaa tctgccccgt ctcttttaag tacttataga tacgccctaa
     4501 cataatctgc gttggcgttt tgccaatatc ctcttttgtt agcggtgcat cagtaatc
//
LOCUS       DANLZC010000162         4482 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.162, whole genome shotgun sequence.
ACCESSION   DANLZC010000162 DANLZC010000000
VERSION     DANLZC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.162"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            201..1520
                     /locus_tag="PXE44_002280"
     CDS             201..1520
                     /locus_tag="PXE44_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL5589745.1"
                     /translation="MNHLLYSVEDKPPFGLSLLLASQHLLAALGGIIAVPLVLGNVLK
                     LSTADTIILVNAALLISGIVTIIQCRGIGPIGIRLPSVMGTSFTFVAAALAIGFSESG
                     IAGIMGASLVGSLVMIIGSFFMPYIRKLFPPVVTGTVIMMIGLSLIPVAVDWFAGGQR
                     TDPHYATPENLIMASFVLIIVVALVQWGKGIFSAAAIVIGMMIGYIVSLALGWVSFNG
                     VKEAQLFALPQPLHFGLEFPISGIIGMSIAYLVTIVESSGNFLALGNATKTEITGKHL
                     RGGVLCDGLGSAFAAIMSTTPFSSFSQNIGVISLTGVASRYVVAFTGALLVLAGLFPV
                     FGALIVSIPLPVLGGAGLMMFAMIIAAGIQMLDNIQRSKRNGLIIAISIGCGLAVTTR
                     PELLDKLPHFFKEVFGSGITVGSLLALILNLVLPEDKITKKENKSDE"
     gene            complement(1690..3633)
                     /gene="ftsH"
                     /locus_tag="PXE44_002281"
     CDS             complement(1690..3633)
                     /gene="ftsH"
                     /locus_tag="PXE44_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL5589746.1"
                     /translation="MVKNILLWVVIAIVMMTAYEGFNSNFSGSSNTVPYSTFLSDIKE
                     NRLKGVDFKQNDDTILVTKNDGATYQTVMPMYDEYLMADLAKTNAVITGQPAERRGLL
                     SQILISWFPMLLLIGFYVFYMRQMQGGGGRGAMGFGKNKAKMLTADEVKTRFSDVAGC
                     DEAKEEVGEVVEFLRDPTKFQKLGGRIPKGILMVGPPGTGKTLLAKAIAGEARVPFFT
                     MAGSDFVEMFVGVGASRVRDLFDQAKKNAPCIIFIDEIDAVGRKRGGAGFSGGHDERE
                     QTLNQMLVEMDGFEGSEGVIIIAATNRADVLDDALTRPGRFDRQVTVDLPNVKGREQI
                     LKVHLKKVPLAEGVDPMQIARGTPGYSGAQLANLVNEAALFAARKNKRVVMMEDFEEA
                     RDKINMGPERRSNTMTEKEIINTAYHEAGHVIVGYLMPEHDPLNKVTIVPRGQALGFA
                     QFLPEGDRVSETFTKLESQLSTLFAGRIAEGLIFGEDKITTGASSDIHRATQIARAMV
                     TQWGFSKELGPIFYQNEDGMGAIKGVSEESQKLIDVEMRKIIDRNYQRAKQTLEENMD
                     ILHAMKDALLKYETLDSKQIDDLMARRPVGEPSGWTDTPTKDDNATPPSGEQAADVSS
                     TLEDNPNKDNNIGDSLADTKAEH"
     gene            complement(3754..4380)
                     /gene="rlmE"
                     /locus_tag="PXE44_002282"
     CDS             complement(3754..4380)
                     /gene="rlmE"
                     /locus_tag="PXE44_002282"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDL5589747.1"
                     /translation="MGRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQT
                     DRLFKPGMTVVDLGAAPGGWSQYVVTQIGSKGRVIACDILDMNPIVGVDFLQGDFREE
                     SVLNALLERVGEDKVNVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLATKGSF
                     VVKVFQGEGFDEYLREIRSLFSVVKVRKPEASRDRSREVYIVATGYKG"
BASE COUNT         1223 a         1018 c          829 g         1412 t
ORIGIN      
        1 ttttttgtta caactggaaa gaaattggta caaacattcg gattagcttt gcaaaaagcg
       61 taaaaaatct taccgcttgt catttatcag ttagggaaaa taaaaaatca ctctcttata
      121 taaaaacaat tacgcaaacg attgcttatt aaaaaaactt cagtataatg ccgacatttt
      181 ttaactctcg aggaatcact atgaaccatt tactttattc tgtcgaagac aaaccgcctt
      241 tcgggctgag cttattgctt gcctcacaac atttattagc cgctttaggt ggcattatcg
      301 ctgtaccttt agtgctaggg aatgtactca aactctctac tgcggatact ataatcttag
      361 ttaatgctgc acttttaatt tcaggcatcg tcactattat tcaatgtcga ggtattggcc
      421 caatcgggat tagactaccg agtgtaatgg gaactagctt tacgtttgtt gcagcagcat
      481 tagcgattgg tttcagtgaa agtggcattg ccggcattat gggagcctct ttagttggct
      541 ctctagtgat gattatcggc agttttttta tgccttatat ccgtaagctt tttccacccg
      601 tggtaacagg tactgtcata atgatgattg gcctaagttt aattcctgtt gcagtagact
      661 ggtttgctgg tggtcagcgt actgatccac actatgcaac cccagaaaat cttataatgg
      721 ctagttttgt attaattatt gtcgttgctt tagtacaatg ggggaaaggt attttttcag
      781 ctgctgctat tgtgattggt atgatgatag gttatatcgt ttcgctagct ctgggttggg
      841 taagttttaa tggcgtgaaa gaagcacaac tttttgcgtt accacaacct ctacattttg
      901 gcttagaatt tccaatctcc ggtatcatcg gaatgtcaat tgcttattta gttaccattg
      961 tagaatctag cggaaatttc ttagcactag gcaatgcaac taaaactgaa attaccggaa
     1021 aacacttacg tggaggtgta ttatgtgatg gtttaggctc agcatttgcg gcaattatgt
     1081 ccacaactcc cttttcttct ttctcacaaa atattggggt catttcatta accggtgtag
     1141 ccagtcgcta tgttgtagca tttacaggcg cattacttgt actagccggt ttatttcctg
     1201 tatttggagc attaatcgtg tctatcccgt tacctgtgct tggcggtgca ggcttaatga
     1261 tgtttgccat gattatcgca gccggtatcc aaatgttaga taatattcaa cgtagcaaac
     1321 gaaacggttt aattattgct atctctatcg gttgtgggct ggccgtaaca actcgacctg
     1381 agctattgga taagcttcca cacttcttta aagaagtttt tggctcaggt attacggtcg
     1441 gctccttatt agcacttatt ttgaatttag ttttacctga agataaaata accaagaaag
     1501 aaaataagag tgatgaataa atcaccctaa aaaaacaagg acagctcaca atacaacttt
     1561 gatgctgtcc tttattccca tagagagcat atttaaattt aggttaataa aaaaatctgc
     1621 actctatatt ggtgtttacc tttagagtac agatttcttc ttattctaac tttctagcaa
     1681 acaattagat taatgttccg ctttggtatc ggctaacgaa tccccaatgt tattatcttt
     1741 atttggatta tcctctaaag ttgaacttac atccgctgcc tgttcaccac ttggtggagt
     1801 ggcgttatcg tctttagttg gggtatctgt ccaacctgat ggttcaccca ccggacggcg
     1861 tgccattaaa tcatcaattt gcttgctgtc taaggtttcg tatttcaata aggcatcttt
     1921 cattgcgtgt aagatgtcca tattttcttc caaagtttgc ttcgctcgtt gatagttgcg
     1981 gtcaatgatc ttacgcattt ccacatcaat caatttttgc gactcttccg atacgccctt
     2041 aattgcaccc attccatctt cattttgata gaaaatcggg cctaattctt ttgagaagcc
     2101 ccattgagta accattgctc gtgcaatttg ggttgctcga tgaatatcag aggaagcccc
     2161 tgtggtaatt ttatcttcac caaaaatcaa gccctcagca attcgccctg caaatagggt
     2221 ggaaagttgg ctttccaatt tggtaaatgt ttcgcttaca cggtcacctt ccggtaagaa
     2281 ttgagcaaaa cctaacgcct gaccacgagg cacaattgtc actttattca gcggatcgtg
     2341 ttccggcatt aaatagccaa caataacgtg tccagcttcg tggtaagcag tattgatgat
     2401 ctctttttcc gtcattgtat ttgaacggcg ttccggcccc atattgattt tgtcacgggc
     2461 ttcttcgaaa tcttccatca ttaccacacg tttattctta cgggcagcaa ataaggcagc
     2521 ttcgttcacc aagtttgcta attgagcacc agagtaaccc ggcgtaccac gagcaatttg
     2581 catcgggtca actccctctg caagcggtac ttttttcaag tgaactttca aaatttgctc
     2641 acgccctttc acgttcggca aatcgaccgt gacttggcgg tcgaaacgcc ccggacgagt
     2701 taaggcatca tctaacacat cggcacggtt ggttgcagcg atgataatca caccttcaga
     2761 gccttcaaag ccgtccatct caactaacat ttggttgagg gtttgttcac gctcatcgtg
     2821 tccaccgctg aaacctgcac caccacgttt acgccctacc gcatcaattt catcaataaa
     2881 aataatacaa ggggcatttt tcttggcttg gtcgaataaa tcacgcaccc gtgaagcacc
     2941 cacacctaca aacatttcca cgaagtcgga acctgccatt gtaaagaatg gcactcgtgc
     3001 ttcacctgca atcgctttcg ccaataaggt tttacccgta cccggagggc ctaccatcaa
     3061 gatccctttt ggaatgcgcc cacctaattt ttgaaattta gttgggtcac gcaaaaactc
     3121 aacaacttcg cctacttctt ctttggcttc atcacagccg gctacatcag aaaatctggt
     3181 cttaacttcg tctgcggtca acattttggc tttatttttc ccaaagccca ttgcaccacg
     3241 tcctccgcca ccttgcattt gtcgcatata aaacacatag aaaccgatta acagcaacat
     3301 tgggaaccaa gaaatcaaaa tttgcgagag caagccacga cgctcagcag gctgaccggt
     3361 aatcactgca ttggtttttg ctaaatctgc cattaagtac tcatcataca tcggcattac
     3421 ggtttggtag gttgcaccat catttttcgt caccaaaatg gtgtcatcat tctgtttgaa
     3481 atcgacccct tttaaacgat tttctttaat atcgcttaaa aaagtcgaat atggcactgt
     3541 atttgagcta ccactgaaat tagaattaaa accttcatag gctgtcatca tcacaatggc
     3601 aatcaccacc caaagtaaaa tatttttaac catatcgttc aaggttaatc ccctatctgc
     3661 tgaataaaaa actgttcaag attactataa aagcggtcga attttccaat ttttttgcaa
     3721 aatgttaata aaaaatgacc gcttgcttgt tttctagcct ttataacctg tcgctacaat
     3781 ataaacttct cgagaccgat ctcgagacgc ctccggtttt ctgactttta ccacactaaa
     3841 gagcgagcgg atttctcgaa ggtattcatc aaatccttcg ccttggaata ccttaaccac
     3901 aaaactgcct ttggttgcta atacttggcg acacatatcc aatgccaatt ccactaaata
     3961 cattgcacga ggaatatcaa ccgacggcat accgctgaaa ttgggagcca tatccgacat
     4021 caccacattc actttgtctt cgcctactcg ctcaagtaac gcattcagca ccgattcttc
     4081 acggaagtcg ccttgtaaga aatccacgcc tacaatcggg ttcatatcta aaatatcaca
     4141 agcgattact ctgcccttac tgccgatttg cgtcaccaca tattgcgacc aaccgcccgg
     4201 tgcggcaccg agatccacca ccgtcattcc cggtttgaat aaacggtcgg tttgttgaat
     4261 ttcatctaac ttaaaatacg cccgtgaacg caatttttgt ttatgtgctt tttgcacaaa
     4321 ttgatcttta aaatgttctg caagccaacg tgatgaactc gcacttcgtt ttctacccat
     4381 ttgttcttct atcttaatta attcaacgat ttataataag ggttaaatta gcaaattcaa
     4441 gagatgttct aaaatttagc tcggcttatt atgcttaatt aa
//
LOCUS       DANLZC010000163         4404 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.163, whole genome shotgun sequence.
ACCESSION   DANLZC010000163 DANLZC010000000
VERSION     DANLZC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.163"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            51..1157
                     /gene="recA"
                     /locus_tag="PXE44_002283"
     CDS             51..1157
                     /gene="recA"
                     /locus_tag="PXE44_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL5589748.1"
                     /translation="MAEKKSQKNTPVKQIDPEQKEKALAAALAQIEKQFGKGSIMKLG
                     DTQALDIEAVSTGSLGLDSALGIGGLPMGRIVEIYGPESSGKTTLTLSVVAQAQKNGK
                     TCAFIDAEHALDPIYARKLGVDTDGLLISQPDNGEQALEICDALVRSGAVDVIIVDSV
                     AALTPKAEIEGDMGDSHMGLQARLMSQALRKLTANIKATNCLVIFINQIRMKIGVMFG
                     NPETTTGGNALKFYASVRLDIRRSGVVKDGDEVIGSETKVKIVKNKVAPPFREVQFDI
                     MYGEGIARMNELLILAESHGFINKAGAWFSYEGEKIGQGKNNAIKWLKEHPEVASKIE
                     QDIRNLLISNPTFTATPDSENADNADDEFSEEEL"
     gene            1216..1692
                     /gene="recX"
                     /locus_tag="PXE44_002284"
     CDS             1216..1692
                     /gene="recX"
                     /locus_tag="PXE44_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL5589749.1"
                     /translation="MTTSTTKTNKYTAVNYLLYLLSKRDYSEQELRQKLKQKEYELTE
                     IDAAIEKAQANQWQSDERFCTTFIRYRSMQGIGPRRLKQELKLKGVKDWLINQELENA
                     EVDWFELAEQIFEKKRPLVWDLKAKQKMWRFMVSRGFYNDHFSHLMALDYSSDEYE"
     gene            1685..1996
                     /locus_tag="PXE44_002285"
     CDS             1685..1996
                     /locus_tag="PXE44_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL5589750.1"
                     /translation="MNREKITFFSRFEADILSGKKTITIRDKSESYFQPNQELAVFTN
                     ETDRFFANIKVLSVTPIVFDELNEQHAKQENMSLAELKQVIREIYPNDNAFFVIEFTL
                     I"
     gene            2006..2542
                     /locus_tag="PXE44_002286"
     CDS             2006..2542
                     /locus_tag="PXE44_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDL5589751.1"
                     /translation="MVLFKSTKQTETCPQCGSPLQIKQGKKGLFLGCSAYPECDYIKP
                     LHQAGHIIKQLDELCPECGGNLQLKQGNYGIFIGCSHYPECHFIVHEEAEVEEEFDCP
                     ECKQHKLIARKGRSGKIFYGCIGFPNCKFTLATRPINHLCEKCGCELATIKKIKGKQF
                     TICANKSCQHQTELKPNE"
     gene            2535..3089
                     /locus_tag="PXE44_002287"
     CDS             2535..3089
                     /locus_tag="PXE44_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDL5589752.1"
                     /translation="MNNFLEIVNQLKQEEVVAYPTEAVFGLGCNPQSEKAVRKLLVLK
                     QRPEEKGLILLASDLKYLLPYIDEARMTEQAWQRLSHIGEQAITWIVPVKNTVPSYLT
                     GKFDSIAVRLCNVPAVVQLCEASGVALTSTSANLSGEPPCRNAKEVIDQFGQDFPVLH
                     ADTLGRENPSEIRDIFSQQIFRQG"
     gene            3096..3920
                     /gene="aroE"
                     /locus_tag="PXE44_002288"
     CDS             3096..3920
                     /gene="aroE"
                     /locus_tag="PXE44_002288"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL5589753.1"
                     /translation="MNRYAVWGNPIAQSKSPRIHQLFALQTQRNIEYIANLGDETAFE
                     QQLADFFASGAKGCNITAPFKERAFKLADEFSETALLAQACNTLKRLEDGRLYADNTD
                     GAGLVSDLERLGWLQKGHKTLILGAGGATKGVLLPLLKAGLEITLYNRTQEKAVVLAE
                     KFAKFGKIQTACLSDLANQLFNLIVNATSLGLQGKYVELPESVIASANIYDMQYAPNM
                     QTPFLNYARRCGATACQDGLGMLVGQAAFAFSLWEGQFPDVEPVLAQLKLEMQTVK"
     gene            4087..4163
                     /locus_tag="PXE44_002289"
     tRNA            4087..4163
                     /locus_tag="PXE44_002289"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4121..4123,aa:Met,seq:cat)
     gene            4166..4250
                     /locus_tag="PXE44_002290"
     tRNA            4166..4250
                     /locus_tag="PXE44_002290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4200..4202,aa:Leu,seq:tag)
     gene            4276..4350
                     /locus_tag="PXE44_002291"
     tRNA            4276..4350
                     /locus_tag="PXE44_002291"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4308..4310,aa:Gln,seq:ttg)
BASE COUNT         1420 a          822 c          949 g         1213 t
ORIGIN      
        1 ttgcagcaac aagcggttgc ttttagataa aagtttacaa aggatagaat atggcagaga
       61 aaaaaagtca aaagaacact ccggttaaac agattgatcc ggaacaaaaa gaaaaagcgt
      121 tggcagccgc attagctcaa attgaaaagc aatttggcaa aggctcaatt atgaagttag
      181 gcgatactca agcccttgat attgaagcag tttcaaccgg ttctcttggt ttagactcag
      241 cattaggcat tggtggttta ccaatgggtc gtattgtgga aatttatgga ccggagtctt
      301 caggtaaaac tacattaact ttatctgtgg tggctcaagc ccaaaaaaat ggtaaaactt
      361 gtgcctttat tgatgccgaa cacgcacttg atcctattta cgcccgtaag ttaggggtag
      421 atacagatgg attattaatt tcacaaccgg ataatggcga acaagcatta gaaatttgtg
      481 atgcgttagt tcgttccggt gcggttgatg taattattgt ggactctgtt gcggcactta
      541 caccaaaagc agagattgaa ggcgatatgg gtgattccca tatgggctta caagctcgtt
      601 taatgtcaca agcattgcgt aaattaaccg caaatatcaa agcgaccaac tgcttggtga
      661 ttttcattaa ccaaattcgt atgaaaattg gagtaatgtt cggtaaccct gaaaccacaa
      721 ctggtggtaa tgcccttaaa ttctatgctt cagtacgttt agatattcgt cgttcaggcg
      781 tagtaaaaga cggtgatgag gttatcggta gcgaaacaaa agtgaaaatt gtgaaaaaca
      841 aggttgctcc accgttccgt gaagtccaat ttgatattat gtacggtgag ggcattgccc
      901 gtatgaatga attactcatt ttagcggaat ctcacggctt cattaataaa gcaggggctt
      961 ggttctctta tgaaggggag aaaatcggtc agggtaaaaa taatgccatc aaatggttaa
     1021 aagaacaccc tgaagtggcg agtaaaattg agcaagatat tcgaaatcta ctcatttcaa
     1081 acccaacttt tacggctact ccagattctg aaaatgcaga caatgcagat gatgaattta
     1141 gtgaagaaga actctaatta aatttatgcg gttttcgctc aacgtgaaaa tcgcattttt
     1201 atttataacc gataaatgac aacatctact actaaaacaa ataaatacac agcggttaat
     1261 tatctgctat atttactttc aaagcgtgat tacagcgagc aagagcttcg gcaaaaacta
     1321 aagcaaaaag agtatgaact cacggagatt gatgcagcga ttgaaaaagc tcaagcgaat
     1381 cagtggcaaa gtgatgaacg cttttgtacc acttttatcc gttatcgttc aatgcaaggc
     1441 attgggcctc gccgtttaaa gcaagagcta aaacttaaag gggtaaaaga ttggttaatt
     1501 aaccaagaat tagaaaacgc cgaagtggat tggtttgaat tagctgagca aatttttgag
     1561 aaaaaacgac cgcttgtatg ggatctgaaa gcaaagcaga aaatgtggcg atttatggtt
     1621 agccgtggtt tttataacga tcattttagt catttaatgg ctctggatta cagtagcgat
     1681 gaatatgaat agagagaaaa ttaccttttt cagccgattt gaagccgata ttttaagcgg
     1741 taaaaaaaca attacgattc gagacaagtc agaatcttat tttcaaccaa accaagaatt
     1801 agcggttttt actaatgaaa ccgatagatt tttcgccaat attaaagtgc tatcggtaac
     1861 acctatagtg tttgatgaat taaacgagca acacgctaaa caagaaaata tgagcttagc
     1921 cgaattgaaa caagttattc gggagattta tccgaatgac aatgcgtttt ttgtgattga
     1981 atttacgctt atttagcaaa tcaatatggt acttttcaaa tcaaccaaac agaccgaaac
     2041 ttgcccacaa tgtggctcgc cgttgcaaat taagcagggc aaaaaagggt tatttttggg
     2101 ttgttcagct taccctgaat gtgattatat taagccatta catcaagccg gtcatattat
     2161 taagcaatta gatgaacttt gccctgaatg tggcggaaac ctccaattaa aacaaggcaa
     2221 ttatggtatt tttatcggct gtagccatta ccctgaatgc cacttcatcg tacacgaaga
     2281 agctgaggtt gaagaagagt ttgattgccc tgaatgcaag caacataaac tgattgctag
     2341 aaaagggcgt tcaggcaaaa tattttatgg ttgtataggc ttccctaatt gcaaatttac
     2401 attagcaact cgaccgatta accacttgtg tgaaaaatgt ggttgtgaac tcgctacaat
     2461 caaaaaaatt aagggcaagc aattcaccat ttgtgctaac aaaagttgcc agcaccaaac
     2521 agaattaaaa ccgaatgaat aattttttag aaatagttaa tcaattaaag caagaagaag
     2581 ttgtagctta cccaaccgaa gcggtttttg gcttaggctg caatccacaa agtgaaaaag
     2641 cggtaaggaa attactcgta ttaaaacaac gccctgagga aaaagggcta attttactgg
     2701 cttctgattt aaagtatctt cttccctata ttgatgaagc tcgaatgacg gaacaggctt
     2761 ggcaacgcct aagccatatt ggcgagcagg caattacttg gatcgtaccg gttaaaaata
     2821 ctgtgccaag ttacctcacc ggcaaatttg attctattgc cgtacgttta tgtaatgtgc
     2881 cggcggtggt tcagttgtgt gaggcaagcg gcgttgcgtt gacctctacc agtgccaatt
     2941 tgagcggaga accaccttgc cgcaatgcaa aagaagtcat cgatcagttt ggtcaagatt
     3001 ttcctgtatt acacgcagac acgctaggta gagaaaaccc ttccgaaatt cgagatattt
     3061 tcagccaaca gatttttaga caaggataag ccgaaatgaa ccgatatgcc gtgtggggca
     3121 acccgattgc tcaaagtaaa tccccccgta ttcatcaact ctttgcttta caaacccaac
     3181 gcaacattga atatatcgct aacttaggag atgaaacggc ttttgagcaa caattagccg
     3241 attttttcgc ttctggagca aaaggttgca acatcaccgc cccttttaaa gaaagagcct
     3301 ttaaattggc agatgaattt agcgaaaccg ctttgttggc acaggcgtgt aatacgctta
     3361 aacgcttaga agatggacgg ttatatgccg ataataccga cggggccggt ttagtttctg
     3421 atttggaacg gctaggttgg ttacaaaaag ggcataaaac cttaattctt ggagcaggtg
     3481 gagcaaccaa aggcgtatta cttccgttac ttaaagcggg gttagagatt actctatata
     3541 accgcacgca ggagaaagcg gtcgttttag ccgaaaaatt tgcaaaattt ggcaaaattc
     3601 agaccgcttg tttaagcgat ttggcaaacc aactgtttaa tttaatcgtc aatgccacct
     3661 ctctcggtct gcaaggcaaa tatgttgaat tgcctgaatc tgttatagca tcggcgaata
     3721 tttacgatat gcaatatgcc cccaatatgc aaactccttt tctcaattat gcaaggcgtt
     3781 gtggagcaac agcgtgccaa gacggattag gaatgttggt tggacaggca gcgtttgcgt
     3841 tttctttgtg ggaagggcaa ttccccgatg ttgagcctgt actggctcaa ttaaaattag
     3901 aaatgcaaac ggtgaaatag cagaatataa gctattattt tataaacaaa aaatgctaga
     3961 tttaagccaa aacgacgaat aactatacaa gcgaatcaaa aattcaaatt ttttaaaaaa
     4021 agtatttgac ggaaagccct aaaatcagca taatgcactc cgttgaccga tatggtgaac
     4081 gcaaatggct acatagctca gctggttaga gcacaacact cataatgttg gggtcgcaag
     4141 ttcgaatctc gctgtagcca ccattgcggg actggcgaaa ttggtagacg caccagattt
     4201 aggttctggc gccgcgaggt gtgtgggttc aagtccctcg tcccgcacca tttatcgatt
     4261 aacaaaaatt agtgttgggg tatcgccaag cggtaaggca ccgggttttg atctcggcat
     4321 ccctaggttc gaatcctagt accccagcca cttaatttca aacattcaaa tctatataag
     4381 ctaacgtaag ttagtttttt cttt
//
LOCUS       DANLZC010000164         4328 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.164, whole genome shotgun sequence.
ACCESSION   DANLZC010000164 DANLZC010000000
VERSION     DANLZC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.164"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            115..1323
                     /locus_tag="PXE44_002292"
     CDS             115..1323
                     /locus_tag="PXE44_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL5589754.1"
                     /translation="MSKNLILILNCGSSSLKFAILDPKTGDEKLSGLAEAFHLEDARI
                     KWKLNGEKGNADLGAGAAHSEALTFIAEKLLSDELKAGIGAIGHRIVHGGEKFTSSVV
                     ITDEVIKGIEDATQFAPLHNPAHLIGIKEAFRIFPELKDKNVAVFDTAFHQTMPEHAF
                     LYALPYTFYKENGIRRYGFHGTSHFYITSQVAELAGKSVEQTNAIICHLGNGGSVSVV
                     RNGKCIDTSMGLTPLEGLVMGTRSGDLDPAIISFLYNNLGMSMDEIDTTLVKKSGLLG
                     LTGVTSDCRYAEDNYNDVSKPEAKRALDVYSYRLAKYIGSYMAILGDDHLDAIAFTGG
                     IGENSGLVRELTLNHLKLFGIKLDQERNLAARFGKDGVITADESTFKAVVIPTNEELV
                     IAQDTAKLAL"
     gene            1376..3511
                     /gene="pta"
                     /locus_tag="PXE44_002293"
     CDS             1376..3511
                     /gene="pta"
                     /locus_tag="PXE44_002293"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005542370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL5589755.1"
                     /translation="MSRTIILIPISTGVGLTSVSLGLVHALEQKGSKVGFLKPISQPI
                     SGEDTLDRSTTIIRAEQTTEVGEPFMLSEAETLIGQNQTDVLLEKVVERHQKLSKNNE
                     IVIVEGLIPTRKNSYANSINYEMAQALDAEIVLVAAPASDTPAQLKERVEAAASQFGG
                     RNNPNLLGVIINKFNAPVDDSGRTRPDLTEIFDSFQHSNNNVSEVEHLFAKSPIKLLG
                     CISWKAELIATRAIDVAKHLGASIINEGEIKARRIRGVTFCARNLPNMVDHFKAGSLL
                     VTSADRPEVLVAASLAVMNGIEIGAILLTGGYKIDSSIAKLCQQAFETGVPVFRIEGN
                     TWQTALSLQSFSLEVPVDDKERIAAIKEYMASKFNVGFIDEVSKAATRARRLSPAAFR
                     YQLTEYARQAKKRIVLPEGDEPRTVKAAALCAERGIAECVLLANPTDVQRVAEAQGVV
                     LGKGVTIIDPASIRENYVDRLVELRKAKGMTEVVAREQLLDTVVLGTMMLEANEVDGL
                     VSGAVHTTANTIRPPMQIIKTAPGSSIVSSIFFMLLPDQVLVYGDCAVNPDPTAEQLA
                     EIAIQSAESAKAFGIDPRVAMISYSTGTSGSGADVEKVKEATRIAQEKRPDLIIDGPL
                     QYDAAIMEDVARSKAPNSPVAGKATVFVFPDLNTGNTTYKAVQRSADLVSIGPMLQGM
                     RKPVNDLSRGALVDDIVYTIALTAIQATQ"
     gene            3625..4305
                     /gene="can"
                     /locus_tag="PXE44_002294"
     CDS             3625..4305
                     /gene="can"
                     /locus_tag="PXE44_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDL5589756.1"
                     /translation="MEKIERLFANNHAWATKMKDEQSDYFKELAEHQKPTYLWIGCSD
                     SRVPAEKLTGLGPGELFVHRNVANMVIHTDLNCLSVVQYAVDVLEIEHIIICGHTNCG
                     GINAAIAQKELGLINNWLLHIRDIWFKHSHLLGKLPYEERSNVLTRLNVAEQVYNLGQ
                     SSIIKTAWEQGKSLSLHGWVYDVTDGFLIDQGVMATSRETLEITYRNAIAKITTEANE
                     KIVLNDEN"
BASE COUNT         1357 a          801 c          878 g         1292 t
ORIGIN      
        1 tttgagttca agcatttatt ttaagatctg aaaggtgtat cctttacacc ttaaatacac
       61 caataaaaat agtattttct tcaaactcat ttcataaaat aaggtaattt gaccatgtct
      121 aaaaacttaa tcttaatcct taactgcggt agttcatctc ttaaatttgc tattttagat
      181 cctaaaacag gtgatgaaaa attatctgga cttgctgaag cttttcattt agaagatgca
      241 cgtatcaagt ggaagttaaa cggggagaaa ggtaatgctg atttaggtgc aggtgccgca
      301 catagtgaag cacttacctt tattgctgaa aaattgttat ctgacgaatt aaaagcaggt
      361 atcggcgcga ttggtcatcg tattgttcat ggcggtgaaa aattcacctc atcagttgta
      421 attaccgatg aagtgattaa aggtattgaa gatgcaacgc aatttgcacc attacacaac
      481 cctgcgcatt taattggtat taaagaagca ttccgcattt tccctgaatt aaaagacaaa
      541 aacgtagctg tatttgacac tgcattccat cagacaatgc cggaacacgc attcttatat
      601 gcacttccat atacattcta taaagaaaat ggtattcgtc gctacggttt ccatggcaca
      661 agccacttct atatcacctc acaagttgca gaattagcag gtaagtctgt tgagcaaacc
      721 aatgccatta tttgccactt aggtaacggt ggttctgttt ctgttgtacg caatggtaaa
      781 tgtattgata cctcaatggg tttaactcca ttagaaggtt tagtaatggg tactcgctct
      841 ggtgacttag accctgcgat tatctctttc ttatacaata acttaggtat gtcaatggac
      901 gaaattgata ctactctagt aaaaaaatct ggtcttttag gtttaactgg cgtaactagt
      961 gactgtcgtt atgcggaaga taattacaat gacgtatcaa aaccggaagc aaaacgtgct
     1021 ttagatgtat atagctatcg tttagctaaa tatattggtt catatatggc aattttaggc
     1081 gatgaccact tagatgctat cgcatttaca ggtggtatcg gtgaaaattc aggcttagta
     1141 cgtgaattaa ccttaaatca cttaaaatta tttggcatta agttagatca agagcgtaac
     1201 cttgctgccc gttttggtaa agatggagtc attacagcgg acgagtcaac atttaaagct
     1261 gttgttatcc caactaatga agaactcgtg attgcacaag ataccgctaa attagcatta
     1321 taatcatagg gggcagatat tctgcccttt cactttcttt taatttggaa atattatgtc
     1381 tagaacgatc attcttatcc ctatttcaac tggtgttggt ttaaccagtg ttagcttagg
     1441 tttagtacat gctttggagc aaaaaggttc taaagtagga tttttaaaac cgatttctca
     1501 acctatcagc ggtgaagata cgttagaccg ctcaacaacg attatcagag cagaacaaac
     1561 aacagaggtt ggtgagccgt ttatgttaag cgaagctgaa acattaatcg gtcaaaacca
     1621 aacggatgta ttgttagaaa aagtggtgga acgccaccaa aaattatcaa aaaataatga
     1681 aattgtcatt gttgaaggtt taattccaac gcgtaaaaac tcatacgcta acagcattaa
     1741 ctatgaaatg gctcaagccc ttgatgctga aattgtatta gttgcagcac cggcctcaga
     1801 tacaccagct caattaaaag agcgagttga agctgccgct tcacaatttg gtggacgtaa
     1861 caacccaaat ttactgggcg ttattatcaa taaattcaat gcaccagtag atgattcagg
     1921 tagaacccgt ccagatttga ctgaaatttt tgattcattt caacactcaa ataataatgt
     1981 ttcagaagtc gagcatcttt ttgctaaaag tccaattaag ctgttaggct gtattagctg
     2041 gaaagcagaa cttattgcta cacgagcaat tgatgttgca aaacatttag gcgcttcaat
     2101 tattaatgaa ggcgaaataa aggctcgtcg gattcgcggt gtgacattct gtgcacgtaa
     2161 cttaccaaat atggtcgatc attttaaagc cggtagttta ttagttacct ctgcggatcg
     2221 tcctgaagtg ttagttgctg catcgttggc ggtaatgaat ggcatcgaaa ttggtgctat
     2281 tttactaacc ggtggttata aaattgatag ctcaattgca aaactttgcc aacaagcatt
     2341 tgaaaccggt gtgcctgtat tccgtattga aggtaacact tggcaaaccg cattaagcct
     2401 acaaagcttc agcttagaag tgcctgtaga tgataaagag cgtattgctg caattaaaga
     2461 atatatggca agcaaattta atgtaggttt cattgatgag gtttctaaag ccgctactcg
     2521 tgcaagacgt ttatcacctg cagcattccg ttaccaatta accgaatatg cccgtcaagc
     2581 gaaaaaacgc attgttttac ctgaaggaga tgagcctcgt actgtgaaag cggctgcatt
     2641 atgtgctgag cgtggcattg cagaatgtgt gttattagct aacccaacag acgtacaacg
     2701 tgttgctgaa gcacaaggcg tggtattagg taaaggcgta acgattattg accctgcgag
     2761 cattcgtgaa aattatgttg atcgcttagt tgaattgcgt aaagcaaaag gaatgactga
     2821 agttgttgct cgtgagcaac ttttagatac agttgtattg ggtacaatga tgcttgaagc
     2881 taatgaagtg gacggtttag tctcaggcgc agttcacaca accgctaaca ctattcgccc
     2941 accaatgcag atcattaaaa ctgcaccggg aagctctatt gtatcttcta tcttctttat
     3001 gctactacct gatcaagtat tggtatatgg tgactgtgcg gtaaaccctg acccaactgc
     3061 agaacagcta gcggaaattg cgattcaatc agcagaatct gccaaagcct ttggtattga
     3121 ccctcgtgtt gcaatgatct cctactctac cggtacatca ggttcaggtg cggatgtaga
     3181 aaaagtgaaa gaagcaactc gtattgccca agaaaaacgt ccagatttaa tcattgatgg
     3241 cccattacaa tatgatgcgg cgattatgga agatgttgcc cgctcaaaag caccaaattc
     3301 acctgtagca ggtaaagcaa ctgtatttgt cttcccggat ttaaatactg gtaataccac
     3361 ctataaagcg gtacaacgtt cggcagattt agtctctatc ggcccaatgt tacaaggtat
     3421 gcgtaaacct gtaaatgact tgtcccgtgg ggctttagtt gacgacatcg tttatactat
     3481 tgcgttaact gcgattcaag caacgcaata attccctccc aaatgaaacc aaatgccctt
     3541 aaaactaagg gcatttttat tttttaccta cccgactttt caatttaatc atataatcaa
     3601 gattagtgtt aaaaaggaga atttatggaa aaaattgaac gcttatttgc caataaccac
     3661 gcctgggcaa ctaagatgaa agatgaacaa tcggattatt ttaaagaatt agcggaacat
     3721 caaaagccaa cctatttatg gattggctgt tccgatagtc gtgtgcctgc tgaaaaactc
     3781 acagggttag gccccggaga attatttgtt catcgaaatg tagcaaatat ggtgattcac
     3841 actgatctta attgtctgtc ggttgttcaa tatgcggtag atgtattaga aattgagcat
     3901 attattatct gtggtcacac taattgtggt ggtatcaatg cggcaattgc acaaaaagaa
     3961 cttggtttaa ttaataactg gttgttacat attcgtgata tctggtttaa acacagccat
     4021 ttactaggca aacttcccta tgaagagcga tcaaatgtct tgacccgatt aaatgttgca
     4081 gaacaagttt ataatttagg tcagtcctct attattaaaa ccgcttggga acagggtaaa
     4141 tccctctcgc tacacggttg ggtatacgat gttacagatg gtttcttgat tgaccaaggt
     4201 gttatggcta ctagccgaga aacattagaa attacctatc gtaatgcaat agctaaaata
     4261 acaaccgaag caaacgaaaa aatagtttta aacgatgaaa attagccatt gtaagcggtt
     4321 aaatttat
//
LOCUS       DANLZC010000165         4305 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.165, whole genome shotgun sequence.
ACCESSION   DANLZC010000165 DANLZC010000000
VERSION     DANLZC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.165"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            6..716
                     /locus_tag="PXE44_002295"
     CDS             6..716
                     /locus_tag="PXE44_002295"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDL5589757.1"
                     /translation="MVALAAIFISIFGTIYALLRLRHPSSVGVVARWFGALHKLVGLE
                     LITRKKPEINYPVVYIGNHQNNYDMVTISSMVAEKTVSIGKKSLIWIPFFGLVYWATG
                     NIFIHREKRSSAIETMNKAGQIVKERNVSVWMFPEGTRSRGRGLLPFKTGAFHLAISA
                     GVPIVPVVCSNLHNKVDLNRSNNGTVICEMLDPIDTSGYSRENVKELMEKCHQIMLAK
                     FEQLNQEVEQLENTAKNA"
     gene            718..2142
                     /locus_tag="PXE44_002296"
     CDS             718..2142
                     /locus_tag="PXE44_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HDL5589758.1"
                     /translation="MKKQITRRQFLQGSALAAGFATVPTPLLAAERPQLNIPPLMDIG
                     RGKPVRLDLRPAQTQFDKGKLVDVWGVNGQYLAPTVRVKSGDFVKLTYLNNLPQKISM
                     NIQGLQASSEMIGTVHRSLDPKSSWSPIVSINQPACTCWYHADTMLNSAFQVYRGLAG
                     LWMIEDEQSKKATLPNKYGVNDIPLILQDQLLNKDGVQVLDKNTSQFFGKRLFVNGQE
                     SPYFNVPRGWVRLRIVNASLSRAYELRLDNEQPLYLIATGLGMLAEPLETESVQLAPS
                     ERVEVLVDLSEGKAVSLISGQKRDIFYKIGQVFADNDELNDNVILELRPEGMAAVLNN
                     KPSLPAFNLEEFNLKISQERRFAIRPADYLINQKRFDPKRIDFTAQKGSVERWYLHST
                     QAVGFTLQGAKFIIETLNRKQYPIKQLAWRDTVWLEAEQEITLLVRFDNTAPEQLPFT
                     FGVSDFMLRDRGTMGQFSVIENGQ"
     gene            2196..3149
                     /gene="accA"
                     /locus_tag="PXE44_002297"
     CDS             2196..3149
                     /gene="accA"
                     /locus_tag="PXE44_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL5589759.1"
                     /translation="MSQEFLDFELPIAELEAKIESLRAVSEQDDKINLDDEIKRLQKK
                     SEELTKKTFANLDAWQISRMARHPNRPYTLDYIEHIFTEFDELAGDRAFADDKAIVGG
                     IARLDGRPVMVIGHQKGRTVKEKVKRNFGMPAPEGYRKALRLMEMAERFNMPIITFID
                     TPGAYPGIGAEERGQSEAIARNLREMSQLSVPVICTVIGEGGSGGALAIGVGDKVNML
                     QYSTYSVISPEGCASILWKSADKASTAAEVMGLTAPRLKELELIDNIVPEPLGGAHRN
                     VEQIAQNLKQRIVEDLAGLDQLNKEDLLERRYQRLMNYGYV"
     gene            complement(3244..3319)
                     /locus_tag="PXE44_002298"
     tRNA            complement(3244..3319)
                     /locus_tag="PXE44_002298"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3284..3286),aa:Thr,seq:tgt)
     gene            3438..4298
                     /gene="pdxY"
                     /locus_tag="PXE44_002299"
     CDS             3438..4298
                     /gene="pdxY"
                     /locus_tag="PXE44_002299"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDL5589760.1"
                     /translation="MKNILSIQSHVVYGYAGNKASTFPMQLLGVDVWALNTVQFSNHT
                     QYGKWGGMVMPKEQIGSIVDGIHNIGELHRCDAVLSGYIGSAEQVAEIIQAFHKVKAE
                     NPNAIYVCDPVMGHPDKGCIVADGVKEGLINLAMAQADIITPNLVELRELTGLTVENF
                     EQAVEAVKVILTKGPKKVLVKHLSRVGKESNKFEMLFANQDGIWHISRPLYQFAKEPV
                     GVGDLTAGLFVANLLNGKSDLEAFEHTANAVNEVMEQTARSGLYELQIIASRDRIVNP
                     QCSYQAVKIA"
BASE COUNT         1285 a          875 c         1014 g         1131 t
ORIGIN      
        1 ttttaattgt tgcccttgcc gctattttca tttctatttt tggcactatt tatgcgttac
       61 tgcgtttacg tcacccaagt agcgtgggtg tggtagctcg ttggtttggt gctttgcata
      121 aattagtcgg gttggaatta attacccgca aaaagccgga aattaactat ccggttgtct
      181 atatcggcaa tcatcaaaac aattacgata tggtaacgat ttcctcaatg gtggcagaaa
      241 agacggtgag tatcggcaaa aaaagcctga tttggattcc gtttttcggt ttggtttact
      301 gggcaaccgg caatattttt attcaccgtg aaaagcgttc cagtgcgatt gaaacaatga
      361 ataaagcagg gcaaatcgtg aaagaacgca atgtgtcggt gtggatgttc ccggaaggta
      421 ctcgtagccg tgggcgtggt ttactgccat ttaaaaccgg tgcattccat ttagccattt
      481 ctgccggcgt gccgattgtg ccggtagttt gctctaattt acacaataaa gtggatttga
      541 accgttcaaa taacggcaca gtaatctgtg aaatgcttga tccgattgat acttcaggct
      601 attcccgtga aaacgtcaaa gaactgatgg aaaagtgcca tcaaattatg ttggcaaaat
      661 ttgaacaact taatcaagaa gtcgaacagt tagaaaacac tgcaaaaaac gcctaatatg
      721 aaaaaacaga tcacccgacg ccaattttta caaggctctg cccttgcggc tggatttgca
      781 acagtgccaa caccgctatt agcagcagag cgaccgcaac tgaacattcc gcctttaatg
      841 gatattgggc gaggaaagcc ggttcggttg gatttacgcc ccgcacaaac ccaatttgat
      901 aaaggcaaat tggttgatgt atggggtgtg aacgggcaat atcttgcccc aaccgtacgg
      961 gtaaaatcgg gggattttgt caaattaact tatctcaata atttaccgca aaaaatctca
     1021 atgaatattc aaggattgca agcctcaagt gagatgattg gcacggtgca tcgttccctt
     1081 gaccccaaaa gtagctggtc gccgattgtt tctatcaatc aaccggcttg tacttgttgg
     1141 tatcacgccg atacaatgct caactcggca ttccaagtgt atcgtggctt agccggcttg
     1201 tggatgattg aagatgaaca gagcaaaaaa gccactttac cgaataaata tggcgtgaac
     1261 gacattccgt taattctgca agaccagctg ctcaacaaag acggcgtaca ggtgttagat
     1321 aaaaacacct cacaattttt cggtaagcga ttatttgtca acgggcagga atcgccttat
     1381 tttaacgtgc ctcgtggctg ggtcagattg cggatcgtca atgcctcact ttcccgtgct
     1441 tatgaattac gcttagataa cgagcagccg ttgtacctaa tcgccaccgg cttaggaatg
     1501 ctggcagagc cacttgaaac cgaatcggtt caacttgcac cgagtgaaag ggtggaagtg
     1561 ttggtcgatt taagcgaagg gaaagcggta tcgctgataa gcggtcaaaa aagagatatt
     1621 ttttacaaaa tcgggcaggt ctttgctgac aacgacgaat tgaatgataa cgtgatttta
     1681 gagcttcgcc ctgaaggaat ggcagcggta ttgaataata aaccgagttt accggctttc
     1741 aacttagagg aatttaacct gaaaatcagc caagagagac gttttgccat tcgtccggct
     1801 gattatttaa tcaaccaaaa gcggtttgac ccaaaacgca ttgattttac cgcgcaaaaa
     1861 ggcagcgttg agcgttggta tttacatagc acacaagcgg tcggatttac gctacaaggt
     1921 gcaaaattca ttattgaaac cctaaaccgc aagcaatacc ctatcaaaca acttgcgtgg
     1981 cgggacaccg tttggctcga ggcggagcaa gaaatcacgc tgttggttcg ctttgataat
     2041 actgcccctg agcaactgcc atttaccttt ggcgtgagcg attttatgct gcgtgataga
     2101 ggtacgatgg gtcaatttag cgtaattgaa aatggacaat aaccgactga taagcgataa
     2161 tgccccataa ttttgcccat aaaaaggaaa atagaatgag ccaagaattt ttagattttg
     2221 aattgccgat tgctgaattg gaagccaaga tcgagtcttt aagagcagtc agcgagcaag
     2281 atgacaaaat taatcttgat gatgaaatca agcgtttaca aaagaaaagc gaggaattaa
     2341 ccaagaaaac ctttgctaat ttagatgcgt ggcaaatttc ccgtatggca cgccacccca
     2401 atcgcccgta cactttagat tatattgagc atattttcac cgaatttgac gaactggcag
     2461 gcgaccgtgc ctttgccgat gataaagcga ttgtcggtgg tattgcccgt ctggacggac
     2521 gtccggtgat ggttatcggt catcagaaag gtcgtaccgt aaaagagaaa gtgaaacgta
     2581 actttggtat gcctgctcct gaaggctatc gcaaagcgtt acgtttaatg gaaatggcag
     2641 aacgctttaa tatgccaatc atcaccttta tcgacacacc gggagcttat ccgggcattg
     2701 gtgcggaaga gcgtggtcag tcagaagcga ttgcccgtaa cctgcgtgaa atgtctcaac
     2761 tttccgtgcc agtaatctgt acggtaattg gcgaaggtgg ctcgggcggt gcattagcga
     2821 tcggtgtggg cgataaggtc aatatgttgc aatattcaac ttactcggta atttcacctg
     2881 aaggctgtgc ctcgattctg tggaaaagtg ccgataaagc ctcaacggca gcagaagtaa
     2941 tgggtttaac tgcccctcgt ttaaaagagc ttgagctgat tgataacatc gtgccggaac
     3001 ccttaggcgg tgctcatcgc aatgttgaac aaatcgccca aaaccttaaa cagcgtattg
     3061 ttgaagattt ggcagggtta gatcaactta ataaagagga tttactggaa cgccgttatc
     3121 aacgcttaat gaattacggt tacgtctaat tcaatttcac cctacaagcg gtaagatttt
     3181 tgtgaaaatt tgcaaaatct tgccatttta cattaaaggg gactcatcag aaaataaagg
     3241 tgatggtgcc gactaccgga atcgaactgg tgacctactg attacaagtc agttgctcta
     3301 cctactgagc taagtcggca tattgaatgg ataagagaac gaggcgggat tatagagatt
     3361 tcttgatgag cttcaagtcc ttgcaacttc aaatgactaa aaaacaacac atattgaaat
     3421 tttaaaggag tgaatgaatg aaaaacatac tatccataca atcgcacgtt gtctacggct
     3481 atgccggcaa taaggcttca acttttccta tgcagctgtt aggtgttgat gtatgggcgt
     3541 taaataccgt acagttttcc aaccataccc aatatggaaa atggggcggt atggtgatgc
     3601 caaaagaaca aatcggtagc attgttgatg gcattcacaa tatcggtgaa ctgcatcgtt
     3661 gcgatgccgt actctctggc tatatcggct cggcagaaca agtagccgaa attattcagg
     3721 cttttcataa agtgaaagcg gaaaatccaa acgcaattta tgtatgcgac ccggtaatgg
     3781 gacaccccga caaaggctgc attgtggcag acggcgtaaa agagggattg atcaatctgg
     3841 caatggcaca agccgacatt atcacaccaa atttagtgga attaagagaa ttaaccggtt
     3901 taacggtgga aaattttgag caagcggtcg aggcagtgaa agtgatttta accaaaggcc
     3961 cgaaaaaagt attggttaaa catttaagcc gagtcggcaa agagagcaac aaatttgaaa
     4021 tgctgttcgc caaccaagat ggcatttggc acatcagccg cccactctac caatttgcca
     4081 aagaacctgt cggtgtaggc gatttaaccg cagggctatt tgtggcgaac ttattaaatg
     4141 gaaaatccga tctagaagcc tttgaacaca ctgcaaatgc ggtcaatgag gtaatggaac
     4201 aaaccgctag atcagggctt tatgagttac aaattattgc ttctcgtgat cggattgtta
     4261 atccacagtg tagttaccaa gctgtaaaga ttgcctaatt aaatg
//
LOCUS       DANLZC010000166         4236 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.166, whole genome shotgun sequence.
ACCESSION   DANLZC010000166 DANLZC010000000
VERSION     DANLZC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.166"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            151..2085
                     /gene="iolD"
                     /locus_tag="PXE44_002300"
     CDS             151..2085
                     /gene="iolD"
                     /locus_tag="PXE44_002300"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL5589761.1"
                     /translation="MQTTRLTVAQALVQFLDNQYLDVDGKEIKFVHGVAAIFGHGNVL
                     GIGQALAQNAGGLTLYQGRNEQGMAHLAMGFAKQNLRQRIIACTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDVFATRQPDPVLQQIEQPYDLSISTNDAFRAVSKYWDRVQRPE
                     QLMSACINAFRVLTDPAETGAVTIALPQDVQAESFDFPNSFLQKRVHRLERTPPTTAM
                     IKQAVTLIQTKKKPLIVCGGGVRYSQAGQILQKFAKQFGIPFAETQAGKSAVVSDHAL
                     NVGGVGETGCLAANRLAKEADLVIGIGTRYTDFTTSSKWIFQNPNVQFLNINVARFDA
                     YKLDGVQLVADAKTALEALSQAIPTDYQAQWENEISQAKADFADEIQRLRAVQYHPDF
                     TPEINDNLDHKAVHNEFIQLTGSQLTQTQVLGIVNETIDNNAIIVAAAGSLPGDLQRT
                     WLSKGEETYHLEYGYSCMGYEVSAAVGVKIARPEQEVYALLGDGSYLMAHTELVTSIQ
                     EGKKINVVLFDNMANGCINNLQIGNGMDSFGTEFRFRNGETNQLNGGLVPIDFAKNAE
                     AYGCKTYRVTNEAELRAALDDAKKQTVSTLIDIKVLPKTMAHGYGCWWHVGVAEVSES
                     ERIQQARQRSEEQLAKARKY"
     gene            2212..3108
                     /gene="iolE"
                     /locus_tag="PXE44_002301"
     CDS             2212..3108
                     /gene="iolE"
                     /locus_tag="PXE44_002301"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011817366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL5589762.1"
                     /translation="MKAENIQLGIAPIGWTNDDLPELGAENTFEQCVSEMALAGYTGC
                     EVGNKYPRDVAVLKHKLDVRGIQICNAWFSTFFVDGKREETIAEFIKHRDFLHAMGAK
                     VIGCSEQSRSIQGTTKSVFKEKPIFNDEDWKRMAEGYNELAKLAAEKGMKVCLHHHMG
                     TGIQTPEEVDRYMAEVNDDVYLLFDSGHLYYSEGCQKAMLGVLEKYIDRIIHVHLKDV
                     RDEVMAEVKAKDLSFLEGVKKGTFTVPGDGVIDFRPIFDILEKHDYKGWMVVEAEQDP
                     ALANPFEYALKGRKYIRETAGV"
     gene            3110..4120
                     /gene="iolG"
                     /locus_tag="PXE44_002302"
     CDS             3110..4120
                     /gene="iolG"
                     /locus_tag="PXE44_002302"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL5589763.1"
                     /translation="MLKVGIIGAGRIGRVHSESISKYVKGAEIKAISDVKITEELTAW
                     AKSMGIPEVYDDYKKILQDPEIDAVLVCSSTNTHAPISIEAAQAGKHVFCEKPVDADP
                     AKIREVLSAVEKAGVKFQVGFNRRFDHNFKAIKDRVAAGDIGEPHVIRVTSRDPEAPP
                     IEYVKVSGGMFFDMTIHDFDMIRYLSGSEVEEVFAVGTVLVNPEIGKAGDIDTAVITL
                     KLKNGAIGVIDNSRKAAYGYDQRAEVFGSKGAIHITNDTGSTAVFSGENGVVAEKPKY
                     FFLERYMQSFADEISCFVDSVVNDKPTLVNGNDGLQPVLIALAAKKSLEENRPVKLSE
                     VA"
BASE COUNT         1245 a          921 c         1034 g         1036 t
ORIGIN      
        1 aaaaataagg caataacttc gcagaaatga gaactggatc acatcttttt ttcgatttcc
       61 ttgctattat aaatgaaata aatttttcat ttgtaaaaat cagtagaaaa ccgaccgctt
      121 gcaaaagcga tgtaagccag aggaggcttt atgcagacta ctcgtttaac ggttgcacag
      181 gcgttagtgc aatttttgga taatcaatat ttagacgtgg acggcaaaga gattaaattc
      241 gttcacggtg ttgccgctat tttcggccac ggcaatgtgc tgggaatagg tcaggcgctt
      301 gctcaaaatg ccggtggttt aacgctctac caaggcagaa atgagcaagg tatggcacat
      361 ttggcgatgg gctttgctaa gcaaaatctg cgtcaaagaa ttattgcctg tacttcctcg
      421 gtcgggccgg gggcggcaaa tatggttacg gctgcggcaa cggcaacagc gaaccgtatt
      481 cctcttcttt tattaccggg cgatgtgttt gcgacccgcc agccggatcc ggtactgcaa
      541 caaatcgaac aaccttacga tttaagcatt agcactaacg atgcgttccg tgccgtcagt
      601 aaatattggg atagagtgca acgccctgag cagttaatga gtgcttgtat caatgcgttt
      661 cgtgtgctga ccgaccctgc tgaaaccggt gctgtcacca ttgctctgcc gcaagatgta
      721 caagcggaga gttttgattt cccgaacagc ttcctgcaaa aacgagtgca tcgcttagag
      781 cgtacaccgc cgacaacagc gatgattaag caagcggtaa cattaattca aaccaagaaa
      841 aaaccgctga ttgtctgcgg tggcggtgtt cgctactcgc aagcgggtca aattctgcaa
      901 aaatttgcta aacagttcgg cattccattt gctgaaaccc aagcgggtaa aagtgcggtg
      961 gtgtccgatc acgcattgaa tgtaggcgga gtgggagaaa ccggttgttt agcggcaaac
     1021 cgtctggcaa aagaagcgga tttagtgatt ggcattggta cacgctacac cgacttcacg
     1081 acttcttcca aatggatttt ccaaaaccca aacgtgcagt ttttgaacat taatgtggca
     1141 cgttttgatg cgtacaaatt agacggcgtg caactggttg ccgatgcaaa aactgcatta
     1201 gaggcgttaa gccaagccat tcctactgat tatcaagctc aatgggagaa tgaaatttca
     1261 caggcgaaag cggattttgc tgatgaaatt caacgcctgc gcgctgtgca atatcaccct
     1321 gatttcaccc ctgaaattaa cgataatctt gatcataaag cggttcacaa tgaatttatt
     1381 cagctcaccg gttcccagct cacccaaacc caagtgctgg gtattgtgaa tgaaaccatt
     1441 gataacaatg cgattattgt tgccgccgcc ggaagtttgc cgggtgattt acaacgcact
     1501 tggttaagta aaggtgaaga gacttaccac cttgaatatg gctactcctg tatgggctat
     1561 gaagtgagtg cggcagtcgg cgtgaaaatt gcccgccccg aacaagaggt ctatgccttg
     1621 ctgggggacg gctcttactt aatggcacac accgaattag tgacctcaat tcaagagggc
     1681 aaaaaaatca acgtggtgct gttcgacaat atggcaaacg gctgtatcaa caacctgcaa
     1741 atcggcaacg gtatggacag tttcggtacg gaattccgct tccgcaatgg cgaaactaac
     1801 caactcaacg gcggtttagt gccgattgat tttgccaaaa acgccgaagc atacggctgc
     1861 aaaacctatc gggtaactaa cgaagccgaa ttgcgtgccg cccttgatga tgcgaaaaaa
     1921 cagaccgttt ctaccttaat cgatattaaa gtgttgccga aaacaatggc tcacggctat
     1981 ggctgctggt ggcacgttgg tgtcgcggaa gtgtcagaga gcgagcgtat tcaacaagcc
     2041 cgccaacgct ccgaagagca gttagctaag gctcggaagt attagttaaa aaaacaactc
     2101 cccactttga gctacgaggg gagaatatca agcggtcaaa tttgcaaaat ttttgataaa
     2161 aataaccgct tgcatccaaa tttatctcta taaatcaaac gaggtttctc tatgaaagct
     2221 gaaaacattc aattaggcat tgccccaatc ggttggacga acgatgacct gccggaactg
     2281 ggagcggaaa acactttcga gcagtgtgtg agtgagatgg cacttgccgg ctacacgggc
     2341 tgtgaagtgg gtaataaata tccgcgtgat gttgccgtgt taaaacacaa actggacgtg
     2401 cgtggcattc aaatctgtaa tgcgtggttc agcaccttct ttgtggacgg taaacgggaa
     2461 gagaccattg ccgagttcat caaacatcgt gatttcttgc atgcaatggg ggcaaaagtg
     2521 atcggctgtt ccgaacagag ccgtagtatt caaggcacaa ccaaatctgt gttcaaagaa
     2581 aaaccgattt tcaacgatga agattggaaa cgtatggcag aaggctataa cgaattggcg
     2641 aaacttgccg ccgaaaaagg aatgaaagtc tgcctgcatc accatatggg aaccggtatt
     2701 caaacacccg aagaggtcga tcgctatatg gcagaagtga atgacgatgt ctatctgttg
     2761 ttcgattcag ggcatttgta ctattccgaa ggctgccaaa aagcgatgct cggcgtactg
     2821 gaaaaatata tcgaccgtat tatccacgtt cacttaaaag atgtgcgtga tgaagtcatg
     2881 gcggaagtaa aagcaaaaga tttaagcttt ttagaaggcg tgaaaaaagg cacctttact
     2941 gtaccgggcg atggtgtcat tgatttccgt ccgattttcg atattttaga aaaacacgac
     3001 tacaaaggct ggatggttgt agaagccgaa caagatccgg cacttgccaa cccgtttgaa
     3061 tacgccctca aaggcagaaa atatatccgt gaaaccgcag gagtttaata tgttaaaagt
     3121 aggtattatt ggtgcaggcc gtattggacg tgtgcattct gaaagtatta gcaaatatgt
     3181 caaaggtgct gaaatcaaag caatctctga tgtaaaaatt accgaagaat tgaccgcttg
     3241 ggcaaaatcg atgggtattc cggaagttta tgatgactat aaaaaaatcc tgcaagatcc
     3301 ggaaattgat gctgtattgg tctgctcttc caccaacact cacgctccga tttcgattga
     3361 ggcagcacag gctggcaaac acgttttctg tgaaaaaccg gtagatgcag acccagctaa
     3421 aatccgtgaa gtattgagtg cagtcgaaaa agccggtgta aaattccaag tgggctttaa
     3481 ccgccgcttc gaccacaact tcaaggcgat taaagaccgt gtggcggcag gcgacatcgg
     3541 cgaaccgcac gttatccgag taacctctcg tgatccggaa gccccgccaa tcgaatatgt
     3601 gaaagtctcc ggcggaatgt tctttgatat gaccattcac gacttcgata tgattcgtta
     3661 tttatccggc agcgaagtgg aagaagtgtt tgcagtcggc accgtattag taaaccctga
     3721 aatcggcaaa gcgggcgata ttgatactgc cgttatcacg ttgaaactga aaaacggagc
     3781 aatcggcgta atcgacaaca gtcgcaaagc tgcctacggc tacgaccaac gtgcggaagt
     3841 gttcggctcg aaaggggcaa tccatatcac taatgatacc ggctcaactg ccgtattctc
     3901 aggtgaaaac ggcgtggtgg cggaaaaacc aaaatacttc ttcttagaac gttatatgca
     3961 atctttcgcc gatgaaatca gctgtttcgt agattctgtg gtaaacgaca aaccaacgtt
     4021 agtaaacggc aatgacggct tacaaccggt acttattgcc ttagctgcga aaaaatcact
     4081 ggaagaaaac cgaccagtga aattgagtga agtggcgtaa ttgatcataa atacaagcgg
     4141 tcggttttac tcgctacact cgcacttcgt gccatcggca tagccgatgt tcaaacgcaa
     4201 gcattttgca gaaaaatttg taaaactgac cgtttg
//
LOCUS       DANLZC010000167         4236 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.167, whole genome shotgun sequence.
ACCESSION   DANLZC010000167 DANLZC010000000
VERSION     DANLZC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.167"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(3..1409)
                     /locus_tag="PXE44_002303"
     CDS             complement(3..1409)
                     /locus_tag="PXE44_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDL5589764.1"
                     /translation="MKLTKIVLSVVAVIGAVAVGGSWYTGKQVEEKYQELVVQANNQL
                     KQLSGEFGANIELKDVQIDRHFFISNAKYRLEVDLGNGKKLDFVGNDKLHHGPLPLNR
                     LAKLNLAPVMMSAESKLQAPEALKKLMGEHIGTGFSNISYSGDVEGHFDISPIKHSDE
                     TGHIETTPIKVQYSYDQKLKGVDSTVELESLKIKAEEFDFQIQGIVYDVQTENNESYP
                     NLTLGKGSGKINAIEFKSKDGKLSQIKDVVVKGDNLLKGDRLISTGDLEAATFNIEGV
                     EMGKLKMDMAYDLDAKLTNDITPLLSNPQTLENEKTGELLLQLLAKSFKFHINNFSLE
                     NSKGKVDLALLLNMAQFDPQNLGNMQAVLQALSTSKFTSNINRQYAEDIIRQVSIVTE
                     KLGEEEAKAFAKQQVDAVFLNAGVEQYGLRKVDDNNVKIELTIDNGKVNLNGRELTED
                     ELQMALFMIVMGAGSLGQ"
     gene            complement(1459..2265)
                     /gene="trpA"
                     /locus_tag="PXE44_002304"
     CDS             complement(1459..2265)
                     /gene="trpA"
                     /locus_tag="PXE44_002304"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDL5589765.1"
                     /translation="MSRFDTLFTALKAKNEGAFVPFVTLCDPGFERSFEIIDTMIKNG
                     ADALELGFPFSDPLLDGPVIQAANKRALDGGYSTDSCFELIAKIRAKYPDIPIGLLLC
                     ANLIFVPTAEVFFQRCQQSGVDAVLIADLPVFAAQEFVPTAEKYGIQPVFICPPNADQ
                     NTIERIAQLTQGYTYLVSRAGVTSAENQAHASNLDNLVESLKQAGAAPILQGFGIAKP
                     EQVKEALRLGADGAISGSAIVKIIERNLADQANLLKELAEFVQVMKAATK"
     gene            complement(2267..3457)
                     /gene="trpB"
                     /locus_tag="PXE44_002305"
     CDS             complement(2267..3457)
                     /gene="trpB"
                     /locus_tag="PXE44_002305"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDL5589766.1"
                     /translation="MSDTLLNPYFGEFGGMYVPEILVPVLKELEQAFVDAQQDPTFQA
                     EFADLLKNYAGRPTALTLCRNLTKGSKTKLYLKREDLLHGGAHKTNQVLGQILLAKRM
                     GKTRIIAETGAGQHGVATALACAMLGMPCVVYMGAKDVERQSPNVFRMRLMGAEVIPV
                     QKGSCSLKDACCEAMRDWAANYDTTHYLIGTAAGPHPFPTMVREFQKMIGEEIKRQIL
                     AKEDRLPDAVIAAVGGGSNAIGMFADFIEEQSVKLIGVEPAGKGIETGEHGAPLKHGT
                     TGIYFGMKSPIMQDKDGQIEESYSISAGLDFPSVGPQHAYLNATGRAEYVSITDAEAL
                     EAFQALAKNEGIIPALESSHALAYALKMIKQNPEKEQLLVVNLSGRGDKDIFTVDKIL
                     NGGE"
     gene            complement(3469..4221)
                     /locus_tag="PXE44_002306"
     CDS             complement(3469..4221)
                     /locus_tag="PXE44_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5589767.1"
                     /translation="MKIALVTGVTAGFGLAICKLLLQSGYKVIGTGRRAERLEELHLQ
                     FGDKFLPLAFDIQQPDEVEKALNSRPKAWQQIDLLVNNAGLALGLEPAQKADLNDWYQ
                     MIDTNIKGLVTMTRLVLPEMVERKSGHIINIGSIAGTYPYPGGNVYGGTKAFVKQFSL
                     NLRADLAGTKVRVSNVEPGLCGGTEFSNVRFKGDDEKAAKVYENVQFVRPEDVANIVL
                     WLNQQPEHVNINRVEVMPVAQTSSALNVARNI"
BASE COUNT         1165 a          990 c          754 g         1327 t
ORIGIN      
        1 gattattgcc cgagtgagcc tgctcccatc acaatcataa acagagccat ttgaagttca
       61 tcttcggtca gttcacgtcc gtttaaattc acttttccgt tatctatagt taattcaatc
      121 ttcacattgt tgtcatctac ttttcttaat ccgtactgtt caactcccgc attgagaaaa
      181 acagcatcaa cttgttgctt ggcaaatgct ttcgcctctt cttcgcctaa tttttctgta
      241 actattgaga cttggcgaat aatatcttcc gcatattgac ggttgatatt tgaagtgaat
      301 ttacttgttg aaagggcttg caataccgct tgcatattac caagattttg tgggtcaaat
      361 tgagccatat tcagtaataa agccaaatca actttgcctt tgctgttttc aagtgagaaa
      421 ttattaatat ggaatttaaa tgattttgct aatagttgta ataataactc gcctgttttt
      481 tcattttcta atgtttgagg attagataat aaaggcgtaa tatcattggt taatttagca
      541 tctaaatcgt atgccatatc catttttaat ttacccattt caacaccctc aatgttgaaa
      601 gtggctgcct ctaaatctcc ggtagaaatt aaacgatcgc cttttaatag attatcgccc
      661 ttcaccacaa catctttaat ttgagataat ttgccatctt tagacttaaa ctcaatagcg
      721 ttaatttttc cactaccttt gcctaaagtt aaattaggat aactttcatt attttctgtt
      781 tgaacatcat aaactatacc ttgaatttga aaatcaaatt cttctgcttt tatcttaaga
      841 ctttctagct caacagtgct atcaacccct tttaattttt ggtcgtagct atattgcact
      901 ttgattggcg tggtttcaat atgacctgtt tcatcactat gtttaatcgg ggaaatatca
      961 aaatgccctt ccacatcgcc cgaatagctg atattagaaa aaccggtgcc aatatgttca
     1021 cccatcaatt ttttaagggc ttcaggagct tgtaatttgc tttccgcact catcattaca
     1081 ggagctaaat taagtttcgc taaacgatta agcggtagcg gaccgtgatg aagtttatca
     1141 ttaccaacaa agtccaattt cttaccatta cccaaatcca cttctaaacg atatttcgca
     1201 ttagagataa agaaatggcg atcgatctgc acatctttaa gctcaatatt cgcaccaaat
     1261 tcgccactca gttgtttaag ttggttatta gcttgaacaa ccaattcttg atatttttct
     1321 tcaacctgct tacctgtgta ccaacttccg ccaactgcaa cagcaccaat taccgctaca
     1381 actgaaagaa caatttttgt taatttcatt ctgtttcctt ttattttcaa aaaaatttaa
     1441 aaaatgaccg cttgtagttt acttcgtagc cgctttcatc acttgtacaa attcggcaag
     1501 ttctttgagc aaattagctt ggtcagctaa attacgttca ataattttca caatcgctga
     1561 gcctgaaatc gccccgtctg ccccaagcct taacgcctct tttacctgtt caggtttggc
     1621 tatgccaaaa ccttgtaaga taggggcagc tccggcttgt tttaaacttt ccactaaatt
     1681 atccaaatta cttgcgtgag cttggttttc agcactagtt acaccggcac gggaaactaa
     1741 ataagtgtag ccttgggtta attgagcaat ccgttcaatg gtgttttgat cagcattcgg
     1801 cgggcaaata aacaccggct gaattccata tttttcagcg gttggcacaa actcttgagc
     1861 agcgaaaaca ggcaaatcag caatcaacac cgcatcaact ccactttgtt ggcaacgttg
     1921 gaaaaatact tctgcggttg gcacaaaaat taaattggca cacagtagta aaccgatagg
     1981 aatgtctgga tatttagctc gaattttggc aatcagctca aaacagctat cagtgctata
     2041 gccaccatct aaagctcgtt tattcgctgc ttgaatcacc ggcccgtcta agagggggtc
     2101 agaaaacgga aagcctagct ctaaagcatc agccccgttt ttaatcatcg tgtcgataat
     2161 ctcaaaactg cgttcaaaac caggatcgca taatgtcaca aacggcacaa aagccccttc
     2221 gttttttgct tttaatgcag taaatagcgt atcaaagcgg ctcattttac tctcctccat
     2281 ttaaaatttt atccacggtg aaaatgtctt tatcgcctcg acccgataaa ttcaccacta
     2341 ataactgctc tttttcaggg ttctgcttaa tcatttttaa cgcataggct aaggcgtggg
     2401 aagattctaa cgccggaata atcccctcat ttttggctaa tgcttggaat gcctctaaag
     2461 cctctgcatc ggtaatcgat acatattcag ctcgccctgt cgcatttaaa taagcgtgtt
     2521 gcggcccgac agaagggaaa tctaagccgg ctgaaatgga gtaagactct tcaatttgcc
     2581 cgtctttatc ctgcataatc ggcgatttca taccgaaata gatccccgtt gtgccgtgtt
     2641 tgagcggtgc accgtgttca ccggtttcaa ttccttttcc tgccggctca acaccgatca
     2701 atttgacgga ttgctcttcg ataaaatctg caaacatacc gatagcattt gagccaccgc
     2761 ctacggcagc aatcaccgca tcaggcaagc ggtcttcttt tgctaaaatt tgacgtttga
     2821 tctcttcgcc aatcattttc tgaaattcac gcaccattgt cgggaacggg tgcgggccgg
     2881 ctgcagtgcc gattaggtaa tgggtagtat cataatttgc cgcccaatcc cgcattgcct
     2941 cgcaacaggc atctttgagc gagcaagagc ctttttgtac cggaatcacc tccgctccca
     3001 ttaaacgcat acggaataca tttggcgatt ggcgttctac atcttttgcg cccatataca
     3061 ccacgcaagg cattcctaac atcgcacacg ctaatgcggt tgccacaccg tgctgtcctg
     3121 ctcctgtttc ggcaataatg cgggttttac ccattcgttt agcgagtaag atttgcccta
     3181 gcacttggtt ggttttatgt gccccaccgt gtaaaagatc ttcacgtttt aaatagagtt
     3241 tggttttact gcctttggtt aaattacgac aaagggttaa agcagtcggg cgaccggcat
     3301 agtttttgag taaatcggca aattcagctt ggaatgttgg gtcttgctga gcatctacaa
     3361 aggcttgttc taattctttt agcaccggca ctaaaatttc cggcacatac ataccaccaa
     3421 attcaccaaa ataaggatta agaagtgtgt ctgacataat atttcctttt aaatatttct
     3481 cgctacattt aaggcagaag aagtttgagc caccggcatc acttcaaccc gattaatatt
     3541 aacgtgttca ggttgctgat tcagccaaag cacaatattt gccacatctt ccgggcgcac
     3601 aaactgcacg ttttcataaa ctttagccgc tttttcatca tcgcctttaa agcgtacatt
     3661 tgaaaattcc gtaccgccac atagccccgg ctcaacattg gaaacacgca ctttagtgcc
     3721 ggctaaatct gcccgcaaat ttaggctaaa ctgtttcacg aaggctttag taccaccata
     3781 tacattaccg cccgggtaag ggtaagtgcc ggcaatcgag ccgatattaa taatatgccc
     3841 acttttccgc tccaccattt caggcaaaac taagcgtgtc atcgttacca atcctttaat
     3901 attggtatcg atcatttgat accaatcgtt caaatctgct ttctgtgccg gttctaagcc
     3961 gagtgcaagg cctgcattgt ttactaataa atcaatttgt tgccacgctt tagggcggct
     4021 attcagtgct ttttccacct catcgggctg ttgaatatca aatgcaagcg gtagaaattt
     4081 atcgccaaat tgtaaatgca gttcttctaa acgctctgcc cgtctgcccg tgccaattac
     4141 tttataaccc gattgaagca ataatttaca aattgccaac ccaaatccag cagttactcc
     4201 tgtgactaat gctattttca tcttttcacc ctcaaa
//
LOCUS       DANLZC010000168         4231 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.168, whole genome shotgun sequence.
ACCESSION   DANLZC010000168 DANLZC010000000
VERSION     DANLZC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.168"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            74..1531
                     /locus_tag="PXE44_002307"
     CDS             74..1531
                     /locus_tag="PXE44_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589768.1"
                     /translation="MRSTFKLTLAALAVSIVLSGCKSSQGPSIVADSPPPKEITATDG
                     NDKEKTDNKEKKSAQPNKSDPQPDKPANAKDPVKPDAETNKPSKKDKEYTPPATEKID
                     NSSVQPDKMVPNPIENKENDSNKPNPKESDSDNNDKVTEGNKPNAPTETVNKDKEAEK
                     SKKENKEDSSNNTPNINFDGAKPIDTDSKGSPYRVINLNPRKSVVNASAMQKVDFDSF
                     TDESVENSPFVSSDTEKEYNNTLVFGLGAGDKDSYFKNLNQKGDKHLGIHSGVYEDAN
                     LEGNTKKINYLYVNQPYSSYGALFTDQKDSHLFNVRLTAGRDDVKENGDSYAEYGVFT
                     QSGKEAKWNDNLIGDATYKGQAIARVIRQVNGEQVAGIPQFDGDVTLNLHLSKEWNES
                     KLNGKIDSQSVGVITLKESEIANPTALRSNIGFGEEVIVENKENLVGDYAVDFTGPQL
                     EEAVGVVELENEAETFSENEVIKYNAVFGVSKAAK"
     gene            1595..3007
                     /locus_tag="PXE44_002308"
     CDS             1595..3007
                     /locus_tag="PXE44_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5589769.1"
                     /translation="MKKIALLALFPMMLFARDPNTTQAYIEHTAQPTTTPQRVKIEKS
                     VPIFNETELRANQLLTEKLLIQAIYSRRLANMEKLTAIYRDFTHIDPVLLRYAQGKIA
                     LLKGDYAQAIQHFQTILAKQPKLNTVRIELAIALLYDQQLNQAQAQFEKAKSAEHLPP
                     KVHALIEQYQQHLAKKQEWKFSFSANYVRDGNINRTSKQPNIEQTGYVKQPNLLPQTA
                     HGVHYAFGLQRDFNLFDSHYVSFDNQTQGELYWDNHDYDEITNRTLFGYAYKKSDQTT
                     RLLPFYEKRWKHHRSERWTNGIRLEHAQWFSPHWQLATALEYGKKRYFDLPIYNGSYK
                     LASATLLWLRNPQQFFFVGTDFNQDKTQLKQYSSDTKGIRFGWQREWQALELSSRLTL
                     GYTDRRYKDTAILGGKLNLGRIRHDRVQSANLTLWKRDWHWLGLMPKLQFSYAKHRSN
                     LPSLYAYTDKNINLIIESRF"
     gene            complement(3257..3580)
                     /locus_tag="PXE44_002309"
     CDS             complement(3257..3580)
                     /locus_tag="PXE44_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
                     /protein_id="HDL5589770.1"
                     /translation="MQKAYQIYTHSEREKVTEAKLELGKVETRLRNEGKTKEEIAQHP
                     EYLQLKRDLNDKQQTFDNAYGTGSKTKRAVDAVTAALQGLAAKDSGQAAVGLASPYLN
                     EQIKK"
     gene            complement(3549..4076)
                     /locus_tag="PXE44_002310"
     CDS             complement(3549..4076)
                     /locus_tag="PXE44_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDL5589771.1"
                     /translation="MRDKNKERQQTLERYQELYDQDPELRVIAANSEGDLDLLQETFF
                     VKGELTITDSTPKEKWNWLHMCNLNSYSPSPLSVVEFYIKHGVDVNAQDCYGMTPLHY
                     AMRAKNVDAAIALLNAGADPNIENKDQVIPLSMIGAVPKEIELIEHDISEVKETQELV
                     KGIGEIAEGLSDLHA"
     gene            complement(4078..>4231)
                     /locus_tag="PXE44_002311"
     CDS             complement(4078..>4231)
                     /locus_tag="PXE44_002311"
                     /inference="COORDINATES: protein motif:HMM:NF025767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589772.1"
                     /translation="KQFNDYVNARPENFRLENISENRSHKNEMPGKDNIKSIIDDMKK
                     FQQIGK"
BASE COUNT         1362 a          890 c          772 g         1207 t
ORIGIN      
        1 taaattttcc ctattgccaa acaggaaata tatccattaa aattttatgg atttaatctg
       61 acggagacaa aatatgcgtt ctacattcaa acttactctt gcagctttag ctgtttctat
      121 tgttttaagt ggctgtaaat ctagccaagg tccatctatt gttgcagaca gccctccacc
      181 taaggaaata acagctactg atggcaacga taaagagaaa accgataaca aagagaaaaa
      241 gtctgcgcaa ccaaataaat ccgatcctca accggataaa cctgctaacg caaaagaccc
      301 agtaaaacct gatgcggaga caaataaacc aagtaaaaaa gataaagaat atacaccacc
      361 tgctactgaa aaaatcgata actcatcagt acagccggat aaaatggtgc ctaatcctat
      421 cgagaataag gaaaacgaca gtaataaacc taaccccaaa gaaagcgata gcgataataa
      481 tgacaaagtc actgaaggca ataaaccaaa tgccccaaca gaaacagtaa ataaagataa
      541 agaagctgag aaaagtaaaa aggagaataa agaggactca agcaataata ctcctaacat
      601 taattttgac ggggcaaagc ctatagatac agatagtaaa ggtagcccgt accgagtaat
      661 aaacctaaat ccgagaaaaa gcgtagtaaa tgcaagtgct atgcaaaaag tagatttcga
      721 ctcttttacg gatgaatctg ttgaaaattc accatttgtc agtagcgata ctgaaaaaga
      781 atacaataat acacttgttt ttgggcttgg tgccggagat aaagattctt attttaagaa
      841 cttaaatcaa aaaggggata aacatttagg tattcatagt ggcgtgtatg aagatgcgaa
      901 tttagaaggt aatactaaaa aaattaacta tctctatgta aatcaacctt attcgagcta
      961 tggtgcttta tttacggacc aaaaagacag ccatttattc aacgttcgtc tgaccgctgg
     1021 tagagatgat gtaaaagaaa atggagatag ttatgccgaa tatggtgtat tcacacaatc
     1081 aggaaaagaa gctaaatgga atgataacct gattggtgat gccacttaca aaggtcaagc
     1141 tattgctcgc gttattcgtc aggtaaatgg ggaacaagtc gcaggtattc ctcaatttga
     1201 tggtgatgtt acattaaatt tacacctttc caaagaatgg aatgaaagta aactaaacgg
     1261 caaaattgat agccaaagtg ttggggtaat tacacttaaa gaaagtgaaa tagctaaccc
     1321 taccgcactc cgctcaaata ttggttttgg agaagaagtc atagttgaga ataaagagaa
     1381 tcttgtgggt gattacgctg tcgattttac cgggcctcag cttgaagagg cggtaggcgt
     1441 tgttgaatta gaaaacgaag ccgagacatt ctctgaaaat gaagtcatta aatacaatgc
     1501 ggtatttggc gtaagcaaag cggcgaaata gtattttttg tatcttacaa agggctgtta
     1561 agccctttta tttttattat tttaaggata aaaaatgaaa aaaattgcct tactggcact
     1621 tttcccaatg atgttatttg cccgagatcc taatactaca caggcttata ttgagcatac
     1681 tgcacagcca actacaaccc cccaaagggt caagatcgaa aaatcagtgc ctatttttaa
     1741 cgaaactgaa ttacgtgcta atcaattatt gaccgaaaag ctacttatcc aagctattta
     1801 tagtcgtagg cttgctaaca tggaaaaatt aactgcgatt tatcgggatt ttacccacat
     1861 tgacccggtt ttactgcgtt atgcacaagg taaaattgcc ctactgaaag gcgattacgc
     1921 acaggcaatt cagcacttcc aaaccattct tgccaaacaa cccaaactaa acactgtaag
     1981 aattgagctg gcgattgctc tattgtacga tcagcaactt aatcaagccc aagcacaatt
     2041 tgaaaaagcc aaaagtgccg aacatctacc gcctaaagtt cacgcattaa ttgagcaata
     2101 tcagcaacat ctggcaaaaa aacaggaatg gaaatttagt ttttcagcca attatgtgcg
     2161 agacggcaac attaaccgaa cctcgaaaca gccgaacatt gagcaaaccg gctatgtaaa
     2221 acaacctaat ttgttgcccc aaaccgcaca tggtgttcac tatgcgtttg gattacagag
     2281 agatttcaac ctgttcgact cacactatgt gagttttgac aaccaaaccc aaggtgaact
     2341 ttattgggat aaccacgatt atgatgaaat taccaatcgc accttgtttg gatacgctta
     2401 caaaaaatcc gaccaaacca cccgcttgtt accgttttat gaaaaacgct ggaaacatca
     2461 ccgttcggaa cgctggacaa acggaattcg acttgaacac gcacaatggt tttcaccgca
     2521 ttggcaactc gccaccgcat tggaatacgg caaaaaacgt tattttgatt tgccgattta
     2581 taacggcagc tacaaacttg cctctgccac attgctttgg ttacgcaatc cacaacaatt
     2641 tttctttgtc ggcactgatt tcaatcaaga caaaactcag ttaaaacaat atagctctga
     2701 tactaaaggc atacgctttg gctggcaacg agaatggcaa gccctcgagc tttccagtcg
     2761 cctcacgctt ggctatactg acagacgtta caaagacacc gctatattgg gaggaaaact
     2821 taatcttggc agaatccgcc atgaccgtgt gcaaagtgct aacctcacgc tttggaaacg
     2881 agattggcac tggctcggcc tgatgccgaa gctgcaattt agttacgcca aacaccgtag
     2941 caacctgccg agcctctatg cctacaccga taaaaacatc aatttaatta ttgaaagtcg
     3001 gttctagctc cataaaaaca acaagcggtt aaaattccta gaaattttgc aaattgcaaa
     3061 aaatgtagaa aatttaaccg cttgtatcta tactgcagct tcttttcctt cagtataaat
     3121 taatatacac cgttgcaaca ataatggctg ctccctgtca tcaccttgaa ctgaaaattg
     3181 ctcaaggtgg atatgactat cgcagccgct tccaccgcac ccagtaatgt atgggcaagc
     3241 aggttggctt gggtatctac tttttaattt gttcgttgag atatggcgaa gctaagccta
     3301 ctgctgcttg tcctgaatct ttcgccgcta aaccttgtaa ggctgccgtc accgcatcca
     3361 ccgcacgctt ggttttgctg cccgtgccgt aggcgttatc aaaagtctgc tgtttatcgt
     3421 ttaaatcccg ttttaattgg agatattcag gatgttgagc gatttcttct tttgtttttc
     3481 cctcatttct caaacgggtt tccactttgc ccaattccag cttcgcttcc gtcacttttt
     3541 cacgctcact atgcgtgtaa atctgataag ccttctgcaa tctcgcctat gcccttcacc
     3601 agctcctgtg tttctttcac ctcactaata tcgtgttcta ttaactctat ctctttagga
     3661 actgctccaa tcatagacaa tggaataact tgatctttat tttcaatatt cggatctgcc
     3721 cccgcattta ataatgctat cgctgcatca acattctttg ccctcattgc ataatgtaaa
     3781 ggggtcattc cgtagcaatc ttgggcatta acatctaccc catgtttaat gtaaaattcg
     3841 actacagaaa gaggagatgg tgaataacta tttaagttac acatatgtag ccagttccat
     3901 ttttcttttg gtgtgctatc cgtaatagtc agttcacctt ttacgaaaaa tgtttcttgt
     3961 aaaagatcga gatcgccttc tgagtttgct gcaataactc ttaattcagg atcttgatca
     4021 tataattctt gataacgttc taatgtctgc tgacgttctt tattcttatc tctcatttta
     4081 ctttcctatt tgttggaatt ttttcatgtc atcgattatt gatttaatat tatctttccc
     4141 cggcatctca ttcttatgac tccgattttc agatatattt tctaatctga aattctccgg
     4201 tctcgcatta acataatcat taaattgttt t
//
LOCUS       DANLZC010000169         4187 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.169, whole genome shotgun sequence.
ACCESSION   DANLZC010000169 DANLZC010000000
VERSION     DANLZC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.169"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(92..2281)
                     /gene="yccS"
                     /locus_tag="PXE44_002312"
     CDS             complement(92..2281)
                     /gene="yccS"
                     /locus_tag="PXE44_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDL5589773.1"
                     /translation="MNKFLRYLQHSWLSENVIKTLPMFISLNIAAVSIWQLGISDFAM
                     PLMLGIIAGGLVDLDNSLIGRAKNLIISLVAFSLSSIGASVSLHLGWLFVPVAVVCTF
                     LLVMLGAIGQRYSTIAFGTLIVAVYNSLAYTPEVLWYHNVVMILLGTLLYGLVGILVY
                     LIFPNRTVQENLASAYLALSHYLLAKSAYFDPDDDDLAAKQLALAKANSEVMAAFDKT
                     RVALFYRLSRQHKQQRTQRMLRYYFTAQDILERASSSHYQYHELFQQLSNSDLMFRFQ
                     RVLELQAAACQKIAMSLRHREVYEHGVRGEKALQGLLNSLNYHQERGLKSAYRWRAIA
                     ENLGNIERQLAQIEQGSSSGEHIEPLSKNFTKSHRLIAENITGLSNISQAIKAHLTFE
                     SQLFRHAVRLSIVVLLCSAIVPILGFDSKGYWILLTAIFVCQPNYTATKTRLIQRVIG
                     TIFGVLVGGSFELFNVALSLEAQLGLIVLTGSLYTFFRLVHYGFSTFYITVLVMISLD
                     IAGVSLESGILMRMLDTLLGTAIAWLAVAFLWPDWKYLNLQRNLHNGLKASGQYLRHI
                     IAQLQFGYNEQLPYRIARRDVHNHLSALSNAITNMYSEPQKYANDLTFAPKLLGIAYT
                     LLGYISTLGAYRIASREINQQVDFSAMFFSHAREVANIIGQIASESRSFAKLSEKLTA
                     IDQDLTQFEQENQQKQNDLALVLIQQLRLIVQLLPQLNSILQLKSVR"
     gene            complement(2325..2783)
                     /locus_tag="PXE44_002313"
     CDS             complement(2325..2783)
                     /locus_tag="PXE44_002313"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL5589774.1"
                     /translation="MKTTVRTIAKQKQIALVAHDSCKQDLIRWTQTHQAQLASHKLYA
                     TGTTGHLLARETGLEITSLLSGPMGGDQQLGGLIAEKKIDLLVFFWDPMNAAPHDPDV
                     KALMRIATVWNIPVAINETTANFLLNADLFNQAISVNVPDYDGYLAERLG"
     gene            2909..4183
                     /gene="thrC"
                     /locus_tag="PXE44_002314"
     CDS             2909..4183
                     /gene="thrC"
                     /locus_tag="PXE44_002314"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDL5589775.1"
                     /translation="MNLYNIKHPEEQVNFAQAVRQGLGKNQGLFFPEVLPKLDSIDEL
                     LDLPLVKRSQKILSALIGEELPQETLNAMVKNAFTFPAPLAKVNDDIYALELFHGPTL
                     AFKDFGGRFMAQALANVRGDGKITILTATSGDTGAAVAHAFYGLENINVVILYPKGKI
                     SPLQEKLFCTLGGNIRTVAIESDFDACQALVKQAFDDAELRQAIGLNSANSINISRLL
                     AQICYYFEAVAQLPKEKRDNVVVSVPSGNFGNLTAGLIAKTLGLPIKRFIASTNANDT
                     VPRYLQSGKWEPNATVATLSNAMDVSRPNNWPRVEELFKRNGWSLSELGSAALNDAET
                     EAALKAQYAEGYLCEPHGAIAYQSLKDQLQQGETGIFLCTAHPAKFKESVEHILAIEL
                     PLPEALDKHNKLPLLSDEMAADFATLRAYLLK"
BASE COUNT         1226 a          959 c          878 g         1124 t
ORIGIN      
        1 aaacaaaaca tcaacaaaaa attaacaaaa ccataatatt tttattgatt ttgtgaagca
       61 tgtcacattt tatgatttaa ttataggctc actaccttac actcttgagc tgtaatatac
      121 tatttaactg cggtaataac tgcacgatca gtctgagttg ttgtatcaac actaaggcga
      181 ggtcattttg cttttgctga ttttcttgct caaattgggt gaggtcttgg tcgatagcgg
      241 tcaatttttc cgataatttt gcaaatgaac ggctctctga ggcaatttgc ccgatgatgt
      301 ttgccacctc tctggcgtgt gagaagaaca ttgcggaaaa atccacttgt tgattaatct
      361 ctcggctagc aatacggtaa gcccctaagg tggaaatata acccagcaag gtgtaagcaa
      421 tacctaacaa ttttggggcg aaggttaaat cgttcgcata tttttgtggt tcgctgtaca
      481 tattggtaat cgcattactc agtgccgata aatggttatg cacatcacgg cgggcaatac
      541 ggtaaggcag ttgctcgtta taaccaaact gcaactgggc gataatatgg cgtaaatatt
      601 gcccactggc tttcaagccg ttatgtaagt tacgttgtaa atttaaatac ttccaatccg
      661 gccataagaa tgccactgcc agccacgcaa tcgctgtacc tagtaaggta tcaagcattc
      721 gcattaagat gccgctttca aggctcacgc ctgcaatatc aaggcttatc attaccaaca
      781 cagtgatata aaaggttgaa aagccataat gtaccaagcg gaaaaaggta taaagcgaac
      841 cggtcagtac gattagccca agctgggctt cgaggctgag cgccacgtta aacagttcaa
      901 aagagccacc aaccagcacg ccgaaaattg tgccgatcac acgctgaatc agtcgggtct
      961 tagtagcagt gtaattcggt tggcagacaa aaatggctgt gagtaaaatc cagtagcctt
     1021 tgctatcaaa gcctaaaatc ggcacaatcg cactgcacaa cagcaccact atcgacaacc
     1081 gaacggcgtg tcgaaacagt tgggattcaa aggttaaatg ggctttaatt gcttggctga
     1141 tattgcttaa gccggtaata ttttcggcga tcaagcggtg agattttgtg aaatttttac
     1201 ttaacggttc aatatgctca ccactgcttg agccttgttc aatctgggct agttgccgct
     1261 caatattgcc taaattttct gcaatagcac gccagcgata agcacttttc aagccccgtt
     1321 cttgatgata attcaatgaa ttaagcaagc cctgcaaggc tttttcgcca cgaacgccgt
     1381 gttcatatac ttctctatgg cgtaacgaca ttgcaatttt ttgacacgcc gcagcctgta
     1441 attccagcac ccgttgaaag cggaacatta aatcactgtt acttaattgt tggaacagtt
     1501 cgtgatattg ataatggctg gaacttgccc gctctaaaat atcttgagcg gtgaaatagt
     1561 aacgcaacat tcgctgtgtc cgttgctgct tgtgctggcg gcttaggcgg tagaataggg
     1621 caactctggt tttatcaaag gcagccatca cctcactatt ggctttcgcc aaggctagtt
     1681 gtttggcagc taaatcgtca tcatcagggt caaaataggc agatttcgcc agtaaataat
     1741 ggctcaacgc aaggtaggcg gacgctaaat tctcctgcac ggttcgattc gggaaaatca
     1801 ggtacaccaa aataccgacc aaaccataga gtaaggtgcc aagtaagatc atcaccacat
     1861 tgtgatacca caatacttcg ggcgtataag ccaggctgtt ataaaccgcc acaatcagcg
     1921 tgccaaaagc aatggtacta tagcgttgcc cgattgcccc taacattacc agcaaaaagg
     1981 tgcaaaccac cgccaccggc acaaacagcc agcccaaatg gagcgacaca ctcgccccaa
     2041 tcgaggaaag cgagaacgcc actaggctga taatcagatt tttcgctcgt ccgattaggc
     2101 tgttgtctaa atccaccaaa ccgccggcga taatgcctaa catcaacggc atcgcaaaat
     2161 cagaaatacc gagctgccaa atgctgaccg ccgcaatgtt tagcgaaata aacatcggca
     2221 gggttttaat tacattttcc gaaagccaac tgtgttgtag atatcttaaa aatttattca
     2281 tatttgctca tttcgggcag gcacaggacc tgcccctacc tacactaccc caaacgttct
     2341 gccaaatagc catcataatc cggcacattc acgctgattg cttgattgaa caaatccgca
     2401 tttaacagaa aattagcggt ggtttcgtta atcgccaccg gaatattcca aacggtcgca
     2461 atccgcatca gggctttcac atcaggatcg tgcggagcgg cattcatcgg atcccaaaag
     2521 aacaccagca gatcaatttt cttttctgcg atcagcccgc ctaattgctg atcgcccccc
     2581 atcgggccgc ttaacaatga agtaatttca agccctgttt ctctggcgag caaatgcccg
     2641 gtagtgccgg tggcatatag cttatgcgag gcaagttggg cttgatgagt ttgtgtccaa
     2701 cgaattaaat cttgtttaca gctatcgtga gccactaagg caatctgttt ttgttttgcg
     2761 atggttctga cggtggtttt catttttagc tcctttgcaa aatttttcac aaatcatacc
     2821 gcttattttt cgcttttgca ttgactaatc acggcttatg gctaaaatca ggtaaaaatt
     2881 cgcattttaa aacaaaggat taaccgcaat gaacttatac aacatcaaac accctgaaga
     2941 acaagtcaat ttcgcccaag cggtacgcca aggcttaggc aaaaatcaag ggctattttt
     3001 ccctgaagtg ttacctaaat tagatagcat tgatgaatta ttggatttac ctttggttaa
     3061 acgcagccaa aaaattcttt cagccttaat cggcgaagag ttaccgcaag aaaccttaaa
     3121 tgcaatggta aaaaatgcgt tcactttccc ggcaccgctt gccaaagtca atgatgatat
     3181 ttatgcgtta gaattatttc acggtccgac tttagccttc aaagattttg gtgggcgctt
     3241 tatggctcaa gccttagcca atgtacgagg agacggcaaa attactattt taaccgctac
     3301 ctcaggtgac accggtgcgg cggtggctca tgctttctat ggtttggaaa atatcaacgt
     3361 ggtcattctt tacccgaaag gcaaaatcag cccattacaa gaaaaattat tctgtacctt
     3421 aggcggtaat attcgcactg ttgcgattga atccgacttt gatgcttgcc aagccttagt
     3481 gaaacaagcc tttgatgatg ccgagctacg ccaagcgatt ggtttaaatt ccgcaaattc
     3541 catcaacatt agccgtttac tggctcaaat ttgctactac tttgaagcag ttgctcagtt
     3601 accaaaagaa aaacgagata acgtagtggt ttcagtacca agcggtaatt tcggtaactt
     3661 aaccgccggc ttaattgcca aaacattagg tttaccgatt aaacgtttta ttgcctcaac
     3721 caatgccaat gacaccgtgc cacgctattt acaatcaggc aaatgggaac caaacgcaac
     3781 cgttgcaacc ctttctaatg caatggacgt aagccgcccg aacaactggc cacgagttga
     3841 agaattattt aaacgcaatg gctggagttt gtctgaattg ggttctgccg cattaaacga
     3901 tgccgaaacc gaagcggctt tgaaagctca atatgcagaa ggttatctgt gtgagcctca
     3961 cggtgctatt gcttaccaaa gcttgaaaga tcagctacaa caaggcgaaa ccggtatttt
     4021 cttatgcacc gctcacccgg caaaattcaa agagtcggtg gaacacattt tagcgattga
     4081 attgccgtta ccggaagcat tagataagca caacaaactg ccactgttat ccgatgaaat
     4141 ggcagcagat tttgcaacat tgcgtgcata tttgttgaaa taaatat
//
LOCUS       DANLZC010000170         4165 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.170, whole genome shotgun sequence.
ACCESSION   DANLZC010000170 DANLZC010000000
VERSION     DANLZC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.170"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            59..2068
                     /locus_tag="PXE44_002315"
     CDS             59..2068
                     /locus_tag="PXE44_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDL5589776.1"
                     /translation="MDLIKKLNTGSTFRVPIFLPSLLFVSFVAVFCIIFPQQAQTSLD
                     TIKNSLFQHFSWFYIFAGSIFFLFLIFLSFSRLGDIKLGADTDEPEFGFGSWIAMLFA
                     AGMGIGLMYFGVAEPILHYLKPVQQNLTEPERMKEAMMTTFYHWGIHAWAIYGVIALA
                     LAYFGFRYKLALTIRSGFYPLLKHRISGFWGHLIDIIALCSTIFGLTTTLGFGVMQVS
                     AGFNNLGLIEQSNFTVLAIIVTVAMALAVLSAVSGVGKGVKILSEINLTLAGLLLIFV
                     IITGPTLLLFSSFTENLGYYFSSLLEMSFRTFAYEPEHQGWLSGWTVLYWAWWASWAP
                     FVGLFIAKISKGRTIREFILGVLFVPSLFNILWMTSFGSSAIWFDQQTAGALAEVSGN
                     TEQLLFTFFEQLPFGSIASFVAVIVISIFFITSADSGIFVLNSIASQGEENAPKWQSV
                     LWGALLAILALSLLYSGGLASLQTMTLIIALPFTFIMLILCIGLWKGLMVDNQYFNKK
                     FSQGSQHWAGKDWKQRLEKIINPSNKQDVRHFFIKVARPAFLELIEEFESYGLIAKMN
                     FTNEQNPKLEFEVVKENLRNFIYGIESVPRELSDLVVGDDNLPNIEQNTIYEPITYFL
                     DGRKGYDVQYMTKEELIADVLQQYERFINLAMDNSHDLMTADFNH"
     gene            complement(2133..2600)
                     /locus_tag="PXE44_002316"
     CDS             complement(2133..2600)
                     /locus_tag="PXE44_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HDL5589777.1"
                     /translation="MKSLKLKIPPPAWFLLCAALMWLVAQYVPVSLPNYQHTAIFFSG
                     LAVAIIGGAVAVSALWVIHQAKTTYSPFRPQHTAKLVTWGIYRKSRNPMYLSLLLGLI
                     AWALWLGAMLAWLVLPLFVWLITYFQIKPEEQILAQKFGTHYAKYCNQVRRWL"
     gene            complement(2602..4149)
                     /gene="murJ"
                     /locus_tag="PXE44_002317"
     CDS             complement(2602..4149)
                     /gene="murJ"
                     /locus_tag="PXE44_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDL5589778.1"
                     /translation="MIVSFMTLISRILGLIRDIVVATILGTGVSADIFLFANRIPNFL
                     RRLFAEGAFSKAFVPVLAEYNADNDPNKTREFIAKVSGTLGGLVTVVTLVAMIASPVV
                     AALFGTGWFLDWLYDGPNAEKFTQASFLLKITFPYLWFITFVALSGAVLNTIGKFGVM
                     AFSPVLLNVAIIAVALWGRDYFDSPDIALAWGVFLGGLLQFLFQIPFMKKEGLLVKPK
                     WAWKDEGVTKVRKLMIPALFGVSVTQLNLLLNQVIASFLITGSISWMYYADRLIEFPL
                     GLFGIAISTVVLPSLSRIAKNKELTEIQRGEHFQNTMDWGVRMVLLLGIPAMIGMAVL
                     AQPIIMTMFMRGKFGFEDVLATSYPLWVMCLGLNSYMLISVLANGFYANQNTKTPVKV
                     GIIAALSNICFGLAFAPFLGYIGLALASACSALVNVSLLYVNLSKNGYYKVSHKTVLF
                     VLKLFIAACVMGALVAYFTPEIELWALMTMWQKVYWLIWLMILAGGSYFASILVLGIR
                     KKDFRSA"
BASE COUNT         1224 a          932 c          835 g         1174 t
ORIGIN      
        1 ttttatatct ttttgctata ttcaagcctc taaccattca aaaatagagg aaatagcatt
       61 ggatttaatc aaaaaattaa acacaggaag tacctttagg gtaccgattt tcctaccgag
      121 tttactcttt gtcagctttg ttgccgtttt ctgtatcatc tttccacagc aagcacaaac
      181 ctcacttgat accatcaaaa atagtctctt ccaacatttt agctggttct atatttttgc
      241 aggctctatc tttttcctgt ttctaatttt tctctctttc agccgattgg gtgatattaa
      301 attaggggca gataccgatg agcctgaatt tggttttggc tcttggattg cgatgttatt
      361 cgccgccgga atggggatcg ggttaatgta ttttggggta gcagaaccta ttttgcatta
      421 ccttaaaccc gtccaacaaa atttaactga gccggagcgt atgaaagaag cgatgatgac
      481 aacgttctat cattggggta ttcacgcttg ggcaatttat ggtgtgattg ccttagctct
      541 tgcttatttt ggcttcagat ataagttagc actcactatt cgttccggat tttatccctt
      601 actaaaacat cgtatttcag gcttctgggg gcatttaatt gatattattg ccctttgtag
      661 cacgattttc ggtttaacga ctacacttgg ctttggggtg atgcaggtca gtgctggctt
      721 taacaatcta ggtttaattg aacagagcaa ttttactgtt cttgcgatta tcgtaacagt
      781 agcaatggct cttgccgtgt tatctgccgt ttcgggcgta ggcaaagggg ttaaaatctt
      841 aagtgaaatc aatctcacat tagccggatt gctacttatt tttgtgataa tcaccggccc
      901 aactctatta cttttctcaa gcttcaccga aaatttaggc tattatttta gctcgctgct
      961 tgagatgagt ttccgtacct tcgcttatga accggaacat caaggctggc taagcggctg
     1021 gacggtcctt tattgggcat ggtgggcatc ttgggcgcca tttgttggtt tgtttattgc
     1081 caagatctct aaaggcagaa ccattcgtga atttatttta ggggtgctat ttgttccatc
     1141 gctgtttaac attttatgga tgaccagctt cggcagctct gccatttggt tcgatcaaca
     1201 aactgccggt gctttagctg aagtcagcgg caataccgaa caactgttat ttaccttttt
     1261 tgagcaatta ccgtttggct ctattgcctc tttcgttgcc gtcattgtta tcagtatttt
     1321 ctttatcacc tctgccgact cggggatttt tgttctcaac agcattgctt cacaaggcga
     1381 agaaaatgca ccgaaatggc aaagcgtgct ttggggagca ttattagcca tcttagcgtt
     1441 atcactactc tattcgggtg gcttggcttc tctgcaaaca atgacactga ttatcgcctt
     1501 accatttacc ttcattatgc tgattctctg tatcggctta tggaaaggat taatggtaga
     1561 taaccaatac ttcaacaaaa aattctcgca aggtagccaa cattgggcgg gtaaagattg
     1621 gaaacaacgc ttggagaaaa tcatcaaccc aagcaataag caagatgtcc gtcacttctt
     1681 tattaaagtt gccagaccag catttttaga acttatcgag gaatttgaaa gctatggctt
     1741 aatcgctaaa atgaatttca ccaacgaaca aaacccgaaa ttagagtttg aagtggtgaa
     1801 agaaaattta cgcaatttca tttacggcat tgaaagtgtg ccacgggaat tatcggattt
     1861 ggtggtaggt gacgacaacc taccgaacat tgagcaaaat accatttacg agccgattac
     1921 ttatttctta gacgggcgga aaggttatga tgtgcaatat atgaccaaag aagagttgat
     1981 tgccgacgtg ctgcaacagt atgaacgctt tatcaattta gcgatggaca actcgcacga
     2041 cttaatgacg gctgatttca atcactaacc ccacaagcgg tgagattttc ggaaaaattt
     2101 gcaaatttcc aacaaaatct caccgcttgc atttaaagcc aacgccttac ttggttgcaa
     2161 tatttggcat aatgtgtgcc gaatttttgg gctaaaattt gctcttccgg cttgatttgg
     2221 aaataggtta tcaaccatac aaacagcggt aaaaccagcc acgccaacat tgcccccaac
     2281 cataatgccc acgcaatcaa acctaatagt aacgaaagat acattgggtt tcggcttttg
     2341 cggtaaatgc cccaagtgac caatttagcg gtatgttgtg gtcgaaaagg actgtatgtc
     2401 gttttcgctt ggtgtatgac ccaaagtgcc gagacggcaa ccgctccgcc aattatcgcc
     2461 acagctaaac cgctaaaaaa tatggctgta tgctgataat tcggcaagct caccggcaca
     2521 tattgagcta ccagccacat taaagcggca caaagcaaga accaagccgg cggcgggatt
     2581 ttcagtttta atgatttcat tttatgccga gcggaaatct tttttacgaa tgcctaatac
     2641 caaaatagag gcaaaataac taccgccggc taagatcatc agccaaatca gccaatacac
     2701 tttctgccac attgtcatta atgcccaaag ttcaatctca ggcgtgaaat aggcaaccaa
     2761 cgcccccatt acacaagcgg cgatgaatag tttcaacaca aaaagcacgg ttttatggct
     2821 tactttatag taaccatttt ttgagagatt cacatacagc aagctcacat tcactaatgc
     2881 cgaacacgca gaagctaacg ctaaaccaat gtatcccaaa aacggagcaa acgccaagcc
     2941 aaagcaaata ttacttaagg cggcaataat cccaactttt accggtgttt tggtgttttg
     3001 gttggcataa aaaccgtttg ccagcacgct aatcaacata tagctgttta accccaagca
     3061 cattacccaa agtggataag aggttgccag cacatcttca aaaccaaatt taccccgcat
     3121 aaacattgtc ataataatcg gctgagccag cactgccata ccgatcattg ccggaatgcc
     3181 gagcaacagc accattctca ccccccaatc cattgtgttt tgaaagtgtt ctccacgttg
     3241 gatttcagtg agttctttgt ttttcgcaat acgggaaagg cttggcagca cgacggtgga
     3301 aatcgcaatt ccaaataaac cgagcggaaa ttcaattaag cggtcggcat aatacatcca
     3361 actaatcgag ccggtaatca aaaacgaggc aatcacttgg tttagcagta aatttagctg
     3421 ggtaacggaa acgccaaata aagccggaat cattaattta cggactttag tgacaccttc
     3481 gtctttccac gcccattttg gcttaaccag taagccctct tttttcataa atggaatttg
     3541 gaataaaaat tgcaataagc cgcctaaaaa taccccccac gctaaggcaa tatccgggga
     3601 atcgaaataa tctctgcccc ataacgctac cgcgataatc gccacattca gcaacaccgg
     3661 ggaaaatgcc atcaccccaa acttaccgat ggtatttaac accgcaccgg ataacgccac
     3721 aaaggtaata aaccacaaat aagggaaagt gattttgagc aaaaaagagg cttgggtgaa
     3781 tttttcggca tttggcccgt cgtaaagcca atctaaaaac caacctgtac caaaaagagc
     3841 ggcaaccaca ggcgaggcaa tcattgccac aagggtaaca acagttacca aacctcctaa
     3901 tgtacccgat actttggcaa taaattctct cgttttgttt ggatcgttat ccgcattata
     3961 ttccgccaac accggcacaa aggctttaga aaatgcccct tcggcaaaca aacggcgaag
     4021 aaaattggga atacggttgg caaacaggaa aatatcggca ctcacgcctg tgcctaaaat
     4081 ggttgccacc acaatgtctc gaatcaaacc gagtatgcga gaaattaagg tcataaagct
     4141 tacgatcatt cctgatctta aaagt
//
LOCUS       DANLZC010000171         4127 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.171, whole genome shotgun sequence.
ACCESSION   DANLZC010000171 DANLZC010000000
VERSION     DANLZC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.171"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(230..1870)
                     /gene="pgi"
                     /locus_tag="PXE44_002318"
     CDS             complement(230..1870)
                     /gene="pgi"
                     /locus_tag="PXE44_002318"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL5589779.1"
                     /translation="MQKINPVQTFAWNALEQHKADNLSIPQLFAEDPNRFDKYSLRFE
                     DQLLVDFSKNAINEKTLTLLRQLANECGLESAKNAMFSGKKINRTENRAVLHVALRNR
                     ANTPIAVDGKDVMPEVNAVLAKMKDFCERVISGEWKGYTGKTITDVINIGIGGSDLGP
                     YMVTEALRPYKNHLNMHFVSNVDGTHIAEVLRKVNPETTLVLVASKTFTTQETMTNAL
                     SARKWLLDSAKDESAVAKHFVALSTNAKEVEKFGIDTANMFEFWDWVGGRYSLWSAIG
                     LSIALSLGFENFEQLLAGAHAMDKHFLITPIEKNIPTTLALVGIWNNNFLGAESEAIL
                     PYDQYMHRFAAYFQQGNMESNGKFVGRDGKPVTHQTGPIIWGEPGTNGQHAFYQLIHQ
                     GTKLIPCDFIAPAQTHNPLSDHHNKLLSNFFAQTEALAFGKSKETVEKEFLDAGKTLE
                     EVAEIVPFKVFSGNKPTNSILVQKITPYSLGALIAMYEHKIFVQGVLFNIYSFDQWGV
                     ELGKQLANRILPELETDEKINSHDSSTNGLINQFKAWR"
     gene            complement(1903..2151)
                     /locus_tag="PXE44_002319"
     CDS             complement(1903..2151)
                     /locus_tag="PXE44_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HDL5589780.1"
                     /translation="MLTPKNLEAIAQQLHNALPQSLKNVGNDLDEKFKQILQAQLARL
                     DVVTREEFDVQSQVLLRTREKLTELEKRLDELTQRTDE"
     regulatory      2275..2319
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2323..2991
                     /gene="queC"
                     /locus_tag="PXE44_002320"
     CDS             2323..2991
                     /gene="queC"
                     /locus_tag="PXE44_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDL5589781.1"
                     /translation="MTHQKPKAVVIFSGGQDSTTCLFQAIAEFGKENVEVVTFQYGQR
                     HSIELEKARWISNDLGVKQTLIDTSVIKAITTNALMDNNAEIQQQGSTPNTFVDGRNA
                     LFLLYTAIYAKGQGIQTIFTGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYNFNIRTP
                     LMYLTKKETWALADKLGAFDYIRDHTHTCYLGVEGGCHDCPSCVLREKGLNEYLAERE
                     VSNV"
     gene            2984..3406
                     /gene="queD"
                     /locus_tag="PXE44_002321"
     CDS             2984..3406
                     /gene="queD"
                     /locus_tag="PXE44_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDL5589782.1"
                     /translation="MFKIAKEFSFDMAHMLDGHDGKCQNLHGHTYKLQVEVAGELFQE
                     GAKRGMVMDYSDLKAVVKQHILEPMDHAFIYDLQSERESQVAQLLIDLNSKVYGIPTR
                     TTAEEMAKYMFEKLEKVGLPVSLIRLWETPTSYCEYSK"
     gene            3406..4032
                     /locus_tag="PXE44_002322"
     CDS             3406..4032
                     /locus_tag="PXE44_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDL5589783.1"
                     /translation="MAELISNPRFPIVEIFESLQGEGFNTGMPAIFVRFGKCNLTCPW
                     CDTNYNQFEQWTLSDILAKVRSYSAKNVIITGGEPTIQPNLSLLLEQFKKEGYFLAIE
                     TNGLKEVPAQIDYIATSPKRMYQEKYQRRCIEFANEVRIVVDGEVQGFCEQIESQIKA
                     EHYYLSPCEVEGKMNLLETITQIGLLNQRPNKPKWQLSIQTHKIVGIE"
BASE COUNT         1253 a          757 c          875 g         1242 t
ORIGIN      
        1 tgagtgaaga gtagaggtaa aatagtaagc ctttttaacc agtttattta ttgtacctat
       61 tttagaaaaa agaaaagtag aaaagagata ttaggtaaac tcatagtgca aaagaatata
      121 gaaaaaactg cttgtgtaga aatggcgctt agttcagtcc tcagctaaaa ttttagaaaa
      181 aaagcataga gattttttac ctctatgctt tttattgtat tgactatatt taacgccaag
      241 ccttgaattg gttaattaaa ccatttgtgg agctgtcgtg gctgttgatt ttttcatcgg
      301 tttcgagttc gggtaaaatg cggtttgcaa gctgtttgcc taactccacg ccccattggt
      361 cgaaactgta aatgttgaac agcacgcctt gcacgaagat tttgtgttca tacattgcaa
      421 ttaatgcccc taagctgtac ggggtgattt tttgcaccaa gatagagttg gtcggtttgt
      481 ttccgctgaa cactttaaac ggcacgattt cagctacttc ttccaacgtt ttacctgcat
      541 ctaaaaactc tttttcaact gtttcttttg atttaccgaa agctagtgct tcagtttgag
      601 caaagaagtt agaaagcagt ttgttgtgat gatcgcttaa cggattgtga gtttgtgccg
      661 gtgcgataaa atcacaagga attaattttg tgccttgatg aatgagttga tagaatgcgt
      721 gctgaccgtt agttcccggt tcgccccaaa taatcggacc tgtttggtga gtaaccggtt
      781 tgccgtcacg ccccacaaat ttaccgtttg attccatatt gccttgttgg aaataagccg
      841 caaagcggtg catatattgg tcgtaaggta aaatcgcttc tgattcagca cctaagaaat
      901 tgttattcca aatgccgact aaagctaacg tagttggaat gtttttctca atcggcgtga
      961 ttaagaaatg cttatccatt gcgtgagcac cagcaagtag ctgctcgaag ttctcaaaac
     1021 ctaatgaaag ggcaatcgaa agcccgatgg cagaccataa tgagtaacga ccgccaaccc
     1081 aatcccagaa ttcaaacata ttagcggtat caataccgaa tttttccact tctttggcat
     1141 tggttgaaag tgccacgaaa tgttttgcta ccgcagattc atctttggca gaatcaagta
     1201 gccatttgcg agctgaaagg gcattggtca tcgtttcttg agtggtaaag gtttttgaag
     1261 ccactaacac taacgtggtt tcaggattaa ctttacgcaa cacttcagca atatgcgtgc
     1321 cgtccacgtt agacacaaag tgcatattaa ggtgattttt ataagggcgt aatgcctctg
     1381 ttaccatata aggacctaga tccgagccac caataccgat attgatcaca tcagtaatcg
     1441 ttttgccggt atagcctttc cattcgccgg aaataacacg ctcgcagaaa tctttcattt
     1501 tcgctaacac ggcattcact tccggcatta catctttgcc atcgactgcg attggtgtat
     1561 tggcacggtt acgcagtgca acgtgcaata cggcacggtt ttcagtgcgg ttgatttttt
     1621 tgccggagaa catcgcattt tttgctgatt ccaaaccaca ttcatttgcc agttggcgaa
     1681 gtaaggttaa ggttttttcg ttgatcgcat tttttgaaaa atcgactaat aattgatctt
     1741 caaaacggag ggaatatttg tcgaaacggt ttggatcttc ggcaaaaagt tgaggaatag
     1801 aaaggttgtc tgctttatgt tgctctaagg cattccaagc aaaagtttgc acagggttaa
     1861 ttttttgcat ctgagtttcc ttatactaaa ttaaaaaatc gattattcat ctgttctttg
     1921 agtcagttca tcaagacgtt tttctaattc ggttaatttt tcacgagtgc ggagtaacac
     1981 ttgagattga acatcaaatt cttcacgagt gacaacatca agacgggcta attgggcttg
     2041 aaggatttgc ttaaattttt catctagatc attaccgaca ttttttaagc tttgcggtaa
     2101 ggcgttatgg agttgttgag caatcgcttc taagttcttc ggcgtgagca tatttaaatt
     2161 ccttactata aatgagtatc ccaattttat tgaattttgg gtttaagaga aagcaaattc
     2221 tttgtaaaat atcacctttc gcaaaataca agcaaaaaag accgcttgta ttttttattt
     2281 gtggttcgta aacctcccac acaaaaaaac taaggacaca aaatgactca tcaaaaacca
     2341 aaagccgttg tgattttttc cggcggtcaa gactctacca cttgtttatt ccaagctatt
     2401 gctgaatttg gcaaagaaaa tgtggaagtc gttacctttc aatatgggca acgccattcg
     2461 attgaacttg aaaaagcacg ctggatttct aacgatttag gcgtcaaaca aacgctgatt
     2521 gatacttcgg tgattaaagc gattactacc aatgcgttaa tggataataa tgctgaaatt
     2581 caacaacaag gcagcacacc caataccttt gtggatgggc gtaatgcctt atttctgctt
     2641 tataccgcca tttatgccaa agggcagggc attcaaacta tttttaccgg cgtgtgtgag
     2701 accgatttta gtggttaccc tgattgccga gatgtatttg taaaatcaat gaatgtaacg
     2761 ctaaatttag cgatggacta taatttcaac attcgcacgc cattgatgta tttaactaaa
     2821 aaagaaactt gggcattagc agataagctg ggtgcttttg attatattcg agaccatacc
     2881 cacacctgtt atttaggcgt tgaaggtggt tgccacgact gcccgagttg cgtattgcga
     2941 gaaaaaggct tgaatgaata tttagcagaa agggaggtaa gcaatgttta aaattgctaa
     3001 agaatttagt tttgatatgg ctcatatgct agatggacac gatggcaaat gccaaaactt
     3061 acacggacac acctacaaat tgcaagttga ggtggcaggt gagctatttc aagagggagc
     3121 taaaagagga atggtaatgg actattcgga tctaaaagca gtggtaaaac aacatatttt
     3181 agagccgatg gatcacgctt ttatttacga cttacagagt gagcgtgaaa gccaagtagc
     3241 acaattatta attgatttaa actccaaggt ttatggtatc ccaactcgta ccacagccga
     3301 agaaatggcg aaatatatgt ttgaaaaatt agagaaagtg ggcttaccgg ttagcctaat
     3361 tcgcttgtgg gaaacgccta cttcttattg tgagtacagc aaataatggc ggagttaatt
     3421 tcaaaccctc gttttccgat tgttgagatc tttgaaagct tacaagggga aggctttaat
     3481 accggaatgc cggctatttt tgtacgtttt ggcaaatgca acctcacttg cccgtggtgc
     3541 gataccaatt acaatcaatt tgagcaatgg acgttaagcg atattcttgc caaagtaaga
     3601 agctattctg cgaaaaatgt gattattacc ggaggcgagc caacgattca gcctaattta
     3661 tcgttattat tagaacaatt taaaaaagag ggatattttt tagcgatcga aaccaacggc
     3721 ttaaaagagg ttccggcaca aattgattat attgcgacca gcccaaagcg aatgtaccaa
     3781 gaaaagtatc aacgccgttg cattgaattt gcgaatgaag tccgtatcgt cgttgatgga
     3841 gaagtacagg gtttctgtga gcaaatagaa agccaaatta aagctgagca ttactatctt
     3901 tcaccttgtg aggtagaggg aaaaatgaac ttgctggaaa ccatcaccca gattgggtta
     3961 ttaaatcagc gtccaaataa gcctaaatgg cagctaagta tccaaacgca taaaattgtg
     4021 gggattgaat aagtagaagt tagaagatga tttgtaaaag atcagacttg atctgacaat
     4081 tcactctaaa aagtgagagt ttcccgttta gaatatgagt gtccaaa
//
LOCUS       DANLZC010000172         4123 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.172, whole genome shotgun sequence.
ACCESSION   DANLZC010000172 DANLZC010000000
VERSION     DANLZC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.172"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(77..781)
                     /locus_tag="PXE44_002323"
     CDS             complement(77..781)
                     /locus_tag="PXE44_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDL5589784.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            complement(872..1699)
                     /locus_tag="PXE44_002324"
     CDS             complement(872..1699)
                     /locus_tag="PXE44_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_034033256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-2"
                     /protein_id="HDL5589785.1"
                     /translation="MAIRIFAILFSIFSLATFAHAQEGTLERSDWRKFFSEFQAKGTI
                     VVADERQADRAMLVFDPVRSKKRYSPASTFKIPHTLFALDAGAVRDEFQIFRWDGVNR
                     GFAGHNQDQDLRSAMRNSTVWVYELFAKEIGDDKARRYLKKIDYGNADPSTSNGDYWI
                     EGSLAISAQEQIAFLRKLYRNELPFRVEHQRLVKDLMIVEAGRNWILRAKTGWEGRMG
                     WWVGWVEWPTGSVFFALNIDTPNRMDDLFKREAIVRAILRSIEALPPNPAVNSDAAR"
     gene            complement(1776..2567)
                     /locus_tag="PXE44_002325"
     CDS             complement(1776..2567)
                     /locus_tag="PXE44_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA25"
                     /protein_id="HDL5589786.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2625..3158)
                     /gene="aadB"
                     /locus_tag="PXE44_002326"
     CDS             complement(2625..3158)
                     /gene="aadB"
                     /locus_tag="PXE44_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="HDL5589787.1"
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDD
                     IDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEA
                     PQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLG
                     AEKVEVLRAAFRSRYAA"
     gene            3345..3968
                     /gene="tetR(H)"
                     /locus_tag="PXE44_002327"
     CDS             3345..3968
                     /gene="tetR(H)"
                     /locus_tag="PXE44_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(H)"
                     /protein_id="HDL5589788.1"
                     /translation="MAKLDKEQVIDNALILLNEVGIEGLTTRKLAQKIGVEQPTLYWH
                     VKNKRALLDALAETILQKHHHHVLPLPNETWQDFLRNNAKSFRQALLMYRDGGKIHAG
                     TRPSESQFETSEQQLQFLCDAGFSLSQAVYALSSIAHFTLGSVLETQEHQESQKEREK
                     VETDTVAYPPLLTQAVAIMDSDNGDAAFLFVLDVMISGLETVLKSAK"
     gene            complement(3971..4108)
                     /locus_tag="PXE44_002328"
     CDS             complement(3971..4108)
                     /locus_tag="PXE44_002328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589789.1"
                     /translation="MPENGVIFSDGIEDDYLQFNAGCIAHIGIADIQGQLISQKGRQR
                     I"
BASE COUNT         1027 a         1067 c          979 g         1050 t
ORIGIN      
        1 agtgccgatt gaccgagctt taacggcact gttgcaaagt tagcgatgag gcagcctttt
       61 gtcttattca aaggccttac atttcaaaaa ctctgcttac caggcgcatt tcgcccaggg
      121 gatcaccata ataaaatgct gaggcctggc ctttgcgtag tgcacgcatc acctcaatac
      181 ctttgatggt ggcgtaagcc gtcttcatgg atttaaatcc cagcgtggcg ttgattatcc
      241 gtttcagttt gccatgatcg cattcaatca cgttgttccg gtacttaatc tgtcggtgtt
      301 caacgtcaga cgggcaccgg ccttcgcgtt tgagcagagc aagcgcgcga ccataggcgg
      361 gcgctttatc cgtgttgatg aatcgtggga tctgccactt cttcacgttg ttgaggattt
      421 tacccagaaa ccggtatgca gctttgctgt tacgacggga ggagagataa aaatcgacag
      481 tgcggccccg gctgtcgacg gcccggtaca gatacgccca gcggccattg accttcacgt
      541 aggtttcatc catgtgccac gggcaaagat cggaagggtt acgccagtac cagcgcagcc
      601 gtttttccat ttcaggcgca taacgctgaa cccagcggta aatcgtggag tgatcgacat
      661 tcactccgcg ttcagccagc atctcctgca gctcacggta actgatgccg tatttgcagt
      721 accagcgtac ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca
      781 tgtgcagctc catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag
      841 tgcccgttga agtaaccggc gctgcgcggt tttatcgcgc agcgtccgag ttgactgccg
      901 ggttgggcgg taacgcttca atagagcgaa ggattgcccg cacgattgcc tccctcttga
      961 aaagatcatc cattctgttt ggcgtatcaa tattcagtgc gaagaatacg gagccagtcg
     1021 gccactcaac ccatcctacc caccaaccca tacggccttc ccagcccgtc tttgcacgca
     1081 gtatccagtt gcgaccggct tccacaatca tgagatcctt gaccaagcgc tgatgttcta
     1141 cccgaaaggg cagctcgtta cgatagagct tcctgagaaa tgcaatttgc tcctgcgccg
     1201 agattgcaag gctgccttct atccagtaat cgccattact tgtcgaagga tcggcgttgc
     1261 catagtcgat tttcttcaaa tagcgccgag ctttgtcatc accaatttcc tttgcaaata
     1321 gctcatacac ccaaacagta gaattccgca ttgctgatcg caaatcttgg tcttgattgt
     1381 ggcctgcaaa gcccctgtta acgccgtccc atcgaaaaat ctggaactca tcacgaacag
     1441 cgcctgcatc aagtgcaaaa agtgtatgag gtatcttgaa tgtcgatgca ggcgagtagc
     1501 gtttcttcga tcgcacagga tcaaaaacca acatggcacg atccgcttgg cgttcgtctg
     1561 ccacaactat cgtgcctttg gcttgaaatt cgctgaaaaa cttcctccag tcagaacgtt
     1621 ctagcgtgcc ttcttgcgca tgcgcgaaag tggcaagaga aaaaatggag aaaagtatcg
     1681 cgaagattcg gattgccatt aacttttcct taatgcccaa cggccaaggt aagccgcgcc
     1741 gcgaagcggc gtcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc
     1801 tcgcctttca cgtagtgaac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
     1861 tcttcttgtc caagataagc ctgtctagct tcaagtaaga cgggctgata ctgggcaggt
     1921 aggcgtttta ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttattgcg
     1981 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
     2041 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
     2101 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
     2161 gtcagcaaga tagccagatc aatgtcgatc atggctggct cgaagatacc cgcaagaatg
     2221 tcattgcgct gccattctcc aaattgcagc tcgcgcttag ccggataacg ccacgggatg
     2281 atgtcgtcat gcacgacaag ggtgacttct atagcgcgga gcgtctcgct ctcgccaggg
     2341 aaagccgaag cctccataag atcattgagc aatgctcgcc gcgtcgtttc atcaagcttt
     2401 acggccacag taaccaacaa atcaatatcg ctgtatggct tcaggccgcc atccactgcg
     2461 gagccgtaca aatgcacggc cagcaacgtt gattccagat ggcgctcaat gacgcttagc
     2521 acctctgata gttggttcga aatttcgatg gtcaccgctt ccctcatgat gtctaacacc
     2581 tgagttaagc cgcgccgcga agcggcgtcg gcttggacga attgttaggc cgcatatcgc
     2641 gacctgaaag cggcacgcaa gacctcaacc ttttccgccc cgagtgaggt gcatgcgagc
     2701 ctgtaggact ctatgtgctt tgtaggccag tccactggtg gtacttcatc ggcatagtaa
     2761 aagtaatccc agatgatcgc ctcccagctg ttacaacgga ctggccgccc ggcgatgacg
     2821 ccctcagccg cctctgggca cgagccctgc ggagcctccg cgatttcata cgcttcgtct
     2881 gcccaccaag caggttcgca gtcaagtaac tcatccccga tctccgctaa gaatccatag
     2941 tccaactcct ccatgacgcg cccgccgagc atttcaacta ttgcctcgag ctcgccgcgc
     3001 ctctcgccgg gaaacgtcag atcaatatca tcgtgcttgc gtgttacacg ccctagccgt
     3061 gcatcgatcg cccagccccc accgatccag agcggcagat ttcgctcatc tgccgcagct
     3121 agaattttgt gtatcaatgt gacctgcgtt gtgtccatgc ggcctaacct ttgttttagg
     3181 gcgactgccc tgctgcgtaa catcgttgct gctaatacgg tgatcagcag tataataata
     3241 attgatttat tcattgagat cctaaaaatc tatcagtgat agagtgggtg taatatatct
     3301 tatcagtgat agattgtcaa ggctatttta ttttgaggta ggtaatggca aagctagata
     3361 aagaacaagt tattgataat gcgttgattt tacttaatga agttggtatt gaaggattaa
     3421 caacgcgtaa gctggcgcaa aaaataggtg tggaacaacc cacattgtat tggcatgtaa
     3481 aaaataaacg cgctttgtta gatgcattag cagaaactat tttgcaaaag caccatcatc
     3541 atgttttgcc attgccgaat gaaacatggc aggacttttt gcgaaataac gcgaaaagct
     3601 tccgccaagc cttattaatg tatcgtgatg gtggcaaaat tcatgcggga acacgcccct
     3661 ctgaaagtca atttgagaca tcagaacagc aactacagtt tttgtgtgat gctgggttta
     3721 gtctatctca agccgtgtat gcattaagct ctattgcgca ttttacatta ggctccgtac
     3781 tggaaactca agagcatcaa gaaagccaaa aagagcgtga aaaagtagag acggatactg
     3841 ttgcctatcc gccattatta acccaagccg ttgcaattat ggatagtgat aatggtgatg
     3901 ctgcattttt gtttgtcctt gatgtgatga tctctggact tgaaacagta ttaaagagcg
     3961 ctaaataaat ctaaattcgc tgacgccctt tttggctaat tagttgccct tgtatgtcag
     4021 cgataccaat gtgagcaata caacctgcat taaattgtaa atagtcatct tcgatgccat
     4081 cagagaaaat tacgccattc tctggcatca aagatcctaa ttg
//
LOCUS       DANLZC010000173         4109 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.173, whole genome shotgun sequence.
ACCESSION   DANLZC010000173 DANLZC010000000
VERSION     DANLZC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.173"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..386
                     /locus_tag="PXE44_002329"
     CDS             <1..386
                     /locus_tag="PXE44_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589790.1"
                     /translation="KRISLHLKILDFDKGRNTYLAPADLFILDEHLNEFILGNGGDEI
                     EKISKNNKTQFSSTIRENQINFIRALLYMDYGIETPQEVKNAINSGELGRKIEKFRRE
                     NPNIENDKNFKFPTSVTLSNWYSKA"
     gene            464..715
                     /locus_tag="PXE44_002330"
     CDS             464..715
                     /locus_tag="PXE44_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5589791.1"
                     /translation="MQLQKTISTFTQPNHRGIYVMTYSEQSYLNVKDVCEMLQLNRST
                     IYRQIKQGIFPKPTKIGKSSRWKFADIKAFLEPKPQEAN"
     gene            715..1095
                     /locus_tag="PXE44_002331"
     CDS             715..1095
                     /locus_tag="PXE44_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5589792.1"
                     /translation="MAGLSIICPFCGNTARIRGSSRPSLLSYQAQLTCSNCGQLKADF
                     VGQLINIKRAVFIECDEANQWDKPENELIKEGKIQGKDNAQRLKELRGSQQDLFAKVP
                     GQLPPEKITPAEKIAKRANLSKTH"
     gene            1406..2467
                     /locus_tag="PXE44_002332"
     CDS             1406..2467
                     /locus_tag="PXE44_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDL5589793.1"
                     /translation="MKDLSPNQKQYTPFSAIMQKNLMDLLTKSARSPYSFSTVAKSTA
                     GRENPELHNTANSSTPLTRAIFIRSLHTPKERRNKACSSMVACSGQGSPLAVFGSPFV
                     AVFSPRYTLSPCTVRSVADSPLKLTNGLQKMNPSQITGKNRLNSFLFNNNQNDYSLLF
                     TNAKQSTPISAKISKNQDLCLTTLNRSPYASRAFAKSKAECGNSKEHQANNSTPLTRA
                     FFVRSSRTPKECQNKACSSMVACSGQGFALDCLPDVAVFHPVTRYRPTLWKMLAIAPK
                     NQHQELSKMKAFAYSFLCAIRTPETDTYQETVVRIISDSEQNARFQLTADYRLLLPRP
                     VAKIRLNSTAYTVKGGIYA"
     gene            2460..2624
                     /locus_tag="PXE44_002333"
     CDS             2460..2624
                     /locus_tag="PXE44_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589794.1"
                     /translation="MLNYSDIEQIRYFMDQAEEALDNNEEFKAVAELNTAQQFILALL
                     MNYEQGGENE"
     gene            2617..3252
                     /locus_tag="PXE44_002334"
     CDS             2617..3252
                     /locus_tag="PXE44_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589795.1"
                     /translation="MNNTTGQATQRNWQEIIANLQRLSESGESTIEIAKRYEAFSKTA
                     EQIQALVNNNGSEAEKEAFFAPVERFYLGLLETETRLKDAIAKDEENAQKLKEQKAAR
                     EAEQKRWDNLPFYAKDKFSDLICIESRETLEDKFFAISCMFDALKAVPNLLEIGLREE
                     GKDSVIDRLAEMSKLYLLFVDEQFSLLANEIQSGKYGKLDLNGSQNKGDLC"
     gene            3246..3464
                     /locus_tag="PXE44_002335"
     CDS             3246..3464
                     /locus_tag="PXE44_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589796.1"
                     /translation="MLTHRITLPDGTEIEFFAETDKETFAEIQRDLEGLKLPTFAEFV
                     AQERKKHRAKQAVKKARKIAKLARRKNR"
     gene            3606..4091
                     /locus_tag="PXE44_002336"
     CDS             3606..4091
                     /locus_tag="PXE44_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589797.1"
                     /translation="METALQLAQHLTGWTEEENSQAILKNFLDWKETFGEKTREETKI
                     VETLLDWINENEASFIEYPFNQEQRTPPKVAGYRILANNSTKTNEYFFIYPKQFKETL
                     AEFPLLMACTKLHEAGILHKPPRPEKGYEFQYTIAPKITGGSKKRAYKIVPIALEEEN
                     E"
BASE COUNT         1492 a          854 c          788 g          975 t
ORIGIN      
        1 aaaaaagaat aagtttacat ctaaaaatac tggattttga caaaggcaga aatacttatt
       61 tagcacctgc agatttattt attcttgatg aacatctaaa tgagttcata ttaggtaacg
      121 gcggagatga aatagaaaaa atatcaaaga ataacaaaac tcaattcagc tcaacaatac
      181 gagaaaacca aatcaatttt attagagcct tgctttatat ggattatggg atagaaacgc
      241 ctcaagaagt aaaaaacgcc attaattcag gggaactagg cagaaaaata gagaaattta
      301 ggagagaaaa ccccaatata gagaacgata aaaatttcaa attccctaca agtgtaaccc
      361 ttagcaactg gtattcaaaa gcctaaaata ttggaaattt ccgaatctac tttggaaatt
      421 tccaaacttc taaaacctac tcacctcaca atctccccca acaatgcaac tacaaaagac
      481 aataagcaca ttcacgcaac caaaccacag ggggatttat gtaatgacct attcagaaca
      541 aagctattta aacgtgaaag atgtatgcga aatgctacaa ctcaaccgct caactattta
      601 tcgacaaatt aagcaaggga ttttccctaa gccaactaag atcggaaaat caagccggtg
      661 gaaatttgcc gacatcaaag catttttaga gccaaaacca caggaggcga actaatggca
      721 gggctttcta ttatttgccc gttttgtggc aataccgcac gaattagagg cagttctcgc
      781 cctagccttt taagttatca agcacagcta acttgttcaa attgcggaca attaaaagcc
      841 gactttgtag ggcagctaat caacatcaaa cgggccgtat ttattgagtg tgatgaagca
      901 aatcaatggg ataaacccga aaatgaacta atcaaggagg gaaaaattca aggcaaagac
      961 aatgctcaac gcctcaaaga actaagaggc tcacagcaag atttatttgc aaaggtgccg
     1021 gggcaactac cgcccgagaa gatcactccg gcggagaaaa tcgccaagcg ggcaaacctt
     1081 tcaaaaacgc attaaacagg gggataaatg gcaacaaaaa agccccgcaa ggcaaaccat
     1141 acggagctat taaaatcgca taatgcgaaa gatatgcaaa gaaattaaag caggcttgac
     1201 gatttctgaa ggtgttagat tctttctaaa gtctttaggg ataggtttat ttctagcatt
     1261 gtttttttat tttttgccgt atttagcaga atttataatc gccgtaaaaa aacaactagc
     1321 aaaaaaatcg gcttactttc aatgataaaa gcaagccgaa atgacaatcc aaatttttaa
     1381 ctttaaataa agagtacaag aagaaatgaa agatttatct ccaaatcaga aacaatatac
     1441 gccattttcg gctataatgc aaaaaaattt aatggattta ttgacaaaat ccgcccgttc
     1501 gccttattct ttctctaccg ttgcaaaatc aacggcaggg cgtgagaacc ctgaactaca
     1561 taatacggcg aacagtagca cgcctttaac ccgtgctatt tttattcgta gcttacacac
     1621 acctaaagaa cgccgaaata aggcgtgttc gtctatggta gcgtgtagcg ggcaaggttc
     1681 gcccttggct gtttttggtt cgccgtttgt agcagttttc tcaccccgtt acacgctatc
     1741 gccctgcacc gtgagaagtg tagccgatag tcctttaaaa ttaacaaacg gactacaaaa
     1801 aatgaaccct agccaaatta caggcaaaaa ccgcctaaac tcttttctat ttaacaataa
     1861 tcaaaatgat tattcgctat tatttactaa tgcaaaacaa tcaaccccga tttctgctaa
     1921 aataagcaaa aatcaagatt tatgcttgac aaccctcaat cgttcgcctt atgctagccg
     1981 tgcttttgca aaatcaaaag ccgagtgtgg aaactcgaaa gaacatcagg cgaacaatag
     2041 cacgccttta actcgtgctt tttttgttcg tagctcacgc acacccaaag aatgccaaaa
     2101 taaggcgtgt tcgtctatgg tagcgtgtag cgggcaaggt ttcgcccttg actgcttacc
     2161 tgatgttgca gttttccacc ccgttacacg ctatcgccct acattgtgga aaatgttagc
     2221 gatagcccct aaaaatcaac atcaggagct atcaaaaatg aaagcattcg cttattcatt
     2281 cctttgtgct attcgcacac ccgaaacaga tacttatcaa gaaaccgtag tacgcattat
     2341 ttcagacagc gaacaaaacg cccgttttca gcttacagcc gattatcgct tactactccc
     2401 ccgccctgtt gcaaaaatcc gcctaaattc taccgcttac acggtgaaag gggggattta
     2461 tgcttaatta ttccgatatt gaacaaatcc gctattttat ggatcaagcc gaagaggctt
     2521 tagacaataa tgaagagttc aaagccgtag cagaacttaa cacagcacaa caatttattc
     2581 tggctttatt aatgaactac gaacaagggg gtgaaaatga ataatacaac aggacaagct
     2641 acgcaaagaa actggcaaga aattatcgct aatttgcaga gactcagcga atccggggaa
     2701 agcacaatcg aaatcgctaa acgctatgag gcattttcta aaaccgccga gcaaatccaa
     2761 gccctagtga ataacaacgg cagcgaagcc gaaaaagagg cgttttttgc ccctgttgag
     2821 cgtttttatc ttggcttact agaaaccgaa acacgcttaa aagatgccat agccaaagac
     2881 gaagaaaacg cccaaaaatt gaaagagcaa aaagcagcta gagaggcaga gcaaaaaaga
     2941 tgggacaacc tacctttcta cgccaaagac aaattcagtg atttaatttg cattgaaagc
     3001 cgagaaacgc ttgaagataa attctttgcg atctcttgta tgttcgatgc cctaaaagcc
     3061 gtacctaatc tattagaaat aggcttgcgt gaagaaggca aagacagtgt tatagaccga
     3121 ttagccgaaa tgagcaaact ctatttactg tttgtagatg agcaattttc actactggca
     3181 aacgaaattc aaagcggtaa atatggcaag ttagacctaa acggctcaca gaataagggg
     3241 gatttatgct aacgcaccgc attacactcc ctgacggcac agaaattgaa ttttttgcag
     3301 aaaccgacaa agaaacattt gcagaaattc aaagggactt agagggcttg aaattgccaa
     3361 cctttgccga gtttgttgcc caagagcgga aaaaacaccg agcaaaacaa gcggtcaaaa
     3421 aagcccgaaa aattgcaaaa ttagcaagac ggaaaaaccg ctagcaccta tcaaaaagac
     3481 ttattattgc agaaaatcac gctttaataa aaacgaccta taaagcaatc cgcaagaaat
     3541 ggttaagttt aactgaaaat atgagcggac aggttcagcg ggtagcgtgc gatagattcg
     3601 caataatgga aaccgccttg cagttagccc aacatttaac aggctggaca gaggaagaga
     3661 acagccaagc gattttaaaa aacttcctag actggaaaga aactttcggc gaaaaaacaa
     3721 gagaagaaac taaaatagta gaaaccttgc tagattggat aaatgaaaat gaggcgagtt
     3781 ttatcgagta cccattcaac caagagcaaa gaacaccgcc taaagttgcc ggctatagaa
     3841 tacttgcgaa taacagcact aaaacaaatg agtacttttt catttaccct aagcaattta
     3901 aagaaacgct ggcagaattt ccgctattga tggcttgcac caagttacac gaggcaggga
     3961 ttttacataa gccaccacgc ccagaaaaag gctatgaatt tcagtacaca atagccccta
     4021 aaataacagg agggagcaaa aaaagagctt ataaaatcgt accgatagca ttggaagaag
     4081 aaaacgagta attacagcaa aagcaagag
//
LOCUS       DANLZC010000174         4092 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.174, whole genome shotgun sequence.
ACCESSION   DANLZC010000174 DANLZC010000000
VERSION     DANLZC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.174"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(2..979)
                     /locus_tag="PXE44_002337"
     CDS             complement(2..979)
                     /locus_tag="PXE44_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL5589798.1"
                     /translation="MKQIIAQARENAVRAVDFQRVLMYWHIGKRIFEQEQQGQERADY
                     GTYLIKMLAKELEPEFGSGFCSRQLERYRQFYRAFPIATALRSQLNWTQYRRLIQISD
                     SDKREFYLAESCKNNWSSRQLERQINSSLYERLLLSNDKESVLAVARNEQQPVDPKQL
                     IKDPMVLEFLGLKRESAYYEKDLEQAIITHLQDFLLELGNGFSFIARQKRLHLDGDDF
                     FVDLVCYNRLLQCFVIFEIKTHKLTHQDLGQLQMYVNYFDRVEKLPHENATIGVLLCA
                     DKNDAVVKFSLPENQNKIFASQYQLYLPSEQTLIAEVQKEIENFEQKYP"
     gene            complement(1140..1787)
                     /locus_tag="PXE44_002338"
     CDS             complement(1140..1787)
                     /locus_tag="PXE44_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HDL5589799.1"
                     /translation="MLNNVKQQLMLPQFTFGLIETAFNALLKRSPHIEPNLRKLSGKV
                     LKIALTSPKIDFFVLFSESRTDWLGNYEGEADCTVQLAFDTLPKLADKQKLTELINNK
                     SLVLTGDIQVLQHFTALLEQLEKDPAELLSPFVGDVVAQTSTDLAKKLLGKVKAQFEQ
                     NNQHLVENLIQERPVLVHRLQAVHFYDQVEELAQQALKLEQKFTHYVRSSSHQQS"
     gene            complement(1978..2730)
                     /gene="ubiE"
                     /locus_tag="PXE44_002339"
     CDS             complement(1978..2730)
                     /gene="ubiE"
                     /locus_tag="PXE44_002339"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDL5589800.1"
                     /translation="MQQSAETTHFGFKTVAKEEKQQLVANVFHSVAGKYDLMNDLLSF
                     GIHRVWKRFTIDCSGVRKGQKVLDLAGGTGDFTAKFSRIVGESGEVILADINSSMLEV
                     GREKLRNLGVVGNVSYVQANAECLPFADNTFDCIVISFGLRNVTDKDKALRSMFRVLK
                     PGGRLLVLEFSKPIIDPISQLYNFYSFNILPKVGEVVVNDAESYRYLAESIRMHPKQD
                     ELKAMMEQTGFESVNYYNLSAGIVALHRGYKF"
     gene            3075..4028
                     /gene="corA"
                     /locus_tag="PXE44_002340"
     CDS             3075..4028
                     /gene="corA"
                     /locus_tag="PXE44_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDL5589801.1"
                     /translation="MIRAFGLDNACLISVDETNPTLLNDAIWIDLIDPSEAERTVLEN
                     RLDQTLAEERELEDLEASARFFEDEDGLHLHSFFYCLDDDDYADIATVAFTIRDGRLF
                     TLRDRDLPAFRLYRMRSRREKLIDSNAYELLLDLFETKIEQLADVIEEVYADLEKLSR
                     VILNGQQENENFNDALSRLTEFEDASSKVRLCLMDTQRALSFLLRKTRLPNNQLEQAR
                     DIMRDIESLQPHNESLFQKVNFLMQAAMGYINIEQNRIMKFFSVVSVMFLPATLVAST
                     YGMNFEFMPELHFQYGYPMAIGLMIVAASAPYLYFKRKGWL"
BASE COUNT         1131 a          887 c          786 g         1288 t
ORIGIN      
        1 actatgggta tttctgctca aaattctcaa tttctttctg tacttccgca ataagtgttt
       61 gttcagaggg gaggtaaagt tggtattggc tggcaaaaat tttattctga ttttccggta
      121 gcgaaaattt taccaccgca tcatttttat cggcacaaag caagacacca atagtggcat
      181 tttcgtgcgg cagtttttca actcggtcaa aataattgac gtacatctgt aattgcccca
      241 aatcttgatg ggtgagcttg tgggttttaa tttcaaaaat cacaaaacat tgaagtaaac
      301 ggttgtaaca aaccaaatcg acaaaaaaat catcaccgtc taggtgcaaa cgcttttggc
      361 gtgcgataaa cgagaaaccg ttacccaatt ctagtagaaa atcctgcaaa tgcgtaatga
      421 tggcttgctc taaatccttt tcgtaataag cactttcccg ttttagtccg agaaattcca
      481 ataccatcgg gtctttaatg agctgtttcg gatcaaccgg ctgctgctca tttctggcaa
      541 cagcaagcac actttcctta tcattactga gcaataatcg ctcgtataga ctactattaa
      601 tttgccgttc cagctggcgt gaactccaat tattcttaca actttcagcc aaataaaatt
      661 cccttttgtc agaatcggag atttgaatta gacgacgata ttgtgtccaa ttcaattgcg
      721 accgcagtgc ggtcgcaatt ggaaacgcac gataaaactg gcgataacgc tctaattgac
      781 ggctacaaaa accacttcca aattctggct caagctcttt tgcgagcatt ttaatcaggt
      841 atgttccata atcagcacgc tcttgccctt gttgctcctg ttcaaaaatc cgttttccga
      901 tgtgccaata catcagcaca cgttggaaat ctaccgcacg aaccgcattt tcacgagctt
      961 gagcaataat ttgtttaata tcttagatca tcgttgtttc tattaacata aaagcctccg
     1021 ttttggatta agtttatgct taatctatcc ggaggcaatt tctttcactc aacttttcga
     1081 gtcatatcac aattttattt tgttaaattt aattttgcac aaacgcttgc gtttgaacat
     1141 cagctttgct gatggctcga agagcgaacg tagtgagtaa atttttgctc taatttgagc
     1201 gcttgttggg caagttcttc gacttggtca tagaaatgca ccgcctgtaa acgatgcacc
     1261 aacaccggtc tttcttggat taaattctca accaaatgtt gattattttg ctcaaattga
     1321 gcttttactt tacctagcaa ttttttagca agatcggtgc tagtttgagc cactacatcg
     1381 ccaacaaaag gggaaagcag ttcggcaggg tctttttcga gctgttcaag taatgcagtg
     1441 aaatgttgca acacttgaat atcgcccgtc agcaccaacg atttattatt aatcagctcg
     1501 gttaattttt gcttatccgc tagtttcggc agggtgtcaa atgccagttg caccgtacaa
     1561 tctgcttccc cctcgtagtt gccaagccaa tcggttcggc tttcgctaaa caacacaaaa
     1621 aagtcgattt taggcgaagt tagggcgatt tttagcactt ttccggacaa tttacgcaaa
     1681 ttcggctcaa tatgaggcga gcgtttaagt aacgcattaa aagcggtttc aatcagacca
     1741 aaggtaaatt gcgggagcat taattgttgt tttacattat ttaacattcg tacctactta
     1801 ccctcttcac ttttgggaga aggatagatt gttcttcatt aagaagcaaa atcagggaga
     1861 ggggtatttg caaaatattg aataaatatg accgcttgcc ccctctctct gcttatttcg
     1921 ctaaacgctc ataagcggtc tctctcccac aaagggagag agtcatttaa gagaaaacta
     1981 aaatttataa cctctgtgta atgccacaat gccggcactt aagttgtagt aattcacgct
     2041 ttcaaagcca gtttgttcca tcattgcttt tagctcgtct tgtttcgggt gcattcggat
     2101 cgattcagct aaatagcggt aactttcggc gtcatttacc accacttcgc ccactttcgg
     2161 caaaatattg aatgagtaga aattataaag ctggctgatt gggtcgataa tcggttttga
     2221 gaattccaac acaagtaagc gaccgcccgg ttttagcacg cggaacattg aacgtagggc
     2281 tttgtctttg tcggtaacat tacgcaagcc aaaactaatc acaatacaat caaaagtgtt
     2341 atcagcaaaa ggcaagcatt cggcattcgc ttgaacgtag ctcacatttc caaccacgcc
     2401 taaattgcgt aatttttccc gcccgacttc caacattgag ctgttaatat cggctaaaat
     2461 cacctcacca ctttcaccaa caatgcgaga gaattttgcg gtaaaatcac ccgtgccgcc
     2521 ggcaagatcc agcacttttt gccctttacg cacgccactg caatcaatgg taaagcgttt
     2581 ccacactcga tgaatgccga aagaaagcaa atcgttcatt aggtcatatt tgcctgccac
     2641 cgagtgaaac acatttgcga ccagttgctg tttttcttct ttcgctacgg ttttaaaccc
     2701 aaagtgggtg gtttctgccg attgttgcac ttcttgttgc acttcatctt taggtgctaa
     2761 ttgttctaca tcgctcattt tcttgtctct agttgcaaaa tttaggcgta accatagcaa
     2821 aaaagcggtc gtttttcttt aactttttgc aaattttctt aagtaaataa ccgctttctt
     2881 gactagaaaa gccaaattct gtaacattcg cacactattt tttcttgagg attacacatt
     2941 ttggacagtc atagtcgata cttacccgtt taacctatct tgtctaacct caatgttacc
     3001 gacaaggtcg gtaatgctcg cctagtatct ttgttccttt ttgttccaag cctcctaact
     3061 atttggagct aaacatgatc cgtgcttttg gactagataa cgcatgttta atcagcgttg
     3121 atgaaactaa cccaacttta ttaaatgatg cgatttggat tgatctcatc gatccatcag
     3181 aagctgaacg aaccgtatta gaaaaccgct tagaccaaac actcgctgaa gaacgtgaat
     3241 tggaagactt agaggcgtct gcccgtttct ttgaagatga agacggtttg cacctgcact
     3301 cgttctttta ctgcttagat gatgacgatt acgccgatat tgccactgtc gccttcacta
     3361 ttcgtgacgg gcgtttgttt accttgcgtg atcgggattt acccgcattt cgcttgtatc
     3421 gaatgcgttc ccgccgtgag aaattgattg acagcaacgc ctatgaactc ttactcgatt
     3481 tatttgagac caaaatcgag cagcttgcag acgtaattga agaagtctat gctgacttag
     3541 aaaaattaag tcgggttatt ctaaacggtc agcaagaaaa tgagaatttc aatgatgccc
     3601 tttctcgctt aactgaattt gaagatgcaa gttcaaaagt gcgtttatgt ttgatggata
     3661 cgcaacgggc gttaagtttc ttactgcgta aaacccgttt gccgaataat cagctagaac
     3721 aagcccgtga cattatgcga gatattgaat ctctgcaacc gcacaatgaa tccttgttcc
     3781 aaaaagtgaa ttttttaatg caagcggcaa tgggttatat caatattgag cagaaccgaa
     3841 ttatgaaatt cttctcggtg gtgtcagtga tgttcctacc tgctacgctg gttgcttcta
     3901 cttacggtat gaactttgaa tttatgccgg aacttcattt ccaatatggc taccctatgg
     3961 cgattggttt aatgattgtt gccgcctccg ccccttatct ttattttaaa cgtaaaggtt
     4021 ggttataatt tatttttaaa gcctatagtt gaataactta ggctttctta ttttaatgaa
     4081 tatgttcacc tc
//
LOCUS       DANLZC010000175         4052 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.175, whole genome shotgun sequence.
ACCESSION   DANLZC010000175 DANLZC010000000
VERSION     DANLZC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.175"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            98..739
                     /gene="sodA"
                     /locus_tag="PXE44_002341"
     CDS             98..739
                     /gene="sodA"
                     /locus_tag="PXE44_002341"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDL5589802.1"
                     /translation="MAYTLPELGYAYDALEPHFDAKTMEIHHSKHHQAYINNANAALE
                     AHPELLEKCPGALIKDLSQVPAEKRIAVRNNVGGHVNHTLFWKGLKTGTTLQGALKEA
                     IERDFGSVEAFQSEFEKAATTRVGSGWAWLVLEEGKLAVVSTANQDNPLMGKEVAGVS
                     GYPILVLDVWEHAYYLNYQNRRPDFIKAFWNVVNWDEAARRFEEKVATCGCAK"
     gene            complement(802..1926)
                     /gene="alr"
                     /locus_tag="PXE44_002342"
     CDS             complement(802..1926)
                     /gene="alr"
                     /locus_tag="PXE44_002342"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL5589803.1"
                     /translation="MKPATATIDSQALRHNIRLIKSFAPQQKLLAMIKANAYGQGLLP
                     AAGTLADMVDGFGVARLREALEIQETGYTGKIVLVEGFFDREELLKTLSRKFDTVIHN
                     LEQLELLEQVAKEWQAEQQKGFWTRKAKIYFPINIWLKIDTGMHRLGIHPEQVDHFVE
                     RLKNCALVNSISFVSHFSRADELDCGYTEKQIAVFEQATQKYQDHDRSISASSGILYW
                     KQAHYDWVRPGIIMHGISPHYTPITDLGFKPVMTLSSSLIAVRTHKAGEPVGYGGAWV
                     SPKDTKLGVTAMGYGDGYPRNAPEGTPVLINGRIVPIVGRVSMDMLTVDLGAESQDKV
                     GDKAIFWGEQLLIEDVAKHIGVISYELITKLTPRVIFEYK"
     gene            2056..4017
                     /gene="recD"
                     /locus_tag="PXE44_002343"
     CDS             2056..4017
                     /gene="recD"
                     /locus_tag="PXE44_002343"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDL5589804.1"
                     /translation="MLNLLLELKQEKAISDLNYQFARFIDNKQKPYGYSPLEQDLAVL
                     AAALVSYQVGLGHTALRLENLSLSSLFELNSKPQTYHLLEQILQKIEQIPPLEWQSVL
                     KNHIAFSYSPETIKPMLFQNSLLYFYRYWQAEHRIAGYLQQAVDFSEKFANTELNQSI
                     LNQLFTGKAQTIDWQKIAVATALEKQFSLISGGPGTGKTTTVVKLLLGLQLKQQALQK
                     PYLQIALAAPTGKAAARMKESLESKLAEEKGLSEELKRSIPTEAVTIHRLLGARPLTE
                     KTKYHTKNPWHYDLVVLDEASMIDLSMMEKVVQALKPNSRLIMLGDKDQLASVEAGSI
                     MGELGSFIDNGYSQSHCDYLKAVTDYQIEAGNALPIGDCLSHLKHSYRFGEKAWIGEL
                     ANAVNDQKINLSWEIFDKYKNSDKLENFCYPETKETTDKSSWIEKSVQMVVDKAVELY
                     RDYLQAVQKREKNPDSVSVKAIFEKFQKVRILSALRVSELGVERLNMAIAEGLRKASL
                     VKFNHSRDRYIGKPILMTENMPINSIASGDVGIILPDENGESRVYFDSLQENREHHSL
                     PLGRVSNYEAAYIMTVHKSQGSEFEHTVLVLPHSISPVLTKELIYTAITRAKDKFTLF
                     GSEKVWKYSVGAKTERQSGLKEQIRGKFS"
BASE COUNT         1268 a          782 c          875 g         1127 t
ORIGIN      
        1 tagatcaaaa aaatgtgcat tttttgtgtg tttcttgata aaatgtactc ggagtttaac
       61 tctgattaac ctatttacta tttgagagga attaactatg gcatatacat taccagaatt
      121 aggctacgct tatgatgcgt tagagccaca tttcgatgct aaaacaatgg aaatccacca
      181 ttctaaacat caccaagctt acatcaacaa tgcaaatgca gcattagaag ctcaccctga
      241 attattagaa aaatgtccgg gtgcattaat caaagatttg agccaagtgc ctgcagaaaa
      301 acgtattgca gtgcgtaaca acgtaggtgg tcatgtaaac cacactttat tctggaaagg
      361 tttaaaaaca ggcactacct tacaaggtgc attaaaagaa gcgattgagc gtgatttcgg
      421 ttcagttgaa gctttccaat cagaatttga aaaagcagcg actactcgtg ttggttcagg
      481 ctgggcgtgg ttagtattag aagagggtaa attagccgtt gtttcaaccg ctaaccagga
      541 caacccacta atgggtaaag aagtggcagg cgtttcaggc tatccgattt tagtcttaga
      601 cgtttgggaa cacgcttact acttaaacta ccaaaaccgc cgtccggatt tcattaaagc
      661 attctggaac gttgtaaact gggacgaagc cgctcgccgt tttgaagaaa aagtagcaac
      721 ctgcggttgt gcgaaataat attttcgatc ttgaataaga tacaagcggt aaaatttgtt
      781 gaaaatttta ccgcttgttg tctatttata ttcaaaaatc acacgaggag tgagtttagt
      841 aatcagctca tagctaatca cgccaatgtg tttcgccaca tcttcaatta atagttgctc
      901 tccccaaaaa atggctttat cgcccacttt atcttggctc tctgcaccta aatcgaccgt
      961 cagcatatcc atcgaaactc gaccgacaat cggcactatt ctaccgttaa tcaacacagg
     1021 ggtaccttca ggagcattgc gagggtagcc atcgccgtag cccattgcag tcacgccgag
     1081 tttggtatct ttagggctta cccaagcacc accatagcct acaggctcgc cggctttatg
     1141 ggtacgcaca gcgatcaggg aagaggagag cgtcattacc ggcttaaaac ctaaatcggt
     1201 aatcggcgta tagtggggag aaatgccgtg cataataata cccgggcgaa cccaatcata
     1261 atgagcctgt ttccaataaa gaataccgct tgaggcggaa atactgcggt cgtgatcctg
     1321 atatttttgc gtagcttgct caaacaccgc aatttgtttt tcggtatagc cacaatcgag
     1381 ttcatcggca cggctaaaat gactcacaaa gctgattgaa ttgaccaagg cacaattttt
     1441 caaacgttca acaaagtgat cgacttgctc cgggtgaatg cctaagcggt gcattccggt
     1501 gtcgattttt agccaaatgt taatcgggaa gtagattttc gctttacgag tccaaaagcc
     1561 tttttgttgc tctgcctgcc attcttttgc tacttgctcg agtaattcca gttgttcgag
     1621 gttatgaatg acggtatcaa acttgcggga aagggttttg agtagctctt ctcggtcaaa
     1681 aaaaccttca actaacacaa ttttgcctgt gtatccggtt tcttgaattt ctaaggcttc
     1741 acgcaaacgg gcaactccaa agccatcaac catatctgct aatgtgccgg cagccggcaa
     1801 taagccttgc ccgtaagcat tggctttgat catcgccaat aatttttgct gtggagcgaa
     1861 agatttgatc aggcgaatat tatggcggag ggcttgacta tcaatggttg ctgttgctgg
     1921 tttcatactt atctacatag ttaaaatcac aagaaaggct aaattatacg cctaaaataa
     1981 aaaatccctt agaaaataat gcaaaaaaga ttagtattat cacttaatgt taggtggtcg
     2041 atttaaggtt tgattatgct gaatttatta ttggaactta aacaagaaaa ggcgatttct
     2101 gacttaaatt atcaatttgc ccgttttatt gataataagc agaaacccta tgggtattcg
     2161 cctttggagc aagatttagc tgtgttagcc gctgcgttgg tttcttacca agtgggactt
     2221 gggcatacgg cattacgttt ggaaaatctc agtctttctt ctctttttga attaaatagc
     2281 aagccacaaa cttatcattt gctcgaacaa attttgcaaa aaattgagca aattccaccg
     2341 cttgaatggc agagtgtgct gaaaaatcat atcgctttta gctattctcc cgaaacaatt
     2401 aaaccgatgt tattccaaaa cagcttgctc tatttttatc gctactggca agcggagcat
     2461 cgcattgccg gttatttaca acaagcggtt gatttttcgg aaaaatttgc aaataccgaa
     2521 ttaaaccaaa gcattctcaa ccaactattc actgggaaag ctcaaactat cgactggcaa
     2581 aagattgccg ttgcaacagc attagaaaag cagtttagct taatttcagg tgggccggga
     2641 accggaaaaa caactacggt agtaaaatta ttactcgggc tgcaattaaa acagcaagca
     2701 ctacagaaac catatttgca aatcgctctg gctgcaccaa ccggaaaagc agcagcaaga
     2761 atgaaagagt cgctggaaag caagttggcg gaagaaaagg ggttatctga ggagttaaaa
     2821 cgctctattc caactgaagc agtgactatt catcgcttgc ttggtgcgag accattaact
     2881 gagaaaacca aatatcatac caaaaatccg tggcactatg atttggtcgt attagatgag
     2941 gcttcaatga ttgatttatc aatgatggag aaagttgttc aagcgctcaa accaaactct
     3001 cgcttgataa tgctgggaga taaagatcag cttgcttctg tggaagctgg ttcaattatg
     3061 ggggaacttg gctcttttat tgataatggt tatagccaat cccattgcga ttacttaaaa
     3121 gccgttaccg actaccaaat tgaagccggt aatgcgttgc cgattggtga ttgtttatct
     3181 cacttaaaac atagctatcg atttggcgaa aaagcctgga ttggagagct tgcaaatgca
     3241 gtaaatgatc aaaaaattaa cctttcgtgg gagattttcg acaaatataa aaacagtgat
     3301 aagttagaaa atttctgtta tccggaaacg aaagagacaa cggataaatc aagctggatt
     3361 gaaaaaagtg tgcaaatggt ggtggataaa gcggttgaac tttatcgtga ttatttacaa
     3421 gcggttcaaa aacgggagaa aaatccagat tcagtgagtg tgaaagcgat ttttgagaaa
     3481 tttcaaaaag tccgaatttt atcagcctta agggtaagtg aactgggggt tgaacgtctt
     3541 aatatggcga ttgcggaagg cttgcggaaa gctagcttag tgaaatttaa tcatagccga
     3601 gaccgctata tcggaaaacc gattttaatg acagaaaata tgccgataaa tagcatagcc
     3661 agcggtgatg tcggcatcat tttgccggac gaaaatggag aaagccgagt ttattttgat
     3721 tccttacagg aaaatagaga acaccatagt ttgcctctag gtagggtttc aaattatgaa
     3781 gcagcttata ttatgactgt gcataaatcg caaggttctg aatttgaaca tacggtattg
     3841 gtgttgcccc attcgatttc cccggtgctg acgaaagagt tgatttacac cgcaatcaca
     3901 agggcaaaag ataaatttac cttatttggt agcgaaaaag tttggaaata cagtgttggt
     3961 gccaaaaccg aaagacaaag tgggctaaaa gaacaaataa gaggcaagtt tagctaaatt
     4021 aatactcgtt ttgccgtaaa aatacgcaag cg
//
LOCUS       DANLZC010000176         4034 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.176, whole genome shotgun sequence.
ACCESSION   DANLZC010000176 DANLZC010000000
VERSION     DANLZC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.176"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(57..689)
                     /locus_tag="PXE44_002344"
     CDS             complement(57..689)
                     /locus_tag="PXE44_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 family protein"
                     /protein_id="HDL5589805.1"
                     /translation="MKNLEQLLDKQSAWLILRDWFTQAKNHYEILASYPEDAGSALVG
                     MQLSTRSPLGAMVYETGGVFIDHGWLRILGSGNEKLPRGLFDWNFGKTFKQSGEQPSH
                     LLVADDAIGGYFAINAGGIGSDLGKVYYYHPKAKKWENTQLGYSEFLGWALAGDLNHF
                     YQDLRWENWQTDVEKLQGHQVLDLTSKIAVPIERHYQGEFAPENMGYSVA"
     gene            complement(743..1264)
                     /gene="hscB"
                     /locus_tag="PXE44_002345"
     CDS             complement(743..1264)
                     /gene="hscB"
                     /locus_tag="PXE44_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HDL5589806.1"
                     /translation="MNNPFTLFDLPVQFQLDNAQLSERYLALQKQLHPDNFAHKSSAE
                     QLEAVQKSADVNEALNILKDPILRAEAMIEIHTGEAKNLEEKSMRDVDFLMQQLEWHE
                     KLETIEHRKDETELTVFLKQIKAEQKAVLEQLEQTLNTADWQKANALTDKLRYFKKLI
                     IQIEKVEEKFFDY"
     gene            complement(1274..1597)
                     /gene="iscA"
                     /locus_tag="PXE44_002346"
     CDS             complement(1274..1597)
                     /gene="iscA"
                     /locus_tag="PXE44_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDL5589807.1"
                     /translation="MSITLTEAAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEF
                     VDVLNEDDQVFEQHGVKVIVDEKSLTYLNGTQLDFVKEGLNEGFKYTNPNVKNECGCG
                     ESFSV"
     gene            complement(1706..2089)
                     /gene="iscU"
                     /locus_tag="PXE44_002347"
     CDS             complement(1706..2089)
                     /gene="iscU"
                     /locus_tag="PXE44_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDL5589808.1"
                     /translation="MAYSEKVIDHYENPRNVGTFDKEASDTGTGMVGAPACGDILRLQ
                     IKVNDQGIIEDARFKAYGCGSAIASSSLITEWVKGKSLEEAGAIKNSDIAEELELPPV
                     KVHCSILAEDAIKAAIADYKEKQAK"
     gene            complement(2216..3436)
                     /locus_tag="PXE44_002348"
     CDS             complement(2216..3436)
                     /locus_tag="PXE44_002348"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HDL5589809.1"
                     /translation="MKLPIYLDYAATTPMDERVAKKMMEYMTKDGIFGNPASRSHKFG
                     WEAEEAVDVARNQIADLIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITVKT
                     EHKAVLDTCRQLEREGFEVTYLDPEEDGILDLAKLKAAIRPDTILVSVMHVNNEMGVI
                     QPIKEIGAICRAKKIIFHVDATQSVGKIEVNVQDLNVDLMSFSSHKLYGPKGIGGLYV
                     CRKPRVRLEAIIHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMARLTVLRDR
                     LYNGFKDIEEVYVNGSMEEGKRIGSNLNISFNFVEGESLMMSLRDIAVSSGSACTSAS
                     LEPSYVLRAIGRDDELAHSSIRFTLGRWTTEEEIDHTIEIVKKAIVKLRELSPLWEMF
                     KEGVDLSKIEWNHH"
     gene            complement(3491..3943)
                     /gene="iscR"
                     /locus_tag="PXE44_002349"
     CDS             complement(3491..3943)
                     /gene="iscR"
                     /locus_tag="PXE44_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDL5589810.1"
                     /translation="MKLTSKGRYAVTAILDIALHGKLEPVSLADISDRQAISLSYLEQ
                     LFAQLRRDGIVQSVRGPGGGYQLGKKPEEISVGMIISAVNENIDVTKCKGSGNCRKDS
                     QCLTHSLWKRLEDQIEQFLGTISVADLAAEHADHDCEKEHCHDHPHHH"
BASE COUNT         1122 a          891 c          714 g         1307 t
ORIGIN      
        1 tttcgttcta aaaaaataag ctaaacagcc tatatttcag cctctcttac aaacacttaa
       61 gccaccgaat atcccatatt ttcaggggca aattcgcctt ggtagtggcg ttcaatcgga
      121 acggcgattt tacttgtaag atccagcact tgatgccctt gtaatttctc tacatcggtt
      181 tgccagtttt cccaacgcaa atcctgataa aaatggttta aatctccggc taatgcccag
      241 cctagaaatt ctgaataacc taattgggtg ttttcccatt ttttagcttt tggatgataa
      301 taataaactt tgccaagatc tgagccgatt ccgccggcat taatggcaaa atagccaccg
      361 attgcatcat ccgctaccag caagtggctt ggttgttcgc cagattgttt aaaagttttg
      421 ccaaagttcc aatcaaataa gcctcgaggc aatttttcgt tgcctgagcc taaaatgcgt
      481 aaccaaccgt ggtcaataaa tacgccaccg gtttcataca ccattgcccc tagcggtgag
      541 cgagtagaaa gttgcatacc aaccaatgca ctgccggcat cttcagggta actcgctaaa
      601 atttcgtaat gatttttggc ttgggtaaac cagtctctta aaattaacca agccgattgt
      661 ttatctaata attgttctaa atttttcatt tgcaaaattt ccttagaatt tgaccgcttg
      721 cgaaaaccat acaaatgtag gattaatagt caaagaattt ttcttctact ttctcaatct
      781 gaataattaa ttttttaaag taacggagct tgtcggttaa cgcattggct ttttgccagt
      841 cggctgtatt taacgtttgt tcaagctgtt ctaatactgc tttttgttcc gctttaatct
      901 gttttaaaaa gacggtcagt tcggtttcgt ctttacgatg ctcaatggtt tccagttttt
      961 cgtgccattc gagttgctgc attaagaaat ccacatctcg catacttttt tcttcgagat
     1021 ttttggcttc acctgtatgg atttcaatca tcgcttctgc ccgtaaaatc ggatctttaa
     1081 gaatattgag tgcctcattg acatcagcag atttttgcac cgcttctaac tgttcagcac
     1141 ttgatttgtg ggcaaagtta tctgggtgaa gctgtttctg taaagcaaga taacgctcag
     1201 aaagctgggc gttatcaagt tgaaattgaa cgggtaaatc aaataaagta aaagggttat
     1261 tcattacagt tccttacaca ctaaaacttt cgccacagcc gcattcgttt ttaacattcg
     1321 ggttggtgta tttaaagcct tcgtttaagc cttctttcac aaaatccagc tgcgtaccgt
     1381 ttaagtaagt caggcttttt tcatcgacaa taacttttac gccgtgttgt tcgaaaacct
     1441 gatcgtcttc atttaataca tcaacaaatt caagcacata ggcaagcccc gaacaaccgg
     1501 aagttttcac accaagcctt agcccgatac ctttacctct gttttctaag aatgtacgaa
     1561 ctcggtttgc ggcagcttct gttaaggtaa tgctcatagt tttccttatg tgaatggatt
     1621 aaatcaagat agaaatagca aatgcaagcg gttaaatttt gcaaattttt tgcaaaaatc
     1681 gaccgcttgc attgattgta tcatattact tagcttgttt ctctttgtag tctgcaattg
     1741 cagctttgat tgcatcttct gctaagattg aacagtgaac ttttaccggt ggtaattcta
     1801 actcttcagc gatatcactg tttttaatcg cacctgcttc ctctaatgat ttacctttta
     1861 cccattcggt aatgagagaa cttgatgcga ttgcagaacc acaaccgtat gctttaaagc
     1921 gtgcatcttc aatgatacct tgatcgttca ctttaatttg taaacgtaaa atatcaccac
     1981 aagccggtgc tcccaccata cctgtaccgg tgtctgatgc ttctttatca aaagtcccta
     2041 cgttgcgtgg gttttcgtaa tgatcaatta ctttttcgct atatgccatt tttgtattcc
     2101 ttttatttcc tatttccggc gaaacctatt tcgcccctac attcaattta ctatttgtga
     2161 tgaaagctta ttttgtaggg gcaaatatta tttgcccttg caaaattttt ctgaattagt
     2221 ggtgattcca ttcgatttta gacaaatcta caccttcttt gaacatttcc caaagaggcg
     2281 aaagctcacg caatttcacg atagcttttt tcacgatttc aatagtatga tcgatttctt
     2341 cttcagtcgt ccaacgacct aaagtgaaac ggattgagct gtgagctaat tcatcgtcac
     2401 gaccgattgc acgtaacaca taagatggct ctaaacttgc agaagtacaa gctgaacctg
     2461 atgatactgc gatatcacgt aatgacatca ttaacgattc gccctcaaca aagttaaagc
     2521 tgatgtttag gtttgaaccg atacgtttgc cttcttccat tgaaccgttt acatatactt
     2581 cttcaatatc tttaaagccg ttatataaac gatcacgtaa cacggttaaa cgagccattt
     2641 ccgttgccat ttcttcttta gcgatacgat atgcttcacc catacctaca atttggtgaa
     2701 caggcaatgt acccgaacgc ataccacgct cgtgaccgcc accgtgaata attgcttcta
     2761 aacgcacacg tggtttacga caaacatata gaccgccgat accttttggt ccgtataatt
     2821 tgtggcttga gaaagacatt aaatcaacat ttaagtcttg tacattcact tcgattttac
     2881 caacagactg ggtcgcatca acgtggaaaa tgattttctt cgcacggcag attgcaccga
     2941 tctctttaat cggttggatt acgcccattt cgttatttac gtgcattacc gaaactaaaa
     3001 ttgtgtccgg acgaatagcc gcttttaatt tagctaaatc taaaataccg tcttcttcag
     3061 ggtctaagta agtcacttca aaaccttcac gctctaactg acggcaagta tctaatactg
     3121 ctttatgttc ggttttaaca gtgataatgt gtttaccttt ggtttgatag aaatgagccg
     3181 cacctttgat ggctaagtta tccgactctg ttgcaccaga agtgaaaacg atttcacggc
     3241 tgtctgcacc gattaaatcg gcaatttggt tacgagccac atcaaccgct tcttccgctt
     3301 cccaaccaaa tttgtgtgag cgagaagcag ggttaccaaa gataccgtct ttggtcatat
     3361 attccatcat ttttttcgca acacgttcgt ccattggtgt ggttgctgcg taatccaaat
     3421 aaattggtaa tttcattgtt actcctagtt aaaacaaccc aagcggtcat ttttgataaa
     3481 aaatttgcaa ttagtgatga tgaggatgat cgtgacaatg ctctttttca caatcgtgat
     3541 cagcgtgttc tgcggctaaa tccgcaactg aaattgtccc taaaaactgc tcaatttgat
     3601 cttctaaccg cttccacaaa gaatgcgtta aacactgaga atctttacgg caattaccac
     3661 tacctttaca tttggtgaca tcaatatttt cattcacggc ggagatgatc atacccacgc
     3721 taatctcttc cggtttttta cctaattgat acccaccacc cggtccacga acgctctgca
     3781 cgataccgtc acggcgaagt tgagcaaaca gttgctctaa ataagacagt gaaatcgctt
     3841 ggcgatctga aatatcggct aagctaaccg gctctagttt tccgtgtaaa gcaatatcta
     3901 aaatggcggt tactgcataa cgcccttttg aggtaagctt cataaaatat cctcattgat
     3961 tatccagaaa atcttctgaa acagttgcta aattttgata taccccacca atttagtcaa
     4021 tcatttagga ctaa
//
LOCUS       DANLZC010000177         4023 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.177, whole genome shotgun sequence.
ACCESSION   DANLZC010000177 DANLZC010000000
VERSION     DANLZC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.177"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(41..301)
                     /locus_tag="PXE44_002350"
     CDS             complement(41..301)
                     /locus_tag="PXE44_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589811.1"
                     /translation="MPRNELTKNARAIADLIHRKSATVTHKKLARAIGLSESQFSRTF
                     ADNVEMVAVIIDYLSIELADKDELLELKEKRYKRINLEEATT"
     gene            complement(322..528)
                     /locus_tag="PXE44_002351"
     CDS             complement(322..528)
                     /locus_tag="PXE44_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5589812.1"
                     /translation="MNKIAEIRNQIGISQAQLAVQIGWGQPRIANYETGNRTPSIYVA
                     QKIVEGLNELGANVRIDDVFPVGN"
     gene            658..1317
                     /locus_tag="PXE44_002352"
     CDS             658..1317
                     /locus_tag="PXE44_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDL5589813.1"
                     /translation="MTTLGSRIKAYREQLGISQYEVAERCTSIDNRDKNNKWGQSRIA
                     NYERNNRTPDLDDIEILSKVLGVSPETLAFDTNVQLARPTKSNSYPLISNIQAGLWTE
                     AFDFKDSEGYDYIDTEIDAGPDAFFLRISGMSMEPKFSEGDLVLIDIRKRPHPGDYVA
                     AVNGTGEATLKRYRELGEWSESGNPHFELIPLNPDFPTLSSMKQDIRIIGVAVEHRSY
                     L"
     gene            1368..1748
                     /locus_tag="PXE44_002353"
     CDS             1368..1748
                     /locus_tag="PXE44_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589814.1"
                     /translation="MNNKRIKPEQFADFITQRTGRNLTCPICNSQEHYLHDGYDTFTE
                     LCGEKIIGVPTIPYRIEPSPDEVMKFAAPEFHSLHFNEREGTKYWLDQKARTAVIVTC
                     VCCNNMLFFDRERILEWIKGSQNE"
     gene            1753..2238
                     /locus_tag="PXE44_002354"
     CDS             1753..2238
                     /locus_tag="PXE44_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589815.1"
                     /translation="MIQNDPRDAIILSLLANAVYGGHNNSQISYQETEEVNLYARLRQ
                     VEGDVREIKSNYLTKEAFYKAGGSTLIALVVTAGIALWTVYSHLDTKVEARFSKIDDK
                     FVQVETNIKNLDIRLTKVESRLDNVEQRLDNVEQRLGNMEKKIDGVDNKLDLLLQQFK
                     R"
     gene            2370..2552
                     /locus_tag="PXE44_002355"
     CDS             2370..2552
                     /locus_tag="PXE44_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5589816.1"
                     /translation="MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKE
                     IGTGLVNKIKKDLDLK"
     gene            2591..3010
                     /locus_tag="PXE44_002356"
     CDS             2591..3010
                     /locus_tag="PXE44_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5589817.1"
                     /translation="MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGL
                     ATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMKVFLLNEMIKQGVRKAEMARRLD
                     VHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH"
     gene            3346..3990
                     /locus_tag="PXE44_002357"
     CDS             3346..3990
                     /locus_tag="PXE44_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5589818.1"
                     /translation="MFGHIWQYAREWGYTTLASPNTGMKRFPIRKREIYVENNIFEMV
                     YEVADQDIKDLMDLAYLTGQRPVDLVNIQRDHIFDGYLHIVQQKTQAKLRIEIVGKLA
                     DIIQSRLEKTTRNFLFTTSKGEKMTSVKLGRRFTYIREQAAKKHPEYANEIKEFQFRD
                     LRAKSGTDKALAQGEEAARQQLGHTSVQMTKVYIRKAPIVTPIQESVSSQKHKP"
BASE COUNT         1379 a          751 c          740 g         1153 t
ORIGIN      
        1 atttgaattt ttctaaaaaa tttttcattg ttggtaactt ctatgttgtc gcttcttcga
       61 gatttattcg cttatatcgt ttctctttca gctctagtaa ctcgtcttta tccgcaagct
      121 caatacttaa gtaatcaata atcaccgcca ccatttccac attatctgca aatgtacggc
      181 taaattgtga ttcactcagt ccgattgctc ttgctagttt cttatgcgtc accgttgcgg
      241 attttcgatg aatcaaatcc gcaatcgctc ttgcattttt tgttaattca ttgcgtggca
      301 ttgcaagcct cttggggtaa attagttgcc aacggggaaa acgtcatcaa ttcgtacgtt
      361 tgcacctaat tcgttcaatc cctcaacaat tttttgagca acgtaaatag atggggtacg
      421 atttccagtt tcatagttag ctattcttgg ttgcccccaa ccaatctgaa cagctaactg
      481 agcttgactt ataccaattt ggtttcgtat ttcagctatc ttattcatac aaatttctct
      541 ttgtgattta tatcttttaa ttaaatcaca aaatgaacta attgtaaatt tcattttgtg
      601 attcacaagg atataacgga gcgtgttaaa atgaggaaaa tctaatagga gagtgctatg
      661 acaaccttag gtagtagaat taaggcttac agagaacagc taggaatcag ccaatatgaa
      721 gttgctgaaa gatgtacatc aatagataat cgagataaaa ataacaaatg ggggcaatct
      781 cgcattgcta attatgagag aaacaatcgt actcccgatc ttgatgatat tgaaatactc
      841 agcaaagtgc ttggtgtttc tccagaaaca cttgcttttg atacaaacgt tcagcttgcc
      901 agaccgacaa aatcgaactc atatccgttg atcagcaata tacaagcggg tttatggaca
      961 gaagcctttg atttcaaaga ttcggagggc tacgactata tcgatacgga aattgatgcc
     1021 ggaccggacg ctttcttttt aagaatttcc ggaatgtcga tggagcctaa atttagtgaa
     1081 ggcgatctgg tgttaatcga tattcgcaaa cgtcctcacc cgggcgatta tgtagcagcg
     1141 gttaatggca caggtgaggc tactttaaaa cgttatcggg agttaggcga atggtctgaa
     1201 tccggcaatc ctcattttga gctaataccg cttaaccccg actttccaac actcagctca
     1261 atgaaacaag atattcgtat tattggtgtc gctgttgagc atagaagcta tttgtaaagt
     1321 ccaccaaagc taacagttga attttattgg gtagaaagga agttaaaatg aataataaac
     1381 gcattaaacc tgaacagttt gcggatttta tcacgcaacg tactgggcgg aatttaactt
     1441 gccctatttg taatagccaa gaacattatt tacacgatgg ctatgacact tttacggaat
     1501 tatgcggaga gaaaattatc ggtgttccaa ccattccata ccgaatagag ccatctcctg
     1561 atgaagttat gaaatttgca gctcctgaat ttcattcttt gcatttcaat gaacgggaag
     1621 gaacaaaata ttggttagac caaaaagcga gaacagccgt tattgtcact tgtgtttgct
     1681 gtaataatat gctttttttc gacagagaga gaattttaga atggattaag ggctcacaaa
     1741 atgaatagac caatgattca aaatgatcct agagatgcaa tcatactatc cttacttgca
     1801 aatgcagtat acggcgggca taataattca caaattagtt atcaggagac ggaagaagtg
     1861 aatttatatg ctcgattaag acaagttgaa ggcgatgtaa gagaaatcaa gtcaaattat
     1921 ttgacgaaag aggcttttta taaagctggt ggctctacac tcattgcatt agtagttact
     1981 gctggaattg cattatggac agtatattca caccttgaca ccaaagttga agctcgattc
     2041 tcgaaaattg atgataagtt tgtccaagta gagaccaata tcaaaaatct tgatattcgc
     2101 ctcactaaag ttgaatctag attagataat gtcgaacaac gactagacaa tgttgagcag
     2161 cgactaggca atatggagaa gaaaatagat ggggttgata ataaactcga tctgttacta
     2221 caacaattca aaagataaaa taatattcct tgaataaacc gccctcgtgg cggttttctt
     2281 ttctgctaca aaaaaaacac aacaaaaatg ttgtaatacc aacaaaaatg ttgtattatt
     2341 tccttgctgt tttgataagg aggtatctta tgaaatacag cgagttcctg aggtacttac
     2401 ttgctcaagg ttgcgaaatt gaaaaccata ggcgaggcag tcatcgaaag gtaacactaa
     2461 acggaaaaca atcggttttt ccttatcacg ggagcaaaga gattggtaca ggtttagtaa
     2521 ataagattaa gaaagactta gatcttaaat aacaaagtcc ctcgaaagag gggcttttct
     2581 aggagtttat atgttacgct acccagttga aattacccct gatgataacg gcacatatct
     2641 tgtgacttgc cctgatattc ctgaaatggc aagcgtaggt gaggacttag aagaagccct
     2701 attagaagca caagatggct tagccacagc ccttgaattc tattttgatg atagacgaga
     2761 aataccaatg ccaagcccaa ttaaagaggg acaacatacg gttaatctta ccgtgctgca
     2821 atcaatgaaa gtatttctct taaatgaaat gattaaacaa ggtgtacgca aagcagaaat
     2881 ggcaagacga ttagatgtac acttaccaca gatagatcgc ctattagatt ttaatcattc
     2941 cacaaaagta gaatttgttg agaaagccta tggtaagcta aatcaacact ttactattct
     3001 ccctcactaa taaaataacc gcctacgggc ggttttcttt ttcctaaaat ctgcaaaatt
     3061 ctcttcaaaa acgaccgctt gcaactcaat ctgtttattt attaagcaat caaacaacct
     3121 ttctaaaaat aaattctctt taaaatcaat aaattactaa acagaactaa actttattta
     3181 ttttccacta aataaacact ttactaaatg tttaatattt actaaactac acccatcaaa
     3241 acaaacaacg ccacagacaa aggggaaacc aaaatgaacg cacaaacaac gctaaacaac
     3301 cacaaagact acattcacta agaattttta tcaaagccct tttcaatgtt cggtcatatt
     3361 tggcaatacg caagagagtg gggttacaca acgttagcta gtcctaatac aggaatgaaa
     3421 cgctttccaa ttagaaaacg tgaaatttac gttgagaaca atatttttga aatggtgtat
     3481 gaagtagcag atcaggatat taaagattta atggatctcg cttaccttac aggacaaaga
     3541 cctgtagatt tagttaatat ccaacgcgat catatatttg atggatatct ccatattgtg
     3601 caacaaaaaa cacaagccaa actgcggatt gagatagttg gcaaacttgc tgatataatt
     3661 caaagtaggt tagaaaaaac cacaaggaat ttcttattta caacgtcaaa aggagaaaaa
     3721 atgacctctg taaaattagg aagacgcttt acttatatcc gagaacaagc agcaaaaaaa
     3781 catcccgaat acgcaaatga aataaaggaa ttccaattta gagatctaag ggctaagtca
     3841 gggacagaca aagcccttgc acaaggagaa gaagctgcac gccaacaatt aggtcatact
     3901 agcgttcaaa tgacaaaagt ctatatccga aaagccccga tagtaacccc aattcaagaa
     3961 agcgtttcct cccaaaaaca caaaccttaa aacctagttt cggaacatct gagaattttt
     4021 cgg
//
LOCUS       DANLZC010000178         3963 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.178, whole genome shotgun sequence.
ACCESSION   DANLZC010000178 DANLZC010000000
VERSION     DANLZC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.178"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            158..1360
                     /gene="yedE"
                     /locus_tag="PXE44_002358"
                     /pseudo
     CDS             158..1360
                     /gene="yedE"
                     /locus_tag="PXE44_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
     gene            1396..1665
                     /gene="yedF"
                     /locus_tag="PXE44_002359"
     CDS             1396..1665
                     /gene="yedF"
                     /locus_tag="PXE44_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDL5589819.1"
                     /translation="MAFTVVQKLDKDPKDITPDYTLDTLGEPCPYPAITMLETMPQLQ
                     KGEILELLSDCSQSINNIPIDTKNHGYTLLSVEQNGTKLRYLIQR"
     gene            1765..2109
                     /locus_tag="PXE44_002360"
     CDS             1765..2109
                     /locus_tag="PXE44_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL5589820.1"
                     /translation="MNKYALLAIFWFIIGIYALLFRESTNDIPPFPHFDKVSHFALFF
                     IQIWLSANAFIRSKKGIPYFGLIIFALLYAFTSEWAQATFTSTREGSWLDGLADMLGA
                     SLSLLLVKYCKK"
     gene            complement(2172..3119)
                     /locus_tag="PXE44_002361"
     CDS             complement(2172..3119)
                     /locus_tag="PXE44_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDL5589821.1"
                     /translation="MNRITQILNIKHPIVQAPMSWLTDAHLVASVAEAGGLGFLAPHA
                     GQTTNPTSNEEVLDRMRNEIRKVKALTDKPFGVPFILSYDFSLIPLMVDLFIEERVPV
                     VLDNGWLDPEIYAKFKAAGIKIICRLPNPNLADALKAQALGADILVLTGFDEGGTLPM
                     KEVGSFNVLAEFVGKVDLPIMLAGGIADKKAVQAALTLGAEGVWIGTAFIATEECRAS
                     EKVKNWIVEANANDLLLFRTEPHYYRSLPTGLAKKCFEMSENGATRSEIAKVMNAGTG
                     MRLGMLEGDFDNGYVSVGNGISQINKIKSVKELIDELMA"
     gene            3302..>3963
                     /locus_tag="PXE44_002362"
     CDS             3302..>3963
                     /locus_tag="PXE44_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589822.1"
                     /translation="MSIRWENIKPLQGSQNSAFEELVCQLARQEFKSQGKFTRISAPD
                     GGIEAMCELPDGSVCGWQAKYFLSSFSSSQWQQIEDSFKESLKNYPKLTKYYVCVATD
                     RANANVSGKKSFLTKWEEYTQRWKDFAQSQGREIEFEFWGSFELSDLLSKPENAGKKF
                     FWFNESELSDKWFEQYNQQAINNLGVRYTPEINVDLPIAMQLESLARTQIFKKHLSDE
                     FH"
BASE COUNT         1154 a          829 c          781 g         1199 t
ORIGIN      
        1 aaaataccta ctataaagac gcatttgtaa aatgtgcatt gatatatcgc aagttttgtg
       61 tataggaaat gaactaatta ctgtgtcaat cagtcccttt cttcatcgtt ataccctctt
      121 gcaaatccga ttaatctatc cttagttaag gtgttttatg ttagcgattt ggcaaagttt
      181 taaacaaaat tatctaatca aatattggaa tcctgttgca gcagtgatcg cagctggtat
      241 tatttcagct tattatttcg gcattactgg cacttattgg gcggttacag gggaatttac
      301 ccgttggggc ggtcatctgt taaatgcttt gggtgttgat gtttcgaact ggagctattt
      361 tcaactcatc ggcttagacg gcaccatctt tacccgcatt gacggtgtga tgattctcgg
      421 tatgtttgcc ggctgtcttt ctgccgcatt atgggcgaac aacgtcaaat ggcataatca
      481 gccacataaa aaacggatta tccaagcctt gattggcggt gcgttagccg gtttcggagc
      541 ccgtttagca atgggctgta acctcgcctc tctttttacc ggtattccgc aattctccgt
      601 ccacgcttgg tttttcacct ttgcgaccgc catcggcact tactttgggg taaaagtgac
      661 tttattaccg atgtttagag tgaaattaga gctgaaaaaa ggggcagcaa aaatcaaaga
      721 aaacgatccg aaacaagcta atcgccgttt ttgggttggt atgctcattt ttatcactta
      781 tctttctgct tccctttatg taatgcaatc ctcaatcaaa cttggttttg caatgctttg
      841 cggcttagcc tttggcttat tgattgaacg agcccaaatt tgctttacct ccgctttccg
      901 tgacttatgg attaccggac gggcttatat ggcaaaagca attattttcg gcattattgc
      961 cggcacaatc ggcgtgttct cttatattca attaggtgtt tcacctaaaa ttttgtgggc
     1021 tggcccaaat gccattatcg gcggattatt attcggattt ggcattgtgc ttgccggcgg
     1081 ctgtgaaacc ggctggatgt accgttcaat ggaaggacag gttcatttta tgtgggtcgg
     1141 cgtaggcaat gtagtcggtt caaccctgct ggcttactac tgaggcgact tagctccggt
     1201 gcttaccctt gattatgaaa aaatcaacct actcaaagaa tttgggccgg ttggcggatt
     1261 attcatcaat tatggcttat taattctttg cttaatcgct gtagtttggt gggaaaaacg
     1321 cttccttgcc aaagcgaaag caaaattagc aactgcgtaa tctttttaac ttcaaccaac
     1381 aagaggaaaa tgactatggc attcaccgtt gtacaaaaac tagataaaga tccaaaagac
     1441 atcacgcctg attacacctt agatacctta ggcgaacctt gcccttaccc tgcgattacg
     1501 atgttagaaa caatgccaca gctgcaaaaa ggagagatat tggaactgtt aagcgattgt
     1561 tcccaatcga ttaataatat tccgattgat actaaaaatc acggctatac cctattaagc
     1621 gtggaacaaa acggcactaa attacgctat ttaattcagc gttaattgca aaaaattcgc
     1681 acaaaacgac cgcttgtcgt aaaatagcaa ctatttaaga gccttgtaac gcaaggctct
     1741 tttattctaa gaggtaaaag aaaaatgaat aaatacgctt tacttgcaat attttggttt
     1801 attattggca tttatgcttt actttttcgt gaaagcacta atgatatacc gccttttcca
     1861 cattttgata aagtaagcca ttttgcctta ttctttatcc agatctggtt atctgcaaac
     1921 gcctttatcc gttcaaagaa agggatacct tattttgggc taataatttt tgccttactc
     1981 tatgctttca ccagcgaatg ggctcaggca accttcacct caacccgaga aggttcttgg
     2041 cttgatgggc tagcagatat gctaggagcc tcactttcat tattgctggt aaaatattgc
     2101 aaaaaataaa gcataaaaga ccgcttgtta tatggtttag ccatactaca agcggtcatt
     2161 ttttaccaaa attatgccat caactcatca atcaattctt tcacactttt gattttgttg
     2221 atttgtgaaa tgccattgcc caccgacaca tagccattgt caaaatcccc ttctaacata
     2281 cccaaacgca ttcctgtacc agcgttcatt accttagcaa tctcactacg agttgcccca
     2341 ttttcgctca tttcaaagca ttttttcgct aatccagtcg gcagtgaacg gtagtaatgt
     2401 ggttcggtac ggaataacag cagatcattg gcatttgcct ccacaatcca gtttttcact
     2461 ttttcgcttg cacggcattc ttcggtggcg ataaatgccg tgccaatcca cacgccttca
     2521 gccccaagcg ttaaggcagc ctgcaccgct tttttatcgg caataccacc agccagcatt
     2581 atcggcaaat ccaccttgcc cacaaattcc gccagcacat taaaactgcc tacctctttc
     2641 atcggcaaag taccgccctc atcaaaaccc gtcagcacca aaatatctgc tcccaatgct
     2701 tgggctttta aagcgtccgc caaattggga ttaggcaaac ggcagatgat tttaatgcca
     2761 gcagctttga atttggcgta aatctctggg tcaagccagc cgttatccag caccacaggc
     2821 acacgctctt caatgaataa atccaccatt aacggaatca gcgaaaaatc ataagacaaa
     2881 ataaatggta cgccaaaagg cttatcggtc aatgccttaa ccttacgaat ctcgttacgc
     2941 atacggtcaa gtacttcttc gttggaagtt ggattggtgg tttgccctgc gtgtggggct
     3001 aaaaaaccaa gcccaccagc ctcggcaacg cttgctacca gatgagcgtc cgtcagccaa
     3061 gacattggcg cttggacgat agggtgcttg atatttaaga tttgggtaat gcggttcatt
     3121 ggattttcct gtttgaataa aaagaattgg acgtagttta atggttttgg atttgagagg
     3181 gaatgcgtga aaatgggaag gtggttttcg gatttggaaa attatcaatg ctaaaatact
     3241 tgaaatacac acgccaatcc tcatatcaac aaaattgaat atttaattat ggtaaaagaa
     3301 aatgtctatt agatgggaaa acatcaagcc cttacaaggt tctcaaaata gtgcttttga
     3361 agagctagta tgccaattgg cacgccaaga atttaaatct caaggaaaat ttactcgaat
     3421 ttctgcacct gatggtggga ttgaagcaat gtgtgaattg ccagatggtt ccgtatgtgg
     3481 ttggcaagcc aaatattttt taagctcatt ttcttcatca caatggcaac aaattgaaga
     3541 ttcctttaaa gaatctttaa aaaactatcc taaactaacc aaatactatg tttgtgtggc
     3601 aaccgataga gcaaatgcca atgtatctgg aaagaaatca tttcttacca aatgggagga
     3661 gtatacccaa agatggaaag actttgctca atcacaaggt cgtgaaatag aatttgaatt
     3721 ttggggaagt tttgaattat ctgacttatt atcaaaacct gaaaatgcag gcaaaaaatt
     3781 cttttggttt aacgaaagcg aattatctga caaatggttt gaacaataca atcaacaagc
     3841 cattaataat cttggtgtgc gttatacacc tgaaattaat gttgatttac ctattgcaat
     3901 gcaattagaa agcttggcaa gaacccaaat tttcaaaaaa catctgagtg atgaatttca
     3961 taa
//
LOCUS       DANLZC010000179         3917 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.179, whole genome shotgun sequence.
ACCESSION   DANLZC010000179 DANLZC010000000
VERSION     DANLZC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.179"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(302..1396)
                     /locus_tag="PXE44_002363"
     CDS             complement(302..1396)
                     /locus_tag="PXE44_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL5589823.1"
                     /translation="MKKTTLAILASALIANSALAITIYDHKETGTQIEFIGSARIKWE
                     STSDKTSPVNGKATRNHVNHAVDNNGSRFGFRLTQQVGHGFYALGRVEWRMRGDSSSQ
                     HNFDHIYDHQLYAGIGHKQYGELTYGNMTNIADEVKQTDLPNTLSLSDGLLTGSSRRT
                     LQYVYKGIEGLKLGAFYGGNSQRGNNGLDLVNKRKDIWGAAAIYNYKIDDNQSLKLAT
                     GATRERSEQSIGNYERIALAFGTAYTYGKTTLGLDLERRETEDQNAIGHKRIEKEVRT
                     VLLHRLTDQWNAYTMYAYKENKLNAVGLANDSKVKRNQFMLGTEYYIVPKHLKGFVEW
                     QATRAKHYTNDVKTAKSRNYTTVVGLRAYW"
     gene            1530..2708
                     /gene="lpxB"
                     /locus_tag="PXE44_002364"
     CDS             1530..2708
                     /gene="lpxB"
                     /locus_tag="PXE44_002364"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDL5589824.1"
                     /translation="MKQQFPVIGLVAGEVSGDILGAGLIKALKVHYPNARFIGVAGSR
                     MIEAGCQSLFDMEELSVMGLAEVVKHLPRLLKRRKQVIDEMLKLKPDVFIGIDAPDFN
                     LTVEEKLKASGIKTIHYVSPSVWAWRQKRVFKIARAANLVLAFLPFEKAFYDKFNVPC
                     RFIGHTMADAIPLEPNRTEACLALNIDESKRYMAILVGSRGSEIQFLAEPFLKAAQLL
                     KARFPDLQFLVPMVSQKRMEQFNAIKQQVAPELELNIIQGKARQAMIASECTLLASGT
                     AALEAMLCKSPMVVGYRMKPLTYWLAKKLVKTDYISLPNLLAQAPLVPELIQEACNPE
                     NLAQELSLFLADDEKNQQHKALLKQQFMQLHQSIQCNADEQAAQAVIDLLNGSTSGQI
                     "
     gene            2735..3787
                     /gene="sohB"
                     /locus_tag="PXE44_002365"
     CDS             2735..3787
                     /gene="sohB"
                     /locus_tag="PXE44_002365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDL5589825.1"
                     /translation="MWKEVLLNYGIFLLELLTVFGIIAVVVMLILESKKQNENGSISI
                     TNLSEKYKEQQKSLSGFFLSEEELKHQEKQEKKVAKEKAKAEKKRLKAGEECVESPSR
                     LFVLNFNGDMMAHEVNSLRREIDAVISLANPEKDEVLLKLESPGGVVHGYGLAASQLQ
                     RLRDRNIYLTAAVDKVAASGGYMMACVANKIVSAPFAVIGSVGVVAQVPNIHRLLKKH
                     DIDVDVMTAGEYKRTVTLVGENTEKGKQKFQQELEETHSLFKQFVARHRPQLDIEQVA
                     TGEHWFGQQAIELNLVDEIATSDDLLLNAIKEKEVIELKYKEKKKLAQKLGSQFEQSA
                     ENLLAKILHKSRSTLM"
BASE COUNT         1165 a          676 c          850 g         1226 t
ORIGIN      
        1 aataacccct catcactttt tgcagtttaa cctcttttgc aacagataat ttaaatttag
       61 gcgttttggc tgctcagtta ttgccattat gattatatta gttcacacta aaattttagt
      121 gttgtttctt aaagctgaag atgctaatag gtgagtaagc ggtttttacc actttgaaga
      181 taaatgcaat agcttcttgt ttgaaggatt agttgtattt aatcataaaa aatctgcacc
      241 ttaatcagtt ggttagccca acttttaggt gcagatcatt ctagtacgct tttcagtttt
      301 attaccaata tgctcgtaag cctacaacag tcgtataatt gcgagatttt gcggttttta
      361 catcattagt atagtgttta gcacgagttg cttgccattc aacaaagcct tttaagtgtt
      421 ttggaacaat gtaatattcc gttcccaaca taaattggtt tcttttaact ttgctatcat
      481 ttgccaagcc tacagcatta agtttgtttt ctttataagc atacattgtg taagcattcc
      541 attgatcggt taaacgatgg agtaacacag tgcgaacttc tttttcgata cgtttgtgac
      601 cgatagcatt ttgatcttcc gtttcacggc gttctaaatc taagccaagg gtagtttttc
      661 cgtaggtata agctgtaccg aaggcaagag caatgcgttc ataattgccg atagattgct
      721 cagaacgctc acgggttgca cctgttgcta attttaagct ttggttatca tcaattttat
      781 agttataaat tgctgctgca ccccaaatat ctttacgttt attgactaaa tctaagccat
      841 tgtttccacg ttggctattt ccgccataga atgctccgag ttttaatcct tcgatgcctt
      901 tgtaaacata ttgaagcgta cgtcgtgatg aaccggtaag taagccatca cttaagctga
      961 gtgtatttgg taagtcagtt tgttttactt catcggcaat attagtcata ttgccgtatg
     1021 ttaattcacc gtattgttta tgcccgattc ctgcatagag ttgatgatca taaatatgat
     1081 cgaagttatg ttgtgagctt gaatcgccac gcatacgcca ttccacacga cctaaagcat
     1141 agaatccgtg acccacttgc tgggttaaac ggaaaccaaa acgagagccg ttgttatcca
     1201 ctgcgtgatt aacgtggttg cgggtagctt taccatttac cggtgaggtt ttatctgagg
     1261 tgctttccca cttaatacga gcagaaccaa taaattcaat ttgtgtaccg gtttctttat
     1321 gatcgtaaat cgtaatagct agagctgagt ttgcgattaa ggctgatgct aaaatcgcta
     1381 atgttgtttt tttcataaaa atcactccat atgaatagat aaagggaggt agtgtatgtg
     1441 aatattcatt gctagacaat aatgttttgt tagtttatat gatgattggt tataatagag
     1501 tcacttacag atcttcaata atatttaata tgaaacaaca attccccgtt attggcttag
     1561 ttgctggtga ggtctctggc gatattcttg gtgccgggtt aattaaggcg ttaaaagtac
     1621 attacccgaa tgctcgattt atcggtgttg caggctctcg aatgattgag gctggctgcc
     1681 agtctttgtt tgatatggaa gagctttctg taatggggct agcagaggtg gttaaacatt
     1741 tacctcgttt acttaagcgt cgtaaacagg ttattgatga gatgcttaaa ttgaaaccag
     1801 atgtatttat cggtattgat gcaccggatt ttaatttaac cgttgaagaa aaactcaaag
     1861 cgagtgggat taaaacaatc cattatgtta gcccttcggt gtgggcttgg cgacaaaagc
     1921 gggtgtttaa aattgccaga gcagctaatt tagtgttggc atttctgcca tttgagaaag
     1981 cgttctatga taaattcaat gtgccttgtc gttttatcgg gcatacgatg gcagatgcga
     2041 tcccactcga accaaatcgt acagaggcat gtttagcgtt aaatattgat gaatccaagc
     2101 gatatatggc aattttggtg ggaagccgtg gcagtgaaat tcagtttttg gctgagcctt
     2161 ttttgaaggc tgctcagttg ttaaaagccc gttttccgga tttacagttt ttagtgccaa
     2221 tggtcagcca aaagcgaatg gagcaattta atgcaattaa acagcaagta gctcccgagt
     2281 tggaactgaa tattattcaa ggcaaggctc gtcaggctat gattgcgtca gaatgtactc
     2341 ttttggcatc gggaacagca gcactagagg caatgttgtg caaatctccg atggtagttg
     2401 gttatagaat gaaaccgctg acttattggt tagcaaaaaa attagtgaaa accgattata
     2461 tctccttacc gaatttattg gctcaagccc cattggtacc agaattgatc caagaagcat
     2521 gcaacccgga aaatttagct caagagttat ctctattttt agctgatgat gagaaaaacc
     2581 aacaacataa agcattgcta aaacagcagt ttatgcagct acatcaatca attcaatgta
     2641 atgctgatga gcaagctgcc caagcggtta tcgatctcct aaacggctcg acaagcggtc
     2701 aaatttagtt agttttttgt aaaaaggaaa atttatgtgg aaagaagtat tacttaatta
     2761 tggtattttt ttattagagt tattaactgt atttggcatt attgcagtgg tggtgatgct
     2821 gattttggaa tcgaaaaagc agaatgaaaa cggctcaatt tcaattacca atttatctga
     2881 gaaatataaa gagcagcaaa aatctctttc cggctttttt ctaagtgagg aagaattaaa
     2941 acatcaggaa aagcaggaga aaaaagtagc aaaagagaaa gctaaagcag aaaaaaaacg
     3001 cttaaaagca ggcgaagaat gtgtagaaag cccttcccgt ttatttgtat tgaattttaa
     3061 tggcgatatg atggctcacg aagtaaattc cctacgccgt gaaattgatg cggtaattag
     3121 cctcgcgaat ccggaaaaag atgaagtttt acttaaactt gaaagtccgg gcggagtggt
     3181 gcacggttat ggattggctg cttcgcagtt acaacgtttg cgtgatcgca atatttattt
     3241 aaccgcagcg gtagataaag tagctgccag cggtggttat atgatggcgt gtgtggcgaa
     3301 taaaattgtc tctgccccat ttgcggtgat tggctcagtg ggggtggtgg ctcaggtgcc
     3361 gaatatccac cgcttactga aaaaacacga tattgatgtg gatgtgatga ctgcaggtga
     3421 atataaacgc acggtgacgc tggtgggaga aaataccgaa aaagggaaac agaaattcca
     3481 gcaagaactg gaagaaacgc actctctgtt taagcaattt gtggctcgac accgcccaca
     3541 actggatatt gagcaggttg caacaggcga gcattggttt ggtcagcaag cgattgagct
     3601 aaatttggtt gatgagattg caaccagtga tgatctgctc ctcaatgcaa ttaaggaaaa
     3661 agaagtgatc gagctaaaat ataaagagaa gaaaaaactt gctcagaaat tgggttctca
     3721 attcgagcaa tcagcagaga acctgctcgc taagatttta cataaaagtc gttcaacgct
     3781 gatgtagcgt atatattatt aaagggctat cgtttatata gccccttatt ttatcagtta
     3841 gtaatagaat gaagtatttg ttcactattt aaatgacctg gaatactttt ctttaaattg
     3901 agtgaagaat ctaacaa
//
LOCUS       DANLZC010000180         3843 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.180, whole genome shotgun sequence.
ACCESSION   DANLZC010000180 DANLZC010000000
VERSION     DANLZC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.180"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            254..586
                     /locus_tag="PXE44_002366"
     CDS             254..586
                     /locus_tag="PXE44_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF413 domain-containing protein"
                     /protein_id="HDL5589826.1"
                     /translation="MADSFSVTRRFFDDKNYPRGFSRHGDYTIRESQTLEQFGQACLA
                     LESGERKPATQEEKRFVAVMKGEEVAETDIEKAWLKYRTLTSKTKRIYTLSGNAGSDA
                     GDDFSAAE"
     gene            651..1742
                     /gene="trmA"
                     /locus_tag="PXE44_002367"
     CDS             651..1742
                     /gene="trmA"
                     /locus_tag="PXE44_002367"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDL5589827.1"
                     /translation="MTLPIEQYSDLLAKKTENLTALLKPFNPPAIEVFASKPSHFRMR
                     AEFRVWHEEGELYHIMFNPETKARYRVDSFPIASELINHMMTALLAEIKGNELLTRKL
                     FQIDYLSTLSGEIAVSMLYHKSLNEEWVEQANALKSRLEQQGFKVQIIGRANKQKIAL
                     DCDYVEEILPVHGKNYVYRQVENSFTQPNAEMNIKMLEWARNCTKNSEGDLLELYCGN
                     GNFSIALAENFRKVLATEISKSSVQSAQYNIEKNGIVNLQIIRMSAEEFTQAMNGVRA
                     FYRLKGIDLNAYECNTIFVDPPRAGLDQATLNMVQAYERILYISCNPETLAENLQQLS
                     QTHRIEKAALFDQFPYTHHIESGVWLIRK"
     gene            1834..1923
                     /locus_tag="PXE44_002368"
     tRNA            1834..1923
                     /locus_tag="PXE44_002368"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1868..1870,aa:Ser,seq:tga)
     gene            2079..2450
                     /locus_tag="PXE44_002369"
     CDS             2079..2450
                     /locus_tag="PXE44_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDL5589828.1"
                     /translation="MKYLYIICGFICIGLGIIGAVLPGLPTTPFLLLALFCFTKGSER
                     IRQWFIQTKLYKKYLQEYDEKRAMTMKQKITILAISFPFTLFAFFALPHIGGKIALVV
                     LVIIQYWYFFCKMKTLEPENA"
     gene            complement(2447..3358)
                     /gene="xerC"
                     /locus_tag="PXE44_002370"
     CDS             complement(2447..3358)
                     /gene="xerC"
                     /locus_tag="PXE44_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL5589829.1"
                     /translation="MTTSNPLFQQTRPYWDFLRIEKQVSPHTLSNYQRQLMAVSELFS
                     QAGIENWEEVESSSVRWMLSQSHKQGLSAKSIGLRLVALRQWFVYLIKIGRMSANPTL
                     GVKSPKVGKHLPKNIDAEQVGQLLAIESDEPSDIRDIAMMELMYSSGLRLSELQGLDL
                     GDMDLSAREVRVQGKGSKERIVPIGSQALKALNRWLEMRLQFKPQDNAVFLNKRGGRL
                     SHRSIQLAMKKWGERQGLESHLHPHKLRHSFATHMLEASGDLRAVQELLGHSSLSTTQ
                     IYTHLDFQHLAKIYDSAHPRARRKQED"
     gene            complement(3425..3715)
                     /locus_tag="PXE44_002371"
     CDS             complement(3425..3715)
                     /locus_tag="PXE44_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5377 domain-containing protein"
                     /protein_id="HDL5589830.1"
                     /translation="MSVKTEVLFSNHWNVRVSDPGFEGSPSHYFETVYLTLEAFINGE
                     NVHYEFTRKVEQEVKTHRSFTDVNELFEFLGEYLSPVALGNVGVRIGQLGLA"
BASE COUNT         1149 a          841 c          736 g         1117 t
ORIGIN      
        1 cacagtatat ctccaaatat taaagttaat caaaactcaa tacatttagg taatatacct
       61 taacaaatat aaaaaatata cctcagactg cattgtctat tttaaaagga tcaatatttc
      121 tccttaaaga gttttttgca aaaaatttgc caaatttgac cgctttttta cattaaggca
      181 ttgatttatt cgctgttttt gtttaatgta tgccgatttt attttcccta atttcaaaca
      241 acgaggcatt attatggcag acagtttcag cgttacgcgc cgcttttttg acgataaaaa
      301 ttatccacgt ggtttctctc gccacggcga ttacaccatt cgtgaatcac aaactttaga
      361 acaattcggt caagcttgct tggctttaga aagtggtgaa cgtaagccgg caactcaaga
      421 agaaaaacgt ttcgttgcgg taatgaaagg cgaagaggta gcagaaactg acattgaaaa
      481 agcgtggtta aaataccgca cattaactag taaaacaaaa cgcatctaca ccctttcagg
      541 caatgcaggt agcgatgccg gcgatgactt cagtgctgct gaataatttc taatttaaaa
      601 ccacgaagta attcgtggtt tgttttttca tttgctattt gtggtaaaaa atgactcttc
      661 ctatcgaaca atattccgat cttcttgcaa aaaaaaccga aaatttgacc gctttactca
      721 agccgtttaa cccgcctgcg attgaggtat ttgcctcaaa gccaagccat ttccgtatgc
      781 gggcagaatt tcgagtgtgg cacgaggaag gcgagctgta ccacattatg ttcaaccctg
      841 aaaccaaagc tcgttatcgt gtggatagct tccctattgc cagtgagctg attaaccata
      901 tgatgaccgc attattggcg gaaattaaag gcaatgaact actgactcgc aagctgttcc
      961 agatcgatta tttaagcacc ttaagcggtg aaattgcagt ctcaatgctt taccacaaaa
     1021 gcctgaatga agaatgggtt gaacaggcaa acgccttaaa aagccgttta gagcaacaag
     1081 gtttcaaagt gcaaattatc gggcgtgcca ataaacaaaa aatcgcactg gattgcgatt
     1141 atgtggaaga aattctaccg gtacacggca aaaattatgt gtatcgccaa gtggaaaaca
     1201 gctttaccca gccgaatgcg gaaatgaata ttaaaatgct ggaatgggcg agaaattgca
     1261 caaaaaatag tgaaggcgat ttgcttgagc tttactgcgg caacggcaat ttttcgattg
     1321 ccttggcgga aaacttccgc aaagtgctgg caaccgagat ctcaaaatct tcagtgcaat
     1381 cggctcaata caatattgaa aaaaatggca ttgttaacct acaaattatc cgaatgtcgg
     1441 cagaagaatt tacccaagcg atgaacggtg tgcgagcctt ttatcgctta aaagggattg
     1501 atttaaacgc ctacgaatgc aataccattt ttgttgatcc acctcgtgca gggttagatc
     1561 aagctacatt aaatatggtg caagcgtatg agcggattct ctatatctct tgcaatccgg
     1621 aaacccttgc cgagaattta caacaattaa gccaaaccca tcggattgaa aaagccgctc
     1681 tttttgacca attcccttac acccaccata ttgaaagtgg cgtttggtta attagaaaat
     1741 aaattgtgct gaaaaaaaga acgaataagc agcttttaag atttattact tgacgatgtt
     1801 cgcaataatc tctattattg caacgttttc aacggaggaa tggcagagcg gttgaatgca
     1861 ccggtcttga aaaccggcga gggttaacgc cctccgtgag ttcgaatctc acttcctccg
     1921 ccagtcttta aacctgttga gcaatcagca ggttttttta tttcatttgc aagaaaatca
     1981 gataaaacca ccgcttgaat ccatcgctat aaactggttg aaatcagcct aaagttgctt
     2041 taggataatt gcgattcatt atcaataact taattattat gaaatacctt tatattatct
     2101 gtggttttat ttgtattggt ttaggcatca tcggggcagt attaccgggc ttgccgacca
     2161 caccgttctt acttttggct ctgttctgct ttactaaagg ctcggagaga atccgccagt
     2221 ggtttattca aaccaaatta tacaaaaaat atctgcaaga atatgatgag aaacgggcaa
     2281 tgacgatgaa gcaaaaaatc acgattttag cgatttcgtt tccgtttaca ctatttgctt
     2341 tctttgcact gccccatatc ggtgggaaaa ttgcgttagt agtgttagtg ataattcaat
     2401 attggtattt cttctgcaaa atgaaaaccc ttgaaccgga gaatgcctaa tcttcctgct
     2461 tccgccttgc tcttgggtgg gcagagtcat aaattttagc taagtgttgg aaatccagat
     2521 gcgtataaat ttgagtggtg gacaggctac tatgacccaa taattcctga accgctcgca
     2581 aatcaccgct tgcctctaac atatgagtcg caaaagagtg acgtaattta tgtggatgca
     2641 gatggctttc cagcccttgt cgctcacccc atttcttcat tgccaactga atggaacgat
     2701 gagataaccg cccacctcgc ttattcagaa acaccgcatt atcttgcggc ttaaattgca
     2761 aacgcatttc taaccagcga tttaacgctt ttaacgcttg cgagccaatc ggcacaattc
     2821 gctctttgct acccttacct tgcacccgta cttctcgtgc cgataaatcc atatcgccta
     2881 aatccagccc ttgtaattcc gataaacgca agccggaact atacatcagc tccatcatcg
     2941 caatatctcg aatatcgctc ggctcatcac tctcaatcgc cagcaactgc ccgacctgct
     3001 ccgcatcaat atttttcggc aaatgcttac ctactttcgg cgatttcacc cctaaagtcg
     3061 ggtttgccga cattcgcccg attttaatca aatagacaaa ccattgccgt aacgccacca
     3121 agcgtaagcc gatacttttc gcactcaacc cttgcttatg gctttggctt aacatccaac
     3181 gcaccgagct actttcgacc tcttcccaat tttcaatgcc tgcttgcgaa aaaagctcgc
     3241 tcaccgccat taattggcgt tgataattgc ttaaggtatg cgggctaact tgcttttcaa
     3301 tgcgtaagaa atcccaatag gggcgggttt gttggaaaag agggttggaa gtggtcataa
     3361 acgtaataac aagcggttaa tttttaggaa aatgttgcaa gggcaaaaga tttgcccttt
     3421 gagattaggc tagccccaac tgaccgatac gcacgccaac attaccaagt gcaacagggc
     3481 ttaaatattc gccgagaaat tcaaacaatt cattcacatc agtaaaactg cgatgagttt
     3541 tcacttcttg ctccacttta cgagtaaatt cataatgtac attttcgccg ttaataaagg
     3601 cttccagcgt taaatatacg gtttcaaaat agtggcttgg cgagccttca aagcccggat
     3661 cactcactcg aacattccaa tgattactaa ataaaacttc ggtttttact gacattgtgt
     3721 tttccttctt cctataagta cgtacattat tttaatcaag atagattgaa tttgccactt
     3781 ttcctagtca gaatcgaggc aaatcttatt tggtttgtcc tcttatatct ttccctcaaa
     3841 ata
//
LOCUS       DANLZC010000181         3718 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.181, whole genome shotgun sequence.
ACCESSION   DANLZC010000181 DANLZC010000000
VERSION     DANLZC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.181"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(100..1602)
                     /gene="ppx"
                     /locus_tag="PXE44_002372"
     CDS             complement(100..1602)
                     /gene="ppx"
                     /locus_tag="PXE44_002372"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDL5589831.1"
                     /translation="MSSNIAPREFAAIDLGSNSFHMIVARIVNGSIQVLSRLKRRVRL
                     ADGLDENLVLSQDAIDRGIACLALFAKRLEGFSAENVKVVGTYTLRRAINNQAFLEQA
                     KAVFPYPISIISGQQEARLIYSGVSHTQPETGRKLVVDIGGGSTEMTIGDDFTPIRAE
                     SRHMGCVSFAKRFFPQGELTIERFDSAYQLAMEKIEDLMWEYKELGWDYALGSSGTIK
                     TVHQVLIANGYRDGLITESRLQKIIDICLEFKSLNEIKLRGLLDERADVLVPGLAILL
                     ALFHTFRIDVLRYSNGALREGVMYGLEKSFQVGDIRQRTAEALAEQFALDKQQAKRVE
                     HTALILFDQIKTWKNRRQVQDLRSMLKWASLLHEIGIVVNHNNVHRHSAYLIANRDLP
                     GFDFEQQTLLSVLMRYHLKGFKRSDIRSINRYQCRDVLTLVRVFRLAVLLNRSRQATV
                     VPKQLNLVIENNAWQLTFEQGFLANNPLISIDLEEEQKILASTNLQFAVS"
     gene            1798..2127
                     /locus_tag="PXE44_002373"
     CDS             1798..2127
                     /locus_tag="PXE44_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDL5589832.1"
                     /translation="MSEVNKSFHDMLEYVHLYRLKNKLHRETADNDRKIRDNQKRVLL
                     LDNLTQYITDEMSIEEVRAIISNMRDDYENRVDDYMIRNAELSKQRREIRQKMASHKQ
                     APTNKKA"
     gene            complement(2191..3621)
                     /locus_tag="PXE44_002374"
     CDS             complement(2191..3621)
                     /locus_tag="PXE44_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDL5589833.1"
                     /translation="MSTLTYLQKIGRALMVPVAALPAAALLMGIGYWIDPDGWGTNSQ
                     LAALLIKSGAAIIDNMSILFAVGVAFGMSKEKDGAAALSGLVGYLVITTLLSPGTVAQ
                     LKGIAVDQVPAAFGKINNQFIGILVGVISAEIYNRFYQVELPKALSFFSGKRLVPIVV
                     SFILLYVWPVIFGALVSFGESIKDMGALGAGIYAFFNRLLIPVGLHHALNSVFWFDVA
                     GINDIPNFLGGAKSLAEGKAVIGVTGMYQAGFFPMMMFGLPAAALAIYHSAKPAKKMQ
                     VASIMFAAALASFFTGITEPLEFAFMFVAPVLYVVHALLTGLSVYIAASMEWIAGFGF
                     SAGLVDLVLSTRNPLAVQWYMLLAQGIVFAVIYYVLFRVVIQAFNLKTPGREEDSEVQ
                     TVNTVSRSEQAAKFVEALGGKENITSIDACITRLRLTLNDTAKADETKLKALGSKGNI
                     KLGENGYQVILGPEAEFVADAMKKLN"
BASE COUNT         1205 a          851 c          647 g         1015 t
ORIGIN      
        1 aaaatacttt ttgcaaaaaa ttagctaaat ttgaccgctt gttcgcaaaa gggaatgcaa
       61 taaacgaaaa aggaagcctt cgcttccttc atttaaatgt taagagaccg caaactgaag
      121 atttgtactc gccaatattt tttgctcttc ttctaaatct attgaaatca gtggattatt
      181 cgctaagaag ccctgttcaa aggtaagttg ccaagcatta ttttcaatca ccaaattcaa
      241 ctgtttcggc actacagtcg cttggcgaga acggtttaat agcaccgcta gccggaaaac
      301 ccgtaccaac gttaatacat ctctacattg gtaacggttg atactacgaa tatccgagcg
      361 tttgaatcct ttaaggtggt atcgcattaa taccgataga agggtttgct gttcaaaatc
      421 aaatcccggt aaatctcggt ttgcaatcaa ataagcagaa tgacgatgca cattattgtg
      481 gtttacaaca ataccaatct cgtgtaataa acttgcccat ttcagcattg aacgcagatc
      541 ttgcacttgg cggcggttct tccaagtttt gatttggtca aataaaatca acgccgtatg
      601 ctccaccctt tttgcttgct gtttatctaa agcaaattgc tcagctaacg cttctgcagt
      661 ccgttggcga atatccccaa cttggaaact tttctccaaa ccatacatta ccccttctct
      721 taaagccccg ttggaataac gtaatacatc aattctaaaa gtgtgaaaaa gtgctaaaag
      781 aatagctaag cccggaacta gcacatctgc tcgttcatca agtaagcctc gtagcttaat
      841 ctcatttaac gatttaaact ctaggcaaat atcaataatt ttctgtaggc ggctttctgt
      901 aattaagcca tcacgatagc cattggcaat cagcacttga tggacggttt taatcgtacc
      961 ggaagaacct aaagcatagt cccaacctaa ctctttgtac tcccacatta aatcttcgat
     1021 tttttccatt gcaagctgat aggcactatc aaaacgctca atcgttaatt ccccctgcgg
     1081 gaaaaagcgt tttgcaaaac tcacacaacc catatgacga ctttccgctc gaatcggggt
     1141 aaagtcatca ccaatagtca tttccgttga accaccgcca atatctacca ctaattttct
     1201 gccagtttcg ggttgggtat ggctaacgcc cgaataaatc agcctcgcct cttgttgtcc
     1261 ggaaataatg gaaatcgggt aaggaaatac tgctttcgct tgctccagaa aagcttggtt
     1321 attgattgct ctacgtaagg tataagtgcc aaccaccttc acattctctg cagagaaccc
     1381 ttctaaacgt tttgcaaata aagctaaaca agcaatgcct ctatcaatcg catcttggct
     1441 taatactaag ttttcatcaa gcccatctgc cagccgtaca cgccgtttca aacgggaaag
     1501 cacttgaatg gaaccattta cgatacgggc tacaatcata tgaaagctgt tcgagcctaa
     1561 atcaatagct gcaaactctc taggagcaat atttgatgac ataatgaaac cctgtaccaa
     1621 taactgaaat aagtaagata ttaccatttt tgagccctag atatcactac aaatttagcg
     1681 ttattcgcga gaattaattt aaatcataaa atgtgataaa tttttttgac gaattgacgg
     1741 aaaaagaata ggatttacga gcttacttaa ttcaacacta catcaaggga gattgatatg
     1801 tctgaagtga ataaatcatt ccacgatatg ctggaatatg tgcatcttta tcgtctaaaa
     1861 aacaaactgc accgtgaaac ggcggataat gatcgcaaaa ttcgagataa tcagaagcgt
     1921 gtacttttat tagataacct aacgcaatat attaccgacg aaatgtctat cgaggaagtt
     1981 cgagcgatta tttccaatat gcgtgatgac tatgaaaatc gagtagatga ttatatgatc
     2041 cgtaatgcgg aactttccaa acaacgccgt gaaatccgcc aaaaaatggc ctctcacaaa
     2101 caagcaccaa ccaataaaaa agcataaaaa gccaagcggt ctgatttgca aaaaaatatg
     2161 caaatcagac cgcttgtgat taagcagaga ttaattcagc tttttcattg catctgccac
     2221 aaattctgct tccggtccca aaatcacttg gtagccattc tcgcctaatt tgatattacc
     2281 ttttgaccct aacgctttca atttggtttc gtctgctttc gccgtatcat ttaaggttag
     2341 gcgtaagcgg gtaatacaag catcaatgct ggtaatattc tctttaccac ctaatgcttc
     2401 aacaaatttg gcagcttgtt cgctacgaga aacagtattc actgtttgta cttcgctatc
     2461 ttcctcacgc cccggcgttt ttaaattaaa ggcttggatc actactcgga acaatacata
     2521 gtaaattact gcgaatacga taccttgggc caataacata taccactgca ctgccagcgg
     2581 atttcgtgtc gataacacta aatcgaccaa ccctgcacta aaaccgaaac cggcaatcca
     2641 ttccattgag gctgcaatat aaacagataa tccggttaat aatgcgtgaa ccacataaag
     2701 cacaggtgca acaaacataa aggcaaattc aagcggttcc gtaatgccgg tgaagaacga
     2761 agctaaagca gcagcaaaca taatagaggc tacttgcatt ttcttagccg gtttcgcact
     2821 gtggtaaata gctaatgctg ccgcaggtaa gccaaacatc atcatcggga agaaaccagc
     2881 ttgatacata cctgttactc ctatcactgc tttgccctca gccaatgatt ttgccccacc
     2941 taagaagttt ggaatatcat taatgcctgc aacatcaaac cagaaaaccg agtttaatgc
     3001 gtggtgtaat ccaaccggaa ttaataaacg attaaagaaa gcataaatac ctgcccctaa
     3061 cgcccccata tctttgatag attccccaaa agagactaaa gcaccaaaaa taaccggcca
     3121 gacataaagc aagataaatg acaccacaat cggcaccaat cgtttaccgc taaagaaaga
     3181 caatgctttc ggtaactcta cttggtagaa acggttataa atttcggctg aaataacccc
     3241 tactaagatc ccgataaatt ggttgttgat tttaccgaat gctgcaggca cttgatcaac
     3301 tgcgatccct tttagctgag caacggtccc cggagacaac aaagtggtaa ttactaaata
     3361 cccgactaaa ccggataatg ctgctgcccc atctttttct tttgacatac caaatgccac
     3421 acccacggca aatagaatag acatattatc aataattgcc gccccagatt taatcagcaa
     3481 tgccgctaat tggctatttg ttccccagcc gtccggatca atccaatagc ctatacccat
     3541 caataatgct gctgctggta atgcagcaac cggcaccatt aatgcacgcc caattttttg
     3601 taaataggtt aatgtactca taaaagcctc caaaaatacc tcataatgag taacttaagc
     3661 atagaaaaaa accgattaaa gaacaatcaa ataaatttgg ttttgtgatc catttcaa
//
LOCUS       DANLZC010000182         3683 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.182, whole genome shotgun sequence.
ACCESSION   DANLZC010000182 DANLZC010000000
VERSION     DANLZC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.182"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(137..2701)
                     /locus_tag="PXE44_002375"
     CDS             complement(137..2701)
                     /locus_tag="PXE44_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HDL5589834.1"
                     /translation="MRIAKIIFSALLSLLVLGGVIGGLLYMHFKSDLPDVATLKNVEL
                     QQPMQIFTLDGKLIGEVGEERRIPVKLDDIPPMLVKAILATEDARFYEHKGIDPKGIM
                     RAIWRTSQGDTQGASTITQQLAKNFFLTPERSLERKAKEAILALEIEQVLSKNEILEL
                     YLNKIYLGYRSHGVAAAAKTYFNKSLKDLTLSEIAIIAGLPKAPSTMNPLYSVKRAED
                     RRNVVLGRMLETGDITKEEYEKAKAEPVVANYYGATLEFRADYVTEMVRQEIVKRYGE
                     NIAYNKGLKVYATVLSADQKAAQDALRENLIDYDRRHGWRGAEKLWTGSSAWDDEKII
                     DHLSKLPKSEPFTPAVVMQADKSKYTILLANGDISVLKRTNASFAPKGLKAGEQIWVR
                     QNKNREWALGQIPEVNSALVSINSENGAIESIVGGFSFEQSRFNRATQSLVQVGSAIK
                     PFIYTAAMNKGLSLSTTISDSPIVIKKKGQKEWRPKNADGVYGGPLRTRVALGKSKNM
                     VAIRILQMAGIDYVADYLQRFGFNRNQYVATESLALGAASFTPLEMARAYAVFNNGGY
                     LIEPYIIDRIIDAQGYELYQANPVVACNSCDSPVVYPEPKYFESVKIHDEAQVGVQTI
                     ANSSNNDDDDVVEDVGDSEPELQANNSEKNAPSLMAESSHHIKGLRYAPHVISDELAF
                     LMRSALATAITGEPEYGWRGTSYKMLNTIKRADVGGKTGTTNNAKATWYAGFGANIST
                     VVYVGFDDNKRVLGRGASGSSTALPAWTNYMKVALSDKPVEKSTLPKNIIEVNIDPNS
                     GFLGNGRKEYFIKGTEPTKRYIVERAYTEKAPQKTESAPLRLGLPPPGVLKSGELF"
     gene            2825..3523
                     /locus_tag="PXE44_002376"
     CDS             2825..3523
                     /locus_tag="PXE44_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HDL5589835.1"
                     /translation="MKLLKAHSKTPSNRIILGLSEDEQRYCLVKNKAEKFTVFWQAKP
                     ASIEELIGNLTPQEQAMMTLVRPIPYQYIWRKTVFMANTLNEAQLHRKVIHILKNEQP
                     LALDLLNIDYQRFPSSNDNLDKVVIYAVRKSYTENLSQFNSILDCELHCYARAIIHLT
                     QSQNNQPAPCFSFKQKYFQFTETELLILQTEPEDCIHLSDLKIDHLEIECEQEKQLYC
                     LALGASLWNGKVLI"
     gene            3499..>3683
                     /locus_tag="PXE44_002377"
     CDS             3499..>3683
                     /locus_tag="PXE44_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589836.1"
                     /translation="MEWQGIDLSANRKNNWFAGSDKGIKNVIYTLLLSALISLALIFL
                     YLQKNQEIQPLAQQLELV"
BASE COUNT         1118 a          816 c          612 g         1137 t
ORIGIN      
        1 tgcaataaaa ctcatttatt gactaaggaa tatccaaata taactcacaa atcgcccaaa
       61 ataaaattgg cacttattat cgcaagaaat ccccctattt ttcaactttt aagagcatta
      121 tccttgtaat aaattattag aataattcac cgctttttag cacccctggt ggaggtaatc
      181 ctaaacgtaa cggcgcagat tctgtttttt gaggcgcttt ttcagtataa gctctctcta
      241 caatataacg ttttgtaggc tcagtaccct taataaaata ctctttacgt ccatttccta
      301 agaaaccact attagggtca atattaacct caataatatt ttttggaagg gtactttttt
      361 cgacaggctt atctgacaat gcaactttca tataatttgt ccaagctggt aaagcagtac
      421 tagaacctga agcacctcta cctaatactc ttttattatc atcaaagcca acgtacacaa
      481 cagtagaaat attagcacca aatcccgcat accaagttgc cttggcatta ttggttgtac
      541 cagtcttccc gcccacatcc gctcgtttta tcgtattgag cattttatag cttgttcctc
      601 gccagccata ttcaggctca ccagtaattg ctgtagctaa agcactacgc attaaaaaag
      661 cgagttcatc actaataaca tgaggagcgt aacgtaaccc tttgatatga tgagaagatt
      721 ctgccattaa gctcggcgca tttttctcgc tattgttcgc ttgtaattca ggttctgaat
      781 cgccaacatc ttccacaaca tcgtcgtcat cattattcga ggaattcgct atagtttgta
      841 caccgacttg agcttcatca tgaattttga ctgattcaaa atatttaggc tcagggtaaa
      901 caactgggct atcgcaacta ttacaagcta ctacaggatt tgcttgatat aactcgtagc
      961 cttgggcatc aataatacgg tcaatgatat atggttcaat caaataacct ccattattaa
     1021 atactgcata agctcgagcc atttctaatg gcgtaaaaga tgcagcaccc aatgcaagag
     1081 attctgttgc aacatactga tttcggttaa agccaaaacg ttgtaagtag tctgcaacat
     1141 aatcaatccc tgccatttgt aatatacgaa tagccaccat atttttcgat ttacctaatg
     1201 caacacgagt tctcaacggg ccgccataaa ccccatcggc atttttaggt cgccactctt
     1261 tttgcccttt ctttttaatg acaataggag aatcgctaat tgttgttgaa agacttagcc
     1321 ctttattcat tgccgctgtg taaataaacg gtttaatcgc agagcctacc tgaactaatg
     1381 actgagtcgc acgattgaaa cggctttgtt caaagctaaa gcctccaacg atagattcaa
     1441 tggcaccgtt ttcactatta attgacacta gggctgaatt tacctccgga atttgtccta
     1501 aagcccactc cctattttta ttttgacgta cccaaatttg ttcacctgct tttaaccctt
     1561 taggtgcaaa agaagcattt gtacgtttta aaacagagat atcaccattt gccaataaaa
     1621 ttgtatattt tgatttatct gcttgcatta caacagctgg tgtaaacggc tctgattttg
     1681 gcagcttact taaatgatcg ataattttct catcgtccca agcagaacta cctgtccata
     1741 atttttcagc gcctcgccaa ccatgacggc ggtcataatc aatcaaattt tcacgtaacg
     1801 catcttgtgc tgctttttgg tctgctgaaa gtaccgtggc gtacactttt aagcctttgt
     1861 tataagcaat attttctcca tagcgtttca caatttcttg acgaaccatt tctgtcacat
     1921 aatccgctcg aaattctaat gtcgcaccat agtagttagc tactactggc tctgctttag
     1981 ctttctcata ttcctctttt gtaatatctc cggtttctag cattctccca agcactacat
     2041 tacgacggtc ttccgcccgt tttactgaat aaagtggatt catcgttgaa ggcgcttttg
     2101 gaagccctgc aataattgct atttcagaaa gggttaaatc tttcaaagat ttattaaaat
     2161 aagttttagc cgcagcggca acaccgtgag aacgataccc taggtaaatt ttgtttaaat
     2221 acagctccag aatctcattt ttactgagaa cctgttcaat ttctaaagct aaaattgcct
     2281 ctttggcctt acgctctaaa ctacgttcag gtgttaagaa aaagtttttc gccagctgct
     2341 gggtgattgt acttgcgcct tgagtatctc cctggctagt gcgccatatt gcacgcataa
     2401 tacctttagg atcgatccct ttatgctcgt aaaaacgtgc atcttcggta gcaagaatcg
     2461 cttttactaa catcggtgga atatcatcaa gcttgaccgg aatacggcgt tcttctccta
     2521 cttcaccaat taatttacca tctaaggtaa aaatctgcat aggctgctgt aactctacat
     2581 ttttcagcgt tgccacatca ggcaaatcag atttgaaatg catgtacagt aaaccaccaa
     2641 ttaccccacc taaaaccaag agtgaaagta aagcactaaa tattattttt gcgatccgca
     2701 tcgaaaaaat ctcttttggt taatgacgaa acataaattt aaagccaata ttaggtcact
     2761 aagtataagc taaaaacgct tacttcgcat aactaattgc aaaactttcc ttactatact
     2821 tactatgaaa ttacttaaag cacattcaaa aacaccttca aaccgtatca tcttaggctt
     2881 aagtgaagat gaacaacgtt actgcttagt caaaaataaa gcagaaaaat tcaccgtttt
     2941 ttggcaagcc aagcctgcga gcatcgaaga gcttatcggt aatctcaccc cacaagaaca
     3001 agcaatgatg acgctggttc gcccgatccc ttaccaatac atttggcgaa aaaccgtttt
     3061 tatggctaac accctcaatg aagctcaatt acaccgtaag gtaattcaca ttttaaaaaa
     3121 tgagcagcca cttgcattgg atctactcaa tattgactat cagcgatttc caagcagcaa
     3181 cgataatctg gataaagtgg ttatctacgc cgtgcgaaaa agttataccg agaatttgag
     3241 ccaatttaac agcatcttag attgtgagct gcattgctat gcacgggcta taattcattt
     3301 aacacaatcc caaaataatc agcctgcgcc ctgtttttca tttaaacaga agtacttcca
     3361 atttaccgag acggaattac tgatattgca aacagaacct gaggattgta ttcatctatc
     3421 tgatctcaaa attgaccatc ttgaaatcga gtgtgagcaa gagaaacagc tatattgcct
     3481 cgccttagga gcaagtttat ggaatggcaa ggtattgatt taagtgctaa tcgaaaaaat
     3541 aactggtttg ccggcagtga taaaggcatc aaaaacgtga tttatacact tttattatct
     3601 gccctcatta gcttggctct gatttttctc tatttgcaaa aaaatcaaga aattcaaccg
     3661 cttgcacagc aattagagct ggt
//
LOCUS       DANLZC010000183         3669 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.183, whole genome shotgun sequence.
ACCESSION   DANLZC010000183 DANLZC010000000
VERSION     DANLZC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.183"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            65..2041
                     /gene="cpdB"
                     /locus_tag="PXE44_002378"
     CDS             65..2041
                     /gene="cpdB"
                     /locus_tag="PXE44_002378"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDL5589837.1"
                     /translation="MLNRRKFIQLGATAMLVLGTQPSAFAQTAKNKAGLRVIATTDIH
                     GFLTDFDYYKDAPTEKFGFTRTASLIKQARAEVKNSVLVDNGDLIQGNPISDYQSTKG
                     AKEGKPEPSIMALNAMQYEVGTLGNHEFNYGLSYLDNAIKQAKFPIVNANIVKPGTDE
                     PFFTPYVIQQKEIVDEKGNKHTIKIGYIGFVPPQVMIWDKANLEGKVEARDIVKTAQK
                     YVPIVKKEGADIVVALAHTGPSDEPYKEGAENCAFYLADVKGIDAVIFGHSHRLFPNK
                     EFANSPNADIAKGTMKGVPESMAGYWGNNISVIDLSLAENDGKWGVVDGRAELRPIYN
                     TEEKKATVENHPEIVALLKETHEATRQFVSQPIGKATDNMYSYLALVQDDPTIQIVNQ
                     AQKAYVENVVKGLPELAGLPILSAGAPFKAGGRKNDPTGYTEVDKGELTFRNAADLYL
                     YPNTLVVVKATGAELKEWLECSAGMFKQIDVASDKPQYLLDWTGFRTYNYDVIDGVSY
                     QFDITQPARYDGECKLINPNSHRVVNLTFDGKPVDPKQEFLIATNNYRAYGAKFPGTG
                     DKHIVFAAPDENRQVLANYITTESKAHGQVSPKADNNWRIAPISSNAKLDIRFETSPT
                     DKAKAFIKENAQYPMEYIENDETGFAVYRIDLTK"
     gene            complement(2114..3592)
                     /locus_tag="PXE44_002379"
     CDS             complement(2114..3592)
                     /locus_tag="PXE44_002379"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL5589838.1"
                     /translation="MDSILGKKINSTAIPSLLCDFYKTSHRIQYPEGSQFLYSTFTPR
                     SNIYAPFVTRVVSFGFQAFILKYLIHYFNDNFFSRSQHDVVSEYTQFISDTLNIEDSG
                     EHIAQLHQLGYLPIRIKAIPEGKSVAVKVPLMTIENTHSSFFWLTNYLETLINVSLWQ
                     PITSASIAFAYRKALAEFSEQTCDDNSHLPFQAHDFSMRGMSSLESAESSGAGHLTSF
                     LGTDTIPAISFVTAYYGSQHLIGTSIPASEHSVMSSHGVDELPTFRYLMAKYPNNMLS
                     IVADTTDFWHNITVNLPLLKQEILARPESAKLVIRPDSGDFFTIICGNNSSPVEHERK
                     GLIECLWDIFGGSINQKGYKVLNPHIGAIYGDGVTYDKMIRILEGLTAKGFASSNIVF
                     GVGAQTYQRNTRDTLGFAIKATSITINGVEKAIFKAPKTDNGLKKSQKGRVKVISYDH
                     YVDELTALDDFSDDLLEVVFENGKLVKSTNFDEIRQNIAQQF"
BASE COUNT         1229 a          685 c          727 g         1028 t
ORIGIN      
        1 acaagcggtt atttttctaa aatattttac aatttaccct ttcatttcat taaggagata
       61 ttttatgtta aatagaagaa aatttatcca gcttggtgca actgcaatgc ttgtattggg
      121 gacacaaccg tctgcatttg cacaaacagc caaaaataaa gctggcttgc gtgtcatcgc
      181 cactacggat attcacggtt tcttaactga ttttgattat tacaaagatg ctccaacaga
      241 aaagtttggt tttacccgta cggcaagcct aattaaacaa gctagagcag aagttaaaaa
      301 cagtgtgtta gtcgataatg gtgacttaat tcaaggtaat cctatttctg actaccagtc
      361 aaccaaaggt gcaaaagagg gcaaacctga accttcgatt atggcattaa atgcgatgca
      421 atatgaagtc ggaacgctgg gaaatcatga atttaactac ggtttaagct atttagataa
      481 tgctattaag caagcaaaat tccctattgt caatgcaaac attgtgaagc cggggacaga
      541 tgaaccattc tttacacctt atgttattca acaaaaagaa attgtagatg aaaaagggaa
      601 taagcatacc attaagattg gttatatcgg ttttgtacca ccacaagtga tgatttggga
      661 taaagctaat cttgaaggaa aagttgaagc tcgagatatt gtgaagaccg cacaaaaata
      721 tgtgcctata gtaaaaaaag aaggggcaga tattgtcgtt gcattggcac ataccggccc
      781 ttcagacgaa ccctataaag aaggcgctga gaactgtgcg ttttatctag ctgatgtaaa
      841 aggtattgat gcagttattt tcgggcattc acatcgcctg ttcccaaata aagaatttgc
      901 aaattcacca aatgccgata ttgcaaaagg aacaatgaaa ggcgtaccgg aaagtatggc
      961 tggctactgg ggaaataaca tcagtgtgat tgatttaagc ttggccgaaa acgatggtaa
     1021 atggggcgtt gtggacggta gagcagagtt gcgtccaatt tataatacgg aagagaaaaa
     1081 agcaaccgtt gagaaccacc ctgaaatcgt tgcattatta aaagaaaccc atgaagcaac
     1141 ccgtcaattt gtgtctcaac ctatcggtaa agcaactgat aatatgtata gttaccttgc
     1201 gttagttcaa gatgacccta ccattcaaat tgtgaaccaa gctcaaaaag cgtatgtaga
     1261 aaatgtggta aaaggcttgc cggagcttgc aggattacca attctaagtg ccggagcacc
     1321 gtttaaagcc ggtggacgta aaaatgaccc gacaggctat accgaagtcg ataaaggcga
     1381 attaactttc cgcaatgcgg cagacttata cctttaccca aataccttag tggtggttaa
     1441 agcaacagga gcagaattaa aagagtggtt agagtgcagt gcaggaatgt tcaaacaaat
     1501 tgatgtcgct tcggataaac cgcaatattt actggattgg acaggtttcc gcacctataa
     1561 ctacgatgta attgacggtg tcagctatca atttgatata acccaaccgg ctcgttatga
     1621 cggcgagtgc aaattgatca accctaattc gcaccgtgtg gtgaacttaa cctttgacgg
     1681 caaaccggtt gatcctaaac aagaattctt aattgccacc aacaattacc gtgcttatgg
     1741 agcaaaattc ccgggaacag gcgataaaca tattgtgttc gccgcaccgg acgaaaaccg
     1801 ccaagtgtta gcgaattata ttaccaccga aagtaaagct cacgggcagg taagtccgaa
     1861 agcggataac aactggcgta ttgcaccaat tagctcaaat gcaaaactgg atattcgttt
     1921 tgaaacctct ccaacagata aagctaaagc tttcattaag gaaaatgcac aatatccgat
     1981 ggagtatatc gaaaacgatg aaaccggctt cgcagtttac cgtattgatt taaccaaata
     2041 atcagagcta aatacaagcg gtctaatttg caaaaaaatt aacaaatttg accgcttgtt
     2101 tcattccatt tttctaaaat tgttgtgcta tattttgccg aatttcatca aaattggtcg
     2161 atttgaccaa cttaccattt tcaaaaacca cctctaaaag atcatctgaa aaatcatcca
     2221 gtgcggttag ctcgtcaaca taatgatcat aagagattac ttttactcgc cctttttgcg
     2281 atttttttaa gccgttatcg gttttaggag ctttgaaaat tgccttttcg acaccgttaa
     2341 ttgtaattga agtcgctttg attgcaaaac ccagcgtatc acgagtattg cgttgataag
     2401 tttgagcccc aacgccaaac acaatattgc tggaagcaaa accttttgcc gttaagccct
     2461 ctaaaatccg tatcatttta tcataagtga cgccgtcacc ataaattgcc ccaatatgcg
     2521 gattaagcac tttataacct ttttggttaa tactaccacc aaaaatatcc cacaaacact
     2581 caatcagacc tttgcgttcg tgttcaacgg gggaggaatt gttaccgcaa ataattgtaa
     2641 aaaaatcacc gctatcaggg cggataacca attttgctga ttccggtcga gctaaaattt
     2701 cttgttttaa taatggcaaa ttaacggtga tgttatgcca gaaatcggtt gtatctgcca
     2761 caatagatag catattattt ggatattttg ccattaaata acggaaagtc ggcagctcat
     2821 caacaccgtg tgaactcatc accgaatgtt ctgatgccgg aatagatgta ccgattaaat
     2881 gttgagaacc ataataggct gtcacgaatg aaattgcagg aatggtatct gtgcctaaaa
     2941 atgaggttag atgtccggca ccggagcttt ctgctgattc taaggaactc attccacgca
     3001 tagagaaatc gtgtgcctgg aagggtagat ggctattatc atcacaggtt tgctctgaaa
     3061 attcggctag agccttacga taagcaaatg caatggaagc tgatgtaata ggctgccata
     3121 atgacacatt aatcagggtt tccaaataat tagtcagcca aaaaaagctt gagtgagtgt
     3181 tttcaattgt cattaaagga actttcactg caacagattt tccttctgga atagctttaa
     3241 ttctgatagg taagtaaccg agctgatgca attgtgcaat atgttcgcca gagtcctcaa
     3301 tattcaatgt gtcagaaata aattgagtat attcagaaac aacgtcatgt tgtgagcgag
     3361 aaaaaaaatt atcgttaaaa taatgaatca agtattttaa aataaatgcc tgaaagccaa
     3421 atgatacaac acgtgtaaca aatggagcat aaatattact acgaggtgtg aatgtactat
     3481 ataaaaactg agaaccttca ggatattgaa ttcgatgtga agttttataa aaatcacata
     3541 atagtgatgg tattgcagtt gaattaattt ttttacccaa aatactatcc attttcttaa
     3601 agtccttatt taacttttaa ggctaaaggt tatcataact taccataaaa tgaaaagtat
     3661 ttgttacaa
//
LOCUS       DANLZC010000184         3663 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.184, whole genome shotgun sequence.
ACCESSION   DANLZC010000184 DANLZC010000000
VERSION     DANLZC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.184"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..1187)
                     /locus_tag="PXE44_002380"
     CDS             complement(<1..1187)
                     /locus_tag="PXE44_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3880 domain-containing protein"
                     /protein_id="HDL5589839.1"
                     /translation="MKKKQQYRKSTINNTNASQQNSGEVLNSVNYQTLLEDYLNKISF
                     SNRQFIDELKTEHNKKLKELENKVSSLEQTNHVQQQDISRLNILLEQKNIEENRNLER
                     MISYQLGYALINACKSFSGFISLPSELLKIRKLAKDKKQQKLLPSSPVANTPKQQTAL
                     VTSAPARSQSIDLTRGLTLLDPISELCWADAFTGYPLVRKSYADHIAGSTCKFAFFES
                     AWQANKGSWIYAFTSPGLKHNNAQALLDAIQKLKDKSIPIIFWNKEDPMHYEMFKPIA
                     EKVDYVFTTDQLVVDKYKKELGHSNIWALPFAAPIKVTNPIGRFSLDTETVCFAGTYY
                     AQNHENRKKQMDMLLPALLAYNGCIYDRASKDTSGKYAYPEQYTHLIREGVNFNEIVK
                     LYK"
     gene            complement(1191..2462)
                     /gene="wecC"
                     /locus_tag="PXE44_002381"
     CDS             complement(1191..2462)
                     /gene="wecC"
                     /locus_tag="PXE44_002381"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL5589840.1"
                     /translation="MSNFNTISVVGLGYIGLPTATVFAQHGLNVIGVDVNQHAVDHIN
                     QGKIHIVEPDLDVAVHQCVSSGKLKATLTPEPAEAFLIAVPTPFKGEDYEPDLSYIQA
                     ACKAIAPVLEKGNLVILESTSPVGATEQMAEWLAEYRPELSFPQQAGEDSDIRIAHCP
                     ERVLPGQVMHELIENDRIVGGMTNKCSVQAVNLYKTFVKGDCIVTNARTAEMCKLTEN
                     SFRDVNIAFANELSIICDRLDINVWELIALANRHPRVNILQPGCGVGGHCIAVDPWFI
                     VNKTPDLAKIIRTAREVNDHKPEWVISKMNEAVIYALQKTGKSIDQIKIACLGLAFKP
                     DIDDLRESPALKITEKLAEKYPNQVFAVEPNVEELPKKLANKNISLISVDEALEVADV
                     VVVLVDHSEFKLVKPVFLNDICVVDTKGIWR"
     gene            complement(2477..3592)
                     /gene="wecB"
                     /locus_tag="PXE44_002382"
     CDS             complement(2477..3592)
                     /gene="wecB"
                     /locus_tag="PXE44_002382"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDL5589841.1"
                     /translation="MRYLIVFGTRPEAIKMAPLVEGFKKSGLNFKVCVTAQHRQMLDQ
                     VLELFEITPDYDLDIMSNKQTLSTVTSAILEKIQPVIAEYKPTTIFVHGDTATTLASS
                     LAAYYNQIDIAHIEAGLRTHNIYSPWPEEGNRKLTAALAKYHFAPTQSTKKNLLNENI
                     AEGTIYVTGNTVIDALFLACKKLDENIDLLSSLQEKFSYIKSKRTILITGHRRENFGN
                     GFENICKAVSTLASDFPDVQFIYPVHLNPNVREPVNRLLADKSNVHLIEPCDYLSFVY
                     LMRESYLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVEAGTVKLVGTAQSEIIKQV
                     TELLNDQAAYTKMSEAHNPYGDGTAVKQILSVFQSKQ"
BASE COUNT         1085 a          692 c          661 g         1225 t
ORIGIN      
        1 ttcttataca acttaactat ctcattgaaa tttacccctt ctcggataag atgagtgtat
       61 tgttcaggat aagcatattt gccacttgta tctttagatg ctctatcata aatacagccg
      121 ttgtaagcca ataatgcagg taacaacata tccatctgtt ttttacgatt ttcatgattt
      181 tgagcataat atgttccagc aaaacaaact gtttctgtat caagagaaaa tctgccaatt
      241 ggattggtta ctttaattgg ggcagcaaaa ggcaaagccc aaatatttga atgcccaagt
      301 tcttttttat atttatctac aaccaattga tctgttgtaa aaacataatc tactttttct
      361 gcgattggct taaacatctc ataatgcatt ggatcttctt tattccaaaa aatgatagga
      421 atgcttttat cttttagttt ctgaattgca tctaacaatg cttgagcatt attatgtttt
      481 aagcctggtg aagtaaaagc atagatccat gaccctttat ttgcctgcca agcactttca
      541 aaaaaagcaa atttacacgt gctgccagca atatgatcag cataagattt tctaactaaa
      601 ggataccctg taaaggcatc tgcccagcat aattcagaaa taggatccag tagtgtcaat
      661 cctcttgtca agtcaataga ttgagatcgg gcaggtgcac ttgtaactaa tgcagtctgt
      721 tgctttggtg tatttgcaac aggggaagat ggcaataatt tctgctgttt cttatcctta
      781 gctaacttcc tgatttttaa taattctgaa ggcaaggata taaaaccact aaatgattta
      841 caagcgttta ttaaagcata tcctaactga taagaaatca ttctttcaag atttctattt
      901 tcttcaatgt tcttttgctc taacaaaata tttaatctgg atatgtcttg ctgttgaaca
      961 tgattagttt gctcaaggct gcttacttta ttttctagct ccttgagctt tttattatgc
     1021 tcagttttca gctcatctat aaactgccga ttactaaaac taattttatt taaataatct
     1081 tccagtaatg tttgataatt gacagaattt aatacttcgc ctgaattttg ttgtgatgca
     1141 ttagtattgt taatagtgga tttacgatat tgctgtttct ttttcatcac ttatctccaa
     1201 attcctttag tatccaccac acaaatatca ttcaaaaaca caggtttgac tagtttaaat
     1261 tcgctatgat ccaccaaaac aactacaaca tcagctactt ccagagcttc atcaacagaa
     1321 atcaaagaaa tgttcttgtt agccaatttt ttcggtagct cttcaacatt cggctcaact
     1381 gcaaatactt gatttggata cttttccgcc aatttttcag taatttttaa tgccggactt
     1441 tcacgcaaat cgtcaatatc aggcttgaat gccaacccta aacaagcaat tttgatttga
     1501 tcgatagatt tacctgtttt ttgtaaggca taaattaccg cttcattcat ctttgagata
     1561 acccattcag gtttatgatc gtttacctca cgagcagtgc ggataatttt tgccaagtca
     1621 ggagttttat tcacaataaa ccaaggatct acagcaatgc agtgtccacc tacaccacaa
     1681 cccggttgta agatattgac tcgagggtgt ctgttagcca aagcaattag ttcccataca
     1741 ttaatatcta ggcgatcaca aataatagaa agctcatttg caaaagcgat attgacatca
     1801 cggaaactat tttcggttaa tttacacatt tctgcggtac gggcattggt aactatacag
     1861 tcgcctttta caaaagtttt gtataaattg accgcttgca cagagcattt atttgtcata
     1921 ccacccacaa tacggtcgtt ttcaatcaat tcatgcatca cttgtcctgg tagaactcgt
     1981 tctgggcaat gggcgatacg aatatcagaa tcttcacctg cttgctgagg gaagcttagc
     2041 tctggacggt actcggcaag ccactcagcc atctgttctg ttgcaccaac cggagaggtt
     2101 gattctaaaa tgactaaatt acctttttcc agcacaggtg caattgcttt gcaagcagct
     2161 tgaatataac ttaaatcagg ctcataatcc tcgcctttaa aaggcgttgg tacagcaatt
     2221 aaaaatgctt ctgctggttc aggtgtcagt gtggctttta attttccgct agatacacat
     2281 tggtgaacag caacatctaa atcaggctcg acaatgtgaa ttttcccttg attgatatgg
     2341 tctacggcgt gctgattaac atctacgcca attacattta gaccgtgttg ggcaaataca
     2401 gtggcggttg gtaagccaat atagcctaag cctactactg aaatggtatt aaagttcgac
     2461 atacatttat cccttattat tgttttgatt gaaatactga gagaatttgt ttcacagctg
     2521 tgccatcgcc ataagggttg tgagcctcag acatttttgt ataagcagct tgatcattga
     2581 gtaactcagt aacttgttta ataatttcag attgtgcagt tccaacaagt tttactgttc
     2641 cggcttctac agcttccggt ctttctgttg tgtctctcat tactaatacc ggttttccta
     2701 atgatggggc ttcttcttga atacctccgg agtcagttaa gataaggtaa gactctctca
     2761 ttaaataaac aaaggagaga tagtcgcaag gctcaatgag atgcacattg gatttatccg
     2821 ctaataggcg attgaccggt tctcttacat ttgggttgag gtgtacgggg tagataaatt
     2881 gaacgtcagg gaaatcagac gctaatgttg aaacggcttt acagatgttc tcaaaaccat
     2941 taccgaaatt ttcgcggcga tgtccggtga tcaaaatagt acgcttactt tttatataag
     3001 aaaatttctc ttgtagcgag cttaataaat caatattttc atctaatttt ttgcaagcga
     3061 ggaataaggc atcaattacc gtattacccg ttacataaat ggtgccctca gcaatatttt
     3121 cgttgagtag atttttcttg gtgctttgtg ttggtgcgaa atggtatttt gccaaagcag
     3181 ctgttaattt gcgattgcct tcttccggcc aaggagagta gatattatgc gttcttagac
     3241 ctgcctcaat atgagcgata tcaatttggt tgtaataggc tgctaatgaa gaggcaagag
     3301 tggttgctgt atcgccatga acaaaaatag tagttggttt gtattcggca ataacaggtt
     3361 gaattttttc taaaatagca gaagttacag tagaaagtgt ttgcttattg ctcataatat
     3421 ccaggtcata atctggagta atttcgaata attctaaaac ttggtccagc atttggcggt
     3481 gctgagcagt aacacagact ttaaaattta gaccgctttt cttaaagccc tcaactaagg
     3541 gagccatttt gattgcctca ggacgagtac cgaagacgat gagatagcgc atttattact
     3601 cctttaaaaa tatagataaa actaaaatac actagatgta agtaattttg ttaaattagt
     3661 tat
//
LOCUS       DANLZC010000185         3619 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.185, whole genome shotgun sequence.
ACCESSION   DANLZC010000185 DANLZC010000000
VERSION     DANLZC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.185"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            8..370
                     /locus_tag="PXE44_002383"
     CDS             8..370
                     /locus_tag="PXE44_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL5589842.1"
                     /translation="MKPTNKADFQRVINAAGYSMKGLKAAYINEAAFRQEIWCATILF
                     PLGLILGETNIEKALLVGTVLLVLVTELLNSAVESVVDRIGADFHELSGRAKDIGSAA
                     VFMAMVLLAITWILILFC"
     gene            407..2050
                     /locus_tag="PXE44_002384"
     CDS             407..2050
                     /locus_tag="PXE44_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDL5589843.1"
                     /translation="MRLKKTLLSVALLSATSAMAYETGKTYRFTVLHTNDIHGHFWQS
                     DKGEYGLAAQKTLVDNIRKEVAEKGGSVLMLNAGDANTGVPESDMQNARPDIEGMNAI
                     GYDAMVLGNHEFDYPLQILSMQEKWAKFPFISANVINKKTDKPIVKPYVMLERGGLKI
                     AVIGLTTEDTAKLGNPDVTENAFFKNPIETAKNTLAEINQKEKPDVRIALTHMGYYFD
                     GKHGTNAPGDVTMARTLEKGAFDLIIGGHTHDTVCIDEKGQFKAKYTPGEECKPDFQN
                     GTWIVQAGEWGKFVGRADFEFKDGKATLVKYELIPVNLKKTIKKEDGTKEYRLYQPEI
                     KADEATYTLLKKYQDEGDKKLNIEVGRVKGLLDGKREHIRFQQTNLGRLIAQSQMERV
                     KADIGIMNSGGIRTSLEEGVITYKHILTVQPFGNMIATVDLKGQELLDTLTTIALKEV
                     DTGAYPQFAGISMVVDRTAKTISDVKIGGKPFDKEKTYKVSLPDYVAAGGDGYPLLKK
                     HPSYVNTGFIDAEMLKKFFEENKIIEADKYDPKNQIIFK"
     gene            complement(2257..3126)
                     /locus_tag="PXE44_002385"
     CDS             complement(2257..3126)
                     /locus_tag="PXE44_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL5589844.1"
                     /translation="MSSRGKILNTISSLQNSLTKTEKKIAAAILSQPELLSQCSLSEV
                     AKQLDVGEATFIRFCRTLGFKGYTDFKLELAIELATQNQDNRVLLDTDVSESDTSKDI
                     AEKLKVSLDNVIEETINLLDFNVLEKVVEELRKAKRIFLFGVGSSGLTAEDAKHKLMR
                     IGLQTDAVTNNHFMYMQAALVKEGDLVIGISHSGYSEEIVKSLRFSRANKATTVAITH
                     NLRSPVTEEANYVLINGNRQGHMQGDSIGTKMSQLFVLDLIYTLLVKAEPENALKQKQ
                     KTLNVILEQRVKG"
     gene            complement(3136..3600)
                     /locus_tag="PXE44_002386"
     CDS             complement(3136..3600)
                     /locus_tag="PXE44_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL5589845.1"
                     /translation="MILADLSRDDYLKSLPPVFARLCKKLKSLDLVNLELGWQELENG
                     IRMNVMSFETTPAEGKKAELHRKFIDIQLLIDGEEMIEYGLSQPDLSKYDEYRDEDDY
                     QLTAEIDDKNNLILKPNMFAIFLPYEPHKPGNSVNGNKTLKKLVVKVPVELI"
BASE COUNT         1167 a          695 c          652 g         1105 t
ORIGIN      
        1 atttactatg aaaccaacca ataaagcaga ttttcaacgt gtgatcaatg cagctggcta
       61 ctctatgaag gggttaaaag ccgcttatat taatgaagcc gcctttcgcc aagaaatttg
      121 gtgcgctact attttattcc ctctagggct tattctaggc gaaaccaata ttgaaaaagc
      181 attattagta ggcacagttt tacttgtttt agttacagaa ctactcaata gtgcggttga
      241 atctgttgtt gaccgcattg gggctgattt ccacgagctt tctggtagag ctaaagatat
      301 tggctctgca gctgtattta tggcgatggt tttactcgct ataacttgga tattaatttt
      361 gttttgttaa ctctattttt caaccaacac tcttactgag gtatttatgc gcttaaaaaa
      421 aacattactt tcggtagcac tacttagtgc aacatcagca atggcctacg agacaggtaa
      481 aacatatcgt tttacggttt tacatactaa cgatatacac ggtcactttt ggcaaagtga
      541 taaaggcgaa tacggtctcg ctgctcaaaa aacattggtt gataatattc gcaaagaggt
      601 cgcagaaaaa ggtggttcgg tattaatgct gaatgcaggt gatgcgaata caggcgtgcc
      661 ggagtcagat atgcaaaatg cacgtcctga tattgaagga atgaatgcga ttggttacga
      721 tgcaatggtt ctaggtaatc acgaatttga ttacccactg caaattctct ctatgcaaga
      781 aaaatgggct aaattcccct ttatttcggc caatgtcatt aataaaaaaa cggataaacc
      841 cattgtaaaa ccttacgtga tgttagaacg aggcggacta aaaattgctg ttatcggatt
      901 aacaaccgaa gataccgcaa aattaggtaa tccagatgta acggaaaatg cctttttcaa
      961 aaaccctatt gaaacagcaa aaaacacatt agctgaaatt aatcaaaaag aaaaacccga
     1021 cgtacgtatc gctttaaccc atatgggtta ctattttgac ggtaaacatg gtactaatgc
     1081 accaggtgat gtaacaatgg cccgtacctt agaaaaagga gcgttcgatt taatcattgg
     1141 cggtcatacc cacgatactg tttgtattga tgaaaaaggt caattcaaag ccaaatatac
     1201 gccgggtgag gaatgtaaac cagacttcca aaacggtact tggattgtgc aagccggcga
     1261 atggggcaaa tttgttggac gggctgattt tgagtttaaa gatggaaaag ctaccttggt
     1321 aaaatatgaa ttaattccgg tcaacttaaa aaaaaccatt aaaaaagaag acggtactaa
     1381 agaataccga ctttaccagc ctgaaatcaa agccgatgaa gcaacctata ccttattgaa
     1441 aaaatatcaa gatgagggcg ataaaaagct taatattgaa gttggtcgtg tgaaagggtt
     1501 attagacggc aaacgtgagc atattcgctt ccaacaaacc aatctcggtc gtttaattgc
     1561 acaatcgcaa atggaaaggg taaaagccga tatcgggatt atgaactccg gcggtattcg
     1621 tacctccctt gaagaaggtg taataactta taaacatatt ctgactgttc aacctttcgg
     1681 taatatgatt gcaacggtgg atttaaaagg acaagaattg ttagataccc tcaccactat
     1741 tgctctgaaa gaagttgata ctggcgctta tcctcaattt gccggcattt caatggtagt
     1801 cgaccgtaca gcaaaaacta tttctgatgt taaaatcggt ggcaaacctt ttgataaaga
     1861 gaaaacttat aaagtttctc tgcctgacta tgttgcggct ggtggcgatg gctacccatt
     1921 actgaaaaaa caccctagct atgtcaatac aggctttatt gatgccgaaa tgctgaaaaa
     1981 attcttcgaa gaaaataaaa ttattgaggc agataaatac gatccaaaaa accagatcat
     2041 tttcaaatag agctatagaa aatgaaactc actctggcag tctctgctca aaagagctgt
     2101 ggctgcctgt agattgtttt cttactttca agtgttatag gaaatcgata aaaaatccct
     2161 accaaattaa ttcatcacca tatgatgaaa attaaaaaac ctctggcaat actttgccaa
     2221 aagtctttga cttttatagg gtaatatcac tgcttattag cctttaacac gttgttccaa
     2281 aatcacattc agtgtttttt gtttctgttt caacgcattt tcgggttcgg ctttaactag
     2341 taaggtataa attaaatcaa gaacgaaaag ttgtgacatt ttagtaccga tagaatctcc
     2401 ttgcatatgc ccctgtcgat taccattaat taacacataa ttagcctctt ccgtgacggg
     2461 ggaacgtaaa ttgtgtgtaa tcgcaaccgt tgtcgcttta tttgcacgag aaaaacgtag
     2521 tgatttcaca atttcttcag aatagcctga atgactaatc cctataacta aatccccctc
     2581 tttcaccaat gccgcctgca tatacataaa atgattattt gtgactgcat cggtttgtag
     2641 cccaatacgc attaatttat gttttgcatc ttctgcggtt aacccagaag aacccactcc
     2701 aaataaaaag atccgttttg ccttacgtaa ttcttcaaca actttttcca acacgttaaa
     2761 gtcaagcaaa ttaatagtct cttcaataac gttatctaaa ctcactttga gcttctcagc
     2821 aatatctttc gatgtatccg attctgaaac atccgtatca agcaatactc tattatcttg
     2881 attttgcgta gccaactcta ttgcaagctc taatttaaag tcggtataac ctttaaaacc
     2941 taatgtacga caaaaacgaa taaaagttgc ttcaccaaca tctaattgtt tggccacttc
     3001 agataatgaa cattggctaa gtaattccgg ctgagataaa atagcagcag ctattttctt
     3061 ctctgttttg gttaaactat tttgtagcga gctaattgta ttaagaattt ttccccgact
     3121 agacatagta gcctcctaaa tcaattcaac aggtactttc accaccagtt ttttcaatgt
     3181 tttgttgcca ttcaccgaat ttccaggctt atgtggttca taagggagga aaatagcgaa
     3241 catatttggc ttgaggatca agttattttt atcatcaatc tcagctgtta attggtagtc
     3301 atcttcatct cgatattcat catacttaga taaatctggc tggcttaacc cgtattcaat
     3361 catttcttcg ccgtctatca ataattgaat atcgataaat tttcggtgta actctgcttt
     3421 tttcccctca gctggcgtag tttcaaatga cattacattc atgcgaatac cattttctaa
     3481 ttcttgccag cctaattcca aattaactaa atctaaactt tttaattttt tacaaaggcg
     3541 agcgaatact ggtggaagag attttaaata atcatctctt gataaatcgg ctaatatcat
     3601 ataattctcc taaaaataa
//
LOCUS       DANLZC010000186         3609 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.186, whole genome shotgun sequence.
ACCESSION   DANLZC010000186 DANLZC010000000
VERSION     DANLZC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.186"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            8..2083
                     /locus_tag="PXE44_002387"
     CDS             8..2083
                     /locus_tag="PXE44_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL5589846.1"
                     /translation="MKIKKYSLSLITLAIVAPQVLAETTSQAELNAIEVKEKLAATTG
                     YIPVYTTVGTKTLTSLARTPFSINVVSDDLMHDQAVRSVTDSVAYTSGMMGGYRGNNS
                     LIEISIRGIGNKSDGSTIPTYLNGSRYQTAFEIDPFFLESINVIKGPNSILYGQANPG
                     GVMDIITKKAGSNPHRHLQFVVGNHQRYQIGLDIEENLSNTLSARLIGQLERTNWNTQ
                     FVKEKGGAFAPSILWKPTTNTELNVYAYYAKKPKAGDRNFLPKEGTINSVNGKKLPYD
                     MFLSDLNYHGLKAEQMQVGYSFSHKFTPNLTFRQNASYHNGEENFKNLVYWGDVGSVL
                     ERRARHWDVKTQEINLDNQLEWVFSTANINHTLLAGIDYKRVRKDLDNYMGIAPDFDW
                     QNPVYGVTVTEPSLFSSEVKHLKQLGVYLQDQIEIGNFDFLLGGRYDRAKNSNQDRLN
                     SKTENNQEGKFTWRTGVVYNFENGIAPYISYSTSFVPSAEKDAQGNFLKPTTAGQWEV
                     GMKYQPTQDTLLTLAGFKITQKDLANYQWQTRSYEQIGEVQTKGIEVSLNQQLSDKFS
                     IAASYAYLKKEITADKDGTTIGKTQWGVPRHQASLWAKYKFFDKLTAGVGVRYMGTTF
                     GDNQNTFTVPAYTLYDMMLAYDFMPNLNLQLNAQNLTNKKYVASCANNFSCFYGKDRN
                     LSATLNYHF"
     gene            2127..3563
                     /locus_tag="PXE44_002388"
     CDS             2127..3563
                     /locus_tag="PXE44_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3327 domain-containing protein"
                     /protein_id="HDL5589847.1"
                     /translation="MKKTTACILTFLVSSVLYANNITIELKQNDFVQGKLIQKAQQPL
                     SLSIIFPDQSSRLLAQNVFGEQDFMFKSELIGTATFSILANGATVSDQDFTLTIERHI
                     PISAQIAPPKVIENTQLEELSQKIAKNPEKQTALINDFWDKAKQAGTPLIEPLDEQQS
                     RVTFLWRGATKNARIWGGVSTEHDFMQRFGETDIWYKSYIVPNDTLVEYKLAPDVPTL
                     PVDENTQRRALLATAQADPLNKTAYFEKIGQNIHNTDKFNYASLLKLPNAPTQSFLTE
                     APNIAKGKLQQLIFESATLGNKHRLFVYLPDGFSAEKNYAVLYLFDGVEYTERVPTKT
                     ILDNMIAQKVILPTVAVFIENPSSESRRVELPANPIFTQVLAKELVPFVEKQLNIKTK
                     ERIIGGSSYGGLSSAYTVLNYPNVFSKALILSGSFWWQPKGTPSEQSHYIAQQFIQKE
                     KLPLCFFISAGFYESGKSAICKRSNYFA"
BASE COUNT         1199 a          725 c          680 g         1005 t
ORIGIN      
        1 taaactaatg aaaataaaaa aatattcact ttctctaatc actttagcga ttgtagctcc
       61 tcaagtatta gcagaaacaa cctctcaagc agaactaaat gctattgagg tcaaagagaa
      121 attggcagct accaccggct atattcctgt ttatacgact gtaggaacga aaacattaac
      181 ctctttggct cgtacacctt ttagtatcaa tgttgtctct gatgatttaa tgcatgacca
      241 agctgttcgt tctgttaccg attcagtggc ttatacctcc ggtatgatgg gtggctatcg
      301 tggtaacaat tcgcttatcg aaatctccat tcgaggcatt ggcaataaaa gtgatggcag
      361 tacaattccg acctatttaa acggttctcg ctaccaaact gcatttgaaa tcgatccgtt
      421 cttcttagaa agtatcaatg tgattaaagg tccaaactcc attttatacg ggcaagctaa
      481 tcccggtggc gtaatggata ttatcaccaa aaaagccggc agtaacccac atcgccattt
      541 gcaatttgtg gtgggaaacc atcaacgata ccaaatcggg ctggatattg aagaaaatct
      601 ctccaatacc ttatccgctc gtttaatcgg tcaattagaa cgaacaaact ggaataccca
      661 atttgtaaaa gaaaaaggcg gtgcttttgc tccaagcatt ttatggaaac cgactacaaa
      721 tacagaactt aatgtttacg cttactatgc aaaaaaaccg aaagccggtg atcgtaactt
      781 tttaccgaaa gaaggaacga ttaatagcgt aaacgggaag aaactaccct atgatatgtt
      841 cctcagcgat ctgaactatc acggtttaaa agccgaacaa atgcaagtag gttacagttt
      901 ctcacataaa tttacgccaa acctaacgtt ccgccaaaat gcaagttacc ataatggcga
      961 agagaatttc aaaaatctag tttattgggg cgatgtcggt tcggttttag aacgtagagc
     1021 tcgccattgg gatgtcaaaa cccaagaaat caacttagat aaccaattag aatgggtatt
     1081 tagcacagca aatataaatc ataccttgtt agccggtatt gattataaac gagtgcgtaa
     1141 agatcttgat aactatatgg ggattgcccc tgattttgat tggcagaacc ctgtttatgg
     1201 cgtgaccgtt actgagccta gcttattttc aagtgaagtg aagcatttaa aacaacttgg
     1261 ggtttatcta caagatcaaa tagaaatcgg taattttgac ttcctgttag gcggtcgtta
     1321 cgatcgagca aaaaacagca accaagatcg tttgaacagt aaaacagaga ataaccaaga
     1381 aggaaaattt acttggcgta caggcgtagt ttacaacttt gaaaacggta tcgctccgta
     1441 tatcagctac agcacttctt ttgtgccatc ggctgaaaaa gatgctcaag gaaatttctt
     1501 aaaaccaaca actgctgggc aatgggaagt gggaatgaaa taccaaccta cccaagacac
     1561 cttactgacc ttagcaggat ttaaaattac acaaaaagat ttagccaatt atcaatggca
     1621 aacccgctct tatgagcaaa ttggtgaagt gcaaaccaaa ggaattgaag tgagtttaaa
     1681 tcagcaatta tccgataaat tttcaatagc ggcttcttat gcttacttaa aaaaagagat
     1741 tactgcagat aaagatggca cgacgattgg aaaaacgcag tggggagtgc ctcgccatca
     1801 agcatctctt tgggcaaaat ataaattctt tgataagctg accgccggtg taggtgtacg
     1861 ctatatggga acgacttttg gcgataacca aaatactttt accgtaccgg catacacact
     1921 ttacgatatg atgctagctt atgattttat gcctaactta aacttacagc tcaacgcaca
     1981 aaacctgacg aataaaaaat atgtcgcaag ctgtgccaat aatttttcct gtttctatgg
     2041 aaaagatcgc aatttaagtg caacgttaaa ctatcatttt taatctcaac agccctcaaa
     2101 tgagggctta aacttttgca aaaaatatga aaaaaacgac cgcttgtatt cttacttttt
     2161 tggtttcatc tgtgctttat gctaacaata taaccattga gcttaaacag aatgattttg
     2221 tgcaaggcaa attgattcag aaggctcaac agccactttc cttgtcgatt atttttcctg
     2281 accaaagctc tcgcctgctg gctcaaaatg tctttggtga acaagatttt atgtttaaat
     2341 cagaactaat cggcacagct actttttcta ttctagcgaa tggtgcaacc gtttcagacc
     2401 aagattttac cttaacgatt gagcggcata ttccaatctc ggcacaaatt gctccaccta
     2461 aagtaattga aaatacacag cttgaagaac tcagccaaaa aattgcaaaa aatccagaaa
     2521 aacagaccgc tttaattaac gatttttggg ataaagcaaa acaagccggc acaccattga
     2581 ttgaaccatt ggacgaacag caaagccgtg taactttttt atggcgtgga gcaacgaaaa
     2641 atgcccgtat ttggggcggt gtgagtactg aacacgattt tatgcaacgc tttggtgaaa
     2701 cggatatatg gtataaaagt tatatagtgc ccaatgacac tttagtggaa tacaaattag
     2761 ctccagatgt gccgacattg cccgttgatg agaataccca acgcagagcg ttactggcta
     2821 cggctcaagc cgatccactc aataaaacag catacttcga gaaaatcgga caaaatattc
     2881 ataataccga taaattcaac tacgcttcgc tacttaaatt gccaaacgca cctactcaat
     2941 cttttttgac tgaagctcca aatattgcaa aaggtaaact gcagcagttg atttttgaga
     3001 gtgcaacact cggcaataaa cacagattat tcgtctatct gcctgacggt tttagtgcag
     3061 aaaaaaacta tgcggttctc tatttatttg atggtgttga atataccgaa cgagtgccaa
     3121 ctaaaaccat tttagataat atgatcgctc agaaagtgat tctgcctact gtagcggtgt
     3181 ttatcgaaaa tccatcgagt gaatcaagac gtgtcgagct gcctgccaat cccatattta
     3241 cgcaagtatt ggcaaaagaa cttgtacctt ttgttgaaaa acagctcaat ataaaaacaa
     3301 aggagcgaat tattggcggc tctagctatg gcgggctatc ctctgcttac accgtactca
     3361 actatcctaa tgtatttagt aaagcattga ttctatccgg ttcattttgg tggcaaccca
     3421 aaggcacacc tagtgaacaa agccactata ttgctcaaca atttatccaa aaagaaaaat
     3481 taccactctg ttttttcatc agtgcaggtt tttatgaaag cgggaaatcg gctatttgta
     3541 agcggtcgaa ttattttgca tagaattaca ctcaattttg catagataaa aattaaaaac
     3601 tatgcaaaa
//
LOCUS       DANLZC010000187         3545 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.187, whole genome shotgun sequence.
ACCESSION   DANLZC010000187 DANLZC010000000
VERSION     DANLZC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.187"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            46..1233
                     /locus_tag="PXE44_002389"
     CDS             46..1233
                     /locus_tag="PXE44_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589848.1"
                     /translation="MSALINLFYLYDPWFFHFLRMSFVVGLVACGYLVFQIIKGKSNG
                     IIIPKDSLIAIIALIGFSIIPLLINSTKEIGVLAMYVKMLILFVFGIAIYNVFYHNQQ
                     GKTLFVRDLKIGIAIQWICGVTALLGLPFMVDFLLSSNAMMPRFFGSEQEYRLYNITS
                     SAFFQLSIFYLMLLHFLLAYNKKTDVVNEWFLFLLLCVGLISGRTFLLMSVISIALYF
                     KWKYILPLIAFTALILFLAIVFPENRYVEHALEPVINLLSGTGKVSSSTDNLVKNHLF
                     LPTLNQFISGDGLYYTETGGYYGGSDSGYIRQVLYGGVSYMLACFAFTYYFVRKVAVN
                     WFEGSWIFTLSTIGILAILNIKADTFAFPGLMMLFLMFLSLFGTSGRNVTFFCKNKEA
                     EYV"
     gene            1226..2191
                     /locus_tag="PXE44_002390"
     CDS             1226..2191
                     /locus_tag="PXE44_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL5589849.1"
                     /translation="MFSIIVPSYNRNQEINALLESLKQQTAYNFEVIIVDDCSKIPVS
                     ETLGILNEFNDISTKEKVRELLTRDSLSHIYSYPFDVKVVRNNPNVGAAQSRNVGATN
                     ATQEWLLFLDDDDRFMPEKCERLAAEIANNPTANFVYHPAKCEMVNEGFTYVTKPFKH
                     KSELTKENILKANKIGGMPMIGLKKSLFCKIGGLSDRLRSLEDYDFLLKLLEEPSFEP
                     LYVDEALTYCTFHTKRSSVSTDTTNTEKAIEYIQQTYIKTPEANNFKLNSLYILSYPH
                     VMNLSRKAAGYYFEMFKQTKNIKQLIIALVIFISPKLAINLKRFM"
     gene            2191..2991
                     /locus_tag="PXE44_002391"
     CDS             2191..2991
                     /locus_tag="PXE44_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5589850.1"
                     /translation="MKFSVLMSLYIKEQPQYLRECFESLKVQTVQADEIVVCFDGAVT
                     PELEAIVEEYSAILPINAVKFPQNRGLGKTLNDGLTYCKNEWVFRMDTDDICMPERFE
                     KQVAFIEQNPETVIFGGQIAEFGQDIHDLVAYRNVPTTVEEIVRFTRLRCPFNHMTVA
                     YQKSKVLEVGGYQDLQEDYYLWIKLIAQFKQVANLPDLLVYARVGNGMVGRRRGMAQA
                     EAEWRLYKLKNRCQIHDPISGFGIFIMRAIPRLLPVSALKKVYGLLRK"
     gene            complement(3028..3534)
                     /gene="msrA"
                     /locus_tag="PXE44_002392"
     CDS             complement(3028..3534)
                     /gene="msrA"
                     /locus_tag="PXE44_002392"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL5589851.1"
                     /translation="MQNIREIYLAGGCFWGVESFMQRIKGVIKAQSGYANGNTDNPSY
                     QEVCAGSGHAEVVKVTYDANQISLAKLLDYFFKVIDPVSINKQGGDIGIQYRTGIYYT
                     DPQDEPVVTAALAQLQTQFDQPLAVENQMLEQYFPAEEYHQEYLVKNPNGYCHIDIQL
                     MNEILKNQ"
BASE COUNT         1035 a          601 c          718 g         1191 t
ORIGIN      
        1 caattaaacg taattttact cagcgaaatt cataataggt ctgaaatgtc agcactcatt
       61 aacttatttt atctttacga tccgtggttt ttccattttt taagaatgtc gtttgtagtc
      121 gggcttgttg cttgtggcta tttggttttc caaataatca agggcaaatc gaatggcatt
      181 attattccta aagatagcct gattgcaatt attgcattaa tcggctttag cattattcca
      241 ttattgatta acagtacaaa agaaattggt gtgctggcaa tgtatgtcaa aatgctgatt
      301 ttgtttgtgt tcgggattgc gatttataat gttttttatc ataaccaaca aggtaaaact
      361 ttattcgttc gtgatttaaa aatcggcatt gctattcaat ggatttgtgg tgtgacagcc
      421 ttattaggtt tgccgtttat ggtggatttt ttattatcca gcaatgcaat gatgccacgt
      481 ttttttggtt cggaacaaga ataccgttta tataacatca cttcttccgc ctttttccaa
      541 ttaagcattt tttatttaat gctattacat tttttattgg catataataa aaaaacagat
      601 gtggtgaatg agtggttttt atttttatta ttatgtgttg gtttaatttc cggtagaacc
      661 tttttattaa tgtcagtaat aagtattgca ctctatttta aatggaaata tatattgcca
      721 ctgattgcct tcactgcgtt aattctattt ttggcgattg tttttccgga aaaccgttat
      781 gttgaacacg ccttagaacc ggtaattaat ttattgtccg gcaccggaaa agtcagctct
      841 tcaaccgata atttagtgaa aaaccatctg tttttaccga ctttaaatca gtttatttcc
      901 ggtgatggtt tatattacac cgaaactggt ggctattatg gtggatcgga ttccggctat
      961 attcgccaag tattatatgg tggagtaagt tatatgttgg cgtgttttgc cttcacttac
     1021 tattttgtac gcaaagtggc ggtgaattgg tttgaaggca gttggatttt taccttatcc
     1081 accatcggca ttttagctat tttaaatatt aaagcagata cctttgcttt cccggggctg
     1141 atgatgctgt ttctaatgtt cttgtcgctg tttggaacaa gcggtcgaaa tgtaacattt
     1201 ttttgcaaaa acaaagaggc tgaatatgtt tagtattatt gtgccttctt ataaccgtaa
     1261 tcaagaaatc aatgcgttgc ttgagagttt aaagcaacaa actgcatata attttgaagt
     1321 gatcattgtt gatgactgct caaaaatacc ggtcagtgaa actttaggaa ttttaaatga
     1381 atttaacgat atttctacta aagaaaaagt aagagaatta cttactagag atagtttatc
     1441 tcatatttat agctatccat ttgatgtgaa agtggtgcga aataacccaa atgttggggc
     1501 ggcacaaagc cgaaatgtag gggcaactaa tgccacacaa gaatggctac tgtttttaga
     1561 tgatgatgac cgctttatgc ctgaaaaatg cgaacgttta gcggcagaga ttgcgaacaa
     1621 tccaactgcc aattttgtgt accacccggc aaaatgtgaa atggtaaatg agggttttac
     1681 ttatgtcacc aagccgttta aacataaaag tgagctaact aaagaaaaca ttctaaaagc
     1741 gaataaaatt ggcggaatgc cgatgattgg cttgaaaaaa tcactctttt gtaaaattgg
     1801 tggattatct gaccgcttgc gatcacttga agattatgat ttcttactga aattattgga
     1861 agaaccgagt tttgagccat tgtatgtgga cgaagccttg acctattgta ctttccatac
     1921 caaacgctcc agtgtttcaa ccgataccac taatacagaa aaggctattg agtatattca
     1981 acaaacgtat atcaaaacac ctgaggcgaa taattttaag ctcaattcgc tttatatttt
     2041 aagttatcca cacgtaatga atttatcccg caaggcagcc ggttattatt ttgagatgtt
     2101 taaacagact aaaaatatca aacaactgat tattgcgttg gttattttta tttcgccgaa
     2161 gttggcgatt aatttgaaaa ggtttatgta atgaaatttt ccgttttaat gtcgttatac
     2221 attaaagagc aaccgcaata tttaagagaa tgttttgaaa gcttgaaagt acaaactgtg
     2281 caggctgatg aaatcgttgt ttgttttgat ggtgcagtta cgcctgaatt agaggcgatt
     2341 gtagaagaat atagtgcaat tttgccgatt aatgcggtga aattcccaca aaatcgaggt
     2401 ttaggtaaaa cgctaaatga tgggctaacc tattgtaaga atgaatgggt tttcagaatg
     2461 gatacagacg atatttgtat gccggaacgt ttcgagaaac aagtggcatt tattgaacag
     2521 aatcctgaaa ccgtgatttt tggcggccaa attgctgaat ttgggcaaga tattcacgat
     2581 cttgtcgctt accgcaatgt accaaccacg gtggaggaaa tcgtcagatt cactcgcttg
     2641 cgttgcccgt ttaaccatat gacggttgct tatcagaaat ccaaagtgtt ggaagtcggt
     2701 ggctatcaag atctgcaaga agattactat ttatggatca aactcattgc tcaatttaaa
     2761 caagtggcaa acttgccgga tttattagtg tatgcccgag tcggtaacgg aatggttggc
     2821 agacgccgtg gaatggccca agcagaggca gaatggcgtt tatataaatt aaaaaaccgc
     2881 tgccagattc acgatccgat ttcaggtttt gggattttca taatgcgtgc aattcctcgt
     2941 ttattaccgg tttcagcact gaaaaaagtg tacggtttgc tcagaaaata aagctaaaaa
     3001 aaccacagat tgctctgtgg ttttttgtta ttgattcttc aaaatctcat tcattaattg
     3061 aatatcaata tggcagtagc cgttcgggtt ttttactaaa tattcttggt ggtactcttc
     3121 ggcagggaaa tattgctcta acatttggtt ttccactgcc agcggctgat caaattgggt
     3181 ttgtaattgt gcaagtgcgg cggttaccac cggttcgtct tgtgggtcgg tataataaat
     3241 accggtgcgg tattgaatac cgatgtcgcc accttgtttg ttaatgctca ccgggtctat
     3301 tactttaaag aagtaatcta gtaattttgc caagctaatt tgattggcat cataagtcac
     3361 ttttaccact tcagcgtgtc cgctaccggc acacacctct tgatagcttg ggttatcggt
     3421 attaccgttg gcatagcctg attgtgcctt aatgacacct ttaatccgtt gcataaaaga
     3481 ttccacgccc cagaaacagc caccggctaa gtaaatttca cgaatatttt gcatattacc
     3541 tctct
//
LOCUS       DANLZC010000188         3405 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.188, whole genome shotgun sequence.
ACCESSION   DANLZC010000188 DANLZC010000000
VERSION     DANLZC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.188"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            23..2473
                     /locus_tag="PXE44_002393"
     CDS             23..2473
                     /locus_tag="PXE44_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HDL5589852.1"
                     /translation="MTVEVGGVVITADLEFENLLRDTQKVDKAMDDIGKSTLKAEKGL
                     KGLETQATKTAIAVNQATNKMKNIRGVAGQLGYQIQDISIMLQMGSNASTVIAQQGSQ
                     ILSIFGPMGAVAGAILAISGAIGGALLPSLFDSTDATELLAAAQKNLSEVVTQTESGV
                     SALSAKIIKLAKVSEDAAKAKIAVAMSDAKIAIKAAGDSSQKAFEQFDGFFGSFTNSS
                     QSIRNAIDELKRYESKGKDVGAVIKELGGTYGGAIAEVNALDSVTSEMSKTLGITQSE
                     ALGLIKLLAQLEQDKSPDNIKLLAIALSDLSDKNGWANEELNKLTRTINDNSVSALDA
                     ETSVRLLESAIKDLQNAIQNSQGALDGNVAKMNQLADAAERKAATIGKTERETAKYAA
                     TLLATTDEAKKNLDSTLSRIDASYDQIEAYQAEQKALQDKKTADAKAAREAEAAAKKS
                     AQAKKQVVEQLRQMATQYEIAVLRQKGMHLEAVKLEAGMRLGAAATKEQREEAEKLAQ
                     SIYGATAASQNFNAIQAQASPVTALDQRHQQQLAQIEEYKILYPQKIMEAEAARAAIE
                     EQYRQQRMEAQWEEWKQASDGARMFGDAVDAVGSSATSTITGLLNGTMSVRDAFASIA
                     NTILNSVVQSLVEMGMAQVKQMIMGQTAAKASMAAQMVQAKALAASFAPAASMVSLAT
                     QGANAIPAQAAITSTMSMAQVAAVTGRKLGGPVSARQMYRVGENNQPEIFKASNGMQY
                     MIPGQSGRVFSNKQSQGQGGGESKTVAVIVNQTNHFSSDEASANQAAVAKALSNQIKA
                     SVRLELQSQMRAGGVLAR"
     gene            2477..2806
                     /locus_tag="PXE44_002394"
     CDS             2477..2806
                     /locus_tag="PXE44_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5589853.1"
                     /translation="MQTFNFKVEQDYTVQNEPSVVILKFADGYEQTAPKGLNHNLKKY
                     QGITVKANKAEAVKIQQFLNAHGGYKKFNWRDRTSDETVTVRCRSWSSTKLGAVIEFT
                     LNFDEVI"
     gene            2806..3276
                     /locus_tag="PXE44_002395"
     CDS             2806..3276
                     /locus_tag="PXE44_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5589854.1"
                     /translation="MPKAPPKKMASELIKLEQDALIELWEIDLTHIYSSSNPNQRGEI
                     FRFHNGVSQTQQNIWWQGNEYQAYPIAADGFEISGQGPSNRPTLTVSNLYGVVTVLVE
                     DFGQPVEAKVSSEETVDGNIQMTVELLKKMDQIADQRYRRNQLNDLRTGGTLSK"
     gene            3284..>3405
                     /locus_tag="PXE44_002396"
     CDS             3284..>3405
                     /locus_tag="PXE44_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5589855.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRKLQDKNGNAG"
BASE COUNT         1158 a          671 c          804 g          772 t
ORIGIN      
        1 aaataatttt aaggaattgt ttatgacggt cgaagttggc ggagttgtaa ttaccgcaga
       61 tctagaattt gaaaatctgc taagagatac tcaaaaagtt gataaagcga tggatgacat
      121 cggaaaatca acattaaaag cagaaaaagg gctgaaaggg ctagaaacgc aggcaaccaa
      181 aacggcgata gcagtaaatc aagccacaaa caaaatgaaa aatatccgtg gagtagcagg
      241 tcaacttggt tatcaaattc aagacatctc aataatgctt caaatgggtt ctaacgcttc
      301 aaccgttatt gctcagcaag ggtcgcaaat cttaagtatt ttcggtccaa tgggagctgt
      361 tgcaggggcg atcttagcta ttagtggtgc tattggtggg gcgttactcc ctagtttgtt
      421 tgattcaact gatgcaactg agcttttggc agcagctcaa aaaaatctca gcgaagtagt
      481 tactcaaacg gaaagtggtg tttccgccct ttctgcgaaa atcattaaat tagcaaaagt
      541 aagcgaagat gcagcaaaag caaaaattgc agtagctatg agtgatgcaa aaatagctat
      601 taaggcagca ggggattcat cacaaaaggc atttgagcaa tttgacggct tttttggatc
      661 gtttacaaat tcaagtcaaa gtattcggaa cgcaattgac gaattaaaac gctatgaatc
      721 aaaaggtaaa gatgttggtg ccgtaataaa agaacttgga ggcacatatg gtggtgcaat
      781 tgccgaagtt aatgcgctgg attctgtgac aagcgaaatg tctaaaacgc ttggtattac
      841 tcaatctgag gctcttggtt tgataaaact gttggctcaa ttagagcaag acaaaagccc
      901 tgataatatt aaattactcg cgatagcttt atctgactta agcgataaaa atgggtgggc
      961 gaacgaagag ctaaataagc taaccagaac gataaacgat aattcagtat ctgctttaga
     1021 tgccgaaaca tcagtaagac tcttagagtc tgctataaaa gacttgcaaa atgcaatcca
     1081 aaactcccaa ggtgcattgg atggcaatgt agcaaaaatg aatcaacttg cagatgcggc
     1141 agaaagaaaa gctgcaacga taggcaagac tgagcgtgaa actgctaaat atgcagctac
     1201 gctattagct acaaccgatg aagctaaaaa aaatcttgat tccacattaa gtaggattga
     1261 cgcatcatac gatcaaatcg aagcctatca agccgaacaa aaagccttac aagataaaaa
     1321 gaccgcagat gcaaaagctg ccagagaagc tgaagcggct gcaaaaaaat ccgctcaagc
     1381 caagaagcaa gttgtcgaac agttgcgaca aatggctaca cagtacgaga ttgctgtttt
     1441 gaggcaaaag ggaatgcatc tcgaagccgt aaaattagaa gctggtatgc gtttaggagc
     1501 tgcggcaaca aaagagcaac gagaagaagc tgaaaaacta gcacaaagta tttatggagc
     1561 aaccgcagca agtcagaact ttaacgctat acaagcccaa gcatctcctg ttactgcatt
     1621 agaccaacga catcaacaac agctagctca gatcgaagaa tataaaatct tgtatccaca
     1681 aaaaataatg gaagcagaag ctgcgagagc tgcaatcgaa gaacaatacc gccagcaacg
     1741 aatggaagct caatgggagg agtggaagca agcgagcgac ggggcgagaa tgtttggtga
     1801 tgcggttgat gctgttggtt caagtgcaac aagcacaatt acggggctac taaatggcac
     1861 aatgtccgtg cgtgatgctt tcgcatcaat tgcaaatacg atcttaaata gtgtagtaca
     1921 aagccttgtc gaaatgggta tggctcaagt taagcaaatg attatggggc aaactgctgc
     1981 caaggcttct atggcggcgc aaatggtgca agctaaagcg ttagctgctt catttgcacc
     2041 cgcggcctct atggtttctc ttgccacaca gggggcgaat gctattcccg ctcaagctgc
     2101 gatcacgtca actatgagta tggctcaagt cgccgcggtg acaggtcgta aacttggcgg
     2161 ccctgtatca gctagacaga tgtatcgtgt cggcgagaac aatcagcccg aaatcttcaa
     2221 agcgagcaat gggatgcaat atatgatccc cggtcaaagc ggtcgtgtat tcagcaacaa
     2281 gcagtcgcaa ggtcaaggtg gaggcgaaag caaaacagta gctgtcatcg taaatcaaac
     2341 caatcacttt agcagcgatg aagccagtgc aaatcaagcg gctgttgcaa aagcattgag
     2401 caatcaaatc aaggcttccg tgagattgga attgcagtca caaatgcgag ctggtggagt
     2461 tctagcgagg taatcaatgc aaacatttaa tttcaaggtt gagcaagact acactgttca
     2521 aaatgagccc agtgtcgtca tactaaaatt tgccgatggt tacgagcaaa ccgctcccaa
     2581 agggttaaac cacaatctga aaaaatacca aggcattacc gtaaaagcga acaaagcaga
     2641 agcggtgaag attcagcaat ttctcaatgc tcacggtggg tataagaaat tcaactggcg
     2701 cgatagaaca agcgatgaga cggtaacggt tcgttgcaga agctggagct ccactaaatt
     2761 aggtgcagtc attgagttta cacttaattt cgatgaggtg atttaatgcc aaaagccccc
     2821 cccaaaaaaa tggcatcaga gttgataaaa ctcgaacaag atgccctgat tgagctgtgg
     2881 gagatagatc tcactcacat ctactctagc tcaaacccaa atcagcgcgg agagatattc
     2941 cgctttcaca atggcgtgag ccaaacacaa cagaacatct ggtggcaagg caatgaatac
     3001 caagcatacc cgattgccgc cgatggtttt gagatttcgg gtcaaggacc gtcaaatcgt
     3061 ccaacactca ctgtctctaa cctgtacggt gttgtcacgg tattagtaga agatttcggg
     3121 caacccgtag aggcgaaagt cagcagtgaa gagactgtag atggcaatat ccaaatgacg
     3181 gtggaactgc tgaaaaaaat ggatcaaatc gccgatcaac gctaccgtag aaatcagtta
     3241 aatgatttaa gaacaggtgg aacattatct aaataggtga agtatgccaa ttagtatttc
     3301 taaccaaatg aaactagatc ttgccaaact tgagcaaaat gcaatgctgg acttgtacga
     3361 ggttgatttg cgtaaattgc aagacaaaaa cggcaatgct ggtag
//
LOCUS       DANLZC010000189         3335 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.189, whole genome shotgun sequence.
ACCESSION   DANLZC010000189 DANLZC010000000
VERSION     DANLZC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.189"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(91..261)
                     /gene="rpmG"
                     /locus_tag="PXE44_002397"
     CDS             complement(91..261)
                     /gene="rpmG"
                     /locus_tag="PXE44_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL5589856.1"
                     /translation="MAAKGAREKIRLVSTAETGHFYTTDKNKRNMPEKMEIKKFDPVV
                     RKHVIYKEAKIK"
     gene            complement(273..509)
                     /gene="rpmB"
                     /locus_tag="PXE44_002398"
     CDS             complement(273..509)
                     /gene="rpmB"
                     /locus_tag="PXE44_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL5589857.1"
                     /translation="MSRVCQVTGKRPAVGNNRSHAMNATKRRFLPNLHTHRFWVESEN
                     RFVTLRLTAKGMRIIDKKGIDAVLAEIRARGEKI"
     gene            complement(741..1478)
                     /gene="radC"
                     /locus_tag="PXE44_002399"
     CDS             complement(741..1478)
                     /gene="radC"
                     /locus_tag="PXE44_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL5589858.1"
                     /translation="MEEKMNDFFDEDSQEPTMMPREKLLTQGAASLTDSELLAIFLRT
                     GTKAVPVLALAESVLMAFGSLRQLLNANIDDFCKIYGLGKTKYIQLQASKEMTKRYLA
                     QQMEFSEMIQAPYMAIMYFQTELEEEEREVFMVLFLDNQNRLIYKEKMFFGTINQTAV
                     HPREIIKRALKYNAAAIIVAHNHPSGSCLPSESDRSLTKKIEMACELVDIRFVDHIIV
                     GKGDYFSFEEEKLEMKEHSFLQISDRK"
     gene            1628..2830
                     /gene="coaBC"
                     /locus_tag="PXE44_002400"
     CDS             1628..2830
                     /gene="coaBC"
                     /locus_tag="PXE44_002400"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDL5589859.1"
                     /translation="MLQNKKILVGISGGIAAYKTIEFIRHLKKANAEVRVVLTPAAEA
                     FVTPLTLQAISGNAVSTSLLDPQAELAMGHIELAKWADLIVIAPASADFIARLRMGMG
                     NDLLSTLCLATASPILLAPAMNQQMYKQIAVQENLSVLVNRGVQLIGPNSGFQACGDV
                     GAGRMSEPEEIYQAVLAHFSNNQDLEGLNVTITAGPTREAIDPVRYISNHSSGKMGYA
                     IANAFAERGAKVTLISGPVNLPTPKNVERINVESAVEMEQQAVKFAQKSAIFIGCAAV
                     ADYRMAAVSDQKIKKTDANDELTLKLVKNPDIIANVANLAENRPFVVGFAAETQNVAE
                     YAKSKLERKNLDMICANDVSGGQVFGQDQNALHLFWKNGEKTLPLADKSKLAESLVQE
                     IVKHYREK"
     gene            2830..3147
                     /locus_tag="PXE44_002401"
     CDS             2830..3147
                     /locus_tag="PXE44_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589860.1"
                     /translation="MKLLVSHFHQGEGKFPTFSAGTAVRLGSECVNYPNWFACEIDGW
                     KTFVPSHFISEDKLICDYNPTELVAAQGERVEFLELHYQWAIVKRENEIGWLPCEILR
                     VIG"
     gene            3144..3326
                     /locus_tag="PXE44_002402"
     CDS             3144..3326
                     /locus_tag="PXE44_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL5589861.1"
                     /translation="MKIMQLTSNQYINDIKHILLSARQKAYQAVNSAMVEAYWKIGER
                     IVQEEKNMVKDQNEKV"
BASE COUNT          993 a          685 c          689 g          968 t
ORIGIN      
        1 attagtttaa tcatacaagc ggtctttttt cactaatttt ttgtatatga aaaacccagc
       61 attcgctggg ttttaaatct taaaacaaaa ttatttaatt tttgcttctt tgtagataac
      121 gtgtttacgc acaactggat caaatttttt gatttccatt ttttcaggca tattacgttt
      181 gtttttatct gttgtgtaga agtgaccagt ttctgctgta gaaactaaac ggattttctc
      241 acgagcgcct ttagctgcca ttttttagct ccttagattt tttcgccacg agcacggatt
      301 tcagctaata ctgcatcaat gcctttttta tcaataatac gcataccttt cgcagttaaa
      361 cgtaaggtta cgaaacggtt ttcgctctca acccagaaac ggtgagtgtg aaggtttggt
      421 aagaaacgac gtttagtcgc attcattgcg tgtgagcggt tgttaccaac tgctggacgc
      481 ttgccggtta cttggcaaac tcttgacatt tgaatttctc caataatttt aagcttaaaa
      541 atggttcggg cgatctcaca aaaggcgttg ctcaaccaac ccctccgatt ttgattgcct
      601 cgtcaggcat taacccgacc acagtctatg taataaagac tgccgattat accgatttca
      661 tttgttatat tcaagtagaa tttatatgcg tatcttttac tgtttcagga gaaatgttaa
      721 aggttacata aagcaagcgg tcattttcta tcagaaattt gcaaaaatga atgctccttc
      781 atctctaatt tctcttcctc gaacgaaaaa tagtcccctt tgccgacaat aatatgatcc
      841 acaaagcgaa tatcaaccaa ttcgcacgcc atttcgatct ttttagttaa acttctgtcc
      901 gactcactcg gcagacaaga acctgagggg tgattatgag ccacaataat cgccgcggca
      961 ttatatttta acgctctttt aataatttct cgggggtgaa cagcagtttg attaatggtg
     1021 ccgaagaaca ttttttcttt ataaatcaat cggttttggt tgtccaaaaa cagcaccata
     1081 aaaacttccc gttcttcttc ctccaactcg gtttggaaat acataatcgc catataaggg
     1141 gcttgaatca tctcgctaaa ttccatttgt tgagccagat agcgtttggt catctcttta
     1201 gaggcttgta actgaatgta tttggttttg cctaaaccgt aaattttgca aaaatcatca
     1261 atatttgcat tcagtaattg gcgtaacgag ccaaatgcca tcaacacact ctctgccaat
     1321 gccagcacag gcacagcttt cgtaccagtc cgtaaaaata tcgctaataa ttcggaatcg
     1381 gttaatgagg ctgccccctg tgttaataat ttctcacgcg gcatcattgt tggctcttga
     1441 gaatcttcat caaaaaaatc gttcattttt tcctccatca atttagttaa gcataaaaga
     1501 gccaaccttt gcagacaaag ggatttattc aatttttcga gcaagatcgc aaaaatagtg
     1561 aagttgtgag caactcctta ttccgctaaa atatgcaaaa taaatccacg ctttaaggat
     1621 agtaaagatg ttacaaaaca aaaaaatttt agtcggcatt tccggtggga ttgcagccta
     1681 caaaaccatt gaatttatcc gccacttaaa aaaggcaaat gcggaagtgc gtgtagtgct
     1741 aaccccagca gcagaagcct ttgtaacgcc tttgacctta caagcgattt ccggcaatgc
     1801 ggtttccact tcactactcg acccacaagc cgaactggcg atggggcata ttgagctggc
     1861 aaaatgggcg gatttaatcg ttatcgcccc ggcaagtgcc gattttattg ctcgcctgcg
     1921 tatgggaatg ggcaacgatt tactttccac tctctgcctt gcgactgcaa gcccgatttt
     1981 gcttgcaccg gcaatgaatc aacaaatgta caaacagatt gcggtgcaag aaaatctgtc
     2041 tgttttagtc aatcgtggcg ttcagctcat cggcccgaat agtggcttcc aagcctgtgg
     2101 tgatgtgggg gctggcagaa tgtctgagcc tgaagaaatt taccaagcgg tgttggctca
     2161 tttctccaac aatcaagatt tagagggctt aaatgtaacc attaccgccg gaccgactcg
     2221 tgaggcgatt gaccctgtgc gttatatcag caaccacagc tccggcaaaa tgggctacgc
     2281 gattgctaat gcctttgccg aacgtggagc aaaggttacg ttgattagcg gtccggtgaa
     2341 tttaccaacg ccgaaaaatg tggaacgaat taatgtggaa tctgcggttg agatggagca
     2401 acaagcggtc aaatttgccc aaaaatctgc gatttttatt ggctgtgcag cggttgccga
     2461 ttaccgaatg gcggcagtct ctgaccaaaa aattaagaaa accgatgcca atgatgaact
     2521 cacgctaaaa ttggtaaaaa accccgatat tatcgctaac gtcgccaatc ttgccgaaaa
     2581 ccgccctttt gtggttggtt ttgccgcaga aacgcaaaat gtggcggagt acgctaaatc
     2641 gaaactcgaa cgcaaaaatc tggatatgat ttgtgccaat gatgtctcag gcggacaagt
     2701 gttcgggcag gatcaaaatg cgttgcattt gttctggaaa aatggtgaaa aaacgcttcc
     2761 tctggcggat aaaagcaaat tggcagaaag tttggtacag gaaattgtaa aacattatcg
     2821 agaaaaataa tgaaattact ggttagccat tttcatcaag gcgaagggaa atttcctaca
     2881 ttctcagctg gtactgccgt tcgattaggg agtgaatgtg taaactaccc aaactggttt
     2941 gcctgtgaaa ttgacggttg gaaaaccttt gtgccaagcc attttattag cgaagataaa
     3001 ttgatatgcg attataaccc aacggaactt gttgcagcac aaggcgagag agtagaattc
     3061 cttgaactac attaccaatg ggcaattgtc aaaagagaaa acgaaattgg ctggctacct
     3121 tgtgaaatct taagggtaat aggatgaaaa ttatgcaact tacgtcaaat cagtatatca
     3181 acgacattaa acatattctt ttatctgctc gccaaaaggc gtatcaagcc gtgaattcgg
     3241 caatggtcga agcctattgg aaaatcggtg aacgaattgt gcaagaagag aaaaatatgg
     3301 tcaaagatca aaatgaaaaa gtataatatt gtatt
//
LOCUS       DANLZC010000190         3323 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.190, whole genome shotgun sequence.
ACCESSION   DANLZC010000190 DANLZC010000000
VERSION     DANLZC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.190"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(47..670)
                     /locus_tag="PXE44_002403"
     CDS             complement(47..670)
                     /locus_tag="PXE44_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5589862.1"
                     /translation="MQERITQQNERLSAYMDGQNVDTAFVETLTNSPELQQKWASYHT
                     IRSVMQGDEIILGRDFSAKMEALLENEEIESQANVEKPKGLLLKLKRWGTPIMQAGIA
                     ASVCLVAVLGVNSMNASDEIAQTQQPVLQTLPFSNSVEAVSYNAPVQDQPTEEQLELQ
                     QRKINALLENHELQRRTNNVKNLTLSEEEKQKAQTSSNTESEPNIQK"
     gene            complement(716..1288)
                     /gene="rpoE"
                     /locus_tag="PXE44_002404"
     CDS             complement(716..1288)
                     /gene="rpoE"
                     /locus_tag="PXE44_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDL5589863.1"
                     /translation="MSEQITDQELVEKAQKGDKKAFNLLVVRYQNRVAGLLTRYVARD
                     DIPDIVQESFIKAYRSLESFRGESAFYTWLYRIAVNTAKNHLTSLGRRPPKEDILAED
                     AESYDVGAQLRESDTPENLILSEELKKVVFDTIENLPDELKTAIRLRELEGLSYEEIA
                     EVMECPVGTVRSRIFRAREAIDAKIKPLMQ"
     gene            complement(1510..3054)
                     /gene="leuA"
                     /locus_tag="PXE44_002405"
     CDS             complement(1510..3054)
                     /gene="leuA"
                     /locus_tag="PXE44_002405"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDL5589864.1"
                     /translation="MSNNNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVM
                     EVGFPVSSAGDFESVQTIARHIKNSRVAALSRAVAKDIDAAYEALKVAEAFRIHTFIA
                     SSALHVEAKLKRSFDDVVEMAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAI
                     NAGATTVNIPDTVGFCLPNEYGNIIHQVMNRVPNIDKAIVSVHCHNDLGMATANSLTA
                     VQNGARQIECTINGIGERAGNTALEEVVMAIKTRQEFMGVDTRINTQEIHRVSQMVSQ
                     LCNMPIQPNKAIVGSNAFAHSSGIHQDGMLKNKNTYEIMSPETIGLKKEKLNLTARSG
                     RAAVKGHMADMGYTETDYDLDKLYEAFLQLADKKGQVFDYDLEALAFIDMQQGDEDRL
                     KLDVITTQLISGLPASAFVQVELDGVRQSATSQGGNGPVEATYNAIMNIVGMELKMSH
                     YNLTAKGEGAEALGQVDIVVEYEGRKFHGVGLATDIVESSARALIHAINAIYRSQKVA
                     DLKAKK"
BASE COUNT          901 a          725 c          590 g         1107 t
ORIGIN      
        1 ataattatgg ataaattatg tggctttcag taacttattt tacctattat ttttgtatat
       61 ttggctctga ttctgtattg gaagaagttt gtgctttttg cttttcttcc tcagataagg
      121 ttaaattttt aacgttatta gtacggcgtt gtaactcatg gttttctaat aacgcattaa
      181 tcttacgctg ttgaagctct aactgttctt ctgttggttg atcttgaact ggcgcattat
      241 aacttaccgc ttcaactgaa ttgctaaatg gcaatgtttg taatactggt tgttgcgttt
      301 gagctatttc atcactcgca ttcattgaat taacccctaa taccgcaact aagcacactg
      361 aagctgcaat accggcttgc ataattggag ttccccaacg cttgagcttg agcaataagc
      421 ctttaggttt ctctacattt gcttgagatt caatttcttc attttccagt aatgcttcca
      481 ttttagcaga gaaatctctc cctaaaatga tctcatcacc ttgcatcaca ctacgaatag
      541 tgtgataact tgcccatttt tgctgtaatt cagggctgtt agttaatgtt tctacaaatg
      601 ctgtatcaac attttgccca tccatataag ctgaaaggcg ttcattttgt tgagtgatgc
      661 gttcttgcat aattgactcc aatgatttaa atctcttcta aaataatctt tctatctatt
      721 gcataagtgg cttgatttta gcatcaatcg cttcccttgc tcggaaaata cgggaacgta
      781 cagtaccaac cggacattcc atcacttctg cgatttcttc gtaacttagc ccttccaatt
      841 ctcttaaacg aatggcggtt ttaagctcat caggtaaatt ttcaatcgta tcaaacacga
      901 ctttctttaa ttcctcggat aatatcaaat tctctggcgt atcactttca cgaagttgcg
      961 cgcctacatc ataactttca gcgtcttcgg caagaatatc ttcttttggc ggtcttctgc
     1021 ctaaagaggt taaatgattt ttcgcagtat tcactgcaat gcgataaagc cacgtataaa
     1081 aagcactttc gccacggaaa gactctaaag aacgataggc tttaataaaa gattcttgca
     1141 caatgtccgg aatatcatct cttgcaacat aacgagtcag caaaccggct actctatttt
     1201 gataacgtac aacaagtaaa ttaaatgctt ttttatcacc tttttgtgct ttttcgacta
     1261 attcttggtc ggtgatctgt tcgctcaact gcggactctc ctaaatatca atatgtcatt
     1321 ttccattgca cactttgtta gtgtgtgctt tatgacaaaa gttcaaaaaa tttgaaaaat
     1381 tgtgtaattt tttacattcc tagataaaac ctacttaata aactaagcaa gcggtcggat
     1441 tttagcgaaa ttttgcaaaa atttcaaaaa atccgaccgc ttgtctatgt tatgctttcg
     1501 ctctataagt tatttcttcg cttttaaatc tgccactttt tgtgaacggt aaatcgcatt
     1561 aatggcgtga attaaagctc gggctgaaga ttccacaata tcggttgcca aacccacacc
     1621 gtggaatttt cgaccttcat attccactac aatatccact tgccctaatg cttctgctcc
     1681 ttcgcctttc gcagtcagat tatagtgcga catttttagc tccattccca caatgttcat
     1741 aatggcattg taagttgcct caactggacc atttccccct tgagatgtag cactttgacg
     1801 aacgccgtct aattccacct gtacaaaagc cgaagccggt aaaccgctga ttagttgggt
     1861 ggtaattaca tctaatttta agcggtcttc atcgccttgc tgcatatcaa tgaaggcaag
     1921 agcttctaag tcataatcaa acacttgccc ttttttatca gcaagttgca agaaggcctc
     1981 ataaagttta tctaaatcgt aatccgtttc cgtataaccc atatcagcca tatgcccttt
     2041 caccgctgct ctacctgaac gggcagttaa attcagcttc tcttttttca agccgatggt
     2101 ttccggcgac ataatttcat aagtattttt atttttcagc ataccgtctt ggtgaatacc
     2161 tgaagagtga gcaaaggcat tgctaccgac aatcgctttg ttcggctgaa tcggcatatt
     2221 gcaaagctgg ctgaccattt ggctgacacg gtgaatttct tgggtattaa tgcgggtatc
     2281 aacccccata aattcttggc gggttttaat tgccattacc acttcttcaa gagcggtgtt
     2341 accggcacgt tcgccaatac cgttgatagt acactcaatc tgtctcgctc cgttttgcac
     2401 cgctgtcaat gagttggcgg tagccatacc taaatcgtta tggcagtgaa cagagacaat
     2461 cgctttatcg atattcggaa cacggttcat cacttggtgg ataatattgc cgtattcatt
     2521 cggtaaacag aaaccgactg tatccggaat attcactgtg gttgcaccgg cattaatggc
     2581 agcttctaca atgcggcaga tgttatcaat tccagtacgc ccagcatctt cacaggaaaa
     2641 ctcaacatca tcagtataat ttctggcgcg ttttaccgct gcaaccgcca tttcgactac
     2701 atcatcaaat gaacgtttta atttagcctc aacgtgtaat gccgagctgg caataaaggt
     2761 atggatacgg aatgcttctg ctacttttaa ggcttcataa gctgcatcaa tgtcttttgc
     2821 aaccgctcgg gaaagcgccg ccacccggct gtttttgata tgacgagcga tggtttgtac
     2881 tgattcaaaa tcgcccgctg aagaaactgg aaaaccgact tccatcacat caacccctaa
     2941 acgttctaaa gcaagggcaa tttgcaattt ttcttttacg gttaaacttg cttttaatga
     3001 ttgctcacca tcacgtaaag tcgtatcaaa aataataatg ttattgttgc tcatagaagc
     3061 ctctttttag gtgtttttat ttgataaaaa aagcccgcat tttaaggtgc gggcaagaat
     3121 gtggatagtc catttacttt ttccaatcaa tctttatcct cgcacaaaat acgataacag
     3181 agataatcgg agtaaggtaa atttttccat gttgtgttgt atgtttatgt ttgcattgtt
     3241 attcagtagt tgaggtgtat attatcctta tccaaatcaa tgtcaatagc atttttctct
     3301 tcacgcaacc gtttaccagc cca
//
LOCUS       DANLZC010000191         3312 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.191, whole genome shotgun sequence.
ACCESSION   DANLZC010000191 DANLZC010000000
VERSION     DANLZC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.191"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..332
                     /gene="metQ"
                     /locus_tag="PXE44_002406"
     CDS             <1..332
                     /gene="metQ"
                     /locus_tag="PXE44_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine ABC transporter membrane-anchored
                     lipoprotein MetQ"
                     /protein_id="HDL5589865.1"
                     /translation="TNLFSTSVDIIENPKNLKIKEVDTSIAAKALDDVDLAVVNNTYA
                     GQVGLNTQDHGVFVESKDSPYVNIIVARQDNKDAANVQNFIKSYQTEEVYQEAQKHFK
                     DGVVKGW"
     gene            415..1245
                     /locus_tag="PXE44_002407"
     CDS             415..1245
                     /locus_tag="PXE44_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL5589866.1"
                     /translation="MNFKKLLGVALVSALALTACKDEKAQAPATTAKTENKAPLKVGV
                     MTGPEAQMTEVAVKIAKEKYGLDVELVQFTEYTQPNAALHSKDLDANAFQTVPYLEQE
                     VKDRGYKLAIIGNTLVWPIAAYSKKIKNISELKDGATVAIPNNASNTARALLLLQAHG
                     LLKLKDPKNVFATENDIIENPKNIKIVQADTSLLTRMLDDVELAVINNTYAGQAGLSP
                     DKDGIIVESKDSPYVNLVVSREDNKDDPRLQTFVKSFQTEEVFQEALKLFNGGVVKGW
                     "
     gene            1297..2088
                     /gene="metQ"
                     /locus_tag="PXE44_002408"
     CDS             1297..2088
                     /gene="metQ"
                     /locus_tag="PXE44_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDL5589867.1"
                     /translation="MKIMKLAGAVAIFSLFLTACNDKAEKLKVGVISGPEHKVMEVAA
                     KIAKEKYNRDVELVVFTDYATPNAALDKGDLDLNAFQHKPYLDNQIQEKGYKLVPVGN
                     TFVYPIAAYSKKIKSLAELKDGDTIAVPNDPTNLARALILLEKQDLIKLRADAGLKAT
                     SVDIIENPRKLVIQEIEAPLLPRTLDDVAFSIINTTYAGQNGLTPTKDGIFVEDKDSP
                     YVNLIVARENNQHSEAVKDLVKAYQTEEVYNKANEEFKGAMIKGW"
     gene            complement(2133..3284)
                     /gene="metK"
                     /locus_tag="PXE44_002409"
     CDS             complement(2133..3284)
                     /gene="metK"
                     /locus_tag="PXE44_002409"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL5589868.1"
                     /translation="MAINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACET
                     YVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDAHSCAVLNAIGKQSPD
                     INQGVDRADPLEQGAGDQGIMFGYATNETEVLMPAPITYAHRLMEQQSKVRKSGKLDW
                     LRPDAKSQLTFAYENNKIIGIDAVVLSTQHAEHISQKELIEGVMEEIIKPVLPSEWLS
                     QNTKYFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGKVSNEILVKLIYQNF
                     DLRPYGLIKMLDLIKPIYRETAAYGHFGRKHFPWEKTDKAEALRAGAGL"
BASE COUNT         1037 a          634 c          703 g          938 t
ORIGIN      
        1 ataccaattt attctcaact tcagtggata ttatcgaaaa tccgaaaaac ttaaaaatta
       61 aagaagtgga tacttcaatt gccgcaaaag cgttagatga cgtagattta gcggtggtaa
      121 ataacactta tgccgggcag gtaggcttaa atacccaaga tcacggtgta tttgttgagt
      181 ctaaagattc accgtatgtg aatattattg tggctcgcca agacaataaa gatgcagcta
      241 atgtacaaaa ctttattaaa tcttaccaaa ccgaagaagt gtaccaagaa gcacaaaaac
      301 actttaaaga tggtgtagta aaaggttggt aatttagctg atttttactt aaatggcgat
      361 taagcgctag gttaatcgcc attttcatct ttaaaatata ggaaggagca aacaatgaac
      421 tttaaaaaat tattaggtgt ggcgttagta tctgccttag cacttactgc gtgtaaagat
      481 gaaaaagcac aagcacctgc tacaacagct aaaactgaaa acaaagcccc attaaaagtg
      541 ggtgtgatga ccggccctga agcccaaatg accgaagtgg cagtgaaaat tgcaaaagag
      601 aaatatggct tagatgtaga gttagtgcag tttactgaat acactcaacc aaatgccgca
      661 cttcattcta aagatttaga tgctaacgcg ttccaaacgg tgccttattt agagcaagaa
      721 gtgaaagatc gtggttataa attagcgatt atcggcaata cgctagtatg gccaatcgcg
      781 gcttattcta aaaaaattaa aaacatttcc gagttaaaag acggagcgac tgttgcgatt
      841 ccaaacaatg caagtaatac tgctcgtgcg ttattattgc ttcaagctca cggtttattg
      901 aaattaaaag atccgaaaaa tgtgtttgct accgaaaacg atattatcga aaacccgaaa
      961 aatatcaaaa tcgtacaggc ggatacctca cttttaaccc gtatgttaga tgatgtagaa
     1021 cttgcggtaa tcaacaacac ttacgcaggt caagctgggt taagtccgga taaagacggt
     1081 attattgtgg aatctaaaga ttcaccgtat gtgaatttag tggtaagtcg tgaagataat
     1141 aaagatgacc cacgcttaca aacttttgtg aagtcattcc aaaccgaaga agtattccaa
     1201 gaagcgttaa aattatttaa cggtggtgtg gtgaaaggtt ggtaatcagt tggacggcat
     1261 attctgccgt ccttttctta aaaggagaaa cagataatga aaataatgaa attagccggt
     1321 gcagttgcaa ttttctcgct ttttttaacc gcttgtaatg ataaagccga aaagttgaaa
     1381 gtcggtgtga tttccggccc tgaacataag gtaatggaag tggcggcaaa aattgcaaag
     1441 gaaaaatata accgtgatgt tgaattagtg gtatttaccg attatgccac gcctaatgca
     1501 gctttagata aaggcgatct tgatttgaat gctttccagc ataaacctta tttagataac
     1561 caaattcagg aaaaaggcta taaattagtg ccggtcggca atacttttgt ttatccgatt
     1621 gcggcttatt ccaaaaaaat taaatcgctg gcagagttga aagatggtga tactattgca
     1681 gtgcctaatg atccgactaa tttagcccgt gctttaattt tattggaaaa acaagattta
     1741 attaagctgc gagcagatgc aggcttaaaa gcaaccagtg tggatattat tgaaaaccct
     1801 cgtaaattgg tgatccaaga aattgaagca ccattattgc ctcgaacgtt ggacgatgtt
     1861 gccttttcga ttattaatac tacctacgca gggcaaaacg gtttaacgcc aaccaaagac
     1921 ggaatattcg ttgaagacaa ggattcgcct tatgtgaatt taattgttgc ccgtgaaaat
     1981 aaccaacatt ctgaagccgt aaaagatttg gtgaaagcct accaaacaga agaggtgtat
     2041 aacaaagcga atgaagagtt taaaggggcg atgataaaag gctggtaatt gaagataaat
     2101 aaggcataga ttcctatgcc ttacttttta tgttacagac ctgctccggc tcttaatgct
     2161 tcggctttgt cagttttttc ccaaggaaaa tgtttacgac cgaagtgtcc gtaggcagcg
     2221 gtttcacggt agattggttt gattaaatct aacattttga ttaagccata cgggcgtaag
     2281 tcaaagtttt ggtagattaa tttcacaagt atttcgttgc tcactttacc tgtgccgaag
     2341 gtttcgacca taattgatgt tgggtcagcc accccgatag cataagaaag ttggatctca
     2401 cagcgatcgg ctaagcctgc ggcaacgata ttttttgcga cataacgagc agcataagca
     2461 gcagagcgat ccacttttga cgggtcttta ccagagaatg caccaccacc gtggcgagcc
     2521 gcaccaccgt aggtatctac aatgatttta cgcccggtta aaccacagtc acccattgga
     2581 ccgccaatca caaaacgacc ggttgggttg atgaaatatt tggtgttttg gctgagccat
     2641 tcgcttggta gtaccggttt gatgatctct tccatcaccc cttcaattaa ctctttttgc
     2701 gaaatatgct ctgcgtgttg tgtagaaagc actacggcat caatgccgat aattttattg
     2761 ttctcataag caaatgttaa ttggctttta gcgtcagggc gtaaccaatc gagtttgccc
     2821 gatttacgca cttttgactg ctgttccatt aagcggtgtg cgtaagtaat tggagccggc
     2881 attaacacct cggtttcatt ggtagcgtag ccaaacataa tcccttggtc gcccgcacct
     2941 tgctctaacg gatcggcacg gtcaacgccc tggttgatgt ccggagattg tttaccaatc
     3001 gcatttaata ccgcacaaga gtgagcatca aagcccatat cagagtgagt atagccgatg
     3061 tcgcaaatca cttggcgggt taagttttca atatccaccc acgcagaggt agtgatttct
     3121 ccccctacta atgccatacc ggttttcaca taagtttcac aagcaacgcg tgctttaggg
     3181 tcttgtttta aaatttcatc taataccgcg tctgaaattt ggtctgcgat tttatccgga
     3241 tgcccttctg aaaccgattc agaagtaaat aaattaattg ccatttgtat tcctctctat
     3301 cttaaatatg ga
//
LOCUS       DANLZC010000192         3307 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.192, whole genome shotgun sequence.
ACCESSION   DANLZC010000192 DANLZC010000000
VERSION     DANLZC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.192"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(92..214)
                     /locus_tag="PXE44_002410"
     CDS             complement(92..214)
                     /locus_tag="PXE44_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HDL5589869.1"
                     /translation="MKKLVLTILAAATFLTACNTVDGVGEDVEKAGDKIQQAAQ"
     gene            complement(294..1271)
                     /gene="rluD"
                     /locus_tag="PXE44_002411"
     CDS             complement(294..1271)
                     /gene="rluD"
                     /locus_tag="PXE44_002411"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDL5589870.1"
                     /translation="MTQQKILTAEITADLLGARLDQALAQLFPDYSRSRIKVWIESDL
                     VKVNGNIVNKAREKVFGGETVEITAEIEEEIRFEPQDIPLNIVYEDDDIIVINKPKDL
                     VVHPGAGNPDGTVLNALLHYYPPIAEVPRAGIVHRLDKDTTGLMVVAKNIPAQTHLVT
                     ALQKRRITREYEAIASGIMTQGGKVDEPMARHPTKRTAMAVHPMGKPAVTHYRIMERF
                     RNYTRLRLRLETGRTHQIRVHMAHIAHPLLGDQLYGGRPRPPKGASDSFLSVLRGFQR
                     QALHATMLRLEHPITGEMMEWHAPLPDDFIELIQALKADYELYKDQLDY"
     gene            1412..2191
                     /locus_tag="PXE44_002412"
     CDS             1412..2191
                     /locus_tag="PXE44_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDL5589871.1"
                     /translation="MRKFKSLATLVLAGLFVAGCSNSNKELEQSNVQDLYGKGQTYLQ
                     DGDYNSAIRYLEAVGTKGGQYSGLGEQTQLSLIYAQYKVGEYYKALDAAERFARSYPN
                     AANMDYVFYLAGLSNARLSDNFIQDFFGVNRASRAVDNVRNAYGNFQTIVQRYPQSQY
                     AQDAQNWMNYLFNRLAEHELAVVKFYDERNAYVAVVNRIEEMLRFYPNTLATKQALPY
                     LKEAYKQMNIPDAEQKTELLIQEYESKELPNPEKLKYGEQF"
     gene            2264..3295
                     /gene="tsaD"
                     /locus_tag="PXE44_002413"
     CDS             2264..3295
                     /gene="tsaD"
                     /locus_tag="PXE44_002413"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL5589872.1"
                     /translation="MRILGIETSCDETGVAIYDEDKGLVANQLYSQIDMHADYGGVVP
                     ELASRDHIRKTLPLIQEALKEANLQPSDIDGIAYTAGPGLVGALLVGSTIARSLAYAW
                     NVPALGVHHMEGHLLAPMLEENAPEFPFVALLISGGHTQLVKVDGVGQYELLGESIDD
                     AAGEAFDKTGKLLGLDYPAGVAMSKLAESGTPNRFKFPRPMTDRPGLDFSFSGLKTFA
                     ANTIKANLNENGELDEQTKCDIAHAFQQAVVDTILIKCKRALEQTGYKRLVMAGGVSA
                     NKQLRADLAEMMKKLKGEVFYPRPQFCTDNGAMIAYTGFLRLKNDEQTDLSISVKPRW
                     AMTELPPIN"
BASE COUNT          979 a          713 c          651 g          964 t
ORIGIN      
        1 tttaccgtat ttttcgcaaa aatatacctt attgaacaat tgttcaataa ggtatatata
       61 ttattagctt cttagaattc gaagcttttt gttattgtgc agcctgctga attttatcgc
      121 ccgctttttc aacatcctca ccaacaccat caacggtatt acacgcagtc aagaaagttg
      181 cagctgctaa aatagttaat actaattttt tcatagaaag ctcctttttt gttgtttact
      241 tatgaaaacg atttcaatat agcaacttta cattatttaa gcaacaccta aacttaataa
      301 tctaattgat ctttataaag ctcataatcc gcttttagtg cctgaatcaa ttcaataaaa
      361 tcatcaggta acggggcgtg ccactccatc atttcaccag taatcggatg ctctaatctc
      421 aacattgtgg cgtgtaaggc ttggcgttgg aagcctcgta acaccgataa aaacgagtca
      481 ctcgcacctt taggtgggcg aggtctaccg ccatagagct gatcacctaa taatgggtga
      541 gcaatatgcg ccatatgtac ccgaatttgg tgagtacgcc ctgtttctaa tcttaaacgt
      601 aaacgtgtgt aattacgaaa acgctccata atacgataat gcgtaaccgc cggttttccc
      661 attgggtgaa cagccattgc cgtacgttta gttgggtggc gagccattgg ctcatcaact
      721 tttccgccct gtgtcataat gccgctggca atcgcttcat attcacgggt aatacgacgt
      781 ttttgtaatg cggttactaa gtgagtctgt gccggaatgt ttttagccac taccattaag
      841 cctgtcgtgt ctttatccaa tcggtgaaca atccctgctc gaggcacttc tgcaatcggt
      901 ggataataat gtagcaaagc attcagcact gtaccgtccg gattaccagc ccctgggtga
      961 accactaaat ccttcggttt attaatgacg ataatatcgt catcttcata cacaatattt
     1021 agaggaatat cttgaggctc aaaacgaatt tcctcttcta tttcagcggt aatttcaaca
     1081 gtttcaccac caaatacttt ttcccttgct ttattcacga tattgccatt aactttcact
     1141 agatcacttt caatccacac tttaatgcgt gaacgagaat agtccggaaa aagttgtgcc
     1201 agagcctgat ctaggcgagc acctagtaaa tctgcagtaa tttctgctgt taaaatcttt
     1261 tgttgagtca ttaaaccata aatcctaatt agtcatttgc ttacttgcgt gatgttatat
     1321 agaatagcga gatgaacttc ataagttatt cttagtcaga ggacattgta cattaacttt
     1381 tcgtcctaga cactttttat aggtaaaaca tatgcgtaaa tttaaatctc ttgccacatt
     1441 agtattagcc ggactgtttg ttgcaggttg ttcaaattca aataaagaat tagaacaatc
     1501 caacgttcaa gatctttatg gcaaaggtca gacctattta caagatggag attataattc
     1561 tgccattcgc tacttagaag cggttggaac caaaggaggc caatattcag gacttggtga
     1621 gcaaacccaa ctaagtttaa tttatgctca atataaagtt ggcgaatact ataaagcact
     1681 tgatgccgca gaacgttttg ctcgctctta cccgaatgca gctaatatgg attacgtatt
     1741 ctatctagca ggattatcaa atgcacgtct aagtgataat tttattcaag acttctttgg
     1801 tgtaaatcgc gcctctcgag ccgtagataa tgttcgtaat gcttacggta acttccaaac
     1861 tattgtacag cgctaccctc aaagccaata tgcacaagat gctcaaaatt ggatgaatta
     1921 cttatttaac cgtttagcgg aacacgaatt agccgttgtt aaattttatg atgaacgtaa
     1981 tgcctatgtg gctgtagtaa accgtatcga agaaatgttg cgtttttacc cgaatacact
     2041 ggcaaccaaa caagcattgc catacttaaa agaagcatat aagcaaatga atatcccaga
     2101 tgcagagcaa aaaacggaac tcttgatcca agaatatgaa agcaaagagc taccgaatcc
     2161 tgaaaaactg aagtatggcg aacaattcta gtcgtacaga gaataatgtg aggggcgttc
     2221 ttcgcccctt ttggttttct aacttatttt gacttctcca actatgcgaa ttttaggtat
     2281 tgaaacctct tgtgatgaaa ccggtgttgc catttatgat gaagacaaag gcttagtggc
     2341 aaaccagctt tatagccaaa ttgatatgca cgccgattac ggtggcgtag tccctgaact
     2401 ggcttctcga gaccatatcc gtaaaacgtt gccactaatt caagaagcct taaaagaggc
     2461 caatctgcaa ccctcggata ttgacggcat tgcctatact gccggcccag gcttggtcgg
     2521 ggctttattg gtcggctcaa ccattgcccg ttcgctggct tatgcttgga atgttccggc
     2581 attgggcgtt caccatatgg aagggcattt acttgcccca atgttggaag aaaatgcccc
     2641 tgaatttccg tttgtggcat tattgatttc aggtggacac acccaactgg taaaagttga
     2701 cggcgttggg caatacgaac tactcgggga atcaattgat gatgctgccg gtgaagcctt
     2761 tgacaaaaca ggcaaactac tcggtttgga ttaccctgcc ggtgtagcga tgtcaaaatt
     2821 agccgaatcc ggcacgccaa atcgttttaa attccctcgt ccaatgaccg acagaccggg
     2881 actggatttc agtttctccg gtttaaaaac ctttgctgcg aatacgatta aagccaatct
     2941 taatgaaaat ggtgaactcg atgagcaaac caaatgcgat attgcccacg cattccaaca
     3001 agccgtggtt gatactattt taattaaatg caagcgagcg ttagagcaaa ccggctataa
     3061 acgcttagta atggcaggcg gcgtaagtgc caataaacaa ttacgagcag accttgcgga
     3121 aatgatgaaa aaattaaaag gcgaagtatt ctaccctcgc ccacaatttt gcactgacaa
     3181 cggcgcaatg attgcctaca ctggctttct tcgcttaaaa aacgatgaac aaaccgactt
     3241 aagcattagc gtaaaacccc gctgggctat gaccgaatta ccaccgatta attaaccttt
     3301 caagcgg
//
LOCUS       DANLZC010000193         3273 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.193, whole genome shotgun sequence.
ACCESSION   DANLZC010000193 DANLZC010000000
VERSION     DANLZC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.193"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..591)
                     /locus_tag="PXE44_002414"
     CDS             complement(<1..591)
                     /locus_tag="PXE44_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589873.1"
                     /translation="MKKYLMLCSLWFLSACFNESYDFRGKEFEEPVQAILVESDKIYF
                     IGSTIDAIFDKTLIDNTKASDVIYFLNKLLQSPYGRKISNHLITLAIYDENIQIQYSP
                     QFIAKNLTMSDIQALQKLGFQFSHSKEYLWKSYAGLGGKITQFKNRKEILEKYKFKQP
                     IIAKAKSYQKSIEFSFDDTISVIAAPITIPASIILFV"
     gene            825..1115
                     /locus_tag="PXE44_002415"
     CDS             825..1115
                     /locus_tag="PXE44_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL5589874.1"
                     /translation="MALRPLHDKVILKREEVETKSAGGIVLTGSAATKSTRGKVIAVG
                     TGRILDNGSVQPLAVKVGDVVIFNEGYGVKTEKIDGEEVLILAEHDILAIVE"
     gene            1184..2824
                     /gene="groL"
                     /locus_tag="PXE44_002416"
     CDS             1184..2824
                     /gene="groL"
                     /locus_tag="PXE44_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608625.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL5589875.1"
                     /translation="MAAKDVKFGNDARVKMLKGVNILADAVKVTLGPKGRNVVLDRAY
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIVNE
                     GLKAVAAGMNPMDLKRGIDKAVAAVVEELKAISKPCETSKEIEQVGTISANSDSTVGQ
                     LIAQAMEKVGKEGVITVEDGTGLEDALDVVEGMQFDRGYLSPYFINKPEAGTVELENP
                     YILLVDKKVSNIRELLPVLEGVAKAGKPLLIIAEDIEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIAILTAGTVISEEIGMELEKATLEELGQAKRVVISKDNTTIID
                     GIGDETQIKGRVAQIRQQIEDSTSDYDKEKLQERVAKLAGGVAVIKVGAATEVAMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVALVRAANKVADTLKGDNEEQNVGIKLALRAMEA
                     PLRQIVANAGEEASVVARNVKEGSGNYGYNAGTEQYGDMLEMGILDPTKVTRSALQFA
                     ASIAGLMITTECMITDLPKEEKLDPAAMGGMGGMGGMM"
     gene            complement(2949..3236)
                     /locus_tag="PXE44_002417"
     CDS             complement(2949..3236)
                     /locus_tag="PXE44_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HDL5589876.1"
                     /translation="MVSSNAIASVKTSSLTVAKPTLSKNLPTECEKMFNTANKLVSDA
                     ERQPGTHTQVLKMKNKLSLTKQQILKMDSELQQKSCEKGLTALNSLKQKHQ"
BASE COUNT         1041 a          522 c          728 g          982 t
ORIGIN      
        1 tacaaataaa ataatagatg cgggaatcgt aattggtgct gcaatgaccg aaatggtgtc
       61 atcaaagcta aattctatgc ttttctgata acttttcgct ttagcgatta taggttgctt
      121 aaatttatac ttttctagta tttccttgcg atttttaaac tgtgtgattt taccaccaag
      181 acctgcatag cttttccata gatattcttt tgaatggcta aattgaaaac ctaacttttg
      241 tagagcttga atatcgctca tggtgagatt ttttgcaata aattgcggac tgtactgaat
      301 ttgaatattt tcatcataaa tggctaaagt aattaggtga ttactgatct tccttccata
      361 cggactttga agaagtttat tcaaaaaata aataacatct gaagcttttg tattatctat
      421 cagcgtttta tcaaatatcg catcaattgt ggaaccaatg aaataaattt tatctgattc
      481 aaccaaaatt gcttgtacag gttcttcaaa ttcttttcct ctaaaatcat aactttcgtt
      541 aaagcatgcg gagagaaacc atagtgagca gagcattaag tattttttca ttttattatc
      601 ctatcttgaa attaatagtt taaatggtat aagagaattg gggtttttct aaacagaaac
      661 agatttctta ggtatatagt tttatcaaaa atggtcaaat ttgtaaaaaa ttttacaaat
      721 ttgaccgctt gcacttgaaa ctctaaaatc tgcctttata ttgttaggcg ttacgcattt
      781 tgcgtgaagt tttatttatt catttttaag aaaaggaaca tattatggca cttcgtccat
      841 tacacgataa agttatttta aaacgtgaag aagttgaaac aaaatcggca ggcggtattg
      901 ttttaaccgg ttcggcagca acaaaatcaa cccgtggcaa agtaattgca gtaggaacag
      961 gtcgcatttt agacaatggt tctgttcagc ctttagcagt taaagtgggc gatgtagtga
     1021 tttttaacga aggctacggc gtaaaaaccg agaaaattga cggcgaagaa gtgctaattt
     1081 tagcagagca cgatattttg gcgattgtag aataatttcg ttctgcacaa gcggttaaaa
     1141 aagcaaaatt ttttacatat ttcaaataca aaggatttag aaaatggcag caaaagacgt
     1201 gaaatttggt aacgatgcac gcgtaaaaat gttaaaaggc gtgaatattt tagccgatgc
     1261 ggtaaaagta actttaggtc cgaaaggtcg taatgtggtg ttagatcgtg cttatggtgc
     1321 accaacgatt acaaaagacg gtgtgtcggt tgcccgtgaa atcgaattag aagataaatt
     1381 tgaaaatatg ggggctcaaa tggtgaaaga ggttgcctct aaagccaatg atgccgccgg
     1441 tgacggtaca actaccgcaa ctgtattagc tcaagcgatt gtaaacgagg gcttaaaagc
     1501 agtggctgcg ggtatgaacc caatggattt aaaacgtggt atcgataaag cggttgcagc
     1561 agtggttgaa gagttaaaag cgatttcaaa accgtgcgaa acctcaaaag aaattgagca
     1621 agtagggacg atttctgcaa actcagattc aacagtcggt caattaattg ctcaagcaat
     1681 ggaaaaagtg ggcaaagagg gcgtgattac tgttgaagac ggtacaggtt tagaagatgc
     1741 attagacgtg gttgagggta tgcaatttga ccgtggttac ttatcgccat actttatcaa
     1801 taaaccggaa gccggtacgg ttgagttaga aaacccatat attcttttag tggataaaaa
     1861 agtgtctaac attcgtgaat tattaccggt gttagaaggt gttgcaaaag caggtaaacc
     1921 gttattaatt attgccgaag atattgaagg cgaggcgtta gctaccttag tggtgaacac
     1981 tatgcgtggt atcgtgaaag tggcggcagt gaaagcacct ggctttggtg atcgccgtaa
     2041 agcaatgtta caagatattg cgatcttaac tgcaggtact gtgatttctg aagagatcgg
     2101 tatggagctt gaaaaagcaa cccttgaaga gcttggtcaa gcaaaacgtg tggttatcag
     2161 caaagataac accacaatta ttgacggtat tggtgatgaa actcaaatca aaggtcgtgt
     2221 agcacaaatc cgccaacaaa tcgaagattc aacttcagat tacgacaaag aaaaactaca
     2281 agagcgtgta gcaaaattag ccggtggtgt tgcggtaatt aaagtaggtg cggcaactga
     2341 agtggctatg aaagagaaaa aagaccgtgt tgatgatgct cttcacgcaa cccgtgctgc
     2401 ggtggaagaa ggtattgtac cggggggcgg agttgcttta gtgcgtgcag ccaacaaagt
     2461 ggctgataca ctaaaaggcg ataacgaaga gcaaaatgta ggtatcaaac ttgccttgcg
     2521 tgcaatggag gctccattac gccaaatcgt tgccaatgca ggtgaagaag cctcagttgt
     2581 agcccgcaac gtgaaagaag gttcaggcaa ctacggctat aacgcaggta cagagcagta
     2641 cggcgatatg cttgaaatgg gaattttaga cccaactaaa gtgactcgtt cagcacttca
     2701 attcgcggct tctattgcag gtttaatgat cactacagaa tgtatgatta ccgatctgcc
     2761 aaaagaagag aaattagatc ctgccgcaat gggcggtatg gggggaatgg gcggaatgat
     2821 gtaattgccc attgagtgaa caaaagctac tgaaattaat tcagtagctt ttttgttggt
     2881 aacaagtggt taaatttatg ctattttttg caaataaaaa accaagcaag taagcttggt
     2941 tttagcgttt attgatgttt ttgtttaagg ctgtttagtg ctgttaaacc tttctcacaa
     3001 cttttttgct gtaattcaga atccattttt aggatttgtt gctttgttaa agaaagttta
     3061 tttttcattt ttagcacttg agtatgagta ccaggctgtc tttctgcatc agagaccaat
     3121 ttatttgccg tgttaaacat tttctcacat tcagttggca aatttttact taaagtaggt
     3181 tttgcaactg taagtgatga ggttttaaca cttgctatag cattactgga aaccaatatg
     3241 gtacaaagtg ttattcctaa aattcttttt agc
//
LOCUS       DANLZC010000194         3244 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.194, whole genome shotgun sequence.
ACCESSION   DANLZC010000194 DANLZC010000000
VERSION     DANLZC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.194"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(57..629)
                     /gene="pdxT"
                     /locus_tag="PXE44_002418"
     CDS             complement(57..629)
                     /gene="pdxT"
                     /locus_tag="PXE44_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDL5589877.1"
                     /translation="MKIAILALQGAFSEHTDKLQQLGVESMEIRQLADLNQDFDGLIL
                     PGGESTVQGKLLRELGLLEPLRQKILNGLPTLGTCAGLILLAENLVNDDKKHLATLPV
                     TVKRNAYGRQLGSFLVEEEVKYLGKIPLTFIRAPYIESTGKDVEILAKISGKIVGVKY
                     QNQIGISFHPEVNNDLSFHRYFVEMCRKNK"
     gene            complement(629..1501)
                     /gene="pdxS"
                     /locus_tag="PXE44_002419"
     CDS             complement(629..1501)
                     /gene="pdxS"
                     /locus_tag="PXE44_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDL5589878.1"
                     /translation="MTNRYDLNRQLAQMLKGGVIMDVTTPEQACIAEEAGACAVMALE
                     RIPADIRAAGGVSRMSDPKMIKSIQAAVSIPVMAKVRIGHVTEAQILQAIEIDYIDES
                     EVLSPADDTFHINKRKFEVPFVCGAKNLGEALRRINEGAAMIRSKGEPGTGNVVQAVH
                     HLRKMKQEIARLVSHTTDELYHVAKELQVPFDLVQYVHQYQKLPVVTFAAGGVATPAD
                     AALMMQLGAEGVFVGSGIFKSGDPAKRARAIVQAVTNYNDPKLLAELSEDLGEAMVGI
                     NEQEIELLMAARGV"
     gene            1712..3115
                     /locus_tag="PXE44_002420"
     CDS             1712..3115
                     /locus_tag="PXE44_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL5589879.1"
                     /translation="MLTYTFNTQEGTALYEQLYRFLKNDIESNILIAGDKLPSKRAFA
                     KHLGISVMTVETAYQQLVAEGYVNTQPKKGFFVNALNLPEKKEAISVQLQKISSEQYE
                     KNKKWQADLTNRQTLAENFPFSVWTKLVREVLKTHQSELMERAESGGVWTLKFAIANH
                     LRAFRGMIVNPTQIIIGAGTEYLYGLLIQLLGLDKHYALTEPSYDKLRRIYHSYRLST
                     ATVSMPFSIDELNSKNVDVIHTSPSHHFPTGHVMPIAKRYELLAWVAQKAERYIVEDD
                     YDSEFRFVGQPIPALKSIDMLGRVIYMNTFSKTLSSTVRIAYMVLPPELLNRFQQELG
                     FYASTVSNFEQYILAEFIQQGYFEKHINRMRAYYQKKRDKLLKAFKQGNLAEKIAIKE
                     EHSGLHFIVQLNTTLTERQILQAAEEQGIKFVALSAYYQDKSQIQQNAFIIGYSNLKD
                     DQINYVAESLKKILDNE"
BASE COUNT         1015 a          645 c          589 g          995 t
ORIGIN      
        1 caaaactact ttttctaaaa aagtataaaa aataagaggc aatagcccct tttcttttac
       61 ttattttttc ggcacatttc cacaaaatag cggtgaaaac ttaaatcatt attcacttcg
      121 ggatggaagg atatacctat ctgattttgg tattttactc caacaatctt accgcttatc
      181 ttggctaaaa tttctacatc ttttccagtg ctttctatat aaggcgcacg aatgaatgtg
      241 agaggaattt taccgagata tttaacttct tcttctacta aaaacgagcc taattgcctt
      301 ccgtaagcat tacgtttcac tgttacaggc aaagtagcca aatgtttttt atcgtcgttt
      361 accaaatttt ccgcaagcaa aattaaaccc gcacaagttc caagcgttgg caagccattt
      421 aaaatttttt gacgaagcgg ctcaagcaag cctaattcac gtaataattt accttgaaca
      481 gtcgattcgc caccaggtaa aatcagacca tcaaaatctt ggtttaaatc agcaagttga
      541 cgaatttcca tactttcaac ccctagttgc tggagtttat cagtatgctc gctaaatgca
      601 ccttgcagag caagaatagc aattttcatt agacacctct tgctgccatt agcaattcaa
      661 tctcttgctc attaattcct accattgctt cacctaaatc ttcagaaagc tccgcaagta
      721 atttaggatc gttgtagttg gtaacagctt gtacaatggc ccgcgctcgt ttagcaggat
      781 cgcctgactt gaaaatacct gagccaacga agactccttc tgcacctaac tgcatcatca
      841 atgcagcatc ggcaggggtt gcaacacctc cagcagcaaa ggtgacaaca ggcaattttt
      901 ggtactgatg tacatattgt actaaatcaa atggtacttg gagctcttta gctacgtgat
      961 aaagttcatc tgtggtatgt gaaaccaagc gggcaatttc ttgtttcatt ttgcgaagat
     1021 gatgtactgc ttgcaccaca ttgcctgttc ctggctcgcc cttactacgg atcatagcag
     1081 cgccttcatt aatacgacgt agggcttcgc ctaaattttt tgctccacat acaaatggca
     1141 cttcaaattt acgcttgtta atgtggaagg tatcatctgc aggagaaagc acctcacttt
     1201 cgtcaatata gtcaatttcg atcgcttgca ggatttgtgc ttctgtaacg tgtccaatac
     1261 gtactttcgc cattacagga atggataccg ctgcttggat actctttatc atcttgggat
     1321 cactcatacg agaaacgcca ccagctgcac gaatatctgc aggaattctc tctagtgcca
     1381 ttactgcaca agctccagct tcttccgcaa tgcaggcttg ctcaggggta gttacatcca
     1441 taattacacc gccttttaac atttgagcaa gttgtctgtt taaatcatag cgattagtca
     1501 ttgtactttt tccttctaat tgttgaataa ttgaactttt cgtagtttcg attagctcgg
     1561 cattaagttt atttttatat tcgctggatg cgagtgttca tctaccaatt taaatacact
     1621 cagccaattt gagtgaggaa acagcatgta ttgtaattta atctgaactg tttaaaatga
     1681 ccattttatg tttttcttat catgtcagat tatgcttacc tacactttta acacacaaga
     1741 aggcaccgct ctctatgagc aactttaccg ttttctaaaa aacgacattg aaagcaacat
     1801 acttatagca ggggataaac tgccgtctaa acgggctttt gcaaagcatt taggcatttc
     1861 agtaatgaca gtggaaacag cttaccaaca gttagtagca gaaggttatg tgaatactca
     1921 gcctaaaaaa ggcttttttg tgaatgcttt aaatttacca gaaaaaaagg aagccatatc
     1981 agttcagcta caaaaaatca gttcagaaca gtatgagaaa aataaaaaat ggcaggcaga
     2041 tttaactaat cgacagactt tagcagaaaa ttttccgttt tcggtttgga caaaattagt
     2101 acgcgaagtg ctgaaaacac accaaagtga gctaatggag cgtgctgaga gtgggggcgt
     2161 atggacatta aaatttgcga tagccaatca tcttcgagcc tttagaggaa tgatcgttaa
     2221 tccaacacag attattattg gagcgggtac ggagtatctt tacggtttgc tcattcagct
     2281 tctaggctta gataaacatt atgcactaac tgagcccagt tacgataaat tgcgcagaat
     2341 ttatcacagt tatcgcttat caacagccac agtatcgatg ccattttcaa tcgatgagtt
     2401 aaattcaaag aatgtggacg tgatacatac ctcgccatca caccattttc cgacaggaca
     2461 cgtaatgccg attgccaaac gttatgagtt gttagcttgg gttgcacaaa aagctgaaag
     2521 atatattgta gaagacgact acgacagcga atttcgtttt gtaggacaac caattcctgc
     2581 attgaaatct attgatatgc tcgggcgagt tatctatatg aatacttttt ctaaaacgct
     2641 ctcttcaact gtacgaattg cctatatggt tttgccacca gaattactga atcgttttca
     2701 gcaggaatta gggttttatg cttctaccgt ttccaatttt gagcaatata ttttggcaga
     2761 atttattcaa caaggctatt ttgaaaagca tatcaatcga atgcgagcgt attatcaaaa
     2821 aaagagggat aaattactaa aagctttcaa gcaaggcaat ttagcagaaa agattgcaat
     2881 aaaagaagaa cattcaggct tgcatttcat tgtacagcta aacacaacat taacagaaag
     2941 gcaaattttg caggcagcag aagaacaagg tataaaattt gtcgcattgt ctgcttatta
     3001 tcaagataaa tctcaaattc agcaaaatgc ttttataatt gggtactcta atttaaaaga
     3061 tgatcagatt aattatgtag cagagagcct gaaaaaaatt ttagacaacg aatagcaagt
     3121 atcaaaactg ctttaatttt tagcaaattg ccttaaaaac acgcaagtgg taattatttt
     3181 agtgattttt gcaaaaaatt accaaattct ccttgcaagg cattttgttt tgcgtataat
     3241 gcat
//
LOCUS       DANLZC010000195         3239 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.195, whole genome shotgun sequence.
ACCESSION   DANLZC010000195 DANLZC010000000
VERSION     DANLZC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.195"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..844
                     /locus_tag="PXE44_002421"
     CDS             <1..844
                     /locus_tag="PXE44_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5589880.1"
                     /translation="AGVLFACINTLIPKLTSISPIDSNVIALVQYLAAFIFLVPSMNN
                     IGFFSSLKTEHFGQHCFRIFLSAIGIQCWTMALANQLPIWQGIALLMTSPLFVTIGSG
                     LFLKEKVDKKRWIATAMGFVGAMIILEPWSDHFEMIALLPIAAAFFWAGYSLMVKKLS
                     RYDSPTTMVAYLFILITPFNLLIALTNLSPSGFSLPSWSDFGFLILLGFFTALAQLAV
                     AKAYSVADASYIQPFDFIKLPLNVLAGWLVFNWVPPGKLWLGAAIIIAATIYITHAET
                     KQSN"
     gene            complement(923..1414)
                     /gene="ilvN"
                     /locus_tag="PXE44_002422"
     CDS             complement(923..1414)
                     /gene="ilvN"
                     /locus_tag="PXE44_002422"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDL5589881.1"
                     /translation="MRRTLAVLLENESGALSRVVALFSQRGFNIESLTVAPTDDPSLS
                     RMTIVAQGEEQVLEQIEKQLHKLIDVFKVSNLSAYEHVEREVLLVKVRATGSSRDELK
                     RMVDIYRGQIVDLTPKLYTIQLSGSTEKLNAFIEAVKEETTIVEIVRSGVISLSRGDK
                     NAL"
     gene            complement(1407..3128)
                     /locus_tag="PXE44_002423"
     CDS             complement(1407..3128)
                     /locus_tag="PXE44_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDL5589882.1"
                     /translation="MKKLSGAEMIVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLGLNH
                     VLVRHEQGAVHMADGYARSTGKVGCVLVTSGPGATNTITGILTAYTDSVPLVILSGQV
                     PSSLIGTDAFQESDMIGISRPVVKHSFLVKNTEEIPEIIKKAFYIASTGRPGPVVIDI
                     PKDVVNPLNKYPYEYPQEVSMRSYNPTVQGHKGQIKKALKALLVAKRPVLYVGGGVIS
                     AEGSPEITEFAQKLNLPVTCSLMGLGAYPASDKQYLGMLGMHGTFEANNAMHESDLIL
                     GIGVRFDDRTTNNLAKYCPNAKVIHVDIDPASISKTVQAYIPIVGSAKDVMEEFLSLL
                     GEENLAKNQVDLTAWWQQIDAWKARNCLAFEENSDVIKPQQVIRMIHKLTNGEAYVAS
                     DVGQHQMFAALHYGFDKPRRWINSGGAGTMGFGLPAAMGVKFAHPDATVVCVTGDGSI
                     QMNIQELSTAKQYDTPIVIVSLNNRFLGMVKQWQDIIYSGRHSQVYMNSLPNFAKLAE
                     AYGHVGIQIDHPSELEAKLTEAFSIKDKLVFVDVLVDETEHVYPMQVRGGAMNEMMLS
                     KTERTDA"
BASE COUNT          892 a          698 c          609 g         1040 t
ORIGIN      
        1 tgccggtgtg ttatttgcct gtatcaatac cttaattccc aaattaacgt ccatttcacc
       61 gattgactcg aacgtgattg cattggtgca atatttagct gcctttattt tcctcgttcc
      121 cagcatgaat aatatcggtt tcttctcttc attaaaaacc gagcattttg ggcaacactg
      181 tttccgtatt tttctatctg cgattggtat tcaatgttgg acaatggcac tcgctaacca
      241 attacctatt tggcagggaa ttgcgttatt gatgacttct ccattgtttg tgaccatcgg
      301 ctccggtctg tttttgaaag agaaagtgga taaaaagcgt tggattgcaa ccgcaatggg
      361 gtttgttggg gcgatgatta ttctagagcc ttggagcgat cattttgaaa tgattgcgtt
      421 actgccgatt gccgcggctt ttttctgggc aggttattcg ctaatggtga aaaaactctc
      481 tcgttacgat agcccaacta cgatggtcgc ttacctgttt attctaatta cgccttttaa
      541 tttgttgatt gcactcacga accttagccc tagtgggttt agtctgcctt cttggagcga
      601 tttcggcttt ttaatcttac ttggtttctt tacggcgtta gcgcaactgg cggtagccaa
      661 agcctacagt gtagcagatg cttcctacat tcagccgttt gattttatta aattaccttt
      721 gaacgtatta gccggttggc ttgtatttaa ctgggtgcca ccgggtaaat tatggctagg
      781 agctgcgatt attattgctg cgaccattta tattactcac gctgaaacta aacagagtaa
      841 ttagcaacaa attcacgcta cataaaaatc cctttatctc aaagataaag ggatttgcaa
      901 attttaagct aaaattgacc gcttacagag cgtttttatc gccacgagat agactaatca
      961 cgccagagcg aacaatctct acaatcgttg tttcttcttt aactgcctca ataaatgcat
     1021 tgagcttctc ggtagaaccg gataattgaa tggtgtaaag tttcggtgtt aaatctacga
     1081 tttgcccacg gtaaatatct accatacgtt tcagttcatc acgagatgaa cctgtagctc
     1141 gcacttttac caataacact tcacgctcaa cgtgttcata agcacttaag ttactcactt
     1201 taaacacatc aatgagtttg tgtagttgct tttcgatttg ttctaacacc tgttcttcgc
     1261 cttgcgcgac aattgtcata cgggaaaggc ttggatcatc agtaggggca acggtcaagc
     1321 tttcaatgtt aaaaccacgc tgcgagaaaa gggcaactac acgagataat gcacctgatt
     1381 cattttctaa taatacggct aatgttctac gcatctgttc tctccgtttt acttaacatc
     1441 atttcattca ttgctcctcc acgcacttgc attgggtaaa catgttccgt ttcatcgacc
     1501 aatacatcaa caaatacaag cttatcttta attgagaatg cctcagttaa ttttgcctcc
     1561 aattcagaag ggtggtcgat ttgaatccca acgtggccat aagcctctgc taatttagca
     1621 aagttaggta gagaattcat atacacctgt gaatggcggc cagagtaaat aatatcttgc
     1681 cactgtttca ccatacctaa gaaacggtta tttaagctga caatcacaat cggtgtgtcg
     1741 tattgctttg cggtggaaag ttcctggata ttcatttgga tactaccatc accggttaca
     1801 caaacaacag tggcatcagg gtgggcaaat tttaccccca tcgcagcagg taagccaaag
     1861 cccattgtgc ctgcaccgcc tgaattaatc caacgacgtg gtttatcgaa cccataatgc
     1921 aatgcagcaa acatttgatg ttgtccaaca tctgaggcaa cataagcctc cccattggtc
     1981 aacttgtgga tcattctaat cacttgttgt ggcttaatca catcactgtt ttcttcaaag
     2041 gctaagcaat tacgagcttt ccaagcatca atttgttgcc accaagcggt taaatctacc
     2101 tgattttttg caagattttc ctcacctaat aaagataaaa attcttccat tacatcctta
     2161 gcactgccta caattggaat ataagcttgt actgttttgg aaattgaggc tggatcaata
     2221 tccacgtgga tcacttttgc attcgggcaa tattttgcta agttatttgt tgtacgatca
     2281 tcaaaacgta ccccaatacc taaaatcaaa tcactttcgt gcattgcatt atttgcctca
     2341 aaagtgccgt gcatgcctaa catacctaaa tattgcttat cagaggcagg ataagcacct
     2401 aatcccatta atgaacaggt tacaggtaga tttagctttt gtgcaaactc tgtaatttct
     2461 ggcgaaccct ctgcactaat tacaccaccg cctacataaa gcactggacg ttttgctact
     2521 aatagtgctt ttaatgcttt tttaatttgc cctttatgcc cttgaactgt tgggttataa
     2581 gaacgcattg atacttcttg cggatattca tagggatatt tatttaatgg attgactaca
     2641 tctttaggaa tatcaataac taccgggcct ggtcgaccgg ttgatgcaat ataaaaggct
     2701 tttttgataa tttcaggaat ttcttctgta ttttttacta agaaactgtg ttttacaacc
     2761 ggacgagaaa tgccgatcat atcactttct tggaatgcat ctgtcccaat taaacttgat
     2821 ggaacttggc ccgacagtat tactaacgga acagagtcgg tataagcagt taaaatacca
     2881 gtaattgtat tcgttgctcc cggacctgaa gtcactagca cacagccgac tttacctgtt
     2941 gaacgggcat agccatcagc catatgtaca gcaccttgct cgtgtcgaac taaaacgtga
     3001 ttcaatccaa gtgtatgaat tgcatcataa atatcgagta cagagccacc cggatagccg
     3061 aaaacgtatt ctacgccttc atcctttaag gactgtacaa tcatttctgc accagaaagt
     3121 tttttcattg aatcctccaa caaaatagca ttttcttctc taagatggtt ggattttgca
     3181 gtaaaatttt attgatgtct agttcttatt atgggatcaa atgctagatt tgctagatt
//
LOCUS       DANLZC010000196         3190 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.196, whole genome shotgun sequence.
ACCESSION   DANLZC010000196 DANLZC010000000
VERSION     DANLZC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.196"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            1..170
                     /locus_tag="PXE44_002424"
                     /pseudo
     CDS             1..170
                     /locus_tag="PXE44_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            422..1612
                     /locus_tag="PXE44_002425"
     CDS             422..1612
                     /locus_tag="PXE44_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDL5589883.1"
                     /translation="MSTSQQLIQQTEHFGANNYLPLPIVISKAKGIWVEDPEGNRYLD
                     MLSAYSAVNQGHCHPKIVQALKDQADKVTLTSRAFYNDQLGIWYEKICKLANKEMALP
                     MNTGAEAVETAIKTARRWGYDVKGIAENQAEIIACVGNFHGRTMAAVSLSSDPDYKRG
                     FGPMLGGIKLVPYGDLEALKAAITPNTAAFLVEPIQGESGILIPPEGFLEQAYELCKE
                     NNVLFIADEIQAGLGRTGKLFATEWEGFEPDIYILGKALGGGVFPISCVLANRDILGV
                     FNPGSHGSTFGGNPLACAVSIASIDVLLEEKLAERSRELGEYFLAELKKIQNPIIKEV
                     RGRGLFIGLELHQPARPFCEKLKEKGLLCKETHDTVIRFAPPLVIEKFNLDWALEKIR
                     EVLA"
     gene            1687..>3190
                     /gene="nhaC"
                     /locus_tag="PXE44_002426"
     CDS             1687..>3190
                     /gene="nhaC"
                     /locus_tag="PXE44_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL5589884.1"
                     /translation="MLILQTFSKNQPLVKGGFMSNRTEITDQNIRIPFRYAILPVIIM
                     VSIMAVAIIKFEASPHVPLLIGAISAAVIAMAHGYRWETIEKGIYRGIRMALPAIVII
                     IMIGLTIGAWLGGGIVATMIYYGLKLISPSLFLVTISIICAIVTLAIGSSWSTMGTIG
                     VAAMGIGFSLGIPAPMVAGAVISGAYFGDKMSPLSDTTNLAAGITETNLFEHIRHMFI
                     TTVPAFLIALSVYFYFGMEFRAENLDLAKIEDIMQAMQEHFLISPWLLLVPLLVVVLV
                     AKKVPALPALAAGIILGWLCHVFVQGGDVSVAVITLHDGYKIESGHEMIDKLFNRGGI
                     ESMMYTISLTIVAMTFGGIAEQAGMLHSVVEKILTLAKSAGSLISATIVSAFITNVTT
                     SEQYISILLPGRMYIKAYRDKGLSSRNLSRALEDGGTVTSVLVPWNTCGVYAFSMLQV
                     SAFEYAPYAIMNWLIPLIAIGLATLNIKIERLPPEELAKEPEEITLPKRGC"
BASE COUNT          908 a          589 c          722 g          971 t
ORIGIN      
        1 gaattgagaa tttttggagc caagcaaaac gccatttgcg taaatttaac ggcataccta
       61 aagctcattt tgagctgtat ttgaaggaat gcgaatagcg ttttaatcac agtgacttaa
      121 aatctcaaat ttccatttta aaacaattgg ttaggggttg tttaagttag ttatctagga
      181 cagccctaaa ttttgtagtt ggaatttcaa cggagaaagt tatctataaa cttacaaaag
      241 ttatagaaaa tggaggcatt tttgcttaaa gtatgctatc tattcatatt aattgcatag
      301 ttaatcaaca actgaagaaa aattagaagt agatcataaa atcaacaaaa agtccgtgct
      361 ttttagctaa agattgttaa actcttttgt gttacaagtt tttaacataa aggagttttt
      421 tatgtctact tctcaacaac tcatccaaca aaccgaacat ttcggtgcca ataactacct
      481 acctctacct attgttatta gtaaagctaa aggcatttgg gttgaagatc ctgagggtaa
      541 tcgctatttg gatatgctta gtgcctactc agcggttaat caagggcatt gtcatccaaa
      601 aattgtacag gcactaaaag atcaagcaga caaagtaacg ctgacctctc gagcatttta
      661 taacgatcaa cttggtattt ggtatgaaaa aatctgcaaa ctcgctaata aagagatggc
      721 attaccgatg aatacgggtg cagaagcggt agaaacagcg attaaaactg ctcgccgttg
      781 gggttatgat gtgaaaggaa ttgcagaaaa tcaagcagaa atcatcgctt gtgttggcaa
      841 ttttcacgga cgaacaatgg ctgcagtatc tctctcgtct gatccggatt ataaacgtgg
      901 tttcgggccg atgttaggtg gcatcaaact tgttccatac ggtgatttag aagcattaaa
      961 agctgcgatt acaccaaaca cagcagcgtt tttggttgag ccaatccaag gtgaatctgg
     1021 cattcttatt ccacctgaag gtttcttgga acaggcttat gaactatgca aagagaataa
     1081 tgtgctgttt attgctgatg aaattcaggc aggtcttggg cgtactggca agttatttgc
     1141 aacagaatgg gaaggctttg agccagatat ttatattcta ggtaaagcac ttggtggcgg
     1201 ggtgttccca atctcttgtg tgttagcaaa tcgtgatatt ttaggcgtat tcaatcctgg
     1261 ctcgcacggt tcgacatttg gtggtaatcc attagcctgt gcggtatcta ttgcctcaat
     1321 tgatgtactg ttggaagaaa aacttgcaga gcgttcccgt gagttaggtg aatacttctt
     1381 ggcagagctg aaaaaaatcc aaaatccaat tattaaagaa gttcgtggac gtggactgtt
     1441 tattggcttg gagttacatc aaccagcacg cccattctgc gaaaaactca aagaaaaagg
     1501 cttactctgc aaagagaccc acgatacagt tatccgcttt gctccccctt tggtgattga
     1561 aaaattcaat ttggattggg cattggagaa aattcgggag gtgttggctt aattgtcatt
     1621 ttctctatat tttatcctgc caaatcgcct ttaacccttt ttggcaggaa gcaaaacagt
     1681 tgtaaaatgc tgattttgca aactttttcc aaaaatcaac cgcttgtaaa aggtggcttt
     1741 atgtcaaaca gaacagaaat aacagaccaa aatatccgaa tcccgtttcg ctatgctata
     1801 ttgcctgtga ttattatggt atcgattatg gcggtggcta tcatcaaatt tgaagcctca
     1861 cctcacgttc cgttgcttat tggggcaatt agtgccgctg tgattgcaat ggcacatggc
     1921 taccgttggg agacaattga aaaaggaatt tatagaggca ttcgtatggc tttaccagcg
     1981 attgttatca ttattatgat tgggctaacc atcggagctt ggctaggtgg tggaattgta
     2041 gctacgatga tttactacgg cttgaaattg atctctccgt cgctgttttt agtaactatc
     2101 tcaataattt gtgctattgt gacccttgcg attggtagct cgtggtctac aatgggaaca
     2161 atcggtgtag cggcgatggg gatcgggttt agtctcggca ttccggcacc gatggttgcc
     2221 ggtgcggtga tctcaggagc atatttcggc gataaaatgt cgccactgtc agacactacc
     2281 aatctggcag cagggattac cgaaaccaat ctgtttgaac atatccgcca tatgtttatc
     2341 actaccgtgc cggcgttcct gattgcgtta tctgtctatt tctattttgg tatggaattt
     2401 agagccgaaa atctcgatct tgctaaaatt gaagacatta tgcaggcaat gcaggaacat
     2461 ttcttgattt cgccttggtt gctattagtg cctttattag tcgtggtgtt ggtggcaaaa
     2521 aaagtgcctg ccttaccggc attagctgca gggataattc ttggttggct ctgccacgtt
     2581 tttgtgcaag gcggtgatgt cagcgtagca gtgattactc tgcacgacgg ctataaaatt
     2641 gagagcggcc acgagatgat tgataagctg ttcaaccgtg gtgggatcga gtcgatgatg
     2701 tacaccattt cccttacgat tgttgcgatg acttttggtg gcattgcaga gcaagctgga
     2761 atgttacatt cagtagtgga aaaaatcctt acccttgcaa aatcagcagg tagcttaatt
     2821 agtgctacga ttgtatcggc atttattacc aacgtaacga catctgagca atatatttct
     2881 attttattac caggcagaat gtatataaaa gcctataggg ataagggact aagttcgaga
     2941 aacctttctc gtgcattgga agacggagga acagttactt cggttcttgt accttggaat
     3001 acctgtggtg tttatgcgtt ctcaatgttg caagtgagtg cttttgaata tgcaccgtat
     3061 gcaataatga attggctcat tccactgatt gcgattggct tggcaacctt gaatatcaaa
     3121 attgagcgat tgccaccgga agaattagcc aaagaacctg aggaaattac tctgccaaaa
     3181 cgtggttgtt
//
LOCUS       DANLZC010000197         3139 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.197, whole genome shotgun sequence.
ACCESSION   DANLZC010000197 DANLZC010000000
VERSION     DANLZC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.197"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(24..1208)
                     /locus_tag="PXE44_002427"
     CDS             complement(24..1208)
                     /locus_tag="PXE44_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDL5589885.1"
                     /translation="MTSSNTIKQLDKDYIAQTYGRFDLALSHGKGCEVWDFEGNKYLD
                     FTSGIGVNSLGWADEDWLQAVTTQAGKLAHTSNLFFTEPASQLAQKLVAASGLKRAFF
                     CNSGAEANEGAIKTARKYSFDKYGSGRSTVLTLVNSFHGRTISTLASTGQEVFHKHFF
                     PFTEGFEYLPVNGIEALKARLETENVCGILLEVVQGEGGVCPLEADYLHAVQSLCQEK
                     DILFMIDEVQTGIGRTGTLFAYQQFDLTPGLVTLAKGLGGGLPIGAFLLGEKVEMTLG
                     KSDHGSTFGGNPVSCAAANVVIDKINAEFLAQVQQKGQLLQQRLASLPKVKSVSGLGL
                     MLGVEFEEGILAADIVAKCINKGVLFLTAKSKLRLLPPLMISEQQIEQGIRVLEDVLK
                     DS"
     gene            complement(1221..2075)
                     /gene="argB"
                     /locus_tag="PXE44_002428"
     CDS             complement(1221..2075)
                     /gene="argB"
                     /locus_tag="PXE44_002428"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDL5589886.1"
                     /translation="MQQQEINQFANLLEKSTACLVKWQGKIIVVKYGGNAMINEELKK
                     SVMADLLLLHRLGVKVVLVHGGGLEISQGVKLLGKEPQFINGLRVTDSDTINVVLQML
                     AGKVNKSLVALLKGKGIGLCGIDGAMLQCKKLEAEVDYGFVGDIVKVDPTVIEIALNA
                     GLIPVVSTVGVDENGQAYNINADTAASEIAIALQAEKLVSMTDIAGLLRDRFDESTLI
                     PEVEVSEVQSLIEQGIIAGGMIPKIACCTDFIRAGGSEANIIDGRIDHAILVEMFGGK
                     NGTRFYKK"
     gene            complement(2167..3108)
                     /gene="argC"
                     /locus_tag="PXE44_002429"
     CDS             complement(2167..3108)
                     /gene="argC"
                     /locus_tag="PXE44_002429"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDL5589887.1"
                     /translation="MSKYKIFVDGAVGTTGLRIHERLSQADDIELLTLPEEQRKDLNA
                     RVAKVAEADLTFLCLPDEASKELVANAPENTRICDTSTAHRVNPNWVYGFPELSGQRE
                     KIQNAKRVAVPGCHATGYIALVRPLVEKGILPADYPLSCHSLTGYSGGGKAMIADYEN
                     ENSPNAFNAPRVYGLTLQHKHLPEMQAVTQMQNPPIFTPVVADYYSGMLVTVPLQIFA
                     LQAVKSGEEIANLLADYYQTSRLIHVHPFQSLPEDGMLAANAYAGKDSLEIFIYGNST
                     QLLLAARFDNLGKGASGAAVQCMNIMLGRDEVEGLEL"
BASE COUNT          816 a          790 c          628 g          905 t
ORIGIN      
        1 atattcctac tgtttataat aactcaagaa tctttcaaga catcctctaa cactctgatt
       61 ccttgttcta tctgttgttc agagatcatc agcggtggga gtagccttaa cttgctttta
      121 gcggttaaaa acagcacacc tttgttgata cattttgcaa caatatcagc ggctaaaatg
      181 ccttcctcaa attctacgcc gagcattaaa cctaagcctg aaacggattt aacttttggt
      241 aagctcgcta agcgttgctg taacaactgc cctttttgtt gcacttgggc gagaaattcg
      301 gcgtttattt tatcgattac tacatttgca gcagcacagg aaacaggatt gccaccaaag
      361 gttgagccgt ggtcgctttt gcctaatgtc atttccactt tttcgcctaa caggaaagca
      421 ccaatcggca agccgccgcc caagccttta gcgagcgtga ctaaaccagg agttaaatca
      481 aattgttgat aagcaaaaag tgtgcccgtt cgcccaatgc cggtttgcac ctcatcaatc
      541 ataaataaaa tatctttttc ttggcacagg ctttgcacgg cgtgcagata atccgcctct
      601 aatgggcaaa caccgccctc gccctgcacc acctccagca aaatcccgca aacgttctca
      661 gtttccaagc gagctttcag tgcctcaatg ccatttacag gtaaatattc aaagccctct
      721 gtaaagggga aaaaatgctt gtggaatacc tcttgcccag tggatgcgag ggttgaaatg
      781 gttcgtccgt gaaaagagtt cacgagggtt aaaaccgttg aacgaccgga accgtatttg
      841 tcgaaactat atttgcgggc ggttttaatt gctccttcat ttgcctctgc ccctgaattg
      901 caaaaaaatg cccgttttag accgcttgca gccactaatt tttgggctaa ttggcttgcc
      961 ggttcggtaa aaaacaggtt tgaggtgtga gctaatttgc cggcttgtgt ggtaacagct
     1021 tgtagccaat cctcatccgc ccagcctaaa ctatttaccc caatgccact ggtaaaatca
     1081 agatacttat tgccctcaaa atcccacact tcacagcctt ttccgtgcga cagtgcaagg
     1141 tcaaatctgc cataggtttg ggcgatataa tccttatcta attgcttaat cgtattactg
     1201 cttgtcattt cactttcctg ttatttttta taaaaccgtg tgccgttttt gccaccgaac
     1261 atttctacca aaatggcgtg gtcgattcgc ccgtcaataa tatttgcctc agagccacca
     1321 gctctgatga aatcggtaca gcacgcaatt ttggggatca tcccaccggc aataatgcct
     1381 tgctcaatca gggactgcac ttcgcttact tccacttccg gaattaaggt gctttcatca
     1441 aagcgatctc gcaataaacc tgcaatatcg gtcatcgaga ccaatttttc cgcttgtaac
     1501 gcaatcgcaa tctcgctggc agcggtgtct gcattaatgt tataagcctg cccgttttca
     1561 tcaacgccca ccgtggaaac aaccggaatc aagcccgcat taagagcaat ttcaatcaca
     1621 gttggatcga ctttcacaat atcgcccaca aagccgtaat ccacctctgc ctctaatttt
     1681 ttgcattgca acatcgcccc atcaatacca cacagcccaa tgcctttccc ttttaataag
     1741 gcgacaaggc ttttattaac tttgcctgcc aacatttgca aaaccacgtt aatcgtatcg
     1801 ctatcggtaa cacgcaaccc gttgataaat tgcggttctt tacccaataa tttcacgcct
     1861 tgtgaaatct ccagcccgcc accgtgtacc agtaccactt ttacgcctaa acgatgaagt
     1921 aacagcagat ctgccatcac tgattttttc agttcctcat taatcattgc gttaccgcca
     1981 tatttcacca caatgatttt accctgccat ttcaccaaac aagcggtgga tttttctaat
     2041 aaatttgcaa attgattgat ttcttgttgt tgcatatttc tttctcttgt agggacgggt
     2101 cctgtgcccg tccgaatatg ggcgatgatt atcggacagg cacaggacct gtccctacat
     2161 ttatatttaa agctctaacc cctcaacctc atctcttccc aacataatat tcatacactg
     2221 cactgcggca cctgatgccc ctttgcctaa attgtcaaaa cgggcagcaa gtaaaagttg
     2281 ggtgctgttg ccgtaaataa aaatctctaa gctgtcttta cctgcataag cattcgcggc
     2341 aagcattccg tcttcgggta gagattggaa agggtgaacg tgaattagtc ggctggtttg
     2401 gtagtaatcg gcgagcaaat ttgcaatttc ttcgccggat ttgaccgctt gtaaggcgaa
     2461 gatctgcaat ggcacagtga ctaacattcc gctgtaataa tcagcaacca caggggtgaa
     2521 aatcggtggg ttttgcattt gggtaactgc ctgcatttca ggcaaatgtt tgtgttggag
     2581 agttaaaccg taaacacgag gggcattgaa agcatttggg ctgttttcat tttcatagtc
     2641 agcaatcatc gctttgccac cgcctgaata gccggttaag gaatgacaac ttaaagggta
     2701 atcggcgggc agaatccctt tttccaccag tggacgaacc aacgcaatat agcccgtggc
     2761 atggcaaccc ggcaccgcaa cacgcttggc attttggatt ttttcacgtt gtccgctcaa
     2821 ttccggaaaa ccataaaccc aattaggatt gacccgatga gcggttgaag tatcgcaaat
     2881 tcgggtattt tccggtgcat ttgctactaa ttcttttgag gcttcatccg gcaaacagag
     2941 gaaagttaaa tccgcctctg cgacttttgc cacacgggcg ttcaaatctt tccgctgctc
     3001 ttcgggcaag gttaataatt caatatcgtc cgcttggctt aaacgttcgt gaatgcgtag
     3061 cccggttgtt cctactgccc catcaacaaa aatcttgtat ttgctcattg aaaatccttc
     3121 tttgactttt tctgctcgg
//
LOCUS       DANLZC010000198         3134 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.198, whole genome shotgun sequence.
ACCESSION   DANLZC010000198 DANLZC010000000
VERSION     DANLZC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.198"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(43..330)
                     /gene="ybgE"
                     /locus_tag="PXE44_002430"
     CDS             complement(43..330)
                     /gene="ybgE"
                     /locus_tag="PXE44_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDL5589888.1"
                     /translation="MIHKLYNLTAKGLLKALSFILAAVLFATIWVNSTAFALSFGGKT
                     PYLAMLVFYGMAILWVHGIGFEIRAAIWKVIFLPLLGYLIVIPALWILLVK"
     gene            complement(330..428)
                     /locus_tag="PXE44_002431"
     CDS             complement(330..428)
                     /locus_tag="PXE44_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CydX/CbdX family cytochrome bd oxidase small
                     subunit"
                     /protein_id="HDL5589889.1"
                     /translation="MFYVTWVLGVLLAIFFATVITISIEKTGKFDE"
     gene            complement(443..1576)
                     /gene="cydB"
                     /locus_tag="PXE44_002432"
     CDS             complement(443..1576)
                     /gene="cydB"
                     /locus_tag="PXE44_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDL5589890.1"
                     /translation="MIDYEILRFIWWILIAVLLIGFAITDGFDMGVLNLLPIIGKSNV
                     ERRIMINTIAPHWDGNQVWLLTAGGAIFAAWPTVYATSFSGFYIAMVLVLAALFFRPV
                     GFEYRAKIDNPKWRSAWDWGLFIGGFVPSLVFGVAFGNLLQGVPFEFNNINQSQYTGS
                     FFGLLNPFALVCGIISLMMLTTQGATWLQMKTTDELHNNSRKVAQVTASIVLVAFILA
                     GVWLYFKDGFVITSELDHNAQSLLTNKTVAVQAGAWFRNYAEMPVLWVVPALVVVGAL
                     LTIIASKANRSGLAFFSSSLMIVGVILTAAVSMFPFIMPSITHPEMSLTVWDATASHT
                     TLTVMLFVAGIFVPLVLAYTIWSYIKMYGRLDASYLEENKAAY"
     gene            complement(1589..>3134)
                     /locus_tag="PXE44_002433"
     CDS             complement(1589..>3134)
                     /locus_tag="PXE44_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDL5589891.1"
                     /translation="DVVELSRLQFALTALYHFLFVPLTLGLSFVLVVMETLYVTTGKE
                     VYKDMTKFWGKLFGINFALGVTTGITMEFQFGMNWSYYSHYVGDIFGAPLAIEGLMAF
                     FLESTFVGLFFFGWERLSKAKHLLATYCVAFGSNFSALWILVANGWMQNPIASEFNFE
                     TMRMELSSFSELVLNPVTQSKFLHTVTAGYVCGAIFVLGISSYYILKGRDLGFAKRSF
                     SVGASFGLIAIISVLIMGDESGYEIGKAQPTKLAAMEGEFETQPAPAAWNAFVIPNTA
                     EMKNEFSIHIPYAAGIIATRSLDKEIKGLKDIRVINEQRVRTGMVAYGLLEKLRSGNY
                     TEADKEAFKAVQKDLGFGLLLKQYTPNVVDATEEQIQKAADNTIPNVGPTFWAFRVMM
                     LSGFLMLILITTAFIQNMRGKVESSRFLLRGLLFGLPLPWIAIESGWFLAEYGRQPWA
                     IYEMLPVGVSNSALTTGDLWFTIGLLCGLYTLFLVVEMYLMFKYGRLGPSALKTGRYY
                     FEQSTK"
BASE COUNT         1121 a          645 c          507 g          861 t
ORIGIN      
        1 tttttatcta tcaaaaataa aaatagagac ataaaaatgc ctctatttaa caagtaaaat
       61 ccataatgcc ggaataacaa taaggtaacc tagaagtggt aaaaaaatca ctttccaaat
      121 agcagctcgg atttcaaatc caattccatg tacccataaa atagccatac cataaaatac
      181 caacattgct aaatacggcg tttttccacc aaaagaaaga gcgaaagcgg tcgaatttac
      241 ccaaatagtt gcaaataata cagccgctaa aataaaagaa agggctttta acaagccctt
      301 tgctgttaag ttatagagtt tatgaatcat tactcgtcaa acttacctgt tttttcaatg
      361 ctgatcgtga tgactgttgc aaagaagatt gcaagcaata cacctaagac ccaagttaca
      421 taaaacatat tgtgtctcct tattagtaag ctgctttatt ttcttctaaa taactagcat
      481 ctaaacgacc atacattttg atatatgacc aaatagtata tgctaatact aacggtacaa
      541 agataccagc aacaaataac attacagtta atgttgtgtg gctagcagta gcgtcccaaa
      601 ccgttaagct catttctgga tgagtaattg acggcataat gaatgggaac attgatactg
      661 cagcagttaa aatcacccca acaatcatca atgatgatga gaagaatgct aaacctgaac
      721 gatttgcttt tgaggcaata atagtcagta atgcacctac tacaactaat gcaggaacaa
      781 cccataatac aggcatttca gcataatttc taaaccatgc accagcttga accgctacag
      841 ttttattcgt caatagagat tgtgcattat gatctaactc gcttgtgatc acaaaaccat
      901 ctttaaaata taaccataca cctgcaagaa taaatgccac taatacaata gaagctgtca
      961 cttgtgccac ttttcttgag ttgttatgta gctcatcagt agttttcatt tgtaaccaag
     1021 ttgcaccctg tgtggttaac atcattaagc tgataatacc gcacactaat gcaaatggat
     1081 ttaataaacc aaagaatgaa cccgtatatt gtgattggtt aatattatta aattcaaatg
     1141 gtacaccttg taataagtta ccgaatgcaa caccgaatac taatgatgga acgaaaccac
     1201 caataaataa tccccaatcc caagcactac gccatttcgg attatcaatt tttgcacgat
     1261 actcaaaacc tacagggcgg aaaaataatg ctgcaagcac aagcaccatt gcaatataga
     1321 aacccgagaa cgaagttgca tagactgttg gccatgcagc gaagatcgca ccaccagcgg
     1381 tcaataacca tacttggtta ccatcccagt gaggggcaat tgtgttgatc ataatacgac
     1441 gttcaacatt acttttacca atgataggta aaaggtttaa tacgcccata tcaaaaccat
     1501 cagtaattgc aaagccaatt aacaataccg caattaagat ccaccaaata aagcggagaa
     1561 tttcataatc gatcatttct aactcctatt atttagttga ttgttcaaaa taataacggc
     1621 ctgtttttaa tgcactaggg ccaagacgac catatttgaa cattaaatac atttctacaa
     1681 ctaagaatag tgtatataaa ccacacaata agccgattgt aaaccataaa tcacctgttg
     1741 ttaatgcaga gtttgacaca ccgacaggta acatttcata gatagcccaa ggctgacggc
     1801 catattcggc aaggaaccaa ccagattcaa tcgcaatcca aggtaacgga agaccgaata
     1861 acaagcctct taataagaaa cggctacttt ctactttacc acgcatattt tgaataaatg
     1921 ctgtcgtaat caagataagc atcaagaagc cagataacat cattacacgg aatgcccaga
     1981 atgtagggcc tacatttgga attgtattat ctgctgcttt ttgaatttgt tcttcagtcg
     2041 catcaaccac attaggtgta tattgtttta acaataaccc aaagcctaaa tctttttgta
     2101 cagctttaaa tgcttcttta tccgcctctg tataattgcc agaacgtaat ttttctaata
     2161 gtccgtaggc taccataccg gtacgaacac gttgttcatt aattacacga atatctttta
     2221 ggcctttaat ttctttatct aaagaacggg ttgcaataat accagctgca taaggaatat
     2281 gaattgagaa ttcatttttc atttccgcag tatttggaat cacaaaagca ttccaagcag
     2341 ctggagcagg ctgagtttcg aattcacctt ccattgcagc taatttagtc ggctgagctt
     2401 taccgatttc ataaccagat tcatcaccca taatcagtac agagatgatc gcaattaaac
     2461 caaaacttgc acctactgag aatgaacgtt ttgcaaagcc taagtcacga ccttttaaga
     2521 tatagtatga actaatacct aatacaaaaa tagcaccaca aacatagcct gcggttacag
     2581 tatgtaagaa tttagattgt gtaactgggt ttaatacaag ttcagagaaa cttgaaagct
     2641 ccattcgcat ggtttcaaag ttgaattcag aagcaatcgg gttttgcatc caaccattag
     2701 caactaaaat ccatagtgca gagaagttag aaccaaacgc aacacagtaa gttgctaata
     2761 aatgtttcgc tttcgataaa cgttcccagc caaagaagaa caagcccaca aaggtagatt
     2821 ctaagaagaa cgccattaaa ccttcaatcg ctaatggagc gccaaaaata tcacctacat
     2881 aatgagaata atatgaccag ttcataccaa actggaattc cattgtaata ccagttgtta
     2941 cccctagggc aaagttaata ccaaataatt taccccagaa tttagtcata tctttatata
     3001 cttctttacc cgtagttaca tacagggttt ccatcaccac aaggacgaaa gaaagaccta
     3061 atgtaagcgg tacgaataag aagtgataga gtgcagttag agcaaactgc aaacgtgaga
     3121 gttcaactac atct
//
LOCUS       DANLZC010000199         3116 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.199, whole genome shotgun sequence.
ACCESSION   DANLZC010000199 DANLZC010000000
VERSION     DANLZC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.199"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            108..2639
                     /locus_tag="PXE44_002434"
     CDS             108..2639
                     /locus_tag="PXE44_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5589892.1"
                     /translation="MQREYEKLGASISSVSRKRTEENRALKDSRHQQAKQLLYFRSLR
                     RELRASGINIKAFAQSETNLTDKIAKANQLIDQQKAKLEKLNAVKARNEKYWATVEKV
                     KNTSERLHNLGQRSMISGAAISAPVVGLGKGVVGMAQTAGKFEQFNAILEVTEGSAEK
                     AKQSFDWVKQFAVDTPSNLDEAMEAFVKLRAYGLDPTNGLLQTLGDTGAAMGKPVMQA
                     VEAIADALTGENERLKEFGIKGSVVKGTNIIEYAYTDKLGKQQVAKVNKNNRKEIEQT
                     LTRIFNEKYAGAMEKQAKTLVGIWAKIEDYWTNFQMQVMESGAFDWIKNKLQWVLDSL
                     DKMAENGELQKWAEDVGAVIQETVQGLWAFGEKIIEVVKWLASLARENKGLIATFVKW
                     SAILGSSLTVLGAFAVVASFALYPVARLALGIGKFTGASTLLNKALFDSNDKFRLFNK
                     SLYSSGTTAAFVKSKFNQLATLPNVLGSSFNKLGGGIKMLIPQLKTKVFWLNMLKGIL
                     GKLWSAIRFIFSPLKLLLGIFSPIGLAITAISVAGVALYRNWEKVRAFFGGFLQGLSK
                     GLAPVLERFKPLGKLFETISNWVKSTFKWLSDLLTPADKTSESLDKASSAGEKFGLAL
                     AGAIDLVTSPVQWLVDQFKWISENMPSWDTIGEGVKKAATAIVPTEHAKQITKTAEMS
                     NNMYDPNYTPPQLEQKWIGGLVGNGKGRGFATGGYTGNGGKYEPAGIVHKGEYVMTKE
                     ATARLGVANLNRLNYGKVAGLTALASSVAFAQPMPAVKIDSRPPLTASQPSPTVAPVS
                     QNIHITINATGGQDPQAIARLVAMELEKQQRQAQARARSSLRDRG"
     gene            2704..2880
                     /locus_tag="PXE44_002435"
     CDS             2704..2880
                     /locus_tag="PXE44_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589893.1"
                     /translation="MFKEKGYDEFVNECVRKGREEHKAGLGFTLEQSRLHIKKTIEQK
                     SQELNEFQREVVYG"
     gene            2873..>3116
                     /locus_tag="PXE44_002436"
     CDS             2873..>3116
                     /locus_tag="PXE44_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5589894.1"
                     /translation="MAKITIKPQALSQFDEIVKSVIEFTGFETSGIRLQSDIFDKIEQ
                     IAFMPLSGRAREDGTRETFVRRYRIVYDYDRQADEVI"
BASE COUNT         1071 a          509 c          722 g          814 t
ORIGIN      
        1 agctgaaagt gaaaatggat gcgaaaaaaa gggaatttca acaaattacg aaaggtggtt
       61 cagcttggag tgcaagagcc gcatttttag ataaagattt tgaaaaaatg caaagagaat
      121 atgaaaaact cggtgcatct atttcaagtg tcagccgaaa aagaacagaa gaaaatcggg
      181 cattaaagga ttctcgccat caacaagcta agcaactttt atattttcgt agtttaagac
      241 gtgaattgag agcaagcggc attaatatta aagcttttgc tcaaagcgaa accaacttaa
      301 cagacaagat tgcaaaagca aatcaattaa tcgaccaaca aaaagcaaaa ttagaaaaac
      361 taaatgcggt taaagctcga aatgaaaaat attgggcaac tgtcgaaaag gtaaaaaata
      421 ccagcgaacg tttgcataat ttaggacaac gctcaatgat tagtggtgcg gcaatttctg
      481 cccctgttgt tgggctaggg aaaggggttg tcggaatggc tcaaacagcg ggtaaatttg
      541 aacaatttaa tgctatttta gaggtaaccg agggaagtgc tgaaaaagcc aagcaaagtt
      601 ttgattgggt taaacaattt gcagtagata ccccctcaaa ccttgatgaa gcaatggaag
      661 cctttgtaaa gttaagagca tacggtcttg atcctaccaa tggcttacta caaacattag
      721 gtgatacagg tgctgcaatg ggtaagcctg taatgcaagc agttgaggca atagcagatg
      781 ctttaacggg tgagaatgaa cgcttaaaag aatttggtat taaaggaagt gtagttaaag
      841 gtacaaatat tattgagtat gcctacactg ataaattagg caaacaacaa gtcgcaaaag
      901 taaacaaaaa taaccgaaaa gaaattgagc aaaccctaac ccgtattttt aacgaaaaat
      961 atgctggagc aatggaaaaa caagctaaaa cgctagttgg tatttgggca aaaattgaag
     1021 attattggac aaatttccaa atgcaagtga tggaatcggg agcatttgac tggattaaaa
     1081 ataaattgca atgggtgtta gacagtttag ataaaatggc tgaaaatggc gagttgcaaa
     1141 aatgggctga agatgtcgga gccgttattc aagaaacagt acaaggctta tgggcttttg
     1201 gtgaaaaaat tattgaagtg gttaaatggt tagcgagctt ggcacgggag aataaagggc
     1261 taattgcaac attcgttaaa tggtctgcta ttcttggctc tagtttaacg gtgctaggag
     1321 cttttgcagt ggtggcaagt tttgcattat atcctgtagc aagattggct cttggtatcg
     1381 gcaaatttac aggtgcaagc accttattaa ataaggcatt atttgatagt aatgataaat
     1441 tccgattatt caataaatca ctttattcat caggcactac ggctgctttt gttaaatcaa
     1501 aatttaatca gttagcaact ttaccaaatg tgctgggaag tagttttaat aaattaggcg
     1561 gtggcatcaa aatgctaatt ccacagctta aaacaaaagt attttggctc aatatgctaa
     1621 aaggtatttt aggcaagttg tggagtgcaa tccgttttat tttctcaccg ttgaaattat
     1681 tacttggtat tttctcgccg attggcttag cgattacagc tatttcggta gcaggggtag
     1741 cgttatatag aaattgggag aaggtcagag ccttttttgg aggctttttg caaggattat
     1801 ctaaaggtct tgcaccagta ttagagagat ttaaaccttt aggaaaacta tttgaaacta
     1861 tatcaaactg ggttaaatct acgtttaagt ggttatccga tttattaacc ccagcggata
     1921 aaacatcgga aagtttagat aaagcgagtt ctgcaggaga aaaattcggg cttgcattgg
     1981 ctggtgcaat agacttggta acatcacctg ttcagtggct agtcgatcag tttaagtgga
     2041 tttctgaaaa tatgccgagt tgggacacta ttggtgaggg tgtaaaaaag gcagctactg
     2101 caattgttcc caccgagcac gccaagcaga ttactaaaac ggcagaaatg tcaaacaata
     2161 tgtatgaccc caattatact ccaccacaac ttgagcaaaa atggattggt ggcttggttg
     2221 gtaacggtaa aggaagaggt tttgccacag gcggctacac cggcaatggt ggcaaatatg
     2281 agcctgccgg tattgtgcat aagggcgagt atgtaatgac caaagaggca actgcacgct
     2341 tgggtgtggc aaatcttaac cggttaaatt atggcaaagt tgctgggcta actgcacttg
     2401 cgtcaagtgt tgcgtttgct caaccaatgc ctgcggtcaa aatcgacagc agaccaccgc
     2461 tgacggcaag ccaaccaagc ccaacagttg caccggtgtc gcaaaatatc catatcacca
     2521 tcaacgccac cggtgggcaa gacccacaag caatcgcccg actggttgca atggagttgg
     2581 aaaaacaaca acgccaagca caagcaagag caagatcgtc tttgagagac aggggctaat
     2641 ttgacaaagt agaatgaaaa gcggacaatg ctctaaattg tccgcttttt ataggagcaa
     2701 atgatgttta aagaaaaagg atatgatgaa tttgtgaatg aatgtgtgcg aaaaggtaga
     2761 gaagaacaca aagctggttt aggatttacg ctagagcaat ctcgcttaca tatcaaaaaa
     2821 accattgagc aaaaatcaca agagctaaac gaatttcagc gtgaagtggt ttatggctaa
     2881 aattaccatt aaaccacaag cattgagtca atttgacgag attgtaaaaa gtgttatcga
     2941 atttaccggt tttgaaacga gtggtattcg attacaaagt gatatttttg acaaaataga
     3001 gcaaattgca tttatgccac tctctggaag agcaagagaa gatgggacaa gagaaacttt
     3061 cgtcagacgt tatcgtattg tgtacgatta cgataggcag gcagatgaag taatta
//
LOCUS       DANLZC010000200         3077 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.200, whole genome shotgun sequence.
ACCESSION   DANLZC010000200 DANLZC010000000
VERSION     DANLZC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.200"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            29..667
                     /locus_tag="PXE44_002437"
     CDS             29..667
                     /locus_tag="PXE44_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL5589895.1"
                     /translation="MELKHTIEQRKTIKAFNSTIKISRTELEEMVALSQLAPSKANLQ
                     PWRFVIIDVEILKAALSKNVAFNGPPCETASAIVVILADLQYEKLLGAIVDQSVETGC
                     LHKEFRERKFDFLLNLHNQATQAEIRDQVLIDTSLAAMQLMLIAKDKGYDTHAIGVFE
                     RPAVLKLLNIDAERYLPVMLLAIGKAANPALPSCRLPLEYTVSWNDGQGFKI"
     gene            762..1286
                     /locus_tag="PXE44_002438"
     CDS             762..1286
                     /locus_tag="PXE44_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDL5589896.1"
                     /translation="MHSELNTRANILGKIIFASRWLQLPIYLGLIVVQGIYAYKFIKS
                     LYHLVINLNEMDSNTVMLAVLNLIDVVMIANLLVMVIVGGYETFVSRLKVDDHPDQPE
                     WLDHVNATILKVKLSMAIISISSIHLLQTFINASNLDEKQMMWQVVIHLAFIVSALAM
                     AYIDKMLNSGHMKH"
     gene            1363..2733
                     /gene="gorA"
                     /locus_tag="PXE44_002439"
     CDS             1363..2733
                     /gene="gorA"
                     /locus_tag="PXE44_002439"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL5589897.1"
                     /translation="MAKHYDYIAIGGGSGGIASINRAASYGQKCAIIEAKHLGGTCVN
                     VGCVPKKVMFYGAQVAEAINHYAPDYGFDITVNNFDYAKLVESRQAYIGRIHTSYGNV
                     LAKNNVDVLNGFARFKDAKTLEVSYADGSVEEVTADHILIATGGRPTVPNVKGAEYGI
                     TSDGVFALDALPKSVAIVGAGYIAVELAGVFNSLGVNTHLFVRQHAPMRNQDPLIVDT
                     LLEVLAQDGIELHTQATPQEVVKNADGSLTLKLQDGRETSVESLVWAIGREPATDKIN
                     LEAAGVETNERGFIKVDKFQNTNVPNIYAVGDIIEGGIELTPVAVAAGRRLSERLFNN
                     KPNEHLDYSLVPTVVFSHPPIGTVGLSEPKAIEQYGAENVKVYKSSFTAMYTAVTQHR
                     QPCRMKLVCVGEGEKVVGLHGIGFGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSE
                     EFVTMR"
     gene            2744..>3077
                     /locus_tag="PXE44_002440"
     CDS             2744..>3077
                     /locus_tag="PXE44_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589898.1"
                     /translation="MLRNFMIMLHFIYGFAMKNAFSFFVISLFLTMNSISYASNPIPS
                     NKEKLNGVKKQQDKPKFRISERKVLKNKEKDEEVSNFSMEELRVGAQRPLDDSVIQYF
                     KSQCRYAFM"
BASE COUNT          966 a          439 c          663 g         1009 t
ORIGIN      
        1 caattgtttg agagatagga gaaaagagat ggaattgaaa catacgattg aacagcgtaa
       61 aactatcaaa gcttttaatt caacaattaa gattagtaga actgaattag aagaaatggt
      121 cgctttatcc cagcttgctc cctcaaaagc aaatctgcaa ccttggcgtt ttgtcattat
      181 tgatgttgaa attcttaaag cagcattatc aaaaaatgtt gcctttaatg gcccaccttg
      241 tgaaacagcg tctgctattg tggtgatatt ggcagatttg caatatgaaa aattattagg
      301 cgcaattgtt gatcaatcgg tagaaactgg gtgtttgcat aaagagtttc gtgaacgtaa
      361 atttgatttt ttactaaatc ttcataacca agcaactcaa gcagaaatta gagatcaggt
      421 attaattgat actagtttgg cggcaatgca attaatgcta attgccaaag ataagggcta
      481 tgatactcat gcgataggcg tgtttgagcg ccctgctgta ttaaaattat tgaatataga
      541 tgcagaacgt tatttgcctg ttatgctact agctatcggt aaagcagcaa acccagcgtt
      601 gccaagttgc cgtttacctc tagaatatac ggtttcttgg aacgatggac aaggttttaa
      661 aatataaggt gcaagcggtt aaatatctta attttttggc aaaaaattgt taaaatttga
      721 gcgttgtaat ttatttttgt attgctattt ataggagata tatgcattcg gaattaaata
      781 ctagggcaaa tattttaggt aaaattattt tcgcaagtcg ttggttacaa ttacctattt
      841 atttagggtt gattgttgta caggggatat atgcgtataa gtttattaaa tcattgtatc
      901 atctagttat caatttaaat gaaatggatt caaatactgt tatgttagcg gtattaaatt
      961 taatagatgt ggtgatgatt gcaaatcttt tggttatggt tattgttgga ggatatgaaa
     1021 cttttgtctc tagattaaaa gtagatgatc accctgatca gccagaatgg ttggatcacg
     1081 ttaatgcgac tattttgaaa gtaaaacttt caatggcaat tatcagtatt tcttctattc
     1141 atttattaca aacatttatc aatgcttcaa atcttgatga aaagcaaatg atgtggcaag
     1201 tagttattca cttagccttt attgtttcag cattagcgat ggcgtatatt gataaaatgt
     1261 tgaattcagg gcatatgaaa cattagtctt ctatgcaaat ttaatttgat aaagcgctta
     1321 acgaataagt tgcttttgtt tttatagatc gaggaatagc ttatggctaa acattatgat
     1381 tatattgcta tcggcggtgg tagtggtggt attgcttcaa ttaatcgagc agcaagctat
     1441 gggcaaaaat gtgccattat tgaagcaaaa catttaggtg gcacttgtgt gaacgtggga
     1501 tgtgtaccca aaaaagtgat gttctatggt gcacaagttg cagaagcgat taaccattac
     1561 gcccctgatt atggttttga cattacggta aataattttg attatgccaa attagttgag
     1621 agtcgccagg cctatattgg gcgcattcat acctcatatg gtaatgtgct ggcaaaaaat
     1681 aatgttgatg ttttaaatgg ttttgctcgc tttaaagatg ctaaaacatt agaagtgagt
     1741 tatgctgatg gttccgttga ggaggtcaca gcagaccata ttctgattgc aacaggtggt
     1801 cgtccaactg tacccaatgt gaaaggggct gaatacggta tcacttctga tggtgtattt
     1861 gcactagatg ctttaccgaa gtccgtagca attgtggggg cagggtatat tgcagttgaa
     1921 ttagctggag tatttaatag tttaggagta aatacccatc tttttgttcg tcaacatgct
     1981 ccaatgcgta atcaagaccc attaatagtg gatacattgc ttgaagtatt agctcaagat
     2041 gggatcgaat tacatacaca agcgactcct caagaagtag ttaaaaatgc agatggttca
     2101 ttgacattaa aattgcaaga tggtcgtgaa acctctgttg aaagcttggt gtgggctatt
     2161 ggtcgtgagc cagcgacaga taagattaat ttagaggctg ctggtgttga aaccaacgag
     2221 cgtggtttta tcaaagtgga taaattccaa aatactaatg tgccaaatat ctatgctgtt
     2281 ggtgatatta ttgaaggtgg tattgaatta actcctgttg cagttgcagc tggtcgcagg
     2341 ctatcggaac ggttgttcaa taataaacca aatgaacatt tggattatag cttagtaccg
     2401 acagtcgttt ttagccaccc accaataggt actgttggtt taagtgagcc aaaagcgatt
     2461 gagcaatatg gggcagaaaa tgtgaaagtg tataaatctt catttactgc aatgtatact
     2521 gcggttacac aacatcgtca accttgccga atgaaattag tttgtgttgg ggaaggggaa
     2581 aaagtggttg ggttacatgg tattggtttt ggtgttgatg aaatgattca aggttttgct
     2641 gttgcaatta aaatgggcgc aacgaaagct gattttgaca acacagtggc tattcacccg
     2701 acaggatctg aagaatttgt aaccatgcgc taaatgtaat aaaatgttac gtaattttat
     2761 gattatgcta cattttattt atgggtttgc tatgaaaaac gcttttagct tttttgttat
     2821 ttctcttttt cttacgatga atagtattag ttatgcaagt aatcctattc ctagtaataa
     2881 agaaaaatta aacggagtaa aaaaacagca agacaagcct aagtttagaa tatctgaacg
     2941 taaggtattg aaaaataaag agaaagatga agaggttagt aatttttcta tggaggaatt
     3001 acgtgttgga gcacaacgac ctcttgatga ttctgtgatt caatatttta aatctcaatg
     3061 ccgatacgca tttatgt
//
LOCUS       DANLZC010000201         2993 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.201, whole genome shotgun sequence.
ACCESSION   DANLZC010000201 DANLZC010000000
VERSION     DANLZC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.201"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..593
                     /locus_tag="PXE44_002441"
     CDS             <1..593
                     /locus_tag="PXE44_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589899.1"
                     /translation="KWLMLITLIFGITACFSSLEFDESKQEKINSFLIVGNKLYVVGE
                     LHDYEFEHQQVAELNNFLKSLIANKIKSVEANFEVKESKKVEGSYRIILDGKSLTESE
                     RSTVMDTFHFNSAQNPSITYQAEGNLVKLANREELLQKFRFKQALEANVRYYKMKSGL
                     QNTAEGALSIVMLPVAVVAVLPFYMFWVGACATYGC"
     gene            604..1089
                     /locus_tag="PXE44_002442"
     CDS             604..1089
                     /locus_tag="PXE44_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDL5589900.1"
                     /translation="MYSLYLKILSECDWCCDPKTLSTYQQEWLLHEGSLTQKLYDVAT
                     NFNVEVVQEKWIAKNSEKTTACNEDVWSREVLLKDGEKNWIFAQTLVPRSTIENIAQN
                     VLELGNQPIGLWLFSQNPTRTKLEWTQTENGLFARRGVYEIRGYSLEIRELFLETFPY
                     L"
     gene            complement(1127..1996)
                     /locus_tag="PXE44_002443"
     CDS             complement(1127..1996)
                     /locus_tag="PXE44_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL5589901.1"
                     /translation="MKNINTQAVGWIGLGQMGEPMAKHLLNSGVNVGVYNRDPAKAEK
                     VVEAGGTLYPSVLELVKAYDVIFLMIADYAAVQQVLSDEVLAELNGKVVVNMSTISPS
                     QNQAVEALLAQHGAEFVEAPVSGSSKVAEAGKLLVLAAGKEETVELLKPLFAAFSTTT
                     FYYGEVGKAGGIKLMINSLLGIFIQAYGEALNFADQYGIPKEKVIEMISGSFMNSPIF
                     QAKVPMYQANEFPPAFMMKHMTKDFNLASEEIAKLGKSYPLIEQATKTYNQANDSGLS
                     EVDMAAIYQFLAK"
     gene            2126..2917
                     /locus_tag="PXE44_002444"
     CDS             2126..2917
                     /locus_tag="PXE44_002444"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL5589902.1"
                     /translation="MKPTILLYDSGMGGLTIYDEIRKVLPHAHYLYCFDNAFFPYSEK
                     SEAVLIERAVKIVQKIAEKQPLDMVVVACNTASTVVLPALREHFNFPIVGTVPAIKPA
                     AAISETKVIGLLATKGTVARPYVDELIRKYAQDCTVEKIGTTDLVEIVEEKQRSGKVD
                     MNRLQKVVEAWQGKPELDTVILGCTHFPLVKAELQQLLPDVKYFVDPGVGIAKRVLSL
                     LEERKFQQKADFENQAFCTKQSSAFNIKQDVMQNWGFNNLSVIDI"
BASE COUNT          914 a          556 c          620 g          903 t
ORIGIN      
        1 aaaaatggtt gatgttaatt acattaattt tcggcattac cgcttgtttt tccagtttag
       61 aatttgatga aagtaaacaa gaaaaaatca attcattctt gattgttggt aataaattat
      121 atgtggtggg tgaactgcac gattatgaat ttgagcatca gcaagttgcg gaactgaata
      181 attttctcaa aagcctaatt gctaataaaa tcaaatctgt tgaggcaaac ttcgaggtta
      241 aggaatccaa aaaagtggaa ggtagttata gaattatttt agatgggaaa tcacttacag
      301 aaagtgaaag aagtacggtg atggatacct ttcattttaa ttcagctcaa aatccatcta
      361 ttacttatca ggcggaaggg aacttggtga aattagccaa tagagaagag cttttgcaaa
      421 aattccgatt taaacaagcg cttgaggcga atgtgcgtta ttacaaaatg aaaagtggtc
      481 ttcaaaatac ggctgaagga gcgttaagta ttgtaatgtt gcctgttgct gtagtagctg
      541 ttttaccatt ctatatgttt tgggttggtg cttgtgccac ttacggatgt taaataactt
      601 tttatgtatt ctctatacct gaaaatttta tcggaatgcg attggtgttg tgatccaaaa
      661 acactttcta cctaccagca agaatggctt ctgcacgaag ggtcacttac ccaaaaattg
      721 tatgatgtcg caaccaattt caacgtggaa gtagtacaag aaaagtggat tgcaaaaaac
      781 agtgaaaaaa cgaccgcttg taatgaagat gtttggtcgc gagaagtgtt actgaaagat
      841 ggtgaaaaaa attggatttt tgcccaaact cttgtgccaa gatcaacaat cgaaaatatc
      901 gctcaaaacg tgttggaatt gggcaatcag ccgattggtt tatggctatt ttctcaaaat
      961 cctactcgca ccaaattaga atggacacaa acagagaacg gtttattcgc tcggcgtggg
     1021 gtgtatgaaa ttagagggta ttctctggaa attcgagagc tttttttaga aacctttcct
     1081 tatttataaa aaaatcggtt ggtaatgaca ccaaccgatt ttcatcttat tttgccaaga
     1141 actgataaat tgcagccata tccacttcgc tcaagcctga atcattggct tgattgtagg
     1201 ttttagtggc ttgctcaata agcgggtaag atttgcctaa tttcgcaatt tcttcgcttg
     1261 ccaaattaaa atctttggtc atatgtttca tcataaacgc aggcggaaat tcattagctt
     1321 ggtacatcgg cacttttgct tggaagatcg ggctgttcat aaacgagcct gaaatcattt
     1381 cgattacttt ctctttcgga atgccatatt ggtcggcgaa gtttaacgcc tcgccatagg
     1441 cttgaatgaa gatgcccagt agcgagttga tcatcaattt aatcccaccg gctttaccca
     1501 cttcaccata atagaaagtg gtggtactaa aggcagcgaa aagcggtttg agcaactcaa
     1561 cggtttcttc tttaccggca gccagtacca atagtttacc tgcttctgcc actttgcttg
     1621 aaccggaaac cggagcttca acaaattctg caccgtgttg ggctagtaga gcttctaccg
     1681 cctggttttg gcttggggaa atggtgctca tattcaccac tactttgccg tttaattcag
     1741 ccaacacttc atcgcttaac acctgttgca cggcggcata gtctgcaatc atcaagaaaa
     1801 tcacatcgta ggctttcacc aattccaaca cagaaggata taatgtgccg cctgcttcaa
     1861 ctaccttttc tgcttttgct gggtcgcggt tgtaaacgcc cacgttcact ccgctattta
     1921 gcaaatgttt tgccatcggt tcgcccattt gccctaaacc aatccaaccg actgcttgtg
     1981 tattaatgtt tttcattatt tttctcctta ctgaataaag caaattgatt attgcgattt
     2041 tccctcatca agtcaaatag agagaacttg aatatagcaa tggaaaagag tagcatttta
     2101 gcacttactt ttttgaacaa taccaatgaa accaacaatt cttttatacg attccggaat
     2161 gggcggattg acgatttacg atgaaatccg aaaagtgtta ccgcacgctc actatttata
     2221 ttgctttgat aacgcttttt tcccatattc cgagaagtcg gaagcagttt taattgagcg
     2281 agcggtcaaa attgtgcaaa aaattgcaga aaaacaaccg cttgatatgg ttgttgttgc
     2341 ttgtaatacg gcaagcacag tggttctgcc ggcattgcgt gagcatttta attttccgat
     2401 agtaggaact gtaccggcaa ttaaaccggc agcggcaatt tccgaaacta aagtcatcgg
     2461 cttgctcgcc actaaaggca cggttgctcg cccttatgtt gatgaattga taagaaaata
     2521 tgctcaggat tgtacggtag aaaaaattgg cacaaccgat ttggttgaaa ttgttgaaga
     2581 aaaacaacga agcggtaaag tggatatgaa ccgactacaa aaagtggtcg aggcttggca
     2641 aggtaagccg gaattggata cggttatact cggttgtacc cattttccct tagtcaaagc
     2701 tgaattacag caattattgc ccgatgtgaa atattttgtt gatccgggtg taggtattgc
     2761 caaacgggtg ctaagccttt tggaagaacg caaatttcaa caaaaagccg attttgaaaa
     2821 tcaagcattc tgcactaaac aaagttctgc atttaatatc aaacaagacg ttatgcagaa
     2881 ttggggattt aataatcttt cggtgattga tatttaaaca aaacaatctg ttttgatgaa
     2941 aaaaggttca gttgagcaaa aactttaaaa aagggcttgc actcaatttt aaa
//
LOCUS       DANLZC010000202         2977 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.202, whole genome shotgun sequence.
ACCESSION   DANLZC010000202 DANLZC010000000
VERSION     DANLZC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.202"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            11..2101
                     /locus_tag="PXE44_002445"
     CDS             11..2101
                     /locus_tag="PXE44_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDL5589903.1"
                     /translation="MLSYLVFFSKIIKIPNLKGFLPHCEIRRRPLVGKTFIAQKRPNQ
                     IIGWGLRPSAVGSRAYAQKHQLPYIALEDGFLRSLGLGVKGYPPFSLVYDDIGIYYDT
                     TCPSRLEKLLLEKPLQDQEQIKLAERALSQILTYGLSKYNHALDFSEIPSAKEKIVLV
                     IDQTFGDMAVKYGQASEVDFNKMLETAISENPEAQIWVKTHPDVLSGKKKGYLTDLPQ
                     YTDRVRIITEDVNPASLIKLCEKVYCVTSQMGFEALLLGKKVVTFGVPWFAGWGVTDD
                     RNEAVQLLVQQNRRKDRSLLQLFYAAYFDYSRYINPNTGEKGSIFDVIDYLYKAKRLN
                     QRLSGNLYCIGMSLWKQAVIKPFFNLPQCRLHFVRSLNSLKKRPLADNAKILVWSNGK
                     ADVLQFAQQNNIPLLRMEDGFIRSVGLGANLVAPLSLVVDDLGIYFNAENTSRLEQIL
                     LSAEHFSETDLSYAETLRQKLVASHIGKYNVGGGDVKFNAQGKKIILVPGQVEDDASI
                     KSGSPQIKRNLDLLKKVRELNPSAYIIYKPHPDVVSGNRVGQIPTAETRRFADQMVDN
                     ANILDCILIADEVHTMTSLAGFEALLRGKKVHCYGLPFYAGWGLTEDRLKSARRGKSL
                     TLSQLIAATLIYYPLYVDPISRQFIDANQAIHILSQQKRNLTNQRIKRSWVSKQYGKL
                     QHLYRAFKYASHTK"
     gene            2124..>2977
                     /locus_tag="PXE44_002446"
     CDS             2124..>2977
                     /locus_tag="PXE44_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide modification protein"
                     /protein_id="HDL5589904.1"
                     /translation="MEHYLDELIINSQRILLLQGPIGSFFTEFARWLESEQKEVFKLN
                     FNGGDEYFYPNNSPNTFPYYDSLDNFEAYLIQFCQTHQIDNILCFGDNRYYHKVAKNV
                     CLKLKLVFWAFEEGYFRPEYITLEKWGVNAFSPIPKNHTFFLPYAELPVVSEPEKVSK
                     GFRCMAGKAIPYYWNAYKKRKAYAKYQHHRFLNLKYYLNLWTRSGIKRICYWLHDKGF
                     AKRVEKGEFGEFFIVPLQVYDDTQVRIHSEQKSVERFLREVLNSFAKFAPAHLNLIIK
                     HHPMDRGF"
BASE COUNT          948 a          477 c          621 g          931 t
ORIGIN      
        1 ttaatttttt atgcttagct atttagtgtt ttttagtaaa attataaaaa tccctaattt
       61 aaaaggtttt ttacctcatt gtgaaattag gcgaagacct cttgtgggaa aaacctttat
      121 tgctcaaaaa agaccgaacc aaatcatcgg ctgggggctt cgtccttcag cggtgggatc
      181 aagagcttat gcacaaaaac atcaacttcc ctatattgca ttagaagacg gctttttacg
      241 ctcactaggg ctgggtgtaa aaggctatcc gccattttct ttagtttatg atgatatagg
      301 tatttattac gataccacct gtccttctcg tttagaaaag ttattgcttg aaaaaccttt
      361 acaggatcaa gagcaaatca agcttgcaga gagagcatta tctcaaatct taacttacgg
      421 actttctaaa tataatcacg cactagattt tagtgaaatc ccctctgcca aagaaaaaat
      481 cgtgttagtt attgaccaaa cctttggtga tatggcggtg aaatatggtc aagcgagtga
      541 agtggatttc aataaaatgt tggaaacggc aatcagtgag aatccagagg ctcaaatttg
      601 ggtaaaaact caccccgatg tattaagtgg caagaagaaa ggctatttga ccgatttacc
      661 tcaatatact gatagggtca gaattattac tgaagatgtg aatccggcaa gtttgattaa
      721 attgtgtgag aaagtctatt gcgtaacttc gcaaatgggg tttgaggcat tattattagg
      781 taaaaaagtg gttacctttg gtgtaccgtg gtttgccggt tggggggtaa cagatgatcg
      841 taacgaagcg gttcaactat tagttcaaca aaatagacgg aaagatcgtt cattactgca
      901 acttttttat gctgcgtatt ttgattacag ccgttatatt aatccgaata ccggagagaa
      961 aggctctatt tttgatgtca tcgattatct atataaagcc aaaagattaa atcagcgctt
     1021 aagtgggaat ctatactgta ttggtatgtc gctatggaaa caggcggtaa ttaagccgtt
     1081 ttttaatttg ccacaatgtc gattacattt tgttcgttcg ctaaattctt taaaaaaacg
     1141 accgcttgca gataatgcaa aaattttagt ttggagcaat ggcaaagcgg atgtcttgca
     1201 atttgcacag cagaataata ttccattgtt aagaatggaa gatggattca ttcgctctgt
     1261 cggactaggc gctaatttag ttgcaccgct ttctttagta gtcgatgatt taggcattta
     1321 ttttaatgca gagaacactt cacgattaga acaaatttta ttgagtgcag agcatttttc
     1381 tgaaactgat ttgtcttatg ctgaaacatt aaggcagaaa ttggtggcta gccatattgg
     1441 aaaatataat gtgggcggtg gcgatgtaaa atttaatgct caaggtaaaa aaataattct
     1501 tgtaccgggg caggtagaag atgacgcttc gatcaaatca ggttctccac aaattaaaag
     1561 aaatctggat ttactgaaaa aagtgaggga attaaaccct agtgcctata ttatttataa
     1621 gcctcaccct gatgtggtaa gtggtaatcg agtcggtcag attcctacgg cagaaacacg
     1681 caggtttgcg gatcagatgg tggataatgc caatatttta gattgtattt taattgcaga
     1741 tgaagtgcat acaatgacat ctttagccgg tttcgaagca ttattaagag gtaaaaaagt
     1801 gcattgctac ggattacctt tttatgctgg ttggggattg acggaagata gattgaagtc
     1861 ggcacgcaga ggtaaatctt tgacattatc tcagttaatt gccgcaacat tgatatatta
     1921 ccctctgtat gttgatccta taagcaggca gtttattgat gccaatcaag cgattcatat
     1981 attgtcacaa cagaaacgaa acctaaccaa tcaaaggatt aaacgttctt gggtgagtaa
     2041 acaatatgga aaattacagc atctttatcg tgcgtttaaa tatgcttccc acactaaata
     2101 agcgtagagg gtaggaaatt aagatggaac attatttaga tgagctaatc atcaatagcc
     2161 agagaatttt attgttacaa ggcccgatag gctctttttt cactgagttt gcccgttggt
     2221 tagaaagtga gcaaaaagag gtcttcaagt taaattttaa tggcggagat gaatatttct
     2281 atccaaataa tagcccgaat acttttcctt actatgatag cttagataat tttgaagcct
     2341 atttaattca gttttgccaa actcatcaaa tagataatat cttatgtttt ggcgataatc
     2401 gttattatca taaagtggct aaaaacgttt gcttgaagtt aaagttggtc ttttgggcgt
     2461 ttgaagaagg gtactttagg ccagaatata ttacgttgga aaaatggggt gtaaatgctt
     2521 tttctccaat ccctaaaaat cacactttct ttttgcctta tgccgagtta ccagtagtca
     2581 gtgaaccaga aaaagtatct aaaggcttta gatgtatggc gggaaaagca atcccgtact
     2641 attggaatgc ctataaaaaa cgtaaagcat acgcaaaata tcagcatcat cgttttctga
     2701 atctcaaata ctaccttaat ttatggacaa ggtcgggaat taagcgaata tgttattggt
     2761 tacatgataa aggctttgcc aagcgggtag aaaaaggaga gtttggtgaa tttttcattg
     2821 tgcctctaca agtttatgat gacactcaag tgaggattca tagtgaacaa aaaagtgtag
     2881 agcgtttttt aagggaagtt ctgaattcct ttgccaaatt tgcaccggca catctcaatt
     2941 taattattaa gcatcacccg atggatagag gatttat
//
LOCUS       DANLZC010000203         2940 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.203, whole genome shotgun sequence.
ACCESSION   DANLZC010000203 DANLZC010000000
VERSION     DANLZC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.203"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..466
                     /locus_tag="PXE44_002447"
     CDS             <1..466
                     /locus_tag="PXE44_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589905.1"
                     /translation="KGDRNYQLYIDLATGELVSKLGKGLTTRAFDAAVKAFENAFNAK
                     DTAENTAPVESKETEQENKQETTKLVITKPTLKGSEKQIDSANEIINLFIAKIGDTSI
                     PVTATQEQITQAQAMVDNFFAMTNAGKWIEKFKGLNDNSEPKQFWLSVKYGW"
     gene            536..1054
                     /locus_tag="PXE44_002448"
     CDS             536..1054
                     /locus_tag="PXE44_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589906.1"
                     /translation="MTTQVTPQIMRIIGQIVAATYGDDVPTNVQTIILRYPIRGIGFI
                     SSRRELSINNGEIARLMDKIPGDLEDPKDGMPFDCQGAFWLGYYQYCKLSNDVKNYTS
                     KELSIIGESLYGTQWQSNLARDLRLSDARRVREWVAGERKIPFGVWADLTELVKAKKA
                     NLSSILKKLTID"
     gene            complement(1201..1440)
                     /locus_tag="PXE44_002449"
                     /pseudo
     CDS             complement(1201..1440)
                     /locus_tag="PXE44_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1601..1894)
                     /locus_tag="PXE44_002450"
     CDS             complement(1601..1894)
                     /locus_tag="PXE44_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589907.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYKHLKETGQIVV"
     gene            complement(2009..2608)
                     /locus_tag="PXE44_002451"
     CDS             complement(2009..2608)
                     /locus_tag="PXE44_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589908.1"
                     /translation="MSYGLSTFNINENFRSLSLQRKGRANFSNKIATIQAASDEIIVI
                     KYSDGQVCLLYRENGIATLATENATFAEYLIFSTNVPKSGGYGIEVYNSAGNVVFSSN
                     HKFLRPIEYVDLNPSKNVFRFTGQANRQYGIILSNFGFDFVINYDNGYSTSRSAKVSG
                     NFIEFGFCKYHYSGLYKTGMSYNSKAMYFNALVVDITGY"
     gene            complement(2609..>2940)
                     /locus_tag="PXE44_002452"
     CDS             complement(2609..>2940)
                     /locus_tag="PXE44_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589909.1"
                     /translation="IQTFDTANVSTFSTNERLFKYIGTKNIKYGKFSFTVTEKYEGQL
                     VFIISMLHSVHRKQATGYKTNIPNLIHLENVSINDKTISGEVVCIFPFSLPADDSDYI
                     QIVYGAY"
BASE COUNT          958 a          539 c          534 g          909 t
ORIGIN      
        1 caaaggtgat cggaattacc agttgtacat tgacctagcc acaggcgagt tagtgagcaa
       61 acttggcaaa ggattaacaa ccagagcgtt tgatgctgcc gtaaaagctt ttgaaaacgc
      121 ttttaatgct aaagacaccg ctgaaaatac agcgccggta gaaagcaaag aaacagagca
      181 agagaacaaa caagaaacaa ctaagcttgt aatcacaaaa ccaacattaa aaggctctga
      241 aaaacaaatt gattcggcaa acgaaatcat caatcttttt attgcgaaga ttggtgatac
      301 ctctatccca gtaacagcaa cgcaagaaca aattactcaa gctcaagcaa tggtagataa
      361 tttttttgca atgacaaacg ctggaaagtg gatcgaaaaa tttaaaggtc taaatgataa
      421 tagcgaacca aagcaatttt ggttatccgt aaaatatgga tggtagattg agataacaaa
      481 taagccgtaa gagcaaaggc ttacggctaa aacaaacaga taactggaga ttattatgac
      541 tacacaagta acaccacaaa ttatgcgcat tatcgggcaa attgtcgctg ctacttatgg
      601 tgacgatgtg ccaacaaacg tacagacaat aatcttgaga taccctataa gaggcatagg
      661 gtttatctct agtcgcagag agctatctat taacaatgga gagatagcaa gattaatgga
      721 taaaatacca ggcgacctcg aggaccctaa agacgggatg ccttttgatt gtcagggggc
      781 gttttggctt ggatactatc aatactgcaa attaagtaac gatgtaaaaa attacacatc
      841 aaaagagcta tccatcattg gcgagtcatt gtatggcact caatggcagt ctaatctagc
      901 gagagatttg agattatctg atgctcgccg tgtccgcgaa tgggtggctg gtgaacgtaa
      961 aatccctttc ggggtttggg ctgatttgac cgaattggtt aaagccaaaa aagctaactt
     1021 atcatcaatt cttaaaaaat taacaataga ttagcactga ctttaaagcc cctctcgggg
     1081 cttaattata gaaaataaaa tcacaatggc taattttgcc aaatttgcct gttaaaacgt
     1141 gcgaaaaatt acaaaaaacg gctagagcta tagccgtttt gagtgcagat gttaacagag
     1201 ttaagctcta gcacgtcctg ggccttgata ctgcacacca tcagttaaat gatcttgatt
     1261 ttcggttgct gctacagcag attctgatct tacatttaaa ctttgtgact gttgtaatgc
     1321 atcaataagt ttatttacca ctgttccgag tgttttaatt tgctcggttt gctcattaat
     1381 agtctgcttt aactcttgca gttttaatga ttgctcatcc acaaccacta atcgacggtt
     1441 agctcaattt gaggctctgt gctgccctct actatggcgt gtaaatccag cctaataccc
     1501 tctaaattta caggctcgcc atttgtttgc ctaaaaaaaa ggcgataact tacgtcatcg
     1561 cctctgaaaa acttaaattt atgcttttgc atttagaccc ctacactaca atctgccccg
     1621 tctcttttaa gtgcttatag atacgaccta acataatctg cgttggcgtt ttgccaatat
     1681 cctcttttgt tagcggtgca tctgtaatcg ctttggctga ttgagcatca atatatttgt
     1741 actcacttgt gatgattaca aagtttgtta cctcgccatc ggtatcatca cctgtgccaa
     1801 caacatactt agcattgagt gagccgtcat cttgagttga gtaacttgcg atagttgagt
     1861 acattgggtt taagattttg ttaaaagttg tcataatttt tccttttgtg gattgttgat
     1921 aaaaaaagac cgcttgtatt gctacaagcg gcgatgaatg tgtaatcaga attttagttc
     1981 gcctctatta aatgaaggcg tttaatgttt aatagcctgt tatatctaca accagagcat
     2041 taaaatacat tgcttttgaa ttataactca ttcccgtctt atacaaacca gaataatggt
     2101 atttacagaa cccaaattca ataaaattac cacttacttt ggcagatcta gatgttgaat
     2161 atccgttatc gtagttaatg acaaaatcaa agccaaaatt ggataagata atgccatatt
     2221 gcctatttgc ttgccctgta aatctaaaaa cattttttga tggatttaga tcgacatatt
     2281 caataggtct taaaaatttg tggtttgatg aaaatacaac atttcctgct gagttataaa
     2341 cctctatgcc gtaacctccg cttttcggta catttgtact aaaaatgaga tattcagcaa
     2401 atgttgcgtt ttctgtagct aatgtggcaa taccattttc tcgatatagc aaacatactt
     2461 gcccatcaga atatttaatg acaataattt catctgacgc tgcctgaata gttgcaattt
     2521 tattagaaaa gttagctcgt ccttttcttt gtaaggataa acttctgaaa ttttcattga
     2581 tgttaaaagt agatagtccg taactcattt aatatgctcc atatacaatc tgaatataat
     2641 ctgaatcatc agccggtaga ctaaaaggaa aaatacaaac aacttctcct gaaatcgttt
     2701 tatcgttgat cgaaacattt tctaaatgaa taagattggg aatattcgtt ttataacctg
     2761 ttgcttgttt tcggtgaact gaatgtaaca tactaatgat aaatacaagt tgtccttcat
     2821 acttttctgt taccgtgaat gaaaatttac catacttaat atttttagtt ccaatgtatt
     2881 taaataatcg ctcattggta ctaaatgtag agacattagc ggtatcaaaa gtttgaatac
//
LOCUS       DANLZC010000204         2910 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.204, whole genome shotgun sequence.
ACCESSION   DANLZC010000204 DANLZC010000000
VERSION     DANLZC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.204"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            177..1331
                     /locus_tag="PXE44_002453"
     CDS             177..1331
                     /locus_tag="PXE44_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HDL5589910.1"
                     /translation="MNQFFPLIFSLTLFSGVTLALEKAPRTMPTEGINKPLLGQILFF
                     DNMLSHNGSQNCSSCHNPDYAFIDNRTTPAKGVVSQGDTPTKFGKRNSPTMLYAKYSP
                     EFHFDEQSQEYIGGQFWDGRAKNLAEQAGMPPIDPNEMGMPNKLEVAKRLYNVPMYQR
                     FLGQFYGEKVWESYESVYAAMEDAIATFQQENKLLAPFDSKYDLFLKGEVQLTALEEK
                     GRALFFNKAQTNCSSCHQLQSAANHQEETFSNYHYYNVGVPGNPDLIAVNGFAPDFVD
                     LGLFENPNVKGDETQKGKFKTPTLRNVAVTALYMHNGVFKELKTVLLFLDSYNNPERK
                     INPETGTAWQQAEFEPTIAHQKLKAKPLSDEEIDALEAFLKTLTDERYRK"
     gene            complement(1379..2029)
                     /locus_tag="PXE44_002454"
                     /pseudo
     CDS             complement(1379..2029)
                     /locus_tag="PXE44_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250453.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            complement(2061..>2910)
                     /locus_tag="PXE44_002455"
     CDS             complement(2061..>2910)
                     /locus_tag="PXE44_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL5589911.1"
                     /translation="IIKPRCVITNIQQQTIVDMLDNRNKTTVTRYLSKRTDRDLVRYV
                     AMDMWRPYRQAVETMIPDATVIIDKFHVVRMANESLERARKAIRSALTPQQRRGLMRD
                     RFVLLKRRHELTDAEYMRFSGWTLNYPEIGQAYELKEAFFEIWDCQTRHQAQEAYYSW
                     LRQITPEMKAHYDPLIKAMGNWHDDIFAYFDHPITNAYTESLNNLIRVVNRVGRGYSF
                     EALRAKILFTEGFQKIKKPRYQRQRIPEGAMGRMPFYGVAEAGPSTNYGADISTLVRE
                     IEAGRL"
BASE COUNT          826 a          673 c          605 g          806 t
ORIGIN      
        1 tacgaggaat ctaaccgctt gttataacgc aaaacacctt gacttaaatc aagccactac
       61 ctcattttga ggggagattt atacgcagtg aaattttaaa ataagctaag gtttacggat
      121 aatcgttcaa cactattgaa ttatcactgt agttatcgtt aaaaaggtat gcttttatga
      181 accaattctt ccctctaatc tttagcttaa ctctattttc cggcgtaact ttggcactag
      241 aaaaagcacc tcgaactatg ccgacagaag gaataaataa gcccctactt ggacaaattc
      301 tattctttga caatatgctg tcacacaacg gttcacaaaa ctgctccagc tgccataatc
      361 cggattatgc ctttattgat aaccgcacta ctccggcaaa aggggtggtt tcacaaggag
      421 atactccaac caaattcggc aaacgcaact ccccgaccat gctttatgcg aaatactcgc
      481 ctgagttcca ctttgatgag caaagtcaag aatatatcgg cgggcagttc tgggacggac
      541 gagccaaaaa tctcgccgag caagccggta tgccgcctat cgacccaaat gaaatgggaa
      601 tgcctaacaa attagaagta gccaaaagat tatataatgt gccgatgtat cagcgatttt
      661 tagggcaatt ttacggtgaa aaggtatggg aaagttatga aagcgtttat gcggcaatgg
      721 aagatgcgat tgcgacattc caacaagaaa acaaacttct cgctccattt gattcaaaat
      781 atgatctatt tttaaaaggc gaggtacaat taactgcatt ggaagaaaaa ggcagagctt
      841 tatttttcaa taaagctcaa actaattgta gtagttgcca ccaattacag agtgctgcaa
      901 accaccaaga agaaaccttt agcaactatc actattacaa tgtaggcgta cccggtaacc
      961 cagacttgat agcggttaac ggcttcgcac cagacttcgt cgatttaggc ttatttgaaa
     1021 acccaaatgt taaaggtgat gaaacacaaa aaggcaaatt caaaacccca acattgcgta
     1081 atgttgccgt gactgcactt tatatgcaca acggtgtgtt taaagaatta aagactgttt
     1141 tgctgttttt agacagctac aataacccgg agcgaaaaat caaccctgaa accggcacgg
     1201 cgtggcaaca agcagaattt gaacccacta tcgcccacca aaaactcaaa gccaaaccct
     1261 taagcgatga agaaattgat gccctcgaag catttttaaa aaccttgacg gatgagcggt
     1321 atagaaaata aggctacaag cggtaaaatt ttggggaaat tttaccgctt gcgtttaact
     1381 accgtgttag ctcactttct aacttttcca atgcctgcaa atcttcccaa tctgcctctt
     1441 tctgttttaa acatttacgc tgaatatcaa atttttcgta ttgctctctg acaaagcttt
     1501 ccatttgttt atgcgtgatt gttccaccat ttcctaatac agaaaagccg ttaaactcaa
     1561 tcaacatatc tacatttctt cgccagaaac ctaacgtaag atcttggttg ttcttcacac
     1621 gtaattcggc actttcaaga aagatcatta ccaagcgatt taaagaatcc agctcattat
     1681 tatctaaata gtttttcgca atcacaatat cgcctttacg cacgactttt cctttccagc
     1741 tagttaatcc catattcatc ttagtcgcat cagcacgttg acaaatcaac tcagcggcag
     1801 tgtgtttggt tattgcataa atgagcttat tttgcgtttc tgcaaaaaac atttgcgtat
     1861 ttttatctgt tttgtcgtaa tcgctagaga gtttgaaaag ctctcgtact ttttgataga
     1921 aacgcaattc acttgcccga atctcacgaa tctgttcaag taattcatcg aaataatcta
     1981 aacgaccctg cggatttttc aaacgctcgc tatcaattaa aaaacctttt gggtatccgg
     2041 attacagggt tgaaacaggg ctataaacgc cccgcctcaa tctcccgcac cagtgttgat
     2101 atgtcagcgc cgtagttagt ggatggcccc gcttcggcaa cgccatagaa cggcatcctg
     2161 cccatagcgc cctcgggtat ccgttgacgc tgatagcgtg gcttcttgat cttctggaag
     2221 ccctcagtaa acaggatctt ggctcgcagc gcttcaaagc tgtagccacg gccaacacga
     2281 ttaacaacac gaatcaggtt gttcagtgac tcggtgtagg cgttggtaat ggggtggtcg
     2341 aagtaagcga aaatgtcatc gtgccagttg cccatcgctt tgataagcgg gtcatagtgg
     2401 gctttcattt ccggcgtaat ctgtcgcagc caagagtaat atgcctcctg tgcctggtgc
     2461 cgggtctgac agtcccaaat ctcgaaaaag gcttctttca gttcgtaggc ttggccaatc
     2521 tcagggtaat tcaatgtcca gcctgaaaaa cgcatgtatt cggcatctgt cagctcgtga
     2581 cggcgtttca gtaggacaaa cctatctcgc atcaacccac ggcgctgctg tggcgtcagg
     2641 gcgcttctga tggcttttct ggcgcgttcc agtgattcgt tcgccatcct gacaacgtgg
     2701 aacttgtcga tgatcaccgt ggcgtcagga atcatggttt ctactgcttg ccggtacggc
     2761 ctccacatat ccattgccac gtagcgcacc aggtcgcggt cagtgcgttt gctcaggtag
     2821 cgggtgacgg tggttttatt gcggttatcc agcatgtcca caatggtctg ctgctgaatg
     2881 ttggtgatca cgcagcgggg tttgatgatg
//
LOCUS       DANLZC010000205         2897 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.205, whole genome shotgun sequence.
ACCESSION   DANLZC010000205 DANLZC010000000
VERSION     DANLZC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.205"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..181
                     /locus_tag="PXE44_002456"
     CDS             <1..181
                     /locus_tag="PXE44_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5589912.1"
                     /translation="FRPRRVFERSAEKKKIHNLISRAHYDMATNEDSASEHLELFGED
                     IAEDSFDYPKPEKLI"
     gene            188..259
                     /locus_tag="PXE44_002457"
                     /pseudo
     CDS             188..259
                     /locus_tag="PXE44_002457"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            269..787
                     /locus_tag="PXE44_002458"
     CDS             269..787
                     /locus_tag="PXE44_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5589913.1"
                     /translation="MNRRYIGIEIGEHAKTHVVPRLKKVIEGEQGGISKAVNWQGGGS
                     FRFCELGEEVFDAFGSLNPNIRFDDLAAHIWYLENHFPLQKNSEKSPLVGTFNGKAYY
                     LLYNGILGDKRPQSGNVLTRKLMAELPYFAEFIQQGMDIVVYGEACRLGEVQLAEKKI
                     TFKQIPYDVMSR"
     gene            803..1792
                     /locus_tag="PXE44_002459"
     CDS             803..1792
                     /locus_tag="PXE44_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5589914.1"
                     /translation="MKLKNYQNEALNRVSHYFSACLQSDAKTAFSQIQPEYSYKIPSE
                     HSNLRDVPYVCVRIPTGGGKTLLASHSIARIAKQYLDCDFPVTLWPVPSRTIKTQTTE
                     ALKNPQHPYRAVLDKTFNREVMVIESEDFDLLRPQDFGNKAIVIVSTIQNFRVENQDG
                     RKIYAFNEKLTAHFERLLPHIQANLEKISTTDLQENGLTSKQTGKIKCSFANLLRAYQ
                     PLVIVDEAHNARTSLSFDVLSQLSPSAILEFTATPNTDRKAGSNVLYHVSASHLKAEE
                     MIKLPIVLTEHHNWQQAIDEAVINRGALAQKAQYESEYVRPIYCFKLSRKTAK"
     gene            1759..>2897
                     /locus_tag="PXE44_002460"
     CDS             1759..>2897
                     /locus_tag="PXE44_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589915.1"
                     /translation="MFQAEPKNGKVTVEVLKQYLIDELKIDEEEIAIVIGNQHELDNI
                     DLFNPQCKINYIITVEALKEGWDCPFAYIFCSVQNVSSSKEAEQLLGRVLRMPYAKHR
                     QIEDLNRAYAPLSSKSFGETARNLQDKLIGMGFEALEVAEMLRIPQQENWIGNEGDLF
                     YTPTTVLALTQLPEMSDLSEAKRSQLKITEQEGHILVQVKGNLSEKLEKALIRTATGK
                     KKEQIEQQLQIHQNRVEILSSPAEKGEIFKVIPQLCMNIQGEFDLADSQVLLDYQSWN
                     LLDYPAKLDGFRLQENSQSFEIDVEDKRVSYHFNQQQLSFSDDWLRNPVKRGFSLPLA
                     HQNFYPDFIVELIDGRILIVEYKGEQLKSNEDSKEKALIGNLWEK"
BASE COUNT          975 a          487 c          588 g          847 t
ORIGIN      
        1 ctttcgacca aggagagtat ttgagcgaag tgcggaaaag aaaaaaattc ataacttaat
       61 tagcagagct cattatgata tggcaacaaa tgaagactct gctagtgaac atctagaatt
      121 atttggggag gatattgcag aagattcatt tgattatcct aaacctgaaa aattaatcta
      181 atttgtatta gaagcatcga ctgaaccata tgatctcgtc ctcgatagct ttcttggctc
      241 aggtacaact gcagcagttg ctcataaaat gaaccgtcgt tatatcggta ttgagattgg
      301 cgaacatgct aaaacccatg ttgtcccacg gttgaaaaaa gtaattgaag gtgagcaagg
      361 cggtatttca aaagcagtaa attggcaagg tggtggatcg tttcgctttt gtgagttagg
      421 cgaggaagta tttgacgcat tcggttcact taatccgaac attcgttttg acgatttagc
      481 cgcccatatt tggtatttag aaaatcattt ccctttgcaa aaaaacagcg aaaaatcacc
      541 gcttgtcggc actttcaacg gcaaagccta ctatttgtta tataacggaa ttttaggcga
      601 taaacgtccg cagagtggta atgttctgac acgaaaatta atggcggagt taccatactt
      661 tgctgaattt attcaacagg gaatggatat tgtggtatat ggcgaagcgt gccgtttagg
      721 agaagtgcag ttagctgaaa agaaaattac atttaaacag attccatatg atgtaatgtc
      781 tcgttaatag taggagaaaa aaatgaaatt aaaaaattac caaaatgagg cattaaaccg
      841 tgtcagccac tatttttcgg cttgtttgca atctgatgca aaaaccgcat ttagccaaat
      901 tcaacctgaa tatagttaca agatcccaag tgaacacagt aatttgcgtg atgtgcctta
      961 tgtttgtgta cgaattccaa ctggtggcgg taaaacgttg ttagcctcgc atagtattgc
     1021 tcgtattgca aagcagtatt tggattgcga tttcccggtt accttgtggc cagtgccttc
     1081 tcgtacaatt aaaacccaaa ctacagaagc attaaaaaat ccgcaacacc cttaccgtgc
     1141 agtacttgat aaaactttta accgagaagt aatggttatt gagtctgaag attttgattt
     1201 acttcgccca caagattttg gtaataaggc gattgtaatc gtttctacta ttcaaaattt
     1261 tcgggtagaa aatcaagatg ggcgaaaaat ctatgctttt aatgaaaaat taacagctca
     1321 ttttgagcgt ttactacccc atattcaggc aaatttagaa aagatttcca ctactgatct
     1381 acaagagaac ggtttaacct ccaaacaaac gggcaaaatt aagtgttctt ttgcaaattt
     1441 gttgcgagct tatcagcctt tggtcattgt tgatgaagca cataatgctc ggacttcact
     1501 ctcttttgat gtgctatctc aactttcccc atcagctatt ttagaattta ctgctacgcc
     1561 aaacacagat agaaaagcag gaagtaatgt gttgtatcac gtttcggcaa gccatctaaa
     1621 agcagaagag atgatcaaat tgcctattgt gctgaccgaa catcacaact ggcaacaagc
     1681 aatagatgag gctgtaatca atagaggagc attagcacaa aaagcacagt atgagagcga
     1741 gtacgttcgt ccgatttatt gtttcaagct gagccgaaaa acggcaaagt aacagtagaa
     1801 gtcttaaaac agtatttaat tgatgagtta aaaattgatg aagaagagat tgccattgtg
     1861 ataggtaatc aacacgaatt agataatatc gatttattta atccacaatg caagatcaat
     1921 tacatcatca cggttgaggc attaaaagaa gggtgggatt gcccttttgc ttatattttt
     1981 tgctcagtac aaaatgtttc ctctagcaag gaagcggagc agctattagg gcgagtattg
     2041 cgaatgcctt atgctaaaca ccgtcaaatt gaagatttaa atcgtgctta tgctccgctt
     2101 tcctctaagt cgttcggcga aacggctcgt aatttacaag ataaattgat tggaatgggg
     2161 tttgaagcat tagaagtagc tgaaatgctt cgtatcccac aacaagaaaa ttggattgga
     2221 aacgaaggag atttattcta tacaccgaca acagttttag cattaacgca actccctgaa
     2281 atgtctgatt taagcgaagc aaaacgttct caacttaaaa ttaccgagca agaaggtcac
     2341 attttagtgc aagtaaaagg taatttaagt gaaaagttag aaaaagcgtt gattcgaact
     2401 gcaacaggta agaaaaaaga gcaaattgag caacagttac aaattcacca gaatagagtt
     2461 gaaatattat cttctcctgc cgaaaaaggg gaaatattta aagtaatacc tcaactgtgt
     2521 atgaatattc aaggcgaatt cgacttagct gattcacaag tgctattaga ttaccaaagt
     2581 tggaatttat tggattatcc agccaaactt gatggattta gattacaaga aaatagccaa
     2641 agttttgaaa ttgatgtgga agataaacgg gtttcttacc attttaatca gcagcaactg
     2701 agtttttctg atgattggtt aagaaatcct gtaaaaagag ggttttcttt accattagct
     2761 catcagaatt tttaccctga ttttattgta gagcttattg atggtcgaat tttaattgtg
     2821 gaatataaag gggaacaatt aaaaagtaat gaagattcta aagaaaaagc cttaattggc
     2881 aatctttggg agaaatt
//
LOCUS       DANLZC010000206         2880 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.206, whole genome shotgun sequence.
ACCESSION   DANLZC010000206 DANLZC010000000
VERSION     DANLZC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.206"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..704)
                     /locus_tag="PXE44_002461"
     CDS             complement(<1..704)
                     /locus_tag="PXE44_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5589916.1"
                     /translation="MRSMFKKFFGKNKSQSAVKSDTIFSIEQDNEGWLYPEKASQLLN
                     TELRQKYLGLLWQQVSMNRQMFEVLYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKVRQSYILPPNVAPEEQSKQRDAWTAAAIYLALVHDIGKVVVDIEITLKD
                     GSRWFAWNGQPPQPYKFKYIKGRDYHLHPVLGGFLANQLIPKSAFDWLGQYPEVISSL
                     MYAMSGHNDKAGLLSE"
     gene            complement(808..1272)
                     /locus_tag="PXE44_002462"
     CDS             complement(808..1272)
                     /locus_tag="PXE44_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HDL5589917.1"
                     /translation="MMNTQQPKFDLYQHITDRIIAALEQGTAPWLKPWSHPECNMALP
                     RNAVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRP
                     NEREKLDEEGNIVYDEEGNPEMEQHFFIAKHNLFNLDQCENLPAELYDPVIP"
     gene            1481..1744
                     /locus_tag="PXE44_002463"
     CDS             1481..1744
                     /locus_tag="PXE44_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme-like superfamily protein"
                     /protein_id="HDL5589918.1"
                     /translation="MRPVTRNNRGTSFDPRHNKYINTLATALLARWHIEQFSERNIPV
                     NDENLYMAHNIGLDGFHRALLGQSTRKDIENMRKNGMKPWMSI"
     gene            complement(1828..2853)
                     /locus_tag="PXE44_002464"
     CDS             complement(1828..2853)
                     /locus_tag="PXE44_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL5589919.1"
                     /translation="MTAGDTPHDTLKTLVGALKDTREEARKANERGDRFEQEVLRLQA
                     REAEVDSRIAGEVESRVESRLAAAWDGFNRQIEELKGRVQSGNQQQKEAEAIPVGGAS
                     SGFGTESAGQTGNVDNNGIRWVDPDDMVVTDEQGKPVTGNYSGKTKTGFPSPFKAEGE
                     SSNTGTALNNNAGTENMTGNTQIQQIKRTPYYTIPENSTLMGSTAMTALLGRVPIQGA
                     VTDPFPFKVLIGRENLIANGIDLPDVEGAIVSGTSTGDWTLSCVRSEVTSITFVFADG
                     RISNGRASGESRAVKRNQASNLTPFCLTVSLSGFNDIPHSDGNPGFLTMSAVDYVDVY
                     LMSHLAR"
BASE COUNT          803 a          648 c          561 g          868 t
ORIGIN      
        1 atctcagata aaagcccggc tttatcgtta tgcccagaca ttgcatacat cagtgaagaa
       61 atgacttctg gatactgtcc cagccaatca aacgcagact tgggaatgag ctgatttgca
      121 aggaatcctc ctagcacggg atgaagatgg tagtctcgac ctttgatgta tttgaattta
      181 taaggttgag gtggctgtcc attccaagca aaccaacgag agccgtcttt aagagtgatt
      241 tcaatatcaa ccacaacttt gccaatatca tgaaccaagg caagataaat tgcggcagca
      301 gtccaagcat ctcgttgctt tgattgttct tcgggagcta cattcggggg caaaatatag
      361 ctttgacgaa ctttagctga aaaagcaata acttctaaac cgtgatcaag cattccaccg
      421 atatgtgaat gatggtgtga ttcagaagct ggtaatagtt gaaccatctc agcgtaacgt
      481 tctattggct gctgataaag tacctcaaac atttgacggt tcattgatac ctgttgccaa
      541 agtagcccaa gatatttttg tcgtaattct gtattaagta attgagaggc tttttccggg
      601 tataaccacc cttcattatc ttgctcaata gaaaaaatag tatcggattt gaccgcactt
      661 tgagatttat ttttaccaaa aaattttttg aacattgaac gcattggaag agaccgaaaa
      721 tatgaatgct gtattgaatg aaagccgttt agccctttgc ccttttgggt aagacctttc
      781 cccttggaaa ggttactctt atccccctta ggggattaca ggatcgtaca gttccgccgg
      841 taaattttca cactggtcta aattgaacag attgtgctta gcaatgaaaa agtgttgttc
      901 catttccgga tttccttcct cgtcatacac aatatttccc tcctcatcga gtttttctct
      961 ctcattagga cggtagttca tcacaacagt agctttttcc cctttacgga tatatccgcc
     1021 gagttcattg gcttttttcg ccgtaatcca cttacactgt ttgtagccgt gttcagcggt
     1081 agcaatccaa agcaataacg tattgatacc ggaatacaga cggttagaaa ccgcattgcg
     1141 aggtaatgcc atattgcatt ccggatggct ccacggtttt aaccacggag ctgtaccttg
     1201 ttccaatgca gcaatgattc tatcagtaat atgttggtat aaatcaaatt taggttgttg
     1261 agtgttcatc atgtgtttct ccaaagtttg ggtaaaaaaa tggggaaaca cggttaccct
     1321 atggggaact tgtttccccg atgggtagtt tataaaattt caatggccac aatttttcta
     1381 gaatcatcaa agccaacaga actgcctatg gcgtagtaat tacgaatagt ctgttcatcg
     1441 gcattgatta gcagatacct atcgaggaag agagattggg atgagaccgg taacgagaaa
     1501 taatcgtgga accagttttg accctcgtca caataaatac atcaatacat tggcaaccgc
     1561 attattggca cgttggcata ttgagcaatt ctctgagcgt aatatcccag tcaatgatga
     1621 aaatctctat atggctcaca atattggctt agatggtttc catcgagcgt tattagggca
     1681 atctactcgt aaagacattg agaatatgcg taagaatggg atgaaacctt ggatgtccat
     1741 ttagctctgt tttcaaatga cctaatccag tttgttccgg agagagtacc gaaattttgc
     1801 actctttcat ccgtggtgta tcaaacttca acgtgctaaa tgagacatta aatatacatc
     1861 aacataatct accgcactca tcgttaagaa accgggattc ccatcagaat gaggaatatc
     1921 gttaaagccc gataaggaga cggttaaaca aaatggtgtt aaattacttg cttgatttcg
     1981 tttcacagct ctgctttctc cactcgctcg cccattcgaa atacgaccgt ctgcaaacac
     2041 aaaggtaatg ctcgttacct cagaacgaac gcaagatagc gtccaatctc cggttgatgt
     2101 accggataca atcgccccct caacatcagg taaatcaatg ccattggcaa ttaaattttc
     2161 tcgcccgatt aatactttaa acggaaacgg atctgtcaca gcaccttgaa taggtacacg
     2221 gcctaacaat gccgtcatcg cagtagaacc cattaaagtc gaattttcgg gaatagtgta
     2281 ataaggtgta cgctttattt gctgtatctg agtattacca gtcatatttt ctgtcccagc
     2341 attattattt aatgctgtcc cagtatttga actttccccc tctgctttaa aagggcttgg
     2401 aaagccggtt tttgttttcc cggaataatt gcctgtaacc ggtttacctt gttcatcggt
     2461 taccaccata tcatcaggat cgacccagcg aatgccattg ttatctacat tccctgtttg
     2521 tcctgctgat tcagtcccaa agccagaaga agcccctcct acagggattg cctctgcttc
     2581 tttctgctgc tgattaccac tttgaacccg acctttcagt tcttcgattt ggcgattaaa
     2641 tccatcccaa gcagcagcca aacggctttc tacacgactt tctacttcac ccgcaatacg
     2701 agaatccact tcggcttctc ttgcttgtaa gcgtaatacc tcttgctcaa agcgatcacc
     2761 acgctcattt gctttacgag cttcttcacg agtatctttt aatgctccta ccaacgtttt
     2821 taatgtatcg tgaggcgtat ctcctgcagt aatacctaat gtattcgcct cctcatcact
//
LOCUS       DANLZC010000207         2813 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.208, whole genome shotgun sequence.
ACCESSION   DANLZC010000207 DANLZC010000000
VERSION     DANLZC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.208"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            114..293
                     /locus_tag="PXE44_002465"
     CDS             114..293
                     /locus_tag="PXE44_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589920.1"
                     /translation="MFKEKGYDEFLAEKIRLGLEDMQSGNGLSLDESKARTKQLIERK
                     ARELANFEQENIIYG"
     gene            286..573
                     /locus_tag="PXE44_002466"
     CDS             286..573
                     /locus_tag="PXE44_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5589921.1"
                     /translation="MAKTLRISSRAQNDIEEILASVIEYTGFESSGIRLQEDIYQKFE
                     TIAYMPSAAGRLREDGTREAFTRRYRIVYTELENEVWIITIIHSSRLYPRI"
     gene            complement(699..2801)
                     /locus_tag="PXE44_002467"
     CDS             complement(699..2801)
                     /locus_tag="PXE44_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL5589922.1"
                     /translation="MKSFKLLPVSAAVLSVLAANAVAQTASTDAVVLDTVTVTDNQGL
                     KVQSNVVTTKKKDESTQTDLRGLLKDEPAISLGGGNGTSQYLYIRGMGQNSIDVKVDN
                     TYSDSQILYHQGRHMLDPALVKIVSVQKGAGSASAGIGQTNGAVIAKTVDALDLLKNS
                     DKNFGAKLGTGFSTNHAHNFNATLYGKGEIFDALVSGNLVRDRDYKGGKDYVNQFGTN
                     RVPYSALDKTSFLAKLGATLGDHRFVLSHSSERNEGQRLVREEFDVAPNGTKVSPYNR
                     LTVERQAPANRRITVQRTNLEWTGKNLGFAQEATANVYQLVQGRWSENETGNGYAGGV
                     DYATKTKIVTHGANVNFDSALHENVLLKYGVNYRHQEAKPHTKFNPLVKNQEKTDTGV
                     YVEAITAPVDKVTLTTGVRYDHFKFKAMDGKERSNGAFSPSVGLIYEPIEHLSLSASH
                     NYATRSPRLYDALTAHGNRKIVSIGSNTKAEKARNTEIGFNYNNGTFSFDGSYFWQDI
                     KDALGGGRDSHNPNNELIMNLGKIKNRGYELNAGYHNGGFTARIGVAHSKPRFYSPTI
                     WEVNRQTGKREEKPLLSTNPEYASAIGRTWTASLSYRFEQPNLEVGVHHRIVEKVKPE
                     DNFFVTGGTLQTNTGTGKDGYNVTDITLNWKPLKDDSVNVNFAVDNVANKKYNAHGQR
                     GQLSARGREFRAGVNYTF"
BASE COUNT          824 a          553 c          521 g          915 t
ORIGIN      
        1 agaaggtccc ctctatcaat ctgttttaaa agataattta aaaataagtt aatttgacaa
       61 ctcaaaatta aaagcagaca atgcttttaa ttatatctta ataggagcag attatgttta
      121 aagagaaagg ctatgatgaa ttcctagcag aaaaaattcg ccttggctta gaagatatgc
      181 aatcaggtaa tggtctttca ctcgatgaat caaaagcacg aaccaaacaa ctgattgaac
      241 gtaaagcaag agaactcgca aactttgaac aagagaatat catttatggc taaaacatta
      301 cgaattagca gtagagctca aaatgatatt gaagaaattt tagcgagcgt aattgaatat
      361 actggttttg aaagtagtgg aattcgctta caagaagata tttaccaaaa atttgagact
      421 atcgcttata tgccctctgc ggctggacgt ttaagagaag atggcacaag agaagcattc
      481 actaggcgtt atcgtattgt ttataccgaa ttagaaaatg aagtgtggat tattaccatt
      541 attcatagta gcagacttta tccacgcatt taaataacaa acgactaaaa tagtcggttt
      601 tttatttgca aaaaattaat aaaatatcac cgcttgtaag ctccataaaa aaaagcccga
      661 ttgtaacatc gggcgtttta gtctgaattt aagtcagatt agaatgtgta gttcacacct
      721 gcacggaatt cacgtccacg agctgataat tgaccacgct gaccgtgtgc gttgtatttt
      781 ttattcgcca cgttatccac tgcgaagttt acattcacgc tatcatcttt aagtggtttc
      841 cagtttaagg tgatatcagt tacattgtag ccgtctttgc ctgtaccggt atttgtttgt
      901 aatgttccgc ccgtaacgaa gaaattatct tcaggtttca ctttctcaac aatacggtgg
      961 tgtacgccaa cctctaaatt tggttgttca aagcgatagc ttaaagaagc tgtccaagta
     1021 cgaccgatag ctgaggcgta ttctgggtta gtacttaata atggtttttc ttcccgctta
     1081 ccagtttgtc tgtttacttc ccaaatagtt ggtgagtaga aacgcggttt gctgtgtgca
     1141 acaccaatac gagcagtaaa accaccatta tggtaacctg catttaactc ataaccacgg
     1201 tttttaattt tacctaggtt cataattaac tcattattag gattatggct atcacgacca
     1261 ccacctaatg catcttttat atcttgccag aaatagctac catcaaaact gaatgtacca
     1321 ttgttatagt tgaaaccgat ttcagtatta cgagctttct ccgctttagt attgctacca
     1381 atactaacga tttttctatt tccgtgagcc gttagtgcat cgtataaacg agggctacga
     1441 gttgcatagt tgtggcttgc acttaagctt aaatgttcaa ttggctcata gataagccca
     1501 acgcttgggc tgaatgcacc attactacgc tctttaccat ccattgcttt gaatttgaag
     1561 tggtcgtaac gtacaccggt agttaaagtt actttatcta ctggagcagt aattgcttca
     1621 acatacacgc cggtatcggt tttctcttgg tttttcacaa gagggttaaa tttagtgtgt
     1681 ggtttagctt cttggtggcg atagttcaca ccgtatttta ataatacatt ttcgtgaagg
     1741 gcagaatcaa agtttacgtt tgcaccgtgg gttactattt tagttttggt tgcataatct
     1801 acaccaccag cataaccatt acctgtttca ttctcagacc aacgaccttg tactaactga
     1861 tacacgttag ctgttgcttc ttgagcaaaa cctaagtttt taccagtcca ttctaagtta
     1921 gttctttgaa ccgtaattct acggttagct ggagcttggc gttctacagt taagcggttg
     1981 tacggagaaa cctttgtgcc gttaggtgct acatcaaatt cttcacgcac taaacgctga
     2041 ccttcatttc gctcacttga atggcttaat acaaaacggt gatctcccaa tgttgcacct
     2101 aatttagcta aaaagctggt tttatctagt gcactatacg gaacacggtt tgtgccgaat
     2161 tggtttacgt aatctttacc gcctttatag tcgcgatctc tcacaagatt acctgagact
     2221 aaagcatcaa agatttcgcc tttaccatat aaggttgcat tgaaattgtg agcgtggttt
     2281 gtgctgaagc cagtacctaa ttttgcaccg aagtttttat cgctgttttt taataaatct
     2341 aatgcatcaa cggttttagc aattactgca ccattagttt gaccaatacc ggctgaagca
     2401 ctgcctgcac ctttttgtac agaaacaatt ttcaccaatg ccggatctaa catatgacga
     2461 ccttggtgat acaaaatttg gctatctgag taagtgttat ctacttttac atcaattgag
     2521 ttttgaccca taccacgaat atagagatat tgtgatgtac cgttaccacc acctaagcta
     2581 attgccggct catcttttaa taaaccacgc aaatcggttt gggtgctttc atcttttttc
     2641 ttagttgtta ccacattgct ttgtactttt aaaccttgat tatctgttac ggtaacagta
     2701 tctaatacta ctgcatctgt tgaagcagtt tgagccactg cattagcagc aagtacgctc
     2761 aataccgcag cagaaacagg gagcaatttg aagcttttca ttttatttat att
//
LOCUS       DANLZC010000208         2748 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.209, whole genome shotgun sequence.
ACCESSION   DANLZC010000208 DANLZC010000000
VERSION     DANLZC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.209"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            294..587
                     /locus_tag="PXE44_002468"
     CDS             294..587
                     /locus_tag="PXE44_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HDL5589923.1"
                     /translation="MFKVIFMALSAIVNYLISDFLIKGVIFLSVYFLTSELLPVVIEM
                     LVSRKLIPDLNALFGQLPESMWYFLNIFQIPMAISLMISAMISRFVRRMPFIG"
     gene            597..1136
                     /locus_tag="PXE44_002469"
     CDS             597..1136
                     /locus_tag="PXE44_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589924.1"
                     /translation="MAVSAYIGIPGSGKSYEVVKSVILPAVASNRRVVSNIYGLNKEK
                     IHQYLLRKNKKLMIEELGEVIYVTNEQCLESDFLPSMDEQDSFCKAGDLIIIDEVWRI
                     WGNDKDIPKNHRSFIAEHRHFADPITGVTCDLVVINQDISQIPRFIKEGLRQLTECKN
                     TLVWGCVIVIGLMFFKERI"
     gene            1067..1630
                     /locus_tag="PXE44_002470"
     CDS             1067..1630
                     /locus_tag="PXE44_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589925.1"
                     /translation="MQKHVGLGLRNRYRVDVFQGKNLAKKNRTNSYQEKYDKKIFELY
                     KSYESDGAREAVTDSRQSIFSSFWVKVWIILLPLALFYCGYNLYIFLNGSESEVSHNT
                     EQAKFASDENHNDIVIHLLPTIGKPLSTKWRITGELKRDNKSFVILADNENRLRLEPS
                     SQFSFSGRMLQGEIDGGIVSYFSGESK"
     gene            1627..>2748
                     /locus_tag="PXE44_002471"
     CDS             1627..>2748
                     /locus_tag="PXE44_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HDL5589926.1"
                     /translation="MKIHQLGLLLMISSFSYAKNVDFKLEAVPLPKAISIIYDEVLQK
                     PYMLDPKLSNDSRVISFHTTEQQNFEQFIQRYFSNMNISVSEKKGVVYIGHIEPKQPK
                     VIKRSFVYSPIHRDVDYLASFLKDGTEGNQVFASGDKLVYYGTSEDIARVKSILKSID
                     TKSREVVVTGYVFEVQNIEKEGSGINLLAKLLSGKLGISVGYKQSYENFITVNAGNLD
                     AMFELFRTDQRFRVVSSPTLRVKSGSRGNFSVGSDVPVLSNVTYQDGRPIQSIEYRSS
                     GVIFDIQPTIKDKAIDLKIQQLSNFIKTDTGVNNSPTLVKRDLVTDVTVKSGDVIVLG
                     GLAENKISNGETSFSLLPKGWLSGKSNENTKTDIIVLLQV"
BASE COUNT          916 a          382 c          548 g          902 t
ORIGIN      
        1 caagtctgat cttctacacc tgatattaat ttgcctgatc tggaagttcc tacggcggaa
       61 tcaattatat ccccttttaa aaaattgcta ccagatttac agaattttga actgaataaa
      121 cgaaccgcac aatgccctgt ttggacattt aatattgaat acttgagatt tcattatgaa
      181 cttagagaac attgcgattt gatagaacca cataaagaaa tcttaaagct attattcggc
      241 attatttggg cgtttattgc tttacgttat gtattaagtg cttaggagga taaatgttta
      301 aagtgatttt tatggcacta tcagcaattg tgaattattt gatttcagat tttttaatta
      361 agggtgtcat atttttatct gtctattttt taacttctga actacttcct gtagtaatag
      421 agatgcttgt gtctagaaaa ttgatacctg atttaaatgc gttatttggg caattgcctg
      481 aaagtatgtg gtatttttta aatatttttc agataccaat ggctatttct ttaatgattt
      541 cagcaatgat ttcacgtttt gttagacgta tgccatttat tggatagagg agtgctatgg
      601 ctgtttcagc gtatataggg attcctggaa gcggcaaatc gtatgaagtg gttaagtcag
      661 taattttacc tgctgttgct tcgaatcgaa gagttgtttc caacatttat ggattaaata
      721 aagagaaaat tcatcaatat ttactgcgta aaaataaaaa attaatgatt gaagagcttg
      781 gtgaagtcat atatgtaacg aatgaacaat gcttggaatc ggatttttta ccatcaatgg
      841 acgaacaaga ttcattttgc aaagctgggg acttgataat tattgatgaa gtttggcgta
      901 tttggggaaa cgacaaggat attccaaaaa atcatcgttc atttattgct gaacatcgac
      961 attttgcaga tcctataaca ggtgtaactt gtgatttagt cgtaataaat caagatattt
     1021 ctcaaattcc aagatttatt aaagaaggat tgagacaact taccgaatgc aaaaacacgt
     1081 tggtttgggg ttgcgtaatc gttatcgggt tgatgttttt caaggaaaga atttagctaa
     1141 aaagaaccgg acaaattctt atcaagaaaa atatgataaa aaaatatttg agctttataa
     1201 atcttatgaa tctgatggtg caagagaagc ggttacagat agtaggcaat ctatattttc
     1261 atctttttgg gtcaaggtat ggattattct tttaccattg gcattatttt attgtggcta
     1321 taacctttac attttcctaa atggttcaga gagtgaagta agccataata ctgaacaggc
     1381 taaatttgct tctgatgaaa accataacga tatagtaatt catttacttc ctactattgg
     1441 aaaaccttta tcaactaaat ggcgaataac aggggaacta aaaagggata ataaatcatt
     1501 tgtgatctta gctgacaatg aaaaccgttt aagacttgag cctagtagcc agtttagttt
     1561 tagcggtcga atgttgcaag gagagataga tggtggaata gttagttatt tttcgggaga
     1621 atctaaatga aaatccatca attagggcta ttgctcatga ttagctcatt ttcttatgct
     1681 aagaatgttg attttaaatt agaagctgtg ccgttaccga aagcaatatc aataatatac
     1741 gatgaagtgc tacaaaagcc atatatgctt gatcctaaat tatctaatga ttcaagggtt
     1801 attagctttc atacaacaga gcaacagaat tttgagcagt ttattcaacg gtatttcagc
     1861 aatatgaata tcagtgtatc agagaaaaaa ggtgttgttt atattggaca tattgaacca
     1921 aaacagccga aagtaataaa acgtagtttt gtgtattcgc ctattcatcg tgatgttgat
     1981 tatttggcta gcttcctgaa agatggaaca gaaggaaacc aagtttttgc aagtggcgac
     2041 aagcttgtat attatggtac ttctgaagat attgcacgag tgaaaagtat cttgaaatca
     2101 atcgacacaa aaagccgtga agtagtggta actggttatg tttttgaagt gcagaatatt
     2161 gaaaaagagg gtagtggcat aaacttgtta gcgaaattgc tatcaggaaa attaggcatt
     2221 agtgttggtt acaagcaaag ttatgaaaac tttatcaccg tgaatgcagg aaatttagat
     2281 gcaatgtttg aattgtttcg cacagatcag cgttttaggg ttgtcagcag tccgacatta
     2341 agagtaaaat caggctcaag aggtaatttt tcagtaggct ctgatgtccc tgtattatcg
     2401 aatgtaacct atcaggacgg aaggcctatt cagtcgattg aataccgttc aagcggtgta
     2461 atatttgata ttcagccaac gataaaagat aaagcgattg atttaaaaat tcaacaacta
     2521 tcaaatttca tcaaaacaga taccggtgta aataatagcc caacgttggt aaagcgtgat
     2581 ctggtaacag atgttacggt taaaagcggt gatgttatcg ttttaggtgg gttagctgaa
     2641 aataaaatat ctaatggcga aaccagtttt tcattgctac ctaaaggttg gttaagtgga
     2701 aaatcaaatg aaaatactaa aacagatatt atagttctgc tacaagta
//
LOCUS       DANLZC010000209         2744 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.210, whole genome shotgun sequence.
ACCESSION   DANLZC010000209 DANLZC010000000
VERSION     DANLZC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.210"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            310..1785
                     /gene="msr(E)"
                     /locus_tag="PXE44_002472"
     CDS             310..1785
                     /gene="msr(E)"
                     /locus_tag="PXE44_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(E)"
                     /protein_id="HDL5589927.1"
                     /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKS
                     SLLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSG
                     GEETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLD
                     MVVDKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQ
                     QANRLDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPE
                     HLRSIRFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTG
                     KTSLLKMISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVL
                     ASMGIGANDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETM
                     MKSYAGTIIFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC"
     gene            1841..2725
                     /locus_tag="PXE44_002473"
     CDS             1841..2725
                     /locus_tag="PXE44_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="HDL5589928.1"
                     /translation="MTIQDIQSLAEAHGLLITDKMNFNEMGIDFKVVFALDTKGQQWL
                     LRIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAE
                     TYEIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLV
                     KSEIGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAH
                     IDDPAIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFAL
                     ETQNESLIVGAKAQLGVI"
BASE COUNT          937 a          441 c          574 g          792 t
ORIGIN      
        1 aaaataagcc ttatatccaa gcataaaaca aggttgtcta gacttctttt aacagtaaag
       61 ttatcataaa actgaatttt attttttagg taagtttatg cattctatcc gcattcgtta
      121 agacacaact atttgcatag tgacactatt ttataatggt gggcttttgt tgtgtcttta
      181 agaatatatg cggatatata aagtaaaagt atgcttaatt tataagtatg cttttagtgc
      241 atagtttcca gttataactt aattgactag ctattaaaaa tttcaagagc taaacaggag
      301 taaataaaaa tgagtttaat tattaaagcg agaaacatac gcttggatta tgctgggcgt
      361 gatgttttgg atattgatga attggaaatt cactcttatg accgtattgg tcttgtgggt
      421 gataacggag caggaaagag tagtttactc aaagtactta atggcgaaat tgttttagcc
      481 gaagcgacat tacagcgttt tggtgatttt gcacatatca gccaactggg cggaatcgaa
      541 atagaaacgg tcgaagaccg ggcaatgtta tctcgccttg gtgtttccaa tgtacaaaac
      601 gacacaatga gtggcggaga ggaaactcgt gcaaaaattg ctgccgcatt ttcccaacaa
      661 gtacatggca ttctagcgga tgaaccaacc agccaccttg atctcaatgg aatagatcta
      721 cttattggtc aacttaaagc atttgatgga gcattacttg ttatcagtca tgaccgatat
      781 tttcttgata tggttgtaga caagatatgg gagttaaaag acggtaaaat tacggaatat
      841 tggggtggtt actcggatta cttgcgtcaa aaagaagaag agcgacaaca ccaagccgta
      901 gaatatgagc tgatgatgaa ggaacgggag cgattagaat ctgctgtgca agaaaaacgc
      961 cagcaagcta atcgattaga caataagaaa aaaggagaaa aatccaaaaa ctctaccgaa
     1021 agtgctggac gacttgggca tgcaaaaatg actggcacca agcaaagaaa actgtatcag
     1081 gcagctaaga gtatggaaaa gcgtttggct gcattagaag atattcaagc accagagcat
     1141 ttgcgttcta ttcgttttcg tcaaagttca gccctagaac tgcacaataa gttcccgatt
     1201 acggcagatg gtctgagctt aaaatttggt agccgtacta tctttgatga cgctaacttt
     1261 ataataccgc ttggcgctaa agtcgctata actggatcga atggaacagg gaaaacgtcc
     1321 ttgttaaaaa tgatatcaga acgtgctgat ggattaacca tatctccaaa agctgaaatt
     1381 ggctacttta cacaaacagg atataaattt aacacgcata aatctgtgct ctcctttatg
     1441 caggaagagt gcgagtacac agttgcggaa attcgtgcag tattggcttc aatggggatc
     1501 ggagcgaatg atattcaaaa aaacttatcc gacttatcgg gaggtgaaat catcaaactg
     1561 cttttatcca aaatgctttt aggaaaatat aatattttgc ttatggatga accaggaaac
     1621 tatcttgacc taaaaagtat tgccgcatta gaaacaatga tgaagtccta tgcaggaact
     1681 attatcttcg tatctcatga caagcaattg gtcgataata ttgctgacat tatctacgag
     1741 atcaaagacc acaaaatcat caagactttt gagagagatt gttaatgata gccaatctaa
     1801 tccgaacatt aattattgaa ctctttaaag gaaattaaaa atgacaattc aagatattca
     1861 atcacttgct gaagcacacg gcttgttgat tacggacaaa atgaatttca atgaaatggg
     1921 cattgatttt aaggtcgttt ttgctcttga tacaaagggg caacaatggt tgctgcgtat
     1981 tcctcgtcgt gatggcatga gggaacaaat caagaaagaa aaacgcattt tagaattggt
     2041 aaaaaaacat ctttctgtag aggttcctga ttggagaatt tcatctacag aattagtggc
     2101 ttatcccata cttaaagata atcctgtttt aaatttggat gctgaaacct atgaaataat
     2161 ttggaatatg gacaaagata gcccgaaata cataacatct ttggcaaaaa ccttatttga
     2221 aatccatagt attcctgaaa aagaagttcg ggaaaatgat ttgaaaatta tgaaaccttc
     2281 agatttaaga cctgaaatag caaacaattt gcagttagta aaatctgaaa ttggtataag
     2341 tgagcaattg gaaacccgct acagaaaatg gttggataat gatgttctat gggcagattt
     2401 cacccaattt atacatggcg atttatatgc tgggcatgta ctagcttcaa aggatggagc
     2461 tgtttcaggc gttattgatt ggtcaacagc ccatatagat gacccagcga ttgattttgc
     2521 tgggcatgta actttgtttg gagaagaaag cctcaaaact ctaatcatcg agtatgaaaa
     2581 actagggggt aaagtttgga ataaactata tgaacagact ttagaaagag cagcggcctc
     2641 tcctttgatg tatggtttat ttgccttaga aactcaaaat gaaagcctta tcgttggagc
     2701 aaaagctcag ttgggagtta tataatttaa aaatatgatt gctg
//
LOCUS       DANLZC010000210         2744 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.211, whole genome shotgun sequence.
ACCESSION   DANLZC010000210 DANLZC010000000
VERSION     DANLZC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.211"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(56..2683)
                     /gene="alaS"
                     /locus_tag="PXE44_002474"
     CDS             complement(56..2683)
                     /gene="alaS"
                     /locus_tag="PXE44_002474"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL5589929.1"
                     /translation="MKTTSDIRQSFLEFFQTKGHTIVPSSSLVPENDPTLLFTNAGMN
                     QFKDVFLGLEKRPYSRATTAQRCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYF
                     KQDAIKFGWEFLTSPQWLGLPKEKLYVTVYETDDEAYDIWHKEVGVPSEHIIRIGDNK
                     GAPYASDNFWAMGDTGPCGPCTEIFYDHGPEHWGGLPGSPEEDGDRYIEVWNIVFMQF
                     NRLADGTMEKLPKPSVDTGMGLERMTAVMQHVNSNYETDIFQTLIKEVATLLNVKDLD
                     NKSLRVVADHIRACSYLIADGVLPSNEGRGYVLRRIIRRAVRHGNILGAKEAFFYKLV
                     PTLATVMGQAGEVLTQKQAHIQKTLKAEEEQFARTLERGLNLLEEALAKVENNTLSGE
                     VAFKLYDTYGFPLDLTADVCRERDIKIDEDGFNAEMTAQRERAKVSSSFGTDYNNVIK
                     VDSKTCFSGYTETSLNDATVIGLFSNGKSVESIQSGENAVVILDKTPFYAEMGGQVGD
                     SGQISAEICNFEVVDTQKYGQVFGHIGQLTSGSLSVGDKVNTIVDRERRHAITLNHSA
                     THLLHSALRQVLGDHVAQKGSLVSENILRFDISQPEAITKSQLEEVERIVNAKVRENI
                     QVIIEEMDIESAKAKGAMALFGEKYGEVVRVVEMSDFSIELCGGTHVKQTGDIGLFKI
                     TSEGAVAAGVRRIEAVTGENAIAWLHTLQQAVQQSAELLKADSHSFVEKIVQLQEKAK
                     RTEKELQQLKDKLAAQAGSDLAKQATQINGVNVVIQQLENVEPKALRTMVDDLKNQLG
                     SAVVVFATTAEDKVNLIVGVTKDLTDKVNAGQLVGAMAEKVGGKGGGRPDMAMAGGSE
                     PQHLLQALSLAQEWITAKL"
BASE COUNT          759 a          629 c          478 g          878 t
ORIGIN      
        1 acttaaatat agagtaaagt tttaaaggcg agttcagctc gccttgttat tatttttaaa
       61 gttttgctgt aatccattct tgagcaagtg acaatgcttg caataaatgt tgtggttctg
      121 agcctccggc cattgccata tcaggacgtc cgcccccttt acctcccact ttttcagcca
      181 ttgcaccaac taattgacca gcattgactt tatcggttaa atctttggtc acacctacaa
      241 tcaaattgac tttatcttcc gccgtagtcg caaatacaac aactgcactg cctaattgat
      301 tttttaggtc atcaaccatc gttctcaatg cttttggttc aacattctct aattgttgaa
      361 tcactacatt cacgccattg atttgggtag cttgtttggc taaatctgaa cctgcttgtg
      421 ctgcaagttt atcttttagc tgttgaagct ctttttctgt acgtttagcc ttctcttgta
      481 attgaacaat tttctcaacg aatgaatgac tgtctgcttt taacaattca gcactttgtt
      541 gaacggcttg ttgtaaggta tgcaaccaag caatcgcatt ttcaccagtg acagcctcta
      601 tacggcgtac acctgctgct accgcacctt cagaggtaat tttgaataag ccaatatcac
      661 cggtttgttt aacgtgtgtg ccgccacaaa gttcaatcga gaaatctgac atttccacta
      721 cacgcactac ttcgccatat ttttcaccaa ataacgccat tgcacctttc gcttttgcag
      781 attcaatatc catttcttca atgattacct gaatattttc acgcacttta gcattcacga
      841 tacgttcaac ttcttcaagt tgtgatttag taattgcttc cggctgagaa atatcaaaac
      901 gtaaaatatt ttctgacact aatgagcctt tttgagcaac gtggtcgcct agcacttggc
      961 gtaacgcaga atgcagtaag tgggtagcac tgtggtttaa tgtaatagca tgacgacgct
     1021 ctctatctac gatcgtatta actttgtcgc caacagaaag tgagccggaa gttaattgcc
     1081 caatatgccc gaatacttga ccatattttt gcgtatctac tacctcaaaa ttgcaaattt
     1141 ctgcggaaat ttgaccgcta tctcccactt gtccgcccat ttctgcatag aatggggttt
     1201 tatctaaaat cactaccgca ttttcacccg attgaataga ctcaaccgat tttccattac
     1261 taaataaccc aattacagta gcatcattta agctggtttc agtatatcca ctaaagcatg
     1321 ttttactgtc cactttaatc acattgttat aatctgtgcc aaagctactg ctgactttcg
     1381 cacgctcacg ttgtgcggtc atttctgcat tgaagccgtc ttcgtcaatt ttaatatcac
     1441 gttcacggca aacatctgcg gttaaatcta acgggaagcc ataagtatca taaagtttga
     1501 acgcaacttc gccagataag gtgttatttt caaccttcgc taatgcttct tctaataaat
     1561 tcaatccacg ctcaagagta cgggcaaatt gctcttcttc tgctttgagt gtcttttgaa
     1621 tatgcgcttg tttttgggtc agcacctcac cagcttgccc cattaccgtt gcaagtgttg
     1681 gcaccaattt atagaaaaag gcttcttttg cacccaaaat attaccgtga cgaaccgccc
     1741 ggcggataat acggcgtaat acatagcctc ttccttcatt tgaaggtaaa acaccatcgg
     1801 caatcagata ggaacaagca cgaatatggt ctgccactac acgtaaagat ttattatcta
     1861 aatctttcac attaagcaaa gtagcgactt ctttaattaa agtttggaaa atatccgttt
     1921 cgtagttgga gtttacgtgt tgcattaccg cagtcatacg ttctaacccc atccctgtat
     1981 ctacagaagg cttaggcagt ttttccatcg taccgtctgc taaacgattg aactgcataa
     2041 atacaatatt ccatacctca atatagcggt cgccatcttc ttctgggcta cccggtaagc
     2101 caccccaatg ttcaggcccg tgatcataga aaatctcggt acaagggcca caagggccgg
     2161 tatcgcccat cgcccagaag ttatctgaag cataaggtgc gcctttatta tcgccaatgc
     2221 gaataatatg ttccgatggt acacctactt ctttatgcca aatatcgtag gcttcatcat
     2281 cagtttcata cacagttaca tataattttt cttttggtaa gccaagccat tgtggagaag
     2341 ttaaaaactc ccaaccgaat ttgattgcat cttgcttgaa gtaatcacca aagctgaaat
     2401 tccccatcat ttcaaaaaaa gtatgatggc gagcggtata acctacgttt tctaaatcat
     2461 tgtgtttacc acctgcacgt acacaacgtt gtgccgtagt cgctctgcta taagggcgtt
     2521 tttctaaccc aagaaaaaca tctttgaatt ggttcatgcc cgcattggta aataataatg
     2581 tcggatcatt ctcgggaaca agagagctgc ttggaacaat cgtatgtcct ttagtttgga
     2641 aaaactctag aaaagattgt ctgatatctg aagttgtttt cattaaaaat cactcttttt
     2701 gtaaatagaa gttaaacatt tagttatctt gctattttac acat
//
LOCUS       DANLZC010000211         2695 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.212, whole genome shotgun sequence.
ACCESSION   DANLZC010000211 DANLZC010000000
VERSION     DANLZC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.212"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            77..835
                     /locus_tag="PXE44_002475"
     CDS             77..835
                     /locus_tag="PXE44_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5589930.1"
                     /translation="MIPLERQREILRLINNNGVASINFLAEQLKVSHMTIRRDIQKLE
                     EQGKVSLVSGGVQSLERLIMELPHDDKSMLFQEQKSAIGSLASQLIPSNTTIYLDAGT
                     TTLEIAHRIIDRNDLLVVTNDFVIAHYLSNSGKCDLIHIGGSVCKENLSTTGSLAAEF
                     LKNLSIDMAFISTSSWNLKGLTTPNENKIPVKKAILEASKQNILVTDSSKYGKVATFF
                     VCPLTVFDQIICDKGLFENVRDALKGLSIKLDLG"
     gene            complement(975..2366)
                     /gene="ffh"
                     /locus_tag="PXE44_002476"
     CDS             complement(975..2366)
                     /gene="ffh"
                     /locus_tag="PXE44_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL5589931.1"
                     /translation="MFENLSDRLSKTLRNITGKGRLTEDNIKDTLREVRMALLEADVA
                     LPVVRDFINKVKERALGVEVNKSLTPGQEFLKIVQSELETAMGEANEELNLAAQPPAV
                     ILMAGLQGAGKTTSVGKLAKFLKERHKKKVLVVSADVYRPAAIKQLQTLAEALKVDFF
                     PTETSQKPVAIAEAALKHAKLNFFDVLIVDTAGRLHVDGEMMEEIQQIHAVLNPIETL
                     FTVDAMTGQDAANTAKAFNEALPLTGVILTKVDGDARGGAALSIRQITGKPIKFLGVG
                     EKTDALEPFHPDRVASRILGMGDVMSLIEDIQRSVDQEKAEKIAAKFKKGDEFTLEDF
                     REQLIEMKKMGGMMSMLDKLPGAKNLPDHVKSQVDDKMFMKMEAIINSMTLKERANPD
                     IIKGSRRRRIALGSGTQVQDVNKLLKQFDDMQRMMKKMRKGGMAKMMRGVKGMMGGGL
                     GGLGGLGSMFGKR"
BASE COUNT          814 a          557 c          520 g          804 t
ORIGIN      
        1 atgtgaattt ttgtgatttt aattttggaa actaaatctg atataatctc ttttaacaat
       61 taaaataggt taaaaaatga tccccttaga gagacaacga gaaatactac gacttattaa
      121 taataatggt gtcgcaagta ttaattttct ggcagaacag ctaaaagtat cacatatgac
      181 aattcgccgt gatattcaaa agctggaaga gcaaggtaag gtatccctag tatctggtgg
      241 agttcaatct cttgaacgat taatcatgga attaccacac gatgataaat caatgttatt
      301 ccaagagcag aaatcagcta taggatcttt agcatcacag ctaattccct caaacacaac
      361 tatttatctt gatgctggaa ctactacatt agaaatagct catcggataa tcgatagaaa
      421 tgacttacta gttgtaacaa acgattttgt tattgctcac tatcttagta attcaggaaa
      481 atgtgattta attcatatag gaggaagtgt ctgtaaagaa aacctatcga caactggttc
      541 acttgcagca gaatttctga aaaacctatc aatcgatatg gcatttatat ctacttcatc
      601 ctggaattta aaaggtctaa caacgccgaa cgaaaataaa atccccgtta aaaaagcaat
      661 tttagaggca tccaagcaaa acattttagt gacagactct tcaaaatatg gaaaagtcgc
      721 aacttttttt gtttgccctc ttactgtatt tgaccagatt atttgtgata aaggattatt
      781 tgagaatgta cgagacgcac tgaaagggct aagtattaaa ttagatttag gataatcttc
      841 ttaccaattt tttctgagaa cgacaagcaa tcaaaaagta ttatttttgc aaataagata
      901 ttaataggga aagttaacaa tcctagcaac ggattgttaa ctgattttcg gctggtacta
      961 ataaaatagg aatattaacg tttaccaaac atactgccta agccaccgag accgcctaga
     1021 ccaccaccca tcataccttt aacaccacgc atcatttttg ccattccgcc tttacgcatt
     1081 tttttcatca tacgttgcat atcatcaaac tgcttgagca gcttatttac atcttgcacc
     1141 tgtgtgccgg agccaagtgc gatacggcgg cggcgtgagc ctttaataat atccggattt
     1201 gcccgctctt ttaaggtcat agaattgatg atggcttcca ttttcatgaa cattttatca
     1261 tcaacctgac ttttaacgtg gtcaggcagg tttttcgccc ccggtagttt atcgagcatc
     1321 gacatcattc cacccatttt tttcatttcg ataagctgct cacggaaatc ttccaaggta
     1381 aattcatcgc cttttttgaa tttggcggca attttttccg ctttttcttg atcaaccgag
     1441 cgttgaatat cttcaattaa agacattaca tcgcccatac ctaaaatgcg ggaagccaca
     1501 cggtcagggt ggaacggctc aagggcatca gttttttcac ccacacctaa gaatttaatc
     1561 ggtttaccgg tgatttggcg aatagaaagt gccgcaccgc ctcgggcatc gccatcgact
     1621 ttggtaagaa taacaccggt aagcggcaac gcttcgttaa aggcttttgc ggtgttggcg
     1681 gcgtcttgac cggtcatcgc atcaaccgta aatagcgttt caatcggatt cagcacagcg
     1741 tggatttgtt ggatctcttc catcatttcg ccatcaacgt gtaaacgacc ggcagtatcc
     1801 acaatcaata catcaaagaa attaagctta gcgtgcttca gtgccgcttc tgcaatcgct
     1861 accggttttt ggctggtttc ggtcgggaag aagtccactt ttaacgcttc agcaagggtc
     1921 tgtagctgct tgatagcggc agggcggtaa acatcggcag aaactaccaa cacctttttc
     1981 ttgtggcgtt ctttcaggaa ttttgccaat ttgccgacag aggtggtttt acccgcacct
     2041 tgcaaacctg ccattaaaat taccgcaggc ggttgggccg caagattcag ctcctcgttg
     2101 gcttctccca ttgccgtttc taattcgctt tgtacgattt ttaagaactc ttgccccggc
     2161 gtgaggcttt tgtttacctc aacccctaac gcacgctctt tgaccttgtt gatgaaatca
     2221 cgcacaacag ggagggcaac atccgcctcc aacaatgcca tacgaacttc acgcagggta
     2281 tctttaatat tatcttccgt taggcgacct ttgcccgtaa tgttacgcag cgttttagac
     2341 agtctgtcag agaggttttc aaacatatca gctttctcaa tttgcaaaat tttaggaaaa
     2401 aagtaccgct tgtgagcgga aagttgccgc cattatacaa aaaaaagcgt tacggttata
     2461 gggaaataaa aaaacggcat agcgattaaa ctatgccgta aaatttgacg atattcgagg
     2521 aatcattatt tagttatatt gtgcgatcat aatactccga attgcgtata aaagtgaagt
     2581 gatattttca aatttatgtt agaaatgaga ggtaaattac aaaattaaag gggatattat
     2641 tgtttgaaaa aataagagcg aaaagaaaac cctgatgatt gctcatcagg gcttc
//
LOCUS       DANLZC010000212         2692 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.213, whole genome shotgun sequence.
ACCESSION   DANLZC010000212 DANLZC010000000
VERSION     DANLZC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.213"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..1082
                     /locus_tag="PXE44_002477"
     CDS             <1..1082
                     /locus_tag="PXE44_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL5589932.1"
                     /translation="AASASATTVYDAEGSKVDFDGSLRIVLDRTNSKELDNHNVVKTK
                     KDAHTGIRNTGSRFGVRVKHDLAQDFYALGRVEFRFNKNTSDDGFGDLYTKRAYVGLG
                     SKQYGELTFGRQVTIADDLSQSVDYNYGINPKGDYIQTDGNQVIRYNYRGVENLHVSA
                     NYNFAQDRKDGKVLANKLQNAYGIGALYTVDNWDLRAAYGHTNYKTATDSTKHRKDGV
                     LGSVGYKFGDLKLSVDTGYSREKGLAVTDDNGVTTFYNGVKSNAFYVSPNFEYQVVPA
                     SKVYGNYLYERVKSKSNGSTYAAGKEKTHGFLLGVDYQFHKQVVAFVEGSYTRTKDYD
                     KVTNGYEYSGKSTNKAIGVGMRVYW"
     gene            complement(1235..2599)
                     /gene="glmU"
                     /locus_tag="PXE44_002478"
     CDS             complement(1235..2599)
                     /gene="glmU"
                     /locus_tag="PXE44_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL5589933.1"
                     /translation="MTQLSVVILAAGKGTRMYSDLPKVLHPVAGKPMVKHVIDTAKQL
                     DAQQIHLIYGHGADLLKARLSEEPVNWVFQAEQLGTGHAMQQAAPFFADDENIVMLYG
                     DAPLITKATLERLIAAKPENGIALLTVELENPTGYGRIIRENGSVVAIVEQKDANEAQ
                     LKVKEVNTGVMVASGASFKKWLKNLNNNNAQGEYYITDVIAMANQEGFKVQAVQATEF
                     MEVEGANNRLQLAALERFYQKREAEKLLLAGVTIIDPSRFDLRGTVTHGKDVQIDVNV
                     ILEGEIKLGNNVKIGAGCVLKNCEIGDNVEIKPYSVIEDSIVGAKSAIGPFSRLRPGA
                     ELAEETHIGNFVEIKKATIGKGSKVNHLTYVGDAEIGKECNIGAGVITCNYDGANKFK
                     TIIGDNVFVGSDSQLIAPVTIASGSTIGAGATVTKDIAENELVISRVPQRHIQGWQRP
                     TKKK"
BASE COUNT          765 a          500 c          531 g          896 t
ORIGIN      
        1 tcgcagcgtc agcatcagca accaccgttt acgatgcaga aggttcaaaa gtagattttg
       61 atggttcttt acgtattgtt cttgatagaa caaattctaa agagctagac aatcataatg
      121 ttgttaaaac taaaaaagat gcacacacag gaatccgtaa cactggttca cgttttggtg
      181 taagagtaaa acatgattta gctcaagatt tctatgcatt aggtcgtgtt gagttccgtt
      241 ttaacaaaaa taccagtgat gatggttttg gtgatttata tactaaacgt gcttatgttg
      301 gtttaggtag taagcaatat ggtgaattaa cttttggtcg tcaagtaacg attgctgatg
      361 atttaagtca atcagtagat tacaactacg gtattaatcc aaaaggtgat tatattcaaa
      421 ccgatggtaa ccaagttatt cgttataact accgtggtgt tgaaaactta catgtaagtg
      481 caaactataa ctttgcacaa gatcgtaaag atggtaaagt attagcaaat aaacttcaaa
      541 atgcatacgg cattggtgca ttatacactg tagataactg ggatttacgt gcagcttatg
      601 gtcatacaaa ctacaaaaca gctactgata gcactaagca ccgtaaagat ggtgtattag
      661 gttctgttgg ttataaattt ggtgacttaa aattaagtgt tgatacaggt tattctcgtg
      721 agaagggttt agctgttacg gatgataatg gtgtaactac attctataat ggtgttaaat
      781 caaacgcttt ctatgtatct ccaaactttg aatatcaagt agtgcctgca tctaaagtat
      841 atggtaacta cttatatgag cgtgtgaaat ctaaatcaaa tggttctaca tatgctgctg
      901 gtaaagaaaa aactcatggt ttcttacttg gtgttgatta ccaattccat aaacaagtag
      961 ttgcttttgt tgagggttct tatactcgta ctaaagacta tgataaagta actaatggtt
     1021 atgaatactc aggcaaatca actaacaaag ctatcggtgt tggtatgcgt gtatactggt
     1081 aatcaattga ttttctaaat tgataaaccc ctaacaatat ttattgttag gggttttgtt
     1141 ttaaatatat tgttcattat cctttttaaa gtgtatggct ttgctgttat tggcaaagcc
     1201 aatttttaaa tataataact tttgaccact ggcactattt ctttttagtc gggcgttgcc
     1261 agccttgaat atggcgttgt ggtacacggc taattaccag ttcattttcc gcaatgtctt
     1321 tagtgactgt tgcaccggca ccaatggttg aaccgctggc aatcgtgacc ggggcaatga
     1381 gttggctgtc tgagccgaca aatacgttat cgccaataat ggttttgaat ttattcgcac
     1441 catcatagtt acaagtgatt acacctgctc cgatgttaca ttctttgccg atttccgcat
     1501 cgcctacata agtgagatga tttacttttg aacctttgcc gatggtagct tttttgattt
     1561 ccacaaagtt accgatatgg gtttcttccg ctaattctgc ccccgggcgt aagcgagaga
     1621 atggaccgat agcggatttt gccccgacaa tcgaatcttc aatcacagaa taaggtttaa
     1681 tttcaacatt atcgccaatt tcgcagtttt tcagtacgca gcctgcacca atttttacat
     1741 tgttaccgag tttgatttca ccttctagaa tgacattcac atcgatctga acatcttttc
     1801 cgtgggtgac agtgccacga agatcaaaac ggcttgggtc gataatagtt acaccagcca
     1861 ataacagttt ttccgcctca cgtttttggt agaaccgttc aagtgcagca agttgcaaac
     1921 ggttgtttgc cccttctact tccataaatt cagttgcctg aaccgcttgt actttaaagc
     1981 cttcttggtt tgccattgcg atcacatcag tgatgtaata ttcaccttga gcattgttgt
     2041 tatttaggtt tttcaaccat tttttgaagc tcgcaccgct tgctaccatc acgccggtat
     2101 tcacttcttt gactttaagc tgtgcttcat tggcatcttt ttgctcaaca atcgctacca
     2161 cagaaccatt ttcacgaata attcgtccgt agcctgtcgg gttttccaat tcaacggtta
     2221 gtaatgcaat accattttcc ggtttggctg cgattaaacg ctctaaagtg gcttttgtga
     2281 ttaggggggc atcaccataa agcattacaa tgttttcatc atcagcgaaa aaaggagctg
     2341 cttgttgcat tgcatgtcct gtgcctaatt gttcggcttg gaatacccag tttacaggct
     2401 cttcgcttaa acgtgctttt aacagatcgg caccgtgtcc gtaaattaaa tgaatttgct
     2461 gggcatctag ctgtttggca gtatcaataa cgtgttttac catgggttta ccggcaactg
     2521 ggtgtaacac tttgggtaaa tctgaataca tacgagtgcc tttaccggca gctaaaataa
     2581 caacgctcag ttgagtcata tagaatcctt atttgactaa atagatgaag gcttatagta
     2641 acgtgtaaat tgctataagt gatatttttg taatatttta acctttttat aa
//
LOCUS       DANLZC010000213         2690 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.214, whole genome shotgun sequence.
ACCESSION   DANLZC010000213 DANLZC010000000
VERSION     DANLZC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.214"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            139..579
                     /locus_tag="PXE44_002479"
     CDS             139..579
                     /locus_tag="PXE44_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL5589934.1"
                     /translation="MTACKNGLGFLVLRLFLAYEFFEAGLSKLNGENWFASIQESFPF
                     PFNVLPADLSWAIASGAEILLPILLTLGLFTRFSALGLMILTAVAWYAVHAGNGYNVC
                     NNGYKMALIYLVMLVPLLLQGAGSLSLDFLLQKKSPTKRWLKFL"
     gene            605..892
                     /locus_tag="PXE44_002480"
     CDS             605..892
                     /locus_tag="PXE44_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589935.1"
                     /translation="MKKATLMALAGALTMATATVNAETKSVKADACMEVKDGKCVKTK
                     ATEGKCGEGKCGATSSEPKATEGKCGEGKCGATAPKAAEGKCGEGKCGASK"
     gene            993..1916
                     /locus_tag="PXE44_002481"
     CDS             993..1916
                     /locus_tag="PXE44_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HDL5589936.1"
                     /translation="MLKFTGAGLGYRRDLADGFLSLTDNNIINFMEVAPENWLKMGGQ
                     ARYNFDKVAEKIPVIIHGLSLSLGGQAPLDMTLLKGIKNMQQQYNSFFFSDHLSYCEC
                     EGHLYDLLPMPFTDEAVHHVANRIKQVQDYLGMQISLENTSYYLHSPTSTMNEVEFLN
                     AIAQEADCGIHLDVNNIYVNGVNHGLLDPFVFIDQVDKSRVNYIHIAGHDEDHGAAED
                     VKMTEGESFNMVKGELRHLPTLLVDTHGEAVQKTVWDLLEYTYEHLPHIPPTLLERDF
                     NFPPFQELISEVQHIMSLQKKYAKTRLKNAA"
     gene            1906..2646
                     /locus_tag="PXE44_002482"
     CDS             1906..2646
                     /locus_tag="PXE44_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HDL5589937.1"
                     /translation="MPLKLVEEHKPLLKEIQAEFANAIRHNDVKLMSGNISPQRLGVY
                     SRLVRNNTLGFIDRCYVQLPQHLSPEEWVAVKEKFIREGKAHSPFFQDIAGEFLNFCR
                     ENGCMPPHLLELMDFENAQLLAEVSMATVPSEFEWDNDTIMQFSGAAELRQYAVNFIR
                     SEFKQFDFEPTNVLIWRDTEFGIYYNRLEELDFFLLSSLQEGANSLNGLLAELSEFTE
                     QNLELKALLRQLWEKWVEAEVIYPVYPE"
BASE COUNT          803 a          532 c          579 g          776 t
ORIGIN      
        1 ctataaaaat aggttataat ttttcacaca ttttttgtaa taaacgtata cttattctgt
       61 ctaataatca catttttagt taggagcatt tttatgtcat cattttcgtc atcattttca
      121 actcagtcag cttgctcaat gacagcttgt aaaaacggct tgggctttct tgtattacgt
      181 ctatttcttg cttatgaatt ttttgaagca gggttatcaa aactaaacgg tgaaaattgg
      241 tttgcctcaa ttcaagagag cttcccattc ccctttaacg tactaccagc cgatcttagc
      301 tgggctattg ctagcggagc ggaaatctta ctgcctattt tactcacttt gggtctgttc
      361 acccgcttca gtgcgttagg attaatgatc ttaacagcgg tagcgtggta tgctgttcac
      421 gctggcaatg gctacaatgt ctgcaacaat ggctataaaa tggcactcat ttatttagtg
      481 atgttagtac cacttttatt acaaggtgct ggctcgctct ctctcgattt tctgttacag
      541 aaaaaatccc caaccaaacg ctggttgaaa ttcctttaat cccttaattt atggagaatt
      601 tttaatgaaa aaagcaactt taatggcatt agctggtgca ttaactatgg caaccgcgac
      661 tgtaaacgct gaaactaaat cagtgaaagc agatgcttgt atggaagtaa aagatggcaa
      721 atgtgtaaaa accaaagcta cagaaggtaa atgtggcgaa ggcaaatgtg gagcaacctc
      781 ttctgagcca aaagcaaccg aaggtaaatg cggcgaaggg aaatgtggag caacggcacc
      841 aaaagcggca gaaggtaaat gcggtgaagg caagtgtgga gcttctaagt aagtagcgaa
      901 ggtgcgtttt ctaaacgcac ctaacctctt ataaacacaa tctacaagcg gttaaattcc
      961 gctgattttt tgcaaattaa aggaacaaga cgatgttaaa atttactggt gccggtctag
     1021 gctatcgccg tgacctagcg gacggatttt tatctctcac agataacaat ataattaatt
     1081 ttatggaagt agccccagaa aactggctga aaatgggcgg tcaggctcgg tataacttcg
     1141 ataaagtcgc tgaaaaaatc cctgttatta ttcacggttt atccctctct ctcggcgggc
     1201 aagcgccttt agatatgacg ctactaaaag ggatcaagaa tatgcagcag cagtataact
     1261 cctttttctt ctctgatcat ttaagctatt gcgaatgtga agggcattta tatgacctgc
     1321 tgccaatgcc gtttaccgat gaagccgtac atcacgtggc aaatcgcatt aaacaagtgc
     1381 aagattattt aggtatgcaa atttcccttg aaaatacgtc ctactatctg cattcaccaa
     1441 ccagtacgat gaatgaggta gagtttctca atgcaattgc ccaagaagcg gattgtggca
     1501 ttcaccttga tgtgaataat atttatgtta atggtgtcaa tcacggcttg cttgatccat
     1561 ttgtatttat tgatcaagtg gataaatctc gtgtcaatta tattcatatt gcaggccacg
     1621 atgaagatca cggtgcggcg gaagacgtta aaatgaccga gggtgaatca tttaatatgg
     1681 taaaaggcga actacgtcat ctgccaactt tactggtcga tacacacgga gaagctgtac
     1741 aaaaaactgt gtgggattta ctagagtaca cctatgaaca cctaccgcat attccaccaa
     1801 cactgttaga acgggatttc aacttcccac cgtttcaaga actgattagt gaagtacagc
     1861 acattatgag cttacagaaa aaatatgcaa aaacgaggct aaaaaatgcc gcttaaactc
     1921 gttgaagaac acaaaccgtt actcaaagag attcaagccg agtttgcgaa tgcaattcgc
     1981 cataacgatg taaagctgat gagtggtaat atctctccac agcggcttgg cgtctattct
     2041 cgcttggttc gcaataatac tctcggtttt atcgaccgtt gttatgtaca gctccctcaa
     2101 catctaagtc ctgaagaatg ggttgccgtt aaagaaaaat ttatacgaga aggtaaggct
     2161 cattcgccct ttttccaaga tattgctggc gaatttctca atttttgccg agaaaatggc
     2221 tgtatgccac cacacttgct tgagttgatg gactttgaaa atgctcaatt actggctgag
     2281 gtatcaatgg caaccgtacc atcagaattt gaatgggata acgatactat aatgcagttt
     2341 tctggtgcag ctgagctgcg tcaatatgcg gtaaacttta tccgtagcga gttcaaacag
     2401 tttgactttg agccgaccaa cgtactgatt tggcgagata cggaatttgg catctattac
     2461 aaccgcttgg aagagctgga tttcttcctg cttagctcgt tgcaagaggg ggcaaattcc
     2521 ctgaatggct tactagcaga actcagcgag tttaccgagc aaaatctgga gctaaaagcc
     2581 ttgttacgcc aactttggga aaaatgggtt gaagcagagg tgatttatcc tgtctatcct
     2641 gaatgaggat aaggtatgtg atatgtactg acatacatag taggggcggg
//
LOCUS       DANLZC010000214         2659 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.215, whole genome shotgun sequence.
ACCESSION   DANLZC010000214 DANLZC010000000
VERSION     DANLZC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.215"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            22..834
                     /locus_tag="PXE44_002483"
     CDS             22..834
                     /locus_tag="PXE44_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5589938.1"
                     /translation="MGRPELERAVLSVQNQTYPCKHYVFVDGEKYHQQAADILKNFPS
                     VIVTYLPMNTGADGWVNSSINAIAPFLVKEDIICYLDDDNWFEPTHVESGVHALLEMQ
                     SDYAYSLRNFYDLDGEFLCIDTIESLGEYNCVYQEPLECNYQLGGVKGVLSINLNDCQ
                     HIDTNCYFIRSEIARLISTAWYTGIHNDRNVYKKLQELALKGNCTKKVTVNYTLDTRK
                     YYGSVFNELLEEPFLLSEYDAYQFTNEVIKFHSEESLKAWGGCLIWEQNNGS"
     gene            966..1664
                     /gene="aphA"
                     /locus_tag="PXE44_002484"
     CDS             966..1664
                     /gene="aphA"
                     /locus_tag="PXE44_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDL5589939.1"
                     /translation="MTAATMFASLIAFSAQAGGPKVAYTQPGIDMRTHEAQQAPIHWV
                     SIEQIKESIKDLPPMAVSFDIDDTVAATSGCFHYGKQKYSPDDFSYLKNQDFWDEINA
                     GCDKYSLPKQVAINLIKMHQERGDQVYLITGRTAGKNEQVTTIMQKYLGIKNMQPVNF
                     MGGKAHSTKYDKTPAIIEHKVQIHYGDSDDDILAAREAGIRGIRILRAANSNYTPFPQ
                     AGGYGEEVVVNSSY"
     gene            1845..2606
                     /gene="udp"
                     /locus_tag="PXE44_002485"
     CDS             1845..2606
                     /gene="udp"
                     /locus_tag="PXE44_002485"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDL5589940.1"
                     /translation="MSEVFHLGLTKAMLKGAKIAIVPGDPARSERIAKKMDNPEFLAS
                     TREFTSWLGYLDGEPVVVCSTGIGGPSVSICVEELAQLGVRTFLRIGTTGAIQEHINV
                     GDILITTGAVRLDGASQHFAPMEYPAVANFECTNALYAAAVESGVTPYVGITASSDTF
                     YPGQERYDTFTGKIYRHFQGSLKQWQDLNVMNFEMESATLFTMCSALGLRAGMVAGVI
                     VNRTQQEIPNEATIHDTEHKAVSVVVEAARKLIKG"
BASE COUNT          848 a          431 c          557 g          823 t
ORIGIN      
        1 gtagctgtta tcagttcaac aattggtcgt cctgaattag aaagagctgt tttaagcgta
       61 caaaatcaga cttatccttg taaacattat gtttttgttg atggagagaa atatcaccaa
      121 caagcggctg atattttaaa gaattttcca agtgttattg ttacttatct cccaatgaat
      181 accggggctg atggttgggt aaatagcagc attaatgcaa ttgcaccatt tttagtgaaa
      241 gaagatatta tttgttatct ggatgatgat aattggtttg aacctactca cgttgaatct
      301 ggtgtgcatg ctttattaga aatgcaatct gattatgctt actctttacg taatttttat
      361 gatttagatg gcgagttttt gtgtattgat acgattgaat cgttgggaga atataattgc
      421 gtttatcaag aaccattaga atgtaattat caattaggtg gtgttaaagg tgttttatca
      481 ataaatctga atgattgtca gcatattgat acgaattgct attttattcg tagtgagata
      541 gctcgcctta tcagtacggc ttggtacaca ggtattcata atgatagaaa tgtatataaa
      601 aaattacaag aattggcact aaaaggaaat tgtacaaaga aagttacggt taattatact
      661 cttgatacac gtaaatatta tggatcagta tttaatgaat tattagagga gccattttta
      721 ttaagcgaat atgatgccta tcaatttacg aatgaggtga ttaaatttca ttcagaagaa
      781 agccttaaag cttggggagg ttgcttgatt tgggaacaga acaatggctc ataagcgtta
      841 agcgtaatcg tttgcttgtg atcatattca caaaataagg tttttgtatt agctaagagt
      901 gcctaataag attaaaatag gtatggttaa tcatatggag gtattttata aaatttacat
      961 taaaaatgac ggcagcgaca atgtttgcat ctcttattgc tttttcagca caagcaggcg
     1021 gaccaaaagt agcttatacc cagccgggaa ttgatatgcg aacacatgaa gcacagcaag
     1081 cgccaattca ttgggtatct attgagcaaa ttaaagaaag catcaaagat ttacccccta
     1141 tggcggtaag ttttgatatt gatgatactg ttgctgcaac tagcggttgc ttccactacg
     1201 gaaagcaaaa atattcgcca gacgatttca gctacttaaa aaatcaagat ttttgggacg
     1261 aaattaatgc tggctgtgat aaatattctt tgccaaagca agttgctatt aatttaataa
     1321 aaatgcacca agaacgtggt gatcaagttt atttaattac ggggcgtacc gcaggtaaaa
     1381 atgaacaagt tacaacgatt atgcaaaagt atcttggtat taaaaatatg caaccggtaa
     1441 attttatggg ggggaaagca cattcaacaa aatatgataa aacccctgcg attattgaac
     1501 ataaggtgca aattcactat ggtgacagtg atgatgatat cttagctgcc cgtgaagccg
     1561 gtattcgagg tattcgtata cttcgtgcag caaattctaa ttatacgcca ttccctcagg
     1621 ccggaggata tggtgaagaa gttgttgtca attcaagtta ttaatttctt agtctgaaat
     1681 atggccacca ttgcgtggcc atcatttttt atttatataa tgacgaaacc gtttaccttt
     1741 gatctagttc acaaaatcaa actttgcttg caaaaaattg tcgtaaatta accgcttgct
     1801 aagatagaat ggctcatatc cagttagata aaaaggagat aaaaatgtct gaagtatttc
     1861 atttaggttt aaccaaagcc atgctcaaag gtgcaaaaat tgcaatcgtt ccgggcgatc
     1921 cggctagaag tgaacgtatt gccaaaaaaa tggacaaccc agagttttta gcctcaaccc
     1981 gtgaatttac ctcttggtta ggttatttag atggcgaacc ggtggtggtt tgttcaaccg
     2041 gtattggcgg accgtcggtg tcgatttgtg tggaagagtt agcacagtta ggtgttcgca
     2101 cattcttacg cattggcaca acaggtgcaa ttcaagagca tattaatgta ggcgatattt
     2161 taattaccac cggagcggtg cgtttagatg gtgcgagcca acattttgca ccaatggaat
     2221 atcctgcagt agcgaatttt gaatgtacta atgctcttta tgcagcagca gtggaatcag
     2281 gcgttacacc ttatgtgggg attacagctt catcagacac tttctacccg gggcaagaac
     2341 gttacgacac tttcaccggc aagatctatc gtcatttcca aggttcatta aaacagtggc
     2401 aagatctcaa tgttatgaat tttgaaatgg aatccgccac tctctttacg atgtgttcgg
     2461 cattaggctt gcgagcaggt atggttgctg gtgtaattgt aaaccgcact caacaagaga
     2521 ttccgaatga agcaaccatt cacgacaccg aacataaagc cgtttcggta gtagtggaag
     2581 cggcaagaaa attaattaaa ggctaatctt taaatttaaa agcaaaaaag gtaaattgtc
     2641 tttgttacaa tttaccttt
//
LOCUS       DANLZC010000215         2515 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.216, whole genome shotgun sequence.
ACCESSION   DANLZC010000215 DANLZC010000000
VERSION     DANLZC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.216"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            295..2268
                     /locus_tag="PXE44_002486"
     CDS             295..2268
                     /locus_tag="PXE44_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5589941.1"
                     /translation="MHSILKKILGKSKSSSQSSVKSDNVFAIEQDSEGWLYPKNASQL
                     LNTELRQKYLGLLWQQVSMNRQMFEVLYQQPIERYAEMVQLLPASESHHHSHIGGMLD
                     HGLEVIAFSAKVRQNYILPPNVAPEEQSKQRDAWTAAAIYLALVHDIGKVVVDIEITL
                     KDGSRWFAWNGQPPQPYKFKYIKGRDYHLHPVLGGFLANQLIPKSAFDWLGQYPEVIS
                     SLMYAMSGHNDKAGLLSEIVQKADQHSVTLAIGGDVNKLVQRPVNSLPKQIVLALRYL
                     LEQKIKLNTPKGPADGWFTEDGLWLVSKTAADNIRTYLLGQGISVPKDNPKLFDEMQS
                     LGIVEESPSGGAIWSCRIQADSGWTPATTFTLLRVKPEVAWENINDRPAYFAGKVKVI
                     EQITEEVNAEVQPPLVEETTKISLLPSKQSEAIIPSEETEISKAITSSDNATSAEVII
                     NNENDLTSFVLDMFAPTETIEQNISVISSNKEQQIVMTTLTEQEKVPPKKVKLEKSKK
                     KNLQLTTLEPWNDMINPITGKEFVNWLKEGIATNKFAINKPSAKLHIVDGNLFLVTPV
                     IFEMYLQECGQPYDKETINNLQYDFQGLGLHSLKKTMTNGKPDTINFWKCDVIGPRKT
                     SSLTGYLIKNTHLLFGDKMLLNNHCLTLQEEDT"
BASE COUNT          827 a          464 c          506 g          718 t
ORIGIN      
        1 tatcattcgt gtattgtaat ctcgcaattc aacatcacta ataaaaaatt ctccaacata
       61 ttcagagagt tttttctact gataccaact aaattttggt tcaatactcg aagttaaatt
      121 tggttcagag aaaaaagcct ctaaatggca aaagggtaag gggccctaac gggcaagggt
      181 aatcccctaa gggggataag agtaaccttt ccaaggggaa aggtcttacc caaaagggca
      241 aagggctaaa cggctttctt tcaacgcaaa catcatcttt ttcggtctat ttcaatgcac
      301 tcaattctca aaaaaattct tggcaaaagt aaatcctcgt ctcaaagttc ggttaaatct
      361 gacaatgttt tcgctattga gcaggatagt gagggctggt tatatccgaa aaatgcctcc
      421 caactactta atacggaatt acgccaaaaa tatctgggtc tactttggca acaggtatca
      481 atgaaccgtc aaatgtttga ggtactttat cagcagccaa tagaacgtta cgctgagatg
      541 gttcaactat taccagcttc tgaatcacat catcattcac atatcggcgg aatgctcgat
      601 cacggtttgg aagttattgc tttttcagcc aaagtccgtc aaaactatat attgccaccg
      661 aatgtcgcac ctgaagaaca atctaagcaa cgagacgctt ggactgctgc cgcaatttat
      721 cttgccttgg ttcatgatat tggcaaagtt gtggttgata ttgaaatcac tcttaaagac
      781 ggctctcgtt ggtttgcttg gaatggacag ccacctcaac cttataaatt caaatacatc
      841 aaaggtcgag actaccatct tcatccggtg ctaggaggat tccttgcaaa tcagctcatt
      901 cccaagtctg cgtttgattg gctgggacag tatccagaag tcatttcttc actgatgtat
      961 gcaatgtctg ggcataacga taaagccggg cttttatctg agatcgtgca gaaagccgat
     1021 cagcatagtg ttacgctagc aatcggtggc gatgtgaata agctggtaca gcgaccagtt
     1081 aattcattgc cgaagcaaat tgtgctggct ctacgttatt tactggagca gaaaatcaaa
     1141 ttaaatacgc caaaaggacc agcagacggc tggtttaccg aagatgggct gtggctggta
     1201 agtaaaactg ctgccgataa tatccgaact tatttactgg gacaggggat ttccgtgcct
     1261 aaagataatc caaaactgtt tgatgaaatg caatctcttg gtattgtgga agagtctcct
     1321 tccggcggtg caatttggag ctgccgtatc caagccgatt ccggttggac accggcgaca
     1381 acatttacgc tattacgagt taaaccggag gttgcttggg agaatatcaa cgatagaccg
     1441 gcgtattttg ccggaaaagt caaagtgatt gaacagataa ctgaagaggt caatgcagag
     1501 gttcagcctc cattagttga agaaaccact aaaatttctc tattaccgag caagcaatcg
     1561 gaggctatta ttccgtctga ggaaacggaa atatcgaagg caattacaag ttcagacaat
     1621 gcaacatctg cggaagtaat cataaataat gaaaatgatt taacttcttt tgtattggat
     1681 atgtttgctc ctacagagac aattgagcaa aatatttctg tcatttcttc aaataaagag
     1741 cagcaaatcg taatgacaac acttacggaa caagaaaaag ttccgccgaa aaaagttaaa
     1801 ttagagaaaa gtaagaaaaa aaatcttcaa ttaaccactc tagaaccatg gaatgatatg
     1861 attaatccaa ttactggaaa agaattcgta aattggttga aggaggggat agcaaccaat
     1921 aaatttgcaa ttaacaaacc ttctgcaaaa ttacatatag tagatggtaa tttattttta
     1981 gtcacaccag ttatttttga aatgtatttg caagaatgtg gccaacctta tgataaggaa
     2041 actataaaca atcttcagta tgatttccag gggcttggtc ttcattctct taaaaaaacc
     2101 atgactaatg gtaagccaga tacaataaat ttttggaagt gtgacgtgat tggtcctaga
     2161 aagacttcct ctttaacagg atatttgata aaaaatactc atctcttatt tggggataaa
     2221 atgttactaa ataaccactg tttaacttta caggaagagg acacataata tggattctaa
     2281 gatttccttc gatatattat tggaacgatt tttttctatc gtcattattt acgggcggac
     2341 acgaaacgaa gttataacaa tattattcga attatcaaaa agcgttatcc aaacttaaat
     2401 gcgaatgatg tgactactga tatgttaatt gaatggcgta atcaagttgt tggtaataca
     2461 attaaagctg tgacgtggaa taattatgtg agacacctaa aagcattata taatt
//
LOCUS       DANLZC010000216         2490 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.217, whole genome shotgun sequence.
ACCESSION   DANLZC010000216 DANLZC010000000
VERSION     DANLZC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.217"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            28..1095
                     /gene="hisC"
                     /locus_tag="PXE44_002487"
     CDS             28..1095
                     /gene="hisC"
                     /locus_tag="PXE44_002487"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL5589942.1"
                     /translation="MTISQLSRKNIQALTPYQSARRLGGSGDVWLNANEYALSPNFDL
                     TDRTFNRYPEPQPQAVIEGYARYAGVAPENVLVSRGGDESIELIIRAFCEANDSILYC
                     PPTYGMYAVSADTCGIATKTVPLTADFQLDLAQIKANLEGVKVVFVCSPNNPTGNLIK
                     RSDLFELLQITAGKAIVVVDEAYIEFCPEARLVSELANFPHLAIIRTLSKAFALAGLR
                     CGFTLANAELIGVLQKVIAPYPLPVPVSDIAAQALSPQGIEQMKTRVAEVITLRAELQ
                     KNLENLPLVEQVFDSEANYLLFKCQDGQKVFKALWEQGIILRDQHKALGLQNCIRITV
                     GAREENLRVVEAITALSQFQR"
     gene            1280..2368
                     /gene="hisB"
                     /locus_tag="PXE44_002488"
     CDS             1280..2368
                     /gene="hisB"
                     /locus_tag="PXE44_002488"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDL5589943.1"
                     /translation="MQPTLFIDRDGTLIDEPKTDFQIDSLEKLKFEKNVIPALLKLKG
                     KYRFVMVTNQDGLGTSSFPQADFDKPHNAMMALFNSQGIEFDDVLICPHKPEDGCDCR
                     KPHTKLLQKYIDRQLFDPANSFVIGDRATDVQLAENLGIRALQYHPEKLNWDLIAEKL
                     LGEAVTNIGERPKRFAEVIRKTKETDIKVQVWLDETGNNEISTGVGFFDHMLDQIATH
                     GGFRMNITTKGDLWIDEHHTVEDTALALGTALKQAIGDKRGIARFGFVLPMDESRAEC
                     AMDLSGRPFIKFKADFKRDKVGDFSTELTEHFFQSIAFTMLATLHIKAKGDNDHHKIE
                     SLFKVFGRTLRQCIRIEGNELPSSKGVL"
BASE COUNT          701 a          618 c          602 g          569 t
ORIGIN      
        1 tatgtataaa ttatacagga acacaatatg acaatttcac aactttcccg taaaaacatt
       61 caagctctca ccccttacca atcggcacgg cgtttggggg gtagtggcga tgtgtggctg
      121 aatgccaatg aatatgccct ttcgccaaac tttgatttaa ccgaccgcac ttttaaccgc
      181 taccctgaac ctcaaccgca ggcggtgatt gaaggttacg cccgatatgc aggcgttgca
      241 cctgaaaacg tgctggtttc ccgtgggggc gatgagagca ttgagctgat tatccgagcc
      301 ttttgcgagg caaacgacag cattctctac tgcccaccaa cctacggaat gtacgccgtg
      361 agtgccgaca cctgtggcat tgcgaccaaa actgtgcctt taaccgccga tttccagttg
      421 gatttagccc aaatcaaagc taacctcgag ggcgtgaaag tggtgtttgt gtgcagcccg
      481 aacaacccga caggcaactt aatcaagcgg tcggatttgt tcgaactttt gcaaatcacc
      541 gcaggcaaag cgattgtggt ggtggacgaa gcctacatcg aattttgccc tgaagctcgc
      601 ttggtaagcg agctggcgaa tttcccacat ttggcgatta tccgcaccct atccaaagcc
      661 tttgccttgg cgggtttgcg ttgtggcttt accttggcaa acgctgagct gatcggcgtg
      721 ttacaaaaag tgattgcccc gtatccgctg cctgtgcctg tttccgacat cgccgcccaa
      781 gccctctccc cacaaggcat tgagcagatg aaaacccgtg tggcggaagt catcaccctg
      841 cgtgccgaat tgcaaaaaaa tctggaaaac ttaccgcttg tagaacaagt ttttgacagc
      901 gaagccaact acctcctatt caaatgccaa gacggacaaa aagtcttcaa agcactttgg
      961 gagcaaggca ttattttgcg tgaccaacac aaagcgttag gattgcaaaa ttgcattcgg
     1021 attacggtgg gggcgagaga ggagaatttg agggtggttg aggcgattac agcactgagc
     1081 caatttcaga gatagaattt aacgagcagt attttaagag acatttatag cctagcacgg
     1141 catagccgta ctaggttacg cataagtcag tccctttggg actgaataca gagaggcaaa
     1201 gtctctcact aatgcttaac cccatatgcc ttggcgtgtg gggagagtaa tgaataagat
     1261 acagatacag gagaaaccaa tgcaacccac cctttttatc gaccgtgacg gcacgttaat
     1321 tgatgagcca aaaaccgatt ttcaaatcga cagcttggaa aagctgaaat tcgagaaaaa
     1381 cgtgattcct gccctgctca aactcaaggg caaataccgc tttgtgatgg ttacgaacca
     1441 agacggctta ggcacgagtt cgttcccaca ggcagatttc gacaaaccgc acaatgcaat
     1501 gatggcgttg ttcaactcgc aagggattga atttgatgat gtgctgattt gtccacacaa
     1561 gcccgaagac ggttgcgatt gccgcaaacc gcacaccaaa ctgctacaaa aatatatcga
     1621 ccgccaactg ttcgacccag ccaacagctt tgtgattggc gaccgtgcta ccgatgtgca
     1681 actggcggaa aaccttggca ttcgggcgtt gcaatatcac cctgaaaaac tgaactggga
     1741 tttgattgct gaaaaactac tgggcgaagc cgtcaccaac attggcgagc gaccaaagcg
     1801 ctttgccgaa gttatccgta aaaccaaaga gaccgacatc aaagtgcaag tgtggctgga
     1861 cgaaacaggc aacaacgaaa tcagcaccgg cgtgggcttt ttcgaccata tgcttgacca
     1921 aattgccacc cacggcggtt ttagaatgaa catcaccacc aaaggcgact tgtggattga
     1981 cgaacatcac acggtggaag acaccgccct cgccctcggc accgcattaa aacaagcgat
     2041 tggcgacaaa cgcggcatcg cccgtttcgg cttcgtgttg ccgatggacg aaagccgtgc
     2101 ggaatgtgcg atggacttat caggcagacc tttcatcaag ttcaaagcgg atttcaaacg
     2161 cgacaaagtg ggcgatttca gcaccgagct caccgaacac ttcttccaat ccatcgcctt
     2221 cacgatgctc gccaccctgc acatcaaggc aaaaggcgac aacgaccacc acaaaatcga
     2281 aagcctgttc aaagtcttcg gcagaacact cagacagtgc attcgtattg aagggaatga
     2341 actaccaagc agtaaaggtg tattataatc accgtagggg cgggttttaa cccgcccgaa
     2401 agtgcggttg aaatttgcaa agtttttggg gattttgacc gcttgttata aaggatatat
     2461 tgatgattag agaagttatt gactttataa
//
LOCUS       DANLZC010000217         2474 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.218, whole genome shotgun sequence.
ACCESSION   DANLZC010000217 DANLZC010000000
VERSION     DANLZC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.218"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            32..751
                     /locus_tag="PXE44_002489"
                     /pseudo
     CDS             32..751
                     /locus_tag="PXE44_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            811..1260
                     /locus_tag="PXE44_002490"
     CDS             811..1260
                     /locus_tag="PXE44_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HDL5589944.1"
                     /translation="MLAKLKWNQLACVTLAFCSASVFSAHHEIKMLDNNAEGSMVFEP
                     GYVKAEVGDTVTFIPSHKGHWVESRSIPEGAEKFLSKEDEEFTITLTHEGVYVYYCPP
                     HRTMNMAGIIQVGQALNLEATQKEVEKIEKRTKENKGRLFEYLEQVK"
     gene            1361..1882
                     /locus_tag="PXE44_002491"
     CDS             1361..1882
                     /locus_tag="PXE44_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HDL5589945.1"
                     /translation="MYKAITLIEILTTITIMVIAVYFISPVIFTLQDRIALHSEIENI
                     QSFIQQIQTKARYTKRNYTLTLSQRNNQWCMIAVIKPINTSKQIICDCLNKHFCDLGK
                     EYHLYTPTHQQIVLKNKSLYPKSFINVDGVSGQLESKCIKLSLNKENEILQFEQLGRV
                     YVAAKNKRSTCKD"
     gene            1914..>2474
                     /locus_tag="PXE44_002492"
     CDS             1914..>2474
                     /locus_tag="PXE44_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589946.1"
                     /translation="MASALGAFLLLLVASSYADFYYTQTKQKEQLYLQAEAHQLLNYF
                     QQHFQHIGYQGSHRTDSNFDLFQQAGKSLNIVHKNCIIGFYDLNQDGCLGKRKTKTAA
                     CKLGTVNNTREVLKEIVGFKVENNEIYTFSSQLDNCVKEQCQTLLENCDGNWSKFTEA
                     NNFKTKKLDFSWRQEDNLLKIELEIES"
BASE COUNT          856 a          403 c          491 g          724 t
ORIGIN      
        1 gttttagtcg ttatctagga cagcccctac ttttaatact cgtaagcccc cttttgataa
       61 ccctaaagtt cgtcaagcat tgaattatgc ggtgaataaa actgaaattt taacttccgt
      121 ttaccaaggc tctgcaacgc cagctaaaaa cccaatgcct tctacgattt ggggatataa
      181 caacgaagta gccggttatg aatttaatcc ggaaaaggcg aagagcttgc ttaaagaggc
      241 cggattagaa aatggctttg aaacagaaat ttgggcaatg cctgtttctc gcccttacaa
      301 tccaaatgcc cgtagaatgg ctgaaatgat tcaagaggat tggaaagcaa taggggtaga
      361 ggcaaagata gtgagctatg aatggggaga atatcttaaa cgaatgggaa acggcgagca
      421 cgctaccgga atgattggtt ggaccggaga taacggcgat ccagataatt tcctcaatgc
      481 cctactcagt tgtaatgcgg tagaacaagg ctcaaactat gcagggtttt gtaatccaga
      541 gtttgaccgt ttaattaatg aggctgcgac tatcagtgat caaaacaagc ggtcagattt
      601 atataaaaaa gcgcaattca tctttaatga gcaagcacct tggttaccga ttgcacattc
      661 aacggtgtat tttccggtaa ggaaagaggt aaaaggctat gttgtcagcc cgtttattgc
      721 tcataatttt tatggtgttg agctggaata acctaaggta taatgaattg aactcatttt
      781 gtgcctcata aatttaggag caataaatat atgttagcga aacttaagtg gaatcaatta
      841 gcttgtgtaa cattggcatt ttgttcagcc tcagtatttt cagcccatca cgaaatcaaa
      901 atgttagata acaatgcgga aggatcaatg gtatttgagc ccggttatgt aaaagcagag
      961 gtgggcgata cggtaacatt tattccgtct cataaagggc attgggttga aagccgctcg
     1021 attccggaag gtgcggagaa gttcttatct aaagaagatg aggaatttac tattaccctt
     1081 actcacgaag gtgtttatgt ttactactgt ccgccacacc gaaccatgaa tatggccgga
     1141 attatccaag taggacaagc tctcaatctt gaagccacac aaaaagaagt ggaaaaaatt
     1201 gaaaaacgca cgaaagaaaa taaaggacgt ttatttgaat atttagagca agttaagtag
     1261 aagtgattta attttgcgat ctgcatcgaa aaatcaatat ttaatagttt acaaatgatg
     1321 cttaaattct aagataccct ctatcaatga gggtattttt atgtataaag ccatcacatt
     1381 aattgaaata ctaaccacta tcactattat ggtgatcgct gtttatttta tctcgccggt
     1441 tatttttaca ctgcaagatc gaattgcact tcatagtgaa attgaaaata tccaatcttt
     1501 tattcagcaa atccaaacta aagcacgtta taccaaacgt aattatacct taaccctttc
     1561 tcagcggaat aatcagtggt gtatgattgc ggtaattaag ccaatcaata ctagcaaaca
     1621 aattatttgt gattgtttga ataaacattt ttgtgattta ggaaaagaat atcatcttta
     1681 tacgccgact catcagcaga ttgtattaaa aaataaaagt ttatatccga aatcctttat
     1741 taatgttgat ggggtaagtg gtcaattaga atcaaaatgt attaagctca gtttaaataa
     1801 agaaaatgaa attttgcaat ttgaacaatt aggacgggtt tatgtggcag caaaaaataa
     1861 acgtagcacc tgcaaagatt agtttatctg cttttagcct aattgaagta ttaatggcta
     1921 gtgcattagg ggcgttttta ttattgcttg ttgcttcaag ttatgccgat ttctattaca
     1981 cacaaaccaa acaaaaagaa cagctctact tgcaagcaga agctcaccag ttactaaact
     2041 acttccaaca gcattttcaa cacattggct atcaaggctc tcacagaaca gacagtaatt
     2101 ttgatttatt tcagcaagca gggaaaagcc tgaatattgt gcataaaaat tgcattattg
     2161 gattttacga tttaaaccaa gatggctgtt tgggaaaacg taagacaaaa acggccgctt
     2221 gtaagttagg aacagtgaat aataccagag aggtgttaaa agaaattgtt ggctttaaag
     2281 tagaaaataa tgaaatttat actttttcat cgcaattaga taattgtgtg aaagaacaat
     2341 gccaaacatt attggaaaat tgcgatggaa attggagtaa atttaccgaa gcaaataatt
     2401 tcaaaactaa gaaactggat tttagttgga gacaggaaga taacttatta aaaattgagt
     2461 tagaaataga atca
//
LOCUS       DANLZC010000218         2468 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.219, whole genome shotgun sequence.
ACCESSION   DANLZC010000218 DANLZC010000000
VERSION     DANLZC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.219"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            459..837
                     /gene="rnpB"
                     /locus_tag="PXE44_002493"
     ncRNA           459..837
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PXE44_002493"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            898..1536
                     /locus_tag="PXE44_002494"
     CDS             898..1536
                     /locus_tag="PXE44_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HDL5589947.1"
                     /translation="MKFIKTIITLIALYSMPVFSHPHSFVDLKTNVIVEGSMLKSFQM
                     EWMLDEIASSELIYEVKNSQDKEKTQQNITAEMVQTALQNHYFSTLYDSQNQPIKFTT
                     KPTATRFEIKNNRVIFYITFHLAKPHELKQSKVRFYTYEPSYYIAMEYNRPADVNTSN
                     ASCKATLVQPQVDSKLRLYASGLDKNQSLDMPENGDYSLGAQFAQKVEIICD"
     gene            1527..>2468
                     /locus_tag="PXE44_002495"
     CDS             1527..>2468
                     /locus_tag="PXE44_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HDL5589948.1"
                     /translation="MRLKATYWGMIVIGFLSLFALYQLYPMILFKVMEWQKNFNLQLS
                     ASLNELAENQSKAGITLVFISFLYGVFHAVGPGHGKFILSSYLSFEKTKLNQAMKITF
                     ASALVQGLVAISLVTVIVVIFTLSRQYFNLTLKWVERGSFGLMILFGIYWCYQVYRTV
                     NRARKPIIKSIRISQKKAGKLPLVTQKGHIHTENCSCRHKHLPTSNEMQQVRDWKAQL
                     MLVLSIGSRPCSGAILVLFLSYTLNLYAWGVISALAMAVGTGLTLSLFAYLVIIARNK
                     AVKVSQWYFSAQINRNIVWILKFTLGLALILFGMVLFH"
BASE COUNT          771 a          395 c          537 g          765 t
ORIGIN      
        1 attaattcgt gctgtcatca acacgatcgt gggcgtaaac ggaaaagtca gtagagcgga
       61 agcagataag cggttaaggg attgtttgat tgcgcaaaat agacctattt ttgcgtggag
      121 cacttggttg gcggtaagat gttttggatg gtatttttgg aatgagaaaa aaggaaaata
      181 gcttgtttta taatctgatt aaaatttgag tttcggaaaa atgtatatag tttcggaaat
      241 agaaaggaaa ttgattaaat aaccctttga aattattgta ataaaataga gaaaccaaaa
      301 ttagttcact tagacttcaa acgtgcgtaa tttttacgag tgaattaaag cgaagaggat
      361 taagttttta aatgaactta atcctttttt attttgccat aataactaca agcggttatt
      421 tccttgcaat tttttgcgaa agcattatac tctagtgctg agttggttag gcaatcgctg
      481 gtttattgaa gcctctaaat tcgtgaaaac gtaaactggg ggacaagtaa acgagaggaa
      541 agtccgagct acacagggca gagtgccgga taacgtccgg ggggcgtgag cctacgacca
      601 gtgcaacaga gagcagaccg ccggctttgt cggtaagggt gaaagggtgc ggtaagagcg
      661 caccgtgtgg gtggtaacat tccacagcag ggtaaactcc actcgtagca agaccaaata
      721 ggaactcaat cggtggcccg ctgagagttc gggtaggttg cttgagcgta tgtgtaaatg
      781 tacgtctaga ggaatgattg tccacgacag aactcggctt atcgaccaac tcactaaatt
      841 cttgaaatgc gaacaggggg aacttgttcg cttttttatt ttatagtgaa agtaaatatg
      901 aaatttatta agacgattat cacgttaatt gcattatatt caatgccagt tttttctcac
      961 ccacattcat ttgttgattt gaaaacgaat gttattgtgg aaggctcaat gttgaaatct
     1021 ttccaaatgg aatggatgct agatgaaatt gcttcttctg agttaattta tgaagtgaaa
     1081 aatagccaag ataaagaaaa aactcagcag aatatcactg cagaaatggt gcaaaccgca
     1141 ttgcaaaatc attattttag caccttatat gatagtcaaa atcagccgat aaaatttacg
     1201 acaaagccca cagcgactcg ctttgaaatc aagaataaca gagtgatttt ttatattact
     1261 tttcatttag ctaaacctca cgaattaaag cagagtaaag tgagattcta tacttatgaa
     1321 ccgagttatt atattgcgat ggaatataat cgccctgctg atgtcaatac cagtaatgca
     1381 agttgtaaag caacattggt gcaacctcaa gtagatagta agttacgctt atatgcttcc
     1441 ggtttggata aaaaccaatc actggatatg cctgaaaatg gagattactc tcttggtgcc
     1501 caatttgctc aaaaagtgga gattatatgc gattaaaagc aacatattgg ggaatgatag
     1561 tgataggatt tttgagccta tttgctcttt atcagcttta tccaatgata ttgtttaaag
     1621 tgatggaatg gcaaaaaaac tttaatttac agctttccgc ttcgctcaat gaattagctg
     1681 aaaatcaaag taaagccggt attaccttag tatttattag ttttttatat ggagtatttc
     1741 acgcagttgg gcctggtcac ggaaaattta ttctgagcag ttatctttct tttgaaaaaa
     1801 caaaattaaa tcaggcgatg aaaatcactt ttgcctcagc attggtgcag ggcttagtgg
     1861 caatttcatt ggtgacggtg attgtggtga tttttactct ctctcgccaa tattttaatt
     1921 taaccctaaa atgggttgag cgggggagct ttggtttgat gatcttgttc ggcatttact
     1981 ggtgttacca ggtgtatcga acggtgaaca gagcgcgaaa gcctatcatt aaatccattc
     2041 gtatttcgca aaaaaaagct ggaaaattac cgcttgtcac gcagaaaggg catattcata
     2101 ccgaaaattg tagctgtagg cataaacatt taccgacttc aaatgaaatg caacaagtga
     2161 gagattggaa agcacagctg atgttggtat taagtattgg ctcaagacct tgctctggcg
     2221 ctattcttgt tttatttctt tcttatacgc tcaatctcta tgcttggggg gttatttcag
     2281 cattagctat ggcagtagga acagggctga ctttaagctt atttgcttat cttgttatca
     2341 ttgcgagaaa taaggcagtt aaggttagcc aatggtattt ttcagctcaa attaatcgaa
     2401 atatcgtttg gatattaaaa tttacacttg gactcgcatt aattctattt ggaatggtat
     2461 tatttcac
//
LOCUS       DANLZC010000219         2437 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.221, whole genome shotgun sequence.
ACCESSION   DANLZC010000219 DANLZC010000000
VERSION     DANLZC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.221"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            107..1702
                     /locus_tag="PXE44_002496"
     CDS             107..1702
                     /locus_tag="PXE44_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDL5589949.1"
                     /translation="MQRHEQLAQVQQTQKWDFIIIGGGASGLGIAVDAASRGYKTLLL
                     ESHDFAKGTSSRSTKLVHGGVRYLANGDVALVREALRERGRLAKNAAHLFKNQSFIIP
                     NYKFKDCVMYRVGLGLYDMLSGNLSLGKTENISKTEAKRRLPTLNDTGLRTGTVYQDG
                     QFDDSRLAVNMAQTVVEHGGTVLNHAKVTSLHKTPQGKIDGVTFYDELNQQEHTVFGT
                     AVINATGVFMNDILSMDHGSTKKFVVPSQGIHLVLDKSFLPSNDALMIPKTSDGRVLF
                     AVPWHEVLVVGTTDTLIENPSYEPIPLEQEVNFILETAAQYLTKKPTRDDVKAVFAGQ
                     RPLAAPEKEGQSTKEVSRSHKVLTDATSGLITITGGKWTTYRQMAEDTVEEALKVHSE
                     LGNKPCVTVNLPIHGNIPAAQVNLQDHLYVYGSDIPQLRALMTETPEYAQKIHPRLDY
                     TVAEVIWAVRHEMAQTVEDVLARRVRLLFTDARAAIDSAAKVAAIMAKELGKDEMWQQ
                     QQVIQFLDVAKHYLLVDYQPQPI"
     gene            complement(1789..2088)
                     /locus_tag="PXE44_002497"
     CDS             complement(1789..2088)
                     /locus_tag="PXE44_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589950.1"
                     /translation="MENIKMEELKLTEKELLVLKGNALRMRFKAETNKTKNEIMQPFK
                     QVKQTGLALVNSPAVKTIALDFLVKRLLSMKGISFSVLGLTAFFLLQQKHKRDDS"
     gene            complement(2075..2431)
                     /locus_tag="PXE44_002498"
     CDS             complement(2075..2431)
                     /locus_tag="PXE44_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL5589951.1"
                     /translation="MTLLEIAQVRGEMARIEINEQKNQLISVLIFILLAFVFLLVSFV
                     SLLFGLDNYLLPEQKVKVFFSIAIIMFIAVTICGMMILSALKKQRHFMQSTLTELKLD
                     IAAFKSALTLKQNGEH"
BASE COUNT          805 a          453 c          468 g          711 t
ORIGIN      
        1 tgacatagat ctaattttta aacaaatgtc tttgaagtca ttttattttc gtttatgcta
       61 attaagtaat gattaaatgt tttaattcga gcaaaaggag caaaaaatgc aaagacatga
      121 acagctagct caagtacaac aaacgcaaaa atgggatttt attattatcg gtggtggcgc
      181 aagtggtttg gggattgcgg tagatgcagc ttcccgtggc tataaaacct tattattaga
      241 aagccatgac tttgcgaaag gtacatcgag ccgaagcaca aaattagtcc acggtggcgt
      301 tcgctatctt gcaaatggcg atgttgcact cgtgagagaa gcattgcgtg aacgaggtcg
      361 tttagctaaa aatgcagctc atttatttaa aaaccaaagc tttattattc caaactacaa
      421 attcaaagac tgtgtaatgt atcgtgttgg tttaggctta tacgatatgc tttcaggcaa
      481 cctgagttta ggaaagactg aaaacatcag taaaactgaa gcgaaaagac gtttaccaac
      541 cttaaatgat actggtttac gtacaggcac tgtttatcaa gatgggcaat ttgatgactc
      601 acgtcttgca gtgaatatgg cacaaacggt agtagagcac ggcggtacag tactaaatca
      661 cgccaaagtg acttcactgc acaaaacgcc acaaggcaaa attgatgggg tgactttcta
      721 cgatgaactc aatcaacaag aacatactgt tttcggcact gcggtgatta acgcaaccgg
      781 cgtatttatg aatgatattt taagtatgga tcacggtagc acgaaaaaat ttgtcgtacc
      841 aagccaaggg attcacttag tattagataa atcattctta ccaagtaatg atgcgttaat
      901 gattccaaaa acctctgacg gacgagtgtt atttgctgta ccttggcacg aagtgttagt
      961 cgtagggaca acagatactc tgattgaaaa tccttcttat gagccaattc cattggaaca
     1021 agaggtgaac tttattttag agacagccgc acaatatctg acaaaaaaac caactcgtga
     1081 tgatgtaaaa gcagtctttg ccggtcaacg tccgcttgcg gcaccggaaa aagaaggtca
     1141 aagcactaaa gaagtatctc gtagtcacaa agttctcact gatgcaacca gcggtttgat
     1201 tacgattaca ggtggtaaat ggacaactta tcgtcaaatg gcagaagata ccgtagaaga
     1261 agcgttaaaa gtccactctg aactaggcaa taaaccttgt gtgaccgtca acttaccgat
     1321 tcacggtaat attccggcag cacaagtgaa tctacaagat catctatatg tatatggctc
     1381 agatattcca cagttacgcg ccttaatgac tgagaccccg gaatatgcac aaaaaatcca
     1441 tccgagacta gattataccg ttgcagaagt gatctgggca gttcgccacg aaatggcaca
     1501 aacggttgag gatgtattag ctcgccgtgt tcgtttgctc ttcactgatg cgagagcggc
     1561 gattgatagt gcagcaaaag tggctgctat tatggcgaaa gaattaggta aagatgaaat
     1621 gtggcaacaa cagcaagtta tccagttcct tgatgttgcg aagcactatt tattggtaga
     1681 ttatcagcca cagccaatct aatacgctaa aagcaagcgg ttaaaatcga taaaaatatt
     1741 gcaattacgt tatttgcaac tttttaaacc attttaaccg cttgtatatt agctatcatc
     1801 tcttttatgt ttctgttgta ataaaaagaa tgctgttaac cccaatacag aaaagctaat
     1861 tcctttcata ctcaataatc tttttaccaa aaaatctaat gcaatggttt taactgccgg
     1921 tgaattgacc agtgcaagcc ctgtttgttt cacttgttta aaaggctgca taatctcatt
     1981 tttggtttta ttggtttccg ctttaaatcg cattctaagt gcattacctt ttagaactag
     2041 aagttccttt tcggttaatt taagctcttc cattttaatg ttctccattc tgtttcaaag
     2101 ttaaagcact cttaaatgcg gcaatatcta attttagctc ggttaatgta ctttgcataa
     2161 aatggcgttg ttttttcaag gcagaaagta tcatcattcc gcaaatagtc acagcaataa
     2221 acataataat ggcaatagaa aagaatactt taactttctg ttcgggtaac aaataattat
     2281 ctaaaccaaa taataaactc acaaaagaaa ctaataaaaa tacaaaagcc aataagataa
     2341 atataagtac tgaaattaat tgattttttt gctcattaat ttcaattctt gccatctcac
     2401 cacgcacttg agctatttcc aataatgtaa tgagact
//
LOCUS       DANLZC010000220         2398 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.222, whole genome shotgun sequence.
ACCESSION   DANLZC010000220 DANLZC010000000
VERSION     DANLZC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.222"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..1948
                     /locus_tag="PXE44_002499"
     CDS             <1..1948
                     /locus_tag="PXE44_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDL5589952.1"
                     /translation="LTQFSPAYIKKLEESIFLKSGKNGDIPHIQCLVRKKDIKLTPEE
                     AVRQLYLMNLHYEYDYPFERMELEYSVVFGREKKRADIVIFDRDNPNAVYIMVELKKP
                     KLKDGKEQLKSYCNATGAPIGVWTNGESISYYNRRDPNYFKDIPDIPNAQQKLSDLLQ
                     ERWTIQDLIDNDKIAKQRKSLKDLVIEMEDEVLANAGVDVFEELFQLIFTKLYDEMES
                     TRNKSRYLEFRNYGDTESELKAKIQSLFDRAKNKWEGVFAESAKINLSPSHLAICVSS
                     LEEVKLFNSNLDVVDEAFEYLINKSSKGEKGQYFTPRYVIDMCVKMLDPKPEETVIDT
                     AAGSCGFPVHSIFHVWEKQLKARGLERSHLFTAEEKLPEQTDYVKEKVFAIDFDEKAV
                     RVARTLNLIAGDGQTNVLHLNTLDYERWEDFTKEEEWNDVYGEGWKKLRKLRKTKNEN
                     RDFQFDVLMANPPFAGDIKETRILARYELGKNSKGKQQSKVGRDILFIERNLDFLKDG
                     GRMAIVLPQGRFNNSSDKYIRDFIAERCRILAVVGLHGNVFKPHTGTKTSVLFVQKWH
                     DELCPKVEDYPIFFATMQKSSKDNSGDKIYVKNADGTLKLDNHQHLIVEHDLFNHDNS
                     TQDGIAEAFIEFAKKEKLSFVGKD"
     gene            2047..>2398
                     /locus_tag="PXE44_002500"
     CDS             2047..>2398
                     /locus_tag="PXE44_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5589953.1"
                     /translation="MDSTFFLKKYLNHPSFNFPLSLNEVSLIKSGTTPTDRDDNLKEG
                     VVLLKTNDIRNNLLNKYSSVDYFISEDINEKMISSQLKEGDVLVNIVGATLEVVGRVA
                     YVSSTFPKANITQAM"
BASE COUNT          832 a          372 c          480 g          714 t
ORIGIN      
        1 attaactcaa ttttctcctg cgtatataaa aaaattagag gaatctattt tcctcaaatc
       61 agggaaaaat ggagatattc ctcatattca atgtcttgtt cgtaaaaagg atattaagct
      121 cacacctgaa gaagcagtac gccaactcta tttaatgaat ttgcattatg aatatgatta
      181 tccatttgaa cgaatggaat tagaatattc tgttgtattc gggcgtgaga agaaacgagc
      241 ggatattgtc atctttgata gagataatcc taacgctgtc tatattatgg tggagctgaa
      301 aaagccgaaa ttaaaagatg gtaaagaaca attaaaatct tattgtaatg cgactggagc
      361 acctattggt gtatggacaa atggcgaaag tatttcttac tataatcgcc gtgatcctaa
      421 ttattttaaa gatattcccg atattccgaa tgctcaacaa aaactttctg atttattaca
      481 agaacgttgg actattcaag atctgataga taatgacaaa attgccaaac aacgtaaatc
      541 attgaaagat cttgttattg aaatggaaga tgaggtactg gcgaatgctg gagttgatgt
      601 ttttgaagag ttatttcaac ttatttttac taaactctat gatgaaatgg aaagtacacg
      661 caataaaagt cgttatttag agtttcgtaa ctatggtgat accgaaagtg aattaaaggc
      721 aaaaattcaa tctctatttg atcgtgcaaa aaataaatgg gaaggcgtat ttgctgaaag
      781 tgccaaaatc aacctttccc cttcgcattt ggcgatttgt gtatcttcat tggaagaagt
      841 aaagctgttt aattctaacc ttgatgtagt ggatgaagca tttgagtact taattaataa
      901 atccagtaaa ggagagaaag gacaatactt tacgcctcgt tacgtcattg atatgtgcgt
      961 aaaaatgctc gatccaaaac ctgaagaaac agtcattgat accgcagcag gaagctgtgg
     1021 ttttccagtt cattctattt ttcacgtttg ggaaaaacaa cttaaagcga gaggattaga
     1081 acgctcacac ttatttactg ctgaagaaaa attgcctgaa caaacagatt atgtgaaaga
     1141 aaaagtcttt gcgattgatt tcgatgaaaa ggcagtacgt gttgctcgta ccctaaactt
     1201 aatcgcagga gatgggcaaa ccaacgtact acatttaaat accttagatt atgaacgttg
     1261 ggaagatttt actaaggaag aagaatggaa tgatgtatat ggagaaggct ggaaaaaatt
     1321 aagaaaatta cgtaaaacta aaaatgaaaa tcgagatttc caatttgatg tgctaatggc
     1381 aaatccgcct tttgccggtg atattaaaga aactcgtata ttagctcgct atgaattagg
     1441 caaaaatagc aaaggaaaac agcaatccaa agtcggacga gatattttat ttattgaacg
     1501 caatttagat ttcttaaaag acggtggacg aatggcgatt gtgctaccac aagggcgttt
     1561 taacaattcc agcgataaat atattcgtga ttttattgca gagcgttgcc gcattttagc
     1621 tgttgtgggc ttacacggaa atgtgtttaa accacatacc ggcacaaaaa cttccgtact
     1681 atttgtgcaa aaatggcacg atgaactttg cccgaaagta gaggattacc cgattttctt
     1741 tgccacaatg cagaaatcca gcaaagataa ttcaggcgat aaaatctatg ttaaaaacgc
     1801 tgatggcaca ctcaaattag acaaccatca acacctaatt gtggagcacg atctatttaa
     1861 tcacgataat agtacccaag acggcattgc agaagccttt attgaatttg ctaaaaaaga
     1921 aaagctctct tttgtgggaa aggattaagc ccatttgacc ctgctaaata tcaggcattg
     1981 ttagatgggc tagaggtgag cgaggtttat ttgagtaagg ttcgttctgc aagttattcg
     2041 gaacgaatgg atagtacttt ctttttgaaa aaatacttaa accatccgag ttttaatttt
     2101 ccattatcgc tgaatgaggt ttctctgatt aaaagtggta caacaccaac agatcgagat
     2161 gataacttaa aagagggggt tgtcttatta aaaaccaatg atattaggaa taatctatta
     2221 aataagtact cttcggtaga ttattttatt agcgaagata tcaatgagaa aatgatttca
     2281 tcacaactga aagaagggga tgttttagta aatatcgttg gtgcaacttt ggaggttgtt
     2341 ggtcgggtag cttatgttag tagcactttt ccaaaagcaa atattacaca agcaatgt
//
LOCUS       DANLZC010000221         2370 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.223, whole genome shotgun sequence.
ACCESSION   DANLZC010000221 DANLZC010000000
VERSION     DANLZC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.223"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..516
                     /locus_tag="PXE44_002501"
     CDS             <1..516
                     /locus_tag="PXE44_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5589954.1"
                     /translation="KPSWFILAIIMTLRCTGIRRGQLVKLKIGDVNLSNRTIYISPEI
                     NKNHDYHIVPISDSLYLHIERLITELKIRKQTDNCQLFNLNLFSRCSRRRGQPMSQNQ
                     LTHIFRHISDLVGFTSSPHRFRHTVATQLMKKPENVYVVQKLLGHKDISVTLGYIEHD
                     VEMLRETVNLL"
     gene            697..1668
                     /locus_tag="PXE44_002502"
     CDS             697..1668
                     /locus_tag="PXE44_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISApl1 family transposase"
                     /protein_id="HDL5589955.1"
                     /translation="MSTSYRHLTINEREKIMILLAQGKKQAEIAKALGRSSSTISREL
                     KRHALESYSATNAQNSYLKHRQNSKAQRKLEQPEYFNLVQEKFLTENWSPEQISARLK
                     LEKSELSISYSTIYRGIYSGLFDIGERKASRKLRHKGKTRHTKNHHEKRGKIQISNHL
                     NDRPISAQNRSRFGHWKADTVLGKAGGACLLTLTERKSRFELVKKIPAKKAEAVQKAM
                     IELLDSHILRSITPDRGKEFAQHRLVTEALGVEFYFPEPHQPWTRGTNENTNGLLREY
                     FPKHQDINQWSEVDIQQVVNKLNLRPRKCLGWKTPYEVYFKKTLHLV"
     gene            complement(1941..>2370)
                     /locus_tag="PXE44_002503"
     CDS             complement(1941..>2370)
                     /locus_tag="PXE44_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5589956.1"
                     /translation="DIDMTSKIINIRAETNKNHEHHEIPISDKLYPYLKRLILESRAR
                     STSSDEQLFNINRFSLVTTSRNKKMTEGQLVHFFRHISEFIGFTVTSHRFRHTTATEV
                     LKQSGDIYAVKQLLGHKDLTVTLTYIHNDPEMLRKHVNSL"
BASE COUNT          798 a          429 c          445 g          698 t
ORIGIN      
        1 aaaccaagtt ggtttattct tgcgattatt atgacattga gatgtacagg cattcggcga
       61 ggacaattag tgaaactgaa aattggtgat gtaaatcttt ccaacaggac gatttatatt
      121 tcgccagaaa tcaataaaaa tcatgattac catatcgtac cgatttcaga ttcactttat
      181 cttcatatag aaaggcttat tacagagtta aaaatcagaa aacaaacaga taattgccaa
      241 ttgttcaatc tcaatttatt ttctcgatgc tctcgaagaa gaggacagcc aatgagccaa
      301 aatcagctta cgcatatatt tcggcatatt tctgacttgg tcggttttac ttcatcacca
      361 catagatttc gacatacggt tgcaactcaa ttaatgaaga agcccgaaaa tgtttatgtg
      421 gttcagaaac ttctagggca taaggatatc agcgtaacac taggttatat tgagcacgat
      481 gtggaaatgt tgagagagac agtgaattta ctctgataaa agaaaaatgg gagaatataa
      541 tttctcccac tttttttcta taaataaata atttctgttt tatcttctaa atgaactgat
      601 tttatacgct gaatttacaa tccaagtgca acaaaaaaag aagtactcat caactagaat
      661 ataattttgt ttccacacaa aaaccacttc caaatgatga gtacttccta ccgacatctt
      721 acaataaacg agcgagaaaa gataatgatt ttactcgcac agggcaaaaa acaagcagaa
      781 attgccaaag cactgggacg tagctccagc accatttctc gcgagctgaa acgacacgct
      841 ctagaaagct acagtgcaac gaacgcacaa aacagctatt tgaagcatcg tcaaaatagc
      901 aaagcacagc gcaaattaga gcagcctgaa tatttcaatt tggtgcaaga aaagtttctg
      961 acagaaaact ggtcgcccga acaaatcagc gcacgattaa aattggaaaa atctgaatta
     1021 tccattagtt attcaaccat ttatcgtggt atttattcag ggttgtttga tataggcgaa
     1081 cgcaaagcca gtcgcaaact gcgccacaaa ggcaaaacac ggcatacaaa aaatcatcat
     1141 gaaaaacgtg gcaaaattca gatatccaac catttgaacg accgtcccat ttcggcgcaa
     1201 aatcgcagtc gctttggaca ttggaaagcc gataccgtac tgggtaaagc gggtggagct
     1261 tgtttgctga cgctgacgga acgcaaaagt cgttttgagt tggtgaagaa aattcctgcc
     1321 aaaaaagccg aagcagtcca aaaagccatg attgaattgc tggattcaca tatattgcgg
     1381 tcaattacgc cagaccgtgg taaagaattt gcccaacatc gtttggtaac agaagcactg
     1441 ggtgtagaat tttacttccc cgagccgcat caaccgtgga cacggggaac gaatgaaaat
     1501 acaaatgggt tacttcgtga atactttccg aagcaccaag acatcaatca gtggagcgaa
     1561 gttgatattc aacaggtggt caataaactg aatttacgac cgcgtaaatg tttgggttgg
     1621 aaaacacctt atgaagttta cttcaaaaaa acgttgcact tggtttgaca attcaagaca
     1681 ataattttgt cacccttagg aaaattattc atggtcctaa gattcagtaa agattgtgga
     1741 ataaataagt gatcgtcatt tatcaatgtg agagcaaaga ctctggtttt agttctaaga
     1801 gctttatcaa aagaaaggtt taaataaatt gcacccgatg acaaagttat ctctggatta
     1861 attaaaaaat cggggctgac gtagactagc atacttacta atctacgtca gtcccttatt
     1921 ttatctatgt agcatcattt ctacaacgaa ttgacatgtt ttcttagcat ttcaggatca
     1981 ttgtgaatat atgttagagt tacagtgaga tctttatgtc caagtaattg cttcactgca
     2041 tagatatccc ctgattgctt taggacttct gttgcagttg tatgtcggaa tcgatgtgaa
     2101 gttactgtaa atcctataaa ttcagaaata tggcgaaaaa aatgaactag ttgtccttct
     2161 gtcattttct tatttcttga tgtagtaact aatgaaaagc gattaatgtt aaataactgc
     2221 tcatctgaac tagtgcttct agctcttgat tctaatatca atctttttag gtatggatat
     2281 aatttatctg aaattggaat ttcatggtgt tcatgattct tattggtttc agctcttata
     2341 tttataattt tgcttgtcat atcgatatca
//
LOCUS       DANLZC010000222         2349 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.224, whole genome shotgun sequence.
ACCESSION   DANLZC010000222 DANLZC010000000
VERSION     DANLZC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.224"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..739
                     /locus_tag="PXE44_002504"
     CDS             <1..739
                     /locus_tag="PXE44_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL5589957.1"
                     /translation="KTEEDKKTEEDKKTEEDKKTEEDKKTEEDKKTEEDKKTEEDKKA
                     EEAKKIAEAKGIGNKEYKDGLNELEEKNETGKNGEEIRTHGYLYNSHYSVVTAKMKQT
                     LQENGRQMEPTVEVKGLKTENLPTEGKATYKGEAFDSHGNNSNSVVGGELIYNVDFSS
                     RTGSGLVKNVQGGSIELAQGEIKNDSIIASAHQKYNDQAVGNGSYNIQFFGPNAEEIG
                     GKIELNGEGEGSMKQMLGIAGTREEQK"
     gene            788..2182
                     /locus_tag="PXE44_002505"
     CDS             788..2182
                     /locus_tag="PXE44_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5589958.1"
                     /translation="MYKKHFLFLLVSPLVMAETVEFQQDPKEFDGQRQAVKFEQEIAK
                     KDLKLTAYLEEDNSLKGIEQHINQAILRQDWAKLERLLGDYAKQEQFDPLLLDYALAA
                     LAYAKKDYRSAAFYYQQLLKKQPDLTYPRFDLALILKEDYRYREAEQEFKQLKTDDLR
                     NAVNSALAQIEEEESWKPDFYLQYTQTNNVNNASSSPTVNVNGRIFIKESDSLPQSAK
                     GVRYGVGLSKMRNIGGNHFVGVDLGYNGVYYWNNHDYSEQSVSLTPKYSYRTATYRIG
                     VSPFIEQNWLGSSRYNYQFGSSINAVKLFERGLISGSFSHLQKRYSEQHIADRYNGYR
                     NQLTLGTQWQAVKNWRFFANLNASREITREKAQSSNKWLATIGVIYQTPSWASSVSVG
                     YGKRYFADKHYLFGYKRQDKEYQANVAVWNRSWHWQGIVPKLNFRYQKIDSNIADFYS
                     RSNKEVFMTLEKLF"
BASE COUNT          823 a          385 c          527 g          614 t
ORIGIN      
        1 aaaaactgaa gaggataaaa aaactgaaga ggataaaaaa actgaagagg ataaaaaaac
       61 tgaagaggat aaaaaaactg aagaggataa aaaaactgaa gaggataaaa aaactgaaga
      121 ggataaaaag gctgaagaag caaaaaaaat tgcagaggca aaaggaattg gcaataaaga
      181 atataaagat ggattaaatg agttagaaga aaaaaatgaa acaggtaaaa acggagaaga
      241 gattcgaacc catggttatc tctataacag ccattattcc gttgtaactg ccaaaatgaa
      301 acaaacttta caggaaaatg gtcgtcaaat ggaacctacg gttgaagtga aagggcttaa
      361 aactgagaat ttaccgacag agggcaaagc aacatataaa ggagaagcct ttgattctca
      421 cggtaataac agcaattcag tagtaggtgg agaattaatc tataatgttg atttctctag
      481 tcgtaccgga tcaggtctag ttaaaaatgt acagggcggt tctatcgagt tagcacaagg
      541 cgaaataaaa aatgatagta ttatagcttc tgctcaccaa aagtataatg atcaggctgt
      601 tggaaatggt agttacaata tccaattctt tggtccaaat gcagaagaaa ttggagggaa
      661 aattgagctt aatggtgagg gcgaaggctc aatgaagcag atgttaggta ttgcaggaac
      721 ccgagaagaa caaaaataac atttaatacc caatgccgtt taatcttaaa cggcattctt
      781 ttttactatg tataaaaaac attttctatt tttacttgtt tcgcctttag taatggcgga
      841 aacggttgag tttcagcaag atcccaaaga gtttgatggg cagcgacaag cggttaaatt
      901 tgagcaagaa attgcaaaaa aggatttaaa attaaccgct tacttggagg aagataattc
      961 tcttaaaggt attgaacagc acattaacca agcgattctt agacaagatt gggcgaagct
     1021 ggaaaggctg cttggcgatt atgcaaagca agagcaattt gatccgctac ttttagacta
     1081 tgctttggcg gcattggctt atgctaaaaa agattatcgc tctgcggcgt tttattatca
     1141 gcagttgctg aaaaaacagc ctgatttaac ctaccctcgt tttgatttgg ctctgatttt
     1201 aaaagaggat tatcgctaca gggaagcaga gcaagaattt aagcaactca aaaccgatga
     1261 tttacgcaat gcggtgaaca gtgcgttggc tcaaatagag gaggaagaga gctggaagcc
     1321 ggatttttat ttgcaatata ctcaaaccaa caatgtgaat aatgcttctt cgtcgccaac
     1381 ggtaaatgta aacgggcgga tttttattaa agaatcagac agcttgccgc agtcggcaaa
     1441 aggcgtaaga tatggcgttg gcttgagtaa aatgcgaaat atcggcggta atcattttgt
     1501 gggagtcgat cttggctata acggcgttta ttactggaat aaccacgatt atagcgaaca
     1561 gtcggtctca ctaacaccaa aatatagcta cagaacggca acttatcgta tcggggtttc
     1621 gccctttatt gagcagaatt ggctgggtag ctcacggtat aactatcaat ttggtagcag
     1681 tatcaatgct gttaaacttt ttgaacgagg gctgatttcc ggctcattca gccatctgca
     1741 aaaacgttat agtgaacagc atattgccga ccgttacaat ggttatcgca accaactcac
     1801 gctaggtacg caatggcaag cggtcaaaaa ttggcgattt tttgcaaatc ttaacgctag
     1861 ccgtgaaatt acccgtgaga aagctcaatc ttccaataag tggctggcga ccattggcgt
     1921 aatttaccaa accccaagct gggcaagttc ggtaagtgtc ggatatggca agcgttattt
     1981 tgctgataaa cattatctgt ttggctataa acgccaagat aaggaatatc aagcgaatgt
     2041 ggcggtgtgg aatcgaagtt ggcactggca agggattgtg ccgaaattga atttccgtta
     2101 tcaaaaaatc gatagcaata ttgccgattt ctactctcgt agcaataaag aagtctttat
     2161 gaccttagag aaactatttt aaggctgtgt atgaatgtcg gggagaaaaa ttttctcccc
     2221 gatatttatg tgtagaagat cagacttgat ctgacaattc actctaaaaa gtgagagttt
     2281 cccgtttaga atatgagtgt caaaaatcaa tctaaacaaa aaggaaactc tcatgtttta
     2341 ttctaacaa
//
LOCUS       DANLZC010000223         2295 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.225, whole genome shotgun sequence.
ACCESSION   DANLZC010000223 DANLZC010000000
VERSION     DANLZC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.225"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            101..529
                     /locus_tag="PXE44_002506"
     CDS             101..529
                     /locus_tag="PXE44_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5589959.1"
                     /translation="MTHDWLYWALASALFAALTAIFAKVGLQGVDSDFATFIRTVVII
                     IALIAFLTYSGKWQPLDSLSSKNWLFLILSGLATGLSWSAYFKALQMGNASQVAPIDK
                     FSLVLVTLFAVIFLGERPSGQDWLGIGLVAGGVLLLAFKR"
     gene            535..1257
                     /locus_tag="PXE44_002507"
     CDS             535..1257
                     /locus_tag="PXE44_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDL5589960.1"
                     /translation="MQNFANIEIAFIHRKAPLPSDFSYPEMPGGLTEKQVQKWQSRRV
                     AFFLLTELFKKHHLDLDLLADMQRSPSGRTFVDSEQIDFNISHSGEWIAVIFSYSFAK
                     LAVGIDIEHPQKIRRYHDLICHYGNAEEQNVLLANHSPILSNLAERFYLSWCLREAIL
                     KSQGVGIVKLSEVKHLPLEKQIFSAHCPKGKLHFVSELPFYLSYFYQQPQNMLLSEPL
                     LYHWQNGQFQPIKCQSLVYDVN"
     gene            1267..1686
                     /gene="psiE"
                     /locus_tag="PXE44_002508"
     CDS             1267..1686
                     /gene="psiE"
                     /locus_tag="PXE44_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDL5589961.1"
                     /translation="MPKKSLEEHHSSFGKIASETFQWIMNIALLVTGALLSYSLFAEA
                     YSLYELSFSDSEKFQIVEKIVIFFLYFEFLALIVKYFKSNYHFPLRYFLYIGITAMVR
                     LIIVDHSNALHTLLFALSILVMIIALYIVHTDRLHKS"
     gene            1695..>2295
                     /locus_tag="PXE44_002509"
     CDS             1695..>2295
                     /locus_tag="PXE44_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL5589962.1"
                     /translation="MKLTQYQHNDLQILNRETVYKGHFELQKITFRHKLFSGEMSGEV
                     VRELLIKGEAAALIAYDPTLDKVVLIEQVRIGAYDAGQKKSPWLLELVAGMVDKGNEE
                     PAEVAMREAKEEAGLEVTTVQHALSVWDSPGGQLERLHLFLGLIDSTQVKSGEVHGLL
                     EEENEDILVHVVSREQAYQWVEDGTIDNVIAVIGLQWLQL"
BASE COUNT          661 a          460 c          483 g          691 t
ORIGIN      
        1 attttgcgaa gatcaaatag aaaatttgat aaactagaac taactcgcat tttattgctc
       61 catatgatta gaaattaacc taaaatatta aaattttgtt atgacacacg attggctcta
      121 ttgggcatta gcctctgcac tttttgcagc attaaccgcc atttttgcca aagttggctt
      181 acaaggcgta gattctgatt tcgccacttt tattcgcacg gtagttatta ttattgcact
      241 tattgccttt ctcacttaca gcggaaaatg gcagccgtta gatagcttga gcagcaaaaa
      301 ctggctgttt ttaatcttat ccggcttggc aacaggattg tcttggtcgg cgtatttcaa
      361 agcattgcaa atgggtaatg cttcccaagt cgctccaatc gataaattca gcttggtgtt
      421 agttaccctt tttgcagtaa ttttcttagg cgaacgccca agcggacaag attggcttgg
      481 cattggctta gttgctggtg gcgtactgtt acttgccttc aagcggtgat ttttatgcaa
      541 aattttgcaa atattgaaat tgcgtttatt catcggaaag cacctctgcc gagtgatttc
      601 tcctaccctg aaatgcccgg cggtttaact gaaaaacagg tgcaaaaatg gcaaagccgc
      661 cgagtcgcct tttttctgct gacggaattg tttaaaaaac accatctgga tttagattta
      721 ttagctgata tgcaacgctc cccaagcggt cgcacttttg tcgattctga gcaaattgac
      781 tttaacatca gccattcagg ggaatggata gcggtcattt tttcgtattc ttttgcaaaa
      841 ttagcggtcg gcattgatat tgaacaccca caaaaaatca gacgttacca cgatttaatc
      901 tgccattacg gaaacgctga agaacaaaat gttttattag ccaaccactc ccccattcta
      961 agcaacctcg ccgaacgctt ttatttgagc tggtgcttgc gagaggcgat attgaaatcg
     1021 caaggggtgg ggattgtgaa actgtccgaa gtaaaacatt taccgttaga aaaacagatc
     1081 ttttccgccc attgtccgaa aggcaagctg cattttgtga gcgaactgcc gttctattta
     1141 agctacttct atcaacaacc tcaaaatatg ctattatctg aacctttgct ataccattgg
     1201 caaaacgggc agtttcagcc tataaagtgc caatcacttg tttatgatgt taattaagga
     1261 taacgaatgc ctaaaaaatc tttagaagaa caccattcca gttttggcaa aattgcttcc
     1321 gaaacctttc agtggattat gaatattgcc ttgctagtaa caggagcatt gctttcttac
     1381 tcgctgtttg ctgaagccta ttcactctat gaactttcct ttagcgacag tgagaaattc
     1441 caaattgtag aaaaaatagt aattttcttt ctctattttg agtttcttgc gttgattgtg
     1501 aaatatttca aatcgaatta tcactttccg ttacgctatt tcctctatat cgggattact
     1561 gccatggtgc gtttgattat tgtcgatcat tctaatgcgt tgcatacact attattcgca
     1621 ctctctattt tggtgatgat tatagcatta tatattgtac acaccgatcg tttacataaa
     1681 agctaggaac taatatgaag ctaacccaat atcagcacaa tgatttacag attctaaacc
     1741 gagaaaccgt ctataaaggg cattttgagc tacaaaaaat cactttccgc cataagctgt
     1801 tttccggtga aatgagcggt gaagtggtgc gtgaactctt aattaaaggc gaagcggcgg
     1861 ctttaattgc ctatgatcca accttagata aggtagtatt aattgagcaa gtgcggattg
     1921 gagcttacga tgcaggacag aaaaaatccc cttggttgct ggaattggtt gccggtatgg
     1981 tagataaagg caacgaagag ccggcagaag tggcaatgcg tgaagccaaa gaagaagccg
     2041 gcttggaagt cacaacagtg caacacgcct taagcgtatg ggacagtccc ggtggacaac
     2101 tggaacgctt gcacctgttt ctcggcttga ttgacagcac ccaagtgaaa tccggcgagg
     2161 tacacggctt actggaagaa gaaaatgaag acattttggt tcacgttgta agccgtgaac
     2221 aggcatatca atgggtagaa gacggcacaa ttgacaatgt gattgcggta attgggctac
     2281 aatggttgca attaa
//
LOCUS       DANLZC010000224         2070 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.226, whole genome shotgun sequence.
ACCESSION   DANLZC010000224 DANLZC010000000
VERSION     DANLZC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.226"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(68..760)
                     /locus_tag="PXE44_002510"
     CDS             complement(68..760)
                     /locus_tag="PXE44_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5589963.1"
                     /translation="MILFAGDPHGSYEHLYPILQQQTEEVALVILGDLQLTTPDELDR
                     LSQYCDIWFIHGNHDSKTVAAFDAIWNSSWKERNIHGKVVDIQGIKIAGIGGVFRGHI
                     WMPPNRPMFFDPIHFCQYSAPEKIWRGGIPLRHRSSIFPSDFEALENEKADILITHEA
                     PKPHPQGFSVINQLARKLGASKIFHGHYHDNFNYGNLNVSRECAIYNIGFRSLADING
                     NYLILGVDDREK"
     gene            complement(778..2052)
                     /gene="nadR"
                     /locus_tag="PXE44_002511"
     CDS             complement(778..2052)
                     /gene="nadR"
                     /locus_tag="PXE44_002511"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDL5589964.1"
                     /translation="MTDFAYLQQKRKQLKLKVNDICEQAGVTRAYFNQLVSGKIKNPS
                     ANKLKALHSVLDIVEDSNQRSGVIFGKFYPIHTGHINMIYEAFSKVDVLHVIVCTDSE
                     RDLRLFQESKMKRMPTNEDRLRWVQQIFKYQQKQIFIHHLVEDGIPSYPNGWEDWSGR
                     VKALFEEKSINPTIVFSSEIQDKESYEKYLNLEVQLVDPTRESFNISATQIRNNPFQY
                     WRFIPKEVRPFFVKTIAILGGESSGKSVLVSKLANVFNTTSAWEYGREFVFEQLGGNE
                     QAMQYSDYPQMVLGHQRYVDYAMKHAHKVAIIDTDYITTQAFCIQYEGKAHPFLDSMI
                     REYPFDVTILLSNNTKWVADGLRSLGSKKQRQQFQHLLKKLLEKHHIPYIKIESPSYL
                     DRYNQAKEVVEAILNEEPIPLQFKQYNIHEFE"
BASE COUNT          628 a          397 c          375 g          670 t
ORIGIN      
        1 ttttacttat aaatttatga atgggaaggg taaaatgaca atttgtttaa ttcagtcaaa
       61 tttttggcta tttctctcga tcatcaacac ctaaaattag ataatttcca ttaatatccg
      121 ctaggctacg aaagccgata ttataaatag cacattctct gctgacattt agattgccat
      181 aattaaaatt atcatggtag tggccatgga aaattttgct cgcccctaat tttcttgcta
      241 gctggttgat tacagagaag ccttgagggt gtggctttgg tgcttcgtgt gtaattaaaa
      301 tatcggcttt ttcattttcc aatgcttcaa aatcagaagg gaagatcgaa gagcggtgcc
      361 ttaatggaat accaccacgc caaatttttt ccggagcaga gtattggcaa aaatggatgg
      421 gatcaaaaaa cattggacga ttgggcggca tccaaatatg acctcggaag acccctccaa
      481 tacctgcaat ttttattcct tgaatgtcaa ctactttacc gtgaatattg cgttctttcc
      541 agctactatt ccaaatagca tcaaaggcag caacggtttt actgtcatgg ttaccatgaa
      601 taaaccaaat atcacaatac tgcgaaagcc gatctaattc atcaggagta gtcaattgaa
      661 gatcgcctag aataactaaa gctacctcct ccgtctgctg ttgtagaatc ggataaagat
      721 gctcgtagct accgtgtgga tcgcctgcaa aaagtatcat gttatttcct gtggcagtta
      781 ttcaaattcg tgaatattat attgcttaaa ctgtaatggg attggttctt catttaaaat
      841 agcttctacc acctctttgg cttgattgta gcgatccaaa taacttggag attcaatttt
      901 aatataagga atatggtgtt tttctagtag ttttttcagt aggtgttgaa actgttgacg
      961 ctgttttttc gatcctaaac tgcgtaaacc atcggctacc cattttgtat tgttagaaag
     1021 taaaatcgtc acatcaaaag gatattcgcg gatcattgaa tccagaaacg ggtgagcctt
     1081 cccttcatac tgaatacaga acgcttgagt tgtgatgtaa tcggtatcaa taatagccac
     1141 tttatgagca tgtttcatgg cataatcaac gtagcgttga tgtccaagca ccatttgcgg
     1201 atagtcggaa tattgcattg cttgttcatt gccaccaagt tgctcaaaca caaactcacg
     1261 cccatattcc caagcagagg tagtgttaaa tacattcgct aatttgctca ccaacactga
     1321 tttaccactg ctttcaccac ctaaaatcgc aatagtttta acaaagaaag gacgaacttc
     1381 tttcggaata aagcgccagt attgaaaagg attattacga atttgtgttg ctgaaatatt
     1441 aaaggattca cgagtaggat cgacaagttg aacttctaga ttcaaatatt tttcataaga
     1501 ttctttatct tgaatttcac tactaaaaac aatagtagga ttaatgcttt tttcttcaaa
     1561 tagcgcttta acacgaccag accaatcttc ccagccattt ggatagctcg gaataccgtc
     1621 ttcaactaaa tggtgaataa agatctgttt ttgttggtat ttaaaaattt gctgtaccca
     1681 gcgtagccga tcttcatttg tcggcattcg tttcatttta ctttcctgga aaagacgtaa
     1741 atcccgttca gaatcggtac aaacaataac atgtaaaaca tctactttac taaatgcttc
     1801 atagatcata ttaatatgac ctgtgtgaat aggatagaat ttgccaaaaa taaccccgga
     1861 acgctggttg ctatcttcta cgatatctaa aacggaatgc aaggctttaa gcttattggc
     1921 gcttggattt ttgatttttc cgcttactaa ctgattaaaa taagctcggg ttaccccagc
     1981 ttgttcacaa atatcattca ctttgagttt aagctgtttg cgcttttgtt gaagataagc
     2041 aaaatctgtc ataatgattc cctcgatttt
//
LOCUS       DANLZC010000225         2044 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.227, whole genome shotgun sequence.
ACCESSION   DANLZC010000225 DANLZC010000000
VERSION     DANLZC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.227"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(35..325)
                     /locus_tag="PXE44_002512"
     CDS             complement(35..325)
                     /locus_tag="PXE44_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589965.1"
                     /translation="MNNLSEFKLRYINNPYSNKDTFFIFDELSILLKSLTSDELKKIG
                     TAKNLADRQLNKQRKRCKRYVSEAYRLVEDNKEPYSKFFNHDGITAYNIKAF"
     gene            complement(375..1139)
                     /locus_tag="PXE44_002513"
     CDS             complement(375..1139)
                     /locus_tag="PXE44_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5589966.1"
                     /translation="MPNITPKLVINALEPAKEKGIGDTLKRAIRLLNEVMNFAVNADV
                     IEFNKCQNVGSNFSVAKSEHNPTIHPKELPQFLAALRDYNLSFQTKMLIKWQLLTMTR
                     SREASETLWSEIDLEAKTWTISAERMKMKRPHVIPLSRQAVEILERMRKITGNSPFVF
                     QSEMKPRQPMNSQTANRAIGLLGYAGTLTAHGMRSIASTYLNEQLVNYDVVEACLAHV
                     IKDQTRKAYNRSDYLDQRVDVMQQWADYVESCSTGI"
     gene            complement(1108..1587)
                     /locus_tag="PXE44_002514"
     CDS             complement(1108..1587)
                     /locus_tag="PXE44_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDL5589967.1"
                     /translation="MARVTTELNNTQIKNAKAQTKDYKLMDGKGLFLLVKKNGSKLWR
                     FRYKKPITGKESDLGIGSYPETTLAQARTIREEYRALLAENIDPLEHRQRLEAEALAE
                     IENTFYKVCLKWRDKCESRVKNGELQALTLKKNWRILEKYLLPCRLLYSKHHAKTSY"
BASE COUNT          562 a          394 c          377 g          711 t
ORIGIN      
        1 ttcaggacga tataaaaaag gctctctaaa tagattagaa agccttgata ttgtatgcgg
       61 ttatcccatc gtggttgaaa aacttagagt aaggttcttt attatcttca acaagacgat
      121 aagcctctga tacatacctt ttacatcttt ttctttgctt attaagttgt ctatctgcca
      181 gatttttagc tgttcctatt ttctttaatt catcggaagt cagtgatttt aatagtatgc
      241 ttaattcatc aaatataaaa aatgtatctt tattagaata agggttattg atatatcgta
      301 atttaaactc agaaaggtta ttcatatttg tacacctcta attaaacaag cggtcaaatt
      361 ccgccaaaaa tttactaaat ccccgttgag cagctttcta cataatccgc ccattgctgc
      421 attacatcta cacgctgatc taaataatca gaacggttat aagcctttcg ggtttggtct
      481 ttgataacgt gagctaaaca agcctctact acatcataat tgactaactg ctcattcaaa
      541 taggttgagg caatagaccg cataccgtga gccgttagag tgccggcata gcctaataag
      601 cctatggctc tgttggcggt ttggctgttc atcggttggc gtggtttcat ttcgctttga
      661 aaaacaaatg ggctattccc tgtgattttt cgcattcttt ctaaaatttc gaccgcttgc
      721 cgtgaaagag ggataacgtg agggcgtttc atcttcattc tttcggctga aatggtccac
      781 gttttcgcct ctaaatcaat ttcactccac aacgtttcgc ttgcctctcg tgagcgtgtc
      841 attgttaata gttgccattt aatcaacatc ttagtttgaa atgataggtt gtaatcacgc
      901 aatgcagcta aaaattgagg tagctctttt gggtgtatcg ttgggttatg ttcagatttc
      961 gctacgctaa aatttgagcc tacattttgg catttgttaa actcgataac gtccgcattt
     1021 accgcaaaat tcatcacttc attaagtagc ctaattgctc gtttaagggt gtcgcctatg
     1081 cctttttctt tagcaggctc taaagcatta ataactagtt ttggcgtgat gtttggaata
     1141 gagtagtcgg caaggcaaaa gatacttttc aaggattcgc caattcttct tcagcgttaa
     1201 ggcttgaagt tcgccatttt ttacacggct ttcgcattta tcacgccatt tcaagcagac
     1261 tttataaaag gtgttttcta tttcggctaa cgcttcagcc tctaatcttt ggcggtgttc
     1321 gagtgggtcg atattttccg ctagtaatgc cctatattct tctctaatcg ttcttgcttg
     1381 ggctagggta gtttcggggt aactgcctat gcctaaatcg ctctctttgc cggtaatcgg
     1441 ctttttatac ctaaaccgcc ataacttcga gccgtttttc ttaaccagca aaaacagccc
     1501 cttgccgtcc attagcttgt agtctttggt ttgagccttt gcgtttttga tttgagtgtt
     1561 atttaattct gtggtaactc tagccataaa aatttaccta aaatcgcatt gtgctttatc
     1621 agaatcataa gggatttaag ggtatgggtt tcataccctt ttacgttata ccctcaaata
     1681 tacccttaaa ataaggggat gtaaacgcaa gaacacgaaa gaatacgaat gaagaaataa
     1741 tttaaaattg ctctaaagcc agtatcatca atggttttgc gtaagaatgc gaatgaagaa
     1801 ataagagtgg aaaatttaag ttggtcccag cctcctctga actcgcttat aacttattga
     1861 tttataaaga gataaaataa actctacaaa aatagggatc attttaggga tcatttacat
     1921 aaaagctatt tattttcgtt tataaaatgt tcatttgtgc ggctaggttg gtttgtcttt
     1981 cctagttgca tttctattct attatcagca actattcagc ccttcagcaa gatttcattt
     2041 ttca
//
LOCUS       DANLZC010000226         2003 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.228, whole genome shotgun sequence.
ACCESSION   DANLZC010000226 DANLZC010000000
VERSION     DANLZC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.228"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            19..1671
                     /locus_tag="PXE44_002515"
     CDS             19..1671
                     /locus_tag="PXE44_002515"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDL5589968.1"
                     /translation="MNGARFITESLKAHGVTTLFGYPGGAIMPTYDAIYDSGLDHLLC
                     RNEQGAAMAAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASH
                     LIGTDAFQEADVLGLSLACTKHSFIVQNIEELPEILALAFQIAQSGRPGPVLVDIPRD
                     VQLAQTSALPIVYPKEPADAQDPNKLVVAKQLLATAKRPVLYVGGGVSMADGVQAVRN
                     FVKIANIPSVSTLKGLGTIDPTDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDR
                     VTGKLDTFAPHAKVIHIDIDNAEINKLRQAQVALKGNLIEAVNYLAQPLDIEPWREDV
                     RNLKQQFDFQYQENSGDGNIDAVALLHTLSQRKAKNSIVTTDVGQHQMWSAQHLTYHD
                     PKNYITSAGFGSMGFGLPAAIGAIKARAEDQVILITGDGSIMMNIQELGTLKRADLAV
                     KILLLDNQRLGMVRQWQSLFFHGRHSQTILDDNPDFVLLAKAFDIDGERIEKASEVSD
                     AIDRMFNAKGAYLLHVCIPQEDNVWPLVPPGACNADMLDDEV"
     gene            1681..1899
                     /gene="ilvM"
                     /locus_tag="PXE44_002516"
     CDS             1681..1899
                     /gene="ilvM"
                     /locus_tag="PXE44_002516"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDL5589969.1"
                     /translation="MEEYQLTLKANKRPETLERILRVIRHRGFEVKCLNVESTDSAFN
                     LNFTLASKRPLSLLSHQLEKLFDIIEIN"
BASE COUNT          571 a          424 c          443 g          565 t
ORIGIN      
        1 attaaggaca caacatccat gaatggagca agatttatca ccgagagttt aaaagctcac
       61 ggggtaacga cactttttgg ctatccgggc ggggctatta tgcctaccta tgatgcaatt
      121 tatgattcag gcttagatca tttactttgc cgcaatgaac aaggtgctgc aatggcagct
      181 atcggttatg cccgtgcaac aggtaaagta ggggtttgta ttgcgacctc cggccctggg
      241 gcaactaatt taatcaccgg tttaggcgat gccgcacttg attctattcc tgtggtagca
      301 attaccggac aagttgccag ccatttaatc ggcactgatg ccttccaaga agctgatgta
      361 ctaggtttgt cactcgcttg cactaaacac agttttattg ttcagaatat tgaggagctc
      421 cctgaaattt tagccctcgc attccaaatt gcacaaagtg gcagacctgg tccggtgctg
      481 gtcgatattc cacgtgatgt gcaactagct caaacttcgg ctctgcctat cgtttatcct
      541 aaagaacctg ccgatgcaca agatcccaac aaattagtgg ttgccaaaca attattagcc
      601 accgccaaac gcccggtact ttatgtcggc ggtggcgtta gtatggctga tggtgtacaa
      661 gcggtcagaa attttgtaaa aattgcaaat attccgtctg tttctacctt aaaagggtta
      721 ggcactatcg atccaaccga tccgctctat atgggaatga tcggtatgca cggcacaaaa
      781 gcagcaaatt acgcggtaca agagtgtgat ttactcattg cctgtggtgc ccgctttgat
      841 gaccgtgtta ccggcaaatt agacactttt gcccctcacg ctaaagtgat tcacattgat
      901 atcgacaatg cggaaatcaa caagctccgt caagcacaag ttgccttaaa agggaatttg
      961 attgaagccg tgaattactt ggctcaaccg ttagatattg aaccatggcg tgaagatgtg
     1021 cgtaatttaa agcagcaatt tgactttcaa tatcaagaaa atagtggtga tggcaatatt
     1081 gatgccgtgg cgttattaca cacgctttca cagcggaaag ccaaaaacag catcgtcact
     1141 accgatgtag ggcagcatca aatgtggtcg gcacaacatt taacttatca cgatccgaaa
     1201 aactatatca cctctgccgg ttttggctct atgggttttg gcttgcctgc ggcgattggg
     1261 gcaatcaaag ctcgagcgga agatcaggtt attttaatta ccggtgatgg ctcgattatg
     1321 atgaatattc aagaactcgg cacgcttaaa agagctgatt tagcggtcaa aatcctatta
     1381 ctggataacc aacgtttagg tatggttcgc caatggcaat cgctgttctt ccacgggcga
     1441 cacagccaaa ccattttaga tgataaccct gattttgtgt tgttggcgaa agcctttgat
     1501 attgacggtg agcgaattga gaaagcgagc gaagtgtcag atgcaataga cagaatgttc
     1561 aatgccaaag gggcttactt acttcacgtt tgtattccac aggaagataa cgtttggcca
     1621 ctggtaccgc cgggggcgtg taatgccgat atgttagacg atgaggtata ggagaataag
     1681 atggaagaat atcaattaac gcttaaagct aacaaacgcc ctgaaacctt agagcgtatt
     1741 ttacgggtta tacgtcaccg tggttttgag gtgaaatgct taaatgtaga atcaacagac
     1801 agcgccttta acctaaattt tacattagca agtaaacgtc cactctctct tttaagccat
     1861 caattagaaa aactgtttga tatcattgaa atcaattaat cagacttttc attcgccgtg
     1921 taaaaaaata cacggctttt ttatgcttaa aatttatcat tcgctatcct tttgatttta
     1981 ttcatttctt caaatttcaa ctc
//
LOCUS       DANLZC010000227         1994 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.229, whole genome shotgun sequence.
ACCESSION   DANLZC010000227 DANLZC010000000
VERSION     DANLZC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.229"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            347..1879
                     /locus_tag="PXE44_002517"
     CDS             347..1879
                     /locus_tag="PXE44_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDL5589970.1"
                     /translation="MNLVDYSTSLWSVLPALIALILAIVTRKVIISLSIGILFGAFML
                     TNSNFLDALTYIKNSVVSLVYKGAEEGLNSNNVNIILFLLLLGVLTSLLSISGSNQAF
                     ANWAQKKVKGKRGAKLMAAGLVFFTFIDDYFHSLAVGAIARPVTDKFRVSRAKLAYIL
                     DSTAAPMCVLMPISSWGAYIITLIAGLLATHSITGYSPLGAFMTMSAMNFYAIFAMIM
                     VFFVAYFSFDIGSMARFERQAAHLAELDDEQIETKGHVRDLILPIIALIVATVSMMMK
                     TGADALAESGASFSILGAFENTTVGISLVVGGVAGLIVATICIITSGNISFKNYVRAY
                     GLGVKMMSGAITILFFAWTINGVVSDMQTGKYLSGLVAESIGAEFLPAILFVLAAAMA
                     FSTGTSWGTFGIMLPIGASMAVHSDPALIIPCLSAVMAGAVCGDHCSPVSDTTILSST
                     GAQCNHMDHVISQLPYALLVATASIIGYLVVGFTQSAVLGFIATAVVMAILILLFKEK
                     KH"
BASE COUNT          548 a          323 c          425 g          698 t
ORIGIN      
        1 aaaaaaatag atactttata tctaaagata ttcgtgctat cttaaacact gtcctacaaa
       61 ttgtagaggt gcgaattcaa taagtatttc ttctgagtgg aaaacgatga aggggaagga
      121 aaggtgaatt tgccgaaatc aattaagcgt catcttaatt ggttggggtc gttgtcgaaa
      181 gaaacgacac tgtcgtagta aatgctacgg agtgctactg ttagggtggg tttcagatat
      241 ttcacatttg ccttcctttc atatttattt ttataaatat cccagtagat ctcttgtttg
      301 gctattctta tttatacaaa ttatacacag tcattttaga ggttttatga atctagttga
      361 ttattctacg tcactttggt cggtattgcc ggcactgatt gcgctaatat tggcaattgt
      421 gacacgcaag gtgattattt cacttagcat cggtattctc tttggtgcat ttatgcttac
      481 taatagcaat tttcttgatg cacttactta tattaaaaat agcgttgttt ctttagttta
      541 taaaggggct gaagaaggat taaattcaaa taatgttaac attattttat ttttgctttt
      601 attaggggta ttaacgtcat tattaagtat ttcaggtagt aatcaggcgt ttgcgaattg
      661 ggcgcagaaa aaggtaaaag gcaaacgagg cgcaaaatta atggcggcag gtttggtttt
      721 ctttaccttt attgatgact atttccacag tttagcagta ggtgcgattg cccgccctgt
      781 tacggataaa ttccgtgttt ctcgtgctaa attagcttat attttagatt ctaccgccgc
      841 tccaatgtgc gtgttaatgc caatttcaag ctggggggct tatattatta ccttaatagc
      901 tggcttattg gctacgcatt ccatcacagg ctattcgcca ctaggcgcat ttatgacaat
      961 gagcgcaatg aacttttacg ccatttttgc gatgattatg gtgttctttg tggcatattt
     1021 ctcatttgat attggttcga tggcgcgttt tgaacgtcaa gcagcgcatt tagcagaatt
     1081 agatgatgag caaattgaaa cgaaaggaca cgtgcgcgat ttgattttgc caattattgc
     1141 acttattgtt gctacagtct caatgatgat gaaaacaggt gcagatgcac tggccgaatc
     1201 aggtgcttca ttttctatac taggtgcttt tgaaaatact acggtaggca tttcattagt
     1261 tgttggtggt gtggcagggc ttatagttgc aactatttgt atcattacaa gcggtaatat
     1321 ttcttttaaa aattacgtcc gtgcttatgg cttaggtgtg aagatgatgt ctggtgcaat
     1381 cactattctc ttctttgcgt ggacaataaa cggcgtagta agtgatatgc aaacagggaa
     1441 atatctatca ggattggttg cagaaagcat tggagcagag tttttacctg caattctatt
     1501 tgtgttagcc gcagcaatgg cgttttcaac aggaactagc tggggaacat ttggtattat
     1561 gttgccaatt ggagcctcaa tggcagtgca ttcagatcca gcattaatta ttccttgttt
     1621 atctgcagta atggctggtg cggtttgtgg tgatcactgt tctccagtat ctgataccac
     1681 tattctttct tcaacaggcg cacaatgtaa tcatatggat catgtcattt ctcaattacc
     1741 ttatgcttta ttagttgcaa cagcttctat tattggttac ttagtggttg gctttaccca
     1801 atcagcggta ttgggattta ttgctacagc tgttgttatg gcaatactca ttttgctttt
     1861 taaagaaaaa aagcattaat ttccgaaaat gtgaagggaa tcacagaatg agatttattg
     1921 atgagataaa atagcactca agatatggat atcgaatgtt gtgttatata aatcttaaaa
     1981 taagatcctc ctag
//
LOCUS       DANLZC010000228         1917 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.230, whole genome shotgun sequence.
ACCESSION   DANLZC010000228 DANLZC010000000
VERSION     DANLZC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.230"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            246..1196
                     /gene="coaA"
                     /locus_tag="PXE44_002518"
     CDS             246..1196
                     /gene="coaA"
                     /locus_tag="PXE44_002518"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL5589971.1"
                     /translation="MSLEKTSKITPFLTFDRQQWADLRKSVPLKLTEQDLKPLLGFNE
                     DLSLEEVRTIYLPLARLIQYYIEENLKRQTVLHRFLGIEPPKVPYIISIAGSVSVGKS
                     TSARILQALLSHYPKERKVDLITTDGFLYPLATLTEKNLLKKKGFPESYDIHSLIRFV
                     SDIKSGKRNVKAPIYSHLTYDIIPNQFNTVDQPDIVILEGLNVLQSGMNYPSSPHNVF
                     VSDFVDFSIYVDAEESLLKKWYINRFLKFRRSAFADPNSYFHHYASLSEQEAIETAST
                     IWEEINGLNLRQNILPSRERANLILIKGQDHAIETVKLRK"
     gene            1349..1888
                     /locus_tag="PXE44_002519"
     CDS             1349..1888
                     /locus_tag="PXE44_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HDL5589972.1"
                     /translation="MNKKFLLPLILFLALSVAFLVQLARNSQGDDPKKLESALIGKTV
                     PSFNLESVLDEKQTIDQNIVKTGKPRLLNVWATWCPTCYAEHEYLTILAKQGVEIVGI
                     DYKDERPKAIKFLANYGNPYKAIIFDPKGSLGLDLGVYGAPETFIIDGNGVIQYRHAG
                     DVNDQVWNQVLKPIYDGLK"
BASE COUNT          602 a          390 c          348 g          577 t
ORIGIN      
        1 ttgctgtgtc gtaagcaagt gatgtgcatt atagggaaat tttaacagcg tgcaagtgtt
       61 ttttaaaaaa atattaaaaa aagcgttctt tcgattttta aatatacaaa atgactattt
      121 tttgtaaaat acaacggatt ttctctgcat aactacggca aaataagtgc atatattgta
      181 tagttacgcc cttaatttgg tatttaatta ccttaatatt ccagcaaaaa attgagattt
      241 tggctatgag ccttgaaaaa accagcaaaa ttactccttt tctcactttt gatcgccaac
      301 aatgggctga tttacgcaaa tccgttccat tgaagctgac cgagcaagac ctaaaaccgc
      361 ttttaggctt taatgaagat ctttcattag aagaagtacg cactatttat ttaccgcttg
      421 cccgtttaat tcaatactat atagaagaaa acttaaaacg ccagacagtc ttacatcgct
      481 ttctcggcat tgaaccgcca aaagtccctt acattattag tattgccggc agcgtatcgg
      541 ttgggaaaag tacctctgcc cgtattctgc aagctctgct ctcccattac cctaaagagc
      601 gaaaagtgga tttaattacc accgacggtt ttttatatcc acttgcaacg ctaaccgaaa
      661 agaacttact caagaaaaaa ggctttccgg aatcttatga tattcacagc ctgattcgct
      721 ttgtatctga tattaaatcc ggcaaacgca atgtgaaagc tccgatttat tcacatttga
      781 cctacgatat tatcccaaat caatttaata cggtggatca acccgatatt gtgattttag
      841 aagggctaaa cgtgttacaa agtggaatga actacccgtc cagcccacat aatgtatttg
      901 tatcggactt tgtggatttt tctatttatg tggatgccga agaaagtttg ctgaaaaaat
      961 ggtatatcaa ccgtttcttg aaattccgcc gcagtgcctt tgccgaccca aactcctatt
     1021 tccaccatta tgcgagtttg tccgagcaag aagcgattga aaccgcctca acgatttggg
     1081 aagagatcaa cggcttaaat ttacgccaaa atattctccc aagccgagag cgtgccaacc
     1141 tcattttaat caaagggcaa gaccacgcca ttgaaacggt aaaattacgc aaataatatt
     1201 ttgcaaataa ttgggctttt tcgaccgctt gtaatacctt aaaatccgtt gaataccact
     1261 caacggattt tttatttaaa tcaatgctta ttcaactatt ttttggtaag ctaacgactt
     1321 tgagagtgat tgatattagg catatacaat gaacaaaaaa tttctacttc ctttaattct
     1381 ctttttggcg ttatctgtgg catttttggt tcaactcgct cgcaactcgc aaggcgatga
     1441 tccgaaaaaa ttagaatcag ccttaattgg caaaacagtt ccgagcttta atttagaatc
     1501 ggtattagat gaaaaacaga caatcgacca aaatatcgtc aaaaccggca aacctcgcct
     1561 gcttaacgtg tgggcaactt ggtgtccgac ttgttatgcc gaacacgaat acttaaccat
     1621 tttggcaaaa caaggggtgg aaattgtcgg tattgattat aaagacgaac gcccaaaagc
     1681 gattaaattc ttagccaatt acggcaaccc ttataaagcg attatttttg acccgaaagg
     1741 ctcactcggt ttagatttag gtgtttatgg tgcacccgaa acctttatta ttgacggcaa
     1801 cggcgtgatt caataccgcc acgccggcga tgtaaacgac caagtgtgga accaagtgct
     1861 taaaccgatt tatgatggat taaagtaaat gtttgtattt cctgctgaca ttaagaa
//
LOCUS       DANLZC010000229         1894 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.231, whole genome shotgun sequence.
ACCESSION   DANLZC010000229 DANLZC010000000
VERSION     DANLZC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.231"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(344..1585)
                     /locus_tag="PXE44_002520"
     CDS             complement(344..1585)
                     /locus_tag="PXE44_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDL5589973.1"
                     /translation="MMNKRIYVFADWETFSQPELVGVLSSDVVRGKENFRFSYDNEWL
                     SSPYVQQIDPNLYLYPGEQYSTDTQNFHIFLDSCPDRWGRLLMKRREAALASQENRKP
                     KLLIESDYLLGVHDMNRMGALRFKTELNGPFLDNNTRLAAPPLSSLRELEFAAIQLES
                     SDNVDDPDYLKWLHMLISPGSSLGGARPKASVIDELNQLWIAKFPSRYDDYDIALWEY
                     LAYRLAIQAKIEMATCRIERFNQEHHTFLTQRFDRHCGKRRHFTSALTQLGYYDGDYE
                     ASYLEIAQFLTESGSNTRIDLAQLWRRIVFNIAISNTDDHLRNHGFIYAQEGWILSPA
                     YDLNPVPLSQGLHLNITDSDNRLDYQLAFDVADFFQLSKQQAVQIYDEVLKEINNWQN
                     VAKEIGIPRQEQEQMKFAFNV"
     gene            complement(1585..1788)
                     /locus_tag="PXE44_002521"
     CDS             complement(1585..1788)
                     /locus_tag="PXE44_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5589974.1"
                     /translation="MFTQTLLAQRTGLSRVTVRKIMNGDPTVAIGYYLMVLGVLGLEQ
                     DFTDVGKDDELGRKLQDIELLRK"
BASE COUNT          551 a          381 c          331 g          631 t
ORIGIN      
        1 aaatgttagt ttttctgatg tctgccatct gctaggcatt atatatgcct agattagttt
       61 cctacatttg cgtttttaag gctacgcttc cttgcggata tggttgagaa aaaagtcaag
      121 tgttactaat ggttttataa gatgttggtt tgttctggtg tctgccaccg ctaggtcttt
      181 ataccctaga ttagtatcag gtctttctgg tgcctgccac cgctaggcct ttacatccta
      241 gattaatatc agattgacaa tgagccaaca actcattgcg ttattaaggt aacgctactt
      301 ttttagaaca tagactacga tgattggttc cacaagtcaa ttactataca ttaaatgcaa
      361 atttcatctg ctcttgctct tggcgtggaa taccaatttc ttttgcgaca ttttgccaat
      421 tattaatttc tttcagtact tcatcataaa tctgtacggc ttgttgctta gataattgaa
      481 aaaagtcagc aacatcaaag gcgagctgat aatccaatcg gttatcgctg tctgttatgt
      541 ttaaatgtaa tccttgagat agtggtactg ggtttaaatc gtatgcggga gataatatcc
      601 acccctcttg agcgtagata aatccgtggt tacgcaaatg atcatcagta ttcgaaatag
      661 caatattgaa tacaattctt ctccatagtt gagccaagtc aattcgagta tttgaaccac
      721 tttctgttaa aaattgagca atctcaaggt agcttgcctc atagtctccg tcataataac
      781 ccagctgggt taatgctgac gtaaaatggc gacgttttcc acaatgtcga tcaaatcttt
      841 gtgtcaaaaa agtatggtgt tcttggttaa agcgttcaat tctacaagta gccatttcaa
      901 ttttggcttg aatagccagt cgataagcta aatattccca aagagcaata tcataatcat
      961 cgtaccgact tggaaatttg gcaatccata gttgatttag ttcatctatt accgaagctt
     1021 ttggtctagc tccgcctagt gacgaccctg gtgatatcaa catatgaagc cattttaaat
     1081 aatccggatc gtccacatta tcactgcttt caagttgtat tgctgcaaat tctaattctc
     1141 ttaaggaaga cagtggtggt gctgcaagac gagtattatt gtccaaaaat ggaccattca
     1201 attcagtttt aaaacgtaaa gcccccattc tattcatatc gtgtacgcct aaaaggtaat
     1261 cactttctat tagtaactta ggtttacgat tctcttgact agctagagct gcctcccgac
     1321 gtttcattaa caatctaccc catctatcag ggcaagaatc taaaaagata tgaaaatttt
     1381 gtgtatctgt actgtattgc tctccagggt aaaggtataa gttaggatca atctgctgta
     1441 catagggtga acttaaccac tcgttatcgt agctaaaacg gaaattttct ttgcctctca
     1501 ctacatcaga agaaagcacc ccaactaatt caggctgtga aaaagtttcc caatctgcaa
     1561 acacataaat tcgtttattc atcattattt ccttaataat tcaatatctt gcaatttacg
     1621 tcctaactca tcatcctttc caacatcagt gaaatcttgt tctagaccga gaacacctaa
     1681 aaccattagg tagtatccaa tcgcaacagt tggatcacca ttcataattt ttctcactgt
     1741 tacacggcta agcccagttc tttgagccaa taatgtttga gtaaacattc tcctttttga
     1801 ggctagccta atattttctc caagttgaag caaggcattt tggtacttag gaaacagagt
     1861 tggtgatttt gttttactga gcattattat tacc
//
LOCUS       DANLZC010000230         1842 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.232, whole genome shotgun sequence.
ACCESSION   DANLZC010000230 DANLZC010000000
VERSION     DANLZC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.232"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            38..478
                     /gene="nudB"
                     /locus_tag="PXE44_002522"
     CDS             38..478
                     /gene="nudB"
                     /locus_tag="PXE44_002522"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDL5589975.1"
                     /translation="MNYKNPHSILVVIYAKNTQRVLMLQRKDDPDFWQSVTGSINIGE
                     TPLEAAEREVSEETGLQTFEQKQPLVDCQKQIEFEIFPHFRYKYAPNITHCVEHWFLL
                     PLDSEQEPILTEHLAYRWVSVEDAVRLTKSPNNAQAIQEYLTSK"
     gene            491..1231
                     /locus_tag="PXE44_002523"
     CDS             491..1231
                     /locus_tag="PXE44_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5589976.1"
                     /translation="MAGHSKWANIKHRKAAQDAQRGKIFTKLIRELVTAAKLGGGDTS
                     SNPRLRAAVDKALSSNMTRDTINRAIERGVGGGDDTNMETKIYEGYGPGGTAVMVECL
                     SDNANRTISQVRPSFTKCGGNLGTEGSVGYLFSKKGLILVANADEDALTEAAIEAGAD
                     DIQPQEDGSFEIYTAWEDLGSVKDGIEAAGFKIENAEVTMIPSTTVELDAETAPKLLD
                     LINRLEDCDDVQNVYHNGEISDEVAALL"
     gene            1361..>1842
                     /locus_tag="PXE44_002524"
     CDS             1361..>1842
                     /locus_tag="PXE44_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589977.1"
                     /translation="MKLVKFVPALAATLVLAACSNANTQSAPQQAQDAVTKVAEQATQ
                     QVNEIAGNAPRASGAVKVEKATDGAKSIVYRCQSGKRVTANYVFQGEEAKAVNLLVQS
                     GGKTQKIPTLTRDASNQDSTSFKTDKYSWDLDTGFTLSSNGQSGMLTKYGAKTDTIVA
                     "
BASE COUNT          609 a          356 c          391 g          486 t
ORIGIN      
        1 tacctaaaaa aattaaattt cacatcttag taataaaatg aactataaaa atcctcatag
       61 tatccttgtg gtgatttatg ctaaaaatac ccaacgagta ttgatgctac aacgcaagga
      121 cgatcctgat ttttggcaat cggtgaccgg ttcgatcaat attggagaaa caccgctgga
      181 agcagcagaa cgggaagtga gcgaagaaac cggtttgcaa acttttgagc aaaaacaacc
      241 gcttgttgat tgccaaaagc agatagaatt tgaaattttc ccgcattttc ggtataaata
      301 cgcacctaat atcacacatt gtgtcgaaca ttggtttttg ctgccacttg atagtgagca
      361 agagcctatt ttaaccgaac atttggcata tcgttgggtt tcagtggaag atgcggttcg
      421 actgactaaa tctcccaaca atgcccaagc aatacaagaa tatttaacat caaaataagg
      481 aaacaacaga atggcaggcc atagtaagtg ggcaaatatt aaacaccgta aagcggcaca
      541 agatgcacaa cgcggtaaaa tttttactaa attaattcgt gaattggtga ctgccgccaa
      601 actcggcggt ggtgatacct cttccaaccc tcgtttgcgt gcggcggtgg ataaagcgtt
      661 atccagcaat atgacccgtg ataccatcaa ccgtgcaatc gagcgtggtg taggcggtgg
      721 cgatgatacc aatatggaaa ccaaaatcta cgaaggttac ggcccgggtg gtacagcagt
      781 gatggtggaa tgtttaagtg ataacgctaa ccgtacgatt tcccaagttc gtccaagttt
      841 cacaaaatgt ggcggtaacc taggtactga gggttcggta ggttatttat ttagcaaaaa
      901 aggcttaatt ttagtggcaa atgccgatga agatgcctta actgaagctg ctattgaggc
      961 cggtgcggac gatattcaac cacaagaaga tggtagcttt gaaatttata ccgcttggga
     1021 agatttaggc tcagtgaaag acggcattga ggctgcaggc tttaaaattg agaatgcaga
     1081 agtaacaatg attccatcaa ctaccgttga acttgacgca gaaactgcac caaaacttct
     1141 tgatttaatc aaccgccttg aagattgtga tgatgtacaa aacgtttatc acaatggtga
     1201 aatcagtgac gaagtcgctg cattactgta ataaaaaaat cacgcaaaat aagcataatt
     1261 taggttatgc ttatttttta tgaacttttt tcctatatta ctgactaacc taatgtagtt
     1321 tatatgctac tactttacta taacatttta aggagagtct atgaaattag tgaaatttgt
     1381 ccctgcccta gcagcaactt tagtattagc cgcttgctct aatgcaaaca cacaatctgc
     1441 tcctcagcaa gctcaggacg ctgtgactaa agtggctgaa caagcaacac agcaagtgaa
     1501 tgaaattgca ggtaatgcac ctcgtgcaag tggtgcagta aaagtagaaa aagcaactga
     1561 tggtgcaaaa tcaattgtat atcgttgcca aagtggtaaa cgtgtaactg cgaattatgt
     1621 cttccaagga gaagaggcaa aagcagtaaa tctattagtc caaagcggcg gtaaaacaca
     1681 aaagatccca acattaactc gtgatgcctc aaaccaagat tctacttcat tcaaaacgga
     1741 taaatattct tgggatttag acacaggctt taccttatca tctaatggcc agtcaggtat
     1801 gctaaccaag tatggtgcta aaacagatac catcgttgct aa
//
LOCUS       DANLZC010000231         1739 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.233, whole genome shotgun sequence.
ACCESSION   DANLZC010000231 DANLZC010000000
VERSION     DANLZC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.233"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(25..1564)
                     /locus_tag="PXE44_002525"
     rRNA            complement(25..1564)
                     /locus_tag="PXE44_002525"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          408 a          523 c          347 g          461 t
ORIGIN      
        1 acttgctgtc gttctcgatt ttggtaagga ggtgatccaa ccgcaggttc ccctacggtt
       61 accttgttac gacttcaccc cagtcatgaa tcataccgtg gtaaacgccc ccctttcggt
      121 taagctatct acttctggta caacccactc ccatggtgtg acgggcggtg tgtacaaggc
      181 ccgggaacgt attcaccgca acattctgat ttgcgattac tagcgattcc gacttcatgg
      241 agtcgagttg cagactccaa tccggactta gacgtacttt ctgagattcg ctccccatcg
      301 ctggctcgcc gccctctgta tacgccattg tagcacgtgt gtagccctac tcgtaagggc
      361 catgatgact tgacgtcatc cccaccttcc tccggtttat caccggcagt ctcctttgag
      421 ttcccgacct aatcgctggc aacaaaggat aagggttgcg ctcgttgcgg gacttaaccc
      481 aacatttcac aacacgagct gacgacagcc atgcagcacc tgtctcatgg ttcccgaagg
      541 cactctcata tctctacaag attccatgga tgtcaagagt aggtaaggtt cttcgcgttg
      601 catcgaatta aaccacatgc tccaccgctt gtgcgggccc ccgtcaattc atttgagttt
      661 taaccttgcg gccgtactcc ccaggcggtc gatttatcac gttagctacg ggcaccagag
      721 ttaaacccca atccccaaat cgacagcgtt tacggcgtgg actaccaggg tatctaatcc
      781 tgtttgctcc ccacgctttc gcacatgagc gtcagtacat tcccaagggg ctgccttcgc
      841 cttcggtatt cctccacatc tctacgcatt tcaccgctac acgtggaatt ctacccctcc
      901 ctaaagtact ctagttgtcc agtctgaaat gcaattccca ggttaagccc ggggctttca
      961 cacctcactt aaacaaccgc ctgcgtgccc tttacgccca gttattccga ttaacgctcg
     1021 caccccccgt attaccgcgg ctgctggcac ggagttagcc ggtgcttctt ctgtgattaa
     1081 cgtcaatcga ctgttctatt aaaacaatcg ccttcctcgt caccgaaaga actttacaac
     1141 ccgaaggcct tcttcattca cgcggcatgg ctgcatcagg gttcccccca ttgtgcaata
     1201 ttccccactg ctgcctcccg taggagtctg gaccgtgtct cagttccagt gtggctggtc
     1261 atcctctcag accagctaga gatcgcgggc ttggtgagcc tttacctcac caactaccta
     1321 atcccacttg ggctcatctt atggcaggtg gccaaatggt cccacccttt agtcctaaga
     1381 cattacgcgg tattagctac cgtttccagt agttatcccc ctccataagc cagattccca
     1441 agcattactc acccgtccgc cactcgtcac ccaaggagca aactcctatg tgctaccgtt
     1501 cgacttgcat gtgttaagcc tgccgccagc gttcaatctg agccatgatc aaactcttca
     1561 attcaaaaag tttaatcgct caatactgct gacataaaat cgcacttcca aatttaaacc
     1621 taattaaaat taagttaaaa gaaagtaatg aatttctagt tagcacctat taagacttca
     1681 aaattaaaaa aatgtttttc aatcaagtca atcaacaagt gcccacacag attgtctga
//
LOCUS       DANLZC010000232         1642 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.234, whole genome shotgun sequence.
ACCESSION   DANLZC010000232 DANLZC010000000
VERSION     DANLZC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.234"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..1642
                     /locus_tag="PXE44_002526"
     CDS             <1..1642
                     /locus_tag="PXE44_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL5589978.1"
                     /translation="DLNADTSQAFLNELDQTLWTAADKLRKNLDAANYKHIVLGFIFL
                     KYISDSFTDFQAKLKTQLTTPESELYLDPALFDEQEFSQILAEELEQRDYYAAENIFW
                     VPEQARWDNIKSLSKLNLGDELPWGDKFKGVSRLIDDAFEAIERENPKLKGVLQRIAG
                     FGVPDEMLTGLIDLFSRTNFTQPMHNGEPVHLQAKDILGHVYEYFLGQFALAEGKKGG
                     QYFTPKSIVTLIVEMLEPYSGRIYDPAMGSGGFFVQADRFIQAHAGNRNAISVYGQES
                     NSTTRKLAVMNMAIRGIPFDFGDKPEDTLLNPLHIDKKMDVVMANPPFNQKEWWNESL
                     ANDPRWAYGTPPQGNANFAWLQHMIYHLSPKGKMALLLANGSMSSQTSGEGDIRKNIV
                     QADLVEAMIALPNQLFTNTQIPACIWIINKAKARKGEVLFINATQIGYLKDRVLRDFT
                     ADDIAKISDTYHNWQKQNGYENIPAFCYCATLDEIAKNDFVLTAGRYVGAVQEENDGV
                     PFAEKMQELTALLNEQFKQGRELEQQIAENLKGLGYGI"
BASE COUNT          485 a          436 c          356 g          365 t
ORIGIN      
        1 tgatctcaac gctgacacaa gtcaagcctt tttaaacgaa ctcgaccaaa ccctctggac
       61 tgccgccgac aaactgcgta aaaacctcga tgccgccaac tacaaacaca tcgttcttgg
      121 ctttatcttc ctaaaataca tctccgacag ctttaccgat ttccaagcca agctaaaaac
      181 ccagcttacc acccccgaaa gcgaactcta tcttgaccct gcactatttg acgaacaaga
      241 atttagccaa attcttgccg aagagttgga acagagagat tactacgccg ctgaaaacat
      301 cttttgggtg ccggagcaag cccgctggga caacatcaaa tcattaagca aactcaatct
      361 tggcgatgaa ttgccttggg gagacaaatt taaaggtgtc agccgcttga ttgatgatgc
      421 ctttgaagcc atcgaacggg aaaaccccaa actcaaaggc gtactccaac gcattgccgg
      481 ctttggcgtg cctgatgaaa tgctcacagg cttaattgac ctgttctcac gcaccaattt
      541 cacccagcct atgcacaatg gcgaacctgt gcatctgcaa gccaaagaca ttttagggca
      601 cgtctatgaa tactttcttg ggcaatttgc ccttgccgaa ggcaaaaaag gcggtcaata
      661 cttcacgcca aaatccatcg ttaccctgat tgttgaaatg ctcgaaccct attcagggcg
      721 gatttacgac ccagctatgg gcagcggcgg cttttttgtg caagctgacc gctttattca
      781 ggctcacgca ggcaaccgca acgccatttc cgtttatggg caagaatcca actccaccac
      841 tcgcaaactg gcggtgatga atatggcgat tcgtggtatt ccctttgact ttggcgacaa
      901 gcccgaagat accctactaa accctttgca catcgacaaa aaaatggatg ttgtgatggc
      961 aaatccgccc tttaaccaaa aagagtggtg gaatgaaagc ctagcaaacg atccacgctg
     1021 ggcatacggc acaccgccgc aaggcaacgc caactttgcg tggttgcaac atatgattta
     1081 ccacctctcc cccaaaggca aaatggcact cctgctcgcc aacggctcaa tgagcagcca
     1141 aacttcaggc gaaggcgaca ttcgcaaaaa catcgtgcaa gctgaccttg tcgaagcgat
     1201 gattgccctg cccaatcagc tattcaccaa cacccaaatc cctgcctgca tttggattat
     1261 caataaagcc aaagccagaa aaggtgaagt gctgtttatc aacgccaccc aaataggcta
     1321 cctgaaggac cgcgtcttgc gtgattttac cgctgatgac atcgccaaaa tcagcgacac
     1381 ctaccacaac tggcaaaaac agaacggcta cgaaaatatc cctgcgtttt gttattgtgc
     1441 cacgctggac gaaatcgcca aaaacgattt tgtgctgacc gcagggcgat atgtcggtgc
     1501 ggtacaagaa gaaaatgacg gcgtgccgtt tgcagaaaaa atgcaggaat tgaccgcttt
     1561 attgaatgaa caatttaaac aagggcggga attggaacag caaatcgcag agaatttaaa
     1621 ggggttggga tatggcattt aa
//
LOCUS       DANLZC010000233         1590 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.235, whole genome shotgun sequence.
ACCESSION   DANLZC010000233 DANLZC010000000
VERSION     DANLZC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.235"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..>1590)
                     /locus_tag="PXE44_002527"
     CDS             complement(<1..>1590)
                     /locus_tag="PXE44_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589979.1"
                     /translation="EIWTAFIALESDQCPAIERLLTWCQKSSGQENIEPEALLLGAIA
                     VSWLFTSTDKRSRNGYINALAHLLWNRLEIALALFKEFIEVNDPYVMEGILSAIHGAV
                     AYSENSSGLKALALEIDNQIFNQPETEEIYPNVLVRDYARHIIEYSLQKDEYSSDDIE
                     IIRERITPPYRSSFPQNLPTNEEIDKKYHSDGQRIIIRSMTTEYGRGMCGYGDFGRYT
                     FEAKFRNWESYKFGNLINVDLLSNYACQMIFEQFGYDDKKHLEFDKETRSRYWRRDDT
                     NKIERIGKKYQWLALYEILARVMDNFPTFSNRYADEIGLEYITNLDNFYLPKTQIKIV
                     PPTKTDVFFQYINQKQCKTKHYFTIQNDELIDDWVKINDFPDVSNLLEVTINDEQWLV
                     LERHDDFRELAKFGQKETYDRQMWLQIRSYLVKESQFSKAKNWLAQQDFMERWMPEGN
                     EHYGMNIHQFFAENTLNEWQEIYRHRENKSPSFKVIPSVDSHTWESTYGNDGISFYAP
                     IPMLYHGMKMKNAIKDGYWVDL"
BASE COUNT          508 a          299 c          223 g          560 t
ORIGIN      
        1 taaatcaacc caataaccat ctttaatcgc atttttcatt ttcattccgt gataaagcat
       61 aggaattggc gcataaaatg aaatgccgtc attgccataa gtagattccc aagtgtgaga
      121 atcgacacta ggtattacct taaatgaagg cgatttattt tccctatgtc gataaatttc
      181 ttgccactca tttaatgtat tttcggcaaa aaattgatga atattcattc catagtgttc
      241 attaccttct ggcatccatc tttccataaa atcttgttgt gccaaccaat tttttgcttt
      301 tgaaaattga gattctttaa ctaaataact tctaatttgt aaccacattt gcctatcata
      361 agtttctttt tgcccaaatt ttgctaattc acgaaaatca tcgtgtcttt ctagaacaag
      421 ccactgttca tcattaatcg ttacttccaa aagattacta acatcaggaa aatcatttat
      481 ttttacccaa tcatcaatta attcatcatt ttgaatggta aaatagtgtt ttgttttaca
      541 ttgtttttga tttatgtatt gaaaaaatac atctgtttta gttggcggta caattttaat
      601 ttgggttttg ggtagataaa agttgtccaa attagtaata tattcaagcc ctatttcatc
      661 ggcatagcga ttagaaaaag taggaaaatt atccatcacc cttgccaaga tttcatacaa
      721 agccaaccat tgatatttct tgccaattcg ctcaatttta ttagtatcat ctcttcgcca
      781 atatcttgaa cgagtttctt tatcaaactc aaggtgcttc ttgtcatcat agccaaattg
      841 ctcaaaaatc atttggcaag cataattact taataaatca acattgatga gattgccgaa
      901 tttataactc tcccaatttc ggaatttggc ttcaaaagta tatctgccaa aatcaccata
      961 tccacacata ccacgaccat attcagttgt cattgaacgg ataatgattc tttgtccatc
     1021 agaatgatat tttttatcaa tttcttcatt ggttggtaag ttttggggaa atgaacttct
     1081 ataaggtggc gtaattcttt ctctgattat ctcaatatca tctgatgaat attcatcttt
     1141 ttgcaaagag tattcaataa tatggcgagc ataatcacga accaaaacat tgggataaat
     1201 ttcttctgtt tcaggttgat taaatatttg attatctatc tctaatgcaa gtgcttttaa
     1261 gccagatgaa ttttcagaat aagcaacagc tccgtgaatg gcagataaaa taccttccat
     1321 aacataaggg tcattaacct ctataaattc cttgaataac gccaatgcaa tttccaatct
     1381 attccataat aaatgagcca gtgcattaat atagccattt ctacttcgtt tatcggtaga
     1441 agtaaatagc caactaacag caattgcacc caataatagt gcttcgggtt caatattttc
     1501 ttgaccactt gatttctgac accaagttag taatcgttca attgctgggc attgatcaga
     1561 ctccaaagca ataaaagctg tccaaatctc
//
LOCUS       DANLZC010000234         1587 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.236, whole genome shotgun sequence.
ACCESSION   DANLZC010000234 DANLZC010000000
VERSION     DANLZC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.236"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            322..576
                     /locus_tag="PXE44_002528"
     CDS             322..576
                     /locus_tag="PXE44_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589980.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFCNYQQFMPEVEAKESLR
                     KQEPELDDRYNEEEYSEEEYPDENDASDENDNYDETYNVF"
     gene            776..952
                     /locus_tag="PXE44_002529"
     CDS             776..952
                     /locus_tag="PXE44_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589981.1"
                     /translation="MATSSFDKRFVVKDPKAVKVLIEALQSPIKVVLPKKDLEKEEKE
                     ALCAIKKRLASMGL"
     gene            913..1464
                     /locus_tag="PXE44_002530"
     CDS             913..1464
                     /locus_tag="PXE44_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589982.1"
                     /translation="MCNQKTLSLYGLVKELGIEKTQLFLEQAFSCSRNKDVESFLLNK
                     AIRFETALAAATHLIINEQGDILAYFTLSFKEIEVEVSKSQLKKLTAGLSNDSHLRVL
                     LIGQIGKNEKVIDNPAKLPDILNHIYAQLYKAQRVLGGRVVILECENTPKLLELYKQN
                     KFQILNTIGSENGLKTLFFIPDF"
BASE COUNT          586 a          273 c          266 g          462 t
ORIGIN      
        1 ccgctactca acatacaggg atggttatct tgataacgct cacgatactc ttgagcaata
       61 gctagtaatt gctgattagt catataaagt ctccaataac ataataaacc tgaaaacgtt
      121 cttagtattc tggcaacatt tttataaata gaaaccaaaa actatattca aatcattttt
      181 agtttctgct caacattcct atgtttttcc atacactgaa gtaaatttac ctaaccctat
      241 tttgctgtga aaccaaaact cctcacgctt atccttagta gcatttcgat gtcgctcact
      301 tatgccaccc ctcaattagg gatgatatgc cacgctgatc ctgaatatgt aaaatacaac
      361 tacgccggac tttgggaaaa aggcagcgat ggcacttttt tctgcaatta ccagcaattt
      421 atgcctgaag ttgaagctaa agaatcacta aggaaacaag aaccagaact tgatgataga
      481 tataacgaag aggaatatag cgaagaagaa tatcctgatg aaaatgatgc cagcgatgaa
      541 aacgataact acgatgaaac ttataatgtt ttctagtatg tatatggaaa ccggaggaat
      601 ccgtgattga aaaggcttgc atttccaaat atagtctata tattgtggta attgaaagta
      661 tttctatgct aacaagcata aggctatgta atcgaaaacc acccacaagg cctaaataca
      721 gtattattac agccatgagt aaccatggta atttaaatat ttaaggagtt ctataatggc
      781 aacatcatca tttgataaac gctttgtagt caaagatcca aaagctgtca aggtattaat
      841 tgaggcatta caaagcccaa tcaaagtagt tttacctaaa aaagacttag aaaaagaaga
      901 gaaagaagca ctatgtgcaa tcaaaaaacg cttagcctct atgggcttgt aaaagaacta
      961 ggtattgaaa aaacacaact ctttttggag caagcatttt cttgcagtag aaataaagat
     1021 gttgaaagtt ttctacttaa taaagcaatc agatttgaaa ccgcattagc agcagcaaca
     1081 catttaatca ttaatgaaca gggcgatatt ttggcttact ttaccctctc ttttaaagaa
     1141 attgaggtgg aagtaagcaa aagccaatta aaaaagctaa ctgctggttt aagtaatgat
     1201 tcacatttaa gagtattgct tatcggtcaa atcggcaaaa atgaaaaagt aattgacaat
     1261 ccagccaaat tgccagatat tctaaatcat atctatgcac aactttataa agcacaacga
     1321 gttttaggtg ggcgagtcgt tatattagaa tgtgaaaata cgcctaaatt attagaacta
     1381 tataaacaaa ataaatttca aattctcaat accattggaa gtgagaatgg tctaaaaaca
     1441 cttttcttta ttcctgattt ttaactacag ctaatatttc attagaagaa agtcgccagc
     1501 tttgtcagga agtcatatat gggagaaaga acaatgtctc tacttaaaaa agcacaaacg
     1561 tttatttcta tttttaatga cgtgcga
//
LOCUS       DANLZC010000235         1576 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.237, whole genome shotgun sequence.
ACCESSION   DANLZC010000235 DANLZC010000000
VERSION     DANLZC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.237"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            134..820
                     /locus_tag="PXE44_002531"
     CDS             134..820
                     /locus_tag="PXE44_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589983.1"
                     /translation="MAANSINASHIVGSSITADKLNVNNLAAISANLGKVTAGTITGT
                     TISGGTISGTTITGGIIKGARIEGLTGKFTGELEINQLIGGNIYEAKTWTSKATGKSK
                     TYSETKNSFALSDTYYEYVCQFVLPATKATRTLYVVHTPTGESKSETFYGSGSNRTPT
                     TITLPSVIDISNTISPNVSNPFNDSGAILIANTQYVVTCRAWCAGRGRSAKVNFRAII
                     SSTNGSSYQL"
     gene            830..988
                     /locus_tag="PXE44_002532"
     CDS             830..988
                     /locus_tag="PXE44_002532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589984.1"
                     /translation="MKKIILLTTIMVSACTTQYKQDYTTKPTFCYQLAPQEQLPGKNC
                     IGSGGHSG"
     gene            complement(997..1296)
                     /locus_tag="PXE44_002533"
     CDS             complement(997..1296)
                     /locus_tag="PXE44_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5589985.1"
                     /translation="MKTLDQMLNELSPERRQKVEDMAQEMIREIQLSKLREELNLSQQ
                     QLATALGISQPAIAKLEQKDNDPRLSTLKRYIEAMGGKLKLAVELPTGDERVIRI"
     gene            complement(1268..>1576)
                     /locus_tag="PXE44_002534"
     CDS             complement(1268..>1576)
                     /locus_tag="PXE44_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5589986.1"
                     /translation="ERVLACLIYLRKFGYSLGRPYADTVYQSKFPNMKELRIQNKGKP
                     IRAFYAFDPLRQAIVLCAGEKGKDKRFYEKMIALADAEFTSYLNNLEKENENVRSNAQ
                     "
BASE COUNT          511 a          293 c          313 g          459 t
ORIGIN      
        1 cccttggcaa aatgccggtg taaccgctat ccacggtggc tcaatagtta ctaataccgt
       61 tactgctcaa caaattgcag caaataccat taccgccaac gaaatcgcag ccggcacgat
      121 tgcagctcga aatatggcag caaactcgat taatgcaagt catattgtgg ggagttcgat
      181 cactgcggat aagctaaatg taaacaattt agcggcaata tcggcaaatc ttggtaaggt
      241 aactgctggt acaattacag ggacgacaat tagtggtggg acaatttcag gtacaacaat
      301 tacaggggga ataattaaag gagcaagaat tgagggatta acaggtaaat ttactggaga
      361 acttgaaatc aaccaattga tcggaggtaa tatctatgag gctaaaactt ggacatcaaa
      421 agcaacaggg aaaagtaaaa catattcaga aacgaaaaac agttttgcat taagtgatac
      481 atattatgag tatgtatgtc aatttgtact acctgctaca aaagcgacga gaacattgta
      541 tgttgtccac actccaacag gagagagtaa aagcgaaact ttttatggta gtggtagtaa
      601 tcggacaccg acaacaatta cattaccgtc tgtaattgat attagcaata ctatttcacc
      661 taatgtatct aacccattta atgattctgg ggcaatactc attgctaata ctcaatatgt
      721 agtaacgtgt cgtgcgtggt gtgcagggcg tgggagaagt gctaaagtga attttcgagc
      781 aattatatca tctacaaatg gttcatctta tcaattatag gaggcaaata tgaaaaaaat
      841 tatactactt acaacaatta tggtttcagc ttgtacaact caatataagc aagactatac
      901 aacaaaacca accttttgtt atcaactagc ccctcaagag caattaccgg ggaaaaactg
      961 tattggctca ggagggcatt caggttaaca aagtaattaa atcctaatta cacgctcatc
     1021 gcctgtaggc aattcaacgg caagttttaa cttaccgccc attgcttcaa tatagcgttt
     1081 gagcgtggat aatcttggat cgttatcttt ctgttcaagt ttagcaatag caggctgtga
     1141 aataccgaga gctgtagcta attgttgttg agagagattt agctcttctc ttagttttga
     1201 aagttgtatc tctctaatca tttcttgagc catatcttcc actttttggc ggcgttcagg
     1261 agagagttca ttgagcattt gatctaacgt tttcattttc tttctctaaa ttatttaaat
     1321 aggaggtaaa ttccgcatca gcaagagcga tcattttttc atagaatctt ttgtctttac
     1381 ctttctcgcc agcacaaagt actatcgctt ggcgtaaagg gtcaaaagca taaaatgctc
     1441 gaattggctt acctttgttt tgaatgcgta actctttcat attagggaat ttagactgat
     1501 aaactgtatc agcataaggt ctgcctagac tataaccgaa tttccgcaga tagattaaac
     1561 aagccaagac acgttc
//
LOCUS       DANLZC010000236         1526 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.238, whole genome shotgun sequence.
ACCESSION   DANLZC010000236 DANLZC010000000
VERSION     DANLZC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.238"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..216
                     /locus_tag="PXE44_002535"
     CDS             <1..216
                     /locus_tag="PXE44_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589987.1"
                     /translation="KNLTDESEEQAILAYLTENIMARIEKEQPLEKTLILYSEQLASF
                     EQKSPMALHREKFEAKPTGLAELINNL"
     gene            227..1177
                     /locus_tag="PXE44_002536"
     CDS             227..1177
                     /locus_tag="PXE44_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HDL5589988.1"
                     /translation="MSLSTFAHALKLDKQGNVILFDDSPEEQAKLISRLKQNRLFKLL
                     NLVQTKHAKGEAIGLFTPIAGTTNTESGEEREPKQALAPAQKYHCEQINIDSYIRYEK
                     IDAYNLENSLEERLNQLLDDNLLRGLLMVGWQGVSRADTSNATNNQLAQDVKKGWLQK
                     IREGKPEAVINGATIGKEQQYKSLNALIKQGLGKISPAYAMGGDMIAICGRDIIADDV
                     IQLEHNDLSENLADLLTLCQQAKGGLKAVSIPYFPSNAILITRLDNLSLYLHLNNIRR
                     KAFDTPQKDSVETFYSLNVDYIVEDFNACCLIENIEVIEE"
     gene            1174..1368
                     /locus_tag="PXE44_002537"
     CDS             1174..1368
                     /locus_tag="PXE44_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589989.1"
                     /translation="MSLESYIQQAEEMELLEKLDFVSDLLELEKVSEIEAYRILTAHG
                     EPKPQPAQEKLIGCVDFLDD"
BASE COUNT          563 a          317 c          291 g          355 t
ORIGIN      
        1 aaaaacctca cagatgaaag tgaagagcaa gctattctgg catatctaac agaaaatata
       61 atggctagaa ttgaaaagga acagccactt gaaaaaacat taattcttta ttctgaacag
      121 ttagcaagtt ttgaacaaaa atcgccaatg gcattacacc gtgaaaaatt tgaagcaaaa
      181 cctaccggct tagctgaatt aattaataac ttataagagg taaaaaatga gtttatccac
      241 tttcgcccac gcattaaaac tagataaaca aggtaacgtt attttatttg acgatagccc
      301 cgaagaacag gcaaaactaa ttagccgctt aaaacaaaat cgccttttta aattattgaa
      361 tttagtgcaa accaagcacg caaagggcga agcgattggg ctattcacac cgatagccgg
      421 cacaaccaac accgaaagcg gagaggaacg agagccaaaa caagcccttg cacccgctca
      481 aaaataccac tgcgaacaaa tcaacattga tagctatatt cgctatgaaa aaatcgatgc
      541 ttacaacctc gaaaacagct tagaagaacg cttaaatcag ttacttgatg acaaccttct
      601 aagagggctt ttaatggttg gctggcaagg cgtaagccgt gcagatacat caaacgcgac
      661 aaacaaccaa ctcgcccaag acgtgaaaaa aggctggcta caaaaaatca gagagggcaa
      721 gcccgaagcg gtaattaatg gagcaaccat aggcaaagag caacaataca aaagcctaaa
      781 tgctctgatt aagcagggat taggcaaaat tagcccagcg tatgcaatgg gcggagatat
      841 gatcgccatt tgtgggcggg atattatcgc tgatgatgta attcagcttg aacacaacga
      901 tttaagcgaa aacctagccg acttattaac cttatgccaa caagctaaag gcggactaaa
      961 agcggtaagt attccctact tcccaagcaa tgcgatttta atcactcgat tagataacct
     1021 ttcgctatat cttcatttaa acaatatccg ccgtaaagcg tttgatacgc cacaaaaaga
     1081 cagcgtagag acattttact ccctcaatgt ggattacatt gtagaagatt ttaacgcctg
     1141 ttgtttaatt gaaaatattg aggttattga agaatgagcc tagaaagcta tatccaacaa
     1201 gcggaagaaa tggagctttt agaaaaactc gattttgttt cagacctgtt agaacttgag
     1261 aaagtcagcg aaattgaagc ctatcgcatt ttaaccgccc acggcgaacc taaaccccaa
     1321 ccggcacaag aaaagctaat aggttgcgta gattttcttg atgactaacc caagccccga
     1381 aaggggcttt ttgcattttt gcaacaaaaa acgaccgctt gcaccctttg aaattattgc
     1441 tatcattaat aaaaaacaat aaccaaatca aatgagagta cagcaatgac aaactataaa
     1501 ctaccggcta gaaagtggta cacctt
//
LOCUS       DANLZC010000237         1501 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.239, whole genome shotgun sequence.
ACCESSION   DANLZC010000237 DANLZC010000000
VERSION     DANLZC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.239"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            3..317
                     /locus_tag="PXE44_002538"
     CDS             3..317
                     /locus_tag="PXE44_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589990.1"
                     /translation="MDLNKVYIKNDGTTFQKLSVLSSIDFLINPEITLSSGAIYLNLS
                     FDKALRTKTRVFALTLINDNHLFIPQSLLNLRTMNDFPKGNKIIGIKSVHLEDKTEII
                     YL"
     gene            complement(1049..1486)
                     /locus_tag="PXE44_002539"
     CDS             complement(1049..1486)
                     /locus_tag="PXE44_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589991.1"
                     /translation="MKEWYTLKELLDVGGLPSTPQGIILYAKKDKWRRRRVSGVKGNV
                     FEYYVYDMPESVQTSLGVTKETLALKQELIDDIQKENEKFLNQILSDIKNVLLNSGVL
                     RIRERLSITKEEEILLSYYRNSSEQDKTTILHMVERLSRASKN"
BASE COUNT          508 a          250 c          199 g          544 t
ORIGIN      
        1 aaatagacct aaataaggtt tatataaaaa atgatggcac aacattccaa aagttgtctg
       61 ttttaagttc tatagatttt ttaattaatc cagagataac tttgtcatca ggtgcaattt
      121 atttaaacct ttcttttgat aaagctctta gaactaaaac cagagtcttt gctctcacat
      181 tgataaatga caatcactta tttattccac aatctttact gaatcttagg accatgaatg
      241 atttccctaa gggtaacaaa attattggta taaaatcagt tcatttagaa gataaaacag
      301 aaatcatcta cttatagaaa aaagaataag agaaattata ttctcccatt tttattttat
      361 cagagtaaat tcacaatctt gggcatttgc attatcaagg caatactcaa aggagctcaa
      421 tctttgataa atcatcaacg gagaagtcaa ttaaggcact tgcaagaaca tttaaggata
      481 gtgatcatac ttatgggaag ctcaggaact tctcagaagc tccgctgttt cttgactcaa
      541 agtcatcaag attaatttat tagctgattt ggtttcctat gccatattta ggcattatga
      601 acataatgat agctcatttt acgatcttat aaagaataac tttgatcatc ataatggacg
      661 tattcacggt ttatatttac gaaatacaga ggatataatc ccataagtat gaagtataga
      721 agggcgatat cctctctaat aataaactct ctcaacattt ctatgtcata ctcaatatat
      781 cagagcttaa tactaatatc attatgcatt gacaatttct gaatgacata gatattttcc
      841 ggtttcttca ttaattgagt aacagcggta tatcaaaatc tatatagtac ctaagaaagg
      901 cttacttgct tagaaatata ttaaaaaata tcttttagct tattttgaag catatcttac
      961 tatcttatga atacataggg gaaaatattc taaatttatt ctagcgatat acagagcttt
     1021 atcattaata gtattaaggg atttacatct aatttttaga tgctctagat agtctttcta
     1081 ccatatgaag aattgtcgtt ttatcttgtt ctgagctatt tctataatag cttaataaaa
     1141 tttcttcttc tttagtaatg cttaatcttt ctctaattct caatacccca ctattgagca
     1201 aaacattttt tatatcagaa agtatttgat ttagaaattt ttcattttct ttttgaatat
     1261 catctatcaa ttcctgtttc aatgccaaag tttccttagt tactcctaaa ctagtttgta
     1321 cagattctgg catatcatac acgtagtatt caaaaacatt acctttcact ccagagacac
     1381 gccgtctacg ccatttatct tttttagcat ataagattat accttgtggt gttgaaggga
     1441 gccctcctac atctaaaagc tctttaagag tataccattc tttcatatta cctcattgac
     1501 t
//
LOCUS       DANLZC010000238         1454 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.240, whole genome shotgun sequence.
ACCESSION   DANLZC010000238 DANLZC010000000
VERSION     DANLZC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.240"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            13..1293
                     /locus_tag="PXE44_002540"
     CDS             13..1293
                     /locus_tag="PXE44_002540"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005762304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDL5589992.1"
                     /translation="MSKATLTLETGEKLELNMQKGTLGYQTIDIQPFLKHKLFAYDPG
                     LVSTAVCRSNITYVDGDEGVLLYRGYPIDQLAKHSDYLEVGYMLLFGERPTKEEYKDF
                     VQLIKKHTLVHEQLTKFFSGFRRDSHPMAVMCGVSGALAAFYHDAIDVNNQAHRELTA
                     IRLLAKIPTLAAMCYKYSIGQPFMFPQNHLSYAGNFLYMMFATPCEPYEVNPVLERAL
                     DRIFILHADHEQNASTSTVRTAASSGANPFACIAAGIASLWGPAHGGANEACINMLKE
                     IGTVDRIPEFIARAKDKNDPFRLMGFGHRVYKNYDPRAKVMRETCHEVLKELNIDNPL
                     FEVALELERIALSDPYFIEHKLYPNVDFYSGIVLKAIGIPTSMFTVMFALARTVGWIA
                     HWKEMYLQGNIKIVRPRQIYTGHTKRDFEPMDKS"
BASE COUNT          429 a          337 c          281 g          407 t
ORIGIN      
        1 taggagtaag ctatgtcaaa agcaacatta acactcgaaa caggtgaaaa acttgaactc
       61 aatatgcaaa aaggaacctt agggtatcaa acgattgata ttcagccttt cttaaagcat
      121 aagttatttg cttatgatcc cggattggtt tcgaccgctg tatgccgatc caatatcact
      181 tatgttgatg gcgatgaagg ggttttactc tatcgaggct acccgattga ccaattagcc
      241 aaacattctg attacttaga agtcggttat atgctgttat tcggcgaacg ccctaccaaa
      301 gaagaataca aagacttcgt tcaactcatc aaaaaacaca ctcttgtaca cgaacaacta
      361 accaaattct tctccggttt ccgccgagat tcgcacccaa tggcggtgat gtgtggggta
      421 agtggagctt tggctgcctt ctaccacgac gccattgatg taaacaacca agcccaccgt
      481 gaattaaccg ccattcgttt gttagccaaa atcccaaccc ttgcggcgat gtgttataaa
      541 tactctatcg gtcagccgtt tatgttccca caaaaccact tatcttacgc cggcaacttc
      601 ttatatatga tgtttgccac tccttgtgaa ccgtacgaag tgaaccctgt gttagagcgt
      661 gcgttagacc gaattttcat tttacatgca gaccacgaac aaaacgcttc gacctcaacc
      721 gtgcgaaccg ccgcttcatc aggtgcaaac ccatttgctt gcattgctgc cggtattgcc
      781 tctctctggg gacctgcaca cggaggagca aatgaagcct gtatcaatat gttgaaagaa
      841 atcggcactg tggatcgcat tcctgaattt attgctcgag ccaaagataa aaatgacccg
      901 ttccgcttaa tgggctttgg gcatcgtgtc tataaaaact acgaccctcg tgcgaaagta
      961 atgcgtgaaa cctgccacga agtgttaaaa gaactgaaca ttgataaccc actatttgaa
     1021 gtggcattgg aattagaacg tattgcgtta agcgaccctt atttcattga gcataaactt
     1081 tatccaaacg tagatttcta ctccggaatc gtgctaaaag ccatcggtat tccaacctca
     1141 atgtttaccg taatgtttgc cctagccaga accgttggct ggattgctca ctggaaagag
     1201 atgtatctcc aaggcaatat caaaatcgtt cgtccacgtc aaatctacac cggacatact
     1261 aaacgtgatt ttgagccaat ggataaatca taagattggc aaaaaatcga aaaattttaa
     1321 ccgcttgttt ccacaagcgg ttatattttg cataaaattt gcaaatcagg ctaatttatt
     1381 tagcagttgt gttctaacat cttgaccatt ttcatcgctt tttacagcca taataaataa
     1441 gccttttcct tgac
//
LOCUS       DANLZC010000239         1399 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.241, whole genome shotgun sequence.
ACCESSION   DANLZC010000239 DANLZC010000000
VERSION     DANLZC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.241"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            115..1185
                     /locus_tag="PXE44_002541"
     CDS             115..1185
                     /locus_tag="PXE44_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589993.1"
                     /translation="MKFNKKLILTFAATLVLSACGGSGSGGSSSTPNHPKPVLVPKTQ
                     NNLQAQNVPQAQNASQAQNAPQAQNAPQAQNAPQVENAPQAQNAPQVENAPQAEVTPP
                     VPQPQSQKIDGSFDKIGSVKLNKEAQTLELSRFTLVDKLGTPPKFDKVSGKKIIEEKD
                     FLVLNLSDINAEQLSGDFLIRRSDDLFYGYYHDTNGKNLVDAADKFSQYFVVYDEKRV
                     NDNISDKLTATYRKKEGFVYGSNPHTKEFAARISKLGDVEIKFENGQAQGSIKDEKDG
                     NAEIFTIKGDTKQLEITPTESNRIIIAILDQNQKSYTPGMEKAIMETKFIDSKAGNSD
                     QKYLIGEAKSDNWQAIMVSEKK"
BASE COUNT          505 a          261 c          249 g          384 t
ORIGIN      
        1 attttcataa gggaaacgtt tacgtaaaac tcctcagacc actcattctt attttatata
       61 aaaaatgtga tagacttctc gcagtttcgt tttatatatt taaggaataa ctaagtgaaa
      121 ttcaataaaa aattaatttt aacatttgct gcaaccttag ttttaagtgc ttgcggagga
      181 agcggtagcg gaggttcgtc ttcaacaccg aatcacccca aaccagtact agtaccaaaa
      241 acacaaaata atcttcaagc acaaaatgtt cctcaggcac aaaatgcctc tcaggcacaa
      301 aatgcccctc aggcacaaaa tgctcctcag gcacaaaatg ctcctcaggt ggaaaatgct
      361 cctcaggcac aaaatgctcc tcaggtagaa aatgctcctc aagcagaggt tactccgcct
      421 gtaccacagc cacaatcaca aaaaattgac ggttcttttg ataaaattgg ttcagtaaaa
      481 ctcaataaag aggctcaaac tcttgagctt agtagattca ctttggtgga taaattaggc
      541 acaccaccga agtttgataa agtaagcggt aaaaaaatta ttgaagaaaa agattttctc
      601 gtattaaatt tgtctgatat taatgctgaa caactctctg gcgattttct tattcgccgt
      661 agcgatgatc tattctatgg ctactatcac gatacaaatg gcaaaaatct tgtcgatgct
      721 gccgataaat tcagtcaata ttttgtcgtg tatgatgaga aacgggtaaa tgataatatc
      781 tctgataaat taacagcaac ttaccgtaaa aaagaaggct ttgtatatgg ttcaaatcca
      841 catactaaag aatttgccgc acggatcagc aaattggggg atgtagaaat taaatttgaa
      901 aatggtcaag ctcaaggaag tataaaagac gaaaaagatg gaaatgctga gatctttact
      961 attaaaggtg atacaaaaca gttagagatt accccaacgg aaagtaaccg aatcattata
     1021 gcaattttag accaaaatca aaaaagctat actccaggaa tggaaaaagc aattatggaa
     1081 actaagttta ttgattcaaa ggctggtaat tccgaccaaa aatacttaat cggtgaagca
     1141 aaaagcgata actggcaagc aataatggtt agcgagaaaa aataaagtta tcttttgcta
     1201 aaaactgaaa taaaaaggct gagtccgggt aatatcggcc tcagtctttt aaattgtaga
     1261 aaatcatctg tagaagatca aacctaatct gacagtcccc cgttttaaat taccgtgtct
     1321 gtcagattaa tttgagctta aattcttttc cacccaaatc cgttttccat caagtaaggt
     1381 tgccatcggt gttctgcca
//
LOCUS       DANLZC010000240         1377 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.242, whole genome shotgun sequence.
ACCESSION   DANLZC010000240 DANLZC010000000
VERSION     DANLZC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.242"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(164..295)
                     /locus_tag="PXE44_002542"
                     /pseudo
     CDS             complement(164..295)
                     /locus_tag="PXE44_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(316..516)
                     /locus_tag="PXE44_002543"
     CDS             complement(316..516)
                     /locus_tag="PXE44_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5589994.1"
                     /translation="MNLTSYLSDRPRGFKADFAKKLGISKSYLRQVETGYSPMPAYLA
                     KKIEEVTNGEVAKSELRPDLWD"
     gene            647..1330
                     /locus_tag="PXE44_002544"
     CDS             647..1330
                     /locus_tag="PXE44_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5589995.1"
                     /translation="MIKGMTLPEIRRKKLTEWFNTRDIPEKEKSYISQLKSGKSSFGE
                     RAARRLENEYGMPSFYLDYDEETESAIVSAMSIQETQSHQYPIHLIDFKAKAGETGFV
                     NTNYPEIIQSIYFSLDGLLEIVGRKSSNGIEMITIPTDSMSPTINKGDVVFIDTTINY
                     YDNEGVYIFVIDDEVYIKRLQKIPGGIYRALSDNKTYEPFDIKRETLDTAIILGKFIR
                     VLPINPKDL"
BASE COUNT          463 a          242 c          257 g          415 t
ORIGIN      
        1 ctattgagcc accgtgaata gcggttacac cggcattttg ccaagggcta ggctgagtga
       61 tatctgtaac attatcacta gcctcctcaa gcattagcct acgagcaaat aaaaatggat
      121 tagctttacc ttgaatatta tctaatcgta atgcgaaacg aatatccgca agctcaatac
      181 ttaagtaatc aataatcacc gccaccattt ccacattatc tgcaaatgta cggctaaatt
      241 gtaattcact cagtccgatt gctcttgcat tttttgttaa ttcattgcgt ggcattgcac
      301 attcctttgg gtaaattaat cccacagatc ggggcgaagc tcagatttgg ctacttcgcc
      361 attcgtgact tcttcaattt tcttagcaag ataggctggc attggtgaat accctgtttc
      421 aacttgtcgt aaataagatt tggaaatacc aagctttttt gcaaagtcag ctttgaaacc
      481 acgagggcga tctgacaaat aactagttaa gttcattaac actcccgtta agtaaaaatt
      541 agattgagtt tagatttaac taaatacaaa gtcaagtaaa gtttagcttt tgcttgttta
      601 ggaaattcta aataaaataa tttaaaaatg aaaagtgagg ttaattatga ttaaaggtat
      661 gaccctgcca gagattcgca gaaaaaaact tacagaatgg ttcaacacaa gagatattcc
      721 agaaaaggaa aaaagctaca tatcacaatt aaaatcagga aaatcatctt ttggtgagcg
      781 tgcagcaaga agattagaga atgaatatgg aatgcctagc ttttatttgg actatgatga
      841 agaaacagaa agtgcgattg tgtctgctat gtcaattcaa gaaacacagt ctcatcaata
      901 ccccattcat ctaattgact tcaaagctaa ggcgggcgaa acaggttttg tcaatactaa
      961 ctatcctgaa attattcaat ctatctattt ctctttagac ggcttgcttg aaattgtagg
     1021 gcgaaaatca agcaatggca ttgagatgat caccattcca actgatagta tgagcccaac
     1081 aataaacaaa ggggatgttg tttttattga tactacaatc aattattacg ataacgaggg
     1141 tgtttatatt tttgttattg atgatgaagt ttatataaaa agactacaaa aaattccagg
     1201 tggtatttat agagccctat cggacaacaa gacttacgaa ccttttgata taaagagaga
     1261 aaccttagat actgctatta tccttgggaa atttattcga gtattgccaa taaatcctaa
     1321 agacttgtag gcattagcgt agcggttgag catcggagct atttgtagcc agcggtt
//
LOCUS       DANLZC010000241         1310 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.244, whole genome shotgun sequence.
ACCESSION   DANLZC010000241 DANLZC010000000
VERSION     DANLZC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.244"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(62..1264)
                     /gene="tet(H)"
                     /locus_tag="PXE44_002545"
     CDS             complement(62..1264)
                     /gene="tet(H)"
                     /locus_tag="PXE44_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDL5589996.1"
                     /translation="MNKSIIIILLITVLDAIGIGLIMPVLPTLLNEFVSENSLATHYG
                     VLLALYATMQVIFAPILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRI
                     IAGITGATGAVCASAMSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMP
                     FIFAAISHSILLILSLLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATY
                     FIIQLIGQIPATIWVLFTQYRFDWNTTSIGMSLAVLGVLNIFFQAIVAGKLAQKWGEK
                     TTIMISMSIDMMGCLLLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGK
                     LQGTLVSLTNITGIIGPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTT
                     PKAVISTP"
BASE COUNT          435 a          262 c          268 g          345 t
ORIGIN      
        1 aaagtcctat caacgttatt aagagcggac aggactttta tgatctttat aatttctagt
       61 attaaggggt tgaaataaca gctttaggtg tggtttttct ttggtgaaaa taagcggtaa
      121 taagcaacat agcataaagt attgccccca tcagccataa cagaccatcc caataagcga
      181 cgctataact ataaataaag gcaaataaaa ggggaccaat gatcccggta atattggtta
      241 ggctcaccag agtaccttgt aatttccctt gcgcattatc atcgacagat tttgataaat
      301 aaccttgtaa tgcgggttgc cccatacctc ctgccgctaa gcaaattaat gctggtaaga
      361 tgacccaaac gtggcctatc cacgctaata ataaacagcc catcatatca atagacatac
      421 tgatcataat ggtggttttt tcgccccatt tttgtgccaa tttcccagcg acaatcgcct
      481 gaaagaaaat atttaataca cccagaaccg ccaaagacat accgatagaa gttgtgttcc
      541 aatcaaaacg atattgtgta aacagcaccc agatggtggc aggaatttgc ccgataagct
      601 ggataataaa ataggttgct aaccaaaagt agaggctttt cttaaaaaaa acagtgactg
      661 tatttgaggc agtttggttt tcaggtgtcc tattggcaac aagcgcttct cttttttgtg
      721 tttctcggaa aaagagcaaa gagagtatta ataatatcga gtgtgaaata gcggcaaaaa
      781 taaatggcat atgagcactg atatcaccta ataatccccc tagcattggg ccgataataa
      841 ggccaacacc aaaagcacca cctaagaaac caaaatagcg agttcgattt ttagcgggag
      901 tcacatcact catcgctgat gcacatacgg cacctgttgc gcctgtgatc cccgcaatga
      961 tgcgcccaat atagagcatc caaagtgtgg ttgagaatgc cattaaaaga tagtcgagtg
     1021 ccgcgcctaa aagggaaaac agcaagatgg gttttctgcc gtatttatca gacagtcgtc
     1081 ctagaatagg agcaaaaata acctgcatgg tagcatagag cgctaatagc acaccgtaat
     1141 gggttgccag tgaattttca ctgacaaatt catttaatag agtagggagt actggcatga
     1201 taagcccgat accaatggca tctaatacgg tgatcagcag tataataata attgatttat
     1261 tcattgagat cctaaaaatc tatcagtgat agagtgggtg taatatatct
//
LOCUS       DANLZC010000242         1294 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.245, whole genome shotgun sequence.
ACCESSION   DANLZC010000242 DANLZC010000000
VERSION     DANLZC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.245"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..209)
                     /locus_tag="PXE44_002546"
     CDS             complement(<1..209)
                     /locus_tag="PXE44_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589997.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKAITDAPLT"
     gene            complement(332..>1294)
                     /locus_tag="PXE44_002547"
     CDS             complement(332..>1294)
                     /locus_tag="PXE44_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5589998.1"
                     /translation="LGRIGNSEVITGYSAHQGEFKNMKRVKVVFTAPTSGCIRFALRL
                     DNIQGKANPFLFARRPMLEEASDNVTDITQPSPWQNAGVTAIHGGSIVTNTVTAQQIA
                     ANTITANEIATGTIAARNMAANSINASHIVGSSITADKLNVNNLAAISANLGKVTAGT
                     ITGTTITGNTISGGSINGTTISGTTISGGTIKGARLEGATGKFTGELEVSQLIGGGVI
                     EQVTGKLKYSHRNRIYEGQEHGENSSSPIYRHEYIYKATVTINAAPTRRYIEIIGSES
                     FILEANQSITKTIEKSLNRNASGSSLDFIVLAYSISTAKYISIS"
BASE COUNT          345 a          266 c          250 g          433 t
ORIGIN      
        1 tttgttagcg gtgcatcagt aatcgccttg gcggactgtg catcaatgta tttgtactca
       61 cttgtgatga ttacaaagtt tgttacttct ccatcggtat catcgcctgt accgaccaca
      121 tatttagcat tgagcgagcc gtcatcttgc gttgagtagc ttgcgatggt ggaatacatt
      181 gggtttaaga ttttgttaaa agttgtcata atttttcctt ttatcgatag ttgaaataaa
      241 aacaatcgcc tgaattgacc tgcaagcggt tggttttgga tatttaaaaa agctgtggct
      301 atccgcactc agtactacat tgaggcgata actaactgat agagatatat ttagccgtcg
      361 aaatggaata ggctaaaaca ataaaatcta aagagctacc acttgcattt ctatttaatg
      421 atttctctat tgtttttgtg attgattgat ttgcttctaa aatgaatgac tcacttccga
      481 tgatttcaat atacctcctt gtcggcgctg catttatggt gacagttgct ttatagatat
      541 attcgtgtct ataaattgga gaggaggagt tttctccatg ttcttgcccc tcataaatgc
      601 gattacgatg tgaatatttt aatttgcctg taacctgctc tataacacct ccccctatta
      661 attgagatac ttcaagttcg ccggtaaatt tgccagtagc cccctccaat cttgcaccct
      721 ttattgtgcc gccagagata gttgtaccgc taattgttgt accattaata cttccgccac
      781 taattgtatt accagtaatt gtcgtacctg taattgtacc agcagttacc ttaccaagat
      841 ttgccgatat tgccgctaaa ttgtttacat ttagcttatc cgcagtgatc gaactcccca
      901 caatatgact tgcattaatc gagtttgctg ccatatttcg agctgcaatc gtgccggttg
      961 cgatttcgtt ggcggtaatg gtatttgctg caatttgttg agcagtaacg gtattagtaa
     1021 ctattgatcc accgtggata gcggttacac cggcattttg ccaagggcta ggctgagtga
     1081 tatctgtaac attatcacta gcctcctcaa gcattggcct acgagcaaat aaaaatggat
     1141 tagctttacc ttgaatatta tctaatcgta atgcgaaacg aatacagcct gaagtaggtg
     1201 cagtaaatac aaccttaacc cgtttcatat ttttaaattc gccttgatga gcactatagc
     1261 cagtaattac ctcgctgttg cctatccgac ctaa
//
LOCUS       DANLZC010000243         1281 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.246, whole genome shotgun sequence.
ACCESSION   DANLZC010000243 DANLZC010000000
VERSION     DANLZC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.246"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            233..508
                     /locus_tag="PXE44_002548"
     CDS             233..508
                     /locus_tag="PXE44_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5589999.1"
                     /translation="MAKATVARRIKNAINGNFGDHKSLAGTGGLYEMRIATGAGYRIY
                     YAQQGNVIYILLHGGDKSTQQADIEKAKALWAELKTTGESNGNNTNQ"
     gene            486..809
                     /locus_tag="PXE44_002549"
     CDS             486..809
                     /locus_tag="PXE44_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5590000.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNNVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
BASE COUNT          455 a          237 c          239 g          350 t
ORIGIN      
        1 atctacccaa attgtaattt tatgctacat aatttaaaag cgtgaacaat gttcaatgat
       61 atgaataatt gaaactgctc tcttttcaaa atttaaccac ttgattattt gtatcttaat
      121 ggatacacta agcaataaat agaataagag gctggcaatg acagagcaaa ccactaatca
      181 aatcatcatt accgataatt tttcaaaata gcttgagaat ttaaagaacc ctatcgcaaa
      241 agcaacggta gcaaggcgga taaaaaatgc gattaatggc aactttggcg atcataaatc
      301 tttagccggt acaggcggac tttatgaaat gcggatagca acaggagccg gatatagaat
      361 ttattacgca caacaaggaa atgtaattta tatcttgtta cacggcggcg ataaatcaac
      421 acagcaagcc gatatagaga aagcgaaagc attatgggct gaattaaaaa ctacagggga
      481 aagcaatgga aacaatacaa atcaatgaac aggcagcaga aaagcacggt ttacgccctt
      541 ttgacgtggc ggagttttta accgatgagg caactattca agcctattta gcggaagtgt
      601 taaaagaggg cgaccaagac gaatttttag aagccttgaa caatgtggca agggcaaggg
      661 gaatggcgga gcttgccaag caaacagggc tagggagaga gagcctatat aaaaccctag
      721 ccaaaggcag caaaccacgc tttgacacta ttcaaaaaat tctaggggca ttcaatttag
      781 tgcttattcc acaagtcaaa aatggctaaa acttatcact tgctacctgc caaaacctca
      841 cattcaaata gccgttgatt gatattaacg gcttttttat tgcttaattc tgtaccaatt
      901 acgcaccgat tgcggatcaa tacggggcaa gttttcgcaa aaccttaatg aaatatgacc
      961 gcttgtgatt ttgtacaaaa cgacacaaac caatgtataa aatcaaaact tgccacaaat
     1021 ggcggaatga tattaaatgc cccacaaact agcagtattt cttaatgact tttcatttta
     1081 agggtacatt taagggtatg tttaacaagt cttaaatgca cttctcaata ataacaagcc
     1141 tcatagcata caattcaaac tcggctgggg accatttaag cgttgcatat tgtctttcac
     1201 tgtctcaaat ttactataaa atcaagaact tagattattt tatagtctct tattgtcttc
     1261 tttagttgca ttgtatctta g
//
LOCUS       DANLZC010000244         1170 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.247, whole genome shotgun sequence.
ACCESSION   DANLZC010000244 DANLZC010000000
VERSION     DANLZC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.247"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..213
                     /locus_tag="PXE44_002550"
     CDS             <1..213
                     /locus_tag="PXE44_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDL5590001.1"
                     /translation="LKNLKYSPNAKPIADSNGLFILAQKKAKVWIYSYISPKTGKRST
                     KNRIGSYPQMSLVEQSPQVFEQIARL"
     gene            265..852
                     /locus_tag="PXE44_002551"
     CDS             265..852
                     /locus_tag="PXE44_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HDL5590002.1"
                     /translation="MFDELKIAQATAYLLHKAGGTMEHIKVMKLLYLADRLSWQRYGY
                     SISNDEYYSLPYGPVLSNTLNLMRGEEASLYQKTSIWGEWIADKEDHKISLKKSVNTE
                     DEYFFDCLSESDTEILEEVFATFGNYKPFALVEYTHNPKYIPEWEDPQGRSKPISLST
                     LLTKLGKTPSEIESILEQQAEKGTMDNLFKGLGNA"
     gene            845..1162
                     /locus_tag="PXE44_002552"
     CDS             845..1162
                     /locus_tag="PXE44_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590003.1"
                     /translation="MLEIKRQVTILRPTNYGKEENHYKHLYILLTDPCFDGETGKGDM
                     ILSVNCSSIKDGKALDETCILKAGTHEFITRDSFIFYRHIKIDSLDEIKANIEAGYFI
                     QKP"
BASE COUNT          425 a          221 c          237 g          287 t
ORIGIN      
        1 ctaaaaaatc ttaaatattc cccaaatgcg aagcctatcg cagacagtaa cgggctattt
       61 attcttgcac agaaaaaagc gaaagtatgg atttatagct atatcagccc caaaacaggc
      121 aagcgaagca caaaaaatag aatcggtagc tatccgcaaa tgagcttagt ggaacaatcc
      181 ccacaggtat ttgaacaaat cgcacgtctt taaaatatta aattaagaga aagcactatt
      241 tgcgatgtag caggagataa aacaatgttt gatgaattaa aaatagccca agcgaccgcc
      301 tatttgctac ataaggcagg cggaacaatg gagcatatta aggtaatgaa attgctttat
      361 cttgcggatc gcttatcgtg gcaaagatac ggctattcta tttctaatga tgaatattat
      421 tcattgcctt atggaccggt attaagcaac acactcaacc taatgcgagg agaagaagcg
      481 agcttatacc aaaaaaccag tatttggggc gagtggatag cagataagga agatcacaaa
      541 atcagcctta aaaaatccgt aaataccgaa gatgaatact tctttgattg cctttcagag
      601 agcgacaccg aaattttaga agaggtattt gcgacctttg gaaattataa gccattcgcc
      661 ctagtggaat atacccataa ccctaaatac attcccgaat gggaagaccc acaaggcaga
      721 agcaagccga tttctttaag cacgcttctt actaagctag ggaaaacgcc aagcgagatc
      781 gaaagcattt tagagcaaca ggcggaaaaa gggacaatgg ataacctttt caagggctta
      841 ggcaatgctt gaaatcaaaa gacaagtaac gattcttaga ccgaccaact acggcaagga
      901 agagaaccac tacaagcatt tatatattct gcttaccgat ccgtgctttg atggtgaaac
      961 cggtaaagga gatatgattt taagtgtaaa ctgctcaagc attaaagacg ggaaagcact
     1021 tgatgaaact tgtattttaa aggcaggaac acacgaattt atcacacggg acagcttcat
     1081 tttctaccgc catataaaaa ttgattcgtt agatgagatt aaggcaaata tagaagccgg
     1141 ttattttatt caaaagccct aattaatgat
//
LOCUS       DANLZC010000245         1106 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.248, whole genome shotgun sequence.
ACCESSION   DANLZC010000245 DANLZC010000000
VERSION     DANLZC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.248"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            259..>1106
                     /gene="msrA"
                     /locus_tag="PXE44_002553"
     CDS             259..>1106
                     /gene="msrA"
                     /locus_tag="PXE44_002553"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL5590004.1"
                     /translation="MKYNQLLLCLVSAIFTTSSIANITTPNEMRSENLQDIYLAGGCF
                     WGIEAYMEKIYGVKEATSGYANGKTEQTAYRIIGQTDHAETVHVTYDANKISLEKLLQ
                     YYFKVIDPTSKNKQGNDIGRQYRTGIYYQNEQDKQIILKEIQAQQAKYKQKIQVQVEP
                     LKHYILAEEYHQDYLKKNPNGYCHIDLNQANSIIIDPKDYPKPSDATLKMKLTPLQYS
                     VTQRKNTERSFSNEYWDNTAPGIYVDITTGEPLFSSLDKYDSGCGWPSFTKPIINEVV
                     NYTEDN"
BASE COUNT          424 a          190 c          186 g          306 t
ORIGIN      
        1 aattgtaact gagcaaattt caacatttgc tgacgaatat cggcaagctg ttccgatgaa
       61 atatctaaag aaatcttgtt atgaatcata gtgttatatt gagaaaattt tttgtgagaa
      121 tattaaacaa tattcgacta atcctataga gagtaaaacc aattttgatc ttagcaaaaa
      181 agtaagaaat tatgaatgtt taaggttaaa ccctcatcct taaaaaataa aattacataa
      241 gttataaagg agaactggat gaaatacaat caactgttac tatgtttggt gtctgctatc
      301 tttaccacct cctcaatagc aaacatcaca acccctaacg agatgcgatc tgaaaactta
      361 caggatattt acttggcagg aggatgtttc tggggcattg aagcctatat ggaaaaaata
      421 tatggcgtaa aagaggctac atcgggctat gccaatggca aaactgagca aactgcttac
      481 cgaattatcg ggcaaaccga tcatgcagaa acagttcacg tgacctatga tgctaataaa
      541 atttctttag aaaagctgtt gcaatattac tttaaagtaa ttgatccaac cagtaaaaat
      601 aaacaaggta atgatattgg ccgccaatat cggacaggta tttattatca gaatgagcaa
      661 gataaacaaa ttattttaaa agaaatacaa gctcaacaag caaaatacaa acaaaaaata
      721 caagtgcaag tagaaccatt aaaacattat attttggcag aagagtatca tcaggattac
      781 ctaaagaaaa atccaaatgg ctactgccat attgatttaa accaagccaa cagcattatt
      841 atcgatccga aagattatcc taaacctagt gatgcaacat taaaaatgaa attaacgcct
      901 ttgcaatata gcgttaccca acgtaaaaat actgagcgtt cattcagcaa tgaatattgg
      961 gataataccg caccgggcat ttatgttgat ataactacag gagagcctct tttttcatct
     1021 ctcgataaat atgactccgg ctgtggctgg ccaagtttta ccaaaccaat tatcaatgag
     1081 gttgttaact ataccgaaga taatag
//
LOCUS       DANLZC010000246         1068 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.250, whole genome shotgun sequence.
ACCESSION   DANLZC010000246 DANLZC010000000
VERSION     DANLZC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.250"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..850
                     /locus_tag="PXE44_002554"
     CDS             <1..850
                     /locus_tag="PXE44_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5590005.1"
                     /translation="RTYMNDNVIPFMEKVEEFTETLKTLSRDDLSAEERTTKTIDSVK
                     LRIYLNEQARNRKNYINGFVPTDWIPEITDQMTKSKNLWLKYEEEAKTTLKPYQEETI
                     SRVIQDEINKKMAEIGTTTLEIGGKEHELEDLENQINALALTDEEKLAEDVENDLISK
                     LDEAKAALAKNQQTLKEKRDELLGLNNKLDSSPLVKKGKNALAEGTNAFASGENAIAF
                     GTDSQATGNNAIALGANSKANAESAIAIGKGAQALKEKALALGENAIANRASAIAIGD
                     NHSSKL"
     gene            complement(899..>1068)
                     /locus_tag="PXE44_002555"
     CDS             complement(899..>1068)
                     /locus_tag="PXE44_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL5590006.1"
                     /translation="YTDLTTLQADLDEWLMYYNHHRTHQGKMCCGRTPMATLLDGKRI
                     WVEKEFKLKLI"
BASE COUNT          402 a          191 c          194 g          281 t
ORIGIN      
        1 tcgcacttat atgaatgata atgtaattcc ttttatggag aaggtagagg aatttactga
       61 aacattaaaa actttatcta gagatgactt aagtgcagag gaacgtacaa ccaaaacaat
      121 tgactccgtt aaattaagaa tatatctcaa tgaacaagca cgaaatcgta aaaattatat
      181 taatggattt gtacctaccg attggattcc tgaaattaca gatcaaatga caaaatccaa
      241 aaatctatgg ttaaaatatg aagaggaagc caaaacaaca ttaaaaccat accaagaaga
      301 aacaatatct agggttatac aagatgaaat taataaaaaa atggctgaaa taggtactac
      361 aacactagag attggtggta aagaacacga gctcgaggat cttgaaaacc aaattaatgc
      421 tttagcttta actgatgaag aaaaacttgc ggaagatgtg gaaaatgatt taatctcaaa
      481 gcttgatgag gctaaagcag ccttagcaaa aaaccagcaa acgctcaaag aaaagcgtga
      541 tgaattacta gggctaaata acaaattaga tagctcacca cttgttaaaa aaggtaaaaa
      601 tgcacttgca gaaggcacga atgcctttgc ttctggtgaa aatgccatcg cattcggcac
      661 ggattcacaa gctacaggta ataacgctat cgcactaggt gcaaattcaa aagcaaacgc
      721 agaaagtgcg attgcaattg gtaaaggtgc acaagcatta aaagaaaaag cccttgcttt
      781 aggcgaaaat gccattgcca acagagcatc tgctattgcg attggtgata accattcatc
      841 taaattgtag aagatcaaac ctaatctgac agtcccccgt tttaaattac cgtgtctgtc
      901 agattaattt gagcttaaat tctttttcca cccaaatccg ttttccatca agtaaggttg
      961 ccatcggtgt tctgccacag cacatttttc cttgatgtgt tcgatggtga ttataataca
     1021 ttaaccactc atctaaatcc gcttgtaatg tcgttaaatc cgtataaa
//
LOCUS       DANLZC010000247         1042 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.251, whole genome shotgun sequence.
ACCESSION   DANLZC010000247 DANLZC010000000
VERSION     DANLZC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.251"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(25..>1042)
                     /locus_tag="PXE44_002556"
     CDS             complement(25..>1042)
                     /locus_tag="PXE44_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590007.1"
                     /translation="FLDKGFIPNDNYRDIIALLPVALYVFHLRSLKHIKNLESNSYLD
                     EEGRDTVNKYKAFFSIFEAEIRDATCICDNKIVTINGEVCCDIKEVARKIVIEFLNEV
                     DIGNKVKFYYPDERHELFVELLIVLATLRIYRNRNIFFFEKGFFNFFPEFITILGTGA
                     FNGFLDFPSLVGKVFPEIIDTINEGLDLDIAIENFEVLSQEEMTECMGLYEQALIKKA
                     EEGEIGLLNVDEINSFYEDNSELILLAKQPMVEEIIPKPDSKASSAILKFFDSNSNIL
                     IAKLKESGLQLSAVALQNDQNIARVANVVYNLLPGMVRIFVSYDTVENFLLNNRQWLI
                     NKLV"
BASE COUNT          349 a          205 c          126 g          362 t
ORIGIN      
        1 tatccatagt tcttcccctc caaattaaac taatttatta atcaaccatt ggcggttatt
       61 taataagaaa ttttctacgg tatcgtaact tacgaaaatt cttaccatgc ccggcaataa
      121 gttataaacg acatttgcta ctcgtgcgat attttggtcg ttttgtaatg ccacagcact
      181 taattgcaaa ccactttctt ttaatttagc aattaagata tttgagtttg aatcaaagaa
      241 ttttagaatt gcagaggagg ctttgctatc tggtttaggg attatctctt caaccatagg
      301 ttgtttagca agtaagatta gctcactatt atcttcataa aagctattta tttcatcaac
      361 atttaataaa ccaatttcgc cttcttcagc tttcttaatg agagcctgct catataaacc
      421 catacattca gtcatttctt cctgactcaa cacttcaaag ttctcaatcg caatatctaa
      481 atctaaaccc tcatttatcg tatcaataat ttctggaaaa acctttccta ccaaactagg
      541 gaagtctaaa aatccattaa acgcaccagt cccgagaata gtgataaatt cgggaaaaaa
      601 gttaaaaaac cccttttcaa agaagaatat atttctatta cgatatattc ttaatgttgc
      661 tagaacaatg agcagctcta caaaaagctc atgtctttca tctgggtaat aaaacttaac
      721 tttattacca atatccactt catttagaaa ttctataaca attttccttg ctacctcctt
      781 aatatcacag cacacctcac cattaattgt tacaatttta ttgtcacaaa tacatgttgc
      841 atctcgaatc tctgcttcaa aaatagagaa aaatgccttg tatttattta ccgtatctct
      901 tccttcttca tctaaataac tgttagattc tagatttttg atatgtttca aagatcttaa
      961 gtgaaagaca tataacgcta ctgggagtag agcaataata tctctataat tatcattagg
     1021 tataaatcct ttatctaaaa ac
//
LOCUS       DANLZC010000248         1034 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.252, whole genome shotgun sequence.
ACCESSION   DANLZC010000248 DANLZC010000000
VERSION     DANLZC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.252"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            146..394
                     /locus_tag="PXE44_002557"
     CDS             146..394
                     /locus_tag="PXE44_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590008.1"
                     /translation="MISGEKMTWESLGASAIGGAIGGGIGATMKAGAVTASTLSALGG
                     GMGEGLYKSTSQGIKNSIGIKASCNQSGSDYGDGCDYQ"
     gene            406..609
                     /locus_tag="PXE44_002558"
     CDS             406..609
                     /locus_tag="PXE44_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590009.1"
                     /translation="MKIQFPISYQEFRENYFEKKPLLMKGAISQKDLLSWKSINEILP
                     RCDLISEDAIKVMHKGKRAHKKD"
     gene            648..1034
                     /locus_tag="PXE44_002559"
                     /pseudo
     CDS             648..1034
                     /locus_tag="PXE44_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          334 a          142 c          252 g          306 t
ORIGIN      
        1 atagtatttg ttgtaattat aaacatcata gaggaatatc tcttatgaaa gagttaaaaa
       61 ttgaggattg ccaatttgtt tcggcaggct tgcacttgtt attgtaggag ctgttgctgg
      121 agctgcatat tatgttggga atggtatgat ttctggggaa aaaatgactt gggagagttt
      181 gggagcatca gctataggtg gggctattgg tggcggtatt ggagcaacaa tgaaagctgg
      241 tgcggtgaca gcgtcaacct tatctgcttt aggtggtggt atgggggagg gcttgtataa
      301 aagtacttct caaggtatca aaaatagtat tggtataaag gcgagttgca atcaatcagg
      361 ttcggattat ggtgatggat gtgattatca ataagagagt atattatgaa aattcaattt
      421 ccaatttctt atcaagaatt tcgtgaaaac tattttgaga aaaaaccact tttaatgaaa
      481 ggagcaatat ctcagaaaga tttactttct tggaagtcaa taaatgaaat attacctcgc
      541 tgtgatttaa tttctgagga tgcaattaag gtaatgcata aagggaaaag ggctcataaa
      601 aaagattagg ggctgtagta gattagcatc actgctatac tacaaaaatg aagataaccc
      661 attgtaaatt aaagaaatct ttgcagaaaa agcttcttga attttttgtt gctgaagtga
      721 ccgctagaac cgctgctgat ttattaggta ttcaacccaa tacggcagcc ctgttttacc
      781 acaaaattag gctggtcatt gaacaccatt tagcccttga agctcatgaa atttttgagg
      841 gaaaaattga ggtagatgaa agctattttg gtggtcatcg caaaggaaaa cgagggcgag
      901 gtgcggctgg gaaagtcgct gtattcggtt tgttgaagcg tcaaggaaag gtgtttacaa
      961 aataccaaat cagaaacgct aatgccggtt attacaagga aaatcaagcc tgacagctgg
     1021 gtttatacgg atac
//
LOCUS       DANLZC010000249         1024 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.253, whole genome shotgun sequence.
ACCESSION   DANLZC010000249 DANLZC010000000
VERSION     DANLZC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.253"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            45..959
                     /locus_tag="PXE44_002560"
     CDS             45..959
                     /locus_tag="PXE44_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590010.1"
                     /translation="MKNVHTKYDHKAVKHGFAIAIQNSVNYLYEIAERQPPCNFELCW
                     KNCAAHYLAIKSAESNNERDLNLFLDYINEPKDSTFTVAPLYKDNTLETRMILEIIKD
                     DNQIGFGFCEASDLSLIEDETKKIKAAMSLIDDLDPDTSNEIKCFIDTIYLTKEGLNG
                     ERFMRSGTNFYMWGMMFLYINKEHSILYYADHIIHECAHTALNIINATDELVTNSADE
                     RFKAPLRKDTRPMIGLFHALFVLHRICSSFQKMKEKIDDVYYDEFNHRLNDNLLKLHE
                     TYQTVRENAKTTEIGEKILNEIAKTWKL"
BASE COUNT          377 a          133 c          175 g          339 t
ORIGIN      
        1 gaaacctact taacagtagg tttctttaac aaattgaggt ttttatgaaa aatgtacata
       61 cgaaatatga tcataaagca gtgaagcatg ggtttgctat agcaatccaa aattctgtta
      121 attatttata tgaaattgca gaacggcagc ctccttgtaa ttttgaacta tgctggaaga
      181 attgtgcagc acattattta gcaataaaat ctgcagaaag taataatgaa agggatttaa
      241 atttattcct tgattatatt aatgagccga aagattctac atttacagta gctcctttat
      301 acaaggataa tacactagaa acaagaatga tcttagaaat tattaaagat gataaccaaa
      361 ttgggtttgg attttgtgag gctagtgatt tatctttaat tgaggatgaa actaaaaaaa
      421 tcaaggcagc aatgagctta attgatgatt tagaccctga tacttctaac gagataaaat
      481 gtttcattga tacaatttat ttaacaaaag aaggccttaa tggcgagcga tttatgcgtt
      541 ctggcacaaa tttttatatg tggggaatga tgtttttgta tataaataag gaacactcta
      601 ttctttatta tgcagatcat ataattcatg aatgtgctca tacagctctt aacattataa
      661 atgcaactga tgaattagta acaaattcag ctgatgaacg ttttaaagca cctttaagaa
      721 aggatactcg tcctatgatt gggctatttc atgcgttgtt tgtactccat agaatatgtt
      781 cctcctttca aaaaatgaaa gaaaagatag atgatgttta ttatgatgaa tttaaccata
      841 gattaaatga taacctgttg aagttacatg aaacatacca gactgttaga gaaaatgcaa
      901 aaacgacaga gattggtgag aaaattttaa atgaaattgc caaaacttgg aaactttaat
      961 caatctgaaa ttatgatgag aatgttagtt ttctttaatt caatattctc atctacatta
     1021 attg
//
LOCUS       DANLZC010000250         1009 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.254, whole genome shotgun sequence.
ACCESSION   DANLZC010000250 DANLZC010000000
VERSION     DANLZC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.254"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..>1009
                     /locus_tag="PXE44_002561"
     CDS             <1..>1009
                     /locus_tag="PXE44_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590011.1"
                     /translation="FILFDSILERHLGESLKRALESLGHQAVFTDLILHGHSMIYKQA
                     DIDFMWKKVKDICDEKADMFISFRPMNLTTEMLEYIGKRMKTAIWLSDDPVLYKTCYS
                     QVVNHYDVLLHCGYKEVMDFYEANNHPKGFNFPFWTDHVAFPSVYNPLHSDYEIAFLG
                     NMNGQVRRKRYMELASLPFTKKVFGLIDSDPLAMHGGFINEAYLHTKRVTEVLSKAKV
                     GVSIPQFFTEYNGLDYDYPELAGLGYFQFPSRVIQYAASGLPIAAVGDERMKEVYPEI
                     FVGNSITELEPYIKQICEDYDFALSESAKILTRFRKNYSALSRAMMLIDLVENLDKLQ
                     SQ"
BASE COUNT          328 a          144 c          202 g          335 t
ORIGIN      
        1 tttattttat ttgattctat tttagaacgt catttgggcg aaagtttaaa aagagctttg
       61 gagtcgttag ggcatcaagc agtatttaca gatttaattt tgcacggcca ttctatgatt
      121 tataaacaag cggacataga tttcatgtgg aaaaaagtaa aagatatttg tgatgaaaaa
      181 gcagatatgt ttatctcatt tcggccgatg aatctaacga cagaaatgct tgagtatata
      241 ggcaagcgaa tgaagaccgc tatttggctt tcagatgacc ctgttttata taaaacttgc
      301 tattctcaag tagtaaatca ttatgatgta ttattacatt gcggatataa agaagttatg
      361 gatttttatg aagctaataa ccatccaaaa ggctttaatt ttccgttttg gacagatcat
      421 gtagcatttc caagtgtata taacccgctt cattctgatt atgagattgc atttttaggt
      481 aatatgaatg ggcaagttag acgcaaaaga tacatggaat tggcttcatt accatttacc
      541 aaaaaagtat tcggtttaat agattcagat ccattagcaa tgcatggtgg atttatcaat
      601 gaagcatatt tacatacaaa acgtgtaaca gaggttttat ctaaggcaaa agttggggtg
      661 agtatcccac aattctttac cgaatataat ggtttagatt atgattatcc tgaattggcg
      721 ggtttaggat atttccaatt tcctagtcga gttatccaat atgctgcaag tgggttacct
      781 attgcagcgg ttggtgatga gagaatgaag gaagtttatc ctgaaatctt tgttggcaat
      841 agtattacag agctggagcc gtacattaaa caaatttgtg aagattatga ttttgcacta
      901 tcagaatcgg ctaaaattct aactagattt agaaagaact attctgcctt atcccgtgcc
      961 atgatgttga ttgatttggt agaaaattta gacaaactac agagccaga
//
LOCUS       DANLZC010000251         1006 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.255, whole genome shotgun sequence.
ACCESSION   DANLZC010000251 DANLZC010000000
VERSION     DANLZC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.255"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..>1006
                     /locus_tag="PXE44_002562"
     CDS             <1..>1006
                     /locus_tag="PXE44_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDL5590012.1"
                     /translation="FTAEWTSERAYLLVGVNGTYETTDWLAFHSVKLEKGNVATDWTP
                     APEDVDSAVSAVSAKIDTIQETLANADSALSSRIDAVTASVGSNLAKITQVSNAVAGV
                     DGKLSATHTIKTETIAGGRKAIAGIALGAAADNRNAESSVIVMADKFEVVKNAQDGQP
                     VRLFGVAQNKVAINGDLIATGTIKGSHIDADSVRAGILTAGAIRTEHLAAGQISADKL
                     AIGLGGNLLVNPIFATPDLSIAPFGWNYWRGEVGDRLNIDTRNIFNRNEQDNYGLKNG
                     GLPNELVFSMRYSTTKETSDTGRIGWLSQDINVVPNKKYIASVWLACHRGQAKLVIEN
                     "
BASE COUNT          294 a          192 c          255 g          265 t
ORIGIN      
        1 cctttactgc cgaatggaca agtgagcgag cctatctgct tgttggtgta aatggtactt
       61 atgagaccac agattggtta gcgtttcaca gcgtcaaact tgaaaaaggc aacgtagcca
      121 cagactggac gccagcacct gaagatgtgg acagtgcggt tagtgcagtt tcggctaaaa
      181 ttgatactat tcaggagact ttagcgaatg ccgactctgc attaagctct cgtattgatg
      241 ccgttactgc ttctgttggc tcaaacttgg caaaaattac tcaagtgagt aatgctgtag
      301 ccggtgtgga cggcaaatta tctgctaccc ataccatcaa aaccgaaacc attgccggtg
      361 gacgtaaagc gattgccggt atcgcacttg gtgccgcggc agacaatcgc aatgcagaat
      421 cctctgtcat tgtaatggca gacaaatttg aggtagtaaa aaatgctcaa gacggccaac
      481 cggtacgatt gtttggtgtg gctcaaaata aagttgcgat taatggggat ttaattgcaa
      541 caggtacaat taaaggtagc cacattgatg cagactcagt aagagccgga atattgacgg
      601 cgggagctat ccgtacggaa cacctggcag cagggcaaat atcggcggat aagttagcga
      661 ttgggctagg tgggaatttg ctggttaatc ctatttttgc aacacccgat ttgtcaatag
      721 ctccgtttgg ctggaactac tggcgaggag aagttggtga tcggctaaat attgacaccc
      781 gtaatatttt caataggaat gaacaagata attacggact aaaaaatgga ggactaccaa
      841 atgagttagt attttcgatg cggtatagca ctacaaagga aacctctgat actgggcgaa
      901 ttgggtggtt atctcaggat attaatgttg tgcctaataa aaaatatatt gcctccgtat
      961 ggttagcttg tcatcgtggg caagctaaat tagtcattga gaatat
//
LOCUS       DANLZC010000252         1000 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.256, whole genome shotgun sequence.
ACCESSION   DANLZC010000252 DANLZC010000000
VERSION     DANLZC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.256"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(474..731)
                     /locus_tag="PXE44_002563"
     CDS             complement(474..731)
                     /locus_tag="PXE44_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590013.1"
                     /translation="MNNKQILSIAQKFRQNHKDSHPFMLERGYVRIYQGKAYGWCAEQ
                     EDANTECPTALIVSLSNEIFEATGGDDISGAEKWEKYNSNF"
BASE COUNT          294 a          180 c          159 g          367 t
ORIGIN      
        1 tttcccgtgg ggagatactc tcccttcgga gcattgtgtc tcccttttta ttcacttaac
       61 tagaggacac aatgttcaat ctaaacttat ttaaacgccc tattgagctt tcaacgcttg
      121 aatcttggtc taaattctgt gatgatgtcg gtaaagttgc atttattgcg atacctccga
      181 tgatttatag taattatgat ctatgtatca aaataggaaa cataagtgct ttattggcaa
      241 ttacctatgg attcttatta tcaggcagga ctatccgtca atttattgag aaggagaaat
      301 caagatggga ttaacaatcg gcttatggat cattgctttt ataggctttg cactggtttt
      361 ttgggcaaat agcttactca aagagcaaga tgaacgcctt aaacagctaa aaaaatagct
      421 caactttaca ctcaatacgg cacatttggt gccgtatttc attcttatcc taattaaaaa
      481 ttagaattat atttttccca tttttcagcc ccacttatat catcacctcc agttgcctca
      541 aaaatttcat tagataagct gacaattaag gcggtagggc attccgtgtt tgcatcttct
      601 tgctctgcac accagccata ggcttttccc tgataaatac gaacataacc acgctctagc
      661 ataaaagggt gagaatcctt gtgattttga cgaaattttt gagcaataga caaaatttgt
      721 ttattgttca taattatttt tcttattgct tatatgctga aaattgatga ttgaatttat
      781 tatatctaat tataatattc aatcctgttg ctatactgtc taaatcgact atgtgttcat
      841 ccttttggtt cataaatgta catttctcta taataactaa aatgtttttg gtctctgaat
      901 tgtttattat ttcttttgct ggcattagct ccataaagtg agataacttc attcttattg
      961 ttctgaagta ccaatactta gtattgcaat atgctatcaa
//
LOCUS       DANLZC010000253          964 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.257, whole genome shotgun sequence.
ACCESSION   DANLZC010000253 DANLZC010000000
VERSION     DANLZC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.257"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            223..964
                     /locus_tag="PXE44_002564"
                     /pseudo
     CDS             223..964
                     /locus_tag="PXE44_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
BASE COUNT          343 a          167 c          151 g          303 t
ORIGIN      
        1 gactcctcta atagtccact cttgttccat tttggaacat ttttgtatat tatttcgcct
       61 tttctttctc attgtgttct atgtaggcaa cagcattttt tgcaaagtga tcgacgagaa
      121 cattcattac ttcagtccat tttacaggtc ttcctatctt cattccaact tccatcgcta
      181 atctttctat cttgagtttt ttctctaatt taatattgaa cagagcttta tgaaatggtt
      241 gatatagaac aaacgtcagt tgattggatt gatgtaactt attcagctca tattgattcg
      301 gaatctatcc aaaaacaact tatctctttt ttccaaaaca tccatttagg acaaacacgt
      361 cgtacaagac ataacaaaga atatgacaca acggcagaat ggaattcagg ttcagaacac
      421 cgtattttaa aagtttatct aaaaggtaat gaagttcaaa aaagactaca agaagcccag
      481 cacgacttca aaaaatcacc aagtaaaaag tatctgcaaa actcaattca agtattaagt
      541 aatccaaatc tcattgaatt ttcaaaaaaa tgtgttcgtt ttgaagcaag attaaaacaa
      601 cgttatttag ataaacatca aatcccacgc aatttatttg acttaattaa atatcagaga
      661 gattacgaag agcagggtag aagcctgatt aaagacctat ggcaagacgc attcaaagac
      721 attattaagg ctgtcgggga aagcaatatg aatatttaca acagagatca aattcagcgt
      781 ttattacgga aaaattattt ttcaatcact ccaaaaggaa atatgagcta tgcaaaggct
      841 gatcgtttat tcagctttta taagaattta ctaagtgatg gctatagaga aacgctagat
      901 tctatgccaa gaaacacatt ttggagatat gaaaaggatc ttatggccgt tggtttaact
      961 aaaa
//
LOCUS       DANLZC010000254          930 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.258, whole genome shotgun sequence.
ACCESSION   DANLZC010000254 DANLZC010000000
VERSION     DANLZC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.258"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            1..930
                     /locus_tag="PXE44_002565"
                     /pseudo
     CDS             1..930
                     /locus_tag="PXE44_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484596.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          322 a          135 c          161 g          312 t
ORIGIN      
        1 atggataaag gaagttcata aatattcaaa aggtggattt aagagatgtg ttaatatccc
       61 tttctttaga tttagtaagt ttttccttaa taatgcacaa cgaacagcct tttcatatgt
      121 aactcattac attaagaaac attcctatgc ctatagtaag gcagacaata ttcaagaatt
      181 attagtaaag tttttctcta gaaagcaacc tgttatgcaa agtgcattta atcatcaaaa
      241 aaggattgtt aagtggttat gggctgaatt ttgggataag aatattatct cgatgacact
      301 tagaattgat ggttatagaa atgctaactt atggcattat ttacgttatc aatcacggat
      361 tgaggtattg tataaaatag caaatgaaca cccgaatcta ctaccattat tagcctatgt
      421 taaacctgag tactggaaat gctcggactt atttagttat caaaattggg taagaagccg
      481 tgataaacag ccttttattt tgtatgctaa atgccaagat aagatgttat gtgaatcaaa
      541 ggggcaatgg agatggttaa aaaaacagtc gagaatagtt gtagatcaat gttttctaca
      601 tgttaatcaa tggcagtaat taacagaaat tcaaatgcct ttcaagatcc cagtatgtgt
      661 catatgtttc atacttacta attttagcat gatcagctgt gagttagatc gatctagaca
      721 acgtcgatta ctctctattt ttattcaata tatctatcat tattggaaag ataatgggtt
      781 tagacaatta aagatacatc tttctcatct gagagtgctt tttcaagata ttgaagatta
      841 catgatacat gaggggtatt tacatggtat acctcagact tggcaacatt tggtttacca
      901 cctcaacctt ataaattcaa atacatcaaa
//
LOCUS       DANLZC010000255          927 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.259, whole genome shotgun sequence.
ACCESSION   DANLZC010000255 DANLZC010000000
VERSION     DANLZC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.259"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            46..672
                     /locus_tag="PXE44_002566"
     CDS             46..672
                     /locus_tag="PXE44_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL5590014.1"
                     /translation="MTKIKFYGNLKKFGSEFSLEVKDTAEAIRALCTQISGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFNFVLGAALIGAA
                     FFTGGASIAAWGAGAKMMGMLGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSNL
                     NNLVTQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
     gene            721..>927
                     /locus_tag="PXE44_002567"
     CDS             721..>927
                     /locus_tag="PXE44_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5590015.1"
                     /translation="MNLTKAESNLNAKITEEKKARVDADKVNADKLTDITSRVANAES
                     TITNFQSTKANKSEVASLAQSSLQA"
BASE COUNT          307 a          173 c          210 g          237 t
ORIGIN      
        1 gaacataaat cccctttatt ttggctataa ttgtaatggt gagtcatgac taaaattaaa
       61 ttttacggca atttaaaaaa atttggtagt gaatttagct tagaagttaa agatactgcc
      121 gaagctatcc gagccttatg tacacagatt agcggattgc gtgaggcatt acgagacggt
      181 gtttataaag tccgtatcgg caaacagtat ttagacccgt cagcccttga aaaagggctt
      241 ttttattgtc tgaagaaagg gcaaaccatt cattttaccc ctgttatcaa aggagcgaaa
      301 agcggtggcg tgtttaattt tgttttaggt gcagccttga tcggagccgc attttttact
      361 ggcggtgctt ctattgcggc ttggggagct ggagcaaaaa tgatgggtat gttaggggct
      421 tcgatgttac taggaggagt ttcccaaatg ctcaccaaaa tgccaaaagc ccccacaatg
      481 ggtaatgaaa cagaaaaaga aagctcaacg gctttttcaa atttaaataa cttggtgact
      541 caaggcaaac ctgtgccgtt ggcttatggc ttaattcgga ccggctcgct tgtgatatcg
      601 cagggagttg agacgatcac cattaaagaa aaccaaccgg caagcaacaa gaaaacaggc
      661 tttagaaaat aagggataat taccgattca gcacaagcac agcaacttaa tcagttatct
      721 gtgaacttga ccaaagcaga gagcaattta aatgccaaaa tcacggaaga aaaaaaagct
      781 cgtgttgatg cagataaagt gaatgcagat aaactcacag acataacaag ccgtgttgca
      841 aatgcggaat caactattac caatttccaa tcaaccaaag cgaataaaag tgaggttgcg
      901 agccttgcac aaagttcgtt gcaggca
//
LOCUS       DANLZC010000256          802 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.260, whole genome shotgun sequence.
ACCESSION   DANLZC010000256 DANLZC010000000
VERSION     DANLZC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.260"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            7..753
                     /locus_tag="PXE44_002568"
     CDS             7..753
                     /locus_tag="PXE44_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL5590016.1"
                     /translation="MKYNELDVLEVNHNKFKAKIALQGAQLLSWQPYNAEQDVLWLSE
                     IEPFRIGSAIRGGIPICYPWFGSIKEPAHGTARIQEWRLVEHTCSDENVGLVFALENK
                     AKMEMMLGETCELYFTHLDPEPAQLALHSYFNIGDIEKVEVQGLPTRCFDKLTDQEVE
                     VPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETVLWNPWHNTVSAMTET
                     AYQNMVCVETARINTLLQQNETLGVRVLVR"
BASE COUNT          261 a          130 c          181 g          230 t
ORIGIN      
        1 aaattaatca aatataatga attagacgtg ctagaagtta atcacaataa attcaaagct
       61 aaaattgcat tacaaggagc acaattgcta agttggcagc cgtataatgc agagcaagat
      121 gtgctgtggt taagtgagat agaaccgttt agaattggct ctgcgattcg tggcggtata
      181 ccaatttgtt atccgtggtt cggttcaata aaagagcctg cccacggcac agctcgcatt
      241 caagaatggc gtttagttga gcatacttgt tctgatgaaa atgtcggttt ggtatttgcc
      301 ttagagaata aagcgaaaat ggaaatgatg ttaggcgaaa cttgtgagtt atattttacc
      361 catttagatc cagaaccagc acaactggca ttgcactctt attttaatat tggtgatatt
      421 gaaaaagtgg aagtgcaagg cttgccaact cgctgttttg ataagctaac cgatcaagaa
      481 gttgaagtgc catcacctcg caagataagt gaaaatgtgg attgtattta tccggcacaa
      541 gcggtgaatt ttattcaaga ttttgcaaat cagcgcacca ttaaagttga gcatattaat
      601 gcgaccgaaa ccgtactttg gaatccttgg cataataccg ttagtgcgat gacagaaacg
      661 gcgtatcaaa atatggtatg tgtggaaacc gctcggataa atactctact tcaacaaaat
      721 gaaacgctag gagtgagggt tttagtgcga tagtgtttaa tgagagcaag cagctgaatt
      781 agtaatattg tttacaaatt ta
//
LOCUS       DANLZC010000257          797 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.261, whole genome shotgun sequence.
ACCESSION   DANLZC010000257 DANLZC010000000
VERSION     DANLZC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.261"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..>797)
                     /locus_tag="PXE44_002569"
     CDS             complement(<1..>797)
                     /locus_tag="PXE44_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5590017.1"
                     /translation="QSSYGKIQINRNARPTGQYNLNNEELGAIKVPLIDLETQKQVDK
                     IIKQSNDFVQKSTQSYQEAETLLLENLGLRAFQADSNPVNVKSLKESFLQTGRLDAEY
                     YQTKYEQYWNLIQSQDYVFIRDEYLHITQKPDWTKPMYQYIEIGDVNIGDGSYQTNWI
                     ETQELPANAKTQAQTGDILISTVRPYRGAVTIIGENDQDLVVSGAFTVLRRKENSVFN
                     NEVLKVLLRSELYKDWLLQFNVGTSYPVIKDNDILNLPIPKISGEIQ"
BASE COUNT          238 a          140 c          118 g          301 t
ORIGIN      
        1 ttggatttct ccacttattt taggaattgg taaattcaaa atatcgttat ctttaattac
       61 aggataactc gtacctacat taaattgtag taaccaatct ttatatagtt ctgaccgtaa
      121 tagcaccttt aaaacttcat tattaaaaac agagttttcc tttctgcgta atactgtaaa
      181 cgcaccacta acaactaaat cttgatcatt ttcaccgata attgttactg caccacgata
      241 agggcgaacg gttgaaatta aaatatcacc tgtttgggct tgagttttag cattagcagg
      301 taattcttgt gtctctatcc aattcgtttg atatgaacca tcaccaatat taacatcacc
      361 aatttcaata tattgataca tcggttttgt ccaatcaggt ttttgggtga tatgtaaata
      421 ttcatcacga ataaaaacat aatcttgcga ttgaattaaa ttccagtatt gctcatattt
      481 agtttgataa tactctgcat ctaatcgtcc tgtttgtaag aaactctctt ttaaggattt
      541 aacatttaca ggattgctat cagcttgaaa agctctcaaa ccgagatttt ctaataataa
      601 agtttcagct tcttggtaac tttgtgttga tttttgtaca aaatcattac tttgcttaat
      661 gattttatct acctgctttt gtgtttctag atcaattaag gggactttaa tcgcaccaag
      721 ctcctcatta ttcagattgt attgtcctgt tgggcgtgca ttccggttaa tttggatttt
      781 tccataagaa ctttgta
//
LOCUS       DANLZC010000258          794 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.262, whole genome shotgun sequence.
ACCESSION   DANLZC010000258 DANLZC010000000
VERSION     DANLZC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.262"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            62..733
                     /locus_tag="PXE44_002570"
     CDS             62..733
                     /locus_tag="PXE44_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HDL5590018.1"
                     /translation="MKKSTLAIILGGALLTGTAVAHQAGDVIFRAGAVHVKANSSSDA
                     KSKAATGIDVDLEVKNNTQLGLTATYMLADNVGIELLGATPFSHKINAKAKVLESGAE
                     VDLDRVVILKQLPPSLYAQYYFFELTAKVKPYVGAGLNYTRFYHAKSVSPAVTDLSVK
                     KHSFGPVANLGVDVKLTDNIYFNAAAWYTRIKTTAKFKAAGADHEVKVKLDPVVLFAG
                     FGVKF"
BASE COUNT          250 a          136 c          162 g          246 t
ORIGIN      
        1 tttacacttc gctaaagttt acctcattat aggtattcta attatttttg aggatatgaa
       61 aatgaaaaaa tcaactttag caattatttt aggtggggca ttattaactg gaactgcggt
      121 ggcacatcaa gcaggtgatg tgatattccg tgccggtgcg gtacatgtta aggcaaattc
      181 aagttcagat gcaaaatcaa aagcagcgac aggtattgat gtagatttag aagtgaaaaa
      241 caatactcag ttaggcttaa ctgcaactta tatgttagcc gataatgtgg gtattgagtt
      301 attaggtgca acccctttct ctcataaaat taatgccaaa gccaaagttt tagaatcagg
      361 tgcagaagta gatttggata gagttgttat cttaaaacaa ttaccaccga gcctttatgc
      421 ccaatactat ttctttgagc taactgcaaa agtaaaacct tacgttggtg caggtttaaa
      481 ttacactcgc ttctaccacg ctaaatcagt tagccctgct gttactgact taagtgtaaa
      541 aaaacattct ttcggaccgg tagcaaacct aggtgttgat gtaaaattaa ccgataacat
      601 ctacttcaac gcggcagctt ggtacacacg cattaaaaca accgcaaaat ttaaagcagc
      661 aggtgcagat cacgaagtaa aagtaaaact tgatccggtt gtcttatttg ccggtttcgg
      721 ggtgaagttc taattatttg ttgggaacga gttgggctaa tcttagtgat tagtcctttt
      781 ttgttaaagt tgtt
//
LOCUS       DANLZC010000259          735 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.263, whole genome shotgun sequence.
ACCESSION   DANLZC010000259 DANLZC010000000
VERSION     DANLZC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.263"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            28..546
                     /locus_tag="PXE44_002571"
     CDS             28..546
                     /locus_tag="PXE44_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein"
                     /protein_id="HDL5590019.1"
                     /translation="MQKKLFIAQIKQNLSELNVFSTDNIFLNSPYFSQQTGLVSVFIA
                     EIEKTVELLLNQTEVLYSEFYAEKLVKQVDALKNAVEKIQSKPESAQFHSSYQFSPNI
                     HRLAPNKRLQEYRKALRALNEKISWLVEQNLNTQNEATKQTLQNQITETEYRKMKCLK
                     AIEDLEQELLFK"
     gene            complement(550..623)
                     /locus_tag="PXE44_002572"
     tRNA            complement(550..623)
                     /locus_tag="PXE44_002572"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(589..591),aa:Cys,seq:gca)
     gene            complement(639..714)
                     /locus_tag="PXE44_002573"
     tRNA            complement(639..714)
                     /locus_tag="PXE44_002573"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(679..681),aa:Gly,seq:gcc)
BASE COUNT          262 a          145 c          131 g          197 t
ORIGIN      
        1 aaattttctt tattgataac atcaaccatg caaaaaaaac tttttatcgc ccaaataaaa
       61 caaaacctct cggaattaaa tgtattttca acggataata tttttctgaa ttcaccctat
      121 ttctcacagc aaaccggttt ggtgagtgtg ttcattgctg aaattgaaaa aacagttgag
      181 ctattattga accaaactga ggtgttatat agtgaatttt acgctgaaaa attggttaaa
      241 caagttgatg cattaaagaa tgccgtagaa aaaatccaaa gtaagccaga atcagctcaa
      301 ttccattctt cctatcaatt ttctccaaat attcatcgtt tagcgcccaa caaacgctta
      361 caagaatacc gtaaagcgtt acgtgcattg aacgagaaaa ttagctggtt agttgagcaa
      421 aatttaaata cgcaaaatga agcaactaaa caaaccctgc aaaatcaaat tacagagaca
      481 gaatatcgca aaatgaaatg tttaaaagca attgaagatt tagaacaaga gttgctattt
      541 aaatagtgat ggaggcgtgt cccggagtcg aaccgagcta catggatttg caatccagtg
      601 cataaccgct ttgctaacac gccataagat ttggaaattg gagcgggaaa cgaggctcga
      661 actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac tgagctattc ccgcatctga
      721 attcccacaa cattg
//
LOCUS       DANLZC010000260          704 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.264, whole genome shotgun sequence.
ACCESSION   DANLZC010000260 DANLZC010000000
VERSION     DANLZC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.264"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..106
                     /locus_tag="PXE44_002574"
     CDS             <1..106
                     /locus_tag="PXE44_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL5590020.1"
                     /translation="IEVVALRVNVDNPAYIESYELLEKLGVQIVDIRE"
     gene            108..>704
                     /locus_tag="PXE44_002575"
     CDS             108..>704
                     /locus_tag="PXE44_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL5590021.1"
                     /translation="MKKYLISLEKDVKRRELFFSQPNTEDFEVFSAINTMSSDWEALN
                     NHFDLALFEQRYGRKTTKGEVGCTLSHLAVYQKIAEDSTIAEDDYCLVCEDDALFADD
                     FQKHLDALLAQNLKVDIILVGQSKIPTFNDSELELNYPSTFGFLQKNIPNSQYKYAYS
                     YLNYFAGTVAYLIKKSTACQFLAQCAGKNPYWLADDFIL"
BASE COUNT          233 a          102 c          138 g          231 t
ORIGIN      
        1 tattgaagtg gtagcattaa gagtgaatgt agataatcct gcttatattg agagctatga
       61 attgcttgaa aaattaggcg ttcagattgt tgatattaga gaataaaatg aagaaatatt
      121 taatttcatt agaaaaagat gtaaaaagac gagagctatt ttttagccag cctaatacgg
      181 aagattttga ggtgttcagt gccattaata caatgagtag tgattgggag gcattaaata
      241 accattttga tttggctctg tttgaacagc gatatgggcg taaaaccact aagggcgaag
      301 taggttgtac tttaagccat ttagccgttt accaaaaaat tgccgaagat agcactattg
      361 cggaagatga ttactgccta gtctgtgaag atgatgcctt atttgccgat gatttccaaa
      421 aacatcttga tgctttatta gcacaaaatt taaaagtcga tattatttta gttgggcagt
      481 cgaaaattcc aacttttaac gatagcgaat tagagcttaa ttacccatca acctttggct
      541 ttttgcaaaa aaatattccg aatagccaat ataaatatgc ttattcttac ctgaattatt
      601 ttgctggtac ggttgcatat ttaattaaaa aatcgaccgc ttgtcagttt ttagcacaat
      661 gtgccggaaa aaatccatac tggcttgccg atgattttat ttta
//
LOCUS       DANLZC010000261          698 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.265, whole genome shotgun sequence.
ACCESSION   DANLZC010000261 DANLZC010000000
VERSION     DANLZC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.265"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..122)
                     /locus_tag="PXE44_002576"
     CDS             complement(<1..122)
                     /locus_tag="PXE44_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5590022.1"
                     /translation="MELEQQIIDYALQHEPHEMCGFVVFDGNKNRFIPCENQAE"
     gene            complement(126..>698)
                     /locus_tag="PXE44_002577"
     CDS             complement(126..>698)
                     /locus_tag="PXE44_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5590023.1"
                     /translation="LNELKTSIVWQGRTYDPYPIEATGFERNSSGPSNRPTLTLSNLF
                     GLVTGIANQFDECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLSSL
                     TRETATFTLALPIETDNAKIPSRIIMADTCTWIYRSAECGYTGKQYFDEKDKATSDPK
                     ADKCSHCLNGCELRGNQRNFGGFVSVNKLG"
BASE COUNT          175 a          158 c          157 g          208 t
ORIGIN      
        1 tcttccgctt ggttttcgca agggataaat cgatttttat tgccgtcaaa aacaacaaag
       61 ccgcacattt cgtgcggttc gtgttggagg gcgtagtcga ttatttgttg ttccaattcc
      121 attcattacc ccaatttatt caccgataca aatccaccaa aattacgctg attgcctcgc
      181 aattcacaac cgttcaggca atggctgcat ttatcggctt ttgggtctga tgttgccttg
      241 tctttttcat caaaatactg cttgccggtg tagccacatt ccgccgagcg gtaaatccaa
      301 gtgcaggtgt ccgccataat aattctgctg gggattttgg cattatcggt ttcaatcggc
      361 aacgcaagcg taaaagtagc agtctctcgt gtgaggcttg aaagttgctc aatcacaaaa
      421 tggctgatga tttcctgatt aggatcggct ttggcattac ctccagcaaa gtttactgca
      481 tctaaatatt gggcgtaaac ctgatgacgg cgaacaatcg caccgataca ttcgtcaaac
      541 tgattagcaa tacccgtaac taagccaaac agatttgata gcgtaagagt agggcgattg
      601 cttgggccgc tgctgttacg ctcaaatcct gttgcctcga tggggtaagg gtcgtaagtt
      661 cgaccttgcc agacaatact tgttttgagt tcatttag
//
LOCUS       DANLZC010000262          691 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.266, whole genome shotgun sequence.
ACCESSION   DANLZC010000262 DANLZC010000000
VERSION     DANLZC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.266"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..683
                     /gene="fkpA"
                     /locus_tag="PXE44_002578"
     CDS             <1..683
                     /gene="fkpA"
                     /locus_tag="PXE44_002578"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5590024.1"
                     /translation="LFSTNVMAEEKADPKFVDDSSYAVGVLMGKNINGVIESQKDIFT
                     YNQDKILAGVQDTLKKTGKLTDEDLQKQLKSLDAYLSAKEAKINEEKTKATVAAGDKF
                     RADYKKQSGVKETASGLLYKIEKAGEGNSPKAEDTVKVHYKGTLTDGTVFDSSYDRGE
                     PIEFQLNQLIPGWIEAIPMLKKGGKMEIVVPPALAYGDRAAGKIPANSTLKFEIELLD
                     FKAADAKK"
BASE COUNT          245 a          107 c          154 g          185 t
ORIGIN      
        1 cactttttag tacaaatgtg atggcagaag aaaaagcaga tcctaaattt gtggatgatt
       61 cctcttatgc cgtgggtgta ttaatgggta aaaatattaa tggtgtgatt gagtcacaaa
      121 aggatatttt tacctataac caggataaaa tcttagccgg tgttcaggac accttaaaga
      181 aaaccggtaa attaaccgat gaagatttgc aaaagcagtt aaaatcgtta gatgcctatt
      241 tatctgcgaa agaagcaaag attaacgaag aaaaaaccaa agcaacggta gctgcaggcg
      301 ataaattccg tgctgattat aaaaaacagt ctggcgtaaa agaaacggct tcaggcctgt
      361 tatataaaat tgaaaaagca ggtgaaggca attcaccaaa agcggaagat actgtgaagg
      421 tgcattacaa aggcactttg actgatggca cggtatttga cagctcttac gaccgtggtg
      481 agccgattga atttcagtta aatcaattaa ttccgggttg gattgaagcg attcctatgt
      541 tgaaaaaagg cggtaagatg gaaattgtag tgccacctgc attagcttat ggtgatcgtg
      601 ccgcaggcaa aattccagca aattcaacct tgaaatttga aattgaatta ttagatttta
      661 aagcagcaga tgctaagaaa taattcgtta t
//
LOCUS       DANLZC010000263          668 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.267, whole genome shotgun sequence.
ACCESSION   DANLZC010000263 DANLZC010000000
VERSION     DANLZC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.267"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            15..>668
                     /locus_tag="PXE44_002579"
     CDS             15..>668
                     /locus_tag="PXE44_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL5590025.1"
                     /translation="MTGQKVPSVTFRTRQNDAWVDVTTDELFNNKTVVVFSLPGAFTP
                     TCSSTHLPRYNELACEFKALGVDDIICMSVNDTFVMNAWKADQESENITVIPDGNGEF
                     TEGMGLLVDKNDLGFGKRSWRYSMLVKNGIVEKMFIEPQEPGDPFKVSDADTMIKYLS
                     PNWTAKESVSIITKPGCLFCAKAKALLKEQGYTFEEIVLGRDASTISVRAITGKTSVP
                     "
BASE COUNT          207 a          106 c          151 g          204 t
ORIGIN      
        1 tgtcttttaa agatatgacg ggtcagaaag tacccagtgt aacatttcgt acacgtcaaa
       61 acgatgcttg ggttgatgtc acaaccgatg agttatttaa taacaaaacg gtggttgtgt
      121 tttcattacc cggagcattt acaccgactt gttcatcaac acatttgcct cgctataatg
      181 aattagcttg tgaatttaag gcattagggg tagatgatat catctgtatg tcagttaatg
      241 atacctttgt aatgaacgct tggaaagcag atcaagaatc tgaaaatatt actgttattc
      301 ctgatggtaa tggtgagttc acagaaggta tggggctatt agttgataaa aatgaccttg
      361 ggtttggtaa acgttcttgg cgttattcga tgctggtgaa aaacggtatt gtggaaaaaa
      421 tgtttattga gccacaagaa ccgggcgatc cgtttaaagt atcagatgcc gatacaatga
      481 ttaaatattt aagtccaaat tggacagcta aagagtctgt ttcgattatc actaaaccgg
      541 gttgcctgtt ctgtgcaaaa gcaaaagcat tattaaaaga gcaaggctat acttttgagg
      601 agattgtgtt aggtcgtgat gcaagcacaa tttctgtacg agcaattaca ggtaaaacct
      661 ctgtgcca
//
LOCUS       DANLZC010000264          658 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.268, whole genome shotgun sequence.
ACCESSION   DANLZC010000264 DANLZC010000000
VERSION     DANLZC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.268"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            60..>658
                     /locus_tag="PXE44_002580"
     CDS             60..>658
                     /locus_tag="PXE44_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5590026.1"
                     /translation="MFTEGKIDFDKFQQLFSDNFSTEPDRYMLNWAGKSTAYQTLQTP
                     TSKTLTPCEEESVDFENTQNIFIEGENLDVLKALQKSYFNSVKMIYIDPPYNTGNDFI
                     YNDSFADTKADYAEKVGDVDEHGKLKRAFVRNSKENGHYHSNWLNMMLPRLHLAKNLL
                     RDDGVIFISIDDNEQAQLKLLCDEVFGEENFVAILSVEN"
BASE COUNT          217 a          128 c          126 g          187 t
ORIGIN      
        1 ttgccaactt ttcgcttaaa gatcaacaac tttctcaatt aaaatccctt ttacctgaaa
       61 ttttcaccga aggtaaaatc gacttcgaca aattccaaca acttttttcg gataattttt
      121 ccaccgaacc tgaccgctat atgctcaact gggcaggaaa atcaacagcc tatcaaacct
      181 tacaaacccc aaccagcaaa acgctcactc cttgcgagga agagagtgtg gattttgaaa
      241 atacgcagaa tatctttatt gagggtgaaa atttagatgt attaaaagcc ctgcaaaaat
      301 cctacttcaa ttcggtcaaa atgatttata tcgatccgcc atacaataca ggcaacgatt
      361 ttatttataa cgacagtttt gccgatacca aagcagatta tgcggagaaa gtgggcgacg
      421 ttgatgaaca cggcaaattg aaacgagcct ttgttcgtaa cagcaaagaa aatgggcatt
      481 atcacagtaa ctggctgaat atgatgttgc ctcgtttgca tctcgcaaaa aatttactgc
      541 gtgatgacgg ggtgattttt atttcaattg atgataacga acaggcacag ctgaaattgc
      601 tttgtgatga agtgtttggg gaggagaatt ttgttgccat attatcagtt gaaaataa
//
LOCUS       DANLZC010000265          651 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.269, whole genome shotgun sequence.
ACCESSION   DANLZC010000265 DANLZC010000000
VERSION     DANLZC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.269"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(189..>651)
                     /locus_tag="PXE44_002581"
     CDS             complement(189..>651)
                     /locus_tag="PXE44_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590027.1"
                     /translation="GDRNYQLYIDLATGELVSKLGKGLTTREFDAAVKAFENAFNAKD
                     TAENTSPAESKETEQENKQETTKLVITKPTLKGSEKQIAWADEIINLFIAKIGDTSIP
                     VTATQEQITQAQAMVDNFFAMTNAGKWIEKFKGLNDSSEPKQFWLSVKYGW"
BASE COUNT          146 a          132 c          118 g          255 t
ORIGIN      
        1 cctcttatag ggtatctcaa gattattgtc tgtacgtttg ttggcacatc gtcaccataa
       61 gtagcagcga caatttgccc gataatgcgc ataatttgtg gtgttacttg tgtagtcata
      121 ataatctcca gttatctgtt tgttttagcc gtaagccttt gctcttacgg cttatttgtt
      181 atctcaatct accatccata ttttacggat aaccaaaatt gctttggttc gctactatca
      241 tttagacctt taaatttttc gatccacttt ccagcgtttg tcattgcaaa aaaattatct
      301 accattgctt gagcttgagt aatttgttct tgcgttgctg ttactgggat agaggtatca
      361 ccaatcttcg caataaaaag attgatgatt tcgtctgccc aagcgatttg tttttcagag
      421 ccttttaatg ttggttttgt gattacaagc ttagttgttt cttgtttgtt ctcttgctct
      481 gtttctttgc tttctgctgg cgatgtattt tcagcggtgt ctttagcatt aaaagcgttc
      541 tcaaatgctt ttacggcagc gtcgaactct cttgttgtta atcctttgcc aagtttgctc
      601 actaactcac ctgtggctaa gtcaatgtac aactgataat tccgatcacc t
//
LOCUS       DANLZC010000266          628 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.270, whole genome shotgun sequence.
ACCESSION   DANLZC010000266 DANLZC010000000
VERSION     DANLZC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.270"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
BASE COUNT          199 a           90 c          108 g          231 t
ORIGIN      
        1 attaatgatt aatcccttct tgtttggata gttactctat ttttgaagaa cattccgtat
       61 ttttaatgat gttttataag caggatataa aatttttaat atttcaaata aaaagtgagc
      121 taaaaaagat tttaactcac tttcatttcg taggctcaaa ttagacctac tacgccgata
      181 taaaaagttg gtctttttat atcaaagcta agctcttaac aacgagtagt agattctcta
      241 atatgtttag ctgtaacttt cattggcttt tgttgcgctg tagtgttttt catattgttc
      301 tccagatttt gtgagtaatt agtaagacct tttcttttga aaaggaccga gctttcaaag
      361 tggtctgatt accttaccta ttgagcaaag ccatgtcatt atatcataac cattgttttt
      421 agtcaatacc attttgggga atgcatggaa ggcgagtaca aattgaaggc tgatgtcacg
      481 atctatcatg atacgcctta tacaaaagtt ctctttggaa gtacatatat tgagatttta
      541 gacgatgacc agtattactt ttcgatttta gaaaaacgaa gatggaagtt agaaaattta
      601 cctgatgaat tagttgatgt actaaaag
//
LOCUS       DANLZC010000267          621 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.271, whole genome shotgun sequence.
ACCESSION   DANLZC010000267 DANLZC010000000
VERSION     DANLZC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.271"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..>621
                     /locus_tag="PXE44_002582"
     CDS             <1..>621
                     /locus_tag="PXE44_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HDL5590028.1"
                     /translation="FLTGEAKRKDKDAREKTSGLVDIGLITEDRLITDAGRELLKVTS
                     SGDFETNNVFNINRDSFIYLKQLLKTSIDVSGSIVRPFIAVVKCLTELEFLSYDEFTY
                     FVPLIRDDESAKQIISDIKLYREGKILPEEIIYKRLMQMENYKLAQEEFIASDVDENL
                     ICLVGMNRKSRNYDKPYYKLYENLKKVFLDGENIYESLLNSAKNIN"
BASE COUNT          241 a           72 c          125 g          183 t
ORIGIN      
        1 tttcctaaca ggagaagcaa aaagaaaaga caaagatgcc agagaaaaaa catcaggatt
       61 ggttgatatt ggtttaatta cagaagatag attgattacg gatgcaggaa gagaactcct
      121 taaagtaaca agtagtgggg attttgaaac caataatgtg tttaatatta atagagatag
      181 ctttatctat ctaaaacagc ttcttaaaac gagtatagat gtaagtggta gtatcgttag
      241 accgtttatt gcggttgtaa agtgcctgac ggaacttgag tttttaagtt atgacgagtt
      301 tacttatttt gttccgctaa ttagagatga cgaaagtgca aaacaaatca tatcggatat
      361 taaattatat cgagaaggta aaattttacc ggaagaaata atttataaga ggcttatgca
      421 aatggaaaac tacaaacttg cacaagaaga atttatagcc tctgatgttg atgaaaatct
      481 aatatgttta gtgggaatga atcgtaaaag cagaaactat gacaagccgt actataaatt
      541 gtatgagaat ttaaagaagg tatttcttga tggcgaaaac atttatgaat cgctattaaa
      601 ttctgcaaag aatataaatc a
//
LOCUS       DANLZC010000268          614 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.272, whole genome shotgun sequence.
ACCESSION   DANLZC010000268 DANLZC010000000
VERSION     DANLZC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.272"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..>614
                     /locus_tag="PXE44_002583"
     CDS             <1..>614
                     /locus_tag="PXE44_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL5590029.1"
                     /translation="NTVLNTLQCDYVLFSSEDNYQVIINLKKADYPKNEANFITMTLN
                     KKFGDAKFSGANHYLRCASFFNKKSTNNNEKSVLVDFTNTKTEEDNKCYFDNLLSSYK
                     NNNVKLEPLDIKIIDELGDDKAVIAQKEIQAEIALCKRIFKQLDWSAVDFRIVKRLYR
                     KGFSENEIAVALVRFTDFEDRHCDSHDYLTRTITKAIQNYQQCS"
BASE COUNT          243 a           96 c           93 g          182 t
ORIGIN      
        1 taaacacagt tttaaacaca ctacaatgcg attatgtgct gttttcttct gaagacaact
       61 accaagtcat catcaatcta aaaaaagctg attaccccaa aaatgaagca aatttcatta
      121 caatgacttt aaacaagaaa tttggtgatg caaaattttc cggtgcaaac cattacttaa
      181 gatgtgccag cttcttcaac aagaaaagca caaacaacaa tgaaaaatca gttttagttg
      241 attttacaaa cacaaagaca gaagaagaca ataaatgtta ttttgataac ttattatcta
      301 gttataaaaa taacaatgta aaacttgagc ctttagacat caaaattatt gatgagcttg
      361 gagatgataa agcagttatt gcacaaaaag aaattcaagc agaaattgct ttgtgtaaac
      421 gtatattcaa acaattagat tggtctgctg ttgattttag aattgttaag cgtctgtaca
      481 ggaaaggatt ttcagaaaat gaaattgctg ttgcacttgt cagatttact gattttgaag
      541 acagacattg tgattcacat gattatttga caagaacaat cacaaaagcc attcaaaact
      601 atcaacaatg ctca
//
LOCUS       DANLZC010000269          604 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.273, whole genome shotgun sequence.
ACCESSION   DANLZC010000269 DANLZC010000000
VERSION     DANLZC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.273"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(17..>604)
                     /locus_tag="PXE44_002584"
     CDS             complement(17..>604)
                     /locus_tag="PXE44_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5590030.1"
                     /translation="TYSSEQLHKIDYILHNNIILPSFLKPSWFILAIIMTLRCTGIRR
                     GQLVKLKIGDVNLSNRTIYISPEINKNHDYHIVPISDSLYPHIERLIQELKIHKQTDD
                     CQLFNLNLFSRYTRRKGQPMSQNQLTHIFRHISDLVGFTSSPHRFRHTVATQLMKKPE
                     NVYVVKKLLGHKDISVTLGYIEYDVEMLRDTVNLL"
BASE COUNT          189 a          104 c          100 g          211 t
ORIGIN      
        1 tctcatttct cttttattag agtaagttca cggtgtctcg tagcatttcc acatcgtatt
       61 caatatatcc taatgtaacg ctgatatctt tatgacctaa taatttctta actacataaa
      121 cattttcagg tttcttcatc aattgagtag ctactgtatg ccgaaatcta tgtggtgatg
      181 acgtgaagcc aaccaaatcg gaaatatgcc taaatatatg agtaagctgg ttttggctca
      241 tcggctgacc tttcctacga gtataacgag aaaataaatt gaggttaaat aactggcaat
      301 catccgtttg tttatggatc tttaactcct gaatcagcct ttctatatgg ggataaagtg
      361 aatcggatat cggtactata tgataatcat ggtttttatt aatttctggt gaaatataga
      421 tcgtcctatt tgataaatta acatcaccga ttttcagttt aaccaattgc cctctcctaa
      481 ttcctgtgca tctcaatgtc ataatgatag caagaataaa ccagctaggt tttaaaaaac
      541 taggtaagat gatattatta tgtagaatat aatcgatttt atgcaactgc tcactggaat
      601 aagt
//
LOCUS       DANLZC010000270          575 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.274, whole genome shotgun sequence.
ACCESSION   DANLZC010000270 DANLZC010000000
VERSION     DANLZC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.274"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..296
                     /locus_tag="PXE44_002585"
     CDS             <1..296
                     /locus_tag="PXE44_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590031.1"
                     /translation="DTRYNKNGQGAVSERNIIDAKNRFKASHPYAKIISCVRGSETYV
                     TRPAKANATTKSVKEKQSQPQESSSLSSMLLGAAVTAGVGFVANKWFSKKDNT"
     gene            324..>575
                     /locus_tag="PXE44_002586"
     CDS             324..>575
                     /locus_tag="PXE44_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590032.1"
                     /translation="MAGLLPPYFKKKNSDHYKDAVKQFGKEQVDEVIAQCDELESWVK
                     EMPTKQLIMYVGCFHTFNKLSQSKAYVQENFPNVLENPFL"
BASE COUNT          210 a           93 c          107 g          165 t
ORIGIN      
        1 cagataccag atataacaag aatgggcaag gtgctgtttc tgaaagaaat attatagatg
       61 ctaaaaatcg ttttaaagcg agccatccat acgcaaaaat tatttcttgt gttagagggt
      121 ctgagacata tgtaacacgt ccagcaaaag ctaatgctac aacaaaatct gtcaaggaaa
      181 agcaatctca accacaagaa agttcatcgc tcagttcaat gttacttggt gcggctgtaa
      241 ctgctggggt tggttttgta gccaataaat ggtttagcaa aaaagataac acttaattta
      301 tctctaacca atgtggagta aaaatggctg gattattacc accttatttc aaaaagaaaa
      361 atagtgatca ctataaagat gctgtaaaac aatttgggaa agaacaagtt gatgaggtta
      421 tagcacagtg tgatgaactt gaatcttggg taaaagaaat gccaacaaaa caacttatta
      481 tgtatgttgg ttgtttccat acttttaaca aattatctca atctaaagct tatgttcaag
      541 agaactttcc aaacgtactg gaaaacccgt ttctt
//
LOCUS       DANLZC010000271          573 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.275, whole genome shotgun sequence.
ACCESSION   DANLZC010000271 DANLZC010000000
VERSION     DANLZC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.275"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            118..543
                     /locus_tag="PXE44_002587"
     CDS             118..543
                     /locus_tag="PXE44_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL5590033.1"
                     /translation="MYKHILVAVDLSEESLVLVRKGAALAEKCGAKLSLIHVDVNFSD
                     LYTGLIDINMSSVQYSVVEETNTALAELASKVNYPVSECLNGTGDFSQVLEEAVEKHN
                     VDLLITGHHQDFWSKFMSSTRRVMNNITVDMLVVPLADE"
BASE COUNT          189 a           87 c          114 g          183 t
ORIGIN      
        1 gtatgaaaaa tataatcaaa tttttatttt tgagatctac ttcaagtttt tatcaaaaag
       61 agtgtgttag tatgtttata cacaaagtaa acaactctca caagcaagga gcatattatg
      121 tataaacata tcttagttgc agtggatctt tctgaagaaa gtcttgtttt agtgcgtaag
      181 ggggctgcgt tagcagaaaa atgcggagct aaactttccc tcattcacgt tgatgttaat
      241 ttttcggatc tctatacagg attaattgat attaatatgt cttcggtaca atacagtgtt
      301 gttgaagaaa caaatacggc actggctgaa cttgcctcaa aagtaaatta tcctgtatct
      361 gaatgtttaa acggaacggg agattttagt caagttttgg aagaggccgt agaaaaacac
      421 aatgtcgatt tactcatcac tggccatcat caggacttct ggagtaaatt tatgtcttca
      481 actcgtcgag tcatgaataa tatcactgtt gatatgctgg ttgtgccatt agcagatgaa
      541 tagcaagaaa gtgaatgaaa aataaccgct tga
//
LOCUS       DANLZC010000272          565 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.276, whole genome shotgun sequence.
ACCESSION   DANLZC010000272 DANLZC010000000
VERSION     DANLZC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.276"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(19..303)
                     /locus_tag="PXE44_002588"
     CDS             complement(19..303)
                     /locus_tag="PXE44_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010128390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590034.1"
                     /translation="MANITLSHITPKHFFETVRLLKMQEHRIYIGVDSIEVRFKQKRF
                     ESKKETDKRLEQLEHFMLFLQEIINSDIPIDLDEFIYTYHRSVRRGFYRR"
     gene            complement(321..482)
                     /locus_tag="PXE44_002589"
     CDS             complement(321..482)
                     /locus_tag="PXE44_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL5590035.1"
                     /translation="MEKIENSSNLVLKLSLENQMLILESLEHPKEPNLVMLEALKKYS
                     LYVAKTSNK"
BASE COUNT          172 a          100 c           90 g          203 t
ORIGIN      
        1 cactcctctt tcacatcact aacgtcgata aaaacctctc cttactgacc tgtgataggt
       61 gtatataaac tcatctaaat ctataggaat atctgaatta atgatttctt gtagaaagag
      121 cataaaatgc tctaactgtt ctagtctctt atctgtttcc ttttttgact caaaacgctt
      181 ttgcttaaaa cgaacttcta tcgaatctac accgatgtaa attcgatgtt cctgcatttt
      241 gagtagtcgg acagtttcaa aaaaatgttt aggggtgatg tgagaaaggg ttatatttgc
      301 catagtttag cttgaaaacg ctatttatta cttgttttag caacatacaa agaatacttt
      361 tttaaagcct ctagcattac taaattaggc tctttaggat gttctaagct ctcaagaatt
      421 agcatttgat tttcaagaga aagctttaga acaagatttg aactattctc tattttttcc
      481 attttaatta tctcattaga tgactttcgc ataacaacag gttatgtgta aattctcgtg
      541 cagtgggaca agcccgattg tactg
//
LOCUS       DANLZC010000273          550 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.277, whole genome shotgun sequence.
ACCESSION   DANLZC010000273 DANLZC010000000
VERSION     DANLZC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.277"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..267
                     /locus_tag="PXE44_002590"
     CDS             <1..267
                     /locus_tag="PXE44_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590036.1"
                     /translation="ENLYGFLRDGATLVANGIVNEPSVDCFSQEIAQFTGCHIFSSLY
                     IAFNTQRSFKSHWDSRDIFAVQMQGKKRWIIHTPTFKKTIVYAS"
     gene            257..>550
                     /locus_tag="PXE44_002591"
     CDS             257..>550
                     /locus_tag="PXE44_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HDL5590037.1"
                     /translation="MHRSKDMPEYAPNLDDVYMDIVLEAGDVLYLPRGWWHDPIPVGE
                     ETVHLAVGIFPAYTHNYLTWVSQNMVEKEIARASLSHYESDKELIAQLAEQTAE"
BASE COUNT          169 a           83 c          112 g          186 t
ORIGIN      
        1 gaaaatttat atggtttttt aagagatggg gcaacattag tcgcaaatgg tattgtaaat
       61 gaaccaagtg ttgattgctt ctctcaagag atagctcaat ttacaggctg tcatattttt
      121 tcaagtttat atattgcgtt taatactcaa agatctttta aaagccattg ggatagtcgt
      181 gatatttttg cagttcaaat gcagggtaaa aagcgttgga ttattcacac tccaacattt
      241 aaaaaaacca ttgtttatgc atcgtagtaa agatatgcct gaatatgctc ctaatcttga
      301 tgatgtatat atggatattg tattagaagc tggagatgtt ctttatttac ctcgtggctg
      361 gtggcacgat cctattcctg ttggagaaga aactgtgcat ttagctgtag gcatttttcc
      421 tgcttatact cataactatc tgacttgggt atctcaaaat atggttgaga aagaaattgc
      481 ccgagcttct ttatcgcatt atgagtcaga taaagagttg atagcacagt tggcagaaca
      541 aacagcagaa
//
LOCUS       DANLZC010000274          544 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.278, whole genome shotgun sequence.
ACCESSION   DANLZC010000274 DANLZC010000000
VERSION     DANLZC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.278"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..>544
                     /locus_tag="PXE44_002592"
     CDS             <1..>544
                     /locus_tag="PXE44_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590038.1"
                     /translation="ANVIAKLQLEEDKYSLDYMTVKRAICDELIVDIAETDAKKFLDY
                     LIKENVISKNAYYNRDEEYIYFNYERIGDYQKANLILKNIHNQDDFVQWLSTEQGKKL
                     YSRVGYDKGVWEALSVLLPERFDFEVFEVIDYSKEWSWFEIIVENLFWRQPEKINMQK
                     LHQFFKNSPYFNQATWINILY"
BASE COUNT          210 a           52 c          101 g          181 t
ORIGIN      
        1 gctaatgtta ttgccaaatt acaattggaa gaagataaat atagtttgga ttatatgacg
       61 gttaagagag caatttgtga tgaattaata gtagatattg cagaaacaga tgccaaaaaa
      121 tttcttgatt atttaatcaa agaaaatgtt attagtaaaa atgcttacta taacagagat
      181 gaagagtata tttattttaa ttatgaacga attggggatt atcaaaaggc caatttaata
      241 ttaaaaaata ttcataatca agacgatttt gttcaatggt taagcactga acaaggaaaa
      301 aagctatata gtagagttgg ctatgataag ggcgtatggg aagcattaag tgtattgttg
      361 ccagaacgat ttgattttga agtatttgaa gtgattgatt attcaaagga atggtcttgg
      421 tttgagatta ttgtagaaaa tctcttttgg aggcagcctg aaaaaatcaa tatgcaaaaa
      481 ctacatcaat ttttcaaaaa tagtccatat tttaatcaag caacttggat taatatttta
      541 tatc
//
LOCUS       DANLZC010000275          509 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.279, whole genome shotgun sequence.
ACCESSION   DANLZC010000275 DANLZC010000000
VERSION     DANLZC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.279"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            60..479
                     /locus_tag="PXE44_002593"
     CDS             60..479
                     /locus_tag="PXE44_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor domain-containing protein"
                     /protein_id="HDL5590039.1"
                     /translation="MVPSNEAVLKAVHDTLVISTTDLQGVITYANDLFCKLTGFTREE
                     LIGQPHNIVRHPAVPKEVYKDMWDTIQAGKIWTGIVPNCGKGGVVYVVDTTVQPMFDE
                     TGKITGYISVRRVINDLMTNFEAVESAKEIFDDFYGE"
BASE COUNT          180 a           91 c           94 g          144 t
ORIGIN      
        1 actgataaac acataacaaa attcatttat attaatggag attcaacacg gctgaacaaa
       61 ttgttccttc aaatgaagct gttttaaaag cagtccacga tactttagtt atttcaacaa
      121 ctgatttaca aggtgttatt acttacgcta acgacctttt ctgcaaatta accggtttta
      181 cccgtgaaga attaattgga caaccgcata atatcgtacg ccacccagct gtaccaaaag
      241 aagtttataa agatatgtgg gatacaatcc aagccggtaa aatctggaca ggtatcgtac
      301 caaactgcgg taaaggcggt gtagtttatg tggttgatac caccgttcaa ccaatgtttg
      361 atgaaacagg caaaattaca ggctatatca gcgttcgccg tgtaattaat gatttaatga
      421 ccaactttga agcagtagaa tcagcaaaag aaatctttga tgacttctac ggtgaataat
      481 taaaaaacaa tgattgcgtg taaaagatg
//
LOCUS       DANLZC010000276          504 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.280, whole genome shotgun sequence.
ACCESSION   DANLZC010000276 DANLZC010000000
VERSION     DANLZC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.280"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            208..>504
                     /locus_tag="PXE44_002594"
     CDS             208..>504
                     /locus_tag="PXE44_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590040.1"
                     /translation="MSKLNKLLSRKTSRKIIPEQVNFDINLPIYIPLFLSDKTAKIQA
                     VWELEQENGERDAGAVKRNRIQFRYLPEGVHKLTLIVGTPLLSKGSKVYQCLIKV"
BASE COUNT          175 a           66 c           91 g          172 t
ORIGIN      
        1 aaatagggaa atgctcacaa tttatgcatt ccaattgtgg tctaatcaat aaattttgcc
       61 gatatatcga taggaagtag atgtaattac ctaaagtttg atctaggtta gttaaatggg
      121 aggtttttaa ctattatttc taatactttt attttaagct ttacagtaat gaatgagttt
      181 attttacatt ttacaaagga acattctatg tctaagttaa acaaattatt atcgagaaaa
      241 acatcgcgta aaattattcc tgagcaagtt aattttgaca ttaacttgcc tatctatatc
      301 ccgctgtttt tatcagataa gacagcgaaa attcaagcag tatgggagtt agaacaagaa
      361 aatggtgagc gtgatgctgg cgctgtaaag cgaaatcgta ttcagtttag atacttgcca
      421 gaaggggtac ataaattaac attaattgtt ggtacgcctt tattatcaaa aggaagtaag
      481 gtttatcaat gcctaattaa agtt
//
LOCUS       DANLZC010000277          490 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.281, whole genome shotgun sequence.
ACCESSION   DANLZC010000277 DANLZC010000000
VERSION     DANLZC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.281"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
BASE COUNT          162 a           75 c          101 g          152 t
ORIGIN      
        1 gtgatcagaa ttagattaaa gggagaatga aatgaaagaa ccatcaattt tacaattaag
       61 cttagtagtt ggagggaatt ctgaaaataa ggctaatcaa ccagatcttg tatcttatgg
      121 cgttgctttt gctacagggt ttgcaagagg ggggctttag gcggtttatc cagtacttct
      181 gttaatttag cccttgattt agcgaggagc ctacctatta caatacatga aaattcttta
      241 cctagcggtg taaaagaatt aattccatca tctttaccaa taaagcctac gtttggagtt
      301 ccaggtagaa cgaataataa ccaatctggc tcaaactacg attgtggtga tggtacagat
      361 tatcaaggct agtggagatg aaaatgaaaa ttcaatttcc aatttcttat caagaatttc
      421 gtgaaagtta ttctgagaaa caaccacttt taatgaaagg tgctatagag tagcaagatt
      481 tactttcttg
//
LOCUS       DANLZC010000278          480 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.282, whole genome shotgun sequence.
ACCESSION   DANLZC010000278 DANLZC010000000
VERSION     DANLZC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.282"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..>480)
                     /locus_tag="PXE44_002595"
     CDS             complement(<1..>480)
                     /locus_tag="PXE44_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL5590041.1"
                     /translation="EYIARMDADDISLPTRLSEQILYLEENHLDLCGCNALLFSEHGN
                     LGLTNKLNSHEALEKLMVAGDIGIIHPTFLARRKVFVESGGYNPLALHAEDMEFIAHI
                     LSMGFRLGNNPKVLFKYRFSTNSVTRRNAFHLYTTISNINKAYKSFKNGGDYIFVNSI
                     "
BASE COUNT          168 a           90 c           57 g          165 t
ORIGIN      
        1 tattgaattt acaaatatat aatcgcctcc atttttaaaa ctcttatatg ctttatttat
       61 attacttatt gtggtatata aatgaaaagc atttcttcta gtaacgctat ttgtagaaaa
      121 cctatatttg aataatacct taggattatt ccctaatcta aatcccattg ataaaatatg
      181 ggcaataaat tccatatctt ccgcatgtaa agctaaggga ttataccctc cagattcaac
      241 aaatactttt ctccttgcta aaaaagtagg gtgaattatt ccaatatcac cagccaccat
      301 aagcttttct aaagcctcat gactattcaa cttatttgta agaccaaggt tgccatgttc
      361 agaaaaaagc aaagcattac aaccgcataa gtctaaatgg ttttcttcta aatataaaat
      421 ttgctctgac aatctagtag gtaaagatat atcatcagca tccattctcg ctatatattc
//
LOCUS       DANLZC010000279          469 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.283, whole genome shotgun sequence.
ACCESSION   DANLZC010000279 DANLZC010000000
VERSION     DANLZC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.283"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..428
                     /locus_tag="PXE44_002596"
     CDS             <1..428
                     /locus_tag="PXE44_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590042.1"
                     /translation="KNAEKEFKKNYNKSLRYNAKKAQQRVQKYVEKVNQKNPQALKDL
                     DNISNLKKINENLKKAAKFTGIISSIKDYYDLAEGFRNGIKSNDWEPFLKTSAQIGAS
                     ALMSMLVASMITGPTTIGSIIISAAFISIGGYIISLASR"
BASE COUNT          186 a           67 c           79 g          137 t
ORIGIN      
        1 ttaaaaatgc agaaaaagaa tttaaaaaga attataataa aagtctaaga tataatgcta
       61 aaaaagcaca gcagagggtg caaaaatatg tcgaaaaagt caaccaaaag aatcctcagg
      121 cactaaaaga tctcgataac atatccaact taaaaaaaat taatgagaat ttaaaaaaag
      181 cagcaaaatt tactggtatt attagcagta ttaaggatta ttacgatcta gcagaagggt
      241 ttagaaatgg cataaagtct aatgattggg aacctttttt aaagacatct gctcagatag
      301 gtgcttcagc tttaatgagt atgttagtag cctcaatgat aacaggccca acgacaattg
      361 ggagtattat tattagtgct gcatttattt ctataggggg atatattatt tcactagctt
      421 cccgttagat agtctttgtt atactcaata acctactata ctacctata
//
LOCUS       DANLZC010000280          465 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.284, whole genome shotgun sequence.
ACCESSION   DANLZC010000280 DANLZC010000000
VERSION     DANLZC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.284"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(116..191)
                     /locus_tag="PXE44_002597"
     tRNA            complement(116..191)
                     /locus_tag="PXE44_002597"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Thr,seq:ggt)
     gene            complement(195..269)
                     /locus_tag="PXE44_002598"
     tRNA            complement(195..269)
                     /locus_tag="PXE44_002598"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Gly,seq:tcc)
     gene            complement(313..397)
                     /locus_tag="PXE44_002599"
     tRNA            complement(313..397)
                     /locus_tag="PXE44_002599"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Tyr,seq:gta)
BASE COUNT          144 a          107 c          107 g          107 t
ORIGIN      
        1 tttctaaaag cctcttaaac aaaaacggtt acaatggaac aaaagaaccc attaaccata
       61 atgtagaaat taataccaat aaaaatttgc gtgagaaaga tagaattcag agaagtggtg
      121 ctgataggcg gatttgaacc gccgacctca cccttaccaa gggtgcgctc taccaactga
      181 gctatatcag cgcttggagc gggcagcggg aatcgaaccc gcatcattag cttggaaggc
      241 taaggtaata gccattatac gatgcccgct gttctaaatt cgtctgacct acgcgattca
      301 aaatatcaga gatggtggag ggagaaggat tcgaaccttc gaaggctgag ccaacagatt
      361 tacagtctgc cccctttggc cgctcgggaa cccctccacg ataattgaat acttatttac
      421 gcctgagtta atggtgccga ctaccggaat cgaactggtg accta
//
LOCUS       DANLZC010000281          464 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.285, whole genome shotgun sequence.
ACCESSION   DANLZC010000281 DANLZC010000000
VERSION     DANLZC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.285"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            30..302
                     /locus_tag="PXE44_002600"
     CDS             30..302
                     /locus_tag="PXE44_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL5590043.1"
                     /translation="MKKGIHPENYREVLFYDASVQQGWVIRSCAATNKTMVWEDGKEY
                     PLYTLDTSSASHPVYTGKRREVNTEGRASQFKDRFKGFASTLSNQK"
     gene            311..436
                     /gene="ykgO"
                     /locus_tag="PXE44_002601"
     CDS             311..436
                     /gene="ykgO"
                     /locus_tag="PXE44_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDL5590044.1"
                     /translation="MKILNSLKTAKSRHPDCQIVRRKGKLYVICKTNPRFKARQR"
BASE COUNT          166 a           95 c           86 g          117 t
ORIGIN      
        1 aatgacctta tctaaattct ggagaaaata tgaaaaaagg gattcatcct gaaaattatc
       61 gtgaagtgtt gttctatgat gcctcagtgc aacaaggctg ggttatccgt tcttgtgcgg
      121 caaccaacaa aacaatggta tgggaagatg gcaaagaata cccactttac accttagata
      181 cctcatcggc gtctcaccct gtttacacag gtaaacgccg tgaagtgaat acagaaggtc
      241 gtgcaagtca atttaaagat agatttaaag gctttgcttc aacgctttca aatcaaaaat
      301 agaggataaa atgaaaatct taaactcact taaaaccgca aaatcacgcc acccggattg
      361 ccaaattgtc agacgcaaag gcaaacttta tgtgatttgc aaaaccaacc cgagatttaa
      421 agctcgtcag cgttaaaaac cagcacctta ataagctaat ttat
//
LOCUS       DANLZC010000282          462 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.286, whole genome shotgun sequence.
ACCESSION   DANLZC010000282 DANLZC010000000
VERSION     DANLZC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.286"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..>462
                     /locus_tag="PXE44_002602"
     CDS             <1..>462
                     /locus_tag="PXE44_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5590045.1"
                     /translation="IANYFAFDQKIQLNTQTNQTLEAIAQAIFKSWFVDFDPVRAKAA
                     ALSEGKSEHEANLAAMSVICGKDTSELNDTEYKALWQIAEAFPSEFGDEGLPIGWKFN
                     QADNLFDVGIGKTPPRKESEWFSDNANDTEWISIKDMGNQGLFITESSEYL"
BASE COUNT          156 a           96 c          101 g          109 t
ORIGIN      
        1 gtattgctaa ttattttgcc tttgaccaaa aaatccaact caacacccaa accaaccaaa
       61 ccctagaagc cattgcacag gcaatcttca aaagctggtt tgtggatttt gaccctgtgc
      121 gcgccaaagc cgccgcccta agcgaaggca aaagcgaaca cgaagccaac cttgcggcaa
      181 tgtcggtgat ttgtggtaaa gacaccagcg agttaaacga caccgaatac aaagcgcttt
      241 ggcaaatcgc cgaagccttc ccaagtgagt ttggagatga agggttacct attggctgga
      301 aattcaatca agcagacaat ttatttgatg ttggtattgg gaaaacccca ccaagaaaag
      361 agagtgaatg gttttctgat aatgcaaatg atacagaatg gatttctatt aaagatatgg
      421 gaaatcaggg attatttatc acagaaagta gcgaatatct aa
//
LOCUS       DANLZC010000283          460 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.287, whole genome shotgun sequence.
ACCESSION   DANLZC010000283 DANLZC010000000
VERSION     DANLZC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.287"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            111..>460
                     /locus_tag="PXE44_002603"
     CDS             111..>460
                     /locus_tag="PXE44_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HDL5590046.1"
                     /translation="MSFEQLLHLFFLERNHRPATIRSYQNVIAQIVKFYPDKEPEQIS
                     KLDLLEWRKYCLNKMSPITWNSYIRHLKAIFNYAIDNELFEYPKNLFKGLMVRPDKPK
                     KKLLTERQIHFLEIS"
BASE COUNT          173 a           66 c           69 g          152 t
ORIGIN      
        1 aacttaaatt gaattaactt taacttaaaa gatcaaggct aattcaagga gatctagtct
       61 acattaaatt gagtgctatt tgaagatcgc ttatctaaat gaggttttgt atgagttttg
      121 aacaattatt gcatttattt ttcttagaac ggaatcatag acctgccact atacgttctt
      181 atcaaaatgt aatagcgcag attgttaaat tttatccaga taaagaacct gaacaaatat
      241 ctaagcttga cttattagaa tggagaaagt attgcctcaa taaaatgagc ccaattactt
      301 ggaatagtta tatcagacat cttaaagcta tttttaatta tgctatagat aatgagctat
      361 ttgagtaccc taaaaattta tttaaaggcc ttatggtaag acctgataag ccaaagaaaa
      421 aactactaac agaaaggcaa atccattttt tagaaatctc
//
LOCUS       DANLZC010000284          447 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.288, whole genome shotgun sequence.
ACCESSION   DANLZC010000284 DANLZC010000000
VERSION     DANLZC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.288"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(91..192)
                     /locus_tag="PXE44_002604"
                     /pseudo
     CDS             complement(91..192)
                     /locus_tag="PXE44_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          139 a           75 c           68 g          165 t
ORIGIN      
        1 ataaatagcg gtaactatgt caccgctatt ttttatattt tcctaattat ctagctactt
       61 tatctgtgga tttttgcgta gattgctgaa cattcttact accaccactg cttgagagat
      121 attgcccagt tcttctgcta aatttaataa accggtcttg tgtttaataa ggagattgtt
      181 agaataaaac atgagagttt ccttttttgt ttagattgaa tttggacact catattctaa
      241 acgggaaact ctcacttttt agagtgaatt gtcagatcaa gtctgatctt ctacagccac
      301 cataaaaaac aatctcttca ttggttaaca aaccaataga ttttatgcta acaactcttt
      361 ccccatttgg aaaatcattc atcttcttta aattaagtag tgaattggga atgaaaacat
      421 gattttggtt aattaaggtt aatgcaa
//
LOCUS       DANLZC010000285          405 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.289, whole genome shotgun sequence.
ACCESSION   DANLZC010000285 DANLZC010000000
VERSION     DANLZC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.289"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..>405
                     /locus_tag="PXE44_002605"
     CDS             <1..>405
                     /locus_tag="PXE44_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5590047.1"
                     /translation="LVTAIPNFLEKVNNDFAISARNRDLYSHYLPYQGIDDWENSNFN
                     FRTLNGVYYDDNLTSTELLRKFMIQRDYNTLYALSSARTGNADSLAKNYHLVVAKMSY
                     ENLKKELSKKPDSTFNKLKKLYETNGEFLNEYI"
BASE COUNT          147 a           66 c           64 g          128 t
ORIGIN      
        1 ttagtaacag ccattcctaa cttcctggag aaagttaata atgattttgc tataagtgct
       61 agaaatagag acttatatag tcactacctt ccttatcagg gtattgatga ttgggagaat
      121 tctaatttta actttcgaac actaaatgga gtttattatg atgataatct aacttcaaca
      181 gaactcctgc gaaaatttat gattcaaaga gattataaca ctctttatgc tctaagttct
      241 gctcgcacag gaaatgctga tagtctggct aaaaattacc atcttgtagt tgcgaaaatg
      301 tcctatgaaa acctgaaaaa agaattatcc aagaaacccg actctacatt taataaactg
      361 aaaaagctgt atgaaaccaa tggtgaattc ttaaatgaat atatt
//
LOCUS       DANLZC010000286          404 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.290, whole genome shotgun sequence.
ACCESSION   DANLZC010000286 DANLZC010000000
VERSION     DANLZC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.290"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            10..357
                     /locus_tag="PXE44_002606"
     CDS             10..357
                     /locus_tag="PXE44_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590048.1"
                     /translation="MFREEAIEYQSKKWKSTAVLFSRVPALVVFSISFFIFTAFILFI
                     VFGNYTRRETVVGELVMQSHPILLSAPKSGYISENYIQPHQQVKKRAIRWLKLRWIEL
                     RIAKILMLILFFR"
BASE COUNT          129 a           70 c           70 g          135 t
ORIGIN      
        1 tggtattata tgttcagaga agaagctatt gaatatcaat cgaaaaaatg gaaaagcaca
       61 gctgtactat ttagcagagt tccagctttg gttgtctttt ccatttcttt ctttatcttt
      121 actgccttta ttctatttat tgtgtttggt aattataccc gccgtgaaac ggttgtgggt
      181 gaattagtga tgcaatctca tccgatttta ttatctgccc caaaatccgg ctatatatcc
      241 gaaaattata ttcaaccaca tcagcaggta aaaaaaaggg cgatccgctg gttaaaatta
      301 cgctggatag aattacgcat agcgaaaata ttaatgttaa ttctattctt tcgttgaaat
      361 cacaaattca agcaacagaa caagcgataa ctgctttata taag
//
LOCUS       DANLZC010000287          400 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.291, whole genome shotgun sequence.
ACCESSION   DANLZC010000287 DANLZC010000000
VERSION     DANLZC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.291"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..>400
                     /locus_tag="PXE44_002607"
     CDS             <1..>400
                     /locus_tag="PXE44_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590049.1"
                     /translation="LINGLEDIIQILNSSELSLFNGKVLVLTGQAGSGKSHLLGDFVQ
                     KRQNSGKYSLLLLGQDFTEKSNAWNQILSKQLNLNITPEVFLSALDSIGEMQKERVIL
                     AIDALNEGEGKSLWNNQLNGLIELVSQYPN"
BASE COUNT          141 a           50 c           74 g          135 t
ORIGIN      
        1 atttaattaa tggtttggaa gacattattc agattttaaa ttcatctgaa ttatcattat
       61 ttaatggtaa agtgcttgtt ttaactggtc aagctggtag tggaaaatct catttattgg
      121 gtgattttgt ccaaaaaaga caaaattctg gaaagtattc tttgttgtta ttggggcagg
      181 attttacaga aaaatccaat gcttggaatc aaatcttatc caaacaatta aatcttaata
      241 tcacacccga agtattttta tccgcattag atagtatcgg agaaatgcaa aaagaacggg
      301 ttattttagc catagacgcc ttaaatgagg gtgaagggaa atcattatgg aataaccaac
      361 ttaatggatt aattgagttg gtaagtcaat atccaaatat
//
LOCUS       DANLZC010000288          394 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.292, whole genome shotgun sequence.
ACCESSION   DANLZC010000288 DANLZC010000000
VERSION     DANLZC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.292"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(<1..>394)
                     /locus_tag="PXE44_002608"
     CDS             complement(<1..>394)
                     /locus_tag="PXE44_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HDL5590050.1"
                     /translation="DSVLIELLNCFEKRDDKRIEELVTDEAAIPTIFEYILGIIWYKV
                     SERQGNILDFMKLSLEANLLPKTHAAGGYADIIYEYEACTSYPKHSLLLEATLADGNN
                     QRRMEMEPVSRHLGDYRIRFNNPFDYSL"
BASE COUNT          121 a           82 c           56 g          135 t
ORIGIN      
        1 aataggctgt aatcaaacgg attattaaat ctaatcctat aatcgcctaa atgtcttgaa
       61 acaggctcca tttccattct tctttgatta tttccatctg caagggttgc ttcaagcaat
      121 aatgaatgtt tcggataaga ggtgcacgct tcatattcat atataatgtc tgcgtaacct
      181 ccagctgcgt gggtcttcgg caataaattg gcttctaatg ataacttcat gaaatccaaa
      241 atatttcctt gtctttcgct cactttgtac catataattc ccaaaatata ttcaaaaata
      301 gtaggtattg cagcctcatc tgtaacaagt tcttcaatcc ttttatcatc acgtttctca
      361 aaacaattta gtaactctat aagcacagaa tcgt
//
LOCUS       DANLZC010000289          376 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.293, whole genome shotgun sequence.
ACCESSION   DANLZC010000289 DANLZC010000000
VERSION     DANLZC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.293"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..>376
                     /locus_tag="PXE44_002609"
     CDS             <1..>376
                     /locus_tag="PXE44_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5590051.1"
                     /translation="AEEKGELVAVVHSHPEPNGKPILSVLDRKMQVQTGLDWWLVHNR
                     QIHKFRNVPHLIGREFKHGTMDCYTLYRDAYMLAGYEMDEFERQDDWWHTGQNLYLDN
                     IQGQGFERVETPQIGDVILMQVG"
BASE COUNT          110 a           76 c           99 g           91 t
ORIGIN      
        1 gcagaagaaa aaggcgaact ggtagcggtg gtacattcac atcccgagcc aaacggcaag
       61 cctatattat ctgtgcttga tcgcaaaatg caggtacaga ccggtttaga ttggtggcta
      121 gtgcataatc ggcaaatcca taaattccgc aatgtaccac atttaatcgg gcgtgagttt
      181 aaacacggca caatggattg ttacacgctc taccgtgatg cctatatgct tgccggttac
      241 gagatggacg agttcgagcg acaagatgac tggtggcata ccgggcaaaa tctttatttg
      301 gataatatcc aagggcaagg ttttgagcga gtggaaaccc cacaaatcgg cgatgtgatt
      361 ttaatgcaag taggtg
//
LOCUS       DANLZC010000290          376 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.294, whole genome shotgun sequence.
ACCESSION   DANLZC010000290 DANLZC010000000
VERSION     DANLZC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.294"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..>376
                     /locus_tag="PXE44_002610"
     CDS             <1..>376
                     /locus_tag="PXE44_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5590052.1"
                     /translation="SPLASKNLTEWKLLWQQYLDFYQVNLADDIIQSTWQKVSESENV
                     KGFGAFVDNRMVGFVHIVIHPNTWNLTECCYLEDLFVLAEYRNQGIGKELIEYIYRYA
                     QEKGYNRVYWVTDKNNHQAQQLY"
BASE COUNT          133 a           49 c           73 g          121 t
ORIGIN      
        1 ttcgccgtta gcctccaaaa atctcactga atggaaacta ttgtggcaac aatatttaga
       61 tttttatcaa gtgaatttag cagatgatat tattcaatca acttggcaga aagtttcaga
      121 gagtgaaaat gtaaagggat ttggggcttt tgttgataac agaatggttg gttttgttca
      181 tattgtgatt cacccaaata cttggaattt aactgaatgc tgttatttgg aagatttatt
      241 tgttttagct gaatatcgca accaaggcat aggaaaagaa ttaattgaat atatttatcg
      301 atatgcacag gaaaaaggat ataaccgagt atattgggta acagataaaa acaatcatca
      361 agcacaacag ctttat
//
LOCUS       DANLZC010000291          375 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.295, whole genome shotgun sequence.
ACCESSION   DANLZC010000291 DANLZC010000000
VERSION     DANLZC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.295"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            complement(138..>375)
                     /locus_tag="PXE44_002611"
     CDS             complement(138..>375)
                     /locus_tag="PXE44_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5590053.1"
                     /translation="DLRHSHVAYLIEQGFSPVVIAERLGHESISITLNYAHLYLGRDK
                     TEKKESVIGAIKKHKRKIDKTIEQSHSMSNDMEL"
BASE COUNT          112 a           73 c           42 g          148 t
ORIGIN      
        1 ccttgccaca tttcaatcta ttcagtagac attatagcat attttacctg ttttttctat
       61 atttttggct aaaaacatga tgctaattgc ttcaaaaaaa tggagagtat caaatttata
      121 atactctcca tgtatctcta tagctccata tcgttactca tactatgact ctgctcaatt
      181 gttttatcta ttttcctttt atgtttctta attgctccaa taactgattc ctttttctct
      241 gttttatccc taccaagata taaatgtgca tagtttaagg ttattgagat actttcatga
      301 cctaatcttt ctgcaattac cacaggagaa aatccttgct ctatcaaata agcaacatga
      361 gaatgtctta aatca
//
LOCUS       DANLZC010000292          367 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.296, whole genome shotgun sequence.
ACCESSION   DANLZC010000292 DANLZC010000000
VERSION     DANLZC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.296"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            93..>367
                     /locus_tag="PXE44_002612"
     CDS             93..>367
                     /locus_tag="PXE44_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDL5590054.1"
                     /translation="MKNRIEHPKIFISYAWGSQEHDEKVIALATNLKGDGIDVIFDKW
                     QLKEGNDTFKFMEKSVLDESITNVLILIDPIYAKKANERAGGVGTET"
BASE COUNT          142 a           39 c           82 g          104 t
ORIGIN      
        1 aaaagaggct actggagagt ataagtcata gtgattataa gatagtgaga aagaatactt
       61 attttgaaag gaatacatgg gaattgatac agatgaagaa tagaattgaa catcctaaga
      121 tttttatttc atatgcttgg ggatcacagg aacatgatga gaaggttatt gctttagcta
      181 caaatcttaa aggagatggt atagatgtca tttttgataa atggcagcta aaagaaggca
      241 atgatacctt taaattcatg gaaaaaagtg ttttagatga gagtattacc aatgtactga
      301 ttcttataga tcctatttat gcaaagaaag caaatgaaag agccggtggc gtaggtacag
      361 aaacaca
//
LOCUS       DANLZC010000293          367 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.297, whole genome shotgun sequence.
ACCESSION   DANLZC010000293 DANLZC010000000
VERSION     DANLZC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.297"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..>367
                     /locus_tag="PXE44_002613"
     CDS             <1..>367
                     /locus_tag="PXE44_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDL5590055.1"
                     /translation="NELIKSIKGDLLYLDPPYNSRQYCDAYHLLENVARWEKPKVYGV
                     ARKMDRTSLKSDYCMIAATKAFEELIENADAKYILLSYNNMSDKGNDRSNAKISDEDI
                     MKILSKKGKVIVFESDYKSF"
BASE COUNT          143 a           37 c           71 g          116 t
ORIGIN      
        1 aatgaattaa ttaaaagtat taaaggagat ttattgtatt tagaccctcc atataattca
       61 agacaatatt gtgatgcata tcatttactt gaaaatgtgg cacgatggga aaaacctaaa
      121 gtttatggtg ttgcaagaaa gatggataga acttcgctta aaagtgatta ttgtatgatt
      181 gcagctacaa aagcatttga ggagcttatt gaaaatgctg atgcaaaata tattttgctt
      241 tcatataaca atatgtcaga caagggaaat gatagatcca atgcgaaaat atcggatgaa
      301 gatattatga aaattcttag caaaaaggga aaagtaattg tatttgaatc ggattataag
      361 agttttt
//
LOCUS       DANLZC010000294          366 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.298, whole genome shotgun sequence.
ACCESSION   DANLZC010000294 DANLZC010000000
VERSION     DANLZC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.298"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..>366
                     /locus_tag="PXE44_002614"
     CDS             <1..>366
                     /locus_tag="PXE44_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5590056.1"
                     /translation="KSYIEVNHILKNIENDKINFNHQQNKYDSIINKAKNQNSQENNL
                     TEINSNIQILAEKHQLTIESLQWNLEQGKSIELSIIGNSRSLFDFIHSVKQIPYLKYN
                     AISLIKSRQDRKVEMNTTL"
BASE COUNT          163 a           56 c           46 g          101 t
ORIGIN      
        1 taaaatccta tattgaggtt aatcatattt taaaaaatat agaaaatgac aagattaact
       61 ttaatcatca acaaaacaaa tatgactcaa ttatcaataa agctaaaaat cagaacagcc
      121 aagagaataa tttaaccgaa ataaacagca acatacaaat attagctgaa aaacaccaac
      181 taaccattga aagtttacaa tggaatttag aacaaggtaa aagtattgaa ttgagtatta
      241 tcggcaatag ccgatcttta tttgatttta tccattcagt taagcaaata ccttatttga
      301 aatacaatgc gattagcctc atcaaatctc ggcaagacag aaaagttgaa atgaatacta
      361 ccttag
//
LOCUS       DANLZC010000295          354 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.299, whole genome shotgun sequence.
ACCESSION   DANLZC010000295 DANLZC010000000
VERSION     DANLZC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.299"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            31..107
                     /locus_tag="PXE44_002615"
     tRNA            31..107
                     /locus_tag="PXE44_002615"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:65..67,aa:Ile,seq:gat)
     gene            164..239
                     /locus_tag="PXE44_002616"
     tRNA            164..239
                     /locus_tag="PXE44_002616"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Ala,seq:tgc)
BASE COUNT          107 a           75 c           90 g           82 t
ORIGIN      
        1 aagaagacaa aaaaccgaag gtcatccgtt gggtctgtag ctcaggtggt tagagcgcac
       61 ccctgataag ggtgaggtcg gtggttcaag tccactcaga cccaccactc agaaggcaag
      121 cgaaagcgaa gcgactatga gtgaagaaaa cggaagatga aatggggata tagctcagct
      181 gggagagcgc ctgccttgca cgcaggaggt cagcggttcg atcccgctta tctccaccaa
      241 tcatcgtgac tttatcatca tacctaagat tacaagcggt taaaaatacc ggattatttg
      301 caattttagg tatgatgata ggcgaaagcc attatctttg ttctttaaca atat
//
LOCUS       DANLZC010000296          353 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.300, whole genome shotgun sequence.
ACCESSION   DANLZC010000296 DANLZC010000000
VERSION     DANLZC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.300"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            <1..>353
                     /locus_tag="PXE44_002617"
     CDS             <1..>353
                     /locus_tag="PXE44_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5590057.1"
                     /translation="QLRTDFLDPFFGLLGWDITNSKNKLTSEREVIVEEPLKEKVSEN
                     VKKPDYTFRFNSQRKFFVEAKKPSVKIKTDPLPAHQVRRYGFTAKLSISVLSNFEYLA
                     IYDCSEIVDVQQDAL"
BASE COUNT          131 a           54 c           56 g          112 t
ORIGIN      
        1 ctcagttaag aactgacttt ttagaccctt tctttggttt acttggatgg gatataacta
       61 actcaaagaa taaactcaca tctgaaagag aagtgattgt tgaagaacca ctaaaagaaa
      121 aagtttcaga aaatgtaaaa aaaccagatt atacatttag attcaattct caaagaaagt
      181 tttttgttga agctaaaaag ccaagtgtca aaatcaagac tgacccttta cctgctcatc
      241 aggtaagacg atatggcttc acagcaaaat taagtatttc tgttttatct aattttgaat
      301 atcttgctat atatgattgt tcagaaatag ttgatgtaca acaagatgct cta
//
LOCUS       DANLZC010000297          345 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.denovo.301, whole genome shotgun sequence.
ACCESSION   DANLZC010000297 DANLZC010000000
VERSION     DANLZC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.denovo.301"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
BASE COUNT          105 a           61 c           51 g          128 t
ORIGIN      
        1 atttctttcc ttcttgacta cctcttgcgt tgtagaaagc tcggtggata ataaataagt
       61 gtgttagata ttactggcac actatctatt tttgcccttc ttaacgtgga taatacggtt
      121 tttattgcta actcaaaatc attaacaaga atactctctt tgtactcatt cccactttgt
      181 cgtacaaaat caatctggta tgagcctatt ttatctaagc tgcacatttc ttgaatctgc
      241 tctaaatata tcactgaatg gttgtttgga tctcttaatc ttgattcttg aaaaaggaat
      301 aaacctatta caattgctga aaaaagaata attagtaaga cgtta
//
LOCUS       DANLZC010000298         2826 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.guided.207, whole genome shotgun sequence.
ACCESSION   DANLZC010000298 DANLZC010000000
VERSION     DANLZC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.guided.207"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            351..1826
                     /gene="msr(E)"
                     /locus_tag="PXE44_002618"
     CDS             351..1826
                     /gene="msr(E)"
                     /locus_tag="PXE44_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(E)"
                     /protein_id="HDL5590058.1"
                     /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKS
                     SLLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSG
                     GEETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLD
                     MVVDKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQ
                     QANRLDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPE
                     HLRSIRFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTG
                     KTSLLKMISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVL
                     ASMGIGANDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETM
                     MKSYAGTIIFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC"
     gene            1882..2766
                     /locus_tag="PXE44_002619"
     CDS             1882..2766
                     /locus_tag="PXE44_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="HDL5590059.1"
                     /translation="MTIQDIQSLAEAHGLLITDKMNFNEMGIDFKVVFALDTKGQQWL
                     LRIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAE
                     TYEIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLV
                     KSEIGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAH
                     IDDPAIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFAL
                     ETQNESLIVGAKAQLGVI"
BASE COUNT          961 a          460 c          592 g          813 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc taaaataagc cttatatcca
       61 agcataaaac aaggttgtct agacttcttt taacagtaaa gttatcataa aactgaattt
      121 tattttttag gtaagtttat gcattctatc cgcattcgtt aagacacaac tatttgcata
      181 gtgacactat tttataatgg tgggcttttg ttgtgtcttt aagaatatat gcggatatat
      241 aaagtaaaag tatgcttaat ttataagtat gcttttagtg catagtttcc agttataact
      301 taattgacta gctattaaaa atttcaagag ctaaacagga gtaaataaaa atgagtttaa
      361 ttattaaagc gagaaacata cgcttggatt atgctgggcg tgatgttttg gatattgatg
      421 aattggaaat tcactcttat gaccgtattg gtcttgtggg tgataacgga gcaggaaaga
      481 gtagtttact caaagtactt aatggcgaaa ttgttttagc cgaagcgaca ttacagcgtt
      541 ttggtgattt tgcacatatc agccaactgg gcggaatcga aatagaaacg gtcgaagacc
      601 gggcaatgtt atctcgcctt ggtgtttcca atgtacaaaa cgacacaatg agtggcggag
      661 aggaaactcg tgcaaaaatt gctgccgcat tttcccaaca agtacatggc attctagcgg
      721 atgaaccaac cagccacctt gatctcaatg gaatagatct acttattggt caacttaaag
      781 catttgatgg agcattactt gttatcagtc atgaccgata ttttcttgat atggttgtag
      841 acaagatatg ggagttaaaa gacggtaaaa ttacggaata ttggggtggt tactcggatt
      901 acttgcgtca aaaagaagaa gagcgacaac accaagccgt agaatatgag ctgatgatga
      961 aggaacggga gcgattagaa tctgctgtgc aagaaaaacg ccagcaagct aatcgattag
     1021 acaataagaa aaaaggagaa aaatccaaaa actctaccga aagtgctgga cgacttgggc
     1081 atgcaaaaat gactggcacc aagcaaagaa aactgtatca ggcagctaag agtatggaaa
     1141 agcgtttggc tgcattagaa gatattcaag caccagagca tttgcgttct attcgttttc
     1201 gtcaaagttc agccctagaa ctgcacaata agttcccgat tacggcagat ggtctgagct
     1261 taaaatttgg tagccgtact atctttgatg acgctaactt tataataccg cttggcgcta
     1321 aagtcgctat aactggatcg aatggaacag ggaaaacgtc cttgttaaaa atgatatcag
     1381 aacgtgctga tggattaacc atatctccaa aagctgaaat tggctacttt acacaaacag
     1441 gatataaatt taacacgcat aaatctgtgc tctcctttat gcaggaagag tgcgagtaca
     1501 cagttgcgga aattcgtgca gtattggctt caatggggat cggagcgaat gatattcaaa
     1561 aaaacttatc cgacttatcg ggaggtgaaa tcatcaaact gcttttatcc aaaatgcttt
     1621 taggaaaata taatattttg cttatggatg aaccaggaaa ctatcttgac ctaaaaagta
     1681 ttgccgcatt agaaacaatg atgaagtcct atgcaggaac tattatcttc gtatctcatg
     1741 acaagcaatt ggtcgataat attgctgaca ttatctacga gatcaaagac cacaaaatca
     1801 tcaagacttt tgagagagat tgttaatgat agccaatcta atccgaacat taattattga
     1861 actctttaaa ggaaattaaa aatgacaatt caagatattc aatcacttgc tgaagcacac
     1921 ggcttgttga ttacggacaa aatgaatttc aatgaaatgg gcattgattt taaggtcgtt
     1981 tttgctcttg atacaaaggg gcaacaatgg ttgctgcgta ttcctcgtcg tgatggcatg
     2041 agggaacaaa tcaagaaaga aaaacgcatt ttagaattgg taaaaaaaca tctttctgta
     2101 gaggttcctg attggagaat ttcatctaca gaattagtgg cttatcccat acttaaagat
     2161 aatcctgttt taaatttgga tgctgaaacc tatgaaataa tttggaatat ggacaaagat
     2221 agcccgaaat acataacatc tttggcaaaa accttatttg aaatccatag tattcctgaa
     2281 aaagaagttc gggaaaatga tttgaaaatt atgaaacctt cagatttaag acctgaaata
     2341 gcaaacaatt tgcagttagt aaaatctgaa attggtataa gtgagcaatt ggaaacccgc
     2401 tacagaaaat ggttggataa tgatgttcta tgggcagatt tcacccaatt tatacatggc
     2461 gatttatatg ctgggcatgt actagcttca aaggatggag ctgtttcagg cgttattgat
     2521 tggtcaacag cccatataga tgacccagcg attgattttg ctgggcatgt aactttgttt
     2581 ggagaagaaa gcctcaaaac tctaatcatc gagtatgaaa aactaggggg taaagtttgg
     2641 aataaactat atgaacagac tttagaaaga gcagcggcct ctcctttgat gtatggttta
     2701 tttgccttag aaactcaaaa tgaaagcctt atcgttggag caaaagctca gttgggagtt
     2761 atataattta aaaatatgat tgctgaggca ctgttgcaaa tagtcggtgg tgataaactt
     2821 atcatc
//
LOCUS       DANLZC010000299         2447 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.guided.220, whole genome shotgun sequence.
ACCESSION   DANLZC010000299 DANLZC010000000
VERSION     DANLZC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.guided.220"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            94..627
                     /gene="aadB"
                     /locus_tag="PXE44_002620"
     CDS             94..627
                     /gene="aadB"
                     /locus_tag="PXE44_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="HDL5590060.1"
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDD
                     IDLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEA
                     PQGSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLG
                     AEKVEVLRAAFRSRYAA"
     gene            685..1476
                     /locus_tag="PXE44_002621"
     CDS             685..1476
                     /locus_tag="PXE44_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA25"
                     /protein_id="HDL5590061.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKP
                     YSDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPA
                     QYQPVLLEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1553..2380
                     /locus_tag="PXE44_002622"
     CDS             1553..2380
                     /locus_tag="PXE44_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_034033256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-2"
                     /protein_id="HDL5590062.1"
                     /translation="MAIRIFAILFSIFSLATFAHAQEGTLERSDWRKFFSEFQAKGTI
                     VVADERQADRAMLVFDPVRSKKRYSPASTFKIPHTLFALDAGAVRDEFQIFRWDGVNR
                     GFAGHNQDQDLRSAMRNSTVWVYELFAKEIGDDKARRYLKKIDYGNADPSTSNGDYWI
                     EGSLAISAQEQIAFLRKLYRNELPFRVEHQRLVKDLMIVEAGRNWILRAKTGWEGRMG
                     WWVGWVEWPTGSVFFALNIDTPNRMDDLFKREAIVRAILRSIEALPPNPAVNSDAAR"
BASE COUNT          593 a          585 c          700 g          569 t
ORIGIN      
        1 aataaatcaa ttattattat actgctgatc accgtattag cagcaacgat gttacgcagc
       61 agggcagtcg ccctaaaaca aaggttaggc cgcatggaca caacgcaggt cacattgata
      121 cacaaaattc tagctgcggc agatgagcga aatctgccgc tctggatcgg tgggggctgg
      181 gcgatcgatg cacggctagg gcgtgtaaca cgcaagcacg atgatattga tctgacgttt
      241 cccggcgaga ggcgcggcga gctcgaggca atagttgaaa tgctcggcgg gcgcgtcatg
      301 gaggagttgg actatggatt cttagcggag atcggggatg agttacttga ctgcgaacct
      361 gcttggtggg cagacgaagc gtatgaaatc gcggaggctc cgcagggctc gtgcccagag
      421 gcggctgagg gcgtcatcgc cgggcggcca gtccgttgta acagctggga ggcgatcatc
      481 tgggattact tttactatgc cgatgaagta ccaccagtgg actggcctac aaagcacata
      541 gagtcctaca ggctcgcatg cacctcactc ggggcggaaa aggttgaggt cttgcgtgcc
      601 gctttcaggt cgcgatatgc ggcctaacaa ttcgtccaag ccgacgccgc ttcgcggcgc
      661 ggcttaactc aggtgttaga catcatgagg gaagcggtga ccatcgaaat ttcgaaccaa
      721 ctatcagagg tgctaagcgt cattgagcgc catctggaat caacgttgct ggccgtgcat
      781 ttgtacggct ccgcagtgga tggcggcctg aagccataca gcgatattga tttgttggtt
      841 actgtggccg taaagcttga tgaaacgacg cggcgagcat tgctcaatga tcttatggag
      901 gcttcggctt tccctggcga gagcgagacg ctccgcgcta tagaagtcac ccttgtcgtg
      961 catgacgaca tcatcccgtg gcgttatccg gctaagcgcg agctgcaatt tggagaatgg
     1021 cagcgcaatg acattcttgc gggtatcttc gagccagcca tgatcgacat tgatctggct
     1081 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     1141 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     1201 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     1261 atttggtaca gcgcaataac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     1321 ataaaacgcc tacctgccca gtatcagccc gtcttacttg aagctagaca ggcttatctt
     1381 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgttcactac
     1441 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga
     1501 cgccgcttcg cggcgcggct taccttggcc gttgggcatt aaggaaaagt taatggcaat
     1561 ccgaatcttc gcgatacttt tctccatttt ttctcttgcc actttcgcgc atgcgcaaga
     1621 aggcacgcta gaacgttctg actggaggaa gtttttcagc gaatttcaag ccaaaggcac
     1681 gatagttgtg gcagacgaac gccaagcgga tcgtgccatg ttggtttttg atcctgtgcg
     1741 atcgaagaaa cgctactcgc ctgcatcgac attcaagata cctcatacac tttttgcact
     1801 tgatgcaggc gctgttcgtg atgagttcca gatttttcga tgggacggcg ttaacagggg
     1861 ctttgcaggc cacaatcaag accaagattt gcgatcagca atgcggaatt ctactgtttg
     1921 ggtgtatgag ctatttgcaa aggaaattgg tgatgacaaa gctcggcgct atttgaagaa
     1981 aatcgactat ggcaacgccg atccttcgac aagtaatggc gattactgga tagaaggcag
     2041 ccttgcaatc tcggcgcagg agcaaattgc atttctcagg aagctctatc gtaacgagct
     2101 gccctttcgg gtagaacatc agcgcttggt caaggatctc atgattgtgg aagccggtcg
     2161 caactggata ctgcgtgcaa agacgggctg ggaaggccgt atgggttggt gggtaggatg
     2221 ggttgagtgg ccgactggct ccgtattctt cgcactgaat attgatacgc caaacagaat
     2281 ggatgatctt ttcaagaggg aggcaatcgt gcgggcaatc cttcgctcta ttgaagcgtt
     2341 accgcccaac ccggcagtca actcggacgc tgcgcgataa aaccgcgcag cgccggttac
     2401 ttcaacgggc actgttgcaa atagtcggtg gtgataaact tatcatc
//
LOCUS       DANLZC010000300         1351 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.guided.243, whole genome shotgun sequence.
ACCESSION   DANLZC010000300 DANLZC010000000
VERSION     DANLZC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.guided.243"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            47..1249
                     /gene="tet(H)"
                     /locus_tag="PXE44_002623"
     CDS             47..1249
                     /gene="tet(H)"
                     /locus_tag="PXE44_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDL5590063.1"
                     /translation="MNKSIIIILLITVLDAIGIGLIMPVLPTLLNEFVSENSLATHYG
                     VLLALYATMQVIFAPILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRI
                     IAGITGATGAVCASAMSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMP
                     FIFAAISHSILLILSLLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATY
                     FIIQLIGQIPATIWVLFTQYRFDWNTTSIGMSLAVLGVLNIFFQAIVAGKLAQKWGEK
                     TTIMISMSIDMMGCLLLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGK
                     LQGTLVSLTNITGIIGPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTT
                     PKAVISTP"
BASE COUNT          358 a          273 c          274 g          446 t
ORIGIN      
        1 agatatatta cacccactct atcactgata gatttttagg atctcaatga ataaatcaat
       61 tattattata ctgctgatca ccgtattaga tgccattggt atcgggctta tcatgccagt
      121 actccctact ctattaaatg aatttgtcag tgaaaattca ctggcaaccc attacggtgt
      181 gctattagcg ctctatgcta ccatgcaggt tatttttgct cctattctag gacgactgtc
      241 tgataaatac ggcagaaaac ccatcttgct gttttccctt ttaggcgcgg cactcgacta
      301 tcttttaatg gcattctcaa ccacactttg gatgctctat attgggcgca tcattgcggg
      361 gatcacaggc gcaacaggtg ccgtatgtgc atcagcgatg agtgatgtga ctcccgctaa
      421 aaatcgaact cgctattttg gtttcttagg tggtgctttt ggtgttggcc ttattatcgg
      481 cccaatgcta gggggattat taggtgatat cagtgctcat atgccattta tttttgccgc
      541 tatttcacac tcgatattat taatactctc tttgctcttt ttccgagaaa cacaaaaaag
      601 agaagcgctt gttgccaata ggacacctga aaaccaaact gcctcaaata cagtcactgt
      661 tttttttaag aaaagcctct acttttggtt agcaacctat tttattatcc agcttatcgg
      721 gcaaattcct gccaccatct gggtgctgtt tacacaatat cgttttgatt ggaacacaac
      781 ttctatcggt atgtctttgg cggttctggg tgtattaaat attttctttc aggcgattgt
      841 cgctgggaaa ttggcacaaa aatggggcga aaaaaccacc attatgatca gtatgtctat
      901 tgatatgatg ggctgtttat tattagcgtg gataggccac gtttgggtca tcttaccagc
      961 attaatttgc ttagcggcag gaggtatggg gcaacccgca ttacaaggtt atttatcaaa
     1021 atctgtcgat gataatgcgc aagggaaatt acaaggtact ctggtgagcc taaccaatat
     1081 taccgggatc attggtcccc ttttatttgc ctttatttat agttatagcg tcgcttattg
     1141 ggatggtctg ttatggctga tgggggcaat actttatgct atgttgctta ttaccgctta
     1201 ttttcaccaa agaaaaacca cacctaaagc tgttatttca accccttaat actagaaatt
     1261 ataaagatca taaaagtcct gtccgctctt aataacgttg ataggacttt atttgggtat
     1321 ccggattaca gggttgaaac agggctataa a
//
LOCUS       DANLZC010000301         1086 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25749909-rid16719633.guided.249, whole genome shotgun sequence.
ACCESSION   DANLZC010000301 DANLZC010000000
VERSION     DANLZC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25749909
            Sequence Read Archive: SRR17934057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574541.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574541.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 21:24:26
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,625
            CDSs (total)                      :: 2,573
            Genes (coding)                    :: 2,506
            CDSs (with protein)               :: 2,506
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 35 of 67
            Pseudo Genes (incomplete)         :: 22 of 67
            Pseudo Genes (internal stop)      :: 11 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25749909-rid16719633.guided.249"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: KY"
                     /collection_date="2020-10-02"
                     /collected_by="Murray State University Breathitt
                     Veterinary Center, Hopkinsville, KY"
     gene            11..484
                     /gene="gpt"
                     /locus_tag="PXE44_002624"
     CDS             11..484
                     /gene="gpt"
                     /locus_tag="PXE44_002624"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL5590064.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            597..>1086
                     /gene="gmk"
                     /locus_tag="PXE44_002625"
     CDS             597..>1086
                     /gene="gmk"
                     /locus_tag="PXE44_002625"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL5590065.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEF"
BASE COUNT          301 a          207 c          272 g          306 t
ORIGIN      
        1 ggttgtttct atgagtgaaa aatacacaaa tgaaaaatat gtcgtgacgt gggatatgtt
       61 ccatatgcac gctcgcaagt tggcagaaaa tttattacct gccacacagt ggaaagggat
      121 tattgcggtg agccgtggcg gcttgtttcc ggcagccgtt ttagcacgtg agttgggtat
      181 tcgccacgta gaaacagtgt gcattgccag ctacgatcac gatagccaag gtgagttaaa
      241 tgtgttacac gcagccgata ccgatggcga aggctttatt gtggtagatg atttagtgga
      301 taccggcaac acggcaaaag aaattcgcag aatgtacccg aaagctcgtt ttgtaaccgt
      361 gtttgcgaaa ccgcacggtg caccgttggt tgatgattat gtgattgata ttccacaaga
      421 aacgtggatt gagcagcctt gggatttagg cattaccttc gttcagcctt tggcacgtag
      481 ataattttgt gtaatggaca atgaaagttg tccattattt tttatttatt cctatgaaat
      541 gtaggggcga atcatcattc gccctattcc caaaatcaaa taggtatata caaataatga
      601 gtttaggcaa tctttatatt atttctgccc caagtggagc aggaaaatct tccttaatta
      661 acgccttgtt ggcggattta ccccgttcgg aagtacagct ttcgatttca cacactaccc
      721 gtcagcctcg tccggcagaa caggacggcg tgcattatta tttcacttcg cacgatgagt
      781 ttaagcagct gattgagcaa gggtattttt tagaatgggc ggaggtgttt ggcaattatt
      841 acggcacatc attgccgatg attgaacgta gcctagagca gggcattgat gtctttttgg
      901 atattgactg gcaaggggct agacaaattc gggaaaaagt gccgaatgtg aaaaccgttt
      961 ttattttgcc accgtcaaaa acagaattgg aaaaacgttt aatcgggcgt gggcaagatt
     1021 ccgctgaaac cattgccaaa cgtatggcag aggcggtgtc ggaaatgaac cattacaatg
     1081 agttcg
//
